LOCUS       DBMDVO010000001         9588 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_2526_length_9588_cov_1.208074, whole genome shotgun sequence.
ACCESSION   DBMDVO010000001 DBMDVO010000000
VERSION     DBMDVO010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_9588_cov_1.208074"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="ACZPE2_00005"
     CDS             <1..1312
                     /locus_tag="ACZPE2_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1318261.1"
                     /translation="YMLFCRNSFCGGIIYFAGEMYSRGYAHWEDKMKKVLNIILSVIL
                     CLSVVSPCFAIQSFAETGDETTLKFKDGKFRILVFADPQDDLNPCEETLDLMYASIEA
                     AKPDLVVFTGDNIHGPSPVLYRSKKRVEKAIRQIMEPVTSQNIPFAIVYGNHDDEGKV
                     SKQFQMEVYNSMPGCLASEGEAMTGCGNYNLPIKSSDGAKNVFNLWFIDSGTYDKENG
                     GYAHVAEDQLAWYKKTSDALKAENGGEPMPSLLFQHIPVPEIYELLKEVPKGTDGAVK
                     KDGKYYVLNTDIASGHLDEGPCPAQVNGGEFDAWKQQGDIKAAFFGHDHNNDFCGTYN
                     GIDMVHTSGVGFYIYGNGPLHGTRVIDIDENTLDYSTYMMYYNDLVGYKSSNKARYYE
                     GQYVHNIKIAAFSAGTVIIALTAGLITRGVKKSKAKKALKNNQK"
     gene            1549..>2469
                     /locus_tag="ACZPE2_00010"
     CDS             1549..>2469
                     /locus_tag="ACZPE2_00010"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1318262.1"
                     /translation="MKKDKKDIKKVVLAYSGGLDTSIIIPWLKENYNNCEVIAVSGDV
                     GQGTELDGLEEKALKTGASKLYVLDLKKDYIENYIWPCLKAGAEYEEYLLGTSHARPC
                     IAKGLADIAKKEGADAICHGCTGKGNDQVRFELALKALAPEMEIIAPWREWDIKSRDE
                     EIDYAEAHQIPLKINRETNYSKDKNVWHLSHEGLDLENPANEPQYNKDGFLELGVSPE
                     KAPDEPTYVTIHFEKGVPTAVDGEELDAVSLVEKLNKLGGENGIGLLDIVENRLVGMK
                     SRGVYETPGGTILYKAHELLEMITLDRDTSH"
     assembly_gap    2470..2479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2480..2753
                     /locus_tag="ACZPE2_00015"
     CDS             <2480..2753
                     /locus_tag="ACZPE2_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1318263.1"
                     /translation="GKWFSPLREALAAFVDNTQQTVTGDVKLKLYKGNVINAGVTSVY
                     TLYDENVASFGDDGGAYDQTDATGFINLFGLPTKVKALLDAKRDNK"
     gene            2862..>4049
                     /gene="argH"
                     /locus_tag="ACZPE2_00020"
     CDS             2862..>4049
                     /gene="argH"
                     /locus_tag="ACZPE2_00020"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1318264.1"
                     /translation="MQLWQGRFQKALDPKTNDFNSSIAFDSRMYKDDIDGSIAHATML
                     CAQNIISADDLSKIVDGLKAIKAEIENGTLEIDPNAEDIHTFVEGELTKRVGAAGKRL
                     HTARSRNDQVALDVRLYLRRECNSVYSLVTELIGVLCKKAEKYADAVMPGYTHMQRAQ
                     PITFGHHLLAYAEMLLRDRERIADAQRRMNYCPLGSCALAGTTYNTDREMTAEALGFT
                     GPMLNSLDGVSDRDFCMELASALSILMVHLSRFSEEIILWCSWEFKFVELDDAFSTGS
                     SIMPQKKNPDITELIRGKTGRVFGDLMTLLTMMKGLPLAYNKDMQEDKEAIFDAFDTV
                     KICLTTFIPMLDTMTAIPENMRKAAAGGFINATDCADYLVGKGLPFRDAYKATGEIVA
                     LCIK"
     assembly_gap    4050..4059
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4060..4251
                     /locus_tag="ACZPE2_00025"
     CDS             <4060..4251
                     /locus_tag="ACZPE2_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1318265.1"
                     /translation="GLTLETLPLEEYKKICDLFDDGVYDAINLDKCVNGRTSLGGPAP
                     QNVLKEAKRIEKILKEQDK"
     gene            4414..5346
                     /gene="argC"
                     /locus_tag="ACZPE2_00030"
     CDS             4414..5346
                     /gene="argC"
                     /locus_tag="ACZPE2_00030"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1318266.1"
                     /translation="MKKVFIDGSQGTTGLKIFDRLKKRDDIELLSLPDELRKDIKSRK
                     EALNSADIAFLCLPDAAAIEAAELVTNDDTVIIDTSTAHRTSEGWAYGFPEIFKDGFK
                     TVASSKRIASPGCYASGFIALVKPLLDEGIISPDHGFICHAVSGYSGAGKKGIAMYES
                     DEKAPELFAPREYALNQEHKHLKEMRKISGLSVTPIFCPYVCDYFSGMLVTVPVFKTD
                     LKDGKTAEDIKAVYRSKYASDIVKYKEAFDNGGFVAANSKAELDSMDVTVAGNDDRLL
                     LMALYDNLGKGASGAALECMNIGMGLPPEYGLDL"
     gene            5372..6592
                     /gene="argJ"
                     /locus_tag="ACZPE2_00035"
     CDS             5372..6592
                     /gene="argJ"
                     /locus_tag="ACZPE2_00035"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1318267.1"
                     /translation="MELSFIGGGVAAPKGFTANGIHCGIRKNKEKKDLALIYCEKDCD
                     AAAVYTQNLVCGAPITVTRENISDGKARAIVCNSGIANTCNADGVEKAEEMCKITADA
                     LGISKSDIVVASTGVIGQPIDLEPIKNGMAKLVSGLNKDGSDSAANAIMTTDTVKKEF
                     ACEFSLGGKKCRIGAISKGSGMIHPNMATMLAFITTDADISAEMLKKSLLEVVKDSFN
                     MLSVDGDTSTNDTVAVLASGLCGNEKITSENADYKAFTCALAAICEKLVKLMAKDGEG
                     ATKLVECIVSGAADQKTAKICAKSVICSSLVKAAMFGADANWGRILCALGYSGADIDV
                     HKVDVKFSSAGGEIEVCKDGSGIPFSEELAKKVLVCDEVYILVDLKSGSFSAAAYGCD
                     LTYDYVKINGDYRT"
     gene            6643..7509
                     /gene="argB"
                     /locus_tag="ACZPE2_00040"
     CDS             6643..7509
                     /gene="argB"
                     /locus_tag="ACZPE2_00040"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1318268.1"
                     /translation="MEISNITNSDRSEVLVQALPYIQKYQNKTVVIKYGGNAMVNEEL
                     QDAVMTDIVLLRSVGVNIVLVHGGGPEISDMLKRVGKQSRFVDGLRYTDEETIDIVQM
                     VLAGKVNKHLVQLISKHGGKAIGLCGIDGEMMKAVKYTKSDVGFVGEITEIDTDVIDY
                     TLENKYIPVISSVATGENGEVFNINADTAAAAIAASLHAEKLLLMTDISGLLMDKDDD
                     STLIHDVQVSEIPSLKSQGVISGGMIPKIDCCVEAVRRGVLQTNIIDGRIPHSILMEL
                     FTDEGFGTMFHG"
     gene            7764..8936
                     /locus_tag="ACZPE2_00045"
     CDS             7764..8936
                     /locus_tag="ACZPE2_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR1318269.1"
                     /translation="MDFNEIKNTADKYLMPTYGHFPIALTGGKNASLTASDGRVVIDF
                     TSGIGVNIFGVNDDGWKKAVIGQIDKIQHTSNLYYNEPVAKAAKLLCEKSGFDKVLFC
                     NSGAEANECAIKLARKYSFDRYGENRNTIISLVNSFHGRTMATITATGQEHYHKYFMP
                     FMGGFKYAEAGDKKAFSELLDGTVCAVILEVIQGEGGVVITPRDYIEYIRELCDENDI
                     VLIFDEVQTGVGRTGKLYAWENIGVKPDVLTSAKGLGGGLPIGACLAKEQFGSVLTPG
                     THGTTFGGNPVVLSGAVEILNRLDEEFLKDVREKGEYIRNKVSSFKDVSEVRGMGLMI
                     GIDLKNQKAADVAARCVENGLLILTAKQSLRMLPPLTITYDEIDRGLEILEKTLSE"
     gene            9022..>9588
                     /locus_tag="ACZPE2_00050"
     CDS             9022..>9588
                     /locus_tag="ACZPE2_00050"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1318270.1"
                     /translation="MKHLLSLLSTTPGEISDLLNLADQLKYENKHGIAHQRLKGQTLG
                     MIFQKSSTRTRVSFETGMYQLGGQALFLSNRDLQIGRGEPIQDTARVLSRYIDGIMIR
                     TFEQSEVEALAKYGSIPIINGLTDFCHPCQVLADLMTVREHKGHLDGLKMCYIGDGNN
                     MANSLIVGGLKTGMQVSVACPEGYRPDSR"
BASE COUNT         2757 a         2061 c         2340 g         2410 t
ORIGIN      
        1 ttatatgctg ttttgtcgca atagcttttg cggcggcatt atttattttg caggagaaat
       61 gtattcgaga ggatacgcgc attgggagga taaaatgaaa aaggtattaa acattatttt
      121 atcggtaatt ctttgtttat cggtggtttc accgtgcttt gcaattcaga gctttgccga
      181 aactggggat gaaactacgc ttaaatttaa agacggtaaa tttcgtatac tcgtatttgc
      241 agatccgcag gatgatttaa atccgtgcga agagacgctt gatcttatgt atgcttcgat
      301 tgaagctgca aagcccgacc ttgttgtatt taccggcgat aatatacacg gcccgtcgcc
      361 cgtgctttac agaagcaaaa agcgtgtcga aaaggcaatc aggcagatta tggagcccgt
      421 tacctcacag aacataccgt ttgcaatagt atacggtaac cacgatgacg agggtaaggt
      481 ctccaaacag ttccaaatgg aggtctataa ttctatgccg ggctgccttg cttccgaggg
      541 agaagctatg accggctgcg gcaactataa tcttcctata aaaagctcgg acggcgcaaa
      601 gaacgtcttt aatctttggt ttatagattc cggcacatat gataaagaaa acggcggata
      661 tgcccacgtt gccgaggatc aacttgcttg gtacaaaaaa acgagcgatg ccctgaaagc
      721 tgaaaacggc ggagaaccta tgccgtctct ccttttccag catatacccg ttcccgaaat
      781 atatgaactt ttaaaggagg ttcccaaggg cacggacggt gcggtaaaaa aagacggtaa
      841 gtattatgtg cttaatacgg atattgcaag cggtcatctt gacgaggggc cgtgtcccgc
      901 acaggtgaac ggcggcgaat ttgacgcttg gaagcagcag ggagacatta aggcggcgtt
      961 cttcgggcac gatcataaca atgacttttg cggcacgtat aatggcattg atatggtgca
     1021 tacgagcgga gtcggatttt atatttacgg taacggacct ctccacggga cgcgcgttat
     1081 agatatagac gaaaacactc tcgattacag cacttatatg atgtattata atgaccttgt
     1141 cggttacaag agctcaaaca aggcaagata ttacgagggg cagtatgtac ataacattaa
     1201 gatagcggct ttttcggcag gcactgtgat aattgccctg acggccgggc ttataacgcg
     1261 cggagtcaaa aagtccaagg cgaaaaaagc tttaaaaaat aaccaaaaat aacggctgag
     1321 ccgggcgaaa tttctcgctc ggctttagtt tttgcataaa aattcataaa tcaggttaaa
     1381 tattgcatag aacagggcac ataatgtata tttatgtcgt tatatcgcac aaatatacac
     1441 aaaacttgaa tatttatgca aaaaacactt gattttaaat gtgattggtg tataatctct
     1501 tcgtaatcaa aaattaaagg cttttgccga tgctcggagg taacatttat gaaaaaggac
     1561 aagaaagaca tcaaaaaagt cgtactcgct tattccggcg gacttgatac gtcaatcatc
     1621 attccgtggc ttaaagaaaa ttacaataac tgcgaagtta tagcggtttc cggcgacgta
     1681 ggtcagggaa ctgagcttga cggacttgag gagaaggctc tcaagaccgg cgcttcaaag
     1741 ctctatgttc tcgaccttaa aaaggattat atcgaaaact atatctggcc ttgccttaaa
     1801 gcaggtgccg agtatgaaga gtatcttctc ggtacatcgc acgctcgtcc ctgtatcgca
     1861 aagggccttg ccgatatagc aaaaaaagag ggcgcagacg ctatttgcca cggctgcacc
     1921 ggcaagggca acgatcaagt tcgtttcgag cttgctctta aagctcttgc tcccgagatg
     1981 gagattatag ctccctggcg tgagtgggac ataaaatcgc gtgatgaaga aatagactac
     2041 gccgaggctc atcagattcc gcttaaaatc aatcgtgaaa caaactactc aaaagataaa
     2101 aacgtatggc atctctctca cgagggactt gaccttgaaa atccggcaaa tgaaccgcag
     2161 tacaataaag acggctttct tgagctcgga gtttctcccg aaaaagctcc cgatgagcct
     2221 acatatgtta caatccattt tgaaaagggc gttcccacag ccgttgacgg cgaagagctt
     2281 gacgctgttt cacttgtcga aaagctcaat aagctcggtg gagaaaacgg tataggcctt
     2341 cttgatatag tcgagaaccg tcttgtaggt atgaaatcac gcggtgttta tgagaccccc
     2401 ggcggaacaa tcctctacaa ggctcatgag cttcttgaaa tgataactct cgaccgtgac
     2461 acatctcatn nnnnnnnnnc ggcaaatggt tctcacctct tcgcgaagct cttgcggctt
     2521 ttgtcgacaa cactcagcag accgtaaccg gcgacgttaa gcttaagctc tataagggca
     2581 acgttataaa cgcaggcgtc acctctgtgt atactcttta tgatgaaaat gttgcttcgt
     2641 tcggcgatga cggcggcgca tacgatcaga ccgacgctac cggatttatc aatctcttcg
     2701 gactccccac aaaggtaaaa gctttacttg acgcaaagag agataacaaa taatcattgt
     2761 aaaccgcttg aaaaaataaa caacagtgct tagcactacc gcgtgcgaca agagatgttt
     2821 ctgtctcttg tcgctgtgtg gtatttgata ggagtaaaaa aatgcagctt tggcagggca
     2881 gatttcaaaa ggctttagac cccaaaacca acgattttaa ttcgtcaata gcgtttgaca
     2941 gcagaatgta taaggatgat atagacggca gtattgcaca cgccactatg ctttgcgcgc
     3001 aaaatatcat atcggcggac gacctttcaa aaattgttga cggattaaaa gccattaagg
     3061 cggaaatcga aaacggtacg ttggagatag acccgaatgc agaggatata catacctttg
     3121 ttgagggtga gctcacaaag cgcgtaggcg cggccggcaa aagacttcat accgcgcgca
     3181 gcagaaacga ccaggtagct cttgacgtga ggctgtattt gcgccgcgag tgcaacagcg
     3241 tttattcgct tgtcacggag cttataggtg ttctgtgcaa aaaggctgaa aagtatgcgg
     3301 acgccgttat gccgggttac actcatatgc agcgcgctca gcccataaca ttcggccatc
     3361 atcttttggc gtatgccgaa atgcttctgc gtgaccgcga acgtattgcc gatgcgcaga
     3421 gaagaatgaa ttactgcccg ctcggctcat gtgcacttgc cggtacaaca tataataccg
     3481 accgcgaaat gacagccgag gcactcggat ttacgggacc tatgctcaat tcgcttgacg
     3541 gcgtatcgga cagggacttt tgtatggagc ttgcgtccgc tttgtcaata cttatggttc
     3601 atctctcgcg cttttctgag gagataatcc tctggtgctc gtgggaattt aaattcgttg
     3661 agcttgacga tgctttttca acaggctcgt caataatgcc gcaaaagaaa aaccccgaca
     3721 taaccgagct tatccgcggc aaaaccggtc gagttttcgg agaccttatg acgcttctga
     3781 caatgatgaa aggcttgccg ctcgcttata ataaggatat gcaggaggat aaggaagcta
     3841 tatttgacgc ctttgacacc gttaaaatct gcctcaccac atttatcccc atgcttgaca
     3901 caatgacggc tattcccgaa aatatgcgta aagccgcggc aggcggattt atcaacgcta
     3961 ccgactgcgc cgattatctc gtcggcaagg gattgccgtt ccgtgacgca tataaggcca
     4021 caggtgaaat agtggctctc tgcattaaan nnnnnnnnng gacttacgct tgaaacgttg
     4081 cctcttgaag aatacaaaaa gatatgcgat ttgtttgacg acggcgttta tgacgccata
     4141 aatctcgaca agtgcgtaaa cggcaggact tcgctcggcg gacccgcacc gcagaacgtg
     4201 ctgaaagagg caaagagaat agaaaaaata ttgaaagaac aagacaaata gtgtgaaaag
     4261 ttacacttct taaattatat tgccgtctca aaatttgcct gtggcataat tttgagacgg
     4321 caaaaatcac cgattgcgcc ccgctaaggc ttcaaagaat atacggtgat ttttaataaa
     4381 ctgtttggtg cctgaaaggc tatggtgaat aatatgaaaa aagtatttat agacggcagt
     4441 cagggaacta cgggtcttaa aatatttgac cgtctgaaaa aaagagatga tattgagctt
     4501 ttatctcttc ccgatgagct tcgcaaggac ataaaatcaa gaaaggaagc tctcaacagt
     4561 gccgatatag cttttctctg ccttccggac gctgcggcaa tcgaggcggc agagcttgtt
     4621 acaaatgacg acacggttat tatagataca tcaaccgctc accgtacaag cgaggggtgg
     4681 gcttacggtt tccccgaaat atttaaagac ggatttaaaa ccgtggcttc ttcaaagcgt
     4741 atagcgtcgc cgggctgtta tgcaagcgga tttatagcgc ttgtaaagcc tcttttggac
     4801 gagggtatta tttctccgga ccacggattt atctgtcacg ccgtttcggg ttacagcgga
     4861 gccggcaaaa aaggtatcgc tatgtatgaa agcgacgaga aagcaccgga gctttttgct
     4921 ccccgtgaat acgctttaaa tcaggagcat aagcacctca aagaaatgcg aaaaatttcg
     4981 ggcctgtcag ttacaccgat attttgtccg tatgtctgtg attatttcag cggtatgctc
     5041 gtcacggttc cggtgtttaa aacggatctc aaagacggaa aaaccgcaga ggacattaaa
     5101 gcggtttacc gctcaaaata tgcttcggac atagttaaat ataaagaggc ttttgataac
     5161 ggcggctttg tcgcggcaaa ttctaaagct gagcttgaca gtatggatgt caccgtagcc
     5221 ggaaatgacg acagacttct tcttatggct ctttacgaca atctcggcaa gggtgcgtcg
     5281 ggagcggctt tggaatgtat gaatattggt atgggtttac ctcccgaata cggacttgac
     5341 ctgtaagatt ataaatgaaa ggtaaattat aatggaatta agttttatcg gcggcggtgt
     5401 cgccgcaccg aaaggattta ccgcaaacgg aattcattgc ggaatccgta agaataagga
     5461 gaaaaaggat cttgcactta tttactgtga aaaggactgt gacgcggcgg ctgtttatac
     5521 gcaaaatctt gtgtgcggag caccgataac cgtcacacgt gaaaatataa gcgacggcaa
     5581 ggcgcgcgca attgtctgca acagcggcat agccaatacc tgcaatgccg acggagttga
     5641 aaaagcggag gaaatgtgca aaataaccgc ggacgcactc ggcatttcaa aaagcgatat
     5701 tgtggtagct tccaccggag ttataggtca gccaatagac cttgagccca tcaaaaacgg
     5761 tatggcgaag cttgtttcgg gccttaataa ggacggctct gattctgcgg caaatgcgat
     5821 aatgacaacg gacaccgtaa aaaaagaatt tgcctgtgag ttttctctcg gcggcaaaaa
     5881 atgccgtata ggtgctattt ccaagggcag cggtatgatt cacccgaata tggcaacgat
     5941 gcttgctttt attacaacgg atgccgacat ttcggcggaa atgcttaaaa aatcacttct
     6001 cgaggtggtt aaagacagct ttaatatgct ctccgtagac ggcgacacat ccaccaacga
     6061 taccgtggct gttttggcgt cgggactttg cggaaacgag aaaataacct ccgaaaacgc
     6121 agattataaa gcctttactt gtgcgcttgc ggcaatttgc gaaaagcttg taaagcttat
     6181 ggcgaaagac ggcgagggtg caacaaagct tgtcgaatgt attgtttcgg gtgccgctga
     6241 ccaaaagacg gcaaaaatat gtgcaaaatc ggttatatgc tcgtcgcttg ttaaggcggc
     6301 tatgttcgga gcggatgcaa actggggcag aattctctgc gctctcggtt acagcggagc
     6361 cgatattgac gttcataagg ttgatgtgaa attcagctcg gcaggcggag aaatagaagt
     6421 gtgcaaggac ggctcgggaa ttccgttttc cgaagaactt gcgaaaaaag tcttagtctg
     6481 tgatgaggtg tatatccttg tcgatttaaa atcaggcagc ttctctgccg cggcttacgg
     6541 ctgtgatttg acatacgatt acgtcaagat aaacggcgat taccgcacct gagcgtaaaa
     6601 cgcataaacg cagcattcac agacatttat cggaggagaa atatggaaat atcaaatata
     6661 acaaattcgg accgttccga ggttctcgtt caagccttac cgtatattca gaaatatcaa
     6721 aataaaacgg tcgtcataaa gtacggcggc aatgccatgg taaacgaaga gcttcaggat
     6781 gccgtaatga ccgacatagt tcttcttcgt tccgtcggag taaatattgt gcttgttcac
     6841 ggcggcggac cggaaatatc cgatatgctc aaacgtgtcg gcaagcagag ccgctttgta
     6901 gacggactgc gttataccga cgaagagaca atagatattg ttcaaatggt tttggcaggc
     6961 aaggtgaata agcatttggt acagctcatt tcaaagcacg gcggcaaggc gataggcctt
     7021 tgcggtatcg acggcgaaat gatgaaagcc gtaaaataca caaaatcgga cgtcggattt
     7081 gtaggcgaga taaccgaaat cgataccgac gttatagatt acactcttga aaataaatat
     7141 atacccgtta tctcatccgt tgcaacgggc gagaacggcg aggtgttcaa tataaacgcc
     7201 gataccgccg cggcggcaat agccgcaagt ctccacgcgg aaaagctttt gcttatgacc
     7261 gacatcagcg gacttttaat ggataaagac gatgattcca ctttaattca cgacgttcag
     7321 gtttccgaaa taccgtcgct caagtctcag ggcgttatct ccggcggtat gataccgaaa
     7381 atcgactgct gcgtagaggc cgtccgccgc ggagttcttc agaccaacat aatcgacgga
     7441 cgtataccgc attctattct tatggagtta ttcacggacg agggcttcgg cacaatgttt
     7501 cacgggtgat ttcgtgtctt ttttccgtta tacttcgttc tgtgcagctc gcagtatata
     7561 catacagctt cgccgcacac gccttgtcta acgaaaaaaa tttcacgaaa tcaagatttt
     7621 gcgctttttt ctgctaaact tcgttttgtg cagacagcaa gtatataaca tacagcatct
     7681 taaacaacag cttgcgttaa acgtcaattg catttttcgg actttgtata tataataaga
     7741 ttagtttatt aaggactgat aaaatggatt ttaacgaaat taaaaatact gccgacaaat
     7801 atttaatgcc gacttacggt cattttccga tagccttgac gggcggcaaa aatgcgtctc
     7861 tgacggcctc tgacggcaga gtggtaattg attttacttc gggtataggc gttaatattt
     7921 tcggcgtaaa cgatgatggc tggaaaaagg ctgttatcgg gcagatagat aaaattcagc
     7981 atacatcaaa tctttattat aacgagcctg ttgccaaggc ggcaaagctt ctgtgcgaaa
     8041 aaagcggatt tgataaggtg cttttttgca attccggagc ggaggcaaat gagtgcgcca
     8101 taaagcttgc gagaaaatac agctttgacc gctacggtga aaaccgaaat acgataatat
     8161 cgcttgtgaa ttctttccac ggcagaacca tggctacaat aaccgcaacg gggcaggagc
     8221 actaccacaa gtattttatg ccttttatgg gcggctttaa atatgccgag gcgggcgaca
     8281 aaaaagcgtt ttccgagctt ctcgacggaa cggtttgcgc ggtaatactt gaggttatac
     8341 agggcgaggg cggagtcgta ataactccgc gcgattatat agaatacata agagagcttt
     8401 gcgatgaaaa cgatattgtt ctcatattcg acgaagtgca gacgggcgtc ggcagaacag
     8461 gtaagctcta tgcttgggaa aatatcggcg taaagccgga cgttttgaca tccgcaaagg
     8521 gcctcggcgg cggactgccg ataggagcgt gccttgcaaa agagcagttc ggcagcgttt
     8581 tgactcccgg cactcacggt accaccttcg gcggaaatcc cgtggtttta tcgggcgcgg
     8641 ttgagatatt aaatcgactt gacgaagaat ttttaaagga cgtccgcgaa aagggcgaat
     8701 acataagaaa caaggtttct tcgtttaagg atgtttccga agtgcgcggt atgggcctta
     8761 tgataggtat agaccttaaa aatcaaaagg cggcggatgt ggcggcgcgc tgtgttgaaa
     8821 acgggcttct tattttaacc gccaagcagt cgcttcgtat gcttcctccg ctcaccataa
     8881 catatgacga aatagacagg ggacttgaga ttctcgaaaa gaccctttct gaataaactc
     8941 ctatgcagtt ttcacgcctt gcacggctac ggcaggggca acgtgactat atttgtagcc
     9001 ggaaaggctc tgtgaattaa aatgaagcat ttgttaagtc ttttatcgac aactcccggt
     9061 gaaatatctg accttttaaa tcttgccgat cagcttaaat atgaaaacaa gcacggcata
     9121 gcgcatcaaa ggctcaaggg tcagaccctc ggtatgattt tccaaaaatc ctcaacaaga
     9181 acaagggttt cttttgaaac cggtatgtat cagctcggcg gtcaggcact ctttctttca
     9241 aaccgcgatc ttcagatagg acgcggcgag cctatacagg ataccgcaag agttctctcg
     9301 cgctatatcg acggcattat gataagaacc tttgagcagt ccgaggttga ggcactcgcg
     9361 aagtacggtt ctatcccgat aattaacggt cttaccgatt tttgccatcc gtgccaggtg
     9421 cttgccgacc ttatgacggt acgtgagcac aaggggcacc ttgacggcct taaaatgtgc
     9481 tacataggcg acggcaacaa tatggcaaat tctcttatag tcggcggact taaaacaggt
     9541 atgcaggtat cggtggcttg ccccgagggg tacagacccg acagccgc
//
LOCUS       DBMDVO010000002         8694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_2936_length_8694_cov_1.196555, whole genome shotgun sequence.
ACCESSION   DBMDVO010000002 DBMDVO010000000
VERSION     DBMDVO010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2936_length_8694_cov_1.196555"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /gene="leuC"
                     /locus_tag="ACZPE2_00055"
     CDS             complement(<1..1241)
                     /gene="leuC"
                     /locus_tag="ACZPE2_00055"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1318271.1"
                     /translation="MSMTMTQKILAAHAGLQSVTAGQLIEAKLDLVLGNDITSPVAIN
                     EMAKRGFKDVFSTDKIALVMDHFTPNKDIKSAENVKQVRLFAKEKGIKNFFDVGKMGI
                     EHALLPESGLVISGDAVIGADSHTCTYGALGAFSTGVGSTDMAAGMISGKAWFKVPSA
                     IKVVLKGTLPEFVSGKDVILHLIGKIGVDGARYRSLEFTGDGVGALSMDDRFCIANMA
                     IECGAKNGIFPVDDRTLEYISGRTDRQPRIFEADEDAEYDETIEIDLNNLKPTVAFPH
                     LPENTHTVDEIEKIDIDQVVIGSCTNGRIEDMRAAASVLKGRKVKDGVRVIVIPATQK
                     VYLQCIKEGLTEIFVEAGAVVSTPTCGPCLGGHMGILAKGERAVSTTNRNFVGRMGHP
                     ESEVYLASPFVAAASAVTGYI"
     gene            complement(1246..1776)
                     /gene="ilvN"
                     /locus_tag="ACZPE2_00060"
     CDS             complement(1246..1776)
                     /gene="ilvN"
                     /locus_tag="ACZPE2_00060"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1318272.1"
                     /translation="MAEENNANSLERIFLSALVYNRTGVLLRVASLFARRGYNVISLT
                     VAETENPVYSRITIVSDVEPHKFLQVERQLSKLEDVIKVVKLNESRLVSSELLLIKVH
                     ATNSQRTAVLKTINGFGAKVKDIGHTTITAELTGLPSLIDKFIGKMTKHGIIELSRSG
                     LTALESGDTNIKGIEE"
     gene            complement(1801..2844)
                     /locus_tag="ACZPE2_00065"
     CDS             complement(1801..2844)
                     /locus_tag="ACZPE2_00065"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1318273.1"
                     /translation="MTKSLYGKTADGKEVFSFTIKNESGAEIELLNYGATLNKISVPD
                     RNGDFADVLVGFDTMEGQLSCTDSQGRTVGRVANRISGNGITIDGKNYPITKNIDGKF
                     TLHGNHEYENAVWNYEILGDDSVKFSYFSADGNEGFPSNLQNEVTFTFTDKNEVKIHY
                     DCRPDGKTPINVTNHSYFNLGGYASGNILSHEMQIFAKYFTPMNEDSIPTGEIRNVEN
                     TPFDFRIPKKIGRDVSSPDEQLIIGRGYDHNFCLENYTGALREFAMVYDPESGRKMTC
                     YTDLPGVQFYIGNYMDGSQIGKGKLPLTHRSGFCLETQYYPNALNNPDFIQNLFDENH
                     PFVSDTVYRFSAE"
     gene            3035..3769
                     /locus_tag="ACZPE2_00070"
     CDS             3035..3769
                     /locus_tag="ACZPE2_00070"
                     /inference="COORDINATES: protein motif:HMM:NF028007.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1318274.1"
                     /translation="MEQFLTQFEFLSRYALAFLALIILLRCIFSLFRLRPRREIMATL
                     INLADDSEIEIRNWETSIGRSRSCDISLRKYGTVSRFHAVLALRKDGWYVFDTESKTG
                     VYVNGEQIKKSAKIIDGDTVSFGNAVMKFTSNGYGVESEEAEDGKKVDFTNATRLVNL
                     ADNSAFYLNGTCLIGRDRTCNICLPMQSVSGEHAEVYPAKDGWYVADLNSKNGTLLNG
                     EEVFGAFPLYDGDIISIGDYSFLFRE"
     gene            3812..5101
                     /locus_tag="ACZPE2_00075"
     CDS             3812..5101
                     /locus_tag="ACZPE2_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1318275.1"
                     /translation="MAQENARKKQKKTRTKKYRKPVNWLILLIIMTVFQLVCAAQINM
                     NSPDIPLTTLYIVFGAYIAIEWAYFIFFGGFLHRKSFEIELIAFFLTGIGLTLTTSVY
                     PDKAFVQLAAVIMGIAVFIVMLWVLSDIERVVKLRMPVAIASVIVLVATRLLAHNING
                     AYNWIRIGGVSIQPSEIVKIGFVFVGAATLEYLQSTRSLTKYLVFALSCIGCLFLMKD
                     FGTALIFFFTFLIMAFLRSGDIKTIFLVCAAALGGGGLVLTFKPYVAKRFETYRHVWE
                     YANDRGYQQARLLIYSVSGGLFGLGIGNGKLRDVYAATEDLAFGMVCEEFGIIVAFAV
                     LLTFVALVVYSIRYAVAARSSFYAIAACAASGLLLFQAALHVFGVTDLLPWTGVTLPF
                     ISRGGSSMMCCWGLVAFIKAIDVKTYKRYDKVAVAKS"
     gene            5132..6532
                     /locus_tag="ACZPE2_00080"
     CDS             5132..6532
                     /locus_tag="ACZPE2_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1318276.1"
                     /translation="MKTMSKRAWVPYVFIAAFLAGLIVLFYTFYTNADSWAMKKANRH
                     LYSSGTLSVAGSIKDETGAVLAETKDGKRVYNSDKTVRTATMHTVGDSEGYIATGVQT
                     SYKDVITGYNFVDGIYNLKKYGKGNDVNLTINAQLCAAAYSALGSNKGTVGVYNYKTG
                     QLLCVVSSPSYDIANKPVSSINNDKTGAYEGVYLNRFFSGVYTPGSTFKMITSICAIE
                     NLPDIDSQKFKCTGEYKVGGSSVKCLGVHGTVNFEKALNVSCNSAFAQIAIELGPDKL
                     MATAKELGLGNQMYVGNIRLAKSSFNVTGASDLDVGWAGIGQYTTRINPCQMLTIAGA
                     VANGGSSVPPYLVGKITSQTGKTIYEAKPGTATQLISSDTASKMNDMLRSNVKNYYSD
                     GKFPNLKMCGKTGTAEVSSEAGGAKPHAWFVGYSQREDLPLAIVVVVENGGGGSSVAI
                     PVANKTMQKALSLYAK"
     assembly_gap    6733..6742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6743..7935
                     /locus_tag="ACZPE2_00085"
     CDS             <6743..7935
                     /locus_tag="ACZPE2_00085"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR1318277.1"
                     /translation="YSSSVIMKKNVELHLQKGSVLKATSDINGYFRPCDFINDETNTL
                     IGNPVTGKPSFAFIYAYKADNAAITGGGKIDANGHSFVKRKDKYYVTGDFYPRPTVMY
                     FEACNHIVFEDFTVVDAPFWTLHPAGCDDVLISKIHILNDLDVANSDGIDPDHCSNVR
                     ICDCHVECADDCICLKTSKGNSEYGPCENILITGCTLISTSAAIKIGTEGVGDFRNII
                     VSNCVISRSNRGLSIQIRDGGNVENVKYSDIIIGTRRFCPDWWGTAEPIVITAFDRDE
                     NTRAGHIKNISYRNITCVGENGVMICGTVENKIENVVFSDVDVTLSKTSKWDCGLYDM
                     RPGLNKEVEKHKNAGFYLRFADNVTLRNTSVKWGNVCPEYSAALEEESCVGTVLENFT
                     GDNA"
     gene            8121..>8694
                     /locus_tag="ACZPE2_00090"
     CDS             8121..>8694
                     /locus_tag="ACZPE2_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HIR1318278.1"
                     /translation="MKKALNKKVLSVLIAAVILLVTAFPVLAADPFKLTSSAEAKDSV
                     VTVKIYLPQNTELGTLSFTLKYDTKLLTYVSAEYSSGDVSATNSSTAGEVKINAVWKN
                     TAPLGGEVAAITFKVNDGATGKTDFPINVDFSTDANNQHIDLSAVSSSIDLSEAAPMP
                     AATDETVSSEGKTVKVDIDQHRQKMGMVFQH"
BASE COUNT         2206 a         1968 c         2168 g         2342 t
ORIGIN      
        1 catatatagc ccgtaacggc acttgccgcc gctacgaagg ggcttgcgag ataaacctcg
       61 ctctccgggt gtcccattcg gcccacaaag ttgcggtttg tggtggaaac ggctctttcg
      121 ccctttgcga gtatgcccat atgaccgccg agacacggtc cgcatgtcgg ggttgataca
      181 accgcgcccg cttctacaaa tatttcggta aggccctctt taatgcattg caggtaaacc
      241 ttttgtgttg ccggaattac tataacgcga acgccgtcct ttacctttct gccctttaag
      301 acggaggcgg cagcgcgcat atcctctatt cttccgtttg tgcaggagcc tataactacc
      361 tgatctatgt cgattttttc aatttcgtca acggtgtgag tgttttcggg aagatgcgga
      421 aaagcaaccg tgggtttcag attatttaag tctatctcta ttgtctcgtc atactcggcg
      481 tcctcgtctg cctcgaatat acgaggctgt ctgtcggtcc tgccgcttat gtattcgagc
      541 gtcctgtcgt ctaccgggaa aattccgttt ttggcgccgc attctattgc catattcgct
      601 atgcagaagc ggtcgtccat tgagagtgcg cctacaccgt cgcccgtgaa ttcaagcgaa
      661 cggtatcttg ctccgtcaac gcctatttta ccgataaggt gaagtattac atccttgccg
      721 ctgacaaatt cgggcaaggt gccttttagt acaaccttaa ttgcggacgg aaccttaaac
      781 caagccttgc cggagatcat accggcagcc atatcggttg agcccacacc cgtagagaac
      841 gcgccgagtg cgccgtatgt gcaggtgtgt gagtcggcgc ctataacggc gtcgccggaa
      901 ataacgagac cgctctcggg aagaagcgcg tgctctatac ccattttccc cacgtcaaag
      961 aaatttttga ttcccttttc ctttgcgaaa agacgaacct gtttgacatt ttcggcggat
     1021 ttaatatcct tgttgggggt aaaatggtcc ataacaagtg ctattttgtc tgttgaaaat
     1081 acgtccttaa agcccctttt tgccatctcg tttatggcaa cgggcgaggt tatgtcgttg
     1141 ccgagcacga ggtcgagctt tgcttctatg agctgacctg ccgtaaccga ctgaaggccg
     1201 gcgtgcgccg cgagtatttt ttgggtcatt gtcattgaca ttatgttatt cctcgattcc
     1261 ttttatgttt gtatcgccgc tctcaagcgc ggtaagaccg gagcgtgaaa gctcgattat
     1321 tccgtgcttt gtcattttgc ctatgaattt gtcgataagc gacggcagac ccgtaagctc
     1381 ggcagttatt gttgtgtggc ctatatcctt tacctttgcg ccgaagccgt tgattgtttt
     1441 aagcacggct gtgcgctggc tgtttgtggc gtggaccttt ataagcaaga gctcggagga
     1501 tacaaggcgc gattcattga gctttaccac ctttattacg tcctcaagct ttgagagctg
     1561 acgctcaacc tgcaaaaatt tgtgcggctc aacgtcggat actattgtta ttcttgaata
     1621 tacgggattt tccgtttcgg ctacggtaag gcttattacg ttgtagccgc gtctcgcaaa
     1681 aagacttgcg acgcgtaaaa gcacgcccgt gcggttatat acgagagccg agaggaatat
     1741 tctctcaaga ctgtttgcat tattttcttc tgccattttt gttttccttt cctttgcggc
     1801 ttattcggct gaaaaacgat acaccgtgtc agatacgaac ggatggtttt cgtcaaacag
     1861 attttgtatg aaatcggggt tgtttaaagc gttcgggtag tactgcgttt caaggcagaa
     1921 accggagcgg tgagtgaggg gcagcttacc tttaccgatt tgcgagccgt ccatatagtt
     1981 gcctatgtaa aactggactc cgggaaggtc ggtatagcac gtcatttttc tgccgctttc
     2041 ggggtcgtaa accattgcga attcgcgcag agcacccgtg taattttcga ggcagaagtt
     2101 gtggtcgtag cccctgccta ttatgagctg ctcgtcggga gacgatacgt ctctgcctat
     2161 ctttttcggt attctgaagt caaacggtgt gttttcaacg tttctgattt cgcctgtcgg
     2221 gatgctgtct tcgttcatcg gcgtaaagta ttttgcaaaa atctgcattt cgtgagagag
     2281 aatgttgccg gaagcgtatc cgccgaggtt aaagtatgag tggtttgtga cgtttatcgg
     2341 agtcttgccg tcgggtctgc agtcatagtg tattttgacc tcgtttttgt cggtaaacgt
     2401 gaaggtgact tcattttgaa ggtttgacgg gaagccctcg ttgccgtcgg cgctgaaata
     2461 tgagaattta acgctgtcgt cgccgagtat ttcataattc caaacggcgt tttcgtattc
     2521 gtgattaccg tgaagggtga atttaccgtc gatgtttttt gttatcgggt aatttttgcc
     2581 gtctatggta ataccgtttc cggaaattcg gtttgcgact cttccgacgg tgcgaccctg
     2641 cgaatccgta cagctcagct gaccctccat agtgtcaaag ccgacaagca catcggcaaa
     2701 gtctccgttt ctgtcgggaa ccgatatttt attgagcgta gcgccgtaat tcagcagttc
     2761 gatttcggct ccgctttcgt tttttattgt gaatgaaaag acttcttttc cgtcggcggt
     2821 tttgccgtag agagatttgg tcattttcaa agctaccacc ccatttttag cattttaata
     2881 agtataatat ccgtgcgcgc cgttgtcaag aaagacgaaa aaacttgcaa gagcgaaaat
     2941 aatgctgacc ggaaaacaaa agtattttta ttgacttttc ggcgttttaa atgtattctt
     3001 aacatatatt atgtatttga agggagtgtg ccgattggag caatttttaa ctcagtttga
     3061 attcctgtcg cgatatgctt tggcttttct tgcgcttata atacttctcc ggtgcatttt
     3121 ttcccttttc aggctcagac cgcgccgcga gattatggca acgctgataa atcttgccga
     3181 cgacagcgaa atcgagatac gaaattggga aacctcgata ggccgttcgc gctcgtgcga
     3241 tatatcgctc agaaagtacg gcacggtatc acgttttcac gccgttctgg ctctcagaaa
     3301 agacggctgg tatgtctttg ataccgaatc gaagacagga gtttacgtaa acggcgagca
     3361 gataaaaaaa tcagccaaga taatcgacgg cgacaccgtc tcgttcggaa atgcggttat
     3421 gaagttcacc tcaaacggct acggcgttga gagtgaggag gctgaggacg gtaaaaaggt
     3481 tgacttcaca aacgccacaa ggcttgtaaa tcttgccgac aacagcgcgt tctatttaaa
     3541 tggcacatgc cttatagggc gtgacagaac ctgtaatata tgcctgccga tgcaaagcgt
     3601 ctcgggcgag cacgccgagg tctatcccgc aaaggacggc tggtatgttg cggacctcaa
     3661 ttccaaaaac ggcacgcttt taaacggcga agaggttttc ggcgcattcc cgctgtatga
     3721 cggcgacatt atttcaatag gcgattacag cttccttttc cgcgaataaa gcggagaaga
     3781 ggccatattc aaagaaacgg aggacgcatc tttggctcag gaaaatgctc gtaaaaagca
     3841 aaaaaagacg agaaccaaaa aatacagaaa gcctgtaaac tggctgattc tgcttataat
     3901 tatgactgtt tttcagctcg tttgcgccgc tcagataaat atgaacagtc ccgacatacc
     3961 gctgactaca ttatatattg tattcggtgc atatatagct attgaatggg cgtattttat
     4021 atttttcggc ggatttttac accgcaagag ctttgaaata gagcttatag cgttttttct
     4081 taccggaata ggccttacac tcaccacaag cgtgtatccc gacaaagcgt ttgttcagct
     4141 tgcggcggtg attatgggta ttgcggtatt tatagttatg ctttgggtgc tcagcgatat
     4201 agaaagagtt gtaaagctgc gtatgccggt ggcgatagct tccgttatcg tacttgttgc
     4261 cacaaggctt ttggcgcaca atataaacgg tgcatacaac tggataagaa taggcggcgt
     4321 ttcaatacag ccgtcggaaa tagtgaagat aggtttcgtc tttgtaggtg cggctacgct
     4381 cgaatacctg caatcaacga gaagccttac aaaatatctt gtgtttgcgc tttcctgtat
     4441 aggctgtctg ttccttatga aggatttcgg tacggcactt atattcttct ttacattctt
     4501 aataatggca ttcctgcgct cgggagatat aaagacaata tttcttgtgt gcgcggcagc
     4561 tctcggcggc ggcggtcttg tgcttacgtt taagccgtat gtcgcaaaac gctttgaaac
     4621 atatcgccat gtttgggagt atgcaaacga ccgcggctat cagcaggcga gacttctcat
     4681 atattccgtc agcggcggtc ttttcggact cggaatagga aacggcaagc tgagagatgt
     4741 atacgcagcc acagaggact tggcgttcgg aatggtatgt gaggaattcg gaattattgt
     4801 cgcatttgcc gtgcttttga cctttgtcgc cctcgtggtt tacagtatac gctatgctgt
     4861 ggcggctcga tcgtcttttt acgctattgc tgcgtgcgct gcgtcgggtc ttcttctttt
     4921 ccaggcggct ctgcacgtgt tcggcgttac cgatcttctt ccgtggacgg gcgttacgct
     4981 gccgtttatc agccgaggcg gctcgagtat gatgtgctgc tggggacttg tggcatttat
     5041 aaaggccatt gatgtcaaga cgtacaaacg gtacgataag gtagcggtgg caaagtctta
     5101 aattaaatct gccgaaaggg agtgtgaaac aatgaaaacc atgtcaaaaa gagcctgggt
     5161 gccctatgtg tttatagcag catttttagc agggctcatt gtgttgtttt acacatttta
     5221 cacgaacgcc gattcgtggg ctatgaaaaa ggcaaacagg cacctgtatt catcaggtac
     5281 gctgtcggtt gcgggcagca ttaaggatga aaccggagcg gtgcttgcgg aaacaaagga
     5341 cggtaagcgc gtttataaca gcgacaaaac cgtaagaacc gcaaccatgc acacggtagg
     5401 cgactctgag ggctatatag cgacgggcgt tcagacatcg tataaggacg tcataaccgg
     5461 atataatttc gttgacggta tatacaacct gaaaaaatac ggtaagggca acgacgtaaa
     5521 tctcacaata aatgcgcagc tctgtgcggc ggcatacagc gccctcggaa gcaacaaagg
     5581 cacggtcggc gtttacaatt acaaaaccgg tcagctttta tgcgttgtat caagcccgtc
     5641 ttatgatatt gcaaacaagc ccgtctcaag tataaataac gataagacag gtgcctacga
     5701 gggtgtttat ttaaacaggt ttttctccgg cgtttatact cccggctcga catttaaaat
     5761 gataacctcg atttgtgcca ttgaaaattt gccggatata gattcgcaaa aattcaaatg
     5821 cacgggcgaa tacaaggtcg gcggcagctc ggttaagtgc ctcggggttc acggaaccgt
     5881 gaatttcgaa aaggctctca atgtgtcctg taacagcgca tttgcgcaga ttgcgataga
     5941 actcggaccc gataaactta tggccacggc aaaggagctg gggctcggca atcagatgta
     6001 tgtaggcaat ataaggctcg caaaaagttc gttcaacgtg accggcgcgt cggacctcga
     6061 cgtgggctgg gcaggtatag gtcagtacac cactcgcata aatccgtgcc agatgttaac
     6121 tattgcaggc gcggtggcta acggcggctc ctcagtgccg ccatatcttg tcggcaaaat
     6181 aacctcgcag accggaaaaa cgatttatga agcaaagccc ggcacggcaa ctcagcttat
     6241 ttcctcggat accgcatcaa agatgaacga tatgcttcgc tcaaatgtca aaaattatta
     6301 cagcgacggt aagttcccca accttaaaat gtgcggtaag accggaacgg cggaagtaag
     6361 ctcggaagcc ggcggtgcaa agcctcacgc ttggtttgtc ggatattcac agcgcgagga
     6421 tctcccgctt gcgatagtgg ttgttgtaga aaacggcggc ggaggctcat ctgtggcaat
     6481 tcccgttgcc aataagacta tgcaaaaggc tctttcctta tatgcaaaat aaaaattttt
     6541 ccgcatacga cagcaaagct cgtgtgcgga atttttaaag gtgataaaat gaaattttac
     6601 tttccggaaa actacggcgc tctcggcgac ggaaaaaacg acgattcacg cgctttacag
     6661 tccgctattg acgatgccga aaaaaacggc ggcggaacgg ttgtgcttga gagcggaaaa
     6721 acgtattatt cannnnnnnn nnattattca agctcggtca taatgaagaa aaacgttgag
     6781 ttgcatttgc aaaaaggctc ggttttaaag gcaacctcag acataaacgg atactttcgt
     6841 ccctgcgatt ttataaacga tgagacaaat acccttatag gtaatcccgt tacgggtaag
     6901 ccgtcgtttg cctttatata tgcgtataag gcggacaatg ccgcaataac cggaggcgga
     6961 aaaatcgacg caaacggtca cagctttgta aagagaaaag acaaatacta tgttaccggc
     7021 gacttttatc cgcgtcccac ggttatgtat tttgaggctt gcaaccatat tgtgtttgag
     7081 gatttcacgg tggtcgatgc accgttttgg acgcttcatc cggcaggctg cgacgatgtt
     7141 ttaatatcaa aaattcacat attaaacgac ctcgatgttg caaattcgga cggcatagat
     7201 cccgaccact gcagcaatgt gcgtatttgc gactgccacg tagagtgcgc cgacgactgc
     7261 atctgcctta aaacttcaaa aggcaattcc gaatacggac cgtgcgaaaa catactgata
     7321 acgggctgca cgctgatttc aacctcggcg gcgataaaaa taggcacgga gggcgtcggc
     7381 gatttcagaa acataatagt ttcaaactgt gtcatttcgc gcagcaaccg cggtctttca
     7441 attcagatac gcgacggcgg caatgtcgaa aatgtcaaat attccgacat tataataggt
     7501 acgcgccgct tttgccccga ttggtggggg actgccgagc cgatagtcat aaccgcgttt
     7561 gaccgcgacg aaaacacaag ggcaggccat atcaaaaaca tatcttaccg caacataacc
     7621 tgcgtcggcg aaaacggagt tatgatatgc ggtacggtgg aaaacaaaat tgaaaacgta
     7681 gttttctcgg atgttgacgt aaccctctca aaaacgtcaa agtgggactg cggtctttac
     7741 gatatgcgcc cggggcttaa taaagaggtc gaaaagcata aaaatgccgg tttttatctt
     7801 cgctttgccg ataatgttac gcttcgcaac acaagcgtga agtggggaaa tgtctgcccc
     7861 gaatattcgg ctgctctcga agaggaaagc tgtgtcggca ccgtacttga aaatttcacg
     7921 ggtgataacg cctgaatgct ttgagaatgc ggctgctcgt tcgagcgtga aatttccttt
     7981 caaacggctt cttgtatgta cgtttataag cattttactt aaaaacattt gacatagcgc
     8041 gtataaaatg gtacaattca acaatggagt taagctatcg cctgcttcgg caagcgagaa
     8101 tcgaaaggga gtatactgta atgaaaaagg cgttaaataa aaaagtgctt tccgtactga
     8161 ttgcggctgt tattttactt gttaccgcat ttcccgtgct tgcggcggat ccgtttaagc
     8221 tcacttcttc ggctgaagca aaggattcgg tcgtcaccgt aaaaatttac cttccgcaaa
     8281 atacggaact cgggacgctt tcgtttacac ttaaatacga tacaaaattg ctcacgtatg
     8341 tttccgccga gtattcgagc ggagatgtca gcgccacaaa ttcaagcacg gcaggcgagg
     8401 ttaaaataaa cgccgtttgg aaaaacaccg cgccgctcgg cggcgaggtt gccgccataa
     8461 cctttaaggt gaacgacgga gcgaccggca aaaccgactt cccgataaat gtcgatttct
     8521 caacagacgc caataatcag cacattgatt tgtcggccgt aagctcttcg attgacttat
     8581 ccgaagcagc tccgatgccg gcggcaaccg atgaaaccgt ctcatcggag ggcaaaacgg
     8641 taaaggtgga catcgaccag caccgccaga agatgggcat ggtgttccag cact
//
LOCUS       DBMDVO010000003         7031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_4046_length_7031_cov_1.274026, whole genome shotgun sequence.
ACCESSION   DBMDVO010000003 DBMDVO010000000
VERSION     DBMDVO010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4046_length_7031_cov_1.274026"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="ACZPE2_00095"
     CDS             <1..286
                     /locus_tag="ACZPE2_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1318279.1"
                     /translation="HKGSMIAVQGSIQTRSYEDKNGNKRTAVEIVADNVSFCGSKSEQ
                     GGGNRYEAQQPAAPSYQSGDSGSFSVLPSNDDNDGMPFGGASDEDDGLPF"
     gene            326..583
                     /gene="rpsR"
                     /locus_tag="ACZPE2_00100"
     CDS             326..583
                     /gene="rpsR"
                     /locus_tag="ACZPE2_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1318280.1"
                     /translation="MANERTAPQAGGRPMNRKHRKVCAFCVDKVEEIDYKDTAKLRRF
                     TSDRAKILPRRVTGTCAYHQRELTKAIKRARHIALLPYTSD"
     gene            complement(653..2239)
                     /locus_tag="ACZPE2_00105"
     CDS             complement(653..2239)
                     /locus_tag="ACZPE2_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1318281.1"
                     /translation="MSTKSKELPRAASPEEVGVSSALTEQFLHYIKEQNLEFHSFMVI
                     RHGKIAVEWYNEPYTADTSHSMYSVSKTFSATAIGFAVSEGLLSLDDKVLDFFPEYTP
                     KSDSPYFDKLTVRSLITMTSGKQTNMLEEKGKIDWIEDFINSPWVFEPGTQYLYVNEN
                     IYMLCAIIHRVAKTSVVDYLMPRLFEPLRIERPFWETDQNGIEAGGWGLYIKTMDLAK
                     VMTCYLHEGKYKNKQVLPKEWVKEATVNQIGDIKMPSKDKDCCAGYGYCIWMDDTEPY
                     SYRADGMFSQFGINFPSLDATIISTAAIPCEDEARAAIWAFFPAAFADENGNGVEVDT
                     SSVNRPVASKHSVTESRLIGKTIKVRKKILLNIIGMPVSMLPLAVTFMMSDRAGNIDN
                     IKFNFGDHECDMTWDEGDERNTVCCGMDGRYRYGTMTLGKIKFKVCANAEWIDDINLK
                     VMVRPVETVGMRTLNFLFRRNNKVTITPTSTPSTYKIVDTLSRSFTEIIKNPLLSKIC
                     QKAIQIAPPLAEPKHYGKIV"
     gene            complement(2433..4328)
                     /locus_tag="ACZPE2_00110"
     CDS             complement(2433..4328)
                     /locus_tag="ACZPE2_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1318282.1"
                     /translation="MKLKPQRHKANYKSLALILADIVIVNLAYPIMVLIYWNFRYSPD
                     LMLKLLIRIPFVTLFYIGSYAVSKLYRSVWKYAGIYELFRYAAATAAGTILVWLADYI
                     GMKICRHAGSEILYFNVLPWSIYLDCSLIIICACLAVRLAPRSFKVASAESSRAKRSN
                     TRILILGAGVAGSSLVHEFQQNGYRFGNPVVLVDDDPEKIGRSINGTPIEGGCDRLSQ
                     IAQKYNADEIIFCIKNADEKRKRELLEVAVSTGCKVKIVPDINNLPDGGKANYKEIRN
                     VDILDLLSRPEIKLDPETCKYLTGETVLVTGGGGSIGSELCRQIAKYAPKKIIIFDIY
                     ENNAYTLKQALDRHYFGSPEIIIRIGSVRDKARLREVFEEFHPSSVFHAAAHKHVPLM
                     EDSPFEAIKNNTLGTYNTAECANEYKVSHFVLLSTDKAVNPTNVMGATKRTCELIIQH
                     FAKISKGTTFAAVRFGNVLGSNGSVIPLFKEQLENGGPLTVTHPDITRYFMTIPEAAQ
                     LVVQAGGLAKGGEIFVLNMGEPVKILSLAEKLIKLSGFEPYVDIDIQFTGLRPGEKLY
                     EELVLQSEKDDIHKTQNDKIYVTSPGDIDDEKLISHIEKIRSMKPGDSREFLMELVPT
                     YTPDREA"
     gene            4652..6694
                     /gene="recJ"
                     /locus_tag="ACZPE2_00115"
     CDS             4652..6694
                     /gene="recJ"
                     /locus_tag="ACZPE2_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1318283.1"
                     /translation="MSRKKWTVSQYDKDMAAELAESYELDPFAALLLVSRGVTADNAD
                     EFLYPQCDIDPFLLPDMEKAANRIRKAIESFEKIAVFGDYDADGVTSAAVMYSYLESR
                     GADVICHIPDRINEGYGISPQAVENLKKRGVSLIITVDNGISAFDAAKTAKELSVDLV
                     ITDHHKQSGELPEAVAVVDPQRTDCNIPFRDWAGVGVAFKTICAVEGDGEEELLDEFS
                     DLVAIGTLADVVPLKKENRALVYEGLKRINSGSRQGIEALKNAAGVSGKKLGAGGISF
                     TLAPRINAAGRMGSAMTAFRLLLSDDENDAAELAKTIDTYNKERHSVENEITKQAIAE
                     IESRPDEKYASVIVVSGENWHQGVIGIVAARLCGKYGKPAVVISVDGEKGKGSCRSIE
                     GFSIYDALSAVSDTLTHFGGHTLAAGLGVEKSRIDEFRTAINEYAKTVEMPFPELRLD
                     CKLNPAYINMDLINSLELLEPFGAGNEEPCFGLFNMKISRITPIGDGKHLRLALKKGN
                     TEITALLFSVRAEEFPFRLGDTVDAAVKITKNEFRGEVKPSVRICDMRFSGSNDVNYL
                     KSVRLYEKYRRHEKLNEKELRFITPNRDFLASAYKFFKKSGGWHFDLHSLLIRANCPE
                     ASAATLLVSEDVLCELKLMEKTENGVFSLCETSEKKDLDSSKILAGLKAEMGENHG"
     gene            6687..>7031
                     /locus_tag="ACZPE2_00120"
     CDS             6687..>7031
                     /locus_tag="ACZPE2_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1318284.1"
                     /translation="MADIEKTKATEAEKTEYAEPDMRPEKLIKQQEREAKMTPDELYA
                     ELKARLDKAFTAEEAAEIDKAFRVAREAHKAQLRYSGQPYIIHPIAVSNILLDLGMDA
                     QSVEAALLHDTVE"
BASE COUNT         1853 a         1593 c         1709 g         1876 t
ORIGIN      
        1 ccacaagggc tctatgatag cagttcaggg ctctattcag acccgttcct atgaagataa
       61 gaacggcaat aagagaaccg cagtcgagat agttgccgat aacgtatctt tctgcggcag
      121 caagtccgag cagggcggcg gcaatcgcta tgaagcgcag cagccggcag ctccgtctta
      181 tcagagcggc gactcgggca gcttttcggt actcccgtca aatgacgaca acgacggtat
      241 gcccttcggc ggcgcgtcgg acgaggatga cggtttaccg ttctaaacat tgaatttttc
      301 tacattttaa aaggaggata ccataatggc taacgaaaga acagctcccc aggctggcgg
      361 ccgtcctatg aacagaaagc acagaaaagt ttgcgctttc tgcgttgata aggtcgaaga
      421 aatagattac aaagatacag cgaaacttcg ccgttttact tctgacagag caaagatttt
      481 gccccgcaga gtaaccggta cctgcgctta tcatcagaga gaactcacta aggcaataaa
      541 gagagcacgt cacattgctc ttcttcctta cacttcagac tgattttctt tttaatcaat
      601 caaaaacgcc cgttccgaga taccggaacg ggcgtttgtt ttgcctgtat tttcagacaa
      661 ttttaccgta atgcttcggc tccgcaagcg gaggcgcaat ttgtatggct ttttgacata
      721 tctttgagag cagcggattt ttgataattt ccgtgaaact ccttgagagc gtgtcaacta
      781 ttttgtatgt tgacggtgtc gatgtcggag ttatggttac cttgttgttg cgacggaaaa
      841 ggaagttgag tgttctcata ccaaccgttt ccaccggacg aaccataacc ttgaggttaa
      901 tgtcgtcaat ccactcggcg ttggcacaga ccttgaattt gatttttccg agcgtcatcg
      961 tgccgtatcg gtaacgtccg tccataccgc agcaaacggt atttctctcg tcgccctcgt
     1021 cccatgtcat atcacattcg tgatcgccga aattaaactt gatattgtca atgtttccgg
     1081 ctctgtcgga catcataaac gtaacggcaa gcggaagcat actcacgggc atacctataa
     1141 tgttgagcag tatctttttg cgaactttta ttgtcttgcc tataaggcgg ctctcggtta
     1201 cggagtgctt tgaagccacg gggcggttga cgctcgacgt atcaacctca acgccgttgc
     1261 cgttttcgtc ggcgaatgct gcggggaaga atgcccatat tgcggctctt gcctcgtcct
     1321 cgcacgggat ggcggcggtt gaaattattg ttgcgtcaag agaagggaaa tttatgccaa
     1381 actgagagaa cataccgtcg gcacggtatg aataaggctc cgtatcatcc atccaaatgc
     1441 agtaaccgta gccggcacag caatccttat ccttgctcgg cattttgatg tcgcctattt
     1501 ggtttacggt agcttcttta acccattcct tcggcagaac ctgcttattt ttatatttcc
     1561 cttcgtgtag ataacaggtc attacctttg cgaggtccat ggttttgatg taaagacccc
     1621 atccgcctgc ctctatgccg ttttggtcag tctcccaaaa cggtctttca attctgagcg
     1681 gctcaaaaag tcgcggcata aggtagtcta ccacgctcgt ttttgccacg cggtggataa
     1741 tagcgcagag catataaata ttctcgttga catagaggta ttgcgttccg ggctcaaata
     1801 cccatggcga atttataaaa tcctcaatcc aatcgatttt tcctttttcc tcaagcatat
     1861 tagtctgctt accggaggtc atggttataa gactccgtac cgtcagcttg tcaaagtacg
     1921 ggctgtccga tttcggagtg tattcgggga aaaaatcaag aaccttgtca tcaagtgaca
     1981 gaagcccttc agataccgca aagcctatcg cggttgccga aaaggtttta cttacggaat
     2041 acatggaatg gcttgtatcg gccgtatagg gctcgttata ccactcgacg gcaatcttgc
     2101 cgtgccttat aaccataaat gagtggaatt caagattttg ctcttttatg tagtgtaaaa
     2161 actgctccgt caatgccgat gaaacgccga cctcttcggg agacgcggcg cgcggaagct
     2221 ctttgctctt tgtactcaag cgacacaaca cctttcaatt aacaatatag agacattgta
     2281 tcacttcaac aaatattttg caactaaaat aaaaaaacta tacaataaag cagatattat
     2341 gcacatatta cacagctgtg ttcattataa aaagtgagca ggtctttctt ctcggaacga
     2401 cttgctcact tttgtttgaa tatttgattt tctcaagctt cacgatcggg cgtgtatgta
     2461 ggaacaagct ccatgagaaa ttcgcgggaa tcacccggct tcatcgaacg tattttttct
     2521 atatgggaaa tcagcttttc gtcatctata tctcccggag atgtgacata aattttgtca
     2581 ttttgagtct tgtgtatatc gtccttttcg gattgaagaa caagctcctc gtagagcttt
     2641 tcgccgggac gaagccctgt aaactgtatg tcaatatcaa catacggctc aaaccccgaa
     2701 agctttataa gcttttcggc aagcgagagt atctttaccg gctctcccat attaagcacg
     2761 aatatctctc cgccctttgc aagtccgccg gcctgaacaa caagctgtgc tgcctccggt
     2821 atagtcataa agtaacgggt tatgtcggga tgcgttacag taagcggtcc gccgttttca
     2881 agctgttctt tgaaaagagg tattacgctg ccgtttgaac cgagtacatt accgaagcgc
     2941 acggcggcaa aggtggtacc ctttgatatt ttggcgaagt gctgaattat aagctcgcac
     3001 gtcctcttgg ttgcacccat gacatttgtc gggttgaccg ccttgtccgt cgagagaagc
     3061 acgaagtggc tgaccttgta ttcgttagca cattcggcgg tgttgtatgt gccgagtgtg
     3121 ttgttcttta ttgcctcaaa cgggctgtcc tccataagcg gaacgtgctt gtgggccgcc
     3181 gcgtgaaaaa cgcttgacgg gtgaaattcc tcgaatacct cacgaagtct cgccttgtcg
     3241 cgtacggagc ctatacggat gattatttcg gggcttccga aataatgccg gtccaaggcc
     3301 tgctttaatg tgtatgcgtt gttttcgtat atgtcaaata ttataatctt tttcggagca
     3361 tattttgcta tctgacggca cagctcggag cctatgcttc ctccgccgcc cgttaccaaa
     3421 accgtttctc ccgtgagata tttacatgtt tcggggtcaa gctttatttc gggacgcgat
     3481 aaaaggtcga gaatatcaac gtttctgatt tccttgtaat ttgccttgcc gccgtcgggg
     3541 aggttgttta tatcgggaac tatttttacc ttgcagcctg tggagacagc cacttcgagc
     3601 aattcacgtt ttcttttctc gtcagcgttt tttatgcaaa aaataatttc gtcggcgtta
     3661 tatttttggg cgatttgaga gagtctgtca catccgccct caataggtgt accgtttatt
     3721 gagcgcccta ttttttcggg gtcatcgtct acgagtacaa caggatttcc gaagcggtag
     3781 ccgttttgct gaaattcatg tacaagagag cttcccgcta cacctgcacc caaaatgagt
     3841 attcgtgtgt tagacctttt tgcgcgtgat gactcggcac ttgcgacttt gaatgaacgc
     3901 ggagcaagtc tcaccgcaag gcaggcgcag attattatga gcgagcagtc aaggtatatt
     3961 gaccacggca gtacgttgaa atataagatt tcgctccctg cgtggcgaca gattttcatg
     4021 cctatgtaat ctgccagcca tacaagaatt gtgcctgcgg cggttgccgc cgcatatctg
     4081 aacaattcgt atatcccggc gtatttccat accgaacggt aaagctttga tacggcgtaa
     4141 gaacctatgt aaaacagcgt tacaaacggg attcttatca gcagcttcag cataaggtcc
     4201 ggactgtatc tgaaattcca ataaatcagg accattatcg gataagcaag gttgactatt
     4261 actatatccg ccagaatgag cgccagcgac ttatagttgg ctttgtgcct ttgtggtttg
     4321 agtttcatta tttttctttt cccccaagaa aacaacttat gtacatttta cacttttatg
     4381 atagtagtgt caataatact atggtatcat ttgagtacat aattgatttt ataagctgtc
     4441 atttaaggct ttttatttgt aataaatcac cgctgaaaaa aatcaaccgt taaaatagta
     4501 ttgaaacaca taaataataa tgataaaata ttaaggttaa taattgtgcc gcaccgattt
     4561 gacagtttgc tcggataaag gcagtttaaa tgcctgtggg cttcggccgc gtaaaatcgt
     4621 taggcggtat ttatgtttgg agtaatatgt tttgagtcgt aaaaagtgga cggtatcaca
     4681 atatgataag gacatggcgg cggaattagc cgagagctat gagcttgacc cgtttgcggc
     4741 attgcttctc gtttcacgcg gagtaacggc ggataacgcc gatgagtttc tgtatccgca
     4801 gtgcgacata gacccttttt tgcttcccga tatggaaaag gcggcaaaca gaataagaaa
     4861 agcgatagag tcatttgaaa aaatcgcggt tttcggcgac tatgacgccg acggtgttac
     4921 ctctgccgcg gttatgtaca gctatcttga aagtcgcggt gccgatgtta tttgccacat
     4981 tcccgacaga ataaatgagg gctacggcat atcgccgcag gctgtcgaaa acttaaagaa
     5041 acgcggtgtt tcgcttataa tcaccgtcga taacggcatc tcggcttttg acgccgcgaa
     5101 gaccgcaaag gagctttcgg tggaccttgt tataacagac caccataagc agagcggaga
     5161 gcttcccgaa gctgttgccg tggttgaccc tcaaagaacg gactgcaata taccgtttcg
     5221 cgattgggcg ggtgtgggcg tagcatttaa aacgatttgc gccgtagagg gcgacggcga
     5281 agaagagctg cttgatgagt tttccgacct tgttgccata ggaacccttg ccgacgtcgt
     5341 tcccctgaaa aaagagaacc gcgctctcgt gtatgagggc ttgaagcgta taaattcggg
     5401 cagccgccaa ggaatagagg ctcttaaaaa tgcggcaggc gtttcgggca agaagctcgg
     5461 cgcaggcgga atatccttta ccttggctcc gcgcataaac gccgcaggca gaatgggctc
     5521 cgccatgact gcgtttcgcc ttttactgag tgatgacgag aatgatgccg cagagcttgc
     5581 aaaaacaata gacacttaca ataaagagcg tcacagcgtt gaaaacgaga taacaaagca
     5641 ggcaatagcg gaaatagaga gccgtcccga cgaaaaatat gcgagcgtca tagtggtgtc
     5701 gggtgaaaat tggcaccagg gcgtaatagg aatagttgcc gcaaggcttt gcggaaaata
     5761 cggaaaaccg gccgttgtca tttccgtgga cggtgagaaa ggcaaaggct cgtgccgcag
     5821 catagagggc ttttcgatat atgacgcact ctccgccgtt tccgacacat tgacccactt
     5881 cggcggtcat actctagccg caggtctcgg tgtcgaaaaa agcaggatag acgaatttcg
     5941 cacggcgata aacgagtatg caaaaactgt cgaaatgccg tttccggagc tcaggctcga
     6001 ttgcaaatta aatcctgcat acataaatat ggatttgata aacagcctcg aattgcttga
     6061 accgttcggc gcaggtaacg aagagccgtg cttcgggctg tttaatatga agatatccag
     6121 aataaccccg attggcgacg gcaagcactt gagacttgcg ttgaaaaagg gcaatacgga
     6181 gattacggct ttgctgtttt ccgtgcgggc ggaggaattt cccttcagac tcggcgatac
     6241 ggttgacgcg gcggttaaaa taacaaaaaa cgaatttcgc ggcgaagtaa agcccagcgt
     6301 tcgcatatgc gatatgcgct tttcgggcag taacgacgta aactatttga aatccgtaag
     6361 actgtacgaa aaatacagac ggcacgaaaa gctgaatgaa aaggaactta ggtttattac
     6421 accgaacaga gattttctcg cgtcggcata taaatttttt aaaaagagcg gaggctggca
     6481 ttttgacctt cacagccttc ttataagggc aaattgtccc gaggcttcgg cggctacgct
     6541 gctcgtttcc gaggacgtgt tgtgtgagct gaagcttatg gaaaaaacgg aaaacggagt
     6601 tttttcgttg tgcgagacaa gtgaaaagaa agaccttgac agctcaaaaa ttttagcagg
     6661 cctaaaggca gaaatggggg aaaaccatgg ctgatattga aaaaacaaaa gccaccgagg
     6721 cagagaaaac agaatatgca gagccggata tgcgccccga aaagctgata aagcagcagg
     6781 agcgagaagc aaaaatgacc cccgacgagc tgtatgcgga attaaaggct cgccttgata
     6841 aggcctttac cgccgaagag gcagccgaaa tcgacaaggc cttccgcgtg gcgcgcgagg
     6901 ctcataaggc acagctgaga tattcgggtc agccgtatat tattcaccct attgccgtgt
     6961 caaatatact tcttgacctc ggtatggacg cacaatcggt agaggcggcg cttttacacg
     7021 atacggtcga g
//
LOCUS       DBMDVO010000004         6395 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_4699_length_6395_cov_1.352717, whole genome shotgun sequence.
ACCESSION   DBMDVO010000004 DBMDVO010000000
VERSION     DBMDVO010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4699_length_6395_cov_1.352717"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(195..270)
                     /locus_tag="ACZPE2_00125"
     tRNA            complement(195..270)
                     /locus_tag="ACZPE2_00125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Ala,seq:cgc)
     gene            complement(440..2704)
                     /locus_tag="ACZPE2_00130"
     CDS             complement(440..2704)
                     /locus_tag="ACZPE2_00130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1318285.1"
                     /translation="MREVININKNWLFSKKEQPVPKTLPEDWESVNLPHTWNGTDGQD
                     GGNDYYRGKCCYVKLLKKADLGEKPVHYIQFDGVNSSAEVWWNGEKIGSHDGGYSAFR
                     VRIPEISDENILTVYADNSPNDTVYPQVADFTFYGGIYRDVTVIGVDESHFDLEFYGS
                     SGIMITPKVSGLSAAVNITARVTNPQDCSVRFVVTDADKKPVGEKSVDASDGKTVIEI
                     ENAHLWNGTQDPYLYSLTAELLKDGEKTDEISVRFGCRSFSIDPQKGFILNGKSCPLR
                     GVSRHQDRPGIGNALTEKEHREDMDLICELGANTIRLAHYQHSRVFYDLCDERGMAVW
                     AEIPYISRHMPGGRENTVSQMKELIYQNINHPSIIVWGLSNEITMNGASDSSLIENHR
                     MLNDLVHKIDPTRPTTIAVLSMCDPGEEYVRIPDVLSYNHYFGWYGGKTDMYGPWFDK
                     FHKKYPDRAVGMSEYGCEALNWHTSDPQQGDYTEEYQAKYHEDVIRQIAVRPWLWSTH
                     VWNMFDFAADARSEGGENGMNHKGLVTFDRKYKKDSFYAYKAWLSDEPFVHICGKRYI
                     DRPENVTSVTVYTNEPSVELFANGKSLGVQKRGEFPFFYFSVPNEGETVLTAKAGDCT
                     DESRIRKVDKANPDYVLQEEGAVINWFEIETPPGYMSVNDTIGDILATAKGKLLALKI
                     LKMVRANMKKNKGKSTGGMADMAKGMKINKSIIEMGKGFSVKRVCMMAGGLFTKKQIL
                     EINASLNKIKKKQK"
     gene            complement(2731..4119)
                     /locus_tag="ACZPE2_00135"
     CDS             complement(2731..4119)
                     /locus_tag="ACZPE2_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318286.1"
                     /translation="MDNGKTLRPFGMRDKIGYAFGDFGCNMSFAFINSYLMLFYVTCM
                     KINPKHFAILILLGKIFDAINDPIIGGLCDATKPGKDGKFKPWIKWASPVLLLSSIAL
                     FIYAPNAPYGVKIAMCLGIYCLWSVAYTSVNVPYGSMQSCITNDPDGRSALSTWRSIG
                     AMCAQIPIMILLPLLVYDKNDNPRGELFIVFVGVMGAIGLVSFWLLRRMTTERLAPAV
                     ASEKQKFNYFATLKSFFKNKPMMGATISTVAYLALMMTVTTSMPYVFMCYFKNTKLIT
                     IASMLAGCPIALGIVLAKPMLKKFSKKQLCTYPFLLSALSAGIAAFVRIKNPYVWIAL
                     VAVSMFGAAFYLTLMWALVADCIDYQEKKTGRREESSIYATYSLFRKIAQGVGAAVVS
                     YAIGATGYNQNLGALEQAPGVPEKIYFVTAFLPFIGAIISFLSMLFLYTLDDNAQENG
                     KDGKDKKEKTQE"
     assembly_gap    4249..4258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4365..>4684)
                     /locus_tag="ACZPE2_00140"
     CDS             complement(4365..>4684)
                     /locus_tag="ACZPE2_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318287.1"
                     /translation="SALLSCYLGYDYKNSPYYPYLQPEEIIDKSDIPPAYIVTSVKDF
                     LHSASDDLDKLLTEKGKEHMYHDWQLTVNRSSGHITSVAYPDLPESAETIDEMLAFFE
                     AHS"
     assembly_gap    4685..4694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4695..5671)
                     /locus_tag="ACZPE2_00145"
     CDS             complement(<4695..5671)
                     /locus_tag="ACZPE2_00145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1318288.1"
                     /translation="MTNVYMKDSLKRITAVILLAAATLLVALQCGSIAKKEEAAALLR
                     ADASSSCNKMPPDGFFRESVESPKAAVDDKINASENAGIDVKNELTALFTDLYGLVGD
                     VLSGNDVDTGELEVRFKNIFDGLFKLDADTVKTDPTADGVLKNDGFVASAAEVLWNYM
                     DYYDTNRLKKQSESAGVTVKKDIPYISDGNKYHLLDIYYPENAAEKLPVIIDIHGGGL
                     MYAEKEVNRVYASRLAKRGYIVVCINYSLCPDVTYPTQVSDVMASYRWVAENGEAYGF
                     DLDRVFVAGDSAGGQLAFYTAAVNTSDELKSLYGVSDTGLNIKAIGLISG"
     gene            complement(5779..>6395)
                     /locus_tag="ACZPE2_00150"
     CDS             complement(5779..>6395)
                     /locus_tag="ACZPE2_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1318289.1"
                     /translation="ADVLRTYVLFMGDYEQAAPWSESSVKGCKRFIDRVFNLQEILTD
                     GDEYSKELMSAMHKTVKKVSEDIEQMKYNTAIAALMTLLNKIYEIGKINRAELKTLII
                     LVNPFAPHVTEEMWANCGYGEMLAKDAKWPSFDEAKCVDSTVEIVVQINGKIRARLSV
                     PTDIESDKAIALAKNDEGIAAALEGKNIIKEIYVKGKLVNIVAK"
BASE COUNT         1571 a         1606 c         1424 g         1774 t
ORIGIN      
        1 tcaattggtc ggatcggaat taattatacc accgaattat gaaaaattct accgccatgt
       61 tcagtgtggc aggatcactc ttccgcagaa gtactctatg atggggtttg ttataagcct
      121 gaatgccttg tttcaagcgg ttttggggcg tagaaaaagt ccgccgcaat tctattgaaa
      181 ttacggtgga cttatggtgg acctgaagag attcgaactc tcgacttcta caatgcgaat
      241 gtagcactct cccagctgag ctacaggccc gtgaacccaa ttattataac acgcgacggg
      301 tagtagggca agagcttttt gaaatttctc gcattaaaaa tgctgtttta aattttacgc
      361 cgcaaagcag actcctttat cggcagtttg acaagccgac aaagtcccgt gcaaagtgat
      421 tttgcacggg acagcttttt tatttctgct ttttcttaat cttgttaagg cttgcgttga
      481 tttcaaggat ttgtttcttt gtaaacaagc cgcctgccat catacatacg cgcttgacgc
      541 tgaagccctt gcccatttct attatactct tgttgatttt catacccttt gccatatccg
      601 ccataccgcc tgtggacttg cccttgttct ttttcatatt ggcacgaacc attttgagaa
      661 ttttcaaagc gagcaatttg ccctttgcgg tagccaaaat atcgcctatg gtgtcgttta
      721 cgctcatata tcccggcgga gtctcgattt caaaccagtt gataaccgcg ccctcctcct
      781 gcaatacata gtcgggattc gccttatcga ccttgcgtat gcggctctcg tcggtgcagt
      841 cgcctgcctt tgccgtaagc acggtttcgc cctcgttcgg taccgagaag taaaagaacg
      901 gaaattcccc gcgcttttgc acgccgaggc tcttgccgtt tgcaaaaagc tcaacggacg
      961 gctcgtttgt ataaaccgtg acgctcgtca cgttttctgg gcggtcaata tagcgtttac
     1021 cgcagatgtg cacaaacggt tcatccgaca accacgcctt atatgcataa aaagagtcct
     1081 ttttatattt cctgtcgaac gtaacaaggc ccttgtgatt cattccgttc tcgccgccct
     1141 cgctgcgtgc gtctgcggca aagtcaaaca tattccagac gtgagttgac caaagccacg
     1201 ggcgaacggc aatctgacga attacatcct cgtgatattt tgcctgatat tcctcggtgt
     1261 aatcgccctg ctgcgggtcg gaggtatgcc aattaagagc ctcgcagccg tattcgctca
     1321 taccgacggc acggtcggga tattttttat ggaacttatc gaaccacggc ccgtacatat
     1381 cggtcttgcc gccgtaccaa ccgaaatagt gattgtaaga caaaacgtcg ggaatcctaa
     1441 catactcctc gcccgggtcg cacatactga gcaccgctat tgtggtgggg cgcgtcgggt
     1501 cgattttatg caccaaatca ttgagcatac ggtgattttc gataagcgac gaatcagacg
     1561 caccgttcat ggtaatttca tttgacaagc cccaaactat tatgctcgga tgatttatat
     1621 tctgataaat cagctccttc atctggctta cggtattttc tctgccgccc ggcatatgcc
     1681 gcgaaatgta cggtatctcg gcccaaaccg ccataccgcg ctcatcgcat aggtcataaa
     1741 agactctgct gtgctgataa tgtgcaagcc gtatggtatt agcacccaat tcgcatatca
     1801 aatccatatc ctcacggtgc tccttttcgg tgagcgcgtt gccgatacct ggtctgtcct
     1861 gatgcctcga aacaccgcgc agagggcaag acttaccgtt gaggataaat cccttttgcg
     1921 ggtctatgga aaagctgcgg cagccgaacc ttaccgaaat ttcgtctgtt ttttctccgt
     1981 ccttcaaaag ctccgctgta agagagtaaa gatacgggtc ctgagtgccg ttccacagat
     2041 gcgcgttttc gatttctata accgtctttc cgtcggacgc atcgacgctc ttttcgccca
     2101 cgggcttttt atccgcgtcc gtcacgacaa aacgaacgga gcagtcctgc ggatttgtga
     2161 ccctcgcggt tatattgaca gctgcggaca agccgcttac cttcggggtt atcataattc
     2221 ccgacgagcc gtaaaactca aggtcaaagt ggctttcatc gactccgata accgttacgt
     2281 cgcggtaaat tccgccgtaa aacgtaaagt cggcaacctg cggatacacc gtgtcgttcg
     2341 ggctgttatc ggcgtaaacc gtgagtatgt tttcgtccga aatttccgga atccttacac
     2401 ggaatgcaga atatccgccg tcgtgtgaac ctattttttc gccgttccac caaacctcgg
     2461 cactgctgtt tacaccgtca aactgaatgt agtgcacggg cttttcgccc aaatccgctt
     2521 ttttaagcag ctttacatag cagcatttgc ccctgtaata atcgtttccg ccgtcctgac
     2581 cgtcggtgcc gttccaggtg tgcggcaggt tgacgctctc ccaatcctcc ggcagagttt
     2641 taggcacagg ctgctctttt tttgaaaaaa gccaattttt gtttatattt attacttctc
     2701 gcatagcttt cctccgtgga aattataaaa tcactcttgt gttttttctt ttttatcttt
     2761 accgtctttg ccgttttcct gtgcgttatc gtcaagagta tacaaaaaca gcatactcaa
     2821 aaagctgatg atcgcaccga taaaaggaag aaatgcggtc acgaaatata ttttttcggg
     2881 aacgcccggt gcctgctcaa gcgcgccgag gttctgattg tagccggttg cgcctattgc
     2941 gtagcttact accgcggctc ctacgccctg cgcgatttta cggaaaagag aataggttgc
     3001 atatatggag ctttcctcac gacgccccgt ttttttctcc tgatagtcga tgcaatccgc
     3061 aacgagtgcc cacataagtg tgaggtaaaa cgccgcgccg aacattgaaa cggctacgag
     3121 ggctatccag acatagggat tttttattct tacaaacgcc gcaatccctg ccgaaagagc
     3181 cgacagtaaa aacgggtatg tgcaaagctg ctttttgctg aattttttaa gcatgggctt
     3241 tgcaaggact attccgagtg ctatggggca gcccgcaagc atggaggcaa tcgttataag
     3301 cttcgtgttt ttaaaatagc acataaacac gtacggcatt gatgtggtta cggtcatcat
     3361 aagtgctaag taagcgacgg tgcttatggt ggcacccatc atcggcttat tcttaaagaa
     3421 tgacttcaaa gtggcaaaat agttgaattt ctgcttttcc gaagcgacgg caggtgccaa
     3481 gcgttcggtt gtcatacgtc tgagcagcca gaatgaaaca agccctatgg ctcccataac
     3541 gcctacaaaa acgataaaca gctcacctct ggggttatcg tttttatcat aaaccaaaag
     3601 aggcagtaat atcatgatcg gaatctgagc acacatagcg ccgatactgc gccacgttga
     3661 cagcgcggaa cgaccgtcgg ggtcgtttgt gatgcagctc tgcatagagc cgtaaggcac
     3721 gtttacgctt gtgtaggcaa cgctccaaag gcagtatatg ccgaggcaca tagcaatttt
     3781 cactccgtac ggggcattcg gcgcatagat aaacaaagct atgcttgaaa gaagcagtac
     3841 ggggcttgcc cattttatcc acggcttaaa tttaccgtct tttcccggct ttgtcgcgtc
     3901 gcaaagaccg cctataattg ggtcgtttat cgcgtcgaaa attttaccga gcaaaatgag
     3961 aatcgcaaaa tgcttcgggt ttatcttcat acaagtaaca tagaacagca taagatagct
     4021 gtttatgaac gcaaagctca tattacagcc gaagtcaccg aaagcatagc cgattttgtc
     4081 gcgcatgccg aacgggcgca gggttttgcc gttgtccata agggacacct ccgaattctc
     4141 tgtgtctttc ggacaatgtg acttgcgccg agtcttttat gaattaccgt ccttcggtgc
     4201 aaagcgattt tgtccttatt ttgtataaca ctattatata tgatatccnn nnnnnnnnta
     4261 tccaaaaaaa atcaatataa agcagagacg ttaggctaat ttgtcagctt ttaaggcgaa
     4321 aaaaggaact gttttgggta aaagcccatt gcggttccgt ttgtttatga atgtgcttca
     4381 aaaaacgcga gcatttcatc aatcgtttcc gcgctttcgg gaagatcggg ataggcgacg
     4441 ctcgttatat gccccgacga acggttcacg gtaagctgcc aatcgtgata catatgctcc
     4501 ttgccctttt ccgtcaaaag cttatcgagg tcatccgaag cagagtgcaa aaagtccttg
     4561 acactcgtca caatatatgc cggcggaatg tcggatttat caattatctc ctcgggctgc
     4621 aaatacggat aatacggcga atttttataa tcgtagccga gatagcacga taaaagcgcg
     4681 ctgtnnnnnn nnnnccggaa atcaacccta tcgcttttat gttgagtccg gtatccgaaa
     4741 cgccgtaaag cgattttaat tcgtcgctcg tatttaccgc cgcggtgtaa aatgcaagct
     4801 gaccgccggc agaatcacct gcaacaaaga ctctgtcaag gtcaaagccg taagcctcgc
     4861 cgttttccgc cacccagcgg tacgacgcca taacatcgct cacctgcgtc gggtatgtta
     4921 cgtcgggaca cagagaataa tttatacaca caactatata tcctcttttc gcaagacgcg
     4981 acgcatagac gcgattaacc tctttttccg catacataag tccgccgccg tgtatgtcga
     5041 ttatcaccgg cagcttttct gcggcatttt cgggataata aatatcaaga agatgatatt
     5101 tgttgccgtc cgaaatataa ggtatatctt ttttcaccgt cacgcctgcg gattccgact
     5161 gttttttcag cctgtttgtg tcgtaataat ccatataatt ccacagcacc tcggcggcag
     5221 acgccacaaa gccgtcgttt ttaagcacgc cgtcggcggt tgggtcggtt tttacggtat
     5281 ccgcgtccaa tttaaacagc ccgtcaaata tatttttaaa tctgacttca agctcgcccg
     5341 tgtcaacatc gtttcccgac aaaacgtccc ccacaaggcc gtaaaggtcg gtaaacagcg
     5401 cagtaagttc atttttgacg tctataccgg cgttttcgga agcgttgatt ttatcgtcaa
     5461 cggcagcctt cggcgactcc acgctttcac gaaagaagcc gtcgggaggc attttattgc
     5521 acgaagagga agcgtcggca cgtaaaagtg ctgccgcttc ctcttttttt gctattgagc
     5581 cgcactgcaa agcgacaagc aaggttgccg ccgccagcag tataacggcc gtaattcttt
     5641 ttaaactgtc cttcatataa acatttgtca tttaccgagc gccgaaaaca gcgagcctat
     5701 aaacacaaag aagtccgcaa cgcgcgcttt tgtatttttg tgacagctta tccgcaaaaa
     5761 tcgaaaaacg atatatcatt atttagcgac aatattgaca agcttgccct ttacatagat
     5821 ttccttgatg atatttttac cctcaagagc ggcagctatg ccttcgtcat tctttgcgag
     5881 agctattgcc ttatcgcttt caatgtcggt gggaacagag agtctcgctc ttatcttacc
     5941 gtttatctgg actactattt caacggtgct gtcaacacat ttagcctcgt caaagctcgg
     6001 ccactttgca tcctttgcaa gcatttcgcc gtaaccgcaa ttcgcccaca tctcctcggt
     6061 gacgtgcggt gcaaacgggt tcacgagaat aataagcgtt ttgagctcgg cacggttgat
     6121 tttaccgatt tcgtaaattt tgttgagcag cgtcataaga gcggctattg ccgtgttata
     6181 cttcatctgc tctatgtcct cggaaacctt tttgacggtc ttgtgcatag cgctcataag
     6241 ctcttttgaa tactcgtcgc cgtccgtcag gatttcctga agattaaata ctctgtcgat
     6301 aaatctcttg cagcccttaa cgctcgattc gctccacgga gcagcctgct catagtcgcc
     6361 cataaacaga acgtaggtgc gcagtacatc ggcgc
//
LOCUS       DBMDVO010000005         6312 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_4797_length_6312_cov_1.050233, whole genome shotgun sequence.
ACCESSION   DBMDVO010000005 DBMDVO010000000
VERSION     DBMDVO010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4797_length_6312_cov_1.050233"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="ACZPE2_00155"
     CDS             complement(<1..253)
                     /locus_tag="ACZPE2_00155"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR1318290.1"
                     /translation="MNKKTVFKLSAAAICVLLSACMLFACGKKQKDFETDTSAPVTTA
                     EPAAATDVNPLTGLTGLPESSIGKRPVCVMVENSPEARPQ"
     gene            complement(384..2456)
                     /locus_tag="ACZPE2_00160"
     CDS             complement(384..2456)
                     /locus_tag="ACZPE2_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1318291.1"
                     /translation="MKAYTSNNIRNIAVLGHGGKGKTTLCEAMLYIAGASDRLGRVAD
                     GNTVLDFDSEEKRRKSSVSSAIAALEWDNTKLNIIDAPGLFDFEGGAAEAVRAAEAVL
                     IVTSAGSGVDVGTEKAFRAAEKKGAAKFFAVTKTDSDHRNFYKTFDALKEVYGNKLCP
                     TIIPYMEGNITKCYVNLMTGMAFSYDENGKATKVPVPEDSVIEEMTSAFMEAVATTDD
                     ALMEKFFEGEKFTKEEILKGLCIGIFDGSIYPVYAVSGLTCAGVDQLLYGLAWSAPGA
                     FGYAGEKCTYENGEEGILPCDENGPLAAVCFKTIADPFVGKMSFFKVVSGKLAPDTAA
                     YNFRTGEIEKMGKIVFVKGAKQEDAPAIIAGDIGVVTKMAGVKTGDTLCDPKNILKLA
                     GVDFPKPCLSMAVKVSKKGEEEKVAAGLTRLMEEDPTITFALNKETREQVLSGLGEQH
                     LDVIVSKLKNKFGVDVTLELPKVAYRETIRKPVEAQGKHKKQSGGHGQFGDVWIKFEP
                     CDSDDLVFETAVVGGAVPKNYFPAVEKGLRDCVAKGFAAGYPMVGLKATLYDGSYHPV
                     DSSEMAFKTAASLAYKNAMPKAGVAILEPIGTLTAYMPDENLGDIMGDVTKRRGRVLG
                     MGASEEKGMQELTAEVPIAEMGDFTTVLRSVTAGRGHYTLSFARYEQAPQPVADKVIA
                     DRAAENEE"
     gene            2662..4059
                     /locus_tag="ACZPE2_00165"
     CDS             2662..4059
                     /locus_tag="ACZPE2_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318292.1"
                     /translation="MSDDKFSRFREQYKTFTYEDYHITKDDKYITVSFDFKIDGLCEF
                     HPKTEIELTGLDILNDFSSPTARNIVFSLGMVELVSYWKIACPERVEIKCGFLNDRQK
                     EFWKQLYFGGLSEFFYINGIETNKDSFMTIECDEAKDNIAPICYKTAGINLIPVGGGK
                     DSAVTAELLKPFGKRNKFFTVNDQAARTETVIAAGYGEDDIIKTYRAIDKNLLELNKK
                     GFLNGHTPFSAIVAFLSLYCAYLIGGEYIVLSNESSANSGNIEGREVNHQYSKSYRFE
                     NDFTEYVGENLLSGIRYFSLLRPFSELQIAKQFALLPQYHKVFRSCNRGSKKNIWCCK
                     CAKCLFVYSILSPFLPLSTLSDVFGSEMLSDESLKDIFDGLVGFTPVKPFECVGTVSE
                     INYALMLTAQRFIKENKKMPYLLDYFYKRADKSVLDKNLLNEYNPVNNVPDDFLFAVK
                     EMYENVSECGFDVQK"
     gene            4019..5320
                     /gene="murD"
                     /locus_tag="ACZPE2_00170"
     CDS             4019..5320
                     /gene="murD"
                     /locus_tag="ACZPE2_00170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1318293.1"
                     /translation="MKMYQSAASMFKNKKVLILGFGREGMSTYWFLRNMYPDMHLTIA
                     DKNEIKLDDKNVTLICGDSYMDSLNDFDIVMKSPGIAFLDVDIKDGTLVTCQTDLFLK
                     FAPCRKVGITGSKGKTTTSTLIYDMLKEGGFDARLIGNIGVPVLEDINTVTPETIAVI
                     EMSSHQLEFTTSSPEVAVLTNIYEEHLDHYRDGFRGYVNAKLNIVRRQKEGDTFIYNA
                     TQGLDGLCDLGKIKSHKVGVKKDAPLPFAVNNEHLLGEHNRQDILFAFEAAKHFGVDT
                     AAAERAVEKFSGIEHRMEKVGTFRGITFYNDCIATIPHAVECAVEALKNVDTLIFGGM
                     DRGLDYSEFENYLKKSNIKNLIGTPATGHKICDKIEGAPQKTVYKAANLDEAVKKAYE
                     VTAKGKICLLSPAASSYNCYKNFEEKGKHYKKLVREYGKED"
     gene            complement(5466..6251)
                     /locus_tag="ACZPE2_00175"
                     /pseudo
     CDS             complement(5466..6251)
                     /locus_tag="ACZPE2_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012471.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
BASE COUNT         1618 a         1504 c         1510 g         1680 t
ORIGIN      
        1 attgcggacg cgcttcgggt gagttctcaa ccattacgca aacgggacgc ttgcctatgc
       61 tgctttcggg gagacccgta agaccggtaa gcggatttac atctgtcgcg gcggcaggct
      121 ccgctgtggt gaccggagct gacgtgtctg tttcaaaatc cttttgtttt ttaccgcagg
      181 caaaaagcat gcaggccgaa agcaaaacac aaatcgcagc cgcagaaagt ttaaacacgg
      241 tttttttgtt catttccaaa accccttgaa ctaaaagtat ttatattatt ctaccatatt
      301 tgacgatttt ttcaatacca aacggcaaaa agaggggaac aatcctcgcc gcagctcggc
      361 aaaaaccgtt ccccgattgc tgtttattct tcgttttcag ccgcgcggtc ggcaattacc
      421 ttgtcggcaa ccggctgcgg agcctgctca tagcgggcaa atgagagagt gtaatgtccc
      481 ctgcctgccg tgacggagcg aagcaccgtt gtgaaatcgc ccatctcggc tatcgggacc
      541 tcggcggtaa gctcctgcat acccttttcc tccgaagcgc ccattccgag gactctgccg
      601 cgacgttttg taacgtcgcc catgatgtcg ccgaggtttt cgtcgggcat ataggctgtg
      661 agagtgccta tcggctcaag tatggcaacg cctgccttcg gcatcgcatt tttatatgcg
      721 agcgaagcgg ctgttttaaa cgccatttcc gaggaatcaa cggggtggta agagccgtca
      781 tagagcgtgg ctttaagccc taccatcgga tagcctgccg cgaatccctt tgcaacacag
      841 tcacgaagtc ccttttcgac tgccgggaag tagttcttcg gaacagctcc gccgactacc
      901 gcggtttcga atacgagatc gtcgctgtcg cacggctcga attttatcca aacatcgccg
      961 aattggccat gaccgccgga ctgcttcttg tgcttgccct gtgcctctac cggctttctg
     1021 atagtctcgc ggtatgcaac cttcgggagc tcaagcgtta cgtctacgcc gaatttattt
     1081 ttaagctttg aaacgattac gtcaagatgc tgttcgccga gacccgagag cacctgttcc
     1141 cttgtctcct tgttaagagc aaaggtgatt gtcgggtcct cctccataag tcttgtaaga
     1201 cctgcggcaa ccttttcttc ctcacctttt ttcgacacct taacagccat cgaaaggcac
     1261 ggcttcggga aatcgactcc ggcgagcttg agtatgtttt tcgggtcaca gagcgtgtcg
     1321 cccgttttaa cgcctgccat ttttgtgaca acgcctatat cgcctgcgat gattgcgggg
     1381 gcgtcctcct gctttgcgcc ttttacaaat acgattttac ccattttctc gatttcgccc
     1441 gtgcggaaat tatatgccgc ggtgtcgggc gcgagcttgc cggaaacaac cttgaagaac
     1501 gacatcttgc ctacaaacgg gtctgctatc gtcttgaagc agacggctgc gagcggaccg
     1561 ttttcgtcgc agggtaaaat accctcctcg ccgttttcat aggtgcattt ttcgcctgcg
     1621 tagccgaatg caccgggtgc ggaccatgca aggccgtaaa gcagctgatc aacgcctgcg
     1681 caggtaagac cggataccgc gtatacgggg taaatcgagc cgtcgaaaat acctatgcaa
     1741 agacccttta atatttcctc cttggtgaat ttctcaccct cgaagaattt ttccataaga
     1801 gcgtcgtcgg tggttgcgac agcctccata aatgccgagg tcatctcttc aataacgctg
     1861 tcctctggta cgggaacctt ggtcgctttg ccgttttcgt cgtatgaaaa cgccataccg
     1921 gtcataagat taacatagca ctttgtaata ttgccctcca tatacgggat tattgtcggg
     1981 cagagcttgt tgccgtaaac ctcttttaag gcgtcaaagg tcttgtaaaa gtttctgtgg
     2041 tcggaatcgg ttttggtgac ggcaaagaat ttcgccgcac cctttttttc tgcggcacgg
     2101 aatgcttttt ccgtacccac gtcaacgcct gaacctgccg aagtgactat aagcaccgcc
     2161 tcggcggctc tgactgcctc ggctgcaccg ccctcaaagt cgaaaagacc gggagcatct
     2221 ataatattga gctttgtgtt atcccattca agtgccgcta tggcggaaga gacggacgat
     2281 ttgcggcgtt tttcctcgga atcaaagtcg agaacggtgt taccgtcggc aactctgccg
     2341 agtctgtccg aagcgccggc tatgtaaagc atagcttcgc acagcgttgt tttgcccttt
     2401 ccgccgtggc cgagcacggc aatattacgt atgttgtttg aagtgtatgc tttcacagga
     2461 aattcctcct aaaatagatg ttactgtcct tttatgtatt attcataata gttagagcca
     2521 gtatggtgaa attatagcat aattgcccaa taatagcaat tactatttta taaaattata
     2581 agaaatgtta atttcctcgt tttcttgctt tttttgcatt aaataagtat aataatatga
     2641 atgaaatttt ggaggtaaaa aatgtctgac gataagtttt cgcgctttag agagcaatat
     2701 aagaccttta cctatgagga ttatcacatt acaaaagatg ataaatatat aaccgtttcg
     2761 tttgatttca aaatagacgg gctttgcgaa tttcacccaa aaaccgaaat tgagctgaca
     2821 gggcttgata ttttgaacga tttttcgtct cccacggctc gaaacatcgt gttttcactc
     2881 ggcatggtgg agcttgtcag ctattggaaa atcgcttgtc ccgaaagagt ggaaattaag
     2941 tgcggttttt tgaacgacag gcaaaaggag ttttggaagc agctctattt cggcggcttg
     3001 tccgaatttt tctatataaa cggcatagag acaaacaagg actcgtttat gacaatagaa
     3061 tgtgacgaag cgaaggataa tattgctccg atttgctata aaaccgccgg tatcaacctt
     3121 attcccgtcg gcggcggaaa ggacagcgcc gttacggcag agcttttaaa gccgttcggt
     3181 aaaagaaata agtttttcac ggtcaacgac caggcggcgc gcaccgaaac cgtcattgcc
     3241 gcaggctacg gcgaggacga tattataaag acctatcgcg cgatagataa aaacttactg
     3301 gagcttaaca aaaagggatt tttgaacggt cacacgccgt tttcggcaat cgttgctttc
     3361 ctctcgctgt attgcgccta tctcataggc ggcgaatata ttgttctctc gaatgaatca
     3421 agcgcaaatt ccggaaacat cgagggcaga gaggtcaatc accagtattc aaagtcatat
     3481 agatttgaaa acgatttcac cgagtatgtc ggcgaaaatc tgctttctgg catacgctat
     3541 ttcagccttt tacggccgtt ttcggaattg cagattgcaa aacagtttgc gcttttgccg
     3601 cagtatcaca aggtttttcg cagctgcaac cgcggcagta agaaaaatat atggtgctgt
     3661 aagtgtgcca agtgcctttt tgtttattcg atattatcgc cgtttttgcc tctttcgact
     3721 ctctcggatg ttttcggcag cgagatgctg tccgacgaga gcttaaagga tattttcgac
     3781 ggacttgtgg gcttcacccc ggtaaagccg tttgagtgcg tcggtacggt aagcgagata
     3841 aattatgccc ttatgctcac ggctcagcgg ttcataaagg aaaataaaaa gatgccttat
     3901 ttactcgatt atttttacaa aagagcggac aagagcgtct tagataaaaa tttactaaac
     3961 gaatataacc ccgtaaacaa tgttcccgac gactttttgt ttgctgttaa ggagatgtat
     4021 gaaaatgtat cagagtgcgg cttcgatgtt caaaaataaa aaggtgctta ttctcggctt
     4081 cggcagagag ggtatgtcaa catattggtt tttaaggaat atgtacccgg atatgcacct
     4141 gacaattgcc gacaaaaacg agataaagct cgacgacaaa aacgtcacgc tcatttgcgg
     4201 cgacagctat atggacagct taaacgactt cgacatagtt atgaaatccc ccggcatagc
     4261 ctttttggat gttgatataa aagacggcac tcttgttacc tgtcaaaccg atttgttctt
     4321 aaaatttgcg ccgtgccgca aggtaggcat aacaggctcc aagggaaaaa ccacaacctc
     4381 cacgcttatt tacgatatgc ttaaggaggg cggatttgac gcacgcctca taggaaatat
     4441 cggcgttccg gtgcttgagg atattaacac ggtcacgccc gagacgatag cggttatcga
     4501 gatgtcgtcg catcagctcg aatttaccac gtcctcgccc gaggttgcgg ttctcaccaa
     4561 tatttatgag gagcatcttg accattaccg cgacgggttc cgcggctatg tcaacgcgaa
     4621 gctcaacatt gtccgtcggc aaaaagaggg agacaccttc atttataatg caacacaggg
     4681 gcttgacggc ctttgtgacc tcggcaaaat caaatcacac aaggtcggcg taaaaaagga
     4741 cgcgccgttg ccgtttgcgg tgaataacga gcatcttctc ggcgaacaca acaggcagga
     4801 tattctcttt gcctttgagg cggcaaagca tttcggtgtg gatacggcgg ctgccgaaag
     4861 agcggtagaa aaattcagcg gcatagagca ccgtatggaa aaggtcggca cgttccgcgg
     4921 aataaccttt tacaacgact gcattgcgac tataccgcac gccgtagagt gtgccgtcga
     4981 ggcacttaaa aatgttgata cgctgatttt cggcggtatg gacagaggtc ttgattacag
     5041 cgaatttgaa aattatttaa agaaaagcaa tatcaaaaac ctgataggca cacccgcgac
     5101 aggtcataag atctgcgata aaatcgaggg tgcaccccaa aaaaccgtat ataaagcggc
     5161 aaatctcgac gaggcggtaa aaaaagcgta tgaagtgacc gcaaagggca aaatatgtct
     5221 gctctcgcct gccgcttcaa gctataactg ctacaaaaac tttgaggaaa aaggcaagca
     5281 ttacaaaaag cttgtccgcg aatacggaaa agaagattaa gcaccgtaaa cttgaaaggc
     5341 ttcggcgaag tcggctgttg actctgcaag gctttaaaag ccgtaaaatg atgcgctgtt
     5401 ttgtgtgggc tcgctgcttt acggcgtgag gaataagaca aaaaggatga ttagttaaaa
     5461 aataactaca ttatcgcgcc aagcgtcagc atttcttcaa atctgttgta gagaaagtta
     5521 aaatcctcca agggggcaga ctcgtgactt tcgagagaga cttcaaatgc aggtctgcga
     5581 tgctgtgcgc cgaaccattc gcagaagctt ccgagcctct ctttttcctc tttgcggcaa
     5641 agcggatatc cgctgaccga cgagagcacc tttgccatca tggctgtctc cgagagattg
     5701 cccttctgtt cgctccaata tattttttct ccgcctacgg tgaggcaaaa cgcatatctg
     5761 aaaagctcgg cttcgcagag attcgctatc gcctttgtct cgggttcgct ctcgggcgct
     5821 ggtcctgcgt aaagctgcgg tgacggaacg gaaaaagaca ggtcgtcgta cacaatttcg
     5881 tcgtggcgaa agttaaaatt gcgcgaaata tcaacgccgc gtgcgttggc attccacttt
     5941 gagtaatctc cctttgccgc acgggcaaca agtccggcgt aaactcccgc tccgtcggct
     6001 ctttttgccg ctatatccaa ggcgtcgggc gaaacaaggg gaactatcgt tattttgcgg
     6061 ttttggagtg acttgcgtat ttttacggca cttatttttt tatcgttctt tacggcgtca
     6121 cacattcttt caaaaaatcg gagcagcagt aatgttgtga tttgctcttt tccgcgcata
     6181 ccgccgagaa aaagcacttt ttcgtttccg tcacctatgc tgagcgaaaa cacggctttt
     6241 cccgtccagc tgcgtcctac aacctcgtat caggcgaaat catattcttt tctaagcagg
     6301 gacagtatat tt
//
LOCUS       DBMDVO010000006         6203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_4921_length_6203_cov_1.276467, whole genome shotgun sequence.
ACCESSION   DBMDVO010000006 DBMDVO010000000
VERSION     DBMDVO010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_6203_cov_1.276467"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /gene="ilvD"
                     /locus_tag="ACZPE2_00180"
     CDS             <1..1651
                     /gene="ilvD"
                     /locus_tag="ACZPE2_00180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1318294.1"
                     /translation="NVTQGVERAPNRSLFYAMGYTKEELERPLIGVVSAHSEIVPGHF
                     HLDKLTEAVKAGVRMAGGTPVMIPSIGVCDGIAMGHIGMKYSLPSRELIADSVETMAM
                     AHGFDGLVLVPNCDKIVPGMLLGALRINIPTVVCSGGPMMSGLVDGVETSLSKMFEAV
                     GACKAGIIDEEKLCEFENNTCPGCGSCSGMYTANSMNCLCESLGIALPGNGTIPAVHA
                     GRTQLAKHAGMAIMELVKKDIKARDIINEKALRNALACDMALGCSSNSVLHLLAIAKE
                     AGCPMDLKVFNEMSAKVPNLCHLAPAGPTHMHDLNNAGGVRAVQAELAKKGLLDLSLM
                     TVTGKTVGENIEGAYIKNTNAIRPIDNPYSETGGIAILWGNIAKDGCVVKRSAVAPEM
                     LVHTGPARVFDSEDTAIKAIYNGEIKDGDVVVIRYEGPVGGPGMREMLNPTSALAGMK
                     LDKTVALITDGRFSGASRGASIGHVAPEAAAGGEIGLVEEGDIIDIDIPAAKVNVRVS
                     DEELAERKKHYVPHEPNVKTGWLARYASLVSSAKDGAVMKF"
     gene            complement(1768..3444)
                     /gene="ilvB"
                     /locus_tag="ACZPE2_00185"
     CDS             complement(1768..3444)
                     /gene="ilvB"
                     /locus_tag="ACZPE2_00185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR1318295.1"
                     /translation="MKLNGSDIIAEVLIEQGVDTIFGYPGGAVLNIYDALYKYRDKIH
                     HILTAHEQGASHAADGYARATGKTGVVIATSGPGATNLVTGIATAYMDSIPMVAITGN
                     VGTSYIGKDAFQEVYITGITMPITKHNFFVSDIKELAPTLREAFKIANSGRKGPVLVD
                     IPKDVTAAITEYEKAAPEVIIDNTVINEDEVKKAAALINKAKRPVIEIGGGVVSSEAS
                     DLVRMLVNKTGAPCCHTLMAAGVLGYSERLNYGLVGMHGSFVTNKAIDEADVLVAIGT
                     RFSDRVALNTNRFAHKAKVIQIDIDLSEVDKNVKVDHALVGDVKTIVMDLLHYVKQSD
                     RTDWLKTLDEYKNSDYHPVDSDKFITPHQLIRAICENVPKDTVYVTDVGQHQMWAAQY
                     LEHTDPRSFLTSGGLGTMGYGYGAAIGAKVAFPDRRVIHITGDGSFHMNMNEACTAVS
                     YNLPIITVIFDNEVLGMVRQWQTAFYGKRYSQTDPHRKTDYVKVIEGFGGKGYRAHTL
                     AEFNDVFQKALKEDKCPVWIDCAIPKDEKVLPLIPAGKTIDDIIIEDCDK"
     gene            complement(3572..>4177)
                     /locus_tag="ACZPE2_00190"
     CDS             complement(3572..>4177)
                     /locus_tag="ACZPE2_00190"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1318296.1"
                     /translation="GLYNMHYSVPPEVILSHSYFMSHPEEFYRFYRDKLLNPDVKPNI
                     AHLKLAEMEQKGKLKAVITQNIDGLHEKAGSKNVYNLHGSVYYNHCMKCGKAYAFTDM
                     LSCKGVPHCTCGGIIKPDVVLYEEPLNESTVNGAVRHIMNADMLIVAGTSLTVYPAAG
                     LIRYFRGRNSVLINRDANVGDDEFSIVIHGKVGEVLGSLVV"
     assembly_gap    4178..4187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4188..4286)
                     /locus_tag="ACZPE2_00195"
     CDS             complement(<4188..4286)
                     /locus_tag="ACZPE2_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1318297.1"
                     /translation="MTNTEKLQKMYDESDYVVFFGGAGVSTESGIPD"
     regulatory      4483..4642
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            4781..6136
                     /gene="mgtE"
                     /locus_tag="ACZPE2_00200"
     CDS             4781..6136
                     /gene="mgtE"
                     /locus_tag="ACZPE2_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1318298.1"
                     /translation="MFYSHKSFALCFLELLKEKNYSDLKKLLSDMNEADIASILTDVP
                     EETLPLLYRILPKELAADVFVNMDSDAQEILIRAFSDTELKEVLDELYVDDAVDIIEE
                     MPATVVKRILQHTDPNIRKSINEILKYPEDSAGSIMTIEYVDLKTYMTVDDAFTRIRR
                     TGVDKETIYNCYVTDENRKLLGVVTAKKLLLSEKSEKIADIMETNIISTNTLADQEIV
                     AEKFRKYDLLAMPVVDQENRLVGIITIDDAMDVLQDENTEDIEKMAAITPTDKPYLKM
                     NVFEIWKKRFPWLLLLMVSATFTGKIITHFEDSLQSCVILTAFIPMLMDTGGNGGSQA
                     SVTIIRGLSLGDIEFKDIFKVIWKEFRVSILCGLTLAAANFIKMMIIDRVTVLVALTV
                     CLTLIFTIMLAKFVGCTLPILAKKIGFDPAVMASPFITTIVDALSLLIYFNVAEAVLK
                     L"
BASE COUNT         1689 a         1492 c         1399 g         1613 t
ORIGIN      
        1 aaatgtaaca cagggtgtag agcgtgctcc taacagaagc cttttctacg ccatgggata
       61 tacaaaggaa gagcttgaaa gaccgctcat cggcgttgtt tcggcacaca gcgaaatagt
      121 tccgggtcat tttcaccttg acaagcttac agaagcagtt aaagccggcg tgagaatggc
      181 aggcggcaca cccgttatga ttccgtcaat cggcgtatgc gacggtatag cgatgggtca
      241 tatcggtatg aaatactctc tgccgtcacg cgagcttatt gccgacagcg ttgaaaccat
      301 ggctatggct cacggcttcg acggtcttgt cttggttccg aactgcgaca agatagtccc
      361 cggaatgctt ctcggcgcac tcaggataaa tatccccacg gtagtctgct ccggcggacc
      421 tatgatgtcg ggtcttgttg acggcgttga aacctcgctc tctaagatgt tcgaggctgt
      481 cggcgcgtgc aaagcaggca ttatagacga agaaaagctc tgtgaatttg aaaataacac
      541 atgccccggc tgcggctcat gctccggaat gtataccgca aacagtatga actgcctttg
      601 cgaatcactc ggaatcgcgc ttcccggaaa cggcaccata cccgccgttc acgctggccg
      661 cacccagctt gcaaagcacg ccggaatggc gattatggag cttgtgaaaa aagacattaa
      721 agcccgcgat attataaatg aaaaggcgct ccgcaacgcg ctcgcctgcg atatggctct
      781 cggctgctcc tcaaacagcg ttttgcatct gcttgcaatc gcaaaagagg caggctgtcc
      841 catggacctc aaggtattta acgaaatgag cgcaaaggtc ccgaatttat gtcacttggc
      901 ccccgcaggc cccacacata tgcacgacct caacaacgca ggcggcgtaa gagcggttca
      961 ggcggagctt gctaagaaag gactgcttga cctctcgctt atgaccgtta ccggaaaaac
     1021 cgtaggtgaa aacatagagg gagcatacat aaagaacaca aacgcgatac gtccgataga
     1081 caatccatat tcggaaaccg gcggcatagc gattctttgg ggcaatatag caaaggacgg
     1141 ctgcgtcgta aaacgcagtg cggtagcacc cgaaatgctc gttcacacag gcccggcgcg
     1201 tgtatttgac agcgaggata cagcaattaa agcaatttac aacggcgaaa tcaaggacgg
     1261 agatgttgtt gtaatccgtt acgaaggacc cgtaggcggc cccggtatgc gagagatgct
     1321 caaccccacc tcggcacttg ccggtatgaa gcttgacaaa acggttgccc ttatcacgga
     1381 cggaagattc agcggagctt caaggggtgc ttcgataggc cacgttgctc ccgaagcagc
     1441 cgcaggcggt gaaatcggcc ttgtagaaga gggcgacatc attgacattg acattcctgc
     1501 cgcaaaggta aatgtcagag tatccgacga ggagcttgca gagcgtaaaa agcactatgt
     1561 tcctcatgag cctaacgtca aaacaggctg gctcgcaaga tacgcgtcgc ttgtttcctc
     1621 cgccaaggac ggcgccgtta tgaaattcta aatgaacaaa acaaagctcc gccaaagata
     1681 attctctgtg cggagctttt attgtataaa caaaaagagt caatagtcaa aagactactg
     1741 actcttttat ttatacggac actttaacta tttgtcgcaa tcctcaataa ttatatcatc
     1801 tatcgttttg cctgccggta taagaggaag caccttttcg tctttgggta tagcgcagtc
     1861 aatccaaacg gggcacttat cctccttaag tgccttttgg aatacgtcgt taaactcggc
     1921 taaagtatgc gctctgtagc cctttccgcc gaagccctct attaccttaa cataatcggt
     1981 tttgcggtgc ggatcggtct gcgaatagcg tttgccgtag aatgcggtct gccactgacg
     2041 aaccataccg agaacctcgt tgtcaaaaat cacggttatt atcggcaaat tatagctgac
     2101 tgcggtgcac gcctcattca tattcatatg gaaagagccg tcgcccgtga tatgtataac
     2161 tctcctatcg gggaacgcaa ccttggcgcc tattgccgct ccgtagccgt agcccatagt
     2221 gccgagaccg ccgcttgtca aaaacgaacg cggatcggta tgctccaaat actgagccgc
     2281 ccacatctgg tgctggccga catcggtaac ataaacagta tccttcggta cattttcgca
     2341 aatcgccctt ataagctgat gcggcgtaat aaatttgtcg gaatctaccg gatgataatc
     2401 gctgtttttg tattcgtcaa gggttttaag ccaatcggtc cggtcgcttt gctttacgta
     2461 atgtaaaagg tccataacga tagtcttaac atcgcccact aaagcgtgat cgactttaac
     2521 atttttatcg acctcactca agtcaatatc aatctgtatt acctttgctt tatgagcaaa
     2581 acgatttgta ttgagagcga ctctgtcgga aaatctcgtg cctatcgcaa caagcacgtc
     2641 tgcttcgtca atagctttat tggtaacaaa gctgccgtgc ataccgacaa ggccgtaatt
     2701 cagcctttcg ctgtagccga gaactcccgc cgccataagc gtatgacagc acggcgcgcc
     2761 cgttttgttt acgagcatac gcacaaggtc cgaggcttcc gacgacacca cgccgccgcc
     2821 tatttctata accggtcttt ttgccttgtt tataagggcc gcagcctttt tgacctcgtc
     2881 ctcatttatt actgtattgt caattataac ctcgggagca gctttttcgt attcggtaat
     2941 cgccgccgta acgtccttgg gtatatcaac aagtacggga cctttccttc cggaatttgc
     3001 tattttaaaa gcctctctga gcgtcggagc aagttcctta atgtcgctga caaaaaaatt
     3061 atgctttgta ataggcattg taatgcctgt aatgtatact tcttggaaag cgtccttacc
     3121 tatataagat gttccgacgt tgcctgttat ggctaccata ggtatgctgt ccatatatgc
     3181 cgtggcaatt cccgtaacaa ggtttgtggc gccgggaccg cttgtcgcaa ttacgacccc
     3241 cgttttgccg gttgcccttg catagccgtc agcagcatgg gacgcgccct gctcgtgagc
     3301 tgtcaggata tgatgaattt tatctctgta tttataaagt gcatcataga tattgagtac
     3361 cgctccgccg ggatagccga aaattgtgtc taccccctgc tctataagca cctcagcgat
     3421 tatatccgat ccgttaagct tcatcttttt ctcctgccga taaatagatt taaaatatat
     3481 tttatatccg ttgtcgataa atgtcaataa aaagactgta tatatcgccg tttcgcgcac
     3541 aaaacagtct tacagccgct ttgatttaca gctatacgac aaggctgccg aggacttcgc
     3601 ctaccttacc gtgaataaca atggaaaact cgtcatctcc tacatttgca tcgcggttta
     3661 tgagtaccga atttctgccg cgaaaatatc ttataagccc tgccgcagga taaacggtaa
     3721 gcgacgtacc ggcaacaatc agcatatcgg cgttcataat atgccttacg gcaccgttaa
     3781 ccgtgctttc gttgagcggc tcctcataaa gcactacatc gggctttatg atgcctccgc
     3841 aggtgcaatg gggaacacct ttacacgaca gcatatctgt aaaggcgtaa gccttaccgc
     3901 atttcataca atgattataa taaacgctgc catgcaggtt ataaacgttt ttgcttccgg
     3961 ctttttcgtg aagtccgtct atattttgcg ttattaccgc ttttaattta cctttttgct
     4021 ccatctcggc aagcttcaga tgcgcgatat tcggttttac atcgggattt aacagcttat
     4081 ctctgtaaaa cctgtaaaac tcctcgggat gagacataaa atacgagtga ctgaggataa
     4141 cctccggcgg cacgctgtaa tgcatattgt aaagcccnnn nnnnnnnatc aggtattccg
     4201 ctctcggtag agacgcccgc tccgccgaaa aacaccacgt agtcgctctc gtcatacatt
     4261 ttttgaagtt tttccgtatt cgtcattttt ccgctccttt gccgtatttc ctttttctat
     4321 tgtattatat taacttttca agtaattttc aatgaatttt gccttgtaaa acgcactata
     4381 tttcaccgtt ttttaaaaca ttgattttat attcaaaaaa gtgcttaaaa gggttgacaa
     4441 acagccgaaa tgagccgata ataataaggc ttaaagcaaa taaataaata tcggtaggta
     4501 aggctcccac agggatacgg gctgctgccg cgaaaagtgg agacactgta agcaggtcaa
     4561 caggccacat cgaaaagtaa ggtgtgttct aacgcagttt catcgccctc tgaagctaaa
     4621 gcttgaacga aatattttta tcaattccgc tcatatcgcc tatcgatatg agcgttttat
     4681 attgtgaggt gaaaaataat ggacgcagga agcgcaaaaa gtgcgaagcc caagccgcga
     4741 tctagcggct gcgtgaatag cataacgcct ttggcgttca ttgttttatt cacataagag
     4801 ctttgcgctt tgtttccttg aattattaaa agaaaaaaat tattccgatc tcaaaaagct
     4861 cctctccgat atgaacgagg cggatatcgc atcaatactc accgacgtac cggaggaaac
     4921 gctgccgctt ttataccgta tattaccgaa ggagcttgcg gcagacgtgt ttgtaaatat
     4981 ggattcggac gcgcaggaga tactcataag agccttcagc gataccgagc ttaaagaggt
     5041 tctcgacgag ctgtatgtag acgatgccgt tgacatcatt gaagaaatgc ctgcaacggt
     5101 ggtcaagaga atactgcagc acaccgatcc gaatatccgt aaaagcataa atgaaatact
     5161 caaatatccg gaggactctg ccggcagcat aatgactatt gagtatgttg acctcaaaac
     5221 atacatgacg gttgacgacg cttttacgag aatcaggcgc acaggcgtag acaaggaaac
     5281 catttataac tgctatgtaa ccgatgaaaa ccgcaaactt ctcggcgttg taaccgcaaa
     5341 gaagctgctg ctttccgaaa agagtgaaaa aatagcggat ataatggaaa caaacattat
     5401 atccacaaac acattagcgg accaggaaat agttgccgaa aagttccgta aatatgacct
     5461 tttggcaatg ccggttgtcg atcaggaaaa ccgtctcgta ggtataatca ccatcgacga
     5521 cgccatggac gttcttcagg acgagaacac agaggatatt gaaaaaatgg cggctataac
     5581 ccccacggac aagccgtatc ttaaaatgaa cgtctttgag atttggaaaa agcgtttccc
     5641 gtggctcttg cttttgatgg tatccgcaac cttcaccgga aaaataataa cgcatttcga
     5701 ggactcgctt cagtcctgcg tcatactcac ggcgtttatc ccgatgctta tggatacagg
     5761 cggaaacggc ggaagtcagg cgtcggtaac gataatccgc ggactctcgc tcggcgacat
     5821 tgaattcaaa gatattttca aggttatctg gaaggaattt cgcgtatcga tactctgcgg
     5881 tcttacactt gccgctgcca atttcataaa gatgatgatt atagacagag taacggtgct
     5941 tgtagccctc acggtttgct taacgcttat atttacaata atgcttgcta aattcgtagg
     6001 ctgcacacta cctatactcg ctaaaaaaat aggctttgac ccggcggtaa tggcaagtcc
     6061 gtttataacc acaatagttg atgccctgtc gcttctcatc tacttcaacg tggcggaagc
     6121 ggtgctgaag ctataaaaaa ggaacaaaca aaaaaaggct gcaatgcagc cttttttatt
     6181 tgcttgattt tagattcagt gct
//
LOCUS       DBMDVO010000007         6057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_5104_length_6057_cov_1.300537, whole genome shotgun sequence.
ACCESSION   DBMDVO010000007 DBMDVO010000000
VERSION     DBMDVO010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_6057_cov_1.300537"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="ACZPE2_00205"
     CDS             complement(<1..972)
                     /locus_tag="ACZPE2_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1318299.1"
                     /translation="MDKLLTVVIPSYNVEKFLNQTLDSFVCDEAVMKKFEVIVVDDGS
                     KDNTAKIGKEYADKYPDTFRVISKENGGHGSTINCGIRNAVGKYFKVVDGDDWVNTAD
                     FVKLVEDLEKTESEYVFTNYYEYYDDVNRQKAVDFPQFKDGTEYDFADVANKVYIPMH
                     ALVIKTSVLKDNNIVIDEKCFYVDVEYVMFPVPFVNKVTFFDLHVYMYRLALSTQSVS
                     ILGFQKHINDHLRVTFHMFDFYKDYISSDKADSAKADYMRTCISDLIITQSAIYSSYP
                     DSDMENRNRFMEFDRKAKELSPEIYELSSVKSGKLRLLRKYNFKHYRL"
     gene            complement(1041..2471)
                     /locus_tag="ACZPE2_00210"
     CDS             complement(1041..2471)
                     /locus_tag="ACZPE2_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1318300.1"
                     /translation="MKESILRKGSQATGFKLLFLALTLLSFNSLTANRSALTWIAYAV
                     TAIGIAVLVIRVVCFKKYLHAKGLFLLIAFCGSYALSALLTRQYGLSENAKALVWMAF
                     QYFILYTYDTTSDGTAEKKEFYIISHFFMAYTFLAVAAGTVMLFADYYSYTEVNGVVV
                     ILGFLWNRLWGVYSDPNYGAVFSAITIIMSLYFYKDAKKPLKALYIINIVLQICYIAF
                     SDSRTGMVSLFCALLVYVYLTALRSKKLEAKKAFARGVICVLLAVTAAVASFAAIKVV
                     SVSTSEFKKWQYEHIISSDKDKTDAQKEKDKQKLEIGRQSNDINGDVSNRRFAIWGSG
                     LEIFKTKPLTGVTFRNYVPYAEDKLPDTYLVNNDFIEFHSMHNSFVDILVSQGILGVV
                     IIAAYIILVLVLIFKNFFKFKGEKYKYNTALLSIIAPIFASMMFYSETFYMNTGGAFL
                     FWLALGYLIQSVTSKNPETKEITPGK"
     gene            complement(2481..3572)
                     /locus_tag="ACZPE2_00215"
     CDS             complement(2481..3572)
                     /locus_tag="ACZPE2_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1318301.1"
                     /translation="MLFGYIMNGKAGGIDKYMLNFFEHFSPGEVHFDFLTTQIDNELK
                     EKLESKGAGLFEIPTLKNPRAQYKAICDIIKKNGYDTAYFNISTAICYPGPKAAHDCG
                     VKKVIIHSHSAWYDCSNPKKRALMIKLNNLCRNFLYKYGTDFYACSALAGKWIFPEKI
                     VGSDKFKLVKNGIDLASFTYSPEKREAMREKLQLQDKFVIGSVGNFLYPKNHDFMVRV
                     FSELCKIDSDARLLLVGDGLLFDNVKEQVKSLGLADKVIFSGRQTDAYNYMMAMDIYL
                     MPSHFEGFGIAAVEAEATGLMCCLSDHIPSDAVITENCVTLSIDKDGDAKVWAEKIEK
                     AKGYKRIDRTDEVRRAGYDLSAQNFYELV"
     gene            complement(3598..4665)
                     /locus_tag="ACZPE2_00220"
     CDS             complement(3598..4665)
                     /locus_tag="ACZPE2_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1318302.1"
                     /translation="MSAPYFSVIMPVYGVEKYLRDTLDSVLRQTFADFELILVDDCSK
                     DGSGKICDEVAASDGRVRVIHKEKNGGASSARNLGTKSASGKYLYYMDSDDKIDSDIF
                     EKMYSASRDASPTVVMFGAVEEYYDKTGKLYMTNPVSYESKALSGKENVAKEVIKIEN
                     TTLFGYLWNKIYLKDAVFSSGVTFCDMPLNEDFKFNIDFFRYVDSMVILDDTAYHYAK
                     RDNQSLTCRFVKNYFELQEMRINELLGFYRENGMCDGDILKNLAGIYVRSVFSALEKN
                     NDKRSGMSGKDRKDWLKAQLESELYKELMPYAEPDSRLVKIMANWLKGGNTGVLLFTG
                     KFISAVKKSFPSLFSGLKLKK"
     gene            complement(4691..5749)
                     /locus_tag="ACZPE2_00225"
     CDS             complement(4691..5749)
                     /locus_tag="ACZPE2_00225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005967430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1318303.1"
                     /translation="MNDVKVSVIMPVYNVENYVGRAIESIQNQTLTDWEFFAVDDGTP
                     DNSGKICDEYAAKDPRIKVIHKENGGAPSARNVAIDKAVGKYMYFMDSDDWAEKTMLQ
                     DMVDLAEKNDLQLVVSGYYIDTYYSDTEKFTQEQVYKDKIYKTQAEFRADAHNLFDRN
                     LLYTPWNKLFSGDYIRDNKLYFPQTFWDDFPFNLSVVRDVERVGVTSKKYYHFIRQRA
                     ESETAKYRSDMYEKREEENEWMRELYEHWGIMTPEIEEFLSRRYIERIVGCVENVTNK
                     NCTLPAGEKKAQIRKMINDPKARAAVSAAVPKSKYMKLMLIPIKMKSTALTYLEGKVI
                     SSVKSGNTKLFAKLKAGR"
     gene            complement(5770..>6057)
                     /locus_tag="ACZPE2_00230"
     CDS             complement(5770..>6057)
                     /locus_tag="ACZPE2_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1318304.1"
                     /translation="TDEKTKFTRIIEHKHFEFGTQEKTYITREYPSEWQEGMEPYYPV
                     NDEKNQSLYSKYSDLSNGESNIIFGGRLAEYKYYDMDKVIASALAVTDKLF"
BASE COUNT         1573 a         1450 c         1238 g         1796 t
ORIGIN      
        1 gagcctgtaa tgcttgaaat tatatttgcg aagcaaacgc aattttccgc tcttaacgga
       61 gctgagctca tatatttcgg ggcttaattc ctttgccttg cggtcgaatt ccataaagcg
      121 gtttctgttt tccatatcgc tgtcgggata gctcgaatat attgcgctct gcgtaattat
      181 aaggtctgat atgcaggtcc tcatataatc cgcttttgcg ctgtctgcct tatccgagga
      241 aatatagtcc ttgtaaaagt caaacatatg gaacgtaacc ctgagatggt cgtttatatg
      301 cttttggaac ccgagaatac tcacgctctg agttgagagc gcaagcctgt acatataaac
      361 atgcaggtca aagaatgtca ccttgtttac aaagggcacc gggaacataa catattcgac
      421 atccacataa aagcactttt cgtcaataac aatgttatta tctttaagca cgctcgtctt
      481 tattacaagg gcgtgcatag gtatataaac cttatttgca acatcggcga agtcatattc
      541 cgtaccgtct ttgaactgcg gaaaatcaac agccttttgg cggttaacat cgtcataata
      601 ctcataatag tttgtaaaca catattcgga ttcggttttt tcgagatcct cgacgagctt
      661 cacaaaatca gcggtgttga cccagtcgtc accgtccaca accttaaagt atttgccgac
      721 ggcattgcga atgccgcagt taatggtgga gccgtgaccg ccgttttcct ttgagataac
      781 tctgaatgta tcggggtact tatcggcgta ctccttgcct atttttgcgg tgttatcctt
      841 tgagccgtcg tcaaccacga taacctcgaa ttttttcatt acagcctcgt cgcaaacaaa
      901 cgagtcaagc gtctgattca aaaatttttc gacattgtac gacggtatta caaccgtaag
      961 aagcttatcc atatttttca cctcaataat aagttccgtg aagcagtatt attctctccg
     1021 aaacaatacg atttaaaaat ttatttgccc ggcgttattt cctttgtctc gggattcttt
     1081 gatgtgacgg actgaatgag atagccgagc gcaagccaga agaggaacgc tccgcctgtg
     1141 ttcatataaa acgtctccga atagaacatc atagaggcaa atatcggagc tataatgctg
     1201 agcagagcag tattatattt gtatttttcg cccttaaatt taaagaaatt cttaaatatg
     1261 agcaccagca ccaatattat atatgctgcg ataatcacaa cgcccaaaat gccctgactt
     1321 acaagtatgt cgacaaaact gttgtgcata ctgtggaatt caataaaatc attgttgaca
     1381 agataagtgt cggggagctt atcctcggca taaggaacat agttcctgaa ggttacgccg
     1441 gttaacggct tcgttttgaa tatttcgagt ccgctgcccc aaatagcaaa gcgacggttg
     1501 ctcacgtcac cgtttatgtc gttactctga cggccgattt caagcttctg cttgtccttt
     1561 tccttctgag catcggtttt atccttatcg ctgcttatga tgtgttcata ctgccatttc
     1621 ttgaattcgg aggttgaaac cgacacaacc tttatcgcgg caaaggatgc gactgccgct
     1681 gtaacagcaa gcaaaacgca tattacgccc cttgcgaacg ctttttttgc ttcgagcttt
     1741 ttgctcctga gcgcggtgag atacacatag accaaaagcg cgcagaacag cgataccata
     1801 ccggttcgcg aatccgaaaa agctatatag catatctgta aaacgatatt tatgatgtat
     1861 aaagccttta acggcttctt tgcgtccttg taaaaataca ggctcattat tatggttatt
     1921 gccgagaata ctgcaccgta attggggtcg gaatacacac cccaaagcct gttccaaagg
     1981 aatccgagaa ttacaactac tccgtttacc tccgtatacg aatagtaatc ggcaaagagc
     2041 ataaccgttc ccgccgcaac agccaggaat gtatacgcca taaagaagtg tgaaattatg
     2101 tagaattcct ttttctccgc ggtaccgtcg gacgtggtgt cataagtgta cagtataaaa
     2161 tactggaacg ccatccaaac gagtgccttt gcgttttccg acagcccgta ctgccttgta
     2221 agcaacgccg acagcgcata gcttccgcaa aatgcaataa gcaagaataa gccttttgca
     2281 tgtaagtatt ttttgaagca gacgactctt attacaagaa ccgctattcc tatcgccgtt
     2341 accgcatacg caatccaagt aagtgccgag cggtttgccg taaggctgtt aaacgataac
     2401 agcgtaagcg caaggaaaag cagcttaaag ccggtcgcct gcgacccctt cctgagtata
     2461 ctttctttca tattaactcc tcacaccaat tcataaaaat tctgcgctga aaggtcatat
     2521 ccggctctcc gtacttcatc ggtacggtca atccgtttat agcccttggc tttttctatt
     2581 ttttccgccc aaacctttgc gtcaccgtct ttgtcaatcg agagggtaac acagttttcc
     2641 gttatcacgg cgtcggacgg tatatgatcg ctcaggcagc acataagccc ggttgcttcg
     2701 gcctccacag cggctatacc gaagccctcg aaatgcgacg gcataagata tatatccata
     2761 gccatcatat aattataagc atcggtctgc ctgcctgaga atataacctt gtccgctaag
     2821 ccgaggctct ttacctgctc ctttacattg tcaaaaagca agccgtcacc gacgagtaaa
     2881 aggcgtgcgt cgctgtcgat tttgcacagc tcggaaaata cacgcaccat aaaatcgtga
     2941 tttttgggat aaaggaaatt tccgacactg cctatgacga atttatcctg caattgcagt
     3001 ttttcgcgca ttgcttccct cttttcggga gaatacgtaa agcttgcaag gtcaataccg
     3061 ttttttacaa gcttaaactt gtccgacccg acgatttttt cggggaatat ccatttaccc
     3121 gcaagagccg agcaggcgta aaagtcggtt ccgtatttat aaaggaaatt gcggcaaagg
     3181 ttattgagct ttatcataag cgcgcgcttt ttcgggtttg aacagtcata ccacgcactg
     3241 tgagaatgta tgataacctt ttttactccg cagtcgtgcg ccgctttggg tcccggatag
     3301 catatcgcgg tagaaatatt gaaatatgcg gtatcatatc cgtttttctt tattatatcg
     3361 caaatcgcct tatactgagc acgcggattt ttaagagtcg gtatttcaaa aagccccgcg
     3421 cccttgctct caagcttttc cttaagctca ttatctattt gtgttgtgag aaaatcaaaa
     3481 tgcacctcac caggggaaaa gtgctcaaaa aagttgagca tatacttgtc aattccgccg
     3541 gcttttccgt tcataatata gccgaaaagc acctttttca actgtttgcc ctccacatta
     3601 tttttttagc ttaagccccg aaaacagcga cgggaagctt tttttgaccg ccgaaataaa
     3661 tttgcccgtg aaaagaagca cgcctgtatt tccgccttta agccagttcg ccataatttt
     3721 tacaagcctg ctgtcaggct cggcgtaagg cataagctcc ttgtaaagct cactttcaag
     3781 ctgagccttg agccagtctt ttctgtcttt cccggacatt ccggagcgtt tgtcattatt
     3841 tttttcgaga gccgaaaata cgcttctgac atatatgccg gcaagatttt taagtatatc
     3901 tccgtcacac ataccgtttt cacggtaaaa accgagcagc tcgtttatac gcatttcctg
     3961 aagctcaaag taatttttga caaagcggca cgtaaggctt tgattgtctc tcttggcata
     4021 gtggtatgcc gtgtcgtcaa gaattaccat ggaatcaaca tatctgaaga aatctatatt
     4081 aaatttaaaa tcctcgttaa gcggcatatc gcaaaatgta actccggagg aaaacaccgc
     4141 gtctttaaga taaatcttat tccaaaggta gccgaaaagc gtggtgtttt cgattttaat
     4201 tacttctttc gccacattct ctttacccga gagagctttg ctctcatatg acacgggatt
     4261 tgtcatatac agcttaccgg tcttgtcgta atactcctcc accgcgccga acatcacaac
     4321 cgtgggagaa gcgtcacgcg aagccgaata cattttttca aatatatcgc tgtctatttt
     4381 atcgtcggaa tccatataat agagatattt gcccgaagcg gactttgtgc cgagatttct
     4441 cgcgctgctc gctccgccgt ttttctcctt atggataacc cgaaccctgc cgtccgaggc
     4501 tgcaacctca tcgcatatct tgccgctgcc gtccttggaa cagtcgtcga cgagtataag
     4561 ctcaaagtcg gcaaaggttt gccgaagaac gctgtcaagc gtatccctga gatatttttc
     4621 gacgccgtaa accggcatta taacactgaa atacggtgcg ctcatatttt tcacagcctt
     4681 tcggattatt ttatcttccc gctttaagct ttgcgaaaag cttcgtattg cccgacttaa
     4741 cggaagaaat gaccttgccc tcaagatatg tgagcgcggt gctcttcatc ttaatcggta
     4801 taagcataag cttcatatac tttgacttcg gcacagccgc agaaaccgcc gcgcgcgcct
     4861 tggggtcgtt tatcatcttt cttatctgcg cctttttttc gccggcaggc aaggtgcagt
     4921 ttttgtttgt aacgttttca acgcatccga ctatgcgctc gatatatctt ctcgagagaa
     4981 attcctctat ctccggagtc ataattcccc agtgctcata gagctctctc atccactcgt
     5041 tctcctcctc gcgcttttcg tacatatcgc ttctgtactt ggcggtttcg gactcggctc
     5101 tctgacggat aaagtggtaa tacttctttg acgtcacgcc gactctctca acgtcacgga
     5161 caacggagag gttaaagggg aaatcatccc agaaggtctg cgggaaatag agcttgttgt
     5221 ctcttatata atcgcccgag aagagcttat tccacggagt gtagagaaga ttgcggtcaa
     5281 aaaggttgtg tgcgtcggct ctgaattcag cctgagtttt gtatatctta tccttgtaga
     5341 cctgctcctg cgtgaatttt tcggtgtcac tgtaatatgt gtcgatatag taacccgaaa
     5401 caacgagttg taaatcgttt ttctcggcca aatcaaccat atcctgaagc atggtttttt
     5461 cagcccagtc gtcggagtcc ataaaataca tatattttcc gacagcctta tctatagcga
     5521 cgtttctcgc cgagggagcg ccgccgtttt ccttatggat aacctttata cgcgggtcct
     5581 ttgcggcgta ttcatcgcag attttaccgc tgttatcggg agttccgtcg tctacggcaa
     5641 aaaattccca atcggtaagg gtctgatttt gtatactctc gatagccctg ccaacgtaat
     5701 tttcgacgtt gtaaacaggc attataacac taacttttac atcattcata gcctttgacc
     5761 gtccctcgct caaaataatt tatctgttac cgccaaagcc gaagctatga ccttatccat
     5821 atcataatat ttatactcgg caagtctgcc gccgaagata atattgcttt cgccatttga
     5881 aaggtcgctg tatttgctgt aaagcgactg attcttttcg tcgtttacgg ggtaatacgg
     5941 ctccataccc tcctgccact cggacggata ttctcttgtt atatacgtct tttcctgagt
     6001 accgaattca aagtgcttgt gctctataat tcttgtgaat ttggtctttt cgtcagt
//
LOCUS       DBMDVO010000008         5467 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6012_length_5467_cov_1.277488, whole genome shotgun sequence.
ACCESSION   DBMDVO010000008 DBMDVO010000000
VERSION     DBMDVO010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_5467_cov_1.277488"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="ACZPE2_00235"
     CDS             complement(<1..174)
                     /locus_tag="ACZPE2_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1318305.1"
                     /translation="MYKNIFDTHAHYEDEQFDCDRDELLSSFPENGISGVVTCGTDII
                     TSEKSIALAEKYPF"
     gene            complement(174..2120)
                     /gene="metG"
                     /locus_tag="ACZPE2_00240"
     CDS             complement(174..2120)
                     /gene="metG"
                     /locus_tag="ACZPE2_00240"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1318306.1"
                     /translation="MSEKPKFYITTAIAYTSRKPHIGNSYEIVLTDAIARYKRLQGYD
                     VFFLTGTDEHGQKIEEYAKEAGVSPKEYVDKVSGEIRAICDVLNTTYDKFIRTTDPYH
                     EKIVAKIFKKLYEQGDIYKSEYEGMYCTPCESFWTESQLVDGKCPDCGREVKKAKEEA
                     YFLRLSKYQKQLEDYIESHDDFIYPEARKKEMLNNFIKPGLQDLCVSRTSFKWGIPVT
                     FDPNHVIYVWIDALSNYITALGYDPDGSSDMYKKYWPADVHIIGKDIVRFHTIYWPIM
                     LMALGEPLPKQVYGHPWLLFGEDKMSKSRGNVIYADDLVKDFGVDAVRYYLLSEMPHA
                     QDGSITYESLIERYNSDLANTIGNLVNRTVAMQNKYFDGVIDAPTDKEPLDDEISSLA
                     LKTVAEVDRCFDEYKISDATENILTLARRLNKYIDETTPWALAKDESKKGRLGTVLYN
                     LLEGIRFLAVLLTPFMPDTAEKIFEQIGTNERSYESLKAFGALKSGEKVGTPTPLFNR
                     IDGEKLIEELTAKQKEQQKAEKKMINSLEGVAEIGIDDFAKVELRVAEVKACEKVPKA
                     KKLLKLTLDDGTGKDRTVASGIAKWYAPEDLVGKKVVVVANLKPAVLCGVESNGMILA
                     ADCSEDDVKVVFVDKSMPNGSKIR"
     gene            complement(2146..3000)
                     /gene="folD"
                     /locus_tag="ACZPE2_00245"
     CDS             complement(2146..3000)
                     /gene="folD"
                     /locus_tag="ACZPE2_00245"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR1318307.1"
                     /translation="MATIIDGKAVSAFLKDGIKKECLELKEKGVSVCLAVIIVGDDPA
                     SRTYVNNKKKACEAVGIISREYALPADTSMAELLALIDELNNDREVNGILCQLPLPKG
                     LSDELVIKAISPKKDVDAFSAENVGHIMIGDHTFLPCTPAGIMEMLKYYNIDVCGKEC
                     VVVGRSNIVGKPMAMLLLQQNGTVTICHSKTKNLKEVTRRADILVAAVGKPYFVTADM
                     VKDGAVVIDVGMDRNSEGKLCGDVDFAEVSKKASYITPVPGGVGPMTIATLLKNTVTA
                     AKQQNGLI"
     gene            complement(2984..>3276)
                     /locus_tag="ACZPE2_00250"
     CDS             complement(2984..>3276)
                     /locus_tag="ACZPE2_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318308.1"
                     /translation="VEGLRTDSLYIGSSNLRVSQLLSMVIAVVCLAILTAKLVSLKKH
                     PKPFSPVEVREDAEKSDALKDGNEDKKKNKISAEKRREMMRQKERKNDGDDN"
     assembly_gap    3277..3286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3287..4008)
                     /gene="lgt"
                     /locus_tag="ACZPE2_00255"
     CDS             complement(<3287..4008)
                     /gene="lgt"
                     /locus_tag="ACZPE2_00255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1318309.1"
                     /translation="MTFLTDGTAVKFTGIDHTFNVYSTFVEFNIGSSTFSIKFYGLII
                     AFGFLLAVLFGGRMAYKWKMDLNKMLDVLLYGTIGGIIGARLYYVAFEWDYYSQNLSE
                     IPKIWNGGLAIYGGLIGGIIAAAITCKVDKMDFLKLLDLAGMSFLIGQGIGRWGNYAN
                     QEAFGTNTTMPWGMTSSKVVDYIVSNQALFRAKGIEMSPNVPVHPTFLYESVWCLLGF
                     LVIYIICRKFYKFSGQLILCYGV"
     gene            complement(4024..5301)
                     /gene="yfmH"
                     /locus_tag="ACZPE2_00260"
     CDS             complement(4024..5301)
                     /gene="yfmH"
                     /locus_tag="ACZPE2_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIR1318310.1"
                     /translation="MNNVTEIKNEKLGESYYEIKHQSGLKILVYPKKNYASSYAMFGT
                     RYGSIDTQFKLSGEKEFTEVPEGIAHFLEHKLFESEDLDAFQRYAATGASANAYTSFD
                     KTCYLFSCSGDFKGSLEILLDFVTHPYFTPETVEKEQGIIGQEIKMCQDEPSWEVLFG
                     MLRGMYKKHPVRIDIAGTVESISHITAELLYKCYGTFYNLSNMVLCVAGNVDVDTVLS
                     VADKVLKKQPEKKIERKFHEEPKEVCESYVEEFLPVATPVFALGYKEDVKTPERSLKD
                     VICSNIILELVAGKTSKLYSDMLSDGLINTAFGFEFFEGYGYACQIYSGESRDPEKVR
                     ELISGKINELKRDGVDEKDFERIRRKLYGRAVFDFNDIESIANEMVSATFNGETVFSG
                     MEVLENLTKEDVEKRLLSSVDTENCVLSVIKSN"
BASE COUNT         1301 a         1428 c         1176 g         1552 t
ORIGIN      
        1 aaacggatat ttttccgcaa gagctatcga cttctcgctc gttattatgt ccgtaccaca
       61 ggtgacgacg cccgatatac cgttttcggg aaacgaagac agcagctcat cgcggtcgca
      121 gtcaaactgc tcgtcctcat agtgcgcgtg cgtatcaaat atatttttat acattatctt
      181 atttttgagc cgttcggcat actcttgtca acgaacacaa ctttaacatc gtcctcggag
      241 cagtccgcgg caagtatcat accgtttgat tccactccgc acagcaccgc aggctttaag
      301 tttgcgacaa caacaacctt tttgcctaca aggtcctcgg gcgcatacca ttttgcgata
      361 ccgctcgcca cggttctgtc cttgcccgtg ccgtcatcga gcgtgagctt taacagcttt
      421 ttcgctttcg gaaccttttc gcacgccttg acctcggcca cgcgaagctc aacctttgcg
      481 aaatcgtcaa tgcctatctc ggcaacgccc tcaagcgaat ttatcatttt cttttcggcc
      541 ttctgctgct ccttctgctt ggcggtcagc tcctctatga gcttttcacc gtctatacgg
      601 ttaaacagcg gcgtgggagt tcccactttt tcacccgact ttaacgcgcc gaaggctttg
      661 aggctctcgt atgagcgttc gtttgtgcct atctgctcaa atattttttc tgccgtatcg
      721 ggcataaacg gagttaagag caccgcaagg aaacgtatac cctcaagcaa attgtaaagc
      781 accgtgccga gtctgccctt tttgctctcg tcctttgcga gtgcccaggg cgtagtctcg
      841 tcgatatact tattaaggcg gcgtgcaaga gtgagaatgt tctccgtagc gtcgcttatc
      901 ttgtattcgt caaagcacct gtcaacctca gccacggtct tcaaagcaag cgacgatatt
      961 tcgtcgtcaa gcggctcttt gtcggtagga gcgtcaatta cgccgtcaaa atatttattc
     1021 tgcatggcaa cggttctgtt aacaaggttg cctatggtgt tagcaaggtc ggaattgtat
     1081 ctctcgataa gcgattcgta cgttatggag ccgtcctgcg cgtgcggcat ctcggagaga
     1141 agatagtaac gcaccgcgtc aacgccgaaa tcctttacaa ggtcgtcggc ataaatgacg
     1201 tttccgcggg attttgacat tttatcctcg ccgaaaagca gccacgggtg accgtatacc
     1261 tgcttcggaa gaggttcgcc gagagccata agcataatcg gccaataaat ggtgtggaat
     1321 ctcacaatat ccttgcctat gatatgaaca tcggcaggcc aatacttttt atacatatcg
     1381 cttgagccgt cgggatcgta gccgagcgcc gtgatatagt ttgaaagtgc gtctatccaa
     1441 acatagatga cgtgattcgg gtcaaacgtt acgggaatac cccatttaaa tgacgttctc
     1501 gaaacgcaca aatcctgaag tccgggttta ataaagttgt ttagcatctc ttttttgcga
     1561 gcttcgggat aaatgaaatc gtcgtggctc tcgatgtagt cctcaagctg cttctgatat
     1621 ttggaaaggc gcaggaaata cgcctcctct tttgcctttt taacctctct gccgcagtcg
     1681 gggcacttgc cgtctacaag ctggctctcg gtccaaaagc tctcgcacgg ggtgcagtac
     1741 attccctcat actcgctctt gtatatatcg ccctgttcat agagcttttt gaaaatcttt
     1801 gcaactattt tttcgtggta cgggtcagtg gttcttatga acttatcata tgtggtgtta
     1861 agtacgtcgc aaatggcgcg tatttcgccc gacaccttat cgacatactc cttaggagac
     1921 acaccggcct cttttgcata ttcttcaatc ttctgaccgt gctcgtccgt tcccgtaagg
     1981 aaaaacacat cgtagccctg aagtcttttg tatctcgcta ttgcatcggt caaaactatt
     2041 tcatagctgt tgccgatatg cggcttacgc gaagtgtacg caatagccgt agttatatag
     2101 aatttaggtt tttcgctcaa aatacaaacc cctttcaaat aatatttata taagaccgtt
     2161 ttgctgcttt gcggcggtaa cggtgttttt aagaagtgtc gctattgtca ttgggcctac
     2221 gccgcccgga acgggagtta tgtaagacgc ctttttgctt acctcggcaa aatccacgtc
     2281 gccgcagagc ttgccctcgc tgtttctgtc catacctacg tctataacca ccgcaccgtc
     2341 ctttaccata tcggcggtta caaagtacgg cttaccgacg gcggcaacaa gaatgtcggc
     2401 gcgacgtgta acctctttga gatttttcgt cttagagtgg caaatcgtaa cggtgccgtt
     2461 ctgctgaagc agcagcatag ccatcggctt acccacaata ttgcttctgc ctacaacgac
     2521 acattccttg ccgcagacgt cgatattgta atatttcagc atttccataa ttccggcagg
     2581 cgtacacggc aaaaacgtgt ggtcgcctat cataatatgc ccgacgtttt ctgcgctaaa
     2641 ggcgtcaacg tcctttttcg gcgagattgc ctttatcaca agctcgtccg aaagcccctt
     2701 cggcagcggc agctgacaaa gtatgccgtt tacctctctg tcattattaa gctcgtctat
     2761 aagtgcgagc agctccgcca tagacgtatc tgccggcaaa gcatattcgc gtgagattat
     2821 accgacggcc tcgcacgcct ttttcttatt gtttacgtat gtccttgagg cgggatcgtc
     2881 gcccactatt ataacagcaa ggcaaacgga aacgcctttt tcctttaatt caaggcactc
     2941 cttttttata ccgtccttca aaaatgcgga aacggcttta ccgtcaatta tcgtcgccat
     3001 cgtttttgcg ctccttttgt ctcatcatct cgcggcgttt ttccgcgctg attttatttt
     3061 tctttttgtc ctcattgccg tcctttaacg cgtccgattt ttcggcatcc tcgcgcacct
     3121 ctacgggtga aaacggtttg ggatgttttt tcagcgaaac aagctttgcc gtaagtatcg
     3181 ctaagcaaac gacagctata accattgaca aaagctgtga aacacgaaga tttgacgagc
     3241 ctatataaag actgtccgtg cgaagtccct cgaccannnn nnnnnnacgc cgtagcaaag
     3301 tatgagctgc ccgctgaatt tgtagaattt gcgacaaatg atatatatta ccaaaaagcc
     3361 caaaaggcac catacggatt catacaggaa cgtcgggtga acgggaacat tcggagacat
     3421 ctctatgccc tttgccctga acagagcctg atttgacact atgtaatcga caaccttgga
     3481 gcttgtcatg ccccacggca ttgtcgtatt tgtaccgaac gcctcttgat ttgcataatt
     3541 gccccatctg ccgatgccct ggcctatcaa aaagctcatt ccggcaaggt caagtagctt
     3601 taaaaagtcc attttatcga ccttacaggt tatggctgcg gcgattattc cgccgataag
     3661 tccgccgtag attgcaagcc cgccgttcca aattttcgga atttccgaaa gattttggct
     3721 gtaataatcc cactcaaagg ctacatagta aagccttgca cctataattc cgcctatcgt
     3781 tccgtacagc agcacgtcaa gcattttgtt taaatccatc ttccacttgt aagccattct
     3841 gccgccgaaa agcaccgcga gcaaaaagcc gaacgctata ataagcccgt agaatttaat
     3901 gctgaacgta gaagaaccga tattgaactc tacaaaggtc gaatacacat tgaacgtgtg
     3961 gtctattccg gtaaatttta ccgccgtgcc gtcggtcaaa aaagtcatat cttcttttct
     4021 cctttaattg ctctttataa ccgagagcac gcagttttcg gtgtcaacgg agcttaaaag
     4081 tctcttttca acatcttcct tggtgagatt ttcaagcacc tccattcccg agaaaacagt
     4141 ctctccgtta aaggtcgccg ataccatctc gttggcaata gactctatat cgttaaaatc
     4201 gaacaccgct ctgccataca gcttgcggcg tatgcgctcg aaatcctttt cgtccacgcc
     4261 gtccctttta agctcgttta tcttaccgga gatcagctct ctaacctttt cggggtcgcg
     4321 gctctcgccg gaatatatct ggcaggcata accgtagccc tcaaaaaatt caaagccgaa
     4381 tgcggtgttt ataagcccgt cggagagcat atcggaataa agctttgacg tcttgcccgc
     4441 aacaagctca agaattatat tactgcatat aacgtcttta agactgcgct ccggggtttt
     4501 aacatcctcc ttgtagccga gtgcaaaaac cggcgtggct acgggtaaaa actcctccac
     4561 gtagctttcg cagacctctt tcggctcctc gtggaatttt ctctcaatct ttttttcggg
     4621 ctgcttttta agcaccttgt cagctactga caaaaccgtg tcaacatcca cattgcccgc
     4681 gacgcagagc accatattcg aaagattgta aaaggtgccg tagcatttgt agagcagctc
     4741 cgccgttatg tgagatatag actccacggt tccggcaatg tctattctca cgggatgctt
     4801 tttgtacatt ccgcgaagca tgccgaagag cacctcccag gacggctcgt cctgacacat
     4861 tttaatttcc tgaccgatta tgccctgttc cttttcaact gtttcgggcg taaaatacgg
     4921 atgggttaca aagtcgagta aaatttcgag agagccctta aagtcgcccg agcatgaaaa
     4981 cagataacag gtcttatcaa agctcgtata tgcgttagcg cttgcgcccg ttgcggcgta
     5041 acgctgaaat gcgtcgagat cctcgctctc aaaaagcttg tgctcaagaa aatgcgctat
     5101 accctccggt acttcggtaa actctttttc gcccgaaagc ttaaactgcg tatctatgga
     5161 gccgtagcgc gtaccgaaca tggcatatga cgacgcatag ttcttttttg gatacacaag
     5221 aattttaagc cccgactgat gctttatttc gtaatagctc tcaccgagct tttcgttttt
     5281 gatttctgtg acgttattca tctgcgccgt cctccttatc cgttcccgca agcatataaa
     5341 cggtatcgag cgagagcgcg caagccgttt tctttacgtc gtcccttgtg accgcaagga
     5401 agttttctat tgcctcatcc ggcgttacta tatcgcccgt cagctgcgtg ccgtaatata
     5461 tgtcaag
//
LOCUS       DBMDVO010000009         5432 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6057_length_5432_cov_1.297693, whole genome shotgun sequence.
ACCESSION   DBMDVO010000009 DBMDVO010000000
VERSION     DBMDVO010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_5432_cov_1.297693"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /gene="aspS"
                     /locus_tag="ACZPE2_00265"
     CDS             <1..1386
                     /gene="aspS"
                     /locus_tag="ACZPE2_00265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1318311.1"
                     /translation="LDLRRPDLQKNILLRHKITKITRDYFDENGFIEIETPMLIRSTP
                     EGARDFLVPSRIHNGSFYALPQSPQLYKQLSMVAGFDRYMQIARCFRDEDLRADRQPE
                     FTQIDLEMSFVDMEDVLSIGEGYIKRVFKDAIGVDVPTPLPRITYNEAMNRYGSDKPD
                     TRYGMELIDITDEVSSSEFVVFKSATAEGGTVRAINAKNAVSALSRKEIDKLTDYVKG
                     IGAKGLAWIRMTDDGVSSSFAKFMTEDEMNAILKKTGAEKGDVILIVADKIKNHVLTV
                     LGALRQKVAKELDIIPEGKYNFLWITEFPFFEYDEELKQFVAMHHPFTAPMDECLEYL
                     ETDKAKVRAKCYDLVLNGIELSSGSIRITDPKLQSRIFDLLGLSKEEAYSKFGYLLDA
                     FRYGAPPHGGMGIGLDRLCMQMIGCDSLRDVIAYPKLQNASEPMTECPAPVDDEQLNE
                     LGIGVTHTEGE"
     gene            complement(1450..2874)
                     /gene="glgA"
                     /locus_tag="ACZPE2_00270"
     CDS             complement(1450..2874)
                     /gene="glgA"
                     /locus_tag="ACZPE2_00270"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR1318312.1"
                     /translation="MKVLYVASEALPFMASGGLGDVAGSLPQALRKRLIGCRVVMPLY
                     DGIKQELKDTMTFITSISVPVAWRRQYCGIFEAKYKGVIYYLLDNQYYFKRDGIYGYY
                     DDAERFAFFSRAVLEMIPYIDFKPDIIHCNDWQTALTPLYYSSMYASSPGYENIKTVF
                     TIHNIQYQGTYGKELINEVLGIPQSATSLIEYDGDVNFMKGAIETANRVTTVSPSYAS
                     EILDPWYSYGLDTILNERSWKLSGILNGIDTELYNPETDKDIFVNYSANDFRKKADNK
                     RALQELMNLPQRADVPLIGMVSRLVSHKGLDLVRAVLDEFLGGTDVQMVVLGSGEWQY
                     EQFFKDMAAKYPDKFALKLGFVPSLSKKIYAGTDMFLMPSKSEPCGLSQMIALRYGSV
                     PIVRETGGLRDTIRDSGDGEGNGFTFASYNAHDMLYTIRRAIEGYQNREGWSILVKRA
                     MECDNSWGKSANEYIRLYKDLLKE"
     gene            complement(2926..4044)
                     /gene="glgD"
                     /locus_tag="ACZPE2_00275"
     CDS             complement(2926..4044)
                     /gene="glgD"
                     /locus_tag="ACZPE2_00275"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR1318313.1"
                     /translation="MTGNNIVGIIYSNIYDECLSEMTGLRTMGSVPFAGRYRLIDFPL
                     SNMVNSGIEKVGVITKSNYQSLMDHLGTGKPWDLSRKTEGMFILPPFASAGNKSFYSG
                     RVEALKANMGFIARSTEEYILFADCNNIVNIDIDELMDYHTDKAADITIVYKHGKCPR
                     LQNNMAFSFDDDSKVKMVAMSPASDTIVDYSMNIILMKKSLLQRLVNDAVSMNHESFE
                     RDIIQSNVEKLRIYGFEAKGFSLTVDSMQSYFDANMQLLDPDNCKQLFNRERPIYTKV
                     RDDMPAIYGLGSSVKNSLVANGCIIDGEVENSILFRGVRVEKGAVVKNSILCQGSFVA
                     SGSSLNYTVTDKSVAITPNKNLSGAEDYPVYVGKGIVI"
     assembly_gap    4211..4220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4221..5263)
                     /locus_tag="ACZPE2_00280"
     CDS             complement(<4221..5263)
                     /locus_tag="ACZPE2_00280"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1318314.1"
                     /translation="MARKMECIAMLLAGGQGSRLGILTKKIAKPAVPYGGKYRIIDFP
                     LSNCVNSGISTVGVLTQYQPLELNDYIGNGQPWDLDRAQGGVHILSPYQQIKGSEWYK
                     GTANAIYQNINFIDRYNPEYVAVLSGDHIYKMDYSKMLDYHKEKGAACTIAMLEVPWE
                     EASRFGLMITDEDNAIKEFEEKPKNPRSNKASMGVYIFTWSKLRKYLIDDENNPESSN
                     DFGHDVIPAMHAAGEKLYAYLFDGYWKDVGTIDSLWEANLDLLNPKVDLDLSDPTWKI
                     YSKTPEAPPHYVSPEATVQNSMVAEGSQVYGELDFSILFSNVTVECGAVVRDSIVMPG
                     TVIKEGAVVQYAIV"
BASE COUNT         1358 a         1392 c         1264 g         1408 t
ORIGIN      
        1 cttgaccttc gcagacccga tttacagaaa aacatactgc ttagacataa aataactaaa
       61 ataacgcgtg actattttga cgagaacggc tttatcgaaa ttgaaacgcc gatgcttatc
      121 cgttccactc ccgagggcgc acgcgacttt cttgtaccgt caagaattca caacggcagc
      181 ttctatgcct tgccgcagtc gccgcagctt tacaagcagc tctccatggt tgccggcttt
      241 gacagatata tgcagatagc gcgctgcttc cgcgacgagg atctgcgcgc agaccgtcag
      301 cccgaattca cgcagataga ccttgaaatg tcctttgtcg atatggagga cgtgctttca
      361 ataggcgagg gctacatcaa gcgcgttttc aaggacgcca taggcgttga tgtgccgaca
      421 cctctgccga gaatcaccta caatgaggct atgaaccgtt acggctcgga caagcccgac
      481 acaagatacg gtatggagct tatcgatata acagacgagg tttcctcgtc cgaattcgtc
      541 gtattcaaaa gcgcaacagc cgagggcggc acagtcagag ccatcaacgc aaaaaatgcg
      601 gtatcggctc tctcgagaaa agaaattgac aagctcacgg attacgtaaa gggcataggc
      661 gcaaagggcc ttgcttggat tcgtatgaca gacgacggcg tttcctcatc ctttgcaaag
      721 tttatgacag aggacgaaat gaacgccata cttaaaaaga caggtgccga aaagggcgat
      781 gttatactga ttgtagccga taaaatcaaa aatcacgttc tcacggtgct cggcgcatta
      841 aggcaaaagg ttgccaaaga gctcgacatt atccccgagg gaaaatacaa tttcctgtgg
      901 attaccgagt tcccgttctt tgaatatgac gaggagctca agcagtttgt cgcaatgcac
      961 catcccttca ccgcaccgat ggacgagtgc ctcgagtacc tcgaaaccga caaagccaag
     1021 gttcgggcta agtgctacga ccttgtgctc aacggaattg agctttcgag cggctccata
     1081 agaattaccg accccaaatt gcagagcaga atttttgatt tgctcggcct ttccaaagaa
     1141 gaggcttaca gcaaattcgg ctatctgctt gacgctttcc gttacggcgc accgccgcac
     1201 ggcggaatgg gcataggtct tgacagactt tgcatgcaga tgataggctg cgacagcctc
     1261 agagacgtta tcgcgtatcc caagcttcaa aacgcgtcgg agcctatgac ggaatgtccc
     1321 gctccggttg acgacgagca attaaatgag ctcggcatcg gcgttacgca cacagagggc
     1381 gaataaaaag caaaaaatta cccgactcac attcggccat atgtgagtcg ggtttttatt
     1441 ttatacggat tactccttta gcaaatcctt gtaaagtctt atgtattcgt tagcggactt
     1501 gccccagctg ttgtcgcact ccatggcgcg ctttacgagt atgctccagc cctcacggtt
     1561 ctgatagccc tcaatcgcac ggcgtattgt gtagagcata tcgtgagcgt tgtagctcgc
     1621 aaatgtgaag ccgttgccct caccgtcgcc gctgtccctt atcgtgtcgc gcagaccgcc
     1681 cgtctcacgg actatcggca cagagccgta gcgcagagct atcatctgcg aaagtccgca
     1741 cggctcactc tttgacggca taaggaacat atcggtgcct gcataaatct tctttgaaag
     1801 tgacggaaca aatccgagct tcagagcgaa tttatcggga tatttcgccg ccatatcctt
     1861 aaagaactgt tcatactgcc actcgcccga gccgagaacg accatctgca catcggtacc
     1921 gcccaagaac tcgtcaagaa cggctcttac aaggtcaagt cccttgtgcg aaacaagcct
     1981 tgataccata ccgataagag gtacgtccgc tctctgcggc aaattcataa gctcctgaag
     2041 agccctcttg ttgtccgcct ttttgcggaa atcgttcgcc gaataattta caaagatgtc
     2101 cttatcggtt tcgggattat aaagctcggt gtctataccg ttgagtatgc ccgagagctt
     2161 ccatgaacgc tcgttgagaa ttgtgtcaag tccgtaggaa taccacgggt ccaaaatctc
     2221 actcgcataa gagggactta cggtagttac cctgtttgca gtctctatgg cacccttcat
     2281 aaagtttacg tcgccgtcat actcaataag cgacgttgcg ctctgcggta tgccgagaac
     2341 ctcgtttata agctccttgc cgtatgtgcc ctggtactgg atgttgtgta ttgtaaacac
     2401 ggtcttgatg ttttcatatc cggggctgct tgcatacatt gagctgtaat agagcggcgt
     2461 aagagccgtc tgccaatcgt tgcagtgaat tatatcgggc ttaaagtcta tatacggtat
     2521 catctcaagc accgcgcgcg agaagaatgc aaatctctcg gcatcgtcat aatagccgta
     2581 aattccgtcg cgcttaaaat aatactgatt atccaaaaga taatagataa cgcccttgta
     2641 cttagcctca aaaatgccgc aatactgcct gcgccatgcc acgggcacgg atatgctcgt
     2701 gataaacgtc atggtatctt taagttcctg cttaatgccg tcatagagcg gcataacaac
     2761 gcggcaccct ataagacgtt ttctgagagc ctgcggcaga ctgcctgcca catcgccgag
     2821 gccgcccgaa gccataaacg ggagtgcctc gctcgctaca tataatactt tcatttaaaa
     2881 tcgcctcaat caaaaatttg taaacggtaa actctgttcg ccgcgttaaa taactatgcc
     2941 cttgccgaca taaaccggat aatcctccgc gccggagagg tttttattag gcgttatcgc
     3001 aacgctcttg tcggtaacgg tgtagttgag agaagagccg gaggccacaa agctgccctg
     3061 acagagaatc gaatttttga ccaccgcgcc cttttccact cttacgccgc ggaaaagtat
     3121 ggagttttca acctcaccgt ctattatgca gccgtttgcg acgagcgaat ttttaacgct
     3181 tgagccgaga ccgtaaatag ccggcatatc atcgcgaacc tttgtgtata tggggcgctc
     3241 tctgttaaac aactgcttgc agttgtcggg gtcgagaagc tgcatattcg cgtcaaaata
     3301 gctctgcatg gagtcaacgg taagtgagaa gccctttgcc tcaaagccgt aaatgcgaag
     3361 cttttcgaca ttgctttgta tgatgtcgcg ctcaaacgac tcgtgattca tacttacggc
     3421 atcgtttaca agcctttgaa gaagagattt cttcataagt attatgttca tcgaataatc
     3481 aactatggta tcgcttgcgg gcgacattgc gaccattttc actttgctgt cgtcatcaaa
     3541 ggaaaacgcc atattgtttt gcagacgcgg acatttaccg tgcttataaa caatcgttat
     3601 atctgccgcc ttgtcggtat ggtaatccat aagctcgtca atgtcgatgt ttactatatt
     3661 attgcaatcg gcaaaaagta tgtactcctc ggtggagcgt gctataaagc ccatattcgc
     3721 cttgagagcc tcgactctgc cgctgtaaaa gctcttgttg cccgcggagg caaacggcgg
     3781 aagaataaac attccctcag tcttgcggga gaggtcccac ggcttgcctg ttccgagatg
     3841 gtccataagc gactgatagt tgctctttgt gataactccg accttttcta tgccgctgtt
     3901 taccatattg gaaagcggaa agtcgataag gcgatatctg ccggcaaacg gcacgctgcc
     3961 catggtgcga agccccgtca tctcgctgag acactcgtca tatatgtttg aataaatgat
     4021 tccgactatg ttatttccgg tcatttttaa gcccccttta cgtcttcgtc aaccattgcc
     4081 ttgggtgcca caactccgtt tttgcctacg gtaacaccgc ttccgactac ggctacgccc
     4141 cagtcgtcaa gattttccac atcctcgggt ctcgctccga caacggcatt ctcttcaatg
     4201 accgcgtttt nnnnnnnnnn actattgcat actgtaccac agcaccctct tttatgaccg
     4261 ttccgggcat cactatggaa tctcttacca cggcaccgca ctcaacggtg acattcgaga
     4321 acaaaatcga gaaatcaagc tcgccgtaaa cctgcgagcc ctcggcaacc attgaattct
     4381 gcacagtagc ctcgggtgaa acatagtgcg gaggcgcttc gggagttttt gaatagattt
     4441 tccaggtggg gtccgaaagg tcaaggtcaa ccttgggatt taaaaggtca aggtttgctt
     4501 cccaaaggga atcgatagtt ccgacgtcct tccagtagcc gtcaaagagg taagcgtaga
     4561 gcttttcgcc ggcggcgtgc attgccggta ttacgtcgtg accgaagtcg tttgacgact
     4621 ccggattatt ctcgtcgtct ataagatact tgcgaagctt tgaccacgtg aatatgtaaa
     4681 cgcccatgga agccttgttg cttctgggat ttttcggttt ttcctcaaat tcctttatgg
     4741 cgttgtcctc atcggtaatc ataaggccga agcgcgaagc ctcctcccac ggaacctcaa
     4801 gcattgcaat ggtgcaggcc gctccttttt ccttgtggta atcgagcatc tttgagtaat
     4861 ccatcttgta aatatggtcg cccgagagaa ccgccacata ttcgggatta tatctgtcga
     4921 taaagtttat gttttgataa atagcgtttg cggtgccctt gtaccactcc gagcccttta
     4981 tctgctgata aggcgagaga atatgaactc ctccctgcgc tcgatcgaga tcccacggct
     5041 gaccgttgcc gatgtagtcg ttaagctcaa gcggctggta ctgggtcaga acaccgacgg
     5101 tcgaaatgcc ggaattcaca cagtttgaaa gcggaaagtc gataattctg tacttaccgc
     5161 cgtagggaac tgcgggcttt gcaatcttct tggtcaaaat tccgagtctt gaaccctgac
     5221 ctcctgccag cagcatcgca atacattcca ttttgcgtgc catagattta tcctcctttt
     5281 aaaggcctta gcctctgcct tgcaccggac tggtacaaga ctaccttgtt ttttttatgt
     5341 atattgcaga catcggcggc aaatcaagag aaatgctttg ctcgaagccg tgcatattta
     5401 tcgattcggt ttttaattta cccttgtttc cc
//
LOCUS       DBMDVO010000010         5282 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6307_length_5282_cov_1.285273, whole genome shotgun sequence.
ACCESSION   DBMDVO010000010 DBMDVO010000000
VERSION     DBMDVO010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_5282_cov_1.285273"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="ACZPE2_00285"
     CDS             <1..609
                     /locus_tag="ACZPE2_00285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1318315.1"
                     /translation="IWDTAGEDYLKQCGKVPRENLCEVLKPKSLSGAAEYLKKNYDLP
                     LSVNEIVDGVNKTVGDFYLCEAQPKNDVREFLTALREINVRMCITTAAERKLTEGALK
                     RCGLFGFFEKIFTCGEVGHDKNDPYIYCEALSFLGTKKDETLVFEDAYHAAKTAKGAG
                     FYVAAVYDSREKAPEKMKAVSDIYLNNFLFPSDFINDIKKIK"
     gene            690..1502
                     /gene="thiD"
                     /locus_tag="ACZPE2_00290"
     CDS             690..1502
                     /gene="thiD"
                     /locus_tag="ACZPE2_00290"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1318316.1"
                     /translation="MKTALSIAGSDCSGGAGIQADLKTMTMNGVYAMSAIAALTAQNT
                     TGVTDISEVSPEFLKEQIDAVFTDIRPDAVKTGMIPTAALIRVTAERLKFYDAQNIIV
                     DPVMVATSGSRLMESPAVAVMKKELLPISTLVTPNIPEAAVLSGMEIKDKDDMEKAAA
                     KIHSECGCAVLLKGGHSIEDASDLLYNENESFWFYGKRIANPNTHGTGCTLSSAIAAN
                     LAKGYDLKASVKLAKDYISEALAFMLDLGKGNGPMNHAFMLSGKYAAKSSGE"
     gene            1565..2875
                     /gene="thiC"
                     /locus_tag="ACZPE2_00295"
     CDS             1565..2875
                     /gene="thiC"
                     /locus_tag="ACZPE2_00295"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR1318317.1"
                     /translation="MKTEAYATQMDAARKGIITDELKAVAEKEHMTTDELLPLVASGK
                     VVIPANKRHKCLSPEGVGSMLRTKINVNLGVSRDCKDYDIEMQKVLSAVNMGAEAIMD
                     LSSHGNTQPFRQKLTHECPAMIGTVPVYDSVIHYQRDLDTLTAKDFIDVIRLHAEDGV
                     DFVTLHCGITRKTIDQIRKHKRKMNIVSRGGSLVFAWMCMTGEENPFYEYYDEILDIC
                     REYDVTISLGDACRPGCLADATDVCQIEELVRLGELTKRAWEKDVQVMVEGPGHVPMD
                     QIAANMKVQQTICMGAPFYVLGPLVTDIAPGYDHITAAIGGAMAAMSGAAFLCYVTPA
                     EHLALPNLDDVKQGIIASRIAAHAADIAKGVRGARDIDDKMGDARRALDWEAQWKCAI
                     DPETAKAIRDDRSPEHDDTCSMCGKFCAVRSMNKALAGEHIDIL"
     gene            complement(2951..3082)
                     /locus_tag="ACZPE2_00300"
     CDS             complement(2951..3082)
                     /locus_tag="ACZPE2_00300"
                     /inference="COORDINATES: protein motif:HMM:NF021546.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR1318318.1"
                     /translation="MIYFVVNRYQVGKMKDYVHGIDPTAYIIMSEVADIFSNNIDKG"
     gene            complement(3212..3789)
                     /locus_tag="ACZPE2_00305"
                     /pseudo
     CDS             complement(3212..3789)
                     /locus_tag="ACZPE2_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014627.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
     gene            complement(4211..5023)
                     /locus_tag="ACZPE2_00310"
     CDS             complement(4211..5023)
                     /locus_tag="ACZPE2_00310"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014093984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1318319.1"
                     /translation="MAYLELRDVYFEYPSGHVAVEGVSISADLGEKIAIIGENGAGKT
                     TTAKLMNGLLKPTKGEVLINGVSTKGKTTATIARKVAYVFQNPDDQIFNQEVLAEISY
                     TPRYFKVDEETVNKRLKKAVKLTGLKPYINENPYNLSYSYRKFVTIAAAIAADPDVFV
                     FDEPTACQDRHGIRLLSGIINALSSEGKLVITITHDMEFVAENFDRTVCMADKHILMD
                     DKTNRILTDDSLMEKAALKKPNVSYLASEMGFEGVITSEDFLAALTENSKRL"
     gene            complement(5010..>5282)
                     /locus_tag="ACZPE2_00315"
     CDS             complement(5010..>5282)
                     /locus_tag="ACZPE2_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318320.1"
                     /translation="KMNLIAEYADHIVYLNDGTVLLDGSPEDVLSSEILYEKNLDMPY
                     VAVLGFQARSRGLFIDKIPTTVDEALKIFTECVQKSKGGSDGCGLS"
BASE COUNT         1545 a         1279 c         1169 g         1289 t
ORIGIN      
        1 atatgggaca ccgcaggaga agattattta aagcaatgcg gcaaagtccc gagagaaaac
       61 ctctgtgaag tgcttaagcc caagagcctt tcgggggcag cagaatatct caaaaaaaat
      121 tacgatttac ccttatctgt aaacgaaata gttgacggcg taaacaaaac ggtgggcgac
      181 ttctatctct gcgaagcaca gccgaagaac gacgtacgag aatttctaac cgctcttcgc
      241 gaaattaacg tgcgtatgtg cattacgaca gccgcggaac gaaagctcac agagggcgca
      301 cttaaaagat gcggcctttt tggatttttc gaaaaaatat tcacttgcgg agaagtcggg
      361 cacgacaaaa acgacccgta catatattgt gaggcactgt cgtttctcgg cacaaaaaaa
      421 gacgaaactc tcgtctttga ggacgcatat cacgccgcaa agacggcaaa gggagcgggt
      481 ttttatgtgg cagcggtcta tgacagccgc gaaaaagctc ccgaaaagat gaaagccgta
      541 tcggacattt acttaaacaa ctttcttttc ccgagcgact ttataaatga tataaaaaag
      601 ataaaataac aaaacagcgg caaaccgggg gcaccacctt ttgtcgctct ataaaaataa
      661 tgctaagctt ataaaaaacg gaggcaaata tgaaaacagc attatcaatc gcaggcagtg
      721 attgcagcgg aggcgccggt attcaggccg atctgaaaac gatgactatg aacggagttt
      781 atgccatgag cgccattgcg gcgctgacgg cacagaacac caccggcgta acagacatct
      841 cggaggtatc tccggaattt ctcaaggaac agattgacgc ggtattcacg gatatacgac
      901 ccgatgcagt taagacgggt atgattccta cggcagcgtt aattcgcgta acagccgaaa
      961 ggctgaaatt ctacgatgct caaaacatca tagtggatcc ggttatggtt gcgacctccg
     1021 gctcaaggct catggaatct ccggcagttg ccgtgatgaa aaaggaactt ttaccgattt
     1081 ctacactcgt aacgcccaat attcccgaag cagccgtgct atccggcatg gaaattaagg
     1141 acaaggacga tatggaaaag gccgcagcaa aaattcattc ggaatgcggc tgcgccgtgc
     1201 ttttaaaggg cggacacagc attgaggacg caagcgacct tctctataac gaaaacgaga
     1261 gcttttggtt ctacggaaag cgcattgcca atcccaacac ccacggcacc ggctgcacgc
     1321 tgtcaagcgc aatagccgcc aacctcgcca agggatacga tttaaaagct tccgtaaagc
     1381 ttgccaagga ttacatctcg gaggcactcg cctttatgct cgatttaggc aagggaaacg
     1441 gtcctatgaa tcacgctttc atgctttccg gcaaatacgc cgcaaaaagc agcggagaat
     1501 aaaaagtcaa agttatgagc ggctctgccg ttcgacgatc aacctaatac aaaggagaac
     1561 aaaaatgaaa acagaagctt acgcaacaca gatggacgcc gcaagaaaag gcattattac
     1621 cgatgagctg aaggcagtag ccgaaaaaga gcacatgaca acagatgagc tattacccct
     1681 cgtagcctca ggtaaggtcg taatacccgc caacaagcgt cacaagtgcc tgtcgcccga
     1741 gggcgtcggc tcaatgctcc gcacaaaaat aaatgtaaat ctcggcgttt cgagagattg
     1801 caaggactat gacattgaga tgcaaaaggt tctctcggca gttaatatgg gtgctgaagc
     1861 tataatggat ctctcaagcc acggcaacac acagccgttc aggcagaaac ttacgcacga
     1921 atgtcctgct atgataggca cggtgcccgt ttacgacagc gtaattcact atcagagaga
     1981 ccttgacact ctgaccgcaa aggactttat tgacgttata agacttcatg cagaggacgg
     2041 agttgacttt gtaacgctcc actgcggaat cacaagaaaa acaattgacc aaatacgtaa
     2101 gcacaaaagg aaaatgaaca tagtttcgcg cgggggctca ctcgtttttg catggatgtg
     2161 tatgacaggt gaagaaaacc cgttttacga atactatgat gaaatacttg atatatgccg
     2221 cgaatatgat gttacaatct ctctcggcga cgcctgccgc cccggctgtc ttgccgacgc
     2281 gaccgacgta tgtcaaatcg aagagcttgt acgtctcggc gaattaacaa aaagagcttg
     2341 ggaaaaggac gttcaggtta tggttgaggg ccccggacac gttccgatgg accagatagc
     2401 tgccaatatg aaggttcagc agacaatatg catgggtgct cccttctacg ttctcgggcc
     2461 gctcgtaacc gatatagcgc ccggatatga ccacataacg gcagcaatcg gcggagccat
     2521 ggcggcaatg agcggcgcgg ctttcctctg ctatgtaact ccggccgagc atctcgctct
     2581 cccgaatctc gacgacgtta agcagggcat catcgcaagc cgtatagcgg ctcatgcggc
     2641 ggatattgcc aagggcgtcc gcggagcacg tgacattgac gacaaaatgg gcgacgcaag
     2701 acgtgcactt gattgggaag cccaatggaa atgtgccatt gaccccgaaa cggctaaagc
     2761 aatacgtgac gaccgctccc ccgagcacga cgacacctgc tcaatgtgcg gtaagttctg
     2821 tgccgtccgc agtatgaata aggcactcgc cggcgagcat atagacatac tgtaaaaaac
     2881 actcattaag caaaaacgag ctgtaacggt taaaaccttt acagctcgtt tcgtttttat
     2941 ttgttaaata tcagccttta tcaatgttat ttgagaaaat atcggcaacc tcactcatta
     3001 tgatatacgc cgtcgggtct atgccgtgaa cgtagtcttt cattttacct acctgatacc
     3061 tgttaactac gaaataaatc atcgccttat cacgatttga atatccgccc tttgcattta
     3121 gccttgtaag gccgctgcca aacgcctcgc tcaagacctc acatatctct tcgggtcgtt
     3181 cggttataat gacggcacac tttgagcggt ctataccctc gactataaaa tcgaccgttt
     3241 tcaacgccgc cgaatacgta actatggaat aaagcggaag cacccagctt ttaagcacga
     3301 ttccgcaaat gacatacaaa aggaaattat atatcataac gaatgtgccg acggtaattc
     3361 cgagccgctt tgcaaaaatg accgccataa cctcaatgcc gtccattgcc cctccgtaac
     3421 ggatagcaag accgcttccg ataccggaaa ggacgccgcc gaacagcgca caaagcaaca
     3481 aatcggttcc ggcgagcggc gatgctatgc tcacatctac cggaaacaca tcggtaatca
     3541 gccacgcaaa cagcgaataa acgccaaccg cataaatcga ataaaacgta aagcctgcgc
     3601 cttgcttttt aagtccaaac aaaaaagcgg cacatttagg attaaaagaa acagagaaag
     3661 agtcaaattc ggcggcgtaa tctgctcaag cagcattgat gttccggaaa ttccgctgtc
     3721 gtacagcttg accggggtca taaaaatcgt aatgccgaaa gcgtttatca ttccggctat
     3781 cgtaagcatc aaaatatttt taaaagctct tttaaatcga atgtttcggg ggtgtttcac
     3841 ttgacaagca ctcctttaat aaacataggt cccgcaagcc gccatggcaa ggtgataagc
     3901 ttaacattaa atccgccgcc tcttctgtat tctacgcaag cttatgcaca ttcgagatta
     3961 tcaattagat ttttcagtat aaattatata taaatcggag atcaaagtca atcggcagta
     4021 atttacgaag tgtcatctca cacagcacgc aaaaatcagc cctttaagaa acatttattt
     4081 ctcaaagggc tgattaactg aattaaatta agacagtgct tttttgagac ttgttccgaa
     4141 aatcaatcgc aataaaatcg attcataagc gtcgccgccg acaaatcttt cggcacttca
     4201 tcatctccgc tcaaagtcgc tttgaatttt cggtaagagc cgcaagaaaa tcttcacttg
     4261 taataacacc ttcaaatccc atttcggatg cgagatatga cacattcggt tttttcaatg
     4321 cggccttctc cataagcgag tcatcggtaa ggattctgtt cgttttatcg tccataagta
     4381 tatgcttgtc tgccatacac acggttctgt cgaagttttc ggcaacaaat tccatatcat
     4441 gtgtaatggt aattacaagc tttccctcgg aggacagtgc attgataatt ccggacaata
     4501 gtcttatgcc gtgtctgtcc tgacaagcgg tcggctcatc aaaaacaaat acatccgggt
     4561 cggccgctat tgccgcggct atcgtcacaa actttctgta tgaatacgaa agattgtacg
     4621 gattttcatt gatatacggc ttgagccccg tcaatttaac cgcttttttt aatctcttgt
     4681 tcaccgtttc ttcatccact ttaaagtatc tcggcgtgta tgaaatttcg gccagtacct
     4741 cctgattaaa aatttggtcg tcggggtttt gaaatacgta agccactttt cgcgcaatcg
     4801 tcgcggtcgt tttgcctttt gtcgatacac cgtttataag cacttcaccc tttgtgggct
     4861 taagcagacc gttcataagc tttgcggttg ttgtttttcc ggctccgttt tcgcctataa
     4921 tcgctatttt ttcgcctaaa tccgcagaga tgcttacgcc ttcaacggca acatgcccgc
     4981 ttggatattc aaaataaaca tctctcaatt caagataagc cacagccgtc acttcctcct
     5041 ttggattttt gcacacattc ggtaaatatt ttcagagctt catccaccgt tgtcgggatt
     5101 ttgtcgataa aaagtcctct gcttcttgcc tgaaatccga gaacggcaac atagggcata
     5161 tcaagatttt tttcataaag gatttcgctt gacaatacgt cctccggaga tccgtcaagc
     5221 agcaccgtgc cgtcattgag atatactata tggtctgcat attcggctat cagattcatc
     5281 tt
//
LOCUS       DBMDVO010000011         5251 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6368_length_5251_cov_1.634369, whole genome shotgun sequence.
ACCESSION   DBMDVO010000011 DBMDVO010000000
VERSION     DBMDVO010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6368_length_5251_cov_1.634369"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="ACZPE2_00320"
     CDS             <1..542
                     /locus_tag="ACZPE2_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR1318321.1"
                     /translation="VAVIVIALIGSIAGSYNNLVKLRENVTSAQSVVETQLQRRADLI
                     PNLVNTVKGYASHEEDVFTAVADARTALSGAKTVDELNSAQSQLDSAVSRLLAIAESY
                     PDLKANENFINLQDELAGTENRISVARQDYNDAAKEYNTKIQSFPASIIAGLFHFEKA
                     DYFTATAEAATVPAVDFGK"
     gene            546..1457
                     /locus_tag="ACZPE2_00325"
     CDS             546..1457
                     /locus_tag="ACZPE2_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1318322.1"
                     /translation="MKKTIGAGALLVSLLIVLLTALPAAAISEHPLPASSFFAPSFAD
                     DNKMTTQSQDDYPSPTSDFFVNDFASCLTDEDKSTIQALGEELYKKTKAQVVVVTVQS
                     LGGRNIESYSIGLAREWGIGDKDDDSGVLLLLSTGDRQVRIEVGKGLEGSLTDGKSGR
                     ILDSYAVPYLKNSDYSTGLRECYKKLVEEVYSEYGVEGYTSIDDTDIGDYSSSDSGNE
                     KIDLAVIIIITVLVLITLILPGRIIRKAIYHGGGDHHGDGGFYGGGFYGGGFGGHSSG
                     GFSGGSSSGGFSGGGGGFSGGGSSRGF"
     gene            1587..>1884
                     /locus_tag="ACZPE2_00330"
     CDS             1587..>1884
                     /locus_tag="ACZPE2_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318323.1"
                     /translation="MRMYDIIDNKKHGKELSFDEIKFFVDGFTDGSVPDYQASALLMA
                     ICLKGMSDSEISNLTLCMAQSGDMNDMSKIDGVSCDKHSTGGVGDKTSLIVAPIA"
     assembly_gap    1885..1894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1895..2899
                     /locus_tag="ACZPE2_00335"
     CDS             <1895..2899
                     /locus_tag="ACZPE2_00335"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR1318324.1"
                     /translation="ASCGLKVAKMSGRGLGHTGGTVDKLESIPGYNTAMASDSFFKQV
                     NKIGISLIGQTGNLAPADKKLYALRDVTATVDDRALISASIMSKKLAAGDKNIVLDVK
                     CGSGAFMKNEKDAAALAKTMVGIGKGCGRNIIAVITNMDEPLGRNVGNALEVIEAVDV
                     LRGNGDKSLRELSVYLAANMLSLSFQRDIDECIIEATNALDSGRAFDKFRELVEAQGG
                     DVDYINDTSLFKKDKCVRDILSPCDGYIFSADTGKIGEAAVILGAGREKKDDKIDMAA
                     GIVLRKKTGERVNKGDALATLYTSCEARADNAEKLYLNAIKIADNPPEKKKLIYGTIK
                     "
     gene            2952..3878
                     /locus_tag="ACZPE2_00340"
     CDS             2952..3878
                     /locus_tag="ACZPE2_00340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1318325.1"
                     /translation="MADENGGIFMPKKYNTIIIGGGTAGLTAAIYTARQNKSVAVFES
                     GIFGGQIADSPRIENYPGFCEISGEEFSDRLLKQALKLGAELIKKRVAAIKLEGKDKY
                     VISADGEKTQCDKIIIAVGTRPRPLYLENERELLGRGISYCAVCDGAFFKSKTVAAVG
                     GGSSALQSARLLAELCKKVYVIHRRNEFRGEQRLCEELKSFHNIEFLLNSEIKKINGK
                     TRLESVLVKNNLSGEETLLPLDGLFVAIGKEPQNEIFAEVTELDENGYIKADESCKTS
                     TDGIYAAGDCRTKAFRQLTTAAADGTVAALSP"
     gene            4156..4845
                     /locus_tag="ACZPE2_00345"
     CDS             4156..4845
                     /locus_tag="ACZPE2_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016365.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR1318326.1"
                     /translation="MKLIGAAVNCGAVFLGSLLGLLFKKGIPERVQKSLNYALSLCVL
                     YIGIKGSLRGENTLIAALSLAIGTIIGELIDIDRHLNKFGDFLQKKLSRNTENSSFGE
                     GFVNATLFTCVGAMAIVGAIEAGMQGSFSTYYAKALLDCFFVMIMTTTFGIGCFFSGI
                     IVFVYEAILTLGAGFLSSFLSDSMINEMSCVGSVLIIAIALNTMKVTKIKVANLLPSA
                     FMPLLLCLFIH"
     gene            4907..>5251
                     /locus_tag="ACZPE2_00350"
     CDS             4907..>5251
                     /locus_tag="ACZPE2_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1318327.1"
                     /translation="MKIIVAGDGKVGATLTRQLTAEGYDITLIDSNKDVLSSSMEQFD
                     IMTVHGNCATKDTLLKAGITDTDLIIAATSADEVNLLCCITAHGLNKNLHTIARIRNP
                     EYSSQIYDMRDMY"
BASE COUNT         1460 a         1202 c         1326 g         1253 t
ORIGIN      
        1 ttgttgccgt tatagtcatt gcacttatcg gcagcattgc cggcagctac aacaatctcg
       61 taaagctgcg tgaaaacgtg acgagcgcgc agtccgtggt tgaaacacag cttcagcgcc
      121 gcgcggacct tataccaaac cttgtaaaca ccgtaaaggg ctacgcttct catgaggagg
      181 acgtttttac ggcagttgca gacgctcgca cggcactttc gggtgccaag acggttgacg
      241 agctgaacag tgctcagagt cagcttgaca gtgccgtatc acgacttttg gcgatagccg
      301 aaagctatcc cgatctcaag gcaaatgaga attttataaa tcttcaggat gagcttgcag
      361 gcaccgaaaa ccgtatttcc gttgcgcgcc aggactataa cgacgccgca aaggaataca
      421 atacaaaaat tcaatcgttc cccgcatcga taattgcggg actcttccat tttgaaaagg
      481 cggattattt caccgccaca gccgaagctg caactgttcc cgctgtggat ttcggtaaat
      541 aagtcatgaa aaagacgata ggtgcaggcg cactgcttgt ttcacttttg attgttttgc
      601 tgacggctct gccggcagcc gcaataagcg agcatccgtt gccggcaagc agttttttcg
      661 ccccaagctt tgccgatgac aacaaaatga ccacacaatc tcaagacgat tacccgtctc
      721 ccacaagtga ctttttcgtc aacgattttg cctcgtgtct caccgatgag gacaaaagca
      781 ccatacaagc tctcggcgag gagctgtaca aaaagacaaa ggcgcaggta gtagttgtca
      841 cggttcaaag cctcggcggc agaaatattg aaagctattc gataggactt gcgcgtgaat
      901 ggggcatagg cgataaggac gatgattccg gcgtattgct gctgttatcc acgggcgaca
      961 gacaggtgcg aattgaagtc ggaaagggtc ttgagggctc tctcaccgac ggtaaatcgg
     1021 gacgaattct cgattcctat gcagttccgt acctcaaaaa cagcgactat tccacgggtc
     1081 ttcgcgagtg ctacaaaaag ctcgtcgagg aggtttattc cgaatacggt gtagagggct
     1141 atacgagcat tgacgacacg gacatcggcg attactcctc atcagattcc gggaacgaaa
     1201 aaatcgacct cgccgttatt attataataa cggttctggt actgattacc ttgattttac
     1261 ccggccgcat aattcgcaaa gcaatttatc acggcggcgg cgatcatcac ggcgacggcg
     1321 gcttttacgg aggcggcttt tacggaggcg gcttcggcgg acattcatcc ggagggttct
     1381 cgggaggctc gtcctcggga ggattttcgg gcggaggcgg cggcttctcc ggcggaggaa
     1441 gctcacgcgg attctaatat ttcacacata aagctgtcgg agcagggcct aatccttttc
     1501 ggcagctttt attttcccct gcttttgctt ttctgcattg ccgcaaggtt tataagcaat
     1561 caaattaaaa gaaaggtcgt ggcgaaatga gaatgtatga catcatcgat aacaagaagc
     1621 acgggaaaga gctgtccttt gacgaaatca agttctttgt cgacggattt acggacggct
     1681 cggtaccgga ctatcaagct tcggcgctgc ttatggccat atgccttaaa ggtatgagtg
     1741 acagcgaaat atcaaatctc accttatgta tggcgcagtc gggcgatatg aacgatatga
     1801 gcaaaataga cggcgtgtcc tgcgataagc actcaaccgg cggcgtcggt gataaaacct
     1861 cacttatagt cgctcccatt gccgnnnnnn nnnngcaagc tgcggtctga aggttgccaa
     1921 aatgagcggc agaggtctcg gccacacggg cggtacggtt gataagctgg agtctattcc
     1981 cggctacaac acagcaatgg caagcgacag cttttttaaa caggtaaaca aaatcggaat
     2041 ttctctgata ggtcagaccg gaaacctcgc acctgccgac aaaaagcttt acgcacttcg
     2101 cgatgtaaca gccaccgtgg atgacagggc tcttatctcg gcgagcatta tgagcaaaaa
     2161 gcttgcggca ggcgacaaaa atatagtcct cgatgtgaaa tgcggaagcg gcgcatttat
     2221 gaaaaatgaa aaggacgccg cggctctcgc aaaaacaatg gtcggcatag gcaaaggctg
     2281 cggcagaaac atcatagccg ttataacaaa tatggacgag ccgctcggca gaaatgtcgg
     2341 aaatgcgctt gaggttatcg aggcagtcga cgttttacgc ggtaacggtg acaagagcct
     2401 tcgcgaatta tctgtttatc tcgccgcaaa tatgctgtca ctctcattcc aaagggatat
     2461 agacgagtgt attatagaag cgacaaatgc gctcgacagc ggcagagcct ttgataaatt
     2521 ccgtgagctc gttgaggctc agggcggaga cgtcgattat ataaatgata caagcctctt
     2581 caaaaaagac aaatgcgttc gcgacatatt atcgccgtgc gacggataca tcttttcggc
     2641 ggacaccgga aagataggcg aagccgccgt gatactcggc gcaggcagag agaaaaagga
     2701 cgacaaaatc gatatggctg cgggaattgt actccgcaaa aagaccggcg aaagagttaa
     2761 taagggcgac gctctggcga cgctttacac ctcctgcgaa gcaagggccg acaatgccga
     2821 aaagctgtat ttaaatgcaa ttaaaatagc cgacaatccg cccgaaaaga aaaagcttat
     2881 atacggtaca attaaataat actttcccct cccggtttcg gataaaaccg caatttcgcg
     2941 gcaaactcat tatggcagac gaaaacgggg ggatttttat gccgaaaaaa tacaatacaa
     3001 ttataatcgg cggcggaacg gcaggtctta ccgcggcaat ttacaccgct cgccaaaata
     3061 agagtgtcgc ggtttttgaa agcggaatat tcggcggtca gatagccgat tcgccgagaa
     3121 tcgaaaacta cccgggcttt tgcgaaatca gcggtgaaga attttctgac aggctgttaa
     3181 agcaggcgtt aaagctcggc gcagagctta ttaaaaaaag agtagccgca attaagcttg
     3241 agggtaagga caaatatgtc atttctgctg acggcgaaaa aactcaatgc gataaaatta
     3301 taattgcggt cggtacaaga ccgcgtccgc tttatcttga aaacgaacgt gagcttttgg
     3361 gcagaggcat ttcatactgc gcggtttgcg acggtgcatt tttcaaaagt aaaacggttg
     3421 ccgcggtcgg cggcggcagc tcggctctgc aaagcgcaag gctgctggca gagctttgca
     3481 aaaaggtgta tgtgattcac aggcggaatg aatttcgcgg cgagcaaagg ctctgcgaag
     3541 agttaaagtc ctttcataac atcgagtttt tgttaaacag cgaaataaaa aagataaacg
     3601 gcaagacgag gctcgaaagc gttttagtta aaaataatct aagtggtgaa gaaacgcttc
     3661 ttccgcttga cgggctcttt gtcgcaatag gcaaggagcc gcaaaacgaa atatttgccg
     3721 aggtcacaga gcttgatgaa aacggataca tcaaggcgga cgagagctgt aaaacaagca
     3781 cggacggaat ttacgccgca ggcgactgcc gcacaaaggc atttcgtcag cttacgactg
     3841 cggcggcgga cggaacagtt gcggcgttgt cgccgtaatt accctgccga ttttcaaatt
     3901 acgaaacaca gcgtaaaaaa actaaaaatc cacacgcgtc gctgagcgtt tataaggcag
     3961 ttttgcttcg cggcttcaca gccgtacatt ttttatgcga aaccgatact tccttatgaa
     4021 cgctctacct tattattgat acacggcggc ttttgtgtta ttatagtcgc cgtaattaaa
     4081 attaaggtaa agagtgcccg tctctgcggc atttcgtcgg tttcggcgcg gcacggcaac
     4141 aaataaggtg aaaaaatgaa actcataggc gcagcagtaa actgcggagc tgttttttta
     4201 ggctctcttt taggtctgct gtttaaaaag ggtattccgg aacgcgtgca aaaaagtctt
     4261 aactatgcgc tgtcgctgtg cgtactctac ataggaatca agggttcgct tagaggagaa
     4321 aatacattga ttgccgccct gtcgctcgcg ataggaacaa taataggcga gctcattgac
     4381 attgacaggc atttaaacaa attcggcgac tttttgcaaa agaagctgag ccgcaatacc
     4441 gaaaattcat cgttcggcga gggctttgta aatgccacgc ttttcacctg cgtcggagct
     4501 atggcaatag tcggcgcgat tgaagccggt atgcagggca gcttttcaac atactatgca
     4561 aaagctctgc tcgactgctt ttttgtgatg ataatgacta ccacattcgg aataggctgt
     4621 ttcttctccg gaattatagt ttttgtctat gaagcgattt tgacgctcgg tgccggcttt
     4681 ctctcgtcat ttctctccga cagtatgata aacgagatgt catgcgtcgg ctccgtgctt
     4741 ataatagcca tagctctcaa cacaatgaag gtcacaaaaa taaaggttgc aaacctgctg
     4801 ccgtcggcat ttatgccgct cttactgtgc ctttttattc attgactttt acttttttta
     4861 tgctatatta tatgtggtta tatatgattt tttgggggaa ttgtaattga agattattgt
     4921 tgcaggtgac ggtaaggtcg gtgccacgct tacacgccag ctgaccgcag agggatatga
     4981 cattactctt atcgactcaa acaaggatgt tttgagctca agcatggagc aattcgacat
     5041 aatgaccgta cacggcaact gtgccaccaa agacacgctg ctgaaggcgg gaattacaga
     5101 caccgacctc ataatagccg ccacaagcgc ggacgaggta aatcttttgt gctgcattac
     5161 cgctcacgga cttaacaaga acctgcacac aatagcgcga atccgcaatc ccgaatacag
     5221 cagtcaaatc tatgatatgc gcgatatgta t
//
LOCUS       DBMDVO010000012         5218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6446_length_5218_cov_1.290991, whole genome shotgun sequence.
ACCESSION   DBMDVO010000012 DBMDVO010000000
VERSION     DBMDVO010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_5218_cov_1.290991"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(261..1592)
                     /gene="gdhA"
                     /locus_tag="ACZPE2_00355"
     CDS             complement(261..1592)
                     /gene="gdhA"
                     /locus_tag="ACZPE2_00355"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR1318328.1"
                     /translation="MSYIDEVLNRTTTRYDYQPEFCQAVTEVLKSIEPAVERNPKYQK
                     AALLERLVAPEKATVFRVPWVDDNGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVNMSI
                     IKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDYEIMRFCQCFMTELYKVIGPNSD
                     VPAGDIGVGGREIGYLFGQYKKITGRHEGVLTGKGLSYGGSLARTEATGYGLIYLVEE
                     MLKNHGNSIEGKTVAVSGSGNVAIYAIEKAQTFGAKVVTASDSSGYVYDKDGIDIALL
                     KQVKEQERARIVRYTELKPTAKFVPGKKVWEVPCDIALPCATQNELSLDDAKELIKNG
                     CIAVGEGANMPSTIDATNAFLQSKVLFAPAKAANAGGVATSALEMSQNSARMIWTFDE
                     VDEKLKDIMESIYGHMANAAKEYSTPDDFVAGANIAGFLKVADAMMAQGIV"
     gene            complement(1853..2848)
                     /gene="spoIID"
                     /locus_tag="ACZPE2_00360"
     CDS             complement(1853..2848)
                     /gene="spoIID"
                     /locus_tag="ACZPE2_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HIR1318329.1"
                     /translation="MKTYGILCFVLALFMILTPLLSLDYSSFSLSNVKSTFQKENDSK
                     DEEKLLDEKSDSVKIKSAASGNVATASTKEYIIGCVAAEMPATYSTEALKAQAVCAYT
                     NLVRLKKNPDSSLSGADISDDPGRHQGYYDIETQKEKWGDKYDEYRAKTEEAVTDVLG
                     KTIVFNGEPIVAAYCAISPGRTESAENIWGGKIEYLVSVTSPGDKLSPDCIKEISLSA
                     DEVREYTKNDPEIVLGDDQSGWITDIVYTDSGSGVVKSLKVGGKSFTGNSFRALFSLR
                     SPAFTLEQKDGAFFFKTYGYGHSVGMSQYGADYMAKQGSTYEEILAHYYPNTEII"
     gene            2969..4378
                     /locus_tag="ACZPE2_00365"
     CDS             2969..4378
                     /locus_tag="ACZPE2_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1318330.1"
                     /translation="MSYNHSPKLDDSLSSLKEELIRNNQIKLDDYHRYDVKRGLRNSD
                     GTGVMAGLSRICSVEGYYIDDGERVPKEGKLIYRGINMTDIVRNCQKENRFGYEEVVW
                     LLLLGDLPTQGQLDKFRAVLAEARELPDDFIEDMIMKAPSPNIMNKVARSVLALYSYD
                     NNPDDLSIENVLRQSIKLIAQIPTIMSYAYQVKRRAYDHKSMYIHQNDKALSTAESIL
                     HTIRSDRKFTDEEAKILDLCMIIHADHGGGNNSTFTTRCITSTGTDTYSAIAAGIGSL
                     KGPKHGGANIQVHNMIADLKKTVSNPTDEGQVADYLTKVIHKEAGDRTGLIYGMGHAV
                     YTLSDPRAVLLKEFASQKAEKYGFADDYKIISLVEELTPELFYKYKGEKKKMCANVDL
                     YSGLIYDMLRIPPELFTPMFMAARISGWCAHRLEELCSGSRIMRPAYKSVALPRAFVP
                     IAERNIDHIVPDYVPSEER"
     gene            4399..>5218
                     /locus_tag="ACZPE2_00370"
     CDS             4399..>5218
                     /locus_tag="ACZPE2_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318331.1"
                     /translation="MKKTGFEKSFNFKQILITLAAGAAVLLPVRIYQLFAIIDTGSTG
                     FYKKINFSVYFMYVAVVVFTALLIVFSRLSDDTRASRPAMCKNKLLGAAAVVMAVGTA
                     YDSADSMLSFSASCSDFSSNLDVSFMRYFFSNGMFASLFESIFGIIACVYFIVFALSY
                     FNDKADFTSYKVLALAPLFWAMCKMITRFMTKISFINVSELMLEMLELALVMLFFMSF
                     AKISSDVADKGEFKKLVSYGMPAAMLAAVIGVSRLVITLCGKRDRLPSELGFSVT"
BASE COUNT         1280 a         1229 c         1240 g         1469 t
ORIGIN      
        1 gtcaaaaagc ctcgcaaggc gacagccttg cttcgtttcc tgacttcggc ataaaaaaat
       61 ttttacggct acgaaactgc ggagcacttc aaaatgatgt attgatgtat ttttgataca
      121 aaaaaataga taacccctcg gatgagaccg gctcatctga ggggttaatt tgttattgtg
      181 caaaagatgt tattttgaag caaaactgcc tcataaacac tcagccaaga ctgtggctcc
      241 agttggtttt tgcgtataaa ttatactata ccctgtgcca tcatcgcgtc ggctaccttt
      301 aagaagcctg caatgtttgc gccggcaaca aagtcgtcgg gggtgctgta ttcctttgcg
      361 gcatttgcca tgtggccgta aatgctctcc ataatatcct tgagtttttc atcaacctca
      421 tcaaaggtcc aaatcatacg tgcgctgttc tggctcattt caagcgcaga ggttgcaacg
      481 ccgcctgcgt ttgccgcttt cgccggagca aagagtactt tgctctgtaa aaatgcgttt
      541 gtcgcgtcga tggtggacgg catatttgcg ccctcgccta ctgcgatgca gccgttttta
      601 ataagctctt ttgcgtcgtc aagtgacagc tcgttctgag tggcgcacgg cagagctatg
      661 tcacagggaa cctcccatac tttcttgccg ggaacgaatt ttgcggtggg ctttaattcg
      721 gtgtacctta ctattctcgc tctttcctgc tccttgacct gctttaagag cgcgatgtct
      781 atgccgtcct tatcgtagac atatccgctt gagtcgcttg cggtaactac ctttgcgcca
      841 aacgtttgag ctttttctat ggcgtaaatt gcaacgttgc ccgagcccga aacggcaacc
      901 gttttgccct cgatggaatt gccgtgattc tttaacatct cctcaacgag atagataagg
      961 ccgtaaccgg tagcctctgt tcttgcgagt gagccgccgt aggagagacc cttaccggtg
     1021 agcacgccct cgtggcggcc ggtaattttt ttgtactgac cgaagaggta gcctatctct
     1081 ctgccgccta cgcctatatc gcctgcgggc acgtcggagt tggggcctat aaccttgtaa
     1141 agctctgtca taaagcactg gcaaaatctc attatttcgt agtcgctctt gcccttgggg
     1201 tcaaaatccg aaccgccctt gccgccgcct atgggaagac cggtaagcga gtttttgaaa
     1261 atctgctcaa agccgaggaa tttaattatg ctcatattga ccgacgggtg gaagcgaagg
     1321 ccgcccttgt aaggccctat cgcgctgtta aactgaacac ggtagccgcg gtttacgtga
     1381 accgtgccgt tgtcgtcaac ccacggaact ctgaatacgg ttgccttttc cggtgccaca
     1441 agcctctcaa gaagagccgc cttctgatac ttcggatttc tctctacggc aggctctatg
     1501 gatttaagaa cctcggtgac agcttggcaa aattcgggtt gatagtcgta ccttgtcgtt
     1561 gtgcggttaa gcacttcgtc aatatagctc atttttagtc tccttttcgg caccgagcga
     1621 ataaagcccc gcaccgtttt tattacggta tatattttat attatgtgct ttatatttgc
     1681 aagaaacacg gcgtaaaatt tcattttgcc tccggcgata cttttgtgta ttataacgat
     1741 aaagtgaatt tgtattcgct gagacacgtg aaaacaaaca aaaatgcgta aaaagacatt
     1801 taaaaaaata attaccgtga caaaaaagat gaataaccgc acttataaac tttcaaataa
     1861 tttcggtgtt cggatagtag tgcgcgagaa tttcctcgta agtgctgccc tgttttgcca
     1921 tataatccgc cccgtactgc gacataccga cgctgtgacc gtagccgtat gtcttaaaga
     1981 aaaacgcacc atccttttgc tcgagcgtaa acgccggact tctcagcgaa aaaagtgctc
     2041 tgaaggagtt tccggtaaag ctcttgccgc cgaccttcag gcttttcacc acgcccgagc
     2101 cgctgtccgt gtaaactata tcggttatcc agccgctttg gtcgtctccc aagactattt
     2161 cggggtcgtt ttttgtgtat tctctgacct cgtccgcgga gagagatatt tcctttatgc
     2221 agtccggcga gagcttgtcg ccgggacttg tgaccgagac gagatattcg atttttccgc
     2281 cccatatatt ttcggcactc tcggttctgc cgggtgaaat tgcacagtat gccgccacaa
     2341 tcggctcgcc gttaaacact attgttttgc cgagaacgtc ggttaccgcc tcctccgttt
     2401 tggctcggta ttcgtcgtat ttatcgcccc atttttcctt ttgtgtttcg atgtcgtaat
     2461 agccctggtg acgtccgggg tcgtccgata tgtcggcacc cgaaagcgag gaatcgggat
     2521 ttttcttaag ccttacaagg tttgtatagg cgcataccgc ctgtgctttt aaggcctccg
     2581 tgctgtatgt tgcgggcatt tcggcggcga cgcagcctat tatgtattcc tttgttgagg
     2641 cggtagccac gttgccgctt gcggctgatt ttatttttac gctgtcggat ttttcgtcaa
     2701 gcagcttttc ctcgtccttg ctgtcgttct ccttttgaaa ggtgcttttg acgttggaga
     2761 gagaaaagga ggagtagtcg agcgataaca gcggcgtaag tatcataaaa agtgccaaaa
     2821 caaagcacaa aataccgtat gttttcataa ttgacctccc taattttatt gacttattgt
     2881 gatttttaag ataaaatatt atatctataa ttatatgctt aaaatgagtt aatatgtccg
     2941 cttaaatgta agtcaggagg ttttaaattt gtcttacaac cattctccta aacttgacga
     3001 ttcgctctcg tccctgaagg aagagcttat acgcaataac cagataaagc ttgatgatta
     3061 tcatcgttac gacgtaaagc gcggcttgcg taattccgac ggtaccggcg ttatggcagg
     3121 gctttcgaga atatgctctg ttgagggtta ttacatagac gacggtgaaa gagtgccgaa
     3181 ggagggcaag cttatttacc gcggtataaa tatgaccgat atagtgagaa actgccaaaa
     3241 ggaaaaccgc ttcggctacg aagaggtggt ttggctgctt ctcctcggtg atttgccgac
     3301 gcagggacag cttgataaat tcagagcggt tctcgccgag gcaagagagc tccccgacga
     3361 tttcatagag gatatgataa tgaaggctcc gtcgccgaat attatgaata aggtagcaag
     3421 gagcgttctc gcactgtatt catatgacaa caatcccgac gatctttcaa tagaaaatgt
     3481 gctgcgtcag tccattaagc tcatagcgca gatacccacc attatgtcat atgcgtatca
     3541 ggtaaagcgc agagcatacg accacaagag tatgtatatc caccagaacg ataaagcgct
     3601 ctccacggcg gagtccatac tccatacaat aaggagcgac cgcaagttta ccgatgaaga
     3661 ggccaaaata cttgaccttt gtatgattat tcacgccgat cacggcggcg gaaacaactc
     3721 cacgtttaca acacgctgca taacctcaac gggaacggat acctattccg caatcgccgc
     3781 aggtataggc tcgctcaagg ggccgaagca cggcggcgcg aatattcagg ttcacaatat
     3841 gatagccgac ctcaaaaaaa ccgtcagcaa cccaacagac gagggccagg tggcggacta
     3901 tctcaccaag gtcattcata aagaggcagg cgacagaaca ggccttatct acggtatggg
     3961 tcacgcagta tatactcttt ccgacccgcg cgctgtactc ttaaaggaat ttgcctcgca
     4021 aaaggcggaa aaatacggat ttgccgatga ctacaagata atttctcttg tcgaagagct
     4081 cactcccgag cttttctata agtataaggg cgaaaagaaa aaaatgtgcg caaacgtgga
     4141 tctttattcg gggcttatat acgatatgct cagaattccg ccggagcttt tcactccgat
     4201 gtttatggcg gcaagaattt ccggctggtg cgctcaccgt cttgaggagc tgtgctccgg
     4261 cagccgcata atgcgacccg catacaagag cgtcgctctt ccgagagcgt ttgttccgat
     4321 tgccgaacgc aatattgacc acatagtgcc ggattacgtt ccgtccgagg aaagataaaa
     4381 acggaaggac tcttataaat gaaaaaaacg ggcttcgaaa aaagctttaa ttttaaacag
     4441 attctcataa cgcttgccgc aggagcggcc gtgctgttgc cggtgcgtat atatcagctg
     4501 tttgcgataa tagacactgg cagtacggga ttttacaaaa aaatcaattt cagcgtttac
     4561 tttatgtatg tcgcagttgt tgtgtttacg gcactgctta tcgtgttttc gaggctttcc
     4621 gatgatacga gagcttcaag acccgctatg tgcaaaaaca agcttctcgg agcggcggcc
     4681 gttgttatgg ctgtcggtac ggcatatgat tctgcggatt cgatgctgtc gttctccgcg
     4741 tcctgcagcg atttttcgag taacctcgac gtgtcgttta tgagatattt tttctcaaac
     4801 gggatgtttg cgtcgctttt tgagagcata ttcggaataa tcgcctgtgt ttattttata
     4861 gtttttgcac tttcgtattt taatgacaag gccgatttta catcttataa ggtgctcgct
     4921 ctggctccgc ttttctgggc tatgtgcaaa atgataacaa ggtttatgac caagatcagc
     4981 tttataaacg tttcggagct tatgctcgaa atgcttgagc ttgcgcttgt gatgctgttc
     5041 tttatgtcgt ttgcgaagat ttcctccgac gttgccgaca agggcgagtt taaaaagctt
     5101 gtctcatacg gtatgccggc tgcgatgctg gcggcggtga taggcgtttc ccgtcttgta
     5161 attacgctgt gcggaaaaag agaccgcttg ccgtccgaac tcggattttc cgtaaccg
//
LOCUS       DBMDVO010000013         5218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6448_length_5218_cov_1.128005, whole genome shotgun sequence.
ACCESSION   DBMDVO010000013 DBMDVO010000000
VERSION     DBMDVO010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_5218_cov_1.128005"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            51..1061
                     /locus_tag="ACZPE2_00375"
     CDS             51..1061
                     /locus_tag="ACZPE2_00375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1318332.1"
                     /translation="MLNELCNLNGISGREHAVRDYIISNLPSDAEYRIDPLGSLIVYK
                     KGKAEPKNKVMLDAHMDEVGFIITYITDDGYLKFTTVGGIDERVIFGRAVKVGKELIP
                     GVIGGKAIHQTTSEERGKLPSVEDMYIDIGASSKKEALSHVSLGDAVYFDSCYREFGD
                     GFIKAKAIDDRVGCEILLRLINSDLPYSATFCFSVQEEIGTRGAAAAAYSVAPDFAIV
                     VEGTTAADISGVPDEKKVCRLGGGAVVSFMDRGTVYDSELYKKAFEIAEKNGIGCQTK
                     TVVAGGNNASAIHKAAGGIKTLAVSVPCRYIHSGSSVAKKEDIESTFELVKALFAELS
                     QC"
     gene            1055..1780
                     /locus_tag="ACZPE2_00380"
     CDS             1055..1780
                     /locus_tag="ACZPE2_00380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1318333.1"
                     /translation="MLKSIDGPTEAAIVTDGEFKDCLAVKYILFAAFRLSLGEAWLQT
                     DCGGRVTAVLLNKNDGTIICPSSSVDFRELSEFASFFGENIYFCSGDDCADAKAVLME
                     LSELPQKETCAQPLCDITADEYKVLTGKTPDSSDRAYLEWLSRTGRGVFGGSTRVMCI
                     RQNGKTASLAVGDIIGKDAYIRDVATSEKYRGRGFAADCVISLSRELKKSADCIFLMC
                     KPENAKLYEKCGFRKKEYIIRKT"
     gene            1782..3059
                     /locus_tag="ACZPE2_00385"
     CDS             1782..3059
                     /locus_tag="ACZPE2_00385"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1318334.1"
                     /translation="MKDFFEFTKRTEEASKKALTLAEKKFSEIDEITEYNQRKVLAAF
                     INNRVDETDFNSTTGYGYNDKGREKLDKLTAEIFGAEDGLIRATTLTCGTHTIATALY
                     GVLRPGDIMLSVTGVPYDTIHSVIGIDKSSKGHGSLADFGVKFEYIELTENDEIDYDA
                     IEKRVGCGDIKMVYIQRSRGYSLRHSISVDEIEKIATLTHRVSPDTVVMTDNCYGEFT
                     EKKEPCDVGADLAAGSLIKNPGGAIAPCGGYIVGRHDLVEDCAYRMTTPGLGREVGAT
                     LGTTRSLYMGLFSSPHVTGEALKTAVFAAALFDLLGFRVTPRPDETRHDIIEAICLEN
                     ADRLIAFCQGMQKGAPVDSYVSPVPWDMPGYDSQVIMSAGAFTSGSSIELSSDAPLRE
                     PYAVWMQGGINFHSGKLGVMLAADEIIKRGLLE"
     gene            3092..4600
                     /locus_tag="ACZPE2_00390"
     CDS             3092..4600
                     /locus_tag="ACZPE2_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR1318335.1"
                     /translation="MELRHLKSGTDVRGTAVNPDDPKKIDLTDEAVKRISAAFVVFLA
                     EKLGKKEEELVISVGHDSRISAERIKSDVIDALSFFGLEIKDCALASTPAMFMTTKML
                     GCDGAVQITASHHPWDRNGLKFFTPDGGLSGDEIKSILEYAEEHESGSCPERLNLQEV
                     DFMSVYCEHLCNMIRKGVKSDDYERPLKGLKIVVDAGNGVGGFYAEKVLSPLGADTTG
                     SRYLEPDGMFPNHIPNPENETAMNSVREATLGAKADLGVIFDTDVDRGGCVSSDGREI
                     NRNALVALAAVIALESNPGGTVVTDSVTSAGLTEFIEKTLGGHHLRFKRGYKNVIDEA
                     IRLEKNGINAPLAIETSGHAAFRENYYLDDGAYLITKIIIKAADMKKQGKKLYDLISE
                     LKYPAEEKEIRLKITEEDFRAVGDRLLEELADFAAKREDWQIAPDSHEGIKIIFPKTE
                     SFFILRQSVHDPVIPINFESAQNGGVKKAANSLYEFIKDFDGVDISPLKQIL"
     gene            4626..4856
                     /gene="acpP"
                     /locus_tag="ACZPE2_00395"
     CDS             4626..4856
                     /gene="acpP"
                     /locus_tag="ACZPE2_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1318336.1"
                     /translation="MFTVYDTICEILSEQLEIPLDEITAESKLRDDLSADSLDLIDLA
                     MTLEDEFRKEVPDEALEKMKTVGDVVAFIEEE"
     gene            4986..>5218
                     /locus_tag="ACZPE2_00400"
     CDS             4986..>5218
                     /locus_tag="ACZPE2_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1318337.1"
                     /translation="MTKVNFYDSINDSMLKSAVIIARHNGKWVFCKHKERNTWEAPGG
                     HREDGEDILETAKRELYEETGAITFDITPICIYS"
BASE COUNT         1472 a         1145 c         1415 g         1186 t
ORIGIN      
        1 tgagctttgc gcaaggctca ttgccgcata cattctcgga ggtgatttaa atgcttaacg
       61 agctttgtaa tttaaacggc atttccggca gggagcacgc cgtaagagat tatataattt
      121 cgaatttgcc gtcggacgcg gaatatcgca tagatccgct cggcagtctc atagtttata
      181 aaaagggcaa agccgagccg aaaaataaag ttatgctcga cgcgcatatg gacgaggtcg
      241 gcttcataat aacttatata acggatgacg ggtacctgaa gttcaccacg gtcggcggaa
      301 ttgacgagcg cgttattttc ggcagagcgg taaaggtggg caaggagctt atccccggcg
      361 ttataggcgg caaggctata catcagacga cctccgagga gagaggtaaa ttgccgtccg
      421 ttgaggatat gtatatcgat atcggcgcga gttcaaaaaa agaggccttg tctcacgttt
      481 cactcggcga cgcagtgtat tttgattcgt gctaccgcga attcggcgac ggctttatca
      541 aggcgaaggc cattgacgac cgcgtcggct gtgaaatttt attaagactt ataaattccg
      601 atttgccgta ttcggcgacc ttctgtttct cggttcaaga ggaaataggc actcgcggag
      661 cggctgctgc ggcttattcg gttgctcccg atttcgcaat agtcgttgag ggcactacgg
      721 cggcggacat ttcgggcgtt cccgacgaaa aaaaagtctg ccgtttgggc ggcggtgccg
      781 ttgtcagctt tatggacagg ggaaccgtct atgacagcga gctctataaa aaggcgtttg
      841 aaattgccga aaagaacggc atagggtgtc agaccaaaac cgttgtggca ggcggcaaca
      901 acgcttccgc aatacacaag gcggcaggcg gcataaaaac tcttgccgta tctgttccgt
      961 gcagatatat tcactcgggc agctcggttg cgaaaaaaga ggatatagag agcacatttg
     1021 agcttgtaaa agcacttttt gcggagctgt cgcagtgctg aaatcgattg acggaccgac
     1081 agaggcagcc attgttacgg acggtgaatt taaagactgt cttgcggtaa agtatatttt
     1141 atttgccgct tttcggctgt cactcggaga ggcgtggctt caaacggact gcggcggcag
     1201 agtgacggct gtgcttttaa ataaaaacga cggaacgata atttgcccgt caagctccgt
     1261 cgattttcgg gagctttcgg agttcgcctc ttttttcggc gaaaatatat atttttgctc
     1321 cggggacgat tgcgcggacg caaaagccgt tcttatggag ctctccgaat taccgcaaaa
     1381 agaaacttgc gcacagccgc tttgcgacat aaccgcggac gaatacaagg tgctgacagg
     1441 taaaacgccc gattcaagcg acagggcgta tctcgaatgg ctttcgagga cgggcagagg
     1501 cgtgttcggc ggaagcacaa gggttatgtg cataaggcaa aacggcaaaa cggcttctct
     1561 tgccgtaggc gatataatcg gaaaagacgc gtatatacgc gacgttgcga caagcgaaaa
     1621 atatcgaggc agaggctttg ccgcggactg tgttatttct ctttcgagag agcttaaaaa
     1681 gagtgccgat tgcatatttt taatgtgtaa gcccgaaaat gcaaagctgt atgaaaaatg
     1741 cggattccgc aaaaaagaat acataataag aaagacttga aatgaaagat ttttttgaat
     1801 ttacaaaaag aaccgaggag gcgtcaaaaa aggcgctgac tctcgccgaa aagaagtttt
     1861 cggaaataga cgaaataacc gaatacaacc agcgcaaggt gctcgcggcg tttataaata
     1921 atcgcgttga cgagacggat ttcaactcca ccaccggcta cggctacaat gataagggca
     1981 gagaaaagct cgacaagctc accgccgaga ttttcggagc ggaggacggc ttaataagag
     2041 ccaccacctt aacctgcgga acccacacaa tagcgaccgc gctttacggc gtactaaggc
     2101 ccggcgatat aatgctcagc gtaacgggag tcccctacga caccatacat tccgttatcg
     2161 gtattgacaa aagcagtaag ggtcacggct cactcgccga cttcggcgtg aaattcgaat
     2221 atatcgagct taccgaaaat gacgagatag attatgacgc aatagaaaaa agagtcggct
     2281 gcggagacat taagatggtt tatatccagc gctcgcgcgg gtattcgctc cgccattcga
     2341 tttcggtaga cgaaatagag aaaatcgcga ctcttacaca ccgtgtaagc cccgatacgg
     2401 ttgttatgac agacaactgc tacggcgaat ttacggagaa aaaagagccg tgcgatgtcg
     2461 gagcggattt ggcggcaggc tcgcttatca aaaatccggg cggcgcaata gctccctgcg
     2521 gcggctatat agtaggcagg catgaccttg ttgaggattg cgcatacaga atgaccactc
     2581 ccggactcgg cagagaggtc ggcgcaacgc tcggaacaac gcgcagcctt tatatgggcc
     2641 ttttttcttc gccgcacgta accggcgagg cactcaagac cgcggtattt gcggcggcgc
     2701 ttttcgattt gctcggcttt agggtgactc cgcgtcccga tgagacgaga cacgatatta
     2761 tagaggcgat ttgccttgag aatgccgaca gactgatagc cttttgtcag ggaatgcaga
     2821 agggcgcgcc ggtggattca tacgtttcac ccgtgccgtg ggatatgccg ggctacgaca
     2881 gccaggttat aatgtccgca ggggcgttca cgtccggctc gtccatagag ctgtcatcgg
     2941 acgcaccgct tcgagagccg tatgcggttt ggatgcaggg cggaatcaat tttcacagcg
     3001 gaaagctcgg cgttatgctg gcggcagatg agataattaa aagaggtctt ttggaataat
     3061 ttacggaata acttacggaa aggttgattt tatggaactc agacatctta aaagcggaac
     3121 cgacgtcaga ggcacggcgg taaatcccga cgatccgaaa aaaatcgatt tgaccgatga
     3181 ggcggtaaag agaatttccg cagcgtttgt cgtatttctc gccgaaaagc tcggtaagaa
     3241 agaggaagag cttgtgattt cggtcgggca tgattcgaga atttcggcgg agcggataaa
     3301 atccgacgta atcgatgcgc tgtctttctt cggccttgaa atcaaggact gtgcgctcgc
     3361 atcaaccccg gctatgttta tgacaactaa aatgctcggc tgcgacggag cagttcagat
     3421 aaccgcgagt caccatccgt gggacagaaa cgggcttaaa ttctttactc ccgacggagg
     3481 actttccggt gatgaaataa agagtatact cgaatatgcc gaggagcacg aaagcggcag
     3541 ctgccccgaa aggttaaatt tgcaggaggt cgattttatg tcggtctact gcgagcacct
     3601 ttgcaatatg ataagaaagg gtgttaagtc agacgattac gaaagaccgt taaagggact
     3661 gaaaattgtt gttgacgcag gtaacggagt aggcggattt tacgccgaaa aggtactctc
     3721 ccccctcgga gcggacacaa ccggcagccg ctatttggag ccggacggta tgttcccgaa
     3781 tcatatcccg aatcccgaaa atgagacggc gatgaactcc gtgcgagagg caactctcgg
     3841 cgcgaaagcc gacctcggcg taatatttga cacggacgtt gaccgcggcg gctgcgtaag
     3901 ctccgacggc agagagataa acagaaacgc tcttgtcgcc ctggcggcgg tcattgcgct
     3961 cgaaagcaat ccgggcggca ccgttgtcac cgattccgta acgtctgcgg ggcttaccga
     4021 gtttatagaa aaaacgttag gcggacatca tctgcgcttt aagcgcggct ataagaacgt
     4081 cattgacgag gcgataagac ttgagaaaaa cggtataaac gctccgcttg ccatagagac
     4141 ttcgggtcac gcggcgttca gggagaatta ttatcttgac gacggcgcat atctcataac
     4201 gaaaataatt ataaaggccg ccgatatgaa aaagcagggt aaaaagcttt acgacctcat
     4261 atcagagctt aaatatccgg cggaagaaaa ggaaatccgc cttaaaataa ccgaggagga
     4321 cttcagagcc gtcggcgaca ggcttttaga ggagctcgca gattttgccg caaagcgcga
     4381 ggactggcaa attgcacccg acagccacga gggaatcaaa ataattttcc caaagaccga
     4441 gagcttcttt attttaaggc agagcgtcca cgacccggta ataccgataa atttcgagag
     4501 tgcccaaaac ggcggagtga aaaaagccgc aaactcgctc tatgaattta taaaagattt
     4561 cgacggggtg gacatttctc cgctcaaaca gattttataa ccgcgttaaa tatgtacgga
     4621 ggtgcttgtt tacggtatac gatactatat gtgaaatttt gagcgagcag cttgaaatac
     4681 cgctcgacga aataaccgcc gagtcaaagc tgcgcgatga tttatcggca gacagccttg
     4741 accttatcga cctcgctatg actttggagg acgaattcag aaaagaggtg ccggacgagg
     4801 cgcttgaaaa gatgaaaacg gtgggagacg tagtagcatt catagaggaa gagtaacgga
     4861 ctaaaatgcc gaaagcctgt atttatgcgg gcttccggca ttttttatca aacggctctg
     4921 gtttggctct gtttgtgaga gcaataatgg caagaaaata gatgtaatct aataaggagc
     4981 attatatgac aaaagtgaat ttttatgata gtattaatga ttcaatgctg aagtctgcag
     5041 ttattattgc cagacataat ggtaaatggg tattttgcaa gcacaaggaa agaaatacat
     5101 gggaggcacc ggggggacat agagaagatg gtgaggatat tcttgaaaca gccaaacgag
     5161 aactatatga agaaacagga gcaataactt ttgatattac tccaatttgt atatattc
//
LOCUS       DBMDVO010000014         5140 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_6607_length_5140_cov_1.132963, whole genome shotgun sequence.
ACCESSION   DBMDVO010000014 DBMDVO010000000
VERSION     DBMDVO010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_5140_cov_1.132963"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ACZPE2_00405"
     CDS             complement(<1..449)
                     /locus_tag="ACZPE2_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR1318338.1"
                     /translation="MKKFFAVMLSLMLTVLTFASCQSTDKTNSTTNSDPGTNNTGKKY
                     KVAIVQLADNGAFTEMRESFIQEMRTLGYDEAQMIFDIKNAQGDQSTLNTISQGLKNG
                     DYDLIVPIVTPATMSVVNQGLKTPVVFISVTNPVGSGIMTSMEKPDK"
     gene            846..1145
                     /locus_tag="ACZPE2_00410"
     CDS             846..1145
                     /locus_tag="ACZPE2_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR1318339.1"
                     /translation="MVRHIIFWTFSDNVNDSNRKEVLKMMSDSVKNLVGKIPGLIKAE
                     IGENFADSDCDFVFYAEFERMENIPAFQVHPLHVAHKERSAPYVKTRFVADFVSE"
     gene            complement(1154..2131)
                     /locus_tag="ACZPE2_00415"
     CDS             complement(1154..2131)
                     /locus_tag="ACZPE2_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1318340.1"
                     /translation="MKTFFTVLFITAAVIFLVCIIFRGETPDKPKKNIKSDNPAILPY
                     GKTGVLAHRSGAGLAPENTLLAFQNMIKNRDKFELEGFEFDLHLTKDGQIIVLHDDTL
                     DRTSDSEEYFGKSGVLPCDLSFDELRKLNMGEKFVLPDGSIPYEGLRGDKIPDSLRII
                     RLSDVFDFLAPYGDYTFTVDIKNDGDIGYRTADELHRLLVKYNMLSRTAVASFHKSNI
                     RYLDEKYPDITRSATIHEVVRFYFQSFFHIPYKQGHFKFKYLHIPYKDYFFNLGTTRL
                     INYAHSYGIAVQYWTINDPKIMKRLEKNGADAVFTDLPDLAYETLNDIK"
     gene            complement(2297..5023)
                     /gene="secA"
                     /locus_tag="ACZPE2_00420"
     CDS             complement(2297..5023)
                     /gene="secA"
                     /locus_tag="ACZPE2_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1318341.1"
                     /translation="MSLLTKIFGDYSSKEVKRVMPIQKKVLSYEDEYSRLSDEALKAK
                     TPEFKERLKNGETLDDILPEAFAVCREASSRVLNMKHFPVQIIGGIILHQGRIAEMKT
                     GEGKTLVATLPAYLNALSGKGVHIVTVNDYLARRDSEWMGKLYRFLGLSVGLIVHELN
                     NDERRAAYSADITYGTNNEMGFDYLRDNMIAYAEQRVQRGHSFAIVDEVDSILIDEAR
                     TPLIISGVGDKSTDLYKIADSFAKTLKKVTVKELDSKQNAEEELSEDGDFVVDEKAKT
                     ATLTKSGIAKAEAYFNLENLMDSENITVLHHIEQAIKAIGVMKRDVDYVVKDGEVLIV
                     DEFTGRIMLGRRYSEGLHQAIEAKEGVKVERESKTLATITFQNYFRLYDKLSGMTGTA
                     MTESTEFQEIYSLDVISIPTNKPMIRIDDPDVLYKTEQAKFNAIIDRIIECHENGQPV
                     LVGTISIEKSEALSKALKKRGIKHEVLNAKYHEKEAEIIAQAGKFGAVTIATNMAGRG
                     TDIILGGNPEYMAKAEMRKKGYSDELIAEATGYAETSDEEILEARKTFRELENKHKEE
                     LKDEAAKVRNAGGLYIIGTERHESRRIDNQLRGRSGRQGDPGHSQFFLSAEDDLLRLF
                     AGDRFYNILNNFKAVGDMPIEAGILSKSIESAQKKVEANNFATRRNVLEFDDVMNEQR
                     KKIYSERNQVLDGADLHETIQKMINDYFDSMVQFYCPSSLPENEWNLAGMKTKLGWII
                     GSDSLDELKTPDDYLKYFLSKAEKLYNDRKQKYGAEIMSLLERKILLQNVDRHWMDHL
                     DAMEELKRGIYLRSYGQQNPIVAFRMESYDMFEEMTDAIREGTVTGILTVVIRTEADT
                     KREQQGKITSTSGATDGSEKKQPVRKAKKPGRNDPCPCGSGKKYKYCCGKDD"
BASE COUNT         1176 a         1190 c         1210 g         1564 t
ORIGIN      
        1 tttttatcgg gcttttccat gcttgtcata atgccggagc cgacgggatt tgtaacggaa
       61 ataaatacga cgggagtttt aaggccttga tttacgactg acattgtggc gggcgtaact
      121 atcggaacta taaggtcgta gtcgccgttt ttaagtccct ggcttatggt gttgagcgtg
      181 gactgatcgc cctgtgcatt tttgatgtca aatatcatct gcgcttcatc gtagccgagt
      241 gttctcatct cctgaataaa tgactcgcgc atctctgtga aggcaccgtt gtctgcgagc
      301 tgaactattg cgaccttgta cttcttaccg gtgttgtttg tgccggggtc ggagttcgtg
      361 gtgctgtttg ttttgtctgt gctttggcag gatgcaaatg tgagcaccgt gagcataagt
      421 gacagcataa ctgcaaaaaa ctttttcatt ttaaaatcct cctgaaatct tctgtataat
      481 aaaaaagctg cccttgtaaa aatacaagga caggaaatta cctgcggtac caccttgatt
      541 agcgtaaaac gctcactctg acggaatact gacatattcc tctttctgta acgtgaaagc
      601 tccgtcgcaa cttttcattg cgccctcagc ggaccatttg tctgcaccgt aacaaccgga
      661 cttacaccgc ctccggctct ctgaatgttc gttttgcagt tttacttccg tttcatcggt
      721 ttaggtcatt aaaacacact ttaaattttt tgtcaagggt ttatttttga tttttttatg
      781 atacaataac atcggtatag ttgctttgcg cggtaccgat attttcccaa ggggcgtgtt
      841 acacaatggt aaggcatatt attttttgga cattttccga caatgtcaat gattcaaacc
      901 gtaaagaggt actgaaaatg atgtctgact cggttaagaa tctcgtcgga aagattccgg
      961 gactcataaa agcggaaatc ggtgaaaact tcgccgacag cgactgcgat tttgtatttt
     1021 atgcggaatt tgagcgtatg gagaatattc cggcatttca ggtgcatccg ctgcatgttg
     1081 cccataagga gcgttcggcg ccgtatgtaa aaacacgctt tgtcgctgac ttcgtttcgg
     1141 agtaatcaaa acattattta atatcgttga gcgtctcgta agctaagtcc ggcaggtctg
     1201 taaagactgc gtccgcaccg ttcttttcga ggcgcttcat tattttcggg tcgtttatgg
     1261 tccagtactg aacggctata ccgtagctgt gggcataatt tataagcctt gtagtaccga
     1321 ggttgaagaa gtaatcctta tacggaatgt gcagatattt aaacttgaaa tgaccctgct
     1381 tatacggaat gtgaaagaaa ctctgaaaat agaatctgac cacctcatgt attgtggccg
     1441 aacgtgttat gtcgggatat ttttcgtcaa gataccgaat attgctcttg tgaaatgagg
     1501 ctacggccgt gcgtgagagc atattatact ttaccaaaag gcggtgaagc tcatctgcgg
     1561 tgcggtaacc gatgtcaccg tcgtttttaa tatcaacggt aaaggtgtaa tccccgtacg
     1621 gagcaagaaa atcaaatacg tcgctgagcc ttataatgcg gagcgagtcc ggaattttgt
     1681 cgcctcgcag accctcgtaa ggtatagaac cgtcgggaag aacaaacttt tcgcccatat
     1741 taagctttcg cagctcatca aagctcaggt cgcacggcaa cacgccggac ttgccgaaat
     1801 attcctccga atcgcttgtg cggtcaagcg tgtcgtcatg caggactatt atttggccgt
     1861 cctttgtgag gtgcaggtca aattcaaagc cttcaagttc gaatttatcc ctgttcttga
     1921 tcatattctg aaaggcgagc agagtatttt ccggagcgag ccctgcccct gagcgatgag
     1981 ccaaaactcc cgtcttgcca tatggcagta tagccggatt gtcgctctta atgttcttct
     2041 tgggcttgtc gggcgtttcc cctctgaata tgatgcatac taaaaatata actgctgccg
     2101 taataaacag caccgtaaaa aatgttttca ttttgccctc ccgcttaccg cgcatataat
     2161 gtgcataatt aacaatatat attgtattat aagtcgattt atataataag tcaataaaca
     2221 aagacgaaaa gaaagacgga cataaaacac aagctttata tccgtctttt aaatttattt
     2281 atgagtcgcc gttatatcag tcgtccttgc cgcagcagta tttgtacttt ttaccgcttc
     2341 cgcatgggca cgggtcgttt ctgccgggct ttttggcttt tctgaccggc tgcttctttt
     2401 cactgccgtc ggtagctccg cttgtggagg taatcttgcc ctgctgctcg cgctttgtgt
     2461 ccgcttcggt gcggattaca accgtaagaa tgccggtgac cgtaccctcg cgaatggcat
     2521 cggtcatttc ctcaaacata tcgtagctct ccatacggaa tgctactatc gggttctgct
     2581 gaccgtagga acgaaggtaa ataccgcgct ttaattcctc cattgcgtcg aggtgatcca
     2641 tccagtggcg gtctacgttt tgaagaagta tttttctctc gagcaggctc ataatctccg
     2701 ctccgtactt ctgcttgcgg tcgttgtaaa gcttttcggc ttttgagagg aagtatttaa
     2761 gataatcgtc gggggtcttg agctcgtcga gcgaatcgga gcctataatc cagccgagct
     2821 ttgtcttcat accggcgagg ttccactcgt tttcgggaag tgacgacggg cagtagaact
     2881 gaaccatact gtcaaagtag tcgtttatca tcttctgaat cgtttcgtga agatcagctc
     2941 cgtcgagcac ctgattgcgt tcgctgtaaa tctttttacg ctgctcgttc ataacgtcgt
     3001 cgaattcgag gacgttacgg cgcgttgcaa agttgttggc ctctaccttt ttctgagcac
     3061 tctcaattga ttttgaaaga atgccggcct ctatcggcat atcgccgact gccttgaagt
     3121 tgttaaggat gttatagaat ctgtcacctg caaagagcct taaaaggtcg tcctcggcag
     3181 agaggaagaa ctggctgtga ccggggtctc cctgacgtcc cgaacggccg cgcagctgat
     3241 tgtcgattcg tctcgactcg tggcgttccg tgcctatgat gtaaagaccg cctgcatttc
     3301 taacctttgc ggcttcgtcc ttgagctctt ccttgtgctt gttttcaagc tcacggaatg
     3361 tctttctcgc ttcgagaatc tcttcgtcgg aagtctctgc ataacctgtt gcctcggcaa
     3421 taagctcatc cgaatagcct tttttacgca tctcggcctt tgccatgtat tcggggtttc
     3481 ctccgagaat aatatcggta ccgcggcctg ccatattggt ggctatcgtc acggctccga
     3541 atttacctgc ctgagctatg atttcggctt ctttttcgtg gtacttggcg ttgagtacct
     3601 cgtgctttat accgcgtttt ttgagtgcct ttgacaaagc ctcgctcttt tcgatgctta
     3661 tcgtacccac gagcacgggc tgaccgtttt cgtgacattc tataatgcgg tcgattatag
     3721 cattgaattt agcctgttcc gttttgtaga gaacgtccgg gtcgtcgatt cttatcatcg
     3781 gcttgttagt cggaatcgaa atgacatcga gagagtagat ctcctggaac tcggtgctct
     3841 ctgtcatggc ggtaccggtc atacccgaaa gcttgtcgta aagtctgaag taattctgga
     3901 aggtgatggt ggcgagcgtc ttagactctc tctctacctt aacgccctcc tttgcctcga
     3961 tagcctggtg cagaccctcg ctgtaacgac ggccgagcat tatacgtccg gtgaattcgt
     4021 caactatgag aacttcgccg tcctttacaa cgtagtcaac gtcgcgtttc ataacgccta
     4081 tggcctttat tgcctgctct atgtggtgga gaacggttat gttctcgctg tccataaggt
     4141 tttcgaggtt aaaatatgcc tctgcctttg caataccgga ttttgtgagc gttgcggttt
     4201 ttgccttttc gtcaactacg aagtcgccgt cctcggaaag ctcctcctcg gcgttctgct
     4261 ttgagtcgag ctcctttacg gtaacctttt taagggtttt tgcgaagctg tcggcaatct
     4321 tataaaggtc tgtcgacttg tcgccgacgc ctgatatgat aaggggtgtt ctcgcttcat
     4381 cgataaggat cgagtcaacc tcgtccacga tggcaaacga gtggccgcgc tgaactctct
     4441 gctccgcata ggctatcata ttgtctcgaa gatagtcaaa gcccatctca ttgtttgtgc
     4501 cgtaggttat gtcggcggag tatgccgccc ttctctcgtc gttgttgagc tcgtggacta
     4561 taagtcctac ggaaagaccg aggaagcggt agagcttgcc catccactcc gagtcgcggc
     4621 gggcaagata atcgttgacg gttactatgt gaacgccttt tccggaaaga gcgttgaggt
     4681 aagcgggcaa tgttgcgacg agggttttgc cttcgcccgt tttcatctcg gcaatacgac
     4741 cctggtgaag tatgataccg ccgattatct gaaccgggaa gtgcttcata ttgagaactc
     4801 ttgatgaagc ctcgcggcat accgcaaatg cctcggggag aatatcgtcg agcgtttcgc
     4861 cgtttttaag tctttctttg aactccggag tcttcgcctt cagcgcctcg tcggaaaggc
     4921 gagaatattc gtcctcatag cttaatacct ttttttgaat gggcattacg cgttttactt
     4981 ctttcgacga ataatctccg aaaattttgg ttaacagtga catttatcgt gtttcctttc
     5041 gtaaaaagaa tggagtttgc cgtatacggg ttacattata ccatacatat ttaaaaaaat
     5101 cattaaaaat ttttataaaa ttgcaaatgc tttttaaata
//
LOCUS       DBMDVO010000015         4936 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7066_length_4936_cov_1.128852, whole genome shotgun sequence.
ACCESSION   DBMDVO010000015 DBMDVO010000000
VERSION     DBMDVO010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7066_length_4936_cov_1.128852"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            150..866
                     /locus_tag="ACZPE2_00425"
     CDS             150..866
                     /locus_tag="ACZPE2_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318342.1"
                     /translation="MTALYIILGIIAFICLVLLVKVRIDAEYIDGFKLSVSWLFLHFS
                     IYPKTQKEKKPPKPKKEKPKKEKQEEPAEEKPKKPNIFKTFYENQGMDGVIKLVKDSA
                     DAVGLMMRSVKKHFIIEELYLWIVVSSNSDAAQTALQYGEICEDVFPALGFICSNLKV
                     KKYDASIEPDFIGTFSSAQFKTAFSLRPFFLVGAGIRLVFRLLFKVVLKLLRQKPKKA
                     VVTEGDNKTETQNIQGGASK"
     gene            863..1315
                     /locus_tag="ACZPE2_00430"
     CDS             863..1315
                     /locus_tag="ACZPE2_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR1318343.1"
                     /translation="MKEQSAAGILETTIEKVKNLVNVSTIIGEPMNIDGGITIIPVSK
                     VTYGFASGGSDFPSKTNKEIFGGGGGAGVTITPVAFLVISDGEVTIKHITAFDNAAER
                     VVNLVPEMFDKVTSVVNKAKKEKEKAASNDAAEYVSAVPAEDVPSENK"
     gene            1384..2529
                     /locus_tag="ACZPE2_00435"
     CDS             1384..2529
                     /locus_tag="ACZPE2_00435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1318344.1"
                     /translation="MYKRLFCVFLSVAFTVLCAFPAYAENDELDLSARAAILMTSDTL
                     EVVYAKNEHERLPMASTTKIMSALLALEYKSPDEAVTVTKEAVSVEGTSMGLLPGDCV
                     SVKTLACGMLLSSGNDAANVAAYAVSGGLDEFTALMNQRAKELNMNDTSFETPSGLDS
                     ENHYSTAYDMALLGAEAIKNPEFLSICSKKSMSVSYGNPPYRRTVTNHNRLLTIDEDC
                     IGIKTGFTKKSGRCLVSAVRRGGVTLIAVTLNAPDDWNDHEKMYNYGFSVVKNRSLFC
                     DLSGVRLSVVGSDKSSVGVKPLYTPYLASLKNIEPEIKICIKNFEYAPVESGKNVGTV
                     YYLYDGRVLSEVPIVTSEGAEYISDSEKMQKKSLSQKIKKFFRKVLW"
     gene            2535..3299
                     /locus_tag="ACZPE2_00440"
     CDS             2535..3299
                     /locus_tag="ACZPE2_00440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1318345.1"
                     /translation="MTDVKIRLQKFMAESGVASRRKSEELIAAGKVKVNGKTACVGDK
                     VNPKTDKVVVAGKKLSAPKENVYIMLNKPRGFITTMSDEMDRKCVAELVSDIDARIYP
                     VGRLDRESEGLLLMTNDGEFANALTHPSMHVPKTYRVTIHPSLSKEQRAALENGVEID
                     GKMTLPSEVRVVSREEGRVVIELTIFEGRNRQIRKMCESLGLEVARLKRIKIGSVKLG
                     MLKTGDYRHLTPDEVHALMTSAEQSGNLRRGLNDKI"
     gene            3286..3657
                     /locus_tag="ACZPE2_00445"
     CDS             3286..3657
                     /locus_tag="ACZPE2_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318346.1"
                     /translation="MIKFECVTELSELQCLFPGEPFYDNVRYAAYKTEYGGNDFSLLF
                     SLNGNICILERIKSDKNEPSVTDGLLRSALDFAFRRGVDTAVCSLFEFSDELISLGFT
                     QKDGVFSGNTAELLQGSCCKH"
     gene            3788..4666
                     /locus_tag="ACZPE2_00450"
     CDS             3788..4666
                     /locus_tag="ACZPE2_00450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1318347.1"
                     /translation="MIKSMTGYGKSEQTIDSLNVTVEIKSVNHRYFEFSARVPREYGF
                     LEEKLKKYCNSLITRGKVECYVSVEDLEEREMEVNVNETLAAGYVKALKELSERFGLK
                     DDISAVTLSRYPDVITLHKASEDEERIWNAVKTVAETAVSKFIEMRETEGSKLRGDIL
                     SRADYIIECVEFIEGRSPETVREYNEKLKLRMKELLGDAAVDEQRLLNEAAIYADKIA
                     VDEETVRLRSHISQLREFMNSSEAIGRKLDFLVQEINREANTIGSKAQDVDIAKKVIA
                     IKAEVEKIREQVQNIE"
     gene            4678..4932
                     /locus_tag="ACZPE2_00455"
     CDS             4678..4932
                     /locus_tag="ACZPE2_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HIR1318348.1"
                     /translation="MKLINIDFGNAVNTDRILAVAGPESAPIKRIAQEAKEQKMLIDA
                     TQGRKTRAVIITDSNHVILTYLQAETIMARFNDDSDKEQA"
BASE COUNT         1507 a          994 c         1213 g         1222 t
ORIGIN      
        1 agaagaaaat cacgaagaaa gcaaaaaagt tgacaattcc gacataaatt ctcttgaata
       61 atgttgcgtt tttttatata atctattaca tagtctcgca aaatgtgcgg gataggagta
      121 aattgtgaag cgaaggaggt gcccgtaggt tgacagcgct gtacataata ctcggtataa
      181 tcgcatttat ttgtcttgta cttttagtaa aagtcaggat tgatgcggaa tatatcgacg
      241 gctttaagct cagcgtaagc tggctgtttt tgcatttcag catttacccg aaaacccaaa
      301 aagaaaagaa acctcctaag cccaaaaagg aaaaacccaa aaaagaaaag caggaagagc
      361 ccgcagagga aaaacccaaa aagcccaata tctttaaaac cttctatgaa aaccagggta
      421 tggacggcgt aataaagctt gttaaggatt cggcagacgc cgtcgggctt atgatgcgca
      481 gcgtcaaaaa gcactttata attgaggagc tgtacctttg gatagtcgtt tcaagtaaca
      541 gcgacgccgc tcaaaccgct cttcagtacg gtgagatatg tgaggatgtt ttcccggctc
      601 tcggatttat ctgctctaac ttgaaagtga aaaagtatga tgcgagcatt gaacccgatt
      661 tcataggcac attctcaagc gcgcaattta aaacggcatt ttcattacgc ccgtttttcc
      721 ttgtcggagc aggcataagg cttgtgttca gacttttatt taaggtcgta ctcaaattac
      781 tgcggcaaaa gcccaaaaag gcagttgtca cagagggaga taataaaaca gaaactcaaa
      841 atatacaagg tggtgcttct aaatgaaaga acaatcagca gcaggaattc tcgaaacaac
      901 aatcgaaaag gtaaaaaatc tcgtcaacgt aagcacaata atcggtgagc cgatgaatat
      961 tgacggcggc attacaataa ttccggtgtc taaggtgact tacggctttg cttcgggcgg
     1021 ttccgatttc ccctccaaga caaataaaga gattttcggc ggcggcggcg gagccggcgt
     1081 taccataact cccgttgctt tcctcgttat ttccgacgga gaggttacaa ttaagcatat
     1141 cacagcattt gacaatgctg ccgaaagagt tgttaatctt gttcccgaaa tgttcgataa
     1201 ggttacttcc gttgttaata aggcaaagaa ggaaaaggaa aaagcagctt ctaatgatgc
     1261 cgcggagtat gtttccgcag ttcccgcaga ggatgttcct tccgagaaca aataagcgtt
     1321 tctcgaattt cataataata aatctaccgc ctgcatattt taaaaatatg gcaggtggtt
     1381 gatttgtata aaagactttt ttgcgttttt ttatctgtgg cttttacggt actgtgcgca
     1441 tttccggctt atgccgaaaa cgatgagctt gatttaagcg cacgagcggc gattttaatg
     1501 acaagcgaca ctcttgaggt cgtgtatgca aaaaacgagc acgaaagatt gcccatggcg
     1561 agcacaacaa aaattatgtc ggcacttttg gctctcgaat acaaaagccc tgacgaggca
     1621 gtaacggtaa caaaagaggc tgtgtctgtc gagggaacgt ctatgggact tcttccggga
     1681 gactgcgttt cggtaaagac tcttgcgtgc ggtatgctgc tctcttccgg caatgatgcc
     1741 gcaaatgtcg cggcatacgc cgtgtcaggc ggcttggacg aatttacggc tcttatgaac
     1801 caaagggcta aagagcttaa tatgaacgat acttcgtttg aaacaccttc ggggcttgac
     1861 agcgaaaatc attattccac agcatacgat atggctcttc tcggagcaga ggcgattaaa
     1921 aatccggagt ttttaagcat ttgctccaaa aaaagcatga gcgtcagtta cggcaatccg
     1981 ccgtatcgca gaaccgttac aaaccacaat aggcttctta ccatagatga agactgcata
     2041 ggaattaaga cgggcttcac aaaaaagagc ggcagatgtc tcgtctcggc ggtgagacgc
     2101 ggcggcgtaa cgcttatagc agtcacgcta aatgcgccgg acgattggaa tgatcacgaa
     2161 aaaatgtata attacggctt ttccgtcgta aaaaatcgtt ctcttttctg tgatctttcg
     2221 ggcgtcaggc tgagtgttgt cggctcagat aaatcctctg tcggtgtaaa gcctttatac
     2281 acgccgtacc ttgcctcgct caaaaatatt gagcctgaaa tcaaaatttg cattaaaaat
     2341 tttgaatatg caccggttga gagcggcaaa aatgtaggta ccgtatatta tctgtatgac
     2401 ggaagagtat tatccgaggt gccgatagtg acttcggaag gtgccgaata tatatccgac
     2461 tccgaaaaga tgcaaaaaaa gtcgttatcg caaaagatta aaaaattttt taggaaagtg
     2521 ctttggtgaa aaatatgact gatgtgaaga tcagactgca aaaatttatg gctgagagcg
     2581 gcgtcgcttc aaggagaaaa tccgaggagc tgatagcggc aggtaaagtc aaggtaaacg
     2641 gcaagacagc ctgcgtggga gataaggtaa atcccaaaac cgacaaggtt gtggttgcgg
     2701 gcaaaaagct cagcgcgccg aaagaaaacg tctatattat gcttaataaa ccgagaggat
     2761 ttatcacaac aatgagcgac gagatggaca gaaaatgtgt tgcggagctt gtaagcgata
     2821 tagatgcgag aatatatccc gtcggcaggc ttgaccgtga aagcgagggc cttttgctta
     2881 tgacaaacga cggcgaattt gccaacgctt taactcaccc gtcaatgcac gtgccgaaga
     2941 cctatcgcgt tacaatacac ccgtctcttt caaaagagca aagagcggct cttgaaaacg
     3001 gcgtggaaat tgacggcaaa atgacgctgc cgtccgaagt ccgcgttgtc tcacgcgaag
     3061 agggtcgtgt ggttatcgaa ctcacgattt tcgagggcag aaaccgtcag ataagaaaaa
     3121 tgtgcgaaag cctcgggctt gaggtcgcaa ggctcaagcg cattaagata ggctccgtaa
     3181 agctcggaat gttaaagacg ggcgattacc gccaccttac gcccgacgaa gtgcatgcgc
     3241 ttatgacatc ggcggagcaa agcggtaatt tgaggcgcgg tttaaatgat aaaatttgag
     3301 tgtgttacgg aattatccga attgcaatgt ctctttcccg gagaaccgtt ttatgataac
     3361 gtgcgttacg ccgcgtataa aacagaatac ggcggtaatg atttttctct gctgttttct
     3421 ttaaacggaa atatctgtat tttagagcgt ataaaatcgg ataagaatga gccgtccgtt
     3481 actgacggac tgcttcgttc ggcgctcgat tttgcttttc gccgcggtgt tgatacggcg
     3541 gtttgcagcc tttttgagtt ttcggatgag cttatttctc tcggttttac gcaaaaagac
     3601 ggagtctttt cgggaaatac ggccgaactt ttgcagggaa gctgctgtaa acattgacaa
     3661 cgtcggttta aaatagtaaa atattataac taaaagcgag ggtaaccccg tcttattaaa
     3721 ttcggggttt cgctttattt tttatgcggc gtattttgtc cgcaatttaa acataggggc
     3781 aaccgatatg attaaaagta tgacaggtta cggcaagagc gagcagacaa tcgattcgct
     3841 caatgtgacc gttgaaataa aaagtgtaaa tcaccgttat tttgaatttt cggcaagagt
     3901 gccgagagag tacggttttc ttgaggaaaa gctcaaaaaa tactgtaatt cgcttataac
     3961 ccgcggaaaa gtcgagtgct atgtctcggt tgaggacctt gaagagcgcg agatggaggt
     4021 taatgtcaat gaaactctcg ccgccggcta tgtcaaagcg ttaaaagagc tttccgaaag
     4081 attcggcctc aaagacgata tatcggctgt aacgctttca cgctaccccg acgttataac
     4141 gcttcataaa gcgtccgagg acgaggagcg aatttggaat gcggtaaaga cagttgccga
     4201 aaccgccgtt tcaaagttta tcgaaatgcg tgagacggag ggcagcaagc tcagagggga
     4261 tatactttca agagctgatt atattatcga gtgcgtcgag ttcattgaag gccgttctcc
     4321 cgaaaccgta agagaataca acgaaaaatt gaagctgcgt atgaaagagc ttctcggcga
     4381 cgccgcggta gacgagcaaa ggcttttaaa tgaagccgca atttatgccg acaaaatagc
     4441 cgtcgatgaa gaaaccgtaa gacttcgcag tcatatttca cagcttcgtg aatttatgaa
     4501 ttcaagcgag gcaataggca gaaagcttga ttttcttgtt caggaaataa accgtgaggc
     4561 aaatacaata ggctctaagg cgcaggacgt cgatatagcc aaaaaggtca tcgccataaa
     4621 ggcggaggtc gaaaagattc gcgagcaggt tcaaaatatt gagtgaggaa gtcagcaatg
     4681 aagctaatca acatagattt cggaaatgcg gtaaacacag accgaatttt agcggtggca
     4741 ggccctgagt cggcgccgat aaaacgaata gcgcaagagg caaaagagca gaaaatgctc
     4801 attgacgcaa cacaggggcg aaaaacccga gccgtcataa ttaccgacag caaccatgtt
     4861 atattaacct atttgcaggc ggagaccatt atggctcgct ttaatgacga cagcgataag
     4921 gagcaggcat aatgga
//
LOCUS       DBMDVO010000016         4881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7200_length_4881_cov_1.347071, whole genome shotgun sequence.
ACCESSION   DBMDVO010000016 DBMDVO010000000
VERSION     DBMDVO010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7200_length_4881_cov_1.347071"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="ACZPE2_00460"
     CDS             complement(<1..1062)
                     /locus_tag="ACZPE2_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1318349.1"
                     /translation="MEQSKTSAGISAESAAKARSEISITRRYFADMLILMVVPAVMAW
                     YYYGGRAVRLMVLSVLTAVLCEAAGSFIFKAQASVSDLSAVATGLMTSLCMPASAPYY
                     LPCIASAFAVIVAKLPFGNARSHMFTPAAAGIAFVTICMPDKVFSYPAILSSGTTAVS
                     GGQGFFPGTSIAYMLSQKNSVGTGLLNYVDVLVGSVSGPMGATCSIALFGALLFLGVK
                     RPKNFTVSVSFLAVCFIYALLFPRVLTGRFVSAFMEICGGTLLFSSVFFLTDDKLLPK
                     SFYARICYGAAAGLMCMIMRSVGKFEDSTVFAVLLANGLVTAFDKLPLTKRERHLVAA
                     ENAKVRAALADKLEEKALEN"
     gene            1158..1526
                     /locus_tag="ACZPE2_00465"
     CDS             1158..1526
                     /locus_tag="ACZPE2_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318350.1"
                     /translation="MNIHKSRGDRVIVELSSNELSRYELCPEEMDCKNPKTRRLINDV
                     MKRAQRHIRRDITSFDKMNVDVMETDEGCIMILCFKGIKTKRPFSGYVCVLPQTDDIF
                     RLARGLFASPRKNKTGESFF"
     gene            complement(1676..2335)
                     /gene="rpe"
                     /locus_tag="ACZPE2_00470"
     CDS             complement(1676..2335)
                     /gene="rpe"
                     /locus_tag="ACZPE2_00470"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1318351.1"
                     /translation="MVKISPSILSSDYGNLESELKKMEECGADMIHVDVMDGHFVPNI
                     TLGAPIVKCIRKATKLTFDVHLMISDPYKYVPDFAKAGSDIITFHTESDSDTEKTIDE
                     ILSLGKKAGLSVKPGTPIEAIYPYLDKLSLVLIMTVEPGFGGQSFMEDMMPKVTAVKN
                     EIERRGLKDIDIEVDGGINKDTISVAAKAGANAFVSGSALFGSDDIKKTIADFKRSAT
                     I"
     gene            complement(2420..3262)
                     /locus_tag="ACZPE2_00475"
     CDS             complement(2420..3262)
                     /locus_tag="ACZPE2_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318352.1"
                     /translation="MSEANVNKKRKNRWAFPLGLVITVFAVIGLVCVILAGVNATKKA
                     VIKSKNIDEYNTMLTPVVMNDPDPFDDITKANKNQLIDISVWSILKSNLSPDKYEYGE
                     DGMIIPEEDVTAEFHKLFGTDTEPEHATVNGYGYTFTYDSAKHTYSIPLTGIVPTYTP
                     DVIKVQKKSGAVVLTVGYLAGDQWAQNSEGDMVAPEPDKYMKITLREKDGSYYINSIQ
                     ATSSPETATTAVQPVTEAPTEAPETTSSAEAASETQPVSDGTDASDTAAESDTASADT
                     AAAA"
     gene            complement(3278..4522)
                     /locus_tag="ACZPE2_00480"
     CDS             complement(3278..4522)
                     /locus_tag="ACZPE2_00480"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1318353.1"
                     /translation="MTDTELKCRNAKEAAKKLSTASANDKNKALLLIAEELKSNKDYI
                     LRENDKDMKAAEENGLPKVLLDRLLLSPDRIDGMAKGVIEVADLPDPVGKTLSDITRP
                     NGLRVKKVSVPLGVIAVIFEARPNVTSDAASLCLKSGNCSVLRGGKEAIHSNTAIFNV
                     MRAALKKSPLPEDCIQFITDISHESANELMTMNKYVDVLIPRGSARLIGTVVKNSTVP
                     VIETGVGNCHIYVDKDADLDMAADIIFNAKTSRPSVCNAAESLLIHKDVAEKALKLIK
                     KRLDEKNVELRGDKTSKEILPDIKEASEDDWGREYLDYIMSVKIVDNIDEAIEHIYKY
                     GTGHSECIVTENDEAANEFMSRVDAAAVYRNASTRFTDGGEFGFGAEIGISTQKLHAR
                     GPLGLPQLTSFKYEIFGNGQIR"
     gene            complement(4529..>4881)
                     /locus_tag="ACZPE2_00485"
     CDS             complement(4529..>4881)
                     /locus_tag="ACZPE2_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR1318354.1"
                     /translation="VYGDNDCLSAIVAKLIGADALIILTDIDGLYNGNPTVDEDAEII
                     PTVTEITDELIGFAGGHGSKFGTGGMVTKLHAAQIAMSAGIDMIVMNGSAPEDIYKAL
                     DGKQIGTFFVGKKD"
BASE COUNT         1110 a         1189 c         1249 g         1333 t
ORIGIN      
        1 gttttcgaga gccttttctt caagcttgtc cgcgagagct gcgcgcacct tggcgttttc
       61 tgccgcaaca agatgtcttt cgcgctttgt aagcggcagc ttgtcaaatg cggtgacaag
      121 tccgtttgca agcagtacgg caaagaccgt actgtcttcg aatttgccca cagagcgcat
      181 tatcatacac ataagccctg ctgccgcacc gtagcatatc cttgcgtaaa agctcttcgg
      241 aagaagcttg tcgtcggtca aaaagaatac cgatgaaaac aaaagcgttc ctccgcatat
      301 ttccataaat gccgagacga aacggcccgt cagcacgcgc ggaaacagga gcgcgtaaat
      361 aaaacagact gcgaggaagg atacggaaac ggtgaaattt ttcgggcgtt ttacgccaag
      421 gaacaggagt gctccgaaaa gtgctatcga gcaggttgcg cccatagggc cggaaaccga
      481 gcctacaagc acgtcaacat agtttaaaag gccggtgccg accgagtttt tttgcgagag
      541 catatatgcg atagacgtgc cggggaaaaa tccctgtcct cccgaaacgg cggtagtgcc
      601 ggacgagagt attgccggat aagaaaagac cttatccggc atgcatattg tgacaaaggc
      661 tatgcctgcc gctgccggcg taaacatatg agaacgcgca ttgccgaacg gcagctttgc
      721 gacgataacg gcaaaagccg aggctatgca cggcagatag tacggagcgg acgcgggcat
      781 acaaagtgat gtcataagac ccgtggcgac agccgagaga tcggaaacag aggcttgagc
      841 tttgaaaata aacgagcctg cggcttcgca gagcacggcc gtaagcacgg aaagcaccat
      901 aaggcgcacg gctctgccgc cgtagtaata ccaagccatc acggccggaa cgaccataag
      961 gataagcata tcggcaaagt atctgcgtgt tattgaaatt tcactgcgcg cttttgcggc
     1021 gctctcggcc gaaatgccgg cagatgtttt gctttgttcc aaaaaattca cctgttttcc
     1081 atattttatt cattgcgaaa caagcgttcc gacaatatga tgtaaagaga taatttttat
     1141 tttggggagg ctttcctatg aatatacata aaagccgtgg ggacagagtg atagtcgagc
     1201 tctcgtcaaa cgagctgtcg cgctatgagc tttgtcctga ggagatggac tgtaaaaatc
     1261 cgaaaaccag acgccttata aatgacgtaa tgaaaagagc acaaagacat attcgccgtg
     1321 acataacctc cttcgacaaa atgaacgtgg acgttatgga gacggacgaa gggtgtatta
     1381 tgattctctg ctttaagggc ataaaaacca aaaggccgtt ttcgggctac gtctgtgttt
     1441 tgccgcagac ggacgacatt ttcagacttg caagggggct ttttgcctca ccgcgaaaaa
     1501 ataagacagg cgagtctttt ttttgacggc aacagctatg ctgcctgcgt tctgccgtat
     1561 gcggtctttt caagagaaat attttttgtg ctcagcgaat tcggaaaccc ggtgagggcg
     1621 tctgtcggca caagagagta ccttttcgag cacaaaaagc ttataagccg cgattttata
     1681 tcgtcgctga cctcttgaag tcggctatgg tcttttttat gtcgtcgctg ccgaagagcg
     1741 cgcttccgga aacgaatgcg ttggcacctg cctttgcggc aaccgaaatt gtgtctttat
     1801 ttattccgcc gtcaacctca atgtcgatgt ccttgagtcc gcggcgttct atttcgtttt
     1861 taacagcggt gaccttgggc atcatatcct ccataaagct ttggccgccg aagccgggct
     1921 caaccgtcat aataagcacg agtgagagct tgtcaagata cggatatatc gcctctatcg
     1981 gagttccggg ctttaccgaa agtcccgcct ttttgccgag tgacaggatt tcgtctatcg
     2041 tcttttccgt atcggagtcc gactccgtat ggaatgtgat tatgtcggag cccgcctttg
     2101 cgaagtccgg cacgtattta taagggtcgc ttatcataag gtgcacgtca aaggtaagct
     2161 ttgtcgcttt tcttatgcac tttactatcg gcgcgccgag cgtgatgttc gggacaaaat
     2221 gcccgtccat aacgtctacg tgaatcatat ccgcgccgca ttcctccatt tttttaagtt
     2281 cgctctcaag attgccgtaa tcgcttgaga gaattgacgg tgatattttt accataattt
     2341 ctccttttta taacgctgtc ggtttccaat cagaaagccg aataatctta acacaaagtc
     2401 tttagttaaa aattaactgt cacgccgccg cagcggtgtc tgccgaagcc gtatcgctct
     2461 cggctgcggt gtcagacgcg tcggtgccgt ctgagacggg ctgagtttca ctcgcagcct
     2521 ctgcggaaga ggtggtttcg ggtgcttctg tcggagcctc ggtgacgggc tgaacggcgg
     2581 tggttgccgt ctcgggcgaa gatgtagcct gaattgaatt tatataatag ctgccgtctt
     2641 tttcccttaa agtgattttc atatatttgt cgggctcggg agctaccata tcgccttcgc
     2701 tgttttgtgc ccattggtct cctgcaaggt atccgacggt caaaaccacg gcgccgcttt
     2761 ttttctgaac ctttataacg tcgggcgtat aggtagggac aataccggta agtggtatag
     2821 agtacgtgtg ctttgccgaa tcatatgtga acgtgtagcc gtagccgttt accgtggcgt
     2881 gctcgggttc ggtgtccgta ccgaaaagct tgtgaaattc ggcggttacg tcctcctcgg
     2941 gaattatcat accgtcttcg ccgtactcgt atttgtccgg cgagaggttg ctctttaaaa
     3001 tagaccaaac ggaaatgtca ataagctgat ttttatttgc ctttgtgatg tcgtcaaacg
     3061 ggtcggggtc gttcatcacg acaggcgtca gcatggtatt gtattcgtct atgtttttgc
     3121 ttttgattac cgcttttttt gtggcgttta ctccggcgag aattacgcag acaagaccta
     3181 ttacggcaaa taccgttatt acaaggccca gggggaacgc ccatcggttt ttacgctttt
     3241 tatttacatt agcctcagac attctgactc tcctaactta tcttatctga ccgtttccga
     3301 aaatttcgta tttaaacgat gtgagctgcg gcagaccgag agggcctctc gcgtggagct
     3361 tttgcgtgga tatgcctatt tccgctccga agccgaattc gccgccgtcg gtaaagcgcg
     3421 tgctggcatt gcgatagacc gccgccgcgt ccactctcga cataaattcg tttgccgcct
     3481 cgtcattttc ggtgacgatg cactcgctgt gacccgttcc gtatttatag atatgctcta
     3541 tggcctcgtc gatattatct actatcttta ccgacattat gtagtcgagg tactcgcgac
     3601 cccagtcgtc ctcggaggct tcctttatat cggggagtat ctctttagat gtcttgtcgc
     3661 cgcgcagctc gacatttttt tcgtcaagtc gctttttgat gagcttcagc gccttttcgg
     3721 caacgtcctt gtgaattaaa aggctctcgg ctgcgttgca tacggacggg cgcgatgtct
     3781 tggcgttaaa tatgatgtcg gccgccatat cgaggtcggc gtccttatcg acgtaaatat
     3841 ggcagttgcc gacgcccgtc tctataacgg ggacggtgga gtttttgacc accgtgccta
     3901 taagccttgc gctgccgcgc ggaatcagca cgtctacata tttgttcatg gtcataagct
     3961 cgttggcact ctcgtgagaa atgtcggtta taaactgtat gcagtcctcc ggtaagggcg
     4021 attttttgag ggcggcgcgc attacattaa atattgcggt gttcgagtgt atcgcctcct
     4081 tgccgccgcg aagcacggag cagttgcccg atttcaggca aagcgaagcc gcatcggacg
     4141 taacgttggg gcgagcctca aatataacgg cgatgactcc gagaggcacc gaaacttttt
     4201 tgacccgaag gccgttcggc cttgttatat cggagagtgt tttaccgacg gggtcgggca
     4261 ggtcggcaac ctcgataacg ccctttgcca taccgtctat cctgtcgggg gagagaagaa
     4321 gtctgtccaa aagaaccttg ggtaatccgt tctcctcggc agccttcata tccttatcgt
     4381 tttcgcggag aatataatcc ttgttacttt taagctcttc ggctataagt aaaagagcct
     4441 tgtttttatc gtttgccgag gctgttgaga gctttttcgc agcctccttt gcgtttcggc
     4501 atttaagctc ggtatcagtc attttgtctc aatccttttt acctacaaaa aatgttccta
     4561 tttgcttgcc gtcgagagcc ttgtatatgt cctcgggagc ggagccgttc ataactatca
     4621 tatctattcc cgcgctcatg gctatttgag ccgcgtgtag ctttgttacc attccgccgg
     4681 tgccgaattt tgagccgtga ccgcccgcaa agcctataag ctcgtctgta atctcggtta
     4741 cggtgggtat tatttcggcg tcctcgtcga ctgtggggtt accgttataa agcccgtcta
     4801 tgtcggtgag gattatcagc gcgtcggctc ctatgagctt tgcgactatc gccgagagac
     4861 agtcattgtc gccgtagaca a
//
LOCUS       DBMDVO010000017         4842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7300_length_4842_cov_1.195950, whole genome shotgun sequence.
ACCESSION   DBMDVO010000017 DBMDVO010000000
VERSION     DBMDVO010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_4842_cov_1.195950"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="ACZPE2_00490"
     CDS             complement(<1..316)
                     /locus_tag="ACZPE2_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1318355.1"
                     /translation="MEYFAKEYDVAVIGAGHAGIEAGLASARLGSKTIVFTINLDWIG
                     NMPCNPSIGGTSKGHLVREIDALGGEMGKAADKNEIQSRMLNRGKGPAVHSLRAQIDR
                     REY"
     gene            complement(329..1690)
                     /gene="mnmE"
                     /locus_tag="ACZPE2_00495"
     CDS             complement(329..1690)
                     /gene="mnmE"
                     /locus_tag="ACZPE2_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1318356.1"
                     /translation="MSTIAAISTGLAAGGIGIVRISGENALNVADSIFKTVQGIELKK
                     LSGYKAAFGRVFFDGKPIDEAVALVFRAPKSYTGEDVVEISCHGGLYITKQVLRAALK
                     NGAVPAEPGEFTKRAFLNGKTDLTKAEAVMGIISAHGKEAGEAAFSALEGALYKEIHK
                     ITDVLLKINAALAAWVDYPDDEIEDLTDESILDAVTSSKAALKELLNRFDAGQAITEG
                     TETAIVGRPNVGKSTLMNMLTGTEKSIVTSVAGTTRDIVEETVTIGNVTLRLADTAGL
                     RETDDTVESIGVERAKRKIAAAKLIIAVFDSSCALSDEDREIFDLCSGKSCLAVINKT
                     DLEPKIDEDEIKRNFENVVYISAKDNSGLDLLRETIEKILGTANFDTTAALLMNERQL
                     SCCEKAYSALCEAENAEKTGVTRDAIQVCIDSAIENLMVLTGEKATESVVNEIFSQFC
                     VGK"
     gene            complement(1943..2920)
                     /gene="jag"
                     /locus_tag="ACZPE2_00500"
     CDS             complement(1943..2920)
                     /gene="jag"
                     /locus_tag="ACZPE2_00500"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR1318357.1"
                     /translation="MIKESVKSASTIEDAKAAALLELGLTEDDVYNIEVLQMPQKKTF
                     GIFGGKPAEVKVWVDVPEKKEKPVSEKNTYKKPASAPKKVEDRKKEAKKENTRKVEKE
                     EKTEEEKKASLKETNEYEKTASYIAEIVKGLGLPECSVKTFADDEEIVFELDCGDDYG
                     IVIGRRGETLDAIQYLARMVANRGVSGYKRVSINIGNYREKRKGALVSLAKKDAARAV
                     RTGRNITLEPMNPYERRIIHTAVQEVEGATSFSVGQDLDRRVVIAPENGGKGSYGDRN
                     YRRGRGGRNDRRPKREPYVPQVAPDREQKNDAAGVSVYGKIEIKKNPEE"
     assembly_gap    2992..3001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3002..3896)
                     /locus_tag="ACZPE2_00505"
     CDS             complement(<3002..3896)
                     /locus_tag="ACZPE2_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1318358.1"
                     /translation="MNTSKIYEILGIPFGFVISLFYAMTDNYLLSIVCLMIIVKLCLL
                     PSSVKQQKAQAKQARLQPKLRRIQKKYEGNQQKIQEETQNLYSREGFSAMGGGCMPMI
                     IQLIVMTGLFQVNYHPFSMVLNIRGDVLSSIKEAVMPLLTETEAKGASFRIELYALQH
                     WSDIRNSVAGLTPEMISKVDLFLQRFTVFGLVLSHTPNFRVFDVYWAIPIITGVLSLA
                     MSVYSLLRQKKLNPEMSKNPTMGCMMLFTPAMQIYFAFLFPTVVGIYVIMSTVVSFAQ
                     MVVLNITHSPQKVLAKLMVEET"
     gene            complement(3933..4166)
                     /gene="yidD"
                     /locus_tag="ACZPE2_00510"
     CDS             complement(3933..4166)
                     /gene="yidD"
                     /locus_tag="ACZPE2_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1318359.1"
                     /translation="MKELAIKMIRFYQRKISPLFPPRCRYYPTCSQYAVTAIERFGII
                     RGGLLAVWRLLRCNPLFPGGLDPVPEKRNKKKG"
     gene            complement(4163..4501)
                     /gene="rnpA"
                     /locus_tag="ACZPE2_00515"
     CDS             complement(4163..4501)
                     /gene="rnpA"
                     /locus_tag="ACZPE2_00515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1318360.1"
                     /translation="MKNTVTLNRNCDFLRLYGRGKVYTDPALVIYLLKNRAGICRIGI
                     TVSKKTGNAVQRNRSKRIIRAAYSAVEESICGNWDFVFVARGKTKYLKSTDIQKVLTE
                     QLLKAGAIKK"
     gene            complement(4514..4648)
                     /gene="rpmH"
                     /locus_tag="ACZPE2_00520"
     CDS             complement(4514..4648)
                     /gene="rpmH"
                     /locus_tag="ACZPE2_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1318361.1"
                     /translation="MLRTYQPKKRHRKMEHGFRKRMATKNGRKVLARRRAKGRKQLSY
                     "
BASE COUNT         1170 a         1168 c         1020 g         1474 t
ORIGIN      
        1 aatactccct gcggtcaatc tgcgcgcgca gcgaatgaac agccggacct tttccgcggt
       61 tgagcatacg cgactgtatc tcgtttttgt cggcagcttt tcccatttcg ccgccgagag
      121 cgtctatttc tcttacaaga tggccctttg aagtgccgcc tatcgacgga ttgcacggca
      181 tattgcctat ccaatcaagg ttaatggtaa aaactattgt ttttgagccg agccgagccg
      241 aggcaagacc tgcctcaata ccggcgtgac cggcgcctat aacggcaaca tcatattctt
      301 ttgcaaaata ttccataatt tatctccgtc acttgcctac acagaattgc gaaaaaatct
      361 catttaccac gctttcggtg gctttttcgc ctgtcaaaac cataagattt tctattgcgg
      421 agtctatgca gacttgaatt gcgtctcttg taacgcctgt tttctcggca ttttccgcct
      481 cacagagtgc cgaataggcc ttttcgcagc aggacagctg tctttcgttc atcaaaagag
      541 ccgcggtcgt gtcgaaattt gccgttccga gtattttttc gattgtctca cgaagtaaat
      601 cgagtcccga attatccttt gcggatatat aaactacatt ttcgaagttg cgttttattt
      661 catcttcgtc gattttcggc tccaaatccg tcttgtttat aacggcgaga cagcttttgc
      721 cggagcacaa atcgaaaatt tccctgtctt cgtcggagag cgcgcaggac gagtcaaaca
      781 cggcaatgat aagctttgca gcggcgattt ttcttttggc tcgctcaacg cctatactct
      841 caacggtgtc atctgtttcg cgcagacctg ccgtatccgc gagacgaagc gtaacgttgc
      901 ctatcgtaac ggtttcctca actatgtcgc gcgttgtgcc ggcaaccgac gttactatgg
      961 atttttcggt tcccgtaagc atattcataa gggttgattt gccgacgttc ggtctgccga
     1021 ctatcgccgt ttcggttccc tcggttatcg cctgacctgc gtcaaaacga tttaaaagtt
     1081 ctttaagagc tgcttttgac gaggtaactg cgtctaaaat gctctcgtct gtgaggtcct
     1141 cgatttcgtc gtcggggtaa tccacccacg ccgcaagagc cgcatttatc tttaaaagga
     1201 catcggtaat cttgtgtatt tctttgtaaa gggcaccctc aagtgctgaa aaagcggctt
     1261 cgcccgcctc cttgccgtga gccgaaataa tacccataac ggcctctgct tttgtgaggt
     1321 cggtctttcc gtttaagaac gcacgctttg taaattctcc cggctccgca ggaaccgcgc
     1381 cgtttttgag tgccgctctt aaaacctgct ttgttatgta aaggccgccg tggcaggaaa
     1441 tctccacaac gtcctcgccc gtatagcttt tcggtgctct gaatacgagc gcaacggcct
     1501 cgtcaatggg ttttccgtca aaaaataccc gtccgaacgc cgctttgtag ccgctcagtt
     1561 tttttaattc gataccttgt acggttttga agatgctatc ggctacattc agggcgtttt
     1621 cgcccgaaat tctgactatt cctatgcctc ctgcggcaag acctgttgaa atagccgcga
     1681 ttgtactcat aaaatcacca cttttatctg tttggctttg tattttctgc gaacagacga
     1741 aaaactctgc ccgccgcgcc gtctttaaaa tcaataaatt ctcagagctc ggctttgata
     1801 ttttaaaaag caaaagcacc gaaacatgct gtccgcatat ccggtgctct taaaatctga
     1861 ttatttgttt cgttaacgaa gttttcgaca aaaatgcggt tcaaatgtct aaaactcttt
     1921 cgataatgaa gaaaactcag aattattcct ctggattttt cttaatttcg atttttccgt
     1981 aaaccgaaac gcctgcagcg tcgttcttct gttcgcggtc cggagcaacc tgcggaacat
     2041 agggctcgcg cttgggtctg cggtcatttc ttccgccgcg gcctctgcga taatttctgt
     2101 cgccgtagct gcccttgccg ccgttttcgg gagcaattac aactctgcgg tcaaggtcct
     2161 gccctaccga gaacgaggtt gcaccctcaa cctcctgaac ggcagtatga ataattctgc
     2221 gctcataggg gttcatcggc tcaagggtta tatttctgcc tgtacgaacg gctcttgcag
     2281 cgtccttttt ggcaagagaa acaagagcac ccttgcgctt ttcacggtaa ttgccgatgt
     2341 ttatggaaac gcgcttatag cccgaaacac cgcggtttgc aaccattctt gcaaggtact
     2401 gtatagcgtc gagagtctcg ccgcgtctgc ctattactat gccgtaatca tcgccgcagt
     2461 caagctcaaa aacaatttcc tcgtcgtctg caaaggtttt aacgctgcat tcgggaagtc
     2521 cgagaccctt gacgatttcc gctatataag aagcggtttt ttcgtactcg tttgtctcct
     2581 tcaaagaagc ttttttctcc tcttcggtct tttcttcttt ttcgacctta cgggtgtttt
     2641 cctttttggc ttcctttttt ctgtcttcga cctttttggg agccgaagcg ggttttttgt
     2701 acgtattctt ttcggaaacg ggtttttcct ttttctcggg aacgtcaacc caaaccttta
     2761 cctcggcagg ctttccgccg aaaataccga aggtcttttt ctgcggcatc tgtaaaactt
     2821 ctatattata tacgtcgtcc tctgtaagac cgagctccaa aagggcggcg gccttagcgt
     2881 cctctatggt ggacgcggat tttaccgatt ctttaatcat ttatatacac cattcttttt
     2941 taagatttca tctcggctat ttttttgata ttagcctctt ttgagcgtcg cnnnnnnnnn
     3001 nctgtttcct caaccatcag ctttgcgaga accttctgcg gactgtgggt tatattgaga
     3061 accaccatct gcgcaaagga aacaacggta gacataatta catatatgcc gacaacagtc
     3121 gggaagagga atgcaaagta aatctgcatg gcaggtgtga aaagcatcat gcagcccatt
     3181 gtcggatttt ttgacatttc cgggtttaat tttttctgac gaagaaggga ataaacgctc
     3241 atggcaagcg acaaaacgcc ggttataatc ggtattgccc aataaacgtc aaataccctg
     3301 aaattcggag tgtgcgaaag gacaagtccg aaaacggtaa atctctgtaa gaataaatca
     3361 acctttgata tcatctcggg agtaagaccc gcaacggaat tacgaatgtc gctccaatgc
     3421 tgaagagcat aaagctctat tctgaacgaa gcaccctttg cctccgtctc ggtgagcaag
     3481 ggcataacgg cttccttaat tgaagagagc acatcgcctc tgatattcag aaccattgag
     3541 aaggggtgat agttgacctg gaaaagaccg gtcataacta taagctgtat aatcatcggc
     3601 atacagccgc cgcccatggc gctaaagccc tcgcggctgt acaggttttg tgtttcctcc
     3661 tgaattttct gctgattgcc ctcgtatttt ttctgaatac gtctgagctt cggctgaagc
     3721 cttgcctgct tagcctgagc cttttgctgc tttacggaag agggaagaag gcacagcttt
     3781 actatgatca taaggcaaac tatcgagaga agatagttgt ccgtcatggc atagaacagg
     3841 gaaatcacaa agccgaaagg aattccgaga atttcatata ttttacttgt gttcattaaa
     3901 ttcctccgaa gggagtaaaa gcttaatcaa acttagccct ttttcttatt tcgtttttcg
     3961 ggaacagggt caagtcctcc cggaaacaga ggattgcacc ttaaaagacg ccatactgcg
     4021 agtaaaccgc cgcgaatgat accgaatctc tcaatagccg taacggcata ttgcgaacag
     4081 gtcgggtaat atctgcatct cggcggaaaa agcggggaga tcttacgctg ataaaatctt
     4141 atcattttaa ttgcaagctc tttcatttct ttatggctcc cgcttttagg agttgttctg
     4201 tcaagacttt ttgaatatcg gtacttttca ggtattttgt tttgcctcgc gcaacaaaaa
     4261 caaaatccca atttccgcaa atactttctt caacagccga atacgccgct ctgattatcc
     4321 tctttgagcg atttctctgt acggcgttgc cggtcttttt gcttacggtt atacctatac
     4381 gacaaattcc cgcacggttt tttaataaat aaataaccag tgcaggatcc gtataaacct
     4441 ttcctctgcc gtagaggcgg aggaaatcac agttacgatt tagtgttacc gtatttttca
     4501 aaatgcttac cttttaataa gataactgct ttctgccctt agcgcggcgt cttgcaagca
     4561 cttttctgcc gtttttggtg gccattcttt ttctgaaacc gtgttccatc ttgcgatggc
     4621 gtttcttagg ctgataagtt ctaagcatta ttttttcgct cctttactct gaaataatct
     4681 gcgagaagtt attatatact aaaatccggc ttgttgtcaa ctttttttta atatttacgc
     4741 ttcttcaaaa gccctatata tatatataca tttataatta ctccatttat tttaagaaaa
     4801 tattgaaaaa atattaaaaa aatatggcat ttttgaagaa aa
//
LOCUS       DBMDVO010000018         4789 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7419_length_4789_cov_1.491681, whole genome shotgun sequence.
ACCESSION   DBMDVO010000018 DBMDVO010000000
VERSION     DBMDVO010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_4789_cov_1.491681"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(115..342)
                     /locus_tag="ACZPE2_00525"
     CDS             complement(115..342)
                     /locus_tag="ACZPE2_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318362.1"
                     /translation="MAEKRVRIKLPYVPGVLEDDKFVGDNGKTYLIKRGEYVEVPESV
                     VEILDGAEKFELEGAETRRKLQETAKTKALD"
     gene            complement(357..1376)
                     /locus_tag="ACZPE2_00530"
     CDS             complement(357..1376)
                     /locus_tag="ACZPE2_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HIR1318363.1"
                     /translation="MFTFNLQRFAVLTPTVGTYGVSNLNGTTNASTGNNLSAEMKTFY
                     NTALLENARAKYVFNQFGKKQTLKPGSGKNVEWRKFKTFAAATTPLTEGLIPDGNKLD
                     VSTITATVAQYGDYTPLTDVVMWAAVDPLISETVNEHADQAARTLDILTRDVLLTNTN
                     VYRVGGAADINSLTVANGKLTATDVARAATILKQQNTPTINGEYIAIIHPSVAYDLRQ
                     DSGWVEAQKYANPEKIYNGEIGKLHGVRFIETTEAKIQMNTATSAIAVYHSFFFGKDA
                     FGVVEPTGMTMETMIKSKEQIGGPLEQFGTVGWKAMHVSKVLYPERLLDVVSASTFSA
                     TDSAN"
     gene            complement(1404..2273)
                     /locus_tag="ACZPE2_00535"
     CDS             complement(1404..2273)
                     /locus_tag="ACZPE2_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318364.1"
                     /translation="MKKFDLQLFAEESAGEATGAVMSTADAAGMPVADGEAAGAQAVD
                     SAGAGTPTADERLPYDRIREMYAEDINKTVKNAVEKRLKREKDAQGTLKKLQPLVDRA
                     AKKYSKDAKDIDAIMAAVSADRSYYEELAERNGTTPEIEEMLERSAAEARSAREALEE
                     REEADRNREFAEKIGRQVLEVRERFPDFDLQRELDSNPQFAKLAQNPSLSLLEAYKAT
                     RFDDEIAKAMQNTAHAVEANVVNNIRSGAQVAENGLGNTPPATVKKDISKLSLSEIQE
                     YIERARRGEVVTF"
     gene            complement(2329..2589)
                     /locus_tag="ACZPE2_00540"
     CDS             complement(2329..2589)
                     /locus_tag="ACZPE2_00540"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1318365.1"
                     /translation="MIEVIYDRDVPSISASGHAGYAPAGQDIVCAGVSALLETLAMYA
                     TDVHDKGNFVISGGDTSLYDFAAGGIAEISEKYPENVIITYV"
     gene            complement(2586..4481)
                     /locus_tag="ACZPE2_00545"
     CDS             complement(2586..4481)
                     /locus_tag="ACZPE2_00545"
                     /inference="COORDINATES: protein motif:HMM:NF027825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR1318366.1"
                     /translation="MFNLRKKEDSVKNMPIGRDEVRKAAEILSKYKSAKSHLEARIIE
                     NEQFWKLKQWQSYNEVSKHDMRLASAWLWNTVVSKRADYMDAFPEPNILPRMPDDEAE
                     AQRLTDVIPVILDQNGFKEVYRDVVTYKLKQGTGVYGVFWDSSKHNGVGDIAIKKMDL
                     LRLFWEPGVIDIQDSANVFVVDLVDNDVLQQIYPQLRDKLKSNESLVSKYIYDDTVDA
                     TNKSCVVDWYYKKRSGYKSVLHFCKFVGDEVLYATENDTELPKHYITDEYGELTEQPY
                     GKPRSERGWYEHGRYPFVFDTLFDIEGSIAGYGYFDYGKDTQKEIDIINQAIVKNTVC
                     GAKPRYFIKENGAINESEFADWQNDFIHTGGSLGQDSIRPVDYSSLSGNYITFLDYKT
                     GELKEVTGNRDVNNGGTSGATAASAIAAMQEAGSKQSRDGISATYEAYKQVIYLVIEL
                     MREKYDTMRYFRIQGQSGEPQYVEYSNADLRAQPQMSGTGADMGLRLPEFDIDVTAEK
                     ASEYTKMAQNELALQLYGLGVFNPQNVDQSLMLLDAMDFYGKDDIVQKISANGTMLDQ
                     LMQYQQIALTLAQKYEPQTAAMIANGIQQSAPQMQQASVSVSGNAAEDTRMQRARDAA
                     QERTQPR"
     gene            complement(4474..>4789)
                     /locus_tag="ACZPE2_00550"
     CDS             complement(4474..>4789)
                     /locus_tag="ACZPE2_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Terminase-like family protein"
                     /protein_id="HIR1318367.1"
                     /translation="FEPEINDRISGWMQMQYRLAFDESGKPMMYVFRNCKDFIRTIPL
                     LQYSETKPEDLDTDGEDHIADETRYFLMSRPISPVPAKRKEFCVFVDPLDTATHMEVK
                     RV"
BASE COUNT         1006 a         1266 c         1138 g         1379 t
ORIGIN      
        1 acttaccgcg ggtaagcgct cttgtctgag caataacatc attgattttc atgtcgcacc
       61 tccatgataa aagtataaga aaaaaacggg ggtaagtgat acccccgttt tcagttagtc
      121 cagcgccttg gttttcgctg tttcttgcag tttgcgcctt gtttcagcgc cttcaagctc
      181 gaatttttcc gcaccgtcca gtatctcgac gacactttcg ggcacttcta catattcacc
      241 tcgcttgatg agatatgttt tgccgttgtc tccgacaaac ttatcatctt caagcactcc
      301 gggaacatag ggaagtttga ttctgactct tttttctgcc atgttaaaag ctccttttag
      361 tttgcactat ccgttgcaga gaacgtagaa gcagagacaa cgtcgagaag tctctcggga
      421 taaagaacct ttgagacatg catagccttc cagccgacag taccgaactg ctcaagaggg
      481 ccgcctatct gctctttaga cttgatcatg gtctccatgg tcatgccggt aggttcaaca
      541 acgccgaaag cgtctttgcc gaagaagaat gagtgatata cggcaatggc ggaagtagcg
      601 gtgttcatct gaatctttgc ctcggtagtc tcgatgaaac gcacgccgtg cagtttgcct
      661 atctcgccgt tgtatatctt ttcggggttg gcgtatttct gtgcctctac ccatccgctg
      721 tcctgtctaa ggtcatatgc aacggaagga tgaattatag caatatactc gccgtttata
      781 gtgggagtat tctgctgctt gagtattgtt gccgctcttg caacatctgt agcggtaagc
      841 ttaccgtttg caacagtaag cgagtttatg tcggcagcac cgccgacacg gtacacgttg
      901 gtgttggtaa gcagcacgtc acgtgtaagt atatcgagag tacgcgccgc ctgatccgca
      961 tgctcgttga ccgtctcgga aatcaggggg tcaactgcag cccacattac cacgtcagtg
     1021 agaggagtgt aatctccgta ctgtgcaacg gttgccgtaa tggttgatac atcaagcttg
     1081 ttgccgtcgg ggataagtcc ctcggtgaga ggcgtagtag cagccgcgaa tgtcttaaac
     1141 ttacgccatt caacgttttt tccgctgccg ggcttaaggg tctgcttttt gccgaactgg
     1201 ttgaacacat actttgcgcg tgcattttcg agcagagcag tgttgtaaaa ggttttcatt
     1261 tcggcagaca gattgttgcc tgtcgatgcg ttggtcgtac cgttaaggtt tgacacaccg
     1321 tatgtaccta cagtaggtgt aagaactgcg aatctctgaa gattaaaagt aaacataatt
     1381 tttccctttc tgcggggaaa agctcagaat gttacaactt ctcctcgcct tgctctctct
     1441 atgtattcct gtatttccga gagagacagt tttgaaatat cttttttaac tgttgcagga
     1501 ggtgtgtttc cgagaccgtt ttccgctacc tgtgcgccgc ttcggatgtt gttcactaca
     1561 ttcgcttcca cagcatgagc ggtgttttgc atcgcttttg ctatttcgtc gtcaaagcgg
     1621 gttgctttgt atgcttcgag gagtgacagc gacgggttct gcgcaagctt ggcaaactga
     1681 ggattcgagt ccaattccct ctgcagatcg aaatcgggaa acctttctct cacttccaat
     1741 acctgccgtc ctattttttc ggcaaattca cggtttctgt ccgcttcctc ccgttcttcg
     1801 agagcttcgc gggcgcttct tgcctctgct gccgagcgtt caagcatttc ctcaatctcg
     1861 ggggttgtgc cgtttctttc ggcaagctcc tcgtaatagc ttctgtcggc acttacggca
     1921 gccataatag cgtcaatgtc ctttgcatcc ttgctgtact ttttggcagc gcggtctaca
     1981 aggggctgca gctttttaag cgtgccctgt gcgtcctttt cccgcttgag gcgtttctca
     2041 acagcatttt ttaccgtctt gttgatgtcc tccgcataca tttcgcgtat acggtcgtag
     2101 ggaagtcttt catccgccgt gggagtccct gctccggcag agtcgactgc ctgtgctccc
     2161 gcagcttcgc cgtcggcgac cggcataccg gcagcatctg cggtactcat aaccgcacct
     2221 gtcgcctcgc ctgcgctttc ctccgcgaaa agctgtaaat cgaatttctt cataaaaatt
     2281 cctttctccg ggttaagccc gtgactctgt gtagattata aagagatatc acacataggt
     2341 gataatcacg ttttcgggat atttttccga aatttcggca attcctccgg cggcgaaatc
     2401 gtaaagagaa gtatctccgc cggatattac aaaattgccc ttgtcatgta catccgttgc
     2461 atacattgca agagtttcca gcagcgccga cactcccgcg cagactatat cctgcccggc
     2521 gggagcatac cctgcatgac cgctcgccga tattgacggc acatcacggt cgtagataac
     2581 ctctatcatc tcggctgcgt cctctcctgt gcggcatcac gcgccctttg catgcgtgta
     2641 tcctccgccg cattgcccga tacgcttaca ctcgcctgct gcatctgcgg agctgactgc
     2701 tgtatgccgt ttgctatcat cgcagctgtc tgaggctcgt atttctgtgc cagtgtcagc
     2761 gcaatctgct gatactgcat aagctgatca agcatagtgc cgtttgcgct gattttctgc
     2821 acaatatcat ccttgccgta aaaatccatc gcatcgagca gcataaggct ctggtcgacg
     2881 ttttgcggat taaatacgcc aagcccgtaa agctgcaggg caagctcatt ctgcgccatt
     2941 ttggtgtact cgcttgcttt ttccgccgta acgtcaatgt caaattcggg gaggcgcaga
     3001 cccatgtcgg ctcctgtccc gctcatctga ggctgtgctc tcagatcggc gttggaatac
     3061 tctacatact gaggctcgcc gctctgcccc tgtatgcgga aataccgcat tgtgtcgtat
     3121 ttctcccgca tgagctcaat aacgagatat atgacctgct tgtatgcctc gtatgtggca
     3181 gatataccgt cacggctctg ctttgagccc gcctcctgca tagcagctat agcgctggcg
     3241 gctgtggctc cgcttgtacc gccgttgtta acgtctcggt tgccggttac ttctttgagc
     3301 tcacccgttt tgtaatccaa aaacgtaata taattgccgc tgagagaact gtaatcaaca
     3361 ggtcttatgc tgtcctgtcc gagagagcct ccagtgtgga taaaatcatt ctgccagtcg
     3421 gcaaattccg actcgtttat cgctccgttt tcttttataa agtatcgcgg ctttgctccg
     3481 cagacagtat ttttgactat agcctgattg ataatgtcga tctctttttg cgtgtccttg
     3541 ccgtagtcaa agtatccgta tccggcaatg ctgccctcta tgtcaaagag agtgtcaaaa
     3601 acaaagggat atctgccgtg ctcataccat ccgcgctcac tgcgaggctt gccgtagggc
     3661 tgctcggtca gctcgccgta ctcgtccgta atgtaatgct tgggcagctc cgtatcgttt
     3721 tcggtggcat acaaaacctc gtcgcccaca aatttgcaaa aatgcaaaac cgatttgtag
     3781 cctgaccttt ttttgtagta ccagtccaca acacaagact tgtttgttgc gtcaaccgta
     3841 tcgtcatata tgtactttga tacaaggctc tcgttggact tgagcttgtc cctgagctgc
     3901 ggatatatct gctgcagcac atcattgtca accaggtcaa cgacaaaaac gtttgcgctg
     3961 tcctgtatgt cgattacgcc gggctcccaa aacaggcgca gcagatccat ttttttgatt
     4021 gcaatgtcgc ctacgccgtt gtgcttggac gagtcccaaa atacaccgta tactccggtg
     4081 ccctgcttga gcttgtaggt aacaacgtcg cggtatacct ccttaaatcc gttttggtcg
     4141 agtataacgg ggataacatc ggtaagccgc tgtgcctccg cctcatcatc cggcatacgc
     4201 ggcagtatgt tgggctcggg aaaagcgtcc atataatcag cccgctttga taccacggtg
     4261 ttccagagcc acgcggaggc gaggcgcatg tcatgcttgg acacctcgtt gtagctctgc
     4321 cattgcttga gcttccaaaa ctgctcattt tcgatgatcc tcgcctcaag gtgcgatttg
     4381 gcgctcttgt actttgacag tatttcggct gccttgcgca cctcgtctct gcctatgggc
     4441 atatttttaa cactatcctc ttttttgcgt aaattaaaca cgttttacct ccatgtgtgt
     4501 agctgtgtcg agcgggtcga caaatacaca aaattccttg cgctttgccg ggacggggga
     4561 tatcgggcgc gacatgagga aataccgcgt ttcgtcggct atatggtcct caccgtccgt
     4621 gtccaagtcc tcgggcttgg tctcgctgta ctgcaacagc ggtatagtcc gtataaaatc
     4681 cttgcagtta cggaaaacat acatcatcgg cttgccgctc tcgtcaaaag cgagtctgta
     4741 ctgcatctgc atccagccgg atatacggtc gttaatctcc ggctcaaac
//
LOCUS       DBMDVO010000019         4745 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7520_length_4745_cov_1.457364, whole genome shotgun sequence.
ACCESSION   DBMDVO010000019 DBMDVO010000000
VERSION     DBMDVO010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7520_length_4745_cov_1.457364"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..141)
                     /locus_tag="ACZPE2_00555"
     tRNA            complement(68..141)
                     /locus_tag="ACZPE2_00555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Gly,seq:ccc)
     gene            complement(244..750)
                     /gene="bilS"
                     /locus_tag="ACZPE2_00560"
     CDS             complement(244..750)
                     /gene="bilS"
                     /locus_tag="ACZPE2_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR1318368.1"
                     /translation="MTDKSYSIIFSSPTGNTRLLADAIRDALPEENCNYFGVSENADT
                     QSDILFIGFWTDKGIADKATLDLLEKLKNKRIFLFGTAGFGGDEEYFKEILANTKKSI
                     SGSNITVGEYMCQGKMPQTVRERYIKMKSMPNPAPNLDMLIENFDRALSHPDENDLKR
                     LRLSVEKL"
     gene            complement(984..2753)
                     /locus_tag="ACZPE2_00565"
     CDS             complement(984..2753)
                     /locus_tag="ACZPE2_00565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR1318369.1"
                     /translation="MSTKPYKGFEPKWMLQRAPENSYRSIFRWGDPDFFKYPKESLYK
                     YVKKRCNLKDEEFMQYNDDLGLDPVNLGEEHAPKIDKKHVKAIAAIVGEKNVSQSDYD
                     RLAVSYGQTMYDLLRMRHRRFDSLPDLVVFPETSEQIEKIVAYVTKNKIPLYVYGGGS
                     SVTRGVEPINGGVSLDMRRNFNKVIKFNETDQTITVQAGMSGPKLEETLQNAQSLFGA
                     KRPYTCGHFPQSFEYSSVGGWTVTRGAGQNSTYYGTIADIVVSQKYATPIGKIETSHY
                     PREATGPNLNQVMMGGEGTFGVLTEVTLKFFRWMPENRKRFSYMFKDWPTAMRAAREM
                     MQCECGFSSVFRLSDPEETSLMLHLYNVDETPLEPLLDKLGYKDFERCLFLGFTDGEK
                     GFSRNVAHNIMKIAVKHGGIPMTGYVCKGWEKGRFNDPYLRDTLMDFGFMIDTLECTV
                     NWSNMARVHREVREVCHKLPNTIVTTHMSHCYPQGANLYFIFMTKMQDEEEFRAYHAT
                     ILDAIQKSGAAISHHHGIGKMFAPWLEGYLGEKEYGIIRTLKNYFDPDYLMNPGGTIG
                     LDLKPEDKKYLREHTEYVDELKD"
     gene            complement(2756..4309)
                     /locus_tag="ACZPE2_00570"
     CDS             complement(2756..4309)
                     /locus_tag="ACZPE2_00570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1318370.1"
                     /translation="MEQSLVLTFDVGTQSARCLLVNESGDFEDKEQQKYKVPYLSKQP
                     GWAEQTPDFYFNIIAEMSKKVIERNADKLDRITAVCLTCIRDTVLCLDKDKRPLRDII
                     LWLDEREADEDGQIPAAKRALFSLVGMGESVDMIYRASTGNWIMQNEPEIWAKTDKYV
                     MLPSYLNYLLTGELKDSVANMIGHVPFDYKNRRWMKPGALTRCVCNVPSEKLCELVPS
                     GEVIGKITKDASEKTGIPIGLPLIATGSDKGCETIGMSVVKSNRAAISFGTTSTLQMA
                     VKKYFEPQPYMPAYPAVPNDMFNPEFEVYRGFWMISWFIEQFAAKDKVDAAEEGVCIE
                     RYLDDKIKDIEAGSGGLILQPFWTPGITNPNASGAIIGFSDFHTRYHLYRAIIEGICL
                     ELYHGLVKMQKRSSVTVDELYAGGGGSSSPVVCQIAADVFGLPVHRIQTHEASAVGCA
                     MVAFISQGVYGSYEDAIEHMVHKKDVFIPNEENHRTYMKIYGKAYSHLASRLKPIEKY
                     LHHFQTGGH"
     gene            complement(4334..4708)
                     /locus_tag="ACZPE2_00575"
     CDS             complement(4334..4708)
                     /locus_tag="ACZPE2_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1318371.1"
                     /translation="MTKELVCIVCPRSCRMTITSDGDELVVTGNTCKRGKEFAVSEMT
                     DPRRTVCTTVRTAYPSVPVLPVRVSGPIPKNRIFDLMHEVNRITVSKRLGREEVVVPN
                     ILNLGVDLIATSNILKDEKKQP"
BASE COUNT         1132 a         1151 c         1134 g         1328 t
ORIGIN      
        1 aaagcgagac gagagtaaaa tgtgaaaaag taaaaaacga caagcatcaa atgatacttg
       61 ccgtttttgg agcaggtgag gggaatcgaa cccccgtatt cagcttggga agctgatgtt
      121 ctgccattaa actacacctg cttacgtgct acgctgaata ttttatcatt atttttacaa
      181 taaatcaata gtaatatgat acgcggcaca gcttagtggg gtaggtcgcg ctgtcgcttc
      241 ttattacagc ttttccacgg agagcctcag ccttttcaaa tcgttttcgt cgggatgaga
      301 cagagcacgg tcaaagtttt caatcagcat atcgagattc ggcgcgggat tcggcatgct
      361 tttcattttg atatagcgct ccctgaccgt ttgcggcatt tttccctgac acatatactc
      421 accgactgtg atgttgctgc cgctaatgct cttttttgta tttgcgagga tttctttgaa
      481 gtactcttcg tctccgccga agcccgccgt tccgaaaaga aaaatccttt tatttttcag
      541 cttttcgagc aaatccaagg tcgccttgtc ggcgattccc ttatccgtcc aaaagcctat
      601 aaataaaatg tcggattgtg tatcggcgtt ttcgctcact ccgaaataat tgcagttttc
      661 ctccggcagc gcgtcgcgaa tggcgtcggc gagcagcctc gtgttgcctg tcgggctgct
      721 gaaaataatt gaatagcttt tatctgtcat gatgaacctc tttacttgtg cggcatatca
      781 catatgcggc gaaattatgt tataaattat tatataatta tgacggcaaa cagtcaacag
      841 ccgcaaatgc cgaaagctta tacaaaaagt aagcttttct gtgatcggca ataaaaaggc
      901 tttcgtttca ggtgagacga aagccttgat tttatatgct tatgcttgac gttacggctt
      961 tgagccgcgc ttcaacagcc ttttcagtcc ttgagctcgt caacatactc ggtatgctca
     1021 cgcagatact ttttatcctc gggtttaagg tcaaggccta tggttccgcc cggattcata
     1081 aggtaatcgg ggtcaaaata gtttttaagc gtgcggatta tgccgtattc cttttcgccg
     1141 aggtagccct caagccacgg ggcaaacatc tttccgatac cgtggtggtg gctgatggct
     1201 gcaccgctct tctgtatggc gtcgagaatt gttgcgtgat aagcacggaa ctcctcttcg
     1261 tcctgcattt tagtcataaa gatgaagtag aggttggcgc cctgcggata gcaatgcgac
     1321 atatgagttg taacgattgt gttcggaagc ttatggcaaa cctcacgaac ctcacggtga
     1381 acacgagcca tatttgacca gttgaccgtg cactcaagcg tatcaatcat aaagccgaaa
     1441 tccatgagtg tatcgcgaag atacgggtcg ttgaagcggc ccttttccca gcccttgcat
     1501 acgtagccgg tcatcgggat tccgccgtgc ttaacggcaa ttttcattat gttgtgcgca
     1561 acatttcttg agaagccctt ttcgccgtct gtaaatccga ggaagagaca gcgctcaaag
     1621 tccttgtagc ccaatttatc aagaagaggc tcaagcggcg tttcgtcaac attgtaaaga
     1681 tggagcatta atgaagtttc ctccgggtct gaaagacgga aaacagagga gaagccgcac
     1741 tcacactgca tcatttcacg agcggcgcgc atagctgtcg gccagtcctt gaacatataa
     1801 gaaaagcgtt ttctgttttc tggcatccag cggaaaaact tgagcgtgac ctcggtaaga
     1861 acgccgaagg tgccctcgcc gcccatcatg acctgattga gattcggtcc cgttgcctct
     1921 ctcggatagt gacttgtctc gattttacct atcggtgtgg cgtatttctg cgaaacaact
     1981 atatcggcta tggtgccgta ataggtgctg ttctgaccgg caccgcgtgt gacggtccaa
     2041 ccgccgacgg aggaatattc aaacgactgc gggaagtgac cgcaggtgta cggacgcttt
     2101 gcgccgaaaa ggctctgagc gttttgaagt gtttcctcga gctttggtcc ggacatacct
     2161 gcctgaacgg taatggtctg gtcggtctca ttgaatttaa tgaccttgtt gaagttgcgg
     2221 cgcatatcaa gagaaacgcc gccgttaatg ggctcaacgc ctcttgtaac cgacgagccg
     2281 ccgccgtaaa catagagcgg tattttattc tttgtgacgt aggctacgat tttctcaatc
     2341 tgctcgcttg tctccgggaa aacaacaagg tcgggaagag agtcaaagcg acggtgacgc
     2401 attctgagaa gatcgtacat tgtctgtccg taggaaacgg cgagacggtc gtagtcgctc
     2461 tgtgagacgt tcttttcgcc gactattgcg gcaatggcct ttacgtgctt tttgtcaatc
     2521 ttcggagcgt gctcttcgcc gagatttacg gggtcaagac ccaaatcgtc attatactgc
     2581 ataaactcct cgtccttgag attgcagcgt tttttaacat atttgtaaag gctctccttc
     2641 ggatatttga agaagtcggg gtcgccccag cggaaaattg aacgatagct gttctcaggt
     2701 gctctctgaa gcatccattt aggctcaaag cccttatacg gttttgtact cattatcagt
     2761 gtcctcctgt ttgaaaatga tgtaggtatt tttctattgg ctttaatctt gaggcaaggt
     2821 gagaatacgc cttgccgtaa attttcatat aggttcggtg gttttcctcg ttcggaataa
     2881 atacgtcttt tttatgtacc atatgctcta tggcgtcctc atagctgccg taaacgccct
     2941 gtgaaataaa cgcgaccatg gcacagccga ctgcgctcgc ctcgtgagtc tgaatacggt
     3001 gtacgggcaa gccgaacaca tctgcggcaa tttgacatac gaccgggctg ctggagccgc
     3061 ctccgccggc ataaagctca tctaccgtga cgcttgagcg tttttgcatt ttaacgagcc
     3121 cgtggtacag ctctaagcag ataccctcta ttatggcacg atacaaatga taacgcgtat
     3181 ggaaatccga aaagcctata attgcgccgc tcgcattcgg gttcgtgatg cccggagtcc
     3241 aaaacggctg taaaataagt ccgccggagc cggcctcaat atccttgatt ttatcgtcaa
     3301 gataacgctc tatgcaaacg ccctcctcgg cggcgtcaac cttatccttc gcggcaaact
     3361 gctctataaa ccacgaaatc atccaaaatc cgcgatagac ctcgaattcg ggattgaaca
     3421 tatcgttcgg gacagccgga taagccggca tataaggctg cggttcaaaa tattttttaa
     3481 ccgccatctg taaggttgag gtcgtgccga aggaaattgc tgctcggttt gattttacaa
     3541 cggacatacc gatggtttca cagcccttgt cggagccggt tgctataagc ggaagtccaa
     3601 ttgggatgcc cgttttttca gacgcatctt ttgtgatttt acctatgacc tcgcccgacg
     3661 gaacaagctc acagagcttt tcggacggaa cattgcacac gcagcgcgtg agagcaccgg
     3721 gtttcatcca acggcggttt ttgtaatcaa acggtacgtg acctatcata tttgccacgg
     3781 aatccttaag ctcgcccgtc aaaagatagt tgagatatga cggcagcata acgtatttgt
     3841 ctgttttggc ccatatttcg ggctcgtttt gcataatcca gttgccggta gaggctcggt
     3901 aaatcatatc taccgattct cccattccca caagcgaaaa caatgcgcgt tttgctgccg
     3961 gaatctgtcc gtcctcgtcg gcctcacgct cgtcgagcca taatattatg tcgcgaagcg
     4021 gacgtttgtc tttgtcgagg caaagcaccg tgtcacgtat gcaggtaagg catacggctg
     4081 ttatgcggtc aagcttatcg gcatttctct cgataacctt ttttgacatt tcggctataa
     4141 tgttaaaata aaaatccggg gtctgttctg cccatccggg ctgttttgat aaataaggaa
     4201 ccttgtattt ttgctgctcc ttatcctcaa agtctccact ctcattgaca agtaagcatc
     4261 ttgcactctg cgtgccgacg tcaaaggtca gcacaagtga ctgttccatt aaagattttc
     4321 ccctctttct tttttaaggt tgcttcttct cgtccttcag gatattgctt gttgcaatca
     4381 aatccacgcc gagattgaga atattcggta caacgacttc ctctcgtccg agtcgttttg
     4441 aaacggtgat acggtttacc tcgtgcatca ggtcgaaaat tctgtttttg ggaatcggcc
     4501 ccgatacgcg aacgggcaga accggtaccg aagggtaggc ggtgcgcacc gttgtgcaaa
     4561 cggtacgacg cgggtcggtc atttcgctta cggcgaattc tttgcccctt ttgcaggtgt
     4621 tgccggtcac aacaagctcg tcgccgtctg atgtaatcgt cattctgcac gaacggggac
     4681 aaacgatgca aaccaattct tttgtcattt tacaccgtct cctttaccga gtctttccag
     4741 ccgaa
//
LOCUS       DBMDVO010000020         4684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7669_length_4684_cov_1.155357, whole genome shotgun sequence.
ACCESSION   DBMDVO010000020 DBMDVO010000000
VERSION     DBMDVO010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7669_length_4684_cov_1.155357"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="ACZPE2_00580"
     CDS             complement(<1..946)
                     /locus_tag="ACZPE2_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1318372.1"
                     /translation="MDIKNILKELTSAVGVSGKETDAVLTAQRLLSEFGKTREDVLHS
                     AICEIEGDRAGHILLDAHIDRIGLIVTSVTDEGFLHVAACGGVDRRTLNGQEVTVYGS
                     EPVFGVIASTPPHLASGDNERKATEIDDIIIDIGAKNKQSAEKLVSPGDRAVVNSSFL
                     SLCGDSVSCGALDDRSGVAVLLRALELIKGKPHKKVTVVCSSREETSGGGAKAAAFNS
                     DADEIIAVDVSFAKTPDSKAEECGILGKGPMIGISPSLSYEFGEELVRVAEKNDIPYQ
                     LEVMGGRTGTNLDGMTTERGGIKSALISIPQKYMHTGIE"
     gene            complement(1094..2374)
                     /locus_tag="ACZPE2_00585"
     CDS             complement(1094..2374)
                     /locus_tag="ACZPE2_00585"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1318373.1"
                     /translation="MATPETLKKEILSASLDERLKKAYVTEENVKEQYDRYINLLNEF
                     EALFGKNRDVRLFSAPGRTEVGGNHTDHNHGRVLAAGINLDAIAAASKNDENIVRVKS
                     EGYSMDVVDAADLSVNPNEMGHSPALVRGVLAGFKKYGYKIGGFDATTASRVIAGSGL
                     SSSAAYEVLVGTMVNYLYNDGKVDAVTIAKIAQYAENEYFGKPCGLMDQMACSVGGFV
                     TIDFNDPKNPVIEEVDFDFAKCGHSLCIVDTKGSHSDLTDEYAAIRTEMESVAEFFGK
                     KVLREVSEEEFKSKIRELRPAVGDRAILRAMHFYADNARVLKEVDALRGGDFETFKKY
                     ILESGASSYKYNQNVFSVKQPSVQPVSLALAVSESILKGRGAWRVHGGGFAGTIQAFV
                     PNDLLKDYKSIMESIFGEGTCYVLYIRPVGGTEI"
     gene            complement(2377..3138)
                     /locus_tag="ACZPE2_00590"
     CDS             complement(2377..3138)
                     /locus_tag="ACZPE2_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1318374.1"
                     /translation="MTDRELTRRKLLNILACVLLILAVALLVLTFLMQINDVSSAYDE
                     VLERLAEFEEAVASIPSKWLVILVIFLIYIGKVLIPLPITAVCVIAGMVFPTPIAVAV
                     NFAGMTLLMTIKFYWGKHLGGGTIHKILTRYDNVRRMMESGKRSTDWILVLFRLVPYF
                     PINSISQLYGALEYDYWKFILLSLLGFTPKIISYSMIGRHVYNPFSLAFMLPIVIIFT
                     ISGVSILAVNKFIDIVANNKNKKTAETFSAGKDEK"
     gene            3329..4291
                     /locus_tag="ACZPE2_00595"
     CDS             3329..4291
                     /locus_tag="ACZPE2_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1318375.1"
                     /translation="MKTVILDGKTTNPGDLSWDFLKKYGEVEIFDRTPREKIVERTHD
                     ADIVVTNKTPLTRAEIDKMQNVKFVALLSTGYNVVDCAYLKEKGIPVSNIPAYSTMGV
                     AQLVFAFISELAVGVAMHSIAVKSGEWSNCPDFCFWEKPLTELSGKTIGIVGFGKIGG
                     AVADIAEAYKMNIIALSGHVTDQSARKNFRWAKNLDDLAENADIITFHCPLTKETEKI
                     VNADFLSKCKKTAFIINTSRGPVVDEKALADALNSGKIAGAGVDVLSTEPPAKDNPLL
                     TAKNCFITPHIAWAAFETRERLMGIFKSNIEAFVSGKPINVVNP"
     gene            complement(4399..>4684)
                     /locus_tag="ACZPE2_00600"
     CDS             complement(4399..>4684)
                     /locus_tag="ACZPE2_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318376.1"
                     /translation="TDKSTAMSRLTNDILTQSMVDSEIKNFISAAKINSATIEYTNIK
                     ATVVIEDGNLKEFKYSYDGNVAELNIKVAFISVNGKGAMHVEGAYTNFAY"
BASE COUNT         1216 a         1130 c         1093 g         1245 t
ORIGIN      
        1 cctctattcc ggtgtgcata tatttttggg gtattgatat aagcgcgctc ttaattccgc
       61 cgcgctccgt ggtcataccg tccaaattag tgccggttct gccgcccata acctcaagct
      121 gatacggaat gtcgtttttc tcggcaacgc gcacaagctc ctcgccgaat tcatatgaaa
      181 gcgacggtga aatacctatc atggggccct tgccgagtat tccgcattcc tccgccttgc
      241 tgtccggagt ctttgcaaag ctgacgtcaa ccgcaattat ctcgtcggca tccgaattaa
      301 atgccgcggc ttttgctccg ccgccgctcg tctcctcgcg agaggaacac acaacggtaa
      361 cttttttgtg cggcttaccc ttaataagct caagtgcgcg aagaagcacg gcaactcccg
      421 aacggtcgtc aagtgccccg caggacacgc tgtcgccgca gagcgagagg aaagaggaat
      481 tgacaaccgc tctgtctccc ggggaaacga gcttttccgc cgactgctta ttttttgcgc
      541 ctatatcaat aatgatgtca tcgatttccg tcgccttccg ctcgttgtca ccgcttgcaa
      601 ggtgcggcgg agtgcttgct ataactccga ataccggctc actgccgtaa acggtcacct
      661 cttggccgtt caatgtgcga cggtcaactc cgccgcaggc ggcaacgtgc aaaaacccct
      721 cgtcggtgac ggaggttacg ataagaccta tacggtctat atgagcgtca agcaggatat
      781 gccctgccct gtctccctcg atttcgcata ttgcggaatg aagaacgtcc tcgcgcgttt
      841 tgccgaattc ggacaaaagc ctttgcgccg ttaaaaccgc gtcggtttcc tttccggata
      901 caccgacagc cgaagtcagc tcttttaata tgttttttat atccattttt ctctgccttt
      961 cggacaattt gtcaacgtgt aaaacgctaa agcaaatgct gtaatcctat cgaaattttg
     1021 aattataatc gtcactcttt gtaggtggaa tatacctccc tttacaaggg agccttaggg
     1081 gaggcggcga aaatcagatt tcggtgccgc ctaccgggcg aatgtagagc acatagcagg
     1141 ttccctcgcc gaaaatgctc tccattatgc tcttataatc ctttaaaaga tcgttcggga
     1201 cgaaagcctg aattgtgccg gcaaatccgc cgccgtgaac gcgccatgcg ccgcgtccct
     1261 ttaagatgct ctcggaaaca gcgagagcga gagatacggg ctgaacggac ggctgtttta
     1321 cggaaaatac gttttgatta tatttgtatg atgatgcgcc gctctcgaga atatacttct
     1381 tgaaggtctc aaaatctccg ccgcgaagag cgtcaacctc ttttaacact cttgcgttgt
     1441 cggcatagaa atgcatagcg cgaagtatgg ctctgtcgcc tactgccgga cgcaattctc
     1501 ttatttttga tttaaattcc tcttcgctta cctcgcgaag aacctttttg ccgaaaaatt
     1561 cggctacgct ctccatttcg gttctgatag cagcgtattc gtcggtgagg tcggagtggc
     1621 tgccctttgt atcgacaatg caaagtgagt ggccgcactt ggcaaagtca aagtcaacct
     1681 cttctatcac gggatttttc gggtcgttga aatctattgt tacgaagccg ccgacggagc
     1741 acgccatctg gtccataagt ccgcagggct tgccgaaata ttcgttctcg gcatactgtg
     1801 ctattttggc tattgtgacc gcgtcaacct tgccgtcgtt gtaaagatag tttaccatag
     1861 tgccgacaag aacttcatat gcggcagatg acgagagtcc cgagcccgca ataacccttg
     1921 atgcggtagt ggcgtcaaag ccgcctatct tatagccgta tttcttaaag cctgcaagaa
     1981 caccgcgcac gagagcgggt gaatgtccca tttcgttggg gtttaccgag aggtccgcag
     2041 cgtcaacaac gtccatggaa tagccctcgg atttaactct tacaatgttt tcgtcgtttt
     2101 ttgaagccgc ggctattgcg tcaaggttga ttcccgccgc aagcactctg ccgtggttgt
     2161 ggtcggtgtg gtttccgccg acttcggttc tgccgggagc ggagaaaaga cgaacatctc
     2221 tgttctttcc gaaaagagcc tcaaattcgt ttaaaagatt tatgtacctg tcatactgct
     2281 ctttaacatt ttcctcggtg acataggctt ttttaagcct ttcatcgaga gatgcagaga
     2341 gaatttcttt tttaagggtt tcgggagttg ccattttcat ttttcatcct ttcctgccga
     2401 aaaagtttcg gcagtctttt tgtttttatt attagctact atatctataa atttattaac
     2461 cgcgagtatg gaaacaccgg agatggtgaa gataatcaca atcggcagca taaacgcgag
     2521 tgaaaacggg ttataaacgt gacggcctat catgctgtaa gaaataattt taggcgtgaa
     2581 tcccaaaagt gacagcaaaa taaacttcca atagtcgtat tcaagtgccc cgtagagctg
     2641 acttatggaa tttatcggga agtacggcac tagcctgaaa agcaccaaaa tccaatcggt
     2701 gcttctttta cccgattcca tcatacggcg aacattatcg tatcttgtga gaatcttgtg
     2761 tattgttccg ccgccgaggt gcttgcccca ataaaacttt atcgtcataa gcaatgtcat
     2821 gccggcaaaa ttcacagcca cggctatcgg cgtggggaat accataccgg ctataacaca
     2881 gaccgcggtt atcggcaggg gaataagcac tttgcctatg tatataagga aaatcacgag
     2941 aatcacgagc cacttgctcg gaattgacgc aacggcctcc tcaaactccg caagacgctc
     3001 gagcacttcg tcatatgccg acgaaacatc gtttatctgc ataagaaaag tcaacactaa
     3061 aagtgcgact gccaaaatca gcagcacaca tgctaaaata ttcaggagct ttcttcgcgt
     3121 aagttctctg tcggtcaaat cggtaccacc caaaaattat tattgcaggg catagtaata
     3181 gattatatat aattttatat catattattg gtaaggtcaa acaaaatatg aaaatatgaa
     3241 gtaatttttg ccttttaggt gtaaattcct tttaggttgt gttacaatta actcaaaagc
     3301 aaagctttac cgaaaggcgg ttttttatat gaagacagtt attcttgacg gcaagacgac
     3361 aaatccgggc gatttgagct gggattttct gaaaaaatac ggcgaggtcg agattttcga
     3421 ccgcacaccg cgcgaaaaaa tcgttgaacg aacacacgac gcagacatag tcgtaacaaa
     3481 taaaactccg ctcacaagag ccgagataga caaaatgcaa aacgtgaaat tcgtcgcgct
     3541 cctgtcgacg ggctacaacg tggtggactg cgcgtatctc aaggaaaaag gcataccggt
     3601 ttcaaatata ccggcttatt ccaccatggg cgtggcgcag ctcgtatttg catttatttc
     3661 ggagcttgcc gtaggcgttg ccatgcattc tatcgccgta aagagcggcg aatggtcaaa
     3721 ttgccccgac ttttgctttt gggaaaagcc gcttaccgag ctttccggca aaacgatagg
     3781 catagtcggc ttcggaaaaa tcggcggagc cgtagccgac atagccgagg cttataaaat
     3841 gaacatcata gcgctgtcgg gtcacgttac cgaccagagc gcacgcaaga atttcagatg
     3901 ggcaaagaac ctcgatgatc ttgcggaaaa cgccgacatt attacgttcc attgtcctct
     3961 cacaaaagag acggaaaaaa tcgtaaacgc cgattttctg agcaagtgca aaaaaacagc
     4021 gtttatcata aacacctcac gcggaccggt tgttgacgaa aaagctcttg ccgacgcgct
     4081 gaattcggga aaaatcgcag gcgcgggagt ggacgtgctg tcaaccgagc cgccggcaaa
     4141 agacaatccg ttgctcacgg cgaaaaactg ctttatcacg ccgcacatag cgtgggcggc
     4201 atttgaaacg cgcgagagac ttatgggaat attcaaaagc aacatcgagg cgtttgtttc
     4261 gggcaagccg ataaatgttg taaatccgta aaagcaaaat gtgtttgccg tttgaaaatt
     4321 cggccgatgc catacaaaaa gagctgtaaa ttaaatttac agctctttgt tttttattcg
     4381 gttaagggag tgcgtttatc aataagcgaa attggtgtaa gcaccctcaa cgtgcattgc
     4441 gcccttaccg ttaacgctga tgaacgctac cttgatattg agctcagcta cgttgccgtc
     4501 ataggaatac ttaaattcct taaggttgcc gtcctcgata actacggttg ccttgatgtt
     4561 ggtgtattca attgttgcgg agtttatctt tgccgcgctt ataaaattct tgatttcgct
     4621 gtcaaccatg ctctgagtga gaatgtcgtt tgtaagtctt gacatagcgg tcgatttatc
     4681 cgtt
//
LOCUS       DBMDVO010000021         4616 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7839_length_4616_cov_1.234994, whole genome shotgun sequence.
ACCESSION   DBMDVO010000021 DBMDVO010000000
VERSION     DBMDVO010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7839_length_4616_cov_1.234994"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            217..360
                     /locus_tag="ACZPE2_00605"
     CDS             217..360
                     /locus_tag="ACZPE2_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318377.1"
                     /translation="MAFVTSEITATPPYALTATKLSRTQRGAKLQASGSRVSEFQGTA
                     GMS"
     gene            370..1623
                     /locus_tag="ACZPE2_00610"
     CDS             370..1623
                     /locus_tag="ACZPE2_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019142.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HIR1318378.1"
                     /translation="MMITEIKGYVKVSRKAFTKVTALIVALAIMCAVTAFAASPSFYV
                     DIYDGNEITRVLTSKSDPKTIVSQAGVKLDDRDKLDSTGFTAGEDSALRIYRSATVSF
                     TDIEGNTIYTVFAGTVQDLLEELDVTLSDGQLITYPLDTILKDGMDVRVTNSYDVSVT
                     ADGATNSFTMGEGTVRDALNRIGVTLGDDDEVSPELDSEVCEGTAITVYRVSYSYRTV
                     TETVEFTKKTEKSAELYTDQQVISQKGVNGSKKVTYCDKTVDGKYASSEAVTTVVLEQ
                     AVSQITTVGTKQRPIVVRNLKNNGSPISELTVPSSINIENGAPTSYSKIITGKASAYT
                     ASPTAKTSTGRTVKAGYVAVNPKQIPYGSELWIVSTDGIVYGYAIAADTGGFIHKGKF
                     TVDLFMNTESECRQWGARDVIIYVL"
     gene            complement(1675..2412)
                     /locus_tag="ACZPE2_00615"
     CDS             complement(1675..2412)
                     /locus_tag="ACZPE2_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318379.1"
                     /translation="MKADFYDEIRRLNKSCAGKADIALTREGFHTVFSLLQRESVAEN
                     DDRYIFALFSLRNFLRKAYLSPDRPKRRTRINVGAFINALTVSCSFLFGDSDKKAFAV
                     SLPTDVSAETDDVLFSAAAAEIIGNSLLYSKRCHTLVSGGVSGDMLFISVESFGDFTA
                     YDFCRETKNDGGLSFCLGVARLLKGTLLFECGSGSADFCRKVTLGVGANEIKHESEAL
                     FPAETKRLVCDFLDDGLSVPRIFMPPI"
     gene            2659..3687
                     /gene="gap"
                     /locus_tag="ACZPE2_00620"
     CDS             2659..3687
                     /gene="gap"
                     /locus_tag="ACZPE2_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1318380.1"
                     /translation="MSVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTDPKMLAHL
                     LKYDTAQGGYCGKIGEKAHTVEAGEDSITVDGKKITIYAMKDAKDLPWGEIGVDVVLE
                     CTGFYTSKDKAQAHIDAGAKKVVISAPAGNDLKTIVYSVNEKTLDKEDKIISAASCTT
                     NCLAPMAKALNDYAPIQSGIMSTIHAYTGDQMILDGPHRKGDLRRARAGAANIVPNST
                     GAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILTAVVKGADVTKEGINAAMKAAASE
                     SFGYNEDPIVSSDVIGMRYGSLFDSTQTMVSHIADDLYEVQVVSWYDNENSYTSQMVR
                     TIKYFAEL"
     assembly_gap    3894..3903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3904..4390
                     /locus_tag="ACZPE2_00625"
     CDS             <3904..4390
                     /locus_tag="ACZPE2_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318381.1"
                     /translation="AYVVDGTTISVPDEYVTALENIFSTVDLTEDQYNQIKGILDEAL
                     EYCKANNLVKLSDITEKKATNALLAYANKALGVVGYSVTASGSLADADHGLLTILDSE
                     GKVVAKLHPAVTYSKGGQGGTIAKTGADFTSAAFCGLAAVAVLSAAGVAVKKFRKDSD
                     D"
BASE COUNT         1244 a         1111 c         1105 g         1146 t
ORIGIN      
        1 gtcgcgcagt accataagca atgtaattgt tacaaaattg taataaattg ttacgatttt
       61 taacttttac acaaatattt taataaattt gtcagtaatt attctcgata agaggtgtat
      121 tcgcgactat ttgacataat ctgacaaaca acattgtgca aaatagataa aaatcgagaa
      181 tgaaaatatt tgacaattat gtgacctgga aatataatgg ctttcgtcac aagcgaaata
      241 acagcgacgc cgccgtatgc acttacggca acaaagcttt cgcgaacgca acgcggggca
      301 aaattacagg cgtccggcag ccgcgtttcc gaatttcagg gaacggcagg tatgagttaa
      361 aggagcagaa tgatgataac agaaattaag ggctatgtaa aagtgagccg caaggctttt
      421 acaaaggtca cggcacttat agtagcgctc gcgattatgt gcgccgtaac cgcttttgca
      481 gcctcgccgt cgttttatgt tgacatatat gacggcaacg aaataacgag agtcctcacg
      541 tcgaagagcg accccaagac catagtgtcg caggcgggcg ttaaacttga cgacagagat
      601 aagctcgatt ccaccggctt cacggcaggc gaggacagcg ctctgaggat ttaccgctcg
      661 gcaacagtca gcttcaccga tattgaaggc aatacgatat acaccgtatt tgcaggtact
      721 gtgcaggacc ttttggagga gcttgacgta acgctttcgg acggtcagct tataacctat
      781 cctctcgaca ccatactcaa ggacggtatg gacgtcagag tgacaaattc ctacgacgtg
      841 tcggtcaccg cggacggcgc cacaaactcg ttcaccatgg gcgaaggtac cgtgcgtgac
      901 gctctcaaca gaataggcgt tacactcggc gatgacgacg aggtgtcgcc cgagcttgac
      961 agcgaggtct gcgagggcac ggctataacc gtttacaggg tttcgtattc ttacagaacc
     1021 gtcaccgaaa cggttgaatt tacaaagaaa acagaaaaga gcgcggagct ttacaccgac
     1081 cagcaggtta tttcccaaaa gggtgtgaac ggctccaaaa aggtaacgta ctgcgacaag
     1141 accgtggacg gcaaatatgc gtcgagcgag gcggttacaa ctgtggttct tgagcaggcg
     1201 gtttcgcaga taacgaccgt gggcacaaag cagcgcccga ttgttgtaag aaatcttaaa
     1261 aataacggct cgcccatttc cgagcttacc gttccgtcaa gcataaacat tgagaacggt
     1321 gctccgacga gctattcgaa aataattacc ggtaaggcgt ccgcctatac cgcgtcccct
     1381 acggcaaaaa cctcaacggg cagaaccgtt aaggcgggtt acgttgccgt aaatcccaaa
     1441 cagataccct atggtagcga gctttggata gtctccaccg acggtattgt ttacggatac
     1501 gccatagctg ccgatacggg cggatttatc cacaagggta aatttaccgt ggacctgttt
     1561 atgaatacag aatccgaatg caggcagtgg ggcgcaagag acgtaataat ctacgtttta
     1621 taatccccga atacttgtga ctgagagccg taaagctgat tttacggctc ttttttatat
     1681 gggcggcata aaaatacgcg gcacggaaag cccgtcgtcc aaaaaatcgc agacaaggcg
     1741 ttttgtctcc gcgggaaaga gcgcctcgct ttcatgttta atttcgtttg cgccaacgcc
     1801 gagtgtcacc ttgcggcaga aatcggcact tcccgagccg cactcaaaaa gcagtgtgcc
     1861 ctttagtagc cttgcgactc cgagacaaaa ggacagaccg ccgtcatttt ttgtttcgcg
     1921 gcagaaatca tatgcggtaa aatcgccgaa gctctcaacg gagataaaca gcatatcccc
     1981 ggaaacgccg ccgctgacga gcgtgtggca ccgttttgag taaagcaggg aattccctat
     2041 aatttccgcg gcagccgcgg aaaacagaac gtcgtccgtc tcggcggaaa cgtccgtcgg
     2101 gagcgaaacc gcaaacgcct ttttatcgct gtcgccgaac aaaaacgagc agcttaccgt
     2161 aagagcgttt ataaatgctc cgacatttat gcgcgttcgc cttttgggcc tgtcgggcga
     2221 gagataggct tttctcaaaa aattgcgcag cgaaaaaagc gcgaaaatat atctgtcgtc
     2281 gttttcggcg accgactctc tttggagcag ggaaaagacc gtgtgaaatc cctcgcgtgt
     2341 gagcgcgatg tccgctttgc cggcgcagct tttgtttaaa cgccttatct catcataaaa
     2401 atcagctttc atcctatccc ccgaagacaa taaatactgc tttataagta ttgctttacc
     2461 gcgaaaaaat atacccgaat ttcgcggccg aggacacttc tcaactaaca ggaaacttcc
     2521 agcgacacac tttttatgca gtttgacaaa acattcacag aacaggcctt tttcgcataa
     2581 aaaatcttgc ataattgcgt atttcggtat agaataatag agcaataatt taaaaacaat
     2641 ttattggagg taataccaat gtcagttaaa gttgctatta acggtttcgg tcgtatcggc
     2701 cgtcttgcgt tcagacagat gttcggcgca gagggttatg aggtagttgc catcaacgat
     2761 cttaccgatc ccaagatgct cgctcacctt ctcaagtacg ataccgctca gggcggttac
     2821 tgcggcaaga tcggtgaaaa agctcacacc gttgaggccg gcgaagactc catcaccgta
     2881 gacggcaaaa agattactat ctatgctatg aaagacgcta aggatcttcc ttggggcgag
     2941 atcggcgttg acgtagttct tgagtgcacg ggcttctata cttctaagga taaggctcag
     3001 gctcatattg acgcaggcgc taagaaggtc gttatttccg ctccggcagg caatgacctc
     3061 aagacaatcg tttacagcgt taacgaaaag actcttgaca aagaggacaa aatcatttcc
     3121 gctgcttcct gcaccacaaa ctgccttgct cccatggcta aggctcttaa cgactatgct
     3181 cccattcaga gcggtatcat gtcgacaatc catgcttaca ccggcgatca gatgatcctc
     3241 gacggtcctc atcgcaaggg cgaccttcgc cgtgctcgtg caggtgccgc aaacatcgtt
     3301 ccgaactcaa cgggtgctgc taaggctatc ggccttgtta tccccgagct caacggcaag
     3361 cttatcggct ccgctcagcg tgttccggtt ccgaccggtt ctaccaccat ccttacagct
     3421 gttgttaagg gtgccgacgt taccaaagag ggcatcaatg ccgctatgaa ggctgctgct
     3481 tccgagagct tcggctacaa tgaggatccg atcgtttcct ccgatgttat cggtatgaga
     3541 tacggctcac tctttgattc gactcagacc atggtttctc acatcgctga cgacctttat
     3601 gaggtacagg ttgtttcttg gtatgataac gagaacagct acacaagcca gatggtaaga
     3661 accattaagt atttcgctga gctgtaatca agcgatattg cagtatttaa agagctcgac
     3721 ggctgaggcc ggcgggctct ttttttaaaa aggtgtttac aaaagacttt ttgtgtgcta
     3781 caataactat aattctttac tggatattat agctatcact gtccggtaat gaaaaatttt
     3841 ttcaaaggga gagttcctac atgaagaaaa tggctagcct cttggttgta gcgnnnnnnn
     3901 nnncgcatac gttgttgacg gcactacaat cagtgttccc gatgagtatg taactgctct
     3961 tgagaacatt ttctctaccg ttgatctcac tgaagatcag tacaaccaga tcaagggcat
     4021 tctcgacgaa gctcttgaat actgcaaagc caacaacctt gttaagcttt cagacatcac
     4081 cgagaagaaa gcaacaaacg cccttctcgc ttatgctaac aaagctctcg gcgttgtagg
     4141 ttacagcgta accgcttcgg gttcgcttgc agatgctgat cacggccttc tcacaatcct
     4201 cgattcagag ggtaaggtag ttgctaagct ccatccggct gttacttaca gcaagggcgg
     4261 tcagggcggc actatcgcta agaccggtgc cgacttcact tcagctgcat tctgcggtct
     4321 tgctgcagta gctgttcttt cggctgcagg cgtagcagtt aagaagttcc gcaaggattc
     4381 agatgactga ttccggtact aatcggagta agaagagcta tcggaagagt tttataattc
     4441 ttccgatagt ttttttactt ataggctatt tgctgttttt tatatgcctc actccaatac
     4501 tcagccccct tgtctcgatt tacgagctcg cattttccaa tgcgaacgtc aacaccattg
     4561 tcgagcccgg ggaaaacagt gcttttaatg gtggcagcgg tgtggataac ggttat
//
LOCUS       DBMDVO010000022         4587 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7914_length_4587_cov_1.412171, whole genome shotgun sequence.
ACCESSION   DBMDVO010000022 DBMDVO010000000
VERSION     DBMDVO010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7914_length_4587_cov_1.412171"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(69..572)
                     /gene="rpsE"
                     /locus_tag="ACZPE2_00630"
     CDS             complement(69..572)
                     /gene="rpsE"
                     /locus_tag="ACZPE2_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1318382.1"
                     /translation="MAYKNDSAEQEFQERVVTINRVSKTVKGGRIYKFSALVVVGDGK
                     GRVGFGIGKSGEVPDAIRKGIEDAKKNIINVALKGTTIPHEITGKFGAGVVLLKPAAP
                     GTGVIAGGPVRAVVETVGIRDIRSKALRSNNPCNVVRATIDGLSKLRNADEVAAVRGK
                     TVKEILD"
     gene            complement(590..949)
                     /gene="rplR"
                     /locus_tag="ACZPE2_00635"
     CDS             complement(590..949)
                     /gene="rplR"
                     /locus_tag="ACZPE2_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1318383.1"
                     /translation="MVTRPDSKKARLKRHTRVRSKISGTAECPRLDVFRSLQNIYAQL
                     IDDVNGVTLVSASTTEKDFKDYGGNIDAAKAVGKKLAERAAEKNIKDVVFDRGGYVYH
                     GRVAALAEGAREGGLNF"
     gene            complement(965..1510)
                     /gene="rplF"
                     /locus_tag="ACZPE2_00640"
     CDS             complement(965..1510)
                     /gene="rplF"
                     /locus_tag="ACZPE2_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1318384.1"
                     /translation="MSRIGKHPIAIPAGVDVTIDGSTVTVKGPKGTLTRTVHSNMNVA
                     MENGEIVVTRPDESNLNKSLHGLTRTLIHNMVVGVTDGFKKELEINGVGYRAAKQGSD
                     LVMNIGYSHQVIVPEVDGITIEVPAPNKIVISGPDKQQVGQFAAEVREKRPPEPYKGK
                     GIKYADEHIRRKEGKAGKGGK"
     gene            complement(1523..1921)
                     /gene="rpsH"
                     /locus_tag="ACZPE2_00645"
     CDS             complement(1523..1921)
                     /gene="rpsH"
                     /locus_tag="ACZPE2_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1318385.1"
                     /translation="MQITDSIGDMLTRIRNANSAKHETVSVPASNMKKAIAQILVDEG
                     YIKSFKVVEDGKQGIIEMDLKYGPNKSSVITGIRRVSKPGLRIYTNCEDMPKVMKGLG
                     VAILSTSKGVMTDRAARKANIGGEVLAFIW"
     gene            complement(1952..2137)
                     /locus_tag="ACZPE2_00650"
     CDS             complement(1952..2137)
                     /locus_tag="ACZPE2_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR1318386.1"
                     /translation="MAKKSMKVKQASPAKYSTRQYNRCKICGRPHAYLRKYGVCRICF
                     RELAHKGQIPGVKKASW"
     gene            complement(2151..2693)
                     /gene="rplE"
                     /locus_tag="ACZPE2_00655"
     CDS             complement(2151..2693)
                     /gene="rplE"
                     /locus_tag="ACZPE2_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1318387.1"
                     /translation="MAARLKEMYASEVAPALQKKFGYKSVMQIPKFDKIVINVGCGEA
                     KDNSKVVDAIMTDLATITGQKPVECKAKKSVANFKLREGMTIGVKVTLRGDRMFEFMD
                     RLFNLALPRVRDFRGINGNSFDGRGNYAMGIKEQLIFPEIEYDKIDAVRGMDICFVTT
                     AETDEEAKELLTLMGAPFAK"
     gene            complement(2711..3043)
                     /gene="rplX"
                     /locus_tag="ACZPE2_00660"
     CDS             complement(2711..3043)
                     /gene="rplX"
                     /locus_tag="ACZPE2_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1318388.1"
                     /translation="MNKVHVKTGDTVVVINGKNRGRKGKVMQVSPAEDKVIVEGVNVV
                     TKHVKPKKMGEQGGLVKAESALYASKVQLVCPKCGAATRVGHTKVENKNGKTVSMRVC
                     KKCGAQFE"
     gene            complement(3060..3428)
                     /gene="rplN"
                     /locus_tag="ACZPE2_00665"
     CDS             complement(3060..3428)
                     /gene="rplN"
                     /locus_tag="ACZPE2_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1318389.1"
                     /translation="MIQQQTYLKVADNTGAKEIMCIRVLGGSGRRYANIGDVIVASVK
                     KAAPGGVVKKGDVVKAVVVRTVSGVRRDDGTYIRFDENAAVLIKEDKTPRGTRIFGPV
                     ARELRDKDYMKILSLAPEVL"
     gene            complement(3450..3707)
                     /gene="rpsQ"
                     /locus_tag="ACZPE2_00670"
     CDS             complement(3450..3707)
                     /gene="rpsQ"
                     /locus_tag="ACZPE2_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1318390.1"
                     /translation="MSERNLRKTQVGIVTSDKMQKTVVVSIKDRVKHPLYNKIINRTV
                     KIKAHDENNECGIGDRVLVMETRPLSKDKRWRVVEIIEKAK"
     gene            complement(3728..3931)
                     /gene="rpmC"
                     /locus_tag="ACZPE2_00675"
     CDS             complement(3728..3931)
                     /gene="rpmC"
                     /locus_tag="ACZPE2_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1318391.1"
                     /translation="MKAKEIKALSPSELDAKLTELKDELFKLRFQQAINQLDNPMRIS
                     AVKKDIARIKTVMRDVELHGAQG"
     gene            complement(3931..4362)
                     /gene="rplP"
                     /locus_tag="ACZPE2_00680"
     CDS             complement(3931..4362)
                     /gene="rplP"
                     /locus_tag="ACZPE2_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1318392.1"
                     /translation="MLLPKRVKYRRVQRGRLTGKALRGNKVTYGDFGLVATEPAWITA
                     NQIEAARIAMTRYIKRGGQVWIKIFPDKPITEKPAETRMGSGKGSPEYWVAVVKPGRV
                     LFEIAGVPEEAAREAMRLAANKLPIKCKFVAKADQSEDGEQ"
     gene            complement(4362..>4587)
                     /gene="rpsC"
                     /locus_tag="ACZPE2_00685"
     CDS             complement(4362..>4587)
                     /gene="rpsC"
                     /locus_tag="ACZPE2_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537246.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1318393.1"
                     /translation="KIQVSGRLGGAEIARTEQYHEGTIPLQTMRADIDYGFAEAKTTY
                     GRVGVKVWLYKGEVLHDNRRPRKEKEGGTK"
BASE COUNT         1001 a         1191 c         1010 g         1385 t
ORIGIN      
        1 ggtctttttt gcttccgata aggctcttaa cgagctttac ttctaaattt gccattgtaa
       61 gccctccctt aatccaatat ttctttaact gttttgccac gtacggcagc aacttcatca
      121 gcattgcgaa gctttgaaag tccgtcgata gtagctctta ctacgttgca ggggttgtta
      181 gaacgaagag ccttcgatct gatgtctctg atgccgactg tttcaacaac ggcacgtacg
      241 ggaccgcctg cgataacgcc ggtaccggga gcagcgggtt taagaagaac tacgcctgcg
      301 ccgaatttac cggtgatctc atggggaatt gtcgttcctt tgagagcaac attgataatg
      361 ttctttttag cgtcctcgat acccttgcgg atagcatccg gaacctcgcc ggatttgcct
      421 ataccgaagc cgactctgcc tttaccgtcg ccaacaacca caagagcgga gaacttgtat
      481 atgcgtccgc ccttaacggt cttggataca cggttgatag taacgactct ctcctgaaat
      541 tcctgctctg cggaatcgtt tttataagcc atttctttgt tctcctttct tagaagttga
      601 ggccgccctc acgggcgcct tcggcgagag cagcaactct gccgtgatat acgtaaccac
      661 cgcggtcaaa aacaacgtct ttgatgttct tttctgcggc tctttctgcc aactttttgc
      721 cgactgcttt cgcagcgtca atgtttccgc cgtaatcctt aaaatccttc tcggtcgttg
      781 acgcagaaac gagtgtaaca ccgtttacgt cgtcaatgag ctgagcgtag atgttctgaa
      841 gtgaacggaa aacatcaagg cgaggacact cagctgttcc ggagatcttt gagcggactc
      901 ttgtgtggcg tttaagtctt gcctttttgc tgtcaggcct tgtaaccatt taaaacactc
      961 ctttttattt tccgccttta ccggctttac cttctttgcg gcggatatgc tcgtcagcgt
     1021 atttgatgcc cttgccctta taaggctccg gcggacgctt ttcgcgaact tcggctgcga
     1081 actgacctac ctgctgcttg tcgggaccgg aaattacgat tttattgggt gccggtacct
     1141 ctattgtgat accgtcaacc tcgggaacga ttacctgatg tgaataaccg atattcataa
     1201 caaggtcgct tccctgctta gctgcacggt aacctacacc gtttatctca agctccttct
     1261 tgaagccgtc ggtaacgccg acaaccatat tgtggataag agtacgggta agaccgtgaa
     1321 gtgatttatt aaggtttgac tcgtcgggac gagtaactac gatttcgccg ttttccatgg
     1381 cgacgttcat attgctgtgt acggttctgg taagagtacc cttggggccc ttaacggtaa
     1441 cagtactgcc gtcaatagta acgtcaacgc cagcgggaat ggcaatcgga tgtttgccga
     1501 ttcttgacat actgaaacct ccttaccaga tgaatgcgag aacctcgccg ccaatattgg
     1561 ccttgcgagc tgctctgtca gtcataacgc cctttgaagt tgaaaggata gcaacgccga
     1621 ggcccttcat gaccttaggc atatcctcac agtttgtgta tatacgcaga ccgggctttg
     1681 atactctgcg aataccggtg atgacagacg atttgttggg accgtatttg agatccatct
     1741 caatgatgcc ctgcttgccg tcttcaacaa ccttaaagct ctttatatag ccctcatcaa
     1801 caagaatctg agcaatcgcc ttcttcatgt tagatgcggg aacgctcacc gtttcatgct
     1861 tagcagaatt cgcgttgcgg attcttgtaa gcatatcacc gatggaatca gtaatttgca
     1921 tactgtttac ctcctttgca attgctcgta attaccagct ggccttcttg acgccgggta
     1981 tctgaccctt gtgagctaac tcacggaagc agatacggca tactccgtac ttgcggagat
     2041 aagcatgcgg cctaccgcag attttacatc tgttgtactg tcttgttgaa tacttagcag
     2101 gagaagcctg cttaaccttc attgatttct ttgccactgt ttatccctcc ttatttggcg
     2161 aacggagcgc ccatgagcgt aagcagctct ttggcttctt cgtcggtttc ggcagtcgtt
     2221 acgaagcaaa tgtccatacc gcgaactgcg tctatcttat cgtactcgat ttcggggaat
     2281 atgagctgct ctttaatacc catagcatag ttgcctctgc cgtcgaaaga gttgccgttg
     2341 attcctctga agtcacgaac tctcggaaga gcgagattga agagtctgtc cataaactca
     2401 aacattctgt cgcctctgag cgtaacctta acgccaatcg tcatgccctc acggagcttg
     2461 aagtttgcga ctgacttttt agccttgcac tcaaccggct tctgaccggt gatggtagcg
     2521 aggtctgtca ttatagcgtc aacaaccttt gagttgtctt tagcttcgcc gcagcctacg
     2581 ttgataacga tcttgtcaaa tttcgggatc tgcataacgc tcttgtagcc gaacttcttc
     2641 tgaagcgccg gagcaacttc cgaagcgtac atttctttaa gtcttgctgc catgttgtta
     2701 tgtccctccc ttattcaaac tgtgcgccgc attttttgca tacgcgcatc gaaacagtct
     2761 tgccgttttt gttttcaacc ttggtgtggc ctactcttgt agcagcgccg cacttggggc
     2821 aaacgagctg aacctttgaa gcgtaaagcg cgctctcggc cttaacaagg ccgccctgct
     2881 cgcccatctt tttaggctta acgtgctttg tgactacgtt tacgccctca acgattacct
     2941 tatcctcggc gggagagacc tgcatgacct ttccttttct gccgcggttt ttaccgttga
     3001 tgactacgac ggtatcacct gttttaacgt gaactttatt catctcattg cacctcccat
     3061 taaagcactt cgggagcaag tgacaagatc ttcatgtaat ccttatcacg aagctctctt
     3121 gctaccggcc cgaaaatacg agtgcctctc ggggttttgt cttcttttat caataccgct
     3181 gcattctcat cgaaacggat ataagttccg tcgtctctgc gaacgccgct tactgttctt
     3241 acaacgaccg cttttacaac atcgcccttt ttaacaacgc cgccgggtgc tgcctttttg
     3301 accgaagcta caataacgtc gccgatattt gcataccttc tgcctgaacc accgagtacg
     3361 cgaatgcaca taatttcttt cgcaccggtg ttgtcggcta ctttgaggta agtctgttgc
     3421 tgtatcacag ttatagcctc cttagaaaat tatttcgctt tctcgatgat ctcgactaca
     3481 cgccatctct tatcctttga gaggggacgt gtttccataa caagaactct gtcgcctatt
     3541 ccgcactcat tgttctcgtc atgagccttg attttaactg tacggttaat gatcttattg
     3601 taaagcggat gcttaactct gtccttgatt gaaacaacga cggtcttctg catcttatcg
     3661 cttgttacga taccaacctg agtttttctg aggtttcttt cactcatttt aaaagtcctc
     3721 cctttcctta gccttgagcg ccgtgtaatt caacatcgcg cataacggtt ttgattcttg
     3781 cgatgtcttt tttaacggca cttatgcgca ttggattatc gagttggttg atggcctgct
     3841 ggaagcgtaa tttgaaaagc tcatccttaa gctctgtgag tttagcatcc aactcggagg
     3901 gggagagtgc ttttatttct tttgctttca ttactgctca ccatcctctg actgatctgc
     3961 ctttgcgaca aatttgcatt taatcggaag cttgtttgct gcaagacgca tagcctcacg
     4021 agctgcttcc tcgggaacac cggcgatttc gaaaagaact ctgccgggct taactactgc
     4081 tacccaatat tcgggagaac ctttaccgga acccattcgg gtctcagccg gtttttctgt
     4141 aatcggctta tccgggaaga tctttatcca aacctggccg ccacgcttaa tgtatcttgt
     4201 catagcaata cgggctgcct ctatctggtt tgcggttatc catgcgggct cggttgctac
     4261 aaggccgaag tcaccgtaag ttactttatt tcctcttaaa gccttaccgg taagacgtcc
     4321 tctttgaact ctgcggtatt taactctctt aggtaataac attatttagt gcctccttcc
     4381 ttttccttgc gaggcctgcg gttgtcgtga aggacttcac ctttgtaaag ccaaaccttt
     4441 acgccgacac gaccgtaagt cgttttagcc tctgcgaagc cgtaatcaat gtcggcacgc
     4501 atggtctgaa gcggaatggt accctcgtgg tactgctcgg tacgtgcgat ttcagcacct
     4561 ccgagacggc cgcttacctg aatctta
//
LOCUS       DBMDVO010000023         4571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_7957_length_4571_cov_1.172036, whole genome shotgun sequence.
ACCESSION   DBMDVO010000023 DBMDVO010000000
VERSION     DBMDVO010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_4571_cov_1.172036"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..1409)
                     /gene="tilS"
                     /locus_tag="ACZPE2_00690"
     CDS             complement(63..1409)
                     /gene="tilS"
                     /locus_tag="ACZPE2_00690"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1318394.1"
                     /translation="MNRDVLSATKKYDMLSYGDKIVVGLSGGADSVCLTHALVSLRDS
                     LSLEVEAVHVNHGIRGEEALRDEKFCSDFCKSLGIKLTVFRFDIPLECKKTGESEEEC
                     GRRKRYECFKNTAGENAKIATAHNLNDSAETVLLNIVRGTGCKGLCGIPPIRGNIIRP
                     LIMTSRDDIELYCKENSLDFVTDSTNLQNEYKRNVIRNVVFPTLQKMNPSVLSAFSRL
                     TENATDDEKLIEEIAELKYSECKTSLGLDEEKLSGYPKALRRRILMKFLSENIKKDIS
                     AVHIYDAENTVGTAKSITLPGKIILAHKDGSLVLSEKTEEYLPFSVTFNVKDGIVEYP
                     YGKLKIQIFTQKDLQNLNKVLLDNAVDCDKIGNVLSVRSRKEGDKFSSARRKNTKTLK
                     KLFNEAKIPQPKRVCIPVVSSGEKTVWVEGFGTSSEFRVDKCTRKFIIITGLTGEN"
     gene            complement(1399..2763)
                     /gene="dnaB"
                     /locus_tag="ACZPE2_00695"
     CDS             complement(1399..2763)
                     /gene="dnaB"
                     /locus_tag="ACZPE2_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1318395.1"
                     /translation="MDNLTFDNLTEPYSLESEQAVLGSILIDPQCLSDVMTVLKAEHF
                     YIPQHRDIYSVIVTLDALGSKIDPLIVLEELKKNEVYNDADGKNYLAQLAQAVPSTAN
                     VMNYAKIVKEKFTLRELMNVSHDVLHQCSEQSENAETILDSAEQRIYNIRQGKTTDGP
                     TKLGDIVINDVIPNVTLLSTPEGKDRKGIPTGFSVLDKYMTGLNKSDLILIGARPAMG
                     KTSFALNIARNAAVTSRKKVLFFSLEMSKEQLAQRILATEARIESSKMRTGDISPEEW
                     VRIATCSDALSKAELYFDDTSNITVPEMKARVRRLKDVDLVVIDYLQLMTGTKRTDNR
                     AQEVAEITRSLKLMAKDLKIPVVTCSQLNRQSASGKGKDHKPTMSELRESGSIEQDAD
                     IILLLYRESYFDEDKGESVVADENKAQVIIAKNRHGGTTDVDLNWNGQYTLFTTAEYN
                     RNEP"
     gene            complement(2786..3226)
                     /gene="rplI"
                     /locus_tag="ACZPE2_00700"
     CDS             complement(2786..3226)
                     /gene="rplI"
                     /locus_tag="ACZPE2_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1318396.1"
                     /translation="MEVILKKDVKGLGKAGEKVKASDGYARNFLFPKGLAVEANAQTL
                     TEFRNSESSKQHKIEVEIDNANKAKAKLDGKTVKLKAKAGENGKLFGSVTVKEAADAV
                     GKQLGVTVDKRKMSMADIKNFGTYTAEVKLYNGITAKLYVEVGE"
     gene            complement(3275..>4571)
                     /locus_tag="ACZPE2_00705"
     CDS             complement(3275..>4571)
                     /locus_tag="ACZPE2_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1318397.1"
                     /translation="GVFRKLGTGRFIAIVPESELEKMISKKFNVLENVRSYTYNGTTV
                     GITLSAGVGRGDNLSDAEGAARQALDMALGRGGDQVAVKDNDKYTFFGGVSKGVEKRT
                     KVRTRVVASAMSELIRNASNVVIMGHKFSDLDSVGAAYGMYKAALALGKDAKIVVNRK
                     TTLAQPLIDYIGKSDDDCFVSPLTGERLTVKKTLLIVVDTHKADFVDSKNVYEKAQNV
                     IVIDHHRKTVDYINNAVIFYHEPHASSTCEMVTELLQYISKHPVADSVCATALMSGIM
                     LDTKEFVLRAGVRTFEAAAYLRSRGADTVTVKSFFANSMESRKLRGMVVMNAEMYRAC
                     AISVTDIESDDIRVVCAQAADELLSVSGVDASFVIFKTDDIINISARSMGKVNVQLIM
                     EALGGGGHQTMAAVQLKDITESQIKDMLKTAIDNYYSNL"
BASE COUNT         1154 a         1107 c          915 g         1395 t
ORIGIN      
        1 ttcctttcat ttttcttcgg acaagagaac gctctcaatg tcattcttat agtctgcctt
       61 cattaattct cccccgttaa acctgttata attataaatt ttcttgtaca tttatcgacg
      121 cgaaattcag atgatgtacc gaagccctca acccaaacgg ttttttctcc gctgctgaca
      181 accggtatac aaactctctt cggctgcgga attttcgctt cgttaaacag ctttttaagc
      241 gtctttgtat tctttctgcg cgcagaagag aatttatccc cttcttttct gcttcgcacc
      301 gaaagaacat tgcctatttt atcacaatcc acggcattat ccaatagcac tttgttaaga
      361 ttttgtaaat ctttttgagt gaaaatctga attttaagct taccgtacgg atattccacg
      421 atgccgtctt ttacattgaa cgttacggaa aaaggtaaat actcctctgt tttttcggaa
      481 aggacgagcg aaccgtcctt gtgagcgagt attattttac ccggcaaggt tatgctttta
      541 gccgtaccca ccgtattttc ggcatcatat atgtgaactg cggatatatc ttttttaata
      601 ttttcgctta aaaacttcat taagatcctg cggcgcaatg ccttgggata acccgaaagc
      661 ttctcttcgt ccaagccgag cgaagtttta cattcggagt atttaagctc tgcgatttct
      721 tctatcagtt tttcgtcatc cgtcgcattt tcagtaagtc gggaaaatgc cgaaagaacg
      781 gacggattca ttttttgcag agtcggaaaa actacgtttc tgataacatt tctcttatat
      841 tcgttttgaa gatttgtgct gtcggtaaca aaatcaagag aattttcttt acagtacaat
      901 tcaatgtcgt cgcgcgaagt cataatgagc ggtcttatta tattgccgcg tatcggcggt
      961 ataccgcaaa gtcccttgca gcccgttccg cgtacaatgt tcaaaagcac ggtttcggcg
     1021 ctgtcattaa gattgtgtgc cgttgcgatt tttgcgtttt cgcctgccgt gtttttaaag
     1081 cactcatacc tttttcgcct gccgcactcc tcttcgctct cgcccgtctt tttacactca
     1141 agcggtatgt caaagcggaa tacggtcaat tttattccca ggcttttgca aaaatcggaa
     1201 caaaattttt catctcgcaa agcctcttcg ccgcgtatac cgtggtttac gtgaacagct
     1261 tcgacttcaa gcgaaagcga atctctgagt gaaacaagag catgtgtaag acacaccgaa
     1321 tccgctccgc cggaaagccc cacaacgatt ttatcgccgt aagacagcat atcatatttt
     1381 tttgttgccg ataatacgtc acggttcatt tctgttatac tccgcggtcg taaacaaggt
     1441 atactgaccg ttccagttaa ggtctacgtc ggtagtgccg ccgtgacggt ttttggctat
     1501 tatgacctgc gccttattct cgtctgcaac aacgctttcg cccttgtcct catcaaaata
     1561 cgattcacgg tacaagagta aaattatatc ggcgtcctgc tctattgaac ccgattcacg
     1621 aagctccgac atagtgggct tgtgatcctt gcccttgccc gacgccgact gacggttaag
     1681 ctgagagcag gtaaccaccg gtattttaag gtcttttgcc ataagcttta agcttcttgt
     1741 aatttcagct acctcctgcg cacggttatc ggtacgcttc gttccggtca taagctgaag
     1801 gtagtcaata actacaaggt caacatcttt aaggcggcgc actcttgctt tcatttcggg
     1861 aacggttata tttgacgtat cgtcaaaata tagctccgcc tttgaaagcg cgtcggaaca
     1921 cgtggcaatt ctgacccact cttcgggaga aatgtcgccc gttctcattt ttgagctttc
     1981 aattctcgct tcggtggcaa ggatacgctg agcaagctgc tcttttgaca tctcaagtga
     2041 aaagaaaaga acctttttac gcgaagtaac cgcggcattg cgtgcaatat tcagcgcgaa
     2101 gcttgtttta cccattgcag gacgagctcc gataagaata aggtcggact tgttaagtcc
     2161 tgtcatatat ttatcaagca cggaaaaccc cgtaggaata ccctttcggt ccttgccctc
     2221 gggagttgaa agcaatgtca cgttggggat aacgtcattt atcactatat cgccgagctt
     2281 agtcggtccg tcggttgttt tgccctgacg aatgttatat atgcgctgct ccgccgaatc
     2341 gagtatggtt tcggcatttt ccgattgttc cgaacactgg tgaagtacat cgtgcgaaac
     2401 attcataagc tcacgaagag tgaacttttc cttaactatt ttggcatagt tcataacgtt
     2461 tgccgttgac ggtactgcct gagcaagctg tgccaggtaa tttttgccgt cggcatcgtt
     2521 ataaacttcg ttctttttga gctcctcaag aactatcaac gggtctattt ttgaaccgag
     2581 agcgtcaagc gttacaataa ccgaatagat atcgcggtgc tgcggaatat aaaaatgctc
     2641 agccttaagt accgtcatta catccgaaag acattgcggg tcaatgagta tagaacccaa
     2701 aacagcctgt tccgattcaa gcgaatacgg ctctgttaaa ttgtcaaaag tcaggttgtc
     2761 catttatttc acatccggtt atgttttatt ctccgacttc aacgtaaagc tttgcggtaa
     2821 ttccgttgta aagcttcacc tctgccgtat atgtgccgaa atttttgatg tcagccatag
     2881 acatcttgcg cttgtcaacg gtaacgccga gctgtttgcc gactgcatct gcagcttctt
     2941 taaccgtaac cgaaccaaaa agcttgccgt tttcaccggc cttagcctta agttttacag
     3001 tcttgccatc gagctttgcc tttgctttat ttgcattatc tatttcgacc tctatcttat
     3061 gctgcttgga ggattcgctg ttgcgaaact ccgtgagagt ctgagcattt gcctcaactg
     3121 caagtccctt aggaaaaaga aaattacggg cataaccatc ggacgccttg actttttcgc
     3181 cggctttgcc gagtcccttt acatcttttt taagaatgac ctccatattt atcaatcctt
     3241 tcttttatac ggcacccgtt tttcggaacc gaaatcataa attactgtaa taattatcaa
     3301 tggcggtttt aagcatatct tttatttgac tctcggtaat atccttgagt tgaaccgccg
     3361 ccatggtctg atgtcctccg ccaccgagcg cctccattat aagctggacg tttaccttgc
     3421 ccatggaacg cgccgatata tttatgatat catcagtctt aaatataaca aatgacgcat
     3481 cgacaccgct tacagaaaga agctcatccg cagcttgtgc acaaaccaca cgtatatcgt
     3541 cagattcaat atcggtaacc gaaatcgcac aagcacgata catttctgca ttcataacaa
     3601 ccataccgcg aagctttctt gactccatgg aatttgcaaa aaatgatttt accgttacgg
     3661 tgtccgctcc acgggagcga agatatgctg ccgcctcgaa agtacgtact ccggctctta
     3721 aaacaaattc ttttgtatca agcataatac ccgacattaa ggccgtagcg caaaccgagt
     3781 ctgcaacagg atgcttgcta atatactgca aaagctccgt taccatttca cacgttgaag
     3841 aagcatgcgg ctcgtgataa aaaataacag cattatttat gtaatcaact gttttcctgt
     3901 ggtgatctat aactattaca ttttgagctt tttcatatac atttttcgag tcaacgaaat
     3961 ccgccttgtg agtgtcgact acaataagta aggtcttttt gaccgtgaga cgctcacccg
     4021 ttaaaggaga aacgaaacaa tcatcgtcac tttttcctat atagtcaata agaggttggg
     4081 caagcgttgt ttttctgttt accactattt tcgcgtcttt accgagcgcc aaagccgcct
     4141 tgtacatacc atatgcggca ccgacgctgt caagatcgga gaatttgtga cccattataa
     4201 ctacattaga cgcatttctt ataagttccg acatagcgga agccacgacc cttgtgcgaa
     4261 cctttgtacg cttttcaacg ccctttgaaa cgcctccgaa gaacgtgtac ttatcgttat
     4321 cctttacggc aacctgatcg ccgccgcgac cgagagccat atcgagagcc tgacgtgcgg
     4381 caccttcggc gtcgcttaaa ttatctcccc tgccgacgcc cgcggaaagc gttattccga
     4441 cagtagtacc gttataagta tagcttctaa cgttttcaag cacattgaat ttttttgaaa
     4501 tcatcttttc aagctcggat tcgggtacta ttgctataaa tcgccctgtt ccgagtttgc
     4561 ggaaaacgcc g
//
LOCUS       DBMDVO010000024         4509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8105_length_4509_cov_1.299682, whole genome shotgun sequence.
ACCESSION   DBMDVO010000024 DBMDVO010000000
VERSION     DBMDVO010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8105_length_4509_cov_1.299682"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="ACZPE2_00710"
     CDS             <1..471
                     /locus_tag="ACZPE2_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014668.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1318398.1"
                     /translation="ADLTLSVNSRRAFLKGAELLSRRAHGKAELTRKLMQKYPRECAE
                     QAADKLEKLGLINDGEFADMLAQELYTHRKYGVSRVKQELLRRGIDRALAEEAAERLD
                     KDDLNRIILLLQTKYRNNLGDEKGIIRTKNSLLRLGYSYSDIRAAFESIENESF"
     gene            502..1830
                     /gene="rimO"
                     /locus_tag="ACZPE2_00715"
     CDS             502..1830
                     /gene="rimO"
                     /locus_tag="ACZPE2_00715"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1318399.1"
                     /translation="MSKKVGMISLGCPKNQVDGEIMLSKLADNGFEITNDVDGADVVI
                     VNTCGFIEDAKKEAIENILDMVELKKEGVIRKIVVTGCLAERYRDEVLSEIPEVDAVV
                     GIGSNSDICEVCEKVCGGEEDIKTFGEKTCLPLDGKRMLTTPSYYAYIKIGEGCSNNC
                     TYCAIPSIRGKYRSRTPESILEEAKTLVDGGVKELIVVAQDTTRYGEDLFGKCALPAL
                     LTSLSKIEGLEWIRVLYLYPERLSDEIIDTFANNDKIVNYMDIPIQHCNSDILRRMNR
                     RGSKEELTALITKIREKIPDVVLRTTLITGFPGETEEQFSELSEFVKEMKFDRLGCFA
                     YSQEEGTPAAEFPDQVDEEIRKKRGEIIMEQQYNIFEKLNHDNIGKTMRCVVEGYDGY
                     TDSYYGRTWRDAPDIDGSVSFTCGYELNEGDFVDVEIFDVNEYDLIGEVI"
     gene            1827..2429
                     /gene="pgsA"
                     /locus_tag="ACZPE2_00720"
     CDS             1827..2429
                     /gene="pgsA"
                     /locus_tag="ACZPE2_00720"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1318400.1"
                     /translation="MKMNLPNKLTIARIIITPFFLAALLWEALPHRFLIATAIYAVGA
                     ITDAVDGHLARKNGQVTNFGKLLDPIADKILTTSALLAFMTMGLCNIWIVMIVLAREF
                     AVASVRMIAAADGVVIPANKWGKAKTVSQMTFTIIIMLLCEADYIAVTSFSKELFAAA
                     NTWFTLPHISNLLLWITAALTVVSGAIYLKDSKNVIDYSK"
     misc_binding    2473..2671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2719..3399
                     /gene="cysE"
                     /locus_tag="ACZPE2_00725"
     CDS             2719..3399
                     /gene="cysE"
                     /locus_tag="ACZPE2_00725"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR1318401.1"
                     /translation="MFDRLKEDIQSVKDRDPAATSTIQILLLYPGLKAIRMHRRAYWC
                     WNHNMKFLGRYISQRAAKKTGIEIHPAAKIGRRFFIDHGTGVVIGETTEIGDDVTLYQ
                     GVTLGGTGKDTGKRHPTIGNNVLIGAGAKVLGPFKVGDNTNIAAGAVVLEEIPPDCTA
                     VGVPARVVKHKGVRVDCLDQIHIPDPVSQELCRMSHRIALLEKELKKYQKTEYEEQNE
                     RNYEDENI"
     gene            3386..>4509
                     /gene="cysS"
                     /locus_tag="ACZPE2_00730"
     CDS             3386..>4509
                     /gene="cysS"
                     /locus_tag="ACZPE2_00730"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1318402.1"
                     /translation="MKIYNTMSRKKEELKTITPGEVKIYACGPTVYNYIHIGNARPLC
                     VFDTFRRYLEYRGYKVRFVQNFTDIDDKIIKKAIEEGSDYKTVSEKYIKEYWTDTKGM
                     NVREATVHPRATENIDEIIDIISTLVEKGYAYPVSNGDVYFSPSKFKEYGKLSHQPLE
                     DLEAGARINVEELKREPMDFALWKGAKPGEPYWESPWGNGRPGWHIECSAMVRRFLGK
                     TIDIHCGGQDLIFPHHENEIAQSECCNGVPFAHYWMHNGYINVDNVKMSKSLGNFFTV
                     RDVAEKYGYEPIRYLMISSQYRSPINYSVDIIEQCKASLQRLYTCRDSLDFALQNAED
                     ALPDNAEEIKKSLLSHKERFIEAMDDDLNTADGLSAVFELV"
BASE COUNT         1316 a          960 c         1161 g         1072 t
ORIGIN      
        1 gcggacttaa ctttgtccgt gaactcccgt cgcgcttttt taaagggtgc cgagcttctg
       61 tcgcgtcggg cgcacggcaa ggcggagctt acgagaaagc ttatgcagaa gtacccgaga
      121 gagtgcgccg agcaggcagc ggataagctc gaaaagctcg gacttataaa tgacggcgaa
      181 tttgccgata tgctggcgca ggagctttat acacaccgca aatacggcgt cagccgtgtc
      241 aaacaggagc ttttgaggcg cggaattgac cgggctttag ccgaagaagc ggctgaaaga
      301 cttgacaaag acgatttaaa tcgtattata ttattgttgc aaacgaagta cagaaataat
      361 ctcggagacg aaaagggcat aattcgcaca aaaaattccc ttctgcgcct cggctattcg
      421 tattccgaca tacgtgcggc gtttgagagc atagaaaacg aatcgttttg attacagcgt
      481 gaaaggctat gtgtgagttt tatgagtaaa aaagtcggta tgatatctct cggctgtccc
      541 aagaaccagg ttgacggtga aattatgctg tctaagcttg cggacaacgg ctttgagata
      601 acaaatgacg tagacggagc cgacgtggtc atagttaata cctgcggctt tattgaggat
      661 gcaaaaaaag aggccattga aaacatactt gatatggtcg aattgaaaaa agaaggcgta
      721 atacgtaaaa tagttgtcac gggctgcctt gcggaaagat acagggacga agtattgtcg
      781 gaaattcccg aggttgacgc cgttgtcggc ataggctcga attctgacat atgcgaggta
      841 tgcgaaaagg tttgcggcgg cgaagaggat attaaaacct tcggcgaaaa aacctgcctg
      901 ccgcttgacg gtaagcgtat gctcaccaca ccgtcctatt acgcatatat aaagataggc
      961 gaggggtgct caaacaactg cacctattgt gcaatacctt caattcgcgg aaaatacaga
     1021 agccgcacgc cggagagcat tttggaagag gctaaaacgc ttgtagacgg cggagtaaaa
     1081 gaacttatag ttgttgcgca ggacacaacg cgttacggag aggacctttt cggtaaatgc
     1141 gcgctgccgg ctcttttgac atcgttatca aaaatcgagg gcctggagtg gataagagtt
     1201 ttatatcttt atcccgaaag gctcagcgac gagataatag atacatttgc gaataacgat
     1261 aaaatcgtaa attatatgga tattccgatt cagcattgca attcggatat tttaaggcgt
     1321 atgaaccgcc gcggctcaaa agaggagctt acggcactca taacgaaaat aagagagaaa
     1381 atacccgatg tggtgcttcg tactaccctg ataacaggct tcccgggcga aacggaggag
     1441 cagttctccg agctttcgga atttgtcaag gaaatgaagt ttgacaggct cggctgcttt
     1501 gcctactctc aggaggaggg aactccggcg gcggaattcc ccgaccaggt ggatgaggag
     1561 atcaggaaaa agcgcggcga aatcataatg gagcagcagt acaatatatt tgaaaagctg
     1621 aatcacgaca acatcggcaa aacaatgcgc tgtgttgttg agggctatga cggctatacc
     1681 gacagctatt acggcagaac ttggcgtgac gctccggata tagacggctc tgtaagcttt
     1741 acctgcggtt atgaactcaa tgagggcgac tttgtggatg tggagatatt cgacgtaaat
     1801 gaatatgatc tgatcggaga agtcatatga agatgaattt gcccaataaa ttgaccattg
     1861 ccagaataat aataacgccg tttttccttg cggcattgct ttgggaagca cttccgcacc
     1921 gttttcttat cgcaacggcg atttacgccg tcggagcgat aacggacgcc gttgacggcc
     1981 atcttgcgag aaaaaacggt caggtgacga atttcggaaa gctgcttgac ccgatagcgg
     2041 ataagattct cacaacctcc gctctgctcg cgtttatgac tatggggctt tgcaatattt
     2101 ggattgttat gatagtgctt gcaagagaat ttgccgtggc ttcggtcaga atgatagcgg
     2161 ccgcagacgg agtcgtaatt cccgccaaca agtggggaaa ggccaaaact gtcagtcaaa
     2221 tgacctttac gattataata atgcttcttt gcgaggcgga ctatatagcc gtaacgtcgt
     2281 tttcaaaaga gctttttgcg gcggcaaata cttggttcac attgccgcat atttcaaatt
     2341 tactgctgtg gataaccgcc gctctgacag tagtttcggg tgctatttac ttaaaggaca
     2401 gtaaaaatgt tatcgattat tcaaaataat tggtgcaaaa cacgaaaaat gtgatacaat
     2461 atattaagat gtaaaaatgc attgatcgga aagagtagcg atcttacgcg attttcagag
     2521 agagggcacc tgtgctgaaa tgcccttaag gctgcgtgtc gtgaagtgcg tccgtgagca
     2581 ggcgaaggga aaaagagtat cttcgcacgt tttccgcgtt aaggataatg agttataaaa
     2641 cgaagtggaa ccgcgtactc acgccttcgc aggatgattt ttctgcggag gctttatttt
     2701 tttgcgggag gtgtttaaat gtttgaccgc ttaaaggaag atatacagtc ggttaaggac
     2761 cgcgacccgg cggcaacctc gaccatacaa atattgcttt tatacccggg gcttaaggcc
     2821 atcagaatgc atagaagagc ttactggtgt tggaaccaca atatgaaatt tctcggcaga
     2881 tatatttctc agcgagccgc caaaaagaca gggatagaga ttcatcccgc ggctaaaatc
     2941 ggcagacgct tttttattga ccacggcacg ggagttgtaa taggagaaac cacggagata
     3001 ggcgatgatg taacgctcta tcagggagta acgctcggcg gtaccggcaa ggataccggt
     3061 aaaagacatc caacaatagg aaataacgtg ctgataggcg ccggcgcaaa ggtgctcggc
     3121 ccttttaagg taggcgataa tacaaatata gcggcaggtg ccgtagtgct tgaggaaata
     3181 ccgccggatt gcacagccgt cggagttccg gcaagagtcg taaagcataa gggcgtgcgt
     3241 gtcgattgcc ttgaccagat tcacattccc gaccccgtat ctcaggagct ttgcagaatg
     3301 tcgcaccgca tagcgctgct tgaaaaagag ctgaaaaaat atcaaaaaac cgaatacgaa
     3361 gaacaaaacg aaaggaatta cgaggatgaa aatatataac acgatgtcac gaaaaaaaga
     3421 ggagcttaaa accataactc cgggcgaagt caaaatctat gcctgcggtc ccaccgttta
     3481 taactatatc catatcggca acgcgcgtcc tctttgcgtt ttcgatacgt tccgccgtta
     3541 ccttgaatac cgcggctaca aggtgagatt tgttcagaat tttaccgata ttgacgataa
     3601 aataatcaaa aaagccatcg aagagggctc cgattacaaa accgtgtcgg aaaagtatat
     3661 caaagaatat tggaccgaca caaagggtat gaatgtgcgt gaggcgaccg ttcatccgag
     3721 agccaccgaa aatatcgacg agataatcga tatcatttcg actcttgtcg aaaagggcta
     3781 tgcatatccc gtttcaaatg gcgatgtgta cttcagcccg tccaaattta aggaatacgg
     3841 taagctttct catcagccgc ttgaggacct tgaggccggc gcgagaataa atgtcgagga
     3901 gcttaaaaga gagcccatgg actttgcact ttggaagggc gcaaagccgg gcgagccgta
     3961 ttgggagtca ccttggggta acggcagacc gggctggcat attgagtgct cggcaatggt
     4021 aagacgtttt ctcggcaaga ccatcgatat tcattgcggc gggcaggacc tcatattccc
     4081 gcaccatgag aacgaaatag cacagagcga gtgctgcaac ggcgttccct ttgcacatta
     4141 ctggatgcac aacggatata taaatgttga caacgtaaag atgtcgaagt cactcggtaa
     4201 tttctttacc gttcgcgatg ttgcggaaaa atacggctat gagccgatac gttatcttat
     4261 gatttcctct cagtacagaa gtcccataaa ttacagcgtt gacattatag agcagtgtaa
     4321 agcctcgctt caaaggctct atacctgccg cgactctctt gattttgctc ttcaaaacgc
     4381 agaggacgct ctccccgaca acgccgagga aatcaaaaag agccttctct ctcacaagga
     4441 acgctttata gaggctatgg acgatgacct caacaccgcc gacggacttt cagcggtatt
     4501 tgagcttgt
//
LOCUS       DBMDVO010000025         4477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8190_length_4477_cov_1.093921, whole genome shotgun sequence.
ACCESSION   DBMDVO010000025 DBMDVO010000000
VERSION     DBMDVO010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8190_length_4477_cov_1.093921"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="ACZPE2_00735"
     CDS             complement(<1..335)
                     /locus_tag="ACZPE2_00735"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR1318403.1"
                     /translation="MREIDVGEIIKAVEELCIKANKILPDDLCGCISCAEKSETDELP
                     KKIMGTLLENLDAAKELDLPICQDTGMAVIFCDVGQDVHIVGGDFENAVNEGVSRGYT
                     NGLLRKSIVR"
     gene            complement(383..832)
                     /locus_tag="ACZPE2_00740"
     CDS             complement(383..832)
                     /locus_tag="ACZPE2_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1318404.1"
                     /translation="MVEVGEILEGKVTGLTNFGAFISLPGNLTGMVHISEVSQSYVSD
                     IHEHLKENQTVKVKVIDITPEGKISLSIKKALPAQQQPQGERSFRQNKGHRNSTPNVW
                     KGQPSEKKDGPMSFEDMMDRFKKVSDEKMTDLKRATDGKRGSYSRRK"
     gene            complement(866..1189)
                     /locus_tag="ACZPE2_00745"
     CDS             complement(866..1189)
                     /locus_tag="ACZPE2_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016839.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1318405.1"
                     /translation="MAAEKRAVKAKKKKGKAKHSFILTLLLVLAVGYFTISLIDTQLK
                     IKEREKTVEQVSAQYEQQLAENERLQKVADGGSQEDYIERVAREKLGYVMPDEKVYYD
                     ITPGN"
     gene            complement(1143..1751)
                     /gene="yabQ"
                     /locus_tag="ACZPE2_00750"
     CDS             complement(1143..1751)
                     /gene="yabQ"
                     /locus_tag="ACZPE2_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1318406.1"
                     /translation="MEQGVPLMDYIVGMPRQTTNFLLSLGFGFCIGVLYDVVRTIRLI
                     FGGGKRTVLIFDILYSAFAGLVTFIFSLVITNGMVMAYVVFGEMLGFFIYYITFGVFA
                     VRFSERVTKAVKGFFIKIFKALAAPFLKLFGFFKRVFGKISQKSRKKAVKAAKKSKIH
                     LKLCGSLLYNQLNNKRNYPKMRKEVKQNGCRKKSGKSKEEKR"
     gene            complement(1730..2032)
                     /gene="yabP"
                     /locus_tag="ACZPE2_00755"
     CDS             complement(1730..2032)
                     /gene="yabP"
                     /locus_tag="ACZPE2_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR1318407.1"
                     /translation="MNGSKAAVGAAEGIKAPHNVIMEDRHTLTVSGISDVDSFDEQTV
                     IVFTDMGELTVKGEGLHINRLSLEVGEIMIEGNISSLSYSDSKPTQEGGFWSRVFR"
     gene            complement(2174..2923)
                     /locus_tag="ACZPE2_00760"
     CDS             complement(2174..2923)
                     /locus_tag="ACZPE2_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR1318408.1"
                     /translation="MKFRFFLRFAAVFSAFGAAALGAKYQKLKEQKLSLPHGFTVTAH
                     TGCDCSPDNSIESIQRAIFSDVPVIENDLTLRTDGTPVFMHADSAGDGGLLFAEAQYY
                     IASNSSTVLQNLDLKVFENLDQIQSIVKKTGLIGRCFFTGVEKDNAAKVKKYAPEIPY
                     YLNMKLNVLRLRDKEYLRRTADEIKHAGAVGINCKYTYLSKTLVEVMHEQGLSVSVWT
                     VDKKPLMLYVLSLAPDNITTRNPRLLMSLIK"
     gene            complement(2933..4309)
                     /locus_tag="ACZPE2_00765"
     CDS             complement(2933..4309)
                     /locus_tag="ACZPE2_00765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1318409.1"
                     /translation="MADSLKFYLITDLHHYAPSLGTSGKAYEKWNNKEQKCLAETGAI
                     IDAAFSKLIADKDIDIVLIAGDLTSTGAMESHLDLLPKLQRLRDAGKQVYLITATHDF
                     ISSEMEPLRCEGDELLPATPTPRPKLRELYADFGWDRAVSEHKESFSYCVKLKKGFRL
                     LCMNDDGTPERHGYTESQIEWMFSQIEEAKKNGDYIFVMNHHPCLPPNPIYPLFSKKD
                     MLADYDEITTRLADSGVNLVFTGHTHMQNIAVKRTEKGNVFYDVNTSSLVGYPTAIRK
                     VTIDGEKIDVRTEQIDNFDFDRNGLSVNDYLKNHFTFFLNDIISSTAYDIDHLADLAP
                     SFSMTAETVYKLKVPLKIIGTLLNNRTVGAAAKYLGVSGKIDDRARGIVLKDLVLQIM
                     INLYHGDEPLYPGTPEYGAMDAFMGRIKKLVRPFDKDGKIKEILDAVLSSMYDAPPED
                     WNAVLPQK"
BASE COUNT         1145 a         1133 c          901 g         1298 t
ORIGIN      
        1 cgcactattg acttgcgcaa gagcccgttt gtgtagcctc ttgaaacacc ctcgtttacg
       61 gcattttcaa aatcaccgcc gacaatatgc acgtcctgac ccacatcgca aaaaattacc
      121 gccataccgg tatcctgaca aataggcaaa tcaagttcct ttgccgcgtc gaggttttca
      181 agcaaagtac ccattatctt ttttggcagc tcatcggtct cggatttttc ggcacaggat
      241 atgcagccgc acaaatcgtc cggtaaaatt ttattggctt ttatgcaaag ctcctctacg
      301 gctttgatta tttcgccgac atctatttct ctcatattta gctccataaa aacaggcaga
      361 caaatacttg cctgccgtac atttattttc ttcttgaata cgagccgcgt tttccgtccg
      421 tggcacgctt aaggtctgtc attttttcgt cgctgacctt tttgaatctg tccatcatat
      481 cttcaaaaga cataggaccg tccttctttt ctgacggctg acccttccaa acgttgggag
      541 tgctgtttct gtgcccctta ttttggcgga aagaacgttc accctgcggc tgctgctgcg
      601 ccggcaatgc ttttttgatg gaaaggctta ttttgccctc cggcgtaatg tcaattacct
      661 ttaccttgac cgtttgattt tctttcagat gctcgtgaat atcacttaca taagactgtg
      721 aaacctccga gatgtgaacc atacccgtaa gattgcccgg caggcttata aaagctccga
      781 aatttgtgag tccggtaacc ttgccttcaa gtatttcccc tacttcgacc ataaaagaat
      841 tttatcctcc ccgtgagcca acctctcagt tgcccggtgt aatatcatag tatacctttt
      901 catcaggcat aacgtatccg agtttttcgc gtgcaacacg ttcgatataa tcttcctgac
      961 tgccgccgtc agctaccttt tgcaagcgtt cgttttcggc gagctgctgc tcatactggg
     1021 cagaaacctg ctccaccgtt ttttccctct ccttaatttt gagctgcgta tcgataagac
     1081 ttattgtaaa atacccgacc gcaagcacca aaagcaaggt aagtatgaag ctgtgcttgg
     1141 ctttaccttt tttcttcttt gcttttaccg ctcttttttc ggcagccatt ttgcttcact
     1201 tccttccgca ttttcggata attgcgctta ttatttaatt gattatacaa caaagagccg
     1261 catagtttca agtggatttt tgattttttt gccgctttta ccgctttttt tcgacttttt
     1321 tgtgatattt ttccaaaaac tcgcttaaaa aacccaaata gctttaaaaa cggagctgca
     1381 agggccttaa aaattttaat aaaaaagcct ttaacggctt tggtaacgcg ctcggaaaat
     1441 cgcaccgcaa acacgccgaa cgtaatgtaa tatataaaaa aaccgagcat ctctccgaaa
     1501 accacatacg ccataaccat accgtttgtt ataacgagcg aaaatataaa tgtcacaagg
     1561 ccggcaaaag ccgaatacaa tatgtcaaat atcagcaccg tgcgttttcc gccgccgaaa
     1621 ataaggcgta tcgttctgac aacgtcatac aatacgccta tacaaaatcc aaagccgagc
     1681 gacagcaaaa aatttgtcgt ctgccgcggc ataccgacta tgtaatccat cagcggaaca
     1741 ccctgctcca aaatccgccc tcctgcgtcg gcttgctgtc tgaatatgag agagagctga
     1801 tgtttccctc tatcataatt tccccgacct caagactcag gcggtttata tgaagaccct
     1861 cgcccttcac cgtaagctcg cccatatcgg taaagactat tacggtctgt tcgtcaaagc
     1921 tgtccacatc ggaaattccc gaaaccgtaa gtgtgtgtct gtcctccata atgacattat
     1981 gcggagcttt tatgccctcg gcggcaccta ccgcagcttt actgccgttc ataacacaac
     2041 ctcctttact ttgtacaagt atatgccgca ggttgtgttt ttatgtgcgt tacggtacag
     2101 cggacaagaa acatagcggc ttatataatc cgcgcaaaaa aacatcgccc cgaaaaacgg
     2161 agcgatgaaa aaatcactta ataagcgaca ttaaaagccg cggatttcgc gtagtgatgt
     2221 tatcgggagc gaggctcaaa acatatagca taagcggttt tttatccaca gtccaaaccg
     2281 ataccgaaag cccttgttcg tgcataacct caaccaaggt ctttgacaaa tatgtgtatt
     2341 tgcagtttat acccacggct ccggcgtgct ttatttcatc tgccgtccga cgtaaatact
     2401 ccttatcgcg aagcctcagt acatttagct tcatattgag ataatacggt atttccggcg
     2461 cgtatttttt gacctttgcc gcgttatctt tctcaactcc cgtaaagaag catctgccta
     2521 taagacctgt tttttttacg atgctttgta tttggtcaag attttcaaat acctttaaat
     2581 caagattttg caaaaccgtg ctcgaatttg acgctatgta atactgcgcc tcggcaaaga
     2641 gcaagccgcc gtctcctgcc gaatcggcgt gcataaagac cggcgttccg tcagtcctga
     2701 gcgttaagtc attctctatt acggggacgt ccgaaaaaat cgccctttgt atagactcta
     2761 tggaattgtc ggggctacag tcgcagccgg tgtgagcggt gactgtaaag ccatgcggca
     2821 aggaaagctt ttgctcttta agcttttgat attttgctcc caaggcagcc gctccgaaag
     2881 cggagaacac ggcggcaaat ctaagaaaaa acctgaattt catttaatca ccttattttt
     2941 gcggaagcac cgcattccaa tcctcgggcg gcgcgtcata cattgaggac aaaaccgcgt
     3001 caagtatttc ttttatttta ccgtccttgt caaacggcct tacaagcttt tttattctgc
     3061 ccataaaggc atccattgct ccgtactcgg gtgtccccgg ataaagcggc tcgtcaccgt
     3121 gataaagatt tatcattatc tgcaaaacaa ggtccttcag gactattccg cgcgctctgt
     3181 cgtctatttt gccggatact ccgagatatt tagccgccgc tccgacggta cgattgttta
     3241 aaagcgtacc gattatcttc agcggcacct ttaatttgta aaccgtctct gccgtcatac
     3301 tgaagctcgg tgccaagtcc gccagatggt ctatatcata agccgttgat gatattatat
     3361 cattcaggaa aaacgtaaag tgatttttca gataatcatt gaccgaaaga ccgttgcggt
     3421 caaaatcaaa attatctatc tgctctgtcc tcacatcaat cttttcgccg tctattgtta
     3481 cctttcttat cgccgtggga tagcctacaa gcgacgaggt gtttacgtca tagaaaacgt
     3541 tgcccttttc ggtacgcttt acggcgatat tctgcatatg agtgtgcccc gtgaacacga
     3601 gattcactcc cgaatcggca agacgcgtag taatttcgtc atagtctgca agcatatcct
     3661 tttttgagaa taaaggataa atcggattcg gcggcaggca cggatggtga ttcataacaa
     3721 aaatgtagtc gccgttcttc ttcgcctctt ctatctgtga gaacatccac tcaatttgac
     3781 tctcggtata gccgtgacgt tcgggtgtcc cgtcgtcatt catacagaga agcctaaatc
     3841 cttttttgag ctttacgcag taggaaaacg actccttgtg ttcggaaacg gctctgtccc
     3901 agccgaaatc cgcatacagc tcgcgaagct tcggacgcgg cgtaggcgtt gcgggtaata
     3961 gctcgtcacc ctcacaccgc aggggttcca tttctgagct tataaagtca tgtgtagccg
     4021 ttataagata cacctgtttc ccggcgtcac ggagcctttg cagcttgggc agtaaatcga
     4081 gatgactttc catagcaccc gtgcttgtaa gatcaccggc tatcaaaact atgtcaatat
     4141 ccttgtctgc aatgagcttt gaaaatgcgg catcaataat cgcccctgtc tcggcaaggc
     4201 atttttgttc cttgttgttc cacttttcat atgccttgcc gcttgttccg agcgacggag
     4261 catagtgatg aaggtcggta ataaggtaaa acttcaatga atcagccata aatatcatct
     4321 ccgttcaaaa ataatacccc ccgagagaag cacggagggc atataaaatc tttttttatt
     4381 ttgcgagagc agcaacctca agcatatatg tacctatctg gccgccgaat tcgggagcac
     4441 cgtccatgat aagatgaccc gtctttaccg cctcaag
//
LOCUS       DBMDVO010000026         4419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8345_length_4419_cov_1.325382, whole genome shotgun sequence.
ACCESSION   DBMDVO010000026 DBMDVO010000000
VERSION     DBMDVO010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_4419_cov_1.325382"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1741
                     /locus_tag="ACZPE2_00770"
     CDS             <1..>1741
                     /locus_tag="ACZPE2_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318410.1"
                     /translation="EDITASHDSFTVAENGTYNVVVTAGNGLTAEESIVINNIDKIAP
                     TVNSITVLHKGIGGVAREVSGNFFSKADTLCRFFNKTVEITVDASDEGVSGIDKIEYR
                     FVDENGQPTGSDWSVYDSENKPAKDPDFKGFVEARATDKAGNVSAILRSDGVVIDGTA
                     PTDVTVSAVYGGDKEYQEGAWSATDVNITLSSEAYSGIYKYYYRVDGGEWQELSGNTF
                     TATETGEHKYEFKAESYAALESNVTAFTVKIDRQVPVIRVSFEGTFGRWTGEGVTFNF
                     STEEASLSGINYYYDNGNGWTLIDSGTSIEITDTTNAVYRFKAVNNAGTESYPSDSYH
                     VMIDAKVPEITLTPEVTDVTPNPYKINISTEVGESGLREVTCNGQDITNEDSYTVSQN
                     GTYVFTVVGKNGRVATKIFTVTNIDGDKPVLTVSSYGKTGEWTSGDVTIVLSATSNTG
                     VTYYYDDGSGFKEMNGSVLNIAENCNKTYTFKAVNKAGVESDVSDGVVVMIDKSVPTD
                     VTVTAACGEENVASGSLVRQDVTFTLSSSASSGVKYQYSLNGGEWTNIEGNTLTTNGS
                     GAYNYKFRAVSGSG"
     assembly_gap    1742..1751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1752..1967
                     /locus_tag="ACZPE2_00775"
     CDS             <1752..1967
                     /locus_tag="ACZPE2_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR1318411.1"
                     /translation="VQVKIRLFGDVNDDGKVDSTDYNLIVYHSLGIETLTDDQLAAAD
                     LNGDGVVDLSDASVLDLVMAGKYKAGE"
     gene            complement(2050..3087)
                     /locus_tag="ACZPE2_00780"
     CDS             complement(2050..3087)
                     /locus_tag="ACZPE2_00780"
                     /inference="COORDINATES: protein motif:HMM:NF022427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HIR1318412.1"
                     /translation="MNKQTEIKESTVLLMPDGNLARPGYCKHNLFKYNPEMIKRENTM
                     RLKEWDYYQISDGNIMIQLNFFNISLATCATFGLVNLKTGRKISGMATELFTPHKNRL
                     SKNGDVPNYTEYKRGGTKLIFDVRETERRLYFEGTASGKKVKFDVTCYKLPEHESITI
                     ATPFKEQGCFFLTQKLNCLATEGTVVAGNEKYTFTKDKTFTVLDWGRGVWPHTNTWYW
                     GNGSTYLDGKLFGFELTWGFGDESNATETAIFYDGKCHKIGAVHLEKDPEDDAGWMNE
                     WHFISEDGRLDLTMKPYYDHYTNMLPLNLFGMKTHQVHGLWSGKVVLDDGTELNIKDM
                     YAFCEKVHNKF"
     gene            3321..>4419
                     /locus_tag="ACZPE2_00785"
     CDS             3321..>4419
                     /locus_tag="ACZPE2_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR1318413.1"
                     /translation="MAKNKMKKTNTIHFGIQRKIVANMTTESWANVPHVTYNYEPDVT
                     EFMIEYKRLNEDCPPEKKVTLNTLMLKIIVEGLKADPIMNSHIEFDRKLVRGEIHTFE
                     NIDISMPMVLPNGEMMTINLHNFENKNLDEMVAYIADVNRRVANTNLDEVMFDVSLDN
                     TLTALKQGKIKQTLYRLIGSKTGKHKVKTLSGKEKSNYYKIPEKDRLTKHDIEQGTIT
                     VSNIGSVYRAQRGETCLLEIVPPQVCAIAVGAVQDKPVVVVNEAGEKEIAIRQVMPLC
                     IAFDHRALDFGEIVPFIKRLDEIFAAPEIIHTWRSTGISEEHMAEIKVEREQREAKYE
                     QSKEREKARKDAEKAAEKARRDAEKARKDAEK"
BASE COUNT         1244 a          946 c         1047 g         1172 t
ORIGIN      
        1 gaggatataa ccgcttctca tgattcgttt accgttgctg agaacggaac ctacaacgta
       61 gttgtaactg cgggtaacgg ccttacggca gaagaaagca tcgttataaa caatatcgat
      121 aaaattgctc ccaccgtaaa cagcataacc gttttacata aaggcatagg cggtgtcgcg
      181 agagaggttt ccggaaactt cttcagcaag gcggacactt tatgcagatt ctttaacaag
      241 actgttgaaa ttaccgttga tgcgtcggat gagggcgtat cgggaataga caaaattgaa
      301 tatcgctttg tagatgaaaa cggtcagccg accggctccg actggagcgt ctacgacagc
      361 gaaaacaaac cggctaaaga ccccgatttc aagggctttg tagaggcaag agctaccgat
      421 aaggcaggca atgtttccgc gatacttcgt tcggacggcg tggttataga cggtacggct
      481 cccaccgatg ttaccgtttc cgctgtatac ggcggtgata aggaatatca agagggtgct
      541 tggagtgcaa cagatgtaaa tataactctt tcctccgagg cttattcggg aatttacaaa
      601 tattattacc gtgttgacgg cggcgaatgg caggagcttt cgggcaatac ctttaccgca
      661 accgaaacag gtgagcataa atacgaattt aaggccgaga gctatgcggc tcttgagagc
      721 aacgtcaccg cgtttacggt taagatagac cgtcaggtgc ctgttatccg tgtttcgttc
      781 gagggtacct tcggcagatg gacgggtgaa ggcgttacat tcaacttctc taccgaggaa
      841 gcttctcttt ccggtattaa ctattattat gacaacggaa acggctggac tcttatcgac
      901 agcggcacga gcattgagat aaccgataca accaacgccg tttacagatt taaagccgtt
      961 aacaatgccg gcaccgagag ttatccctct gacagctatc acgtaatgat agatgcaaag
     1021 gttcccgaaa taactcttac tcccgaggtt accgatgtca ctccgaatcc ttataagata
     1081 aacataagca ccgaggtagg cgagtcggga cttcgcgagg ttacctgcaa cggtcaggat
     1141 ataaccaatg aagactctta caccgtgtca cagaacggta catacgtatt taccgtagta
     1201 ggcaaaaacg gcagagttgc aacaaagata tttacggtta caaatatcga cggcgataaa
     1261 cccgtgctta ccgtatcttc ttacggtaaa acaggcgagt ggacaagcgg cgatgttaca
     1321 atagttctct cggccacaag caataccggc gttacatatt actatgacga cggatcgggc
     1381 tttaaggaga tgaacggcag cgttctcaat attgccgaaa actgcaataa gacttataca
     1441 tttaaggctg ttaataaagc gggcgttgag agcgatgtat cggacggtgt tgtcgttatg
     1501 attgataaga gcgttccgac ggacgttacg gtgacggcgg cttgcggtga agaaaatgtg
     1561 gcttccggtt cgcttgtaag gcaggatgtg acatttactc tcagctcttc ggcttcgtcg
     1621 ggcgttaaat atcagtattc gcttaacgga ggcgagtgga caaatattga gggcaacact
     1681 ctcaccacaa acggcagcgg agcatataat tacaaattcc gcgctgtcag cggctcgggt
     1741 cnnnnnnnnn ngtacaggtt aaaatacgtt tgttcggcga tgtgaacgac gacggcaagg
     1801 ttgacagtac ggattacaac cttatcgtat atcattcgct cggcattgaa actcttaccg
     1861 atgatcagct tgctgcggcg gaccttaacg gagacggtgt tgttgatctc tccgatgctt
     1921 ccgtgcttga cctcgttatg gcaggcaaat ataaagcagg cgaataaagt aaagcaagcc
     1981 caaaataaaa cgcttattcc gatttttcgg aataagcgtt ttttgtttag taagcagccg
     2041 gagcttttat cagaatttgt tgtgcacctt ttcgcagaat gcatacatat ctttaatatt
     2101 aagctccgtg ccgtcgtcga gaacgacctt gccgctccaa agtccgtgaa cctgatgcgt
     2161 tttcataccg aacaggttga gcggaagcat gtttgtatag tggtcgtagt acggcttcat
     2221 agtcaaatca agtctgccgt cctccgaaat aaaatgccat tcattcatcc atccggcatc
     2281 atcttcaggg tctttctcaa gatgcaccgc gcctatttta tggcatttac cgtcatagaa
     2341 tatagctgtt tcggttgcat ttgactcgtc gccgaagccc catgtaagct cgaagccgaa
     2401 gagcttaccg tcaagataag tgctgccgtt tccccagtac catgtattcg tgtgcggcca
     2461 cacaccgcgc ccccagtcaa gaaccgtaaa ggttttatcc tttgtaaatg tgtatttctc
     2521 gttcccggca acaacggtgc cttctgtcgc aaggcagttg agcttttgag tgaggaagaa
     2581 gcatccctgt tccttaaacg gggtcgctat tgttatgctt tcgtgctcgg gaagcttata
     2641 gcaagtaacg tcgaatttaa cctttttgcc tgaagccgtg ccttcaaaat agaggcgtct
     2701 ctccgtttcg cgaacgtcaa atataagctt tgttcctccg cgcttatatt cggtatagtt
     2761 cggaacgtcg ccgtttttgc tgagtcggtt cttgtgcggc gtgaaaagct cagttgccat
     2821 accgcttatt tttctgccgg tcttgaggtt aacaagtccg aaagtggcac aggtggcaag
     2881 cgatatatta aagaaattga gctgtatcat tatatttccg tcgcttatct ggtaataatc
     2941 ccattcctta aggcgcatgg tattttcacg ttttatcatt tcgggattat atttgaaaag
     3001 gttgtgctta caatagccgg gtcttgcaag gttcccgtcc ggcataagaa ggacggtgct
     3061 ttcttttatt tcggtctgtt tgttcatttt atccaaaatc tcctcgcttt ataaatttta
     3121 tattattata ctccaatata aatacaaatt caatctgcgt ttttttgtct gaaatacaca
     3181 aatactgctt gtgagattat agacaactgt taagctgctt gacataatat gtaaaatgtg
     3241 attttttcgc aaaaaaaagt ttatttctaa aatgatttgt attatactat ttaagtaaag
     3301 ttttgtatgg aggaatactt atggcaaaaa acaaaatgaa aaaaaccaac accatacatt
     3361 tcggtattca gcgcaagatt gtagcaaata tgacgaccga atcgtgggca aatgttcctc
     3421 acgttacata caactacgag ccggacgtta ccgaatttat gatagagtat aaaaggctta
     3481 atgaagattg tccgcccgag aaaaaggtca ctttaaatac tcttatgctc aaaataatag
     3541 ttgaggggct caaagcggat ccgattatga attctcatat cgaatttgac cgcaagcttg
     3601 tcagaggaga aatacatacc tttgaaaata ttgatatttc tatgccgatg gtcttgccga
     3661 acggcgagat gatgacaata aatcttcaca actttgaaaa caaaaacctt gatgagatgg
     3721 ttgcgtatat cgcggacgta aaccgccgtg tcgcaaacac aaatcttgac gaggttatgt
     3781 ttgatgtctc tcttgataac acgcttacag ctttaaagca gggcaaaatc aaacaaaccc
     3841 tttatcgtct tatcggctca aagaccggta agcacaaggt taagaccctc agcggaaagg
     3901 aaaagagcaa ttattataaa attccagaaa aggacaggct tacaaagcac gatatcgagc
     3961 agggtacgat aaccgtttca aatatcggct cggtttaccg tgcgcaacgc ggtgaaacct
     4021 gcttgcttga aattgttccg ccgcaggttt gtgccatagc ggtcggtgcc gttcaggata
     4081 agcccgtagt cgttgtaaat gaagcgggcg aaaaggaaat agccattcgt caggttatgc
     4141 cgctttgtat tgcttttgac cacagagcgc ttgatttcgg cgagattgtt ccgtttatca
     4201 aacgtcttga tgagatattt gcggctcccg aaataatcca tacttggcgc agcaccggta
     4261 tcagcgagga gcatatggct gaaataaagg ttgagcgtga acagcgcgaa gcaaagtacg
     4321 agcagagcaa ggagcgcgaa aaagcgcgca aggatgcaga aaaggccgcc gaaaaagccc
     4381 gcagagatgc cgagaaggca cgtaaggatg cggaaaagg
//
LOCUS       DBMDVO010000027         4412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8364_length_4412_cov_1.403387, whole genome shotgun sequence.
ACCESSION   DBMDVO010000027 DBMDVO010000000
VERSION     DBMDVO010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8364_length_4412_cov_1.403387"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /locus_tag="ACZPE2_00790"
     CDS             <1..2334
                     /locus_tag="ACZPE2_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318414.1"
                     /translation="TLGVNPTDMYVLYVIAVLANIPLTGIRANIIDNTRNKAGKYRPY
                     IVSMGIPTTIICVLMVWFPYDKFGALFGEGMLFGVTRAYFVKCTLILIFNLLLHFFYY
                     FFYDSYENLIHVLSPNSQERADVAAIKSVVYSLAPTIVNLITPIIAQNVFHTNSTDIR
                     VYRLLYPIIGIGGILLCILVYKNTQEKIVQARTHVIQIKFSDALREVAGNKYFWIISF
                     AGWIGFLEASYANVLYWLYNYGGVCSGNVYGLVVTIYGNASLWGMILAPMCIRKWGKK
                     FVLVVTNIMNIIFILMMLPFTSQMNGSTIWAIMGCLYLNAFMGSFALILNPAIQADIR
                     DYQQYKSGERIDGMFSAVATIGTVIALLTSAVLPVVYKRGGITTDNALAVTSNPDILG
                     RMLGDGKTVGEILSEQMANGQNNYSNAYSALYDPNILENLLKVLILFSALGALLNVVP
                     YFWYDFNERKQKSVVKVLKVRAMFEDYNNGAIEDKELVEAVDIIRESRALAAEKPVDV
                     SKKWYKGISDKAEKKAQKKAYKAAVQKNEDIEIAKFVCEELDKFSSNLVKYQLKTYKK
                     VYDGGLEGLRKITLDDINKELAEAKALPKTDSEEKQIRKFAISVAKKKKSAYKAIHKY
                     YGDPSVKFERLDFSVLEKYFDQEDACDDRLKTLYTELSDAKKAGNSEKVQMLRADIKK
                     TASERKWARDMSKKEMDRHAYFNRAAKPYLDAERLINQEKYYQHFGEIEALYDEAKER
                     EAEAKKARDAEVERLKAEDAAYKAQKKAEKLAKKGKK"
     gene            complement(2618..4072)
                     /locus_tag="ACZPE2_00795"
     CDS             complement(2618..4072)
                     /locus_tag="ACZPE2_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1318415.1"
                     /translation="MNYKMMGRIFAEILALEAFFMLPPLFISIYDGVDVAAKAFLYTI
                     GILIAVSAFLWLICRKAKRGFYAQEGIVCVGLGWIFLSLFGALPFFISGEIPFYIDAF
                     FEMVSGFTTTGASIVPVVEDLSRGILYWRSFSHWLGGMGVLVFLLAIVPSNSKSGGSR
                     LHLLKAESPGPDVGKLVPKMRDTAMILYVLYIALTLLNILFLLAGKMPLFDALCTAFG
                     TAGTGGFGIKNDSIAGYSIYIQWVCTVFMMLFGVNFNCYYLLIMRQFKALFKNEEVRC
                     YFGISIMSAALIAIDIRKIYPTIHETIRHACFQVASIMTTTGFATTDFDTWPSFSKTI
                     LLTLMVIGACAGSTGGGLKCARVLLLFKNLRRNIHKILHPRRVQVVHVDGTPVDEKII
                     NGTSVYLAAYFILVVLSILLISIDNKPLVTNLSAVLSCFNNIGPGLEIVGPTGNFSSY
                     SAFSKLVLIFDMLAGRLEIFPILALISRGAWKKA"
     gene            complement(4090..>4412)
                     /locus_tag="ACZPE2_00800"
     CDS             complement(4090..>4412)
                     /locus_tag="ACZPE2_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR1318416.1"
                     /translation="CPKELCCNSIVRYVRAIQNQSGAAIAVHFIADGQAQAIEFRVDE
                     QTKHCGEALKDIKIRKNVLIVCITHNGKIEIPNGNSSFKKGDGIIIVTSSGDVVRQLN
                     DIFI"
BASE COUNT         1290 a          987 c         1016 g         1119 t
ORIGIN      
        1 acactcggcg taaatccgac ggatatgtat gttctttatg ttattgcagt ccttgccaat
       61 atcccgctta cgggaatcag ggcaaatatt attgataaca caagaaataa agcgggtaag
      121 tacagaccct acatagtgtc aatgggtatt ccgaccacaa taatctgtgt gctgatggtg
      181 tggttcccgt acgataagtt cggcgctttg ttcggtgagg gaatgctttt cggcgtaaca
      241 agggcatatt ttgtaaagtg tactttgata cttattttca acctgctctt gcatttcttc
      301 tattatttct tctatgacag ctatgagaat cttatacacg ttctctcgcc gaattcacag
      361 gagagagccg atgttgcggc tataaagagc gtcgtttatt cactcgctcc gaccatagta
      421 aaccttatta caccgattat agctcagaat gttttccaca cgaattcgac cgacatccgt
      481 gtttacagac ttctttatcc gataataggt atcggcggca tacttctttg catactcgtt
      541 tataagaaca ctcaggaaaa gatagttcag gcgagaacgc acgtaattca aatcaagttc
      601 tccgacgctc tcagagaggt tgccggcaac aagtacttct ggataatctc ctttgccggc
      661 tggataggct tccttgaggc atcgtatgca aatgttctct attggcttta taactacggc
      721 ggcgtttgct ccggtaacgt atacggactt gttgttacca tttacggcaa cgcttcgctt
      781 tggggtatga tattagctcc catgtgcatc agaaaatggg gcaaaaaatt cgtgcttgtt
      841 gttacgaata ttatgaatat catattcatt cttatgatgc ttccgtttac ctctcagatg
      901 aacggctcca ccatttgggc tattatgggc tgcctttacc tcaatgcgtt tatgggttcg
      961 tttgcgctta ttttaaaccc cgcaattcag gcagacattc gtgattatca gcagtataag
     1021 agcggtgaga gaattgacgg tatgttctcg gcagttgcta caatcggtac cgttatcgca
     1081 cttcttacga gtgccgttct tcccgtagta tacaagagag gcggtatcac aaccgataac
     1141 gctctcgccg tcacatcaaa tcccgacatt ctcggcagaa tgctcggcga cggcaaaacc
     1201 gtaggtgaaa tactctccga acagatggca aacggccaga acaattacag caacgcatat
     1261 tccgcacttt acgacccgaa tattctcgaa aacttattga aggtgctcat acttttctcg
     1321 gctctcggtg ctcttctcaa cgttgtacct tatttctggt atgactttaa cgagagaaaa
     1381 cagaagtccg tggttaaggt gctcaaggta cgcgctatgt ttgaggacta caacaacggc
     1441 gccattgagg ataaagagct tgtagaggct gtcgatataa tcagagagtc aagagctctc
     1501 gccgccgaaa agcccgttga tgtcagcaaa aagtggtaca agggcatcag cgacaaggcc
     1561 gaaaagaagg ctcagaaaaa ggcttacaag gcagctgttc agaagaatga ggacattgag
     1621 attgccaaat tcgtatgcga ggagcttgat aagttctcgt ctaatcttgt taagtatcag
     1681 ctcaagacat ataaaaaggt ttacgacggc ggacttgaag gccttcgcaa aatcactctt
     1741 gacgatataa acaaggagct tgccgaggct aaggctcttc ccaagaccga cagcgaagaa
     1801 aagcagatac gcaagtttgc gatttcggtt gccaagaaga aaaagtcagc ttataaggcc
     1861 atacataagt attacggcga cccgtccgtt aagttcgagc gccttgactt ctccgtgctt
     1921 gagaagtact ttgaccaaga ggacgcttgc gacgacagac tcaagacgct ttataccgag
     1981 cttagcgacg ccaaaaaggc aggtaacagc gaaaaggttc agatgcttcg tgcagatatc
     2041 aaaaagactg caagcgagcg caagtgggca cgcgatatgt ctaagaagga aatggatagg
     2101 cacgcatact ttaaccgtgc cgcaaagccg tatcttgacg ccgaacgcct tataaatcag
     2161 gagaagtact atcagcattt cggtgagata gaggctctct atgacgaggc aaaagagcgt
     2221 gaggcagagg ctaaaaaggc aagagacgcc gaggttgaaa gacttaaggc tgaggacgcc
     2281 gcatacaagg cacagaaaaa agccgaaaag ctcgctaaaa aaggcaagaa ataataatcg
     2341 gcaaacaaaa taaaaccgaa taagcataaa gctgccctcc ttatcgcacg aagcgaccgg
     2401 ggagggcagc ttgttttatt gattttaagg ttttacaaag ccgagatata tgcatttcag
     2461 gtttgacttt aagcagggta aagaagagac aaaggttacg cgctcgtttt tatttaaatg
     2521 acaatcggct gtgcaccgat ggagagtaaa gcaaatcgca aaaaagctcc cgagagaaat
     2581 cgggagctta aaatataaat gagcggtata cggtgcgtca agcttttttc catgcgcccc
     2641 ttgaaataag agccaaaatc gggaatatct caagtctgcc ggcgagcata tcaaatatca
     2701 gcacaagctt tgaaaacgcg ctgtaagagc tgaagtttcc ggtgggtccg actatctcaa
     2761 gaccggggcc gatattgttg aagcaagaga gcacagcgga caaatttgtt acaagcggct
     2821 tgttgtcaat cgatataagc agtatactca gaacaaccaa aataaaatat gcggcgagat
     2881 aaacgcttgt gccgttgatt attttttcgt caaccggtgt gccgtctacg tgtacaacct
     2941 gaactcttct cgggtgaagt atcttgtgaa tgtttctgcg taaatttttg aaaagcaaca
     3001 gaactcttgc gcacttgaga ccgccgccgg tagagccggc gcacgcgcct atgaccataa
     3061 gtgtgagcaa aatggttttt gagaagctcg gccatgtgtc aaaatccgta gtcgcaaagc
     3121 ccgtagtcgt catgatactt gctacctgga agcaggcgtg gcgaatcgtt tcgtgaattg
     3181 tcgggtatat tttacggatg tcaatggcta tcagcgcagc actcataatt gaaattccga
     3241 agtaacagcg aacctcctca tttttaaaga gtgccttgaa ttggcgcata atgagcaagt
     3301 aatagcagtt gaagtttacg ccgaagagca tcataaacac ggtgcaaacc cactgtatat
     3361 aaatgctgta acccgctatg ctgtcatttt tgattccgaa gccgccggtt cctgcggtgc
     3421 cgaatgctgt gcacagcgcg tcaaaaagag gcatttttcc ggcgagtaaa aacaatatat
     3481 tcaaaagagt aagtgctata tacagtacat acagtatcat ggcggtatcg cgcattttgg
     3541 gtacgagctt accgacatcg gggcccggac tttccgcctt taaaaggtgc agccgcgagc
     3601 cgcccgattt tgaattagac gggacaatgg caagcaaaaa tacaagcaca cccattccgc
     3661 cgagccaatg gctgaagctt ctccaatata aaattccgcg tgataagtcc tcaacaacgg
     3721 gcactattga cgcaccggtc gttgtaaaac ccgaaaccat ctcaaaaaac gcgtcgatgt
     3781 aaaacggtat ttcgcccgat ataaaaaagg gcaatgcacc gaaaagactt aaaaaaatcc
     3841 aaccgaggcc aacgcaaact atgccctcct gagcgtaaaa gccgcgcttt gctttgcggc
     3901 agataagcca caaaaatgcg gataccgcta tcagtatgcc tattgtatat aaaaaggcct
     3961 ttgctgcaac atcgactccg tcataaatgc ttataaacag cggcggaagc ataaaaaacg
     4021 cttcgagcgc caatatttcc gcaaaaatac gacccatcat tttatagttc ataaagcact
     4081 tttaccccat taaataaata tgtcgttaag ctgtcgcacc acatctccgc tgcttgtgac
     4141 aataatgatg ccgtcacctt ttttaaacga agaattaccg ttgggtattt cgattttacc
     4201 gttatgcgtt atgcagacaa taaggacatt tttgcgaatt tttatatcct taagagcctc
     4261 tccgcaatgc tttgtctgct cgtcaactcg gaattctatg gcctgcgcct gaccgtctgc
     4321 gataaagtga acggcgattg ccgcaccgct ttgattttgt atggctctga catagcgtac
     4381 tattgagttg cagcacagct ccttgggaca ga
//
LOCUS       DBMDVO010000028         4355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8524_length_4355_cov_1.390691, whole genome shotgun sequence.
ACCESSION   DBMDVO010000028 DBMDVO010000000
VERSION     DBMDVO010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_4355_cov_1.390691"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="ACZPE2_00805"
     CDS             complement(<1..731)
                     /locus_tag="ACZPE2_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012521.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1318417.1"
                     /translation="MKAYKIHLIRHAMTEENLDGKYIGQTDVPASEYGLRQIKDIIDE
                     YGGYPEVDAVVSSPLKRCTETAKLIYPDKEPMILNGLIEYDFGEFEGRTADEMKDDDA
                     FKTWLSGEHPETPVPFGESQAAFNERVCSCFAGLIDGIIKAGVESTAVITHGGVIMSL
                     MTAFAIPSLPMHEWMAPNGTGYTLRIDPSVWSRGQKLETFSECPAVALSDDQERELWD
                     FYPTERDDEYDITEDVYGDNPDEDE"
     gene            824..1438
                     /locus_tag="ACZPE2_00810"
     CDS             824..1438
                     /locus_tag="ACZPE2_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318418.1"
                     /translation="MNSVFAARLTKLRRERGMSQKDAAKMLGVSQSLLSHYEKGIREC
                     SLDFVCRASNFFDVSCDYLLGQVDTRRSLSEEFDMTDTVQDGEYRTSTLFRASVMLND
                     SMINCGSPEKLKDYFALSIYRMAVCAANGGYIPKKWISLDCETSPVFGSVLMTDIIRE
                     LMAEANQESQKNIAEPKCVKTVVEHSEKLIRKRAAELAAEKSRG"
     gene            complement(1453..2052)
                     /locus_tag="ACZPE2_00815"
     CDS             complement(1453..2052)
                     /locus_tag="ACZPE2_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025658.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HIR1318419.1"
                     /translation="MEYWYLWLLLIVLCVITVFVLSKASAAAKKHNNERDALLREYDR
                     KKDLKDEFSFLTREKAESSDPETLLEGVSAVLQAKTEKAEDPEKVFGKFTEVQKYIYT
                     LYYFLEDTESDSLSFFFRNNGEPLTSLASKALCAVGENELSLIVEKEFSMYDENNEEV
                     SVDKDEIENLDGEFKAKLQKQQVLNSIKTYIIKNIDMLS"
     gene            complement(2055..2882)
                     /gene="rsmI"
                     /locus_tag="ACZPE2_00820"
     CDS             complement(2055..2882)
                     /gene="rsmI"
                     /locus_tag="ACZPE2_00820"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1318420.1"
                     /translation="MTGKLYVVGTPIGNLSDFSPRAAECLRNCDFIAAEDTRVTLKLL
                     NRFDIKKPLISYFEHNRRERGEIILARILGGESCALVTDAGMPAISDPGQDLVDICIA
                     SGVNVVSVPGPTAFATAVALSGLPSGRFTFEGFLSVNKQSRAEHLDSIKDEKRTMVFY
                     EAPHKLLRTLKDLYACLGDRRIAIIKELTKIHENVERTTLKKACEKYENIPIKGEFVL
                     VIEGAPQAAPEIYTVESAVAKAREFTDGGMSKNEAAKNAAKLTGIKKSDIYKILTEE"
     gene            complement(2882..3631)
                     /locus_tag="ACZPE2_00825"
     CDS             complement(2882..3631)
                     /locus_tag="ACZPE2_00825"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1318421.1"
                     /translation="MTEITLNNDEHIEYLSRDLGLIVSKEHTFGTDALLLADFASPKR
                     YDICCDFGTGCGIIPMLWCRDGCGKEISAVEIQEKACSQLTRSVKANDLQDKLFVYNA
                     DLKEAPRIFPCGKFDVITMNPPYKAENAGIKSQKENEKIARHETLCNLRDITKAASKL
                     LKFGGKLCICIRPERLFETMEAMKDYKIEPKALRLVAASPEKAPWLCLVEGRLGGKTG
                     ITVKNNLFIYKSKDVLSDEMTRILGKYREDM"
     gene            complement(3632..>4355)
                     /locus_tag="ACZPE2_00830"
     CDS             complement(3632..>4355)
                     /locus_tag="ACZPE2_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1318422.1"
                     /translation="DDEAASLIENGINPIAFPNLYVSVTSEESKAINSDPAPKVIISA
                     SGMCEAGRIRHHLKHNLWRPECTILFVGYQAVGTLGRAITDGATDVKLFDEKIHISAE
                     IEQLHGISGHADNEGLLKWMKSFKAPPKHVFVNHGEDTVCEIYAARLRDELGLSASAP
                     YNGEEYSLTENKVLVEGNREKIVKNTSSQNASRRVSTVYERLLSAQKRLQTVVAHNEG
                     GANKDLAKFADQIIALCEKWDR"
BASE COUNT          961 a         1093 c         1052 g         1249 t
ORIGIN      
        1 tcctcgtcct catcggggtt gtcgccgtac acatcctcgg ttatatcgta ttcgtcgtct
       61 cgctctgtcg ggtaaaagtc ccataactca cgctcctggt cgtcggaaag tgccacggcc
      121 gggcattccg aaaaggtttc gagcttctga ccgcggctcc acacggacgg gtctatcctg
      181 agggtatatc ccgtgccgtt cggtgccatc cactcgtgca tgggcagcga cggtatggca
      241 aatgcagtca taagcgacat aataactccg ccgtgggtta ttaccgcggt cgattctacg
      301 ccggctttta taattccgtc gataagcccg gcaaagcatg agcagactct ctcgttgaag
      361 gccgcctgac tttcgccgaa gggtacgggc gtttccgggt gctcacccga cagccaagtt
      421 ttaaaggcat cgtcatcctt catttcgtcg gcggttctgc cctcaaattc gccgaagtcg
      481 tactctataa gcccgtttaa aatcatgggc tctttgtcgg gatatatgag ctttgcggtt
      541 tccgtgcaac gctttaaggg gcttgaaacc accgcgtcaa cctcgggata gccgccgtat
      601 tcatctatga tgtctttgat ttgcctgagc ccgtattcgg atgccggcac atcggtttga
      661 ccgatatatt tgccgtcaag attttcctct gtcatggcat ggcggatcag gtgaatttta
      721 taagctttca ttttgctttg ctccttccgg gggataatac tgtacaaaca gtctaatttg
      781 atatataatt ataatacaat ttgtaatatt tgggggacat tttatgaatt ccgtatttgc
      841 cgcaaggctg acaaagcttc gcagggagcg cggtatgagc caaaaggacg ccgcaaaaat
      901 gctcggtgtg tcgcagtcgc tcctgtcgca ctatgaaaag ggtataaggg agtgcagcct
      961 tgactttgtg tgccgcgcgt cgaatttttt tgacgtgagc tgcgattacc tcttaggtca
     1021 ggtcgataca agacgctctc tgagcgagga atttgatatg accgataccg tgcaggacgg
     1081 agaataccgc acgagtacgc tttttcgcgc gtccgttatg ctcaatgaca gtatgataaa
     1141 ctgcggttcg ccggaaaagc tgaaggatta ttttgcgctt tcgatatacc gtatggctgt
     1201 ttgcgccgca aacggcggtt atattccgaa aaagtggata tcgctcgatt gcgagacaag
     1261 tccggtcttc ggcagcgtgc tgatgacgga tataataagg gagctgatgg ccgaggcaaa
     1321 tcaggaatcg caaaagaata tcgccgaacc gaaatgcgta aaaaccgttg tggagcattc
     1381 ggaaaagctg atacgcaaaa gagctgccga gttagcggcg gaaaagagcc gcggatagct
     1441 tctctccctc ttttaagaca gcatatcgat attttttata atatatgttt ttatgctgtt
     1501 taaaacttgc tgcttttgaa gcttcgcctt gaattcgccg tcaagatttt caatttcgtc
     1561 cttgtccacg gagacttcct cattgttttc atcgtacatc gaaaattcct tttcgacaat
     1621 caaagaaagc tcgttttcgc cgacggcaca gagtgccttt gaagcgagag atgtcagcgg
     1681 ctcaccgttg tttctgaaaa agaacgagag ggaatcgctc tcggtatcct ctaaaaaata
     1741 gtatagagtg taaatgtatt tttgcacctc tgtaaatttg ccgaatacct tttcagggtc
     1801 ttcggccttt tctgtttttg cctgcaagac cgccgaaacg ccctctaaca gggtttccgg
     1861 gtcggaactc tccgcttttt cacgcgttaa gaatgaaaac tcatccttta aatctttctt
     1921 tctgtcatat tctctcaaaa gagcgtcacg ctcgttgttg tgctttttag ccgccgccga
     1981 ggctttgcta agtacaaata ccgtaatgac gcacaggacg ataagaagca gccacaagta
     2041 ccaatattcc attattattc ctccgtcagg attttatata tgtcgctctt tttgattccg
     2101 gtgagctttg ccgcgttttt tgccgcctcg tttttcgaca ttccgccatc tgtgaattct
     2161 cttgccttcg cgacggcgga ctccaccgtg taaatctccg gagcggcctg cggcgctccc
     2221 tctataacta atacgaattc acccttgata ggtatgtttt catatttttc gcacgccttt
     2281 ttgagcgtgg tgcgttcaac attttcgtgg attttcgtaa gctctttaat aatcgctata
     2341 cggcggtcac cgaggcaggc ataaaggtcc ttaagcgtgc gtaaaagctt gtgcggagcc
     2401 tcgtaaaaca ccatggtgcg tttttcatcc ttaatgctgt caagatgctc ggcgcggctc
     2461 tgcttattta cgctcaaaaa gccttcaaac gtaaatctgc cggacggcag ccccgacagc
     2521 gccacggctg tggcaaatgc ggtggggccg gggacggata caacatttac tcccgatgca
     2581 atgcagatgt caacaaggtc ctgaccgggg tcggaaattg ccggcatacc tgcgtccgtg
     2641 acaagagcac agctttctcc gccgagaata cgcgcaagaa ttatttcgcc gcgctcacgg
     2701 cggttgtgct caaaatagct gatgagcggc ttttttatgt caaatctgtt taaaagcttc
     2761 agggtgactc ttgtgtcctc cgccgctata aaatcgcagt ttctgaggca ctctgcggca
     2821 cggggagaaa aatccgagag gttgcctatc ggagtgccga caacgtaaag ctttcccgtc
     2881 attacatatc ctccctgtat ttacctaaaa ttcttgtcat ttcgtcggac aaaacgtcct
     2941 tgctcttgta tataaagaga ttgtttttga ccgttatgcc cgtttttccg ccgagtctgc
     3001 cctctacaag gcacagccac ggagcctttt cgggtgaagc tgcaacaagg cgcagcgctt
     3061 tcggctcgat tttataatcc ttcattgcct ccatcgtctc aaaaaggcgc tcgggtcgta
     3121 tacaaatgca tagcttgccg ccgaatttca gaagtttcga cgcggctttt gttatgtcgc
     3181 gcagattaca cagcgtttcg tgacgggcta ttttttcgtt ttccttttgg ctttttattc
     3241 cggcgttttc cgccttatac ggtggattca tcgttatcac gtcgaatttt ccgcacggaa
     3301 atatccgcgg agcttctttc aaatcagcat tgtacacaaa gagtttgtcc tgcaaatcgt
     3361 ttgccttgac ggagcgcgta agctgactgc acgccttttc ctggatttcg actgccgata
     3421 tttccttgcc gcagccgtcc ctgcaccaga gcattggaat tatgccgcag cccgtaccga
     3481 agtcgcagca tatgtcatat cgtttcggag acgcaaagtc ggcaagcagc agcgcgtccg
     3541 tgccgaaggt gtgctccttt gagacgataa gccccaagtc cctgctgaga tattcgatat
     3601 gttcgtcgtt gttaagtgta atttcggtca tttatctgtc ccatttttcg caaagcgcaa
     3661 ttatctgatc cgcaaatttt gcaaggtcct tgtttgcacc gccctcatta tgcgccacaa
     3721 ccgtttggag acgcttttgt gccgagagca gtctctcata aaccgtgctt acgcggcgag
     3781 aggcgttttg cgaggatgta tttttaacta tcttttcgcg gttcccctca acgagaacct
     3841 tgttttcggt gaggctgtat tcctcgccgt tgtacggggc actcgcggaa agccccagtt
     3901 cgtcgcgaag acgtgcggcg tatatttcgc acaccgtgtc ctcgccgtga tttacaaata
     3961 cgtgctttgg cggagcttta aagcttttca tccactttaa caagccctcg ttgtcggcgt
     4021 gaccgcttat gccgtgaagc tgttctattt ccgcacttat atggattttt tcgtcaaaga
     4081 gctttacgtc cgtcgcaccg tcggttatgg ctctgccgag tgttcctacg gcttggtagc
     4141 cgacgaataa aatcgtacat tcgggtcgcc acaaattgtg cttcaggtga tggcggattc
     4201 tgccggcctc gcacattccg ctcgccgaaa ttatgacctt cggagcggga tctgagttta
     4261 ttgcttttga ttcctcactt gtcacggaga cataaaggtt aggaaaggct atcggattta
     4321 taccgttttc gataagcgac gccgcctcgt cgtca
//
LOCUS       DBMDVO010000029         4284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8768_length_4284_cov_1.118336, whole genome shotgun sequence.
ACCESSION   DBMDVO010000029 DBMDVO010000000
VERSION     DBMDVO010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_4284_cov_1.118336"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(51..773)
                     /locus_tag="ACZPE2_00835"
     CDS             complement(51..773)
                     /locus_tag="ACZPE2_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1318423.1"
                     /translation="MTEFQNSFKKGLKDGLPIALGYLSVSFTFGMMAVEGGLPVFTAV
                     LISMTNLTSAGQFAGLDLILAGGAYLEMALTQLVINIRYALMSVSLSQKLHKSVNLLD
                     RFLIAFSNTDEIFAVASGQKGEVGKKYMYGLALLPIVGWTLGTLIGAAASTVLPLSVR
                     SALGIAIYGMFIAIFIPPAKHNKTVAVVVLLSAALSICFKYVPGLCKVSSGFVIIICA
                     VLSATVGALLKPVKEDDKNENG"
     gene            complement(889..2775)
                     /locus_tag="ACZPE2_00840"
     CDS             complement(889..2775)
                     /locus_tag="ACZPE2_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1318424.1"
                     /translation="MSKKQKNVLWRIIVSAVLIVCLTVLGKFITLNKYIWLVLNLIPY
                     FIIGYDILKKAVKGIFKGQVFDENFLMAVATVGAIALGDYKEGVAVMLFYQIGELFQS
                     IAVGKSRRNISALMDIRPDYANVEVDGKLEKVDPDEVAVGTEITVNPGEKIPIDGVVT
                     SGDTSLNTSALTGESLPRNVKTGDEVISGCINMTGVIKVKTTKEFGDSTVSKILDLVE
                     NSSMKKSRSENFITKFARYYTPAVCFSALALAILPPVIRLIAGHTAMWSDWIFRALTF
                     LVISCPCALVISVPLSFFAGIGCASANGILVKGSNYLEGLSDTKYVVFDKTGTLTKGV
                     FEVTGVYPENGYTKDSIVRLAAYSESASSHPISLSLKKYYGKDIDENGVTDIKEIAGH
                     GVSAAVDGKRVLVGNLKLMKEEHIAVSTEHNEGTVVYVSEDGKYAGCIVISDVVKPNT
                     KLALSELKKHGVKKTVMLTGDSKAAADRVAAEIGIDEVHSELLPADKVSEVEKLLDKK
                     EPKEKLAFVGDGINDAPVLSRADIGIAMGALGSDAAIEAADIVLMDDDPEKISLAKKI
                     SVHTLKIVRENIAFALLVKAVCLVLSAVGIANMGVAIFADVGVMVVAVINATRALRLK
                     NKTE"
     gene            complement(2824..3042)
                     /locus_tag="ACZPE2_00845"
     CDS             complement(2824..3042)
                     /locus_tag="ACZPE2_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1318425.1"
                     /translation="MKKTYNIEVDCANCAAKMEEAAQKTDGVREAVVSFMTQKMKVEF
                     DDGADEAAVMKKVLKNCKRVEDDCEIYI"
     assembly_gap    3106..3115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3379..3744)
                     /locus_tag="ACZPE2_00850"
     CDS             complement(3379..3744)
                     /locus_tag="ACZPE2_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1318426.1"
                     /translation="MADNKCDCNAVMQEVVDDIKNNMFSEKEINGVSNLFKVLGDPTR
                     AKIVMALDKREVCVCDLAAALGMTKSAVSHQLALLKANNIVKARRDGKHVFYSFDDEH
                     ITTVIEIADEHIRHQHLGD"
     gene            complement(3889..3963)
                     /locus_tag="ACZPE2_00855"
     tRNA            complement(3889..3963)
                     /locus_tag="ACZPE2_00855"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3928..3930),aa:Glu,seq:ttc)
     gene            complement(3968..4044)
                     /locus_tag="ACZPE2_00860"
     tRNA            complement(3968..4044)
                     /locus_tag="ACZPE2_00860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4008..4010),aa:Met,seq:cat)
     gene            complement(4190..>4284)
                     /locus_tag="ACZPE2_00865"
     CDS             complement(4190..>4284)
                     /locus_tag="ACZPE2_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318427.1"
                     /translation="VFESHADEIFDEAENRLHAQKAVMVTLMEK"
BASE COUNT         1157 a         1022 c          853 g         1242 t
ORIGIN      
        1 ttataagata tgtgacaagc gccataaccg ctatatatat aaatatgttc tcagccgttt
       61 tcatttttgt cgtcctcctt tacgggtttt aagagggcgc cgacagtcgc ggaaagaacg
      121 gcgcaaatga ttattacaaa tcccgaagag actttacaaa gacccggcac gtatttgaaa
      181 catatgctca acgccgccga caataatacc acaacagcca cagtcttatt atgctttgcg
      241 ggcggtataa atatagctat aaacataccg taaatcgcaa tgccgagagc ggagcgcacg
      301 gacagcggaa gcacggtgct cgccgcagca cctataagag tgccgagagt ccacccgact
      361 atcggcaaaa gagcaagccc gtacatatat tttttgccga cctctccttt ttgacccgag
      421 gctacggcaa atatttcatc ggtattgctg aacgctatta aaaatcggtc aagcaaattt
      481 acgctcttgt gaagcttttg cgacagagaa accgacatca gcgcatagcg tatgtttata
      541 acaagctgtg ttagtgccat ttcaagataa gcaccgccgg cgagaattaa atcaagccct
      601 gcaaactgac ctgccgaggt caaatttgtc atagagataa gcaccgccgt aaaaacgggc
      661 aagccgccct cgacagccat cataccgaat gtaaacgaaa ccgaaagata gccgagcgct
      721 atcggcaaac cgtctttcag gcctttttta aaactgtttt gaaattccgt catttttctt
      781 cctgcgtttt cttccctacg atataaaaag cacacctcgg tgaagccccg aacggtgtgc
      841 ttttaacagc attgaatatt ataatcacaa agtcgtttta tgtcaatatt attcggtttt
      901 attctttaat cgaagtgctc ttgtggcgtt gataaccgct acaaccataa ctccgacatc
      961 ggcgaatatg gcgactccca tatttgcaat gcctactgcg ctgagcacaa ggcaaacggc
     1021 tttcacaaga agggcaaacg caatgttttc tcttactatt ttcagcgtgt gaaccgagat
     1081 ttttttcgca agcgaaatct tttccggatc gtcgtccata agcactatat cggccgcctc
     1141 tattgccgcg tccgagccga gcgcgcccat cgctatgcct atgtcggctc ttgaaagcac
     1201 gggagcatcg tttattccgt cgccgacaaa cgccagcttt tctttgggct cttttttgtc
     1261 aaggagtttt tcgacctccg ataccttatc ggcagggaga agttcagagt gaacctcgtc
     1321 tatgcctatt tccgcggcta ccctgtcggc agcggccttg gagtcgcccg tgagcattac
     1381 cgtctttttg acgccgtgct ttttgagttc gctgagagca agctttgtat tgggctttac
     1441 aacatctgat attacaatac agccggcata ttttccgtcc tcggaaacat acacaaccgt
     1501 accctcgtta tgctcggttg agacagcgat atgctcttct ttcatcagct ttaaattgcc
     1561 gactaacact cttttaccgt caactgcagc cgaaacaccg tggcctgcaa tctcttttat
     1621 atctgtaacg ccgttttcgt caatatcctt gccgtaatat tttttaagac tgaggcttat
     1681 cgggtgactc gaagcactct cggaatacgc cgccaagcgc acaatggaat cctttgtgta
     1741 tccgttttcg ggatatactc cggttacctc aaagactccc tttgtaagag ttccggtttt
     1801 atcaaacaca acatatttcg tatctgaaag cccctcaaga taattggagc cttttacaag
     1861 tataccattc gccgaagcac agcctatacc cgcaaaaaac gagagcggaa ccgatattac
     1921 aagagcgcac ggacagctga tgacaaggaa ggtaagcgct ctgaatatcc aatcgctcca
     1981 catggccgtg tgacctgcaa taagccttat aaccggaggt aaaattgcga gtgcaagtgc
     2041 cgaaaagcaa accgccggag tataatatct tgcaaatttt gttatgaaat tctcggagcg
     2101 cgactttttc atacttgaat tttcaacaag gtcaagtatt tttgatacgg ttgaatcgcc
     2161 gaactcctta gtggttttta ccttgataac gccggtcata ttgatgcagc cgcttataac
     2221 ctcatcgccc gttttgacat ttcgcggcag gctctcgccg gttaaagcac ttgtattgag
     2281 cgatgtgtca ccgcttgtaa caacgccgtc tatcgggatt ttttcaccgg gatttacggt
     2341 tatttcggta ccgaccgcca cttcgtcggg atcaaccttt tcgagttttc cgtcaacctc
     2401 gacatttgca tagtcgggtc ttatatccat taaggcggaa atattgcgtc tgctcttgcc
     2461 gacagctatg ctttggaaaa gctcgcctat ctgataaaag agcataacgg caacgccctc
     2521 tttataatcg ccgagtgcta tggcaccgac ggtagcaacc gccataagga aattctcatc
     2581 aaatacctga cccttaaaaa tgcctttaac agcctttttg agaatgtcgt agccgattat
     2641 aaaatacggt atcaggttta aaacaagcca aatgtattta ttgagagtta taaatttgcc
     2701 gaggacggta agacatacta tcagcacagc cgaaacaatt atccgccaca gaacgttttt
     2761 ctgtttttta ctcataaata tctccctcac ttatttattt gccgaaagcg gcatctcata
     2821 acatcagata tatatttcac agtcgtcctc tactcttttg caatttttga ggactttttt
     2881 cataacggct gcctcatcgg caccgtcgtc aaattctact ttcatttttt gtgtcataaa
     2941 agatacaacg gcttcacgaa cgccgtcggt cttttgagcg gcttcctcca tttttgcggc
     3001 acagtttgca caatcgactt ctatattgta tgtttttttc ataataacaa ccctttcttc
     3061 ggctgctctc tttcggaaca gttgagcaat cattcatttg ttgtcnnnnn nnnnntgtct
     3121 atattatagt tgaacatatg ctcaattgtc aatatgtttt ttaaaatttt aaattttttt
     3181 attttggtga agcatcattt ctaaagcgaa aattcgcacg gataaaagtc tttctgtctg
     3241 tacgatttca catagaaagt aaaaaaagcc ccgaataatg ttttcgcata ttcggaaccc
     3301 tgtaaattat tcaattgtga cagaatatta tatcgacaat ataatcgtcg gttttatatc
     3361 atgtatattt aagaagcctt aatcgcccaa gtgctgatgg cggatatgct cgtccgctat
     3421 ttcgataacg gttgtaatgt gctcatcgtc aaagctgtaa aacacatgct tgccgtctcg
     3481 gcgagcctta actatgttgt tcgcctttaa gagcgcaagc tgatgagaca cggctgactt
     3541 agtcataccg agtgctgcgg caaggtcgca aacgcacacc tcgcgcttat caagtgccat
     3601 aactattttg gctctcgtgg gatcaccgag gactttaaac aggtttgaaa caccgtttat
     3661 ctccttctca gaaaacatat tgttttttat atcgtcaaca acctcttgca taacggcatt
     3721 acagtcgcac ttattatcag ccattatttt tcacctcata aacatacggt atcgtactgt
     3781 taagaatatt atatatctta aaaacaaata tttcaaataa aatgtgtata cacaaaggaa
     3841 cgccaaacgg tgatgcaaaa aaggactcag cctacgctaa gtcctttttg gtgacccgga
     3901 cgagatttga actcgtgtta ccgccgtgaa agggcggtgt cttgaccgct tgaccaccgg
     3961 gccgttatgg tagcggcagt gggacttgaa cctacgacct tccgggtatg aaccggacgc
     4021 tctagccaac taagctatgc cgccatatat gccgcttttt cagcggcttt gatattataa
     4081 tataaagtgt actgcttgtc aatagtttat ttatgataaa atgaaaatta cattgcaatg
     4141 cggcataccg ttttttcaaa agccgcattg caacaaaaaa gtcaaactat tacttctcca
     4201 tgagcgttac cattaccgcc ttttgagcgt ggagacggtt ttcggcctca tcaaatatct
     4261 catccgcgtg agactcgaac acat
//
LOCUS       DBMDVO010000030         4224 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_8947_length_4224_cov_1.226777, whole genome shotgun sequence.
ACCESSION   DBMDVO010000030 DBMDVO010000000
VERSION     DBMDVO010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_4224_cov_1.226777"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            853..>4224
                     /locus_tag="ACZPE2_00870"
     CDS             853..>4224
                     /locus_tag="ACZPE2_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318428.1"
                     /translation="MKKSTKLLSVILAVVMIFSTMSVMAFAAKTEYQTSDNLTALDAY
                     SPDGAVTRLSTEERMSVVFDFLDVTLAAANINMGDVINKAGLHLVIDLRSVNALCGTI
                     DSAQALLNNGLVKLVKGLLGIVKDANLKNWPSGMTRENHAQLDIVNGIATLLKDNAGL
                     VKKVINDGKLDVGIIGNFVDISGVNKYLADLPGMLKGLVYPVFARVDDDMTLINTYST
                     TTENPDTLIKNVLINAMSKPQSYTSYKEDASGNCISNHIALPTSAEAGLRDYYVKGSD
                     SKGAYIEVFEYDTAKKTYISQDEKYYKTEETDMEGKGTGVYVYTNAAGENVKYYVKDS
                     YFLPSLATSGKVSEIFNLDSNTLVSALYQVAPYVFKDLAPVVLNGSVKMLLAQWFGAE
                     KTELFGGKASEATAVLAKLPSDVKAFYSKAAGAYNWEWSDFTIGSDGNGYYRLVSKDG
                     LTETWLKFDMSTANSFAKLINWNYTISGDFVNEFMPTAANGGSVTASAAGYTTVLAAL
                     NDFVGKAIDTMLSDTAKAAINWTKGDNTKLIPNLRKALQYVAAYNPEYLFGTGYETVY
                     AGYYDTVVDKSASDQDVVTALGAIGVKALMPQIILPSAAELKGQNVTALLACVIRELA
                     TQFVPTYNYDALIYADYNSKTLVKGKNSGYWLDVIFTMGTDIGMKYLSKLADLGSDKA
                     DGYKFAASKTYKLADFEKNTRAWEDTIDWIIDWALTSDNEWCWKVQKLINTDGLDLDL
                     ATAQDPWVKLDKIIRDVLPVEKIINETAADGKTFLETVLREDIIDRLLNLDVSKLLGT
                     NSVTGIFNIPANSTLRTEAMYPAVFRIVRELLNKVFGKVCGNTALIADSYNSLDAILQ
                     KSAIADLAEKLVVGIAYAVKSGGLLDVALPIVNFFLGWTTNAQSYAEPKLTIDKGALN
                     YVQLTNGQMNTTLKVTNASAGMLLKHGDTYDHPYMLTLKSVTVNGEEMLKNGATELSP
                     YESTDVALKATVPTDSLVKVTAVYSFTFKDGTAYDGDITSTTFEYASNDTTDVGTAWA
                     SGEQTAADLLYTYVKMKGETTTDYLVTNVSSFTSTVSNINVIWTNTRDTDCKFTKGSI
                     SGFDSSIFESSGQAESLVNQNITAEGGTATVNPIRV"
BASE COUNT         1137 a         1046 c          908 g         1133 t
ORIGIN      
        1 tcacttcgtt cgtagccagc tccctttaca agggagccta atttaatccc tcagtctact
       61 cacttcgttc gtagccagct ccctttacaa gggagcctaa tttaatccct cagtctactc
      121 acttcgttcg tagccagctc ccttttcaag ggagcctttt ttgtcgctcc gcgacatttc
      181 cccacactgt ggggaatcat ccttttcaag ggagcctttt gtcgtttccc cacaccgtgg
      241 ggaatcaccc tatttgaggg agcctaaaag ccctctgttt ttacccgaaa atcaccgtaa
      301 aaatgttcaa aaccgtgttc aatacttgtc aaaaagttca aaactcgaaa acgttaacaa
      361 tttgttcata attttacaac aacattgcca attttgtttg ccacttttgc ccaaaaataa
      421 caattctatt tgttcaatac agaaaatcaa caaatttgtg tgaacaattt attgactttt
      481 ttcgtttaaa attctatact ttaactaacc ataggaatgg tgtaataggg cgaagtgtcc
      541 ccttttttga gggagcgctg ccgcacggct gatttaccgt gcagtttcca tcccgttttg
      601 atacacatcc tacggtaata tgctcgggtg tgtatcatca gttttccacc gattcatttt
      661 tacggaagtg gaaacggtgg aaaccgaccg agttaacgtg ccgaaatcgg tttgctttct
      721 tgtaaatcaa agagcggtca gaagtacagc ggattttgtg cgaggaattg ttgcggggag
      781 tgccgcccgc agaaaaaatc gggtacgcat acatggtttt aatgtaaatc tttttaggag
      841 gaaaaaacac gtatgaaaaa atctaccaaa ttgttaagtg tcatcttggc agttgtcatg
      901 attttctcaa ctatgtccgt aatggcgttt gccgcaaaga cggagtatca gacctctgat
      961 aaccttaccg ctcttgacgc atacagcccg gacggcgcgg taacaagact ctctaccgaa
     1021 gagagaatgt cagtcgtatt tgacttcctc gatgttaccc ttgccgccgc gaacataaac
     1081 atgggcgatg ttattaacaa agcaggtttg catcttgtta tcgaccttcg ctcggttaat
     1141 gctctttgcg gaaccatcga cagcgctcag gctcttctta acaacggact tgttaagctt
     1201 gtcaaaggct tgctcggcat agttaaagac gctaatctta aaaactggcc gtcgggcatg
     1261 acaagagaaa accacgctca gctcgacata gtcaacggca ttgcaactct tcttaaagac
     1321 aacgcaggtc tcgttaaaaa ggttatcaac gacggcaaac tcgacgtcgg cattatcggc
     1381 aactttgttg atatctccgg cgttaataag tacctcgcgg atcttccggg tatgttaaaa
     1441 ggcctcgttt atccggtgtt cgcaagagtt gacgatgaca tgacgctcat caacacttat
     1501 tcaacaacaa ccgaaaatcc cgacactctt atcaagaatg tcctcatcaa cgctatgtct
     1561 aagccgcaga gctatacttc ttataaagaa gacgcttccg gcaactgcat aagcaatcac
     1621 attgctcttc cgacaagcgc tgaagcaggt cttcgcgatt attatgttaa gggctcagac
     1681 tctaagggtg cttacatcga ggtatttgaa tatgataccg ccaagaagac atatatttct
     1741 caggatgaga aatattacaa gaccgaagaa accgatatgg aaggcaaggg cacaggcgtt
     1801 tatgtttaca ctaacgctgc cggcgaaaac gtaaaatatt acgttaagga cagctacttc
     1861 cttccctcac ttgccacaag cggtaaggtt tcggaaatct tcaatcttga cagcaatact
     1921 cttgtttcgg ctctctatca ggtagctccc tatgtattta aggatcttgc tcccgttgtt
     1981 ctcaacggct ccgttaagat gcttcttgca cagtggttcg gcgctgaaaa gaccgagctc
     2041 ttcggcggca aggcttcaga ggccaccgct gttctcgcta agcttccgtc agatgtaaag
     2101 gcattctatt ctaaggctgc cggcgcatac aactgggagt ggtcggactt cacaataggc
     2161 tccgacggca acggctatta tcgccttgtc agcaaagacg gtctcacaga gacctggctc
     2221 aagtttgata tgtccaccgc taattcgttc gctaagctca ttaactggaa ctacaccatc
     2281 tccggtgatt tcgttaacga gtttatgccc acagctgcaa acggcggctc cgttactgct
     2341 tcggcagcgg gttataccac cgttcttgcg gcgctcaacg attttgtcgg caaggcaatc
     2401 gacacaatgc tttcggatac tgcaaaggct gcaatcaatt ggacaaaggg cgataataca
     2461 aagctcattc ccaatcttcg caaggctctt cagtatgtag cagcttataa ccccgagtat
     2521 ctcttcggta ccggctatga gactgtttat gcaggctact atgacacagt tgtagataag
     2581 tccgcttccg atcaggatgt tgttaccgct ctcggcgcaa tcggcgttaa ggctcttatg
     2641 cctcagataa ttctcccgtc ggctgccgag ctcaagggac agaacgtaac cgctcttctc
     2701 gcttgcgtaa ttcgtgagct tgcaactcag ttcgttccga cttacaacta tgacgcactt
     2761 atttatgccg attacaactc gaagaccctc gtaaagggta agaacagcgg ctattggctc
     2821 gatgttatct ttaccatggg caccgacatc ggtatgaagt atctctccaa gcttgccgac
     2881 ctcggctccg ataaggctga cggctacaaa tttgcagcct ccaagactta taagcttgca
     2941 gacttcgaaa agaacacacg cgcttgggaa gacaccattg actggataat cgactgggct
     3001 ctcacctctg acaacgagtg gtgctggaag gtacagaagc tcatcaacac cgacggcctt
     3061 gaccttgacc ttgctaccgc acaggatccg tgggttaagc tcgacaagat tatccgcgac
     3121 gttcttcctg ttgagaagat aatcaacgaa accgctgctg acggtaagac cttccttgag
     3181 actgttctca gagaagacat tatcgacaga cttctcaacc ttgacgtaag caagctcctc
     3241 ggtacaaaca gcgttacggg catattcaat attcctgcta acagcactct cagaaccgag
     3301 gctatgtatc ccgcagtatt cagaatcgta agagaactcc ttaacaaggt tttcggtaag
     3361 gtatgcggca acaccgctct tatcgccgat tcttacaatt cacttgacgc tattcttcag
     3421 aagagcgcta ttgcagacct cgcagagaaa ttggttgtag gtattgccta tgccgttaaa
     3481 tcaggcggtc ttcttgacgt agctcttccg atcgtaaact tcttcctcgg ctggactact
     3541 aacgctcagt cctatgctga gcctaagctt acaatagata agggtgcact taactatgta
     3601 cagcttacaa acggccagat gaacactact cttaaggtaa caaacgcatc tgcaggtatg
     3661 ctcttaaagc acggcgatac atatgatcat ccttatatgc tcactcttaa gtctgttacc
     3721 gttaacggcg aagaaatgct caaaaacggc gcaacggagc tttcaccgta tgagagcacc
     3781 gacgttgcac tcaaggctac cgttcctacc gacagccttg taaaggtaac cgctgtttac
     3841 agcttcacct tcaaggacgg tactgcttac gacggcgaca ttacttcaac tacctttgag
     3901 tatgcctcca acgatactac agatgtaggc acggcttggg cttcaggaga gcaaacagct
     3961 gcagacttgc tttatacata tgttaaaatg aagggtgaaa ccaccacgga ttatcttgta
     4021 acaaatgtat caagctttac ttctacagtt tcaaacatta acgtaatttg gaccaataca
     4081 agagatactg attgcaagtt caccaagggt tctatttccg gctttgattc gagcatcttt
     4141 gagtcatcgg gtcaagcgga atcacttgta aatcagaaca tcaccgcgga gggaggtact
     4201 gctaccgtta atcccataag agtg
//
LOCUS       DBMDVO010000031         4200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9028_length_4200_cov_1.397902, whole genome shotgun sequence.
ACCESSION   DBMDVO010000031 DBMDVO010000000
VERSION     DBMDVO010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9028_length_4200_cov_1.397902"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(51..221)
                     /locus_tag="ACZPE2_00875"
     CDS             complement(51..221)
                     /locus_tag="ACZPE2_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1318429.1"
                     /translation="MSIADIVIICILAAAVAAATVFIVRRRKKGKCIGCSGNCAACAA
                     SSSNKNADLKKL"
     gene            complement(218..2224)
                     /gene="feoB"
                     /locus_tag="ACZPE2_00880"
     CDS             complement(218..2224)
                     /gene="feoB"
                     /locus_tag="ACZPE2_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1318430.1"
                     /translation="MIFALAGNQNCGKTTLFNALTGSNQHVGNFPGVTVDQKMGSIKG
                     AKNCSVVDLPGIYSIRPYTQEEIVSRDFILDGKPDGIINIVDATNIERNLYLTLQLLE
                     LNLPMVLALNMMDEVRANGGTVNVKKLSESLGIPVIPISAAKNEGVSELADKMVYVAK
                     NRILPKRIDFCSDGPVHRCIHSVAHVIEDHARNISVPPRFCSTKLIEGDEYFADKLEL
                     DKNERELIEHSVVEMEHDTGLDRNAALADMRYTFIEKAVSESVVKCRESREHKRSVKI
                     DKILTGKYTALPVFFGVMFLVFWLTFNVIGSRLSDLLSLGIDALTDICDRGLTAYGIN
                     PIVHSLIIDGIFAGVGSVLSFLPIIVTLFFFLSILEDTGYMARVAFVMDKLLRKIGLS
                     GRSFVPMLIGFGCSVPAIMATRTLSSDRDRKMTILLTPYMSCSAKIPIYAVFTAAFFT
                     KYRALVMIGLYATGILLGIIVALILKRTAFRGEPVPFVMELPNYRMPSPKSVFLLLWE
                     KARDFLQRAFTVIFIATIIIWFLQSFDTRLNVVDDSADSLLAMIGKFIAPIFKPLGFG
                     DWRAVTALISGFTAKEAVVSTLSVLMNTGTAQLGTVLPSLFTGLSAVSFLVFTLLYTP
                     CVAAVATIKKELGSRIKTVGVVFMQCAVAYIASFIVYSVGGLFV"
     gene            complement(2257..2481)
                     /locus_tag="ACZPE2_00885"
     CDS             complement(2257..2481)
                     /locus_tag="ACZPE2_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1318431.1"
                     /translation="MTLDMLKNGETAVIKTVGGEGALRLRFLDMGLIPHTAVTLQKTA
                     PMGDPIEIRVRGYELTLRKADAKLIEVEKA"
     gene            2770..3309
                     /locus_tag="ACZPE2_00890"
     CDS             2770..3309
                     /locus_tag="ACZPE2_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HIR1318432.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTMRIREGDPLEIFTASDGEV
                     IFKKYSPIGELSEFADQYAEVLCRATDLPVIITDRDHVISVSGISKKEYLERRISSEL
                     ETLMESRTNYTASADEEPIYPISGMSREAAVAYPIIGSGDVSGCVVLLLNSDGSLPSE
                     TERKLVAVAASFLGKQMEE"
     gene            complement(3400..3675)
                     /locus_tag="ACZPE2_00895"
     CDS             complement(3400..3675)
                     /locus_tag="ACZPE2_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1318433.1"
                     /translation="MNKTELIAAVAEKTDLSKKDADAAVSAVLGAITDALKAGDKIQL
                     VGFGTFEVRNRAAKQGRNPRTGETMTVPASKVPAFKAGKALKDAVAE"
     gene            complement(3702..>4200)
                     /locus_tag="ACZPE2_00900"
     CDS             complement(3702..>4200)
                     /locus_tag="ACZPE2_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HIR1318434.1"
                     /translation="STDEVLSNWDAIKSKSKKRKTITDKMLSVPRELPALMRAQKLQE
                     KASKVGFDWPDVSGAIDKIDEESAEFKAAVKNGDSENAFEELGDLLFSAVNAARFISV
                     DAEEALTRSSDKFLSRFATVERLVKERNINMEKSSITELDKLWDEAKLNSAFCSHAEG
                     EATDK"
BASE COUNT         1107 a         1021 c          932 g         1140 t
ORIGIN      
        1 aaaaggagcc gtaaaaaccg ctgtttttac agttcctttt ttattatcgg tcaaagcttt
       61 tttaaatcag cgtttttgtt gcttgacgat gcggcgcaag cggcacaatt gccggagcag
      121 cctatacact tgcctttttt gcggcgcctg actataaaga ccgttgccgc cgccacagcc
      181 gccgccaaaa tgcagataat cacaatgtcg gcgatactca tacaaaaagg cctccgacgc
      241 tgtaaactat aaatgacgct atgtacgcaa cggcacattg cataaataca acgccgactg
      301 ttttgatgcg cgagccgagt tcctttttaa tggtggcaac agccgcaacg cacggtgtat
      361 acaaaagggt aaacacaagg aagctgacag ccgaaagccc cgtaaacagg ctcggaagaa
      421 ccgtgccgag ctgcgccgtg cccgtattca taagaacaga cagcgtactt acaacggctt
      481 cttttgcggt aaagcccgat ataagcgccg ttacggctcg ccaatcaccg aagccgagcg
      541 gcttaaatat cggtgctata aatttaccta tcatggcgag caggctgtcc gcgctgtcgt
      601 ccacaacatt gagtcttgta tcaaacgact gtaagaacca aattattatc gtcgcaataa
      661 atattacggt gaatgctctc tgcaggaaat cacgcgcttt ttcccaaagc agtaaaaaga
      721 cgcttttcgg cgatggcata cggtaattcg ggagctccat tacaaacggt acgggttcgc
      781 cgcggaaggc cgtccttttt aatatcagcg caacaattat gccgagaagt atacccgtgg
      841 cataaagtcc tatcataaca agcgcgcggt attttgtaaa aaatgccgcc gtaaacacgg
      901 catatatcgg tatttttgcg gaacagctca tatacggagt caaaagaata gtcattttgc
      961 ggtcacggtc tgacgagaga gtcctcgtcg ccattatcgc aggcacggag cagccgaagc
     1021 ctatgagcat cggaacaaag cttctgcccg aaaggcctat ttttctgaga agcttatcca
     1081 taacgaaagc gacgcgcgcc atatagcccg tatcttcgag aattgacagg aagaaaaaca
     1141 gcgtaactat tataggcaaa aagctcaaaa cgcttccgac tccggcgaat ataccgtcaa
     1201 taataaggct gtgtactatg ggatttatgc cgtatgcggt aagccccctg tcgcatatgt
     1261 ccgtcagcgc gtcaattccg agtgacagca gatccgaaag gcgcgagcct attacgttaa
     1321 aggtgagcca aaatacaagg aacattacgc cgaaaaacac cggcagcgcc gtgtattttc
     1381 ccgtgagtat tttatctatt ttcacgcttc gtttatgttc acggctttca cggcacttaa
     1441 ccaccgactc ggaaaccgcc ttttcgataa acgtatatcg catatcggca agagccgcat
     1501 tacggtcaag accggtatca tgctccattt ccacaacgct gtgctcaata agctctcgtt
     1561 cgtttttatc aagctcaagc ttgtcggcaa aatattcgtc gccctctatg agctttgtgg
     1621 aacaaaatct cggagggacc gagatgtttc gcgcatgatc ctctatcacg tgggctaccg
     1681 agtgaatgca gcggtgtacg gggccgtctg agcaaaaatc tattcttttc ggcaaaatac
     1741 gattttttgc gacatatacc attttatccg caagctccga aacaccttcg ttttttgcgg
     1801 cacttatcgg aattaccggt atgccgaggc tctcgctgag ctttttgaca ttgaccgttc
     1861 cgccgtttgc cctgacttcg tccatcatat tcagtgccag caccataggc aggttcagct
     1921 ccaaaagctg caaggtcaga tagagattcc gttcaatatt tgtggcgtca actatattga
     1981 ttattccgtc gggcttgccg tcaagtataa agtctcgcga aactatctcc tcttgtgtat
     2041 acggtcttat cgaatagata ccgggaaggt cgacaacgga gcagtttttt gcccccttta
     2101 tgcttcccat tttttggtca accgtaacgc ctggaaaatt tccgacgtgc tgatttgagc
     2161 ctgtcaacgc attaaaaagt gttgttttac cgcagttttg atttcctgca agtgcaaata
     2221 tcatttatta tcctttacct ttccgaatta aaagctttat gctttttcta cctctataag
     2281 cttcgcgtcc gccttcctga gcgtcaattc atatcccctg acccttattt ctatcgggtc
     2341 gcccatgggc gcggtctttt gaagagtgac ggccgtatgc ggaataagcc ccatatcaag
     2401 gaagcgcagt ctcagcgcac cctctccgcc aaccgttttg attacggccg tttcgccgtt
     2461 tttcaacata tcaagagtca ttttgatcct ccgcagcgtg ttagttttga ctaacttcga
     2521 cattataaca gttcgtttat ttttatattt taattatata atgttagcac agccgcactt
     2581 ttgttgtcaa ctatttaatg tatatttacc atgttaaaca gttgacggct gtatacatgc
     2641 tattcagcca tccgagcacc cataaaaaat aaaaaattat aaatcggttt aggttgaaac
     2701 aacaaacggt ataattttaa aactgtttga aaaactataa aagtaacaaa gttttaagga
     2761 gcaaaattta tgaaagcgac aggaatagtg cgtagaatag atgatctcgg cagagttgtc
     2821 attcctaagg aaattcgcag aacaatgcgt atcagagagg gtgacccgct cgaaatattt
     2881 acggcgtcgg acggagaagt tatctttaaa aaatactcgc cgattggcga actgtccgaa
     2941 tttgccgacc aatatgccga ggtgctttgc agagctaccg acttgcctgt tataataacg
     3001 gaccgtgacc acgtgatttc cgtttcggga atatcgaaaa aagaatatct tgagagaaga
     3061 atatcgtcgg aacttgaaac actcatggaa agcagaacca actataccgc atctgccgat
     3121 gaagagccta tttacccgat aagcggaatg agccgtgaag cggcggtagc ttatccgata
     3181 ataggcagcg gcgacgtttc gggctgcgtt gtgctgcttt taaattccga cggttctctt
     3241 ccctctgaga cggagcgcaa actggtagct gttgcggcgt cttttctcgg caaacagatg
     3301 gaggaataac cgtttgcacg cctaaagaac ggaaaatata caaagcaaaa gaaaaaccca
     3361 ccgctttttg cggtgggtaa atttcttatt aagccttaat tattcggcaa cagcgtcttt
     3421 aagagcttta cctgctttga aagccggaac cttggaagcg ggaacggtca tggtctcgcc
     3481 tgttctcgga ttgcgaccct gcttagctgc acggttgcga acctcgaagg taccgaagcc
     3541 aacgagctga atcttgtcgc ctgctttaag agcgtcggtt atagcaccga gaactgccga
     3601 tactgctgcg tcagcgtctt tcttagagag gtcggtcttt tcagcaactg ctgcgataag
     3661 ttctgtctta ttcattttgt tttcctccgt tttacaatgt tttatttatc cgtcgcttcg
     3721 ccctcggcgt gcgaacagaa agctgaattt aattttgcct cgtcccaaag cttatcaagc
     3781 tccgtgatag aggacttttc catattgata ttccgctctt ttacaaggcg ttccaccgtc
     3841 gcaaagcgag acaggaattt atcggatgaa cgggtaagag cctcttcggc atcaacactt
     3901 atgaaacgtg ccgcattgac tgccgagaaa agtaaatcgc cgagctcctc aaacgcattt
     3961 tcgctgtcgc cgttttttac agcggctttg aattcggcgc tctcttcgtc tattttgtct
     4021 atcgcaccgc tcacatcggg ccaatcgaag cccaccttgc tcgccttttc ctgaagcttc
     4081 tgtgcacgca taagtgccgg aagctctctc ggaacggaca gcattttatc ggtaatggtt
     4141 ttgcgctttt tcgacttaga cttaatggcg tcccaattac ttaaaacctc gtccgtggac
//
LOCUS       DBMDVO010000032         4175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9101_length_4175_cov_1.398625, whole genome shotgun sequence.
ACCESSION   DBMDVO010000032 DBMDVO010000000
VERSION     DBMDVO010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9101_length_4175_cov_1.398625"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="ACZPE2_00905"
     CDS             complement(<1..1493)
                     /locus_tag="ACZPE2_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1318435.1"
                     /translation="MKRPLAVTGFTMLFTLLVLSGSNDKAISAVLLAALIAFVVFMCI
                     KKVRRDKTLPFAALSAAVAVLLLMSNNAYYLGKAKPLNGKEVIISGTLSDLPYEKDGS
                     FYYVLKADTVNGEKSSLSFRVVSKAPLCIDVTDEVSLSAKAFLLGGDSDEILKYYKSK
                     SLFLGAHSPENISIKRGEKKNIGTMLLLLRQKLTSETLCLLPNTYGGLVAGLCYGDKS
                     NIPRNVTTAFSAAGVSHLLAVSGLHLSVWSSLLYMIMRKLRVSRRISSASSIVFIIFF
                     AILTGMNPPVVRAGTMIGLVFAADLFKREADSLNSIGLSLILLLSFNPYMSTSLSLWL
                     SLAATLGMMYFLKPLSRALDRPIANVKQNIFTTVYKSVSSLVAVSLAVTAASLPVFAV
                     SLPTVSTVSVLSNLLMVTVGSVCMTSGGLGALFMLIKLDIIGKPLVLLSGITAKYLTE
                     TAVKISEFHHALLPIDSIFTRISIAAALIVFAVVLLLGYKNKTAVKR"
     gene            complement(1496..1834)
                     /locus_tag="ACZPE2_00910"
     CDS             complement(1496..1834)
                     /locus_tag="ACZPE2_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR1318436.1"
                     /translation="MDNCLFCKIIAGEIPSKKVYEDDLCYAFYDINPQSDTHFLVVPK
                     QHISGADKITGENSAVVAHIFEVISSLTKQLGLSSYRIINNCGEGAGQTVMHLHFHVL
                     SGGNLGERLV"
     gene            complement(1848..>4175)
                     /gene="alaS"
                     /locus_tag="ACZPE2_00915"
     CDS             complement(1848..>4175)
                     /gene="alaS"
                     /locus_tag="ACZPE2_00915"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1318437.1"
                     /translation="IPWAWEFCTKVLEMDPEKLYISVYEEDDEAYDIWTKDIGVDPSH
                     MVRFGKEDNFWEHGAGPCGPCSEIYYDRGEKYGCGKPDCKVGCECDRYIEFWNLVFTQ
                     FENDGNNNYSRLAHPNIDTGMGLERLACISQDVDNLFEVDTVQNIMKHIMKIAGVKYH
                     DDEKKDVSLRVITDHVRSTTMMISDGVMPSNEGRGYVLRRLLRRAARHGRLLGIDRPF
                     LYEVAETVIKENDTAYPSLLEKHDFIINVIKAEEENFAKTIDTGLNMLEEMVKTTNGK
                     VMSGADAFKLSDTYGFPLDLTKDILDEKGMTVDEQEYTALMTEARKKAREAHKDAGAE
                     AWKSSGNATKGMDKTPFVGYETLSGDSEILAVVVDGEKKQVATEDDNITLVLSETPFY
                     AESGGQVGDVGVIKGNGFEFTVENTTKTHEGVVLHHGYISDGETVSCGDKVHAEVNRS
                     VREATMRNHTAAHLLQAALRKILGTHVEQAGQLVNSEAVRFDFTHFSALSADELRKVE
                     NTVNEVIMSAVPVVTSEMPIDEAKKLGAMALFGEKYGDIVRVVKADDFSVEFCGGTHV
                     KNTGSIGLFKIVSESSVASGVRRIEAVTGNNIMKYIDKNNELIAETAKNLKLTNYHEL
                     ASRAAALSAELKEKEREISSLEAEIAASKTADLMKDAKQIGGVRLVTADIGEAGADAL
                     RSLCDKALESGDDIIAVFAGKNTEKGTASFACRVGKKAIECGAHAGNIVREVARVAGG
                     AGGGKPDSAMAGAKDISKIPEALKKASEIVGAMLA"
BASE COUNT         1059 a         1061 c          920 g         1135 t
ORIGIN      
        1 attcgcttta cagctgtctt gtttttgtat ccgagcagca aaacgactgc aaaaacaata
       61 agcgctgcgg cgatactgat tcttgtgaaa atcgagtcta tcggtagcaa agcgtggtga
      121 aattccgata ttttaacagc tgtttccgtg agatatttcg ccgtaattcc cgatagcaaa
      181 acaagcggtt ttcctataat atcaagcttt atgagcataa aaagtgcccc gagtccgccg
      241 cttgtcatac aaaccgatcc gacggtaacc ataagtagat ttgaaagcac ggatactgtt
      301 gagacggtcg gcaggctgac ggcaaagaca ggcaatgaag ctgccgttac cgctaagctc
      361 acagctacga gagacgatac ggatttatac acagttgtaa agatgttttg ctttacatta
      421 gctatcggtc tgtcgagtgc ccgtgacagc ggctttaaga agtacatcat accgagcgta
      481 gcggcaagcg acagccacag gcttaaagag gtactcatat acggattaaa tgagagcaat
      541 aaaataagcg aaagtcctat tgaattcaga gaatcagcct ctcgcttgaa caaatcggca
      601 gcaaatacaa gacctatcat tgtgcctgct cttacaacgg gaggattcat tcccgtgagt
      661 attgcgaaga aaataataaa cacgattgac gacgcagaag agatacggcg cgacacacgc
      721 agttttcgca taatcatata caaaagcgag gaccacacac tcaggtgaag ccccgaaacg
      781 gcaagcaaat gcgaaactcc ggctgccgag aacgccgtag tgacatttcg tggaatattc
      841 gacttgtcac cgtagcaaag ccctgccaca agtccgccgt atgtgttcgg cagaaggcac
      901 agcgtctcgc tcgtcaattt ttgacgaagc aaaagcaaca tagtgccgat gtttttcttt
      961 tctcctcttt ttatgcttat attttccggt gaatgcgcgc cgagaaacaa agattttgac
     1021 ttatagtatt ttagtatttc gtcgctgtcg ccgcctaaga ggaatgcttt tgccgaaagg
     1081 ctgacctcgt ccgtcacgtc tatacagagc ggagcttttg ataccacgcg aaatgacaga
     1141 gagctttttt cgccgttcac cgtatccgct ttcagcacat aataaaagct tccgtccttt
     1201 tcgtacggca aatccgagag ggtaccgcta atgattactt ctttgccgtt tagcggcttt
     1261 gcttttccta aatagtatgc gttatttgac atcaggagca aaacggcaac cgccgcgctg
     1321 agagccgcaa aaggcaaggt tttatctcgc ctcacctttt taatacacat aaaaacaaca
     1381 aaagcgataa gcgcagccaa aagcactgcg cttatcgctt tatcatttga accgctcaat
     1441 acaagaagtg taaagagcat tgtaaatccc gtaactgcaa gcggccgttt cataatcaaa
     1501 cgagtctttc gccgagatta cctccgctga gaacgtggaa atgaagatgc atcaccgttt
     1561 gaccggcacc ctcgccgcag ttgtttataa tgcgatagga tgagaggccg agttgctttg
     1621 taagagagga tatcacctca aaaatgtgcg caacaacagc ggaattttcg cctgttatct
     1681 tatccgctcc gctgatatgc tgtttcggga cgacgagaaa atgcgtatcc gactgcggat
     1741 ttatatcata aaacgcatag cacaaatcgt cttcatatac tttttttgac ggaatttctc
     1801 cggctattat tttacagaaa agacaattat ccattttcat cctccgttta ggcaagcata
     1861 gcgccgacta tttcggaagc ctttttaaga gcttccggta tttttgaaat atcctttgct
     1921 ccggccatgg cactgtcggg ttttccgccg cctgcaccac ctgcaacgcg tgctacctcg
     1981 cgaactatat tacctgcatg agctccgcac tctatggctt ttttaccgac gcggcaagcg
     2041 aacgacgctg tgcccttttc ggtatttttg cctgcgaaca cggcgataat atcatctccg
     2101 ctttcaagtg ctttgtcaca aagcgagcga agagcatcgg ctccggcttc gcctatatcg
     2161 gctgtgacaa ggcgtacacc gcctatctgc tttgcatctt tcataaggtc ggcagttttc
     2221 gatgctgcaa tctctgcctc caaagacgat atttcgcgtt ccttttcttt aagctccgca
     2281 gagagagccg cggcacggga cgccaattca tgataatttg taagcttaag gtttttggcg
     2341 gtttcggcaa taagctcatt gttcttgtca atgtatttca taatattatt gcccgtaaca
     2401 gcctctattc tgcggacgcc cgacgctacc gatgactcgg atactatttt gaagagccct
     2461 attgatccgg tatttttaac atgggttccg ccgcagaact cgacagaaaa atcatctgcc
     2521 tttacaactc ttacgatatc gccgtatttt tcgccgaata atgccattgc gccgagcttt
     2581 ttcgcctcgt caatcggcat ttcgcttgta acaaccggca cggccgacat tattacttcg
     2641 tttacggtat tttcaacctt tctgagctca tccgccgaga gagccgagaa atgagtaaag
     2701 tcaaatctga cagcctcgct gtttacaagc tggcctgcct gctcaacatg agtgccgagt
     2761 attttacgca gagccgcctg taaaagatgc gccgcggtat ggttacgcat ggtggcttca
     2821 cgtaccgaac gattgacctc agcgtgaacc ttgtcgccgc acgatacagt ctcgccgtcc
     2881 gagatgtagc cgtggtgaag aactacgccc tcatgtgttt tagtggtgtt ttcaacggta
     2941 aattcaaatc cgttgccctt gataacgccg acgtcgccga cctgaccgcc gctttccgca
     3001 tagaacggag tttcggatag cacaagtgtt atattgtcat cttccgttgc aacctgtttc
     3061 ttttcgccgt caactacaac agcgagtatc tcgctgtcgc ccgaaagtgt ttcatagccg
     3121 acgaacgggg ttttatccat tcccttggtg gcattgcccg agctcttcca agcctctgcg
     3181 cctgcgtcct tgtgagcctc acgagctttt ttgcgagcct cggtcataag agccgtatac
     3241 tcctgctcgt caacggtcat acccttttcg tcaagaatgt cctttgtaag gtcaagcggg
     3301 aaaccgtaag tatcggagag cttaaacgca tcggcaccgc tcataacctt accgttggtg
     3361 gttttaacca tttcctcgag catattaagg cccgtatcaa tggtctttgc gaagttttcc
     3421 tcctccgctt taataacatt tatgataaag tcgtgctttt caagaagact cgggtaagct
     3481 gtatcgttct ctttaataac cgtctcggca acctcataaa ggaagggtct gtcaataccg
     3541 agcaatctgc cgtggcgtgc tgcgcggcgc aaaagacgtc ttaaaacgta gcctctgccc
     3601 tcgtttgacg gcataacacc gtcggatatc atcattgtgg tgcttcttac gtgatctgtt
     3661 ataacacgaa gagatacatc ctttttctca tcatcgtgat atttcacgcc ggcaattttc
     3721 ataatgtgct tcattatatt ctgaaccgta tcaacctcaa agaggttgtc aacgtcctgc
     3781 gatatgcaag cgagtctttc aaggcccata ccggtatcta tattgggatg cgcaagacgc
     3841 gagtaattgt tgttaccgtc attttcgaac tgagtgaata cgaggttcca aaattcgata
     3901 taacggtcgc actcacagcc taccttgcag tcgggcttgc cgcagccgta cttttcgccg
     3961 cggtcataat atatttcaga gcacggtccg cacggacccg ctccgtgctc ccagaagtta
     4021 tcctccttgc cgaaacgaac catgtgcgac gggtcaacgc ctatatcctt agtccatata
     4081 tcgtaagcct cgtcatcttc ctcataaacg ctgatataga gcttttcggg atccatctcc
     4141 aataccttgg tgcaaaattc ccatgcccac ggaat
//
LOCUS       DBMDVO010000033         4169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9120_length_4169_cov_1.070551, whole genome shotgun sequence.
ACCESSION   DBMDVO010000033 DBMDVO010000000
VERSION     DBMDVO010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9120_length_4169_cov_1.070551"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(43..>264)
                     /locus_tag="ACZPE2_00920"
     CDS             complement(43..>264)
                     /locus_tag="ACZPE2_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318438.1"
                     /translation="SQQIGHTDKDEQSGKDEDKKEEGKVKADDDAADESGVHGLEQSE
                     KDIEDAPSDAEKPKKKKEKKRFGFFNRHS"
     assembly_gap    265..274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<275..1246)
                     /gene="ftsY"
                     /locus_tag="ACZPE2_00925"
     CDS             complement(<275..1246)
                     /gene="ftsY"
                     /locus_tag="ACZPE2_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1318439.1"
                     /translation="MGIFSKINFGLTKTRNKMAGAIDDLLDSVGEINDDLYTELEELL
                     VMHDVGVQTSLEIIEELRKRVPEKKIKNPTMVKDEIKEIVAEKLYGGEDMGLITIPSI
                     IFVIGVNGVGKTTTIGKLAAMYKSEGKKVILGAADTFRAAAIDQLQIWADRAGVDIVK
                     HSEGADPAAVVFDTINAAKARNCDIVIIDTAGRLHNKKNLMDELAKMYRIIDRELPYS
                     DREVLLVLDATTGQNAVNQAREFMNAAEITGIVLTKLDGTARGGVVLTIKNELGIPVK
                     FIGVGEQIDDLQPFNPRAFADGLFEKIDYNEEDEKDGISQEDVAPDGQ"
     gene            complement(1832..>4169)
                     /gene="smc"
                     /locus_tag="ACZPE2_00930"
     CDS             complement(1832..>4169)
                     /gene="smc"
                     /locus_tag="ACZPE2_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1318440.1"
                     /translation="IRSRISAIDESMGTRKDDYDALLKRKADAEASLKEIQDEIVSVK
                     NAIDGYTLRFENRGKKADSVKLAIDEKQRELHKGQDRVRLLEDLEKNMEGYFGAVKAV
                     MKESGRGALRGIYGPVSQLITVKDKYSAAIETALGAAVQNIVVDNETDAKRAMGFLKE
                     RRAGRATFLPITAIKGRVLSEQGLDDQYGFVSIASDLVSYDNKYSEIIRWLLGRTAVA
                     EDIDSAIAIAKKYSYRFRIVTLDGQVINAGGSMTGGSRVQNAGILSRGNEIERLKGSL
                     ASMQKELDGMLSDYKLLSEDASAAKAELEGAEGDLLRAKEENIRREGELKLASDKLAS
                     VSSGVKELLEEKETLEKRIESVSSGAEAARSQIDELKETLENKEKELESITGDSKTLQ
                     KNREEVASKAAEIRLRIVSLQKDVEANTDEITRLKNRKTGHLDRLSELDGEIREIEEK
                     NDELRALTERLSADEKALKANHGDAQNQINELISQRDELEKQANDLRLHERAKSEERE
                     RLSGDIARLEERKIAMRNEYDNLTSKLYDEYQLTRREAAALEIEIDDYSLAAKRLAEL
                     KSQIRALGSVNVSAIEEYKEVSERYEFLSGQINDVETSRAELNKMIDDLTGKMAEQFR
                     EQFNRINSCFGQTFIELFGGGKAELRLEDERDVLGSDIEIKVQPPGKNVQNINLLSGG
                     EKGLSAIALLFAILKVTPAPFCIFDEVEAALDDVNVSRYAQYVRRMTKNTQFILITHR
                     RGTMEEADVLYGVTMQEKGVSKLLELKTAEMAKKLGLA"
BASE COUNT          879 a         1080 c          952 g         1248 t
ORIGIN      
        1 agcggcgaga gtattctctg aagctcggcg cgttttttca tattatgagt ggcgattaaa
       61 aaatccaaag cgctttttct cctttttctt tttgggcttc tctgcgtcgg acggagcgtc
      121 ttctatatct ttctcactct gttccagtcc atgaactccg ctttcatctg ctgcgtcgtc
      181 gtcagctttg actttgccct cttctttttt atcttcgtct ttgccgcttt gttcatcctt
      241 atcagtatgt ccgatctgtt gtgannnnnn nnnnttgccc gtcaggcgcc acatcttctt
      301 gcgaaattcc atctttttcg tcctcctcgt tgtaatctat tttttcaaac agaccatcgg
      361 caaaagccct ggggttaaac ggctgcaaat catcaatctg ctcgcccacg cctataaatt
      421 taacgggaat cccaagctcg tttttaatag tgagaacaac tccgcctcga gccgtaccgt
      481 caagctttgt taaaactatg ccggtgattt cggcggcgtt cataaattca cgcgcctgat
      541 ttacggcgtt ctgtcccgtt gtggcgtcaa gcaccaacag cacctcgcgg tccgaatacg
      601 gaagctcgcg gtctataatg cggtacattt tggcgagctc atccataagg ttctttttat
      661 tgtgaagtct tcccgcggtg tcaattatga caatgtcgca gtttcttgcc tttgcggcat
      721 ttatcgtgtc aaaaacaaca gccgcagggt ctgctccctc ggagtgcttt actatatcaa
      781 caccggctct gtccgcccaa atctgaagct ggtcgattgc cgcggcacgg aatgtgtcgg
      841 ccgctcctaa aatgaccttt ttgccctcgg acttgtacat cgccgccagt ttgcctatgg
      901 tcgtggtttt acctacgccg ttgacgccta ttacaaatat gatggacggt atggttataa
      961 gccccatatc ctcgccgccg tatagctttt cggcgacaat ctccttgatt tcatccttaa
     1021 ccattgtcgg atttttgatt tttttctcag gcacgcgctt gcgaagctcc tcgattatct
     1081 caagcgaggt ctgcactccg acatcgtgca taacgagcag ctcttcaagc tcggtgtaaa
     1141 ggtcgtcgtt tatctcgccg acgctgtcga gcaaatcatc aatcgctccc gccattttat
     1201 ttcgggtctt tgtaagtccg aaatttattt tgctgaaaat tcccatttgt ttattccttt
     1261 cggataagaa atttttgatt tcaatcaaat agattatgcg aatattttaa gcacagccac
     1321 agtatcgccg cgtttttcgc cgcaaataaa gctccgagcg gcgtgtagtt gatttttgtc
     1381 tatacgcgag ttcgggtgga caaaagccgc atggtgcgca agatttacgc tgcattttat
     1441 ttgaagtata tatcaaaccc gtttagtgta aagatttggt gcaaggtggt ttttgtgaac
     1501 cccgacgcgt actacggtac gcgagttcta gtgaacaaaa gccgcatggt gcgcaagatt
     1561 tacgctgcat tttatttgaa gtatatatca aacccgttta gcgtaaagat ttggtgcaag
     1621 acgggttttc gtaagccccg acgcgtacta cggtacgcaa gttcaggtga acaaaaagcc
     1681 gcattgtgcg caagatttac gctgtgttct gtttaaatta tatatcaatg atgtttagtg
     1741 taaagatttg gtgcaagacg ggttttccga gccccgacgc gtatgtatat acgcgagttt
     1801 gggtgaggaa aattcgtatg gcgccgaaga tttacgctaa accgagtttc ttcgccattt
     1861 cggctgtctt taactcaagt aatttggata cacccttttc ctgcatagtc acaccgtaca
     1921 gtacatcggc ttcttccatg gtgccgcgac gatgggtaat taagatgaac tgggtgtttt
     1981 tagtcatacg gcgaacatac tgagcgtaac gcgaaacgtt gacgtcgtca agtgccgctt
     2041 caacctcgtc gaaaatacag aacggcgcgg gggttacctt gagtatcgca aaaagaagcg
     2101 caatggcgga aagtcccttt tcaccgccgg agagcaagtt gatgttctga acgtttttgc
     2161 cgggcggctg aaccttgatt tcaatgtcgc tgccaagcac gtcgcgctcg tcctcgagtc
     2221 gaagctctgc cttacctccg ccgaacagct caataaaggt ctgcccgaag catgagttga
     2281 tacgattgaa ctgctcgcga aactgctctg ccattttacc ggtcaaatca tctatcatct
     2341 tattaagctc cgcgcgcgag gtttcgacat catttatctg ccccgataag aattcgtaac
     2401 gctcggatac ctctttgtat tcctctattg cggagacgtt tacggagccg agagcgcgga
     2461 tttgcgattt aagctctgcg agacgctttg ccgcaaggga gtaatcgtca atttctattt
     2521 cgagagccgc agcctcgcga cgggtgagct gatactcgtc gtaaagcttg ctcgtcagat
     2581 tgtcatattc gttgcgcatt gctattttgc gctcttcaag acgcgctatg tcgccgctga
     2641 gacgttcgcg ctcctcggat tttgcgcgct cgtgaagtct taagtcattc gcctgctttt
     2701 caagctcgtc acgctgtgaa atcagctcgt ttatctgatt ttgcgcgtct ccgtgatttg
     2761 ctttaagagc cttttcatca gccgaaagcc gctcggtcaa ggctctgagc tcatcgtttt
     2821 tctcttcgat ttcgcgtatt tctccgtcaa gctccgagag acggtcaagg tgaccggtct
     2881 tacgattttt aagccttgta atctcgtccg tgttcgcctc aacatccttt tgaagcgaaa
     2941 ctatgcgaag tcttatttca gccgcttttg atgcgacctc ctcgcgattc ttttggagag
     3001 tcttgctgtc gccggttata ctctcaagct ctttttcctt gttttccagc gtttctttaa
     3061 gctcgtcgat ttgcgaacga gccgcctcgg cacccgagct tacggactcg attctctttt
     3121 cgagagtctc cttttcctcc aaaagctctt taacgcccga ggaaacggac gcgagcttgt
     3181 cgcttgcaag tttcaattcg ccctcgcggc ggatattttc ttctttagct cttaaaaggt
     3241 cgccctcggc gccctcaagc tccgcctttg cggcagaggc gtcctccgac aaaagcttgt
     3301 aatccgagag catcccgtca agctcttttt gcattgaagc aagagagcct ttaagtcttt
     3361 caatctcatt gcctcggctg agtatgcctg cattttgaac gcgagagccg cccgtcatgg
     3421 agccgccggc atttataacc tgaccgtcaa gcgtgactat gcggaagcga tacgaatact
     3481 tttttgcgat agctatggcg gaatctatgt cctcggcaac tgccgtgcga cccaagagcc
     3541 agcggattat ttccgaatat ttattgtcat acgacacaag gtcggaggca atgcttacaa
     3601 agccgtattg atcgtcgagc ccctgctctg agagaactct gcccttaatt gcggttatcg
     3661 gcaaaaatgt ggctctgcct gcgcgacgct cctttaagaa gcccatggcg cgctttgcgt
     3721 cggtttcatt atctacaaca atgttctgca cagccgctcc gagagccgtc tcgatagcag
     3781 ccgaatattt atccttaacc gttatgagct gagatacggg accgtatatg cccctgagcg
     3841 cacctctgcc cgactccttc ataacggctt ttaccgcgcc gaagtagccc tccatatttt
     3901 tttcgagatc ctcaagaagg cggaccctgt cctgcccctt gtgaagctcg cgctgttttt
     3961 catctatggc aagctttacg ctgtccgcct ttttgccgcg gttttcaaat cgcagagtat
     4021 agccgtctat tgcgtttttg acggaaacta tctcgtcctg tatttccttg agtgatgctt
     4081 ccgcgtcggc tttgcgtttt aaaagcgcgt cgtaatcgtc cttgcgagtt cccatggact
     4141 cgtctatcgc ggaaattctc gaccttatc
//
LOCUS       DBMDVO010000034         4154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9165_length_4154_cov_1.207747, whole genome shotgun sequence.
ACCESSION   DBMDVO010000034 DBMDVO010000000
VERSION     DBMDVO010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9165_length_4154_cov_1.207747"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            147..1430
                     /locus_tag="ACZPE2_00935"
     CDS             147..1430
                     /locus_tag="ACZPE2_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1318441.1"
                     /translation="MGYNPDSAIETKCVQAGWQPKNGEPRVLPIYESTTYKYDTSAEM
                     AKLFDLEPGYMYTRLANPTNDAVANKIACLEGGVGAVLTSSGQAANFFALINILGAGD
                     HIVSSATIYGGTFNLLNVTMRKIGVEVTFVDPRASEEEINAAFRDNTKAMFGETIANP
                     SLDVLDIEKFAKIAHSHGVPLIVDNTFPTPINCRPFEFGADIVTHSTTKYMEGHASTI
                     GGCVVDSGNFDWYQNDKFPGLTTPDESYHGVTYAKDFGKNGYIVKIVAQLLRDYGSTP
                     SPFNSFLLNIGLESLAVRVERHCENAQKVAEYLEKNDKIAWVNYPGLKSSKDYELAKK
                     YLPHGTCGVISFGVKGGREKAEQFMNSLKLAAIVTHVADARTCVLHPASSTHRQLSDE
                     ELTECGVTPDLVRLSVGIENVNDIIADIDNALKAI"
     gene            complement(1534..2058)
                     /locus_tag="ACZPE2_00940"
     CDS             complement(1534..2058)
                     /locus_tag="ACZPE2_00940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR1318442.1"
                     /translation="MMEAIENMKTRRSIRKFKPDAVPQELMDKIIEAGLYAPSGMNKQ
                     SPIIIAVTDRKMRDEISEMNREIGGWQEGFDPFYGAPAILIVLANKECRTAVYDGSLT
                     MGNLMLAAHNFGLGSCWIHRAKEEFESEKGKAILKKLGINGEYEGIGHCALGYADEEP
                     KEHIIAENRVYHIK"
     assembly_gap    2083..2092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2181..3533)
                     /gene="rlmD"
                     /locus_tag="ACZPE2_00945"
     CDS             complement(2181..3533)
                     /gene="rlmD"
                     /locus_tag="ACZPE2_00945"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1318443.1"
                     /translation="MSLSKNQNVELYIDSFTSEGSGVGRYENMAVFVSGAAAGDKVTA
                     HIIKVKKTYAVARVEKVLRPAKSRIMPACPYFPSCGGCAFQHISYEKEKEHKKKRVED
                     AFSHIAHINIEAETLLSDNKTTCYRNKAQYPVSFDRQLKIGFFAPRSHRVVDCENCIL
                     QPPIFADIVKIFRKFIIKNGITVYDSEKHTGLLRHIYLRLAEATGEVMVCAVINGEDL
                     PHKNELIKRLTEIHSVKSIVLNINRDRTNVILGKECVTVWGNDCIYDVLCGVKVRLSP
                     LSFYQVNHDMAQQLYEKAAEYACLSGKETVLDMYCGTGTIGLSMAKKAKRIIGAEIVP
                     EAVLDAKRNALENGIENAEFICADAGEAAQELSSRGIKPDVVLLDPPRKGCSEMLLAE
                     IVKMSPERIVYVSCDPSTLARDCFRLKDLGYETKRLAAADLFPRTVHVESVALLTKQK
                     "
     gene            complement(3533..3712)
                     /locus_tag="ACZPE2_00950"
     CDS             complement(3533..3712)
                     /locus_tag="ACZPE2_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318444.1"
                     /translation="MKLTFWDAVNPFAGEYIFDEPQQRLEFIITAAIAAVLLLLFCIC
                     LKRAIKKHKNDSEKK"
     gene            3999..>4154
                     /locus_tag="ACZPE2_00955"
     CDS             3999..>4154
                     /locus_tag="ACZPE2_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1318445.1"
                     /translation="MDKFVITGGHRLKGEVTVSGAKNAVVAILPATILAEDVCRIENI
                     PNISDVTA"
BASE COUNT         1118 a         1023 c          880 g         1123 t
ORIGIN      
        1 agaccccgta ccgtttatga tttatcataa aaacgacgaa aaggacagcg gcgttgactc
       61 tataaacgaa gagagcgcaa aaacgaccgg cctgtttata gagcatggcc ccgacattat
      121 gaagagattt ttggaggatt aaaaaaatgg gatacaatcc tgattccgca atagagacaa
      181 aatgcgtaca ggcaggctgg cagccgaaaa acggcgagcc gcgagttctg cccatttatg
      241 aatctacaac atacaaatat gacacaagtg ccgaaatggc aaagcttttt gaccttgagc
      301 cgggttatat gtatacgcgc cttgcgaacc cgacaaacga cgcggtcgca aataaaatcg
      361 catgccttga gggcggtgtc ggcgcggtgc tcacatcatc gggtcaggcg gcaaatttct
      421 ttgcgcttat aaacatactc ggtgcaggcg accatatagt aagctccgcc actatttacg
      481 gcggcacatt taatctttta aacgtgacta tgcgtaaaat cggtgttgag gtcacattcg
      541 ttgacccgag agcgagtgaa gaagaaataa acgcagcctt ccgcgacaac acaaaggcga
      601 tgttcggcga aacaatagcg aatccgtcgc ttgatgtatt ggacattgag aagttcgcaa
      661 aaatcgcaca ttcgcacgga gttccgctta tagttgacaa cactttcccc actcccataa
      721 actgccgtcc gtttgaattc ggcgcagaca tagtaactca ctctaccaca aaatatatgg
      781 aaggtcacgc ttctacaata ggcggatgcg tcgttgacag cggcaatttc gactggtatc
      841 aaaatgataa attcccgggg cttacaactc cggatgagag ctatcacgga gttacatacg
      901 ctaaggactt cggcaaaaac ggctatatcg taaagatagt agcacagctt ttgcgcgact
      961 acggctcaac tccgtcgccg tttaattcgt tcctcttgaa cataggtctt gagtcgctcg
     1021 cggtacgtgt ggaacgccac tgcgaaaacg cacaaaaggt tgccgaatat cttgaaaaga
     1081 acgataaaat cgcttgggta aattacccgg ggcttaaatc aagcaaggat tacgagcttg
     1141 ccaaaaaata tttaccgcac ggtacctgcg gcgtaatttc cttcggcgta aaaggcggaa
     1201 gagaaaaagc cgaacagttt atgaactcat taaagcttgc cgccatagtc acgcacgtcg
     1261 ccgatgcgag aacctgcgtt cttcaccccg caagctccac tcaccgtcag ctttcggacg
     1321 aggaactcac ggaatgcggc gtaacccccg acctcgtaag actttccgtc ggcattgaaa
     1381 atgtaaacga tataatcgcc gacatagata atgctcttaa agccatataa cccacataac
     1441 acataaaaca cataaaaaca gccgtccgaa aacctgattc ggacggctgt tttttactta
     1501 taaatcatct gttttaagca gtaatttttc gctttatttg atatggtaaa cgcggttttc
     1561 ggctatgata tgctccttcg gctcttcgtc ggcatagcct aaggcacaat gtcctatccc
     1621 ctcatattcg ccgtttatac cgagcttttt gagaatcgcc ttaccctttt cggattcaaa
     1681 ttcctccttt gcgcggtgaa tccagcatga gccgagtccg aaattatgtg cggcaagcat
     1741 aaggtttccc attgtaagcg agccgtcgta aacagctgtg cggcactcct tatttgccag
     1801 cacaataagt attgcgggcg cgccgtaaaa cgggtcaaag ccctcctgcc aaccgcctat
     1861 ttcgcggttc atttcggata tttcgtcgcg cattttcctg tcggtaacgg caatgattat
     1921 cggtgactgc ttattcatac ccgacggcgc ataaagtccc gcctcgataa tcttatccat
     1981 aagctcctgc ggtactgcgt cgggcttaaa ctttcttatg cttcttctcg ttttcatatt
     2041 ttcgatagct tccataattt tatcctttcc gacaattcag gtnnnnnnnn nnttaagtag
     2101 cttttcgggc acaaaaaatc gtatcctcat catcaatata gcacatttgt caagcacagc
     2161 ccgtaaaaaa caaatacacc ttatttttgc tttgtaagca atgcaacgct ctcaacatga
     2221 acggtacggg ggaaaaggtc ggcggcggcg agccgttttg tctcgtatcc gaggtcttta
     2281 agcctaaagc agtcccttgc gagagtagac gggtcgcacg acacataaac tatgcgttcg
     2341 ggcgacattt tcacgatttc cgccaaaagc atttcggaac agcctttgcg cggcggatca
     2401 agcagcacca catcgggctt tattccgcgt gacgacagct cctgtgccgc ctctcctgcg
     2461 tcggcacata taaattcggc gttttctatg ccgttttcga gggcgttgcg ctttgcgtca
     2521 agcacagcct ccggaactat ctccgcccct attatcctct ttgctttttt tgccatggaa
     2581 agacctattg tacccgttcc gcaatacata tcgagcacgg tctctttgcc gctgagacag
     2641 gcatactctg ccgccttttc ataaagctgc tgtgccatat cgtggtttac ctggtagaac
     2701 gacagcggcg acaatctgac cttaactccg caaagcacgt catatatgca gtcgtttccc
     2761 caaacagtca cgcactcttt accgagaatc acattggtcc tgtcacggtt gatatttaaa
     2821 actatgctct taaccgagtg tatttcggta agccttttaa tcagctcgtt cttgtgcggc
     2881 aaatcctcac cgtttataac ggcgcatacc ataacctcgc ccgtagcttc ggcaagacgg
     2941 agatatatat gccttaaaag ccctgtatgc ttttcggaat catagaccgt tataccgttt
     3001 ttaattataa attttctgaa aatcttaact atatccgcaa atatcggcgg ctgtaatatg
     3061 cagttttcgc aatctaccac gcggtgcgag cgcggagcga aaaagcctat tttaagctgc
     3121 ctgtcaaacg atacgggata ctgtgcctta ttgcgataac aggtcgtttt attgtcggac
     3181 agaagcgttt ccgcctctat attgatatgg gcaatatgag aaaacgcatc ttcgacgcgc
     3241 tttttcttat gctccttttc tttttcgtac gatatgtgct gaaaagcgca gccgccgcat
     3301 gacggaaaat acgggcacgc aggcattata cgcgattttg cgggccttag caccttttcg
     3361 acgcgggcga cggcatacgt ctttttaacc ttaattatat gcgccgttac cttatcgcct
     3421 gccgctgcgc ccgaaacgaa aacagccata ttttcatatc ttccgacgcc gctgccctcg
     3481 ctcgtaaagc tgtctatata gagttctaca ttctgatttt tactgagtga catcactttt
     3541 tctccgaatc gtttttgtgt ttttttatcg cccttttaag gcaaatacaa aacagcagca
     3601 aaagcaccgc ggctattgcc gccgtaatta taaattcaag tctttgctgc ggctcatcga
     3661 atatatattc gccggcaaac gggtttaccg catcccaaaa tgtaagtttc attttcttta
     3721 agcaccgttt cttttttaga cattgtaaca caaaaaaatg caaatataaa gcgtattata
     3781 tcgaaaacgg acgtattcaa acaaatgttt tattattttc aacaaaaaat caccatatac
     3841 aaaaaaagag taaaaaataa cttttaattt acgctgctcg gtggtataat aaccatatta
     3901 tatgcctcga ggtacggcga agtgcgcccg tttttgcctc aggcttgctt gattttaatt
     3961 ttttctttac ataaaaactt tagggagtga accgtccttt ggataaattc gttattaccg
     4021 gcggtcatag gcttaaggga gaggttactg tcagcggcgc aaaaaacgcc gttgttgcca
     4081 tccttccggc tacaatttta gccgaagatg tatgccgcat agaaaatata ccgaatatca
     4141 gcgacgttac cgca
//
LOCUS       DBMDVO010000035         4147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9193_length_4147_cov_1.130005, whole genome shotgun sequence.
ACCESSION   DBMDVO010000035 DBMDVO010000000
VERSION     DBMDVO010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9193_length_4147_cov_1.130005"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="ACZPE2_00960"
     CDS             complement(<1..479)
                     /locus_tag="ACZPE2_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1318446.1"
                     /translation="MASELKELVFKMNRLDCYKRYAMHKMLRDSGVYFGQPPVLDYLS
                     EHGECSQKELSDGINVSPASVAVSVKRMQKSGLITKISDENDLRCNRIALTEKGKQRT
                     EYIHSQFDKLDKRMFKGFTKEELSLLNSFLNRLIDNISEDAPSEKTIFEMMMRSHEKG
                     "
     gene            707..1237
                     /locus_tag="ACZPE2_00965"
     CDS             707..1237
                     /locus_tag="ACZPE2_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIR1318447.1"
                     /translation="MKKLIQNHKGGIITDRKSLKKREKDFCREYVYCGNPKDAALRAG
                     YTVFPEMCGIRLLTEKRIKDEIAELEGKLAATRAEALCGYRRIAFGNISDAVKLTLES
                     DGDRLDAEKLDLFNVAEIKKPRGGGMEIKFFDRLKALEKIEQLSLSESDKYSVSLYKA
                     IENSAKASAGGNNDDC"
     gene            1227..2477
                     /locus_tag="ACZPE2_00970"
     CDS             1227..2477
                     /locus_tag="ACZPE2_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIR1318448.1"
                     /translation="MTVDAFKSFSKKQLILLNWWTDSSPYKNRDAIICDGSVRSGKTV
                     CMFLSFIFWAFYMFSDSSFAVCGKTITSLRRNIITPMLPMLRSLGFTYEELVSKNMII
                     IKKDGRENRFYIFGGKDEGSAALIQGMTLSGVMLDETALMPRSFCEQAIARCSVDNSR
                     LWFNCNPENPAHWFYTEWIKKAVDKNCLYLHFTMDDNPSLSEEIKQRYKNLYSGSFYE
                     RFIEGKWVSCDGAVYPFFDSSYIYDVPAGITKYFVSCDYGTVNPTSMGLWGEKDGTFF
                     RVDEFYHDSKQKGFQMTDEEYYCSLTALIGDRKVEYAVVDPSAASFIECIKRHGKIRV
                     VRADNDVVKGIRKTSDALKSGRIKICKPCRDAIREFSLYRWDESAKYDAPRKENDHAM
                     DDIRYFVMSLENSADGFFAVSVER"
     gene            2494..3633
                     /locus_tag="ACZPE2_00975"
     CDS             2494..3633
                     /locus_tag="ACZPE2_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR1318449.1"
                     /translation="MKLFRRGKRIEAPVTAAAQGTASGLFPAVNSYTPLVLCQNTLYK
                     SLMEAVPIINAAVHKIIRLTGGFTVETGSAACDKALAEFLSDIPCDSGERSIYSFLDT
                     YFEQLLIYGTAVGEMLTDEYGNIRYLYNARPDDVSLLRDPNDFSRILVCRADAVPTPV
                     KHQERILFTALDAEPGSLYGTSVLYGLPFVSSVLLKIFEATKSNWDRVGNVRFAVTYK
                     PDGEALSKSFAKERAELIASEWSEAMKSGSVKDFVAVGDVDIKVIGADNQIPDSEIPV
                     REMLEQIVAKLGIPPFLLGLSWSTTETMSKVETDVLTTELWHYRRHLTPVIERICSTF
                     LRNLGSSLKPIVVWDDITLQDEVENARARLYSAQAKALEKEEKTD"
     gene            3630..>4147
                     /locus_tag="ACZPE2_00980"
     CDS             3630..>4147
                     /locus_tag="ACZPE2_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HIR1318450.1"
                     /translation="MNKSFESQTDENELSKEDLALINTFTQKEMTKDDIFSFCVILCD
                     NEIDRDYERFTEDSLHKLADFFIGKTAIRDHSMKSSDQSARTYKTEVIKDSTRKNSLG
                     EDYVYLKAWCYMPRIKKNEELIEEIKAGIIKEVSVGCAVKSCICSVCGKELGKSECSH
                     VRGGVYNGRLCYG"
BASE COUNT         1156 a          926 c         1016 g         1049 t
ORIGIN      
        1 cctttttcgt gtgatctcat catcatttca aaaattgttt tttcgctcgg agcgtcctcc
       61 gaaatattgt caataagacg attcaaaaag ctgttgagaa gtgaaagctc ctcttttgtg
      121 aagcctttga acatcctttt gtcaagcttg tcaaactgac tgtgtatata ttccgtacgc
      181 tgcttgccct tttcggtaag agctatccgg ttgcagcgca ggtcgttttc gtctgaaatt
      241 tttgtgataa gtccgctctt ttgcattcgt tttacggata ccgccaccga tgccggcgaa
      301 acatttatgc cgtcggaaag ctctttttgc gagcattcgc cgtgctcact tagataatcg
      361 agcaccggcg gctggccgaa atacacgccg ctgtcgcgaa gcattttatg catggcatag
      421 cgtttatagc agtcaagcct gttcatctta aagaccaatt ctttaagttc cgaagccaaa
      481 gccttttcct cctttttaaa tataagataa ccgatttata gttagtcggt taattgttaa
      541 tcggtttact tattgtaata ccgatttttt ttattgtcaa taacattaca aaaaaatctt
      601 gaatcattaa agtccgcctg catcaacggg tttgagatgt taatagacac atatctgaaa
      661 tgtgtcgcag cgttttcgga gcattgctcc ctttttgccg ttatgtatga aaaaacttat
      721 acaaaatcac aagggaggaa ttattacgga cagaaaaagt cttaaaaaac gcgaaaaaga
      781 tttttgccgc gaatacgttt actgcggtaa tccgaaagat gcggcgctga gagccggata
      841 caccgtcttt cctgaaatgt gcggtattcg actcctaacg gaaaaacgta tcaaagatga
      901 gatagccgag cttgaaggca agcttgccgc tacgagagcc gaggcgcttt gcggttaccg
      961 ccgtattgcg ttcggtaaca tttctgacgc tgtaaagctc acactcgaaa gtgacggtga
     1021 caggcttgac gccgaaaagc ttgacctttt caatgtagcg gaaatcaaaa agccgcgcgg
     1081 cggaggaatg gaaatcaagt tttttgaccg tttgaaggca cttgaaaaaa tcgagcagtt
     1141 gtcattatca gaaagcgaca aatattccgt ttcgctttat aaggctatcg aaaattcggc
     1201 aaaggcttcc gccggaggga acaacgatga ctgttgacgc ctttaaaagc ttctcgaaaa
     1261 agcagcttat actgctgaat tggtggacag actcttcgcc gtataaaaac cgagatgcga
     1321 taatctgcga cggctctgta agaagcggta agacggtgtg tatgtttctg tcttttatat
     1381 tttgggcatt ttatatgttt tcagacagca gctttgccgt ttgcgggaaa accatcacct
     1441 ctctgcgacg caacataata acaccgatgc tcccgatgct gcgttctctc ggcttcacat
     1501 acgaggagct tgtttcaaaa aatatgataa tcataaaaaa agacgggcga gaaaaccgtt
     1561 tttacatttt cggaggtaag gatgaaggct cggcggcgct gatacagggt atgactcttt
     1621 cgggcgtcat gctcgacgag actgctctta tgccgcgctc cttttgcgag caggcaatag
     1681 ctcgctgctc cgttgataat tcgcgccttt ggttcaactg caaccccgaa aatccggcgc
     1741 actggtttta cacggaatgg ataaaaaagg cggtagataa aaattgtcta tacctgcatt
     1801 tcacaatgga cgacaatccc tcgctgtcgg aggagattaa acagcgttat aaaaatcttt
     1861 acagcggttc attttacgag cgatttattg aaggtaaatg ggtcagctgt gacggcgcgg
     1921 tttacccttt tttcgacagc tcatatatat acgacgtgcc tgcggggatc acgaaatatt
     1981 ttgtttcctg cgactacggc acggtaaatc cgacatccat ggggctttgg ggcgaaaaag
     2041 acggcacctt tttcagagtg gatgaattct accacgattc aaagcaaaaa ggctttcaga
     2101 tgaccgatga ggagtactat tgttcgctta cagctcttat cggcgacaga aaagtggaat
     2161 acgcggtagt tgacccctcg gccgcaagct tcattgaatg tataaaaagg cacggcaaaa
     2221 tacgcgtggt gcgtgccgac aacgacgttg taaagggcat acgcaaaacg tcggacgcgc
     2281 tgaaatccgg caggataaaa atctgcaagc cgtgccgcga cgccatacgt gagttttcat
     2341 tataccgctg ggacgagagt gcaaaatacg atgccccgcg caaagaaaac gaccacgcca
     2401 tggacgacat aagatatttc gttatgtcac tcgaaaactc cgccgacggc ttttttgccg
     2461 tttcggtgga acgatgaagg gaggtaatta tgcttgaagc tattcagaag aggcaagcgc
     2521 attgaggctc cggttaccgc cgccgcacaa ggcacagcct cggggctttt tcccgccgtc
     2581 aacagctata caccgcttgt gctgtgccag aacacgcttt ataaaagcct tatggaggca
     2641 gttcccataa taaatgcggc tgttcacaag ataatccgat tgacgggcgg cttcacggta
     2701 gaaacgggaa gcgccgcctg cgataaagct cttgccgaat ttttgtccga cataccgtgc
     2761 gactcgggcg aaagaagcat ttattcgttt ttggatactt attttgagca gctcttgatt
     2821 tacggcacgg cagtcggtga aatgcttacg gacgaatacg gaaacatacg ctatctctac
     2881 aatgcaagac cggacgatgt cagtctttta agagatccta acgacttttc aaggattctc
     2941 gtatgccgcg cagacgcagt gccgactccg gtaaagcatc aggagagaat actgtttacg
     3001 gcacttgacg cagagcccgg ctcgctctac ggtacctctg tgctttacgg cctgccgttt
     3061 gtaagctcgg tactgcttaa aatattcgaa gccaccaaat ccaactggga cagagtcggc
     3121 aatgtacgat tcgccgtcac ctataagccg gacggagaag cgttgtcgaa atcatttgct
     3181 aaagagcgcg ccgagcttat agcaagcgag tggagcgaag ctatgaagag cggcagcgtc
     3241 aaggactttg tcgccgtcgg agacgttgat ataaaggtca taggtgccga taatcaaatt
     3301 cccgacagcg agattcccgt acgcgagatg cttgagcaaa ttgtcgcaaa gcttggtata
     3361 ccgccgtttt tgctcggact ctcgtggtca accaccgaga cgatgtccaa ggtggagacg
     3421 gacgttttga ctacggagct gtggcattac aggagacatt tgacgcctgt aatagaaagg
     3481 atttgctcaa catttcttcg caatttgggc tcatccttaa aaccgatcgt tgtatgggac
     3541 gacatcacgc ttcaggacga ggtggaaaat gccagagcaa ggctgtattc ggcacaggca
     3601 aaagcacttg aaaaggagga aaaaaccgat tgaacaaatc atttgaaagt cagacggatg
     3661 aaaacgagct gtcaaaagag gatttggcgc tcataaacac atttactcaa aaggaaatga
     3721 cgaaggacga tatattctct ttctgcgtaa tactctgcga caacgaaatc gaccgtgatt
     3781 acgagcgatt cacggaggac tctcttcata agcttgccga cttttttata ggcaaaaccg
     3841 caatacgaga tcacagcatg aaaagctccg accagagcgc gcgaacctat aagaccgaag
     3901 tcattaagga cagcacgcgc aaaaattctc tcggtgagga ctatgtatat ttaaaggcat
     3961 ggtgctatat gccgaggata aagaaaaacg aggagctcat tgaggaaatc aaggcaggca
     4021 ttataaaaga agtgagcgtg ggctgcgcag ttaaaagctg catctgctcc gtttgcggaa
     4081 aagagctcgg caagagtgaa tgttcacacg tcagaggcgg cgtttacaac ggcaggctct
     4141 gttacgg
//
LOCUS       DBMDVO010000036         4068 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9458_length_4068_cov_1.006050, whole genome shotgun sequence.
ACCESSION   DBMDVO010000036 DBMDVO010000000
VERSION     DBMDVO010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_4068_cov_1.006050"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(297..524)
                     /locus_tag="ACZPE2_00985"
     CDS             complement(297..524)
                     /locus_tag="ACZPE2_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318451.1"
                     /translation="MDIQYSVKPAGSEIRKEKIKSRLFGLFITLFIMSALFIKMFVFV
                     PMLNEHPTFGDDRNGVARVIASDNIPQNIMS"
     gene            1028..2461
                     /locus_tag="ACZPE2_00990"
     CDS             1028..2461
                     /locus_tag="ACZPE2_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318452.1"
                     /translation="MSKSEEAMHGITFKDKIGYAMGDMGGLLTFGLVSAFLNMFYTDV
                     LGISAARITVLMIVARIWDAVNDPMWGSFIDSRKPTKYGRFRPYILWASLPLAVGAFF
                     MFFKIPGLSDNRYLIYAYITYIFYGMMYTGVNIPYGSLASVITDDEIERSSLSMWRSI
                     GAGIGGLPASIILPLIVYTTVTNENGTTAKVLDGTKLSLSVAVLSVLTVIIFFLHFKL
                     TKERITLPPTQNQKDYSIGKAMRALLKNKPFIALCIVSMLLICFQMYTQTVYNYLFKN
                     FYEKPGLYALVTVCTYLPTVLLLPFMGKLIRKYGKKEICSAGILFALIVNIAMFVIKF
                     TPAVSNPYVFLVLSFFSGAGQTFLIMQIWALVTDVTDYHEYISGRREEGTSYSFYSFV
                     RKLGQTVAGAGAAGVLGAIGYDGKLAVQSPEVLTRLYDIATIVPAILLALIFVVLTFG
                     YKLSKEQLDELHNVLRERREKAETESK"
     gene            complement(2540..>4068)
                     /locus_tag="ACZPE2_00995"
     CDS             complement(2540..>4068)
                     /locus_tag="ACZPE2_00995"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1318453.1"
                     /translation="PPKWSFDYCASTMAYTDAPNSEEQLYGFLRKLDTLNMSCSGFYL
                     SSGYTSIGDLRCVFNWNYDKFPNPAKFIERFNSEKIHLIPNIKPAFLTSHPMYNEIAE
                     KGLFVKNEDGTPFVTEFWDGMGSYIDFTLPEGFKFWHDNVAAKLLDYGIDATWNDNNE
                     FDIKDTDAVANGFKDGSVKASRIRPILTYLMVASSYSAQTEKYPGKRPFLSTRSGSSA
                     VRRLATTWSGDNFTSFHDLRYCHYIGMTLSLSGFFFYGHDLGGFSGDMPSRELLLRWM
                     QHGIFEPRFTIHSWNSDGSATMPWSYPDAIPAARKIMAERKKLIPYLYNSAFLSVEND
                     VPMTAPLLLYYGDKEIDCDSTSFLVGRDILAACILDEGVSETEVYLPKNEIWYLNDKV
                     YDGGTSVMLNIPSDGEVPYFVRGGSVIATDEGEYGFKSSEKLRLTVYPLRSGSFKSEF
                     FTDDGESFAYKTGDCVHLLFTVDCDSEYVRVRIENIGEKSFIPDIVLCDADDRILTVE
                     "
BASE COUNT         1124 a          908 c          863 g         1173 t
ORIGIN      
        1 attgatctga tgatttctgc cattttagct cctcgctttg tatctattgc acaagtattg
       61 ctttctttat ttgtacacat cttacagccg cgttgtcgtt ttgtcaatag ttgagctgaa
      121 ttttttgtca ctttctacaa cttttgtata ttagtacaaa acgacggttt tgatgtcgta
      181 aatttagtgc gattgcacaa cacaagcagc atacgccata taatgtaata tataaaaaac
      241 gaccgtcgtg cactcggcac ggcggtctca acagtaatag tttcaaaagt acggctttac
      301 gacataatat tctgcggaat attgtcagac gctataacgc gagcgacacc gttgcggtcg
      361 tcaccgaatg tcggatgctc gttcagcatg ggaacaaaga caaacatttt tataaacaga
      421 gcggacatta taaacaatgt tataaaaagt ccgaaaagac ggctcttgat tttttctttt
      481 cgaatttccg aaccggcggg tttaacgcta tactgtatat ccatatataa tatctcctgt
      541 caaatcttat tgtactctta tcaactgcaa tacaatatat atcatcgcaa aattacaaac
      601 aagagtcgaa attattaaga ttaggttaaa aagtgttttt cgtttaaacc cggacttaaa
      661 aggtgataat gttaacttgt tgcctctttg ggtatattgc ttagtttttg cgattgacta
      721 ctgcatattt ttgtattata attccattat gtcatttatg ctctgctgcg gatttttcgc
      781 aaacgaaatc aacttatcag cagctatgta aagtataatg ctttacagga ttttgctttt
      841 tataggcgtg aaagatgagc tgctacggct tataaaggcg ctatatagcg gatttctttc
      901 aagttccgaa agcaaaaggt ctgtgtgtaa atgtagtttg ctttacacta cccggtatca
      961 tcctgtcagg tatcggaaag caaacagcaa atgtaaagtg tataaaacac aaaaacggag
     1021 gaattgtatg agcaaatcag aagaagctat gcacggcata acctttaaag acaaaatagg
     1081 ctacgccatg ggagatatgg gcggactgct gacattcgga cttgtctccg catttttgaa
     1141 tatgttttac acggacgttc tcggaatatc cgcggcgcgc ataaccgtac tgatgatagt
     1201 tgcgagaatt tgggacgcag taaacgaccc tatgtggggt tcttttatcg actcaaggaa
     1261 gcccactaaa tacggaagat tcaggccgta catactttgg gcatctctgc cgcttgcggt
     1321 aggtgctttc tttatgttct ttaaaattcc gggcctcagc gacaaccggt atcttattta
     1381 cgcatatatc acgtacatat tttacggcat gatgtacacc ggcgtaaata ttccctacgg
     1441 ctcgctcgcc tcggtaataa cagatgatga aatagagcgg tcttcgcttt cgatgtggcg
     1501 aagcatcggt gcaggcatcg gcggtcttcc cgcctctatt attttaccgc tcatcgttta
     1561 tactaccgtg acaaatgaaa acggaacaac cgcaaaggta ctcgacggca caaagctctc
     1621 gctgtcggta gccgttctct cggttctcac tgtaataatt ttcttcttac actttaaatt
     1681 gacaaaagag agaataacct taccgccgac gcagaatcaa aaggattata gtatcggcaa
     1741 agcaatgcgc gctcttttaa aaaacaagcc gtttatcgcg ctttgcatag tatcaatgct
     1801 gcttatctgc ttccagatgt atactcagac cgtttacaac tatctcttta agaatttcta
     1861 tgaaaagccg ggtctttacg cacttgttac cgtatgtaca tatttgccga cggtactgct
     1921 tttgccgttt atgggcaagc ttataagaaa atacggcaaa aaagagatat gctccgcagg
     1981 aatactcttt gctcttattg taaacatagc aatgttcgtc attaaattca ctccggctgt
     2041 gagtaatccg tatgtatttc tcgttctctc gttcttctcc ggggccggtc aaaccttcct
     2101 tattatgcaa atctgggcac ttgttacgga tgtcacggac tatcacgaat acatatcggg
     2161 cagacgagaa gaaggcacat cttacagctt ttattcgttt gtacgcaagc tcggtcaaac
     2221 agtggccggt gcaggtgcgg caggcgttct cggagctata ggctatgacg gcaagctcgc
     2281 cgttcaatca cccgaggttc tgacaaggct atatgacata gcaacaatag tccccgcaat
     2341 attgcttgct cttatattcg ttgtattgac gttcggctat aagctttcaa aggagcagct
     2401 agatgagctt cacaatgttt tgcgcgaaag aagagaaaag gcagaaaccg aatcaaagta
     2461 aaaacaggtc ttgatgacaa aatccccgga tatgccgaag caagcatatc cggggctctt
     2521 ttttgtgatt tacaatgtat tattctaccg tcagtattct gtcgtcggcg tcgcaaagca
     2581 caatatccgg gataaatgat ttttcaccga tattctcgat acggactctg acatactcgc
     2641 tgtcgcaatc cacggtgaac agcaagtgaa cgcaatcgcc ggttttatac gcaaagctct
     2701 cgccgtcatc tgtaaagaat tccgatttaa agctgccgct ccttaacgga tacacggtaa
     2761 gccttaattt ttccgaggat ttgaacccgt actccccctc atcggtggct ataacgcttc
     2821 cgcctcgcac aaaatacggg acctcaccgt ccgacggaat attcagcatt acggacgtac
     2881 cgccgtcgta tactttatcg tttaaatacc aaatttcgtt tttggggaga taaacctcgg
     2941 tttcgctcac tccttcatcg agaatacacg cggcgagaat atctctgccc acgaggaacg
     3001 atgtgctgtc gcagtctatc tctttgtcac catagtagag taacagcgga gctgtcatcg
     3061 gcacatcgtt ttcgacagat aaaaaggcgg aattataaag atacggaatg agtttttttc
     3121 tttccgccat tatttttcgt gcggcaggaa tggcatccgg atagctccac ggcatagttg
     3181 cggagccgtc agaattccac gaatgtattg taaatctggg ctcgaaaatg ccgtgctgca
     3241 tccatcttaa aagcagctca cgtgacggca tatcgcccga aaatccgccg agatcatgcc
     3301 cgtagaagaa aaatcccgac aaagacagcg tcatacctat gtaatggcaa taacgcaggt
     3361 catgaaatga tgtaaaattg tcacccgacc aggtggttgc gagacgtctt acggcactgc
     3421 tgccgcttcg tgtcgataaa aacggtcttt tgcccggata tttttctgtt tgcgcagagt
     3481 acgaggaggc gaccataaga tacgtgagta tcggtcttat tctgctcgcc tttacgctac
     3541 cgtctttaaa gccgtttgcc acagcgtcgg tatcttttat atcaaattcg ttgttatcgt
     3601 tccaagtggc gtctattccg tagtccagaa gctttgccgc aacattgtcg tgccaaaact
     3661 taaagccctc gggcagagta aaatcaatgt aactgcccat accgtcccaa aactcggtta
     3721 caaacggtgt gccgtcttca ttctttacaa aaagcccttt ttcggctatt tcgttgtaca
     3781 tcgggtgcga cgtcaaaaaa gcaggcttga tattgggtat caagtgaatt ttctcactgt
     3841 taaaacgctc aataaacttt gcggggttcg ggaatttatc ataattccaa ttaaaaacac
     3901 accgcaagtc gccgatggag gtatagcccg aggacagata aaatccgctg cagctcatat
     3961 ttaaggtatc aagctttctg agaaatccat acaactgttc ttcggaattc ggcgcgtcgg
     4021 tataagccat ggtgctggca cagtaatcaa agctccattt cggaggca
//
LOCUS       DBMDVO010000037         4037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9590_length_4037_cov_1.204522, whole genome shotgun sequence.
ACCESSION   DBMDVO010000037 DBMDVO010000000
VERSION     DBMDVO010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9590_length_4037_cov_1.204522"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="ACZPE2_01000"
     CDS             complement(<1..428)
                     /locus_tag="ACZPE2_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1318454.1"
                     /translation="MSDDKKLIEVQHLQQYFPAGGRGKKKKYVQAVDDVSFYIKKGET
                     LGLVGESGCGKTTVGRTLLRLYEPTDGTIIYDGDVIFDKKKKIAVNMLPYRRKMQMVF
                     QDPYASLDPRMTIGDIVGEAIDIHHLAANKKERRDKIISLL"
     gene            complement(421..1425)
                     /locus_tag="ACZPE2_01005"
     CDS             complement(421..1425)
                     /locus_tag="ACZPE2_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318455.1"
                     /translation="MSEKLLEIKDEKLSFFTPAGEVKALNGVSFSMDEGEVLGIVGES
                     GSGKSVTAYSIMGLTAYPGKLIGGTIRFNGHEIEKMTDKDFRKIRGNEVSIIFQDPMT
                     SLNPVYTIGNQITEVIRLHTDKDKKQAYERAKELLELVGINEPTKRLKQYPHELSGGM
                     RQRVMIAIALACEPKLLIADEPTTALDVTIQAQILDLMQDLRKKLGMSIIMITHDLGV
                     VASMCEKIAVMYAGHIVEYGTADEIFYEPKHEYTKGLIKSIPKLNVESKERLVPIEGQ
                     PVDLLNPPAGCPFAPRCSKCMKICLKQMPPKTVLSDTHYSHCWLLQKEEFEEGEKDCE
                     "
     gene            complement(1441..2481)
                     /locus_tag="ACZPE2_01010"
     CDS             complement(1441..2481)
                     /locus_tag="ACZPE2_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318456.1"
                     /translation="MNKKFPSLHLKADDFIPATTTEKENLVVMRDSVNFWKDGLRRLR
                     KNKVAMISLVFILLIVVFAYILPSFWPYSYEQQIKYSENLSPFQYSETEQAQIDAGQK
                     VFPHICGTDKLGRDFAVRLMMGTRISLTVGLVCAALVLIVGSLFGAIAGFAGGWVDNI
                     MMRFTDILYTIPDILLIILLATSLKPRLEALSANPGFTWMQRMGPNLIAIFLVFVLIY
                     WVGMARITRSQILVLKESEYVTAARALGASNSRIIRKHLLTNCIGTLIVTTTLQIPSA
                     IFTESYLSFLGLGVTAPMPSLGSLATEAVKGMNTYPYLLFLPALLISVIILAFNLLGD
                     GLRDAFDPKLKN"
     gene            complement(2497..3414)
                     /locus_tag="ACZPE2_01015"
     CDS             complement(2497..3414)
                     /locus_tag="ACZPE2_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318457.1"
                     /translation="MTKYIIKRIAMGLLSIFIVATLTFFLMNLVPGGPFVAEKSISKA
                     AQEALAAKYGLDKPLFEQYITYITDFIKGDMGVSLRQRGRTVSDIIFSKFPVSAKLAG
                     IAVLVSLLVGIPLGCLSAYNRGKFADNFIIVLATCGIAIPSFIISVILLYTFGSKLNL
                     LPTVGLNTLSSYIMPVTALSVYPTAYITRLMRSSLLDVMGQDYIRTAKAKGLSNFKIL
                     FKHALRNAILPVITYVGPMLASLMTGSFIVEQIFAIPGLGREFVSAIVNRDYTMIMGT
                     TIVLATLIIVANVIVDILYKIIDPRIKLK"
     misc_binding    3725..3968
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1059 a          889 c          890 g         1199 t
ORIGIN      
        1 agaagcgata tgattttgtc gcgtctttcc tttttgttgg cggcaagatg atgaatatca
       61 atggcttcgc ctacaatatc tcctatcgtc atacgcgggt caagacttgc atacgggtcc
      121 tgaaatacca tctgcatctt tcttcggtag gggagcatat tcaccgcgat ttttttcttt
      181 ttgtcaaata tgacgtctcc gtcatatatt attgttccgt ctgtgggctc gtaaagcctt
      241 aaaagagtgc gccctactgt ggttttaccg cagccggatt cgccgacgag cccgagagtt
      301 tcgccttttt ttatataaaa cgaaacatcg tctaccgcct gaacgtattt tttctttttg
      361 cctctgcctc cggcaggaaa gtactgctgc aagtgctgta cttctatgag ctttttatcg
      421 tcactcacag tctttctctc cttcctcaaa ttcttctttt tgaagcagcc agcaatggct
      481 gtaatgtgtg tcgcttaaaa ccgttttcgg cggcatttgc tttaagcata tcttcatgca
      541 ttttgaacat ctcggtgcaa acggacaccc tgccggcggg ttgagaagat cgaccggctg
      601 cccttcaatc ggtacaagcc tttccttact ttcgacattc agcttcggaa tggattttat
      661 aagacccttt gtgtattcgt gcttcggctc ataaaatatc tcgtctgccg tgccgtattc
      721 aactatatga cccgcataca taactgcaat tttttcgcac atactcgcga caacgccaag
      781 gtcgtgagtt atcataatta tgctcattcc gagcttttta cgcaaatcct gcataaggtc
      841 aagtatctgc gcctgaattg tgacgtcaag agccgttgta ggttcgtcgg ctattaaaag
      901 cttcggctcg caggcaagag ctatggcaat cataactctc tggcgcatac cgcccgaaag
      961 ctcgtgcgga tactgtttaa gtcgcttggt aggctcgtta atgcctacaa gctccaaaag
     1021 ctccttagcg cgctcatatg cctgtttttt gtctttatcc gtatgcagcc ttataacctc
     1081 tgttatctgg ttgcctattg tgtaaacggg attaaggctc gtcatagggt cctgaaatat
     1141 tatggaaacc tcgtttcctc ttatctttct gaaatcctta tcggtcatct tttcaatttc
     1201 gtgaccgtta aatctgattg ttccgcctat aagcttgcca gggtatgcgg taagccccat
     1261 tattgaatat gctgtgacgg attttcccga accagattca ccgactattc cgagaacctc
     1321 gccttcatcc atcgaaaacg aaacgccgtt aagtgctttg acttcgcctg cgggagtaaa
     1381 gaacgacagc ttctcgtctt ttatttccaa aagtttttca ctcaaaatat ttaccctcct
     1441 ttagttcttg agtttcgggt cgaacgcgtc gcgcagcccg tctccgagaa ggttaaaggc
     1501 aagtatgata acgcttatca gtagcgccgg taaaaacagc agatatggat aagtgttcat
     1561 accctttacg gcctccgtgg caagagaacc gagcgacggc atgggagccg taacgccgag
     1621 tccgaggaaa ctcaaatagc tttcggtaaa tattgcggag ggtatctgaa gtgtggttgt
     1681 tactataaga gtacctatgc agttcgtcaa aaggtgcttt ctgattatac gcgaattgct
     1741 tgcaccgagt gctcttgccg cggtaacgta ttcggactcc ttgagtacaa gtatttgaga
     1801 acgtgttatt cttgccatac ctacccagta tatcaaaacg aatacaagga atattgctat
     1861 aaggttcggt cccattctct gcatccaagt aaaacccgga ttagcagaga gcgcttcgag
     1921 tcgcggcttt agggaggttg caagcagaat tattaaaagt atatcgggaa tggtatataa
     1981 aatgtcggta aaacgcatca ttatattatc gacccaaccg ccggcaaaac cggctattgc
     2041 accgaacagc gagccgacta taagaaccag cgctgcgcac acaagaccta ccgtaaggct
     2101 tattcttgtg cccatcataa ggcgaacggc aaagtctctg ccgagcttat ccgttccgca
     2161 gatgtgagga aatacctttt gacccgcgtc tatttgcgcc tgttctgttt cgctgtactg
     2221 aaacggggag aggttctcgc tgtattttat ttgctgctca tagctgtacg gccaaaagct
     2281 gggaagaatg taagcaaaaa ccactatcag taaaataaag acaagactta tcatggcaac
     2341 cttgttttta cgcagacgtc tgaggccgtc cttccaaaag ttgacgctgt ctcgcattac
     2401 aacaaggttt tctttttccg tcgttgtagc cggtataaaa tcgtctgcct tcaaatgcag
     2461 actcggaaat tttttgttca tcggcgatgc cgctccttac ttaagtttga ttctcgggtc
     2521 gattatttta tacagtatgt caactatgac gtttgcaact atgataagtg tcgcaaggac
     2581 aattgttgtt cccattatca tggtgtagtc gcggttgact atggccgaaa caaattcacg
     2641 gccgagaccg ggtattgcaa atatctgttc tactataaac gaacccgtca taaggcttgc
     2701 gagcattggt ccgacgtatg ttattacggg gagtattgcg tttctgagag catgcttgaa
     2761 gagtattttg aagtttgaaa ggcctttggc ttttgccgtt cttatataat cctgccccat
     2821 aacgtccaaa agacttgagc gcataagccg cgttatatat gccgttgggt aaaccgacag
     2881 cgccgtcacc ggcattatat atgatgacag cgtgttaaga cctaccgttg gcagtagatt
     2941 cagcttgctg ccgaatgtat acagtaaaat tacgctgatt ataaagctcg gtattgcgat
     3001 accgcacgtc gcgagtacta taataaagtt atcggcaaat ttacctcggt tgtatgccga
     3061 aagacatccg agcggtatgc ctacaagaag agagacgaga acagctattc cggcgagctt
     3121 tgccgatacg gggaatttag aaaaaataat gtccgatacg gttcttcctc tttgacgtaa
     3181 gcttacgccc atatcgccct tgataaaatc ggtaatatag gttatatatt gctcaaataa
     3241 aggtttgtcc aatccgtatt ttgcagcgag agcttcctgt gcagccttgc ttatggattt
     3301 ttccgcaacg aacggaccgc cgggaacaag gttcataagg aaaaacgtca gcgtggcaac
     3361 aatgaaaatg ctgagcaagc ccattgctat gcgcttgatg atgtacttgg tcaaatgata
     3421 gaccccctgt taatttgtgt atttttttca aaagacatag aagtcttttt atattattgt
     3481 ctcaaataac caattattgc taaatcacag gtattcttga cgacattatt tgtacatttt
     3541 agcacaatgg ctcactaaaa tcaagatttt tgctttgtat ttatttaact attccgcaaa
     3601 acaaagcatt ttttcgtgat atgtaccgat ataagacatt ttgccccata aataacgatt
     3661 taaaaatatt tgtaattttt tcttgacaaa gaaagaacgg cgtgataaca ttgtggcaac
     3721 atctaaaaaa cctgtgatta agaagagtag ctttacttaa agcttgcaga gagcggttgt
     3781 cggtggaaat accgcagggg agagtacagc gaatggactt atgagggcgg cacgaaagcg
     3841 tttgcgagta gcggccgacg ggttccgtac ccgttatcag agcggcttat atgtaagtat
     3901 aagaaacgag cggtcggttt ttccggcaat ttgggtggta ccgcaggatt caaagtcttg
     3961 tcccatatat gggataagac tttttttatt tgcttattaa ggagaaaaga atgcaatatt
     4021 atttaagacg atggcga
//
LOCUS       DBMDVO010000038         4036 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9598_length_4036_cov_1.164676, whole genome shotgun sequence.
ACCESSION   DBMDVO010000038 DBMDVO010000000
VERSION     DBMDVO010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9598_length_4036_cov_1.164676"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            12..116
                     /locus_tag="ACZPE2_01020"
     CDS             12..116
                     /locus_tag="ACZPE2_01020"
                     /inference="COORDINATES: protein motif:HMM:NF036726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318458.1"
                     /translation="MNDYCEDTSLQKSAKEYMLGGNKLGFGTGYIKRQ"
     gene            206..964
                     /locus_tag="ACZPE2_01025"
     CDS             206..964
                     /locus_tag="ACZPE2_01025"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1318459.1"
                     /translation="MKKMISWNVNGLRAAVGKNFKEVFDSLDADIFCIQETKLQEGQI
                     DLEFDGYKSYWNYAEKKGYAGTAIFTKEEPKDVFYGINEPFYDNEGRVITLEFENYFF
                     LTCYTPNSQSELKRLEYRMSWEEKFLTYLKKLEKTKPVILCGDLNVAHKEIDLKNPKT
                     NMGNAGFTNEEREKFGTLLDSGFIDTFRYFHPDEPGRYSWWSYRFNARKNNAGWRIDY
                     FLVSEELKDKLVSADILSDIQGSDHCPVELVADI"
     gene            complement(974..1654)
                     /locus_tag="ACZPE2_01030"
     CDS             complement(974..1654)
                     /locus_tag="ACZPE2_01030"
                     /inference="COORDINATES: protein motif:HMM:NF021101.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HIR1318460.1"
                     /translation="MKRKNCAKRVFFSFAVFVMSVSVLVFTFTCRMRPVITRYSETVA
                     ERILLNSTNKAVAEVLAESETAYNDIVKTERNAEGNVTSLHVDTVKINLLQSNISLAV
                     SEIAAENETFSISIPIGTFLGSEYTNGYGPRVKFNAQLTSGTVVAFSNEFREAGLNQV
                     IHRIVFNIETSGRLVMLGKTDGLSVTASAVVAETVIVGVLPEALTNVIGSPSELAGII
                     NDYGAAFD"
     gene            complement(1775..2017)
                     /locus_tag="ACZPE2_01035"
     CDS             complement(1775..2017)
                     /locus_tag="ACZPE2_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318461.1"
                     /translation="MKKKDRIIKKYLAVLTVVLCAFALFAGVSESANRTSQLTRGVSD
                     SVYTEKQILESVAKEAKNGREFLNAIDEKITEMIFG"
     gene            complement(2092..3249)
                     /gene="spoIIP"
                     /locus_tag="ACZPE2_01040"
     CDS             complement(2092..3249)
                     /gene="spoIIP"
                     /locus_tag="ACZPE2_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR1318462.1"
                     /translation="MKKQKRHTGKIPKKRNEVLVALPIIIILAFVCGSRFGNAVGRTA
                     VIGAGLMLPEGAVLSAKSSAVRSIEKSVSAQSSQQNAVISLPSEKNEQSESVAGSTPT
                     VPRDIVKMMADAEKQYASANHDGTIVEKKFDASSATSTYKNISVRNTTPSHSINIESS
                     LNGECNLDIADKSAPTVLIFHTHTTECYQLMDKGWYTRDFELRSDDSSRNMVRVGDAV
                     SEELQKAGIGVIHDTEIHDRAYTGAYDRSRETILKIMEENPSVKIVLDVHRDAIEQSD
                     GTRIKPTTTVNGRKAAQIMIIAGCEDGKVTSFPTWEQNLTFALKLQQTAETMYPGLMR
                     PILFSARKYNMDVTPCSVLLEFGSDSNTIAEAEYSGHLMGKAIVEFINSNV"
     gene            complement(3411..>4036)
                     /gene="gpr"
                     /locus_tag="ACZPE2_01045"
     CDS             complement(3411..>4036)
                     /gene="gpr"
                     /locus_tag="ACZPE2_01045"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1318463.1"
                     /translation="KLLPKNTSGVLVVGLGNSDITPDALGPKTAKDIFSTRHITKSLA
                     EEIGLPSLRPVSSASPGVLGQTGIESAEMIRGIMNETSPDAVITVDALSARSIKRLGC
                     TVQMTNTGIVPGSGVGNHRAEISRKTLGVPVIAIGVPTVVDAAALVFDITGNENIPQP
                     ERERAEKMMVTPREIDVMISRASRLLALAINSALQPDMDMKTLLSLV"
BASE COUNT         1028 a          979 c          896 g         1133 t
ORIGIN      
        1 gaaaaagcga tataaacgac tactgcgaag atacctcttt acaaaagagc gcaaaggaat
       61 atatgctcgg cggaaacaag cttggcttcg gcacgggcta cataaaaagg cagtaggctg
      121 caaaaataca tcttgcgttt tgcagcgccg cgcctaaagc aaatattatt ataaaaaagg
      181 agcacccgaa agggcaatta ccgcaatgaa aaagatgata tcatggaacg taaacggact
      241 gcgcgccgca gtcggcaaaa atttcaagga ggtatttgat tctcttgatg cggatatctt
      301 ttgcatacag gaaacaaagc ttcaggaagg tcaaatcgac ctggaatttg acggctataa
      361 aagctattgg aactatgccg aaaaaaaagg ctatgcgggc acagcgatat tcacaaaaga
      421 ggaaccaaaa gacgtatttt acggcataaa cgagccgttt tacgacaatg aaggcagagt
      481 cataacgctc gaatttgaaa attatttctt tctaacctgt tacacaccga attctcaaag
      541 cgaactcaaa aggcttgaat acagaatgtc gtgggaagaa aaattcctga catatcttaa
      601 aaagcttgaa aagacaaagc ctgttattct ttgcggcgac ctaaacgtgg cacacaaaga
      661 gatagacttg aaaaatccga aaacgaatat gggtaatgcc gggtttacca acgaagaaag
      721 agaaaaattc gggacccttt tagacagcgg ttttatagac acctttaggt attttcaccc
      781 tgacgagcct ggcagatact cttggtggtc ttacagattt aacgcgcgta aaaacaacgc
      841 aggatggaga atagactact ttcttgtatc ggaagaactc aaagacaagc tcgtttccgc
      901 cgacattctc tcggatatac aaggctcaga ccactgtccc gtggagcttg tggcggatat
      961 ttgatattcc cgcttaatca aacgccgctc cgtagtcgtt tataattccg gcaagctcgc
     1021 tcggcgagcc tatcacgttt gtgagtgcct ccggcagcac gccgactata acggtttccg
     1081 caacaacggc ggacgccgtt acggacaagc cgtcagtctt tccgagcata acaagcctgc
     1141 cacttgtctc tatattgaaa acaatgcgat gtattacctg gttaagcccg gcctctctga
     1201 attcatttga aaatgccaca accgtaccgg aggtcagctg agcattaaat ttcacgcgcg
     1261 ggccgtagcc gtttgtatat tcacttccca aaaacgttcc gattggtata gatatgctga
     1321 atgtttcgtt ttctgcggcg atttcggata cggcaaggga aatattgctt tgaagtaaat
     1381 ttattttaac tgtatcaacg tggagagaag ttacatttcc ctccgcgttt ctttctgttt
     1441 taactatatc gttatatgct gtttccgatt cggcgaggac ctcggcaacc gctttgtttg
     1501 tcgagtttaa aagtattctt tcggctactg tctcgctgta cctcgtaatg acggggcgca
     1561 tacgacaggt aaaggtgaaa acaagcaccg aaacgctcat aacgaacaca gcaaaagaga
     1621 agaacaccct ttttgcacag tttttccttt tcattacttt atctgcctcc cctgtttcta
     1681 ttctacgcag agaaggcatt gtacgttaaa acgccataaa aaaggagctg taaaaagctt
     1741 tacagccctc aaaaaaagta tcttttttct gtattcagcc gaaaatcatc tctgttattt
     1801 tttcatctat cgcatttaga aattctctgc cgttttttgc ctctttcgcg acgctctcaa
     1861 gtatctgctt ttcggtataa acggaatccg atactccgcg agtgagctga gacgttctgt
     1921 ttgccgactc agacactccc gcaaacagcg caaaggcaca caaaacaact gtcagtaccg
     1981 cgagatattt tttaattatc ctgtcttttt tcttcatttt aatacggcca ttttgccgca
     2041 acgggtcacc ttccgtatac ggcacaatgc tcctttcgtt tatttaatgg ttcagacgtt
     2101 ggaatttata aattcgacaa tagcttttcc cataaggtgc ccggaatatt ccgcctcggc
     2161 tattgtattt gaatcgctgc cgaactcaag cagcaccgag cacggcgtta cgtccatatt
     2221 gtattttctt gcggaaaaca atatcggccg cataagcccc ggatacatgg tttcggctgt
     2281 ctgctgcagc tttagcgcga atgtaagatt ttgctcccat gtcggaaacg acgtgacctt
     2341 gccgtcctca cagcctgcaa ttatcattat ctgcgccgcc tttctgccgt ttaccgtggt
     2401 tgtcggcttt atgcgcgtac cgtcgctttg ctcaatcgcg tctcggtgaa cgtcaagaac
     2461 tatctttacg gacggatttt cttccattat tttaagtatt gtctcacgcg agcggtcata
     2521 tgcaccggta tacgctctgt cgtgtatctc ggtgtcatgt attacgccta ttcccgcctt
     2581 ttgaagttcc tccgaaacgg cgtcgcccac gcgcaccata tttcgcgacg agtcatcgct
     2641 cctcagttca aaatcgcgcg tataccagcc cttatccata agctgatagc actctgtggt
     2701 atgggtgtga aaaataagaa ccgttggcgc gctcttatcg gcaatatcga gattacactc
     2761 gccgtttaac gagctttcga tatttatgct gtgcgacggc gttgtgtttc gtaccgatat
     2821 atttttatat gtcgatgttg cggacgacgc atcgaatttc ttttcgacta tggtgccgtc
     2881 atgatttgcc gaggcgtatt gtttttcggc gtcagccatc attttaacta tatcgcgagg
     2941 aacagtcgga gtgcttccgg cgacgctttc gctctgctcg tttttttcgc tcggcaatga
     3001 aattacggcg ttttgctgtg agctttgcgc ggaaacgctt ttttcgattg accttaccgc
     3061 actgcttttt gcagacagaa cggcaccctc gggcagcatc agtcccgcgc ctataacggc
     3121 ggttctgccg acggcatttc cgaaacggct tccgcacaca aatgccaata ttatgattat
     3181 cggcagggcg acaagcacct catttctttt tttcggaatt ttacccgtat gtcttttttg
     3241 tttcttcatt tgatttacat agcctttccg gccacaattt gttttttcgc aagtcatacg
     3301 ccgtatgttg cagccgtata cggcgtataa caaaaggctt aggacgatac ggcaatatca
     3361 cacagctgcg catatgacct ttgtaagatt atatgcgcgt ttttccgatg ttatacgaga
     3421 gaaagaagag ttttcatatc catatcgggt tgcagagcgg aatttatcgc aagagccaaa
     3481 aggcgtgacg cacggcttat cataacgtcg atttcgcgcg gtgtcaccat cattttttcg
     3541 gctctttcgc gttccggctg cggtatattt tcatttcccg ttatatcaaa gacaagtgcg
     3601 gcggcatcta ccaccgtcgg cacccctatc gctattaccg gcactccgag ggtctttcgg
     3661 cttatctcgg cgcgatgatt gccgactccc gaacccggaa ctataccggt atttgtcatc
     3721 tgcaccgtac agccgagcct ttttatgctg cgtgccgaca gcgcgtccac ggttattaca
     3781 gcgtcggggg aagtctcatt cattattccg cgaatcatct cggcgctttc tataccggtc
     3841 tgaccgagca ctcccggaga agcggacgat acaggccgaa gagacggaag gcctatctcc
     3901 tccgccagag attttgtgat atggcgcgtt gaaaatatat cttttgcggt cttgggaccg
     3961 agagcgtcgg gtgtaatatc cgaattgccg agccccacca caagaactcc cgaggtgttt
     4021 ttcggcaaca gctttt
//
LOCUS       DBMDVO010000039         4030 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9624_length_4030_cov_1.206159, whole genome shotgun sequence.
ACCESSION   DBMDVO010000039 DBMDVO010000000
VERSION     DBMDVO010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_4030_cov_1.206159"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="ACZPE2_01050"
     CDS             <1..368
                     /locus_tag="ACZPE2_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1318464.1"
                     /translation="LTPTEIYVKPVLVLAKEVKIKAVSHITGGGFYENIPRSLPEGIT
                     AVIEKKALKILPVFDIIEKTGNIPERDMFNTFNMGVGMSITVAPEDADRAVRILRESG
                     TDAYIIGETASGEDGVKIC"
     gene            362..994
                     /gene="purN"
                     /locus_tag="ACZPE2_01055"
     CDS             362..994
                     /gene="purN"
                     /locus_tag="ACZPE2_01055"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1318465.1"
                     /translation="MLKTKIAVFVSGGGTNFEALINAQENGKIPHGEIVLMVSSSKTA
                     YALKRAENHGIKSVVCSKKELGSQESFEKEILKNLEENNIQLIVLAGFMSILSKEFTK
                     KYENRIINIHPSLIPSFCGEGFYGLHVHEAALKKGVKVTGATVHIVNEIPDGGPIIMQ
                     RAVYVEENDTPEILQKRVMEQAEWKILPESVELMCSGKVKIENGRTYCNE"
     gene            987..1730
                     /locus_tag="ACZPE2_01060"
     CDS             987..1730
                     /locus_tag="ACZPE2_01060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1318466.1"
                     /translation="MNENKFTGKAELYSKYRPSYPEKLIDWLYDMTNAVAVADIGAGT
                     GIFTSALLKKPWSVTAVEPNSDMLSVLKKALGGKVKTVVASAESTTLNAKSINLITVA
                     QAFHWFDKARFKAECRRILTHDGHLAIVWNSRCQNSLEEERNKICMKYCDCYRSGHVK
                     TGYDDFDGDSFLRNEYFKNTDFLRLENKRFVTKEQFIGDNLSRSYAPRRDDSGYDGFV
                     CELENAFSRHEKSGKVSQNYITECYLGSF"
     gene            1755..2471
                     /locus_tag="ACZPE2_01065"
     CDS             1755..2471
                     /locus_tag="ACZPE2_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1318467.1"
                     /translation="MEIKSIKEELNSLAYHGRGIMIGKSADGKKAVIAYFIMGRSENS
                     RNRVFVEDGEGIRTQAFDESKMVDPHLIIYAPVRVLGNKTIVTNGDQTDTIYELMDKQ
                     MTFEQSLRTREFEDDAPNFTPRISGIIHIDNGEMNYAMSILKSADGDGSSCQRYTYAY
                     QNPLCGKAKFIHTYKCDGNPLPSFEGEPKTLELPDTDIDTLTKLIWENLNEDNKVSLF
                     VRFIDIETNEYTQRIINKNN"
     gene            2496..3674
                     /locus_tag="ACZPE2_01070"
     CDS             2496..3674
                     /locus_tag="ACZPE2_01070"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1318468.1"
                     /translation="MKEIELKYGCNPNQKPSKIFMADGRDLPVTVLNGRPGYINFLDA
                     LNSYQLVKELESAIGLPAAASFKHVSPAGAAVGLPLSDTLRKIYWVDDDAVLSPLASA
                     YARARGTDRMSSFGDWIALSDICDVPTALLIKHEVSDGIIAPGYEKEALEILKSKKKG
                     NYNIVEIDPEYKPAPIEHKDVYGVTFEQGRNELKIDESSLLNIVTESKELPDSAKRDL
                     IISLITLKYTQSNSVCYAKDGQTIGVGAGQQSRIHCTRLAGSKADNWHLRQHPKVLAL
                     DFIKSIGRPNRDNAIDVYISDDYEDVIGDDVWQNTFNTRPEPLTKEEKKAWLAQFHDV
                     SLGSDAFFPFDDNIERARRSGVSYIVEPGGSIRDDIVIDCCNKHGITMAFTGIRLFHH
                     "
     gene            3698..>4030
                     /locus_tag="ACZPE2_01075"
     CDS             3698..>4030
                     /locus_tag="ACZPE2_01075"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083397.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1318469.1"
                     /translation="MKILVVGAGGREHAIIKKLKENPEVTEIFACPGNGGIAKDAECV
                     NISATDLDGITDFAVKMNIDFCVVAPDDPLVLGLVDILESKGIPCFGPSKKAAIIEGS
                     KVFSKNLMK"
BASE COUNT         1248 a          876 c          949 g          957 t
ORIGIN      
        1 ttttaactcc caccgagata tatgtaaagc ccgtgcttgt gctcgcaaag gaagttaaaa
       61 ttaaggctgt atcgcacata accggcggcg gcttttatga gaatattccg agaagcctgc
      121 ccgaaggcat aaccgctgtt atcgagaaaa aagctcttaa aatactccct gtattcgata
      181 ttatcgaaaa aacaggcaac attccggaaa gggatatgtt caacaccttt aatatgggcg
      241 tgggaatgag cataacagtt gcccccgagg acgccgacag ggctgtaaga atccttcgcg
      301 aaagcggcac agatgcatat atcataggtg aaaccgcaag cggcgaagac ggtgttaaaa
      361 tatgctgaag acaaaaatag ccgtctttgt ttccggcggc ggaacaaatt ttgaggctct
      421 tataaacgct caggaaaacg gtaagatacc gcatggcgag atagttttga tggtgtcctc
      481 ctcaaagact gcatacgcgc tcaaaagagc cgaaaatcac ggtataaaat ccgttgtatg
      541 ctcaaaaaaa gagctcggaa gccaggaaag ctttgaaaaa gagattttaa aaaatctcga
      601 agaaaacaat attcagttaa ttgttcttgc gggctttatg tcaatactct caaaggagtt
      661 cacaaaaaag tacgaaaacc gaatcataaa cattcacccg tcgcttatac cgtcgttttg
      721 cggtgagggc ttctatgggc ttcatgtaca cgaagcagct ctgaaaaagg gtgttaaggt
      781 tacgggcgct acggtgcata tagtaaacga gattcccgac ggcggaccga ttattatgca
      841 aagggcagtt tatgttgagg aaaatgacac tcccgagata cttcaaaaac gcgttatgga
      901 gcaggcggag tggaaaattc tccccgagtc ggtcgagctt atgtgctcag gtaaagtaaa
      961 gatcgaaaac ggcaggacat actgcaatga atgagaacaa attcacgggt aaggcggagc
     1021 tttattcgaa gtatcgtccg tcatatcccg aaaagcttat tgattggctt tacgatatga
     1081 ctaacgccgt agccgtggcg gatataggcg caggcacagg cattttcaca tctgcgctgc
     1141 tcaaaaagcc gtggagcgta acggcggttg aacctaactc ggatatgctc tcggttctca
     1201 aaaaagcttt gggcggcaag gtaaaaacgg ttgttgcctc agcggaaagc acaacgctta
     1261 acgccaaaag tattaacctg ataaccgttg cccaagcgtt tcattggttc gataaggcaa
     1321 ggttcaaggc ggagtgccga cggatattaa ctcatgacgg acaccttgct atcgtgtgga
     1381 attcaagatg tcagaacagt cttgaagaag agcgcaacaa aatctgtatg aaatattgcg
     1441 actgctaccg aagcggtcat gtcaagacgg gttatgatga ctttgacggc gactcatttt
     1501 taagaaacga atattttaaa aacaccgatt ttttaagact tgaaaataag agatttgtca
     1561 caaaggaaca gtttatcggc gacaatcttt cccgttcgta tgcgccgcgg cgtgatgaca
     1621 gcggctatga cggctttgtt tgtgaacttg aaaatgcttt ttcaagacat gaaaaaagcg
     1681 gcaaggtttc gcaaaattat attaccgagt gttatctcgg ttcattttaa taaattttga
     1741 aaggatattg cttcatggag ataaaatcta ttaaggaaga gcttaattct ctcgcatatc
     1801 acggtagggg aattatgata ggaaagagcg cggacggcaa aaaggctgtt atagcttatt
     1861 tcattatggg cagaagcgag aacagcagga accgtgtgtt cgttgaggac ggcgaaggta
     1921 ttcgcactca ggctttcgac gagtccaaga tggttgaccc ccaccttatc atctacgctc
     1981 ccgtaagagt tctcggtaac aaaactattg ttacaaacgg cgaccagacc gatacaattt
     2041 atgagcttat ggacaagcag atgacctttg agcagtcgct tcgcacaaga gagtttgagg
     2101 atgacgctcc taatttcacg ccgagaatat cgggcattat ccacatagac aacggcgaaa
     2161 tgaactacgc catgtcaatt cttaaatcgg cagacggcga cggcagctct tgtcagagat
     2221 acacctatgc gtatcaaaat ccactctgcg gtaaagcaaa atttatccac acttataaat
     2281 gcgacggaaa tccgcttccg agctttgaag gtgagccgaa aacgcttgag cttcccgaca
     2341 cggatattga tactcttaca aagcttatct gggaaaatct caacgaggac aataaggttt
     2401 cgcttttcgt aagatttatt gacattgaaa caaacgaata cacccaaaga atcatcaaca
     2461 aaaacaatta agaagtgact ttacggaggt ctgaaatgaa agagatcgaa cttaaatacg
     2521 gctgtaaccc aaaccaaaag ccgtctaaaa tttttatggc ggacggcaga gacttaccgg
     2581 ttaccgtttt aaacggcaga cccggctata ttaacttcct cgacgcgctg aacagctatc
     2641 agcttgtaaa agaattggaa tccgccatcg gtcttcctgc ggctgcttca tttaagcatg
     2701 tcagccctgc aggcgcggct gtcggcttgc cgctctccga tacgctcaga aaaatttact
     2761 gggttgacga cgatgcggtt ctctcccctc tcgcatcagc ttatgcaaga gcaagaggca
     2821 cagacagaat gtcgtccttc ggcgattgga tagcgctctc agacatatgt gacgttccca
     2881 cggcactgct tataaagcat gaggtttccg acggtataat tgctcccgga tacgaaaaag
     2941 aggctcttga aatactcaaa tccaagaaaa agggaaatta caatatagtt gaaatagacc
     3001 ccgaatacaa gcccgctccg atagaacata aggacgttta cggcgttaca tttgagcagg
     3061 gcagaaacga attaaaaatt gacgaaagct cacttttaaa catagtcacc gaaagtaagg
     3121 aacttccgga tagtgcaaag cgcgacctta taatttcgct tatcacgctc aaatacacac
     3181 agtccaactc cgtatgctat gcaaaagacg gtcagaccat aggcgtgggc gccggtcagc
     3241 agtcaagaat tcactgcaca cgcttagccg gcagcaaagc cgacaattgg catttaagac
     3301 agcatcctaa ggttctcgct cttgacttta taaagtccat cggcagaccc aacagagata
     3361 acgccattga tgtatacatc tcagacgact atgaggacgt aatcggcgat gatgtttggc
     3421 agaacacctt taacactcgc cccgaacccc tcacaaagga agaaaaaaag gcttggctcg
     3481 cacagtttca cgatgtatcg ctcggctcgg acgcattttt cccgtttgac gacaacatag
     3541 agcgtgcaag aagaagcggt gtttcgtata tagttgaacc gggcggctcc atacgtgacg
     3601 acatcgtaat tgactgctgc aacaagcacg gcattaccat ggcattcaca ggaatacgtc
     3661 ttttccatca ctgataaaac aataagaggt ttctaaaatg aaaatacttg tagtcggcgc
     3721 gggcggaaga gaacacgcca taattaaaaa gctgaaggaa aatccggagg tcaccgagat
     3781 ttttgcgtgc cccggaaacg gcggcattgc aaaagacgcg gaatgcgtta acataagtgc
     3841 cacggattta gacggcatta cggattttgc cgttaaaatg aacattgatt tctgcgtagt
     3901 ggctcctgat gacccgctcg tattaggatt ggtggatatt ctcgaaagca agggaatacc
     3961 gtgcttcggt ccgtctaaaa aagcggccat aatcgaggga agcaaggttt tctcaaaaaa
     4021 tcttatgaaa
//
LOCUS       DBMDVO010000040         4013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9685_length_4013_cov_1.208078, whole genome shotgun sequence.
ACCESSION   DBMDVO010000040 DBMDVO010000000
VERSION     DBMDVO010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_4013_cov_1.208078"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(138..1136)
                     /locus_tag="ACZPE2_01080"
     CDS             complement(138..1136)
                     /locus_tag="ACZPE2_01080"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HIR1318470.1"
                     /translation="MSKWYIGAGEQNDIVISTRIRLARNIEDYPFPCKLDTKTRTALN
                     KKIGDAVCGRENLEFIEMKTLTQPQIVSLAEKHLISPEFASSYDGRALLLSDSEGISI
                     MLCEEDHIKIQVMKPGLSLETAYAEADRIDDELNEKFVFAFDDRLGYLTQCPTNLGTG
                     MRASVLLHLPALTACGQMSSISSTVSKLGLTVCGSYGEGSVPMGDIYCLSNQVTLGIS
                     EKAAIDNLKTVTLQLAAQERTARAEMVKSIETEDEIFRAYGILKSARILPVKEFMTLV
                     SKVRLGAVAGFINVKPETVDELMISMQPATINAAAGKNLDSRERDIERAKQVRQRL"
     gene            complement(1133..>1450)
                     /locus_tag="ACZPE2_01085"
     CDS             complement(1133..>1450)
                     /locus_tag="ACZPE2_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR1318471.1"
                     /translation="GALSNGIVRCEKCGSTFNDIVRSGRMGCGDCYSTFYDKLLPSFE
                     RIHGRTHHEGKKANTSPEIKKLSKTEELKENLKQAVEAQNYEEAARLRDEIKKLESED
                     NQK"
     assembly_gap    1451..1460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1646..2095)
                     /locus_tag="ACZPE2_01090"
     CDS             complement(1646..2095)
                     /locus_tag="ACZPE2_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIR1318472.1"
                     /translation="MNLSKEIAGMILDMLSDSGTTEIRRNDLAEQLGCVPSQINYVIS
                     SRFTPEHGYIVESRRGGGGYIKITRATYDLSTLKMHLINSIGNNIDERSCRAHIVNLH
                     DRGLLEDKEAAVMLAATADKSYHDVPHELRDKLRANIFKQMLISSMK"
     gene            2600..2761
                     /locus_tag="ACZPE2_01095"
     CDS             2600..2761
                     /locus_tag="ACZPE2_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318473.1"
                     /translation="MNCFGGCGNNGLLILVIIIILLFADGSYGKNGCGCGNDCGCGNS
                     GCGGCGCGC"
     gene            complement(2926..3888)
                     /locus_tag="ACZPE2_01100"
     CDS             complement(2926..3888)
                     /locus_tag="ACZPE2_01100"
                     /inference="COORDINATES: protein motif:HMM:NF020442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HIR1318474.1"
                     /translation="MKIEDINLPQGLMGTRIKDRLEDAFDVNFDVSEIKIDTFDDFGR
                     VLLEPYNGGEKLYYRGERIKSRTRRLIPTLLRNEDVIAKMNGGSIFNIDSKKLFDFYC
                     SRKHFISVYKTLYGEPSENSMYYMTAFAQHYLDISPFVDFTKCIYVALSFAIKGRETA
                     DSDIVIYTAEDIGDDDTSSDMNEVNEWLGNYNVNIVNTDFREETIKYFTEKRKNRGFM
                     PKLSLREDFKKLEELCSSMSPVAKLIDIPTNDLMKYQQGVFLLLNNFSLVDSMYLTKS
                     VRRGFVIKKYIINGALCGELKKYLAANAPQYRYKCLMNIAEAVK"
BASE COUNT         1003 a          969 c          808 g         1223 t
ORIGIN      
        1 atgtcccaaa tcctcggctg cctttaccgc cgcattcaag gacttatttg ctttttccgt
       61 aaaaccggtg aatttgaaca taactgtcat tcctttcaaa tcaattgata aattaacgcc
      121 gcaaagcgaa aattcattta aagtctttgt cttacttgct ttgcacgctc aatgtcgcgc
      181 tcgcggctgt caagattttt gcccgccgcc gcgtttattg ttgcgggctg cattgaaatc
      241 ataagctcat cgacggtctc gggcttaaca tttataaaac ccgcaaccgc tccgagacga
      301 acctttgata caagagtcat aaattccttg acggggagaa tcctcgccga tttcagaatt
      361 ccgtaggctc tgaatatctc atcctcggtt tctatgcttt taaccatttc cgctctcgct
      421 gttctctcct gcgccgcaag ctgcaacgtt accgttttga gattgtcaat agcggctttt
      481 tcggagatgc cgagagttac ctgattactg aggcagtata tatcgcccat gggaacgctt
      541 ccctcaccgt aagagccgca caccgtaaga ccgagctttg atacggttga agagatagaa
      601 ctcatctggc cgcacgccgt aagcgccgga agatgaagga gcaccgaggc tctcataccc
      661 gtaccgagat tggtcggaca ttgggtaagg tagccgagac ggtcatcaaa tgcgaaaaca
      721 aatttttcgt taagctcatc gtctattctg tctgcctcgg cataagccgt ttcgagtgaa
      781 agacctggtt tcataacctg tattttaata tggtcctctt cacagagcat tatgcttatg
      841 ccctcgctgt ctgataaaag caacgctctg ccgtcataag acgatgcaaa ttcgggactt
      901 atcagatgct tttcggcaag cgatactatc tgcggttgag taagagtttt catctcgata
      961 aattcgagat tctctctgcc gcatacagcg tcgccgattt ttttattcag agccgttctc
     1021 gttttcgtat caagcttgca gggaaaagga taatcctcaa tatttctcgc aagtcttatg
     1081 cgtgtgctta taacgatgtc gttttgttcg cctgcgccta tataccactt actcattttt
     1141 gattatcctc gctttccagc tttttgatct cgtcgcgaag gcgtgccgcc tcttcgtaat
     1201 tctgagcctc aaccgcctgc ttaagatttt ccttaagctc ttcggttttg ctgagcttct
     1261 ttatttccgg agaagtattt gcctttttac cctcgtgatg ggttctgccg tggatacgct
     1321 caaatgaggg aagaagctta tcataaaatg tactgtaaca atcgccgcag cccattctgc
     1381 cgcttcttac gatgtcatta aacgtactgc cgcacttttc acagcggact ataccgttgc
     1441 tgagagcccc nnnnnnnnnn ttgaaaaacg agccgagcat atctgaaaag ttgaagccga
     1501 aatccggaaa gacgctgtca tagccgagag cgcgtgcaca atcggagcaa agatgagctt
     1561 ccgtggcctc gccgtttaca atccttttga tatgtgtagt tgcctcatat ttaccacagt
     1621 tttgacacaa catataaatc actccttatt tcatagatga aatcaacatt tgcttaaata
     1681 tattcgccct aagcttatcg cgaagctcgt gcggcacatc gtgatacgac ttatcggcgg
     1741 ttgccgccag cattaccgcc gcctctttgt cctccaaaag ccctctgtcg tgcaaattca
     1801 cgatatgtgc gcgacaggat ctttcatcaa tattatttcc tatcgaattg atgaggtgca
     1861 ttttcagagt gctgagatcg tatgtggctc tggttatctt gatataacct ccgccgccgc
     1921 ggcggctttc tactatatag ccgtgctccg gcgtaaaccg tgacgaaatg acgtaattta
     1981 tttggctcgg tacgcaccct agctgctctg ccaaatcatt gcggcgtatt tcggtagttc
     2041 cgctgtcgga gagcatatca agaatcatac cggctatttc cttgcttaag ttcatttgtt
     2101 aatcactccg tcttttccga ttgtcagagg acttttacag tacccggctc atcgcctaat
     2161 ctatttgact ttgactatct ttgacctttg acattttcta ttatatgttt ttcacccttt
     2221 ttgtcaaagg tcaaaaaaag tcaaattcag ttttagtgaa gatgaaaata catattatct
     2281 cttgttttct ttaagttact gcttttttta tgctttttgc tcatttttca ttccgaggct
     2341 gactctttgg ctcataattc aggcaaaaac gagcccaaaa gccctctgta ccgtcaattt
     2401 cacgcctccc ctcctcctgt ttcgcgaaag tttaaaggtc aaattttttc tcaaaggtca
     2461 aatttttttg acttttatac tcacaaaaat atattgtatg ccgaaacatc gaaaaatatt
     2521 aacacaagct ttgccgttta cataatatgt attgaaggcg ggcaaaaacc tgccgttaaa
     2581 tctgaaagga ggatattgga tgaactgctt tggcggatgc ggcaacaacg gtcttttgat
     2641 acttgtcatc atcataatcc tgctctttgc ggacggtagc tacggcaaga acggctgcgg
     2701 ctgcggtaat gactgcggtt gcggtaactc aggctgcggc ggctgcgggt gcggctgcta
     2761 atcggtaaaa cggcacagcc gaagattcgg catacgcggc cgcttacgcg caaaaatccg
     2821 accgtataca agccgaatct tcaccgataa aaaatccgcc tcgggaatcc tcccggggca
     2881 gatttgtttt taattgcaca atatacagtt ttttcacaaa aaccgctatt taacggcttc
     2941 ggctatattc ataaggcact tgtaccgata ctgcggggcg tttgccgcaa ggtatttttt
     3001 aagctctccg cacagcgcac cgtttattat atacttctta ataacgaaac cgcggcgcac
     3061 ggattttgtc agatacatag aatctacaag gctgaagttg tttagcaaga ggaaaacgcc
     3121 ctgttgatat ttcataaggt cgtttgtggg tatgtcaatc agctttgcta cgggcgacat
     3181 actgctgcaa agctcctcaa gctttttaaa atcctccctc agggaaagct tcggcataaa
     3241 ccccctgttt ttcctctttt ccgtaaaata ttttatcgtc tcttcgcgga aatcggtgtt
     3301 tactatgtta acattataat ttccgagcca ctcattgacc tcgttcatat ctgacgaggt
     3361 atcatcgtcg cctatgtcct cggctgtgta tataactata tcgctgtccg ccgtttctct
     3421 gcccttaatg gcaaacgaga gggctacgta aatacatttt gtgaagtcca caaacgggct
     3481 tatgtcaagg taatgctgcg caaatgccgt catatagtac atactgttct ccgacggttc
     3541 gccgtagaga gttttgtata ccgatataaa atgttttctt gaacagtaaa aatcaaaaag
     3601 ctttttactg tcaatattaa aaatcgaacc gccgttcatt ttcgctatca cgtcttcatt
     3661 tctcagaaga gttggtatga gccttctcgt gcggctcttt attctctcgc cgcggtaata
     3721 gagcttttcg ccgccgttgt atggctccag cagcactctg ccgaaatcgt cgaatgtatc
     3781 tattttaatt tcggacacat caaaattgac atcgaacgca tcctctaatc tgtcttttat
     3841 tctcgtcccc attaaaccct gaggcaaatt tatgtcttct atcttcaaag cagcatcacc
     3901 gtaccccgac ttttgttaca gtataacata aagcacagtg ttttggcaaa taaagataac
     3961 ctacgacaat atttgtgtta ttacacacac agcacaaaat ttgcacaaat aaa
//
LOCUS       DBMDVO010000041         3991 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9751_length_3991_cov_1.467866, whole genome shotgun sequence.
ACCESSION   DBMDVO010000041 DBMDVO010000000
VERSION     DBMDVO010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9751_length_3991_cov_1.467866"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="ACZPE2_01105"
     CDS             <1..467
                     /locus_tag="ACZPE2_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HIR1318475.1"
                     /translation="NGYLTSGDITFPSYGDHFGHITVEGTDIDTDVIFGDDTSLLKKG
                     ACMSYYSHIPGCGRGVLIGAHNNTYFHSLQDVQTGALVNLSTTYGNFVYRVYLTNVIN
                     VDTDSSYRAALNGDKETLILYTCYPNNTLSLTPYRFIAYCEKVSGPHVNMYE"
     gene            481..1344
                     /locus_tag="ACZPE2_01110"
     CDS             481..1344
                     /locus_tag="ACZPE2_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318476.1"
                     /translation="MTKNSKAMRRVLCGVVSFLLSFFSCLFVMCLVFKFTVLNSSFLV
                     GVEKRSDYAEALHSELKEQFVSYGSAGNVDESFFDSVFENIITPDRIDEDTKAVITDF
                     YNGEVKDSIDTSDIQSELETRLLEYAAEKGFAVDDGLKNNIKDMAVQFGDLYNSYVSL
                     FSNSYFKSAGNMLKRYNPYADYAAIIAVTLSLIAGLVLRMSYKKRKNVYRYYIYAFSG
                     TALMLLAAPLAAIIGGVGTRINIGTKSLYSFASGFMTDTLWAFVFAAAAVAVVTAAFY
                     ALRRIFVKKNI"
     gene            complement(1403..1564)
                     /locus_tag="ACZPE2_01115"
     CDS             complement(1403..1564)
                     /locus_tag="ACZPE2_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318477.1"
                     /translation="MDMNIIKRDIGLMCAAQKLLNFFCIMKTVLTVATIAFSVLESVN
                     MLKNLKIRA"
     gene            complement(1581..2600)
                     /gene="spoVAD"
                     /locus_tag="ACZPE2_01120"
     CDS             complement(1581..2600)
                     /gene="spoVAD"
                     /locus_tag="ACZPE2_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1318478.1"
                     /translation="MAERLGKYTIILNSHPSVTGFAAVVGKTEGEGPLKDEFDFVFED
                     DTLGEASFEKAESALQKEAVTRALSKAGKTPDEVDFSLAGDLLNQCIGSSFGLRSLNM
                     PFIGLYGACSTMALSLGLASMLVDCGAARVTAASTSSHFCSAERQFRLPLEYGGQRTP
                     TSQRTATAAGASVVEKKQSGKPAVDAVTIGRITDLGIKDANNMGAAMAPAAANTIADF
                     LKDTSSSPSDFDMILTGDLGKVGSRLLCELLQKDRGIDIESVHADCGLLLYDAEKQDV
                     HAGGSGCGCGASVLNSYIMRRLEGGELSRVLFVATGALMSPTSSLQGESIPSIAHAVL
                     LSKPT"
     gene            complement(2610..3068)
                     /gene="spoVAC"
                     /locus_tag="ACZPE2_01125"
     CDS             complement(2610..3068)
                     /gene="spoVAC"
                     /locus_tag="ACZPE2_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HIR1318479.1"
                     /translation="MKGLKYMTTGKEYGKMTKSASPPSPKIKNIINAFWTGGLICTIG
                     QALNTLYTEAGFSEDEVNAGVSVTLIVIAACLTGLGVYDKIAKVAGAGTIVPITGFAN
                     SIVSPAMEFSAEGKILGTGAKLFSLAGPVIAYGTLAAFVYGIICYCFKLY"
     gene            3171..3860
                     /locus_tag="ACZPE2_01130"
     CDS             3171..3860
                     /locus_tag="ACZPE2_01130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318480.1"
                     /translation="MALIEVKDLYKIYNPGENEVRALDGVSLTIDRGEFVAIIGHSGS
                     GKSTFMNMLGLLDVPTSGEYKLDGHNTSKLSDDEMSEIRNKEIGFIFQGFNLISSLTA
                     QENVELPLAYRGMPKAQRHVLSEDALKRVGLEKRMNHLPKQMSGGQQQRVAIARAVAA
                     KPPIILADEPTGNLDSHSGAEVMRILHELHREGRTVILITHDNEIAANAHRVIRIQDG
                     QVVEDYINADK"
BASE COUNT         1002 a          964 c          991 g         1034 t
ORIGIN      
        1 ataacggtta tcttacatcg ggcgatataa catttccgtc ttacggtgac catttcggtc
       61 atataactgt tgaggggacg gatattgaca cggatgttat tttcggcgac gatacctcgc
      121 tgcttaaaaa aggtgcgtgt atgtcgtatt acagccatat tccgggctgc ggcagaggcg
      181 tgcttatagg tgcccacaac aatacttatt ttcactcgct tcaagatgtg cagacgggtg
      241 cgcttgtaaa tttgagtacc acctacggta actttgttta ccgcgtatac ctcacaaacg
      301 taataaacgt agacacagac agctcttacc gcgccgcgct caacggcgac aaggagacgc
      361 tgatactata tacctgttac cccaacaaca ctttatcgct cactccgtac cgctttatag
      421 cgtactgcga aaaggtgtcg gggccgcacg tgaatatgta cgaatgagga ggcgcgtgat
      481 atgactaaaa attcaaaagc catgcggcgc gtgctctgcg gagtggtttc gtttctgctc
      541 tcgttctttt cgtgcctttt tgtgatgtgc cttgtgttta aattcacggt gctcaattcc
      601 tcattccttg tcggggtcga gaagcgttca gactatgcgg aggctttgca ttccgagctt
      661 aaggagcagt tcgtctcata cggcagcgcg ggtaatgttg acgagagctt ttttgattcc
      721 gtgtttgaaa atatcatcac tcccgaccgt atcgacgagg acacaaaagc cgtaattacc
      781 gatttttata acggcgaggt caaggactcg attgatacct ccgatataca aagcgagctt
      841 gaaacaagac tcttggaata cgccgccgaa aagggctttg cggtggacga cgggctcaaa
      901 aacaatataa aggatatggc ggtgcaattc ggagacctct ataattccta tgtatcgctg
      961 ttttcaaatt cgtatttcaa atccgccggc aatatgctca aacgctacaa cccttatgcc
     1021 gactatgccg cgattatagc ggttacgctt tcgctgattg cggggctcgt gcttcgtatg
     1081 tcgtataaaa agcgcaaaaa cgtgtacaga tattatattt atgcgttttc gggtaccgcg
     1141 cttatgcttc ttgcggcacc gcttgccgcg attataggcg gagtaggcac aagaataaac
     1201 atcggtacaa aatcgcttta cagctttgcg agcggattta tgacggacac tctttgggcg
     1261 tttgtttttg ccgctgcggc cgttgcggtc gttaccgctg cattctatgc gctcagacgc
     1321 atatttgtaa aaaagaatat ttgacgaagt gccaaaaaaa taacccgtga gcggtaagat
     1381 taccgcccgc gggttttcgg ttttatgccc gaattttcag atttttgagc atatttaccg
     1441 attctaaaac ggaaaaggct attgtcgcaa cagtcagtac cgttttcatt atacagaaaa
     1501 aattaagaag cttttgcgcc gcgcacataa ggcctatatc gcgtttaatt atattcatat
     1561 ccatattggt tttattcctt tcacgtcggt ttagataaaa gaacggcatg ggctattgac
     1621 ggtatactct cgccctgcag tgaggaagta ggcgacatca gtgcgcccgt ggcgacaaag
     1681 agaacgcgcg acagctcgcc gccttcgagc cgccgcataa tatacgaatt taaaaccgag
     1741 gcaccgcagc cgcagccgga gccgcctgcg tgaacatcct gcttttcggc gtcgtacagg
     1801 agcaatccgc agtccgcgtg aacgctctct atatcgattc cgcggtcctt ttgcagaagc
     1861 tcgcacaaaa gccttgaccc gaccttgccg aggtcgcccg tcaatatcat atcaaagtcg
     1921 gacggcgaag aagaggtatc tttaagaaaa tcggctattg tatttgcagc ggcgggtgcc
     1981 atggcagctc ccatgttgtt tgcgtccttt atgccgagat cggttatcct gccgatggta
     2041 acggcgtcaa ccgccggctt gccgctttgc tttttttcta ctacgcttgc ccctgccgcc
     2101 gtggcggtgc gctgagaggt gggtgtgcgc tgtccgccgt attcgagcgg aagcctgaac
     2161 tgacgttccg ccgagcagaa gtggctcgat gtcgacgcgg ctgtgaccct tgccgcgccg
     2221 cagtcaacaa gcatcgaggc aagaccgaga gacagagcca tggtggagca cgctccgtaa
     2281 agccctataa aaggcatatt gagcgatcgc agaccgaacg acgagcctat gcattggttc
     2341 aaaaggtcgc cggcaagaga aaaatccact tcgtcgggag ttttcccggc ttttgataat
     2401 gcgcgcgtca cggcctcttt ttggagtgcg ctctccgctt tttcaaacga agcctcgccg
     2461 agcgtatcgt cctcaaaaac aaagtcgaac tcgtccttga gcggcccctc gccctcggtt
     2521 ttgccgacta cggccgcaaa gcccgttacc gaggggtgtg aattgagaat tatagtgtat
     2581 tttccgagtc tttcagccat aaactcacct caataaagct taaagcagta gcagattatt
     2641 ccgtaaacaa aagccgcaag tgtgccgtag gctataaccg gaccggcgag tgaaaacagc
     2701 tttgcacccg tgccgagtat tttgccctcg gcgctgaatt ccatggccgg cgatactatc
     2761 gaatttgcga agcccgttat cggcactatt gtgcccgcac cggcaacctt tgctattttg
     2821 tcataaacac cgagtcccgt aagacaggcg gctataacta taagcgttac cgaaactccg
     2881 gcgttcactt cgtcctcgga aaatcccgct tcggtataaa gggtgttaag agcctgaccg
     2941 atggtgcaaa taagtccgcc cgtccaaaag gcgttgatta tatttttgat tttcggagac
     3001 ggcggagatg cactttttgt cattttgccg tattccttgc ccgtagtcat atatttcagt
     3061 cctttcaaag ctatttttgc cgtcaaacgg gtaaaaattc ataatttata cattgattta
     3121 tattttttta tgttttaaaa tttaaacatc aaataaaggc ggtgctttta atggcgctta
     3181 tagaagtcaa agatttatac aagatataca atccgggcga aaacgaggtt cgcgcgcttg
     3241 acggtgtgtc gctgacgatt gaccgcggcg aatttgtcgc cattatcggc cattcgggtt
     3301 cgggtaaatc gacgtttatg aatatgctcg ggcttttgga cgttcccaca agcggcgaat
     3361 ataagcttga cgggcacaat acgtcaaagc tctccgacga cgagatgagc gagatacgaa
     3421 acaaagagat aggctttatt tttcagggct tcaatcttat atcgagcctt accgcacagg
     3481 aaaatgtgga actgccgctt gcgtaccgcg gtatgccgaa ggcacagcgt cacgttctgt
     3541 cggaggacgc gctcaagcgt gtcgggcttg aaaagcgaat gaaccatctt ccgaagcaga
     3601 tgtcgggcgg tcaacagcag agagttgcca tagcgcgcgc cgtagccgca aaaccgccca
     3661 taattttggc ggatgagccg accggcaacc tcgacagcca ctccggagcg gaggttatgc
     3721 gaatacttca cgagctgcac cgcgagggca gaacggttat actgattacc cacgacaacg
     3781 aaatagccgc aaatgctcac cgagtgatac gtatacagga cggtcaggtt gtagaggact
     3841 atataaacgc ggacaaataa accgaaaaga cacggtactg taagattagt cgcaaggcgt
     3901 tttttgttaa aacagctttt tacggctgtt tttaagcata atgtattgta tttttactcg
     3961 cattagtgta taataatttt gattatcaag g
//
LOCUS       DBMDVO010000042         3977 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9816_length_3977_cov_1.062951, whole genome shotgun sequence.
ACCESSION   DBMDVO010000042 DBMDVO010000000
VERSION     DBMDVO010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9816_length_3977_cov_1.062951"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="ACZPE2_01135"
     CDS             complement(<1..306)
                     /locus_tag="ACZPE2_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HIR1318481.1"
                     /translation="MNPVTIAIIAAVAAAVVFGVVGFVLGEVHRKKVAESAIGSATEE
                     ATRIVNQAVQTAENKKREAILEAKDEIHSLRSEAEKENRERRAEVQRQERRIIQKEES
                     "
     gene            complement(594..1706)
                     /locus_tag="ACZPE2_01140"
     CDS             complement(594..1706)
                     /locus_tag="ACZPE2_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1318482.1"
                     /translation="MKKIISVVLTLTLLAGILMLSGCSLKTEKLDTAALLGNYNVEEL
                     KGTEINVYNWGEYISDGEGGSVDVISEFEKLTGIKVNYNMYDSNEDLYAKLKSGGVNY
                     DVIIPSDYMAERLKNEGLLQKLDYSVITNYEGIGDEYKNLFFDENNEYTVAYTGGMVG
                     LIYNTTMVSEKPDSWSVMWDPKYSGEILTFNNSRDAFGVAQYLLGYDVNSTNPDEWRA
                     AEEKLKEQKPLIQSYVMDEVFNKMEAGEAAMAPYYAGDYLTMKQENDDLAFVYPKEGT
                     NKFVDVMCIPSSSENKKAAELFINYMCEPEVALHNANYLGYLCPVTAVLSDDRYVNKD
                     NEVLYPKQEVKTQFFHNLDQDTLDLLSKLWEDLKLA"
     gene            complement(1723..2544)
                     /locus_tag="ACZPE2_01145"
     CDS             complement(1723..2544)
                     /locus_tag="ACZPE2_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318483.1"
                     /translation="MKALKKIYIALIMLFLYAPMLVMVVFSFNATNSTSVMSGFSLKW
                     YVEMFRNATALTALRNTLVLAVTSALISIIIGTAAAVGINAMKKRWLRSAVMTVTNVP
                     MMNPEIVTGVSMMLLFVFAGKIVSSSSVLGFPTLLIAHVTFNLPYVILSVLPKLRQTD
                     RHLSEAAQDLGCTPTSAFFKVVLPNIKSGIITGFIMAFTLSLDDFIISYFVSGAKFQT
                     LPIVIFSMTKKKVKPDMYALSTLIFVSVLVLMILMNVLQARSEKKERKRAKGSEG"
     gene            complement(2541..3365)
                     /locus_tag="ACZPE2_01150"
     CDS             complement(2541..3365)
                     /locus_tag="ACZPE2_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318484.1"
                     /translation="MKKSSLTLKLINAPYMIWAVLFILAPLIMVIYYAFTDRSGAFTL
                     DNIKALSSYGAIFGRSAWYAVLATVICLILAYPLAYIMAKSRANTQRTIMMLIMLPMW
                     MNFLIRTYSWITILSNTGLINSLLKAVGLPTAQLINTPGAVILGMVYNFLPYMVLPIY
                     SVMSKLDKSLVEAAQDLGAGKLQVLKKVIFPLSLPGVISGITMVFVPSVSTFYISQKL
                     GGGKFLLIGDSIEMQFQSAYNYNLGASLSLVLMLLIIVCMLIMNRFADNEEGAMII"
     gene            complement(3369..>3977)
                     /locus_tag="ACZPE2_01155"
     CDS             complement(3369..>3977)
                     /locus_tag="ACZPE2_01155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318485.1"
                     /translation="IQQRLGITFIYVTHDQEEALSMSDTIVVMDGGVIQQIGSPTDIY
                     NEPQNAFVADFIGESNIVDGVMLSDYNVEFQGMRCRCVDKGFGLNEPVDVVVRPEDID
                     MVPPGEGMLSGDVTSVTFKGVHYEIIVDIKGFKWMIQTTEKAEVGDRIGISIKPDEIQ
                     IMKKSEYSGLYGDYSSFSEEFDELSDPNAELTDEDEVSEIGE"
BASE COUNT         1072 a          961 c          851 g         1093 t
ORIGIN      
        1 agactcctct ttttgaatta ttctgcgttc ctgccgctga acctccgctc tgcgctctct
       61 gttttccttt tcagcctcgg atctcaacga atggatttcg tcctttgctt ccaaaatcgc
      121 ttctctcttt ttattttccg ctgtctgaac ggcctgattt acgattctgg tagcttcctc
      181 cgtagccgag cctatggcag actccgccac ctttttacgg tgtacctcgc cgagtacaaa
      241 tccgacgact ccgaaaacaa cagcagctgc gactgcggct attatagcta tcgttacagg
      301 attcaaaaag ttctgcacct ccttttggtt tttagttttc cgattatcat gcttgaaaat
      361 aagaaaattt attggttttt gacagtgtaa cggtatcatt tgtaatactg ttcgcatttt
      421 aatacactat ctaagatatt ttatatcttt ttatataata agtcaagaaa aagcggaccg
      481 ttaggcacaa aaactgtcga tatataagat acaaaagacg cgccgcaaag gctcacagcc
      541 gttgcgacgc gccaaatacg atttaaaatt tttaacgtat agccgaaacc gaattacgcg
      601 agcttgagat cctcccaaag ctttgaaaga agatcaagcg tatcctggtc aaggttgtgg
      661 aagaactgcg ttttcacttc ctgcttgggg tagagcacct cgttatcctt attcacatac
      721 ctgtcatcgc tgagaacagc cgtaacgggg cagagatagc caagataatt ggcgttgtgg
      781 agtgccacct cgggctcaca catataattt ataaacagct cagcagcctt tttgttttca
      841 gagcttgacg ggatgcacat tacgtcaaca aatttatttg ttccctcctt ggggtaaaca
      901 aatgcaagat cgtcattttc ctgcttcatt gtgaggtaat ctcctgcgta atacggagcc
      961 atggcggcct cgcccgcctc catcttatta aatacctcat ccataacata cgactgaatc
     1021 agcggctttt gctccttgag cttttcctcc gcagcgcgcc actcatcggg attcgtcgag
     1081 tttacatcgt agccgagtag atactgtgcc acgccgaatg cgtcgcgaga attattaaaa
     1141 gtaagaattt cgccggaata tttcggatcc cacataacgc tccagctgtc gggcttttcg
     1201 gaaaccatgg tcgtattata gataagcccg accataccgc ccgtgtaagc tacggtatac
     1261 tcgttgtttt cgtcaaagaa aaggtttttg tattcgtcgc cgataccctc ataattggtt
     1321 atcaccgaat aatcaagttt ctgtaagagc ccctcgttct ttaaacgctc tgccatataa
     1381 tccgacggaa ttataacatc gtaatttact ccgccgctct tgagctttgc ataaaggtcc
     1441 tcgttactgt cgtacatatt atagttgacc tttattcccg tgagcttttc aaattcggag
     1501 atgacgtcaa cgcttccgcc ctcgccgtcg gaaatgtact caccccagtt atatacattt
     1561 atttcggtac ctttgagctc ctcgacatta taatttccga gaagagcggc cgtatcaagc
     1621 ttttcggttt taagtgagca gccagaaagc ataagtattc ccgcaagcaa ggtcaatgtt
     1681 aatacaacag aaattatctt tttcattttt cacgttcctt tctcaaccct ctgagccttt
     1741 ggctctttta cgttccttct tttcgctgcg cgcctgaagc acattcataa gaatcataag
     1801 cacaagcacc gatacaaata tcagcgtcga taacgcatac atatcgggtt ttaccttctt
     1861 ctttgtcatt gagaaaatga ctatcggaag agtctggaat tttgcgccgc ttacaaaata
     1921 gctgattatg aagtcgtcca gcgaaagcgt aaaggccatt ataaagccgg ttattatacc
     1981 ggacttgata ttgggaagca caaccttgaa aaacgcagag gtcggtgtgc agccgaggtc
     2041 ctgcgccgcc tccgagagat ggcggtcggt ctgtcgaagc ttcggcaata cggaaagtat
     2101 gacataaggc aaattaaacg tgacgtgagc tattaaaagc gtggggaagc ccaaaacgct
     2161 gctcgacgac actattttgc cggcaaatac aaaaaggagc atcatcgaaa cacccgtaac
     2221 tatttccgga ttcatcatag gtacgtttgt aacggtcata acggcacttc tcagccaccg
     2281 ttttttcatt gcatttattc cgactgccgc cgccgttccg ataataatcg aaataagagc
     2341 ggaggtaacg gctaaaacaa gtgtgttacg aagagccgta agagccgtgg cgttacggaa
     2401 catttcgaca taccatttca gcgaaaagcc cgacataacg cttgtgctgt ttgtggcgtt
     2461 aaacgagaac accaccataa cgagcatcgg ggcataaaga aataacatta taagagcgat
     2521 gtatattttt ttgagagctt tcatattatc atcgccccct cttcattatc ggcaaagcga
     2581 ttcataataa gcatacatac tattatgaga agcatcagca caagcgagag cgacgctccg
     2641 agattatagt tgtacgctga ctggaactgc atttcaatgg agtctccgat aagcagaaat
     2701 tttccgccgc cgagcttttg gctgatataa aaggtactta ccgacgggac gaacaccatt
     2761 gttataccgg agataacgcc gggcaggctt agcggaaaga taaccttttt aagcacctga
     2821 agcttacccg ctccgagatc ctgtgccgct tcaacaaggc ttttatcaag ctttgacata
     2881 acggagtata tcggcagcac catatacggc aaaaagttat ataccattcc gaggattacc
     2941 gcgcccggag tgttgataag ctgtgccgtg ggcagcccca cagcctttaa aagcgaattt
     3001 ataaggcctg tatttgagag aatcgttatc cacgaatatg tgcggataag aaaattcatc
     3061 cacatgggga gcattatgag catcattatg gttcgctgag tgttcgcacg gctctttgcc
     3121 ataatatacg cgagcggata tgcaagaata aggcaaatga ccgtagccaa aaccgcatac
     3181 cacgcagatc tgccgaagat agccccgtag gatgagagcg ccttgatatt gtcaagggta
     3241 aatgcgccgc tgcggtcggt aaaagcgtag tagattacca ttataagcgg agccaagata
     3301 aaaagcactg cccatatcat atacggagcg tttatgagct ttaaggtgag agaagacttt
     3361 ttcatggctt actctccaat ctcggagact tcgtcttcat ccgtaagctc tgcattgggg
     3421 tcggaaagct cgtcaaattc ttcgctgaac gagctgtaat cgccgtaaag acccgaatat
     3481 tcgctctttt tcattatctg aatttcatcc ggctttatcg aaatacctat acggtcgccg
     3541 acttcggctt tttcggtggt ctgtatcatc cacttaaagc ctttaatgtc aacgattatt
     3601 tcatagtgaa cgcccttaaa cgtaacagag gtaacgtcgc cggagagcat accctccccg
     3661 ggcggcacca tatctatatc ctccggtcgc acaactacat ctacgggttc gttaaggccg
     3721 aagcccttat cgacgcagcg gcagcgcata ccctggaatt cgacgttgta gtcggagagc
     3781 ataacaccgt ccacaatatt gctttcgcct atgaaatccg caacaaaagc gttctgcggt
     3841 tcattataaa tatcggtggg cgagcctatc tgctgaataa caccgccgtc cataacaact
     3901 atggtatcgg acatggagag tgcttcctcc tggtcgtgcg ttacatatat aaacgttatg
     3961 ccgagccgct gctgtat
//
LOCUS       DBMDVO010000043         3960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_9882_length_3960_cov_1.338430, whole genome shotgun sequence.
ACCESSION   DBMDVO010000043 DBMDVO010000000
VERSION     DBMDVO010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9882_length_3960_cov_1.338430"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="ACZPE2_01160"
     CDS             <1..583
                     /locus_tag="ACZPE2_01160"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1318486.1"
                     /translation="VIGYCVEMVFCYLKHGYWESRQSLIYGPFGLAYGLGGVLLSYLL
                     FKDTKKAWWKVFIKSYIWGTVAEYICSLGEELVFGHVSWDYSKMPLNINGRVCALYSV
                     FWGIMGLVWVKLIYPLFMKLIHKIPLKIGKVLFWIMFILILIDIILSCEAVSRRNARD
                     KGVAPQNRFEQFMDKHYTDEFLDKVYANAVATS"
     gene            981..2483
                     /locus_tag="ACZPE2_01165"
     CDS             981..2483
                     /locus_tag="ACZPE2_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318487.1"
                     /translation="MENKMLDDPNRKYVPKKEFVTYSVSALGQGMIYAIMSSYISDFY
                     LNVLRVTPIFVLLLMFLARIWDAINDPLMGMIMDAHEPKHGKMLTYIIATPIPIALLT
                     LSLFYAPDISMTAKMIYASVTYVLWGMIYTMSDVPFWSLPNAMTPNPSERGSILSIAR
                     TTNGIGSAVPMAIFMILGFVLPSLTGLSGLALEKTKYMSIALVASIIGNLLFVMTYFK
                     VKERVKIPKPPKRKKGEPSSLKLIFKCKPLMLVVIMGILASGRYMYQAAAIHVARYSF
                     YIGKDITGLSPEETEQALQSSISLVNTVFAVATAVGMFGAMLIVPKLIKRVSYKKLIV
                     VSCILGFVSSFITSFIGYNHFWACIPFVILSCVPCGVINCVSSAMVGDCLDYMELTTG
                     RRQNGLGNACQSFVNKLGNAVSTSVIVLMYILTELDLNKIGTSYTVNPLTMSHGIRVG
                     MFSLISIVPGISLLLCMIPMFFYDLTGDKKERVTKELAEKRVKEGMVISE"
     gene            2666..3418
                     /locus_tag="ACZPE2_01170"
     CDS             2666..3418
                     /locus_tag="ACZPE2_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318488.1"
                     /translation="MHGENKAKKWEIIYWWVLRAVMVAAFIDSVFVRKDVQQSLQTAA
                     NLLLMFLWEIFMIFPEKRWMHYVPSYIQDFLAAGLFMGSFGGAYMDLYYSFPAYDLVM
                     HSVGGVLCTFVGYEILVCMQKRDKVKVDLPIVIFGAFGISFFAGTAWELFEFVFDQVA
                     PQIGDAQHWSLALAEKAAEEHGIGLPNVIAARDPMRYALIDTMEDTVCNTVGAVVGWI
                     ILKLRPYHHTGKHNVNDLFNTYEAESKEKVKS"
     gene            3531..>3960
                     /locus_tag="ACZPE2_01175"
     CDS             3531..>3960
                     /locus_tag="ACZPE2_01175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1318489.1"
                     /translation="MKKLFKMSAVILFVLIVFSACGRNESVSGKITSFPPYGERVVTA
                     IGDSIAAGYGLDSQEDNYLTLFSDNIGAVLNNDAVSGYDSGEVLKSLSDEKTAADIKN
                     ADAIVVSVGGNDFFHRKDIMIDALKNALLHGEGFFPEEVTN"
BASE COUNT         1050 a          778 c          974 g         1158 t
ORIGIN      
        1 cgttataggc tactgtgtgg agatggtatt ttgctatctg aagcacggat attgggagag
       61 ccgccaatct cttatctacg ggccattcgg ccttgcctac ggtctcggag gcgtgctttt
      121 gagttatctt ctgtttaagg atacaaaaaa ggcttggtgg aaggtattta tcaagtcata
      181 tatttggggc accgttgccg aatacatctg ttcccttggc gaagagctgg tgttcggtca
      241 cgtatcttgg gattacagca aaatgccgct taatatcaac ggcagagtct gcgctcttta
      301 ttcggtattc tgggggatta tgggccttgt atgggttaaa ctcatatatc cgctgtttat
      361 gaagctgata cataaaatac cgcttaaaat aggtaaggtg cttttctgga taatgtttat
      421 tcttattctt atagatatta ttctgtcctg tgaagccgta tctcgcagaa atgctcgtga
      481 caagggcgtg gcgccgcaaa atcgctttga gcagtttatg gataagcact atacggatga
      541 atttctcgac aaggtttacg ctaatgccgt tgcaacgtca tgatgatgcc gcgaagcaag
      601 cacggttgcc gatacccctt ttgctgcata gtatatactg tgcagcccga tattaagtct
      661 tgagcaagcg gcggcgagat gcgcactccg gagtcatcgc gcggcattgt gctccggctt
      721 ttcggagaga gcaattaaat atagcttaca caaacgccga agtgacggct gagcgttcct
      781 tttgaacgta taccgtctct tcggcaattt tcatattccg cagcggcaat atatgtaaag
      841 caatatacat ttacacgtca ttgttcgtta tatttaacaa ataaggatat cttgtttttt
      901 acgtgaattt aacattatat tgtggtactc tttatatttt tatgttaaaa ttagataaat
      961 ttatgattgg gaggaagaca atggaaaaca agatgcttga tgaccctaac aggaaatacg
     1021 ttcctaaaaa agaatttgta acatattccg tttcagcact cgggcagggc atgatatatg
     1081 cgataatgtc gtcgtatata tccgatttct acttgaatgt gcttcgagta acacctatat
     1141 tcgtattgct tttaatgttt ttggcaagaa tatgggacgc aatcaatgac cctcttatgg
     1201 gtatgataat ggacgcccac gagccgaagc acggcaaaat gctgacttat attatcgcaa
     1261 cgcctattcc gatagctttg cttacgctct cgctgtttta cgctcccgat atttcaatga
     1321 ctgcaaagat gatatatgcg tcggttacgt atgttttatg gggaatgata tacacaatgt
     1381 ccgatgttcc gttctggagc cttccgaacg cgatgactcc gaatccttcg gagcgcggca
     1441 gtatactgtc aatagcgaga acgaccaacg gcataggctc ggccgtgcca atggctatat
     1501 ttatgatact cggctttgta cttccgtctc ttacaggact ctcgggactt gcgcttgaaa
     1561 agacaaagta tatgtcgatt gctttggtgg cgtctataat aggcaattta ctttttgtaa
     1621 tgacgtattt caaggttaag gagcgcgtca aaatacccaa gccgccgaag cgtaaaaagg
     1681 gtgagccgtc ctctttaaag ctcatattta aatgcaagcc gcttatgctt gttgttataa
     1741 tgggcatatt ggcgtcgggc agatatatgt atcaggcggc ggcaatacac gttgcaagat
     1801 attcgttcta tatcggaaag gatattaccg gcctgtcgcc ggaggagact gagcaggctt
     1861 tacagtcgag tatatctctt gtaaatacgg tgtttgccgt agcgacggct gtcggtatgt
     1921 tcggggcaat gcttatagtt ccgaagctta tcaaaagagt gtcctacaaa aagcttatag
     1981 tcgtttcctg catactcggc tttgtttcga gttttatcac atcctttata ggctacaatc
     2041 atttctgggc gtgtataccg tttgttattc tttcttgcgt tccgtgcgga gttataaact
     2101 gcgtatcttc cgcaatggtc ggcgattgcc ttgactatat ggagctgacc acgggccgca
     2161 ggcagaatgg gctcggaaat gcctgccagt cctttgtaaa taagctcggg aatgcggttt
     2221 caacaagcgt catagtgctt atgtacatcc tgaccgagct tgaccttaac aaaataggaa
     2281 cctcttatac ggtaaatccg ctcaccatgt cgcacggcat aagagtgggt atgttctcgc
     2341 ttatttcgat agttccgggc atatcgcttc ttctttgtat gataccgatg ttcttctatg
     2401 acctcaccgg cgacaaaaaa gagcgcgtga caaaagagct cgccgagaag cgagtaaaag
     2461 agggtatggt aataagcgaa taatgcctac tgccgctgta agtcttaaaa atgtaaggaa
     2521 tatcaatctt taatttattt ttaataattt tactctttac aatcattccg aatgtgttat
     2581 cctataagaa aagatttgac acaattttaa aatgtgttga aaaagaatcg gaggggtaaa
     2641 tacgaaggct tatataaatc gtatattgca cggtgaaaac aaggcgaaaa aatgggaaat
     2701 aatatattgg tgggttctca gagccgttat ggttgcggcg ttcattgatt cggtttttgt
     2761 gagaaaggac gttcagcaga gcttgcaaac ggcggccaat ttgctgctta tgttcctgtg
     2821 ggaaatattt atgatttttc cggaaaaacg gtggatgcat tatgttccgt cgtatattca
     2881 ggactttttg gcggcagggc tgtttatggg gtcgttcggc ggcgcatata tggaccttta
     2941 ttacagcttc cctgcgtatg accttgtaat gcacagcgta ggcggagtcc tttgcacatt
     3001 tgtcggatac gaaatactgg tatgtatgca aaagcgcgac aaggttaagg tcgatttgcc
     3061 gattgtgatt ttcggagcgt tcggtatatc gttctttgcc ggtaccgcat gggaactttt
     3121 cgagtttgtg tttgaccagg tggctccgca gataggcgac gctcagcatt ggtcgcttgc
     3181 tcttgccgaa aaggcggcgg aagagcacgg cataggcctg ccgaatgtca tagctgcaag
     3241 agatccaatg cgctatgcac ttattgatac catggaggat actgtgtgta atacggtagg
     3301 tgctgttgtg ggctggataa ttttaaagct tcgcccgtat caccacacag gtaagcacaa
     3361 tgtaaacgac ctttttaata catatgaggc cgaaagcaaa gaaaaagtga aaagctaacc
     3421 aaaaaggcaa ataaaaaatc cgatacggcg tataccgtat cggatttttg catatcgggg
     3481 ctttgtcggc acaaaatact gaatgcaata aaaagtgtcg gggtgctcat atgaaaaagc
     3541 tgtttaaaat gtcggctgtg attttgtttg tacttattgt tttttcggcg tgcggaagaa
     3601 acgaaagcgt cagcggaaaa attacatcat ttccgccgta cggcgagcga gtcgttaccg
     3661 cgataggcga ttcgatagcc gcaggctacg ggctggattc acaggaggat aattatttga
     3721 cgctgttttc cgataatatc ggagcggtat tgaataatga cgccgtcagc ggttatgaca
     3781 gcggcgaggt gctgaaaagt ctgtcggacg aaaagactgc ggcagatata aaaaatgccg
     3841 atgcgattgt tgtatccgta ggcgggaatg atttttttca ccgcaaggat attatgatag
     3901 acgcacttaa aaacgccctg ctgcacggcg agggcttctt cccggaggaa gtgacaaata
//
LOCUS       DBMDVO010000044         3888 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10158_length_3888_cov_1.167943, whole genome shotgun sequence.
ACCESSION   DBMDVO010000044 DBMDVO010000000
VERSION     DBMDVO010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_3888_cov_1.167943"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZPE2_01180"
     CDS             <1..289
                     /locus_tag="ACZPE2_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1318490.1"
                     /translation="KKYANRVKHVHLKDVRPEVIDKVKKENLSFLEGVRLGTFTVPGD
                     GAIDFGPIFDILADSGYEGYVLVEAEQDPAKANPFEYALKARKYIAEKAGL"
     gene            308..1462
                     /locus_tag="ACZPE2_01185"
     CDS             308..1462
                     /locus_tag="ACZPE2_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1318491.1"
                     /translation="MSFQFIMPKQIFYGENALSTAAEHICALGKKALVVTDPMMVKLG
                     NTAKITDILDKEGTRYAIFDGVISEPTDRIIEAGLKVWNNEKCDFLIAVGGGSPIDAM
                     KAIGAVATSGCSVNDFLGKIITVPTPPMVAIPTTSGTGSEATQFTIITNTEKDIKMLL
                     KGAVLMPDLAIDDPAFTMTAPPSVTAATGLDALCHASEAYTSRKAQPMTDDLAISAVK
                     RIFKYLPTAYREPQNIEAREQMSLAALEAGTAFNNASVTVIHGMSRPIGALFHVPHGI
                     SNAMLMAECFDYVYDGAEKRFADMARAIGKAASDDSDSAAARKFVDACKELCDICGIP
                     SVSGYGIDLVDFRKAMPKMAEDAFASGSPSNTIKDISVEDILKIYDRLCK"
     gene            1635..2648
                     /gene="iolG"
                     /locus_tag="ACZPE2_01190"
     CDS             1635..2648
                     /gene="iolG"
                     /locus_tag="ACZPE2_01190"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIR1318492.1"
                     /translation="MLKIGIIGAGRIGKVHLESISYHVKNATVTAMADPFMNEETEKL
                     IRSYGVSKVTKDYKDILNDKDIDAVLVCSSTDTHAAISIEAINAGKHVFCEKPVDHSI
                     EKIQAVADALKEHPDIKFQVGFNRRFDHNFAAIRKAYDDGKIGEAHILKITSRDPEPP
                     NPAYIKVSGGIFLDMTIHDFDMACFLTDSDVEELYVNSAVLVDPAIGEQGDVDTAIIT
                     MKMANGALAVIDNSRKAAYGYDQRAELFGSKGMVATSNDTVSSAVISNADGVTGEKPL
                     FFFLERYMDSFSEEMRQFTEAVINDTEVPVGIHAGLQSVKIGLAARKSVEEHRPVKIS
                     EIQ"
     gene            complement(2743..>3888)
                     /locus_tag="ACZPE2_01195"
     CDS             complement(2743..>3888)
                     /locus_tag="ACZPE2_01195"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1318493.1"
                     /translation="GILNWDYTGLTKYYGTWYYVQNGILNWDYTGLTKYYGTWYYVQN
                     GILNWDYTGLTKYYGTWYYVENGVLNWDYTGLTKYYGTWYYVENGVLNWNYTGLTKYY
                     DTWYYVKEGVLDWSYTGLTKYYGTWYYVYGGILRWDTNTLVKYGDDWYVVSGGVVDFG
                     YNGAYVYGDTLNAIDGGVWNKNYNGPIYYDGYAYTLTNGTLYSYYLHPQAVNHNAPYL
                     IAVDRTNNCITVYAKDNSGKFTVPDRAFVCSVGTDGLTPVGVFNTPAKYRWKELRGNV
                     HGQYSTRIVGKVLFHSVPYSKQDNSTLLYRSYNKLGSAASHGCVRLTTADAKWIYDCC
                     PLGTTVMIYDSWGGTVLPKPSAVKISASDGRRGWDPTDPDPANPWRR"
BASE COUNT         1104 a          942 c          926 g          916 t
ORIGIN      
        1 gaaaaaatac gcaaacagag taaaacacgt gcacttaaag gatgttcgcc ccgaggtaat
       61 agataaggtt aagaaggaaa acctcagctt ccttgaaggc gtgcggctcg gaacgttcac
      121 ggttcccggc gacggcgcga tagacttcgg cccgatattt gacattcttg cggacagcgg
      181 ctatgagggt tacgttctcg tagaggcgga gcaggaccct gccaaggcaa acccgtttga
      241 atacgcgctc aaggcaagaa aatacattgc cgaaaaagcc gggctttaat ttagcggagg
      301 ttaagaaatg agttttcaat ttattatgcc gaaacaaatt ttttacggtg aaaacgcctt
      361 atcgaccgca gcagagcata tatgcgcctt gggcaaaaaa gctcttgtgg ttaccgaccc
      421 catgatggta aagctcggca acaccgcaaa gattacggat atacttgaca aagaaggcac
      481 tcggtacgca atttttgacg gcgtcataag cgagcccacc gacaggatta tcgaagcagg
      541 cttaaaggta tggaacaatg aaaaatgtga cttcctgata gccgtaggcg gcggctcgcc
      601 gatagatgct atgaaggcga taggtgcggt tgcgacaagc ggctgctccg tcaacgattt
      661 tctcggtaag ataattaccg tccccactcc gccaatggta gcaataccga caacgagcgg
      721 taccggctcg gaggctacgc agtttacaat aataacaaac acggaaaagg acataaaaat
      781 gctcttaaag ggcgcggtgc ttatgcccga ccttgcaata gacgatccgg cgtttaccat
      841 gaccgctccg ccatccgtta cggccgctac cggacttgac gcactttgcc acgcaagcga
      901 ggcgtacaca tcaagaaaag cacagccaat gacggacgat ctcgctatca gcgcggtaaa
      961 aagaattttc aaatatctcc ccacggctta tcgcgagccc caaaacattg aggcaagaga
     1021 gcaaatgagc cttgcggctc ttgaagccgg cacggcgttc aacaatgctt ccgttaccgt
     1081 aattcacggt atgtcacgac cgataggcgc gctgttccat gtgccgcacg gaataagcaa
     1141 cgccatgctt atggcggaat gcttcgacta cgtttacgac ggcgcagaaa agcgctttgc
     1201 tgatatggca cgggcaatag ggaaagccgc aagcgacgat tccgactccg ccgcagcaag
     1261 gaaattcgtt gatgcctgca aggagctttg cgacatctgc ggcataccga gcgtatcggg
     1321 ctacggaatc gaccttgtag atttccgcaa agcgatgccg aaaatggccg aggatgcctt
     1381 cgcatccggc tcaccgtcta atacgataaa ggatatatcc gttgaagata tattgaagat
     1441 ttacgacaga ctttgtaaat aatttttcgg atacaaacag tataaatctc gctgtttaaa
     1501 tcatatttca ctcaaaattt gccgacggca caattttgaa acagcagaaa tcaccggtta
     1561 tacctgtcag ggcaacacgg gacgcagggt gattttaaat aactatttgt tgcctgaaag
     1621 gttatgtgat aattatgtta aaaatcggca tcatcggcgc aggacgcatt ggcaaggtgc
     1681 atcttgagtc aatctcctac cacgtaaaaa atgcaacggt aaccgcaatg gcagatccgt
     1741 ttatgaacga agaaaccgaa aagcttataa gaagctatgg ggtttcaaag gtgacaaaag
     1801 actataagga cattttgaac gacaaggata ttgacgccgt tcttgtctgc tcctcaaccg
     1861 acacacacgc cgcaatttcg atagaagcca taaacgccgg aaagcacgtt ttctgcgaga
     1921 agccggttga ccactcaatc gaaaaaatcc aagccgtcgc agacgcttta aaggagcatc
     1981 cggatattaa gttccaggtg ggcttcaacc gccgctttga ccataatttt gccgctatac
     2041 gcaaggctta tgacgacgga aaaataggtg aagcgcacat actgaaaatt acctcgcgcg
     2101 accccgaacc gccgaatccc gcatacataa aggtatcggg aggtatcttc ctcgatatga
     2161 cgattcacga tttcgatatg gcgtgtttcc tcaccgacag cgacgttgaa gagctttatg
     2221 taaactccgc ggtgctcgtt gatccggcca taggcgagca gggcgacgtt gacacggcaa
     2281 tcataaccat gaaaatggca aacggcgctt tggcggttat agacaattcg cgcaaggcgg
     2341 cttacggcta tgaccagcgc gccgagcttt tcggctctaa gggtatggtt gcgacgagca
     2401 acgacaccgt atcctcggcg gtaatatcaa acgctgacgg cgttaccggc gaaaagccgc
     2461 tcttcttctt ccttgagaga tatatggact cattctccga agaaatgcgt caattcacag
     2521 aggcggtcat aaacgatacc gaggttcccg tgggtattca cgcaggcctt cagtctgtaa
     2581 agataggtct tgccgcaaga aaatcggtag aggaacacag acccgtaaag atttccgaaa
     2641 tacagtaata taaataccaa aataaataaa tcccgaacaa aggccgcaca tttataggcc
     2701 tactgttcgg gattttattt ttaatttaat ttaatcgttt aatcatcttc tccacggatt
     2761 agcgggatcg ggatctgtcg gatcccagcc gcggcgaccg tcagaggcac tgattttaac
     2821 cgccgagggc ttcggaagaa cagttcctcc ccagctgtca tatatcataa ccgttgttcc
     2881 gagcggacag caatcatata tccacttggc gtcagcagtt gtaagtctga cgcagccgtg
     2941 agaagcagca ctgccgagct tattatagct cctgtaaagg agcgtggaat tgtcctgctt
     3001 tgaatacggt acggagtgaa aaagcacctt accgactatt ctcgttgaat actgcccgtg
     3061 gacgttgccc ctaagctcct tccagcgata ttttgccgga gtgttgaaaa ctccgacggg
     3121 ggttaagccg tccgttccca ccgagcacac aaaggctctg tccggcacgg taaatttacc
     3181 gctgttatcc tttgcataaa ccgtgataca gttatttgtg cgatcaaccg ctataagata
     3241 aggcgcattg tgatttaccg cctgtgggtg aagataatag ctgtataacg taccgtttgt
     3301 gagagtatag gcatagccgt cataatatat ggggccgttg taatttttgt tccaaacgcc
     3361 gccgtcaatg gcattaagag tgtcaccgta aacgtatgca ccgttataac cgaaatctac
     3421 aacaccgccc gatacgacat accaatcgtc accgtattta acgagagtat tggtgtccca
     3481 gcgcaatatg ccgccgtata cgtaatacca cgtgccgtaa tacttggtaa gaccggtata
     3541 gctccagtcg agaacgcctt cctttacgta ataccatgta tcgtaatact tggtaaggcc
     3601 tgtgtaattc caattaagta cgccgttttc gacgtaatac cacgtaccgt aatacttggt
     3661 aagacctgta taatcccaat taagtacgcc gttttcgacg taataccacg taccgtaata
     3721 cttggtaagg cctgtataat cccagttaag tatgccgttt tgcacataat accacgtacc
     3781 gtaatacttg gtaaggcctg tataatccca gttaagtatg ccgttttgca cataatacca
     3841 cgtaccgtaa tacttggtaa gacctgtata atcccagtta agtatgcc
//
LOCUS       DBMDVO010000045         3870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10227_length_3870_cov_1.562218, whole genome shotgun sequence.
ACCESSION   DBMDVO010000045 DBMDVO010000000
VERSION     DBMDVO010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10227_length_3870_cov_1.562218"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="ACZPE2_01200"
     CDS             complement(<1..857)
                     /locus_tag="ACZPE2_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1318494.1"
                     /translation="MADDKKFGNNDFEDIYSSSEKNGYEDIYSDSEEEGRAVSSDKKG
                     DDDFEDFFTGRDEKSDNEDFGNEAPVPRHREINTSPEVKPYSYNYNRAANSRAKKVSD
                     EHGFTDISSGRQPREKKPHRHSAGKIIGIVILILLLLCAAFAAVTYHQAKKLLGEVNY
                     SPLQENEYIDSSKLAHSDSVRNILLIGVDAREGESSDSTRSDTMMILSIDEKNKQLKL
                     TSILRDTYVEIPGWKWNKINAAQSHGGRQLLVDTIEYNFKVDIDNYMLVNFDMFTTII
                     DELGGVDVEV"
     gene            1081..1812
                     /locus_tag="ACZPE2_01205"
     CDS             1081..1812
                     /locus_tag="ACZPE2_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR1318495.1"
                     /translation="MLGRFVKVRVTRPIGFFDGNSKTQYRLNYGTVESGLEPHSPVKG
                     AFIMGINHPVRNFDGRVIATVKIPKTKGVIIVVSPKSKRFIVNDVRDALAFMYKKGSY
                     SIDCLYERSCGAIVYREIAGERRFLLIKNKRSANWGFPKGHVEEGESNEDTARREVLE
                     ETGLHIAILPEFKSRSEYTIQGRIEKTVIIYIASTKDTRTVIQPEEIEDYIWLSYDNA
                     YKTLNYENDKAILKKAKAYLTEHSI"
     gene            1829..2374
                     /locus_tag="ACZPE2_01210"
     CDS             1829..2374
                     /locus_tag="ACZPE2_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1318496.1"
                     /translation="MLSTFSQYFMTLSATENLAEKMVHLFGDTIPSWLTVLIISLFPI
                     LENRGGMIAARLLNMNMLQAFIISYLGNLVPIPFILLFIKKIFEWLRKIKGFDKIIKH
                     FEAKAEKNRDKINRGKQWGLLLFVAIPLPGTGGWTGALMASLLEIPPKKAFPIIALGV
                     LIAGLIMTTVTYGIGWIVSGS"
     gene            2578..3726
                     /gene="fucO"
                     /locus_tag="ACZPE2_01215"
     CDS             2578..3726
                     /gene="fucO"
                     /locus_tag="ACZPE2_01215"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR1318497.1"
                     /translation="MANRFVLNETSYHGKGAIESIADEAKGRGFKKAFICSDPDLLKF
                     GVTKKVTDVLDKAGLDYEIYSDIKANPTIENCQHGVEAFKASGADYLIAIGGGSSMDT
                     SKAIGIVVANPEFSDIRSLEGVAPTKNKAVPIFAVPTTAGTAAEVTINYVITDAEKNR
                     KMVCVDVHDIPVVAFVDPDMMSSMPKGLTAATGMDALTHAIEGYITKGAWELSDMFHL
                     KAIEIISRALRGAVENTPEGREEMALGQYIAGMGFSNVGLGIVHSMAHPLGALYDTPH
                     GVANAIILPTVMEYNAPATGEKYRDIAKAMGVKGTDDMTQEEYRKAAVDAVKRLASDV
                     GIPADLKEIVKEKDIQFLSESAYADACRPGNPRDTSVEEIAELYRSLI"
BASE COUNT         1042 a          839 c          898 g         1091 t
ORIGIN      
        1 acttcaacat ctacgccgcc gagctcgtcg attatagtgg tgaacatatc aaagttcaca
       61 agcatatagt tatctatatc cactttgaaa ttgtattcta ttgtgtctac aagcagctgc
      121 ctgccgccgt ggctctgcgc cgcgtttatc ttgttccact tccagcccgg aatttcaaca
      181 tatgtgtcgc gaagaatgga ggtgagcttt aactgcttgt tcttttcgtc aatggaaaga
      241 atcatcatgg tgtcggacct tgtgctgtct gagctttccc cctcgcgcgc gtcaacgcct
      301 atcaggagaa tgttacgtac cgaatccgaa tgtgcgagct ttgaagaatc tatgtattcg
      361 ttttcctgaa gcggagagta gttgacctcg ccgagcagct tctttgcctg atggtatgta
      421 actgcggcaa aggcggcgca gagcagcagt aaaatcaata tgactatacc gattatcttg
      481 cctgcgctgt gtcggtgagg ctttttttct ctcggctgcc tgccgcttga aatatcggta
      541 aagccgtgct cgtcggaaac ctttttggct cttgaatttg cggctctgtt atagttgtag
      601 ctgtaaggtt tgacctccgg gcttgtgttt atctctctgt ggcgcggaac gggagcctcg
      661 tttccgaaat cttcgttatc cgatttttcg tctctccccg tgaagaagtc ctcgaaatcg
      721 tcatcaccct tcttgtcgga agagactgca cgtccctcct cctcgctgtc gctgtatata
      781 tcctcgtaac cgttcttttc ggaagaactg taaatatcct caaaatcgtt attgccgaat
      841 ttcttgtcat ctgccataat tgacaccctt tcacacaaca gcttataata taatgagtat
      901 attttatcgt aaattactgc ttttcacaat cccttttgat taaaatttta ttacaaaaag
      961 agtaatttta atgtcaattt aagcattgag gcgtgatttg agacttaatg tttaaatttg
     1021 tcttgaaacg agcgaaaaaa acctgtataa tgttttcaga tttttctaag gagaaccacg
     1081 atgctgggaa ggtttgtcaa agtgagggtc acaagaccca taggattctt tgacggcaac
     1141 agcaagacgc agtaccgcct taattacggt accgtcgaaa gcggacttga gccgcactct
     1201 cccgtaaagg gcgcctttat tatgggcata aatcaccccg taagaaattt tgacggcagg
     1261 gtaatagcca ccgttaaaat acctaagaca aaaggcgtaa ttattgttgt gtcgccaaag
     1321 agtaaaagat ttatagtaaa tgatgtgcgt gacgcgctcg cgtttatgta caaaaaaggc
     1381 tcctattcga tagattgtct ttatgagcgc agctgcggcg cgatagttta ccgggaaata
     1441 gcgggcgaaa gaagattttt gcttatcaaa aacaagcgca gcgcaaattg gggcttcccc
     1501 aaaggccatg tcgaagaggg cgagagcaac gaggataccg caagacgcga ggtccttgag
     1561 gaaaccgggc ttcatatagc cattcttccg gaatttaaga gccgttcgga gtatacgatt
     1621 cagggcagaa tagaaaaaac ggttataatt tatatcgcct ccacaaaaga tacaagaacc
     1681 gttattcagc cggaggaaat tgaggactac atttggttaa gctatgataa tgcttataag
     1741 acccttaatt atgaaaacga caaggctatt ctcaaaaaag caaaggctta tttgacggag
     1801 cactctatct gattataagg agaattatat gctatctaca ttttcgcagt actttatgac
     1861 gctgtccgcg acagaaaatc tcgcggaaaa aatggtgcat ctgttcggag acaccatacc
     1921 gtcatggctt acggtgctta taatttcgct gttcccgatt cttgagaacc gcggcggtat
     1981 gatagccgca agactgctca atatgaatat gcttcaggca ttcatcatct cgtatctcgg
     2041 gaatcttgtg ccgataccgt ttatcctttt gttcatcaaa aagatattcg agtggctgag
     2101 aaagataaag ggctttgaca aaataataaa gcactttgaa gcaaaagccg aaaagaatcg
     2161 agataaaata aaccgcggta agcagtgggg gcttttgctg ttcgttgcga ttccgcttcc
     2221 cggaaccggc ggctggacgg gcgcgcttat ggcgtcgctt ttggaaatac cgcctaaaaa
     2281 ggcatttccg ataatcgccc tcggcgtact tatagccggg cttataatga ccaccgtgac
     2341 atacggcata ggctggatag tttccggctc gtaagtctta tatgatttta tgattccaag
     2401 acctgttttc gctttgcggc gagggcaggt cttttaagtg tcaaaaattt ttttgattat
     2461 ttgtgagtat tgcctattga tttttcaaaa aacggttgtt aaaattttaa cgtagatggt
     2521 taaacgttta cccacgttgc cgttaaattt attttattga atacggagga ataagttatg
     2581 gctaacagat ttgttcttaa tgagacttct tatcacggca agggcgcaat agagagcatt
     2641 gccgacgagg caaagggcag aggctttaaa aaggctttta tttgctcgga ccccgatctt
     2701 cttaaattcg gagttaccaa aaaggttacg gatgttcttg ataaagccgg acttgactat
     2761 gagatatact ccgacatcaa ggctaatccc acaatagaaa actgtcagca cggcgttgag
     2821 gcatttaagg cttcgggtgc cgattatctt atcgcaatcg gcggcggctc ctcaatggat
     2881 acctctaagg cgattggcat agttgttgcg aatcccgaat tttcagatat cagaagcctt
     2941 gagggagtgg ctcccacaaa gaataaggct gttccgattt ttgccgttcc cacaaccgca
     3001 ggcacagccg ctgaggttac cataaactat gttataaccg acgccgagaa aaacagaaaa
     3061 atggtgtgcg tcgatgttca cgatatcccg gtagttgctt ttgttgaccc ggatatgatg
     3121 tcgagtatgc caaaggggct tactgccgca accggtatgg acgctcttac acacgctatt
     3181 gagggctaca tcacaaaggg tgcttgggag ctttccgata tgttccattt aaaggcaatc
     3241 gaaataattt cccgcgctct tcgaggtgct gttgaaaaca cccccgaggg cagagaggaa
     3301 atggctctcg gtcagtacat agccggaatg ggcttctcaa atgtcggcct cggcatagtt
     3361 cactctatgg ctcatccgct cggcgcgctt tacgacacac ctcacggtgt tgcaaatgca
     3421 attattcttc cgactgttat ggaatataac gctccggcta ccggtgaaaa atacagagat
     3481 atagcaaagg ctatgggcgt aaagggtacg gacgatatga ctcaggagga gtacagaaag
     3541 gctgctgttg atgcagtaaa acgccttgct tccgatgtag gtattcccgc agaccttaaa
     3601 gaaatcgtta aagaaaaaga tatacagttc ctctcggagt ctgcgtatgc ggatgcctgc
     3661 cgtcccggta acccccgcga cacaagcgtt gaagagattg cagaactcta tcgttcgctt
     3721 atctgacgca ttattactca acaataacga aattttggtt tcctcgctta agtgacgagc
     3781 cgccgtaatg gcggctcgtc actttttttt acggtacttt acacgcgact tgtgttttga
     3841 tattatatta tcgatatatt atcgatttaa
//
LOCUS       DBMDVO010000046         3865 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10255_length_3865_cov_1.324655, whole genome shotgun sequence.
ACCESSION   DBMDVO010000046 DBMDVO010000000
VERSION     DBMDVO010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10255_length_3865_cov_1.324655"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(255..1355)
                     /gene="hemW"
                     /locus_tag="ACZPE2_01220"
     CDS             complement(255..1355)
                     /gene="hemW"
                     /locus_tag="ACZPE2_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1318498.1"
                     /translation="MNNIGLYIHIPFCNSKCPYCDFYSFRADDDTKNRYVSAVLRELR
                     NNSKQFNCKADTLYIGGGTPSVLAPDYLCQIINGAKSTFLTDDAEITVECNPFSLGDE
                     FFEKVSECKVNRLSIGLQSANDGERRILGRRAGKSEVLGVVSRAKKAGINNISLDVML
                     GVPGQTKESLKSTLDFCISSGVPHLSCYILKIEENTPFFRMRDKLPFPSDDDTADMYL
                     FMCEYLEEHGIMQYEISNFAVPGFESRHNLKYWHDEEYLGIGASAHSFLGGKRTYYPR
                     DIEYFISGNERLTEGDGGDEEEFCMLALRLREGLTQERFYARFHHEIPQKMLVSAKQY
                     EKYGLCSCESGGISLTRRGFLLSNSVISEIIL"
     gene            complement(1348..3159)
                     /gene="lepA"
                     /locus_tag="ACZPE2_01225"
     CDS             complement(1348..3159)
                     /gene="lepA"
                     /locus_tag="ACZPE2_01225"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1318499.1"
                     /translation="MSGKKDYIRNFCIIAHIDHGKSTLADRLLELTDSVAKRDMEAQL
                     LDNMDIERERGITIKARAVKLDYKAADGKKYELNLIDTPGHVDFNYEVSRSLAACEGA
                     VLIVDASQGIEAQTLANTYLALDHDLEIVPVINKIDLPAAEPERVAKEVEDVIGIPCM
                     DSPRISAKTGENVPAVLEEIVNLVPPPDDNDSKPLRALVFDSVYDSYRGVIVYVRIVD
                     GVVRPGQTMRLMATGAEFTIVETGYMRAKSLEPTKQLSSGEVGYITASIKTVGDARVG
                     DTVTLAENPASEPLAGYRKVNPMVFCGIYPADGADYEALRDALAKLQLNDASLSFEPE
                     TSSALGFGFRCGFLGLLHLEIIQERLEREYDLDLVTTIPSVVYKIHLTDGNVVEIDNP
                     CNYPDPATIDFAEEPMVEAHIFSPKDYVGAIMDLCQDRRGEYKEMTYLDADRVDLKYI
                     LPLNEIIYDFFDVLKSRTRGYASFDYELCGYQRSSLVKLDVLLNGDMIDALSFIIHSE
                     KAYPRARRIAEKLKDNIPRQMFEIPIQIAVGGKVIARETVKAMRKDVLAKCYGGDITR
                     KKKLLEKQKEGKKRMRKFGTVEVPQEAFMAVLKLDDE"
     gene            complement(3231..3851)
                     /locus_tag="ACZPE2_01230"
     CDS             complement(3231..3851)
                     /locus_tag="ACZPE2_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013406.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HIR1318500.1"
                     /translation="MGYGISRAMRFGVARGVFSNEAGLGSSVMVHSSSDAKEPVTQGM
                     WGIFQVFFDTIVICTLTALVILTTGANLQSGLEGVNITIYAFESVLGKWGSYIVSIGI
                     VMFAFSTILGWSVYGSRAAEYIGGKKLTAVYKIIFLAMTFIGAVSSIQLVWDLSDTFN
                     GLMALPNLIGLLLLSGTVFKITKNYEQRVIGGKKIKPMLSFYDREK"
BASE COUNT         1022 a          978 c          815 g         1050 t
ORIGIN      
        1 aaaattttag attacgagca cacttcgaca aaaattgccg ccgttttggg taatcttgta
       61 caaattatat accggaaatt ctatgtaatc aattctcttt catagaactc ttccgtttag
      121 cctcttgatt ttatcacgtg ctgtgcgaca aaaaaacgcc attgtaacaa aaagccgttt
      181 taatttcaca aacgccagaa aagcacaaaa aaacgggctt gtaaagcaaa ttacaagccc
      241 tgaaatattt atatctataa gataatttcg gatattacgg agttggagag taaaaatccg
      301 cgccgcgtca gagaaattcc gccgctctca cagctgcaaa gcccgtattt ttcgtattgc
      361 tttgcgctta caagcatttt ttgcggtatc tcatgatgaa aacgggcgta aaaacgctct
      421 tgtgtaagcc cctcgcgaag ccttaaagcg agcatacaaa attcctcttc gtcgccgccg
      481 tcgccctcgg ttaaacgctc gttgccgctt atgaaatact caatatcacg cggataatat
      541 gtgcgttttc cgcctaaaaa cgaatgtgcc gaagcaccta tgccgaggta ctcctcatcg
      601 tgccaatatt tcagattgtg gcggctctca aagccgggaa ccgcaaaatt tgatatttca
      661 tactgcataa taccgtgttc ttcgaggtat tcgcacataa aaaggtacat atcggccgta
      721 tcgtcgtcag aggggaacgg caatttgtct ctcatacgaa aaaacggcgt attctcctct
      781 attttgagaa tgtagcagct gagatgcggc acacccgaag aaatgcagaa atcaagcgtg
      841 cttttaaggc tctcttttgt ttgacccggc acgccgagca tgacatcaag cgaaatatta
      901 tttattcccg ctttttttgc ccgtgaaacg acaccgagaa cctcgctttt gccggctcgt
      961 ctgcctaaaa tcctacgttc gccgtcattt gccgactgca agcctatgga aagcctattt
     1021 actttacact cgcttacttt ttcaaagaat tcgtcgccga gcgaaaacgg gttgcactct
     1081 accgttattt ccgcatcatc ggtcaaaaac gttgattttg cgccgtttat gatttgacaa
     1141 agataatccg gagccaaaac cgacggagtt ccgccgccaa tgtaaagcgt gtcggcttta
     1201 caattaaact gttttgaatt gttccgaagc tctcgcaaaa cagccgaaac atatctgttt
     1261 tttgtatcgt catcggcgcg aaacgagtaa aaatcgcaat acgggcactt tgaattacaa
     1321 aacggaatgt gaatatacag ccctatatta ttcatcgtct aatttaagca cagccataaa
     1381 cgcctcttgc ggaacttcta ccgtaccgaa cttgcgcata cgctttttac cctctttttg
     1441 cttttcaagc agcttctttt tacgcgttat atcgccgccg tagcatttag cgagcacgtc
     1501 cttacgcatt gcctttacgg tttcgcgcgc tattaccttg ccgcccacgg ctatctgaat
     1561 gggaatttca aacatctggc gcggaatatt gtccttaagc ttttcggcta ttctgcgcgc
     1621 tctcggatat gccttttcgg agtgaataat aaacgagagg gcgtctatca tatcgccgtt
     1681 caaaagaacg tcgagcttta caaggcttga cctttgatag ccgcaaagct catagtcaaa
     1741 cgaagcatag ccgcgcgtac gcgattttaa gacgtcaaag aagtcataga ttatttcgtt
     1801 aagcggaagt atatatttta agtccaccct gtcggcgtca agatacgtca tttctttata
     1861 ttcgccgcgt ctgtcctggc aaaggtccat tatggctccc acataatcct taggagaaaa
     1921 gatgtgcgcc tccaccatgg gttcctctgc aaaatcgatt gttgcggggt cggggtagtt
     1981 gcacggattg tctatttcga ctacgtttcc gtcggtaaga tgtattttgt aaacaaccga
     2041 aggtattgtt gttacgaggt cgaggtcata ctctctctcg aggcgttcct gtatgatttc
     2101 aaggtgcaaa agccccaaga aaccgcacct gaagccgaag ccgagggcac ttgacgtctc
     2161 gggttcaaac gagagcgagg cgtcattcag ctgtaatttg gcaagagcgt ccctaagagc
     2221 ctcataatcc gctccgtccg ccggataaat tccgcagaaa accatcggat ttaccttgcg
     2281 atatcctgca agcggctccg aagccggatt ttcggcaaga gtcacggtat cgcctacgcg
     2341 cgcatcgcct acggttttaa tcgaagcggt gatataaccg acctcacccg aggacagctg
     2401 ctttgtcggc tccaagctct tggcgcgcat atatcccgtt tccacaattg taaattcggc
     2461 acccgtagcc ataagacgca tggtctgccc cggccttacc acaccgtcaa ctattctgac
     2521 atagacaatt acaccgcgat agctgtcata tacggagtca aatacaagcg ctctgagcgg
     2581 ctttgaatcg ttatcatccg gaggcggcac aaggtttacg atttcctcga gcacagcggg
     2641 aacattctcg cccgtttttg ccgaaattcg cggcgaatcc atacacggta tgcctataac
     2701 gtcctccact tctttagcaa cgcgctccgg ctctgcggcg ggaaggtcga ttttatttat
     2761 taccggtaca atttcaaggt cgtggtcaag cgcaaggtag gtatttgcaa gagtttgagc
     2821 ctctatcccc tgtgacgcgt caactatcag cacagctccc tcgcatgccg caagggaacg
     2881 cgacacctca tagttgaagt caacatgacc gggagtgtct attaagttca gttcatattt
     2941 tttgccgtcg gcagccttgt aatcaagctt tacggcacgt gccttaatgg ttataccgcg
     3001 ttcacgctca atatccatat tgtcaagaag ctgcgcctcc atatcacgtt tcgctacgga
     3061 gtccgtaagc tccaacaaac ggtcggcaag cgtactcttg ccgtggtcga tatgggcaat
     3121 tatgcaaaaa tttctgatgt agtccttttt acctgacata aatttcctct tttatctttt
     3181 taaatcccgt atattatatc aaattcggag ttaaatgtga ataccgaaaa ttatttctca
     3241 cggtcataaa aagacagcat cggctttatc tttttgccgc ctatcactct ctgctcgtag
     3301 tttttggtta ttttaaacac cgtccccgac aaaagcaaca gacctataag gttcggcaaa
     3361 gccataagac cgttaaacgt atcggaaaga tcccaaacaa gctgaatgct cgacacggca
     3421 cctataaatg tcattgcgag aaatattatt ttatacaccg cagtcagctt ttttccgcct
     3481 atatattctg cggcgcgcga gccgtacacc gaccagccga gtatggttga aaaagcgaac
     3541 ataactatac ctatggatac tatataagag ccccatttgc cgagcacact ctcgaaggca
     3601 tatattgtta tattgactcc ctcaagtccg ctttgcagat ttgccccggt agtcagtatt
     3661 acgagtgccg taagtgtgca tataacaatg gtgtcaaaaa acacctgaaa tatgccccac
     3721 ataccctgtg ttacgggttc cttggcatca gaggaggaat ggaccattac ggacgagccg
     3781 aggcctgcct cattagaaaa tactccgcgc gccacaccga aacgcatggc tcttgaaata
     3841 ccgtatccca taacgcctcc tccga
//
LOCUS       DBMDVO010000047         3820 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10443_length_3820_cov_1.002420, whole genome shotgun sequence.
ACCESSION   DBMDVO010000047 DBMDVO010000000
VERSION     DBMDVO010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10443_length_3820_cov_1.002420"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            37..645
                     /gene="ruvA"
                     /locus_tag="ACZPE2_01235"
     CDS             37..645
                     /gene="ruvA"
                     /locus_tag="ACZPE2_01235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1318501.1"
                     /translation="MFYSITGTICYVDSVSVALDCNGVAFRCFTSLNTLKQIGEKGAK
                     ATLYTYLSVKEDALDLYGFSTEQELECFKALISVSGVGPKAGIAILSELTPEKLVICI
                     ASGDNKAITRAQGVGPKIAQRIVLELKDKLAKGLDLSVNTIDVSAVNSASSSGNQAEA
                     VSALTMLGYTQSEASVAVAKIDENLSVEEIIKQALKILSRQV"
     gene            649..1677
                     /gene="ruvB"
                     /locus_tag="ACZPE2_01240"
     CDS             649..1677
                     /gene="ruvB"
                     /locus_tag="ACZPE2_01240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1318502.1"
                     /translation="MDFDNENRIISPDLEETDKDIELSLRPHTLNEYIGQEKAKENLK
                     IYIEAAKTRGDVLDHVLLYGPPGLGKTTLAGIIANEMGVQIRVTSGPAIEKPGDLAAL
                     LTNLGDGDVLFIDEIHRLSKSVEEVLYPAMEDHALDIIIGKGPSARSIRLDLPRFTLV
                     GATTRAGQLTAPLRDRFGVILRLEMYTPEQLSLIVKRSAGILEIPIDEDGAREIASRS
                     RGTPRIANRLLKRARDFAEVMGNGVITGDTAKIALGRMEIDELGLDLIDRLLLTSMIK
                     NYNGGPVGLETIAATIGEEAVTIEDVYEPYLMQIGFLSKTPRGRVVTVKAYEHLKLPM
                     NGQQKLDI"
     gene            1701..3050
                     /gene="glmM"
                     /locus_tag="ACZPE2_01245"
     CDS             1701..3050
                     /gene="glmM"
                     /locus_tag="ACZPE2_01245"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1318503.1"
                     /translation="MGRLFGTDGARGVANSELTCELSMQIGRAAAMVLTENLSHRPKV
                     MIGMDTRASSEMLEASIAAGLCSVGADVLLLGVVPTPAVAFLVKEYGYDAGVMISASH
                     NPCEYNGIKIFQSTGYKLPDELENEIESIILDDAKVPPVMIGGDVGRITRSDTAADDY
                     IKHVLSTIDVRCENLKIAIDCANGSSSVSAKRIFESLGADCIIINAEPNGVNINADCG
                     STHMDGLRKCVIDNHCDLGFAFDGDADRMLAVDNEGNVVDGDQVIALCAKYMKSVGTL
                     KKDTAVVTVMSNMGFFRFCDDNGMYCEKTKVGDRYVLENMLENGYSIGGEQSGHVIFL
                     DYATTGDGQVSALQVLKVIKNTGKTLKELAGEMEIFPQVLINVKVSKYGRVRFTSDME
                     IKNAIKRAENELGNDGRVLVRVSGTEPLVRVMLEGKDKAKIQSLGEAIADVVRERLV"
     gene            3050..>3820
                     /locus_tag="ACZPE2_01250"
     CDS             3050..>3820
                     /locus_tag="ACZPE2_01250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1318504.1"
                     /translation="MRYSSAWKDYELIDCSSGERLERWGDKILIRPDPQVIWNTPKDM
                     HLWSRADAVYHRSNTGGGHWQVNGALPQSWKIAYKDLTFNVKTMGFKHTGIFPEQAVN
                     WDMVSKLIKAQNREVKVLNLFAYTGAATVAALKAGASVVHVDASKGMVQWAKENAASS
                     AVADKSVRWIVDDCIKFVKREIRRGNRYDIIIMDPPSYGRGPGGEVWKLENEVYGFVD
                     LCKDVLSDDPLLMLINSYTTGLSPSVIQYILGSVVAKKF"
BASE COUNT         1145 a          715 c          966 g          994 t
ORIGIN      
        1 gcttatgggt aaacttaaca gtatggggat aagataatgt tctattctat cacaggcact
       61 atatgctatg tcgattcggt aagtgttgca ttggattgca acggagtagc ttttaggtgc
      121 tttacaagtc ttaatacttt aaaacaaatc ggtgaaaagg gcgccaaggc tactctttat
      181 acatatttga gcgtaaagga agatgcgctt gatttatatg gattttcgac agagcaggag
      241 ctcgagtgct ttaaagcgtt gatttcggtt tccggtgtgg gacctaaagc cggaattgcc
      301 atactctcag agcttacacc ggaaaagctt gtaatttgta tagcttcagg tgacaataag
      361 gcaattacaa gagcgcaggg ggtaggaccc aaaattgcac agagaattgt acttgagctt
      421 aaagataagc ttgctaaagg tcttgattta tcggtgaata caattgatgt gtccgcggtg
      481 aattccgcaa gctcctccgg caatcaagct gaagcagtga gcgctcttac tatgttaggc
      541 tatacacaaa gcgaagcgtc ggttgccgtt gcgaaaatcg atgaaaattt atcggtagag
      601 gaaataataa aacaggcgtt aaaaattctc tcaaggcagg tataataaat ggattttgat
      661 aacgaaaaca gaataatatc tccggacctt gaggaaaccg ataaggatat tgaattatcg
      721 cttcgtccgc atactcttaa cgagtatatc ggtcaggaaa aagcaaaaga aaatctgaaa
      781 atatatattg aagcagcaaa aactcgaggc gacgtgctcg atcatgtatt gctttacggg
      841 cctcccgggc tcggtaaaac aacgcttgca ggtataattg ccaacgagat gggtgtacaa
      901 ataagagtaa cgtcgggtcc ggcaatagaa aaaccgggcg accttgcggc acttcttaca
      961 aacctcggtg acggagacgt tttatttatt gacgaaatac accgtctctc aaaaagcgtt
     1021 gaggaagtgc tctatcctgc tatggaagat catgcgcttg atattattat cggcaaagga
     1081 ccttcggcgc gctcaataag acttgatttg ccgagattta cattagtcgg tgccacaacg
     1141 cgtgcagggc agcttaccgc tccgttgcgc gacagattcg gagtaatatt gagacttgaa
     1201 atgtacacgc ccgaacagtt gtctcttata gtaaagagaa gtgcgggaat acttgaaata
     1261 cccatagacg aggacggagc aagagaaata gcgtcaagat caagaggcac tccgcgtatt
     1321 gccaaccgtc ttttaaaacg cgccagagac tttgcggaag ttatgggtaa cggagttata
     1381 acaggcgata ctgctaaaat agcactcggc agaatggaaa tagacgaatt gggtcttgac
     1441 cttatcgacc gacttctttt gacttcaatg ataaaaaact ataacggcgg acctgtaggt
     1501 cttgaaacaa tcgcagcaac cataggcgaa gaagcggtaa ccatcgaaga tgtatacgag
     1561 ccttacctta tgcagatagg atttctttca aagaccccgc gcggcagggt tgttacggta
     1621 aaagcttatg agcatttaaa gctgccgatg aacggtcagc aaaaacttga tatttaatta
     1681 tacggatagg agaatgtaaa atgggcagat tattcggaac tgacggcgca agaggcgttg
     1741 cgaacagtga acttacatgt gaattatcaa tgcagatagg aagagccgca gcaatggtgc
     1801 ttacggaaaa tctttctcac aggcctaaag ttatgatagg tatggataca agggcttctt
     1861 cggagatgct cgaggcttca atagcggcag gtctttgctc tgtcggtgcg gacgtacttc
     1921 ttttaggtgt tgtgccgact ccggctgttg cttttttggt taaggaatac gggtacgatg
     1981 cgggtgttat gatttccgca tcgcacaacc catgcgaata taacggtata aaaattttcc
     2041 agtccacggg ttataaattg ccggatgaac ttgaaaacga aatagagagt attatccttg
     2101 atgacgcaaa ggttccgcct gttatgattg gcggagatgt cggaagaata acacgctccg
     2161 atacggcggc agatgactat attaaacatg tactgtctac tattgacgta agatgtgaaa
     2221 accttaaaat agccatagac tgcgcaaatg gttcttcaag tgtttccgca aaacgtatat
     2281 ttgaatccct cggagcggat tgcataatta taaacgccga gcccaacggt gtaaacataa
     2341 atgccgattg cggttcgacg catatggacg gattgagaaa atgtgtaatc gataatcact
     2401 gtgatctcgg ttttgcattt gacggcgacg ctgacagaat gttggctgtt gacaacgaag
     2461 gtaatgttgt tgacggcgat caggttattg cgctctgcgc caaatacatg aagtccgtcg
     2521 gtactctcaa aaaagatact gcggttgtga ccgtgatgag taatatgggc tttttcagat
     2581 tttgcgatga taacggtatg tattgtgaaa agactaaagt cggcgacaga tatgtgcttg
     2641 aaaatatgct cgaaaacgga tattcaatag gcggcgaaca gtcgggccat gttattttcc
     2701 ttgattacgc tacaactgga gacggtcagg tttcggcact ccaggtttta aaagtaatta
     2761 aaaacaccgg caaaactctc aaggagcttg caggagaaat ggaaatattc ccgcaggttc
     2821 ttataaatgt taaggtgtca aaatacggca gagtccgttt cacaagcgat atggaaataa
     2881 agaatgctat taagcgcgcg gaaaatgagc ttggtaatga cgggcgcgtg cttgttagag
     2941 tttccggtac ggaacctctt gtgcgcgtaa tgcttgaggg caaagataag gctaaaatac
     3001 agtcgcttgg cgaagcaata gccgatgttg taagagaaag gttagtttaa tgcgttattc
     3061 atccgcttgg aaagattacg agcttataga ttgctcctcc ggcgagagac tcgaaagatg
     3121 gggcgataaa attctgataa gacccgatcc gcaggttatt tggaatacgc ctaaggatat
     3181 gcacctttgg agcagggcag acgctgtgta tcacaggtca aacactggcg gcggtcattg
     3241 gcaggttaac ggagcacttc cgcaaagttg gaaaattgca tacaaagacc ttacgtttaa
     3301 tgtgaaaacc atgggcttta agcatacggg tatttttcct gagcaagccg tcaattggga
     3361 tatggtaagt aagcttatta aagcccaaaa cagagaagta aaagtactta atctttttgc
     3421 atatacaggt gctgccacag tggctgcatt aaaggcaggt gcttcggtgg tgcacgttga
     3481 tgcttccaag ggaatggtgc agtgggcaaa agaaaacgct gcctcaagtg ctgttgcgga
     3541 taaatccgtg cgctggatag ttgacgattg tattaaattc gtaaagagag aaatacgtcg
     3601 aggtaatcga tatgacatta tcataatgga tcccccgtca tacggcagag gccccggcgg
     3661 agaagtctgg aaacttgaga atgaagtata cggatttgtg gatttgtgta aagacgtatt
     3721 gtcggacgat cctcttttga tgttgataaa ttcatacact acggggcttt caccgtctgt
     3781 tatacagtat attttaggct ccgttgtagc gaaaaaattc
//
LOCUS       DBMDVO010000048         3783 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10612_length_3783_cov_1.579033, whole genome shotgun sequence.
ACCESSION   DBMDVO010000048 DBMDVO010000000
VERSION     DBMDVO010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10612_length_3783_cov_1.579033"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(508..1053)
                     /locus_tag="ACZPE2_01255"
     CDS             complement(508..1053)
                     /locus_tag="ACZPE2_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318505.1"
                     /translation="MKNKLFILLLAFVLLLSACSNNSPPDDNTKEVLPELESAGSIES
                     RGEKFIENFPQDYPSFELLEYVFGSAENAPIQLVAIARNKETGSSATLFVLDENGVGQ
                     VVLASGYSATYCKEDGLQLDKNVISISLNLEISSTNSEIHDFNITVTQEENQGKVNTV
                     YSSQENIRSKVANPAEPVNGK"
     gene            complement(1050..1697)
                     /locus_tag="ACZPE2_01260"
     CDS             complement(1050..1697)
                     /locus_tag="ACZPE2_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfkD family protein"
                     /protein_id="HIR1318506.1"
                     /translation="MRKIKTILGIVFSLAIVTTMNTTVFAANDSGIPERRGVYIRECV
                     GYEPNYTNIDYTHKLLGSVSGDNTQGSSPMQITYQYEQSGTTTASIAGYANGTTEANV
                     VFAKMQAEVGVEVTGSRSWTKGTSAGVSYSIAPGKFEVLNVYIPAVRTAGRLKYKVYM
                     DGYPENVFYEYKTLTESYAPQKNSVHYKVTTSSYSAIKVPKGMTVYTPTGVYKAQ"
     gene            complement(1928..2614)
                     /locus_tag="ACZPE2_01265"
     CDS             complement(1928..2614)
                     /locus_tag="ACZPE2_01265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1318507.1"
                     /translation="MARLNVNPTRMELTNLKRKLTTARRGHKLLKDKQDELMRRFLDL
                     VRENKELRRKVEEGIEESNRHMALARSVMSDSSLDVALMMPSQKMSLDVSEKNVMSVM
                     LPVFDTKLKTADENDIYSYGFAFTSSDLDAAVKSLSDIMPYMLRLAEVEKSCQLMSAE
                     IEKTRRRVNALEHVMIPQYEETIKYISMKLDENERSSTIRLMKVKNMMLEEARRKAIE
                     HDREAALEMS"
     gene            complement(2651..>3783)
                     /locus_tag="ACZPE2_01270"
     CDS             complement(2651..>3783)
                     /locus_tag="ACZPE2_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1318508.1"
                     /translation="SEDMLGRVFDGLGRPIDGGPEIIPEKRMNVNGMPMNPAARAYPQ
                     EFIQTGVSAIDGLNTLVRGQKLPIFSASGLPHAQLAAQIARQAKVRSADEKFAVVFAA
                     MGITFEESDFFIQSFKETGAIDRTVMFSNLANDPAIERIATPKMALTAAEYLAFDKGM
                     HVLVILTDITNYADALREVSAARKEVPSRRGYPGYMYTDLASIYERAGRQRGKNGSIT
                     MIPILTMPEDDKTHPIPDLTGYITEGQIILSRDLYRRGITPPIDVLPSLSRLKDKGIG
                     EGKTRKDHSNTMNQLFSAYARGKDAKELMVVLGEAALTDIDKLYAKFADEFEKEYVSQ
                     GYNTNRSIEETLDIGWKLLRILPRSELKRISDELLDEYYDKK"
BASE COUNT          944 a          868 c          788 g         1183 t
ORIGIN      
        1 gcagggtttg gggaaggcac tccccaacaa gattcccgca ggggcaaaat gagcgaaaag
       61 cgaattttgg cacctcggta gaatcttgct ctaagaaact gccggactgc cgctcacttc
      121 ctactgccac tttttcggga aatcgcaacc agttttttca taaaaggctg cgggctggcg
      181 ttctccctct ctttctggca aagacttttc cggcggcttg gtctttccca acaataaggc
      241 gtttcagaag ctgtgttttg cccgctgctt gatagaaaac gggaatttcc ccctgctcac
      301 ttatcagtac cgatactttc tcgatttgcc aaaaatgggc gcaaaaaaag cagcaaatct
      361 ttttcagact tactgcttca tgtgccgcta tggggcagcg gttattcagt tgtaaaataa
      421 aagagggcgg tagcactttg ggctatcgtc ctcttggcta cacatttgca aagacaggcg
      481 gggctgtcaa cggtgggcga agcccattta cttgccgttg actggctcgg ctggatttgc
      541 tactttcgat ctgatgtttt cttgtgaaga atacactgta ttgactttac cttgattttc
      601 ctcctgtgtt actgtaatat taaagtcgtg aatttcagaa tttgtactag agatttccaa
      661 attcaaagaa atagaaatta cgtttttatc aagttgtaat ccatcttctt tgcaatatgt
      721 cgctgaataa ccacttgcaa gaactacctg acctacccca ttttcatcaa gcacaaataa
      781 tgtagctgat gaaccagttt ctttatttcg ggctatcgca accagttgta ttggggcatt
      841 ttcagcggaa ccaaaaacat attctaataa ttcaaatgaa ggatagtctt gtggaaaatt
      901 ttcaataaat ttctcgcccc ggctttcaat agaaccggca ctttcaagct ctggcagtac
      961 ttcttttgtg ttgtcatcag gtggagaatt attagaacaa gcactcaaca aaagtacaaa
     1021 agctaatagt aatataaaca atttgttttt cattgtgctt tatatactcc tgttggcgtg
     1081 tataccgtca ttccctttgg aacttttatg gctgagtagc tagaagttgt tactttataa
     1141 tggacagaat ttttttgtgg tgcataagat tctgttaatg ttttatattc ataaaaaaca
     1201 ttttcaggat agccgtccat ataaaccttg tattttaaac gaccagctgt tctaacagca
     1261 ggaatataaa catttaacac ttcaaatttc cccggtgcaa tactatatga gacacccgct
     1321 gatgttcctt ttgtccaact acgtgaaccg gtaacttcaa ctcctacttc cgcctgcatt
     1381 tttgcaaaaa caacatttgc ttctgttgtg ccattggcat aacccgcaat agatgcggtg
     1441 gttgttccgc tttgttcata ttgatatgta atttgcatag gacttgagcc ttgagtatta
     1501 tcgcctgaaa cagaacctaa taatttatga gtataatcaa tattcgtata attaggctca
     1561 taacctacac attctcgtat atatactcct ctacgttctg gtattcctga atcgttagct
     1621 gcaaaaacag tagtgttcat tgttgttaca atagctaaag aaaaaactat ccctaatatt
     1681 gttttgattt ttctcattat ggacttcctc ctttgcttaa aaagttatat tttgttgtgt
     1741 gtttcatcaa accattggca tcatctccct ttgttttata gtaccatatt ttagctatta
     1801 tatcaattat caaattcaat tttgcttttt tttggttaat ttttctgaat agtgaaggct
     1861 gtctatcaaa ttcttgtgtg ttactttacc gcacatgagc atttatccga ggcttcaagt
     1921 gaagcaatca gctcatttcg agagccgcct cgcggtcatg ctcgatcgcc tttcggcgcg
     1981 cctcttcaag catcatattt ttaaccttca taaggcgtat tgtagagctt cgctcatttt
     2041 cgtcgagctt catagaaatg tattttatgg tttcctcata ctggggtatc ataacgtgtt
     2101 caagcgcatt tactcggcgt cttgtctttt ctatttctgc ggacataagc tggcacgact
     2161 tttcgacctc ggcaagacgc agcatatacg gcattatatc cgacagcgat tttaccgccg
     2221 catcaaggtc ggacgatgta aacgcaaagc cgtatgaata tatatcgttt tcatcggcgg
     2281 ttttaagctt agtatcaaaa acaggaagca ttacgctcat cacatttttt tcgctgacgt
     2341 caaggctcat cttctgagac ggcatcataa gagcaacatc aagagatgaa tcgctcataa
     2401 ccgagcgcgc caaagccata tgcctgtttg actcctctat gccctcctcc accttgcggc
     2461 gcaattcctt gttttctctt acaaggtcga ggaaacggcg catcagctca tcctgcttgt
     2521 cttttaaaag cttatgtccg cgccttgcgg tcgtaagctt tctcttcaga ttagtgagct
     2581 ccattcttgt cggattcaca ttaagtcttg ccaaaaacgg ttcctccttt cgagaagtta
     2641 atgcaccggg ttattttttg tcgtaatact cgtcaagcag ctcgtcgctg atacgtttaa
     2701 gctcgcttct cggaagaatt ctgagcagct tccagcctat atcgagcgtt tcttctatgg
     2761 agcggtttgt attgtagccc tgagacacat attccttttc aaactcatcg gcaaattttg
     2821 cgtagagctt atctatatcg gtgagtgcag cctcgccgag aaccaccata agctcctttg
     2881 cgtctttacc tcttgcatat gcggagaaga gctggttcat ggtgtttgag tggtcttttc
     2941 tcgttttgcc ctcgcctatg cccttatcct taagccttga aagcgacggt aaaacgtcaa
     3001 tgggcggcgt aattccgcgg cggtaaaggt cacggctcaa aataatctga ccctctgtga
     3061 tatatcccgt aaggtcggga atcgggtggg tcttgtcgtc ctcgggcatg gtaagaatgg
     3121 gtatcatggt gatacttccg tttttaccgc gctgacgacc ggctctctcg taaatggacg
     3181 caaggtcggt gtacatatat ccgggataac cgcgtcggct cggaacctct tttcttgctg
     3241 ccgaaacctc gcggagcgcg tccgcatagt tcgttatatc ggttaaaatt acaagcacgt
     3301 gcataccctt gtcaaatgcg aggtattccg cggctgtaag tgccattttg ggcgtagcta
     3361 tacgctcgat tgcagggtcg ttcgcaagat tcgagaacat aaccgttctg tctatggcac
     3421 ccgtttcctt aaacgactga ataaagaagt cagactcctc aaaggtaata cccatagcgg
     3481 caaagacaac ggcgaatttt tcatcggccg agcgtacctt agcctgcctc gctatctgag
     3541 cggcaagctg tgcgtgcgga agacccgaag ccgaaaagat gggaagcttt tgacctctta
     3601 caagggtgtt aagtccgtct atcgcggaaa cgcccgtctg tataaattcc tgcggataag
     3661 ctctcgccgc cggattcata ggcataccgt ttacattcat tcttttttcg gggattattt
     3721 cggggccgcc gtctatgggc ctgccgagtc cgtcaaatac tctgccgagc atatcctcgg
     3781 aaa
//
LOCUS       DBMDVO010000049         3754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10734_length_3754_cov_1.260881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000049 DBMDVO010000000
VERSION     DBMDVO010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10734_length_3754_cov_1.260881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="ACZPE2_01275"
     CDS             complement(<1..436)
                     /locus_tag="ACZPE2_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318509.1"
                     /translation="MRRTIASVFVFILVVSLLLTSCGKRRTFEPSGVTGEKAETAKSQ
                     RGEIKLESDKFTFYGVQMGMTTDQVQISVGKAAQVYARNDKMFLTEDVKVNEFKNGLD
                     RVVYYIFNTNNQLVEVQYVVSSSDGVSYQNAVTLFRGIYGPDG"
     gene            complement(436..1209)
                     /locus_tag="ACZPE2_01280"
     CDS             complement(436..1209)
                     /locus_tag="ACZPE2_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1318510.1"
                     /translation="MAKTVSVINQKGGVGKTTTAVNLSAAVGALGKRVLLADIDPQGN
                     STSGYGISKRNIENSTYDMLINGVKASEVVMKTKFRNVDLLPSDMNLAGAEVEMIGMD
                     SRESLLKKALTDVWEDYDYIFLDCPPSLGIITINALCASDTFLVPIQCEYYALEGLAQ
                     LMSTVRQIKRLYNPSIELEGVLLTMYDGRLNLTSQVVAEVKKFFPQKVYKSTIPRNVR
                     LSEAPSFGEPVMYYDRNSKGAAAYADFAEEFIKKQAREN"
     gene            complement(1564..2406)
                     /locus_tag="ACZPE2_01285"
     CDS             complement(1564..2406)
                     /locus_tag="ACZPE2_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1318511.1"
                     /translation="MKSKDKIKTAAQVLLLPQDSILPNPDQPRKRFDYDELEGLAQSI
                     RQNGILQPVTVRRRTDGKNVKFELVAGERRLRAARLVGLTKIPAIETDADEKSSAVLS
                     LIENLQRQDLNFFEEAEAVQRLVDKYSMSREEAADKLGRAQSTLSNKLRLLRLPEEMR
                     YTIEKAGLTERHARALLTLENDVQRERALEIITDRHLNVQESERLIEQMLNRRKKKNP
                     IKGIRDIRVFINTLNHAVEAIRRAGIDADAAKSETEEYVEYTVRIPKIEQIRISLPGD
                     GEAV"
     gene            complement(2517..3230)
                     /gene="rsmG"
                     /locus_tag="ACZPE2_01290"
     CDS             complement(2517..3230)
                     /gene="rsmG"
                     /locus_tag="ACZPE2_01290"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1318512.1"
                     /translation="MLNRDLLYKTAENMGVKIDDDAFKRFDIYAELLFETNKKFNLTA
                     ITEPDDMTVKHFADCLSVFKYVDFKEGARLIDVGTGAGFPGLVLLLARPDLDVTFLDG
                     TGKKLAFIESVLSNVGLNGNILHSRAEEAGKDPLYREKFDFATARAVASLPVLCEYCI
                     PFIKEGGSFISMKSAFSDDEIFSASASLRILGGKIESDNVFDLVENTRRRILIIKKIS
                     QTPSKYPRASAKISKNPLG"
     gene            complement(3230..>3754)
                     /locus_tag="ACZPE2_01295"
     CDS             complement(3230..>3754)
                     /locus_tag="ACZPE2_01295"
                     /inference="COORDINATES: protein motif:HMM:NF045129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1318513.1"
                     /translation="PLGYEIGLVSEEKYQRLLKKQEMIRAERKRIEDTSIPLTDELQQ
                     LLVSRGTSPLPRGVKEVELLKRPQITYDDLTPFDVNRPDIPREIFEQVEIELKYEGYI
                     VRQTAQINEMRRLEVKAIPPETDYNNIDGLRLEAREKLSKIRPMNVGQASRISGVSPA
                     DISVLLIYLAKEGK"
BASE COUNT          878 a          943 c          862 g         1071 t
ORIGIN      
        1 ttccgtcggg accgtagatg ccgcggaaga gcgtgacggc gttctgatat gaaactccgt
       61 cggacgagga taccacatac tgcacctcga caagctgatt gtttgtattg aagatgtaat
      121 aaacaactct gtcgagtccg tttttaaatt cgtttacctt tacgtcctcg gtcaaaaaca
      181 tcttatcgtt tcttgcgtaa acctgtgccg ccttgcctac ggaaatctga acctggtcgg
      241 ttgtcattcc catctgtacg ccgtaaaagg tgaatttatc ggactcaagc ttgatttcgc
      301 ctctttgaga cttggcagtc tccgcctttt cgcccgtcac gcccgacggc tcaaaggtgc
      361 gccttttccc gcacgaagtc agcaaaagtg atacgacaag tataaaaaca aatacggatg
      421 cgatagttct cctcattagt tttccctcgc ttgcttttta atgaattcct ctgcaaaatc
      481 cgcatacgcc gccgctccct ttgaattgcg gtcataatac attaccggtt cgccgaagct
      541 cggagcctcg gaaagcctga cgttacgcgg tatggttgat ttatacacct tttgcggaaa
      601 gaacttcttt acctccgcaa ctacctgcga cgtcaaattc agtctgccgt cgtacatcgt
      661 cagaagcacg ccctcaagct ctatcgacgg attgtaaagt ctcttaatct gacggacggt
      721 ggacataagc tgcgcgagcc cctcaagtgc gtagtattcg cattgtatcg gcaccaaaaa
      781 ggtgtcgctc gcacacagcg cgtttattgt tataatcccg agtgacggcg gacagtcaag
      841 gaaaatgtag tcgtaatcct cccagacatc ggtgagagct tttttgagaa gactctcgcg
      901 cgagtccata cctatcatct cgacctcggc accggcgagg ttcatatccg acggcagcaa
      961 atcgacatta cggaatttcg ttttcataac gacttcagaa gccttcacgc cgtttatgag
     1021 catatcatat gttgaatttt caatgtttct cttgctgata ccgtagccgc ttgtggaatt
     1081 gccctgcgga tctatatcgg cgagcagaac gcgcttgccg agagcgccga ctgcggcgga
     1141 gagattgaca gccgtggtcg ttttgcctac gccgcccttt tggtttatca cggaaacggt
     1201 ctttgccata ccttttttat cactcctgtt tcagaggctt ataccccgtt tgaatacact
     1261 atttaaagta taacacaaac atcgcccaat tcaaagaggt attttattat tgtgtcagat
     1321 tttcggtgat attttccgaa agtcatcgaa ggtttcacgt ggaacattgc attttcgttg
     1381 tttttgccat tttgtttcac gtgaaacaat atgcgttttc cgcacagtaa aaaccgcaaa
     1441 gcaaaacggc gaccgaaaag gccgccgttc tgcttgtgga tgtggggtgt gaaagagaag
     1501 gatatgggta tttaaaatgc ggaacggcag gggtagtacc gttcggcgca ttttacaaga
     1561 ttgtcagacg gcttcgccgt ctccgggcag agaaattctt atctgctcga ttttcggaat
     1621 acgcaccgtg tattcaacgt actcctccgt ctcgctcttg gccgcgtccg cgtcgatacc
     1681 tgccctgcgt atcgcctcta ccgcgtgatt gagagtattt ataaaaactc ttatgtcgcg
     1741 tatgccttta attggatttt tctttttcct gcgattgagc atttgctcga taaggcgctc
     1801 gctctcctga acgtttaagt gtcggtcggt gataatttcg agcgccctct cgcgctgaac
     1861 gtcgttttcg agagtaagca atgccctcgc gtgacgttcg gtaagcccgg ctttttcgat
     1921 tgtgtaccgc atttcctcgg ggagccgcaa aagtctcagc ttatttgaaa gcgtggactg
     1981 cgccctgccg agcttatcgg ccgcctcctc gcggctcatt gaatacttgt caacaagcct
     2041 ttggaccgct tcggcctcct caaagaagtt gaggtcctgc cgctgcagat tttcaatcag
     2101 agataaaacg gcagagcttt tttcgtctgc gtccgtctcg attgccggta tttttgtaag
     2161 tccgacgagc cttgccgcac ggagcctgcg ctcaccggca accagttcaa acttcacgtt
     2221 tttaccgtcg gttcttctgc gaaccgttac gggttgaaga attccgtttt gccgtatgct
     2281 ttgcgcaaga ccctcaagct cgtcgtaatc aaaccgtttc ctcggttgat cggggttcgg
     2341 caaaatactg tcctgcggca gcagtagcac ctgtgccgca gttttgattt tgtcttttga
     2401 cttcaaaaaa atcacctccg cagtgcttgt ccttgcttaa agaatagcac cgtgaaggtg
     2461 tacttgtaaa gaaaattcgt tcgtctaaaa tcgcgtatcg ctgcggttaa aacccattat
     2521 cccaaaggat ttttagaaat ttttgccgaa gcgcgaggat attttgacgg agtttgcgat
     2581 atctttttta taatcaatat tcttctcctt gtgttttcaa ccaaatcaaa cacattgtcc
     2641 gactctattt tgccgccgag aatacgaaga gaagccgacg cagagaaaat ttcgtcatcg
     2701 gaaaaagccg atttcataga gatgaaagag cctccctctt taataaaagg tatgcaatat
     2761 tcgcaaagga cgggcagaga cgccacagcc ctcgccgttg caaagtcgaa tttttcgcgg
     2821 tacagagggt cttttcccgc ttcctcggcg cggctgtgaa gaatattgcc gtttaagccg
     2881 acattcgaga ggacgctttc tataaaggca agctttttgc ccgttccgtc aaggaaggtt
     2941 acgtcaaggt cgggtcttgc aagaagcagc acaagtcccg gaaagcctgc ccccgtgccg
     3001 acgtcaatca gccttgcgcc ctctttgaaa tcgacatatt taaatacgga aaggcagtcg
     3061 gcaaaatgct tcaccgtcat atcgtccggc tcggttatcg ccgtcagatt aaattttttg
     3121 ttggtttcaa aaagcagttc ggcgtaaata tcaaaacgtt taaaggcgtc gtcgtcgatt
     3181 ttaacaccca tattttccgc tgttttgtaa agcaaatctc tgtttaacat tatttgccct
     3241 ccttggcaag gtaaatgagc aaaaccgata tgtcggcagg tgagacgccg cttattcttg
     3301 aggcttgacc cacattcata ggccgtattt tagagagctt ttctctcgcc tcaagccgca
     3361 gtccgtctat attattataa tcggtttcgg gcggaatagc tttcacctcg agtcggcgca
     3421 tttcgtttat ctgcgccgtc tgccgcacaa tatagccctc atatttaagc tcaatttcaa
     3481 cctgttcgaa tatctcacgc gggatatcgg gtcgatttac gtcgaacggg gtcaaatcgt
     3541 catatgttat ctgcggacgc tttaaaagct caacctcttt aacgccgcgc ggcaagggag
     3601 atgttccacg tgaaacaagc agctgctgca gctcgtccgt cagaggaatc gaagtatctt
     3661 caatcctttt gcgttccgct cttatcatct cctgcttttt aagaagccgt tggtattttt
     3721 cttcggagac aagtccgatt tcgtagccga gagg
//
LOCUS       DBMDVO010000050         3694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_10974_length_3694_cov_1.111328, whole genome shotgun sequence.
ACCESSION   DBMDVO010000050 DBMDVO010000000
VERSION     DBMDVO010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10974_length_3694_cov_1.111328"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="ACZPE2_01300"
     CDS             complement(<1..1055)
                     /locus_tag="ACZPE2_01300"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1318514.1"
                     /translation="MYIAVMGYGTVGSGVVEVLHKNHDSIVSKSTQKDLELKYILDLR
                     DFPGDPNEDKFVKDFNIILNDDSVKIVVETMGGLHPAFEFVSSLLKAGKSVVTSNKEL
                     VAQKGYELLTLADENNVNFLFEASVGGGIPIIRPISQCLAANEIDEIAGILNGTTNFI
                     LTMMIEQNMSFEEALKLAQDNGYAEKDPTADVEGIDACRKICILASLCFGKHVYPDSV
                     HTEGISKITLEDVAYVNDFGGVIKLLGKARRENGGKISAMVCPCIVKNHSQLASVSDV
                     FNAILVRGDAIGDVVFYGRGAGKLPTASAVVADVIDCAKHEKRKKLFGWGAPEENYVI
                     DYRTLETALYIRAKVTG"
     gene            1269..2543
                     /gene="ytvI"
                     /locus_tag="ACZPE2_01305"
     CDS             1269..2543
                     /gene="ytvI"
                     /locus_tag="ACZPE2_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HIR1318515.1"
                     /translation="MDNIAKRRNFIINVVYIIIIAGLFYLFMKYAFGVLSPFIIAMFI
                     AMLLQRPVNFVIEKTKIPRGIVSTVAVLLVVFVFGTIIGLILSKIFSELKSFFNYIML
                     QLDNAPLFASQLKDWVNTHFTLLPDSIRQNIISTVANLIDKIQGVDTGSSSSAAAAVA
                     SSETAAKGFDFSSFSSPMSALWGTAKQIPSILVGTIVGIVCCCFMTSDYHTLRSLIMT
                     QAGKRADAFIRTKQIIFSTLGKMIRAYGLIMCITFLEMVLGLSILKLFGIYENGYIFA
                     IALITAVIDIVPVLGTGTVLIPWAIWMFFSGDVAMGIGLLVIYGVITVVRQIVEPKLV
                     ASQLGLPAFVTLMAMYIGSQLFGFVGLFLLPMTVMLLKVLNDDGVIHIFKVPPEEAAE
                     FKAAKDKDEKTDAERKAKKREGIKKIKTGKKK"
     gene            2557..3282
                     /locus_tag="ACZPE2_01310"
     CDS             2557..3282
                     /locus_tag="ACZPE2_01310"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR1318516.1"
                     /translation="MKMIDLHCHILYDIDDGAKTKEDSAALLHTAAQNEIKAIIATPH
                     FNDYSAVDEFVAKRDERVKFLREIIGGKGLDIGLGAGAEVFLQNDVFSDCDLSPLCIN
                     GSRYLLCEYTLRPFDPKYAVIYAERVLSMGLVPIIAHPERYICFHTEKWVAKELCDMG
                     ALLQVNASSLAGRGGDEIKDYATELVLSRLADFVATDAHSAVSRPNDIIKCAERFDER
                     ITSEDIKRLVYKNPLKVLKDEIL"
     gene            3318..>3694
                     /locus_tag="ACZPE2_01315"
     CDS             3318..>3694
                     /locus_tag="ACZPE2_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318517.1"
                     /translation="MEKIKRRGENYTIPQIDLSVEPKNTKEEFKYLYENVTVEKKGGY
                     LGHPDSVLLKDGSILVFFPEGHGKGKTLSKISRDGGRSYTEEIKNPPKSWENSRETPT
                     VYRLEFSESDTPDKLIMISGNPI"
BASE COUNT          978 a          806 c          915 g          995 t
ORIGIN      
        1 tttccggtga cctttgcgcg tatgtaaaga gccgtttcga gggttctgta atcaattacg
       61 taattttcct cgggagcgcc ccagccgaac agctttttgc gcttttcatg ctttgcgcag
      121 tctattacgt cggcaacaac ggcgctggct gtgggcaatt tacctgcgcc tctgccgtaa
      181 aatacaacgt cgcctatcgc gtcgccgcga acgagaatgg cgttgaatac atcgcttacc
      241 gaggcgagct ggctgtgatt tttaactatg caagggcaaa ccatggcgga aattttgccg
      301 ccgttttcgc gtcttgcctt gccgaggagc ttaataacgc cgccgaaatc gttgacatag
      361 gcgacatcct ccaaggttat ttttgagatg ccctcggtgt gaacactgtc ggggtaaacg
      421 tgcttgccga agcagagaga tgcgagaatg caaattttgc ggcatgcgtc tatgccctca
      481 acgtcggccg tcgggtcctt ttccgcgtag ccgttatcct gagcgagctt cagagcttct
      541 tcaaagctca tgttctgctc tatcatcata gtgagtataa agtttgtagt gccgtttaag
      601 atgccggcta tttcgtctat ctcgtttgcc gcgaggcact gcgaaatggg gcgaataatc
      661 ggaataccgc cgcctaccga cgcctcaaac aggaagttta cgttgttttc gtcggcgagg
      721 gtcaaaagct cgtagccctt ttgcgcgaca agctccttgt ttgacgttac aacgctcttg
      781 cccgccttta aaagcgaaga gacaaattca aatgccggat gaagtccgcc catagtctcg
      841 acaactattt tgacggagtc gtcgttcaaa ataatattga agtccttaac aaatttatct
      901 tcgttcgggt ctccgggaaa atcacggagg tcgaggatat atttgagctc aaggtctttc
      961 tgggtgcttt tgcttacgat actgtcgtgg tttttgtgaa gcacttccac aacgcccgaa
     1021 ccgacggttc cgtagcccat aacggctata tacatttttt ctcacatcct aaataatatg
     1081 aatatacatt tagcataata ccataaattc ccgacgttat aaaggaaaaa gctcaaaaat
     1141 ttttgcgttt gtttaaattt ctataaaagt ttgagattat tgattgtaag tttttgaaaa
     1201 atagtataat atgtatatat gttatcggcc ggcaaagtgt cggcttttat gttaaaggat
     1261 gtgaagctgt ggacaatatt gcaaaacgtc gtaattttat aatcaacgta gtttatataa
     1321 taataatcgc agggcttttt tatttgttta tgaagtatgc cttcggggtc ctgtccccgt
     1381 tcataatagc catgtttata gccatgctgc ttcaaaggcc cgtaaatttc gtgattgaaa
     1441 agacgaaaat acctcgcggt atagtttcga ccgtggcggt tttgctcgtc gtgtttgttt
     1501 tcggaacaat aataggactt atactttcca aaatattttc cgagctgaag tcctttttta
     1561 attatattat gctgcagctt gacaatgcgc cgctgtttgc gtcgcagctc aaggattggg
     1621 tcaacactca ctttacgttg ctgccggact ctataaggca gaatattatc tcgaccgtgg
     1681 cgaatcttat agataaaata cagggcgtgg atacgggcag ctcttcttcc gcggcggcag
     1741 ccgtagcgtc aagcgagacg gcggcaaagg gcttcgattt ttcgtcattc tcatcaccga
     1801 tgagcgctct ttggggaacg gcaaagcaaa taccgtctat actcgtcggc accatagtcg
     1861 gcatagtttg ctgctgcttt atgacttccg attatcacac acttcgctcg ctgattatga
     1921 ctcaggcggg caagcgcgcg gacgccttta taagaacaaa gcaaataata ttttcaacgc
     1981 tcggaaagat gataagggca tacggcctta tcatgtgcat aaccttcctt gaaatggtgc
     2041 tcggcctttc gatattaaag ctgttcggaa tatatgaaaa cggctatatt tttgcgattg
     2101 cgcttataac cgccgtgata gatattgttc ccgttctcgg cacaggcacg gtgctgatac
     2161 cgtgggcaat ttggatgttc ttctccggcg atgttgccat gggcataggg cttcttgtga
     2221 tttacggcgt tataaccgtt gtacgccaga tagtcgagcc gaagcttgtg gcgtcgcagc
     2281 tcgggcttcc cgcatttgtc actcttatgg cgatgtatat aggctcgcag ctgttcggat
     2341 ttgtcggact gttcctgctt ccgatgacgg ttatgctcct taaggtgctc aatgacgacg
     2401 gcgtaattca catattcaag gttccgcccg aggaggcggc ggaatttaag gctgccaagg
     2461 acaaggacga gaaaacggac gccgagagga aggctaaaaa gcgcgaaggc atcaagaaaa
     2521 tcaaaacagg aaagaagaag taatcgagaa cccgatatga agatgattga tttgcactgt
     2581 catatcctat atgacatcga cgacggcgca aaaacaaaag aggattccgc cgcgctgctt
     2641 cacacggcgg cacaaaacga aataaaagcg ataatagcaa cgccgcattt taatgattat
     2701 tcggcggtgg acgagtttgt cgcaaaacga gacgaaagag taaaatttct gcgtgaaatt
     2761 ataggcggaa aagggcttga tataggcctc ggagccggcg cggaggtctt tttgcaaaac
     2821 gatgtttttt cggactgcga tttgtcgccg ctctgcataa acggcagccg ctatttgctg
     2881 tgcgaatata cgctgcgtcc gtttgaccca aaatacgccg tcatatacgc cgagcgtgtt
     2941 ttatccatgg gacttgtacc gataatagcg catccggaaa ggtacatatg tttccacacg
     3001 gagaagtggg tcgcaaagga gctttgcgat atgggtgctc ttttacaggt taacgcttcg
     3061 agccttgctg gcagaggcgg cgatgaaata aaggattatg cgacggagct cgtgctttca
     3121 aggcttgccg attttgttgc cacggatgcg cattcggcgg tatcaagacc caatgatatt
     3181 ataaaatgcg cggagcgttt tgatgagaga ataacttcgg aggacataaa gagattggtt
     3241 tacaaaaacc cgttgaaggt tctgaaggat gaaatattgt agtcgaagca caaatatgta
     3301 ttttaatgag ggcgattatg gaaaaaataa aacggcgtgg tgaaaattac accataccgc
     3361 agatagattt aagcgttgaa ccgaaaaaca caaaagagga atttaaatat ctttacgaaa
     3421 atgtcaccgt tgagaaaaag ggcggttatc tcgggcaccc cgattccgtg cttttaaagg
     3481 acgggagtat actcgtcttt ttcccggagg gtcacggcaa gggaaaaact ctttcaaaaa
     3541 tcagccgcga cggcggcaga agctataccg aggaaatcaa aaatccgccg aaaagctggg
     3601 aaaattcgcg cgagactccg acggtttatc gtcttgagtt ctccgagagc gacacacccg
     3661 acaagctgat aatgataagc ggaaacccca tatg
//
LOCUS       DBMDVO010000051         3666 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11106_length_3666_cov_1.149790, whole genome shotgun sequence.
ACCESSION   DBMDVO010000051 DBMDVO010000000
VERSION     DBMDVO010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11106_length_3666_cov_1.149790"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="ACZPE2_01320"
     CDS             <1..487
                     /locus_tag="ACZPE2_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1318518.1"
                     /translation="GVKQANMEEEVLTSFPDGKTAKEFHFAKGIMEADVIINVCKMKT
                     HALERVTGAVKNVYGFICGAYMPVSSFSDGLQKVISFLPGTYGTGLLRNHALNGVFSE
                     LSETGMPKEFVTVFKDSMDCNLYFFDKCVSIEAMYAVLCCSVVVLVLAYIILNVAVKK
                     R"
     gene            complement(573..1769)
                     /locus_tag="ACZPE2_01325"
     CDS             complement(573..1769)
                     /locus_tag="ACZPE2_01325"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1318519.1"
                     /translation="MSVVNALVEIFQSSGFVSLSWQNWVMILVSFVLLYLAIVKKFEP
                     LLLVPIAFGMLLVNIYPDIMYDPLMMSSYSGQVVEGAHWYDTGVLRLIYAGVKSSIFP
                     CLIFMGVGAMTDFGPLLANPSSLLLGAAAQLGIYVAFLISIATNLIPGFEGFTPQEAA
                     SIAIIGGADGPTAIYLTSKLAPQLLGPIAVAAYSYMALIPLIQPPIMKLLTTKKEREI
                     KMDQLRTVSKTEKIIFPIVVTVLCVLVLPSVAPLIGMFMLGNLFKECGVTERLSDTAQ
                     NSLCNIVTIMLGTTVGATAEGKTFLATKTILVIVIGLVAFAFSTAGGVLFGKLMNLVT
                     HGKVNPLIGSAGVSAVPMAARVSQVEGRKYNPSNFLLMHAMGPNVAGVIGSAVAAGYL
                     LLTFGK"
     gene            complement(1782..2165)
                     /locus_tag="ACZPE2_01330"
     CDS             complement(1782..2165)
                     /locus_tag="ACZPE2_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR1318520.1"
                     /translation="MKYVVTLNGKDYEVEVDELSEAIVTNVAPTAASAPAAPVATQPS
                     APATPAPAPVSGSGTAVKAPMPGTILKVNVTAGQSVKAGDIMFILEAMKMENEIVAPS
                     DGTVSQVVAAKGSSVATDDVLAYLN"
     gene            complement(2178..2540)
                     /locus_tag="ACZPE2_01335"
     CDS             complement(2178..2540)
                     /locus_tag="ACZPE2_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016187.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1318521.1"
                     /translation="MYLLDSVMSIGEALNVSITGIVVVFIVLVVLAVLVQLLSKLIRL
                     FVKEPNAKNAKKSDAVKEVVKPQGGVALPENQTLGTLDLYKTDEKTAAVIMAIVSKES
                     GVPLNRLKFNSIKLIENK"
     gene            complement(2552..>2996)
                     /locus_tag="ACZPE2_01340"
     CDS             complement(2552..>2996)
                     /locus_tag="ACZPE2_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402406.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HIR1318522.1"
                     /translation="QNKMDKFNDVLLETPKVRKDLGYPPLVTPMSQMVGVQATVNVLV
                     GERYKNISKEVKAYIKGEYGRAPGEIDKDLMKKVLGDEEPIKGRFADSLEPGFEKAKK
                     EIGEKAKSDEDVLSYIAFPTQAEAFFDKREEKEKNTFKYSIKRID"
     assembly_gap    2997..3006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3007..>3666)
                     /locus_tag="ACZPE2_01345"
     CDS             complement(<3007..>3666)
                     /locus_tag="ACZPE2_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402406.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HIR1318523.1"
                     /translation="LQMLLRGQNLLGYHHYPDDVVRMFVKKSIENGIDIIRIFDALND
                     VRNIEVAVDETLKNGAIASGAICYTQSPIHNLESYIKLAKQMEELGCQTICIKDMAGI
                     LGPKEAYDMVKALKENVKTPIILHTHCTTGLGMLTLQKAVEAGCDVIDTAISSFSGGT
                     SQAPTETMAYALKQEGYDIDLDFSVLKDINDFFKPIKDEYLKKGSLNPLVLSTDTNAL
                     NY"
BASE COUNT         1091 a          750 c          755 g         1060 t
ORIGIN      
        1 cggagtgaaa caggccaata tggaagaaga agtattgact tcatttccag acggaaaaac
       61 agcaaaggaa ttccattttg caaagggcat catggaagca gatgtcatca tcaatgtctg
      121 taagatgaaa actcacgcat tggagcgggt gacaggagct gtaaagaatg tgtatggatt
      181 tatctgtggc gcatatatgc ccgtctcaag cttttctgac ggactgcaaa aagttatttc
      241 ttttttgccg ggtacatacg gcacgggact tttacgcaat cacgctttaa atggtgtttt
      301 cagtgaacta tccgaaacgg gtatgccgaa ggaatttgtc accgtattta aagacagtat
      361 ggactgtaac ttgtactttt tcgataaatg cgtcagtata gaagcgatgt atgctgttct
      421 gtgttgttcg gtggttgtgc tggtgttagc atatattata ttaaatgttg cagtcaagaa
      481 aagataaata agttttcaat tgttatatca gaatatataa aaaggtcacg gtaactaaat
      541 ttgttaccgt gaccttttct ataacttaat aattatttac caaaagtaag aagtaaatat
      601 ccggctgcaa cagcagaacc gataactcct gcaacattcg gacccatagc gtgcattaaa
      661 aggaagttag agggattgta ttttcttccc tcaacctgag atacacgagc cgccatagga
      721 acagccgaaa cacctgcgga acctataagt ggattaactt taccatgagt tacgaggttc
      781 ataagtttgc cgaacaatac gccgcctgcg gttgagaaag caaatgcgac aagaccgatt
      841 actattacaa gtatagtttt ggtagcgagg aatgttttgc cttctgctgt ggcgcctacg
      901 gtggtaccga gcattattgt tacgatattg caaagagaat tctgagcagt atcactgagg
      961 cgttcggtta cgccgcattc tttgaaaaga ttgccgagca taaacatacc gataagcgga
     1021 gcgaccgacg gaagaacaag tacgcaaaga acagtaacaa caatcgggaa aatgattttt
     1081 tctgttttgc ttacggttct aagctgatcc atcttgattt cgcgctcttt ttttgtagtt
     1141 aaaagcttca taataggggg ctgaatcagc ggtataagag ccatataaga gtatgccgct
     1201 acagcaatgg gtcctaatag ctgcggagcg agcttactcg ttaaataaat agctgtcggt
     1261 ccgtctgcac cgccgattat agctattgaa gctgcctcct gaggcgtaaa gccctcaaaa
     1321 ccggggataa ggttggttgc gattgaaata agaaaagcaa cataaatacc aagctgagca
     1381 gcagcgccga gaagaagact tgaaggattc gcaagaagcg gtccgaagtc ggtcatagca
     1441 cctacaccca taaagataag gcaggggaat atactcgatt taacaccagc gtaaatcagt
     1501 cgcaagacgc cggtatcata ccaatgggca ccttcaacaa cttggccgct gtaagacgac
     1561 atcataagcg gatcatacat aatatcagga tatatgttaa caagtaacat accgaaagca
     1621 attggaacta agagaagcgg ttcaaatttt ttgacaatag ccaaatataa aagaacaaag
     1681 gagactaaaa tcataaccca gttttgccat gacaacgata caaaaccgga gctttgaaat
     1741 atctccacca aagcatttac aacagacatt tattgacctc ctcagttcaa ataagcaagg
     1801 acgtcatcag ttgcaaccga gctgcctttt gcggctacaa cctgtgaaac tgtaccatcc
     1861 gagggagcta caatttcgtt ttccatcttc attgcttcaa gaataaacat aatgtcgccg
     1921 gccttaacgc tctgacctgc ggtaacattt actttaagta ttgttccggg catgggagcc
     1981 ttaacagcgg ttccgcttcc tgaaacagga gcaggtgcgg gagtagcagg tgccgagggc
     2041 tgtgtagcca caggtgctgc cggagcagaa gctgcggtag gcgcaacgtt tgttactatt
     2101 gcctcactta actcgtcaac ttcgacttca tagtcttttc cgtttaaagt aactacatat
     2161 ttcatattgt taacccctta cttgttttct ataagcttaa ttgaattgaa ttttaatctg
     2221 ttcaaaggaa ctccgctttc tttgctgact attgccataa ttacagccgc agtcttttca
     2281 tcggttttat aaagatccaa cgttcccaat gtttgatttt ccggaagagc aacaccgcct
     2341 tggggtttta caacctcttt aacagcatcg gatttttttg cattctttgc attcggctcc
     2401 tttacaaaca atctgatgag cttagaaaga agctgaacaa gaactgccaa aacaacaagg
     2461 acaatgaaca caactacaat gccggttatt gaaacattaa gagcttcacc tatggacata
     2521 acactgtcta ataaatacat cttaacaccc cttagtcaat tctttttatt gaatatttaa
     2581 aagtattctt ttctttttct tcgcgtttat caaaaaatgc ttcagcttga gtcgggaatg
     2641 caatataaga aagaacatct tcgtcgctct ttgccttttc tccgatttct tttttagcct
     2701 tttcaaaacc gggttcgaga gagtcggcaa atctgccctt aataggttct tcgtcgccga
     2761 gaaccttttt cataaggtcc ttatcgattt cgccgggggc acgaccgtat tcacccttaa
     2821 tataagcttt tacttctttg gaaatattct tatatctctc tccgacaaga acgttaacag
     2881 ttgcctgaac gccgaccatc tgactcatag gtgttacaag cgggggataa ccgagatcct
     2941 ttctaacctt gggggtttca aggagaacat cattaaactt atccattttg ttctgtnnnn
     3001 nnnnnnataa ttgagtgcat ttgtgtcggt tgaaagcaca agaggattta aagaaccttt
     3061 ttttaaatat tcatctttta taggcttaaa gaagtcgttg atatctttaa gaacagaaaa
     3121 atcgaggtca atatcataac cttcctgttt taaagcataa gccatagttt ccgtaggagc
     3181 ctgtgaagtg ccaccggaga atgaggatat agcagtatcg attacatcac atcctgcttc
     3241 aacggctttc tgaagagtaa gcataccgag tcctgtggta cagtgtgtat gaagaataat
     3301 gggagttttt acattttcct taagagcctt taccatatca taagcttctt tcggacctaa
     3361 aatgcctgcc atatccttaa tgcatatagt ctggcaaccg agctcttcca tctgcttggc
     3421 aagcttgatg tatgattcaa gattgtgtat tgggctttga gtatagcaaa ttgcgccgga
     3481 cgcaatggca ccattcttta aagtctcgtc aaccgcaact tctatatttc taacgtcgtt
     3541 aagagcatcg aatattctta tgatatcaat gccgttttca attgattttt taacgaacat
     3601 tcttactaca tcatcgggat aatggtggta tccgagcaaa ttttgacctc ttaaaagcat
     3661 ttgaag
//
LOCUS       DBMDVO010000052         3664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11116_length_3664_cov_1.112265, whole genome shotgun sequence.
ACCESSION   DBMDVO010000052 DBMDVO010000000
VERSION     DBMDVO010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11116_length_3664_cov_1.112265"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(406..723)
                     /locus_tag="ACZPE2_01350"
     CDS             complement(406..723)
                     /locus_tag="ACZPE2_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1318524.1"
                     /translation="MSDIIKSAEKEFDYIIIDTAPCGVVADSSILSSYADGIILVVRN
                     DMVASRRIKRAVENLENSGTKLVGCIYNDTESGMPKRLMSSYNKKYGYGYGYGYGYGS
                     NQK"
     gene            complement(745..1872)
                     /locus_tag="ACZPE2_01355"
     CDS             complement(745..1872)
                     /locus_tag="ACZPE2_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014732.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1318525.1"
                     /translation="MAYSSSGKTHAEESVNLDSVVIYLKKILQHWKIVVISAVVLAVL
                     GFAVAKLTYTPSYSSKITFIANNKSSALAVSGQTQSDLNASATLAESFKYVFTTTELS
                     KSVADNSGFKDISVSDIKKWVSIEAVEDTAIINLTVTTPNADVSYGIANAYVNNYESA
                     INAAFPSTTLTVIDPPLLASAPDRDASAKIYTIVGFAAGLVISIFAIVLAVIAKDTVK
                     TADDVKDKLGVDVIGMVTHVNRKKKKKGEDYKPLLISDRRSGFPFIETFKIIRTKIEH
                     TAQREKYKTFVVTSTAENEGKTTSAVNIALALAQNGKSVLLVDGDLRKPAVAKLLGIN
                     ASEDGGVYGVINGEKTLAEAIKYSEKYNLFLLVSGKPVFDP"
     gene            complement(1962..3629)
                     /locus_tag="ACZPE2_01360"
     CDS             complement(1962..3629)
                     /locus_tag="ACZPE2_01360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318526.1"
                     /translation="MKKSSAEKWIASHSKKESPRMVLLIIGNALFSVCGVMTALYAKY
                     IIDAAQNADKNALIKNGVALGALILLQTFLKVVCKALEARVQGKIEISIKTELFSSIL
                     KKDYEKITSIHTGELMTRLTSDVSVVSDGVVTILPSAVAMVTRLVCAFAALFSLDRMF
                     SLILLSGGALLFVFTRFFRGKMKSLHKAVQKTDGGVRSFMQEVIENLLVVKIFDVADM
                     MKDRSKVYQDENYYAKIKRNRWSIFANIGFSAAFSVGWLFAMLWSAARLSRGAITFGT
                     LTAIVQLVSQVQTPVTSLSGYLPKYYTMLASADRILETESLPESVAYNMPGLDVGKIY
                     DEMSYMEMESVTFGYGRENVIENGSVRINKGDFTVISGISGIGKSTFFKLLTGVLTPQ
                     KGSISVVSGGKKYHIDKYMRPLFAYVPQGNMLMSGTIRDNIKLTNSTATDDEVLEAAK
                     ISCAYDFINELPSGLDTVLMENGRGLSEGQIQRLAVARAVLSGAPVMLLDEATSALDA
                     ETEKKLLLGLRSLKDRTCVIISHKTAAFEVADSVIEIKDGHLHSKKI"
BASE COUNT          916 a          912 c          798 g         1038 t
ORIGIN      
        1 acggtcgcac accacttcgt ttttaatgaa ggtgagactt ttgatttggg aaattttttc
       61 cggtaacgca gtaagaccgg ctctcacgat ttcgatgttt tcgtcaagcg ttttgccctc
      121 tgcatcacag aatttccaca ttacgttgtg ataaagcatt ttctcacctc cgaaaagccg
      181 ttacaagctg taaccgcata attattatat aattttaaca tagaaaatct taatgggcaa
      241 tagctttttt agtaaaaaca ttgctaaagc agcgtttttt tgagcgtata cgcagttctg
      301 catcgcttta tgccgaaaaa gactgacggt gagtcttagc cgagctttcg gcaaaactaa
      361 aaaagcggag attcgccgca gcgaacctcc gtaaaacgag cttatttatt tttgattgct
      421 gccgtagccg taaccgtatc cgtaaccgta gccgtacttt ttgttgtagg acgacataag
      481 gcgtttcggc ataccgctct ccgtatcatt gtagatacat ccgacgagct tggtgccgga
      541 attttcgagg ttttcaacgg cgcgcttaat acgtcttgaa gcaaccatat cgtttctgac
      601 aacaagaatt attccgtcgg catagcttga aagtatcgac gaatccgcca caactccgca
      661 gggagccgta tcaattataa tataatcaaa ctccttttcc gcggatttga ttatatcgga
      721 catagcgtcc gtagagagaa tctcctacgg gtcaaatacg ggctttccgc tgacaagcag
      781 gaagagattg tatttttccg aatactttat cgcctcggcg agagtctttt cgccgtttat
      841 gacaccgtat acgccgccgt cctcggaggc attgattccg agaagctttg caactgcggg
      901 cttacgcaag tcgccatcca ccaaaagcac cgacttgccg ttttgtgcga gtgcaagtgc
      961 gatgtttacc gcgcttgtcg tcttgccctc gttttcggct gtactcgtta ccacaaaagt
     1021 tttgtacttc tcacgctgag cggtgtgctc gatctttgtt cttataatct taaaggtttc
     1081 gatgaacggg aagccgctgc gtctgtcgga gataagcaga ggcttataat cctcaccctt
     1141 tttcttcttt ttacggttaa cgtgagtgac cataccgata acgtcaacgc cgagcttgtc
     1201 cttaacatca tcggcggttt taaccgtatc cttggcaatt accgcgagaa caatcgcaaa
     1261 tatcgatatg acgagacctg cggcaaagcc tactatagta tagatttttg cgctcgcatc
     1321 gcgatccgga gcactcgcaa gaagcggcgg gtcgataacc gtcaaggtgg tactcgggaa
     1381 agccgcgttt atggcgctct catagttatt tacataggca ttcgctattc cgtatgaaac
     1441 gtcggcattc ggcgtagtca cggtaagatt tattatagcc gtgtcttcaa cggcctcaat
     1501 cgaaacccat ttctttatgt ctgaaacgga aatatcttta aacccgctgt tgtcggcaac
     1561 gctctttgaa agctctgtcg ttgtaaaaac atatttgaag ctctcggcaa gcgttgcgga
     1621 cgcgttaagg tccgactgag tctgacccga aacggcgagt gccgaggatt tattgtttgc
     1681 tataaacgtt atcttagacg aatatgacgg cgtgtaggtg agctttgcaa cggcaaagcc
     1741 gagcacagcc agcactactg ccgagataac aactattttc caatgctgca atattttttt
     1801 gagatatata acgacgctgt cgagattgac gctctcctca gcatgagttt tccccgaaga
     1861 tgaatatgcc atctttttaa ctccttttaa gcttaattaa cgcaaaacaa ttatttgtat
     1921 tatatcaaat tcgtcccgat actacaatag aaaaaataaa tttatatttt ttttgaatgc
     1981 aaatgtccgt cttttatctc tataacgctg tcggcaactt caaaagccgc ggttttgtga
     2041 gagataataa cgcacgttct gtcttttaac gacctcaagc ccaaaaggag ctttttctcc
     2101 gtttcggcgt caagcgcgga cgtcgcttcg tcaagcagca taacaggcgc gcccgacagc
     2161 acagcccttg caactgcgag gcgctgaatc tgcccctccg acagtcccct gccgttttcc
     2221 ataaggacgg tatcaaggcc gctcggcagc tcgtttataa aatcataggc gcagcttatt
     2281 tttgccgctt caagcacctc atcgtccgtc gcggtgctgt ttgtgagctt tatgttatcg
     2341 cgtatggtgc cgctcatcag catattcccc tgcggaacat atgcaaacag cggacgcata
     2401 tatttgtcaa tatgatattt tttgccgccg gacacaacac ttatacttcc cttttgcggt
     2461 gtaagcaccc ctgtaagcag cttgaagaat gtgcttttcc cgatgcccga tattcccgaa
     2521 atcacggtga aatcgccctt gtttatccgc acagagccgt tctctattac attttctctg
     2581 ccgtagccga aggtcacgct ctccatttcc atatatgaca tctcatcata tattttgccg
     2641 acgtcaagcc ccggcatatt atatgcgacg ctctcgggga gcgactcggt ttcaagtatc
     2701 ctgtcggcgg aggcgagcat ggtatagtat ttcggcaggt aacccgacag actcgtaacg
     2761 ggcgtttgca cctgactcac aagctgaact atcgccgtga gagttccgaa ggttatcgct
     2821 ccgcggctca gccttgcggc actccaaagc atcgcgaaaa gccagcccac cgaaaatgcc
     2881 gccgaaaagc ctatatttgc gaaaatcgac cacctgttgc gttttatttt agcatagtaa
     2941 ttttcgtcct gatacacctt cgagcggtct ttcatcatat cggcaacgtc aaatatctta
     3001 acgacgagca gattttctat tacctcctgc ataaaggagc ggactccgcc gtcggttttt
     3061 tgcaccgcct tgtgaagaga cttcattttg ccgcgaaaga atcgggtgaa tacaaacagc
     3121 aaagctccgc ctgacagcaa tatgagtgaa aacatcctgt caagcgaaaa aagagccgca
     3181 aaggcgcaaa cgagcctcgt caccatagca acggcggacg gcaatatcgt tacaacgccg
     3241 tcggaaacca ccgagacgtc acttgtgagc cttgtcataa gctcgcccgt gtgaatcgag
     3301 gtgatttttt cgtagtcctt tttgagtatt gatgaaaaca gctctgtttt aatgcttatc
     3361 tcgattttgc cctgtactct cgcctcaagt gctttgcaaa caaccttgag aaatgtctgt
     3421 aaaagtatga gcgcgcccaa agcgacgccg tttttaatca gagcgttttt atcggcattt
     3481 tgcgccgcgt ctattatata cttcgcatac agcgccgtca taactccgca cacggaaaac
     3541 agggcgttgc cgattatcag cagcaccata cgagggctct ctttttttga gtgagaggct
     3601 atccattttt cggcagagct ttttttcact taccgagctt cccccttatt tttactatat
     3661 attt
//
LOCUS       DBMDVO010000053         3662 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11125_length_3662_cov_1.234766, whole genome shotgun sequence.
ACCESSION   DBMDVO010000053 DBMDVO010000000
VERSION     DBMDVO010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_3662_cov_1.234766"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>407
                     /locus_tag="ACZPE2_01365"
     CDS             <1..>407
                     /locus_tag="ACZPE2_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1318527.1"
                     /translation="DHDVNGRLKVVYIEDYNVTMAEKLMPAADISEQISLAGTEASGT
                     GNMKLMLNGAITLGTMDGANIEIHDAVGSDNIFIFGMKTPEVNELKRRGYNPMNYYNN
                     NQELHNVIDFIAKNDIAGKRFPEIANIINNDPY"
     assembly_gap    408..417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <418..883
                     /locus_tag="ACZPE2_01370"
     CDS             <418..883
                     /locus_tag="ACZPE2_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1318528.1"
                     /translation="ALDMLSAGICNQQFPDIANSLRFNDPYMVLADFADYRRMQQYAV
                     SVWADKKKWQQMSLMNISGAGIFSADRAIHEYADNIWHTKSAFNGETYPIAEPAKVKK
                     EAAEQITEKKAAEAKAPEKKAENKPAEKKPTEKKAAAKTAASAGKKAKKKKK"
     gene            complement(978..1235)
                     /locus_tag="ACZPE2_01375"
     CDS             complement(978..1235)
                     /locus_tag="ACZPE2_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1318529.1"
                     /translation="MMYSYKTKGTCSRQIDVELDGDVIKDVKFYGGCNGNLQGISAIV
                     RGKTVEEVEKAFSGIKCGFKNSSCPDQLVCALKEAKAHEGK"
     gene            complement(1232..2101)
                     /gene="rsmA"
                     /locus_tag="ACZPE2_01380"
     CDS             complement(1232..2101)
                     /gene="rsmA"
                     /locus_tag="ACZPE2_01380"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1318530.1"
                     /translation="MSVDLADINIVKSVLARHGFAFSKSLGQNFLIDSSVCPRMADAA
                     ATDKSYGVLEVGPGIGVLTSELSKRAKKVVSVELDKRLLPVLHETLCDCNNVEIVNDD
                     ILKLDLKKLISEKLDDCSEVTVCANLPYYITSPVIMKLLSEQLPLKKIVVMVQKEAAD
                     RFCAEVGGKNSGAVTVGVNYYADARLLFPVPRESFIPSPKVDSAVIELTLLDGKRVTP
                     KSEKVFFNTVKAAFSMRRKTAVNGLSGGLCISKEAAAAAIERAGLSPTVRAEKLSMEE
                     LSRLSDEIGKELK"
     gene            complement(2091..3278)
                     /locus_tag="ACZPE2_01385"
     CDS             complement(2091..3278)
                     /locus_tag="ACZPE2_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR1318531.1"
                     /translation="MEYVFSDRISALQPSAIREILKATADPAIIPFAAGNPDVAAFPV
                     DDVRRISAKIFENEPITALQYGVTEGYEPLRKTVADYMKKKNNIGRDFDKLIITSGAT
                     QVMDLATKSLCNFGDIIVCENPSFIGSLNCFRSYGCKLKGVPVEEDGMDMNALEKVLK
                     ENKNVRFIYTIPNFQNPSGRTMSLEKRKTLYALAKKYGVLILEDNPYGDLRVSGENVP
                     TVKSMDEDGIVIYAGSFSKVLAPGIRVAYVIAPEPIVAKMTVCKQGQDVHTAMFNQML
                     VYEWMSTTDFEAHIGKIRDIYRRKMTLMCDLIDEKLGDLVSYVRPEGGMFVWCELPEK
                     IDMLDFVKRAIKEKVAVVPGTAFMVEDGDTTNCFRMNYTTPSDENIVLGMERLEKVKK
                     DVC"
     gene            complement(3435..>3662)
                     /locus_tag="ACZPE2_01390"
     CDS             complement(3435..>3662)
                     /locus_tag="ACZPE2_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HIR1318532.1"
                     /translation="TVWSFSGFTYEELTGDSRAVCEVTKEMLSMLDVLVDGEFVEAKR
                     NISLRFRGSENQRLIDMNKTRKEGKIVLWDK"
BASE COUNT         1001 a          949 c          761 g          941 t
ORIGIN      
        1 cgaccacgac gtaaacggca gactcaaggt tgtttacatt gaggattata acgtaacaat
       61 ggccgaaaag cttatgccgg ccgccgacat ttcggagcag atttctcttg ccggcacaga
      121 ggcgagcggt acgggcaata tgaagcttat gctcaacggc gccataaccc tcggcacaat
      181 ggacggtgca aacattgaaa tccatgacgc cgtaggcagc gacaacatct ttattttcgg
      241 catgaaaact ccggaggtaa acgagctcaa gcgccgcggc tacaacccga tgaattacta
      301 caacaacaat caagagctgc ataacgtcat tgacttcatc gcgaaaaacg atattgcagg
      361 aaagcgtttc cccgaaattg ccaacatcat caacaatgac ccgtatannn nnnnnnncgc
      421 gcttgatatg ctctctgcgg gtatctgcaa ccagcaattc cccgacatcg ccaactccct
      481 gcgctttaac gacccgtata tggttcttgc cgattttgcc gattacagac gtatgcagca
      541 gtacgccgtt tccgtttggg cggataagaa aaagtggcag cagatgtcgc ttatgaacat
      601 ctccggtgca ggcattttct cggcagacag ggcaatacac gaatatgccg ataacatttg
      661 gcatacaaaa tccgcattca acggcgagac atacccgata gctgagcctg caaaggtaaa
      721 aaaagaggca gccgaacaaa tcacggagaa aaaagctgca gaggcgaaag cccctgaaaa
      781 aaaagccgaa aataaaccgg cagaaaaaaa gccgacagaa aaaaaggcag ccgcaaaaac
      841 agccgcttcc gcaggcaaaa aagctaagaa aaagaaaaaa taaaatcgca gcagcataaa
      901 cgtaaaggcg tcatcaaaat tttgatgacg cctttttatt ataatacatt gctaatacat
      961 tgcggctttt cagcggctta tttgccttcg tgagccttag cctccttcag cgcacagaca
     1021 agctggtcgg gacaagatga gtttttaaac ccacacttga ttcccgagaa agccttttcg
     1081 acctcttcca cggttttacc gcgaactatc gccgaaatac cctgaagatt tccgttacat
     1141 ccgccgtaga acttaacatc cttgataaca tctccgtcaa gctccacatc tatttgcctt
     1201 gagcaggttc ccttggtttt atagctatac atcattttag ttcctttcct atttcatccg
     1261 agagccgtga aagctcctcc attgagagct tttccgctct caccgtaggc gacagccctg
     1321 cacgctctat tgccgccgcc gctgcctcct ttgatatgca aagtccgccc gacaagccgt
     1381 ttacagctgt tttgcgacgc attgaaaatg ccgctttgac cgtattgaaa aacacttttt
     1441 cgctcttagg agtcaccctt ttgccgtcaa gaagcgtcaa ttcaatcacc gcgctgtcaa
     1501 ccttcggact cggtataaag ctctctctcg ggacgggaaa caagagcctt gcatcggcat
     1561 aataattcac gcctaccgtt accgcgccgg aatttttacc gccgacttcg gcacagaatc
     1621 tgtctgccgc ctctttttga accataacca ctattttttt aagcggcagc tgctcggaca
     1681 gcagcttcat aattacgggt gaagtgatat aatacggcaa atttgcacac accgtcactt
     1741 cgctgcaatc gtcaagcttc tcggatataa gcttttttaa atcaagcttt aaaatatcgt
     1801 cgtttactat ttccacatta ttgcagtcac aaagcgtttc gtgaagcacc ggcaagagcc
     1861 gcttgtcaag ctctaccgaa acgacctttt tagcgcgctt tgaaagctcg ctcgtcagca
     1921 cccctatgcc gggtccaact tcaagcactc cgtaagactt gtctgttgcc gcggcgtctg
     1981 ccatacgcgg acaaacgctg ctgtcgatta aaaagttttg cccgagagat ttggaaaacg
     2041 caaagccatg ccgtgcgaga acagatttta caatatttat atcagctaaa tcaacagaca
     2101 tcttttttaa ccttttcgag tctttccata ccgagtacga tattttcatc tgagggtgtg
     2161 gtgtaattca tacggaaaca attagtggtg tcgccgtcct ccaccataaa tgcagtaccg
     2221 ggaacaacag ccaccttttc cttaatggct cgctttacaa agtcaagcat atcgattttt
     2281 tcgggaagct cgcaccagac gaacattccg ccctcggggc gtacatacga tacaaggtcg
     2341 ccgagctttt catctataag gtcacacata agagtcatct tacggcggta aatatcgcgg
     2401 attttgccta tatgcgcttc aaaatcggta gtgctcatcc actcgtatac aagcatctga
     2461 ttaaacattg ccgtgtgaac atcctgcccc tgcttgcaga ctgtcatctt tgcgacaatc
     2521 ggctccggag ctattacgta agcaacgcgg atacccggtg ccaacacctt tgagaaggag
     2581 ccggcatata ttacgatacc gtcctcatcc atgctcttga cggtaggtac attttcaccc
     2641 gatacacgca aatcgccgta tgggttgtcc tctaaaatta acacgccgta ttttttggca
     2701 agtgcataca aggtctttct tttttcgagc gacatagttc tgccggacgg attttggaaa
     2761 ttaggtatgg tgtaaataaa acgcacgttc ttattctctt taagaacctt ttcaagagcg
     2821 ttcatatcca taccgtcctc ttcaaccgga acgcccttaa gcttgcagcc gtaagagcgg
     2881 aagcagttaa gcgagccgat aaacgacgga ttttcacata cgattatatc gccgaaatta
     2941 cagagtgatt tcgttgccaa atccataacc tgagttgcgc ccgaggttat gataagctta
     3001 tcgaagtctc ttcctatatt gttctttttc ttcatataat cggcaacggt ttttctgagc
     3061 ggctcatagc cctcggtaac gccgtactga agagccgtta taggctcgtt ctcaaaaatt
     3121 tttgcgctga tacgtcttac gtcgtctacg gggaacgccg cgacgtcggg attgcctgcg
     3181 gcaaacggga ttatcgcagg gtctgccgta gcctttaata tttcacggat tgcggacggc
     3241 tgaagagccg aaattctgtc cgaaaataca tattccataa tattttgcat ctccttgggt
     3301 gatagtgacc ttacacataa agtacaacaa tgattataac acaacgctcc gaatataaca
     3361 agcgcggcgc atactgtttc agatataccg aaaagacaaa acggatgcaa aggaagcctt
     3421 cacacccgta gcttttattt gtcccacagg actattttgc cctcttttcg cgttttgttc
     3481 atatcaataa gtctctgatt ttcacttccg cgaaatctca atgaaatatt tcttttggcc
     3541 tctacgaatt cgccgtcaac aagcacatcg agcatagaca gcatctcctt tgtaacctcg
     3601 cacacggcgc ggctgtcgcc cgtcaactct tcataggtga accctgaaaa gctccacacg
     3661 gt
//
LOCUS       DBMDVO010000054         3660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11138_length_3660_cov_1.160438, whole genome shotgun sequence.
ACCESSION   DBMDVO010000054 DBMDVO010000000
VERSION     DBMDVO010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11138_length_3660_cov_1.160438"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="ACZPE2_01395"
     CDS             <1..569
                     /locus_tag="ACZPE2_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1318533.1"
                     /translation="RYKGHDYLVNRFPGIYEGCLKHGVDMTKDLIPVYPCQHYLMGGI
                     DVDLDARTSISRMYAVGECSHTGVHGKNRLASNSLLEALVFGKRAADDIASLSKKDPD
                     HVTVTEHKTDISGAPLPKGMRTEIRSIMQRSYFVIPDMDAVRVGLKRVDTILMRLKNG
                     KFAITPDYCEALSLATVAHIILKEVDEG"
     gene            566..1405
                     /gene="nadC"
                     /locus_tag="ACZPE2_01400"
     CDS             566..1405
                     /gene="nadC"
                     /locus_tag="ACZPE2_01400"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR1318534.1"
                     /translation="MILPQFYTDDIIKRAILEDINYVDVASDYLISEEQRNEAYFVSK
                     ADGVLCGIDVAMRVFELLDDSFTAKVYMHDGDKVKKGDLIAEFSGKTTLLLKGERTAL
                     NLLQHMSGIATATAKAVKLCEGTSASIADTRKTLPSLRALQKYAVTCGGGKNHRFNLS
                     DCAMLKDNHIDAGGGITGAVKKLREKIGHTVKIEVETRNLDEVKEAVKAGADIIMLDN
                     MDCDTMKKAVEIIGGKALTEASGGITDETIPGVAACGVDIISIGALTHSVQAFDISMK
                     MKK"
     gene            complement(1568..2350)
                     /locus_tag="ACZPE2_01405"
     CDS             complement(1568..2350)
                     /locus_tag="ACZPE2_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318535.1"
                     /translation="MFGVIKIERIGGGFFKRLHYRLFPPEPVIERISVLGSAPFFTLT
                     LVCDENEEVDTGKIYSLLGRCAGRVIVCGGTITEDEKVKNFEPRILPSVMLFNSAVDY
                     IKKCSLPPEKTSVAVMDFNGFQKDKLSLLVPLASNLKVITGNPEEFSPVRRRLYDDWG
                     LAMTVTENVNEAGGCTFVIAPRTDKSNPDGGCLLYRNEYSRSTVCLSGSGVTLPDDIT
                     KVLPPCNSLLFASALYELCGATRLSELCFDRLADISSKGRIE"
     gene            complement(2404..3624)
                     /locus_tag="ACZPE2_01410"
     CDS             complement(2404..3624)
                     /locus_tag="ACZPE2_01410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1318536.1"
                     /translation="MNRKISLGLALALIFVAMAGSVAGTMAVSMKMYNSIIKDLPKRS
                     QTYSLLAEIDDVIRTNYYGEINETLLNADMAEGFANGVGDSYSYYMTASEYDVYKNEM
                     NGQKMGVGIIPAFNEDDMCLYAAEVSDGSPAALNNMTKGDRIVTIDEEEVTVYNYKEL
                     VKKLEGEKLSSVNVAFIHDGNLVAANMVKGYTAQTVYYETVGEVGYVKITDFYSTTAS
                     QLKNAVNTLIKQGVSGIIIDLRAVSNGTVEYAADALDVLVPVASDGTKALATAVDKNG
                     EAIETFTSDADSINVSTVILVDKDTSGPAELFACDMRDFGKAKLIGTATKGNGTMQRV
                     YQLSDGGAVKLTVAEIKPYTSDVYNGVGLTPDYLVELSSQKKNRLALLSHSEDEQYQK
                     ANSLLTSSAGADEQ"
BASE COUNT          986 a          901 c          834 g          939 t
ORIGIN      
        1 taagatacaa gggtcacgat taccttgtaa accgcttccc cggaatatat gagggatgct
       61 taaaacacgg cgttgatatg accaaggatt taattcccgt atacccgtgc cagcactacc
      121 tcatgggcgg aatcgacgtt gaccttgacg cgcgtacttc tatttcgaga atgtatgctg
      181 taggtgaatg ctcgcacacg ggagttcacg gcaaaaaccg cctcgcaagc aactcgcttt
      241 tggaagcact tgtattcgga aagcgcgcgg cagacgatat agcgtctctt tctaaaaagg
      301 accctgacca cgtgaccgtc acggagcaca aaacggatat ttcgggtgct ccgctgccaa
      361 agggaatgcg tacggaaatt cgctcgatta tgcaacgttc ttactttgta attccggata
      421 tggacgcggt aagagtaggc ttgaagcgcg ttgacactat acttatgaga cttaaaaacg
      481 gcaaatttgc aattacaccc gactattgtg aggctttaag tcttgccacg gtagcccata
      541 taatcttaaa agaggtggac gaaggatgat tttaccgcag ttttacacgg acgacattat
      601 taagcgcgcc atacttgagg atattaacta tgtcgatgtg gcgtcggatt acctgatatc
      661 cgaggagcag cgcaatgagg catatttcgt ctcaaaggca gacggcgttc tctgcggcat
      721 agatgtcgca atgagagttt ttgaactttt ggacgattct tttactgcca aggtatatat
      781 gcacgacggc gacaaggtga aaaagggcga ccttatagcc gaattcagcg gaaaaaccac
      841 gcttctttta aagggcgaaa gaactgcttt gaacttgttg cagcatatgt cgggaatagc
      901 cacggcgacc gcaaaggcag taaaactctg tgagggaacg tcggcttcga tagccgacac
      961 aagaaagacc cttccgtctc tgcgtgccct tcaaaaatac gcggtaacct gcggcggagg
     1021 caaaaaccac cgttttaatc tttccgactg cgctatgctc aaggataatc acatagacgc
     1081 cggaggcggc ataacgggag ccgtaaaaaa gcttcgcgaa aagataggtc atacggtgaa
     1141 aattgaggtt gaaacacgca accttgatga agtaaaagag gcggtcaagg caggagccga
     1201 cataataatg ctcgataata tggactgtga caccatgaaa aaagccgttg agataatcgg
     1261 cggcaaggct cttacggagg cttcgggcgg aattaccgac gaaaccatac ccggcgttgc
     1321 ggcgtgcggc gtagacataa tatcaatcgg agcgctgact cactccgttc aggcatttga
     1381 catttccatg aaaatgaaaa aataaaaagc cacccccaag atgtgcctta gcttatcttg
     1441 agggtatgtt attttgaagc aataatgcct tataaacaaa aagcagcggc gccgaaagca
     1501 aaaagtttcg gtgccgctat tttttgttcg gctacagaaa aaaagtattg ttacactaag
     1561 ctgccgctta ttcaattctg cctttagagc taatgtccgc aaggcggtca aagcacagct
     1621 cggaaagacg tgtcgcaccg cacaattcat acaaggcgga agcaaacaag agcgaattac
     1681 acggtggcag aacctttgtt atgtcatcgg gcagagtaac tccactgccc gacaaacaga
     1741 cggtacttct cgaatactca ttcctgtata aaaggcaccc tccgtccgga ttgcttttat
     1801 ctgttcgcgg agctattaca aatgtgcagc cgcctgcctc attcacgttt tccgtgacgg
     1861 tcatcgcaag accccaatcg tcatacagcc gtcggcgcac gggagaaaat tcttcgggat
     1921 tacccgttat taccttaaga tttgaggcaa gaggaacaag taaagacagc ttatcctttt
     1981 gaaagccgtt aaaatccata acagccacgc tcgttttttc cggcggaaga gagcatttct
     2041 ttatataatc aaccgcggaa ttaaaaagca taacggacgg cagtatacga ggctcgaaat
     2101 tcttaacctt ttcgtcctca gttatggttc ctccgcacac tataactctg cccgcgcacc
     2161 gcccgagcag gctgtatatt ttacccgtat cgacctcttc attttcatca cacacaagcg
     2221 tcagcgtaaa aaacggagca ctgcctaaga cgcttatccg ctcgataacg ggttcgggcg
     2281 gaaaaagcct gtaatgaagc ctcttaaaaa atccgccgcc gattctttct attttaatta
     2341 ctccgaacat aaaagcaccc ccgcataata ttatgcgggg gttttgattt taatgtttgt
     2401 tgtttattgc tcgtccgcgc ctgccgaaga ggttaataga gaatttgcct tttgatactg
     2461 ttcatcctct gaatgagata aaagcgccaa acggttcttt ttctgtgagg acagctccac
     2521 aagataatcc ggagttaagc ccacaccgtt atacacatcg cttgtatacg gctttatctc
     2581 tgccacggtg agctttaccg caccgccgtc gctcaactga taaacccttt gcatggttcc
     2641 gttgcccttt gtcgccgtac ctatgagctt tgctttgccg aaatcgcgca tgtcgcaggc
     2701 gaaaagctct gcaggtccgg aggtgtcttt atcgacaagt atcaccgtgg acacatttat
     2761 gctgtcggcg tccgatgtaa aggtctctat ggcttcaccg tttttatcaa ctgcggtagc
     2821 gagtgccttt gttccgtcgc ttgccacagg aacaagcaca tcaagcgcgt ctgccgcata
     2881 ctctacggtt ccgttcgaga ccgcccgcaa atctattata atgccgctca cgccttgctt
     2941 tataagcgta ttcacggcat ttttgagctg cgatgcggtg gtcgaataaa aatcggttat
     3001 tttaacataa cctacctcgc ctaccgtctc ataataaaca gtctgcgcgg tgtagccctt
     3061 taccatattt gcggcaacga gattaccgtc atgaataaac gcaacattta cggacgagag
     3121 cttttcgccc tcaagctttt ttacaagctc tttatagtta taaaccgtga cttcctcttc
     3181 atctatggta actatacggt cgcccttagt catattgtta agtgccgccg gagaaccgtc
     3241 ggagacctct gccgcataaa ggcacatatc gtcctcatta aacgccggaa ttatgccgac
     3301 gcccattttc tgaccgttca tctcgttttt atatacgtca tactcggacg cggtcatata
     3361 atagctgtaa ctgtcgccta cgccgtttgc aaagccctct gccatatccg cgtttaaaag
     3421 cgtttcgtta atctcgccgt agtaattagt gcggataacg tcgtctatct ccgctaaaag
     3481 cgaataagtc tgcgaacgct tcgggagatc tttgattatt gaattgtaca ttttcattga
     3541 cacagccatg gtacctgcca cagagcctgc catagccaca aatatcaatg cgagcgcaag
     3601 gcccagggat attttcctgt tcaaatgaac gcctctcttt cgggggtttt atcaaagacc
//
LOCUS       DBMDVO010000055         3649 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11192_length_3649_cov_1.102311, whole genome shotgun sequence.
ACCESSION   DBMDVO010000055 DBMDVO010000000
VERSION     DBMDVO010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11192_length_3649_cov_1.102311"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1658)
                     /locus_tag="ACZPE2_01415"
     CDS             complement(<1..>1658)
                     /locus_tag="ACZPE2_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR1318537.1"
                     /translation="TLEARSKGKTTDALKSLMKLAPKSAVILKDGEEKTVPIDTVQKD
                     DIFLVRPGESIPADGVIIEGESAVDESALTGESIPVDKAAGDKVSAATVNRSGFLKCR
                     AIHVGEDTTLSQIIKMVSDAAATKAPIAKIADKVSGIFVPAVIIIAVITTAIWLLLGH
                     GVGYALARGISVLVISCPCALGLATPVAIMVGSGLGAKNGILFKTAASLEETGRIQIV
                     ALDKTGTITKGEPRVTDIIPNGETTENELLKAAASLEKKSEHPLAKAVISYAESKNII
                     CDDVSIFKALPGNGLSGTVNGKNIFAGNLNFIKTKVEISPGIKEIAERLSGDGKTPLF
                     FALDGKLLGIIAVADVIKDDSPKAVKELRNMGIRVVMLTGDNERTANAIGKSAGVDEV
                     IAGVLPSGKEEAIKKLKKLGKVAMVGDGINDAPALTRADIGLAIGAGTDVAIDAADVV
                     LMKSRLSDVPAAIRLSRATLRNIHENLFWAFFYNTIGIPIAAGVFIPLGLTLNPMLGA
                     AAMSLSSFCVVTNALRLNLVNIRDARHDHKTKPAKAVHGPAEKE"
     assembly_gap    1659..1668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1669..2295)
                     /locus_tag="ACZPE2_01420"
     CDS             complement(<1669..2295)
                     /locus_tag="ACZPE2_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1318538.1"
                     /translation="MTQYNVTGMSCAACSARVEKAVSKVPGVTSCSVSLLTNSMGVEG
                     TASDKAIISAVQKAGYGASKKGGSKAAESADESALRDTETPKLKKRLFWSVGFLIILM
                     YFSMGHMMWGWPLPSFYSSNHVAMGLTQLLLTVIIMVINQKFFISGFKSLWHRAPNMD
                     TLVALGSTAAFVYSTYALFMMTDAQTRGDMQAVNTYMMDFYFESAAMIL"
     gene            complement(2295..2666)
                     /locus_tag="ACZPE2_01425"
     CDS             complement(2295..2666)
                     /locus_tag="ACZPE2_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1318539.1"
                     /translation="MNVVLIIILIAVVVLAAVSGAKHFKGQGGCCGGGGELRPEHKKL
                     DGPVTEEKIISIDGMSCENCRNRVERAINKIDGASARVNLRKKEAVVSLDRKIDDETL
                     KNAVESAGYKAVSVKTREVKK"
     gene            complement(2687..2995)
                     /locus_tag="ACZPE2_01430"
     CDS             complement(2687..2995)
                     /locus_tag="ACZPE2_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1318540.1"
                     /translation="MEKEKCCHCSQRKKERSPEEYKKLIHRLNRIEGQIRGIKGMVEN
                     NAYCPDILIQSAAVNAAINAFNKELLANHIRTCVADDIRNGKDESIDELVVTLQKLMK
                     "
     gene            complement(3165..>3649)
                     /locus_tag="ACZPE2_01435"
     CDS             complement(3165..>3649)
                     /locus_tag="ACZPE2_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014926.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HIR1318541.1"
                     /translation="AFTLLRFGTQMGLIPELNYVKWYGRGPQETYIDRKTGGKIAIHE
                     MSVDDLEHHYMRPQENGNRVDVRYVEITDKDGKGLKFTAPYNTPLAFSAWHYTQNELE
                     KATHIHELKHKDITTFNFDLTQLGVGGDMPGDAHVREPYILHPNKKYAYSFTIESIKK
                     "
BASE COUNT          795 a          908 c          835 g         1101 t
ORIGIN      
        1 cttccttttc cgccggccca tggacggcct tggcgggctt tgtcttatga tcatgcctcg
       61 cgtcccgtat gttcacgaga ttaagtctca gggcattggt tacaacgcag aagcttgaaa
      121 ggctcattgc cgcagctccg agcatggggt tcagcgtaag tccgagaggg ataaatacgc
      181 cggcggctat cggaatacct atcgtgttgt agaagaacgc ccaaaacagg ttttcgtgta
      241 tatttctaag cgtcgctctg ctgagcctta tggctgcggg tacatcggaa agacgactct
      301 tcatcagaac gacgtcggct gcatctatgg caacatctgt gcctgcacct atggctaagc
      361 ctatgtcggc tcttgtaagg gcgggtgcgt cgtttattcc gtcgccgacc attgccacct
      421 tgccgagctt tttaagcttt ttgattgcct cttctttacc gctcggtaaa acccctgcga
      481 ttacttcgtc aacaccggct gatttaccta ttgcgtttgc ggtacgctcg ttgtcgcccg
      541 tgagcatcac aacgcgtatt cccatattcc tgagctcttt aacggcttta gggctgtcgt
      601 ctttaataac gtccgccacg gctataatgc cgagaagctt cccatccaga gcgaagaaga
      661 ggggagtttt accatcgccc gacagccttt cggcaatttc cttgataccg ggcgatattt
      721 cgacctttgt ttttatgaaa ttgagattac ctgcgaatat attctttccg tttacggtgc
      781 cgctgaggcc gtttccgggc aaagctttaa agatagatac gtcgtcgcag atgatatttt
      841 tgctctccgc atatgaaatt acagcctttg caagcggatg ctcactcttc ttttcaagag
      901 acgccgcagc tttaagaagc tcattttctg ttgtttcacc gtttgggata atgtccgtaa
      961 ctctcggctc gccctttgtt attgtgccgg tcttgtcgag tgccacaatc tgtattctgc
     1021 ctgtttcctc aagagacgcc gctgttttga aaagaatacc gttctttgcg ccgagaccgc
     1081 tgcccaccat tatcgcaacc ggagtcgcaa gacctaaggc gcacgggcat gatattacga
     1141 gaaccgaaat tccgcgtgca agcgcatagc ctacgccgtg accgagcaga agccatattg
     1201 cggttgtgat taccgcgata attattacag ccgggacgaa tatgcccgaa accttatccg
     1261 cgatttttgc tatcggggcc tttgtggctg cggcgtcgct gaccatttta atgatttgcg
     1321 atagggttgt gtcctcacct acgtgtatgg cacggcattt caaaaatccc gaccgattga
     1381 cggttgccgc cgaaaccttg tctcctgcgg ctttgtctac aggaatgctc tcaccggtaa
     1441 gcgccgattc gtctacggca ctttcaccct cgattatcac accgtctgcc ggtatactct
     1501 cgccggggcg tactaagaat atatcgtctt tttgtaccgt gtcaataggc acggtctttt
     1561 cttcgccgtc ttttaaaatt accgcacttt ttggtgcgag cttcataaga ctctttaatg
     1621 cgtcggtcgt tttgcccttt gagcgcgcct caagggtcnn nnnnnnnnga gaatcatagc
     1681 ggcggactca aaataaaagt ccatcatata ggtgtttaca gcctgcatat cgccgcgtgt
     1741 ctgagcgtct gtcatcataa agagagcgta agtgctgtat acgaatgcgg cggttgaacc
     1801 gagggctacg agggtatcca tattcggggc tctgtgccac agacttttaa aaccgctgat
     1861 aaagaacttt tggtttatta ccataattat aacggtaagc aaaagctgag taagtcccat
     1921 tgcaacgtga ttgctgctat aaaaagaggg gaggggccat ccccacatca tatgacccat
     1981 cgaaaaatac atcagtatta taagaaatcc gacggaccaa aacaaacgct ttttgagctt
     2041 tggtgtttct gtgtcgcgga gtgcggattc atcggcactc tcggcggctt tacttccgcc
     2101 tttcttagat gctccgtagc cggctttttg tactgcggat attatagctt tgtccgatgc
     2161 cgtcccttca acgcccatgg agttggtgag taaactgaca gaacacgacg ttacgccggg
     2221 cacttttgat accgcttttt caactctcgc agaacacgcc gcacagctca taccggtgac
     2281 gttatactgt gtcattattt ttttacctcc cgtgtcttta cggaaacagc tttgtagcct
     2341 gccgattcaa cagcgttttt gagcgtttcg tcgtcaatct ttctgtcgag cgaaacaacc
     2401 gcttcttttt tgcgaagatt tactctcgcc gaagctccgt ctattttgtt tatagctcgc
     2461 tcaacgcgat tccggcagtt ttcgcagctc attccgtcta tcgaaattat cttttcttcc
     2521 gttacggggc cgtcgagctt tttgtgctcg ggtcttaatt ctccgccgcc accgcaacag
     2581 ccgccctgac ctttaaagtg tttcgcgccg gaaaccgccg ccaataccac aactgctatg
     2641 agaattatta ttaaaacaac attcattata ttttaccgtt ctttctttat ttcataagtt
     2701 tttgaagtgt aaccacaagc tcgtcaatcg attcgtcttt gccgttccta atatcgtcgg
     2761 caacgcatgt tctgatgtga tttgcaagca gttctttatt aaaagcattt atcgcggcgt
     2821 ttacggcggc ggattgtatg agtatatccg ggcagtaagc gttgttttcc accatgccct
     2881 ttattccgcg gatttgcccc tcgattcggt taagcctgtg tataagcttt ttatattcct
     2941 cgggggaacg ctcttttttt cgctgtgagc agtgacagca cttttctttt tccatgaacc
     3001 acacctcgtt tctataccct tagggggtat tttgagtata tacccgtgag gggtatattg
     3061 tcaagtctga tttatttttt ttgtttttat atgtgactat gctatggcaa aacaaaaaaa
     3121 taccggagtc tttttgcgac tccggtaaat atcattaaac cgtattattt ctttatgctc
     3181 tctatggtaa aggaataagc atatttcttg ttaggatgga gtatatacgg ctcacgcacg
     3241 tgcgcgtcac cgggcatatc tccgcctacg ccgagctgag ttaaatcaaa gttaaacgtc
     3301 gtaatatcct tgtgctttaa ctcatgaata tgcgtggctt tctcaagctc attctgcgtg
     3361 taatgccagg cgctgaatgc gagcggagtg ttgtacggag cggtaaattt aaggcccttg
     3421 ccgtccttgt cggttatttc gacataacgg acgtcaactc tgttgccgtt ttcctgcgga
     3481 cgcatatagt ggtgctcaag gtcgtcaacg ctcatctcgt gaatagctat tttgccgccg
     3541 gttttacggt ctatatatgt ttcctgtgga cctctgccgt accactttac gtagttgagt
     3601 tcgggtataa gccccatctg cgtaccgaat ctcagaagtg taaaggcct
//
LOCUS       DBMDVO010000056         3626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11297_length_3626_cov_1.236596, whole genome shotgun sequence.
ACCESSION   DBMDVO010000056 DBMDVO010000000
VERSION     DBMDVO010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_3626_cov_1.236596"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            119..379
                     /locus_tag="ACZPE2_01440"
     CDS             119..379
                     /locus_tag="ACZPE2_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318542.1"
                     /translation="MIKGVNRQVLEIKDTGCEYFTQALFFVDPKFSTLPEKTLREKAE
                     ASLKDPPGVPVSKKQKALKIILKITNIAAAAGAGAAICAFIK"
     gene            complement(372..2117)
                     /locus_tag="ACZPE2_01445"
     CDS             complement(372..2117)
                     /locus_tag="ACZPE2_01445"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1318543.1"
                     /translation="MLRRDDGDFCEHKMCWAGMNGDDKEIWDIELSVPECGLYWYHFD
                     YDSSWGRGSVYNIGDGKGDVWNGRNSRLQWQLTVYDKNYKTPSWLKGGIMYQIFPDRF
                     FRSGEKKENVPSDRILRDDFDGEPNWRPNEKGEVLNNDYFGGDLRGITEKLSYLFELG
                     VTCIYLNPIFEAHSNHRYNTADYMKTDPLLGTEEDFKKLCESAGRVGIKIILDGVFSH
                     TGDDSRYFNRYSRYDTVGAYNSKSSPYFKWYKFDRYPDEYKSWWGFKTLPEVTEECPD
                     YDEFICGKNGVIRKWLRLGASGWRLDVADELPDCFIDDLRVAVKTEKPDGILLGEVRE
                     DATTKFSYGTRRRYLLGVQLDSVMNYPFAEAVTDFARNGVAESFESSVMTIIENYPKE
                     ALDVLMNHIGTHDTERAITKIAGEKSDYRDRQWQSEHSLSEEEYKRGVKLLKLCAAIQ
                     YTLPGVPCVYYGDEAGMQGYKDPFNRAFFPWGRENRELQNYYIRLGKVRRSTDCLKEG
                     EFIPVSSVLSCVCFERRGKNDSCLLIANRNEQDITYYLPPRWQNTSEVLLGESVGSSV
                     LVPGLSAVILKKETT"
     gene            complement(2250..2822)
                     /locus_tag="ACZPE2_01450"
     CDS             complement(2250..2822)
                     /locus_tag="ACZPE2_01450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1318544.1"
                     /translation="MYTSFEELKKDCLKCNKCPLAATRTNVVFGVGPEDAEVLFIGEG
                     PGENEDLTGQPFVGRGGKLLDQYLYDVDLDRNKNIYIANIVKCRPPKNRDPQPLEQES
                     CEMWLREQVRLMRPKIIVCLGRIAAQKIISPDFKVTKQHGEFFERKGTLLMGTFHPAA
                     LLRNPNNKPAALSDFLALRDKINEICERTY"
     gene            complement(2859..>3626)
                     /gene="prfA"
                     /locus_tag="ACZPE2_01455"
     CDS             complement(2859..>3626)
                     /gene="prfA"
                     /locus_tag="ACZPE2_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1318545.1"
                     /translation="PKDPNDYKNVIVEIRGGAGGEEAALFAGSLFRMYSMYAERKGWK
                     TEIMNENPTELGGYKEISFMIEGEGAYSRLKFESGVHRVQRVPETESQGRVHTSTVTV
                     AVLPEAEEVEFTLDPADLQIDVFRSSGAGGQKVNKTSSAIRVTYLPTGMVVECQDERS
                     QYKNKDKALKVLRSRLLEEKTAKQNAEIAGERRSQVGTGDRSERIRTYNFPQGRVSDH
                     RIGLTLYKIEAIMNGDLDEIIDALVTADTAEKLKAED"
BASE COUNT          849 a          990 c          812 g          975 t
ORIGIN      
        1 ttaaatttgt gtaaaatccc atattgacaa aaatctcccc gattttttct ccgccttttc
       61 cgcataaaaa tcgctcttta aaacaataat aaatacataa attaccggag gaaaaattat
      121 gataaaaggc gtaaacagac aggtgcttga aataaaagat acgggctgcg aatattttac
      181 tcaggcactt tttttcgttg acccgaaatt cagcacatta cccgaaaaga ccctgcgcga
      241 aaaagccgag gcgtccctca aggacccgcc gggagtgccc gtaagcaaaa agcaaaaagc
      301 gctcaaaata atccttaaaa taacaaatat cgcggcagcg gcaggcgccg gcgccgcaat
      361 atgcgctttt atcaagtagt ttcttttttc agtatgaccg ccgatagccc cggcacaaga
      421 accgagctgc cgacactctc gcccaaaagc acttcgcttg tgttttgcca gcgcggcgga
      481 agataatatg ttatatcctg ctcatttctg ttggctatca gcaggcacga gtcattttta
      541 ccgcgacgct cgaagcagac gcaggagagc accgaggaaa caggtataaa ctcgccttcc
      601 tttaagcagt cggtgcttct tctcaccttg ccgagcctta tatagtaatt ctgaagctcg
      661 cggttttctc tgccccacgg gaagaaagcg cggttaaacg ggtccttgta accctgcatg
      721 ccggcttcgt cgccgtagta gacgcacggc actccgggga gggtatactg aatcgccgcg
      781 cagagcttta atagctttac tccgcgctta tattcctcct cggacaggga atgctccgac
      841 tgccactgcc tgtcgcgata atcgctcttt tcgccggcta tctttgttat cgctctctcc
      901 gtatcgtgag tacctatgtg gttcatcaaa acgtcgagtg cttccttagg atagttttca
      961 ataatcgtca taaccgacga ttcaaagctc tctgcaacgc cgtttctcgc aaagtccgta
     1021 acggcttccg caaacggata attcataacg ctgtcgagct gaacccccaa aaggtatctg
     1081 cgccgtgtgc cgtatgaaaa ctttgtggtg gcgtcctccc taacctcgcc gagcaatatg
     1141 ccgtcgggtt tttccgtctt taccgccacg cgcaaatcgt ctataaagca gtcgggcagc
     1201 tcgtcggcaa cgtcaagccg ccagccggac gcgccgagcc gcagccattt ccgtataact
     1261 ccgtttttgc cgcagataaa ttcgtcgtaa tcggggcact cctcggtaac ctccgggagc
     1321 gttttaaagc cccaccagct cttatattcg tcggggtagc ggtcaaattt ataccacttg
     1381 aaatacggcg acgattttga attatacgca cctacggtat catatcgcga atagcggtta
     1441 aaataacggc tgtcatcgcc ggtatgtgaa aaaacaccgt cgagaattat ttttattccc
     1501 actctgccgg cactttcgca tagcttttta aaatcctcct ccgtgccgag aagcgggtct
     1561 gttttcatat aatccgccgt gttgtagcgg tgattcgaat gtgcctcaaa tatcgggttt
     1621 aaatatatgc aggtgactcc gagctcaaaa agatatgaga gcttttcggt aattccgcga
     1681 aggtctccgc cgaaatagtc gttattgagc acctcaccct tttcgttcgg tcgccaattc
     1741 ggttcgccgt cgaaatcgtc gcgcaaaatt ctgtcggacg gtacgttttc tttcttctcg
     1801 cccgaacgaa aaaatcggtc gggaaatatc tgatacataa ttccgccctt gagccatgac
     1861 ggcgttttgt agtttttatc gtatacggta agctgccatt gaagacggct gttcctgccg
     1921 ttccaaacat cgcccttgcc atcaccgatg ttatacacgg agcctctgcc ccaggacgaa
     1981 tcatagtcaa aatgatacca gtaaagtccg cattcgggaa ccgacagctc tatatcccat
     2041 atctccttgt cgtcaccgtt catgcctgcc cagcacatct tatgctcgca aaaatcaccg
     2101 tcgtcgcgcc gcagcataaa ataagccgcc gtggcggaaa aagagcgcgg caaaataagc
     2161 ctgagacgaa atttttctcc ggtttttacc gcaccgaatt tacttttgta aaacgtgtct
     2221 cgcgagttaa agggtataaa atccatatat caatacgttc tttcgcaaat ttcgttgatc
     2281 ttgtcccgaa gtgccaaaaa gtccgagagt gctgccggct tgttgttcgg gttgcgcaaa
     2341 agcgccgccg gatgaaaagt tcccatcaaa agggtccctt tgcgctcaaa aaattcgccg
     2401 tgctgctttg tcactttaaa atcgggggaa attatcttct gtgccgctat tctgccgagg
     2461 caaactatga ttttcggtct cataagccta acctgctccc ttagccacat ctcgcacgat
     2521 tcctgctcca gcggctgcgg atcgcggttt ttcggcggac ggcattttac gatatttgct
     2581 atgtagatat ttttatttct gtccaaatcc acatcataca gatattggtc gagcagcttt
     2641 ccgcctctgc ccacaaacgg ctggcccgta aggtcctcgt tttcgccggg tccctctccg
     2701 atgaaaagaa cctcggcgtc ctcgggtccc acgccgaaaa caacattcgt tctcgtcgcg
     2761 gcaagcggac atttattaca ctttaaacag tcttttttaa gttcttcaaa tgaggtatac
     2821 ataaattatt gcctttcttt ttactgtatc gcccgagatt aatcctctgc tttcagcttt
     2881 tcggctgtgt ccgcagtaac aagtgcgtct atgatttcgt caaggtcgcc gttcattatc
     2941 gcctctatct tgtaaagcgt cagccctatt ctgtggtcgg acacacgccc ctgagggaaa
     3001 ttgtacgtgc gtattcgctc gctcctgtcg ccggtgccga cctgtgaacg cctctcacct
     3061 gcgatttcgg cattttgctt tgcggtcttt tcctcaagga gccttgaacg caaaaccttg
     3121 agggccttat ccttgttttt atactgactg cgctcatcct ggcactcaac gaccataccc
     3181 gtgggcagat acgtaacgcg aattgcggac gaagtcttgt ttaccttttg cccgcccgca
     3241 ccgcttgagc ggaaaacatc tatctgaagg tcggcggggt caagcgtaaa ttcgacctcc
     3301 tccgcctcgg gaagtaccgc tacggttacg gtggacgtgt gaactctgcc ctggctctcg
     3361 gtttcgggca cgcgctgaac gcggtggacg ccgctctcga atttgagccg cgaataagcg
     3421 ccctcgccct ctatcataaa gcttatttcc ttatatccgc cgagctcggt cggattttca
     3481 ttcattattt cggttttcca gcccttgcgc tcggcataca tactgtacat acggaaaagc
     3541 gagccggcaa aaagagctgc ctcctcgccg cctgcaccgc cgcgaatttc gactattacg
     3601 tttttatagt cgttcgggtc tttagg
//
LOCUS       DBMDVO010000057         3602 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11432_length_3602_cov_0.999143, whole genome shotgun sequence.
ACCESSION   DBMDVO010000057 DBMDVO010000000
VERSION     DBMDVO010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11432_length_3602_cov_0.999143"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="ACZPE2_01460"
     CDS             <1..835
                     /locus_tag="ACZPE2_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017328.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HIR1318546.1"
                     /translation="ALDYFVRNSDNRATVYAAVAEDTAGQILSAEMGQRVIPAKELSN
                     VLSSSKYNSVTAERAFYQFVNGVTGKTSSAFLPIIAIEQNEKDDDGGEDGRKDSARPV
                     VRGAALFNGDKMENVLPQDNVPALLMLCNDFNNGSLTAVLDDGTRVGVLLTKSKTKIK
                     TSVKNGCPSFDISVDCVADCTETSKIMNEAFTPEVAENIKTALEKAVRRNIDDTVKKC
                     FTEFNKDPFGFDKRVKRADAEYYRAESDNWKSVLQKAGYSVTVNVKLRRAGDENMVPS
                     S"
     gene            868..1170
                     /locus_tag="ACZPE2_01465"
     CDS             868..1170
                     /locus_tag="ACZPE2_01465"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR1318547.1"
                     /translation="MNTFDKIYEQVKKIPYGRVATYGQIALLAGNPRWSRVVGYALHV
                     NPDPENVRCYRVVNRFGEVSKAFAFGGENEQIRLLKSEGVEVDENGRVDLKKYGMV"
     gene            complement(1218..2075)
                     /locus_tag="ACZPE2_01470"
     CDS             complement(1218..2075)
                     /locus_tag="ACZPE2_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1318548.1"
                     /translation="MNFGEFQEKKSRGTFDFPIELHHVTKEHPRYQMPFHWHMDYELI
                     RVLKGSLVLSLNEETLNLTENSGIVLVCDGVLHGGMPVDCVYECLDFDLNEFLRGCSA
                     GKKDIDDVQSHRIILDNFFAVDSEEYKTANRLFDTFIEEKKGYEFIIQGLLFELMGQM
                     LGNHRYKISDDETYKSLKRIRQLKSSLKIIREEYMNQLTLGNLAEAADMSPKYFCRFF
                     SEMTGKTPIEYLNYYRIECAAEQLLYTDDLVTDIALNCGYNDLSYFVKTFKKYKSQTP
                     KQFRKNKHT"
     gene            2204..3149
                     /gene="fucI"
                     /locus_tag="ACZPE2_01475"
                     /pseudo
     CDS             2204..3149
                     /gene="fucI"
                     /locus_tag="ACZPE2_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459161.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; frameshifted;
                     incomplete; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     assembly_gap    3150..3159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3160..>3602
                     /gene="fucI"
                     /locus_tag="ACZPE2_01480"
     CDS             <3160..>3602
                     /gene="fucI"
                     /locus_tag="ACZPE2_01480"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459161.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HIR1318549.1"
                     /translation="NCDFPEALLNTSFDWNGARETYVLATENDTLNGVSMLFGKLLTN
                     TAQLFSDVRTYWSPEAVKKATGYELEGVAKESKGFLHLINSGASALDFCGEVKDENGN
                     GIVKPFWEMTDKDIKACTDATTWNAADLGYFRGGGFSSRFLTRSE"
BASE COUNT         1038 a          750 c          891 g          913 t
ORIGIN      
        1 agctcttgat tatttcgtgc gaaattcaga taaccgtgct accgtctatg cggctgttgc
       61 cgaagatacc gcgggacaga tcttgagtgc cgaaatgggg cagcgcgtaa ttcccgcaaa
      121 ggagctgtct aatgtacttt cctcgtcaaa gtataattcc gtaaccgccg agagagcttt
      181 ttatcagttc gtaaacggcg ttaccggcaa gacctcatcg gcatttcttc cgataatcgc
      241 gatagaacaa aatgaaaaag acgacgatgg tggcgaggac ggccgaaagg attctgccag
      301 gcccgtggtc aggggagccg cgctgtttaa cggcgataag atggaaaacg ttctgccgca
      361 ggacaatgtt ccggcacttt tgatgctgtg taatgacttt aacaacggct ctcttacggc
      421 tgtgcttgac gacgggacac gcgtcggcgt tttgctgact aagtcaaaaa ccaagataaa
      481 aacatctgtg aaaaacggat gcccgagctt tgatatttct gtcgattgcg ttgcagattg
      541 taccgagacg tcaaaaatta tgaatgaagc attcactccg gaggtggcgg aaaatataaa
      601 aacggcctta gaaaaagccg tccgaagaaa tattgacgat accgtaaaaa agtgctttac
      661 cgaatttaat aaggacccgt tcggatttga caagagggta aaaagagccg acgccgaata
      721 ttatagggct gaatcggata actggaaaag cgttttgcaa aaggccgggt acagcgtaac
      781 cgtaaatgtg aagcttcgcc gcgccggaga cgaaaatatg gttccgagca gttgacataa
      841 gggggataac aacggtaaaa ttaaaatatg aatacttttg ataaaatata tgagcaggta
      901 aagaaaatac cgtacggcag ggtcgcaacc tacggtcaga ttgcgcttct cgccggtaat
      961 ccgcgatggt cgcgcgtagt cggatacgct cttcacgtaa atcccgaccc cgaaaatgtg
     1021 cgctgttacc gcgtggtaaa caggttcggc gaggtgtcaa aggcgtttgc gttcggcggt
     1081 gaaaacgagc agatacggct tcttaaaagc gagggcgtcg aggtagacga aaacggcaga
     1141 gtcgatttaa aaaaatacgg tatggtatga aattaaaaaa tccgcaggca cttttttcgg
     1201 tgcgagcgga tttttcttta tgtatgtttg ttttttctga attgttttgg ggtttgagat
     1261 ttgtacttct tgaatgtttt gacaaaatag ctcaggtcgt tgtatccgca gtttagagca
     1321 atatccgtaa cgaggtcgtc cgtgtacaaa agctgctccg ccgcacattc tattctgtaa
     1381 taattgaggt attcgatagg cgttttgccg gtcatttcgg aaaagaaacg gcaaaaatat
     1441 ttgggtgaca tatcggcggc ttctgcgaga ttaccgagag taagctgatt catatattcc
     1501 tctctgataa tttttaagga gctttttaat tgccgtatcc ttttgagact tttatacgtt
     1561 tcgtcgtcgc tgatcttata acggtgattg ccgagcattt gccccataag ctcaaacaaa
     1621 agtccctgta ttataaattc ataacccttt ttctcttcta taaaggtgtc aaacagcctg
     1681 tttgcggttt tatattcttc gctgtcaacg gcaaaaaaat tgtcgagaat tatgcggtga
     1741 ctctgcacat cgtcaatatc ctttttgcct gccgagcagc cgcgcaggaa ttcgtttaaa
     1801 tcgaagtcga gacattcgta aacgcagtca acgggcatgc cgccgtgcaa tactccgtcg
     1861 cacacaagca caataccgct gttctcggta agatttaaag tttcttcgtt gagcgacagt
     1921 acaagactgc cctttaaaac tctgataagc tcgtaatcca tgtgccaatg aaacggcatt
     1981 tgataacgcg ggtgctcctt tgtaacgtgg tgcagctcta tcggaaagtc gaaagtgcct
     2041 cgtgattttt tctcctgaaa ttcaccgaaa ttcataattt acctcaaaag tgaaaattgt
     2101 tatatttttt atttattata ctacaagaat ttatataaag acaacagtat aattaaaaat
     2161 atatgaaaaa aatatattta atttttataa acaggaggaa attatgagta aaacaagact
     2221 tataggcgat tatccggtta taggaatccg cccgacaatc gacggcagac gcggcccttt
     2281 aaaggtgcgt gagtcacttg aagagcagac catgaatatg gctctttcgg cggcaaagct
     2341 ttttaccgac aatcttcgct atacaaacgg cgagccggta aaggttgtaa ttgccgacac
     2401 aaccataggc agagttgccg aggcggcggc atgtcaggat aagtttaaaa aagcgggcgt
     2461 agacataact cttacagtta ctccgtgctg gtgctacggc agcgagacta tggatatgga
     2521 cccgaatacc ataaaaggcg tttggggctt taacggaacg gaaagaccgg gtgcggttta
     2581 tttagcttcg gtgctcgcct cacacgctca aaagggactc cctgcgttcg gaatctacgg
     2641 cagagacgta caggaggctg acgccaccga aattcccgac gatgtaaaag aaaagttact
     2701 gcgtttcggc agagcggcag ttgccgcggc taccatgcgc ggaaaatcat atcttcagat
     2761 aggctccata acaatgggta tagccggctc tataataaat cccgatttct ttgaagagta
     2821 tctcggtatg cgcgttgagt cggtagacga ggtggaaata atccgccgaa tgaccgaggg
     2881 catatatgac gaggcagagt ttaaaaaggc tctcaaatgg acaaaagaaa actgcaagga
     2941 gggcttcgat aagaaccccg actggtttaa aaagaccgat aaggaaaaag aagaggcttg
     3001 ggaatttgtc gttaagatga tgtgcataat aaaagacctc tataacggca acgaaaatct
     3061 ccccgacggt gccgaggagg aaaaagtagg tcacaacgca atctgcggcg gcttccaggg
     3121 tcagagacag tggacggatt tttttttttn nnnnnnnnna actgtgattt ccccgaggcg
     3181 ctcttaaata cctcgttcga ttggaacggt gctcgcgaaa cctatgttct cgctaccgaa
     3241 aacgatacgt tgaacggcgt ttccatgctt ttcggtaaac tgcttacaaa taccgcacag
     3301 ctcttctccg atgttcgtac atattggagc cccgaggcag tcaaaaaggc tacgggctat
     3361 gagcttgagg gcgtggcaaa ggagtcaaag ggatttttgc atctgataaa ttcgggcgcg
     3421 tccgctctcg atttctgcgg cgaagtgaaa gacgaaaacg gcaacggtat tgtaaaaccg
     3481 ttctgggaaa tgaccgataa ggatatcaaa gcctgcaccg acgccacaac atggaatgcg
     3541 gcggatctcg gatatttccg cggaggcgga ttctcttcaa gatttttaac aagaagcgaa
     3601 at
//
LOCUS       DBMDVO010000058         3598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11449_length_3598_cov_1.134973, whole genome shotgun sequence.
ACCESSION   DBMDVO010000058 DBMDVO010000000
VERSION     DBMDVO010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11449_length_3598_cov_1.134973"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="ACZPE2_01485"
     CDS             <1..1828
                     /locus_tag="ACZPE2_01485"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR1318550.1"
                     /translation="SAKENKEYTSDNFLSVADGIIAWKKADNGSEPDGYLINDKYLEN
                     AGTTPGDWYPIALGRLGIQDNNEGYLAVIKDRVEERYRESGRLSAAKATEWHRIALAV
                     LAMGGDPTNFGTDENGAPINLIADGTYDRGKTTPLGRQGINGWIWGLITLDSMRYAVP
                     DGAYNTRDDIIVEIISKQLSDGGFALSSDDADPDITAMAITALAPYYNSEKIYSYHRK
                     ALSADESKTVHEVVDEAVLCLSRLQLDTGDYKSWGTQNVESTDWVAVALCSLNIDPLS
                     DSRFIKNGNTLFDGIMRYRMTDGGFVHSFTYDPDNPTSLPDKSNTMASEQTLYTMASL
                     WRQKNGMRTLFDFRKEQSSALKSRINDLTSDISKIGNKTDKQTLRELLAVFYSLPETE
                     RCYVSNYWTLSDEAKKRGIDVSEIAGETAVIVFSDDDANDDAILYFTDSDRAMVDSLP
                     EKLTTEQYVLVVTLLDKLERSEDFDGKDAYLRKLVSAKEQIAAVQAEIDSLNDDIKAE
                     LYPFDKITLKDRGKVNKIVKRYNALSEYDRAKIERWEDVVKTKTKLDNIVRAIVISVV
                     LFVLAVGLTVFIIIRIRRRKMKKTLEMEELAAMYKDEDDEMR"
     gene            1856..2410
                     /locus_tag="ACZPE2_01490"
     CDS             1856..2410
                     /locus_tag="ACZPE2_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIR1318551.1"
                     /translation="MKKDVLALLSLALIIAVLIHGTNIQSVDEYYLTHIDDITPQSET
                     VFISIRCDTVLQNYDELDKALQSDKYVPQNGVILPETEYVLRPGDTVFDILDRAVRYN
                     KIQMEYQGADKNSYGSVYVQGINYLYEFSCGPLSGWMYQVNGEFPNYGCSKYKLKDGD
                     RIAWVYTCDLGKDVGCIWMGDETG"
     gene            2407..3288
                     /locus_tag="ACZPE2_01495"
     CDS             2407..3288
                     /locus_tag="ACZPE2_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIR1318552.1"
                     /translation="MKAFGGYHPAVTFTYFISVLLTAMFVWNPVIQLTALIGGIMFSL
                     MLVRKKAILSDMGFYLPLFLLVAVTNPLFSHNGVTPLFFMNGNPVTLEAVVYGVFISV
                     MVVGVLFWCKCYNEIMTEDKFLYLFGRIIPKMSLVLSMALRFVPMFKRQMKNVSRAQK
                     AMGLYSQKSYVDRLRGGIRVFTAMISWSLENSMELSSSMRARGYGLHGRTNFSLFKFG
                     ARDAAVLLSGLLLFAAVLIGVGSGSIDFTFYPELGKISLSLKSLITYISFFILSFLPF
                     FIEVKENLKWKYYISKI"
     gene            3261..>3598
                     /locus_tag="ACZPE2_01500"
     CDS             3261..>3598
                     /locus_tag="ACZPE2_01500"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318553.1"
                     /translation="MEILHIENLNFRYPKAEKDTLHNISLNIGQGDFTVICGATGCGK
                     TTLLKLIKNELAPAGEKSGEILYNGKSIDSLSLRESACAIGFVGQNPDNQIVTDKVWH
                     ELSFGLENLGV"
BASE COUNT         1000 a          752 c          902 g          944 t
ORIGIN      
        1 atcggcaaaa gagaataagg agtatacgtc cgataatttc cttagcgtcg cggacggtat
       61 tatcgcatgg aaaaaagccg ataacggctc ggaacccgac ggatatttga ttaacgataa
      121 atatttggaa aatgcgggta ctactccggg ggattggtat cccatagctc tcggcagact
      181 cggaatacag gataacaacg agggctacct tgcggtcata aaagacaggg tagaggagcg
      241 ttatcgcgaa agcggaaggc tcagtgccgc aaaggcaacc gagtggcaca gaatagcact
      301 cgccgttctc gctatgggcg gcgacccgac aaatttcgga accgacgaaa acggcgcacc
      361 gataaatctg atagcggacg gaacctacga ccgcggcaag acaacgccgc tcggaagaca
      421 gggcataaac ggctggatat gggggcttat aactcttgac agtatgcgct atgccgtgcc
      481 tgacggagcg tacaacaccc gcgacgatat aatcgttgaa ataatttcaa agcagctttc
      541 cgacggcgga tttgccctga gctcggatga tgcagacccc gatattaccg ctatggcgat
      601 aacggcactc gcaccatatt ataattccga aaaaatatat tcttatcaca gaaaagcgtt
      661 gtccgccgat gagagcaaaa cggtgcacga ggttgttgat gaagcggttt tgtgcctttc
      721 acgtctgcaa ctcgacacgg gcgactacaa aagttgggga actcagaatg tagagagtac
      781 cgattgggtg gcggtggcgc tgtgcagtct taatatcgac cctctctcgg acagccgttt
      841 cataaaaaac ggcaacaccc tttttgacgg tataatgcgt tacagaatga cggacggcgg
      901 atttgtccat tcgtttactt acgaccccga caatcccaca tctttgcccg ataaatcaaa
      961 taccatggcg tcggagcaga ccctttatac catggcatct ctttggcggc aaaaaaacgg
     1021 tatgagaact cttttcgatt tcagaaaaga gcagagctcc gcgcttaaat cgcgaataaa
     1081 cgaccttact tccgacattt caaaaatcgg caataagacc gataaacaaa cgcttcgcga
     1141 gcttcttgcg gttttttatt cgttgcccga aacagagcgg tgctacgtga gcaattattg
     1201 gactttgtcc gatgaagcca aaaaacgcgg aatagacgtt tcggaaatag cgggtgaaac
     1261 cgcggtgatt gtgttttcgg atgatgacgc aaatgacgac gccattttgt attttaccga
     1321 ttccgaccgt gcaatggttg actctctgcc cgaaaaactc actacggagc agtatgtttt
     1381 ggttgtaaca ttgcttgata agctcgaaag gagtgaagat tttgacggta aagacgcata
     1441 tctccgaaag cttgtttcgg caaaggagca aattgccgcg gttcaggcgg aaatagattc
     1501 gctgaacgac gacattaaag ccgagcttta tccgtttgat aaaatcacgc tcaaggatag
     1561 gggcaaggtg aataaaatcg ttaaacgcta taacgcgctc agcgaatatg acagagcaaa
     1621 aatcgagcgt tgggaggatg tcgtcaagac aaagacaaag cttgacaata tcgttcgcgc
     1681 catagtaata tctgttgtat tgtttgtttt agcggtagga cttaccgtgt ttataattat
     1741 aagaatcaga cgccgtaaga tgaaaaaaac tctcgaaatg gaggagcttg cggcaatgta
     1801 taaggatgaa gacgacgaaa tgcggtaatt ttttctcgcg gaaggggagt cataattgaa
     1861 aaaagatgtt ttggcgctcc tgtcgttggc gctgataata gccgtattga ttcacggcac
     1921 aaacattcag tccgttgacg agtactacct tactcatatc gacgatataa ccccgcagag
     1981 cgagacggtt tttatcagta ttcgctgtga taccgtgctt caaaattacg acgagcttga
     2041 caaagccctg caatccgata aatacgtgcc gcaaaacggc gttatattgc cggaaaccga
     2101 atatgtgtta agacccggcg acaccgtttt tgatatactt gacagggcgg taaggtataa
     2161 taaaatacag atggagtatc agggtgccga taaaaacagc tatggcagcg tatacgttca
     2221 gggtataaat tatctgtacg aattttcctg cggaccgctt tcgggttgga tgtatcaggt
     2281 gaacggcgaa tttccgaatt acggatgctc aaaatacaaa ctgaaggacg gcgaccgaat
     2341 agcatgggtc tatacctgcg atctcggcaa ggacgtcggc tgtatatgga tgggagatga
     2401 aacgggatga aagctttcgg cggatatcat ccggctgtta cttttacata ttttatatct
     2461 gtgctgttaa cggctatgtt tgtatggaat cccgtaatac agctcacagc actcatcggc
     2521 ggcattatgt tttcgcttat gctcgtgagg aaaaaggcta ttttaagcga tatgggcttt
     2581 tatcttccgc tttttctgct tgtggctgtt acaaatcctc tgttttcgca taacggcgtt
     2641 actccgcttt tctttatgaa cggtaaccct gtaacgcttg aagcggttgt ttacggcgtt
     2701 tttatctcgg tgatggttgt cggagtgctg ttttggtgca agtgctataa tgaaattatg
     2761 accgaggata aattcttgta tctgttcggc agaattattc cgaaaatgtc gcttgtgctt
     2821 tccatggctc tcagatttgt tccgatgttt aaaagacaga tgaaaaatgt gagcagagcg
     2881 caaaaggcaa tggggctata ttcgcaaaaa agctatgttg acagattacg cggcggaata
     2941 cgtgttttta cggcgatgat ttcgtggtcg cttgaaaatt ccatggagct gtcgtcgtct
     3001 atgagagcgc gcggatacgg tctgcacggc agaacaaact tttcgctgtt taaattcggg
     3061 gcgagagacg cggcggtttt attgtcggga ttattgcttt ttgctgccgt acttataggc
     3121 gtcggcagcg gaagcatcga ttttacattt tatccggagc tcggcaaaat cagcctttcg
     3181 ttaaaatccc tgattactta catttctttt tttatattat cgtttcttcc gttttttatt
     3241 gaggtaaagg agaatttaaa gtggaaatat tacatatcga aaatttgaat ttccgttacc
     3301 ccaaggcgga aaaagacact cttcacaata tttctcttaa catcgggcag ggtgatttta
     3361 ctgtaatatg cggcgcaacg ggctgcggaa agaccaccct tttaaagctg attaaaaacg
     3421 agcttgctcc cgcaggtgaa aagagcggcg agatactcta taacggtaaa agcatcgatt
     3481 ccctcagcct tcgcgagagt gcatgtgcca tagggtttgt cgggcaaaat cccgataatc
     3541 agatagttac ggacaaggtt tggcacgagc tgtcgttcgg ccttgaaaat ctcggtgt
//
LOCUS       DBMDVO010000059         3543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11731_length_3543_cov_1.873620, whole genome shotgun sequence.
ACCESSION   DBMDVO010000059 DBMDVO010000000
VERSION     DBMDVO010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_3543_cov_1.873620"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3479)
                     /locus_tag="ACZPE2_01505"
     CDS             complement(<1..3479)
                     /locus_tag="ACZPE2_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1318554.1"
                     /translation="MYKKLILKRSLCALLSVIMIAVCLPLSVFAADPSEPQFSSDAAN
                     TLMKNTKPTGFDNTTNPYGYDVGRPFAMVEKNELLYFETYGTSATGKIADVGTPESLK
                     NFISKKGTASDASLPNMDISALQTYYAFVQSVSFDPFGSGRRDHVAYVGLDKSDKQVY
                     LHIYDTRTGEYKGKQWVGEMNWMFDNKGLIRALNLETNAYLNITAGDFDGDGKDTLII
                     YVPHTKRSTGLMQTFDYEPFVGCFLYEYSFTGKNSFVLLNNTSIMGEGKKLGFGNDML
                     HDSYKKLYHTTVGSPTDPGANGDSMYGSSGQTFDNRFHKLGVDMVVGDFNGDQIDDIA
                     VLSYFFINNVNSRNLNLYRPQVKIKYGKAGGSSSGGGSSFVDGDADEKFYVCPEGELQ
                     NFSQNDVVFNSNSMNACSITAGDFNNDGYEDILVAGLRASVAGITLSVASTSSSEIAY
                     TTLLNNGNRGFVQSEYKVMASNGWTKAGSRTKEVVSPLAVEAVAFNGASAADFVFING
                     TLYNYNAQTGELYKLTSNEYSFRTNSNGKTNNFWFEGEIGGKTEFFRSVAVGNFDGNN
                     AGREQIVFVTSAETGNEDKYSFSKGMISATYPDTSDSYEAANGFDLTLSYWSPNEAYG
                     DYYGCTAQVLLTACDRNQDGVIAKYKGVSYAYSDPEVKAVIQAAPYFSALSDSGENET
                     AYTITESFELEQSSSNNVSFSVGMSYSYGGLLAPVEVNISAGYSLEWSKTYSTALQKE
                     YSLTVKARAYNTVLVSRTPVFIYAYEILDENGKWSGDTAITLSIPQQPVYQQLSVDDY
                     NGFVDEYNGYMNNKNYHAVIRESDKTDKDKKYYYPLVKIDKAANWLDGNEGNPFMYNQ
                     LGWGVYSHLGAEQLSKLAIRLGHNGGLNEVSWGKSNIKSVSEEMSHGFYFNFSISFGV
                     FGDGILFSHDVGFETSLSYSNGSGVSTTTTSSEGCTGVVNDIDGPDLASNGIPQNVYT
                     QYTFDWTLGQWFRHLYGDENNKTIFIGYNLTNLSAPSPAVVDLEAELLDEDEIRLTWS
                     QPDKTPGWPDAEGYYLYQVENGEYNRISNLIPVGTTSYDISGLKTNTEYEYVLTTVCK
                     VDGKDKESAWSNSATITTAKRDYKVNYKIDNEKAADISVRHLGNVNIKSGDEINENDI
                     IKIRVSPKNRHFEL"
BASE COUNT          859 a          832 c          843 g         1009 t
ORIGIN      
        1 agttcaaagt gccggttctt tggcgaaact ctgattttaa taatatcgtt ttcattgatc
       61 tcatcgcccg atttaatatt aacattgccg aggtgccgca cactgatatc ggcggctttc
      121 tcgttgtcga ttttatagtt gaccttatag tctctctttg ctgttgttat ggttgcagag
      181 tttgaccagg cactttcctt atcctttccg tctaccttgc aaacggttgt aagcacatat
      241 tcgtactctg tattggtttt aagtccggaa atgtcataag aggtcgtgcc gacaggaata
      301 agatttgaaa tcctgttata ttcaccgttt tcaacttgat aaagatagta gccctccgca
      361 tccggccagc cgggagtttt atcgggctgc gaccaagtaa ggcgtatctc gtcttcgtca
      421 agcagttctg cctcaaggtc gaccactgcc ggtgaaggag cactgagatt tgtcaagtta
      481 tagcctataa agatggtctt gttgttttca tcgccgtaaa ggtgtcggaa ccactgaccg
      541 agcgtccagt caaaggtata ttgcgtgtat acgttttgag gaattccgtt tgaggcaaga
      601 tccggaccgt cgatatcatt gaccacgccg gtacagcctt cggacgatgt cgttgtggtg
      661 gatacgcccg agccgttgct gtagctcaaa ctggtttcaa aaccaacatc atgcgagaat
      721 agtataccat cgccgaatac accgaagctg atggagaagt tgaaataaaa accgtgcgac
      781 atttcttcgg aaacggattt tatattgctt tttccccacg aaacctcgtt aagaccgccg
      841 ttatgtccga gtcgaatggc gagcttactg agctgctctg cacccaaatg gctgtaaacg
      901 ccccagccga gctgattgta cataaacggg ttgccctcgt ttccgtcaag ccagtttgcc
      961 gctttatcta tttttaccaa gggataatag tattttttat ctttgtctgt tttatccgat
     1021 tcccttatga cggcatgata gttcttgtta ttcatataac cgttatactc atcaacaaag
     1081 ccgttgtagt cgtcaacgct cagctgttga tataccggct gctgcggaat ggaaagcgtt
     1141 atggcggtat ctcccgacca tttgccgttt tcgtccagta tctcataggc gtagataaat
     1201 acaggcgttc tggacacaag caccgtgttg tacgcccgtg ccttaaccgt cagggaatat
     1261 tccttctgca gagccgtgga ataggttttg ctccattcaa gcgagtagcc tgccgagatg
     1321 ttcacctcaa cgggggcgag aagccctccg taagagtaag acattccgac gctgaaggaa
     1381 acattgttcg atgaggactg ctccaactcg aaactctctg tgattgtata tgcggtttcg
     1441 ttttcgccgg agtcactcag cgcggagaaa tacggcgcag cctgtatgac tgccttgacc
     1501 tccgggtcgc tgtaggcata gcttacgccc ttatatttgg ctataacacc gtcctggttt
     1561 ctgtcgcagg cggtgagaag tacctgagcc gtacagccgt agtaatcacc gtacgcttcg
     1621 ttcggactcc agtatgacaa tgtcaggtca aagccgtttg ccgcctcata gctgtcgctt
     1681 gtatccggat aggttgcgga aatcataccc ttgctgaagg aatatttatc ctcgttaccg
     1741 gtttcggcag aggttacaaa cacgatctgc tcgcgtcctg cattgttgcc gtcaaagttt
     1801 ccgacggcaa ccgagcggaa gaattctgtc ttaccgccga tctcgccctc aaaccagaag
     1861 ttgtttgttt tgccgtttga gtttgtgcgg aaagaatatt cgtttgaagt gagcttgtaa
     1921 agttctccgg tctgggcatt atagttgtac agcgtgccgt tgataaagac gaaatctgcg
     1981 gcgctcgcac cgttaaaagc aacagcctct accgctaaag gcgaaacaac ttctttagtt
     2041 ctggaaccgg ccttcgtcca accgttggaa gccatcacct tatattcgga ttgtacaaag
     2101 cctctgtttc cgttattaag gagcgttgta tatgcaattt cggatgaaga agtcgatgca
     2161 acagacaaag tgataccagc gacactggca cgtaagcccg caacaaggat atcttcatat
     2221 ccgtcgttgt tgaagtctcc tgccgtgatg ctgcaggcgt tcatgctgtt actattaaaa
     2281 acaacatcat tttgggaaaa attttggagt tctccctccg ggcaaacata gaatttttca
     2341 tcggcgtcgc cgtccacaaa cgaggagcct ccgccggaag aactaccgcc ggcttttcca
     2401 tatttaatct ttacctgagg acgatataga ttcaaattgc gactgtttac attgtttatg
     2461 aagaaatagg acagcacggc gatgtcatcg atttggtcgc cgttaaaatc gcccaccacc
     2521 atgtctacgc ccagcttatg aaatcggttg tcaaaggttt ggccagagct tccatacatg
     2581 ctgtcaccat ttgcccctgg gtccgtggga cttccaacag tggtgtgata taattttttg
     2641 taggagtcat gaagcatatc attgccgaag ccgagttttt tgccttctcc cataatgctt
     2701 gtgttattta ataatacaaa gctgtttttg ccggtaaagg aatattcata cagaaagcag
     2761 ccgacaaaag gttcgtaatc aaaagtctgc atcaaacctg tcgagcgttt tgtgtgcgga
     2821 acatagatga tcagcgtgtc cttgccgtct ccgtcaaagt cgcctgcggt aatgttcaga
     2881 taggcgttgg tttcaaggtt tagagcacga ataaggcctt tgttgtcaaa catccagttc
     2941 atttcgccga cccactgttt gcctttatat tctcccgttc ttgtatcata tatatgaaga
     3001 tagacctgct tatccgactt atcaagacct acataggcaa catggtctct cctgcctgag
     3061 ccgaacgggt caaaggaaac ggactggaca aacgcatagt acgtctgaag cgcactgatg
     3121 tccatattgg gcagagaggc atcggaagcg gttccttttt tggaaataaa gttcttgaga
     3181 gactccgggg ttcctacatc cgcaattttg cctgttgcgg acgtgccgta tgtctcaaaa
     3241 taaagcagtt cgtttttttc aaccattgca aagggacggc cgacatcata cccgtaaggg
     3301 ttggttgtat tgtcaaagcc agtgggtttt gtgtttttca tcagagtatt agcggcatcc
     3361 gatgaaaact gcggctcact cgggtcggcg gcaaagaccg acaggggcag acatactgcg
     3421 atcattatga ccgacagcag cgcgcatagc gagcgcttca gaatgagttt tttgtacata
     3481 gagagtcctc cttttctttt cacgggggaa gattgttttc gggcggggat ttgacgcagc
     3541 aaa
//
LOCUS       DBMDVO010000060         3528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11793_length_3528_cov_1.690400, whole genome shotgun sequence.
ACCESSION   DBMDVO010000060 DBMDVO010000000
VERSION     DBMDVO010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11793_length_3528_cov_1.690400"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="ACZPE2_01510"
     CDS             <1..770
                     /locus_tag="ACZPE2_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1318555.1"
                     /translation="VDSSTVNMNNLVYLGINDSKEVLSSSAVRTAIYYAIDKKDIASS
                     SYQGYATAAPLLFNPAFSELSGVTNVNFAESANADKAKSILTKTGYNKYAKDGSLTNG
                     SQNLKVSLLVNSENSFRAAAANSIAESLRSIGFSVTVDAVPLDKYNQKIASGDFDLYL
                     GEIKLTENLDLRPFFSEKGSASKGIDFSLRAVTDYEAFCDGSIGLSEFLDSFLNDMPF
                     VPVCYRSGFAAYKKGLKPDFSYSSDHLYAGICRWSLS"
     gene            872..1225
                     /locus_tag="ACZPE2_01515"
     CDS             872..1225
                     /locus_tag="ACZPE2_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1318556.1"
                     /translation="MIKIENYLGVIEIHQHYFSELIGQTVTSCFGVAGMANSGASQGL
                     RSLLFKRKNYPDKGVGVRADGGDLIIDLHIIVTYGVNIGTIVDSITNKVRYTVEQSTG
                     LGVKKVNVFVDGMKE"
     gene            1242..2915
                     /locus_tag="ACZPE2_01520"
     CDS             1242..2915
                     /locus_tag="ACZPE2_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1318557.1"
                     /translation="MLTGKQFKRAFISGANNILNHRKDVDALNVFPVPDGDTGTNMSM
                     TSSAAVRELQVVSDDEDLSIVAAKTAAALLRGARGNSGVILSLVFRGISNGFKGKKEV
                     GGKDLAAALTLGKESAYKAVMKPTEGTILTVVRKAAAAAEALALDDCGAVLDEAYRAA
                     QEALAETPELLPVLKEAGVVDAGGQGFLYILDGIRGYINEGKFIESNDDASPAKSNAE
                     ESKSVVAAASGDIKYGYCSEFLITKSKTPESTPLKLKAYLESIGDCVVVVDDDDIVKV
                     HVHSNEPGNVIQAALKIGQLINIKIDNMRYQHANADVGMDAKKDNKEENLSRDTEINR
                     VQPTKDYGFVAVAAGDGMAELFKELGCDVVVSGGQTMNPSTDDILNAVESTPAKHVFV
                     LPNNKNIIMAAEQTVNLADRGVSVVATKTVPQGITAMLNFDESLSSEENHLNMAKAAE
                     SVHTAQVTFAARDSVFGGQKIKEGQYLGMEDGKITLVEDDLVNAAYRVTRRLVKKFGG
                     SLITVFYGEDADENSANVLSARLQERFPNVEVNVINGGQPVYYFISSVE"
     gene            2954..>3528
                     /locus_tag="ACZPE2_01525"
     CDS             2954..>3528
                     /locus_tag="ACZPE2_01525"
                     /inference="COORDINATES: protein motif:HMM:NF028500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1318558.1"
                     /translation="MDFSTEIQYLKGVGPARAEAFSKLGVDTVGALLRFYPRAYEDWS
                     RVVPIREAPVDTECCIRAVVSYTPSKVRLPGGRLLSKTSVTDGIGILNLVFFNNKFID
                     SMLKEGEEYLFFGKITVDKHGIRQMVSPLISKGEDKDRIRPVYPQTADVSSKLTEKCT
                     MQALKGCKEIPDFMPSEILEKNDLCSLDYAIR"
BASE COUNT          975 a          777 c          901 g          875 t
ORIGIN      
        1 ccgttgacag cagtacggtc aatatgaaca accttgttta tctcggtatt aacgactcaa
       61 aggaagttct ctcatcgtcc gccgtcagaa ccgcaatata ttacgcaata gataaaaaag
      121 acattgcttc gtcgtcgtat cagggatatg caacagcagc tccgcttctg ttcaatcctg
      181 cgttttcaga actttcggga gttacaaacg tcaatttcgc cgagagtgca aatgccgaca
      241 aggcaaagtc catactgaca aaaacaggat ataacaaata tgcaaaggac ggctctctta
      301 caaacggctc acagaacctt aaggtgtcgc ttcttgtgaa cagcgaaaac agcttccgcg
      361 cggcggcggc gaacagcata gccgaaagcc tcagaagcat aggtttttcc gtgacagttg
      421 atgccgttcc gcttgataaa tacaatcaga aaatcgcttc cggtgatttt gacttgtatt
      481 taggagaaat caagcttacc gaaaatcttg atttgcgccc gttcttttcg gaaaaggggt
      541 ctgcgtcaaa gggtatagat ttctcgttgc gtgcggttac cgattacgaa gccttttgcg
      601 atggcagcat agggctttca gagttccttg acagcttctt aaacgatatg ccgtttgttc
      661 ccgtttgtta cagaagcggc tttgcggcgt ataaaaaggg gctaaagccg gatttctcgt
      721 attcctccga ccatctctat gcggggattt gccgctggag cttaagctga gccgccgttc
      781 ggatacaata ttgtatttgt gcggtttatg gtatataata acaacatcac aggaaaggag
      841 aatattgcgc ttttgcaata taaaccgatg tatgattaaa attgaaaatt atctcggcgt
      901 tattgagata catcagcatt atttttctga gcttatcggt caaaccgtaa cctcatgctt
      961 cggcgttgcg ggtatggcta acagcggcgc gtcacagggg cttcggtcgc ttctttttaa
     1021 acgtaagaat tatccggata agggcgtcgg cgtgcgtgcc gacggcggcg atttaataat
     1081 agatcttcac ataatcgtga cttacggcgt taatatcgga accatagtcg attcaataac
     1141 gaataaggtc agatataccg tcgagcagtc aacgggcctc ggcgtgaaaa aagtcaatgt
     1201 gtttgttgac ggaatgaaag aataatagga gtgtacaagt tttgttaaca ggtaagcagt
     1261 ttaaacgcgc tttcatttcc ggcgctaaca atatactgaa tcacaggaag gacgttgacg
     1321 cgctcaacgt atttccggta ccggacggcg ataccggaac aaatatgtca atgacctcct
     1381 cggcggcagt cagggagctt caggtggtaa gcgatgacga ggatttgagt atcgtcgcgg
     1441 ctaaaaccgc cgcagctctt ttgaggggcg caagaggaaa ttcgggtgtt atattatcgc
     1501 tcgttttcag aggtatttca aacggcttca agggtaaaaa ggaggtaggc ggcaaagacc
     1561 ttgccgcagc cctcactctc ggcaaggagt cggcttataa ggctgttatg aagcccaccg
     1621 agggtactat tttaaccgtt gtacgcaagg cggcggcagc ggcggaggca ctcgccttag
     1681 atgactgcgg cgctgttctc gacgaggctt accgcgcggc gcaggaggct cttgccgaaa
     1741 cacctgagct tttgcccgtt ctcaaagaag caggcgtggt agatgccggc ggccagggat
     1801 ttctctatat actcgacggt atcagaggct atataaacga gggcaagttt atagaaagca
     1861 atgacgatgc ctcgcccgca aagagcaatg ccgaggaaag caaatccgtc gtggcggcgg
     1921 cttcgggaga catcaaatac ggttattgca gcgagttcct cataaccaaa tcgaaaactc
     1981 cggagagcac accgctcaaa ttaaaggcat atcttgagtc gataggcgac tgtgttgttg
     2041 tggttgacga tgacgatata gtaaaggttc acgtccattc aaacgaaccc ggcaatgtaa
     2101 ttcaggccgc tctcaaaata ggtcagctca taaatattaa aatagataat atgcgttatc
     2161 agcatgcgaa tgccgatgtc ggtatggatg ccaaaaagga caacaaagaa gaaaatttaa
     2221 gtcgtgacac cgaaatcaat agagtacagc cgaccaaaga ttacggcttt gtcgcagttg
     2281 cggcaggtga cggtatggca gagctcttta aggagcttgg ctgtgatgta gtcgtttcgg
     2341 gcggtcagac tatgaatccg agcaccgatg atattttaaa tgccgttgag tcaactccgg
     2401 caaagcacgt ttttgttttg ccgaacaata aaaatattat aatggcggcg gagcaaaccg
     2461 taaatcttgc tgacaggggc gtcagtgttg tcgcaacaaa gactgttccg caaggcatta
     2521 ccgcaatgct caattttgac gagagccttt caagcgagga aaaccatttg aatatggcga
     2581 aggcggccga gagcgttcat acggctcagg tcacgtttgc cgcacgtgac agcgtgttcg
     2641 gcggacaaaa aatcaaggag ggtcagtacc tcggcatgga ggacggcaaa ataacccttg
     2701 tggaggatga ccttgtaaac gccgcataca gagttacacg ccgtcttgtc aagaaattcg
     2761 gcggctcgct tataaccgta ttttacggtg aggacgccga cgaaaactcg gcaaatgtcc
     2821 tttcggcgcg gcttcaggag cgtttcccca atgttgaggt aaatgtcatt aacggcggtc
     2881 agccggtcta ctacttcatt tcgtccgtcg aataacagga cgttttcaaa aacaattctc
     2941 ggatgtgttc ataatggact tttcaaccga aatacaatat ttaaagggtg tcggacctgc
     3001 tcgtgccgaa gcattttcaa agctcggcgt cgatacggtc ggcgcccttt tgcgctttta
     3061 tccgcgcgcg tatgaggatt ggagccgcgt tgtccccata agggaagctc cggttgatac
     3121 ggaatgctgt attcgtgccg ttgtcagcta cactccctct aaggtaagat tacccggagg
     3181 caggctgctt tcaaaaacaa gcgttacgga cggcataggc atattaaatc ttgtcttttt
     3241 taacaacaag tttattgaca gtatgctgaa ggagggcgaa gagtatctct ttttcggcaa
     3301 aatcacagta gataagcacg gcataagaca aatggtatcg ccgttgattt caaaaggaga
     3361 ggataaggac agaatacgtc ccgtttatcc gcaaacggct gatgtgagct ccaaactcac
     3421 ggaaaaatgc acaatgcagg cattaaaagg ctgcaaagag atacctgatt ttatgccgtc
     3481 cgaaatactt gagaaaaacg acctttgctc tcttgattat gcaatacg
//
LOCUS       DBMDVO010000061         3518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11845_length_3518_cov_1.411179, whole genome shotgun sequence.
ACCESSION   DBMDVO010000061 DBMDVO010000000
VERSION     DBMDVO010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11845_length_3518_cov_1.411179"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="ACZPE2_01530"
     CDS             complement(<1..700)
                     /locus_tag="ACZPE2_01530"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR1318559.1"
                     /translation="MDAQLGLWDANECFAELKPVKSADWKWTYADYPKKKNGLKVFSC
                     FACGGGSTMGYKLSGCDVLGCVEIDPKMNEVYVKNHNPKYNFLMDIRDFNKLPREEIP
                     EELFNLDILDGSPPCTTFSIAGDREDSWGKKKKFREGQCEQTLDDLSFVFIDTVAKLK
                     PKTVIMENVEGLLLGAAFGYVQRIYKQFSDIGYRVRHWLLKGETMGVPQKRHRVFFVA
                     VRNDIDFDLDSLDMS"
     gene            complement(713..1114)
                     /locus_tag="ACZPE2_01535"
     CDS             complement(713..1114)
                     /locus_tag="ACZPE2_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1318560.1"
                     /translation="MANFNFNTVIIGGRLCAAPEIKTTTNGTVVTSFDVAVSRQDNTD
                     FFRVVAWKNTAEFVCKYFGKGSSICVKGELQTRKYEAKNGEKRTATEIVARDVYFVDS
                     KGERAADKPANDTPEPPAQFEPISTDEELPF"
     gene            complement(1114..1821)
                     /locus_tag="ACZPE2_01540"
     CDS             complement(1114..1821)
                     /locus_tag="ACZPE2_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318561.1"
                     /translation="MEEIIKQWTTVAETDGNSDGSGYGDGSGYGYGYGSGYGYGDDSG
                     DGSGYGSGYGYGSGSGDGYGDGSGYGSGYGFGDGSGSGDGIKSFCGETVYIIDGIQTI
                     VKRIKGNLAKGYILNSDFTKTTCYVVKSDGCFSHGETLKKAREALQNKLFERMDKDET
                     ISEFRKKFKPGVKYPGEDYFVWHHYLTGSCEMGRRSFVNDREIDLSDKFTPEEFIEIC
                     KDAYGGEIIKQLGELYK"
     gene            complement(1809..2105)
                     /locus_tag="ACZPE2_01545"
     CDS             complement(1809..2105)
                     /locus_tag="ACZPE2_01545"
                     /inference="COORDINATES: protein motif:HMM:NF046222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6948 domain-containing protein"
                     /protein_id="HIR1318562.1"
                     /translation="MEKIMGKKVIVRGNRSGVFYGTLEEKNGTEVTLKDCRRLWYWEG
                     AASISQLAVSGTTKPRECKFTLTVPDIIITDAIEIIPCSKDAKKSIEGVPEWKK"
     gene            complement(2109..2324)
                     /locus_tag="ACZPE2_01550"
     CDS             complement(2109..2324)
                     /locus_tag="ACZPE2_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025456.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318563.1"
                     /translation="MKPQCRQVLDYMKRNGSITVQEAFRELSVGSLTSRISELKAAGI
                     DVRGSMERGINRFGDGVYYKRYYLGGN"
     gene            complement(2327..2452)
                     /locus_tag="ACZPE2_01555"
     CDS             complement(2327..2452)
                     /locus_tag="ACZPE2_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318564.1"
                     /translation="MLVHADEALNRRLKSEAQEKAMKVYGFGDKEWLDIFRRNWR"
     gene            complement(2535..3107)
                     /locus_tag="ACZPE2_01560"
     CDS             complement(2535..3107)
                     /locus_tag="ACZPE2_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NinB/YbcN family protein"
                     /protein_id="HIR1318565.1"
                     /translation="MELKTRGSYGEDALSFSRKGTQRLTISLSEDFRRYFDDFKDKDL
                     DIVIKPHREARSLNANALCWKLCTEIANVMRLSKEEVYLQALKDYGQSDVVSVRAGVD
                     VTPYFKYFELMGTSSVRGKEFNHYKVYRGSSEYDREEMGILLDGIVQEAKSLEIPVWS
                     KAEIEAIKEAWGEECFTEKTQMPHLRQHGG"
     gene            complement(3109..>3518)
                     /locus_tag="ACZPE2_01565"
     CDS             complement(3109..>3518)
                     /locus_tag="ACZPE2_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318566.1"
                     /translation="AAGTAYEHRILDFMGIRKKDRQIKIRRLKLRVNLDGEDRHTVHE
                     IKTHSADKPFTVSRAYWMQCQVEMFATGKKCVIDAYALLPEDYENYYNPIDPNRLEHF
                     PVEYDKSWIESKYLPELEYFADCLKKKIIPGGR"
BASE COUNT          776 a         1009 c          760 g          973 t
ORIGIN      
        1 aactcatgtc aaggctgtca aggtcaaaat cgatgtcatt tcggacggca acaaagaaaa
       61 ctctgtgccg tttttgagga acacccatcg tctcaccttt aagcagccaa tgcctgacac
      121 ggtatccgat atcggaaaat tgcttgtaaa tacgctgcac atacccgaaa gcagcgccaa
      181 gcagaagacc ttcgacattt tccattatta cggttttagg cttcagtttg gcgactgtgt
      241 caataaagac gaatgataaa tcatccagcg tctgttcgca ttgtccttcg cggaacttct
      301 ttttctttcc ccagctgtcc tccctgtccc ccgcaatgga aaaggtcgta cacggcggtg
      361 agccgtcaag gatatcaaga ttaaagagct cttcggggat ttcttcacgg ggcagcttgt
      421 taaagtcgcg tatatccatg agaaaattat atttcgggtt gtgattttta acataaacct
      481 cgttcatttt ggggtcaatc tctacgcatc ccaaaacatc acacccgctg agcttgtatc
      541 ccattgtgct gccccccccg caggcaaagc acgaaaacac tttcaagccg tttttctttt
      601 tgggatagtc ggcgtatgtc catttccaat ccgccgattt aacgggcttt aactcagcaa
      661 aacactcgtt tgcgtcccac aatcctaatt gtgcatccat ccatacacct cctcaaaacg
      721 gcaattcttc atccgtcgat ataggctcaa actgcgccgg aggctccgga gtatcgtttg
      781 caggcttgtc cgccgctctc tctcctttgg agtccacaaa gtacacatcc cgtgctacga
      841 tctccgttgc cgtgcgcttc tcaccgttct ttgcctcgta ttttcgcgtc tgaagctctc
      901 ccttgacaca tatggacgag cccttgccga aatacttgca gacaaactcc gccgtattct
      961 tccacgccac aacgcggaaa aagtccgtgt tgtcctgacg tgataccgcc acgtcaaacg
     1021 atgtgaccac agtgccgttt gtcgttgtct taatctcggg tgcggcgcaa agcctgcccc
     1081 cgataataac cgtgttgaaa ttgaaattcg ccattacttg tacaactccc ctaactgttt
     1141 gataatttcc ccaccgtatg catccttgca tatttcgata aactcctccg gagtaaactt
     1201 gtctgacaga tcaatttctc tgtcgtttac aaagctccga cgccccattt cgcacgatcc
     1261 cgtgagataa tgatgccaga caaagtagtc ctctccgggg tatttgacac cgggcttgaa
     1321 ttttttacga aattcggata ttgtctcgtc tttatccata cgctcaaaaa gcttgttctg
     1381 cagagcttca cgggctttct tgagtgtttc accgtgggag aaacacccat cggatttaac
     1441 aacatagcac gtggttttag taaaatcgct gtttaatatg taacctttag caagattgcc
     1501 cttaatccgc ttgacaatgg tctgtatccc gtcaatgata tatacggttt caccgcaaaa
     1561 agatttgatg ccgtcgccgg agccggagcc gtcgccgaag ccgtagccag agccgtagcc
     1621 ggagccgtcg ccgtagccgt cgccggagcc ggagccgtag ccgtagccgg agccgtagcc
     1681 agagccgtcg ccggagtcgt cgccgtagcc gtagccagag ccgtagccat agccgtagcc
     1741 agagccgtcg ccgtagccgg agccgtcgct gttgccgtcg gtttcggcta cagtcgtcca
     1801 ttgcttgatt atttcttcca ttccggtact ccctcaatgc ttttttttgc gtctttgctg
     1861 cacgggatga tctcaattgc atccgtaatg attatgtccg gtacagtcaa cgtgaacttg
     1921 cattctctcg gtttagtagt gccgcttacg gcaagctgtg atatagatgc cgcaccctcc
     1981 caataccata gacggcggca gtctttaagc gtaacctctg tcccgttttt ttcttcgaga
     2041 gtcccgtaaa atactcccga gcggttgcct ctgacgataa cctttttgcc cataattttt
     2101 tccatctttc aattccctcc taaataatat cttttgtagt aaactccgtc gccgaaacgg
     2161 tttatgccgc gctccatcga accgcgcaca tcaattcccg ccgccttcag ctccgatatg
     2221 cgggaagtaa gacttccgac cgaaagctct ctgaacgctt cctgcaccgt tatactgccg
     2281 ttgcgcttca tgtagtcaag tacctgcctg cattgcggtt tcatctttac ctccaattcc
     2341 gcctgaatat gtcaagccat tctttgtcgc cgaatccgta aaccttcatt gctttttcct
     2401 gtgcctcgct tttcagccgc ctgttaagcg cctcatccgc atgtaccagc atgtgatgct
     2461 tccggcacag atacactgtc agcccgtatt tgtcggaggc tctgcggtga gcgccctcga
     2521 atacgtgatg gcactcaacc gccgtgctgc cgcagatgag gcatctgcgt tttttctgta
     2581 aaacactctt caccccatgc ctccttgatg gcttctatct ccgctttgga ccatacggga
     2641 atttcaaggc ttttcgcctc ctgaacaatg ccgtcaagca aaatgcccat ttcctcgcgg
     2701 tcgtactccg acgaaccccg gtaaaccttg tagtggttaa attccttgcc gcgcacggag
     2761 gatgtgccca taagctcaaa atacttaaag tacggagtca catccacccc cgcgcgcaca
     2821 cttaccacat cgctttgtcc gtagtccttg agcgcctgca gatatacttc ctccttcgac
     2881 agcctcatga cgtttgcgat ttcggtgcac agcttccagc agagcgcatt tgcattgagg
     2941 ctgcgagcct ccctgtgcgg tttaatcact atgtcaaggt ctttgtcctt gaaatcgtca
     3001 aagtaccgtc gaaagtcctc agaaagcgaa attgtaagcc tttgagtccc ctttcgggag
     3061 aaagagagcg catcctcgcc ataagaaccg cgtgttttca gttccatgtt atcttcctcc
     3121 cggtataatc ttctttttga gacagtccgc aaagtattca agctccggca gatacttcga
     3181 ctcgatccag cttttgtcgt attcgacggg gaagtgctcc agcctgttcg gatcaatcgg
     3241 attgtagtag ttttcgtagt cctccggcaa aagagcgtat gcgtcaataa cgcacttttt
     3301 ccccgtcgca aacatctcaa cctgacattg catccagtaa gcccggctga cggtaaacgg
     3361 cttgtccgcc gaatgcgtct tgatctcatg caccgtgtgc ctgtcctctc cgtcgagatt
     3421 gacccgcagc ttcaatcggc gtattttaat ctgcctgtcc tttttgcgta tacccataaa
     3481 gtcgagtatg cggtgctcgt atgcagttcc cgcagcca
//
LOCUS       DBMDVO010000062         3508 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_11895_length_3508_cov_1.242149, whole genome shotgun sequence.
ACCESSION   DBMDVO010000062 DBMDVO010000000
VERSION     DBMDVO010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_3508_cov_1.242149"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="ACZPE2_01570"
     CDS             complement(<1..1263)
                     /locus_tag="ACZPE2_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024968.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1318567.1"
                     /translation="MKNMKVLFVTSGAIAYDVSSGNTFLNLFGELADTELYSLFTRSG
                     APDKKIIAAFRIAEAELPRYIFRRNRIGERVTASDCVLDEHAMQLEAVAKRNASQLKY
                     IARDILWKIAPWKNRNLRTFIEEVQPDLIVSILNENLSLGAMTAFAQKHSGAPLMLFA
                     WDDNYSYTVGWSAPLTRLSQTVHRRYMRKIAKRADTLFVISETLKADYERIFGRSCEI
                     MTKGADFSERPMPTPHHEPLRLLYAGNLALGRWETLAAIKEALQHINKNGMRAVLHIY
                     SATLLSDAQRDAIADGKNAVFMGVAPAAEIEKMQKDADILVHAESFDPQYYLSIHHSF
                     STKLVDCFHSAKCIFAVGPENAASLDCLIKNDAAIVAKSNDEIYEKLNLLINDKELID
                     AYAEKSWQYGKKSFDIKDIQELLLESMEK"
     gene            complement(1263..2237)
                     /locus_tag="ACZPE2_01575"
     CDS             complement(1263..2237)
                     /locus_tag="ACZPE2_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1318568.1"
                     /translation="MGFTEIKFPSDTLFLVTGAAGFVGSNLCEAILNMGYKVRALDDL
                     STGKQKNVDMFLGNPNYEFIKGDIKDLDTCMKACEGVDYVLNQAAWGSVPRSLEMPLF
                     YCKNNIEGTLNMLEAARQCGVKKFVYASSSSVYGDEPHLPKTEGREGNLLSPYALTKR
                     CDEEWAKLYTMHYGLDTYGMRYFNVFGRRQDPDGAYAAVIPKFLKQLMHGEVPTINGD
                     GKQSRDFTYIDNVIEANLKACLASHEAAGNAFNIAYGGREYLIDIYYGLTKALGVDVE
                     PNFGPDRKGDIKHSNADISKARKLLGYDPDYSFQDGIKLAIEWYKENL"
     gene            complement(2258..>3508)
                     /locus_tag="ACZPE2_01580"
     CDS             complement(2258..>3508)
                     /locus_tag="ACZPE2_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR1318569.1"
                     /translation="DWTGIFVVFVFDGDRVIGVQDCSGIKALYYGNIEGKVCFTSHPQ
                     LVADIYSLKMDPFVKKLVTNRFYNIGNRYLPGDLSPFSELKRIGANVYLEYFADNFEI
                     KRFYPVKPLELCKTQEEYELGIKKAYEILHKNIELASEKWESCAISLSGGTDSKTTLA
                     CANGLYDRFQFFSFQSKDTEITDSEAAHAICEKIGLKHNVYPIPAENSEIEDFDELKA
                     IIIHSYGYVRGLADNEIRKHICMYRWHYFDTEIKSWISEIVRVFFERKYGMKFPDRLT
                     PRHFSILQTRYFASPSLLRKSDKIYGEYMKKFDLEEPKFNFEHTDLYYWEVRMSSWGM
                     MVTQSLDLCHRITFPFNNRRLVELMLTLPREYRKSDKAHQDIIKYANKEIYDADIHIL
                     NNYFHSGRIMLEKIYFKYRTFLKK"
BASE COUNT          904 a          844 c          742 g         1018 t
ORIGIN      
        1 cttttccata ctctctaaaa gtaattcttg aatatctttt atatcgaagg actttttacc
       61 atactgccat gatttttcag catatgcgtc aatcagttcc ttgtcgttta taagcaaatt
      121 taatttttcg tatatttcgt cattactttt tgcaacaatc gccgcatcgt tttttatgag
      181 acaatccaac gacgccgcat tttcgggacc taccgcaaaa atgcattttg cactgtgaaa
      241 gcaatcgaca agttttgttg aaaacgagtg atgaatagag agataatatt gcgggtcaaa
      301 gctctccgca tgcaccagaa tgtccgcatc cttttgcatc ttttcgatct ccgcagcggg
      361 cgcaacaccc atgaacacgg cgtttttgcc atccgcaatg gcatcccttt gtgcatcgga
      421 cagcagcgtt gccgaataga tatgcagtac ggcacgcatg ccgttcttat tgatatgctg
      481 cagcgcttct ttgatggcgg caagcgtttc ccacctgccg agcgccagat tgcccgcata
      541 aagtagccta agcggctcat ggtgcggcgt cggcatcggc ctttcggaaa agtccgcacc
      601 tttcgtcatg atctcacagg aacgcccgaa aatacgctca tagtctgcct ttagtgtctc
      661 ggaaatgaca aacagcgtat ctgccctttt tgctatttta cgcatgtatc gccgatgcac
      721 cgtctgcgac aggcgcgtca gcggagcaga ccaaccgacg gtatagctgt agttatcgtc
      781 ccacgcaaac agcatcaacg gtgcgccgct gtgcttttgg gcaaaggcgg tcattgcgcc
      841 gagcgagagg ttttcattca gaatggaaac gataaggtcg ggctgtactt cctcaatgaa
      901 tgtccgcagg ttgcggtttt tccacggcgc gatcttccag aggatatcac gggcgatata
      961 tttgagctgg gaggcattgc gcttggcgac cgcctccaac tgcatggcat gctcgtcgag
     1021 tacacagtca ctcgccgtga cgcgctcgcc gatgcggttg cggcgaaaaa tgtagcgagg
     1081 cagctccgcc tccgcaatgc gaaaagcggc aattatcttt ttgtcgggcg caccgctgcg
     1141 ggtgaaaagg ctgtaaagct ctgtatccgc aagctcaccg aacagattga gaaaggtgtt
     1201 gccgctgctg acatcgtagg cgatggcgcc gctggtaaca aataacactt tcatgttctt
     1261 cattacaaat tctccttata ccactcgata gcaagcttga tgccgtcttg gaagctgtaa
     1321 tcgggatcgt agccgaggag ttttctcgct tttgaaatat cggcgtttga gtgctttatg
     1381 tcgccctttc tgtccgggcc gaaattcggt tcgacgtcaa cgccgagtgc ctttgtaaga
     1441 ccgtaataaa tgtctataag gtactctctg ccgccgtagg cgatattaaa cgcgttgcct
     1501 gccgcctcgt gagaagcaag acaagctttg aggttcgctt caataacatt gtcgatataa
     1561 gtaaagtctc ggctctgctt accgtcgccg ttgattgtcg gcacctcgcc gtgcataagc
     1621 tgttttaaaa atttcggaat taccgcggca tacgcgccgt ccggatcctg acgtctgccg
     1681 aatacgttaa aatatctcat tccgtatgta tcaagtccgt agtgcatggt ataaagcttt
     1741 gcccattcct cgtcgcatcg ctttgtaaga gcgtaaggag aaagcaaatt gccctctctg
     1801 ccctcggttt tgggtaggtg cggctcgtcg ccgtatacgg acgaagaaga tgcatataca
     1861 aactttttaa caccgcattg tcttgccgct tcgagcatat tgagcgttcc ctcaatattg
     1921 tttttgcaat aaaacagcgg catttcaagc gaacgcggaa cgcttcccca cgccgcctga
     1981 ttaagcacat agtcaacgcc ctcgcatgcc ttcatacaag tgtcaaggtc ttttatgtcg
     2041 ccttttataa actcatagtt cggattgccg aggaacatat caacattttt ttgcttgccc
     2101 gttgaaaggt catcaagtgc tctgacctta tagcccatat tgagtatcgc ttcacaaaga
     2161 ttcgagccga caaagcccgc cgcacccgtt acaagaaaca atgtatccga cggaaatttt
     2221 atttcggtaa aacccattta gattttctcc ttcttttcta tttttttaag aaagtacgat
     2281 acttaaagta aattttttca agcattatcc tgcccgaatg gaaataatta ttgagaatat
     2341 ggatgtcagc gtcgtatatt tctttgttgg catatttaat aatgtcctga tgcgccttgt
     2401 cgctttttct atattccctc ggaagtgtga gcataagctc cacaagccgc ctgttgttaa
     2461 agggaaatgt tattctgtgg caaagatcca gactttgagt taccatcata ccccaactgc
     2521 tcattcttac ttcccaataa tacaagtcag tgtgctcaaa gttgaatttt ggctcttcca
     2581 agtcaaattt tttcatgtat tcgccgtaga ttttgtcaga ttttctaagc agagacggcg
     2641 aagcaaaata tcttgtctga aggatactga aatgcctcgg cgtaagcctg tcaggaaatt
     2701 tcataccgta ttttctctca aaaaagaccc ttactatctc cgaaatccac gacttgattt
     2761 ctgtgtcaaa atagtgccat ctatacatac aaatatgctt tcttatctca ttgtcagcaa
     2821 gtccacgcac atatccgtat gaatgtataa ttattgcttt aagctcatcg aaatcctcaa
     2881 tctcgctgtt ttctgcggga atgggataaa cattgtgctt caagcctatc ttttcacaaa
     2941 tggcatgagc agcttcggaa tccgtaatct cggtatcttt ggactggaag ctaaaaaatt
     3001 gaaatctgtc ataaagtccg ttcgcacaag caagggttgt tttgctgtcc gtaccgccgg
     3061 aaagagaaat ggcacagctt tcccattttt cagaagcaag ctctatgttc ttatgtaaaa
     3121 tttcataagc ttttttaatg ccaagctcgt attcctcctg tgttttgcaa agctccaaag
     3181 gctttacggg ataaaaacgt tttatttcaa aattatctgc gaagtattca agataaacat
     3241 ttgcgcctat ccttttaagc tcgctaaacg gcgacaaatc acccggcaaa tagcgatttc
     3301 ctatattata aaagcgattt gttaccagct ttttaacgaa tggatccatt ttaagcgaat
     3361 aaatgtctgc aaccaattga gggtgagagg taaagcatac cttaccctcg atattaccgt
     3421 aataaagagc ctttatgccc gaacagtcct gtactcctat tactctgtcg ccatcaaaga
     3481 caaaaacaac aaaaattcct gtccaatc
//
LOCUS       DBMDVO010000063         3456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_12157_length_3456_cov_1.528465, whole genome shotgun sequence.
ACCESSION   DBMDVO010000063 DBMDVO010000000
VERSION     DBMDVO010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12157_length_3456_cov_1.528465"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="ACZPE2_01585"
     CDS             complement(<1..2456)
                     /locus_tag="ACZPE2_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1318570.1"
                     /translation="MSAFAEVLKTSPEYKTLEKAVKSKNLPVGVLGVSGIHKAHIINS
                     LCGNLDRRALVIMPDEAAATKLCEDLRVFGKNSFLYPARDFSFTSAENRSHEYEQRRI
                     NALTNILNGTADIVVCSAEAAVQRTIPPEDLKKHSLTIRRGEDIKLGDITSVLLSAGY
                     TRADMVEGVGQFSVRGGILDLFSPDDQNPVRIEFWGDTVDTMAHFDIMSQRRTDNVDK
                     LTLMPSTEIVFSGGDDLMKKITAFYDTVKGKGAKKAREMLQKDIDRLKNGVSLSSADK
                     YLPLAYEENATILSYADSLLLIVCESAGVKEKANTAIKLQNEDIKYLLEDGELCRGLD
                     EFTMTFPELTREYEKKDTVFLDDFARGSFDVPVRDLVTFTASTLPVWNGKLDILLDDL
                     SPLVQKDSTVVVMGGTEKAAKNLAEDLENEDVPALYFPLEPAEFPRGRVSVLPGALSG
                     GFGYPHEKFYAFTYGRNAVASKKRKSRSGFKKGQSLHSLDELKKGDYVVHSLHGIGVF
                     DGINKLEVGKITKDYIKIKYAKGDVLYVPVTQLDLVSKYIGPHEDDGRTVKLNKIGGK
                     DWEKTRNKVRAAVKDMADQLIALYSKRQNSPGYAFSPDIDMQSDFERRFEYDETSDQL
                     RAIDEIKGDMEKPYPMDRLLCGDVGFGKTEVALRAAFKCAADGKQCAILVPTTILAYQ
                     HYRTILKRFDGFPVEIEMLSRFRTPKEQEKILKNLKRGSLDIIVGTHRLVSKDVKFKD
                     LGLLIVDEEQRFGVAQKEKLKELFPTVDVLTLSATPIPRTLNMAMTGIRDMSVIEEAP
                     QDRYPVQTYVIEHDMGILCEA"
     gene            complement(2474..3079)
                     /gene="pth"
                     /locus_tag="ACZPE2_01590"
     CDS             complement(2474..3079)
                     /gene="pth"
                     /locus_tag="ACZPE2_01590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1318571.1"
                     /translation="MLGRFKKTEAVGAPEFLVAGLGNPEKKYTLTRHNSGFLCVDFLA
                     EKHGFKINKLKFKAVIADTVINSHRCIVMKPQTYMNLSGEAVKAAADFYKIPPEKIIV
                     IFDDISLDVGRLRIRRKGSDGGHNGIKSIISCIGSQNFPRVKIGCGKKPHPDYDLAAW
                     VLSEYKKDEAPELKKALDNACCAVELILDGKTDEAMNKYNS"
     gene            complement(3098..>3456)
                     /locus_tag="ACZPE2_01595"
     CDS             complement(3098..>3456)
                     /locus_tag="ACZPE2_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR1318572.1"
                     /translation="VIGEKLHLGNFVEVKNSNIDTGTKVSHLTYVGDSDVGKRVNFGC
                     GTVTVNYTGKAKFRTTIGDDAFIGCNTNLVAPVTVGDGAYTAAGSTITEDVPGDSLGI
                     ARARQVNKIGWCLKDK"
BASE COUNT          804 a          895 c          770 g          987 t
ORIGIN      
        1 gcctcgcaga gaattcccat atcatgctct ataacatatg tctgaacggg gtatctgtcc
       61 tgcggagcct cctctattac ggacatatcg cgtatgccgg tcatagccat attaagcgtt
      121 ctcggaatag gcgtcgccga caaggtcaaa acatcaaccg ttgggaacag ctccttgagc
      181 ttctcctttt gagccacgcc gaagcgctgc tcctcatcga ctattaaaag gccgaggtcc
      241 ttaaatttta catcttttga aacaagacgg tgtgtgccta cgattatgtc aagcgagccg
      301 cgcttcaaat ttttaagtat tttctcctgc tccttaggag ttctgaagcg tgacagcatc
      361 tctatctcga cagggaaacc gtcaaatctt ttaagtatgg tacggtaatg ctgatacgca
      421 agaattgttg tgggaacaag tattgcgcat tgcttaccgt ctgccgcgca tttaaaagcc
      481 gcacgaagcg cgacctcggt ttttccgaag ccgacgtcgc cgcatagaag tctgtccatg
      541 ggataaggct tttccatatc gccctttatt tcatcgattg ctctgagctg atcggacgtc
      601 tcgtcatatt caaaccgccg ttcgaaatcg ctctgcatat ctatatccgg agaaaatgca
      661 tagccggggc tgttttgcct ttttgaatac agggctatga gctgatccgc catatccttg
      721 actgcggcac gaaccttatt tctcgttttt tcccagtcct tgccgccgat tttattcagc
      781 tttactgttc tgccgtcgtc ctcgtgaggg cctatatatt ttgagacaag gtcaagctgc
      841 gttaccggga cataaagaac gtcgcccttt gcatacttga ttttgatata atccttggtt
      901 attttgccga cctcaagctt attgatgccg tcaaacacgc ctataccgtg gagcgaatga
      961 acaacgtagt cgcccttttt aagctcatca agcgagtgga ggctctgtcc ctttttaaac
     1021 ccactcctgc tttttctttt tttggacgca acggcgtttc tgccataggt aaaggcataa
     1081 aacttttcgt gcggatagcc gaagccgccc gagagcgcac cgggcaaaac cgaaactctg
     1141 ccacgcggaa attcggcggg ctcgagcgga aaataaagtg ccggaacatc ctcgttttcg
     1201 agatcctcgg caaggttttt tgcagccttt tccgttcctc ccattactac aaccgtcgaa
     1261 tccttttgaa caagcggaga caaatcgtcc aagagtatgt cgagctttcc gttccatacg
     1321 ggaagtgttg acgccgtaaa cgttacaagg tcgcgtaccg gtacgtcaaa cgaaccgcgc
     1381 gcaaaatcgt cgagaaaaac agtatccttt ttttcatatt cgcgtgtaag ctccgggaag
     1441 gtcatcgtaa actcgtcgag tccgcggcaa agctcgccgt cctcaaggag atatttgata
     1501 tcctcgtttt ggagctttat tgcggtgttt gccttctcct tgactccggc actctcgcaa
     1561 acaataagca aaagcgaatc ggcatagctt aaaattgtcg cattctcttc gtaagcgagc
     1621 ggaagatact tatcggcaga gcttaagctc acgccgtttt tgagcctgtc tatatccttt
     1681 tgcagcattt cgcgagcctt tttggctccc ttacccttta cggtatcata aaatgcggta
     1741 atctttttca ttaagtcgtc gccgcccgaa aagactatct cggttgacgg cataagcgtc
     1801 agcttatcaa cattgtcggt tcttcgctgc gacattatgt cgaaatgagc catggtgtca
     1861 acggtatcgc cccaaaactc tattctgacg ggattttggt cgtcgggcga aaacaggtcg
     1921 agtattccgc cgcgcacgga aaactgacct actccctcca ccatatcggc tctcgtatag
     1981 cctgcggaaa gaagcacact cgttatgtcg ccgagcttaa tatcctcgcc tcgccttatc
     2041 gtaagcgaat gtttttttaa gtcctcgggc ggaatagtcc tctgaaccgc cgcctctgcg
     2101 gagcaaacca ctatatcggc ggtgccgttt aaaatatttg tgagcgcatt tatacggcgc
     2161 tgttcatatt cgtgggaacg attttccgcc gaggtaaacg aaaagtccct cgccggatac
     2221 agaaacgaat tcttaccgaa aacacgcaag tcctcgcaaa gcttcgtcgc agccgcttcg
     2281 tcgggcataa tgacaagagc cctgcggtct aaattaccgc agagcgaatt tataatatga
     2341 gccttatgta ttcccgaaac gccgagaact ccgacaggca gatttttgct ttttaccgcc
     2401 ttttcgagcg tcttatattc gggcgaggtc tttaagacct cagcaaatgc tgacatatta
     2461 aattaaagct ccttcaggaa ttatatttat tcatggcttc gtctgttttg ccgtccaaaa
     2521 taagctccac cgcacagcag gcattatcaa gtgccttttt aagctccggg gcctcgtcct
     2581 ttttgtactc ggagagaacc catgcggcaa ggtcgtaatc gggatgaggc tttttgccgc
     2641 agccgatttt gactctcgga aaattttggc tgcctataca ggatattatg ctctttatac
     2701 cgttatgacc gccgtctgag cccttgcggc gtattctcag tctgcctacg tcaagcgata
     2761 tatcgtcaaa aatcacaatg attttttccg gaggtatctt ataaaaatct gccgcagcct
     2821 ttacggcctc tccgcttaaa ttcatatagg tctgcggctt cattacaatg cagcggtgcg
     2881 agtttataac cgtatcggct ataaccgcct tgaacttgag tttatttatt ttaaaaccgt
     2941 gcttttcggc aagaaagtca acgcacaaaa accccgaatt atgacgtgtc agggtatatt
     3001 ttttctcggg gttaccgagt cctgccacta aaaattcggg tgcgccgaca gcttccgttt
     3061 ttttgaatct gcctaacatt attgtgttta cctgtcctta tttatctttt aggcaccagc
     3121 ctatcttatt aacctgccgt gctcttgcta taccgagcga atcgccggga acgtcctctg
     3181 ttattgtgga gcctgccgcg gtatatgcgc cgtcaccgac cgtcacggga gctacaagat
     3241 ttgtgttgca gcctataaac gcatcgtcgc ctatggtggt tctgaatttc gccttgcctg
     3301 tatagttgac cgttaccgtt ccgcagccga agtttaccct cttgcccacg tcgctgtcgc
     3361 cgacatacgt aaggtgcgaa acctttgtgc cggtatctat attggaattt ttcacctcga
     3421 cgaagttgcc gagatgcaac ttttcgccta tcaccg
//
LOCUS       DBMDVO010000064         3439 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_12250_length_3439_cov_0.981426, whole genome shotgun sequence.
ACCESSION   DBMDVO010000064 DBMDVO010000000
VERSION     DBMDVO010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12250_length_3439_cov_0.981426"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="ACZPE2_01600"
     CDS             <1..235
                     /locus_tag="ACZPE2_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318573.1"
                     /translation="TVITGTQHLDRGYEAPEKVLKLLGADVRRINENDGTEKQETRQF
                     AENIGKPAGESAEDCTDRHSQEADDRCNIRRGK"
     gene            225..1073
                     /locus_tag="ACZPE2_01605"
     CDS             225..1073
                     /locus_tag="ACZPE2_01605"
                     /inference="COORDINATES: protein motif:HMM:NF020067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1318574.1"
                     /translation="MANERTQGGRQQLSRDEMRRRSRLIKMKKRRIKKLIALILGIII
                     IIAVAVTLSLTVFFRIDNVAVTGDEIYNNEDVIAASQINIGDNMFMTSKKKTASGIEC
                     TLPYVESAEIKRNLSGTITIKITAATAKLAIDNGDSFTLVSDKGKVLEDGLMTIGDGI
                     IIIDSSPVISAVPGETVEFQDNDDLGIIKNVLTLLESNELSGITSIDVSNHSNIKMVY
                     KERVTVLLGLSSSVNDKMDFVKAALTRLDSDNPDFTGSIDFTIDKKAYLSPQREDNTT
                     VPFISP"
     gene            1205..2323
                     /gene="ftsZ"
                     /locus_tag="ACZPE2_01610"
     CDS             1205..2323
                     /gene="ftsZ"
                     /locus_tag="ACZPE2_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1318575.1"
                     /translation="MDFEMDNEFNDVTNIKVIGVGGGGGNAVNSMANNDFGRVQLIAI
                     NTDKQTLAFSKAGQKIQIGEKITQGRGAGAKPEIGQKAAEESRDAIIEALNDTQMVFI
                     TAGMGGGTGTGAAPVIAEIAKEKGILTVGIVTKPFDFEGKVRMQQAEAGIAELAGHVD
                     SLVVIPNERLKQVSEQKITLLNAFDIADDVLRQGVKSISDLIIKPALINLDFADVTAV
                     MKDAGHAHMGVGTAKGKDKAEQAAQRAISSPLLESTIDGAKGVIISISASEDIGLDDI
                     DTAAGIVRAAADEDANIIFGVDLDKEKEDEMTITVIATGFGTDENGMPKKPNKTDDFS
                     FGGKDDGFEDVSSTTDNGKGFGSDDGYMDVLDIFNGKK"
     gene            complement(2472..3041)
                     /locus_tag="ACZPE2_01615"
     CDS             complement(2472..3041)
                     /locus_tag="ACZPE2_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR1318576.1"
                     /translation="MLNLGFAMCGSFCTFKKVIPIMAKLKSDGFNVVPIMSETAFTTD
                     TRFGSAKDFADEITEITGNDIISTLPKAEPIGPKKLLDLLIIAPTTGNTLGKMANGIT
                     DTTVTLAAKAHLRNRRPVVIAVSTNDALGATAKNIGLLMNCKYIYFVPMRQDDCINKP
                     NSIVADFEKIPITVLDALNGKQTEPVIIS"
     gene            complement(3054..>3439)
                     /locus_tag="ACZPE2_01620"
     CDS             complement(3054..>3439)
                     /locus_tag="ACZPE2_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012875.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR1318577.1"
                     /translation="YGRTAKAIAEFMKENGAKVLITARDPHALADAVKKGYSICLLRD
                     YDCIAHDADILINTVPAEIIDKNILSRLRKDVLLIDIASPPFGVDIGLADSYGINAVR
                     ALGLPGKYAPEEAGRLIFKKAQPLL"
BASE COUNT          984 a          719 c          839 g          897 t
ORIGIN      
        1 gacggttatt acaggtacgc agcatttgga cagaggatat gaagctcctg aaaaggtgct
       61 gaaactgctc ggagcagacg tgagaaggat aaatgaaaat gacgggaccg aaaaacaaga
      121 gacaaggcag ttcgccgaaa acatcgggaa accagcgggg gagtcggcag aagactgcac
      181 cgaccggcac tcgcaggagg cagacgatcg atgtaacata cggcgtggca aatgagcgca
      241 cgcagggcgg caggcagcag ctctcgcgcg atgaaatgcg ccgtcgcagc aggctcatta
      301 aaatgaaaaa acgcaggata aaaaagctga ttgctcttat cctgggtatt ataattataa
      361 ttgccgtcgc agtaactctt tcgcttacgg tctttttccg tatagacaat gttgcggtta
      421 ccggtgacga gatatacaac aatgaggatg ttatagctgc gtcgcagata aatataggcg
      481 acaatatgtt tatgacaagc aagaaaaaaa cagcctccgg aatcgaatgc acccttcctt
      541 atgttgagtc ggccgaaata aaacgcaatc tttcgggaac aataacgata aaaatcacgg
      601 ctgcaaccgc caagcttgca atagacaacg gcgacagctt tacgttggtt tccgacaagg
      661 gcaaagtgct tgaggacgga cttatgacta tcggcgacgg tattatcata attgattcct
      721 caccggtgat ttcggcggta ccgggggaaa ccgtggaatt tcaggataac gacgacctcg
      781 gcattataaa aaatgtactg acattgcttg aatcaaatga actcagcgga ataacaagta
      841 tagatgtgag caaccattcc aatattaaaa tggtatataa ggagcgcgtt accgtgcttt
      901 tgggactttc gtcctccgtg aacgataaaa tggactttgt aaaagctgcc ctgacaaggc
      961 ttgacagcga taatccagat tttacgggca gcatagattt cacaattgat aaaaaggcgt
     1021 atctcagtcc tcagcgtgag gataacacaa cggttccgtt tatatctccg taattatata
     1081 caatataaat gcaaaaaaac gttgaaaata acgggataat atgatatact aacctaaata
     1141 ggtatgagtt taactttatt taggtgtgaa ataaaagact ttacataatg gaggatgttc
     1201 tcaaatggat tttgaaatgg acaacgaatt taatgacgtt actaatatta aagttatagg
     1261 cgttggcgga ggcggcggaa atgctgtcaa cagtatggca aacaatgact tcggcagagt
     1321 tcagcttata gctataaata ccgataagca gacgcttgca ttttcaaaag ccggtcagaa
     1381 aattcagata ggcgaaaaga ttacgcaggg cagaggcgcc ggagcaaagc ccgaaatcgg
     1441 tcagaaagct gccgaggaga gccgcgacgc aattattgaa gcgctcaatg atacacagat
     1501 ggtgtttatt accgcaggta tgggcggcgg aacaggcaca ggcgcagctc ccgttattgc
     1561 cgagattgct aaggaaaagg gaatactcac cgtaggtata gttacaaagc cattcgattt
     1621 tgagggtaag gtacgtatgc agcaggcaga ggcaggtatt gcagagcttg caggccatgt
     1681 agacagcctt gttgttattc ccaacgaaag attaaagcag gtatccgagc agaagattac
     1741 tcttctcaat gcgtttgata tagcagatga tgttcttcgc cagggcgtta agagtatttc
     1801 cgaccttatt attaagccgg ctcttataaa tcttgacttt gccgatgtaa ccgcagttat
     1861 gaaagatgcg ggccatgctc atatgggcgt gggtaccgcc aaaggcaagg ataaggctga
     1921 gcaggcggct cagagagcta tttcaagccc tcttcttgaa tccacaatcg acggcgctaa
     1981 gggcgttata ataagcattt ccgcttctga ggatatcggc cttgacgata tcgacaccgc
     2041 ggcaggtata gttcgtgcag cggctgatga ggatgctaac atcatcttcg gcgtagatct
     2101 cgataaagaa aaagaagacg agatgacaat taccgttatt gcaacaggct tcggtacgga
     2161 tgagaacggt atgcctaaaa agcccaataa gaccgatgat ttttcgttcg gcggcaagga
     2221 tgacggcttt gaggatgtaa gcagcactac cgacaacggc aagggcttcg gaagcgacga
     2281 cggatatatg gacgttcttg acattttcaa cggtaagaag taatttttta cacagtattt
     2341 tttcttatta aatcccttaa aaaacgggct gtaaaaattt tacggtccgt tttttgtcga
     2401 aaaaaatatc cgcactcgat atttaacgaa tgcggatatt ttagttgtgc cattcttctc
     2461 tttgtgtgac tttacgatat tattacgggc tcagtctgtt tgccgtttaa agcatccaaa
     2521 acggttatgg gtattttttc aaaatccgca actatggaat tcggtttgtt aatgcagtca
     2581 tcctgcctca tcggtacgaa gtaaatatat ttgcagttca ttaaaagacc tatgttttta
     2641 gcggtggcac cgagcgcgtc atttgtggaa acggctatta ccaccggacg tctgttgcga
     2701 aggtgcgctt tagctgcaag cgttacggtc gtatcagtta tgccgttcgc cattttcccg
     2761 agagtgtttc ccgtagtcgg tgcgataata agtaagtcga gcagcttttt gggaccgatc
     2821 ggctcagcct tcggcagagt acttataata tcatttcccg ttatttcggt tatttcgtcg
     2881 gcgaagtctt tagccgagcc gaaacgtgta tcggtcgtaa atgccgtttc agacattatg
     2941 ggcactacgt taaacccgtc gcttttaagc ttagccataa tcgggatcac ctttttaaac
     3001 gtacagaacg aaccgcacat tgcaaatcct aagttcagca catttttccc tccttacaga
     3061 agcggttgag ctttcttaaa tatcagcctg ccggcttctt cgggggcgta ttttccggga
     3121 agccccaatg ctctgacagc gtttatcccg taagagtcgg caagcccgat atccactccg
     3181 aacggggggg aagctatatc tataagcaga acgtcttttc tcaatcgact caaaatattt
     3241 ttatctatta tttcagcggg aacggtgttt attaaaatat ccgcgtcgtg tgctatgcag
     3301 tcataatctc tcagcaggca tatgctgtat ccctttttga ccgcgtctgc gagtgcgtga
     3361 gggtctcttg ccgttatcag tacctttgcg ccgttttcct tcatgaattc ggcaatagcc
     3421 ttcgcggttc tgccgtatc
//
LOCUS       DBMDVO010000065         3390 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_12542_length_3390_cov_1.099726, whole genome shotgun sequence.
ACCESSION   DBMDVO010000065 DBMDVO010000000
VERSION     DBMDVO010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12542_length_3390_cov_1.099726"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="ACZPE2_01625"
     CDS             complement(<1..775)
                     /locus_tag="ACZPE2_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014443.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR1318578.1"
                     /translation="METKKTEKKRKFKLSPMKKIILGYIAIIFLGAFILTLPISSRDG
                     IHTSFEDALFTSTSATCVTGLVVQDTATYWSLFGQIVILCLIEIGGLGFMTLAVCAMT
                     FTKKNIGLQSRYTLLESINAPQMAGIVKMTRFTIRGAFLIEGVGALLLATRFVPRFGV
                     LKGIYFSVFHSVSAFCNAGFDIMGTPGNEYTSLTEYSADIVVNITVMLLIVIGGLGFF
                     VWADLMNTKFIFRRLKLHSKIVLVTTAMLIVVGAGLILIF"
     gene            complement(744..1220)
                     /locus_tag="ACZPE2_01630"
     CDS             complement(744..1220)
                     /locus_tag="ACZPE2_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1318579.1"
                     /translation="MTYFIQILTGTLGAFGFAILYNLRGTKLLIATLGGALSWGTYLF
                     FGIWFHSEPLRYFFAAIAVTIYAEIFARIKKTPTSTFLIVPMIPLIPGGVLYYTMSYA
                     LKNDWNNFVTSAFYTIKLAMALAVGIIVVSTLVRMFSAVLKFLRDKNGNKKDRKET"
     gene            complement(1217..1981)
                     /locus_tag="ACZPE2_01635"
     CDS             complement(1217..1981)
                     /locus_tag="ACZPE2_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1318580.1"
                     /translation="MDSKMLLTCALDIGEKMLISGAEISRVEDSVKRICNAYDVKRID
                     VFTITSSMVATLEDKDGNSITETRRITKHHTDLTKLHKLNDLSRKIVRRVPDIHYIRN
                     QIDEIEKGTKEYNLPIQCTVSALIAGAFAIFFGGAFLDGIAAALIGIVLKLIVYATEK
                     TQVNMIFANVVCSFAVCSIAFAFVMLGFGYSTDKIIIGNIMLLIPGVALTNSIRDMIS
                     GDIMAGMLRFCEACLVSLAIAAGYIIAALIFGGIGK"
     gene            complement(2134..2691)
                     /locus_tag="ACZPE2_01640"
     CDS             complement(2134..2691)
                     /locus_tag="ACZPE2_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1318581.1"
                     /translation="MEFDAFDEGIELGGLRNRDEIRLLICYILRAVDAPISKQNLNDS
                     MLQDGLANYFEINQAISELLKNGTIDMDIDENGEELLTATEKGKDIADTLETSLPKTV
                     REKAVNSAIKLMTLAKTMRENKIETVSAPGGGYYVTFSLMNGDEALMKLTIFVADSMQ
                     LEAVKNNFLNDPVKLYSSIITALTI"
     gene            complement(2746..>3390)
                     /gene="alr"
                     /locus_tag="ACZPE2_01645"
     CDS             complement(2746..>3390)
                     /gene="alr"
                     /locus_tag="ACZPE2_01645"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1318582.1"
                     /translation="DKTSCNNQKKKYLDFVGKLDKAGVEIPIKHMCNSAGIIEFDDNF
                     LDMVRSGIMTYGLYPSEEVDKSKLELHPALELKSHIAYIKSVGPGFTVSYGSTFTSKS
                     NMRIATVPVGYGDGYPRALSNKGKVLIHGKFADIIGRVCMDQFMVDVTDIPEAKQGDE
                     VTLIGKDQNNVITVEDVADNAHSFNYEFCCGINRRVPRVYYKDGKYLETVDYLD"
BASE COUNT          926 a          755 c          701 g         1008 t
ORIGIN      
        1 caaatataag tatcagacct gcacctacga ctatcaacat ggcagttgtg acaagaacga
       61 tttttgaatg aagcttcaat cttctgaata taaattttgt gttcataaga tccgcccata
      121 caaaaaagcc gagtccgccg attacgatga gcagcataac agtaatgttg actactatat
      181 cggcactgta ttcggtgagt gaggtgtatt cgtttcccgg tgtacccatt atgtcaaatc
      241 cggcgttgca aaatgcggaa acagagtgaa atacggaaaa ataaattccc tttaagactc
      301 cgaatctcgg aacaaatctc gttgcgagca aaagtgctcc tacgccttct atgagaaatg
      361 ctcctcgaat cgtaaagcga gtcattttga ctattccggc catttgaggc gcatttatcg
      421 attccaaaag agtgtatctg ctttgaagtc ctatattctt ttttgtaaat gtcatagcgc
      481 agactgcaag agtcataaaa cccaagccgc ctatctcgat gagacagagt ataacaattt
      541 gcccgaagag cgaccagtat gtggcggtgt cctgtactac aagcccggta acacacgtag
      601 ctgaggtgga tgtaaacagc gcatcctcaa acgatgtgtg gattccgtct ctcgaagaaa
      661 tgggcagcgt cagaataaac gcccccaaaa atattatagc tatatagccg agtattattt
      721 ttttcatcgg cgagagcttg aatttacgtt tcttttctgt cttttttgtt tccattttta
      781 tcccttaaaa attttaatac cgccgagaac atcctgacga gtgtggatac tactattatc
      841 cccaccgcaa gagccatagc gagctttatg gtgtaaaatg cgcttgttac aaaattgttc
      901 caatcatttt tcagggcata gctcatggta tagtagagaa cgccgccggg aatgagagga
      961 atcattggta ctatcaaaaa tgtcgatgtc ggagtctttt taattcttgc aaatatctct
     1021 gcataaatcg taactgcgat tgccgcaaaa aaatacctga gcggctcgct gtgaaaccat
     1081 attccgaaaa ataaatatgt gccccaggat aaagctcctc cgagggttgc gataagcagt
     1141 ttagtgcccc tcagattgta aagtatcgca aatccgaagg ctcccaacgt gccggtcaat
     1201 atctgtataa aatatgtcat ttgccgatac ctccgaaaat gagggccgca atgatgtaac
     1261 cggcggctat tgcaagcgat acaaggcacg cttcgcaaaa tctgagcatt ccggccatta
     1321 tgtctccgct tatcatatcg cgtatggagt tagtcagtgc cactcccggt atcagcagca
     1381 ttatattgcc tattattatt ttgtcggttg aatagccgaa gccgagcatt acaaaggcaa
     1441 aagctatcga gcataccgca aaagagcata caacattggc aaatatcata tttacctgag
     1501 ttttttccgt ggcatataca atcagcttca gtacaattcc tatcagtgcc gcagcaattc
     1561 cgtccaaaaa ggcgccgccg aaaaagattg caaacgctcc ggctatcagg gcgcttacgg
     1621 tacattgtat cgggaggttg tattccttgg tgcctttttc tatctcatct atctgattgc
     1681 ggatatagtg aatatcgggg actcttcgta ctattttcct tgacaggtca tttagcttgt
     1741 gaagctttgt taagtcggtg tggtgttttg taatgcggcg cgtctccgtt attgaattgc
     1801 cgtccttgtc ctcaagtgtt gcaaccatgc ttgaggttat ggtgaaaaca tcaattcttt
     1861 taacgtcgta tgcattgcag attcttttta cggaatcctc aacacggctg atttccgcac
     1921 cgcttataag cattttttct cctatgtcga gagcacacgt gagaagcatt tttgaatcca
     1981 taaaacacct ccgatttttg tacaaactga ttataatata ataagaagca aaaaacaatg
     2041 ctttttaaaa acgtttacaa ggacttaaaa gagcaaagca aaaccgcaaa agtctcggct
     2101 ttcgcggttc ttttaaataa aggtaaataa aggttatatg gtgagtgccg taataatgct
     2161 tgaatatagc tttaccgggt cgtttaagaa attgtttttg accgcttcaa gctgcatact
     2221 gtccgctaca aatatcgtga gcttcataag tgcctcgtca ccgttcataa gcgagaatgt
     2281 gacataatag ccgccgcccg gagcggatac ggtttctatc ttgttttctc gcattgtttt
     2341 agcgagcgtc ataagcttta tagcagaatt tacggctttt tcacggacgg ttttgggaag
     2401 tgaggtttca agcgtgtcag ctatgtcctt tcccttttcg gtagccgtga gcagctcttc
     2461 gccgttttcg tcaatatcca tatctatggt gccgtttttc aaaagctccg atatagcctg
     2521 atttatttca aaataattgg caaggccatc ctgaagcatg gaatcgttga ggttctgttt
     2581 cgaaatcgga gcgtcaacgg ctctcaaaat atagcatatc aaaagtctga tttcgtcgcg
     2641 gttacgaagc ccgccgagct ctataccctc gtcaaaagcg tcaaattcca ttttgcacct
     2701 ccgaaaaaat cacttctttt attatagccg aaagtgcatt acgtgtcaat caaggtaatc
     2761 tacggtctca agatattttc cgtccttata ataaacgcgc ggaaccctgc ggtttattcc
     2821 gcagcagaac tcataattaa agctgtgagc gttatcggca acgtcctcta cggttattac
     2881 attgttttga tccttgccga taagcgttac ctcatcaccc tgctttgctt cgggaatatc
     2941 cgtgacatcg accataaatt gatccataca aactctgcct atgatatcgg cgaatttgcc
     3001 gtgaatcaaa accttgcctt tgtttgacag tgctctcgga tacccgtcgc cgtagcctac
     3061 gggtacagtc gctatacgca tattgctctt tgacgtaaaa gtactgccgt agctgacggt
     3121 aaagcctggt cccacggatt ttatgtatgc tatatggctt ttcagctcta aagcaggatg
     3181 aagctcaagc tttgacttgt ctacttcctc cgacgggtaa agtccgtatg tcataatacc
     3241 gcttcttacc atatcaagga agttgtcgtc aaattctata attcccgcgg agttgcacat
     3301 atgctttatc ggtatctcaa ctccggcttt atcgagcttt ccgacaaaat caaggtactt
     3361 tttcttctga ttgttgcatg aagttttgtc
//
LOCUS       DBMDVO010000066         3366 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_12664_length_3366_cov_1.139357, whole genome shotgun sequence.
ACCESSION   DBMDVO010000066 DBMDVO010000000
VERSION     DBMDVO010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12664_length_3366_cov_1.139357"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="ACZPE2_01650"
     CDS             <1..1113
                     /locus_tag="ACZPE2_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1318583.1"
                     /translation="AFSEKLNSLGIDVYRHYAIDNYPTDVKLVVSDDGYGKNDFIKTE
                     KSLVVVTAPGPGSGKMAVCLSQLYHENKHGVKAGYAKFETFPIWNLPLKHPVNLAYEA
                     ATTDLNDVNMIDPFHLEAYGKTTVNYNRDIEVFPVLNTIFERIYGSSPYRSPTDMGVN
                     MAGFCITDDKVVCDAAKQEVIRRYYKALVREKNGEKVSGEIFKLDLLMKQLEVSVSDR
                     KCVEPAIEVSKKTNAPATAALLPDGQIVTGKTSSLLGATSAMLLNALKTIAGIDDSVK
                     LISPSVISPIQHLKTEHFGSKNPRLHTNEVLLALSISAATDENARKAMDALQYLRGCE
                     VHSTVILSSVDSDTLSKLGINVTCEPERETRNLFNR"
     gene            1214..>1510
                     /locus_tag="ACZPE2_01655"
     CDS             1214..>1510
                     /locus_tag="ACZPE2_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1318584.1"
                     /translation="MAAKYIFVTGGVVSGLGKGITAASLGRLLKDRGLKVTVMKLDPY
                     LNVDPGTMNPIQHGEVFVTDDGAETDLDLGHYERFIDESLTKNSSCTSGSVYFNV"
     assembly_gap    1511..1520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1521..2798
                     /locus_tag="ACZPE2_01660"
     CDS             <1521..2798
                     /locus_tag="ACZPE2_01660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1318585.1"
                     /translation="GQTFQIIPHITNEIKRRIALNETENTDIVMVEIGGTVGDIESLP
                     FLEAIRQFAQERAGRCMFIHVTLVPFLGASMELKTKPTQHSVKELLGIGIRPNVIVLR
                     TEHHIDRDIKEKIALFCNVPCENVIENIDLPTLYEIPLALEKEGLDKIVVNTLKLDCK
                     EPDHTDWMNMIDKNSHLSKEVNIALVGKYVELHDSYLSVNEALKHGGLDHDAHVNIDW
                     VDSESITEENVAERLKNADGILVPGGFGTRGIEGKIAAAKYARENKVPYLGICLGLQI
                     AIIEFARNVLGLKDANTTEIDPNTENPVIYLMPDQHNVTDMGGTMRLGAYPCMLDEGS
                     KAYKAYGKQLISERHRHRYEVNNDYRDLLEKNGMHICGLSPDKHIVEMVELKDHPWFV
                     ASQAHPELKSRPTKPHPLFSGLIGAALDYRESK"
     gene            complement(2864..>3366)
                     /locus_tag="ACZPE2_01665"
     CDS             complement(2864..>3366)
                     /locus_tag="ACZPE2_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1318586.1"
                     /translation="KNTKSDNTNLAILCSHNFRPPFDEPIPYAKKVGELVNMLADGHI
                     LVQRYGDILAGKRTWQEDLTRSNVRPTLPDAVAGDLTAAMPYRTLMNIIKFIEAVDKV
                     VPGFASEETLLYGPEIKFYSNKVKMDEKFNTNIEGLHCLGDSSGWTRGLMMASVMGVL
                     MGRELI"
BASE COUNT          975 a          839 c          765 g          777 t
ORIGIN      
        1 gctttttccg aaaagctgaa ctcactcggc attgacgttt atcgtcacta tgccattgac
       61 aactacccta ccgacgtaaa gctcgtagtc agcgatgacg gctacggcaa aaatgatttt
      121 atcaagaccg aaaaatcgct cgtcgtggta acggcgccgg gtccgggcag cgggaaaatg
      181 gctgtttgcc tgtctcagtt atatcatgag aacaagcacg gcgttaaggc gggatacgca
      241 aaattcgaga cttttccaat ttggaatctc ccgttaaagc accccgtcaa cctcgcatac
      301 gaggcggcaa cgaccgacct caacgacgtt aatatgatag acccgttcca tcttgaagcg
      361 tacggaaaaa ccaccgtaaa ttacaaccgc gacatagagg tattccctgt gctcaacact
      421 attttcgagc gtatttacgg aagctcgccc taccgctcac ccaccgatat gggcgttaat
      481 atggcaggct tttgtataac cgacgacaag gttgtatgcg acgccgcaaa gcaagaggtt
      541 ataagacgtt attataaagc cctcgtaaga gagaaaaacg gcgaaaaggt ttctggcgaa
      601 attttcaagc tcgacctttt gatgaagcag cttgaggtct cggtttcgga cagaaaatgc
      661 gtagagcctg caatagaagt gagcaaaaag actaacgctc cggcaacggc ggcattgctt
      721 cccgacgggc agattgtaac cggcaaaact tcatctcttc tcggcgcaac ctcggcaatg
      781 cttttaaacg cgttgaaaac aatagccggt atagatgatt cggtaaagct catctcgcca
      841 tccgtcataa gtcccataca gcaccttaaa acagagcatt tcggcagtaa aaatccgagg
      901 cttcatacga acgaggtgct tttggcgctt tcgatttccg ctgcaaccga cgaaaacgca
      961 cgaaaagcta tggacgcttt acaatatctt cgcggatgtg aggtccactc taccgtaata
     1021 ctctcttcgg tagactccga cacactctca aagctcggca taaacgtcac atgtgagccg
     1081 gagcgcgaga cgagaaatct tttcaacaga taaaacacgg tgctgcaccg ggcaggacgc
     1141 acggaatacc gcagcacagt ctttatatat actaaatcac gcgggaaacc gctctcataa
     1201 ggagagaaat tttatggcag caaagtatat tttcgtaaca ggcggcgttg tttccggtct
     1261 cggcaaggga attaccgcgg cttctctcgg cagactttta aaggacagag gtcttaaagt
     1321 caccgtaatg aagcttgacc cgtatctcaa tgttgacccc ggcacaatga atcccataca
     1381 acacggcgag gtatttgtaa cggatgacgg agcggaaacg gatcttgacc tcggacatta
     1441 tgagagattt attgacgaaa gcctcaccaa aaacagctca tgcacatccg gcagcgttta
     1501 ttttaacgtg nnnnnnnnnn gggcaaacct ttcagataat tcctcacata acaaacgaaa
     1561 tcaagagaag aatcgctctt aacgaaacag aaaataccga catagttatg gtggaaatcg
     1621 gaggtaccgt aggcgatatc gaaagtcttc cgttccttga ggccatacgt cagttcgctc
     1681 aggaaagagc cggcagatgt atgtttattc acgtgacgtt ggtacccttc ctcggggcat
     1741 caatggagct taaaacaaag cccacgcagc actcggtaaa agaactgctc ggcatcggca
     1801 taaggccgaa tgttatagtc ctccgcaccg agcatcacat agaccgcgac ataaaggaaa
     1861 agattgctct cttctgcaat gttccatgtg aaaacgtaat agaaaatatc gatctcccca
     1921 ctctttatga aattccgctt gcacttgaaa aagagggact tgacaaaata gttgtaaaca
     1981 ccttaaagct tgactgcaaa gagcccgacc acaccgattg gatgaatatg atagacaaaa
     2041 attcgcattt gtcaaaggag gtcaacattg cgctcgtcgg caaatatgtt gagcttcacg
     2101 actcatacct cagcgtaaac gaggcattaa agcacggcgg actcgaccac gacgcgcacg
     2161 tcaacatcga ttgggtcgat tccgaatcaa taaccgaaga aaatgttgcg gaacgtctta
     2221 aaaatgccga cggcatactt gttcccggcg gtttcggcac acgcggaata gagggcaaga
     2281 tcgccgcagc aaaatatgcg cgcgaaaaca aggttccgta tctcggcatt tgcctcggac
     2341 ttcagatagc aattatcgaa ttcgcgagaa acgtactcgg tctcaaggat gcaaacacaa
     2401 cggaaattga tcccaatacg gaaaaccccg taatttatct tatgcccgac cagcacaacg
     2461 taaccgatat gggcggcacc atgagactcg gcgcataccc gtgtatgctt gacgagggct
     2521 caaaagcata caaagcatac ggcaagcagc tcatttccga gcgtcaccgt caccgttacg
     2581 aggtaaacaa cgattacaga gacctgcttg agaaaaacgg tatgcacata tgcggactct
     2641 cccccgacaa gcatattgtt gaaatggtag agctcaagga tcacccgtgg tttgtcgcaa
     2701 gccaggcaca tcccgaattg aaatcgcgtc cgacaaaacc gcatcccctt ttcagcggac
     2761 ttataggcgc cgctctcgat tacagagaaa gcaagtaaga cttcaaaaag taaaggctgt
     2821 aaaaactctc gtttttacag ccttttttat tgcttttaaa tttttatata agctctctgc
     2881 ccataagcac gcccataacg cttgccatca taaggccgcg agtccaaccg cttgaatcgc
     2941 cgaggcaatg aagcccctca atattcgtat tgaacttctc atccatttta accttattgg
     3001 aatagaactt gatttcgggg ccgtaaagca gcgtttcctc gcttgcaaag ccgggtacca
     3061 ccttatctac agcctctatg aatttgatta tattcataag agttctgtac ggcattgcgg
     3121 ctgtaaggtc gcctgccacc gcgtccggaa gggtcggacg aacgttactg cgcgtcaaat
     3181 cctcctgcca tgtgcgctta cctgccaaaa tatcaccgta tctctgaacg aggatgtgac
     3241 cgtcggcaag catattcaca agttcgccga ctttctttgc atacggtata ggctcatcaa
     3301 acggcggacg gaaattgtgg ctgcaaagaa tagcgaggtt cgtattgtcg ctctttgtat
     3361 ttttat
//
LOCUS       DBMDVO010000067         3365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_12680_length_3365_cov_1.194240, whole genome shotgun sequence.
ACCESSION   DBMDVO010000067 DBMDVO010000000
VERSION     DBMDVO010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12680_length_3365_cov_1.194240"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2872)
                     /locus_tag="ACZPE2_01670"
     CDS             complement(<1..2872)
                     /locus_tag="ACZPE2_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1318587.1"
                     /translation="MQKTSTRILSVLLSVLMLLTLVPSALVSYAAGVSLNITDENGND
                     ITEIQQLTEYKTLQLKYTTTGELPEGTTVKWESTLPLLADVDDNGKVTAYDSSKTAII
                     QLWLDENVRVLPLVGDKMADEIMKQLEKSGINLDSMNDDLIVSIVRGIAGDTLADSLA
                     SALANMNVEITATIYDASGAKLASDKIKIKVQKSVIANIFPTGTHITNKKSVPLTVAV
                     GKTVQLYAAVTPVRLKHSVKWEMGGSVFDTSSGKRATISDTGLVTFTAAGSVTVRAYD
                     ASNVAFVDTITFNVVEQKDLPVESFDIISGTKTVTDSSVSVSEGSTTQLAITNVVPAG
                     AYTGDLTWEIADKSIAVVAASGTVTGLDGGDGLVEYSKSTTLTATAGGVSKNVTIKVS
                     RAGVVGSISSVEIEGNSAVPNDTPTTFKANVLPSRLNTNSSVKREWGLTNPDTKEIIW
                     ATAEAPAETKLATLSSDGVFTPKSSGISEIHVRATLNDKTVEDSLTVTAGKAITDFSI
                     SGSTAVNESKTTQLAIANIAPSDYDQALLGTVVWTSSDPSIATVDANGLVKGLDSGGT
                     AIWNTKKVTITATIGSVSKSVEITVRGNSIRHLTGVTITGNDYVIKDFPVTYTAKFSP
                     ERISVDKQRWGLTKDDGSAPWTSDWNIGDVNQKNAYASVDSNGVVTGISAGDTTLHAF
                     GRNGVTKVDGAYVETQKNITVVEVEPISIAFTAPTKTDYVEGSTELDLTGMEVKLTYN
                     RADIAEYYGEEAANAYTDDQLSVPVTDYTVSSVNTSILDSVQYIIVNVVRAGKTYHAI
                     FPITVHSKAVTSIELENPKKDYLEGDKTLDLSGLKVKANYSNAESEYVTDYTVDTSSF
                     DPELYDVEQNITVTYTHAGRSASATFPVIVYGKPVVSVDKGDYNGGWTSKDVTFTLSS
                     SHELDGVKYYFKTDSAGVEAPLEGNTLTVNVNSSDTYYFKAVN"
     gene            complement(3084..>3365)
                     /locus_tag="ACZPE2_01675"
     CDS             complement(3084..>3365)
                     /locus_tag="ACZPE2_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR1318588.1"
                     /translation="QHQNREVAMRMLKSKLLEIKEREHLDKISDIKGDQKEIGWGNQI
                     RSYVFMPYTLVKDNRTGYEMGNINAVMDGDIDGFINAYLKMQSQKKLEK"
BASE COUNT          822 a          807 c          738 g          998 t
ORIGIN      
        1 aatttacagc cttaaaataa tatgtatcgc tgctgttcac atttacggta agcgtgttgc
       61 cttccaatgg tgcttctacg cctgcggaat cggttttaaa gtaatactta actccgtcaa
      121 gctcgtgaga cgatgaaaga gtaaaggtta cgtccttgct tgtccatccg ccgttataat
      181 cgcctttatc aacgctgaca acgggtttac cgtatacaat tacggggaat gtagccgaag
      241 cgctgcgccc cgcatgtgtg tatgtaacgg ttatattctg ctctacatcg taaagctccg
      301 ggtcaaatga cgatgtgtca accgtgtaat cggtgacgta ttcggactcg gcgttgctgt
      361 aatttgcctt aaccttaaga cccgaaagat caagggtctt atcgccctcg agataatcct
      421 ttttaggatt ttcgagctca attgatgtta ccgccttaga gtgaacggtt atcgggaata
      481 ttgcgtgata tgttttgccg gctcttacta cgttaactat aatatactgc accgaatcaa
      541 gtatcgaagt gttgacggag cttaccgtat aatccgttac cggaacggaa agctgatcgt
      601 cggtataggc gtttgccgcc tcttctccgt aatattcggc aatatccgca cgattatatg
      661 taagcttaac ctccataccg gtgagatcga gctctgtgct accctctaca taatcggtct
      721 tagtgggcgc ggtaaacgca atacttatcg gctcaacctc aactaccgtt atattctttt
      781 gagtttctac gtatgcgccg tcaacctttg taacgccgtt cctgccgaat gcatgtagtg
      841 ttgtatcgcc tgccgaaata cccgtgacaa caccgtttga atcgaccgaa gcatacgcat
      901 tcttctgatt tacgtcgccg atattccagt cggaggtcca gggagccgag ccgtcgtcct
      961 tagtgagacc ccaacgctgc ttatcgaccg aaattctctc gggagagaat ttagcggtgt
     1021 aggtaaccgg aaaatccttg attacataat catttcccgt tatggtaact cccgtgaggt
     1081 gtcttattga gttaccgcgt accgttatct ctacggattt agacacgctt ccgatggttg
     1141 cggttattgt gacctttttg gtgttccaaa tggccgttcc gccggaatca agacctttta
     1201 caagaccgtt tgcatcaaca gtagctattg acgggtcgga agaagtccat acgacagttc
     1261 cgagaagtgc ttggtcgtaa tcggacggtg ctatattcgc gattgcaagc tgcgtggtct
     1321 tgctctcatt aaccgctgtg ctgccgctga tgctgaagtc tgttatcgcc ttgcccgccg
     1381 ttacggtaag agagtcctca acggttttat cattaagagt tgctcttacg tgtatttcgc
     1441 taataccgct cgatttcggg gtgaatacac cgtcggacga aagcgtagca agctttgtct
     1501 cggcgggtgc ctcggcggtt gcccatatta tttcttttgt atcgggattt gtaagtcccc
     1561 actctcgttt taccgacgag tttgtgttaa gtcttgacgg taacacattt gccttaaagg
     1621 tagtcggcgt atcattggga acggcactgt taccctcaat ttcaacagag gagatactgc
     1681 cgaccacacc ggctcttgac acctttattg taacattttt ggaaacgccg cccgccgttg
     1741 cggtaagggt tgtggattta ctgtattcaa cgagtccgtc gccgccgtca aggccggtta
     1801 ccgtaccgga ggcagcaacc acggcaatcg atttatcggc gatttcccaa gtaaggtcgc
     1861 cggtatatgc tcctgcggga acaacatttg taattgcaag ctgcgttgtg gagccttcac
     1921 ttacggatac ggatgaatct gttacggttt ttgtgccgct tataatatca aaggattcaa
     1981 ccgggagatc cttctgttca actacattaa aggttatggt atcgacaaaa gctacattgc
     2041 tcgcgtcgta tgccctaacc gttaccgagc ctgctgcagt aaatgtgaca agtcccgtat
     2101 cggaaatcgt tgcacgcttg ccggagcttg tatcaaatac gctgccgccc atctcccatt
     2161 ttacagagtg cttaagtctt accggtgtta ccgccgcgta aagctgaacc gttttgccca
     2221 cggcaacggt aagaggaacg ctctttttat tggttatatg agttccggta ggaaagatgt
     2281 tcgcaataac gctcttttga acctttattt taattttgtc acttgcaagc tttgctccgc
     2341 ttgcgtcata tatagtagcc gttatttcga cattcatatt ggcaagagca gacgcaaggc
     2401 tgtccgcaag agtatcacct gcaatgcctc tgacaataga aactatcagg tcatcgttca
     2461 tactgtcaag attaataccc gacttttcaa gctgtttcat aatctcgtca gccattttat
     2521 cgcctacgag aggaagcacg cgcacgtttt catcaagcca aagctgaatt atagctgtct
     2581 ttgacgaatc atatgccgta accttgccgt tatcgtctac atcggcaaga agcggtaggg
     2641 tactctccca ctttaccgta gtaccctcag gcaattcgcc tgtcgtggta tactttagtt
     2701 gaagcgtttt atactcggtc aactgctgta tttcggtaat gtcattaccg ttttcgtccg
     2761 taatgttaag cgagactcct gccgcgtagc tcacaagagc agacggaacc aaagtcaaca
     2821 gcataaggac agacagcaaa acgctcaaaa tcctggttga tgttttttgc atacagatct
     2881 tcctttctct gaaaaaccgt ttttacatac aaataataac aaattctaca tattttttca
     2941 atgcacacat ttttacaaat agtattataa gccggtctgt gttttagtga ttatacaata
     3001 cgaacaaaca aaaaagcgcg gaattacaca ctccgcgctt taataacata ttgatatacc
     3061 gccttaccgc actgataaag cgtttatttc tcaagctttt tctgagactg cattttgaga
     3121 tacgcattta tgaaaccgtc aatatcgccg tccataaccg cattgatgtt acccatttcg
     3181 tagcccgtgc ggttatcctt gacaagtgtg tacggcataa atacatacga acgtatctga
     3241 ttgccccagc ctatctcttt ttggtcgccc ttaatatccg aaattttgtc aagatgctcg
     3301 cgctccttga tttcaagaag cttggatttt aacattctca tggcaacctc tcggttctga
     3361 tgctg
//
LOCUS       DBMDVO010000068         3297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13090_length_3297_cov_1.173967, whole genome shotgun sequence.
ACCESSION   DBMDVO010000068 DBMDVO010000000
VERSION     DBMDVO010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13090_length_3297_cov_1.173967"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="ACZPE2_01680"
     CDS             <1..519
                     /locus_tag="ACZPE2_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1318589.1"
                     /translation="LMSLYSVVFQDVTLFDNTITENIRIGKKDATDEEVIAAAKLANV
                     TEFVEKLPDKWNSTIGENGCELSGGERQRISIARAFLKDAPIILLDEATASLDVENET
                     AIQTALSRLIKNKTVLIIAHRMRTVSGADKIVVLKDGSVAEQGSPATLIKENGEFARM
                     VKLQTESLEWTI"
     assembly_gap    700..709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(845..1939)
                     /gene="ychF"
                     /locus_tag="ACZPE2_01685"
     CDS             complement(845..1939)
                     /gene="ychF"
                     /locus_tag="ACZPE2_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1318590.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITNAGAEVANYAFCTIEPNVGVVAV
                     PDYRLDELAKMYSPKKITPAVIEFVDIAGLVKGASKGEGLGNKFLSHIREVDAVIHVV
                     RCFDNDDIMHVSGSVDPKRDIETINLELILSDLEILDRRIDRTAKAAKGDKSLLTELE
                     FLKRLKTELENGVSARAVEADDDEKAILADIGLLSAKPVIYACNMSEDDFASNIENNE
                     RYKAVCEIAKAEGAEVLPICAELEAEISSLSKEEKEMFLSDAGIESGGLDMLIQRSYS
                     LLGLISYLTAGEPEVRAWTIKKGTKAPQAAGKIHSDFERGFIRAEVISFDELMACGSM
                     QAAKEKGKIRSEGKDYVMQDGDIVLFRFNV"
     gene            complement(1956..2483)
                     /locus_tag="ACZPE2_01690"
     CDS             complement(1956..2483)
                     /locus_tag="ACZPE2_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025628.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1318591.1"
                     /translation="MSQGSKKKKGIALALVLLLVMSALAVVVFGKGYIRNKNRGRSPH
                     VISASSAEQIAERIISETSLAGIVKLQNEQVGNYYGVPVAIIENSAVYKSSSALSADE
                     IAVFRVSSEAEKKIAASAVDGRLSDCRSSYSTLSSDENRKIDNCLVEVSGDYVIMVIC
                     GDPGKASEAISEFYK"
     gene            complement(2714..>3297)
                     /gene="prmA"
                     /locus_tag="ACZPE2_01695"
     CDS             complement(2714..>3297)
                     /gene="prmA"
                     /locus_tag="ACZPE2_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1318592.1"
                     /translation="GDKLLIRPLWIDDYDNSDNRAVLSIEPGLAFGTGGHNTTKLCLE
                     ALEKYVKKGSSVLDTGCGSGILSIASLLFGAEKAVGVDIDELAVKTARENGRVNGFTE
                     PEFTVICGNLADKVTGKFDVVAANIVADVVMMFSSQVGAFMKDDAVFITSGIIAPRAD
                     EVKAKLCENGFNIIETRRDGDWYCFVCNRRCGE"
BASE COUNT          906 a          841 c          673 g          867 t
ORIGIN      
        1 cttatgtcgc tttattcggt agttttccaa gatgtaacgc tctttgacaa cacaataacg
       61 gaaaacatac gcataggcaa aaaagacgcc accgacgaag aagttattgc cgccgcaaaa
      121 cttgcaaacg taaccgaatt cgttgaaaag cttcccgaca aatggaattc aaccataggc
      181 gaaaacggat gcgaattatc cggaggcgaa cgtcagagaa tatcaatcgc aagagcattt
      241 ttgaaggacg cccccataat tttgcttgac gaagccacgg caagccttga cgttgaaaac
      301 gagacggcaa tacaaaccgc tctttcacgc cttattaaaa ataagaccgt ccttattatt
      361 gcacatcgta tgcgcacggt atctggcgcc gacaaaatcg ttgtgctgaa agacggctcg
      421 gtagcggaac agggttctcc cgcaacgctt ataaaagaaa acggcgaatt cgcccgtatg
      481 gtcaagcttc agacggaaag tcttgaatgg actatataac attcggtatt atcgaagctt
      541 aacaaataaa aacggcaggt actgcggtgc ctgtcgtttt tattttcggt atcgcacata
      601 agtcagcatg cacataatat ttaatccgcc gtgcggtaaa aattttcacg gaagttaata
      661 aaaaaagaaa gagccgacac agtaatgatt ccgtcataan nnnnnnnnnt gactggattc
      721 gaaccagccg ccttcagagt cggagtctga cgctctatcc aaatgagcta caggcgcata
      781 ttaaataaaa cacaactgct ttgtgcggac gaagcatctc gaccgcacaa agcattttta
      841 tatctcagac gttaaatctg aaaagaacta tatctccgtc ctgcataaca tagtccttgc
      901 cctcggagcg tattttgccc ttttcctttg cagcctgcat cgaaccgcaa gccataagct
      961 cgtcaaaaga gataacttcg gcacgaataa agccacgctc aaaatcggag tgtattttac
     1021 ctgccgcctg gggagccttg gtacctttct ttattgtcca cgctctgacc tcgggttcgc
     1081 ctgcggtgag ataagatatg agtccgagca agctgtacga acgctgtata agcatatcga
     1141 gtccgccgct ctcaatgccg gcgtccgaca ggaacatttc cttttcctct tttgagagag
     1201 acgaaatttc cgcttcaagc tcggcacata tcggaagcac ttcggcaccc tctgcctttg
     1261 ctatttcaca gacggcttta tatctctcgt tattttcgat gtttgaggca aaatcatctt
     1321 cgctcatatt gcaggcgtaa attacgggct ttgcggaaag caaacctata tccgccaata
     1381 tggccttttc atcgtcgtcc gcctccacgg cacgggcact gacgccgttt tcgagctccg
     1441 ttttaagacg ttttaaaaac tcgagttccg taagaaggct cttatcgccc ttagccgcct
     1501 tagcggtacg gtctatgcgg cggtcaagaa tctcaaggtc ggagagaatg agctcgagat
     1561 ttatggtttc aatgtctctt ttcgggtcaa ccgagccgct gacgtgcatt atgtcgtcgt
     1621 tatcaaagca tcgtacaaca tgtataacag cgtcaacctc gcggatatgc gacagaaact
     1681 tattaccgag accctcaccc ttggaggcac cttttacaag acccgctata tcgacaaatt
     1741 cgattaccgc gggagttatt tttttaggcg aatacatttt ggcaagctcg tcaagcctgt
     1801 aatcgggcac agccacaacg cctacattcg gctcaatagt gcagaacgca tagtttgcaa
     1861 cctcggcgcc tgcattggtt atagcattaa aaagcgtact cttgcctacg ttcggcaaac
     1921 ctacgatacc aagtttcatt atgaatttac atcctttatt tataaaattc cgatattgct
     1981 tcggacgcct taccggggtc accgcaaata accataataa catagtcacc gcttacctcg
     2041 acaagacaat tatctatttt tctgttttcg tcggagctga gtgtggaata agagctgcga
     2101 caatccgaaa gtctgccgtc aacagccgac gccgcaatct ttttttcggc ctcgctactc
     2161 accctgaaca ccgcgatttc atccgctgac agcgccgatg agcttttgta taccgcggaa
     2221 ttctcgataa ttgcgacagg cacaccatag taattgccga cttgctcatt ttgaagcttc
     2281 acaatgccgg cgagagatgt ttccgatata atccgctccg ctatctgctc cgccgatgac
     2341 gccgaaataa catgcggcga tctgcccctg ttcttattac gtatatagcc tttgccgaaa
     2401 acaacgacag caagagccga catcacgagc agcagtacta atgcaagtgc tatccccttc
     2461 ttctttttgc tcccttgtga cataagaaaa ttccttttcc gtacataacg gtggattttt
     2521 aataagctac cccaaataca gtttaaagcc gcaagccgca aaacaactta taaatattat
     2581 cacaatttgt gatatttagc aacagcacaa ttttcctttt cggttttttg atgtcatttg
     2641 ccgcaatgca aaggtatttt ctccttgaaa cacgcaaaac ggcggaaaaa ccgccgtttg
     2701 attttacatg gatttattca ccgcagcgtc tgttacatac aaaacaatac caatcgccgt
     2761 cacggcgtgt ttcgattatg ttaaacccgt tttcacagag ctttgctttt acttcgtccg
     2821 cgcgcggtgc tataatgccg gaagtaataa aaacggcgtc gtccttcata aatgcaccca
     2881 cctgtgatga aaacatcata acgacatccg ccacgatatt tgccgcgaca acgtcaaatt
     2941 tacccgttac cttatctgca agattgccgc atataaccgt aaattcgggc tcggtaaagc
     3001 cgtttaccct gccgttttca cgagcggttt taacagccag ctcatctata tcaacaccga
     3061 ccgctttttc cgctccgaaa agaagcgacg caatggacaa tataccgctt ccgcaacccg
     3121 tatcaagaac ggacgagcct ttttttacat atttttcaag agcttcaagg catagcttgg
     3181 tggtattatg tccgcccgta ccgaatgcaa gccccggctc tatagagagc acggcacgat
     3241 tgtcggaatt atcgtaatca tcaatccaca gcggtcttat aagaagctta tcgccta
//
LOCUS       DBMDVO010000069         3293 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13112_length_3293_cov_1.047306, whole genome shotgun sequence.
ACCESSION   DBMDVO010000069 DBMDVO010000000
VERSION     DBMDVO010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_3293_cov_1.047306"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="ACZPE2_01700"
     CDS             <1..1745
                     /locus_tag="ACZPE2_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1318593.1"
                     /translation="LPFDSGTRTVKSSLEIGYLKQNEALNSENTLREEIEDALSEVYD
                     VREKLLEASKRISASTPGSEEYRNLSEAYEKLTNQYDALDGYTADTRINIVLNGLGFG
                     DFNLEQKTAHLSGGEKIRFGIAKILLHNPELLILDEPTNHLDFTMLTWLEDYLSSYKG
                     SVLIVSHDRYFLDKVADNICEIENGSLYKYKGGYSAFLTQKEERIRHAEKEYEKQQSE
                     LAAMRDYVAKNLAKSSSSNSVGSRVKALEKMELQAKPNPKQKEVHFKFEYDFEPHKVV
                     LSCKDVGIYVGNAATGKQLYENISLEVLKGEKIAIIGKNGVGKSSFLKAVLKKIPYTG
                     QIKFGGNVKVAYFDQELGDLNLDDTVIEAVHRAYPTKTEFEIRSALGRLLIEDEAVFK
                     RVRDLSGANRAKVAFCIIMFKRANVLIFDEPTNHLDYIAKEALDDALKEFTGTLITVS
                     HDRYFLNCVPDKIIEMFPTGFNEYNGGYDYYLEHKKPEIVEKTVKADSENKAAYKENK
                     KNKADDRKRRAKLLSLQREMDSLEAEIATLKAESEKPDVSSDYQKLSDILALIADKES
                     CLEDAETEWLELSE"
     gene            complement(1813..>2201)
                     /locus_tag="ACZPE2_01705"
     CDS             complement(1813..>2201)
                     /locus_tag="ACZPE2_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DinB/UmuC family translesion DNA polymerase"
                     /protein_id="HIR1318594.1"
                     /translation="LLAGGVQIGIKNTALFTSQSQSKLIYPTQNSREIALKAFSLFKQ
                     IYKWQTPVRAVTVRAIELLNPDKPQQLSLGFDMTRHEKLERLDKAMLRINEKYGKGTV
                     VEATVLNGTKMPTTVPSADKDISFLP"
     assembly_gap    2202..2211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2212..2948)
                     /locus_tag="ACZPE2_01710"
     CDS             complement(<2212..2948)
                     /locus_tag="ACZPE2_01710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1318595.1"
                     /translation="MERAILHCDLNNFYASVETVVSPELRGKAIAICGRTEDRHGIVL
                     AKSEAAKKCGVKTGDVIWQAKGKCPNLLVFPPRFSEYIKYSRAVKSIYGRYTDMIEPF
                     GIDECWLDVSGSTRLFGSPDTIADDVREAVKKELGLTISVGVSFNKVFAKLGSDMKKP
                     DAVTVIRRENFRDMIWQLPAEDMLWVGRSTSKVLKKYGIITIGDIAKSDPAFLKTTFG
                     KNGVVLWRCANGLENSPVCNMGYRPPV"
     gene            complement(3099..>3293)
                     /locus_tag="ACZPE2_01715"
     CDS             complement(3099..>3293)
                     /locus_tag="ACZPE2_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR1318596.1"
                     /translation="FYFRTTDVTGTITLPEGIEMCMPGDNVVMSVELITPIAIEQGLR
                     FAIREGGRTVGSGVVTEIDE"
BASE COUNT          987 a          750 c          720 g          826 t
ORIGIN      
        1 tactcccctt tgacagcggt acgagaaccg tcaaaagctc gcttgaaata ggatacctca
       61 agcaaaacga agctttgaac tccgaaaata ctctgagaga ggaaatcgag gacgcactct
      121 cggaggtcta tgacgtaaga gaaaaactgc ttgaagcatc aaaacgaatt tcagcctcaa
      181 cacccggctc cgaggaatac agaaatcttt cggaagctta cgaaaaactg acaaaccaat
      241 atgacgctct tgacggctac actgcagaca cgagaataaa catagtttta aacgggctcg
      301 ggttcggcga ttttaacctc gaacaaaaga cggctcacct gagcggcggt gaaaaaatac
      361 ggttcggcat tgccaaaata ctgcttcaca atccggaatt gctcattttg gacgagccta
      421 caaaccattt ggactttacc atgctgactt ggcttgaaga ttatctctct tcatacaagg
      481 gctcggtact gatagtatca catgacagat attttctcga caaggtagct gataatatat
      541 gcgaaatcga aaacggaagt ctgtataaat ataagggcgg ctacagcgca tttttaactc
      601 agaaggaaga gagaatacgc cacgccgaaa aggaatatga aaagcaacaa tccgagcttg
      661 ccgcaatgcg cgattatgtc gcaaaaaatc tcgctaaatc ttcaagcagc aacagcgtag
      721 gctcacgagt taaggctctt gaaaaaatgg agcttcaggc aaagcccaac ccaaagcaaa
      781 aggaagtcca tttcaaattt gaatatgatt ttgagcctca caaggtggtg ctctcttgca
      841 aggatgtcgg aatctatgtc ggaaacgccg caaccggcaa gcagctttat gaaaacatct
      901 ctcttgaggt tttaaaaggc gagaaaatcg cgattatagg caaaaacggc gtcggcaaat
      961 catctttttt aaaggctgtt cttaaaaaaa taccatatac ggggcagatc aaattcggag
     1021 gaaacgtaaa ggtcgcttat tttgaccagg agctcggcga cctcaacctt gacgacaccg
     1081 taatagaggc tgtgcacaga gcttatccga caaagactga atttgaaata agaagtgcgc
     1141 tcggcaggct gctgattgag gatgaggctg tatttaaacg ggtacgcgat ctgtcgggag
     1201 caaaccgtgc caaggttgca ttttgcatta taatgtttaa gcgcgccaat gtgctgattt
     1261 tcgacgagcc taccaaccac cttgactaca ttgccaaaga ggctcttgac gacgctctga
     1321 aggaatttac gggtacgctg ataacggtat ctcacgacag gtacttttta aactgcgtac
     1381 cggataaaat tatcgagatg ttcccgacgg gatttaatga atacaacggc ggatacgact
     1441 attaccttga gcataaaaag ccggaaatcg tcgaaaaaac cgttaaagcg gacagcgaga
     1501 ataaagccgc atacaaagag aacaaaaaga acaaggcgga cgacagaaaa cgcagagcta
     1561 agctgttatc tcttcagcgc gaaatggaca gtcttgaagc tgaaattgca acgcttaaag
     1621 cggagtcgga aaagcccgac gtctcgagcg attatcaaaa attatcggat attctcgcgt
     1681 tgattgctga caaggagagc tgccttgagg atgctgaaac cgagtggctt gagctttcgg
     1741 aataaactta aaagcgactg taaaggctga gtagcactca accgtttaca gccgcttttt
     1801 tatgttatct ctctaaggca aaaacgatat atccttatcg gctgacggta ctgttgtcgg
     1861 catttttgtg ccgtttaaca cagttgcctc cacaacggta cccttgccgt atttctcgtt
     1921 tattcgaagc attgctttat cgagtctttc gagcttttca tgacgcgtca tatcaaagcc
     1981 gaggctcagc tgctgaggct tatccggatt taaaagctct attgcgcgca ccgtgacagc
     2041 tcttaccgga gtttgccatt tatatatctg cttaaacagc gaaaaggctt taagggcaat
     2101 ttctcggctg ttttgtgttg gataaatgag cttactctgc gattgtgacg tgaacaatgc
     2161 ggtgttttta attcctatct gcacaccgcc ggcaagaagg cnnnnnnnnn nttgaccgga
     2221 ggtctgtaac ccatattaca gacgggcgaa ttttcaaggc cgttggcgca gcgccacaac
     2281 acaacgccgt tcttaccgaa cgtagtcttt aagaacgcag gatcactttt tgcaatgtcc
     2341 cctattgtta taataccgta ctttttaagc accttagagg tgcttcttcc gacccatagc
     2401 atatcctcgg ctggtagctg ccaaatcata tcgcgaaaat tttcacgtcg tataaccgta
     2461 accgcatcgg gctttttcat atcgctaccg agctttgcaa acactttatt gaatgacaca
     2521 cccacggaaa tagtaagtcc gagttctttt tttacagcct ctctgacatc atcggcgatg
     2581 gtatcgggag agccgaatag ccgtgtactt ccgcttacat caagccagca ctcgtcaata
     2641 ccgaacggct ctatcatatc ggtgtatctg ccgtatattg attttaccgc acgtgaatat
     2701 ttgatatatt cggaaaatcg aggaggaaaa accaaaagat taggacattt gccctttgcc
     2761 tgccatataa cgtcgcctgt ttttaccccg catttttttg ccgcctcgga ctttgccagc
     2821 actatgccgt gcctatcctc ggttctgccg caaattgcaa tagctttgcc gcgcagttcc
     2881 ggagaaacaa ccgtttcaac cgaagcgtaa aaattattca aatcacaatg aagtattgcc
     2941 cgttccataa aatcaccgaa aaaattattg taccgtcatt gtaaccgaac atttgttctg
     3001 tgtcaatagt aaaaatataa cagtcccgaa attcggtctg aacataaaaa aatcacccct
     3061 gaaaaacaga ggtgattttt aattatcgta ttaacgaatt attcgtcaat ttcagtaaca
     3121 acgcctgaac caacggtacg tccgccttca cgaatagcga aacgaagacc ctgctcaata
     3181 gcgataggag tgataagctc aacgctcata acaacgttat cgccgggcat gcacatctca
     3241 attccctcgg gaagagtgat agtaccggta acgtcggtag ttctgaaata gaa
//
LOCUS       DBMDVO010000070         3293 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13113_length_3293_cov_1.022556, whole genome shotgun sequence.
ACCESSION   DBMDVO010000070 DBMDVO010000000
VERSION     DBMDVO010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13113_length_3293_cov_1.022556"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="ACZPE2_01720"
     CDS             <1..772
                     /locus_tag="ACZPE2_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1318597.1"
                     /translation="ASIGARPVTARVNTLKASPDELIALLSGENAVAEKCPEDENYII
                     LKNTGAVEELKAYKAGFFHVQDISCGKAVKALSPKAGDTVFDMCSSPGGKAFTAAQLM
                     KNKGKILAFDLYPQRVKLCEEGALRLGIDIIEAKVGDACVFLPEYEKAADKVLCDVPC
                     SGFGIIGRKPEIRYKDIAIIDNLLPVQYNILTNAAKYVKSGGTLVYSTCTLSRAENED
                     VCEKFLGNNAEFRKISFNTIIPTENGGDGFFFAVFGRI"
     gene            772..1815
                     /gene="rlmN"
                     /locus_tag="ACZPE2_01725"
     CDS             772..1815
                     /gene="rlmN"
                     /locus_tag="ACZPE2_01725"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1318598.1"
                     /translation="MLSKTDIRSLSLSELTEKTVSMGLPKYRAGQIYSWLNEKGAVSF
                     DDMTNIGKQLRKTLDDEFYICNCKIDTKLVSQKDDTVKYLFDLSDGEYVESVVMKYKY
                     GYTICISAQLGCKMGCSFCASAIGGFKRHLRPSEMLSEVITAQNDLGIRISHIVLMGT
                     GEPLDNYDNVMKFLSLVTDENGLNISMRHISLSTCGIVPRIYDLAEKRLGLTLSVSLH
                     APNDSIRSRSMPVNLKWNIEELLKACRYYTEVTSRRISFEYAMISGLNDSDECARELS
                     SRLRGMLCHVNLIPVNNVRENNYVRSDRERLRSFSEILQKNGINVTVRRTLGSDINAS
                     CGQLRAKKITDKN"
     gene            1839..2567
                     /locus_tag="ACZPE2_01730"
     CDS             1839..2567
                     /locus_tag="ACZPE2_01730"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1318599.1"
                     /translation="MKIVAKTDVGMVRESNQDSYTTGELPNGVAWAVVCDGMGGAAGG
                     NVASSTAVKVIAEQITSSFRPGMSANSIKNMMVSAINAANISVYDISCSNSELYGMGT
                     TVVCAIVMEHIAYIAHAGDSRAYIVSHGALTQLTRDHSVVQELVEHGKITALEAKRHP
                     NKNIITRAVGVSREILVDFCEQEMKDGDVLLLCTDGLTNFVESDEMCDLTKDDMFYEF
                     AERLVDRANKNGGGDNVTVVAVTY"
     gene            2583..>3293
                     /locus_tag="ACZPE2_01735"
     CDS             2583..>3293
                     /locus_tag="ACZPE2_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1318600.1"
                     /translation="MENYCGKRLDGRYEIREIIGVGGMAVVYKAYDVIDDRIVAIKIL
                     KEEFLANDEFRRRFKNESKAIAVLSHPNIVKVYDVSFGDRLQYIVMEYIEGITLKEYI
                     EQQKVINWKEAVHFVTQILKALQHAHDKGIVHRDVKPQNIMLLQDGTIKVTDFGIARF
                     CRGDTRTMTENAIGSVHYISPEQARGEITDDKADIYSVGVVMYEMLTGQLPFQSDSAV
                     SVAIMQLQQEAKRPREINP"
BASE COUNT          946 a          682 c          838 g          827 t
ORIGIN      
        1 ggcgtccata ggcgcaagac ccgtaactgc gagagtgaat actcttaaag cttcaccgga
       61 tgagcttata gccttgcttt ccggcgaaaa tgcggttgcc gaaaagtgcc ccgaggacga
      121 aaattacata atacttaaaa ataccggtgc ggtagaagag ctcaaagcgt ataaagcggg
      181 atttttccat gttcaggaca tctcctgcgg caaggcggta aaagcactgt cgccgaaagc
      241 cggcgatacc gtttttgata tgtgctcttc acccggcgga aaggcgttta cggcggcgca
      301 gcttatgaaa aacaagggca aaatactcgc ttttgacctt taccctcagc gcgtaaagct
      361 ctgtgaagag ggagctttaa ggctcggtat agatataatc gaggctaagg tgggcgacgc
      421 ctgtgtattt ttgcccgagt atgaaaaagc ggcggacaag gtgctctgtg acgtgccctg
      481 ctcaggcttc ggaataatcg gcaggaagcc cgaaatacgc tacaaggata ttgcgattat
      541 tgacaattta ctgcctgtgc agtataatat tttgacaaat gcagcaaaat atgtaaagag
      601 cggcggcacg ctagtttatt cgacgtgtac gctgagcaga gccgaaaatg aagacgtttg
      661 cgagaaattt ctcggtaata acgcggagtt ccgcaaaata tcttttaata cgattattcc
      721 gaccgaaaac ggcggtgacg gcttcttctt tgcagtattc gggagaattt gattttgagt
      781 aaaactgata tacgttccct gtctttgtcg gagcttacgg aaaagacagt ttcaatgggg
      841 cttccgaaat accgtgcagg gcagatttat tcctggctca acgaaaaagg cgcggtatct
      901 tttgacgata tgacaaatat cggcaagcag ctccgaaaaa cacttgatga cgagttttat
      961 atctgtaact gtaaaataga tactaagctt gtctcgcaaa aagacgatac ggtaaaatat
     1021 ctttttgatt tatccgacgg cgaatatgtt gagagcgtcg ttatgaaata caaatacggc
     1081 tataccatat gtatctcagc tcagctcggc tgtaaaatgg gctgttcgtt ctgtgcttcc
     1141 gcaataggcg gttttaagcg tcatttgcgt ccctcggaaa tgctgtcgga ggtaataacg
     1201 gctcagaacg acctcggcat acgcatttcc catatagtgc ttatgggtac cggagagccg
     1261 cttgacaatt acgataacgt tatgaagttt ttgtcgcttg taaccgatga aaacggtctt
     1321 aatataagta tgcgccacat atcgctttcc acttgcggta tagttccgcg catatatgat
     1381 ttggcagaaa aaagacttgg gcttactctg tcggtttcgc ttcacgcacc gaacgacagt
     1441 atccgttcgc gctctatgcc ggtaaactta aagtggaata tcgaggagct tttaaaggcg
     1501 tgcaggtatt ataccgaagt aacttctcgc agaatatcat ttgaatatgc catgataagc
     1561 ggccttaacg attcggacga atgtgcgagg gaactttcct caagacttcg cggaatgctg
     1621 tgccacgtga atttaattcc cgttaacaac gtcagggaaa acaattacgt aagaagcgat
     1681 agagaaaggc tcaggagctt ttccgaaata ctgcaaaaaa acggtataaa cgttacggtt
     1741 cgccgaacgc tcggaagtga tattaacgcc tcgtgcggac aacttagagc taaaaagata
     1801 accgataaaa attgattgtg aaaaagaggt gatctgctgt gaaaatagtc gcaaaaaccg
     1861 atgtgggcat ggttcgtgag agcaatcagg attcatacac caccggtgag ctgccgaacg
     1921 gtgtcgcatg ggccgtggtc tgcgacggta tgggcggtgc tgccggagga aacgtggcgt
     1981 catccacagc ggtaaaagta atagccgagc agattacatc ttcgttcaga cccggtatga
     2041 gtgcaaattc catcaaaaat atgatggtgt ctgccataaa tgccgccaac atcagcgtat
     2101 atgacataag ctgttcaaat tcggagcttt acggtatggg tactaccgtg gtgtgtgcca
     2161 ttgttatgga gcatattgca tatattgccc acgccggaga cagcagagcg tacattgttt
     2221 cgcacggagc tttaacccag ctcacgcgtg accattcggt tgtgcaggag ctcgtggaac
     2281 acggcaaaat aaccgctctt gaggcaaaaa ggcatccgaa taagaatata ataacgcgcg
     2341 ccgtcggcgt gagccgcgaa attcttgtgg atttttgcga gcaggaaatg aaagacggcg
     2401 atgtgctttt actttgcacg gacggtctta caaatttcgt cgagtccgat gaaatgtgcg
     2461 atcttactaa agacgatatg ttctatgagt ttgccgagcg actggtcgat agggcaaaca
     2521 aaaacggagg cggcgacaat gtcactgtcg tagccgttac ctattaaaag gagtgatcat
     2581 ttttggaaaa ttactgcggt aaaagacttg acggcagata tgaaatcaga gaaatcatag
     2641 gtgtgggcgg tatggctgtt gtctacaagg cttatgatgt gatcgacgac agaatagttg
     2701 cgatcaaaat tctcaaagag gaatttcttg caaatgatga attccgccgt cgcttcaaaa
     2761 acgaatcaaa ggctattgcc gttttgtcgc acccgaatat agttaaggtg tatgacgtga
     2821 gcttcggcga cagacttcag tatatagtta tggaatatat tgagggcata actctcaaag
     2881 aatatataga gcagcaaaag gttataaatt ggaaagaggc tgttcacttt gtaacgcaga
     2941 ttttaaaagc gcttcagcac gcgcatgata agggcattgt acacagagac gtaaagccgc
     3001 aaaatattat gctattgcag gacggcacca ttaaagtcac cgatttcggc atagcgcgtt
     3061 tctgtcgcgg cgatacacgc actatgaccg aaaatgccat cggctcggtt cactatatca
     3121 gtccagagca ggcgagaggc gaaataaccg acgacaaggc cgatatttat tccgtaggtg
     3181 tggttatgta tgaaatgctt acgggtcagc ttccgttcca gtcggacagt gctgtttccg
     3241 tagcgataat gcagcttcag caggaggcta aacgtccgcg agagataaat ccc
//
LOCUS       DBMDVO010000071         3289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13132_length_3289_cov_1.316813, whole genome shotgun sequence.
ACCESSION   DBMDVO010000071 DBMDVO010000000
VERSION     DBMDVO010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13132_length_3289_cov_1.316813"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(258..839)
                     /gene="yfbR"
                     /locus_tag="ACZPE2_01740"
     CDS             complement(258..839)
                     /gene="yfbR"
                     /locus_tag="ACZPE2_01740"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HIR1318601.1"
                     /translation="MKGKFFAFTSRLKYINRWALMRNTTYENLSQHSGEVATIAHALA
                     VIGNKRLGKEYNAERAALLGLYHDTPEIITGDMPTPVKYYSREMRSVFGRVEKEACQR
                     LLNMLPDDLSEEFSPLFFKEERDAELWRLIKAADKLSAYIKCIEERKAGNCEFEDAER
                     TIRKALDESECREAHIFLEEFIPAYELPLDKLK"
     gene            complement(905..1891)
                     /locus_tag="ACZPE2_01745"
     CDS             complement(905..1891)
                     /locus_tag="ACZPE2_01745"
                     /inference="COORDINATES: protein motif:HMM:NF026101.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4474 domain-containing protein"
                     /protein_id="HIR1318602.1"
                     /translation="MKSMSVRITSAVIALIMIACVFSSCKSKKGGDEEITRSNQVIAG
                     PVTTAENGEATTGEETTGGETTAAATAAAAKASSSLNVPSVTTPDFNNTTSASGNSAA
                     SSMGSLGAAVLGALANADPETLAAFVNNMGFGYDAEQGIFYSLMDSWQRDANFISQYD
                     KYAAIFGNMRYLTTTVDFYYDGLDWRIQLWKGQYGPFGGGEVGVYTKDPETNDKLYNC
                     ADNDHLLNMYYDLYLTPNTYAVKNRFFYRDAWTGGGHWWLTGFKMGSVNPNEMVMSLK
                     IRMRSRKMANAFENGLQTAGFYRGDATSNYDSYYRNDTEFYILWYHTGNRNY"
     gene            2033..3277
                     /locus_tag="ACZPE2_01750"
     CDS             2033..3277
                     /locus_tag="ACZPE2_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR1318603.1"
                     /translation="MSEIKDIGLYESGERKIQWVKANMPLLRALEEEFSADKPFKGLK
                     VALSVHLEAKTAYLCRVLAAGGANMYITGSNPLSTQDDVAAALVKGGLNVFAKHGASA
                     EEYHAHIEKVLECGPNVIIDDGGDLVNTIHNEYPDLTSGVIGGCEETTTGIIRLKAME
                     NDGKLRFPMVAVNNAKCKYLFDNRYGTGQSVWNGINRTTNLIVAGKTAVVAGYGWCGK
                     GVAMRAKALGAKVIVTEIDKIKAMEAVMDGFSVMPMVEAAKYGDFFITVTGCDNVITE
                     KAFLNMKDGAIVCNAGHFNVEINIDDLERLATEKELKRDNIMGYKMPTGAWVNLLAEG
                     RLVNLAAGDGHPAEIMDMSFALQALSARYAVENRDKLKDGVNVVPEEIDCRVAEMKLA
                     SWGITIDALTPEQEKYINSWNV"
BASE COUNT          874 a          801 c          770 g          844 t
ORIGIN      
        1 ccttgcagac ctttttgatt tcgcgaattg tgtaattcgt catttccctg acttttgagg
       61 gataattcgg attgctttcg cttgctttca ttaaatcagc tccctaaagt tattcccaat
      121 tcacttaatt tacatttgtc tattatttta gcacactttc tcggataagt cgagaaaaaa
      181 ttttaatttg accgctcagg aatgatgttt ttgcaaaaaa cagcggacac cgttttccga
      241 aaagtccttt aaagcaatca tttaagctta tcgagcggca gctcgtatgc aggaataaat
      301 tcctccaaaa atatatgtgc ttccctacat tcgctctcgt cgagcgcttt tcttattgtc
      361 ctttcggcat cctcaaattc acagttgcct gccttgcgtt cttctatgca cttgatgtag
      421 gcactcagct tatccgccgc ctttatcagc cgccacagct ccgcgtcgcg ttcctccttg
      481 aaaaacagcg gtgaaaactc ctcgctcaaa tcgtccggca gcatattcaa aagcctttgg
      541 caggcctcct tttcgaccct accgaaaacg gagcgcatct ctctgctgta atattttacg
      601 ggagtgggca tatctccagt tatgatttcg ggggtgtcgt gataaagccc caaaagagcg
      661 gcgcgctcgg cattgtattc tttgccgagt cgcttatttc ctataacggc gagggcgtgc
      721 gctattgtgg caacctcgcc cgagtgctga ctcaaatttt catatgttgt gttgcgcata
      781 agtgcccagc ggtttatata tttgagcctt gaagtaaacg caaaaaactt gcctttcata
      841 aatatctcct ttaaagaaaa atcaggctac cccaaaactg agatagcctg tgttaagccg
      901 attatcaata attacgattg ccggtatgat accagagtat gtaaaactcc gtatcgttgc
      961 ggtaataaga atcgtaattc gaggtagcgt cgcccctata gaagcccgca gtctgaaggc
     1021 cgttctcaaa agcgttcgcc atttttcttg aacgcattct gattttaagg ctcataacca
     1081 tttcattggg atttacggag cccatcttaa agccggtgag ccaccaatgg ccgccgcccg
     1141 tccacgcatc gcgatagaaa aatctgttct tcacggcata tgtattagga gtcaaataca
     1201 aatcgtaata catattgagc aaatggtcgt tgtcggcaca attgtaaagc ttatcgttgg
     1261 tctcggggtc ctttgtgtaa acgccgacct caccgccgcc gaaggggccg tactgaccct
     1321 tccaaagctg aattctccaa tcaagaccgt catagtagaa atcaacggtc gttgtgaggt
     1381 atctcatatt accgaaaatt gctgcgtact tgtcgtactg actgataaag ttggcatctc
     1441 tctgccagga atccataagc gaataaaaaa tgccctgctc tgcatcgtaa ccgaagccca
     1501 tattgtttac aaaagcagcc aaagtctcgg gatcggcgtt cgcaagagca ccgagcaccg
     1561 cagcgcccaa agagcccata gaacttgcgg cgctgtttcc gcttgcagat gttgtgttgt
     1621 taaaatcagg cgttgtgacc gacggaacat tcagcgagga cgaagctttt gccgctgccg
     1681 cggttgccgc cgcggtggtt tcaccgccgg tggtctcttc gccggttgtg gcctcgccgt
     1741 tttcggctgt ggtcacgggg cccgctataa cctgattact tctcgttatt tcctcgtcgc
     1801 cgcccttttt tgacttgcat gacgaaaaaa cacaagctat cataataagc gcaataactg
     1861 cggatgttat ccttacagac atacttttca ttttatcacc atccgttaga ttataaatca
     1921 agagtattat tgcacacttg atttgtaaag tcaacagata aagcaatttt aatttgccta
     1981 tctctgtctt ttatagtata attgaacaat aaaattttag gtggtacaaa atatgagcga
     2041 aataaaagac atcgggcttt acgaatccgg tgaaagaaaa atacagtggg taaaggcgaa
     2101 tatgccgctt ttgcgcgcac tcgaggagga attttcggca gacaagccct ttaagggact
     2161 taaagttgcg ctgtcggttc accttgaagc caaaaccgca tatctctgcc gcgttctcgc
     2221 cgcaggcggc gcaaatatgt atattacggg cagtaatccg ctctcaactc aggacgacgt
     2281 tgccgcggca ctcgtaaagg gcggactcaa tgtatttgca aagcacggcg catcggcgga
     2341 agaataccac gcgcacatcg aaaaagtcct cgaatgcggc cccaacgtga taattgatga
     2401 cggcggcgat ctcgtaaaca ccattcacaa cgaataccct gacctcacaa gcggcgttat
     2461 cggcggctgt gaggagacaa ctacgggaat tatccgcctc aaagccatgg aaaacgacgg
     2521 taagcttcgt ttcccgatgg ttgccgtgaa caacgccaag tgcaaatatc tgtttgacaa
     2581 ccgatacggc acgggtcagt ccgtatggaa cggcataaac aggacaacaa atctgatagt
     2641 tgccggcaaa accgcggtag tggcaggcta cggctggtgc ggcaagggcg tcgcaatgcg
     2701 tgccaaagct ctcggcgcaa aggtgattgt cacggaaatt gacaaaatca aggcgatgga
     2761 ggcggttatg gacggcttct ccgtaatgcc tatggttgag gcggcgaagt acggcgattt
     2821 ctttataacc gtgaccggct gtgacaatgt catcaccgaa aaagcgttcc tcaatatgaa
     2881 ggacggtgca atagtttgta acgcaggtca cttcaacgtg gaaatcaaca ttgacgacct
     2941 cgagaggctc gcgacggaaa aggaattaaa gcgcgacaac ataatgggtt ataaaatgcc
     3001 gacgggcgca tgggtaaacc tcctcgccga gggcagactc gtaaatcttg ccgcaggcga
     3061 cggccatcct gccgaaatta tggatatgag ctttgccctg caggctctct ccgcgcgcta
     3121 cgccgttgaa aatcgcgaca agctgaaaga cggtgtaaac gttgttccgg aggaaataga
     3181 ctgccgcgtt gcggaaatga aactcgcgtc atggggtata acaattgatg cgctcacccc
     3241 ggagcaggag aaatacataa acagctggaa cgtataatat acaatatat
//
LOCUS       DBMDVO010000072         3284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13167_length_3284_cov_1.174050, whole genome shotgun sequence.
ACCESSION   DBMDVO010000072 DBMDVO010000000
VERSION     DBMDVO010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13167_length_3284_cov_1.174050"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="ACZPE2_01755"
     CDS             <1..1760
                     /locus_tag="ACZPE2_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318604.1"
                     /translation="DADVEAIPSGSSFALGNHNVTVYGAYKKKHKTLDMSAPLGTLYY
                     YNGTNIKKAVDSEFKANRDFSKYPAEAWKTYWTALTAAAKLVYGPFKKANLGNYSDDN
                     LTAAITALETAVKALDTASTTPSSGSATVTPAPIEAALKAAGDINYQNFDLYAYWAYE
                     KAMKAGNAIIDAYKGPVAPEKYIDGSSLSEAEITAIANKATATYKRAIVASMKAPSIE
                     AQNAYMDAVKAYKAPSYSELEVRNSAKNIAWYASFLKSAAVKTEKQFLDKEIKAAKAQ
                     DYKEADYTAGSYARYTKALAAAEALNANAKALQSEVFDAKYELEIAQRALMPKSASAL
                     EAGAYTELEAVIAQAKSIFTDNSAYTFDASKADGLSETEAYAKLVSVLGYEYTDEKGN
                     TANLYSGSAENYAANDRFYSNVVAAQIDAVVTNLKNAMAPFVCKYVAVPTTAGSNEGV
                     SVTENTSLITGVTPGSLATADDVLARVTAKDSSATTLKVAANAAGLYGTGATATLSLK
                     SSGAPVAIYTVVIYGDVNGDGVVDGFDASSMDLAINNKAALTGAYKTAGGLATGKVDL
                     ANYGLVVDAAYGGTAIAQK"
     gene            2048..3154
                     /locus_tag="ACZPE2_01760"
     CDS             2048..3154
                     /locus_tag="ACZPE2_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR1318605.1"
                     /translation="MTKAKKAISLIVSVVMLLGVLAVGVFANDNTGVAENGTIALSFD
                     KASYSAGDTVRVTVKLTTDYYAATVSVPVQYDSSAVKFVSGTTASTLFGAGDATKTVV
                     NDAAAGGKTYVYVGVLPQAAGGAVAQKANGVVLSTLTFTASKAISDTAAAFGVLNDQK
                     SMTNLGGKLYVGSYKTSDVTSTVYTTGQKLTFPVMKAAAAADPELQLSETGKANGVII
                     DRVSTCTDGNGFVYGVATDNGDSIADALQTPAGSIQVTENDQGQMSTGAKIELLNDSG
                     EVVETYYFIFLGDVNGDGFIDATDVTLAEGIASYSDFDFSADDGSVTGMAADINGDGF
                     IDATDVTYLEGVASYSDFPTQAETAAAVAAAKGI"
BASE COUNT          873 a          778 c          771 g          862 t
ORIGIN      
        1 cggatgcaga tgtagaggct attccttcag gttcttcatt tgctctcggt aatcacaatg
       61 taacagttta cggtgcatac aagaaaaagc ataagacact tgatatgtct gctcctctcg
      121 gcacactcta ttattacaac ggcaccaata ttaagaaggc cgttgacagc gagttcaagg
      181 ctaaccgtga tttttctaag tatccggcag aggcttggaa aacttactgg acagctctta
      241 ccgctgccgc taagcttgtt tacggcccgt tcaagaaggc taacctcggc aattacagtg
      301 acgacaatct cactgcagcc ataaccgctc ttgaaaccgc cgttaaggct cttgatacag
      361 caagcacaac accttcaagc ggttcagcaa ccgttacacc ggctcccatt gaggctgctc
      421 ttaaggctgc cggcgacatc aactatcaga acttcgacct ctatgcttat tgggcttacg
      481 aaaaggcaat gaaagccggc aacgctatca ttgacgctta caagggcccc gtagctcccg
      541 aaaagtacat tgacggcagc tcactttcag aggctgaaat tactgctatt gccaacaagg
      601 caaccgctac atataagaga gctatcgtag cttccatgaa ggctccttca atcgaagctc
      661 agaacgctta tatggatgcc gtaaaggctt acaaggctcc cagttattcg gagcttgaag
      721 tccgtaacag cgctaagaac attgcttggt atgcaagctt cctgaagagc gctgcggtta
      781 aaaccgaaaa gcagttcctt gataaggaaa tcaaagccgc taaggctcag gactacaaag
      841 aggctgatta cacagcaggc tcttatgcaa gatataccaa ggctcttgca gctgccgagg
      901 cattaaatgc caacgctaag gctcttcagt ccgaggtatt cgatgctaag tatgagcttg
      961 aaatcgctca gcgcgctctt atgccgaaat cggcttctgc tctcgaggca ggtgcttaca
     1021 ccgagcttga ggcagttata gctcaggcta agtcaatatt cactgacaac agtgcatata
     1081 catttgacgc ttctaaggct gacggccttt cagagaccga agcttatgct aagcttgttt
     1141 ccgtactcgg ctatgagtac accgatgaga agggcaacac agcaaacctt tacagcggct
     1201 ccgctgaaaa ctatgctgca aatgaccgct tctattcaaa cgttgtagca gctcagattg
     1261 acgctgttgt cacaaacctc aagaatgcta tggctccgtt cgtatgcaag tacgtagcag
     1321 ttcccaccac agcgggttca aatgagggcg tttccgtaac agagaacact tctctcatca
     1381 ccggcgtaac acccggctcg cttgctactg ccgacgacgt actcgcaaga gtaacggcta
     1441 aggattcttc ggctacaact cttaaagttg ccgctaacgc tgctggcctt tatggtacag
     1501 gcgctaccgc aacactcagc ctcaagtcga gcggtgctcc ggttgctatc tacacagtcg
     1561 ttatctacgg cgatgtaaac ggcgacggtg ttgttgacgg attcgatgct tcctccatgg
     1621 acctcgcaat caacaacaaa gctgctctca caggcgctta taagactgca ggcggccttg
     1681 ctaccggtaa ggttgacctc gctaactacg gccttgtagt tgacgctgct tacggcggaa
     1741 ctgcaatcgc tcagaagtaa tttcctttaa ttacttaagt taaaacccgg tgggggagtt
     1801 ttctccccca caagggcgta tgtaatggga tattcttaaa aagattagag catatacttt
     1861 aaaagtgcgg cggaatagtt ttgtgaaaaa acggtacaaa gcttgtaaag ctcgcgagga
     1921 aataacttta tttgtgatag cgtaagggat attttctttc ggtgtgccat ttttattcac
     1981 aaattattga cattgtgctc tcaaaagtgt atatttaata ttgattaaga atagaaggag
     2041 gcaaaaaatg accaaggcaa aaaaggctat tagccttatt gtatctgtag ttatgctcct
     2101 tggcgtgctt gcagtcggcg ttttcgcaaa tgataatacc ggtgtcgcag aaaacggcac
     2161 tatcgcttta tcgtttgaca aggcgtcata tagcgcaggc gatacagtca gggttaccgt
     2221 aaaattaacc acagattatt atgctgcaac ggtttctgtt cctgttcagt atgacagcag
     2281 tgctgttaag tttgtaagcg gcacaaccgc ttcaacgctg ttcggtgcag gcgatgcaac
     2341 aaagacggtc gtaaatgatg ctgctgcggg cggcaagact tatgtctatg tcggcgtatt
     2401 accgcaggct gccggcggag ctgttgctca gaaggcaaac ggcgtcgtac tttcgaccct
     2461 cacctttacc gcttctaagg ccatttccga tactgcggcc gctttcggtg tgcttaacga
     2521 tcagaaatca atgaccaatc tcggcggtaa actctatgta ggttcctaca agacctctga
     2581 tgtaacctca acggtttata ccacaggtca gaaacttact ttcccggtaa tgaaggctgc
     2641 tgctgcagct gatccggaac ttcagctctc tgaaacagga aaagctaacg gtgttataat
     2701 cgacagagtt tcaacttgca ccgatggcaa cggtttcgtt tacggtgttg caacagataa
     2761 cggtgattcc attgctgatg ctttacagac ccccgcaggt tcaattcagg taactgaaaa
     2821 cgaccagggt caaatgagca caggcgcaaa aatcgagctt cttaacgata gcggcgaagt
     2881 tgttgaaact tactatttca tcttcctcgg tgatgttaac ggcgacggat ttatagatgc
     2941 taccgacgtt acacttgctg aaggtattgc tagttattcc gatttcgact tttcagctga
     3001 tgatggctcg gtaaccggta tggctgctga catcaacggt gacggcttta tagatgctac
     3061 tgatgtaact tatttagagg gcgttgcgag ttactccgat ttcccgactc aggcagagac
     3121 agctgctgca gttgctgctg caaagggaat ataagtttat aattcatttt ataggaggta
     3181 aatcagatgc gcagaaaagt gctttctgtt attatggctg ttgtcatgat gatttcctgc
     3241 tttgcgctta tggcgagtgc ttacacttat agcccctcgt taac
//
LOCUS       DBMDVO010000073         3274 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13228_length_3274_cov_1.209581, whole genome shotgun sequence.
ACCESSION   DBMDVO010000073 DBMDVO010000000
VERSION     DBMDVO010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13228_length_3274_cov_1.209581"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="ACZPE2_01765"
     CDS             complement(<1..219)
                     /locus_tag="ACZPE2_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1318606.1"
                     /translation="MGNFDIRVKRFIDVFINAGAYNKVLLGLKNTIIIAVLGLVIGIL
                     IGTLIATIRVLPKYRRLPRILNGFCSFYV"
     gene            complement(301..1194)
                     /locus_tag="ACZPE2_01770"
     CDS             complement(301..1194)
                     /locus_tag="ACZPE2_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1318607.1"
                     /translation="MKKALKVISLMLVAVLMVMSFAACGSKDDTATGSDTSKEAKTPA
                     VKVIDVNLTDEEYAFGVDKNQPELLEKVNSFIAEIKANGTFDAICNKYFAGGTPDVIK
                     SAALDSTKDQLVVATNAAFEPFEYTNGDGYSGIDMEIAKALADYLGKELVIQNMDFDS
                     VCLSVGQHKCDVAMAGLTVKAEREEYVTFSTPYYKASQRLVVKGSDTKFDGCTDVASV
                     EAILNTLDSSVKIGVQNGTTGQLYCEGDAEMEFPGLKAVCVGYKSGSLAVQDMINGNI
                     NYVIIDAAPAACIAQSINEVN"
     gene            complement(1317..2813)
                     /locus_tag="ACZPE2_01775"
     CDS             complement(1317..2813)
                     /locus_tag="ACZPE2_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318608.1"
                     /translation="MVTQNSGSSVDTESVKTKRRGLFSKEADEGYVPNRELFAYSAAL
                     AGQNLTYQFVTQWLFYFCTDVLKIGARKVGFFTGFSRIWDALNDPIVGAVIDRRRCKN
                     GKKLHPYLGKLPIFIGILTALMFVDFGVGETAAMVIILCVYIGWDFTYSFQDVALWGM
                     MSLISPHPHERARVSQWLNIGVGAAWGAISLIPMIMGAREKIGISEKLLFLIFGIVFG
                     LGGELMSILAYKTKERVEFVAPPKQSLLKDIALLRYNKILILLLLAQILNFFNGAIAW
                     IYFFKYCVSVNIGGKVINGETVQFYYGILVSAFSAVSMFFAVKLADKLGGMKNMVVLA
                     QVMNAAFRVVAFFIGFDTLPKMIAVIAIISISSIPTNMVGIAQRALLCDSIDYTEWKT
                     GKRTEGISNSMQNLTNKFVDALKLIFSGLVLSGLGYDANLGLEGQTQAFYKAQWPLFM
                     LLPALGSVLYLIPFLCIRYSKAQRAQVEKELAERHAESLANDETESVK"
     gene            complement(2875..>3274)
                     /locus_tag="ACZPE2_01780"
     CDS             complement(2875..>3274)
                     /locus_tag="ACZPE2_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318609.1"
                     /translation="KLPCGLNAMENAGVRANSEEGEVVSTIAIATSSIVTTVIIALGV
                     LVFSPLLPYITAEDSPLTPAFNQVVPALFGALGISYFRKHWKISIIPLAVIVLVLLIN
                     GSVGSGVLIPIGVVVALLSTHLLYKKGWVK"
BASE COUNT          933 a          776 c          685 g          880 t
ORIGIN      
        1 tacatagaag ctgcaaaagc cgtttaaaat acgaggcagt cttctgtatt tcggcaaaac
       61 tctgattgtg gctataagag tacctataag tataccgata acaagaccca atacagctat
      121 tataatagta tttttaaggc ctaacagaac cttgttatag gcacctgcgt ttataaatac
      181 atcaataaag cgcttgaccc taatatcaaa attacccatg tatcaaaatc cttactttaa
      241 tataaacaat gccccacttt tattaggtga ggcattgaag tgttaagtct taaccgcagt
      301 tcagtttact tcgttgatgg actgagcaat gcaagccgcg ggagcagcat caattataac
      361 ataattgata ttgccgttga tcatatcctg aacggcaaga gaaccgctct tgtagcctac
      421 acaaacagct ttaagtccgg ggaactccat ctccgcgtcg ccctcgcagt aaagctgacc
      481 tgtggtgccg ttttgaacgc ctatttttac actgctgtca agagtgttga ggatagcctc
      541 aacagaagcg acgtcggtgc aaccgtcaaa ctttgtatcg gagcccttaa ctacaagtct
      601 ctgagaagcc ttgtaataag gagtagagaa agtgacatac tcttctctct cggcctttac
      661 ggtaagacct gccattgcga catcgcactt gtgctgacct accgagaggc aaaccgagtc
      721 aaagtccata ttctggataa cgagctcttt gccgagataa tccgcaagag cctttgctat
      781 ttccatatcg ataccgctgt aaccgtcgcc gtttgtatac tcaaacggtt caaaagccgc
      841 gttggtggca actacaagct gatccttggt agaatcaaga gcagctgact tgattacatc
      901 gggagtgccg ccggcgaaat atttattgca tattgcgtcg aaagttccgt tagctttgat
      961 ttcggcaata aagctgttga ccttttcgag aagctcgggc tgattcttgt caacgccgaa
     1021 cgcatattct tcatccgtaa ggttaacgtc gattacctta actgccggtg tttttgcttc
     1081 cttggacgta tccgagccgg ttgccgtgtc atccttgctg ccgcaggctg caaacgacat
     1141 aaccattaaa acggctacga gcataagcga aattactttt aatgcttttt tcattacgat
     1201 tcctccgtaa taatttaatg gatatataat atcactatct gtcaaattat gcaagagtaa
     1261 tgtataatta ttcgattttg cacgaattat atacgtaaaa caccgtatta ttatgtttat
     1321 ttgacggatt cggtttcatc atttgcaagt gactccgcgt gtctttccgc aagttccttt
     1381 tcaacctgcg cacgctgagc ctttgaataa cgtatgcata aaaacggaat gagatagagc
     1441 acagaaccaa gtgccggaag gagcataaag agcggccact gagccttata aaaagcctgc
     1501 gtctggccct caaggcccaa attcgcgtcg tagccgagcc cggaaagtac aagtccgctg
     1561 aatatcagct ttaaggcgtc tacgaatttg ttcgtcaaat tctgcatgga atttgatata
     1621 ccctcggttc gcttgccggt tttccattcg gtatagtcta tggaatcgca aagaagcgca
     1681 cgctgagcta taccgaccat attcgtcggt atggacgaga tggatattat ggcgattacc
     1741 gctatcatct tggggagcgt gtcaaagcct ataaagaaag ccacgactct gaaagcggca
     1801 ttcataacct gcgccagtac gaccatattt ttcataccgc cgagcttatc tgcaagcttt
     1861 acggcaaaga acatacttac ggcggaaaac gcactgacaa gtatgccgta atagaattgc
     1921 accgtttcac cgtttataac cttgccgccg atatttacgc tgacgcagta tttaaagaaa
     1981 tatatccacg ctatggctcc gttgaagaag ttgagaatct gcgcaagcaa gagtaaaatg
     2041 agtattttgt tgtaccttag caacgcaatg tcttttagaa gcgactgctt aggcggtgca
     2101 acaaattcaa ctcgctcctt agtcttgtat gcaagtattg acatcagctc gccgcctaaa
     2161 ccgaatacaa taccgaaaat aaggaacaac agcttttcac ttattcctat tttttctctt
     2221 gcgcccatta tcatcggaat aaggcttatt gcgccccacg ccgctccgac gcctatgtta
     2281 agccactgag aaaccctagc tcgctcgtga gggtgcggcg aaatcaacga catcataccc
     2341 cacagggcaa catcctgaaa tgaatatgta aaatcccagc cgatataaac gcagaggatt
     2401 attaccatgg ccgcggtttc tcccacgccg aaatccacaa acataagagc agtcaatatg
     2461 ccgataaaaa tcggcagctt accgagatac ggatgcagct tcttaccgtt tttacatctt
     2521 ctgcggtcaa ttaccgcacc tactatcggg tcgttcagag cgtcccaaat tcgtgaaaat
     2581 ccggtgaaaa atccgacttt tctggcacct attttaagta cgtcggtaca gaaataaaac
     2641 agccattgtg ttacaaactg ataagtgaga ttttgcccgg cgagagccgc agaataagca
     2701 aaaagctctc tgttcggtac atatccttca tcggcttctt tgctgaaaag gccgcgtctt
     2761 ttggtcttta cgctttcggt atcaaccgac gagccgctgt tttgtgtcac cataagataa
     2821 ttcctcccaa aacaatctta atacactttt tctgtttaat taaatcgcct gcaattattt
     2881 tacccagccc tttttataaa gaagatgagt gctgagcagg gcaacaacaa cacctatggg
     2941 tataagcact ccggaaccta cggagccatt gataagcaaa actaagacta ttacggcaag
     3001 cggtattata gatatcttcc aatgcttgcg gaaatagctt attccgagtg cgccgaacag
     3061 cgccggaaca acctgattaa atgccggggt aagcggcgag tcctctgctg tgatatacgg
     3121 aagtaaggga gaaaacacaa gcactccgag agctattatt accgtagtaa cgatagatga
     3181 agtggctatt gcgattgttg atacaacctc gccttcctcc gaatttgcgc gaacgcccgc
     3241 attctccatg gcattgagac cgcacggaag ctta
//
LOCUS       DBMDVO010000074         3273 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13235_length_3273_cov_1.139029, whole genome shotgun sequence.
ACCESSION   DBMDVO010000074 DBMDVO010000000
VERSION     DBMDVO010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_3273_cov_1.139029"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="ACZPE2_01785"
     CDS             <1..502
                     /locus_tag="ACZPE2_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024122.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIR1318610.1"
                     /translation="PWILVLAPFQIIMALLLVTKIISFTTFKKYFFCFVMLVPLNKAV
                     NKFWDKHEKEVFPWFKERKRYAVVISASPDFLLCEIQKRLKFETLICTKHNPKTGAII
                     GLNCRDEEKVRRFRELFPDAEVTDVYSDSLRHDRPIFSLGKHCFHIVRGERREFNYES
                     VYGSKK"
     gene            complement(577..1086)
                     /locus_tag="ACZPE2_01790"
     CDS             complement(577..1086)
                     /locus_tag="ACZPE2_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1318611.1"
                     /translation="MVQLYYGFGKGKTSAAAGAAVRAAGNGMRVIFAQFFKDGKSGEI
                     KVLRDIPGITVLLPKSDYKLFENMTDMRKKEIFEDYTKLLRSVFERENDCDMIILDEA
                     LDAYTGGFVDRELFLSCIKSCRDKLEIIVTGHAKSDDIADAADYVTEFTAVRHPYEKG
                     VKARRGIEY"
     gene            complement(1156..2982)
                     /gene="glmS"
                     /locus_tag="ACZPE2_01795"
     CDS             complement(1156..2982)
                     /gene="glmS"
                     /locus_tag="ACZPE2_01795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1318612.1"
                     /translation="MCGIVGYVGENSASPILIDGLKKLEYRGYDSAGIAVSENGKTDV
                     VKTKGRIKNLEEKLKEVGGLSGTVGIGHTRWATHGAPTDVNAHPHKGGNGRITLVHNG
                     IIENYIELKTMLQKGGYVFVSETDTEVLAHLFDYYYKGDLLDAMVNVMKTVRGSYAVA
                     ILANDKPGTIIAARKDSPLIVGLGDGENFLASDIPAVLKYTRDCYLLEDNQIAVLTKD
                     NVTLYDTNKNVVKKDVYHVTWDVEAAEKCGYEHFMQKEIAEQPKAIHDTLSPRIKDGR
                     VSIPELTLTDEEIKNIGKIHIVACGSAWHVGMTAKYIFEDMARIPCETDLASEFRYRD
                     PIIKKGDICIVISQSGETADTLAALREAKSKGAKVISIVNVVASTIARESDDVIYTMA
                     GPEIAVATTKAFSAQLAVVYLLALRFSKAVGLLPEEREKELTKELMCLPSQIEDLLNL
                     TDELKTLAHKYVGSDDVFFIGRGLDYAISLEGSLKLKEISYIHSEAYAAGELKHGTIS
                     LIEEGTPVFAVATQDKLFEKTLGNIKEVKARGASVIALVTDKHASVSEFADEVIRVPS
                     TYGNFLPSLNVIPLQIFAYYMAVLRGCDVDKPRNLAKSVTVE"
BASE COUNT          840 a          825 c          685 g          923 t
ORIGIN      
        1 cccgtggata ctcgttcttg caccattcca aataattatg gctcttttgc tggtcaccaa
       61 aattatcagc tttaccacat tcaaaaaata ttttttctgc tttgtgatgc tcgttccgct
      121 aaacaaagcc gttaataagt tttgggataa acacgaaaaa gaggtatttc cctggtttaa
      181 ggagagaaag cgatacgccg ttgtaataag cgcaagcccc gattttttac tgtgcgaaat
      241 ccaaaagcgg ctcaaatttg aaacgctgat atgcacaaag cacaacccca aaaccggtgc
      301 tattataggg cttaactgcc gcgacgagga aaaggtaaga cgcttcaggg agctttttcc
      361 cgatgccgag gtaacagacg tatattccga cagcctgcgt catgaccgtc ccatattttc
      421 gctcggaaag cactgtttcc atatagttag gggtgagcgc cgcgagttta actacgagag
      481 cgtttacggc agcaaaaaat aaataaagtg ttttataaaa accaaggagc tgtaaaaaac
      541 ggaagttttt tacggctcct ttttgcagta tattgttcag tattctatgc ctcggcgagc
      601 ttttacgccc ttttcgtaag ggtgccgaac ggcggtaaac tccgtaacgt aatcggcagc
      661 gtccgctata tcgtcgcttt ttgcgtgacc cgtcactata atttcaagct tgtcgcgaca
      721 gcttttgata cagctcaaaa aaagctcacg gtcaacaaat ccgcccgtgt atgcgtcgag
      781 agcttcgtcg aggattatca tatcacaatc attttcgcgc tcaaaaacag agcgaagcag
      841 cttcgtgtaa tcctcaaaaa tctctttttt tcgcatatcg gtcatattct caaacagctt
      901 gtagtcgctt ttcggcaaga gcaccgtaat accgggaatg tctcgcagca cttttatttc
      961 gcccgattta ccgtccttaa aaaactgtgc gaatataacc ctcattccgt tgcccgcggc
     1021 tcttacggcc gcgcctgccg cagcggaggt ttttcctttg ccgaagccgt aatacagctg
     1081 taccatttat ctacctgcct ttatgcttta ggcttttatt atacagaaaa tccataagaa
     1141 aagcaatatt cacggttact ccacggtgac ggattttgcg agatttcgcg gcttatcaac
     1201 atcgcagccg cgaagcacag ccatataata agcgaatatc tgaagaggta ttacattgag
     1261 cgacggtaag aaattaccgt atgttgacgg tacgcgtata acctcgtcgg caaattccga
     1321 aacagaggca tgcttatctg tgacaagtgc gattaccgac gcgccgcgcg ccttaacctc
     1381 tttaatatta ccgagagttt tttcaaaaag tttatcctgc gtcgcaacgg caaacacggg
     1441 agtcccctct tcgataagag atatggtgcc gtgcttgagc tcaccggcgg cataggcctc
     1501 cgaatgaata tacgatattt ccttgagctt taacgagcct tcaagagaaa tggcgtagtc
     1561 aagccccctg ccgataaaaa acacatcatc ggagccgacg tatttatggg cgagcgtttt
     1621 aagctcgtcg gtaagattta aaaggtcttc aatctgcgac ggaagacaca taagctcttt
     1681 tgtgagctct ttttctcttt cctcgggaag aagccccaca gcctttgaaa aacgaagtgc
     1741 aagcagatat actacggcaa gctgtgccga aaaagccttt gttgttgcaa cggcgatttc
     1801 gggtcccgcc atggtatata taacgtcgtc gctctctctc gctattgtgc tcgcgaccac
     1861 atttactatc gaaatcacct ttgcaccctt gcttttagcc tcacgcagag ccgctaaggt
     1921 atcggcagtt tcaccggatt ggcttataac tatgcagata tcgccctttt tgattatcgg
     1981 gtcgcgatag cgaaattcgc ttgcaaggtc tgtttcacat ggaatacgcg ccatatcctc
     2041 gaatatatat tttgccgtca tacctacatg ccatgcgctg ccgcacgcca ctatatgaat
     2101 cttgccgata ttcttgattt cttcatcggt aagggtgagc tcgggaattg acaccctacc
     2161 gtccttaatt ctcggcgaaa gcgtatcgtg tatagccttg ggctgctcgg caatttcctt
     2221 ttgcataaag tgctcgtagc cgcacttttc cgcagcctca acgtcccatg ttacgtggta
     2281 aacgtccttt ttgacaacat ttttgtttgt atcgtaaagc gtcacattgt cttttgtgag
     2341 aaccgcaatc tgattgtctt caagcaaata gcagtctctt gtatatttta aaaccgcggg
     2401 gatatccgac gcaaggaaat tctcaccgtc gccgagcccc actataagcg gactgtcttt
     2461 tcgtgccgct ataattgtac cgggcttatc gttcgccaaa atcgcaacgg cataagagcc
     2521 gcgcacggtc ttcataacat ttaccatggc gtcaagcaaa tcgcccttgt agtaataatc
     2581 aaacaaatgc gcgagcacct cggtatccgt ttcggaaacg aatacatagc cgcccttttg
     2641 gagcatagtt ttaagctcaa tataattctc gattataccg ttgtgcacaa gcgttattct
     2701 gccgtttccg cccttatgcg gatgtgcatt gacatctgtc ggcgcaccgt gggtggccca
     2761 tcttgtatga cctattccga ccgtgccgga aagaccgccg acctctttga gcttttcctc
     2821 aagatttttt atacggcctt ttgtcttaac aacgtctgtt ttgccgtttt ctgacacagc
     2881 aatgcctgcc gagtcataac ctctgtactc aagctttttt aaaccgtcaa tcaatatcgg
     2941 agaggcacta ttctccccta cataaccaac aattccacac atattaaatt tttccttttc
     3001 aaaaaataaa atttgtgcct tgctttccct ttgtacacgg tgcgttgccg ctccgccttt
     3061 gtgaaaagcc tttgatcccc gcacttatgg gacaagaagc atccgccgaa aacttcgata
     3121 acttcttcct cgtcacctgt aaattcggca aagccgatag tgcgacatac aggtccggcg
     3181 ctaaaactct cttgcggaaa gtcctttaaa aaacaaaaat ctcctttcgc ttttttgagg
     3241 gcggctcgca cagccgacgg ctcatttgtt aaa
//
LOCUS       DBMDVO010000075         3214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_13595_length_3214_cov_0.999036, whole genome shotgun sequence.
ACCESSION   DBMDVO010000075 DBMDVO010000000
VERSION     DBMDVO010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13595_length_3214_cov_0.999036"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="ACZPE2_01800"
     CDS             complement(<1..1420)
                     /locus_tag="ACZPE2_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318613.1"
                     /translation="MKEKKSIKKLIAFFGTVLAIMALMCTAAFAEISETDKAAIGGTG
                     YATFDAAKNAAKDGDTITLLADAEVDRSNFDTYQIWYRITVDGQNKYGLKINGTGSVE
                     IQVEVHFKNMVINDTATHYHGIFKITGSGHLVLENSTVTGAVGGVAGPIVSESKDARV
                     TLIGSKIDSSKSSWNNNKGTAVSMSKGGKLAIDANSEITGCQNGSAAVAVYGGAGSIE
                     VNGKITGNTSVTDGAVYAGSGVTVTIGENAEISGNGSNLIAVKADDENAITDNSGKYG
                     KYGNNYYEESAVVISDSGTAYSDIYNSLKNAGTMYLLKDGVFTKKNGNTDKGNLDTGI
                     SVTLDGQNNTVTFETGTAVAQDITFKNVTLDISNLSGDAFSIWGGSVTLESGAVVTGN
                     GQAAAGERRFVTANANTAFNMNKGSSVTGVINSTALGGAVQLDGGVFNMTGGTISGCK
                     ADAGGAVCIKGGKMVMSGGTISN"
     gene            complement(2561..>3214)
                     /locus_tag="ACZPE2_01805"
     CDS             complement(2561..>3214)
                     /locus_tag="ACZPE2_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR1318614.1"
                     /translation="APAGYTFKGYYLDEALTVPYANDGVKANITLYPAFEKNRARTDL
                     TDIAGHWAEEYIASLYEKSIVSGTGDGKFNPDSNITRAEFIQILYNMSNMTSDGSQSF
                     KDVKAGDWFSQAVAWAVNFGITSGTSEDTFSPYEKITREQMAAMIYRYATLMGADWQT
                     SETGEFADEGEIADYAKYQVRWAKGAGIISGRPDGSFGPKDNATRAESAAMLSRLVK"
BASE COUNT          706 a          871 c          755 g          882 t
ORIGIN      
        1 tattactgat tgttccgccg gacataacca tctttccgcc ttttatgcat accgcaccgc
       61 cggcgtctgc cttacatccg ctgatcgttc cgccggtcat gttaaagact ccgccatcaa
      121 gttgaaccgc accgccgaga gccgtgctgt tgatgactcc ggtaaccgac gaacccttgt
      181 tcatgttgaa ggccgtgttt gcattcgcgg taacaaaccg tctttcaccc gctgctgcct
      241 gtccgttgcc ggtaacaaca gcgccgctct caagcgtcac gcttccgccc cagatggaga
      301 atgcgtcgcc ggagaggttc gaaatgtcaa gtgttacatt tttgaacgtg atgtcctgag
      361 ccactgccgt gcccgtctcg aatgtcaccg tgttgttctg cccgtctaat gtcacgctga
      421 tgcctgtgtc aaggttgcct ttgtccgtat tgccgttctt ctttgtgaag acaccgtcct
      481 tcaaaaggta cattgtgccg gcatttttaa gcgaattgta aatatcggaa tatgctgtgc
      541 cgctgtccga gataaccacc gcagactctt cataatagtt gttgccgtat tttccgtact
      601 ttccggaatt gtcggtaatt gcattttcgt cgtccgcctt aaccgcgata aggtttgaac
      661 cgttgcccga aatttcagca ttttcgccga tcgtaacggt cacgccgctt ccggcataaa
      721 ccgcgccgtc ggtaaccgag gtgttgcccg tgatcttacc gtttacctcg atcgaacctg
      781 cgccgccgta aaccgcgacc gccgcacttc cgttttggca gccggtaatt tcggaattag
      841 cgtcgatagc aagttttccg cccttgctca tcgaaaccgc tgtaccctta ttgttgttcc
      901 atgagctctt cgaggaatcg attttgctgc cgataagcgt gacccttgcg tccttactct
      961 ccgacacgat cggacccgca acaccgccga ccgcaccggt aacagtgctg ttttcaagca
     1021 cgagatgacc cgagcctgtg atcttgaaaa tgccgtggta gtgagtcgcc gtgtcgttga
     1081 tcaccatgtt cttaaagtga acctcgacct gaatctcaac cgaacccgtg ccgttgatct
     1141 tgagcccgta cttgttctgc ccgtcaaccg tgatccgata ccagatctga taggtgtcaa
     1201 aattcgatct gtcgacctct gcgtccgcca agagggtgat cgtgtcgccg tctttcgccg
     1261 catttttcgc cgcgtcaaac gttgcatatc ccgtgccgcc gatagccgcc ttatcggtct
     1321 cgctgatctc ggcaaacgcg gcggtgcaca taagcgccat tattgccaga accgtgccga
     1381 agaaagcgat cagtttttta atgcttttct tttccttcac ttttgttccc ccgtcccttt
     1441 cataaattta aagtaattag ggtttgtttg gtttatgtgt aacataaacc aaagggtata
     1501 ccctttgact ctataataca attaaatttc agttttgtca ataggttttt tagatttctt
     1561 gtctatatta aaaatcttgt tcatatatat tgcttacaat ttaagatata ttgctcgttt
     1621 gtcttcattt tcattgaatt tgcctttttc ggtcagtttt tcaacgaatt caaagaggct
     1681 ataaaagggt aaaaatcccc tcgcaatgtg tgtgcgcgaa ccaaaaatca ggaggatgtt
     1741 catcatcctc ccctacggcg ccgaaggcaa tgaatgacag cacggtggac tcatcaagaa
     1801 ccgaggcgtg aggagagccg cagtgagacg gaggagccga aggcggaagg aggatattac
     1861 cacaggcaaa cttgcggtcg attcgtgaat cgaccctacg ggtgctctaa ctgaacggaa
     1921 aacataaaca ccggcgcgtc ggaaaccgcc gcgccctacg ggtgttctat ttttttaaac
     1981 gatgcaccga atatacggcc gcaaattcgt agagttctca cgtcctcgac gaaccgaggg
     2041 ggtgcgtaaa accatcggga tgtcggggac ttttatcccc tacgggtgct ctaaccgagt
     2101 ggagtgcacc catgccttcc tctgacgagg gaggtggttt ttatgaaata aaaaccggag
     2161 ggagagaagc caatggtgcg caataaaaaa gagccctgtg tgatttacag tctctccctc
     2221 ggtcattttc tttgaaaatg actgagggag cctttaaaat aatgcaccga tcgaagtgag
     2281 tgcgcaaaaa aatccctcag tctcgggcaa gccgagacag cataaaaccg caaaggcggt
     2341 tttatgagcg tgcgacaaaa ccttgaacgg tttttcgcac cggcccacaa aagactctgt
     2401 tgtctttttg tgggcatgcc cctcggggag cctttaaatg cgcactaacc gaggttacca
     2461 cacacaaaaa ggacggcgag tgactcgccg tccttttatt tactgtcctc ttacttgctg
     2521 tcctttactt tcacgggttt gcacaccgct aaagtacctc ttattttaca agtctcgaaa
     2581 gcattgccgc gctctctgct cttgttgcgt tgtccttcgg tccgaatgag ccgtcgggtc
     2641 tgccggagat gattcctgcg cccttcgccc atcttacctg atacttcgca taatctgcaa
     2701 tctcgccctc gtccgcgaac tcgccggtct ctgatgtctg ccaatctgct cccatcaagg
     2761 ttgcataacg gtagatcatt gccgccatct gctcacgtgt gatcttctca tacggcgaga
     2821 acgtatcctc gctcgtaccc gacgtaatgc cgaagtttac cgcccatgca accgcctggc
     2881 tgaaccagtc gcctgccttt acatccttga agctctgcga accgtcggat gtcatattgg
     2941 acatattgta aagtatctgt atgaactctg ctcttgtgat gttgctgtcc ggattgaact
     3001 ttccgtcgcc cgtgcccgat acgatcgact tctcataaag agacgcgatg tattcttctg
     3061 cccagtggcc ggcaatatcc gtaaggtctg ttcttgctct gttcttctcg aatgccgggt
     3121 aaagcgtgat gtttgccttt acgccgtcgt tcgcataggg aactgtgagc gcctcgtcaa
     3181 ggtagtagcc cttgaacgtg tagcctgccg gagc
//
LOCUS       DBMDVO010000076         3102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14347_length_3102_cov_0.886371, whole genome shotgun sequence.
ACCESSION   DBMDVO010000076 DBMDVO010000000
VERSION     DBMDVO010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14347_length_3102_cov_0.886371"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="ACZPE2_01810"
     CDS             <1..392
                     /locus_tag="ACZPE2_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR1318615.1"
                     /translation="GITYSPASSSQMYSMIVSLKDLPAGSLIVQSVQSDSSLASQDVK
                     QGDLITKVNGKDLDSADDLPDLIDKSAVGDTLTLTICRIDSNYNINEFEVKATLVEDK
                     GDSSSVKTESTTRSNSYNPFSAYGYGN"
     gene            complement(472..789)
                     /locus_tag="ACZPE2_01815"
     CDS             complement(472..789)
                     /locus_tag="ACZPE2_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318616.1"
                     /translation="MKKAVAAVLLAVLLVCAVFSSCGKKEDGVVTGNGNTTRSAAQNA
                     VTNAGDAVGDAVTGAGDVVGDVVTGAADIAGDVITGAGDIVNDAATGAGEAASKAVSD
                     AAR"
     gene            complement(859..1917)
                     /locus_tag="ACZPE2_01820"
     CDS             complement(859..1917)
                     /locus_tag="ACZPE2_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025971.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY1/2 domain-containing protein"
                     /protein_id="HIR1318617.1"
                     /translation="MLPLENTNLENTYKFLSQVGDFTATLNRKVASGESISDEERENL
                     RSLITFCDALSGEVSNIRADMFDGSFSFSQKSGELALTGEKTENLAGDMEDAQQSLSD
                     YPTLIYDGPFSDHIMSAQPKMTSGAKEISKENALDAAAAFLGCDKKEISFLSEESGNV
                     PAYCFSHNNKTVAVTKNGGYVIYMLDSSFAGEAKLKTADALKKASEFLASHGYADMKE
                     SYYSTSDGVCTVNYAYKKDGVIYYPDLIKVGVNLETGDIASFDAKGYIMNHTERNLSS
                     DILSQAEAQKSVSGLLTVLDSKRAVIPTKSKGEKDCWEFHCTDKDGNEVLVYIDTKTG
                     YEDDILLLLYSDGGILTK"
     gene            complement(1839..2201)
                     /locus_tag="ACZPE2_01825"
     CDS             complement(1839..2201)
                     /locus_tag="ACZPE2_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318618.1"
                     /translation="MEKKMKRRSYVRVVSFVAALAAVLVTASICGNVRASKYKRQITL
                     SQQRALTELDGRLREIGTSLKKGVYSSTPPMLSGMASELSRETLAAKARFPCFRLKIQ
                     ISKTHINFYRRSAISPRP"
     gene            2337..>2729
                     /locus_tag="ACZPE2_01830"
     CDS             2337..>2729
                     /locus_tag="ACZPE2_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1318619.1"
                     /translation="MECYLDNSATTKPCKAAVDAEISALTELYGNPSSLHQKGVYAYM
                     LLEKSRGTIAASLKASPDEIYFTPCGTVSNNTAIFGAVDAKKRLGKKIVTTALEHPSV
                     EKCMERLEESGFEVVRVQPQSDGNISLKD"
     assembly_gap    2730..2739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2740..>3102
                     /locus_tag="ACZPE2_01835"
     CDS             <2740..>3102
                     /locus_tag="ACZPE2_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1318620.1"
                     /translation="ENAIDENTILVSVMAVNNEVGSVMPTENLKKIIKSKNSPALLHV
                     DDVQGYMKIPLCPKSCGIDLMSVSAHKVHGPKGVGALYINKNVRINPYILGGGQENNM
                     CSGTQGMPAIAGFAAAVE"
BASE COUNT          817 a          787 c          688 g          800 t
ORIGIN      
        1 tcggaataac ctattcgccg gcgtcttcaa gtcagatgta cagtatgatt gtaagcctta
       61 aggatctccc ggcaggttcc ttgatagttc agagtgttca gtccgattca agccttgcgt
      121 ctcaggacgt aaagcagggc gacttaatta ccaaggtcaa cggcaaggac cttgacagcg
      181 cagacgatct tccggacctt atcgataaat ctgccgtggg cgatacgctt acgcttacga
      241 tttgcagaat agacagcaat tacaacatca atgaatttga agtaaaggca accctcgttg
      301 aggataaggg cgattcctct tccgtaaaga cggagtccac aacacgcagc aactcgtaca
      361 atccgttcag cgcatacggt tacggcaact gataagctgc cgcagcacaa gcgctccggt
      421 ttaaataaaa aagtgaagcc acggttagcg ctaaccgtgg cttcgtcaat tttatcttgc
      481 cgcgtccgac acagccttgc ttgcggcttc gcccgcacct gtcgccgcgt cgtttactat
      541 gtcgcccgca cccgtgataa cgtcgcctgc tatatcggcg gctccggtaa caacatcacc
      601 gacaacatct ccggcgccgg tgactgcgtc acctacggcg tcgcctgcgt ttgtaaccgc
      661 attttgcgcg gcggagcgag tagtatttcc gtttccggta acgactccgt cctccttttt
      721 accgcaggaa gagaacacgg cgcaaacaag aagcacggcg agcaaaacag ctgcaacagc
      781 ctttttcata tctatcacca caatctttct tttgtctcat ttcggacaaa attattattt
      841 ccgtattttt tctttttatt attttgtcag gattccgccg tcggaataca aaagcagaag
      901 tatatcgtcc tcatatcccg tttttgtatc aatatagacg agcacctcat taccgtcctt
      961 atcggtgcaa tgaaattccc agcaatcctt ttcacccttg ctctttgtcg gaattacggc
     1021 tctttttgaa tcgagcaccg ttaaaagtcc gctgacggat ttttgagcct ccgcctgtga
     1081 caaaatatca gagcttaaat tgcgctcggt atgattcata atatagccct ttgcgtcaaa
     1141 tgacgcaata tcacccgtct cgagatttac gcctaccttt atgagatcgg gataataaat
     1201 aacaccgtcc tttttgtagg cataatttac ggtgcagacc ccgtccgacg tgctgtaata
     1261 actctccttc atatcggcat agccgtgaga cgccaaaaat tcggaagcct ttttcagcgc
     1321 atcggctgtt ttcagcttcg cttcgcctgc aaaggacgag tcgagcatat atataacata
     1381 tccgccgttt ttggttacgg caaccgtctt attattatga gaaaaacaat acgcaggcac
     1441 atttcccgac tcttcgctta aaaacgatat ttcctttttg tcgcagccga gaaaggcagc
     1501 tgccgcatca agggcgtttt cctttgatat ttcttttgct ccgctcgtca ttttcggctg
     1561 agccgacata atatggtcgg aaaacgggcc gtcataaata agcgtgggat aatcgctcag
     1621 cgactgctgt gcgtcctcca tatcgcctgc aagattctcc gtcttttcgc ccgttaaggc
     1681 aagctcgccg cttttttgtg aaaagctgaa gcttccgtcg aacatatccg cgcgtatatt
     1741 tgaaacctct cccgacagcg catcgcaaaa cgtgataagg gagcggagat tttcacgttc
     1801 ctcgtcggag atgctttcgc ctgaggcaac cttgcggttt aaggtcgcgg tgaaatcgcc
     1861 gacctgcgat aaaaatttat atgtgttttc gagatttgta ttttcaagcg gaagcacgga
     1921 aagcgagctt ttgccgcaag agtctctctt gacagctccg acgccatacc gctcagcatc
     1981 ggcggagtgc ttgaatacac cccttttttg aggcttgtac ctatttcacg cagtcttccg
     2041 tcaagctctg taagtgctcg ctgctgcgac aaagtaatct gtcttttata ttttgacgcg
     2101 cgcacattgc cgcatatcga cgctgtgaca agcaccgccg caagtgctgc gacaaaggat
     2161 acaacgcgca cataggagcg tcttttcatc ttcttttcca taaatttcac ctctgcgata
     2221 tttttcgcaa aaaaaacata aatatacttg aaaaaagccg cttatttatt tgccaaaccg
     2281 caccttatag tgtataataa tatactgatt aagtttgaat taaaggagcg gtcaaaatgg
     2341 aatgttatct tgacaacagc gcaaccacaa agccctgtaa ggccgctgtg gacgcagaaa
     2401 tttccgcact gaccgagctt tacggaaatc cgtcttcgct tcatcaaaaa ggcgtttacg
     2461 catatatgct tctcgaaaaa agccgcggta cgattgccgc aagcttaaaa gcgagccccg
     2521 atgaaatata tttcaccccc tgcggcaccg tgtcaaacaa cacagcgata ttcggtgcag
     2581 tcgacgcaaa aaagcgactc ggcaagaaaa tagtcactac ggctttagag catccgagcg
     2641 ttgaaaaatg tatggaacgc ctcgaggaaa gcggctttga ggtagtgaga gtacagccgc
     2701 aaagcgacgg caatataagc ttaaaggacn nnnnnnnnnt cgaaaacgcc atagacgaaa
     2761 atacgatttt ggtatctgta atggccgtca acaacgaagt cggctctgtg atgcccacag
     2821 aaaatctcaa aaaaatcata aagagcaaaa attctcccgc tcttctgcac gttgacgatg
     2881 tgcagggcta tatgaaaatt ccgctctgtc ccaaaagctg cggaatagac cttatgagcg
     2941 tcagtgcaca caaggttcac ggtcccaagg gcgtgggagc gctttatata aataaaaacg
     3001 tgcgcataaa cccgtatatt ctcggcggcg gtcaggaaaa caatatgtgc tcgggaactc
     3061 agggtatgcc ggcgatagca ggctttgccg ccgctgttga aa
//
LOCUS       DBMDVO010000077         3081 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14485_length_3081_cov_1.542617, whole genome shotgun sequence.
ACCESSION   DBMDVO010000077 DBMDVO010000000
VERSION     DBMDVO010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14485_length_3081_cov_1.542617"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="ACZPE2_01840"
     CDS             complement(<1..188)
                     /locus_tag="ACZPE2_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIR1318621.1"
                     /translation="MRFILVSPKNRTAYNFRGDLIKRIISCGYEVIVTGPNRVDVEKI
                     EALGARFVEIPMNKNGVNP"
     gene            complement(185..>805)
                     /locus_tag="ACZPE2_01845"
     CDS             complement(185..>805)
                     /locus_tag="ACZPE2_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1318622.1"
                     /translation="EFAVLGTLLIYKKSFFFRENQKNHVWQKNRELFELCGKTVLTVG
                     CGSVGSECARRYKALGCRVLGVDLYPTENELYEKIYPLSVLPSILSVSDAVILTLPLT
                     DETRGLFDGEMLSHMKNTAVLINISRGAVVDEDALTAALSEKKLYGAVLDVFESEPLS
                     QDNPLWDMENVVVTPHNSFVSEKNSERLFSVIYGNLSDFLKGDKAE"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<816..1106)
                     /locus_tag="ACZPE2_01850"
     CDS             complement(<816..1106)
                     /locus_tag="ACZPE2_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1318623.1"
                     /translation="MKILLTGAYKYTEGQLSRLKALGADFLYVEDEREELSFDVSAVD
                     AVVCNGLFLYNDIKKFKNLKFIQLTSAGLDRVPAEYIKSKNISLFNARGVYSV"
     gene            complement(1314..1409)
                     /locus_tag="ACZPE2_01855"
                     /pseudo
     CDS             complement(1314..1409)
                     /locus_tag="ACZPE2_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(1545..1979)
                     /locus_tag="ACZPE2_01860"
                     /pseudo
     CDS             complement(1545..1979)
                     /locus_tag="ACZPE2_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(1979..2629)
                     /locus_tag="ACZPE2_01865"
     CDS             complement(1979..2629)
                     /locus_tag="ACZPE2_01865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1318624.1"
                     /translation="MRGFYRAVKRFLDVTASFLGLVLLSPLLLAVSILIKIDSRGPVI
                     FRQKRIGRNGKVFEIYKFRSMCVGAEKTGSGVYSGKGDARVTRIGKILRATSIDELPQ
                     LLNILKGEMSFVGPRPPLTYHPWKYEEYTDFQKRMFEVRPGITGWAQVNGRKDVEWHK
                     RIELNVWYVDHMSLLLDIKIMFMTAFKVLTNADNENSGATVNKSTESSNDAKVVKK"
     gene            complement(2788..>3081)
                     /locus_tag="ACZPE2_01870"
     CDS             complement(2788..>3081)
                     /locus_tag="ACZPE2_01870"
                     /inference="COORDINATES: protein motif:HMM:NF049034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR1318625.1"
                     /translation="SAVFELVRDINSNVIPTSSKELLIFAKELFSELTGVLGLVYEEK
                     DNDLDGKVEELIAARTAARKAKDFAKADSIRDEIAAMGIVLEDTPNGVKWHKK"
BASE COUNT          811 a          806 c          588 g          866 t
ORIGIN      
        1 ggatttactc cgtttttatt cataggtatc tcgacgaagc gtgcaccgag agcctcgatt
       61 ttttcaacat ccaccctgtt ggggcccgtg acaataacct cgtatccgca agagatgatt
      121 ctctttatta agtcgcctct gaaattatac gccgttctgt ttttcggcga aaccaatatg
      181 aacctcactc tgccttgtcc ccctttagga agtcgcttaa attaccgtat atcacggaaa
      241 acagccgctc cgaatttttc tccgaaacaa atgaattgtg cggagttaca acgacatttt
      301 ccatatccca taaaggattg tcctgcgaca gcggttcgct ttcaaaaacg tcgagcaccg
      361 ctccgtaaag cttcttctcc gacagtgccg ccgtaagggc gtcctcgtca accaccgcgc
      421 ccctcgaaat gttgataagc acggctgtat ttttcatatg agaaagcatt tctccgtcaa
      481 acagccctct cgtctcatcg gtaagcggaa gcgttaaaat gaccgcatcc gaaaccgaaa
      541 gtatactcgg aagaaccgac agcggataaa ttttttcgta aagctcattc tccgtcgggt
      601 acaaatcgac tcctaaaaca cggcagccta aagccttata tcttctcgca cattcgctgc
      661 ctacggagcc gcagccgact gtcaaaacgg tctttccgca aagctcaaaa agctccctgt
      721 ttttttgcca aacgtgattt ttttgatttt cgcggaagaa aaagctcttc ttataaatta
      781 acagagtacc gagaacggca aattcnnnnn nnnnncacac tgtaaacacc ccttgcgttg
      841 aaaagggaaa tatttttact ttttatatac tccgcaggga ctctgtcaag ccccgcgctt
      901 gtgagctgaa taaatttaag atttttgaat tttttgatgt cgttatataa aaacaggccg
      961 ttgcacacaa cggcgtcaac agccgaaacg tcaaaagaga gctcctctct ctcgtcctcg
     1021 acatataaaa agtccgcccc caaggcttta agccgtgaaa gctgaccttc cgtatattta
     1081 tatgcaccgg tcaaaaggat tttcattaaa agatgcccct gtaaatgatt ataaaacgaa
     1141 gtttttcaga ccttgcaagc cttttttgcc tcaactattg aattgacgca agccacaagc
     1201 ttttcgtgat tttccgcaag ctcgtccgag ctcatatttt tgacaactcc ctcatattca
     1261 cgaaactctt tgacgccgac attgaatctg tcaaagattt catcctcacg aagctttgaa
     1321 gcgtcgcaaa aggctctcga taatataacc ataccggagc cgagcctgta atgctcacct
     1381 attatgtatt ccgcaggcag cataacctcg ctgtgaactc tgcccacgcc gccgaagccg
     1441 taaaatatgc ccctttcgcg gagcttacgg cacagtcttt ccaccgtacc gtccgcaaca
     1501 agctggaaca taaagtccat tttatagccg agatgcaagt cgttaagccc tatatgcact
     1561 tcgtctatac cgggaacgtc aagaatttcg tctaagtgct ccgccgcctc gggcgtttcc
     1621 acaagaaggc atgtccttgc cctttttccg acgcagtctg taaagccttt tacctcttcc
     1681 gcggtcttaa aatacgggag cataactata tccgcaccgt cgtctattac gcggttgatt
     1741 tcttcacgtg agccgtcgta aaacgaattt actctgacca aaagctccgt cctgtctaaa
     1801 accgcgctga gctttttcac gtcgtcaatg ctgttatggg atattacggt atccatattt
     1861 gcctgacgct ctgccttacc gcgggtttca aggtcaagga atattcgatc taccgaaacg
     1921 tcctgtgcga tttttgcaat cttcgggtcg tttgtgagat acatcagctt aagagccatt
     1981 attttttcac aacctttgca tcatttgacg attcggtgct cttatttacg gttgcaccgc
     2041 tgttttcgtt gtcggcattc gtaagtacct taaacgcggt cataaacata attttgatgt
     2101 caagtaaaag cgacatatgg tcaacatacc aaacgttaag ctcgattctt ttgtgccact
     2161 ctacgtcctt tctgccgttg acctgcgccc agccggttat accgggtcgc acctcgaaca
     2221 tacgcttttg gaaatcggta tactcctcat atttccacgg atgatatgta agcggaggcc
     2281 tcggcccgac aaagctcatc tcgcccttta aaatatttaa aagctgcggc agctcatcta
     2341 tgctcgtagc tcgtaaaatt ttacctatcc ttgtaaccct ggcgtcgccc tttccggaat
     2401 aaacgccgga gccggttttt tccgctccga cgcacattga gcggaattta tatatctcaa
     2461 aaacctttcc gtttctgcct attctcttct gacggaaaat aacgggaccg cggctgtcga
     2521 ttttaatcag tatcgacacc gcaagcaaca gcggagacaa caaaacaagc cccaaaaatg
     2581 acgctgtgac atctaaaaat cttttgactg ccctataaaa gcctctcacc gttacacacc
     2641 ctaaaaaaca ctatgattta actaatttat tatatactta tacattaaat atgtcaatta
     2701 aaacaggcga ttgtaaacat ttgtaaaaaa gcagcggacg acacggtaaa aacgatgtcg
     2761 gtcgctgctt ttaaaatcac atcaatatta ttttttgtgc cattttacgc cgttcggagt
     2821 atcctccaaa actatgccca tggcggctat ctcatcacgg atgctgtccg ccttagcgaa
     2881 gtccttcgcc tttctcgcag cggttcttgc ggctatcagc tcttcaacct tgccgtcaag
     2941 gtcgttgtcc ttttcctcgt acacaagacc gagtacgccc gtaagctccg aaaacagttc
     3001 ttttgcaaat atcaaaagct ctttggacga agtgggtatt acatttgagt ttatatctct
     3061 gacaagctca aataccgctg a
//
LOCUS       DBMDVO010000078         3056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14677_length_3056_cov_1.058206, whole genome shotgun sequence.
ACCESSION   DBMDVO010000078 DBMDVO010000000
VERSION     DBMDVO010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14677_length_3056_cov_1.058206"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPE2_01875"
     CDS             complement(<1..572)
                     /locus_tag="ACZPE2_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR1318626.1"
                     /translation="MSKMSEVMPKWGPYSKKYSGVSRVTEHETEKGVRFDFISLPAVS
                     NTDAKAPNVTIPVGVHPWDVKSDYSFYSYRQDLEWKDVIYSDVSFTKLSDESVLVRTE
                     IFNNSELMQNCLVNYFSSLQFPFLTSYRVSLPNKSLMFDALDYSEFTYKTSRPWDNET
                     MDAMHKGEFFDDRFTSHRGLGDRDDNRYILP"
     gene            complement(742..1194)
                     /gene="rpiB"
                     /locus_tag="ACZPE2_01880"
     CDS             complement(742..1194)
                     /gene="rpiB"
                     /locus_tag="ACZPE2_01880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1318627.1"
                     /translation="MKIAIGSDHVGYELKGKVIAHLKEKGIEVKDFGTNSTERTDYPI
                     YGEAVANAVASKEFDKGILICGTGVGISLAANKVNGIRAVVCSEPYSALLSRQHNDTN
                     ILAFGARVVGLDLALMIVDTWLSGVYEGGRHARRVQMISDIEERQKNK"
     gene            complement(1311..2303)
                     /gene="alsB"
                     /locus_tag="ACZPE2_01885"
     CDS             complement(1311..2303)
                     /gene="alsB"
                     /locus_tag="ACZPE2_01885"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HIR1318628.1"
                     /translation="MKRKILSIVLALTVVIGIVFAGCGSKTDDKKTTDGSGSSSGTAE
                     YAIILKPLSNDFWANMKAGIEKEAKELGVTVDIFAAQSEDDTEGQLKILENCISKGYK
                     AIGVAPISPTNLINGIVEANKKGIYVMNIDEKIDMDTLKNAGGSVIAFATTDNEKVGE
                     KGANYIMEQLKDGGEVAIIEGKAGNASGEYRKNGATNAFTANSAFKLVDSQPADWDRQ
                     KALDTAASMIQKYPNLKAIYCCNDTMALGALQAVKNANKLGKIMVVGTDGAAEAIDSV
                     KAGELSATVAQDSAGIGAVSLKTMVETVKAGKAIDVNATPETIPVDSYIVTKDS"
     gene            complement(2349..>2789)
                     /locus_tag="ACZPE2_01890"
     CDS             complement(2349..>2789)
                     /locus_tag="ACZPE2_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1318629.1"
                     /translation="RNGAAGELGHIPLPNIEEKCTCGNVGCSEVICSGKYLEKITAEL
                     FPETDIKNVFFEHGDDPRIIKYVKDLAIPIATEVNILDPDYIIIAGGVVYMKNFPKDI
                     LIDSVHFHTRKPYPEKNLRILFSEHTQQSGVYGSGDFAWHQLKQ"
     assembly_gap    2790..2799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2800..3042)
                     /locus_tag="ACZPE2_01895"
     CDS             complement(<2800..3042)
                     /locus_tag="ACZPE2_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1318630.1"
                     /translation="MPSMVSKDKKTVISTPNLKGFDNIPFGDTLSKKLGIPVYVDRDV
                     NFLLQNDIVTLGLPKDSTVLGFYIGTGFGNAIYLNGS"
BASE COUNT          824 a          713 c          579 g          930 t
ORIGIN      
        1 ggcaatatat atcggttgtc gtcacggtct ccgagccctc tgtgcgaagt aaagcgatcg
       61 tcaaagaact cgcctttatg catggcgtcc atagtttcat tatcccacgg acggcttgtt
      121 ttatatgtaa actcgctgta atcaagcgcg tcaaacataa gcgatttatt gggaagagaa
      181 actctgtatg atgttaaaaa cggaaattgc agcgaagaga aataattgac aaggcaattt
      241 tgcataagct ctgaattatt gaaaatctct gtcctgacaa gcacactctc atcgctgagt
      301 tttgtaaaag aaacgtcgga ataaataaca tccttccact ccaggtcctg tctgtaagaa
      361 taaaaagaat aatcactttt tacatcccaa ggatgcacac ccacaggtat agtcacattc
      421 ggagccttgg catcggtatt tgaaactgcg ggaagcgaaa tgaaatcaaa cctgacgcct
      481 ttttccgttt cgtgctctgt gactcttgac acaccgctgt acttttttga ataaggcccc
      541 catttaggca ttacttctga cattttactc ataattctta catagccttt ccatttacag
      601 aataaaaacc aatcgctgca aacattacag ttccgtagaa aacacgctga tattgtatca
      661 gaaaaaaagc tctaattcaa gaaaaaaaga agtcgcaaaa cacagtttct gcggcttcct
      721 tttgtttaat taaataaaag cttatttatt tttctgtctt tcttcaatgt cggatatcat
      781 ttgcacgcga cgtgcgtgtc ttccgccctc ataaacaccg gagagccaag tgtcaacaat
      841 cataagtgca aggtcaaggc cgacaactct tgcaccgaaa gccaaaatat tggtatcgtt
      901 atgctgcctc gaaagaagtg ccgaatacgg ctcactgcaa actaccgctc tgataccgtt
      961 aaccttattt gccgcaagag aaataccaac acctgtaccg catataagta tacccttgtc
     1021 aaattccttt gatgctaccg cgtttgccac agcctcaccg taaatcgggt aatcggtacg
     1081 ctcggtgctg ttcgttccga aatcctttac ctctatacct ttttctttca ggtgagcaat
     1141 aaccttgcct ttaagttcgt atcctacatg gtcgctacct attgctattt tcatattaaa
     1201 acctctttca atattccgtt atacagctcg acagcaccga aaaatcggtg ctgtctgagc
     1261 ttgtgagtta gggagttcgt ttatctaata aacggatgga aaacaccttt ttagctatcc
     1321 tttgtaacta tataagaatc aaccgggata gtttcgggag tagcattaac atcaattgct
     1381 ttgcctgctt ttactgtctc aaccatcgtt ttaagtgaaa cagcaccgat tcccgctgag
     1441 tcctgagcaa ctgttgctga aagctcgcct gcttttacgc tgtctatggc ttcggctgca
     1501 ccgtctgtac ctactaccat tatcttaccg agcttgttag cgtttttaac tgcctgtaaa
     1561 gcaccgagag ccatggtatc gttgcagcaa tatatagcct taagattcgg atatttctga
     1621 atcattgaag cggcagtatc aagagccttc tgtctgtccc agtcagccgg ctgagaatca
     1681 acaagcttga atgcgctgtt tgcggtaaat gcatttgttg caccgttctt tctgtactca
     1741 ccggatgcgt tgcctgcctt accttcgatt attgcaacct cgccgccgtc ctttaactgt
     1801 tccattatat agtttgcgcc tttttcaccg actttttcgt tatcggttgt agcaaaagct
     1861 ataacacttc cgccggcatt cttaagggta tccatatcaa tcttttcatc aatattcatt
     1921 acataaatac cctttttgtt tgcctcaact ataccgttaa taaggttagt cggggaaatc
     1981 ggtgcaacac ctatagcctt gtagcccttg gagatgcagt tttcaagaat tttaagctga
     2041 ccctcggtat catcctcgga ttgcgcggcg aaaatgtcaa cggttacgcc gagttcttta
     2101 gcttccttct ctattccggc tttcatattt gcccagaaat cgttagaaag cggtttaaga
     2161 atgattgcgt attctgcggt tccggaagaa gagcctgaac cgtcggtcgt tttcttgtcg
     2221 tcggtcttac ttccgcaacc agcgaaaaca atgccgataa ctaccgtcaa tgccaaaacg
     2281 atacttaaaa ttttcctttt cataataaat tcctctctct tttttactta ttttttcagc
     2341 agtcgaaact actgtttaag ctgatgccat gcaaagtctc cactcccgta aactccgctc
     2401 tgttgtgtat gctccgaaaa aaggattctg agattttttt cggggtaagg ctttcgtgta
     2461 tgaaagtgta ccgaatctat caaaatgtcc ttcggaaaat tcttcatata taccactccg
     2521 ccggcgatta taatataatc gggatcaagt atgttgactt cggtagctat aggaatggca
     2581 agatctttaa catacttgat tattctcgga tcgtcgccgt gttcgaaaaa aacatttttt
     2641 atatcggttt cgggaaaaag ctccgcggta atcttttcaa gatacttgcc tgagcaaatt
     2701 acttcgctgc acccgacatt tccgcacgta cacttttcct caatattcgg caacggtatg
     2761 tgcccgagct ctcctgccgc tccgttgcgn nnnnnnnnng ctgccgttta aataaatggc
     2821 atttccaaag ccggttccga tataaaagcc gagaacagta gaatctttcg gaagacccaa
     2881 tgtaacaata tcattttgaa gaagaaaatt tacatcgcgg tcaacatata cgggtatgcc
     2941 aagctttttg ctgagcgtat ctccgaacgg tatattatca aatcccttaa gattaggagt
     3001 ggatataaca gtctttttat ccttgctgac catggacggc aatccaattg ctaccg
//
LOCUS       DBMDVO010000079         3052 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14707_length_3052_cov_1.316164, whole genome shotgun sequence.
ACCESSION   DBMDVO010000079 DBMDVO010000000
VERSION     DBMDVO010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14707_length_3052_cov_1.316164"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="ACZPE2_01900"
     CDS             complement(<1..876)
                     /locus_tag="ACZPE2_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1318631.1"
                     /translation="MENITFESIKIGLASPEQIREWSYGEVKKPETINYRTLKPERDG
                     LFCERIFGPTKDWECHCGKYKRIRYKGKICERCGVEVTKSKVRRERMGHIELVAPVSH
                     IWYFKGIPSRMGLILDISPRELEKVLYFAKYIVTDPGDTELKKKQILSEKEYLDMREK
                     YEDDFKAGMGAEAIKELLCEIDVDALTEQLREELSEASGQKKARILKRLEVAEAFKLS
                     GNRPEWMILDVIPVIPPDIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELNAP
                     DIIVRNEKRMLQEAVD"
     gene            complement(928..>3052)
                     /gene="rpoB"
                     /locus_tag="ACZPE2_01905"
     CDS             complement(928..>3052)
                     /gene="rpoB"
                     /locus_tag="ACZPE2_01905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1318632.1"
                     /translation="DVHYSHYGRMCPIETPEGPNIGLISYLATFARINKYGFIEAPFR
                     RIDKETGTVLDEVTYMTADVEDNYMVAQANEPLDENGKFKRKKVNARYRDEFMEIEAS
                     EADYMDVSPKMVVSVATAMIPFLENDDANRALMGSNMQRQAVPLLTTESPIVGTGMEY
                     KAAVDSGTVVLAKRAGTVTKADAETVEITTDDGSVDHYDIIKFMRSNQGTCINQRPIV
                     SEGQKVEAHEVIADGPATSNGEISLGKNALIGFTTWEGYNYEDAVLINEKLVRNDVYT
                     SIHIEEYELESRDTKLGPEDITRDIPNVGEDALSDLDENGIIRIGAEVHSGDILVGKV
                     TPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGEYGIVVNVEVFTRENSDELSPG
                     VNKVVRCYIAQKRKISVGDKMAGRHGNKGVVSRILPQEDMPFLDDGTPLDILLNPLGV
                     PSRMNIGQVLEVHLGFAYRKLGQKIATPVFDGAHESDIREALKEAGYREDGKSILTDG
                     RTGEKFDNPITVGVMYFLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEM
                     EVWALEAYGAAYTLQEILTVKSDDVVGRVKTYESIVKGLNVPKPGIPESFKVLIKELQ
                     SLALDVKVLDANNEEIDLKQTYDDDDVTPTMADNEAFSEVNDASYDDGYIPDREEDAD
                     EYENDDYTSDSEDGFDSDDLNDEDF"
BASE COUNT          706 a          777 c          715 g          854 t
ORIGIN      
        1 atcgacagcc tcctgaagca ttctcttttc gtttcttaca attatatcgg gagcgttgag
       61 ctcaagaagt cttttaagac ggttgtttct gtttataaca cgtctgtaaa gatcgttgag
      121 gtccgatgtt gcaaatctgc cgccgtcgag ctgtaccata ggacggatat cgggcggaat
      181 taccggaata acgtcgagaa tcatccattc gggacggtta ccggaaagct tgaatgcttc
      241 ggcgacttca agtcttttaa gtattcttgc tttcttctga cccgacgcct cggagagctc
      301 ctcacggagc tgctcggtaa gcgcgtcaac gtcgatttcg cagagaagct ctttgatggc
      361 ttcggcgccc ataccggcct tgaagtcatc ctcatacttc tctctcatat cgagatactc
      421 tttttcggag agtatctgct tctttttaag ctctgtatca ccgggatcgg tgacaatata
      481 ttttgcgaaa tagagaacct tttcaagctc tctcggagaa atgtcaagga taagtcccat
      541 tcttgacggg atacccttga aataccaaat atgcgaaacg ggagcgacaa gctcgatatg
      601 gcccattctc tcgcgtctta ccttggattt ggtaacctca actccgcagc gctcgcatat
      661 tttaccctta tagcgaattc tcttgtattt tccgcaatgg cactcccagt cctttgtggg
      721 tccgaatatc ctttcgcaga aaagtccgtc gcgctcgggc tttaaggtac gataatttat
      781 ggtttcgggc tttttgacct cgccgtaaga ccactctctg atctgttcgg gtgaagcaag
      841 accgattttt attgattcaa aggttatatt ttccattggc tctttacctt tcttccatta
      901 attttcagaa atctgcgaaa cgtgaaatca gaaatcctcg tcattgaggt catccgaatc
      961 aaaaccgtct tcggaatcgc ttgtataatc gtcattctcg tattcgtcgg cgtcttcttc
     1021 tctgtcagga atataaccgt catcataaga agcgtcgttg acctctgaaa atgcttcgtt
     1081 gtcagccatc gtaggagtaa catcatcgtc gtcgtaggtc tgtttaaggt caatctcctc
     1141 gttatttgcg tcaagaacct tgacgtcaag agcaagcgac tgaagctcct taatgagaac
     1201 cttgaacgac tccggaatac cgggcttcgg aacgttgaga cccttgacta tcgactcgta
     1261 agtcttcaca cggcccacaa catcgtccga cttaacggtg agaatttcct gaagagtata
     1321 agctgcgccg taagcctcaa gggcccaaac ctccatctca ccgaaacgct gaccgccgaa
     1381 ctgagcttta ccgcccaagg gctgctgggt aacgagtgaa tacggacccg tgcttcttgc
     1441 gtgaatctta tcgtcaacaa gatggtggag cttaaggaag tacataacgc ctacggttat
     1501 ggggttgtca aacttttcac ccgttctgcc gtcggtaagt atcgacttac cgtcctcacg
     1561 gtagcctgcc tctttaagag cctcgcgaat atcggattca tgcgcaccgt caaataccgg
     1621 cgttgcgatt ttttgaccga gcttgcggta agcaaagccg aggtgaacct caagcacctg
     1681 accgatgttc attcgggagg gtacgccgag ggggttaaga agaatgtcaa gcggagtacc
     1741 gtcatcaagg aacggcatat cctcctgcgg aagtattctt gaaacaacac ccttgttacc
     1801 gtgacggcct gccatcttat cgccgacgga aatcttacgt ttctgagcga tgtagcagcg
     1861 tactacttta ttaacgccgg ggctgagctc gtcgctgttt tctcttgtaa acacttcgac
     1921 gtttactact atgccgtatt cgccgtgcgg tacgcgaagg gaagtatcgc gaacctctct
     1981 tgccttttcg ccgaatattg cgcggagaag tctctcctcg gcggtaagct ccgtctcgcc
     2041 cttaggcgtt accttaccga caagtatatc gcccgagtga acctcggcac cgatacggat
     2101 tatgccgttt tcgtcaaggt cggaaagagc gtcttcaccg acgttcggga tatctctcgt
     2161 aatatcttcc ggtccgagct tggtatctct cgactcaagc tcatattcct ctatatgaat
     2221 tgaagtataa acatcgttgc ggacaagctt ttcgttgata agaacagcat cctcgtagtt
     2281 gtagccttcc caagtggtaa agccgataag cgcattctta ccgagcgaaa tttcgccgtt
     2341 tgaggttgca ggaccgtcgg ctataacctc atgagcctca accttttgac cctcggaaac
     2401 tatcgggcgc tgatttatgc atgtgccctg gttagaacgc ataaatttga ttatgtcata
     2461 atggtcaacc gaaccgtcat ccgtagtgat ttcaacagtt tccgcatcag ccttggttac
     2521 ggtaccggcg cgctttgcga gaactacggt gcccgaatcg accgccgcct tgtattccat
     2581 accggttcct actataggag actcggttgt gagaagcgga accgcctgtc tctgcatgtt
     2641 tgaacccata agtgcacggt ttgcgtcgtc gttctcaagg aaaggaatca ttgcggttgc
     2701 aacggaaact accatcttcg gcgatacgtc catgtagtcg gcttccgaag cctcaatctc
     2761 cataaactcg tcacgataac gggcattgac ctttttgcgc ttgaatttgc cgttttcgtc
     2821 gagaggctcg ttagcctgag ctaccatgta gttatcctcg acgtctgcgg tcatataggt
     2881 gacctcatca agtaccgttc ccgtttcctt atctatgcgg cggaacggag cctcgataaa
     2941 gccgtattta ttgattcttg caaaggttgc aagataagag ataagtccga tgttcggtcc
     3001 ttcgggggtc tctatggggc acatacggcc gtagtggctg taatgaacgt ca
//
LOCUS       DBMDVO010000080         3043 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14790_length_3043_cov_1.245411, whole genome shotgun sequence.
ACCESSION   DBMDVO010000080 DBMDVO010000000
VERSION     DBMDVO010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14790_length_3043_cov_1.245411"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="ACZPE2_01910"
     CDS             complement(<1..1446)
                     /locus_tag="ACZPE2_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318633.1"
                     /translation="MADRNMSSGFSLDDILSETEKDTEKVPGKLWSLAEIDALLADEG
                     LSESEKVQTEKAHEPKEQTEHAEKKYESAEEKNGKTPVLHTSKKEPDKKSDDSFSIDP
                     AIFDEILSGGSRSEKTEDRKINAAKIEEDKKAFGKDDDSKKAAGVNNASSEILPENPL
                     FGGTTPSLTSDNENSPVQSENESVPGQISLEKTRVFNEVDARAIRSDKIEHHIGKKIT
                     RTTSSEFTDKKPAVKKGMETDKYRERFLNKPKLDMEKTREHEKLMSSLPPKTIEKFGV
                     IVKKPNGEKTSEEGLMPVPTIISAEDELREQKSNAEKYRTGELKSLAKKNAADGDTQQ
                     LQNQIKLDGFEEEVKPETVDEYEAEIELLGRRSKKAKSFKLQPAFRQTEKEEVHRSEP
                     EDDESDEENKTAEKIRKNIRILDDLPEEDENGDVIDESAECDDDGVDLEPKKKQRRSK
                     REKSDNGDEPIRIAREYYSNKDAKAVYDIMMG"
     gene            complement(1464..2210)
                     /gene="rlmB"
                     /locus_tag="ACZPE2_01915"
     CDS             complement(1464..2210)
                     /gene="rlmB"
                     /locus_tag="ACZPE2_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1318634.1"
                     /translation="MDEARNDQNDLIIGRNAVFEAIKSGREIDRLFVQRGEQNPAVKR
                     LISMAREKGAVIKEVDSKKLDFMCGGGVHQGVALSAAAHEYSTVEDILNKAAEKNEKP
                     FIIICDGVEDTHNLGAVIRTADAAGAHGVIIPKRRSASLNFTVGKTSAGALEYVPVAR
                     VANLASTIDELKEKNIWIYGADMDGSDYRSVDYSGGVALVIGSEGRGMSRLVREKCDF
                     IVSLPMKGKINSLNASVAAGILMYEAASHR"
     gene            complement(2509..>2708)
                     /locus_tag="ACZPE2_01920"
     CDS             complement(2509..>2708)
                     /locus_tag="ACZPE2_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR1318635.1"
                     /translation="GARRGDAAEMCIIELVETKAELEAKAAAAKKAAKKPAAKKPAAK
                     KSAPKAEKKEAETDATAEAKE"
     assembly_gap    2709..2718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2719..2999)
                     /gene="rplQ"
                     /locus_tag="ACZPE2_01925"
     CDS             complement(<2719..2999)
                     /gene="rplQ"
                     /locus_tag="ACZPE2_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR1318636.1"
                     /translation="MPGTRKLGRTTDHRKAMLRGMVTYLFENGKVETTVTRAKEVRAM
                     AEKMITIAKDNSLHARRQVLAYVTKEDVVKNLFDVIAPRYADVNGGYTR"
BASE COUNT          629 a          783 c          624 g          997 t
ORIGIN      
        1 gcccatcatt atatcataga cggcctttgc atccttgtta ctgtaatatt cgcgcgctat
       61 ccttatcggc tcgtcgccgt tatctgattt ttcgcgcttg ctgcgtcgct gctttttctt
      121 cggctcaaga tctacgccgt cgtcatcgca ttcggcggat tcgtctatga catctccgtt
      181 ttcgtcctcc tcggggaggt cgtcgagaat gcgaatattt tttcttatct tttccgcagt
      241 tttgttttct tcgtccgact catcgtcctc aggttcgctg cggtgaactt cctctttttc
      301 ggtctgtctg aacgcaggct gcaatttaaa gctcttggct tttttgcttc gcctgcccaa
      361 aagctctatt tcagcctcat attcgtcaac tgtttcaggc ttaacctctt cctcaaagcc
      421 gtcaagcttt atctgatttt gcagctgctg tgtatcgccg tccgcagcat tctttttggc
      481 gagggactta agctcgccgg tgcggtattt ttcggcgttg cttttttgtt ccctcagctc
      541 atcctctgcc gagattattg tgggaacggg catcaagccc tcctcactcg tcttttcgcc
      601 gttgggcttt tttactataa ccccgaactt ttcaatggtt ttgggcggga gcgacgacat
      661 aagtttttcg tgctcgcgtg ttttttccat atcaagtttc ggtttattaa gaaacctctc
      721 gcggtattta tccgtctcca ttcccttttt gacagcgggt tttttgtcgg taaattccga
      781 agatgttgtt ctggttattt ttttgcctat atgatgctct attttatcgc tgcgtatggc
      841 tcttgcatca acctcattga aaacgcgcgt cttttcgagc gaaatctgcc ccggtaccga
      901 ttcgttttcc gactgcacgg gcgaattttc attatcgctt gtaaggcttg gggttgtccc
      961 tccgaaaagc ggattctcgg ggagaatctc ggaagaagca ttatttactc cggccgcttt
     1021 tttcgagtca tcgtctttgc cgaaagcttt cttatcttct tcaattttcg cggcattgat
     1081 ttttctgtcc tcggtttttt cgctgcggct gccgcccgat aaaatctcgt caaaaatggc
     1141 ggggtctatg gaaaaactgt cgtccgactt tttgtcgggc tctttttttg atgtgtgtaa
     1201 aaccggtgtc ttgccgtttt tttcttcggc tgactcatat tttttctctg cgtgttccgt
     1261 ctgctctttc ggctcgtgag ccttctccgt ttgaaccttt tcggactcgg acagcccctc
     1321 atcagctaaa agggcatcaa tctccgcaag cgaccagagc ttgcccggca ctttttctgt
     1381 atctttttcg gtttcgctga gaatatcgtc aagagaaaat cctgacgaca tattacggtc
     1441 tgccattttt cacaccccaa tttttatctg tgcgacgccg cctcatacat aaggatgcct
     1501 gccgccacag aagcattcaa tgagtttatt tttcccttca tgggaagcga gacgataaaa
     1561 tcgcattttt cgcggacaag acggctcata ccgcgaccct cggaaccgat aaccaaagcg
     1621 actccgcctg aataatccac agacctgtaa tcgctgccgt ccatatcggc accgtatatc
     1681 caaatatttt tttccttaag ctcatcaatg gttgacgcaa gatttgcaac cctcgcaacc
     1741 ggcacatatt cgagtgcgcc tgccgaggtt ttacccacgg taaaattcag agaagcactg
     1801 cggcgcttcg gtataatcac gccgtgtgcg cctgccgcat cggcggttct tattactgca
     1861 ccgagattat gcgtgtcctc tacgccgtca caaattatta taaacggctt ctcatttttc
     1921 tcggccgctt tatttaaaat atcttcaacg gtagagtatt catgcgccgc cgccgaaaga
     1981 gccacaccct ggtgcactcc tccgccgcac ataaaatcga gcttttttga gtcaacctct
     2041 ttaatgactg cgcctttttc gcgagccatt gagattaacc ttttaacagc agggttttgc
     2101 tcgccgcgtt gaacaaacag gcggtcaatc tcacggcctg atttgattgc ctcaaatacc
     2161 gcgtttctgc ctataattaa atcgttctgg tcatttcttg cttcgtccat caaaatactc
     2221 tccggtaaac gtcaataaat tatatatatt atattgtaac ataactcagc ctgtttttac
     2281 aatagaatta tcaagtttaa acctcaccga accaaaaaaa gccctcgatt ttaataaccg
     2341 caaaaaaatc agcgcggttt tccatcaaaa acgggcttta ctgtttcgga gtcgataatt
     2401 tgaatgcaga aaaaaggctt tgcttttcag acgcattcaa atccaaaagg gattgcaaaa
     2461 aacataagtt tttcacaacc cctttgcgaa taattatatt gctatatatt attctttagc
     2521 ttcggccgta gcgtcggtct cggcttcttt tttctctgcc ttgggagcag actttttagc
     2581 tgcgggcttt tttgctgcgg gcttctttgc agccttttta gctgcggcag ccttagcttc
     2641 aagctcagcc ttagtctcaa caagctctat aatgcacatc tcggctgcgt cgccgcggcg
     2701 tgcaccggnn nnnnnnnnat tcttgtatat ccgccgttaa catctgcgta tctgggcgca
     2761 ataacgtcga agagattttt aacaacgtcc tctttggtaa cgtaagctaa aacctgtctt
     2821 cttgcgtgta aggagttatc cttagcaatg gtgatcattt tctctgccat tgctctaact
     2881 tccttagccc ttgtaacggt cgtttcgact tttccgtttt caaacaggta agttaccatt
     2941 ccgcgaagca tagccttgcg gtgatcggta gtcctgccga gttttcttgt tccgggcatc
     3001 tataacactc cttctgatta ttcgtcatcc ttgcggagag tga
//
LOCUS       DBMDVO010000081         3038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14839_length_3038_cov_1.279196, whole genome shotgun sequence.
ACCESSION   DBMDVO010000081 DBMDVO010000000
VERSION     DBMDVO010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14839_length_3038_cov_1.279196"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="gmk"
                     /locus_tag="ACZPE2_01930"
     CDS             <1..511
                     /gene="gmk"
                     /locus_tag="ACZPE2_01930"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1318637.1"
                     /translation="DTLLDILLRKDPTLQKSISITTRLKRENEHDGVDYYFITPDDFE
                     AMLESGDVLEFAKYGKNLYGTPKKPVDKWLSEGKTVILKIEVQGAAKIKKMYPLSTAI
                     FIMPPSLEILEHRLRCRGTEDEDDLVRRLEIARGEIEKSRGYDYIVINDDLESAADEV
                     LDIIEKIDK"
     gene            523..732
                     /gene="rpoZ"
                     /locus_tag="ACZPE2_01935"
     CDS             523..732
                     /gene="rpoZ"
                     /locus_tag="ACZPE2_01935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR1318638.1"
                     /translation="MFNPDLKNILKDHLSRYSLVTATAKRAREISEKAEEDGEIMIEK
                     PVSIALDEILSGKYKIIEPEEIRYI"
     gene            756..>909
                     /locus_tag="ACZPE2_01940"
     CDS             756..>909
                     /locus_tag="ACZPE2_01940"
                     /inference="COORDINATES: protein motif:HMM:NF036997.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR1318639.1"
                     /translation="MIAKVAVENTAFSFDDAFDYAIPEKLSKDVRPGVRVLVPFGRGS
                     KKRQGVV"
     assembly_gap    910..919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <920..>3038
                     /gene="priA"
                     /locus_tag="ACZPE2_01945"
     CDS             <920..>3038
                     /gene="priA"
                     /locus_tag="ACZPE2_01945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1318640.1"
                     /translation="MLRLAVFMKDEYFCTLYEAVKAMLPTGLGLNLVTSYIVNPDVTD
                     DMLTSLSPDEKSVFSFLADKSVYVRGDRLLKELGFKQDFPIAEKMADKGFLIRNIDSV
                     RRIGDATVKMVKLSEFYYALREPPKFTAKQKQVVKFLTDTGSASVKEVCYFTGVTTAV
                     VTTLERKGILEFFDNEVFRRPKFLHGANKENIVLTSEQSAAFKDLCGRFQSDKAETSL
                     LFGVTGSGKTKVYMRLIDEVLKTGKGCIVMVPEISLTPQLLSLFYNRYGEKVAVFHSA
                     LSMGERLDEWKRVKNGEAKIALGTRSAVFAPFDNLGLVIMDEEQEGTYKSEMSPRYHA
                     KDVAAFRVGCHNGLLLLASATPSLTTYAAAKSGIYSLNVLKNRYGNAVLPEVITTEMK
                     YGADPTKTKNLSDELTLSIKETLENKKQAILLLNRRGYNTFAACESCGKVMTCPNCSI
                     SLTYHSKNGRLMCHYCGYSEPFTNVCRECGEKSVVFSGTGTQRLEEELHDSFPEARVL
                     RLDADTTTSRYSFENSLKKFTNGEYDIMLGTQMVAKGLDFPNVTLVGIISIDQQLYND
                     DYKSAERTFDLLTQVIGRAGRGDSKGRAVIQTSFPENEIIRLAEKQDFEAFYNLEISM
                     RKAMIYPPYCNLCVVSFTGSDEMLTKSASKVFLNMLKERQKNDFADLSIIVLGPIAPR
                     VSKISGKYRFRIIIKCKNTKKFR"
BASE COUNT          907 a          640 c          724 g          757 t
ORIGIN      
        1 ggacacgctc cttgacattc ttttgagaaa ggacccgacg ctccaaaaat caatttcgat
       61 aacaacgcgt ttaaaacgcg aaaacgagca tgacggggtt gattattact ttataactcc
      121 cgacgatttc gaggcaatgc tcgaaagcgg cgacgtgctt gaatttgcaa aatacggcaa
      181 aaacctttac ggcacgccaa aaaaaccggt cgacaaatgg ctgtccgagg gtaaaacggt
      241 tattttaaaa atcgaagttc agggtgcagc aaaaatcaaa aaaatgtatc ctctgtcaac
      301 agcgattttt ataatgccgc cgtcgcttga aatacttgag cacaggcttc gctgccgcgg
      361 tacggaggac gaggacgacc ttgtaaggcg ccttgaaatt gcgcgcggcg aaatagaaaa
      421 gagccgcggg tacgattaca ttgttataaa cgacgacctt gaaagcgccg ccgatgaagt
      481 tttggatatt atagaaaaaa tagataagtg aggttttatt ttatgttcaa tccggattta
      541 aaaaacattt taaaagacca tctcagccgt tattctcttg ttacagccac ggcaaaaaga
      601 gcaagagaaa tttcggaaaa ggccgaggaa gacggcgaaa taatgattga aaagcccgtc
      661 agcatagctc ttgacgagat actctccggc aaatataaaa taatcgagcc ggaagagata
      721 agatatatat aagtttattt taaggggtgc agcgtatgat agcaaaggta gcggttgaaa
      781 ataccgcttt cagctttgat gacgcttttg actatgctat acccgaaaaa ctctcaaaag
      841 acgtgcgtcc cggtgttcgt gtactcgtcc ctttcggcag gggcagtaag aaacgccagg
      901 gcgtcgtatn nnnnnnnnna gatgctgcga cttgccgtat ttatgaagga cgagtatttc
      961 tgcacgctgt acgaggcggt aaaggctatg ctgcctaccg gcctcgggct taatctcgta
     1021 acatcatata tagtaaatcc cgatgttacc gacgatatgc tgacttctct gtcaccggac
     1081 gaaaaaagcg tgttttcgtt tcttgcggac aagagcgtat atgtccgcgg cgacagactt
     1141 ctcaaagagc tcggatttaa acaggatttt ccaattgccg aaaagatggc agataaaggg
     1201 tttcttatca gaaatatcga ctccgtgcgg cgtataggtg acgccaccgt gaaaatggtt
     1261 aagttgtcgg agttttatta cgccctcaga gaaccgccta aatttaccgc taagcaaaag
     1321 caggttgtaa agttcctcac ggatacgggt tctgcttcgg taaaggaggt ttgctatttc
     1381 accggtgtta caacagccgt tgtgacaacg cttgagcgca aaggcatatt ggaatttttt
     1441 gataacgagg ttttccgccg cccaaaattt ttgcacgggg caaataaaga gaatatcgtg
     1501 ctgacaagtg agcaatcggc agcattcaaa gacctctgcg gtagatttca aagcgacaag
     1561 gccgaaacct cgctgctttt cggagttacg ggctccggga agacaaaagt gtatatgcgc
     1621 cttattgacg aggtgctgaa aacgggtaag ggctgtatag ttatggttcc cgaaatctct
     1681 ctcactccgc agcttctgtc tcttttttac aaccgttacg gtgaaaaagt ggcggttttc
     1741 catagtgccc tttcaatggg agaaagactt gacgagtgga aacgcgtaaa aaacggcgaa
     1801 gccaaaatag cgctcggtac gcgctcggcg gtttttgccc cgtttgacaa tctcggattg
     1861 gttattatgg acgaagagca ggagggtaca tataaatcgg agatgtcgcc gcgctatcat
     1921 gccaaggatg tggctgcatt ccgcgtagga tgccataacg gacttttgct tcttgcctcg
     1981 gcaaccccct cgcttacgac atatgcggcg gcaaagagcg gtatttattc tttgaatgta
     2041 cttaaaaacc ggtacggcaa cgcagttttg cccgaagtta taacaaccga gatgaaatac
     2101 ggagccgatc cgacaaaaac caaaaattta agcgacgaat tgactctttc gatcaaagaa
     2161 actctcgaaa ataaaaaaca ggcaatattg ctgcttaacc gccgcggcta caatacattt
     2221 gccgcgtgtg aaagctgtgg aaaggtgatg acctgtccga attgcagtat ctctcttaca
     2281 tatcacagca aaaacggcag attgatgtgc cattattgcg gatattcaga gccttttaca
     2341 aacgtttgtc gcgaatgcgg cgaaaaaagc gtggtatttt ccggaacggg aacgcaaaga
     2401 cttgaagagg aacttcacga cagctttccc gaggcgcgtg tgcttcgtct tgatgcggat
     2461 acaacaactt cgcgatattc ttttgaaaat tcactaaaaa agttcacaaa cggcgagtat
     2521 gatataatgt taggaacaca aatggtcgca aaaggacttg acttccccaa tgttacgctt
     2581 gtcggtataa tatctataga tcagcagctt tacaatgatg attataaaag tgccgagcgc
     2641 acctttgacc ttttaactca ggtcataggc agagcagggc gaggcgatag taagggcaga
     2701 gccgttatcc aaacctcttt ccccgaaaat gaaataatac ggcttgccga aaagcaggat
     2761 tttgaagcgt tctacaattt ggaaatatca atgcgtaagg ccatgattta tcctccgtat
     2821 tgtaacttgt gcgtggtgtc gtttaccgga agcgatgaga tgctcacaaa atcggcgtca
     2881 aaggtatttt tgaatatgct caaggagcgg caaaagaatg atttcgcaga tttaagcatc
     2941 atcgttttgg gacctatcgc tccgcgcgtc tcaaaaatat ccggcaaata cagattccgt
     3001 ataataataa aatgcaaaaa cacaaaaaaa ttcagatt
//
LOCUS       DBMDVO010000082         3029 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_14903_length_3029_cov_1.290984, whole genome shotgun sequence.
ACCESSION   DBMDVO010000082 DBMDVO010000000
VERSION     DBMDVO010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14903_length_3029_cov_1.290984"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="ACZPE2_01950"
     CDS             <1..273
                     /locus_tag="ACZPE2_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase beta subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1318641.1"
                     /translation="SNQLLATYAKVQDAKSLASVIGEDELSPDDKLLMKFGKEFDNRF
                     LNQSFTENRTIDETLDLGWELLSMLPRRMLDRVDDKTLDKYYKGKN"
     gene            307..933
                     /locus_tag="ACZPE2_01955"
     CDS             307..933
                     /locus_tag="ACZPE2_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1318642.1"
                     /translation="MAQVFPTKANLMATQKSLDLAKLGYELMDRKRNILVREMMQLIS
                     EAKEVEANINAVYDKAYSSLKLANITLGVCGNFARTIPVDNGLSLDFRSVMGVEIPKI
                     SLESSKLMPYFGFNSTNSHLDEAYINFNEAKKMTVKLAETEASIYRLADAVKKTQKRA
                     NALNNIMIPQFTGTIKYISEALEEKEREDFTRLKAVKNQKEEKKREAK"
     gene            complement(1117..2175)
                     /locus_tag="ACZPE2_01960"
     CDS             complement(1117..2175)
                     /locus_tag="ACZPE2_01960"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1318643.1"
                     /translation="MNKNSNYERSNTMAKKNIDWGSIGFSYIPTDKRYVANFKDGKWD
                     GGLTSDPNVVINECAGVLQYSQSCFEGLKAYTTEDGKTVCFRPDLNAARMKSSCERLK
                     MPVFPEDKFVEAVKEVVKANIDYVPPYGSGATLYIRPYMFGSNPVIGVKPADEYQFRI
                     FVTPVGPYFKGGAKPITIRVTDLDRAAPRGTGHIKAGLNYAMSLYNIVDAHENGFDEN
                     LYVDPATRTKVEETGGANCLFVDKNGTVVVPKSPSILPSITRRSLVTVAKDYLGLNVE
                     EREVYLSELSDFAEMGLCGTAAVISPVGKVYDHGKEICFPSGMDEMGPTIKKLYDTLT
                     GIQMGRIEAPEGWIVEIK"
     gene            2398..2850
                     /locus_tag="ACZPE2_01965"
     CDS             2398..2850
                     /locus_tag="ACZPE2_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1318644.1"
                     /translation="MSVYTERAAELFGKGFNCSQAVFAAFCDKFGMDEKTALKVASGL
                     GGGVGRMREVCGAMCGAAMVAGMVYGDDTGENREAKSYTYKKVQEIAAEFKKTNPSII
                     CRELLGLSKNCAVTPNAEKRTAEYYKKRPCAQIVEDAVKATEKVLFSE"
BASE COUNT          872 a          638 c          715 g          804 t
ORIGIN      
        1 tcaaaccagc tccttgcgac atatgccaag gttcaggatg ctaaatcgct cgcgtctgta
       61 ataggtgaag atgagctgtc gccggacgat aagcttctta tgaagttcgg caaggaattt
      121 gacaacaggt tcttgaatca gagctttacc gaaaaccgta ccattgacga gacgcttgac
      181 ctcggctggg agctcctttc aatgcttccg cgccgtatgc tcgaccgtgt tgatgataag
      241 actcttgata agtactataa aggcaaaaac tgagcctttt aaaattcgga aaaggaggaa
      301 aagccgatgg cacaggtgtt cccgacaaaa gccaatttga tggcgacgca gaaatctctc
      361 gatttagcca agctcggcta tgagctgatg gacagaaaac gtaacattct tgttcgtgag
      421 atgatgcagc ttataagcga agcaaaggaa gtcgaggcaa atataaacgc ggtatacgac
      481 aaggcttatt cgtccttgaa gcttgccaat attacgcttg gcgtttgcgg taattttgca
      541 agaaccatac ccgttgataa cgggctttcc cttgattttc gcagcgtaat gggtgttgag
      601 ataccgaaaa tatcgcttga aagcagcaag ctgatgccgt atttcggctt taattcgaca
      661 aactcccacc ttgacgaggc ttacataaac ttcaacgagg caaaaaaaat gaccgtaaag
      721 cttgccgaaa ccgaggcttc gatttaccgg cttgcggacg cagtcaaaaa aacgcaaaag
      781 cgcgccaacg cgttaaacaa cataatgata cctcagttta cgggtacgat taagtacatc
      841 tccgaagcac tcgaagaaaa agagcgtgaa gattttacaa ggcttaaagc cgttaaaaat
      901 caaaaagagg agaaaaaacg cgaggcaaaa taaaaatcag aggactgtaa aagctcgtct
      961 tttacagtcc tttttatgtg cttttttgcg gattatgctt ttgcggttgt tttttcttat
     1021 ccttattgag gattatattc agtgaccgaa tcgtgagcaa acataaaaaa tgctgtggaa
     1081 cgtatgttcc acagcatttt ttatatctgt ataagcttat tttatttcaa ctatccagcc
     1141 ttcgggagct tcgattctgc ccatctgaat tccggtgagg gtatcgtaaa gcttcttgat
     1201 ggtcggaccc atctcgtcca tacccgacgg gaagcaaatt tccttgccgt ggtcatatac
     1261 cttgcccacg ggagatataa cggcagcggt tccgcaaaga cccatctctg caaaatcgga
     1321 aagctcgctg aggtaaactt ctctttcctc aacattgagg ccgaggtaat cttttgcaac
     1381 tgttacaagc gaacgcctgg ttatagacgg aagaattgac gggctcttcg gaacgactac
     1441 ggtgccgttc ttatcaacaa agaggcagtt ggcgccgccg gtttcctcaa cctttgttct
     1501 tgtagcggga tcaacataga gattttcatc aaagccgttt tcgtgtgcgt caacaatgtt
     1561 gtagagcgac attgcgtagt taagacctgc tttgatatgg ccggttcctc tgggagctgc
     1621 gcggtcaagg tcggttacac gaatggtaat gggctttgca ccgcctttaa aataaggacc
     1681 taccggagtg acaaatatac ggaactgata ttcgtcggca ggcttaacgc cgataaccgg
     1741 atttgagccg aacatatacg gccttatgta aagggttgcg cccgaaccgt atgggggaac
     1801 atagtcgata tttgctttga ctacttcttt gacagcctct acgaatttat cttcgggaaa
     1861 tacgggcatt ttaaggcgct cgcatgatga tttcattctt gcggcgttga ggtcggggcg
     1921 gaagcatacc gttttgccgt cttccgttgt gtacgcctta agaccctcaa agcaagactg
     1981 cgagtactga agaacacctg cacactcatt gataacgacg ttgggatcag aggtaagacc
     2041 gccatcccat ttgccgtcct taaagttggc aacatatctt ttgtccgtgg ggatatagct
     2101 gaagcctatc gaaccccagt caatattttt cttcgccata gtattacttc tttcgtaatt
     2161 tgaattttta ttcattatat cacatatttt ctgttgtttg caatagaata tgtgagcttt
     2221 aacgcggcgt tttgacgaaa tatcggccta tttttgccat cggtttataa aaaatgttgt
     2281 atataaatgt cgcttatgtt aaaataatat aaaatgtgta taaaatacat aagtgccaat
     2341 ccgatagggt tcggcgctgc gtttaaaaca acaaaactca aaacggggtg ataatttatg
     2401 tcggtttata cagagcgcgc cgcggagctt ttcggcaaag gctttaactg ctctcaggct
     2461 gtttttgcgg cgttttgcga taaattcgga atggacgaaa aaaccgctct caaggtcgcg
     2521 tccggcctcg gcggcggagt gggcagaatg agagaggtct gcggagctat gtgcggagcc
     2581 gctatggttg caggtatggt atacggtgac gatacaggcg aaaacaggga ggcaaaatcc
     2641 tatacatata aaaaggttca ggaaattgcc gctgaattta aaaagaccaa tccgtctata
     2701 atctgccgcg aacttttggg attgtcaaaa aactgcgccg ttactccgaa tgccgaaaaa
     2761 cgcacggcgg agtattataa aaaacggcct tgcgctcaaa tagtggaaga tgcggtgaaa
     2821 gctacggaaa aagtgctttt ctcggaatag tgctccataa aagctcaact gcaaaatgaa
     2881 aaaaggaact gttttatgaa taaaaatacc gcatttgtct cttccgatga agagataata
     2941 tatataggaa acgaaaatac ggtgcgcgaa atatcgacct ccgataaggt attgcgaacc
     3001 gtccgcataa ttaacaggcg aatggacga
//
LOCUS       DBMDVO010000083         2992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15176_length_2992_cov_1.213767, whole genome shotgun sequence.
ACCESSION   DBMDVO010000083 DBMDVO010000000
VERSION     DBMDVO010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15176_length_2992_cov_1.213767"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="ACZPE2_01970"
     CDS             complement(<1..333)
                     /locus_tag="ACZPE2_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013942.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1318645.1"
                     /translation="MKKVLVLFGGVSSEHDVSCVSASYVVSNIPRDKYEVCPLGITKE
                     GEWFLFEGDVSLLPEDKWIKSGKCTKALLSPDRKDHGITVFRESGPEKIHIDVCFPVM
                     HGKNGEDGT"
     gene            668..1165
                     /gene="infC"
                     /locus_tag="ACZPE2_01975"
     CDS             668..1165
                     /gene="infC"
                     /locus_tag="ACZPE2_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1318646.1"
                     /translation="MQINEDIRDREVRVVTDGGEQLGIMSAKEALKKAEERNLDLVKI
                     SPNAKPPVCKIMDYGKYRFEQSKREKENRKNQRVIEIKEIRLSLNIDTHDFETKVNHA
                     HKFLKAGNKVKVSIRFRGREMAHPQIGEVSMKRFAEACEEFATVEKPAKLEGRQMLMF
                     LAPKA"
     gene            1195..1392
                     /gene="rpmI"
                     /locus_tag="ACZPE2_01980"
     CDS             1195..1392
                     /gene="rpmI"
                     /locus_tag="ACZPE2_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1318647.1"
                     /translation="MPKIKTHSGAKKRFKLSKNGKPIRAHANKSHILTKKNTKRKRNL
                     RKTSVADTTNVKQIKRLIPYK"
     gene            1444..>1518
                     /locus_tag="ACZPE2_01985"
     CDS             1444..>1518
                     /locus_tag="ACZPE2_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1318648.1"
                     /translation="MARVKGAMMTRKRRNKTLKLAKGYY"
     assembly_gap    1519..1528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1529..1779
                     /gene="rplT"
                     /locus_tag="ACZPE2_01990"
     CDS             <1529..1779
                     /gene="rplT"
                     /locus_tag="ACZPE2_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1318649.1"
                     /translation="EAVMKSGQYAYIGRKQKKRDFRQLWITRISAACKMNGMNYSTFI
                     NGLKKAGIDLNRKMLSEIAISDPAAFTTLTEKAKEALK"
     gene            1940..2716
                     /locus_tag="ACZPE2_01995"
     CDS             1940..2716
                     /locus_tag="ACZPE2_01995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1318650.1"
                     /translation="MRITSRNNEKIKAFVKLRDSARARNTEGLFVLEGARLCFDALES
                     GYNVQELFYTETALSRYAERVTALIENSVAAFEAADEVAQKLADTVNSQGVFCTVKMK
                     ESAEICPSSPKKYVALDGIQNPDNLGAISRTAEALGVDALIVGGGCDIYNPKALRASM
                     GALLRLPVISVPSILPVLQKARENGLPVLSTVPDSAAEDITTVDFSHGAVTVIGNEGN
                     GVSKEVKAFSDRFITIPMGGKAESLNASAAATITMWEMMK"
     gene            2698..>2992
                     /locus_tag="ACZPE2_02000"
     CDS             2698..>2992
                     /locus_tag="ACZPE2_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318651.1"
                     /translation="MGNDEMNNESVYWLWLAKTLGAGAKTADIINYFGSARMLYEKGS
                     REWRLSGILTASQINKLSLFSPSQFAETLRVCVDNGWRIITPDSEYYPKRLREI"
BASE COUNT          893 a          653 c          728 g          708 t
ORIGIN      
        1 tgtgccgtcc tcgccgtttt tgccgtgcat tacgggaaag cagacgtcaa tatgtatctt
       61 ttcgggaccg gactccctga aaaccgtgat gccgtggtct tttctgtcgg gcgagagcaa
      121 agcctttgtg catttgccgc tttttatcca tttatcctcc ggcaaaagag aaacgtcgcc
      181 ctcaaagaga aaccattcgc cctccttggt tataccgagc gggcaaacct cgtatttatc
      241 gcgcggaatg ttgcttacaa cgtatgaagc cgatacgcac gaaacgtcgt gctcgcttga
      301 tactccgccg aaaagaacga gaaccttctt cataatttat caccccgtga aaatttcaga
      361 aaataaattg agctgagata taattataca tatactaata aatgataaca tattacggcg
      421 gcataatcaa tctttgttta aatttaattt caaaggccgc aaaaagtcct tgacaaagcg
      481 caaaagaagt catataatac cgttacaaca aagtagagca gttcgttctc acccgtgatt
      541 ttaagatgaa acgggtcaat acatcggggt tatcccttat taagtgtgtt gagtgcgagc
      601 ggttatctgt tcgcactttt gttattttat tttaagaacg gagtgattac ctatcggtac
      661 caaagaaatg cagatcaatg aagacatccg tgacagggaa gtacgcgtag taaccgacgg
      721 cggagagcag ctcggcataa tgtcggctaa ggaggctctt aaaaaggcag aggagagaaa
      781 cctcgacctt gttaaaatat cccccaacgc aaagccgccc gtttgtaaaa tcatggatta
      841 cggcaaatac cgttttgagc agtcaaagcg cgaaaaggaa aacagaaaaa accagcgcgt
      901 tattgaaatc aaggagattc gtctttcgct caacattgat actcacgact ttgagacgaa
      961 ggtcaatcac gctcacaaat tcttaaaggc cggaaataag gtcaaggttt ccatccgttt
     1021 cagaggccgt gaaatggctc atccgcagat aggtgaggtt tctatgaaac gctttgccga
     1081 agcttgcgaa gagtttgcaa cagtcgaaaa accggcgaag ctcgaggggc gtcagatgct
     1141 gatgttcctt gcgcccaagg cgtgaccgta aaaaatatca ggaggaattt tgcaatgcct
     1201 aagatcaaaa cacacagcgg agctaaaaaa cgcttcaaat tatcaaagaa cggcaagccg
     1261 ataagagcac atgccaacaa gtctcatatc cttaccaaaa agaacacaaa gagaaagaga
     1321 aatcttcgca agacatccgt tgccgataca actaatgtta agcagattaa acgtctcatt
     1381 ccttataaat aatcggagcg gtttatatac cgccatactt aaacattcgg aggtaactaa
     1441 ctaatggcac gtgttaaagg tgcgatgatg actcgcaaaa gaagaaataa aacattaaag
     1501 cttgctaagg gctactacnn nnnnnnnnag gaagcggtta tgaagtccgg ccagtatgct
     1561 tatatcggca gaaagcagaa gaagagagat ttcagacagc tttggataac aagaatctcg
     1621 gctgcctgca agatgaacgg tatgaattat tcaaccttca taaacggcct taaaaaagcc
     1681 ggtatcgact taaaccgcaa gatgctttcg gagattgcga tttcggatcc tgcggcgttt
     1741 accacgctca ccgaaaaagc taaagaagcg cttaaataag ttattgccac gcctgagagt
     1801 ttccctcagg cgtgtttttc cttttaatta cggcacttcg gttttgcttt aaatcacgtg
     1861 cattcgggac ttttttgcac aaggcttcaa aggcgcgcga tctaaggaaa aaccgatgct
     1921 cgcatcacag gagaatttta tgcggataac gagcagaaat aacgaaaaaa tcaaagcctt
     1981 cgtaaagctg cgcgacagtg cgagagcgcg aaatacagag gggctttttg tccttgaggg
     2041 agcaaggctt tgctttgacg ctcttgagag cggatacaat gtgcaggagc ttttttatac
     2101 cgagacggca ctttcgcgtt acgctgaaag ggtgactgcg cttatagaaa acagcgttgc
     2161 ggcttttgaa gctgcggacg aggtggcgca aaagcttgcg gataccgtga attcacaggg
     2221 cgtgttttgt acggtaaaaa tgaaagaatc ggcggagata tgcccgtctt caccgaaaaa
     2281 atacgttgca cttgacggta ttcaaaatcc cgacaatctc ggcgcgatat cacgtaccgc
     2341 agaggcactc ggagttgatg cgctgatagt cggcggtggc tgcgatatat ataatcccaa
     2401 agccctcaga gcgtcaatgg gcgcactttt gagacttccc gtcataagcg tgccgtctat
     2461 tttaccggtg ctgcaaaagg cgagggaaaa cggcttaccc gtgttgtcaa cggtgccgga
     2521 ttccgcggca gaggatataa cgactgtcga tttttcgcac ggagcggtca ccgttatagg
     2581 gaacgaggga aacggcgtgt caaaggaagt aaaggctttt tccgacaggt tcataacaat
     2641 accgatgggc ggcaaggcgg agtcgcttaa tgcttccgct gccgctacga taacaatgtg
     2701 ggaaatgatg aaatgaacaa tgagagtgtt tactggctct ggctcgcaaa aactctcgga
     2761 gcgggggcca aaactgcgga tattataaat tacttcggaa gcgcgcgaat gctctatgaa
     2821 aagggcagtc gcgaatggcg gctttcgggt atacttacgg cgtcacagat aaacaagctg
     2881 tcgcttttca gcccgtcgca attcgccgag acgcttcgtg tttgcgtcga taacggctgg
     2941 cgcataataa cgcccgacag cgaatattat ccgaaaagac tcagagaaat cg
//
LOCUS       DBMDVO010000084         2982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15267_length_2982_cov_1.338424, whole genome shotgun sequence.
ACCESSION   DBMDVO010000084 DBMDVO010000000
VERSION     DBMDVO010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15267_length_2982_cov_1.338424"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(42..1490)
                     /locus_tag="ACZPE2_02005"
     CDS             complement(42..1490)
                     /locus_tag="ACZPE2_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1318652.1"
                     /translation="MRKALSMVFAIVLTMPFLSLLSGAMTEKTLYADMPVEWAVSYDP
                     RTDASDLVPESDNKALPFFSDGADTPYTFYTFLNSNQRAVYNTVKEKLFESSIDVALP
                     SPLTWDGTSTSVPSNIKSALSDAVTGGLSALCDDYPMIFWINGYSISYGYSYYQTSSG
                     YHFTISSVTVKPRINTNAYADMNKVKQDYNDMAAVVDSVEIKGATRYEKVKFIHDFIC
                     KRVEYDEKFEIPTAHEPTSVFLTPYKTVCEGYSESFKILCDKAGIPCVIAVGNSNGVG
                     HAWNYVKMEDGKWYGVDCTFDDQGNVLYDYFLVGTASGNRYFGASETFGSSHTETGKR
                     YGGSFTLTYPTVSENAYSPIVPEINSGATVNEKSKLLYITNGASVNSAVYMQSGYSFA
                     SDGNKTGSIFTVSNTSLGTSTGYTVIMRGDVVPSGYVDGSDFDAVVNHSVEDKKLGDG
                     SSEYLAADVNGDGVVDLFDAAEIDLIKAGKAS"
     gene            complement(1614..2069)
                     /gene="nrdR"
                     /locus_tag="ACZPE2_02010"
     CDS             complement(1614..2069)
                     /gene="nrdR"
                     /locus_tag="ACZPE2_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1318653.1"
                     /translation="MKCPYCGYEESKVIDSRPTDEGERIRRRRECIKCGKRFTTYEVI
                     ESVPILVVKKDKSREVFDRNKLLGGMMRACEKRQVSMDTLERAVDDIEANLQNSLDRE
                     VSSMHIGELAMQKLKDIDEVAYVRFASVYRQFKDINAFMDELSQLLRKR"
     gene            complement(2164..>2982)
                     /locus_tag="ACZPE2_02015"
     CDS             complement(2164..>2982)
                     /locus_tag="ACZPE2_02015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HIR1318654.1"
                     /translation="RLHIPIFHMEAGNRCKDECLPEETNRRIVDIISDVNMAYSEHAR
                     RYLAECGLPKERTYVTGSPMAEVLHKNLEKIENSDILTKLGLEKKKYILLSAHREENI
                     DTEKNFNSLFSAINGLAEKYDMPILYSCHPRSKKRLEESGFKLDERVIRHEPLGFHDY
                     NNLQMNAFAVVSDSGTLPEESSFFTSVGKNFPAVCIRTSTERPEALDKGCFVLAGIDE
                     KSLLQAVDTAVELDKNGFCGIPVPDYTDENVSTKVVKIIQSYTGVVDKMVWRKL"
BASE COUNT          738 a          751 c          630 g          863 t
ORIGIN      
        1 tgcgattttt gcggtgtcgc ttttattatc aaattacagc tttatgaagc tttgcctgct
       61 tttatcaagt ctatctccgc ggcgtcaaat aaatctacaa ctccgtcgcc gtttacgtcg
      121 gcagccaaat attcgcttga accgtctccg agctttttat cctcaacgga atgattaacc
      181 acggcgtcaa agtcgctgcc gtccacgtat ccgctcggca caacgtcgcc gcgcataata
      241 acggtatatc ccgtgcttgt gcccaaagac gtattgctga cagtaaatat cgagcctgtt
      301 ttattgccgt cggaagcgaa actgtaaccg ctctgcatat aaaccgccga atttacgctc
      361 gcaccgtttg tgatatacaa aagctttgac ttttcgttca cagttgcgcc ggagtttatt
      421 tccggtacaa taggcgaata ggcattttcc gatacggtgg gatatgtcag cgtaaagctg
      481 ccgccgtaac gttttcccgt ttcggtatgg ctgctgccga aggtttcact cgcgccgaaa
      541 tacctgtttc cgctggctgt tcccacaagg aaatagtcat acaaaacatt gccctgatcg
      601 tcaaaggtgc agtcaacgcc gtaccacttg ccgtcctcca tttttacgta attccaagcg
      661 tgacctacgc cgttcgaatt gcccacagct attacgcacg gtattccggc tttgtcgcag
      721 aggattttaa acgactcgct gtaaccctca cacacggttt tgtacggtgt caaaaatacg
      781 ctcgtcggct catgtgcggt aggtatttca aacttttcat catactcgac tcgtttgcag
      841 ataaaatcgt gtatgaactt caccttttcg tagcgcgttg cgcctttgat ttctacgctg
      901 tcaacaacag ccgccatatc gttataatcc tgctttacct tgttcatatc ggcataggcg
      961 tttgtgttta ttcgcggctt caccgtaacc gatgaaatcg taaagtgata accgctactt
     1021 gtttgataat aggaataccc gtagcttatg ctgtacccgt ttatccaaaa aatcatcggg
     1081 taatcgtcgc aaagtgccga taatccgccc gtgaccgcat ctgacagggc tgattttatg
     1141 ttgctcggca cacttgtgct tgtgccgtcc caggtaagcg gcgacggcaa ggctacatca
     1201 atcgagcttt caaacagctt ttctttgacc gtgttataaa ccgctcgttg gtttgaattc
     1261 aaaaatgtat aaaaggtata cggagtatcc gctccgtccg aaaagaacgg caatgcttta
     1321 ttgtcgcttt ccggaaccaa atcggaagcg tccgttctcg ggtcatagct gacagcccac
     1381 tctacgggca tatccgcgta aagcgttttt tctgtcattg cccccgataa gagtgacaaa
     1441 aacggcatgg tcaagactat tgcaaaaacc atactcaaag cttttctcaa gacaaatccc
     1501 tcttttctgc aaatttttat catctgatta tataatatat tatttcggct gagattgtca
     1561 acaaaacaac aaagcagagg ccttatatac aaggtctctg ccgaatacaa atcttatctt
     1621 tttctgagaa gttgcgacag ctcgtccata aaggcgttta tatctttaaa ctgcctgtaa
     1681 accgaagcga acctgacata ggcaacctcg tctatgtcct taagcttctg catggcaagc
     1741 tcgcctatgt gcattgacga aacctcacgg tcaagagaat tttgaagatt tgcctcaata
     1801 tcgtcaacgg cgcgctccaa ggtatccatc gagacctgac gcttttcgca ggcgcgcatc
     1861 ataccgccca aaagcttatt gcggtcaaac acctcgcgag acttatcttt ttttacgacg
     1921 agtatcggca cgctttcaat gacctcatat gtagtaaagc gcttaccgca cttgatgcac
     1981 tcgcggcggc ggcgaattct ctcgccctcg tcggtaggtc ttgagtctat taccttgctc
     2041 tcctcgtatc cgcagtaagg gcacttcacg gcaaatcaca tccaatagtt atttttaatc
     2101 taagattatt ataacaaaca aaatttacat tgtaaagcgt attttagccg acgctttgat
     2161 atgtcaaagc tttctccaaa ccattttatc gactacacct gtatacgact gaattatttt
     2221 gacaacctta gtcgaaacgt tttcatcggt ataatctggc acggggattc cgcaaaagcc
     2281 gtttttgtca agctcaaccg cagtatcaac cgcttgcagc aatgattttt catcaattcc
     2341 ggcaagcacg aagcatccct tatcgagagc ctcgggacgt tctgtggagg ttctgatgca
     2401 aaccgcaggg aagttctttc cgactgaagt aaagaagctg ctctcctcgg gcagcgttcc
     2461 gctgtcggaa accacggcaa acgcgttcat ctgaagatta ttgtaatcgt gaaatccgag
     2521 cggctcgtgg cgaataactc tctcgtcaag cttaaagccg ctctcctcga gacgtttttt
     2581 tgaacgcgga tgacacgaat agagtatcgg catatcatat ttttccgcaa ggccgttaat
     2641 tgcggagaac aggctgttga aatttttttc ggtgtcgatg ttctcttctc tatgagccga
     2701 cagaagaata tactttttct tttcgagtcc gagctttgtt aaaatgtcgc tgttctctat
     2761 tttttcaagg tttttatgaa gcacctccgc catgggggag ccggtgacgt atgtgcgctc
     2821 cttcggaagc ccgcattcgg cgagatatct tcttgcgtgt tcggaataag ccatattcac
     2881 gtccgaaata atatccacta tacggcggtt tgtctcttcc ggcaaacact cgtccttgca
     2941 gcggttgccc gcctccatat ggaatatcgg tatatgaagt ct
//
LOCUS       DBMDVO010000085         2982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15270_length_2982_cov_1.259285, whole genome shotgun sequence.
ACCESSION   DBMDVO010000085 DBMDVO010000000
VERSION     DBMDVO010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15270_length_2982_cov_1.259285"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..1053
                     /gene="ispG"
                     /locus_tag="ACZPE2_02020"
                     /gene_synonym="gcpE"
     CDS             1..1053
                     /gene="ispG"
                     /locus_tag="ACZPE2_02020"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1318655.1"
                     /translation="MPERRKSRVVTAGNVKIGGNNHIPVQSMLNIPAEDAEGNVKQAL
                     ELQKAGCEIIRLTVPNKEAVKTLAAVKEKVSIPVVADIHFDYRCALESVAAGADKIRI
                     NPGNIGSDDRVKAVADACRAKNIPIRIGVNSGSLEKDILAKYGAPTPDALCESALYHA
                     GLLEKFDFHDIVISIKSSDVPTMVSAYRKIATMCDYPLHLGVTEAGTRRMGLIKSAAG
                     IGSLLMDGIGDTIRVSLTADPVEEVSAGFDILKAVDIKKDCPQIVSCPTCGRTKIDLI
                     SLAEKIENALRGCRKPIKVAVMGCVVNGPGEAKEADIGVAGGDGCGMIFKHGEILKKV
                     SEDEIVPELLKEIEKM"
     gene            1072..>2982
                     /locus_tag="ACZPE2_02025"
     CDS             1072..>2982
                     /locus_tag="ACZPE2_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1318656.1"
                     /translation="MTSFYDLFIEYRNDFADMDAVLGDAQIIDMSAESAERRLYIKVR
                     FPRLVSEKTLDKISEIIRDRLGLGAVKIAPVFSSSLFSDRYSGEISEWAKKNVPMANG
                     FFVDCKYDFSEDEIKIELMHGGKQILEDVGAQNLISKMLRERFGVSKELSFVQRDDYD
                     ARDDISAAQKKIDSMAPKAAPVKSGSSRSFDPVKEDDTPKEHIVKNGIPYYLESVKPI
                     FGSNIRSQPIKIDEITLPEEGDTTPVTIYGEVFGIEVKDTKKGKVKIVSFNITDNTNS
                     FSAVMLPKVEHCDELLSKLKNGAHILMFGEVEYDTYRGDYTIKPKCISTVQMIEKEDN
                     YPEKRVELHLHTNMSQMDGMTPPSKLVERAIKWGHKAIAITDHGCVQGYPEACNAAAG
                     KIKIIYGIEDYFIDDIKEPDKTYKELRSYHQIILVKNHIGLKNLYKLISKSNIDYFYK
                     KPRMPKSEIMKHREGLIIGSACEAGELYRAILEERPEQEIMEIASFYDYLEIQPVGNN
                     RFMIDAHSDPSAKNPDKNKEFDKITCIEDIENINRKVIAIADKLGKPVVATCDVHFID
                     PEDAKYRAILMAAQGFTDADKQAPLYFRTTEEMLNEFSYLGEETAHEIVIENPNKIAD
                     MIDVVRPFPIGTF"
BASE COUNT          897 a          679 c          750 g          656 t
ORIGIN      
        1 atgccagaaa gacgaaaaag ccgcgttgta acggcaggta acgtaaaaat aggcggaaac
       61 aaccacatac ccgttcagtc gatgttaaac attccggccg aggacgcaga gggcaatgtt
      121 aagcaggcat tggagctgca aaaagccggt tgtgagatta tccgtctcac ggttcccaat
      181 aaagaggcgg taaagacctt agccgccgta aaagaaaagg tttcgattcc ggttgttgcg
      241 gacatacatt ttgactaccg ctgcgcgctt gagagcgttg cggcaggtgc cgataaaatc
      301 cgcataaatc ccggcaacat cggctccgac gatcgcgtta aggcggttgc cgacgcatgc
      361 cgtgcaaaaa acattccgat aaggataggc gtaaattcag gctcgcttga aaaggatatt
      421 ctcgcaaaat acggggctcc cacgcctgat gcactttgcg agagcgcgct ttatcacgca
      481 gggcttctcg aaaaattcga ttttcacgat atagtcatct ccataaaatc ctccgacgtg
      541 ccgaccatgg tatctgcata cagaaaaata gcgactatgt gcgattatcc gcttcatctc
      601 ggcgtaaccg aggcaggtac gcgccgtatg gggctcatta aatccgccgc cggaataggc
      661 tcgcttttaa tggacggcat aggcgacaca atacgtgttt ctcttaccgc cgaccccgta
      721 gaggaggtct cggcaggctt tgatatatta aaggcggttg acataaaaaa ggactgtccg
      781 cagatagtgt cgtgtccgac ctgcggcagg accaaaatcg atttgatttc tcttgccgaa
      841 aaaatcgaaa acgctttaag aggctgccgt aagcccatta aggttgccgt tatggggtgc
      901 gtcgtaaacg gtccgggcga agctaaggag gcagacatcg gcgtagccgg cggcgacggc
      961 tgcggtatga tattcaagca cggcgaaata ctcaaaaaag taagcgagga tgaaatagtt
     1021 ccggagctgc ttaaagaaat agaaaaaatg taattgtgaa agagacggca aatgacaagc
     1081 ttttatgacc tgtttataga atacagaaac gatttcgcgg atatggacgc ggtactcggc
     1141 gacgctcaaa taattgatat gagcgcagag tctgccgaaa gaaggctgta cattaaggtg
     1201 cgctttccgc gtcttgtgtc cgagaaaaca cttgataaaa taagcgaaat aataagagac
     1261 aggctcggac tcggcgcggt taaaatagcg ccggtctttt cttcgtcgct gttctcggat
     1321 aggtattcgg gcgagataag cgagtgggca aagaaaaacg tgcctatggc aaacggattt
     1381 tttgttgact gcaaatatga tttttccgaa gatgaaatca aaatagagct catgcacggc
     1441 ggtaagcaaa ttcttgagga cgtcggcgcg caaaatctca tttctaaaat gctccgtgaa
     1501 cgcttcggag tatcaaaaga gctttctttt gttcagaggg acgactatga tgcacgcgat
     1561 gacatttccg cggcgcagaa aaaaatcgac agtatggctc ccaaggccgc acccgtaaag
     1621 agcggttcat cgcgcagctt tgaccccgtc aaggaggacg atacgccaaa ggagcacatt
     1681 gtaaagaacg gcataccgta ttatcttgag agtgtaaagc cgattttcgg cagtaatatc
     1741 agaagtcagc ccataaaaat agacgaaatc actttgcccg aagagggtga cacgacaccc
     1801 gttacgattt acggcgaggt tttcggcatt gaggtcaagg atacgaaaaa aggcaaggta
     1861 aagatagtat cctttaacat aaccgacaat acaaattcgt tttccgccgt tatgttgcca
     1921 aaggttgaac attgcgacga gctgctgtca aagctgaaaa acggggcgca catacttatg
     1981 ttcggcgagg tcgaatatga cacgtaccgc ggcgattaca caataaaacc taagtgcata
     2041 agcaccgttc agatgataga aaaagaggac aattaccccg aaaagcgcgt tgagcttcat
     2101 cttcacacaa atatgtcgca gatggacggt atgactcctc cctcaaagct tgtggagcgc
     2161 gctataaagt gggggcataa ggcaatagcc ataaccgacc acggctgtgt tcagggttac
     2221 cccgaggcgt gcaacgccgc cgccggcaaa atcaaaatca tttacggcat tgaggattat
     2281 tttatagatg acatcaagga gccggataaa acatataagg agcttcgctc ctatcaccag
     2341 ataattctcg taaagaatca tatagggctt aaaaatctgt ataagctcat ttcaaagtcc
     2401 aatatagact atttttacaa aaagccgaga atgccaaaga gcgaaataat gaaacaccgc
     2461 gagggcctta taatcggctc ggcgtgcgag gcgggcgagc tttaccgcgc tatactcgag
     2521 gaacgccccg aacaggagat tatggagata gcgtcgtttt acgactatct cgaaattcag
     2581 cccgtaggca acaaccgttt tatgattgac gcccattccg acccatcggc gaaaaatccg
     2641 gacaaaaaca aggaatttga caagataacc tgtattgagg atatagaaaa tataaaccgc
     2701 aaggttatag ctattgccga caagctcggt aagcccgtgg ttgccacctg cgacgtccac
     2761 tttatagacc ccgaggacgc aaaataccgc gctattctta tggcagctca gggctttacc
     2821 gacgctgata agcaggctcc gctgtacttc agaacgaccg aggaaatgct caatgagttt
     2881 tcatatctcg gcgaggaaac cgcgcacgaa atagtcatag aaaatcccaa caagatagcc
     2941 gatatgatag atgttgtacg cccgttcccg ataggcacgt tc
//
LOCUS       DBMDVO010000086         2966 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15387_length_2966_cov_1.272949, whole genome shotgun sequence.
ACCESSION   DBMDVO010000086 DBMDVO010000000
VERSION     DBMDVO010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15387_length_2966_cov_1.272949"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="ACZPE2_02030"
     CDS             complement(<1..391)
                     /locus_tag="ACZPE2_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR1318657.1"
                     /translation="METLEGLKRTDYCGTLTAKDVGRTVTLCGWVQRQRDLGSLIFID
                     LRDRTGIVQLAFDENTDKAVFDKAFSARSEYVLAAVGTVRERSAKNTEIATGEIEVEV
                     TELRILGKSETPPFEIVPNCQTAETTRL"
     gene            complement(391..1725)
                     /gene="hisS"
                     /locus_tag="ACZPE2_02035"
     CDS             complement(391..1725)
                     /gene="hisS"
                     /locus_tag="ACZPE2_02035"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1318658.1"
                     /translation="MSLITSSIKGTLDLLPKDSYKTQYIEAAMREIAENYGFYEMRTP
                     VFEHTELFERGVGDTTDVVQKEMYTFSDKGGRSITLRPEGTSGAVRAFLENGLFNEPM
                     PQKIYYLTSCYRYEKPQAGRLREFHQFGVECFGAGAPSQDAEIIALANEVFNYLGVSN
                     LSLEINSIGCKKCRAEYHKALKEYFASHIDELCDTCRGRLEKNPMRILDCKSPICSEI
                     AKGAPVITDYLCDDCREHFDSVKKYLDAMNIAYTVNPRIVRGLDYYTRTVFEFVSNEI
                     GAQGTVCGGGRYDGLIEEVGGNPLPACGFGMGIERLLLLMEAQKTPFPERKKCDIFIV
                     SMGDEANIKAAELATDLRNEGLSAQFDTVGRGFKAQMKYANKIGALYTVVLGENELSS
                     GNVKLKNMADGTEEETTLKDFTDNFQSIVIRQAMAGFDTEGIDIKDILGGNA"
     gene            complement(1822..2223)
                     /locus_tag="ACZPE2_02040"
     CDS             complement(1822..2223)
                     /locus_tag="ACZPE2_02040"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1318659.1"
                     /translation="MNIALMAHDEKKELMVQFCIAYCGILSKHNLCATGTTGKLVSEA
                     TGLKIQQFLSGSQGGDQQIGARIACNEIDLLLMFRDPLNPKPHEPKENNLLRLCDVHN
                     IPVATNIATAEALIHSLERGDLDWRDIVNPK"
     gene            complement(2383..>2966)
                     /locus_tag="ACZPE2_02045"
     CDS             complement(2383..>2966)
                     /locus_tag="ACZPE2_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1318660.1"
                     /translation="DLLLETGEDILFNWYDAARGDCKKKDAVYDTLYKGVSLLPAPKS
                     TEELTYDEFYELILSFESSYDYILFDAENCGTAFDFAAKICDKAIIVTTPDAVSLRSA
                     FDKAEKIRPLSDEARLIVNKVIRSEMEEGRQTKLNDCVDISSVKLLGVVPYDSSAMHL
                     SDGGRLSGFIGSAFSRIAERLLGKNVPFSPKYF"
BASE COUNT          689 a          746 c          706 g          825 t
ORIGIN      
        1 taagccttgt agtctcggcg gtctggcagt tcggaactat ctcaaacgga ggagtttcac
       61 tcttgccgag aatccgaagc tcagttacct caacctcgat ttcgcctgtg gcaatctcgg
      121 tgttttttgc ggaacgctct cttaccgtgc cgacggctgc aagaacatac tcacttcttg
      181 cggaaaaggc cttatcaaac acggccttgt cggtgttttc gtcaaatgcg agctgaacta
      241 tgcccgttct gtcgcgcaaa tctataaata tgagtgagcc gaggtcacgc tgacgctgaa
      301 cccagccgca gagcgtgacg gttcttccga cgtcctttgc ggtgagtgtg ccgcagtagt
      361 cggtgcgttt aagtccctca agagtttcca ttatgcgttt ccccctaaaa tatctttaat
      421 atcaatgcct tcggtgtcga agcccgccat tgcctggcga attactatcg attggaaatt
      481 atcggtgaag tccttgagcg tggtctcctc ctccgtgccg tcagccatat ttttaagctt
      541 gacatttccc gaggacagct cattttcgcc gagaaccacg gtgtagagag caccgatttt
      601 gtttgcgtat ttcatctgcg ccttaaagcc cctgcctact gtgtcaaact gagcggaaag
      661 cccctcgttg cgaaggtcgg ttgcaagctc ggcagccttg atatttgctt cgtcgcccat
      721 ggatactata aatatatcgc actttttgcg ctcggggaac ggagtttttt gcgcctccat
      781 caaaaggagc agcctctcta tgcccatacc gaagccgcac gccggaagcg gatttccgcc
      841 gacctcctct attaagccgt catatcttcc tccgccgcat acagtgccct gcgcgcctat
      901 ttcgtttgag acaaactcaa acacggtgcg tgtgtagtag tcaagtccgc ggacgatgcg
      961 cggatttacc gtataggcta tattcatggc gtcgagatat ttttttaccg agtcaaagtg
     1021 ctcgcggcaa tcgtcgcaga gataatctgt tatgacgggc gcgccctttg cgatttcgga
     1081 gcatatggga cttttgcagt caagtattct catcggattt ttttcgagtc tgccgcggca
     1141 cgtgtcgcaa agctcgtcaa tatgggatgc aaaatattcc ttcaatgcct tgtgatactc
     1201 cgcgcggcat tttttgcagc cgatggagtt tatttcgagt gaaagatttg atacgccgag
     1261 gtagttgaac acctcgtttg cgagtgctat aatttccgca tcctgcgagg gagcacctgc
     1321 gccgaagcat tccacgccga actggtggaa ttcgcggagc ctgcctgcct gcggtttttc
     1381 gtagcggtag catgaggtga ggtagtagat tttttgcggc atcggctcgt tgaaaagccc
     1441 gttttcgagg aatgctctta ccgcgcctga cgtgccctcg ggacggagcg ttatcgaacg
     1501 tccgccttta tcggaaaagg tgtacatctc tttttgaact acgtctgtgg tgtcgcccac
     1561 gccgcgctca aaaagctcgg tatgctcaaa aaccggcgtg cgcatttcgt aaaagccgta
     1621 gttttcggct atctcgcgca ttgccgcttc gatatactgg gttttgtagc tgtccttggg
     1681 cagcaaatcg agagtaccct ttatggagct tgttataagt gacatctcaa atatcctttc
     1741 ggttggggat tatatcagtg acgcacaggg tgcgtaacac tataatgccc cctctgttcg
     1801 gagggggcga aaaaatactg cttatttcgg atttacaata tcacgccagt caagatcgcc
     1861 tctttcaagg ctgtgtataa gcgcctctgc cgttgcaatg tttgttgcaa ccggaatatt
     1921 gtgtacgtcg caaagcctta aaaggttatt ctccttaggc tcatgcggct tcggattgag
     1981 cggatctcgg aacatcaaga gaaggtctat ctcgttgcag gctatacgcg cgcctatctg
     2041 ctggtcgccg ccctgcgagc cgcttaaaaa ctgctgtatt ttaagaccgg tagcttcgga
     2101 aacaagtttt cccgtagtgc cggttgcgca aagattatgc ttagacaaaa ttccgcaata
     2161 tgctatgcaa aactgaacca taagttcctt tttttcatcg tgagccatca aagctatatt
     2221 cataaaagtt cctccgatct tttattcgtt tgtgttttat acaacttaaa atattctccg
     2281 atagatataa taacaaaaaa aagtcaactt ttcaagattt ttttcatttt gcacaaaaat
     2341 agtgtggatt acatccggta cagcacccca aaatacccta aattaaaaat atttcggcga
     2401 aaacggcaca tttttgccga gaagacgctc ggcgattcga gaaaaagcac tccctataaa
     2461 gccggaaagc ctgccgccat cgctcaaatg catcgcggac gaatcatacg ggacaactcc
     2521 gagcagcttt acggaggaaa tatccacgca gtcgttgagc tttgtctgcc tgccctcctc
     2581 catttcggag cgtatcacct tattcactat aagccgcgcc tcgtcggaaa gaggtcttat
     2641 tttttcggcc ttgtcaaagg cgcttcgcag agatacggcg tcgggcgtcg tcacgattat
     2701 cgccttgtcg cagatttttg ctgcgaaatc aaaggctgtg ccgcaattct cggcgtcaaa
     2761 caatatataa tcatagctgc tctcaaaaga gagtataagc tcataaaatt cgtcgtatgt
     2821 aagctcttcc gtgctttttg gggccggcaa aagcgaaacg cccttatata atgtatcgta
     2881 aacggcgtct ttttttttgc agtcgcccct ggcggcgtca taccaattaa aaagtatgtc
     2941 ctcgcccgtt tcgaggagca ggtcga
//
LOCUS       DBMDVO010000087         2961 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15435_length_2961_cov_1.277273, whole genome shotgun sequence.
ACCESSION   DBMDVO010000087 DBMDVO010000000
VERSION     DBMDVO010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15435_length_2961_cov_1.277273"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="leuB"
                     /locus_tag="ACZPE2_02050"
     CDS             <1..755
                     /gene="leuB"
                     /locus_tag="ACZPE2_02050"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1318661.1"
                     /translation="RPARIFAPLKDASPIKDSITEGNLDIMIVRELTGGIYFGERGTG
                     VENGEEYAYDTEKYTVSEIKRIAKTAFEMAMKRSKKLTSVDKANVLDSSRLWRKTVIE
                     MSKDYPEVELNHMYVDNCAMQLVRNPKQFDVIVTSNIFGDILSDEASMISGSIGMLAS
                     ASLSDGKFGLYEPIHGSAPDIAGFGVANPLATILSAAMMLKYSLDESDAAADIENAVN
                     AALENVRTPDIYKDGFRKVSTSGMGDEVIKRL"
     gene            931..2394
                     /locus_tag="ACZPE2_02055"
     CDS             931..2394
                     /locus_tag="ACZPE2_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1318662.1"
                     /translation="MDENKDKTLSGGNDESYGSTFDRNTEDVKKEMEELAATFQRELD
                     KTKAEAEEKGELIQELEDIPEASTRNDDEEPSEAELCECCGEKRRGTKADPDSPYCEE
                     CDLGLRHYPFSFLNIFFVIVVIALVFYSGYVFAGRMGVLNDVYKADKYVSQKKMDSAV
                     TAYEDTVSAMEKAGIKGELVYKRQIMANYRIGYLQDIDSMSGNINSWELSLPHFRSVK
                     KAMGESADFIATVRKAYTILTKYDVNDPSDFPYDDAMAALVSLESEPYTEDTTANTSS
                     ATTSSVKATQYNKAMVYYYEYYISVICNKDNDEQLKYLEKIKEEYPQYIWLYGSALGD
                     LYAKTGKDVTEICDKIRSNNSEDPSPDLIEIESLRIKGDYDAAIAKCEEYASPDDCTV
                     KYEIYRQEALCYILKDDYDTALETAKKAYDDNGNSLEVIDTLALCAVLKNDDATYSSM
                     ESLLDGSGYSLSDKVTGFKAGTVTLDDILLKGAYDVE"
     gene            2391..>2961
                     /locus_tag="ACZPE2_02060"
     CDS             2391..>2961
                     /locus_tag="ACZPE2_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318663.1"
                     /translation="MRAVYLTVKYITIVGTFLQGFFEHLSCRMYDVLIEDGRYLRSNE
                     MCGHIEHELIKKRGTAFGVCFFPFLFNLLIGLILTAAGSVNIFYLGEFFMSADSKVPN
                     FPNFLFLWVGISCLTNLFPQIEDALTLKEQIYGKGKSNLFVKIIAAPIFAVLYVGSYL
                     QSVGLTLVTSVVFSLFMPSILGTFIPQLYN"
BASE COUNT          831 a          630 c          725 g          775 t
ORIGIN      
        1 tccgtcctgc aagaatattt gcccccttaa aagacgcgtc gccgattaaa gacagcataa
       61 ccgagggtaa ccttgacata atgatagttc gcgagcttac cggcggaatt tacttcgggg
      121 agcgcggcac gggtgtcgaa aacggcgagg aatacgctta tgacaccgaa aagtacaccg
      181 tttccgaaat taagagaatt gcgaaaaccg cttttgaaat ggcaatgaag agaagcaaaa
      241 agctcacaag cgttgacaag gcaaatgtgc tcgattcatc aagactgtgg agaaagacgg
      301 ttatcgagat gtcaaaggac tatcccgagg tagagcttaa ccatatgtat gttgataatt
      361 gcgccatgca gcttgtacgc aatccaaagc agtttgatgt tatagttacg tcaaacatat
      421 tcggcgacat tctctcggac gaagcgtcta tgatatcggg ctcgatagga atgctcgctt
      481 ccgcttcgct ctcggacgga aaattcggac tctatgagcc gatacacggc tctgcgccgg
      541 atattgcagg cttcggcgtc gcaaatccgc ttgccaccat actctccgca gccatgatgc
      601 tcaaatactc tcttgatgag agtgacgctg cggcggatat agaaaacgcg gtaaacgcag
      661 cactcgaaaa tgttcgcact cccgatatat acaaagacgg atttagaaag gtatctactt
      721 cgggtatggg cgacgaggtt attaagcgcc tttaataaat agcagaatat tgcttcggcg
      781 gctccgtttt tacggagtcg ctttttaatt tgtgaacaaa aactgaattt atcgtatttt
      841 gtttgaaata tcataaagta agtgttaata tatagaggta ttgtacttgt ttggtacaac
      901 gccgtaataa tttgcaagga ggattttccc ttggacgaaa ataaagacaa gactttatcc
      961 ggcggaaacg acgagagcta cggctcaaca tttgaccgca ataccgagga tgtcaaaaag
     1021 gaaatggaag agcttgcggc tacatttcag cgcgagcttg acaaaaccaa ggcggaggca
     1081 gaggaaaaag gcgagcttat acaggagctt gaggatatcc ccgaggcttc gaccagaaat
     1141 gatgacgagg agccttcgga agccgagctt tgcgaatgct gcggtgagaa aaggcgcggc
     1201 acaaaagccg accccgattc gccgtattgt gaagaatgtg atctcggtct tcgtcactat
     1261 ccgttcagct tcttaaatat attctttgtc attgttgtta ttgcgcttgt tttttacagc
     1321 ggctatgtat ttgcggggcg tatgggcgtt ttaaacgatg tctataaggc agataaatat
     1381 gtttcgcaga agaaaatgga ttcagccgtt accgcatatg aggatacggt ttccgcaatg
     1441 gagaaggcag gtataaaggg cgagcttgtg tataagcgcc aaataatggc aaattaccgc
     1501 ataggctacc ttcaggatat tgactctatg agcggcaata taaattcttg ggagctgtct
     1561 cttccgcact tcagaagcgt taaaaaagca atgggcgagt cggcggattt tatagccacc
     1621 gtgcgcaagg cttatacaat acttacaaag tatgatgtca acgatccttc ggatttcccg
     1681 tatgacgacg ctatggcggc tttggtgtcg cttgagagcg agccttatac cgaggataca
     1741 acggcaaata cttcgtccgc gacgacgtca tccgttaagg ccacgcagta taataaggcc
     1801 atggtatatt actatgaata ttatatatcg gtaatctgca ataaggataa cgacgaacag
     1861 ctcaaatacc ttgaaaagat taaagaagaa tatcctcagt atatttggct ttacggttcg
     1921 gccctcggcg atctttacgc aaaaacaggc aaggacgtca ccgaaatctg cgataaaatc
     1981 cgcagcaata attccgagga cccgtcgccg gatcttattg aaatagaaag cctgcgcata
     2041 aagggcgatt atgacgcggc aatagcgaaa tgtgaagaat acgcttcacc ggatgactgc
     2101 acggtcaaat atgagattta ccgtcaggag gcactttgct atatcctcaa ggacgattac
     2161 gacacggctt tagaaacggc gaaaaaggct tatgacgata acggcaattc tcttgaggtt
     2221 atagatacct tggcactttg cgctgttttg aaaaacgatg atgccacata cagcagcatg
     2281 gaatcgcttt tggacggcag cggttacagc ctgtccgata aggttaccgg cttcaaggca
     2341 ggtaccgtta cgcttgatga tattcttttg aaaggagctt atgacgtaga atgagagcag
     2401 tatatcttac agtaaaatat ataaccatcg tcggtacgtt ccttcagggc ttttttgagc
     2461 atctctcatg ccgtatgtat gatgttctga tagaggacgg cagatatttg agatcaaatg
     2521 agatgtgcgg gcatatagag cacgagctta ttaaaaagag aggcaccgca ttcggtgtgt
     2581 gcttcttccc gttccttttc aatttgctga taggtcttat cctgaccgcg gcaggctcgg
     2641 taaatatttt ctatctcggt gaatttttta tgagcgccga ttcaaaagta ccgaatttcc
     2701 cgaacttctt atttctgtgg gtcggtattt cgtgccttac caatctgttc ccgcagatag
     2761 aagacgctct taccctcaaa gagcagattt acggcaaggg caagtcaaat ctctttgtga
     2821 aaataatcgc cgcgccgata ttcgccgtgc tgtatgtcgg ctcatatctg cagagcgtcg
     2881 ggcttacgct tgtcacgtct gtggtattct cgctgtttat gccgtcgatt cttggcacgt
     2941 ttatacctca gctctacaat g
//
LOCUS       DBMDVO010000088         2929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15677_length_2929_cov_1.234088, whole genome shotgun sequence.
ACCESSION   DBMDVO010000088 DBMDVO010000000
VERSION     DBMDVO010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15677_length_2929_cov_1.234088"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="ACZPE2_02065"
     CDS             <1..1870
                     /locus_tag="ACZPE2_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318664.1"
                     /translation="SMQFENAVADVDIYKANTRTEKINTALALYAQLSDYQKSIPSVA
                     RAYSVLQKYKSIDDGITLLDQPQLAVADLKPLIEAMPAAATGGSVTGSNALPYPGFVI
                     GTDASGTEKPLIDYGAYSLTAADVQNIINWYEQSYERYTDEAIAALGAENVTKMENLY
                     KVAKRLRLLENYRDGYTSDSGTAITGIKDLNTLVASMYGETGKATDLTTDCRLMPTGQ
                     TAALENYIEKYNDLDYYAKYILTDKADKFASAPQYKNYAKAAEAVNKVLANIKTYTDD
                     ITGKTVKGGVVRLEEKYTALYNTVKGQMDNNGIQDLTAITEAVEEYNSFKGYIRQVQE
                     VNDGNTVNSSDGEALHGIIDTLRIFPNFASAVDKDSVALNKDATTGTAVFTLSNAVNY
                     PDGSYTLRVTSKNGGLKDVSGNILDYNVAMTGAIEKTVTAKALNTSPETADVANNTAS
                     AAAPKAINFTFTVTGVTVTSEDVITIELIDNATGEVAVDKTTNEPVRKTINIHFAADD
                     FFEVTIPAEIDVPWGETEAVDVSYKVTSSLDTGSKIGVSVARSASVANDTLTNAATST
                     YALPYTSQNFTSTEFTGTNEGALPAQKPSLTISGWTDAPIAEYSTTLTYTVDYTKG"
     gene            1915..2394
                     /locus_tag="ACZPE2_02070"
     CDS             1915..2394
                     /locus_tag="ACZPE2_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318665.1"
                     /translation="MKKTLSVLLSVLLLSLVTVPSFAAPATLNKENPVGSVKIFTGEG
                     KQDEYELTIPADTEIPWEATSYAIGKVTATKMWLSPGKTVKVAVTSLNGSKLVNTLDS
                     SKTIAYTLTGAEQMAFLPGDYMKSYDLSVNIAEAEWQKAASGEHTDVLTFTAEYTNA"
     assembly_gap    2472..2481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2482..2905
                     /locus_tag="ACZPE2_02075"
     CDS             <2482..2905
                     /locus_tag="ACZPE2_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318666.1"
                     /translation="ETEQTGTTIIKTSTKTGPDSPEDAAVRYTVSIPADTEIYWGTEE
                     TNLVYSVESHLKRGQALKVTVAGTGSMKTDAAAGEVYTLPYTLSGDTAFTADSPVVYG
                     NKETPAVDKNLKVTISSEDWNRAVVDVYNDTLTFTAEV"
BASE COUNT          836 a          701 c          673 g          709 t
ORIGIN      
        1 ttcaatgcag tttgaaaacg cggtagcgga tgttgatatt tataaagcga acactcgtac
       61 cgaaaagata aacacggcac ttgctcttta tgcacagctt tcggattatc aaaagtcaat
      121 accttccgtt gcaagagcat acagcgtgct tcaaaagtac aagagcatcg acgacggcat
      181 cactttgctc gatcagccgc aacttgcggt tgccgatttg aaacctttga ttgaggctat
      241 gccggctgca gcaaccggcg gttctgtaac aggcagcaac gccttacctt atcccggttt
      301 tgttatcggc accgatgcga gcggaacaga gaagccgctt atagattatg gcgcatattc
      361 tctcaccgcg gctgacgtac agaacataat caattggtat gagcagtctt atgagcgcta
      421 taccgacgag gcgattgcgg cgctcggtgc ggagaacgtc acaaaaatgg aaaaccttta
      481 taaggttgca aaacgtttga gacttctcga aaattaccgt gacggctaca cgagtgactc
      541 cggtaccgct ataaccggta ttaaagacct taacactctt gttgcttcaa tgtacggcga
      601 gaccggtaaa gcgaccgacc ttacgaccga ctgcaggctt atgcctaccg gtcagaccgc
      661 ggcgcttgaa aactacatcg aaaagtataa cgaccttgat tattacgcta agtatatctt
      721 aactgataag gccgacaaat ttgcaagtgc tcctcagtat aagaattatg ctaaggcggc
      781 cgaggcggtt aacaaggtgc ttgccaatat caaaacttac accgacgaca tcaccggtaa
      841 gacggttaag ggcggcgttg ttagacttga agaaaagtac acggctcttt acaataccgt
      901 taagggtcaa atggacaata acggtattca ggaccttacc gcaataacgg aagcggttga
      961 agagtacaat tcgtttaagg gctatataag gcaggttcaa gaggttaacg acggtaacac
     1021 cgttaattcc tcggacggcg aagctctcca cggtattata gataccctcc gtattttccc
     1081 gaactttgct tcggcggtag acaaagacag cgtcgctctc aataaggatg caacaacggg
     1141 aaccgcagtc tttactcttt caaatgccgt taactatccg gacggaagct acacacttcg
     1201 tgtaacctct aaaaacggag gtcttaagga cgtttcaggc aacatcctcg attacaacgt
     1261 tgcaatgacg ggtgcgattg aaaagaccgt aacggcaaaa gctcttaaca cttctcccga
     1321 aactgcggat gtcgcaaata acaccgcttc ggcggcagct cccaaggcaa taaactttac
     1381 gtttaccgtc acgggcgtta ccgtaacgag tgaagatgtt attacaatag agcttatcga
     1441 taatgctacc ggtgaggttg ctgttgacaa gacaacaaac gagcctgtca gaaaaacaat
     1501 caatattcat tttgccgcag acgacttctt tgaggttaca attcctgcgg aaatagatgt
     1561 tccttggggc gagacggaag ccgttgatgt ttcctataag gttacgtctt cacttgatac
     1621 gggcagcaag attggtgttt ccgtagcacg cagcgcttct gtcgcaaatg acactcttac
     1681 caatgcagca acatcgacat acgctcttcc gtatacttca cagaacttta cttccacaga
     1741 atttacaggt acaaatgagg gagcgcttcc cgctcaaaaa ccgtcattga cgatttccgg
     1801 ttggacggac gctccgatag ccgagtattc gactactctt acttataccg tagattacac
     1861 taaaggttaa ttaacaaatt aaatgcttta tcaagaccat aaggaggtgg ttctatgaag
     1921 aaaacattat cagttttgct ctcagtgctt ttattgtcgc tcgtgactgt tccgtctttc
     1981 gcggctccgg ctacgctgaa caaagagaac cccgtaggct ccgtaaagat ttttaccggc
     2041 gagggcaaac aggatgagta tgagcttaca attcctgccg ataccgaaat accgtgggaa
     2101 gctacttctt acgcaatagg caaggttacg gcaactaaaa tgtggctgtc tcccggcaag
     2161 accgttaagg tggctgttac gtcgctcaac ggctcaaagc ttgtcaacac tttggacagc
     2221 tcaaagacta tcgcctacac gcttacaggc gcagagcaaa tggcgttttt accgggcgat
     2281 tatatgaagt cttatgacct ctccgtgaac atcgcagagg ctgaatggca aaaagccgct
     2341 tcgggtgagc atacagatgt tcttaccttt acggcagaat atactaacgc gtaataaata
     2401 taaggagata aatcatgaaa aagcttattt ctatcgcact cgcagcggct atgctcgcag
     2461 ctgtttcggt tnnnnnnnnn nggaaacaga gcagaccggc acaaccataa ttaagacatc
     2521 cacaaaaaca ggccccgatt cgcctgagga tgctgctgtc agatacaccg tttctattcc
     2581 ggcagataca gaaatttact ggggcaccga ggagacaaac cttgtttact ctgttgagtc
     2641 tcacctcaag agaggccagg ctctcaaggt taccgtagca ggcacaggct ctatgaagac
     2701 cgatgccgca gcaggcgagg tttacactct tccctatact ctttcgggcg acaccgcttt
     2761 cacggcagac agccccgtag tttacggcaa caaggaaact cccgccgttg acaaaaacct
     2821 caaggttaca ataagcagcg aggactggaa cagagctgtt gttgatgttt acaacgacac
     2881 ccttaccttt accgctgagg tttaattatt gaaaggagac tactataat
//
LOCUS       DBMDVO010000089         2923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15725_length_2923_cov_1.063430, whole genome shotgun sequence.
ACCESSION   DBMDVO010000089 DBMDVO010000000
VERSION     DBMDVO010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15725_length_2923_cov_1.063430"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="ACZPE2_02080"
     CDS             complement(<1..406)
                     /locus_tag="ACZPE2_02080"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1318667.1"
                     /translation="MKARNLIDLEDISLEDIDEIIHLAGMIKANPSIFTDACHGKIMA
                     TLFYEPSTRTQMSFKTAMLRLGGEVIGFDNPSNASVSKGESLKDTVRVVSNYADILVM
                     RNPIEGSAYAASLYSKSPVINAGDGGHLHPTQT"
     gene            complement(548..1264)
                     /locus_tag="ACZPE2_02085"
     CDS             complement(548..1264)
                     /locus_tag="ACZPE2_02085"
                     /inference="COORDINATES: protein motif:HMM:NF020296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR1318668.1"
                     /translation="MELSKVQWNGRAYAEFRDYLLSFSDEKYRAFHSGLCKTSRYPII
                     GVRLPIMQKISSEIVKGKPLYFLMAASVQSYEEIMIRGLVTAKLKMPLAEKFVYIEEF
                     LPYIDNWAVCDSFCTALKPKKNELYMLFEFCKEHAFSQNTYETRTAVVLMMRYLLTDE
                     FGEDVISILENIDCTEYYVGMAVAWALSESFIKFRERTLEVFSSGNVDAATQNRAIQK
                     IRDSYRVSDSDKAFVNSLKR"
     gene            1421..>2923
                     /locus_tag="ACZPE2_02090"
     CDS             1421..>2923
                     /locus_tag="ACZPE2_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1318669.1"
                     /translation="MIYKLNRDNYRDFSALNINRLPGRAYSIPFRELSRLKKTDCLTE
                     RYKSDMVTVLSGEWDFKYYETQNVLPYEMDTERIRFDKIQVPSTWQRTGYREPVYLNT
                     RYEFHLFPPELPKEVAVGVYRKKITVDDKTKTHIISFLGFSACLELYVNGEFCGYGEG
                     SHNSAEFDISNMLISGENELLCVVYRWSTGTYLECQDMFRENGIFRDVLLYTYDKAYI
                     NDYEVKTRLNGGREYDLDIDVFLEGEAAGKKVSAELFSGRKKICSDEKDADVCVKFSF
                     SALDVKEWTAETPNVYELYLTLKDGDDTLCTVRNYTGFKHIEILGDVFKFNCEKIKFK
                     GVNHHDSNAKTGYVMSAADIKKDLVLMKELNVNSIRTSHYPPDPMLITLADIMGFYII
                     DEADIETHGCGSLGKYNLYKPNFISNDRSWAPRYVDRVSRMYFRDRNHPSITMWSLGN
                     ESGGYKCQDKCYEFLKSVCPEIPVHYEGAIRTKRVGYDVISEMYTDIENVI"
BASE COUNT          872 a          641 c          616 g          794 t
ORIGIN      
        1 aggtctgagt gggatggaga tgtccgccgt cacctgcgtt tatgacggga cttttggaat
       61 agagcgacgc tgcataagcg gagccttcta tgggattacg cataaccaaa atgtcggcat
      121 aattgctgac cacccttacg gtatccttca agctctcgcc ctttgacacg gacgcattag
      181 acggattgtc aaaccctata acctcgccgc cgagacgaag catggcggtt ttaaacgaca
      241 tctgagttct tgttgacggc tcataaaaaa gcgtcgccat tattttgccg tggcaggcgt
      301 cggtaaaaat cgacggattt gcctttatca taccggcaag gtgaattatc tcgtcaatat
      361 cctcaaggct gatgtcctca aggtcaataa gattgcgtgc cttcataaat taccattcct
      421 ttatttttta cagtaacggt atgcttccgt tttgtaaaca ttataatctt ttttgtattc
      481 ttcttcaata gcggcaatga aaaattgaga gtttatacaa attttaccga aacacgtata
      541 ttgtttttta ccttttcaag ctgtttacaa atgctttatc gctgtcggac acacggtatg
      601 agtctctgat tttttgtatc gccctgtttt gagttgcggc gtctacattc cccgatgaaa
      661 atacttcaag cgttctctct cggaatttta taaagctctc cgaaagagcc caggcaaccg
      721 ccataccgac gtagtattct gtacaatcga tattttccaa aatcgaaatg acgtcctctc
      781 cgaattcgtc cgttaaaaga tagcgcatca tcaaaacaac agctgtacga gtctcatacg
      841 tattctgtga aaaagcgtgc tccttacaga attcaaaaag catataaagc tcgttcttct
      901 taggcttcag cgccgtgcaa aacgaatcac ataccgccca attatcaata tacggcaaaa
      961 actcctctat atatacgaat ttttcggcta acggcatttt gagcttggcc gtgacaagtc
     1021 ctcttatcat aatctcttca tacgactgca cagaggccgc cataagaaaa taaagcggct
     1081 tgccctttac tatttcggag gaaatctttt gcattatcgg gagccttact ccgattatcg
     1141 gatatctcga tgtcttgcag agcccgctgt gaaaagctct gtatttttca tctgaaaacg
     1201 acaatagata atctctgaat tccgcatatg ccctgccgtt ccactgtacc ttagaaagct
     1261 ccataaaatc cacctgcgtc ctttcggctt atttttacgc aaactgcata aaaaagcgct
     1321 tgatactttt accttaaaaa gtataatata tcgcaattta attgtaaatg atttaattct
     1381 gtgttaaact gtttgcagat tgcagaaagg ggaaaatttt gtgatataca agctaaacag
     1441 agataattac agagattttt cggcactgaa cataaacaga ctgcccggaa gagcttactc
     1501 gattcccttc agagagcttt cgcgtctcaa aaagaccgat tgtctgaccg aacgctataa
     1561 gagcgatatg gttacggttc tctcgggaga atgggatttt aaatattacg aaacgcagaa
     1621 tgttttaccg tatgaaatgg acacggagcg tatccgtttt gataagatac aggttccgtc
     1681 cacctggcag agaaccgggt acagagagcc tgtatattta aatacaagat acgaatttca
     1741 tctttttccg ccggagcttc cgaaagaggt tgccgtaggc gtatacagga aaaagataac
     1801 cgttgacgac aaaaccaaaa ctcatatcat ttcattttta ggcttcagcg cgtgccttga
     1861 gctgtatgta aacggtgagt tttgcggcta cggcgagggc tcacacaaca gtgcggaatt
     1921 tgacatttca aatatgctga tttccggtga aaacgaacta ctctgcgttg tttaccgttg
     1981 gtccacgggg acatatctcg aatgtcagga tatgttcaga gaaaacggaa ttttccgaga
     2041 cgttctgctt tacacatatg acaaagcata tataaacgat tacgaggtta aaacacgctt
     2101 aaacggcgga agagaatacg acctcgatat agacgtattc cttgagggcg aagccgcagg
     2161 aaaaaaagtt tcggcagagc ttttctcagg cagaaaaaag atatgctccg acgaaaagga
     2221 tgccgatgtc tgcgtcaaat tttccttctc ggcactcgac gtaaaggaat ggactgcgga
     2281 aacgccgaat gtttacgagc tttatttgac gcttaaggac ggtgacgata cactatgtac
     2341 cgtaagaaat tataccggct tcaagcatat agaaattctc ggcgacgtat ttaaattcaa
     2401 ctgcgaaaaa ataaaattca agggtgtaaa ccatcacgac agcaatgcaa aaaccggcta
     2461 cgtgatgagt gccgcggaca taaaaaaaga ccttgttctt atgaaagagc ttaacgtaaa
     2521 ctcgataaga acgtcgcact atccgcctga cccgatgctc attacgcttg cggacattat
     2581 gggattttac ataatagacg aggccgacat cgagacacac ggctgcggaa gtctcggcaa
     2641 atacaacctc tataagccta atttcataag caacgaccgc tcttgggcac cgagatacgt
     2701 tgacagagtg agccgtatgt atttcagaga cagaaaccac ccgtcaatca cgatgtggtc
     2761 tctcggcaat gaatccggcg gatacaaatg ccaggacaaa tgctatgaat ttttgaaatc
     2821 tgtatgtccg gaaatacccg tacactatga gggcgctatc cgcacaaaga gagtcgggta
     2881 tgacgtaata tcggaaatgt ataccgacat agaaaacgtt ata
//
LOCUS       DBMDVO010000090         2920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15751_length_2920_cov_1.402625, whole genome shotgun sequence.
ACCESSION   DBMDVO010000090 DBMDVO010000000
VERSION     DBMDVO010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15751_length_2920_cov_1.402625"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="recF"
                     /locus_tag="ACZPE2_02095"
     CDS             <1..1029
                     /gene="recF"
                     /locus_tag="ACZPE2_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1318670.1"
                     /translation="SESVNVIYGENAQGKTNLLESIWLFTGCKSFRSKRDRELVRFDA
                     SYAKNEMTFFAKGREQSAVIFIDKKRTAMKNGVTLRSPAELIGEFYAVIFSPSHLSLV
                     KDGPVFRRKFLDTALCEIKPYYAKALAKYTHTLFQRNALLKDIQFHSELYDTLDAWDD
                     KLSSYGAAVISERIKYTALLSEFSAKIYDGLSGGKEKMSVEYTDNAKVYSEDPYEIKD
                     FLLNRLRETRRDDIYNKITSIGPHRDDLDIKIDGLSARSFGSQGQQRSSALSLKLSES
                     EIIKEITGDEPVILLDDVMSELDLTRQDYILNNISGRQVFITCCDPNTVLRLCEGKTF
                     HIKNGGVI"
     gene            1029..1280
                     /gene="remB"
                     /locus_tag="ACZPE2_02100"
     CDS             1029..1280
                     /gene="remB"
                     /locus_tag="ACZPE2_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix regulator RemB"
                     /protein_id="HIR1318671.1"
                     /translation="MYILISQDKAITTDGILGIFDLDNTTVSKASREYINYAEKSGEC
                     ETVSFDLPKSFIVKVTGGKRKVYISPFSTSTVFKRLKQD"
     gene            1331..>2920
                     /locus_tag="ACZPE2_02105"
     CDS             1331..>2920
                     /locus_tag="ACZPE2_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR1318672.1"
                     /translation="MFSENNENINEEKNDEVMDTVVTEEYGAEQIQVLKGLEAVRKRP
                     GMYIGSTGPRGLHHLVYEIVDNSIDEALAGYCTHIEVEILPGNVITVRDNGRGIPVDI
                     NEEEGLPAVTVVLTVLHAGGKFGGSGYKVSGGLHGVGSSVVNALSEWFEIEVSRQGHI
                     YHQRFERGDVKSDLEIIGDTNETGTFIKFKADPEIFTETTEYDFETLQKRLREQAFLN
                     AGLSISLTDKRDEDNILSEVYCYEGGIRSFVDYINKSRGLTPLHEDIMHFSVVDGDRS
                     AEVAMSYNDGYNEIILSFANDVRTIDGGTHEMGFKTALTRVFNDYGKKFNLIKDSDKK
                     LSGDDVREGLTAVISVKLTEAQFEGQTKGKLGNTEITGLVSKMLYDKLMTYLEENPST
                     ARTILNKSLDASRAREAARKARDNARRKSALESNSLPGKLADCTDKNPENTELYIVEG
                     DSAGGSAKGGRDRNTQAILPLWGKMLNVEKARVDKVIGNEKLTPVILALGTGIGDDFD
                     ITKLRYHKIVLMADADVDGAHI"
BASE COUNT          882 a          631 c          748 g          659 t
ORIGIN      
        1 tccgagagcg taaacgtcat ttacggagaa aacgctcagg gaaaaaccaa tttgcttgag
       61 agtatatggc tctttacggg atgcaagagc ttccgttcaa aaagagacag ggagcttgtg
      121 aggtttgatg cgtcatacgc aaaaaatgag atgacctttt tcgcaaaggg acgcgagcag
      181 tctgcggtta tttttataga caaaaagcgt accgccatga aaaacggtgt aacgctgcgt
      241 tcccctgcgg agcttatagg cgagttttat gcggttatat tttctccgtc tcatctgtcg
      301 cttgtaaagg acgggccggt tttcagaaga aaatttcttg atacggctct gtgcgagata
      361 aagccgtatt acgccaaggc actcgcaaaa tacacgcaca cactttttca gagaaatgcg
      421 cttttaaagg acattcagtt tcacagcgag ctttacgata cacttgacgc ctgggacgat
      481 aagctttcgt cttacggtgc ggcggtaata tcggagcgaa ttaaatacac ggctctcttg
      541 agtgaatttt ccgcaaaaat atacgacgga ctgtcgggcg gcaaggaaaa aatgagcgtt
      601 gaatataccg acaacgcaaa ggtatattcg gaagacccgt atgaaataaa ggatttcctc
      661 ttaaacagac tcagagagac gcggcgcgac gatatttaca acaagataac gtcgataggt
      721 cctcacagag atgaccttga cattaaaata gacggcctgt cggcacgcag cttcggctca
      781 caggggcagc agcgctcctc ggcactgtct ttaaagctca gcgaatcgga aattataaag
      841 gaaataacgg gcgatgagcc ggtaatactt cttgacgacg tgatgagcga gcttgattta
      901 acaaggcagg attatatttt gaacaacata agcggaaggc aggtttttat aacctgctgc
      961 gacccgaata ccgttttgcg gctgtgcgag ggcaagacct tccacataaa aaacggcgga
     1021 gtgatataat gtatattttg ataagtcagg acaaggcaat tacaacggac ggaatactcg
     1081 gcatatttga ccttgacaac actaccgtga gcaaggcttc gcgcgaatat ataaattacg
     1141 cggaaaaaag cggagagtgt gaaacggtgt cgttcgattt gccgaaatcc tttatagtaa
     1201 aggtaacggg cggcaaaaga aaagtatata tatcaccgtt ttcaacaagc actgttttca
     1261 aaagattaaa gcaagattaa aaatttaagt aaattttatt cggattttgt ataactgacg
     1321 gaggttttgt atgttcagcg aaaacaacga aaacataaac gaggaaaaga acgacgaggt
     1381 aatggatacc gtcgttaccg aggaatacgg cgcggagcag attcaggtat taaagggtct
     1441 tgaggccgta agaaaaagac ccggtatgta cataggctcg acgggaccga gaggtcttca
     1501 ccaccttgta tatgagattg tcgataactc aatagacgag gctcttgcgg gctactgcac
     1561 tcatatcgag gttgaaatac tccccggaaa cgtgattacc gtcagagata acggacgagg
     1621 aatccccgtt gacatcaacg aggaggaggg tctgcctgcc gtaacggtgg ttttaacggt
     1681 tctccacgcc ggcggtaaat tcggcggcag cggctacaag gtttcgggcg gtctgcacgg
     1741 cgtcggttca tcggtagtta acgctctttc cgagtggttt gaaatcgagg taagcaggca
     1801 gggtcacata tatcaccagc gttttgagcg cggcgacgta aagagcgacc ttgaaataat
     1861 cggcgacaca aacgagacgg gtacatttat taaattcaag gccgaccccg aaatattcac
     1921 cgaaactacg gaatatgatt ttgagacact tcaaaagaga ctcagagagc aggcgttttt
     1981 aaatgccgga ctttcaatct cgctcacgga caagcgtgat gaggacaaca tattaagcga
     2041 ggtatactgc tatgagggcg gtattcgcag ctttgtggat tatatcaaca aaagccgcgg
     2101 gcttacccct cttcacgaag atataatgca tttttcggtt gttgacggcg accgcagcgc
     2161 ggaagtcgca atgtcttaca acgacggcta caacgagata atattaagct ttgcaaacga
     2221 tgtccgcaca atagacggcg gtactcacga aatgggcttt aaaacggctc ttacgagagt
     2281 gttcaacgac tacggcaaaa agtttaatct cataaaggac agcgacaaaa agctctccgg
     2341 cgacgacgta agagagggcc ttaccgcggt cataagcgta aagcttaccg aggcacagtt
     2401 tgagggtcag accaagggca agctcggaaa caccgagata accggtcttg tttcaaaaat
     2461 gctctatgat aagcttatga cctatctcga agaaaatccc tccacggcaa gaaccatact
     2521 taataagtcg ctcgacgctt caagggcgcg cgaggcggca agaaaggcga gggacaatgc
     2581 gcgcagaaaa tctgcgcttg agagcaactc tctccccggc aagcttgccg attgcaccga
     2641 taaaaatccc gaaaataccg agctttacat cgtcgagggt gattccgcag gcggctccgc
     2701 aaagggagga agagacagaa acacccaggc aattctccct ctttggggca agatgctcaa
     2761 tgttgaaaag gcgagagttg acaaggtaat aggcaatgaa aaattaacgc ccgtaatttt
     2821 ggctctcggt accggcatag gcgacgactt cgacatcaca aagctgcgtt atcacaagat
     2881 agttttaatg gcggatgccg atgttgacgg cgcgcacatc
//
LOCUS       DBMDVO010000091         2918 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15771_length_2918_cov_1.062478, whole genome shotgun sequence.
ACCESSION   DBMDVO010000091 DBMDVO010000000
VERSION     DBMDVO010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15771_length_2918_cov_1.062478"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /gene="iorA"
                     /locus_tag="ACZPE2_02110"
     CDS             <1..1572
                     /gene="iorA"
                     /locus_tag="ACZPE2_02110"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR1318673.1"
                     /translation="VAMEVAVGSSIAGARSFCAMKHVGLNVAADPLFTASYTGVNAGM
                     VIAVADDPGMHSSQNEQDSRHYAKAAKLLMLEPSDSEECLEYAKAAYELSEKFDTPVI
                     LRLTTRVAHSRSIVNIGERNDIGVKDYVKNPGKYVMMPAMAKKRHIVVEDRMVAEREW
                     AETASINTVEYNDKKIGVISSGICYQYAKEALGDRVSYLKLGCVNPLPIELIKNFAAN
                     CDKVYVLEELDPFIEEHCKQIGIDVIGKDAFTLQGEYSQNLIAKVIFGETKPTMKADI
                     DIPARPPVLCAGCPHRGLYYALKKLKLTVSGDIGCYTLGALPPIGMMDTCVCMGASVS
                     ALHGRNKADENNCKNSVAVIGDSTFIHSGITGLIDIAYNQSNSTVIILDNSITGMTGH
                     QDNPTTGKTIKGDPTTAVSLELLAKAVGIDRVRVVDPYNLKECEDVIREEVAADEPSV
                     VISRRPCALLKYVKHNPPLKINKDKCVGCKMCMKIGCPAISMRDGKSEIDFTQCVGCN
                     VCSQLCKFDAIEEGK"
     gene            1574..2152
                     /locus_tag="ACZPE2_02115"
     CDS             1574..2152
                     /locus_tag="ACZPE2_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR1318674.1"
                     /translation="METKSIMIVGVGGQGTLLASRILGAALISNGFDVKVSEVHGMSQ
                     RGGSVVTYVKYGSKVNSPIVNEGEADLILAFEQLEAARWLQYLKKDGTLIINTQKIDP
                     MSVIIGNSVYPDGVLDAINKAGAKTTNLDALSLAVSAGSAKAVNVVLIGVMASHMDLN
                     RDVWINAIKNTVPEKFLEMNLKAFDLGYNYNK"
     gene            2173..>2918
                     /locus_tag="ACZPE2_02120"
     CDS             2173..>2918
                     /locus_tag="ACZPE2_02120"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR1318675.1"
                     /translation="MQNYFTPDIETASRDYLTAIQSARLIKMVENCYKHVPFYKQKFD
                     EIGLLPGDIKSIADITKLPFTIKQDLRDNYPFGLFAVPKEKLVRVHASSGTTGKQTVV
                     GYTKHDISIWSESAARALSAAGATKGDYIHISYGYGLFTGGLGLHYAAEKLGATAIPV
                     SSGNTNRQMQILQDFGSDIICCTPSYAMYIGESLKAAGVDSTKLPLRVGIFGAEAWSE
                     NMRKEIERSLNIKAYDIYGLSEIAGPGVAF"
BASE COUNT          853 a          581 c          689 g          795 t
ORIGIN      
        1 gtagccatgg aggttgctgt aggttcgtct attgccggcg cacgttcttt ctgcgctatg
       61 aaacacgttg ggttaaatgt agctgccgat ccgctgttta ccgcttcata tacaggtgtt
      121 aatgcaggta tggttattgc cgttgccgat gatccgggga tgcattcctc acagaatgaa
      181 caggacagcc gccactatgc taaggcagct aagttattga tgcttgagcc gtctgactcc
      241 gaggaatgtc tcgagtatgc caaagcggct tatgagcttt cggaaaagtt tgatactccc
      301 gtcatattaa gattgacaac aagagtcgcc cattcaagaa gtatagttaa tatcggcgaa
      361 agaaacgata taggcgttaa ggattatgtg aaaaatccgg gtaaatatgt tatgatgcct
      421 gccatggcaa agaaaagaca tattgttgta gaggacagaa tggttgcaga gcgcgaatgg
      481 gctgaaaccg cctctataaa taccgttgaa tataacgata agaaaatcgg tgttatctct
      541 tccggtattt gctatcaata tgcaaaagag gcattgggcg accgcgtttc gtatcttaaa
      601 ctcggctgtg taaatccgct tccgattgaa cttattaaga actttgccgc taactgtgac
      661 aaggtctatg ttcttgaaga gcttgacccg tttattgagg agcattgcaa gcagatagga
      721 atagatgtta taggtaaaga cgctttcaca ttacagggcg aatactcaca aaaccttata
      781 gcaaaagtca ttttcggcga aacaaagccg actatgaaag cggatattga tattcccgca
      841 cgtccgcccg tgctttgcgc cggttgtcct catagaggtc tttattatgc gctgaaaaaa
      901 ttaaaattga cggtaagcgg cgatataggc tgctatactc tcggtgcact tccgcctatc
      961 ggtatgatgg atacctgtgt ttgtatgggt gcttcggtat ctgcacttca cggcaggaat
     1021 aaggctgatg aaaacaactg caagaactct gtagcggtta tcggcgattc aacgtttatt
     1081 cattccggta ttacaggact tattgatatt gcttataatc aaagtaactc gaccgttatc
     1141 atacttgata acagcattac gggtatgacc ggtcaccagg acaatccgac tacaggaaag
     1201 acaattaagg gcgacccaac taccgcagtc agcttagagc ttcttgcaaa ggctgtcggt
     1261 atcgacagag tcagagtagt agacccgtat aaccttaaag agtgtgaaga tgtaatccgt
     1321 gaagaagttg ccgcagacga gccgtcggtt gtaatttcga gaagaccttg tgctctttta
     1381 aaatatgtta aacacaatcc tccgcttaag ataaacaaag ataaatgcgt tggttgtaaa
     1441 atgtgcatga agattggctg tccggctatc agcatgagag acggaaagtc ggagattgat
     1501 tttacacaat gtgtcggctg taatgtatgc agtcagctct gcaaatttga cgcaatagag
     1561 gagggcaaat aacatggaaa caaaaagtat tatgattgtc ggagtcggcg gacagggcac
     1621 tcttcttgca agcagaatac ttggcgcggc gcttataagt aacggcttcg acgttaaggt
     1681 ctcagaagta cacggtatga gtcagcgcgg cggatcggtt gttacttatg taaaatacgg
     1741 ttcaaaagtt aattcaccca tagttaatga gggcgaagcc gatttgattc ttgcatttga
     1801 gcagcttgaa gccgcaagat ggcttcagta tcttaaaaaa gacggaacac ttataataaa
     1861 cacgcaaaaa atcgacccga tgtcggtaat aatcggcaat tcggtttatc ctgacggtgt
     1921 acttgatgct attaataaag caggagcaaa aactacaaat cttgatgcac tttctttggc
     1981 tgtctcggca ggctctgcca aagccgtaaa tgtcgttctt atcggtgtta tggcctcaca
     2041 tatggatttg aatcgtgatg tttggattaa cgctattaag aatactgtac ctgagaaatt
     2101 ccttgagatg aatctcaaag catttgacct cggttacaat tataataagt aagaaagaac
     2161 ggagatattc aaatgcaaaa ctatttcacc ccggacattg aaaccgcttc aagagattat
     2221 cttactgcta tacagtctgc aagactgatt aaaatggttg aaaactgcta caagcacgtg
     2281 ccattttata agcagaagtt tgatgagatc ggtcttctgc ccggtgatat taaatcaata
     2341 gcggatataa ctaagctgcc ttttacgatc aaacaggatc ttcgcgacaa ttatccgttc
     2401 ggtctttttg ccgttccaaa agaaaagctt gtgcgagttc atgcttcttc gggaacaaca
     2461 ggcaaacaga ctgttgtggg ctatactaag catgatattt ccatttggag cgaaagtgct
     2521 gcacgagctt tatcggcagc aggtgctaca aaaggagatt atatacatat atcttatggc
     2581 tacggccttt ttacgggcgg cctcggactg cattatgccg ctgaaaaact cggagcaacc
     2641 gccattcccg tgtcatccgg caataccaac agacaaatgc aaattttgca ggatttcggt
     2701 tcagacatta tttgctgtac tccgtcgtat gccatgtata tcggcgaatc actgaaagct
     2761 gccggtgtcg attccacgaa gctaccttta agagtgggaa ttttcggtgc ggaagcatgg
     2821 agtgaaaata tgcgcaagga aatcgaaagg tcacttaata tcaaagctta tgatatttac
     2881 ggtctttcag aaattgcagg acccggcgtc gcttttga
//
LOCUS       DBMDVO010000092         2905 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15872_length_2905_cov_1.185449, whole genome shotgun sequence.
ACCESSION   DBMDVO010000092 DBMDVO010000000
VERSION     DBMDVO010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_2905_cov_1.185449"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(192..434)
                     /locus_tag="ACZPE2_02125"
     CDS             complement(192..434)
                     /locus_tag="ACZPE2_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318676.1"
                     /translation="MSAFTAISSMIELAVIIFLIYGFYKEDKFIAFEQKVKMIIVVNY
                     RRYKRKKRIEQMRKNREFKLVDGGRKGAERGSFHVA"
     gene            766..2037
                     /gene="obgE"
                     /locus_tag="ACZPE2_02130"
     CDS             766..2037
                     /gene="obgE"
                     /locus_tag="ACZPE2_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1318677.1"
                     /translation="MFVDIAKINIKAGNGGNGAVAFHREKYVAAGGPDGGDGGRGGNI
                     VFQADDSLATLSDFRYKRKYKAPSGEDGRGARQNGKKGQDLIIRVPRGTVIREVTSGT
                     IMADMSTDEPFIAAKGGKGGWGNSHFATSTRQTPRFAKSGAPGEEWDISLELKLIADV
                     GLIGFPNVGKSSLLSVVSEAKPIIGDYHFTTIIPVLGVVTMGPEQSFVMADIPGLIEG
                     AADGVGLGHEFLKHIERCRMLVHVVDVAGSEGRDPKEDFEKINEELVKFNPELAKCPQ
                     IVAGNKIDLATDEQLEDFKSFIEKKGLPYFPIVAPIKYGTKELINAVAEKLSTLPPVK
                     KYEAEEIPLSVLESKKNNGFKVTVNDGVYSVEADWLYRILSKTDLDDYASLQYFQTVL
                     KSSGIIDELVKQGIQEGDTVSIYDLEFDYIP"
     gene            2067..>2905
                     /locus_tag="ACZPE2_02135"
     CDS             2067..>2905
                     /locus_tag="ACZPE2_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1318678.1"
                     /translation="MFAKIISVGLFGMEAYMTGVEADISGGMPRFDVVGLPDTAVSES
                     RERVRSAIKNNGFDFPPSRITINLAPADIKKEGPIYDLPILIAILKSTGQLSCDTDSS
                     AFLGELSLNGEARRINGVLPMVIKAQRCGVKEIYIPAPNAAEGAVVRGITVYPVRNVG
                     QLLSHLTGGEKITPVSESDFKSYSLPDNLPDFSDVKGQHEAKRALEVAAAGGHNVMMI
                     GPPGSGKSMLAKRIPSILPDMTFEESLETTNIYSVAGVLPKGISLIKARPFRSPHHTV
                     SPAG"
BASE COUNT          822 a          643 c          703 g          737 t
ORIGIN      
        1 cgcgaaaaga tgacgaagtt gcacaaacac agtgctctgt ttttgtgcac ttttgaaaag
       61 ccgttgattt tgtcgtttta tgtactaaaa tccatacggc gtttccgcag tttaaagact
      121 acgaaaaaag caaggagccg cggataatac ccacggcttc ttgctttaag actttatata
      181 gaaatggaag attaagctac gtgaaagctg cctctttcgg caccctttct gccgccgtca
      241 acaagcttga attcacggtt tttacgcatt tgctcaatac gcttttttct cttgtatctg
      301 cggtaattga caactataat catcttgacc ttttgctcaa aggcgatgaa cttatcctcc
      361 ttataaaatc cgtatataag aaagataata actgcgagct ctatcataga agaaatggct
      421 gtgaatgctg acatattcag gacctccggt tctttagaac atttgtttgt gtttcattac
      481 acccttatta taccaacaat ttatattttg tcaatacaaa tgttcggatt ttttcggtat
      541 ataagtccta aaattaggac atttgcgttg cagagctaat acttttttaa ttccaaacgc
      601 ataataccac aacatattgt gttagtcaat actttaggca caacttatgc tatttataca
      661 taaaacgacc aaataattgt ttgttcggta tttattatgg ataatattta tggtacaata
      721 atacggtcaa atatatgtaa tttcagtaag gaacggtaaa aagaaatgtt cgtagatata
      781 gctaaaataa atattaaagc aggcaacggc ggcaacggtg cggttgcttt tcacagggag
      841 aaatatgtgg ctgcgggcgg tcccgacggc ggcgacggcg gaagaggcgg caacatagta
      901 tttcaggcag atgacagtct tgccacatta tccgatttca gatacaaaag aaaatataag
      961 gctccgagcg gtgaggacgg cagaggcgca aggcaaaacg gcaaaaaagg tcaggacctt
     1021 ataatacgtg ttccgcgcgg cacggtaata cgcgaagtaa caagcggaac gattatggcg
     1081 gatatgtcaa ccgacgagcc gtttatagct gcgaagggcg gcaagggcgg atggggcaat
     1141 tctcattttg ccacctctac acgccaaact ccgcgctttg cgaaaagcgg cgctccgggc
     1201 gaggagtggg atatttcgct tgaactgaag ctcattgccg acgttggtct tatcggcttt
     1261 ccgaatgtag gtaaatcctc gcttttgtct gtggtgagtg aggcgaagcc cattataggc
     1321 gattatcatt tcacaactat aatacccgtt ctcggcgttg tcaccatggg tcccgagcag
     1381 agctttgtaa tggcggatat tccgggtctt atagagggag ctgcggacgg tgtgggtctc
     1441 ggtcacgaat ttttaaagca tatagaacgc tgtcgtatgc tggtgcacgt agttgacgtc
     1501 gccggcagcg agggaagaga cccgaaggag gatttcgaaa agataaacga ggagcttgta
     1561 aagttcaatc ccgagcttgc aaaatgtccg cagatagttg ccggcaacaa aatagacctt
     1621 gcaaccgacg agcagcttga ggatttcaag agcttcattg agaaaaaggg acttccctac
     1681 ttcccgatag tcgcaccgat aaaatacggc acaaaggagc ttataaatgc tgttgcggaa
     1741 aagctctcta ccctgccgcc cgtaaaaaaa tacgaggcgg aggaaatacc gctttcggtg
     1801 cttgaaagca aaaagaacaa cggctttaag gttaccgtga atgacggcgt atattctgtt
     1861 gaggcggact ggctttacag aatactttca aagaccgacc ttgacgacta cgcgtcgctt
     1921 cagtattttc aaacggttct aaagtcgagc ggaataatcg acgagcttgt aaagcaggga
     1981 attcaagagg gagacaccgt ttcgatttac gatctcgaat tcgactatat accgtaatta
     2041 ccgcggtatc aaaaaggggt atataaatgt ttgctaaaat cataagcgtc ggtcttttcg
     2101 gcatggaagc ctatatgacc ggcgtagaag cggatatttc gggcggtatg ccgcgctttg
     2161 acgttgtagg acttcccgat acggcagtca gcgagagccg cgaaagagtc cgctccgcca
     2221 ttaaaaataa cggatttgac tttccgccgt cacgcattac aataaatctc gcaccggcgg
     2281 acataaaaaa agaggggccg atttacgacc tgcccattct gatagcaata cttaaatcca
     2341 cggggcaact atcctgcgat accgacagct cggctttttt gggagagctg tcgttaaacg
     2401 gcgaggcgcg cagaattaac ggcgttttgc cgatggttat taaagcacag cgttgcggtg
     2461 ttaaagaaat atatattccc gctccgaacg ctgccgaggg tgctgttgtg cgcggcataa
     2521 ccgtatatcc cgtaagaaac gtcgggcagt tgctgtcaca tctcacgggc ggagaaaaaa
     2581 taacacccgt ttccgaatcc gatttcaaat cttacagcct gcccgataat ctgccggatt
     2641 tctccgacgt caagggtcag cacgaagcta aaagagcctt agaagttgcc gccgcaggcg
     2701 gacacaatgt tatgatgata gggcctccgg gctccggcaa gagtatgctc gcgaaacgca
     2761 taccgtcgat cttgcccgat atgacatttg aagaatcgct tgaaacaacc aacatatact
     2821 cggtagcggg agttttgccc aagggaatat cccttataaa agcgcgtcct ttccgctcgc
     2881 cgcaccacac cgtctcccct gccgg
//
LOCUS       DBMDVO010000093         2903 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15889_length_2903_cov_1.002498, whole genome shotgun sequence.
ACCESSION   DBMDVO010000093 DBMDVO010000000
VERSION     DBMDVO010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_2903_cov_1.002498"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2270
                     /locus_tag="ACZPE2_02140"
     CDS             <1..2270
                     /locus_tag="ACZPE2_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012361.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1318679.1"
                     /translation="PAQSGIDHKIVAWDDVAADEGTGIVHIAPGCGVEDFELGEKLGL
                     AKVMPIDDMGIYLDGFGWMTGKDSHTVADEVFEHLKADDKLYKVEPHEHSYPVCWRCK
                     SEVVFRLVPAWYIRTDELRPKLIEAANTVKWEPASNGKRMNDWLNNMGDWNISRKRYY
                     GMPLPFYPCDCGHVTVVGSKEELRSLAVDPKLVDELPELHRPWVDKIKIKCPHCGKEV
                     SRISEIGDVWLDAGIVPYSTFGYFRDKEEWKKNFPAEWVTEMHEQVRLWFYSQLFMSV
                     VLEGKAPYERVLTNSAVIAEDGRKFSKTGFMIKFDEAAEKIGADTVRYLYAGAPVSND
                     VRFGYNLGDEARRKLLSYWNIYTFFETYAKIDKPNFENYTPDKSAMTPTDIWLVVRTN
                     EFLKKAKALYDDYKVYMLVKEFEVFVDDISNWYIRTNRRRFWKTGDEKDKMTAYWVLY
                     YALNTATLVMSPVIPFMTEHIWQNFTRKVIPSSPISVHLCDWPKPIEGFEDDGIVEKT
                     AVSREIIATAMRLRNEQQLKVRQPLSTLYVCCDSETANKIGVFEKIILDELNIKNIKY
                     IDDVNVLEDKYLTVNFKVAGAVLKQNVNKMKQTLGSLSKDDMTKLVSAVEQGDEVSVP
                     GWDDKFAADIFSVQTKTKEGIVSAELQNDKGVVALDTVLTDELIKEGLVRDTVRQCQL
                     IRKEAGYEVEQRVKMAIESLDTAVIGALKEKTEYIKSELLADELILGGTLSDADLTKE
                     VEIGDGKVKLAVAKA"
     gene            2286..2495
                     /locus_tag="ACZPE2_02145"
     CDS             2286..2495
                     /locus_tag="ACZPE2_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1318680.1"
                     /translation="MTEINKDMTIGDILDAERDTAPFFLEMGMHCLGCPASRGETVEQ
                     ACMVHGVDPDVLVENINEFLKGKNN"
     gene            2542..>2903
                     /locus_tag="ACZPE2_02150"
     CDS             2542..>2903
                     /locus_tag="ACZPE2_02150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024255.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1318681.1"
                     /translation="MINLTPRLLKTAELITPCKKLADIGTDHAYIPVYALQNDLAETV
                     VASDINEGPLNNAAKTVKQYGFEDRVVLRLSDGLENIEETEADAVVIAGMGGELIAKI
                     IGDAPWLKNNKQIIMQPMT"
BASE COUNT          867 a          533 c          697 g          806 t
ORIGIN      
        1 ttcccgccca aagcggtatt gaccataaaa tcgttgcttg ggatgatgtt gccgcagacg
       61 aaggtaccgg catagtgcat atagctccag gttgcggcgt cgaagacttt gagcttggtg
      121 aaaaactcgg tctcgcaaaa gtaatgccca ttgatgatat gggtatatat cttgacggct
      181 tcggctggat gaccggaaaa gacagccata ccgttgcaga tgaggtgttt gagcacctta
      241 aagctgatga taagctctat aaggttgaac cgcatgagca tagctatccc gtatgctgga
      301 gatgtaaatc ggaggttgtg ttcagacttg taccggcttg gtacatcaga acagatgaat
      361 tacgtccgaa gcttattgaa gccgccaata ccgttaagtg ggagcctgca tcaaacggca
      421 aacgtatgaa cgattggtta aataatatgg gagactggaa catatcccgt aagcgttatt
      481 acggtatgcc gcttccgttt tacccttgcg attgcggtca tgtaaccgtt gtaggttcta
      541 aggaagaact tcgttctctt gctgttgacc ctaaacttgt tgatgaactg cccgagcttc
      601 acagaccttg ggtggataag attaaaatta aatgcccgca ttgcggcaaa gaggtttcgc
      661 gtatatctga aatcggtgat gtttggcttg atgccggcat tgttccttac tcaacattcg
      721 gctatttccg tgataaggaa gaatggaaaa agaacttccc tgcagagtgg gttaccgaaa
      781 tgcacgagca ggttagactt tggttttatt cacagctctt tatgagtgtt gtgcttgagg
      841 gtaaagctcc ttatgagaga gttctcacta atagtgccgt aatagccgaa gacggcagaa
      901 agttctcgaa aaccggattt atgattaaat tcgatgaggc ggctgaaaaa ataggtgctg
      961 acacagtccg ctatttatat gcaggtgctc ctgtgtcaaa cgatgttcgt tttggttata
     1021 atctcggcga tgaggcgaga agaaagctcc tttcatattg gaatatttat acgttttttg
     1081 agacatatgc aaaaatcgat aagcctaatt ttgaaaatta cacaccggac aagtctgcga
     1141 tgactcctac ggatatatgg cttgttgttc gtaccaatga attcctgaaa aaagctaaag
     1201 cgctttatga tgactataaa gtttatatgc ttgtaaaaga atttgaggta tttgttgacg
     1261 atatatcaaa ttggtatata aggacaaatc gtcgtcgctt ctggaaaacc ggcgatgaaa
     1321 aagataagat gactgcttat tgggtgcttt attatgcact taatactgca actcttgtta
     1381 tgtcacctgt tattccgttt atgacagagc atatttggca gaactttacg agaaaagtta
     1441 tcccttcgtc accgatttcc gtacatctct gcgattggcc gaagccgata gagggatttg
     1501 aggatgacgg aattgttgaa aaaacggctg tctcaagaga gataattgct accgcaatga
     1561 gacttcgcaa tgagcagcag ctcaaggttc gccagcctct ttcaacactt tatgtttgtt
     1621 gtgacagcga aacggcaaat aaaataggtg tatttgaaaa gataatactt gatgagctta
     1681 acattaagaa tattaaatat attgatgatg ttaatgttct tgaggataaa tatcttaccg
     1741 taaactttaa ggtagccggt gctgttctta agcagaatgt taacaagatg aagcaaaccc
     1801 ttggatcact ttcaaaggat gatatgacga aacttgtttc tgcagttgaa cagggagacg
     1861 aagtttccgt tcccggttgg gacgataaat ttgccgctga tatatttagt gtccagacaa
     1921 aaactaaaga gggtattgta tctgccgagc ttcagaatga taagggcgtt gtggcgttgg
     1981 ataccgtgct taccgacgaa ctcattaaag agggcttggt acgtgatacg gtaagacagt
     2041 gtcagcttat aagaaaagaa gcagggtatg aggtagagca gagagttaaa atggcaattg
     2101 agtctcttga cacagccgtt attggtgctc tcaaagaaaa gaccgaatac attaagtcgg
     2161 agcttcttgc cgatgaactt atcctcggcg gaactctttc tgacgcggat cttaccaaag
     2221 aagttgagat aggcgacggt aaagtcaaat tagcggtcgc aaaagcataa tcggaggttt
     2281 taattatgac agaaatcaat aaagatatga caatcggcga cattcttgat gccgagagag
     2341 atacagctcc ttttttcctt gaaatgggta tgcattgctt gggttgcccg gcttcgcgcg
     2401 gcgaaacagt agagcaggct tgtatggttc acggtgttga cccggacgtg cttgttgaga
     2461 atatcaacga atttttaaaa ggaaaaaata actgacttaa aaaggctgtg ctcaaaacac
     2521 agcctttaaa ttacggtaat tatgattaac ttgactcctc gtttgctgaa aacagcagaa
     2581 ttgattactc cgtgcaaaaa gcttgccgac atcggaaccg atcacgcata cataccggtc
     2641 tacgcattac aaaatgatct tgctgaaact gttgtggcct ctgatataaa cgaaggaccg
     2701 ttaaataatg cggcaaaaac cgttaaacaa tacggttttg aagatagagt ggttttacgg
     2761 ctttccgacg gacttgaaaa tattgaagaa accgaagccg acgccgttgt tatagcgggt
     2821 atgggcggtg agcttatagc taaaataatc ggtgacgcac catggcttaa aaataataaa
     2881 cagattatta tgcagccaat gac
//
LOCUS       DBMDVO010000094         2898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15940_length_2898_cov_1.194494, whole genome shotgun sequence.
ACCESSION   DBMDVO010000094 DBMDVO010000000
VERSION     DBMDVO010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15940_length_2898_cov_1.194494"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="ACZPE2_02155"
     CDS             <1..220
                     /locus_tag="ACZPE2_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318682.1"
                     /translation="MRYFYTARAVCASFSAIICMELLKVFPCEVSVFATDSSVTPAAY
                     SVSVPALAALLIMSVLLILEVDSDKKMC"
     gene            255..470
                     /locus_tag="ACZPE2_02160"
     CDS             255..470
                     /locus_tag="ACZPE2_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318683.1"
                     /translation="MKDKMLGGNISPKCEYCEFSTPINDGDTVLCPKKGVVSKDWCCK
                     KYKYDIFKREPKLHPTAPEFSKSDFEI"
     gene            612..2327
                     /locus_tag="ACZPE2_02165"
     CDS             612..2327
                     /locus_tag="ACZPE2_02165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318684.1"
                     /translation="MKKYYALYKKQFILGPAFKLAEAILELLVPLVMAKIIDVGIKNG
                     DVPYIIKMGLLMAGLGILGLLCAIVCQYSAAVAQQGVGTEIRNDMFKKINSFAQSDID
                     AFSSSSLITRITNDVNQIQSGVAMFIRLMFRSPFLIAGSLIMAMKLDLKMSIIFFAAG
                     LLVSLIMYYVLTKSLPLYKVIQKVLDKVSLVVKENLSGVRVIRAFSKSNHEKERFGDK
                     NDELTRINFRVGSLSALLNPMMNLAIVILLYFGGKNVYSGELSQGKIIALISYMTQIL
                     LSIIVFANVIVVFTKALASVQRVKEVLDAEPSIKDDDAAYQNGDEAATAVEFTDVSYA
                     FPKSDENALSNISFTAEKGETIGIIGGTGAGKSTLVNLIPRFSDVKNGSVKVFGHDVR
                     DCKFGYLRHVIHVVPQKSNLFSGTIKDNLLMGDRSADDRDIDFALKVSQSAEFVSKLK
                     DGTDTLIEEKSKNLSGGQKQRLCIARAIMGHPRILIFDDSSSALDFATDAALRKALKE
                     NLNDTTVFLVSQRVNTVKNADKILVLDDGELVATGTHKELYKTNEVYRDICLSQLSEK
                     EVASV"
     gene            2324..2881
                     /locus_tag="ACZPE2_02170"
     CDS             2324..2881
                     /locus_tag="ACZPE2_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1318685.1"
                     /translation="MKNTKRLIKYISPFKSNLFGAIFSAFVGIALSLMVPILIGKGVD
                     CIAAKGDVDFARLFKVGAVLAICIVVSALFQWIMTYNSNKLSFKTVQKMRTEVFEKLT
                     KVPLSYIDSTAHGDIINTVVTDIDIVSTGLLQGFTQIFSGIVTILGTLIFMFTINWYI
                     TLAVVVLTPLSFFVASFIAKHAHKN"
BASE COUNT          862 a          602 c          662 g          772 t
ORIGIN      
        1 aatgcggtat ttttacacgg caagagcggt atgcgcgtct ttttcggcaa taatatgtat
       61 ggagctttta aaggtcttcc catgcgaggt atctgtcttt gcaaccgaca gctctgtaac
      121 tccggcagcc tattcggttt ccgtaccggc tcttgcggca ctgcttataa tgagcgtgct
      181 gctcatctta gaggttgatt cggataaaaa aatgtgttag catagtaata cacaatgtaa
      241 tcggggtgaa acggatgaag gataagatgc ttggcggaaa catttctccg aaatgtgaat
      301 actgtgaatt ttcaacgccg ataaatgacg gcgatacggt gctctgcccc aaaaagggcg
      361 ttgtgtcaaa agattggtgc tgtaaaaaat acaagtatga tatttttaaa cgtgaaccga
      421 aactacaccc gacagcgccc gaattttcaa aaagcgattt tgagatttaa cataacagcc
      481 gtccgagaga caaaagcagt tctcgggcgg ctgttttttc gataaaaaag cgaatatata
      541 cttaagagat tgaaaaagag aataaaagtg atatactatt ataattaaaa tttcattcaa
      601 aaggagcaac tatgaaaaag tattacgctc tttataaaaa gcaatttatc ttagggccgg
      661 cgtttaagct cgccgaggcc atacttgaac ttttggtccc gctcgttatg gctaaaatca
      721 ttgacgtggg aatcaaaaac ggcgatgtgc catacattat aaaaatgggg cttttgatgg
      781 caggcctcgg tatactgggc cttttgtgtg ccatagtatg tcaatacagc gccgccgttg
      841 cccaacaggg agttggcacg gaaatcagaa acgatatgtt taaaaagata aattcctttg
      901 cacaaagcga tattgacgcc ttttcttcgt catcgcttat aacgagaatt acaaacgacg
      961 taaaccagat acagtcgggc gttgcaatgt ttatacgtct tatgtttcgc tcgccgtttt
     1021 taatagccgg ttcgcttatt atggcaatga agcttgatct taaaatgtca ataatcttct
     1081 ttgccgcagg tcttttggtc agtcttatta tgtactatgt tctgactaag agcctgccgc
     1141 tttacaaggt gatacaaaag gtacttgata aggtctcgct tgttgtcaag gaaaatcttt
     1201 caggcgtcag agttatacgc gcattttcaa aaagcaatca cgaaaaagag cgctttggcg
     1261 ataagaatga cgagctgaca agaataaact tccgtgtggg cagtctttcg gcacttctga
     1321 acccaatgat gaacctcgca atagtaattt tgctgtattt cggtggaaaa aacgtatatt
     1381 cgggcgagct gtcgcaggga aaaataatag cacttataag ctatatgaca cagatacttc
     1441 tctcaataat cgtatttgca aatgtcatag tcgtatttac aaaggctctc gcaagcgttc
     1501 agcgcgttaa agaggtattg gacgccgagc cgagcataaa ggatgacgac gcggcatatc
     1561 aaaacggcga tgaagccgct acggcggtcg aattcaccga tgtcagctac gcgttcccaa
     1621 aatccgatga aaacgccctg tcaaacatca gttttacagc cgagaagggc gaaacgatag
     1681 gaattatagg cggtacgggc gcaggcaaaa gcacactcgt aaatctgata ccgcgcttca
     1741 gcgacgttaa aaacggcagc gtcaaggtat tcggccacga tgttcgagac tgtaaattcg
     1801 ggtatttaag gcacgtcata catgttgtac cgcaaaagag caatcttttt tcgggtacaa
     1861 taaaggacaa cctgctgatg ggtgaccgct ccgccgatga cagagacatt gattttgcgc
     1921 tcaaagtttc gcaaagcgcg gaatttgtat caaagctaaa ggacggcaca gacaccttaa
     1981 ttgaggaaaa gagcaaaaat ctgtcgggag gccaaaagca gcgtctttgc atagcaaggg
     2041 caataatggg gcaccctcgg atattgattt ttgacgattc atcaagcgcg cttgacttcg
     2101 ccacggacgc ggctctgaga aaagctttga aagaaaactt aaatgacaca accgtatttt
     2161 tggtatcgca gcgtgtcaac acggtaaaga atgccgataa aatactcgtt ctcgacgacg
     2221 gtgagcttgt ggcaaccggt acgcacaagg agctatacaa aacaaatgaa gtataccgcg
     2281 acatttgtct gtcacagctt tcagaaaagg aggttgcctc cgtatgaaaa acacaaaacg
     2341 tctcataaaa tatataagtc cgtttaaatc caatctcttc ggggcaattt tttccgcatt
     2401 tgtcggcatt gcgctgtcgc ttatggtacc cattctcatc ggtaaaggcg tagactgcat
     2461 agcggctaag ggcgacgtcg atttcgcaag gctttttaag gtcggcgcgg ttctcgcgat
     2521 atgcattgtt gtttcggcgc ttttccaatg gataatgacg tataattcaa ataagctctc
     2581 atttaaaacc gttcaaaaaa tgagaaccga ggtctttgaa aagcttacaa aggtgccgct
     2641 ttcatatatt gactccacgg cacacggcga cataataaat actgttgtca ccgacataga
     2701 tattgtctcc accggtcttt tacagggctt tacccagata ttctccggca ttgtgacaat
     2761 actcggtacg ctgatattta tgttcacgat aaactggtat atcacgctcg cagttgtggt
     2821 gctgactccc ctttcgtttt ttgtcgcttc gtttatagca aagcacgcgc acaaaaatta
     2881 agtaaagcag gcaaaata
//
LOCUS       DBMDVO010000095         2892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_15989_length_2892_cov_1.447868, whole genome shotgun sequence.
ACCESSION   DBMDVO010000095 DBMDVO010000000
VERSION     DBMDVO010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_2892_cov_1.447868"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="ACZPE2_02175"
     CDS             complement(<1..839)
                     /locus_tag="ACZPE2_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1318686.1"
                     /translation="MIDKTIKKVLVIGSGPIIIGQAAEFDYAGTQACRSLKEEGIEVV
                     LVNSNPATIMTDGDIADKVYIEPLNVPTLTKIIEKEKPDSILPTLGGQTGLNLAMELD
                     EKGVLKANGVRLIGIKASSIKKAEDRQEFKDTMEKIGEPCIESLVVHDVEAAVEFSDK
                     IGYPVIVRPAYTLGGTGGGIADDEEELREICSNGLRLSRVNEVLIEKCIAGWKEIEYE
                     VMRDGKGNCITVCNMENLDPVGVHTGDSIVVAPSQTLMDKEYQMLRSAALNIITELGI
                     EGG"
     gene            complement(832..1914)
                     /locus_tag="ACZPE2_02180"
     CDS             complement(832..1914)
                     /locus_tag="ACZPE2_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR1318687.1"
                     /translation="MKAYLMLEDGSVYEGTARGDFKETACEIVFNTSMTGYVEILTDP
                     SYAGQGVVMTYPMIGNYGISREDFESERLQPSAFIVHELSSDPSNFRCTDTIENLLKE
                     YNIPCISGVDTRAVVKKLRESGTMRGVLTSDIGDRERLMDVIKSFRQIDLVGSVTAKQ
                     TKVYGEENTGAEIALMDFGTKRNIINNLVKRNCIVTVYPSFTSADEIIKTSPDGIMLS
                     NGPGDPSECVDIIKEIKKLYDYGIPTFAICLGHQLMALANGGETERMKYGHRGANHPV
                     KFLNEDRTYLSSQNHGYMVNGKGLPDNAEINCINVNDKTVEGIVYHGKPVFTVQFHPE
                     ANAGPRDTAFLFDRFLKMAAEGRYDD"
     gene            complement(2069..>2892)
                     /locus_tag="ACZPE2_02185"
     CDS             complement(2069..>2892)
                     /locus_tag="ACZPE2_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR1318688.1"
                     /translation="PELLVSSIAAAKGQNDMAIGNAVGSVTANIGLIMSISVLCMPAV
                     IKRSSVALKGSLMILAVAALFAFSYDLDLNLWQSIIMIAIFAVFMIENIISGKKSLSA
                     DSGEVEKMEHTGKQMALNIVKFLIGAAAVVVGADLLVDDGTVIARHLGVSEAIIGVTI
                     IAIGTSLPELVTTLTAVAKKQSELSIGNIIGANIIDLTLILPICAFLSGGTLTVGKQS
                     AFLDMPVCLLVIAIAIIPTLIFKKLSRWQGALMVCIYIGYVAVLVTNSSMHYLPF"
BASE COUNT          737 a          743 c          635 g          777 t
ORIGIN      
        1 cagccgccct ctatgccaag ctccgttatg atgttaagag ccgcggagcg cagcatctga
       61 tattccttat ccataagggt ctgagacggt gcgacaacta tcgagtctcc ggtatgaact
      121 cctacggggt caaggttttc catattacat acggtaatgc agttgccctt gccgtcacgc
      181 attacctcat actctatctc tttccagccg gcaatacatt tctcgataag gacctcgttt
      241 acgcgcgaca ggcgaagccc gtttgaacat atctcacgaa gctcctcctc atcgtcggca
      301 attccgccgc ccgtaccgcc gagcgtatat gccgggcgga ctattacggg atagcctatc
      361 ttatcggaaa attcaaccgc agcctcaaca tcgtgtacaa cgagcgactc gatacacggc
      421 tcgccgattt tttccatggt atccttaaat tcctgcctgt cctcagcctt tttgatagag
      481 gacgccttga taccgataag gcgcacgccg ttggccttta aaacgccttt ttcgtcaagc
      541 tccatagcaa ggttgagtcc tgtctgaccg ccgagcgtgg ggagaatcga atcgggtttt
      601 tctttttcta ttatcttggt gagcgtggga acattcagcg gctcgatata caccttatcc
      661 gctatatcgc cgtcggtcat tattgtagcg gggtttgagt ttacaagtac aacctctatc
      721 ccctcttcct tcagagagcg gcacgcctga gttccggcgt agtcaaattc ggcagcctga
      781 cctatgataa taggaccgga gccgataaca agtacctttt ttatcgtctt atcaatcatc
      841 gtatctgccc tccgctgcca tcttcaaaaa tctgtcaaac aaaaacgccg tatcgcgcgg
      901 acctgcgttg gcctccgggt ggaactgcac ggtgaacacg ggctttccgt gataaactat
      961 tccctcaacg gtcttatcgt ttacgtttat gcagttgatt tcggcgttat ccggcagccc
     1021 cttgccgttt accatatagc cgtggttctg cgacgagagg taggtcctgt cttcattaag
     1081 gaatttgacc ggatgatttg cgcccctgtg gccgtacttc attctttcgg tctcgccgcc
     1141 gttggcaagt gccataagct gatggccgag gcagattgca aaggtcggta taccgtaatc
     1201 gtaaagcttc tttatctctt tgattatatc gacgcattcc gacggatcgc cggggccgtt
     1261 tgaaagcata atgccgtcgg gcgatgtctt tattatttcg tctgccgacg taaatgacgg
     1321 ataaaccgtt acaatgcagt ttctcttcac gagattattt attatattcc gctttgttcc
     1381 gaaatccata agtgctattt cagcaccggt gttttcttcg ccgtatacct ttgtctgctt
     1441 tgcggtaacg ctgccgacaa ggtctatttg gcggaaggac ttaatgacat ccataagcct
     1501 ttccctgtcg ccaatatccg aagtgagtac gccgcgcatg gttccgctct cgcgcagttt
     1561 ctttacgacg gcgcgcgtat caacgcccga tatgcacgga atattgtatt cctttaaaag
     1621 attctctatt gtatcggtgc atctgaaatt tgacgggtcg gagctgagct catggactat
     1681 aaaggcactc ggctgaagcc tttcgctctc aaaatcctct cggcttatac cgtaattgcc
     1741 tatcatggga taggtcataa caactccctg ccccgcatac gacgggtcag taaggatttc
     1801 aacatatccg gtcatggaag tgttaaacac tatttcgcag gcagtttctt taaaatcgcc
     1861 ccttgcggtt ccctcatata cggaaccgtc ttcaagcatc aaatacgctt tcaaacaata
     1921 caccgacctt tcaaacaaat gcttttactt aattgaaaat tttatcatat ttcgggttct
     1981 atttcaatac aaatattaaa cataaaaata aaaacgacct ttgaaaatcc cgaaaaaaag
     2041 gagctgtaaa aaatacagcc cctatcattt aaaacggaag ataatgcatg gacgaattcg
     2101 tgacaagcac cgccacatag ccgatataaa tacaaaccat caaagcgccc tgccaacggc
     2161 tgagcttttt gaaaatcaac gtaggtatta tggctatcgc tatgacgagc aggcaaaccg
     2221 gcatatcaag aaatgccgac tgcttgccga ccgtaagagt tccgcccgat aaaaacgcgc
     2281 atatcggaag aatgagagtc aaatcaatga tatttgcgcc tataatgttg cctatggaaa
     2341 gctccgactg ctttttggca acggcggtaa gagttgttac aagctcgggc aaggatgtgc
     2401 ctatcgctat tatggtaacg ccgattatcg cctcggacac gcctaaatgg cgagctatca
     2461 cggtaccgtc atcaacgagc aaatcggctc ccacaacaac ggccgcggca cctatcaaga
     2521 acttaactat attgagtgcc atctgcttcc cggtgtgttc cattttctca acctcgccgg
     2581 aatcggcgga gagagatttt ttgccggaaa tgatgttttc tatcataaag acggcaaata
     2641 tcgctatcat tataatgctc tgccaaagat ttaaatcaag gtcataagaa aatgcaaaaa
     2701 gcgcggcaac cgcgagaatc ataagcgagc cctttaatgc aacggagctg cgtttaatga
     2761 cggccggcat acacaaaacg gaaatgctca ttataaggcc gatattggcg gttaccgagc
     2821 ctacggcatt gcctattgcc atatcgtttt gcccctttgc cgccgctatt gacgaaacaa
     2881 gaagttccgg aa
//
LOCUS       DBMDVO010000096         2891 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16000_length_2891_cov_1.328674, whole genome shotgun sequence.
ACCESSION   DBMDVO010000096 DBMDVO010000000
VERSION     DBMDVO010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16000_length_2891_cov_1.328674"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="ACZPE2_02190"
     CDS             <1..428
                     /locus_tag="ACZPE2_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318689.1"
                     /translation="MELEDYKRFLSLKADNRRKTEYIERIERRNAEAERARAHEEQLS
                     KWNADPEEVVERRCMRESEHSDKTIFCSVNGTAIYVPYGKSVKIKRKYAEVIDRSFTE
                     KERGRERAEAFAREGEQAQKIRGRTALRAVRPFVVYGLF"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <858..2149
                     /locus_tag="ACZPE2_02195"
     CDS             <858..2149
                     /locus_tag="ACZPE2_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318690.1"
                     /translation="AEHTVLSEIQRLAPGEIPDIYPYEAHKNDRLTLAPGDDKIYVLY
                     LQAMIDFSNKEYAAYDNDMALFNESLDTYAKWYVRTHREGEELVSGMYLSAYGIAVSH
                     GYVGTEDDWLVSLKGEKGDTGETPENVVTTDKANVANGYAALDANKNISGANGVVKDD
                     GPLTFIAGGAEKHASGSASVTLPSGQGAIELSAYGVTLCDDQGGSPVWLYTSGGDLYE
                     LKQALTGKNAESIAQMITKLIEEQSSDLSSYITVNGTDVSIKSAAENGINVSTVGVSL
                     TNGYTLASLGQSAPEGSSGNLLTDIGNILSSLTAKQNKPAAVIPESAAGNITLSDNTE
                     YLLNGAAGASTAITLTLPAIDATKGYSSYFVFNSGATPTTITLPASFDGTLKFKGDDC
                     ANGIFTPAANTVYEVALKCVGMDSNNKPYIVARVGAC"
     gene            2152..>2891
                     /locus_tag="ACZPE2_02200"
     CDS             2152..>2891
                     /locus_tag="ACZPE2_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318691.1"
                     /translation="MNRYRSLIGINSVPQYGVRWAGTSSTACERLGDAVGLIANAHKG
                     STQSVRNDFDNIYPWSDIKTCNIDADGNVLAYLGEPSFARDGTNGDVMVEIPKFYYKR
                     VKTGIVEEIWICASKLPGYELHPLFIDNGKEVSKVFHSVYNASSFTDETDNKVKLQSI
                     TGAQPRVRTTRAAFRTYARNKGAVWGIEDISCVNALQLLYLVEYANTNSQSALGSGAD
                     LLAYSANHKALEETTNGNTITISSEYKNV"
BASE COUNT          818 a          695 c          778 g          590 t
ORIGIN      
        1 ggatggagct cgaggattac aagagatttc tgtcgcttaa ggccgataac cggagaaaga
       61 ccgagtacat cgaaagaatc gaacgccgga acgccgaggc agagagggcg agagcgcatg
      121 aggaacagct ctcgaagtgg aatgccgatc ccgaggaggt tgtcgagaga agatgcatga
      181 gagaatcgga gcacagcgac aaaacgattt tctgttcggt gaacggcacg gcgatatacg
      241 ttccgtacgg taagagcgtg aagatcaaga gaaagtacgc cgaggtcatc gacaggtctt
      301 tcaccgaaaa agagagaggc agagaacgcg ccgaggcttt cgcgcgcgag ggagagcagg
      361 cgcaaaaaat cagagggcgg acagctttaa gagctgtccg cccttttgtc gtatacggac
      421 ttttttaggg gactgattga atgtagaatg tgttatggtc ccttggccgg gctgcagtcg
      481 ccccctcaat gccttatacc ggataatttt tatccttttc ggtttgtcgt cctcgaaagg
      541 cgtcagcctt ccgtcgtcct ccgtgccaaa aatgcctaaa aattctctca gtataaggtg
      601 caatgcagtt tttaccgtga agattttttc tgaggcaagc aaacgagcgc agacaatact
      661 gtcgtattat caagtgagtt tgcgcagtat cggggaaaat ccgccgataa aaaccattga
      721 tgggtcggcc gcaacccggc ctaagaaagg agaataaagg agaatatatg aataaaacac
      781 ttaaagaaat aatcgctctg gcagacgagc tgaacccgaa cgccttttca aacgacgtta
      841 aggcaagnnn nnnnnnnagg cggagcacac cgttttatcg gaaattcagc gcctcgcgcc
      901 cggcgagatt cccgacattt acccctacga ggcgcataaa aacgaccggc tgaccctcgc
      961 accgggtgac gacaagatat atgtcttata ccttcaggcg atgattgact tttcaaacaa
     1021 ggaatacgcc gcgtatgaca acgacatggc gctctttaac gagagcctcg acacatacgc
     1081 caagtggtac gtccgaacac acagggaggg cgaggagctc gtctcgggaa tgtacctttc
     1141 ggcatacgga atcgccgtca gccacggcta tgtcggcacg gaggacgact ggctcgtttc
     1201 gcttaagggc gagaagggcg atacgggaga gacgcccgaa aatgttgtta ccacagacaa
     1261 agcgaacgtc gcaaacggct acgccgcact ggacgcaaat aagaacatca gcggcgcaaa
     1321 cggagttgtg aaagacgacg ggcccctcac tttcattgca ggcggagcgg aaaaacacgc
     1381 atcggggagc gcttcggtga ctctcccctc cggccaggga gctatagagc tttcggcgta
     1441 cggcgttacg ctgtgtgacg accagggggg aagcccggtg tggctttaca caagcggagg
     1501 cgacctttac gagctcaagc aggcgcttac cggaaagaac gccgagagca ttgcccaaat
     1561 gataacgaag ctcatcgagg agcagtcgtc cgacttgtca agctatatca ccgtcaacgg
     1621 gactgatgtc agcatcaagt cagcggccga aaacggaatc aatgtcagca cggtgggcgt
     1681 ttcattaacc aacggttaca cgcttgcaag cctcggccag agcgcgcccg aagggtcgag
     1741 cggaaatctt ttgacagaca tcggaaacat actttcctcc ctcactgcga agcagaacaa
     1801 gcctgcggca gtcatcccgg agagcgcggc gggaaacatc acgctttcgg acaataccga
     1861 gtacctctta aacggcgcgg cgggcgcaag tacagcaatc acgctgacgc taccggctat
     1921 cgatgcgact aagggctaca gctcatattt cgttttcaat tccggtgcga ctcccactac
     1981 gataacattg cctgcatcat ttgacggaac actcaagttc aagggcgacg actgcgcaaa
     2041 cgggatattt acaccggccg caaacaccgt gtatgaagtg gccttaaagt gtgtaggcat
     2101 ggatagcaat aataagcctt atatagtagc gagagtaggt gcttgttagt tatgaacaga
     2161 tacagaagcc tgattggaat aaacagcgtg ccccaatatg gggtgcgctg ggcaggcaca
     2221 tcatccacag catgtgaaag acttggagac gctgtcggac ttatagcaaa cgcgcacaaa
     2281 ggaagcactc aaagtgtgag gaatgatttt gataatattt atccatggag cgatattaaa
     2341 acgtgcaata tcgacgctga cggaaacgta cttgcatatc tcggcgagcc gtcctttgca
     2401 agagacggaa cgaacggcga cgttatggtc gaaattccaa aattctatta caagagagtc
     2461 aagacaggca ttgtcgagga aatatggata tgcgcatcaa agctccccgg atatgagctg
     2521 caccctcttt tcattgacaa cggaaaagaa gtcagcaagg tttttcattc ggtatataat
     2581 gcaagctcat tcaccgatga aaccgacaac aaagttaaat tgcagagcat tacgggcgct
     2641 cagccgagag tgagaacgac aagggctgcc ttccgtacat acgcccgaaa caaaggcgct
     2701 gtatggggca ttgaggatat ttcctgtgta aatgcgttgc agttgctcta tttggtggaa
     2761 tatgcaaata caaattcaca gtctgccctc ggttccggcg ctgacctttt ggcttattcg
     2821 gcaaatcata aagcccttga agaaactaca aacggcaaca cgatcactat ttcgtccgaa
     2881 tataagaatg t
//
LOCUS       DBMDVO010000097         2873 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16142_length_2873_cov_1.006133, whole genome shotgun sequence.
ACCESSION   DBMDVO010000097 DBMDVO010000000
VERSION     DBMDVO010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16142_length_2873_cov_1.006133"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(83..910)
                     /locus_tag="ACZPE2_02205"
     CDS             complement(83..910)
                     /locus_tag="ACZPE2_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR1318692.1"
                     /translation="MKKKKDGLIKGMIAAAIIFVVGFILTVCFGAYLGADAVRHIASG
                     ETFEEFVEERFPNIDFDDGQAVNESQTENSMLPSGSALEISAPYGDIKIKASDSTEVR
                     CVLKQANDSGAKLDLSDDKIILDGGGSTYGIAELTVYLPKSVTLLTVSNNDGDIDIDG
                     VNLNMLTINSVSGDIDLSDITAERGKIKSSAGETEISETAVFTKMLEVNQSFGDIEID
                     VPRDPDRIKFIISSETPLDEKTMCDSYLLSLARSTDGKAEYSVNVKNGELSVKNSDD"
     gene            complement(924..1520)
                     /locus_tag="ACZPE2_02210"
     CDS             complement(924..1520)
                     /locus_tag="ACZPE2_02210"
                     /inference="COORDINATES: protein motif:HMM:NF046861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1318693.1"
                     /translation="MTKIEFLQQLYNLLGVLPAEERDDIIADFEEHFSVGTSEGKSEE
                     QICSELGDPKSCAAQYLNDEPKKADAVYGAEKNGNPRYETKASDVIGASPRPQNARGG
                     GYYGTEKGNPQRNHVLWSILFFLGVFAAIGVYPSSIGMMASFFACLFFAGVTGAIATS
                     PAAFGFIFSIGIMLFAAGLFLFLFMTWFLKLSWRRADL"
     gene            complement(1513..1833)
                     /locus_tag="ACZPE2_02215"
     CDS             complement(1513..1833)
                     /locus_tag="ACZPE2_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1318694.1"
                     /translation="MNTQFKKGVLELCVLSLLKRGDKYGYELADTVGKQIDVSEGTIY
                     PLLKRLRAEGYFEVYLRESTSGPARKYYRLTQKGYDTQRLLENEWKDFTKSVSTLLGG
                     ENGD"
     assembly_gap    2010..2019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2180..>2873)
                     /locus_tag="ACZPE2_02220"
     CDS             complement(2180..>2873)
                     /locus_tag="ACZPE2_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR1318695.1"
                     /translation="EDGTNYGDFLISTVEGSKSDFSADEYKILKAAAEQIKEIEGKLT
                     ILEQKHPGCGKKPSSGECVDAASAGMAGDASKLMKFPSFTGKDLDGNDVNSSDLFSGN
                     TVTVVNFWFTSCKPCVSELADLEALNKELAAKGGGVVGINSFTLDGNKKEISEAKDIL
                     SKKGATYKNIWFDTNSEAGKFTSGLYSYPTTYVVDKNGNIVGQPIVGAITSPEQAAKL
                     NKLIDTAIANSK"
BASE COUNT          673 a          751 c          609 g          830 t
ORIGIN      
        1 gaaaatctga cgggtggaac ccctcccgtc agatacctac cctgtgtagt cccttgtaaa
       61 ctgtactttt gtgcgtttta aatcaatcgt cggaattttt taccgataat tcgccgttct
      121 ttacgtttac ggagtattcc gccttgccgt cggtgcttcg tgcaagcgac aaaaggtagc
      181 tgtcgcacat tgttttttca tcaagcggcg tttcggagga aattataaat ttgattctat
      241 cgggatccct cggaacatcg atttctatat cgccgaagct ttggttcacc tcgagcattt
      301 tcgtaaaaac ggcggtttca gatatttccg tctcgcctgc cgagcttttg atttttcccc
      361 tctcggctgt gatgtcggac aaatctatat ctcccgaaac agaatttatc gtaagcatat
      421 tgagatttac tccgtctatg tcgatatcgc cgtcgttatt tgaaaccgtt aagagcgtaa
      481 cgcttttcgg caggtaaacc gtaagctcgg ctattccgta agtactgccg ccgccgtcca
      541 atatgatttt atcgtcggac aaatcgagct tcgcgccact gtcatttgct tgcttcagga
      601 cgcatcttac ctcagtgctg tctgaagcct taattttaat atcgccgtac ggggcggaaa
      661 tttcaagcgc cgaccccgac ggaagcattg aattttctgt ttgcgactcg tttaccgctt
      721 gcccatcgtc aaaatctata ttcgggaagc gctcttcgac aaactcttca aacgtctcgc
      781 ccgatgctat gtgccttacc gcgtccgcgc cgagataagc accgaagcag accgtgagta
      841 taaatccgac gacaaatatt atcgccgcgg ctatcatgcc ctttataagc ccgtctttct
      901 ttttcttcat atttttctcc cccttacaaa tcggccctgc gccatgacag ctttaagaac
      961 cacgtcataa aaaggaatag gaaaagtcct gccgcaaaga gcataatccc tattgaaaat
     1021 ataaatccga atgccgccgg cgaagtcgct attgcgcccg ttactccggc gaagaagaga
     1081 caagcaaaaa agcttgccat catacctatg cttgacggat acactcctat tgccgcgaat
     1141 accccgagga aaaacagaat tgaccacagc acatgatttc gctgcggatt acccttttcc
     1201 gtgccgtaat aaccaccgcc gcgcgcattt tgaggacggg gagaggcacc gattacatcg
     1261 gacgccttgg tttcatatct cggattgccg tttttttctg cgccgtatac ggcgtcggct
     1321 tttttcggct cgtcattaag atattgcgcg gcgcagcttt tggggtcacc gagctccgag
     1381 caaatctgtt cctcgctttt accctcgctc gtgccgacgg agaagtgttc ttcaaagtcg
     1441 gctattatat cgtcgcgctc ctcggccggt aaaacgccga gcaggttgta aagctgttgt
     1501 aaaaattcga tcttagtcac cgttttcacc tcctaataaa gtagacacgc tctttgtaaa
     1561 gtccttccac tcgttttcca aaaggcgttg tgtgtcataa cccttttgag tcagacggta
     1621 atatttccgc gcgggtcccg atgtggattc gcgcaggtac acttcaaaat acccctcggc
     1681 tctgagcctt ttaagaagcg ggtatatagt gccctccgaa acgtctatct gtttaccgac
     1741 ggtgtccgca agctcatagc cgtacttgtc gccacgcttt aaaagcgaca gcacgcaaag
     1801 ctccagaaca ccctttttaa attgcgtatt catcaaactc tcctttccgg cgaagctgaa
     1861 aacgcctgta ttgcagccgt gagccttgcg gcacaaagac gcgctttatg tgcggctgct
     1921 ccgtcctttt aattgcatta taacacccac taccttgcaa tgcaatatac tttgtgatat
     1981 ttaataattt cttaagattt gtgcaaagcn nnnnnnnnna gaacctcctg cggcagtttt
     2041 tgcttctgcc gcaggaggtt tgcgtattgc gaaaagtccg taacaaacat caattgctta
     2101 aaacgaagtg tcggaaacga ataaacggaa aacagcaaac aatatgccga aatacaggat
     2161 tatatatcat ctttgtcaat cacttgctgt ttgcaattgc agtgtcaata agcttgttaa
     2221 gctttgccgc ctgttcgggt gaagttattg caccgactat cggctgaccc actatattgc
     2281 cgtttttatc gacaacatat gtagtcgggt aagaatacag tcccgatgtg aactttcctg
     2341 cctcgctgtt tgtgtcaaac caaatatttt tgtatgttgc gccttttttt gacaaaatat
     2401 cttttgcctc ggaaatctcc tttttattgc cgtcaagagt aaatgaattt atgccgacaa
     2461 ccccgccgcc ctttgcggca agctccttgt tgagtgcctc caaatccgca agctcgctta
     2521 cacaaggttt gcaggaggtg aaccagaagt tgactaccgt tacggtgttg cccgagaaaa
     2581 ggtcgctgct gtttacgtcg ttgccgtcga gatctttacc ggtaaagctc ggaaatttca
     2641 taagcttgct cgcatcgcct gccattccgg cactcgccgc gtcaacgcat tccccgcttg
     2701 acggcttttt tccgcagccg ggatgttttt gctccagaat cgtaagtttt ccttctatct
     2761 ccttaatctg ctccgccgca gccttaagaa ttttatattc atctgcgctg aaatcgctct
     2821 tgctcccctc taccgtgctt ataaggaaat ctccgtagtt tgtgccgtcc tcc
//
LOCUS       DBMDVO010000098         2855 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16280_length_2855_cov_1.364198, whole genome shotgun sequence.
ACCESSION   DBMDVO010000098 DBMDVO010000000
VERSION     DBMDVO010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16280_length_2855_cov_1.364198"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2855
                     /locus_tag="ACZPE2_02225"
     CDS             <1..>2855
                     /locus_tag="ACZPE2_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318696.1"
                     /translation="APGLKVSMQSYKDRAKKIKQSIEDNKAFLTPEGYLAALQNVNSI
                     IDTDTTEDDKTFSDYFNSVAAAGGRDKFNKIVEERAAAQSALKKAGLNENSKYRDIEA
                     ALSDRTIDAGAAPDEMGQGRRYQGRKLTSEDKDYLSSLLLTEDKVSTMTDDEILRKIK
                     DKGNPSYKQKIALGEIDRLTKRNEELIKLAEADGNVSSFNPYGDYAEEYANNQQRMEK
                     LRNMIDNDYAGRDVAYYYTDDNGNSGAMSWENLYYERKVGNAYKAATDKGYGGYNSAA
                     AAAYDEINFIEKVTDAMQWLSQNSTVSAEKKKPYLDIVKEYDEKYGTNYAKEDGIYSI
                     ETKNRINSEIKNSDALNKLKENFKSESGATYDAVKDYAKLQRDKERAAKVQETVQTAV
                     EGVNSVPVVGKALGGIASAISAVPFALVSGTDYIFNLMDSIGHSDYNSDSFRPINTYD
                     DYATNISGEIIGAQTEIYDKAMQEAGVSETARKFWTSTYSGVSSGINSLATVVGSCYA
                     FGPAAGQVVASVILSGQAASSGLQEAVQNGSTTNEALAYGFASGVAEYLGEKISIGSL
                     VDKFLESSFDVTSFKSFMKSFVKDKNLLKMLGQGAVEGSEEVVTEIMNRFSDAVINQD
                     HSKFSNAILTYETEGLSREEAKKKAFLDVLASAAEQAWGGFVGGIMGGTAAVIGGGVS
                     AARNSVADIKNQTDIGKSIAGTDAGKNVLEIAEKRGFDISKASEEVPEGRGVGKLVSK
                     VRNAVRNRNMGKLAQNVVEAELKAAKETAMPIVNNNESLAEMVAEIATGTADVSAISA
                     LSANGVLTEENVEKVFEALEAVQKRTETSEVRNMINDIVETKESSEGKAYNASSIYGI
                     KREGGKTKYILEDGTEVDSGEFGLTSKDKTLFVNAVKGMTDAEANEMISAYRKYANGT
                     DKGVFATTWGLYRSLGETGAAKNGERVAGYKSRIYFT"
BASE COUNT          893 a          633 c          767 g          562 t
ORIGIN      
        1 ggctcccgga ctcaaggtat ccatgcagag ctacaaagac cgtgcaaaaa agataaagca
       61 gagcattgag gacaacaagg cttttctgac tccggagggt tatcttgccg ctctgcagaa
      121 tgtcaactcg attattgata ccgacacgac cgaggacgac aagactttca gcgattattt
      181 caacagtgtg gcagccgccg gcggacggga taaattcaac aaaatcgtcg aggaaagagc
      241 agcggcacag agtgcgctta aaaaagcggg acttaacgaa aacagcaaat acagagatat
      301 cgaagccgct ctttccgaca gaacgatcga tgcgggagca gcccccgatg aaatgggaca
      361 ggggcgcaga taccaggggc gaaagctgac atctgaagat aaagactatc tttcttcact
      421 gctgctgacg gaagacaaag tgtctaccat gacagacgat gaaatactca gaaaaatcaa
      481 agataaaggc aatccgtcat acaaacagaa aatcgcttta ggagagatcg acagactcac
      541 aaagaggaat gaggagttaa tcaaactcgc cgaagccgat gggaatgtgt cttcattcaa
      601 tccttacgga gattacgcgg aagaatacgc aaataaccag cagcgtatgg aaaagctgcg
      661 taacatgata gacaatgatt atgccggacg cgatgtggcg tattactaca ccgacgacaa
      721 cggcaactcc ggcgccatgt catgggaaaa tctgtactat gagcgcaagg tcggtaacgc
      781 atacaaagcg gcgactgata agggatacgg aggatacaac agtgcagccg cagcagcata
      841 cgatgaaatc aattttattg aaaaagtcac ggatgcaatg cagtggcttt cgcaaaacag
      901 cactgtaagt gccgaaaaga aaaagccgta ccttgatatt gtcaaagaat atgacgaaaa
      961 gtacggaaca aactatgcca aagaagacgg aatatacagc attgaaacca agaaccggat
     1021 aaacagcgaa attaagaaca gcgatgcgtt gaacaagctg aaagaaaatt ttaaaagcga
     1081 gtccggggcg acttatgacg ctgttaaaga ctacgcaaag ctgcagcggg acaaggaaag
     1141 agcggcaaaa gttcaggaaa cagttcagac ggcagttgag ggagtgaaca gcgttcccgt
     1201 tgtcggtaag gctctcggcg gaattgcttc ggctatttca gccgtgccgt tcgctcttgt
     1261 ctccggcacc gattatattt tcaaccttat ggattctata gggcacagcg actacaacag
     1321 cgatagtttt cgcccgatta acacctatga tgattatgct acaaacatat ccggcgaaat
     1381 catcggagcc cagacggaaa tatatgacaa agccatgcag gaagccggcg tttccgaaac
     1441 cgcaagaaaa ttctggacaa gcacatacag cggggtttca tccggaatca actcactggc
     1501 tacagttgta ggatcgtgct atgcgttcgg tcccgcggca ggtcaggttg tcgcaagcgt
     1561 tattctctcc gggcaggctg cttcaagcgg tctgcaggag gctgttcaaa acggttcgac
     1621 tacgaacgag gcgctggcat acggttttgc ctccggtgtt gccgaatatc tcggcgagaa
     1681 aatatcaata ggttcgctcg ttgacaaatt tctcgaaagc agctttgacg ttacaagctt
     1741 caagtcgttc atgaagtctt ttgtaaaaga taaaaatctg ctgaaaatgc tgggacaggg
     1801 cgcggttgag ggcagcgaag aagtagttac cgaaatcatg aaccgcttct ctgatgctgt
     1861 cataaatcaa gatcactcaa agttctcgaa cgcaatactc acctatgaga cggaaggact
     1921 cagcagagag gaagccaaaa agaaagcttt tcttgatgtg ctcgcttctg ccgcagagca
     1981 ggcttggggc ggctttgtgg gcggtattat gggcggtact gctgctgtta tcggaggggg
     2041 agtctccgca gcccgcaaca gcgttgcaga cataaaaaat cagaccgata tcggaaagtc
     2101 catcgcggga acggatgcgg ggaaaaatgt gcttgaaatc gctgaaaagc gaggttttga
     2161 catatccaaa gcctcagagg aagtgcccga aggccgcggc gtaggaaagc ttgtttccaa
     2221 ggtcagaaac gcggttcgga acagaaacat gggcaagctt gcacagaatg ttgtcgaagc
     2281 cgagctgaaa gcggcaaagg aaacggcaat gcctattgtg aacaacaatg aaagccttgc
     2341 tgagatggtt gccgaaatcg caaccggcac cgccgatgtc agcgccattt ccgctctttc
     2401 cgcaaacggc gttcttacag aggaaaacgt cgaaaaagtg tttgaggctc ttgaagccgt
     2461 acagaagcgc acagagactt ccgaggtgcg caatatgatc aatgatattg tcgaaaccaa
     2521 ggagtcttcc gaaggaaaag cctacaacgc aagcagcata tacggcatta agcgagaggg
     2581 cggcaaaaca aagtacatac ttgaagacgg cacagaggtt gacagcggcg aattcggtct
     2641 tacatcaaag gacaaaacgc tgtttgtcaa tgccgtaaag ggtatgaccg acgcagaagc
     2701 aaacgagatg ataagcgcgt accgcaagta tgcaaacggc actgacaagg gagtgttcgc
     2761 tacaacgtgg ggactgtacc gctctctcgg cgagacgggg gcggcaaaga acggcgaacg
     2821 tgtagcaggg tataagagca gaatatattt cacgc
//
LOCUS       DBMDVO010000099         2851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16311_length_2851_cov_1.411636, whole genome shotgun sequence.
ACCESSION   DBMDVO010000099 DBMDVO010000000
VERSION     DBMDVO010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16311_length_2851_cov_1.411636"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(106..1503)
                     /gene="uxaC"
                     /locus_tag="ACZPE2_02230"
     CDS             complement(106..1503)
                     /gene="uxaC"
                     /locus_tag="ACZPE2_02230"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR1318697.1"
                     /translation="MKKFMDDDFLLSTDTAKELFRAAKEMPVFDWHCHLSPKEIYDNK
                     NPDDIAELWLSYDHYKWRAMRSFGIDEKYITGDADGYDKFKAWAKTMPYLIGNPLYHW
                     THLELQRYFGIYKTLSPETCDEIWEKCNAKIKKGGFSPRELIEKSGVVCVCTTDDPAD
                     TLEYHKLLAKDKSFSCRVLPAFRPEKALGIELPSFPEWLSRMEKTAGVKIDSYKTLKE
                     VLKERIRYFDSVGCLACDHSFSYIPYEKCGEDELEEIFRKGAAGEKPTEFECDCYKTD
                     LLTELAKEYAAHDWVMELHVGALRNNNTRMFKKIGADSGFDSIDDCNTASGLSKFLDS
                     LDSQNALPKTVIFNLNPRDNFVYGSMIGNFQGSGNYSKIQYGAAWWFNDNYDGMRKQL
                     ATLANLGCLGKFIGMVTDSRSFLSYPRHEYFRRILCEYIGDLVEEGKYPRDMEFLKNI
                     AEDISYNNAAAYFGI"
     gene            complement(1541..>2851)
                     /locus_tag="ACZPE2_02235"
     CDS             complement(1541..>2851)
                     /locus_tag="ACZPE2_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR1318698.1"
                     /translation="DIIPGSSIKEVYEVSHRQYEKIGEAGKRIKNDAETAVAENINTR
                     GGVLVFNPNSFEASGETKLDGKTVYVKNIPPKGYKVVTDVCNTNSVKVTDKFLENRFF
                     KIKLDKTGAFSSLFDKKNKREVLKKGERGNVLTAYEDIPRDYDNWEISNYYTDKSWEV
                     DSVDSIKPLSDGVRAGIEITRRFLSSTIVQRIWLYENTPKIDFENDIDWKESHILLKT
                     AFPTEINADKATYDIQFGTVERPTHKNTSWDAAKFEVCAHKFADLSEYGYGVSLLNDC
                     KYGYDIHDSVIRLTLIKCGNYPNPDADKCRHEFTYSLFPHSGDFREAGTVKLAYLLNS
                     PLEAKKIESQNGTLPEEYSLLSIDSENVICETVKNAEDGDGVIVRLYECCNSRANVNL
                     TLGFDFDDVYLTDLLENEERKLRKHGRTVNLTLKPFEIVTLKLK"
BASE COUNT          674 a          685 c          612 g          880 t
ORIGIN      
        1 ccgttaccct gctgtgtgat gctcggactt tcctcatcgc gcaaagcgct ccgcaactgc
       61 tcgggctgct cactctatgt attttattat attaacctcg gcttgtcaaa tgccaaagta
      121 agccgcggca ttgttatacg atatatcctc cgcgatattt ttaagaaatt ccatatcgcg
      181 cggatatttt ccctcctcca cgagatcgcc tatatattcg cagagaatac gtctgaaata
      241 ctcgtggcgc ggatatgaca aaaagcttct cgaatccgtc accataccga taaatttgcc
      301 gagacatccg agatttgcga gcgtcgcaag ctgctttctc attccgtcgt aattgtcgtt
      361 gaaccaccac gccgctccgt actgtatttt tgaatagttg ccgctgccct ggaaattgcc
      421 tatcatagag ccgtagacaa aattgtcacg cgggttaaga ttaaaaatga ccgttttggg
      481 gagagcgttt tggctgtcaa gcgagtcgag gaattttgaa agccccgaag ccgtgttgca
      541 atcgtctatt gaatcaaagc ccgaatcggc accgattttc ttaaacattc ttgtgttgtt
      601 gttgcgaaga gcacctacgt gcaactccat gacccaatcg tgtgccgcgt attcttttgc
      661 aagctcggtt aaaaggtcgg ttttgtagca gtcgcactca aattctgtcg gtttttcgcc
      721 tgccgcaccc tttctgaata tctcttcgag ctcatcctcg ccgcattttt catatggtat
      781 atatgaaaaa gaatgatcac aggcaaggca gcctacggaa tcgaaataac gtattctttc
      841 tttaaggact tcttttaacg tcttataaga atctattttc acgcccgccg ttttttccat
      901 acgtgagagc cactccggaa acgacggcaa ttctataccg agagccttct ccggtctgaa
      961 cgccggaagc acgcggcatg aaaagctctt atccttagcc aaaagcttat ggtattcaag
     1021 tgtatcggcc gggtcgtctg ttgtgcagac gcacacaaca cccgattttt ctataagctc
     1081 gcgcggagaa aagccgcctt ttttgatttt tgcgttgcat ttttcccata tctcatcgca
     1141 cgtttcggga gacagcgttt tataaattcc gaaatatctt tgaagctcca gatgagtcca
     1201 atgataaagc ggattaccta taagataggg cattgtttta gcccacgcct taaacttatc
     1261 atagccgtcg gcgtcgcccg tgatatattt ttcgtcaatg ccgaacgaac gcatggcgcg
     1321 ccacttgtag tggtcataag aaagccacag ttcagctata tcgtcgggat ttttgttgtc
     1381 gtatatctcc ttcggtgaca ggtggcaatg ccaatcgaat acaggcattt cttttgccgc
     1441 cctgaagagc tcctttgcgg tatcggttga cagcaaaaag tcgtcgtcca taaatttttt
     1501 cattttaagc acctatcctt tcgaaaaaaa gcaatctgtt ttatttcagc tttaaagtta
     1561 ctatttcaaa cggtttcaac gtaagattta ccgttctgcc gtgttttcga agctttctct
     1621 cttcattttc gagaaggtcg gtcaagtaca catcgtcgaa atcaaagccg agcgtgagat
     1681 ttacatttgc gcgtgaatta cagcactcgt acaatctcac gattacgccg tcgccgtcct
     1741 ctgcattttt cactgtctcg catatgacat tttccgaatc aatcgacaaa agggaatatt
     1801 cttcgggaag agtaccgttt tgactttcga tttttttcgc ctcaagcgga gaattgagca
     1861 gataagcgag ctttaccgta cctgcctccc tgaaatcacc gctgtgaggg aacagcgaat
     1921 acgtaaactc gtgcctgcat ttatcggcgt cgggattggg atagtttccg cacttaatca
     1981 gcgtaaggcg aataaccgag tcgtgaatat catagccgta cttgcagtcg tttaaaaggc
     2041 ttacgccgta gccgtattcg gaaaggtcgg cgaatttatg cgcgcacacc tcaaattttg
     2101 ccgcgtccca gcttgtgttt ttgtgagtcg gtctttcaac cgtaccgaat tgtatgtcat
     2161 atgtcgcctt gtcggcattt atttccgtgg ggaaggcggt tttgagaagt atatggcttt
     2221 ctttccagtc aatatcgttt tcgaagtcga ttttcggcgt gttttcatag agccatatgc
     2281 gctgtactat ggtgcttgag agaaatcttc ttgttatctc gatgccggct ctgacaccgt
     2341 ccgacagcgg ttttattgaa tctacggaat caacttccca agacttgtcg gtatagtaat
     2401 tgcttatctc ccagttgtcg tagtcacgcg gaatatcttc gtatgcagta agcacatttc
     2461 cgcgctcacc ctttttgagc acctcgcgtt tatttttctt atcgaacaga gaagaaaacg
     2521 ctccggtctt atcaagcttt attttaaaga acctgttttc aaggaattta tcggtaacct
     2581 ttacggaatt tgtattgcag acatccgtta caaccttata tcccttcgga ggtatatttt
     2641 taacgtacac ggttttgccg tcaagctttg tttcgccgct cgcctcaaag gagttcggat
     2701 taaaaacgag cactccgccg cgtgtgttta tattttccgc gacggcggtt tcggcgtcgt
     2761 ttttgattct tttgcccgct tcgccgattt tctcatactg ccgatgcgat acctcatata
     2821 cttcctttat tgatgagccg ggtattatat c
//
LOCUS       DBMDVO010000100         2784 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16885_length_2784_cov_1.395080, whole genome shotgun sequence.
ACCESSION   DBMDVO010000100 DBMDVO010000000
VERSION     DBMDVO010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16885_length_2784_cov_1.395080"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="ACZPE2_02240"
     CDS             complement(<1..92)
                     /locus_tag="ACZPE2_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HIR1318699.1"
                     /translation="MLDKNKVKLGIAPIAWTNDDMPDLGSENTF"
     gene            complement(94..2010)
                     /gene="iolD"
                     /locus_tag="ACZPE2_02245"
     CDS             complement(94..2010)
                     /gene="iolD"
                     /locus_tag="ACZPE2_02245"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HIR1318700.1"
                     /translation="MAYMTTAQALVKFLDNQYVSFDGKETKFVDGIFTIFGHGIVCGL
                     GEALDSDRGGLTVYQGKNEQGMAHVATAFAKQNNRRKIIACSSSIGPGAANMITAAAT
                     ATVNHIPLLLFPADSFASRQPDPVLQQFEQVNSLATTTNDAYKAVTRYWDRISRPEQL
                     MSAMINAMRVLTDTAETGAVCISLPQDVEGESYDFPDEFLAKRVHKITRIVPSLDEVN
                     DIAEILLNAEKPLVICGGGVRYSEAGEALEMFCKSFGIPFAETQSGKTACLSSDPLNL
                     GGLGVTGNSAGNDIAKCADVILGIGTRFSDFTTGSKSLFRSDAKFITINTSRFDAYKL
                     GAVKLVADAKLGIIALGKALSSHEYRTKYTDEIAKAKKCWNDEMERFAKYSYNESFTT
                     LIKAQYERSVPEFAEKFGTITQVAAVEKIREIIPHDAICVGSAGSLPGDLQRMWTSDS
                     RYSYNMEYGYSCMGYEIAGALGSKLAEPQKEVYAMCGDGSYLMLHSELITSIQEHKKI
                     NVLLFDNSGFGCINNLEMSNGIGNLATEFRFRDENGDLLGDLVPIDFAKSAEGYGVKT
                     YTARTLEQLENALRDSLDSKVSTLIDIKVLPKTMTDGYGAWWNVGVASTSDNKAVNEA
                     YKNKKENESKARKY"
     gene            complement(2023..>2784)
                     /locus_tag="ACZPE2_02250"
     CDS             complement(2023..>2784)
                     /locus_tag="ACZPE2_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR1318701.1"
                     /translation="NPKFNQNNEKILCERNGKNSDMLMDIRVKILKAGEKMTFCEAEN
                     ETALLLMAGDVDFVFDGKTENAKRRSPFLDKATAIHFARNNTVTVKANEESRIVIEQT
                     DNSNDFGVHYYKGEDMTVQHFGADQWDGTADRQVLTVFDYANAPYSNLVLGEVFHKPG
                     CWTSYPPHYHPQPEVYYYEFDKPQGFGVGFNGDDCYKVGDKGCLCITPMHTHQQAAAP
                     GYKMCYVWMIRHLDGDPWRKTRIDAPEHKWLLDDK"
BASE COUNT          624 a          686 c          644 g          830 t
ORIGIN      
        1 tcaaaggtgt tttccgagcc taaatcgggc atatcgtcat ttgtccaagc gataggtgca
       61 atacctaatt taactttgtt cttatcaagc atatcaatat ttcctcgctt tactttcgtt
      121 ttctttttta tttttgtaag cctcgttgac cgccttgttg tcggaggttg acgccacgcc
      181 gacattccac catgcgccat agccgtcggt catggttttc ggcaaaacct taatgtctat
      241 aagcgtcgaa accttagaat caaggctgtc acgaagtgca ttttcaagct gctcaagagt
      301 tcttgccgtg tacgttttaa ctccgtagcc ttccgcgctt tttgcaaaat cgataggaac
      361 aaggtcgccg agcagatctc cgttttcgtc acggaagcgg aattccgttg cgagattgcc
      421 tatcccgttt gacatttcaa gattgtttat acagccgaag ccgctgttgt caaacagcag
      481 tacgtttatt tttttatgct cctggattga tgtgataagc tctgagtgca gcataagata
      541 gctgccgtcg ccgcacatcg catacacctc tttttgcggc tctgcaagct ttgaacccaa
      601 cgcgcccgcg atttcatagc ccatacagga gtagccgtat tccatgttgt aggaatatct
      661 gctgtccgag gtccacatcc tttggagatc tcccggcaga gaacctgcgg agcctacgca
      721 aatcgcgtcg tggggaatga tctcgcgtat tttttcgact gccgccacct gcgttattgt
      781 gccgaacttc tctgcaaact ccggaacgga gcgttcgtac tgagctttta taagtgtggt
      841 gaagctttcg ttgtagctgt attttgcaaa gcgttccatc tcatcgttcc agcacttttt
      901 tgcctttgcg atttcatcgg tgtattttgt tctgtattcg tgagacgaca aagccttgcc
      961 gagagcgatt attccgagct ttgcgtccgc gacgagcttt acggcaccta atttgtacgc
     1021 gtcaaagcgg gaagtgttaa tggttataaa ttttgcgtcg cttctgaaaa ggctctttga
     1081 gccggtggta aagtcggaaa aacgagtgcc tatgccgaga atgacgtcgg cacattttgc
     1141 aatgtcgttt cctgccgaat tacccgtgac tccgagtccg ccgaggttca gtgggtcgct
     1201 tgagagacag gcggttttgc cgctctgtgt ttcggcaaat ggaatgccga atgatttgca
     1261 gaacatctca agtgcttctc ccgcctcgga atatcttact ccgccgccgc agataacaag
     1321 cggcttttcg gcgtttaaaa gaatttcggc tatatcgtta acctcgtcga gagaggggac
     1381 gattcttgta attttatgta ctctctttgc gagaaattcg tcggggaaat catagctttc
     1441 accctcaacg tcctgcggaa gagaaatgca cacggcgccc gtttctgccg tgtcggttaa
     1501 aacgcgcatt gcgtttatca tggccgacat caactgctcc ggacgtgaaa ttctgtccca
     1561 atatcttgtg accgccttgt aagcgtcatt tgtcgttgtg gcaagcgaat ttacctgctc
     1621 aaattgctga agcacggggt ccggctggcg tgacgcaaag gaatctgcgg gaaacagcaa
     1681 cagcggaatg tggtttaccg tagccgttgc ggcggcagtt atcatatttg cggcaccggg
     1741 accaatggaa gaagaacagg ctattatttt tctgcgatta ttctgttttg caaatgcggt
     1801 ggcaacgtgt gccatgccct gctcgttttt gccttgataa accgtgagtc ctcctctgtc
     1861 ggagtcaagt gcttcgccga gcccgcaaac tattccgtga ccgaatatcg taaaaattcc
     1921 gtcaacgaat tttgtctcct tgccgtcaaa cgatacgtat tgattgtcaa ggaattttac
     1981 gagagcctgc gcggtagtca tatatgccat agttttatct ccttatttat cgtctaaaag
     2041 ccatttgtgc tccggggcgt ctattcttgt ctttctccac gggtcgccgt caagatgcct
     2101 tatcatccaa acatagcaca ttttatatcc tggagccgct gcctgctgat gtgtatgcat
     2161 aggtgttatg caaagacagc ccttgtcacc caccttatag caatcgtctc cgttaaagcc
     2221 gacgccgaag ccctgcggct tatcaaattc gtagtagtag acctcgggct gcggatgata
     2281 atgcggcgga tagctcgtcc aacagccggg cttgtggaaa acctcgccca agacaagatt
     2341 tgaatacgga gcatttgcat agtcaaacac cgtaagcacc tgtctgtcgg ccgtgccgtc
     2401 ccattggtct gcgccgaagt gctgaactgt catatcctcg cctttataat aatgaacgcc
     2461 gaaatcgttt gagttatcag tttgctcaat tacaattctc gactcctcgt tcgccttaac
     2521 cgttaccgtg ttattccttg caaaatgaat tgccgttgcc ttgtcaagaa acggagaacg
     2581 tctctttgca ttttcggttt ttccgtcaaa aacaaaatcg acatctcccg ccatcaaaag
     2641 cagagctgtt tcattttccg cttcgcaaaa ggtcattttt tcgcccgctt tgagaatttt
     2701 aactcttata tccatcagca tatcggagtt tttgccgttt ctctcgcaga ggattttttc
     2761 attgttttgg ttaaatttag gatt
//
LOCUS       DBMDVO010000101         2780 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_16919_length_2780_cov_1.139604, whole genome shotgun sequence.
ACCESSION   DBMDVO010000101 DBMDVO010000000
VERSION     DBMDVO010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16919_length_2780_cov_1.139604"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="ACZPE2_02255"
     CDS             <1..974
                     /locus_tag="ACZPE2_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000220 - vacuolar proton-transporting
                     V-type ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V0D/AC39 family V-type ATPase subunit"
                     /protein_id="HIR1318702.1"
                     /translation="VDLANCRTINEAANYLKGRTSYSEAFVSVPNVKIHRARLEAVLK
                     RYMLSRIASLCSFEKAIGQNLYEILLLRNDVDCIITCADYLDSDNIGEYLLFVPDFFK
                     EHSELTMLPLERARNFDELLSGLHGTRYESIIKKAMNGKTEFSVQLLENVLYNYLYTE
                     ASSIICEKYKKGKKRDELLDVFRMRSDMKTIESIYRLKKYYGSGSDIHTGSFFNSGIT
                     SFSEKELASLLAASSPDEVLELLKKTRYGKYLPAGDMVIERKTAIMQLRINEKQLRYS
                     THPETVFLSYVGIMENEMQNVTHIIEGIRYSLPPEEIMKYLIKSDDK"
     gene            974..1909
                     /locus_tag="ACZPE2_02260"
     CDS             974..1909
                     /locus_tag="ACZPE2_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I domain-containing
                     protein"
                     /protein_id="HIR1318703.1"
                     /translation="MKKEKFKRVRVLCSEDSLRDVLAALLSGNFKSEVPAAKNEAALD
                     FRRVEGDDPYEKPIKRLSSVAKYAGWKYTLPEKKQDLPQDFDSKTEEIYNKYKKFKRD
                     KQLLLEQREECEEGKKRIRLFSGLPVDIQALTECEFVKARFGHMPKDCLKRLLEIHKD
                     DPYIEFFKSYESETDCWGIYLVPRSDFERVDSVFASMLFEPINIPSASGEAEKVEEQI
                     DENIEILNRQILEAEALEEYYFKLHFEFCNSLYHTLVNESEKFEILKSGLLNNNSYIL
                     CGLLPLSTKDETEQRLKEIDGAFYTESETVKIKLS"
     gene            1947..>2780
                     /locus_tag="ACZPE2_02265"
     CDS             1947..>2780
                     /locus_tag="ACZPE2_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1318704.1"
                     /translation="MAIEKMKLVKISGSLTKLYDLSARLCESECFHPDSAAKYISSSM
                     GFVPFVDDNPYAGELSELRDIAKAAKFDLEFKSIEESDSDVDEKDASYLKDVEKKVIG
                     LYEQRSSLLDQKAVCEGGIEKYSHFKGLGIDLDQVSQCEFVKIRFGHMPKESYEKLKT
                     VFKDNPYVMFYPCSEDKTDYWGAYFAPSDKVNEIDGIFAFLLFEPFEVPGAAGTVEEV
                     IEQIKKSIEIIKSQIKETDDKIRELWQTEHDHCNKIYSNLVYQNSLYELRSYALHNDK
                     YF"
BASE COUNT          834 a          550 c          680 g          716 t
ORIGIN      
        1 atgttgatct tgcaaactgc cgcaccatca acgaggctgc caactattta aagggcagga
       61 cttcatacag cgaagcgttt gtttcggtac ctaatgttaa aatacatcgc gctcgtcttg
      121 aggctgtttt aaagcgctat atgctttcgc gtatagcgtc gctttgttct tttgaaaagg
      181 caataggtca aaatctctat gagatattac tcctcagaaa cgacgttgac tgcataataa
      241 cctgcgccga ttatcttgac agcgacaata taggcgaata tcttctgttt gttccggatt
      301 tctttaagga acattcggag ctcaccatgt tgcccttaga aagagcacgt aattttgacg
      361 agctgctgtc ggggcttcac ggtacaagat atgagagcat aatcaaaaag gcaatgaacg
      421 gcaaaaccga attttccgtt cagcttcttg aaaatgtgct ttataattat ttgtataccg
      481 aagcttcgtc gataatatgc gaaaaatata aaaaaggcaa aaagcgcgat gagctgctcg
      541 acgtcttccg tatgagatcc gatatgaaga ccatcgagag tatttacagg ctcaaaaaat
      601 attacggcag cggttcggat atacatacag gaagcttctt taattccgga atcacctcct
      661 ttagcgaaaa ggagcttgcg agtcttttgg cggcatcgtc tccggacgag gttttggaat
      721 tacttaaaaa gacgcgatac ggcaaatatc ttcctgcggg cgatatggtg attgaacgca
      781 agactgcaat aatgcagctt cgcataaacg aaaagcaact tcgatattcc actcatcccg
      841 aaacggtatt tttgagctat gtcggaataa tggaaaacga aatgcaaaat gtaacccata
      901 tcatagaggg catcaggtac tcgcttccgc ccgaggaaat aatgaaatat ctgataaagt
      961 cggatgataa gtgatgaaaa aagagaaatt taaaagagtc cgtgtgcttt gcagcgaaga
     1021 cagcctccgc gatgttttgg cggcgctgct gtcgggtaat ttcaaaagcg aggtgccggc
     1081 agccaaaaac gaggccgcgc ttgatttcag gcgcgtcgag ggcgacgacc cttatgaaaa
     1141 acccataaaa cggctctctt cggtggctaa atatgccggt tggaaatata ctttgccgga
     1201 aaagaaacag gatttgccgc aggactttga ctctaaaacc gaagagatat acaataaata
     1261 caagaagttt aaacgcgata agcagctgct ccttgagcaa agagaggagt gcgaggaggg
     1321 caaaaaacga attcgcctgt tcagcggcct ccctgtggat attcaagctc ttaccgagtg
     1381 cgaattcgtc aaggcgcgct tcggccatat gccgaaggat tgtcttaaaa ggcttcttga
     1441 aatccataag gatgacccgt acatagagtt tttcaaatcg tatgagagcg agacggattg
     1501 ctggggcatt tatcttgtgc cgcgttcgga ttttgagcga gtagattcgg tttttgcgtc
     1561 gatgctgttt gaaccgataa atattccgtc ggcatccggc gaagccgaaa aggttgagga
     1621 gcagatagat gaaaacatcg aaattctcaa ccgacagata cttgaagccg aggctcttga
     1681 ggaatattat tttaagctgc atttcgagtt ttgcaattcg ctttatcaca cgcttgtgaa
     1741 cgagtccgaa aaatttgaaa tattaaagag cgggctttta aacaataatt cgtatatcct
     1801 gtgcggtctg cttccgctct cgacaaagga cgagacggag caaaggctca aagagataga
     1861 cggtgcattt tatacagaat cggaaacagt caagattaag ttgtcctgac tgaccttata
     1921 aaaaccgaaa ggagacggta ggggctatgg ctatcgaaaa gatgaagctt gtcaaaataa
     1981 gcggcagcct tacaaagctt tatgacttga gtgcaaggct ttgcgagagc gagtgttttc
     2041 atccggattc cgcggcgaaa tacatatctt cgtcaatggg attcgtaccg tttgttgatg
     2101 acaatccgta tgccggtgag ctgtcggagc ttcgtgatat agcgaaagct gcaaaatttg
     2161 atctcgaatt taaaagcata gaagaaagcg attcggacgt tgacgaaaag gacgcgtctt
     2221 atcttaagga cgttgaaaaa aaggttatag gactttatga gcagcgaagc tcgttgctcg
     2281 accaaaaggc ggtatgcgag ggcggtattg aaaaatattc gcactttaag ggccttggta
     2341 ttgaccttga ccaggtatcg caatgcgaat tcgtcaaaat acgtttcggt catatgccga
     2401 aggagagcta cgaaaagctg aagacggtgt tcaaagataa tccgtacgtt atgttttatc
     2461 cttgctccga ggataaaacg gattattggg gcgcatattt tgcaccgagt gacaaggtga
     2521 acgagattga cggtatattt gctttcctct tgtttgaacc gtttgaggtt cccggagcgg
     2581 caggcaccgt tgaggaagta atcgagcaga ttaaaaagag cattgagata atcaagtctc
     2641 agataaaaga gacggacgat aaaatccgag agctttggca gaccgagcac gaccattgca
     2701 acaagattta ttcaaatctt gtatatcaga attcgcttta tgagctccgc tcttacgcgc
     2761 tgcacaatga taagtacttt
//
LOCUS       DBMDVO010000102         2763 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17083_length_2763_cov_1.241548, whole genome shotgun sequence.
ACCESSION   DBMDVO010000102 DBMDVO010000000
VERSION     DBMDVO010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17083_length_2763_cov_1.241548"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="ACZPE2_02270"
     CDS             <1..810
                     /locus_tag="ACZPE2_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318705.1"
                     /translation="IQKEQAEQKSKRNTDSSSSSGSSSSGSASSSASSDNTASASSDN
                     SSAAQGNAAAPTELSASSSVEDVVNYFNKAINNVKPNAKEITLTKETNSQAGSISGDL
                     PKSLTSLADSLIAANMGDKDVSTVSPATSVEDKNAMFPVENESWSSKLTADDVESKEV
                     VDNGSSYVITLYIKADEPSTDTAHGNGHNGKVFSVVMPSIVTDNAGGAAKIIKDVKTG
                     HSDGYVKITVDKASGNVTEATYYFVWTLSLTALSANVSIPFGLEKNFTIAW"
     gene            complement(1033..>1467)
                     /locus_tag="ACZPE2_02275"
     CDS             complement(1033..>1467)
                     /locus_tag="ACZPE2_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1318706.1"
                     /translation="LLNDAVKKGGVMASSHVGGLSGAFIPVSEDAGMIDAARSGTLKI
                     EKLEAMTAVCSVGLDMIAVPGDTPAEVISAIIADEAAIGMVNSKTTAVRVIPAIGRSD
                     DDEVEFGGLFGSSPIMKVNTSSPAKFINRGGRIPAPLQSLKN"
     assembly_gap    1468..1477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1478..2357)
                     /locus_tag="ACZPE2_02280"
     CDS             complement(<1478..2357)
                     /locus_tag="ACZPE2_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1318707.1"
                     /translation="MINTQDILETINMIREENLDIRTITMGISLLDCADSDADKACGR
                     IYDKICRKAENLVKTGEEIEKEYGVPIINKRISVTPVSMIFASAGGDPIKYAKTLDKA
                     AKAVGVNFLGGYSALVQKGFAKGDEELIRSIPEALAVTERLCSSVNIGSTKAGINMDA
                     VALMGKIVRESAVLTKDKGLVGPSKLVVFCNAPDDNPFMAGAFHGAGEPDSVINVGVS
                     GPGVVRSALSKIPDGNITDVADVVKKTAFKITRVGQLVASRAAEKLGVPFGIVDLSLA
                     PTPAVGDSVAHILEELG"
     gene            complement(2390..2659)
                     /locus_tag="ACZPE2_02285"
     CDS             complement(2390..2659)
                     /locus_tag="ACZPE2_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1318708.1"
                     /translation="MRSIITVVGKDTVGILAEVSTLCAKHSVNIIEVTQSILQDMFCM
                     IMLVDVDKCDIPFTSFADEISSLGEKTGLSMNAVHEDIFNTMHHI"
BASE COUNT          735 a          644 c          606 g          768 t
ORIGIN      
        1 atccagaagg agcaggcaga gcagaagagt aagagaaata cggacagcag ctcttcatcg
       61 ggcagcagct cttcaggcag tgcttcaagc agtgcttctt ccgataatac cgcttctgct
      121 tcttcggaca actcttcggc tgctcagggc aatgccgcag ctcccacaga gctttccgca
      181 tcaagttcgg tagaagatgt tgttaattac ttcaataagg ccatcaataa tgttaagccg
      241 aatgctaaag agataactct tacaaaagag acaaactccc aggcaggcag catttcaggt
      301 gatcttccca agagtcttac aagtttagct gattccctta tagctgcaaa tatgggcgac
      361 aaggatgttt ctacagtttc tccggcaact tccgttgaag ataaaaacgc tatgttcccc
      421 gtagagaacg agagctggtc aagtaagctt acagctgacg acgtcgagag caaggaagta
      481 gttgataacg gcagctctta tgttataact ctttacatca aggcagatga gccgtcgacg
      541 gacaccgctc acggcaacgg ccacaacggc aaagtattct ctgttgttat gccgtcaata
      601 gttaccgata atgcaggcgg tgcggcaaag attatcaagg atgtaaaaac aggtcacagc
      661 gacggttatg ttaagattac cgttgataag gcgtcgggca atgttactga ggctacatac
      721 tatttcgtat ggactctttc tcttaccgca ttgtcagcga atgtttcaat tcccttcgga
      781 cttgaaaaga atttcacaat tgcttggtaa ttaagctaaa caaacaaatc ttaaggcacc
      841 tcatttgagg tgccttttat ttttgctgtt gtaccgatag taaaaaccga tttcttgcgg
      901 ataaagctat cggtatcttt tcagcattgc aaaatgtaaa cggcagcaat aacagcagtt
      961 gcaaaaaaca acagtaaaaa aagaggtaca ccgaaaacag cgtacctctt tttgcctttg
     1021 taattgtaag ccttagtttt tgaggctctg aagcggagcc ggtattcttc cgccgcggtt
     1081 aatgaactta gcgggggaag aggtgttaac tttcattatg ggcgaactgc cgaaaagacc
     1141 gccgaattca acctcatcgt catcgcttct gccgattgcg gggataactc ttacggctgt
     1201 ggtctttgaa ttaaccatac cgatagccgc ctcatcggcg attattgcgg atattacctc
     1261 ggcaggtgta tcacccggta cggctatcat atcaagtcct acggagcata ctgcggtcat
     1321 tgcctcaagc ttttctattt tgagcgttcc gctccttgcg gcgtctatca ttcccgcatc
     1381 ctcggaaacg ggtataaatg cgccggaaag tcctccgacg tgtgaggacg ccataacgcc
     1441 gccttttttt actgcgtcat tcaaaagnnn nnnnnnngac ccaattcttc aagaatgtgc
     1501 gcaaccgaat ctccgaccgc cggtgtgggg gcaagagaca agtctacaat gccgaacggg
     1561 acaccgagct tttctgcggc tcttgatgct acaagctgac cgactctcgt tattttgaat
     1621 gcagtctttt taacaacgtc ggcaacgtcc gtaatatttc cgtcgggtat ttttgagaga
     1681 gcagagcgaa caactccggg tccggatacg cctacgttga taacgctgtc gggctctccc
     1741 gcaccgtgaa aagcacctgc cataaacggg ttgtcatcgg gagcgttgca aaacacaacg
     1801 agctttgacg gtcctacaag acccttatcc ttagtaagaa cagccgattc gcggactatt
     1861 tttcccataa gggcaacagc atccatattt ataccggctt ttgtggaacc tatgttaacg
     1921 cttgagcaga gtctttcggt tacggcaaga gcctcgggta tgcttcttat aagctcctcg
     1981 tctccctttg caaacccctt ttgcacaagt gccgaatatc cgcctaaaaa gtttactccg
     2041 actgcctttg cggccttgtc aagagtcttg gcgtatttta tggggtcgcc tccggcagac
     2101 gcgaatatca ttgacacggg agttaccgat attcgcttgt tgattatcgg aacaccgtat
     2161 tccttttcta tctcttcacc tgttttgacg aggttttcgg ccttgcggca tattttatcg
     2221 taaattctgc cgcacgcctt gtcagcgtcc gagtccgcac agtcaagaag agatattccc
     2281 atagtgatgg ttcgtatgtc gagattttcc tcccgaatca tattgatagt ttcaagtata
     2341 tcctgtgtgt ttatcataga attcttccct tacttggaat atttataaat tatatgtgat
     2401 gcatcgtatt aaaaatgtcc tcgtgaacgg cattcatcga aagtccggtc ttttcaccga
     2461 gagaggagat ttcatccgca aaagaggtaa atggtatatc gcatttgtca acgtcaacga
     2521 gcattatcat acaaaacata tcctgtaaaa tcgattgtgt gacctcaatt atgttgacgg
     2581 aatgcttcgc gcagagcgta gatacttcgg cgagtatgcc cacggtgtcc ttaccgacaa
     2641 cggttataat agaacgcata aaatgtacct ccaaaaaaat tcttaggata ttttatcaca
     2701 cttttgttcc gtttaacata tcaaatgaca atttttttca aaaaacagta tattcttttt
     2761 tta
//
LOCUS       DBMDVO010000103         2751 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17183_length_2751_cov_1.024906, whole genome shotgun sequence.
ACCESSION   DBMDVO010000103 DBMDVO010000000
VERSION     DBMDVO010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17183_length_2751_cov_1.024906"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(36..111)
                     /locus_tag="ACZPE2_02290"
     tRNA            complement(36..111)
                     /locus_tag="ACZPE2_02290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Lys,seq:ttt)
     gene            complement(142..390)
                     /gene="secG"
                     /locus_tag="ACZPE2_02295"
     CDS             complement(142..390)
                     /gene="secG"
                     /locus_tag="ACZPE2_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1318709.1"
                     /translation="MSALEIILAVLLILSSVILTVVILMQESRQAGLSGAIAGGADTF
                     FGKNKGRTMEAKLSRFTKYFGLFFFVLALGATLLLLFF"
     gene            complement(656..1123)
                     /locus_tag="ACZPE2_02300"
     CDS             complement(656..1123)
                     /locus_tag="ACZPE2_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318710.1"
                     /translation="MLETDTEKNSSYGSKHALPRAGEQKKRACEEFQYDFSNGTKRSE
                     ARPEETYFYGENQPMMFEETVKAKNGGGSKNAEKGLAVFLTVAAVLLALVSAFSILTG
                     FGIIKNYTGLFEKTTTEAAADTDKGSNYIYNSGISAETSESETSSGESITTGQ"
     gene            1347..>2751
                     /locus_tag="ACZPE2_02305"
     CDS             1347..>2751
                     /locus_tag="ACZPE2_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318711.1"
                     /translation="MGENATSTTVAEPSEQELEKEVKKASEQNRRYIRPSELVAFLLT
                     TFGQKNLDQFVNAYRQFFMISFLGISGRAYGLIVFIESIYDAVDDSLSGLIIDRTRTR
                     WGRLRPYMIITVPIWGLATLMLFTTPQFLSGNTSKIVWAVIAIFAYNLGMSYFNAWQI
                     LLYNITPSLRERDNLIATSKFFELFGTWIPSLVPLFVSFMPKISESIGMQDVYSGFAV
                     AMMVIAGGTAIYGFFAMRERVPLASREQMEEISVLDSFKNAIKNKPMFILMLANFFNG
                     FKSVGASTESFFWLHNTGSLANGTIAGVFTGIPNFIITPITPKLIHKFGARNTAIGAG
                     IFGGIAYTLMFIIGYAPFGSYSGAERYKNFSGGSGVANMVYLTVMLTICGLPNCIIRV
                     CQAVLQGDVYDYSEWKYGVRNEGLVATVSNYFTKLANAVTGLLSGLVITFVGYTSHKD
                     ALGNVLRDTSPKVLMGFF"
BASE COUNT          742 a          629 c          594 g          786 t
ORIGIN      
        1 gcttataaca tcaagctttt taagggtttt atgaatggtg agccatcgga gattcgaact
       61 ccggacacct tgattaaaag tcaagtgctc tgccaactga gctaatggct catataaaaa
      121 gcattttacc gcttttatat tttagaaaaa gagaagcaga agtgtggcac cgagtgcaag
      181 cacaaaaaag aataaaccga agtatttcgt gaatctgctg agcttagcct ccatggttct
      241 gcctttattc ttaccgaaga aggtatcggc accgccggcg attgcaccgg aaaggcctgc
      301 ctggcgactc tcctgcataa ggataacaac ggtcagtata acagatgata atataagtaa
      361 tacagcaaga attatttcaa gagccgacat tttcttcctc cgttcaaatt tgaattcgtt
      421 ttcagaatgc taaagcattc tatcatactg ggtgtaaaaa agcaagtctt ttttataatt
      481 tttttatatg ccgggaaaat tcattgcata tatcgccgat ttcggagcat attatttaat
      541 gcagaagctt ttacacataa agagcgtttg ccgtaaaagc ttttgtgccg cttgcagctt
      601 tcattgccgc gagcggtttt ttatcggctc gcataaaaag cattgtgaaa caacatcatt
      661 ggccggtcgt aatgctctct ccggatgaag tctcgctttc cgaagtctct gcggaaatac
      721 cggagttgta tatgtaattt gagcctttgt cggtatccgc cgcggcttca gtcgttgttt
      781 tctcaaaaag gcctgtataa ttttttatga tgccgaaccc ggttaaaatt gaaaaggcac
      841 ttaccaaagc aagcagcacc gccgccacgg tcaaaaacac cgcaagcccc ttttctgcgt
      901 ttttgcttcc gccgccgttc tttgctttga cagtttcttc aaacatcatc ggctgatttt
      961 cgccgtaaaa atatgtttcc tcgggtcttg cctcgcttcg ctttgtgccg ttcgaaaaat
     1021 catattgaaa ttcctcacag gcacgttttt tttgctcgcc cgcacgcggc aaagcatgct
     1081 tgctgccgta tgatgaattc ttttccgtat ccgtttcgag catgacacag cctccttgga
     1141 aatcaattta aataatttta gcattgtgcg cataaaaagt caattactgc cggtttttgt
     1201 aaaaaagttc gcgtgctttg cggcaatatt cgccatatga taaatataaa ataaattacc
     1261 gtaaaattat tgaattaagg aaatcattat gctaaaataa taaaaatatt ttatttttta
     1321 caaataattt ccgggatgtg atttttttgg gagaaaatgc caccagtaca actgttgctg
     1381 agccgtccga gcaggagctt gaaaaagagg tcaaaaaagc ctccgagcaa aacagacgtt
     1441 atatccgtcc gagcgagctc gtagcgtttt tgcttacgac cttcggtcaa aagaaccttg
     1501 accagtttgt aaacgcatac aggcagttct ttatgataag ctttctcgga atttcggggc
     1561 gcgcctacgg tcttatagtc tttatcgaat caatatacga cgccgttgac gactcccttt
     1621 cgggtcttat catagacagg acaagaaccc gttggggcag acttcgcccg tatatgataa
     1681 tcaccgtacc tatttggggt cttgccacgc tgatgctttt cacaacgccg cagttcctct
     1741 cgggtaacac atcaaaaata gtttgggcgg taattgcaat attcgcatac aatctcggta
     1801 tgtcatattt caacgcctgg caaatacttc tctacaacat caccccgagc ctgcgagaga
     1861 gagacaacct catagcgact tcaaagttct ttgagctttt cggcacgtgg attccgtctt
     1921 tggtgccgct gttcgtttcg tttatgccga agatttcgga aagtatcggt atgcaggacg
     1981 tctactccgg atttgccgtt gccatgatgg taatagccgg cggcacggcg atatacggct
     2041 tcttcgcaat gagagaaaga gtgccgctcg cctcacgtga acaaatggag gaaatcagcg
     2101 ttctcgactc gtttaaaaat gcaattaaga acaagccgat gtttattctc atgctcgcaa
     2161 actttttcaa cggattcaaa tcagtcggag cctctaccga aagcttcttc tggcttcaca
     2221 acacgggcag ccttgcaaac ggcacaatag ccggcgtatt cacgggcatt ccgaacttca
     2281 taataacgcc tatcactcct aagcttatac ataaattcgg tgccagaaac accgcaatcg
     2341 gcgcaggtat tttcggcggc atagcataca cgctgatgtt cataataggt tatgctccgt
     2401 tcggaagcta ttccggtgcg gaaagataca agaacttcag cggcggcagc ggagtcgcaa
     2461 atatggttta tctcaccgta atgctgacaa tatgcggtct gccgaactgt ataatccgtg
     2521 tttgccaggc ggttttgcag ggcgatgttt acgattacag cgaatggaaa tacggtgtcc
     2581 gcaatgaggg ccttgtagca acggtatcaa actattttac aaagcttgcc aatgccgtta
     2641 ccggacttct ctcgggtctt gtcataacct ttgtcggcta cacctcacac aaggacgcac
     2701 tcggtaacgt actacgtgac acaagtccga aggttctcat gggatttttt g
//
LOCUS       DBMDVO010000104         2734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17327_length_2734_cov_1.124193, whole genome shotgun sequence.
ACCESSION   DBMDVO010000104 DBMDVO010000000
VERSION     DBMDVO010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17327_length_2734_cov_1.124193"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="ACZPE2_02310"
     CDS             complement(<1..340)
                     /locus_tag="ACZPE2_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318712.1"
                     /translation="MAVKEKKKKPQVEYEKQPESPTKKFDVTYAIVSVIAIIVLFSVL
                     VTAYYKNNYIMFSPEKVAEQYVENTAENDGYDALKYTVLIKNTKFGNFISENYMDKYI
                     DENATAPTMTA"
     gene            complement(343..1809)
                     /locus_tag="ACZPE2_02315"
     CDS             complement(343..1809)
                     /locus_tag="ACZPE2_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318713.1"
                     /translation="MKRYMRKSEIWTFGIGLFGVSLLTGWMPDYTYTFFNDFAFKGSG
                     IDPASITKMLAYVFGFAGIVGAVCELVIGYLVDRTRTKWGKIRPWIGFGVVPMAIVAM
                     LVFIAPKTGNYKLAVAWMFIIYSLYTAAACAVESPAGCFGACCTPNPEERGDAISIAS
                     IFKAIGQSGGMAVLPAVGFAMKAIMGKQQYKTAEGQGLDLVISTAVCALGCVLFTMIF
                     FPNNRERVPYTEEKVSLKESLKFVFTNKNLLMVSLTKFTGFGRGVYSTVSLYIAVYLL
                     GSKDLKIALLLPMGIGTAVGMLIVKKLLKMLDTKKVYIVCCLYGALSLTVLFLVSKAI
                     GFVPKELTIPFLIINFFVGLQHGNTNLTPNVMIADCVDEIEWKTGKRQEGLCYAGFGF
                     FSKVAAALTKSFGPALVVWAGYVTSTNANIAYATQSQTTLNRFLMIYTIIPAIFVLGQ
                     MIPIFFYDLNGKKRDMIAKELQERREKAAALKAAGEEA"
     gene            complement(1953..>2734)
                     /locus_tag="ACZPE2_02320"
     CDS             complement(1953..>2734)
                     /locus_tag="ACZPE2_02320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1318714.1"
                     /translation="FYSCGGDGTLYEVVNGAYKYPNAEVASLPLGSGNDFARLFGKRE
                     DLTNLDSQVNGSVHKLDLIKCGDKVAVNQCSMGIDAEVCAKQAYFKKIPLITGEAAYT
                     ASLLYCLGKRLNSEFKVTVDDDRVIEGTMLFALCGNSRWYGGGYMGAPKAIPDDGLLD
                     FIIVRKTVGRLKLAGLINAYKRGEHLDWDFTTFLRGKKMHIESKELAAVNVDGECEYV
                     KESTFEIMPSALNFVIPANSTFYENTGRPSPKRDEKELAVL"
BASE COUNT          704 a          645 c          630 g          755 t
ORIGIN      
        1 cggcagtcat tgtgggagcc gtggcatttt cgtcgatgta tttgtccata tagttctcgg
       61 agatgaaatt gccgaacttg gtgtttttta taagcacggt atatttaaga gcgtcatagc
      121 cgtcgttttc ggcggtgttt tcaacatact gctcggcgac cttttcgggt gagaacatta
      181 tgtaattgtt tttataatac gcggtgacga gaaccgagaa taaaacgatt attgcgatta
      241 ccgaaactat tgcatacgtt acgtcaaatt tctttgtggg actctcgggc tgcttttcgt
      301 attcgacctg aggctttttc tttttttctt taacagccat ttttatgcct cctctcccgc
      361 agcttttaac gcagcggctt tttcgcggcg ttcctgaagc tcttttgcta tcatatcacg
      421 ctttttaccg ttgaggtcat agaagaatat cggaatcatc tgccccaaaa cgaatattgc
      481 cggtattatc gtgtatatca taaggaatct gttaagggtt gtctgtgact gagtggcata
      541 agctatattt gcgtttgtag aggtaacata gcccgcccat acaacaagcg cgggaccgaa
      601 agactttgtg agggcggcag caacctttga gaagaagccg aagcctgcgt agcaaagccc
      661 ctcttgacgc ttgccggttt tccattcgat ttcgtcaacg cagtcggcaa tcattacgtt
      721 gggcgtaaga ttggtgttgc cgtgctgaag tccgacaaag aagttaatga taaggaacgg
      781 tattgtgagc tccttgggaa caaagcctat tgcctttgat accaaaaaca gtaccgtgag
      841 cgagagtgcg ccgtaaaggc agcatactat gtaaaccttt ttggtatcaa gcattttaag
      901 gagcttttta actataagca tgcctaccgc cgtgcctatg cccataggca acaaaagtgc
      961 tattttgagg tcctttgagc caagcaggta aactgctatg tagaggctta cggtactgta
     1021 tacgcctctg ccgaagccgg tgaatttcgt aagcgaaacc ataagaaggt ttttatttgt
     1081 gaatacgaat ttaaggctct ctttaaggct gactttttcc tcggtataag gaactctctc
     1141 acggttgttg gggaagaata tcatcgtaaa gagaacgcag ccgagcgcgc agaccgccgt
     1201 tgaaatcaca aggtcaagac cctgtccctc tgcagtttta tactgctgct tacccattat
     1261 ggctttcatg gcaaagccca cggcaggtaa aaccgccata ccgccggact gaccgatggc
     1321 tttaaatata cttgctattg agattgcgtc gccgcgctcc tcggggttcg gtgtacagca
     1381 cgcaccgaag cacccggcgg gactttctac ggcgcacgcc gccgccgtgt aaagagaata
     1441 tattataaac atccaggcaa ccgcaagctt gtaattgccg gttttgggag caataaatac
     1501 gagcattgca actattgcca tcgggacaac gccgaagcct atccaaggac ggattttgcc
     1561 ccatttggtt ctcgtcctgt caacgagata gccgatgaca agctcacaaa ccgcgccgac
     1621 tattccggca aagccgaata cataggcgag cattttggtt atggaggctg ggtctatacc
     1681 gcttccctta aatgcgaaat cattgaagaa cgtataggtg taatcgggca tccagccggt
     1741 gagaagactg acgccgaaaa gacctatgcc gaaagtccat atttcggact ttctcatgta
     1801 ccttttcata aaagacctcc ttaataaaaa gaaaagggtg ccgaagtgtc agcacccttc
     1861 aaagacataa caaaaattat atcccaaaaa ataatataat tcaactgata tattttacaa
     1921 aaacgacagt aaaaatttta caatttaatg cgttaaagaa cagcaagctc tttttcgtct
     1981 cttttgggag acggacggcc tgtgttttcg tagaaggttg aatttgcggg aattacaaaa
     2041 ttcaaagcgg acggcattat ttcaaacgtg ctttctttaa cgtactcgca ttctccgtca
     2101 acgtttactg cggcaagctc cttgctttct atatgcattt ttttaccgcg caaaaatgtg
     2161 gtaaaatccc aatcgagatg ctcaccgcgc ttatatgcgt ttatgagccc cgcaagcttc
     2221 aatctgccta cggttttgcg gactattata aagtcgagca ggccgtcgtc gggaatggct
     2281 ttcggcgcac ccatatatcc tccgccgtac cagcgcgaat ttccgcagag cgcaaacagc
     2341 attgtaccct ctatcaccct gtcgtcgtca acggttacct taaattcgct gttgaggcgc
     2401 ttgccgaggc agtagaggag cgacgcggta tatgcggctt cgcccgttat gagcggtatt
     2461 tttttgaaat aagcctgctt tgcacagacc tctgcgtcaa ttcccattga gcactgatta
     2521 actgcaacct tatcgccgca tttgatgagg tcaagcttat gtacgctgcc gtttacctgc
     2581 gaatcaaggt ttgtgaggtc ttctctcttt ccgaaaagtc ttgcaaaatc attacccgag
     2641 cctaagggca gagacgcgac ctcggcgttc gggtacttgt atgctccgtt tacgacctca
     2701 tacaacgtgc cgtcgcctcc gcaggaataa aacc
//
LOCUS       DBMDVO010000105         2731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17359_length_2731_cov_1.158935, whole genome shotgun sequence.
ACCESSION   DBMDVO010000105 DBMDVO010000000
VERSION     DBMDVO010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17359_length_2731_cov_1.158935"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /gene="pflB"
                     /locus_tag="ACZPE2_02325"
     CDS             complement(<1..2043)
                     /gene="pflB"
                     /locus_tag="ACZPE2_02325"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1318715.1"
                     /translation="MEFSRAWEGFNPGEWNEKVNVSDFIKRNYTEYTGDSSFLSGATE
                     RTKELMEKFSKLLDEERENGGVLSVDTETVSSLVTYAPGYLDKEKELIVGLQTDSPLR
                     RGVNPFGGMRMAREAAKAYGYELSDKIEDEFRYKTTHNDGVFRAYTSEMRKARHIALL
                     TGLPDAYGRGRIIGDYRRVALYGMDTLIEFKKADKIALENKDMTEEVIRLREELQKQL
                     NFMEMLKQMAQMYGYDISSPASNAREAIQWLYFAYLAAIKEQNGAAMSLGRTSTFLDI
                     YIERDINRGVLTESGAQELIDDFVMKLRMARHLRTPEYNELFGGDPMWITESIGGMTN
                     EGKTLVTKNSYRMLNTLYTLGSAPEPNMTVLWSQGLPENFKRFCAEVSEDTDSIQYEN
                     DDVMRPIYGDDYAIACCVSAMQVGKQMQFFGARCNLAKLLLLALNGGYDNVYCEKIGP
                     QTNPIEGDRLDYDEVVERYKTYLKWLCRLYVNTMNVIHFMHDKYAYEKIQMALHDTDV
                     HRFMAFGVAGLSVAADSLSAIKYANVKPIRDENGYIVDFETTGDFPKYGNDDDRVDNI
                     AKALLEDTIAELRKTPAYRNAEHTLSALTITSNVVYGKKTGSTPDGRKKGEPFAPGAN
                     PMHNREKNGALASLNSVAKMPYSQCRDGISNTFSIVPAALGKTRDERTANLVSILDG"
     regulatory      complement(2122..2217)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(2279..>2731)
                     /locus_tag="ACZPE2_02330"
     CDS             complement(2279..>2731)
                     /locus_tag="ACZPE2_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318716.1"
                     /translation="VTMNSTTEKQISSVMNSLLFIVVILIVAAGLLAFVVLYNLNNIN
                     ITERRRELATLKVLGFYNKELAFYVYRENIVLTIFGVIFGIVLGIILHAYVMSTIETD
                     YIMFGKEMTVISYLASILLTVLFTAIVNFVMYFRLKKIDMVESLKSAE"
BASE COUNT          665 a          699 c          612 g          755 t
ORIGIN      
        1 gccgtcaaga atcgacacaa gatttgccgt tctctcgtct ctcgttttgc cgagtgctgc
       61 tggaacgatt gaaaacgtat ttgaaattcc gtcgcggcac tgtgaatagg gcatcttggc
      121 aaccgagttg agcgatgcga gcgcgccgtt cttttcgcga ttatgcatgg ggttggcacc
      181 gggagcaaac ggctcgccct tctttctccc gtcgggcgtg gagcctgtct ttttgccgta
      241 cacgacattg gaggttattg tgagcgccga aagcgtatgc tctgcgtttc tgtaagccgg
      301 agtcttgcga agctcggcta ttgtgtcctc aagcaatgct tttgcgatat tgtcaacacg
      361 gtcgtcgtcg tttccgtact tcgggaaatc acctgtcgtc tcaaaatcga ctatatatcc
      421 gttctcgtca cgtatgggct taacgtttgc gtacttgata gccgaaagag agtctgcggc
      481 aaccgagagt cccgctacgc cgaatgccat aaagcggtgc acatcggtat cgtgaagtgc
      541 catttggatt ttctcatacg catacttatc gtgcataaaa tgaattacat tcatagtatt
      601 gacataaagc ctgcaaagcc atttaaggta agtcttgtat ctctcaacca cctcgtcgta
      661 atcaagcctg tcgccctcaa tcgggttagt ctgcggacct attttttcgc agtaaacatt
      721 gtcatatccg ccgtttaatg cgagaagcaa aagctttgca aggttgcagc gcgcaccgaa
      781 aaactgcatc tgcttgccta cctgcatagc ggaaacgcag caggctatgg cgtaatcgtc
      841 gccgtaaatc gggcgcataa cgtcgtcgtt ttcatattgt atggagtcgg tgtcctccga
      901 aacctcggcg caaaaacgtt taaagttttc gggcaagccc tgcgaccaga gaacagtcat
      961 attgggctcc ggagccgagc cgagagtgta aagcgtattg agcattctgt acgaattttt
     1021 cgtgacgaga gttttaccct catttgtcat accaccgatg ctctcggtta tccacatagg
     1081 gtcgccgccg aacagctcat tgtattcggg agtgcgaaga tggcgagcca tgcgcagctt
     1141 cataacaaaa tcgtctatca gctcctgagc accgctctca gtgagtacgc ccctattgat
     1201 gtcgcgttca atatagatgt caaggaacgt gcttgtcctg ccgagcgaca tagccgctcc
     1261 gttttgctcc ttgattgcgg caagatatgc aaagtaaagc cactgaatcg cctcgcgcgc
     1321 gttgcttgcc ggcgaagata tgtcgtagcc gtacatctgc gccatttgct tgagcatttc
     1381 cataaagtta agctgttttt gcagctcctc gcgcagcctt atgacctcct ctgtcatatc
     1441 cttattttca agcgcgattt tatctgcctt tttaaattcg ataagcgtat ccataccgta
     1501 gagggcaact ctgcgatagt ctcctattat tctgcctctt ccgtaggcgt cgggcaaacc
     1561 ggtgagcagc gcaatatggc gtgcctttct catctcactc gtataggctc tgaatactcc
     1621 gtcgttgtga gttgtcttat atctgaactc gtcctcgatt ttatcggaca gctcatagcc
     1681 gtaagccttt gccgcctcac gcgccatacg cataccgccg aagggattta cacctcggcg
     1741 cagggggctg tctgtctgaa gacccacgat aagctccttt tccttgtcaa gataaccggg
     1801 cgcataggtg acgagagatg aaacggtttc ggtatcgacc gacaaaactc cgccgttttc
     1861 acgttcctcg tcaagaagct ttgaaaactt ttccataagc tcctttgtac gctcggtggc
     1921 tccgctcaaa aatgagctgt cgcccgtata ttccgtgtaa ttccttttga tgaaatcact
     1981 gacgtttact ttttcgttcc attcgccggg attaaagcct tcccacgctc ttgaaaactc
     2041 catttgccat cttcctttca cagatgcctc gaggcatata tacataaata aattacaata
     2101 taaaagggca gcccccgaaa aagaggctgc ccagacggtc ggctttttat acgccgcgtt
     2161 ccccggcagt ttagcctgcc tgcccaaatg ggctttccgt cagttacacg gcgcctatat
     2221 attgtggtgc tattatatca ctacacaata tatatgtcaa catataaatt aaaaatgttt
     2281 attctgcgct cttcagcgat tcaaccatat ctatcttttt aagcctgaaa tacataacga
     2341 agttgactat cgccgtaaac aacacagtaa gcaaaatact tgcgagatag ctgataaccg
     2401 tcatttcctt accgaacata atatagtccg tctcaatggt gctcataaca tacgcgtgaa
     2461 gaattatacc cagaactata ccgaatatca caccgaaaat cgtcagtaca atattttcgc
     2521 ggtacacgta aaaggcaagc tccttgttgt agaaaccgag aaccttgagc gttgcaagct
     2581 cacgcctgcg ctcggtgatg ttgatgttat tgaggttata tagaaccaca aacgccaaaa
     2641 gccccgcagc aactatcagt attacaacta taaacagcag gctgttcata actgagctta
     2701 tctgtttctc ggtggtgctg ttcatggtaa c
//
LOCUS       DBMDVO010000106         2720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17462_length_2720_cov_1.386407, whole genome shotgun sequence.
ACCESSION   DBMDVO010000106 DBMDVO010000000
VERSION     DBMDVO010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_2720_cov_1.386407"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="ACZPE2_02335"
     CDS             <1..398
                     /locus_tag="ACZPE2_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017016.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1318717.1"
                     /translation="RGVNVLEKNKVKVTIAGVDYALTTEETPEYTTMLAAEIDEKMKE
                     LRAVNPFISTNQAAVLIALDYANSAKKAEQSADNLRAQIKDYLEDASQSKSERDFYKR
                     EIDRLKTEQKAGAAQINLFSSEKEPDSAK"
     gene            395..>922
                     /locus_tag="ACZPE2_02340"
     CDS             395..>922
                     /locus_tag="ACZPE2_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1318718.1"
                     /translation="MIKPEILAPAGSFEALTAAVRSGADAVYFGTGNFNARRNASNFS
                     GDDLQRAVDFCHERNVKVHITLNTLVKDSEMSELKESVRRICKSGADALILQDLGVLR
                     VVKDICPDIELHASTQMSVGTLDGIRTLADLGFSRAVLPRELSKDEIKYLCENSPIEL
                     EMFVHGALCMCVSGQC"
     assembly_gap    923..932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <933..2400
                     /locus_tag="ACZPE2_02345"
     CDS             <933..2400
                     /locus_tag="ACZPE2_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1318719.1"
                     /translation="RGLCAQPCRLPFAAENGTGHDLSLKDLSLIEYAPILSKMGISSF
                     KIEGRMKRPEYVAAAVTALKNSLSGEYSSENAEDLCSLFSRSGFTKGYYENALGRNMF
                     GFREKENVTSATASLLKKYERIYEKERAVYRVHFVLSVKSDKKISLMASANDLSVTVL
                     SENLPQKAVNRPFTKEEALLRLKKCGGTVFSFGDADICIDDGLSVSAAEMNALRREAL
                     LRLADRLKERAPYRINKANIIFRNRKPNKKPQTYISFRDTSAIPQNLECDKLFIPLSS
                     KPELFEKYSAGAVLPRGIFGNFDEIVERLIKSGARYALCNTLDSVAAAKKAGVQIIGG
                     PFLNIFNSVSLSEIQKLGAAECVLSYEMTLKQLTALEGECRRGVCVYGRVPLMLTRNC
                     PVKNGKSCRECGGKSFLRDRKGIEFPVRCTNGFSELFNSRPIYMADRMNEVKNADFVL
                     LEFTTEDKEQISSVLKAYQNGSSPEIEFTRGLLYRGVE"
BASE COUNT          769 a          575 c          664 g          702 t
ORIGIN      
        1 acaggggtgt gaatgtcttg gaaaaaaaca aggttaaagt aactattgcc ggagttgact
       61 atgctctcac gactgaggag actcccgaat atactactat gctcgctgcc gaaatcgacg
      121 aaaagatgaa agagcttcgc gctgtaaatc cgtttatttc aacgaatcag gcggctgttt
      181 tgatagcact tgattatgcc aacagtgcta aaaaggcaga gcagagcgcc gataatctcc
      241 gtgcccagat aaaggattat cttgaggatg cgtctcagtc gaagtccgaa agagacttct
      301 ataagcgcga gatagaccgc cttaaaacag agcaaaaagc cggagcggcg cagataaatc
      361 tgttctcttc cgaaaaagag ccggattccg ctaaatgata aagcccgaga tattggctcc
      421 cgcaggcagc tttgaagcat taactgccgc ggtaagaagc ggagcggacg ccgtgtattt
      481 cggcaccggc aactttaacg cgcgcagaaa tgcctcgaat ttttcggggg acgatttgca
      541 gcgcgcggtg gatttttgcc acgaaagaaa tgttaaagtt catataacct taaatactct
      601 tgttaaagac agcgaaatgt cggagcttaa ggaaagcgtc aggcgtatct gcaaaagcgg
      661 tgcagatgcg cttattttgc aggacctcgg cgttttgcgc gtcgtaaagg atatttgtcc
      721 cgatatagag ctgcacgcct ccacccaaat gtcggtggga acgttggacg gcatacgtac
      781 tcttgccgat ttaggattct caagagctgt tttgcctcgc gaattatcaa aagacgagat
      841 taaatatctg tgcgaaaact cgccgattga gcttgaaatg ttcgtccacg gggcgctttg
      901 tatgtgcgtt tccgggcaat gtnnnnnnnn nnccgcggtc tgtgcgctca gccctgccgg
      961 cttccttttg cggcggaaaa cggaacgggg catgatttga gcttaaagga tttgtcgctt
     1021 atcgagtacg caccgatatt gtctaaaatg ggaatatcat ctttcaaaat agagggcaga
     1081 atgaaacgtc ccgagtatgt cgcagccgca gttacagctc tgaaaaattc gctgtcgggg
     1141 gagtattcaa gcgagaatgc cgaagatctt tgctccctgt tttcacgctc cggatttaca
     1201 aagggatatt acgaaaatgc tctgggcaga aatatgttcg gtttccgcga aaaggaaaat
     1261 gtaacctctg ctactgcctc gcttctcaaa aaatatgagc gtatttacga aaaagaacgt
     1321 gcggtttaca gggtgcattt tgttctctct gtgaaatccg ataagaaaat cagccttatg
     1381 gcttccgcaa atgatttaag cgttactgtt ttatccgaaa acttgccgca aaaagccgta
     1441 aacagacctt ttaccaaaga agaggctctt ttgagactta aaaaatgcgg cggaaccgta
     1501 ttttctttcg gcgatgccga catctgtata gacgacggct tgagtgtgtc ggcggcagaa
     1561 atgaatgcgc tccgcaggga ggctctgctt cgccttgccg acaggcttaa agaacgagca
     1621 ccgtaccgca taaacaaagc taatattatt ttccgaaatc gcaagccgaa taaaaagccg
     1681 cagacgtata tttcgttccg cgatacatcc gccattcccc aaaatttgga atgtgataag
     1741 ctttttattc cgctgtcttc aaagccggag ctttttgaaa agtattccgc aggtgcagtt
     1801 ttgccgaggg gtatattcgg taattttgac gagattgtcg agaggcttat aaaaagcggc
     1861 gcaaggtatg ccctttgcaa tacgctcgat tcggttgcag cggcaaaaaa ggcgggtgtt
     1921 cagataatag gcggaccgtt tttaaatatt tttaactcgg tttctctgtc cgaaattcaa
     1981 aagctcggag cggcggaatg tgtgctctca tacgaaatga ctttgaaaca gctgactgcg
     2041 cttgagggag aatgtcggcg cggtgtttgc gtctacggca gagtaccgct tatgctcaca
     2101 agaaattgtc cggtaaaaaa cgggaaaagc tgtcgcgagt gcggcggaaa atccttttta
     2161 cgcgacagaa agggcattga attcccggtt cgctgtacaa atggattttc cgaactgttc
     2221 aattcaagac ccatatatat ggctgacaga atgaatgagg tcaaaaacgc ggattttgtg
     2281 cttttggagt ttacaaccga ggacaaagaa cagataagct cggtactcaa ggcgtatcaa
     2341 aacggttctt cgcccgaaat tgaatttacc cgcggacttt tgtaccgcgg cgtggaataa
     2401 acgcaaagaa ttttttatgt gtaaaaataa aacggagctg taaacccata cgtcttacgc
     2461 tgagtgctta taaggcagta ttgcttcaaa ataacatctt ttagcataac ccctcagata
     2521 agcccaaact tatctgaggg gtgcattttc ttgtatcaaa aatatatcat tttgaagtgc
     2581 ttcgcagttt catagctata aaaatttttt atgccgaagt caagaaatga agcaaggctg
     2641 tcgccttgcg aggctttttg acaaaagcat aagagatttt tatgcgagaa accaatactt
     2701 ccttatgaac gctcagcctt
//
LOCUS       DBMDVO010000107         2688 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17781_length_2688_cov_0.865868, whole genome shotgun sequence.
ACCESSION   DBMDVO010000107 DBMDVO010000000
VERSION     DBMDVO010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17781_length_2688_cov_0.865868"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="ACZPE2_02350"
     CDS             <1..243
                     /locus_tag="ACZPE2_02350"
                     /inference="COORDINATES: protein motif:HMM:NF018435.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1318720.1"
                     /translation="DVLEKTPPELVGDISDNGVTLTGGTSLLWGMDKLVENETGIETK
                     IAEDAMYSTVNGAAAAINDIEYLTNNGYTFQTVREV"
     gene            complement(244..1200)
                     /locus_tag="ACZPE2_02355"
     CDS             complement(244..1200)
                     /locus_tag="ACZPE2_02355"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1318721.1"
                     /translation="MNTYGFTKKIALVGTGFVGMSFAYAMLCESVCDELVLIDVNKQK
                     AEGEAMDLNHGLSFAKTNMKIYAGSYTDCRDADIVVICAGVGQKPGEDRLSLLQRNTE
                     VFKTIISPIIYSGFNGIFLVATNPVDIMTKITWQLSGFDCKKVIGTGTTLDTARLRYL
                     LGDYFNVDPKNVHAYVIGEHGDSEMVPWSQAFVGTKSIMSIVENNPDRFKLTDLDEIA
                     KDVTGAAQKIIEAKKATYYGIGMALVRIVKAIFGDEKSVLTVSSMFRGEYDINGVYAG
                     IPSIIGRDGVREIVTLDITAEEEKKLKESCELLKNASEGIKI"
     gene            1529..>2688
                     /locus_tag="ACZPE2_02360"
     CDS             1529..>2688
                     /locus_tag="ACZPE2_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR1318722.1"
                     /translation="MVLTKEQQALLDGEKGETMAKVMKTLVMYGEAFGAERMVPVTSK
                     YGHTVISFGIGVMKPIYELYDTLLEAGAVSKQPFSADPRPLDKNVPSSFLQDIVFKFM
                     YSQQERYETQLKHFGLMGDKAYTCACYLPEVGNTPKRGEILSWAESSAVVYANSVLGA
                     RCNRNSGIIELMGSIAGFVPEFGLLTDEGRKADWIIEIKTTKKPEAQILGSAIGMKVM
                     EKVPYIRGLDKWIGTELNDEACAYLKDFGAATASNGSVGLYHIENLTPEAVDYGKKLL
                     RDNAPTYIIDDAELERVKNSYPCIWKDPNATPKLCFMGCPHMTLKQLEMWTERVENGL
                     KESGNKKVLVPTVFTSAPDIIEEFNKTEYAERLKNTGVILSYICPLMYMNNP"
BASE COUNT          785 a          519 c          572 g          812 t
ORIGIN      
        1 gatgtgttag aaaaaacacc tcctgagctt gttggagaca tttcggataa cggagttact
       61 ttgacaggtg gtacttctct tttgtgggga atggataagc ttgttgaaaa cgaaaccggt
      121 atagaaacaa aaattgccga ggacgccatg tactcaacag ttaacggcgc cgctgcggca
      181 atcaatgata ttgaatattt aaccaataac ggttatacat ttcaaacggt acgcgaagta
      241 taatcagatt ttaatgcctt cgcttgcatt tttgagaagc tcacaggatt ctttgagctt
      301 cttttcttct tcagctgtta tgtcaagagt aactatttct ctgactccgt ctcttcctat
      361 aatgctcggt ataccggcat atactccgtt gatgtcatat tctccgcgga acattgaaga
      421 aacggttaat acgctttttt catcgccgaa tatagctttt acgattctta ccaaagccat
      481 tcctattccg taataggtgg ctttttttgc ttctattatt ttttgtgcag ctcctgtaac
      541 atcttttgct atttcatcaa ggtcggtaag cttgaatcta tccggattgt tttcaacaat
      601 gctcattatg ctttttgtgc cgacgaaagc ttgcgaccaa ggtaccatct cgctgtctcc
      661 gtgctcgccg ataacgtatg cgtgaacatt tttcgggtca acgttaaaat aatcaccgag
      721 aagataacga agtcttgcag tatcaagcgt agtaccggtg cctataactt ttttgcagtc
      781 gaatccggaa agctgccatg taatctttgt cataatgtca acaggattgg ttgcaacaag
      841 gaaaattccg ttaaaacccg aatatattat aggagaaatt attgttttga aaacctctgt
      901 gtttcgttga agcaacgaca gcctgtcttc tccgggcttt tgacctacac cggcgcatat
      961 aactactatg tccgcatctc tgcaatcggt ataagagcct gcatatatct tcatatttgt
     1021 tttagcaaag gataaaccgt gattaagatc catggcttct ccttcagctt tttgtttgtt
     1081 tacatcaatc aacacaagct cgtcgcaaac actttcgcaa agcatagcgt atgcaaaact
     1141 cattcctaca aaaccggtgc caaccaaggc tattttcttt gtaaatccgt atgtgttcat
     1201 ttgtttcatc ctttctttga ttgctgttgt acgtattttg tacgtgttgc atttttagta
     1261 tatcattaag catgtaaaaa ataaacaaaa ttcacttgtt taggaaaagt ttcagcatat
     1321 tattcatatg caagagaaaa aaacaattta tactattgaa tattatgaat aaatgatgca
     1381 aaatccgcgg attttttacc gccgctattc ttttatattg aaatccggca tcgcttgtga
     1441 tataattaac aaaatgttga tactgagcaa gtgtaattta tataatttgc tgcaattaac
     1501 gcaaaaatat aaaaaggggt tgttatttat ggtcttaaca aaagagcaac aagcactttt
     1561 ggacggcgaa aaaggcgaaa ccatggcaaa ggttatgaaa actcttgtta tgtacggtga
     1621 agccttcggc gcagagcgta tggttcccgt tacaagcaag tatgggcata cagttatcag
     1681 cttcggaata ggggttatga agccgatata tgagctgtat gatactcttc ttgaagccgg
     1741 tgctgtcagt aagcagccat tttcggccga cccgagaccg cttgataaaa acgtgccgtc
     1801 atcatttttg caggacattg tatttaagtt tatgtacagt cagcaagaga ggtatgagac
     1861 acagctcaaa cacttcggat tgatgggtga taaggcgtat acgtgcgcct gttatcttcc
     1921 ggaggttggt aatactccta aacgcggtga aattctgtcc tgggctgagt cctcggctgt
     1981 tgtatatgcc aacagcgttc tcggagcgag gtgcaacaga aattcaggta ttattgagtt
     2041 gatgggttca atagcaggat ttgttcctga atttggattg cttaccgatg aaggcagaaa
     2101 agcagattgg ataatagaaa ttaaaacaac caaaaagcct gaggcccaga tactcggttc
     2161 tgccatagga atgaaggtaa tggaaaaagt accttatata aggggccttg ataaatggat
     2221 aggaaccgaa ttgaatgacg aggcttgtgc ctatctcaag gatttcggtg ccgcaacagc
     2281 atctaacggc tctgtgggtc tgtatcatat agaaaacctg acgccggaag ccgttgacta
     2341 cggtaaaaag cttttaaggg ataacgcgcc tacatacatt attgatgacg cggagctcga
     2401 gcgagttaaa aacagttatc cttgtatttg gaaagacccg aatgcaacgc ctaagctttg
     2461 ctttatgggc tgtccgcata tgacattgaa acaacttgaa atgtggacgg aacgcgttga
     2521 aaacggctta aaggagtcag gaaataaaaa ggttcttgtt ccgacggtat ttacatccgc
     2581 tccggatata atagaagagt ttaacaaaac cgagtatgcc gagcgactta aaaataccgg
     2641 cgttatattg agctatattt gcccgcttat gtatatgaat aatccgtt
//
LOCUS       DBMDVO010000108         2687 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17789_length_2687_cov_0.967517, whole genome shotgun sequence.
ACCESSION   DBMDVO010000108 DBMDVO010000000
VERSION     DBMDVO010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17789_length_2687_cov_0.967517"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="ACZPE2_02365"
     CDS             <1..664
                     /locus_tag="ACZPE2_02365"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR1318723.1"
                     /translation="VGIAHPYELGSPMCTGCFWDYHVKKWENVDYIEVWSKPFPPSKS
                     ANERAFSLWTGLLDKGYHLAATYGKDWHKKSDETEPYGCTYIGTESETLTGAEIKKAV
                     QNGRTSITMGPLITLTAQRSDAEYNVGDVLEEGKAKIKIEVFPNTRKEHWEKFNITLE
                     SVRLIRNGRRTVFESKYGGDALSFTLNCEPGWYIAELWGKINGQRYMIGFTSPMYFTV
                     KK"
     gene            complement(748..1128)
                     /locus_tag="ACZPE2_02370"
     CDS             complement(748..1128)
                     /locus_tag="ACZPE2_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018621.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HIR1318724.1"
                     /translation="MSKFLIRPHHLLCMRYFKGCGYSDEFTENMRYVISNLKNGSEVL
                     LTRGCDDICRKCPNNRKNMCCDGEKVKSYDSAVISSCNLDFDKVYEYGCLYSLVENNI
                     IKKDMLRFVCGDCQWFHICGIARV"
     gene            complement(1109..1684)
                     /locus_tag="ACZPE2_02375"
     CDS             complement(1109..1684)
                     /locus_tag="ACZPE2_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1318725.1"
                     /translation="MAEKEIKAKRSNRQKTLDMVYIAIFAVIIAVCSWISVPMVVPFT
                     LQTFGVFCAVGLLGGKRGTLSVLIYVLLGAVGVPVFAGFSSGIGTLFGVTGGYIMGFI
                     FTALIYWLITAIFGKKLWAIILGMVLGLLACYAFGTIWFMVVYAQKAAPIGLAAALMK
                     CVVPFLIPDALKIALAVVITKTVPKYVKIFD"
     gene            complement(1753..>2687)
                     /locus_tag="ACZPE2_02380"
     CDS             complement(1753..>2687)
                     /locus_tag="ACZPE2_02380"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1318726.1"
                     /translation="TFLGTPLEKSCKAVVYQQNSDDKFVVVFIRGDLDVNETKLTNYL
                     CENIHPGVITEECGLKAGFIGPCNLSGDFRVVYDSSLKGTNNLSCGANKEGYHYTGLC
                     MERDVPDAEYVDVAKITAGGICPNCGKHTIKISRGIEVGNIFQLGTKYTKSMNMTYLD
                     KDGNAQYPVMGCYGIGVGRLAASVCEAHHDEYGPIWPMSIAPWQVHLCCLRSDDENAK
                     SFADSLYKDLQNDGVEVIYDDRNVRPGVMFSDADLLGVPVRVIVSPRNMAENVCEVVT
                     RDKSVSKKVPVSEVKETVEQMVRDMLKKYIVTQE"
BASE COUNT          766 a          644 c          590 g          687 t
ORIGIN      
        1 ggtaggcata gcacatccgt atgagctcgg aagcccgatg tgcaccggct gcttttggga
       61 ctatcacgta aaaaaatggg aaaatgtgga ctatatcgag gtatggtcaa agccgtttcc
      121 gccgtcaaaa tcggcaaacg agcgcgcctt ttcgctttgg acgggtcttc ttgacaaggg
      181 gtatcacctt gccgcaacct acggcaaaga ctggcacaag aaatccgacg aaacggagcc
      241 atacggctgc acttacatag gcaccgaaag cgaaacgctc accggtgcgg aaataaaaaa
      301 agccgtgcaa aacggaagaa cctcaattac catgggtcct ttgataacac ttacggcaca
      361 gcgaagcgat gctgaatata atgtaggcga tgttttggag gagggcaaag caaaaattaa
      421 aatagaagtt tttccgaata cacgaaaaga gcattgggag aaattcaaca ttaccttaga
      481 atcggtaagg ttaataagaa acggacgccg gaccgtgttc gagtcaaaat acggcggcga
      541 cgctctgtcc tttaccttaa actgtgagcc cggctggtac atagccgagc tttggggaaa
      601 gataaacggt caaaggtata tgataggatt tacaagtcct atgtacttta ccgttaagaa
      661 ataacaatac cgccgtgcaa aacgcaaaaa gctgctgtaa agaataagcc tttacagcag
      721 ttttttaatg ttcaatcgaa gtttttgtca cacccgtgct atgccgcata tatgaaacca
      781 ttggcagtcg ccgcatacaa agcggagcat atcttttttt atgatattat tttcgacaag
      841 cgaataaaga cagccgtact catacacctt gtcaaaatcg agattgcacg agcttattac
      901 agcactgtcg tagcttttaa ccttttcacc gtcgcaacac atattttttc tgttattggg
      961 gcatttgcgg cagatgtcat cacagccgcg agtcaaaaga acctcgctgc cgtttttcag
     1021 atttgatatt acataccgca tattttcggt aaattcatcc gaatagccgc agcctttaaa
     1081 atagcgcata cacaaaaggt gatgcggtct aatcaaaaat tttgacatat ttcggcaccg
     1141 tctttgttat aactacggcg agggcgattt taagcgcgtc cggtatcaaa aacggaacaa
     1201 cacatttcat aagggcggcc gcaagtccta tcggtgccgc tttttgcgca taaacaacca
     1261 taaaccaaat cgtaccgaag gcatagcaag ccaaaagtcc gagaaccatt ccgaggatta
     1321 tcgcccaaag ctttttcccg aaaatcgccg ttatgagcca atagataagt gcggtgaata
     1381 taaatcccat tatatatccg ccggtcacac cgaacagcgt gcctatgccg cttgaaaatc
     1441 ccgcgaatac cggaactccg acagctccga gtaatacata tatgagaacg gacaaggttc
     1501 cccttttacc gccgagcaag ccgacggcgc agaaaacacc gaaggtctga agcgtaaacg
     1561 gcactaccat gggcaccgag atccacgaac aaacggcaat tattacggca aatatcgcta
     1621 tgtataccat atcaagcgtt ttttgcctgt tgcttctttt agctttaatt tccttttccg
     1681 ccataaaaat actcctttaa gctgaaactt aaaggagtat aacattaacc gttttgattg
     1741 tcaaccaaaa aatcactctt gggtgacaat atattttttg agcatatcgc gaaccatctg
     1801 ctcaaccgtt tccttaacct cggaaaccgg aaccttcttg ctgacggatt tgtctcttgt
     1861 aacaacctcg cagacatttt cagccatatt tctggggctc actattactc ttacgggaac
     1921 accgagcaag tcggcatcgg agaacattac gcccggacgc acgtttctgt cgtcatatat
     1981 gacctcaact ccgtcattct gcaagtcctt gtaaagggag tcggcaaagc tcttggcgtt
     2041 ttcgtcgtcg gagcgaaggc agcaaagatg aacctgccac ggtgctattg acataggcca
     2101 aataggaccg tattcatcat ggtgagcttc acataccgac gcagcaagtc tgccgacgcc
     2161 tataccgtag cagcccatta cggggtactg cgcgttgccg tctttatcga ggtatgtcat
     2221 attcatactc tttgtgtatt ttgttccgag ctggaatatg tttcctacct cgatgccgcg
     2281 tgaaattttt atggtatgct taccgcaatt cgggcaaatg ccgccggcgg ttattttggc
     2341 tacatcaaca tattcggcat cgggaacgtc gcgctccata caaaggcctg tatagtgata
     2401 gccctcttta tttgcgccgc acgagaggtt gtttgtgccc ttaagcgagc tgtcataaac
     2461 aactctgaag tcgccgctta aattacacgg accgataaat ccggctttaa gaccgcactc
     2521 ctccgtaata actccggggt ggatattttc gcaaagataa ttagtaagct tggtttcgtt
     2581 tacgtcgaga tcgcctctta taaatacgac aacaaatttg tcatccgaat tctgctggta
     2641 cacaacggcc ttacatgact tttcaagcgg tgtgccgagg aaggtgc
//
LOCUS       DBMDVO010000109         2678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17866_length_2678_cov_1.120295, whole genome shotgun sequence.
ACCESSION   DBMDVO010000109 DBMDVO010000000
VERSION     DBMDVO010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17866_length_2678_cov_1.120295"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="ACZPE2_02385"
     CDS             <1..2054
                     /locus_tag="ACZPE2_02385"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1318727.1"
                     /translation="YSGQPYIIHPIAVSNILLDLGMDAQSVEAALLQDTVEDTDITLD
                     YIKHEFGDDVAALVDGVTKLGKVPLSNREEQQAENIRKMLLAMSHDIRVIIIKLADRI
                     HNMRTLSFRVPQKRRDTALETLEIYAPIAHRLGIRPAKEELEDLSIRFLDPIAYKEIE
                     ENLKRLSKSRLDFLESMEGKIRERITGVVKDPTIEGRIKSVHGIYRKMYMQNKNFAEI
                     YDVYAIRIIVDSVIDCYNCLGVIHDMFRPLPGRFKDYISTPKPNMYQSLHTTVIGKEG
                     IPFEVQIRTWEMHRTAEFGIAAHWKYKLGVHGKSNLDKRLAWIRQMLEDQQETGDVRE
                     IVQAIKSDLVPEDTFVFTPRGDVISLPAGATVIDFAYAIHTAVGNKMVGAKVDGRIVP
                     LDYVVKTGEIVEVLTSSQTGKGPSRDWLSIAKTSQARNKIRSWFKNERRDENIAEGKA
                     EVDREFKRNFIRLSDENYEKFVANLAERQHLRNVDDFYAAIGYGGISVIRLMPYIKEE
                     YNKNYRSQETVVLPEDDKIKTSRRKGGSDGVTVSGIDNCLIKFSRCCNPLPGDNIIGF
                     ITRGHGVSIHKRDCPNVPSDFSKCDEPQRWVAAAWNTDVKTEFKASLIITCLSRIGLM
                     ADVSVQLAAMKVNINEISSRETKDGRSQISMTITVSNVDHLKNVIARLKKVDGVLSVE
                     R"
     gene            2209..>2678
                     /locus_tag="ACZPE2_02390"
     CDS             2209..>2678
                     /locus_tag="ACZPE2_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024785.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1318728.1"
                     /translation="MKSYEKLTPEGTRDFLFEESDALRRIERGLSELFKEKGYKKVIT
                     PTVEFFDVFNRESAGTLPENLYSMTDAYGRLLVLRPDSTLPIARIAATRLKGAELPIR
                     LYYNQNVFSRRKKLTGRNDETSQSGIELIGVNGIRADIEVIETAIEALKVCGAP"
BASE COUNT          787 a          608 c          684 g          599 t
ORIGIN      
        1 gatattcggg tcagccgtat attattcacc ctattgccgt gtcaaatata cttcttgacc
       61 tcggtatgga cgcacaatcg gtagaggcgg cgcttttaca agatacggtc gaggataccg
      121 atataacctt ggattatatt aagcacgaat tcggcgacga tgttgcagca cttgtagacg
      181 gtgtcacaaa gctcggcaag gtgccgcttt caaatcgcga ggagcagcag gcggaaaata
      241 tcagaaaaat gctgcttgcc atgtcccacg acataagggt catcataatc aagcttgcgg
      301 acagaataca caatatgcgt acgctcagtt ttagggttcc gcagaagcga cgcgacacag
      361 cgcttgaaac acttgagatt tacgcgccga tagcgcaccg tctcggtatc agaccggcta
      421 aagaagagct tgaggatttg tcgatacgct ttcttgaccc gatagcgtac aaggaaattg
      481 aggaaaattt aaaaaggctc agcaaatcgc gtcttgattt tttggagtcg atggaaggta
      541 agatacgcga gcgaatcacg ggcgttgtaa aagatcccac tattgagggc agaataaaga
      601 gcgtacacgg catttaccgc aaaatgtata tgcaaaacaa aaattttgca gaaatatacg
      661 acgtctacgc catacgtata atagttgaca gcgttataga ttgctataac tgcctcggcg
      721 taattcacga tatgttcaga cccttgccgg gcagatttaa ggactatatc tcgactccga
      781 agccaaatat gtatcagtcg cttcatacaa cggtcatagg caaggagggt ataccgtttg
      841 aggttcagat aagaacctgg gaaatgcacc gcacggccga attcggtatt gcggcgcact
      901 ggaaatacaa actcggagtt cacggaaaat caaaccttga caagcgtctt gcgtggataa
      961 gacagatgct tgaggaccag caggagacag gcgatgtacg cgagatagtt caggccataa
     1021 aaagcgacct tgtccccgaa gatacgtttg ttttcactcc gcgcggcgac gttataagcc
     1081 tccctgccgg tgctaccgtc attgacttcg catatgctat tcatacggcg gtcggtaata
     1141 agatggtcgg tgccaaggta gacggcagaa tagttccgct cgattacgta gtcaaaaccg
     1201 gcgaaatagt cgaggtgctc acgtcctctc aaacgggtaa gggcccgtcg cgtgactggc
     1261 tgagtatagc caagacgagt caggcacgaa acaagatacg ctcatggttt aaaaatgagc
     1321 gacgcgacga aaatatcgcc gagggcaagg cagaggtcga tcgcgaattt aagcgcaact
     1381 tcataaggct cagcgacgaa aactatgaaa aatttgttgc aaatcttgcg gagcggcagc
     1441 atttgagaaa tgttgacgac ttctacgcgg cgataggcta cggcggaata tccgttataa
     1501 ggcttatgcc gtatataaaa gaggagtaca acaaaaatta ccgttctcag gagaccgtgg
     1561 tgctccccga ggacgataag ataaagacaa gccgccgcaa gggcggttcc gacggcgtta
     1621 cggtaagcgg aatagacaac tgccttatca aattttcacg ctgctgcaac ccgcttcccg
     1681 gtgacaatat aataggattt ataacgcgcg gccacggcgt ttcgattcat aagcgtgact
     1741 gtccgaacgt cccgtccgac ttttcaaaat gcgacgagcc gcaaagatgg gtcgccgccg
     1801 cttggaatac tgacgtcaag accgagttca aggcttcgct cataataacc tgcctttcga
     1861 gaatcggtct tatggccgat gtctcggtgc agcttgccgc aatgaaggtc aatatcaacg
     1921 agatatcgtc tcgcgagaca aaagacggca gaagccagat aagtatgacc ataaccgtaa
     1981 gcaatgtcga tcacctgaaa aacgtaatag cgcgtcttaa aaaggtagac ggcgtacttt
     2041 cggttgaaag ataaccgcag tcgaacttat attatgggcg acttcggcaa tatgtccgaa
     2101 ttttaaattt ttacgggaga ttatttctcc ttgacagctg cactttagcg tggtagtata
     2161 ttagcacgct aaagcgattg cacataaaaa gcagaaaggc ggcggattat gaaaagctat
     2221 gaaaaactga ctccggaggg aacaagggat tttctctttg aagagagcga cgcactaagg
     2281 cgtattgaac gcggcctttc cgagctgttt aaagaaaaag gatacaaaaa ggtcataact
     2341 cccaccgtcg aattttttga cgtatttaac cgcgagagcg caggaacgct gccggaaaat
     2401 ctgtacagta tgaccgacgc ttacggcaga cttcttgttt taaggccgga ttccacgctt
     2461 ccgatagcgc gtatagccgc aacaaggctc aagggtgccg agcttcccat aaggctttac
     2521 tacaatcaaa atgtgttttc gcgccgcaaa aagctgaccg gcagaaacga cgaaacctca
     2581 cagagcggca tagagcttat cggggttaac ggcataagag ccgatataga ggttatcgaa
     2641 accgcaatcg aggctctcaa ggtatgcggc gcaccgga
//
LOCUS       DBMDVO010000110         2670 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17936_length_2670_cov_1.129233, whole genome shotgun sequence.
ACCESSION   DBMDVO010000110 DBMDVO010000000
VERSION     DBMDVO010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17936_length_2670_cov_1.129233"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="ACZPE2_02395"
     CDS             complement(<1..1312)
                     /locus_tag="ACZPE2_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318729.1"
                     /translation="MEKQKTSSKRKAAIKVIVAVLLIAIMTGISFAVANFVHNRKSVQ
                     ASSVKNGLSAYELAVQQGYAGSLDDWLVSLQGKSAYQIAVDNGYSGSEKDWSKTLTAM
                     SKKDKSDISSAAFSEKGDLVITLSDGTKINVGKAVGASGKDGKDGKDGIGIKSAKIND
                     KGELVISYSDGNSVNLDKVVGETVNNGVGIASSIINEKGELVLTYTNGETANLGGVVG
                     KDGRDGKNGADGKDGRDGTSGTDGISISAAVINESGELIISYSDGTTATLGKVVGSDG
                     INGVNGTNGKEGISVSSAEINSEGELVLLFSNGQRANVGAVVGAKGDKGDKGEQGLQG
                     AKGDKGDKGENGLNGSDGIGVKKSEINSAGELVITYTNDLTENLGPVVGAKGDKGDKG
                     DQGVQGEKGEKGERGVQGEKGDAGLNGTDGKDGTGINNIEISNDG"
     gene            1441..1647
                     /locus_tag="ACZPE2_02400"
     CDS             1441..1647
                     /locus_tag="ACZPE2_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318730.1"
                     /translation="MDYRTRMRNIREDRDLTQKQVGEIINKSQQGYNHIETGRAELKI
                     EDLIKLCQFYNVSADYFIGLTDQE"
     gene            1845..>2670
                     /locus_tag="ACZPE2_02405"
     CDS             1845..>2670
                     /locus_tag="ACZPE2_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1318731.1"
                     /translation="MPKKTKLKNSNKMDMINGPIWNKLPRFALPVAATAILEQLFNAS
                     DIAVVGNFTGAAKTASVAAVGANSPVIGLILNLFIGIALGTNVIIANAIGRDDRETVQ
                     RAVHTSVLTALIGGFLVMIPGEIFAVQILSAMSIPEDVLPYAVLYLRIYLLGLPVILL
                     YNFLSAVFRSVGDTKTPLIVLALSGCLNVLLNLFFVIVLKMTVNGVAIATVISNAISS
                     IVLFIVLMKSDKYIKLSLRELRIDKRVLGQILEIGLPAGLQSAVFAISNIIIQAAIN"
BASE COUNT          662 a          658 c          546 g          804 t
ORIGIN      
        1 ttccgtcatt tgaaatttca atattattta taccggtgcc gtcttttccg tcggttccgt
       61 tgagacctgc gtcacccttt tcgccttgaa cgcctctttc acctttttcg cctttttcac
      121 cctgtacgcc ttggtcgcct ttgtctccct tgtcaccttt tgcaccgaca acggggccga
      181 ggttttcggt aaggtcgttt gtatatgtga ttacaagctc acctgcgctg ttgatttcgg
      241 actttttaac tcctataccg tccgaaccgt taagaccgtt ttcgcctttg tcacccttat
      301 cgccttttgc tccctgcaag ccttgctcgc ctttatcgcc cttgtcgcct tttgcgccga
      361 caaccgctcc tacattggca cgctgaccgt ttgagaacaa aagcacaagc tcaccctccg
      421 aattgatttc ggcggaagaa acgcttatgc cctctttacc gtttgtgccg ttcacaccgt
      481 ttataccgtc ggaacctact actttaccga gggttgccgt cgtcccgtcc gaatatgaaa
      541 taataagctc acccgactca tttataaccg ctgcggaaat gcttataccg tccgttccgc
      601 tcgtaccgtc tctgccatct tttccgtcag caccgttttt gccgtcacga ccgtctttgc
      661 cgactactcc gccgaggttg gcagtttcgc cgtttgtata agtcaacacc agttcacctt
      721 tttcatttat aattgaagaa gcaataccga ctccgttgtt tactgtttca ccgacaacct
      781 tgtcaagatt tacgctgttg ccgtctgaat aggaaatgac aagctcgccc ttatcattga
      841 tttttgccga ttttattcct atgccgtcct taccgtcttt gccgtctttg ccgcttgcac
      901 cgaccgcctt accgacattg atttttgttc cgtcggaaag cgtaattaca aggtcgcctt
      961 tttccgaaaa tgctgctgag ctgatatcgg atttgtcttt tttcgacatt gcggtaaggg
     1021 ttttgctcca atccttttcg ctgcccgaat atccgttatc gacggcaatc tgatatgccg
     1081 atttaccctg gagagaaaca agccaatcat caagacttcc cgcatatccc tgctgcaccg
     1141 caagctcgta tgcggacaag ccgtttttaa cgcttgatgc ctgcacgctt tttctgttgt
     1201 gcacaaaatt tgcgactgcg aacgatatgc cggtcattat agctatcagc aataccgcca
     1261 caatcacttt gattgccgct ttacgttttg atgatgtttt ctgtttttcc atttatttac
     1321 aactccttct caataataaa atacttcatt tatgataata ctcagttatt gagtatatgt
     1381 caatactcag cttttgagta tgctaacatt ttcacaaatt acaatggagg ttttgtggaa
     1441 atggattaca gaacaaggat gagaaacatc agggaagaca gagatttaac acaaaagcaa
     1501 gtaggtgaaa tcatcaacaa atcccaacag ggctacaacc atatcgaaac cggaagagcg
     1561 gaattgaaaa tcgaggattt aatcaagctc tgtcaatttt ataatgtttc cgccgattat
     1621 tttataggtt taaccgacca agagtaagtt cggttacctc aacgcataat gtagcggtgc
     1681 tttgcggttt ttacactact ttgcgcgatg cggtgctcaa atttatcata tctgtatatt
     1741 gatgttaatt tttttggtga tataattgtg ttgcgtccta caaggcgaca cagctaaaaa
     1801 aacaataccg aaataaaaaa tacgtaaatg caaaaaggat aattatgccg aaaaagacga
     1861 agttaaaaaa cagtaacaaa atggatatga taaacggccc tatatggaac aagcttccgc
     1921 gatttgcgct gcccgtggcc gcaacagcaa tacttgagca gcttttcaac gcctcggata
     1981 tagccgtagt cggaaacttc acgggcgcag ccaaaacggc ctcggttgcc gccgtcggag
     2041 cgaacagccc ggtaatcgga ctgatactca acctgtttat aggtatagcc ctcggtacaa
     2101 atgtaataat agcgaacgca ataggcaggg acgacagaga aaccgtacag cgtgcggttc
     2161 acacctcggt gctcaccgca ttaatcggcg gttttcttgt aatgataccc ggtgaaattt
     2221 ttgccgtgca aattttatcg gctatgagca ttccggagga tgtactgccg tatgccgttc
     2281 tgtatcttcg catatatctg ctcggcttgc ccgtaatact gttgtacaac ttcctgtcgg
     2341 ccgttttcag aagcgtgggc gatacaaaaa cgccgcttat agtgcttgcg ctctccggct
     2401 gcctcaacgt gcttttgaat ttattctttg taatagttct taaaatgacc gtaaacggag
     2461 ttgccattgc caccgttata tccaacgcaa tcagctcgat tgtgctgttt attgttttaa
     2521 tgaagtccga taagtacata aagctgtcgc tcagggagct gcgcatagac aaaagagtac
     2581 tcggtcagat tttggaaata ggtctgccgg caggcttaca gagtgctgtt tttgccattt
     2641 caaacataat aattcaggcg gctatcaaca
//
LOCUS       DBMDVO010000111         2667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17958_length_2667_cov_1.051442, whole genome shotgun sequence.
ACCESSION   DBMDVO010000111 DBMDVO010000000
VERSION     DBMDVO010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17958_length_2667_cov_1.051442"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..1621)
                     /gene="ptsP"
                     /locus_tag="ACZPE2_02410"
     CDS             complement(8..1621)
                     /gene="ptsP"
                     /locus_tag="ACZPE2_02410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1318732.1"
                     /translation="MLKGIGASQGFGIGKAVVIKDANLDYSSVKFTDSDHQKKRLHSA
                     VADFSDETKSLVSELKKAAGKKEAEILEGHLVMLEDPFMLAQMEEAIDNGSVAEAAVD
                     SICTMFINMFSGVDDELTRQRASDVKDIRDSLLRILLGVKSVDISSVPKGSVLIAKDF
                     TPSMTSQINRENVSAIITEVGGVTSHSAILARAMGIPAVLSVVNAVEKISDGDMIIAD
                     GFKGKVFTDPTEEELEEYKTKQAAYLKEKESLNEYFSKETVTKSGVKKHVYGNIGKAE
                     DAQNVLQNGGEGIGLFRTEFLFMDRASLPTEQEQYEAYSTVAKITDGKEVIIRTLDIG
                     GDKAIDYLKIEKEDNPFLGHRAIRYCLDNPKIFKIQLRAILRAAVFGNIKVMLPLVTT
                     LDEVRRAKALIEECKDELKNEGLKFADIPVGVMIETPSAAIISDLLAKEVDFFSIGTN
                     DLTGYTMAVDRGNAAVSKLYDPIQPAVLRLIETTIKNAKKAGIPVGMCGEAAADTRLI
                     PLLCKWGLDEFSVTPSSILHTRKSICECE"
     gene            complement(1654..1920)
                     /locus_tag="ACZPE2_02415"
     CDS             complement(1654..1920)
                     /locus_tag="ACZPE2_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1318733.1"
                     /translation="MVQKTFTITNKMGLHMRPANTFVTEMSKFSSDVDIVFGGKKING
                     KSIMNIMAGCIKCGSEITVECSGADENEMLKKAEELITSGFGEE"
     gene            complement(1989..>2667)
                     /locus_tag="ACZPE2_02420"
     CDS             complement(1989..>2667)
                     /locus_tag="ACZPE2_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIR1318734.1"
                     /translation="LLKKAFSWLPKSMDGIKPVFIYPLFGTLIMGLLMCLVNPVIGVV
                     NSGLSSFLSSLGDTSKILLSIVLAAMMATDMGGPFNKAAYVFGTAAIADGNTWIMAAV
                     MIGGMVPPIAIALSTTFNKKKWTSEEIKSGPVNYLMGLCFITEGAIPYAASDPVRVIP
                     SCLVGSAVAGALTSLFNVTCPAPHGGIFTFIVCDHPFLYIVALIAGSVVGALMLGLLK
                     KDRKESV"
BASE COUNT          632 a          636 c          636 g          763 t
ORIGIN      
        1 ttcgccgtta ttcgcattcg catatacttt ttcttgtgtg caaaatcgaa gagggcgtta
       61 ccgaaaattc gtcaagcccc catttgcaaa gcaatggaat aagacgtgta tcggctgccg
      121 cttctccgca cattccgacg gggatgcctg cctttttggc gtttttaatt gtcgtttcga
      181 taaggcgcag aaccgcaggc tgaatcgggt catacagctt tgaaacagcg gcgtttcctc
      241 tgtcaacagc catggtgtag cccgtcaggt cgtttgtgcc gatagaaaag aaatcaacct
      301 cttttgcaag caaatcggaa attattgccg ccgacggagt ttctatcata acgcccacgg
      361 gaatgtcggc gaatttaaga ccctcatttt taagctcatc tttgcattct tcgataaggg
      421 cttttgctct gcgaacctcg tcaagtgtcg ttacaagcgg aagcataacc tttatgtttc
      481 cgaaaaccgc tgcgcggaga atcgcacgaa gctgaatttt gaaaatttta gggttgtcaa
      541 ggcagtatct tatcgcgcgg tgtccgagaa acgggttgtc ctctttttcg attttaaggt
      601 aatctatcgc tttatctccg cctatatcga gtgtgcgaat gataacctcc ttgccgtccg
      661 ttatctttgc gacggttgaa tatgcttcgt actgctcctg ctccgtggga agagaggctc
      721 tgtccataaa cagaaattcc gttctgaaga ggcctatgcc ctcgccgccg ttttgcaata
      781 cattctgtgc gtcctcagcc ttgccgatgt tgccgtacac atgctttttt acgccggatt
      841 ttgtaacggt ttcttttgag aagtattcgt tgaggctttc tttttccttc agatatgccg
      901 cctgcttggt tttgtattct tcaagctctt cctctgtggg gtcggtaaat acttttccct
      961 tgaagccgtc tgcaataatc atatcgccgt ccgagatttt ttcaaccgca ttgacgaccg
     1021 acaaaacggc gggtatgccc atggcacggg cgagtattgc gctgtgactc gtaacgccgc
     1081 cgacctcggt gataattgcg gaaacatttt ccctgtttat ttgtgacgtc atggacgggg
     1141 tgaaatcctt ggcgatgaga acagagccct tcggtacgga tgaaatatcc acacttttta
     1201 cacccagaag aatgcgcaaa aggctgtctc ttatatcctt tacatccgag gcgcgttggc
     1261 gagtaagctc gtcgtcaacg ccggagaaca tattgataaa cattgtgcat atgctgtcaa
     1321 ccgccgcttc ggcaacagag ccgttgtcga ttgcttcttc catctgtgcg agcataaacg
     1381 ggtcctcaag cataacaaga tgtccctcga gtatctccgc ctctttcttt ccggcagcct
     1441 ttttaagctc cgacacaagt gattttgttt cgtcggaaaa gtcggcgact gccgagtgca
     1501 gacgcttttt ttgatgatcc gaatcggtaa attttacgga tgaatagtca aggtttgcgt
     1561 cctttataac aacggccttg ccgattccga agccttgaga tgcgccgatt cctttaagca
     1621 ttaaacccac cctttcttta agccgcggcg atattattct tcgccgaagc ccgatgtgat
     1681 aagctcttcg gcttttttga gcatttcgtt ttcgtctgcg ccgctgcact ctacggtgat
     1741 ttcgcttccg catttgatgc agcctgccat tatattcata atgctcttgc cgttgatttt
     1801 tttgccgccg aaaacaatgt caacgtccga agagaattta gacatttccg ttacaaaggt
     1861 gtttgccgga cgcatatgaa gtcccatttt attagtgatt gtaaaagttt tctgtaccat
     1921 aataattctc cttatgttta atacctgccg agaggatgat gcccctcggc acggtttttg
     1981 attgtttgtt atacggattc tttacgatcc tttttaagaa gaccgagcat cagtgctccg
     2041 acaaccgagc ctgcaataag ggctacgata tagagaaacg gatggtcgca tactataaat
     2101 gtgaagattc cgccgtgagg tgcggggcat gtaacgttga acagtgatgt aagagcacct
     2161 gcgacagcag agcctactaa gcatgagggg ataactctga ccgggtcgga agcggcgtag
     2221 gggatagcac cctccgtaat gaagcacaga cccataagat agtttacggg gcctgatttg
     2281 atttcttccg atgtccactt cttcttgttg aaggttgtcg aaagtgcgat tgcaatcggg
     2341 ggaaccatac cgcctatcat aacagccgcc ataatccatg tgttgccgtc ggctattgca
     2401 gccgtgccga aaacataagc cgccttgttg aacgggccgc ccatatccgt tgccatcatt
     2461 gccgcaagta ctatggaaag gagtattttg cttgtatcgc cgagcgacga gaggaacgag
     2521 gaaagaccgg agttgacaac gcctataacg gggtttacaa gacacataag aaggcccatg
     2581 ataagtgtgc cgaaaagagg atagataaat acgggcttta ttccgtccat tgatttcgga
     2641 agccaagaaa atgccttttt gaggaga
//
LOCUS       DBMDVO010000112         2664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_17994_length_2664_cov_1.271557, whole genome shotgun sequence.
ACCESSION   DBMDVO010000112 DBMDVO010000000
VERSION     DBMDVO010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17994_length_2664_cov_1.271557"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /gene="dxs"
                     /locus_tag="ACZPE2_02425"
     CDS             complement(<1..1256)
                     /gene="dxs"
                     /locus_tag="ACZPE2_02425"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1318735.1"
                     /translation="MKYLDKIKSPADVKNLNEDDLPLLAQEIREVLVDTVSQNGGHLA
                     SNLGVVELTIALHRVFDSPKDKIIWDVGHQAYTHKLLTGRYPEFETIRREGGLSGFTN
                     PSESEHDIFYSGHSSTSISEALGVATANGLNNDKHTAIAVIGDGALTGGLAYEALNNA
                     GRSKQRLIVILNDNEMSISKNVGSVARYLAVLRSKPGYFRLKARTEKIINRIPLIGKK
                     ISEQLFKSKTRLKNLIYKSTFFEDLGFRYMGPIDGHNITQLCEALEGAKMVNAPVLLH
                     INTVKGKGYDFAEKSPSEFHGISKFNINTGEPVYSGTNFSAEFGDFLCDIASKDKRIC
                     AITAAMSLGTGLEKFRKQFPDRFYDVGIAEEHAVTFSSGLARGGMVPVFAVYSTFLQR
                     CYDQLVHDGAMQHLHMIIAVDRAGFVG"
     gene            complement(1282..2160)
                     /locus_tag="ACZPE2_02430"
     CDS             complement(1282..2160)
                     /locus_tag="ACZPE2_02430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1318736.1"
                     /translation="MEYTHEKYIEMIDEALKVYLGSVSDGQDVVLDAMKYSAEGGGKR
                     IRPVLTLEFCRVCGGDVQRAIPFACAVEMIHTYSLIHDDLPCMDNDDMRRGKPSCHKA
                     FGEEYALLAGDGLLTLAFETLSKAEDIKVDTFKEALFTLSNLAGINGMVGGQVLDLKA
                     EKSKPTLSALQTIDRLKTGALIKAACTLGCLASEKYDGQKIAAASVYAENIGLAFQIV
                     DDILDVTSDTATLGKPVGSDDKNEKITYVKLMGIEKSQETVDELTKEAVNALGVFDGD
                     TSFLEKLAYKLALRKK"
     gene            complement(2166..2357)
                     /gene="xseB"
                     /locus_tag="ACZPE2_02435"
     CDS             complement(2166..2357)
                     /gene="xseB"
                     /locus_tag="ACZPE2_02435"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1318737.1"
                     /translation="MKQTFESAVTRIEEISKLLEDKNISLDDSIKLFEEGTRLSDYCS
                     ECLKNAEQKITELKNKEAE"
     gene            complement(2354..>2664)
                     /locus_tag="ACZPE2_02440"
     CDS             complement(2354..>2664)
                     /locus_tag="ACZPE2_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014640.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1318738.1"
                     /translation="SKGALKSPDEMLSNRSQRLDTAFSKMLSSYENRIGGKKVEFISA
                     ATALSKLDPMSVLMRGFAFVSDKNGKNVYSSQALAKGDKINVRFHDGSAVCEVKEITQ
                     "
BASE COUNT          675 a          667 c          564 g          758 t
ORIGIN      
        1 ccgacaaagc ccgcgcggtc aacagcgatt atcatatgaa gatgctgcat agctccgtca
       61 tgtacaagct ggtcatagca cctttgaagg aacgtagaat atacggcgaa aaccggcacc
      121 ataccgcctc ttgcaagtcc cgaggaaaag gttacggcgt gttcttcggc tatgccgaca
      181 tcataaaatc tgtcgggaaa ctgctttctg aatttttcaa gacccgtgcc gagagacata
      241 gccgcggtta tagcgcatat acgcttatcc ttagaggcta tatcgcacag gaaatcaccg
      301 aactcggcgg aaaaattcgt gccgctgtat acgggctcgc ccgtatttat attgaatttt
      361 gaaatcccgt ggaactcgct cggggatttc tcggcaaagt cataaccctt acccttcacc
      421 gtgttgatat gaagaagcac cggggcattt accattttcg caccctcaag agcctcgcaa
      481 agctgagtta tattgtgtcc gtcaataggt cccatataac ggaagccgag gtcctcaaaa
      541 aaggtgcttt tatagattaa attctttagc cttgtctttg atttaaaaag ctgctcggat
      601 atttttttgc ctatgagcgg tatgcgattg attatttttt cggtacgcgc cttcaagcgg
      661 aaataacccg gttttgaacg aagcacggca agatacctgg cgacagagcc gacgtttttt
      721 gaaatagaca tctcgttatc gttaagtatg actatgagtc gctgcttgga tctgcctgca
      781 ttgttgagtg cctcataagc gagtccgccc gtcagcgcac cgtcgcctat tacggctatt
      841 gcggtatgct tatcgttatt aagaccgttt gcggttgcaa caccgagagc ctctgaaata
      901 gaagtactcg agtgtccgct gtaaaaaata tcatgctcgc tctccgacgg atttgtaaag
      961 cccgaaagtc cgccctcacg gcgtattgtc tcaaattcgg gatacctgcc cgtcaaaagc
     1021 ttatgcgtat atgcctgatg gccgacatcc caaataattt tatccttggg cgaatcaaaa
     1081 accctgtgaa gagcaattgt aagctcgaca acaccgagat tcgacgccaa atgaccgccg
     1141 ttttgcgaaa ccgtatctac gagaacctct ctgatttcct gcgccaaaag cggcaaatca
     1201 tcttcattga gattttttac gtccgcaggc gattttattt tatcaagata cttcattaaa
     1261 aaaacttctc ccgaagaccg gttatttttt tcttaaagcc agcttatatg ctaatttttc
     1321 aaggaaagac gtgtcaccgt caaaaactcc gagagcgttt accgcctctt tagtcaattc
     1381 gtcaaccgtc tcctgtgatt tctcaatacc cataagttta acatacgtta tcttttcatt
     1441 tttgtcgtcg ctgccgacgg gtttgccgag tgtggcggta tcgcttgtga cgtcaagtat
     1501 atcgtctaca atctgaaaag caagccctat gttttcggca taaacagacg ccgcggctat
     1561 cttttgccca tcatattttt ccgatgcgag acagcctaag gtacacgccg cctttataag
     1621 tgcaccggtt ttaagacggt ctatcgtttg aagagctgag agcgttggtt tgcttttctc
     1681 cgctttgagg tcaagcacct gaccgccgac cataccgttg atacctgcaa gatttgagag
     1741 tgtaaacagt gcctctttaa aagtatcgac tttaatgtct tcggcttttg agagcgtttc
     1801 aaacgcaaga gtcaaaagcc cgtcgcctgc caaaagcgca tattcctcgc cgaatgcctt
     1861 gtggcacgac ggcttgccgc gcctcatatc atcgttatcc atacacggaa gatcgtcgtg
     1921 aatcagcgaa taagtgtgaa tcatctctac ggcgcacgca aacggtatag ccctttgcac
     1981 atctccgccg cacacacggc aaaactcaag cgtcaatacg ggtcttattc tttttcctcc
     2041 gccctctgca ctgtatttca tcgcgtcaag aacaacatct tgaccgtccg aaaccgagcc
     2101 gaggtacact ttcaacgcct cgtctatcat ctcaatatat ttttcgtgag tatattccat
     2161 aaatatcact ccgcttcttt gtttttgagc tccgttattt tctgctctgc attttttaga
     2221 cattcgctgc aataatccga aaggcgcgtg ccctcttcaa aaagcttgat tgaatcatca
     2281 agtgagatgt tcttgtcttc aagcagcttt gatatttctt caatccgcgt tacggcggac
     2341 tcaaaggtct gtttcattgc gttatctcct taacctcaca aacggcagag ccatcgtgaa
     2401 atctgacatt gattttatca cccttcgcaa gtgcctgcga cgagtataca ttttttccgt
     2461 ttttgtcgga aacaaaggca aatccgcgca taagcaccga catagggtca agctttgaca
     2521 gcgcagttgc ggcagatata aattccactt ttttgccgcc gatacggttt tcatatgatg
     2581 acagcatttt tgaaaaagcc gtatcaaggc gttgactccg attcgacagc atttcgtcgg
     2641 gagattttaa agctccctta gacg
//
LOCUS       DBMDVO010000113         2657 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18066_length_2657_cov_1.359937, whole genome shotgun sequence.
ACCESSION   DBMDVO010000113 DBMDVO010000000
VERSION     DBMDVO010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18066_length_2657_cov_1.359937"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="ACZPE2_02445"
     CDS             complement(<1..758)
                     /locus_tag="ACZPE2_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1318739.1"
                     /translation="MKKKIFSAICLLLSAVCIFSACRKDNGNGGSDEQTGSAASGDST
                     ASSANDLILPYSNNDSLNPFFAVGTENISLTDLAYDPLFKVKADYTPENVIAEKGDIS
                     GTSVTVTLSGARFSDGSSVSPQDVVYSFNKAKASSRYGQQLSNIQSAKASGGAVTFTL
                     SSPDPYVLNALSFPVVKNGTADKTENIPIGSGPYVYRDGVFSKNENYGGSVNIPEIKL
                     YEIENFTYAENALEIGNVNFLFKDMSDSVYRHVSV"
     gene            complement(774..1544)
                     /locus_tag="ACZPE2_02450"
     CDS             complement(774..1544)
                     /locus_tag="ACZPE2_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR1318740.1"
                     /translation="MNILAVGDVVSASGCEKLRNCLPKFKRENNIDVTVVNGENSAVG
                     NGILPMSADDIFTSGADVVTGGNHTFRRREIYDELERNEFLLRPANYGKEAPGRGYTI
                     VDRGAYRVGVVNLCGTVFMEPLENPFICIDRVLEKLKRETDIIIVDFHAEATAEKRAM
                     GFYLDGRASAVFGTHTHVPTADEQILPHGTGYITDVGMTGPKMSVLGVTPELAIEKMK
                     TNLPVRFQNPDGEAELDGVIFTVSRDTAKTVRIQRISL"
     gene            complement(1643..>2657)
                     /gene="rny"
                     /locus_tag="ACZPE2_02455"
     CDS             complement(1643..>2657)
                     /gene="rny"
                     /locus_tag="ACZPE2_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1318741.1"
                     /translation="DEQDALAREIISTAIQRCAADQAAEATVSVVTLPNDEMKGRIIG
                     REGRNIRTLETITGVDLIIDDTPEAITVSSFEPVRREIARLTIEKLISDGRIHPARIE
                     EMYEKSKREVEQTIRQTGERAVVDAGVNGIHPELVKILGKLKYRTSYGQNVLNHSLEV
                     SYIAGLMAAELGADVKTAKRAGLLHDIGKALDREFEGSHVDLGVEYARKYKENENVVH
                     AIQAHHGDVEAKTVVACLVQAADAISAARPGARRENVQNYIKRLEKLEEIATSFEGVE
                     KSFAIQAGREIRIMVKPDVISDDQMALVAREVVKKIEDELEYPGQIKVNIIRENRTVD
                     YAK"
BASE COUNT          620 a          697 c          590 g          750 t
ORIGIN      
        1 acggagacat ggcggtaaac gctgtcgctc atatccttga agagaaagtt cacattgcct
       61 atttcgagag cattttcggc ataggtgaaa ttttctattt cataaagctt tatctcgggt
      121 atatttacgc tgccgccgta attttcattc ttggaaaaca ccccgtccct gtacacataa
      181 gggccgctgc ctatcggtat attttccgtc ttatcggctg tgccgttttt tacaacggga
      241 aaactgagtg cattcagcac atacgggtcc ggtgaagaga gcgtaaacgt aacagctccg
      301 ccggacgcct ttgccgactg aatattagac agctgctgcc cgtagcgcga cgacgccttt
      361 gccttattga acgaatatac gacgtcctgc ggcgaaacgg acgaaccgtc cgaaaagcgc
      421 gcacctgaga gtgtaacggt aaccgaagtt ccgctaatat cacccttttc ggctattaca
      481 ttttcgggcg tatagtctgc cttgacctta aaaagcggat cgtaggctaa atccgtaagg
      541 cttatatttt ccgtgccgac ggcaaaaaaa ggattcagcg aatcattatt ggaatacggc
      601 aaaataaggt catttgccga tgaggcggtg ctgtcgcccg aggcggcaga gccggtctgc
      661 tcatccgagc cgccgttgcc gttatccttg cggcaggccg aaaaaataca taccgcagaa
      721 agcaaaaggc atattgcaga aaatattttc tttttcactg tcgcactctc cttttaaagc
      781 gatattctct gaatccgaac ggtttttgcc gtgtcacggc ttacggtgaa tataacaccg
      841 tcaagctcgg cttcaccgtc gggattttga aagcgcaccg gcaaattggt tttcatcttt
      901 tctatcgcaa gctcgggggt aacgcccaat acggacattt taggcccggt catgcctaca
      961 tcggttatat atcccgtacc gtgcggcaat atttgctcgt ctgccgtggg cacatgggtg
     1021 tgagttccaa acacagccga agcccttccg tcaagataaa agcccatagc gcgcttttcg
     1081 gcagtcgcct ctgcatgaaa atcgacaatt attatatcgg tttctctttt cagcttctcc
     1141 aatacacgat ctatacatat aaagggattt tcgagcggct ccataaacac cgtgccgcag
     1201 agatttacca cgcctaccct gtatgcgccc ctgtcaacta tcgtatagcc tctgccggga
     1261 gcctcttttc cgtagtttgc gggtcttaaa agaaactcgt ttctctcaag ctcgtcatat
     1321 atttcacgac gtctgaaggt gtggttgccg ccggttacaa catccgcgcc gctcgtaaaa
     1381 atatcatcgg cggacatcgg caatatgccg tttccgacag cggaattttc gccgtttacc
     1441 accgtcacgt ctatattatt ttctctttta aatttcggta aacaattcct cagcttttcg
     1501 cagcccgacg ccgatacgac atcgcccacg gcgaggatgt ttattgtatt tgacataatg
     1561 caccgtttct gtttgttttt tcgaaaagct aagccccgat aacactcaaa gtttttctct
     1621 ttaaggcatc agggcttaaa aattatttcg cgtaatcgac agtcctgttt tcgcgtatga
     1681 tgttgacctt tatctgaccg ggatattcaa gctcatcctc aatctttttg acaacttcgc
     1741 gcgcaacaag tgccatctga tcgtcactta taacatcggg cttaaccatt atgcgaatct
     1801 cacggcctgc ctgaattgca aatgacttct caacgccctc aaacgacgtt gctatttctt
     1861 cgagtttttc aagacgcttg atgtagttct gaacattttc acgtcttgcg ccgggtcttg
     1921 cggctgaaat tgcgtccgcc gcctgaacga gacaagcaac aaccgtcttt gcctccacgt
     1981 cgccgtggtg agcctgaatt gcatgtacta cattctcgtt ttccttatat ttccttgcgt
     2041 attcaacgcc caaatcgaca tgagagcctt caaattcacg gtcaagcgcc ttgcctatat
     2101 cgtgaagaag tcccgctctt ttagccgttt taacatcggc accgagctct gccgccataa
     2161 gccccgctat gtaggaaacc tcaagcgaat gattaagcac attctggccg tagcttgtgc
     2221 ggtattttaa ttttccgagt atcttgacga gctccggatg tatgccgttt acaccagcgt
     2281 caactactgc gcgttcaccg gtctgacgta tggtctgctc aacctcgcgc tttgattttt
     2341 cgtacatctc ctcaatgcgt gcgggatgta ttctgccgtc ggaaataagc ttttcgatag
     2401 taagtcttgc tatttcgcga cgaaccggtt caaaacttga aacggttatg gcttccggtg
     2461 tgtcgtcgat tataaggtca acaccggtta ttgtttcaag tgtgcgtatg tttctgccct
     2521 cgcggccgat tatacggccc ttcatctcgt cattgggaag agtaaccacc gaaaccgtgg
     2581 cttctgcagc ctgatcagcg gcacagcgct gtatagcggt agagattatc tcacgcgcaa
     2641 gagcgtcctg ctcatcc
//
LOCUS       DBMDVO010000114         2656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18075_length_2656_cov_1.281800, whole genome shotgun sequence.
ACCESSION   DBMDVO010000114 DBMDVO010000000
VERSION     DBMDVO010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18075_length_2656_cov_1.281800"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="ACZPE2_02460"
     CDS             <1..213
                     /locus_tag="ACZPE2_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1318742.1"
                     /translation="DSKNIKDARDAGCSGMDFSAEFNACRPEWIRKVHDNGLKTACWT
                     VDNREMLASMIDAGVEYITTNSILPE"
     gene            238..1029
                     /locus_tag="ACZPE2_02465"
     CDS             238..1029
                     /locus_tag="ACZPE2_02465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1318743.1"
                     /translation="MARFCPLFSGSSGNSIYIGNNDGGILVDTGMSAKKIGEALFNIG
                     VAPESLNAVFVTHEHSDHIKGLRVFASKYHIKVYGTKGTLSALDDMGILNGKFETEEV
                     SFSGNEAGPLFVTPFHTSHDARESCGYKIDCSDGRKISIATDLGEMTEEVFSAVSGSD
                     LVMLESNHDVMMLENGPYPYSLKKRILGKKGHLSNDACASTAERLVSGGTTRIYLGHL
                     SRENNMPALAYQTTCSALTMAGAKENSDYILKVAKPVCDEKAMIL"
     gene            1026..1508
                     /gene="rlmH"
                     /locus_tag="ACZPE2_02470"
     CDS             1026..1508
                     /gene="rlmH"
                     /locus_tag="ACZPE2_02470"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1318744.1"
                     /translation="MISVTVIALGKLKESYMRDFCAEYEKRLKAFCKINITELSPVRL
                     SQNPSEGEIKKALDSEAALIKSKIPSGAYVFSMCIEGRQMSSVDFSEKISATALSGKS
                     NIAFIIGSSFGLSDEIKKMSDFKFSMSEMTFPHQLARCMLLEQIYRAFEISSGGKYHK
                     "
     gene            1536..2189
                     /locus_tag="ACZPE2_02475"
     CDS             1536..2189
                     /locus_tag="ACZPE2_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR1318745.1"
                     /translation="MFCTNCGAALPNGAEICPNCNRPVSRSSFAQGEAPVSNVNYNGQ
                     NAPYKPESCNQNYNSPSGNTNGFYGSQQNNPVNSTYNAPVNGAAYQQNRPEQYNQYSQ
                     NGGAYNPQTYRNPNEKANVGLCVASVFFPIVGLILFLVQKNDTPRAAKSYLLCAAISF
                     GVQVLLSIIVMVIMWTAVWAAVPYIDDSFSYDYDYDYDTATNLFTALISPVRLFFMK"
     gene            complement(2274..>2656)
                     /locus_tag="ACZPE2_02480"
     CDS             complement(2274..>2656)
                     /locus_tag="ACZPE2_02480"
                     /inference="COORDINATES: protein motif:HMM:NF027645.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR1318746.1"
                     /translation="VEITLSKGGFIFRSQERSQDLEDALDKCVDSLIRQIRKNKTRLA
                     KRIRDVSFDEAFNSADDSAEEEYDIVRTKAVILKPQSSEEAILQMNMLGHEFYMFRNA
                     DTDDVNVVYKRADGGYGILEPTEG"
BASE COUNT          769 a          644 c          610 g          633 t
ORIGIN      
        1 gacagcaaaa acattaaaga tgcgagagac gcaggctgca gcggtatgga cttctcggcg
       61 gaatttaacg cctgccgtcc ggagtggata cgcaaggttc atgacaacgg tcttaaaacc
      121 gcctgctgga cggttgacaa ccgagaaatg ctcgcctcaa tgatagacgc cggcgtagag
      181 tacatcacga caaattccat tcttccggaa taacagacac aaaagcaagg agactttatg
      241 gcaagatttt gtccgctttt ttccggaagc tccggcaact caatatacat aggcaataac
      301 gacggcggaa tactcgtaga cacgggaatg agcgcaaaaa aaatcggcga agcactcttt
      361 aacataggcg tcgccccgga gagtttaaac gccgtttttg tcacacacga acactccgac
      421 catataaagg gcttgcgcgt attcgcttca aaatatcaca taaaggtata cggaacaaag
      481 ggtacgctct cggcacttga cgatatgggc atattaaacg gcaaatttga gacggaggag
      541 gtctcgtttt ccggtaacga agcaggtcct ctttttgtga ccccgtttca cacctcgcat
      601 gacgcaagag aaagctgcgg ctacaaaata gactgcagcg acggcaggaa aatctccata
      661 gccaccgacc tcggtgaaat gaccgaagag gttttttcgg cagtaagcgg ctcagacctt
      721 gttatgcttg agtcaaacca cgacgttatg atgctcgaaa acggccctta cccgtattct
      781 ttgaaaaaga gaattctcgg caaaaaaggg cacctttcca acgacgcgtg tgcaagcacc
      841 gccgaaaggc tcgtaagcgg cggtaccacg agaatttatc tcggccactt aagccgtgag
      901 aacaatatgc cggctctcgc atatcaaacc acgtgctcgg cacttaccat ggcgggagca
      961 aaagagaaca gcgactatat actcaaggtt gcgaagcccg tctgcgacga gaaagcgatg
     1021 atattatgat aagcgttacc gttatagcac tcggcaagct caaggaaagc tatatgcgcg
     1081 atttttgcgc agagtacgaa aaacggctca aggcgttttg caaaataaac ataacagagc
     1141 taagtcccgt acgtctttca caaaatccga gcgagggtga aataaaaaag gctctcgaca
     1201 gcgaggctgc gcttataaaa tccaaaatac cgtccggtgc ttatgtattt tcgatgtgca
     1261 ttgaaggcag gcagatgtca agcgttgact tttccgaaaa aatatccgcc actgcgctgt
     1321 ccggcaaaag caatatagcc tttattatcg gcagctcctt cggactcagt gacgaaatca
     1381 aaaaaatgtc ggattttaaa ttttcaatgt cggaaatgac ctttccgcat cagctcgcgc
     1441 gttgtatgct ccttgagcag atatacagag cattcgaaat atcttccggc ggaaaatacc
     1501 ataaataaaa aattcaaaag gattgggtga ttattatgtt ttgtacaaat tgcggtgcgg
     1561 ctttaccgaa cggtgcggaa atatgcccga attgcaaccg acccgtcagc cggtcaagct
     1621 tcgcacaagg agaagcccct gtatcaaatg tgaattacaa cgggcaaaac gctccgtaca
     1681 aaccggagag ctgtaatcag aattacaaca gcccctcggg aaatacaaac ggcttttacg
     1741 gctctcaaca aaacaatccc gtaaacagca catataatgc tccggtaaac ggagccgctt
     1801 atcagcaaaa ccgaccggag caatacaatc agtacagtca aaatggcgga gcctataatc
     1861 cgcagacata tcgtaatccc aacgaaaagg caaacgtggg gctgtgcgtt gcctcggtgt
     1921 tttttccgat tgtcggtctt atactttttt tggtgcagaa aaacgatact ccgcgcgccg
     1981 caaaatcata tcttctttgt gccgcaataa gcttcggcgt gcaggtgctt ttaagtataa
     2041 ttgttatggt gattatgtgg acagcggtat gggcggctgt gccgtacatc gacgacagct
     2101 tttcttatga ctacgactat gactatgaca ctgcaacaaa tctgtttacg gcattgattt
     2161 cgcccgtaag actcttcttt atgaaatgat atagttcatc cgaaatgcca agcaccgccc
     2221 ggccaaagac cgagcggtgc tgtttatttt attatattat gccgccgagc ttatcagccc
     2281 tcggtcggct caagtatacc gtagccgccg tcagcacgct tataaaccac atttacgtcg
     2341 tccgtgtcgg cattgcggaa catataaaac tcgtgaccga gcatattcat ctgaagtatg
     2401 gcctcctcgg aagattgcgg ttttaaaatc acagccttcg ttctgactat gtcatactcc
     2461 tcctcagcgg agtcgtcagc cgaattaaac gcctcgtcaa aggatacatc acgtatacgc
     2521 tttgcaagtc ttgtcttgtt ttttcttatc tgacgaatga gtgaatcgac acatttatca
     2581 agcgcgtcct caaggtcctg tgagcgctcc tgacttctga atataaaacc gccctttgag
     2641 agcgttatct caacaa
//
LOCUS       DBMDVO010000115         2639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18239_length_2639_cov_1.549645, whole genome shotgun sequence.
ACCESSION   DBMDVO010000115 DBMDVO010000000
VERSION     DBMDVO010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_2639_cov_1.549645"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="ACZPE2_02485"
     CDS             complement(<1..1110)
                     /locus_tag="ACZPE2_02485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1318747.1"
                     /translation="METCYDLVVVGGGPAGMAAALEAYDKGVKKILILERADTLGGIL
                     EQCIHTGFGLHYFGEELSGPEYAGRFIKQVKETDIDVKVDTMVLDISDDNVVTAVNNK
                     DGLLTIKAKAVVLAMGCRERPRGALSIAGCRASGIMTAGTAQKYVNIDGYMPGKTVVI
                     LGSGDIGLIMARRMTLEGAKVKAVCELMPYSGGLTRNIVQCLEDFGIPLRLSCTVVST
                     HGKDRLEGVTVANVDEHLKPIPGTEEYIPCDTLMLSVGLIPENELSKNAGIELDRITN
                     GPIVDEYRETKHKGVFACGNVLQVHDLVDYVTEESQLAGAGAAKYIRGEKNCDEFVNT
                     KGENGVRYIVPQRISHDTDKDVKLYFRVGRVLKNVT"
     gene            complement(1120..2550)
                     /locus_tag="ACZPE2_02490"
     CDS             complement(1120..2550)
                     /locus_tag="ACZPE2_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1318748.1"
                     /translation="MKYDVAVIGGGVVGALISRELSKYDVKTAVLERCNDIAMGTTKA
                     NSAIVHGGFDAVSGTVKAKLNVEGTAIMPKLCKELNVPYRNNGSLVVAFSEKEMEHVH
                     LLYDRGIKNGVPELEIIDSKRLHELEPNISDEAVGALYSKSAGIVCPYELTIAAAENA
                     CENGVEFIRNCLVKAISFNGSEFVLDTTKGDITASYVINAAGTHSDEVARMIGDDSIK
                     IVARKGEYYLLDKSFGHLVDRTVFQCPNEMGKGVLVTPTVDGNLLVGPSATDEDNKDD
                     VDTTPEGLEFILSKALKSVPNVNVRGAITSFAGVRAHPVTDDFIIGYSDKNDRFINVA
                     GIESPGLSAAPAIAKYVAELFAEKAAPQKKADFNGSRPAPVRFRNMTKEEREKLIAKD
                     KRYGRIICRCETITEGEILDAIHAPVGARDVDGVKRRTRAGMGRCQGGFCGSKVVEIL
                     SKELGVPMNEITKFGGESKIIFDRTK"
BASE COUNT          630 a          718 c          573 g          718 t
ORIGIN      
        1 agttacgttt ttaaggactc tgccaacacg gaaatagagc ttaacgtcct tatcggtatc
       61 gtgggaaatt ctctgcggaa ctatgtatct tacgccgttt tcgccctttg tatttacaaa
      121 ttcgtcgcag ttcttttcac cgcggatata ttttgcggca cctgcgcctg caagctggct
      181 ctcctctgtt acatagtcaa caagatcgtg cacctgtaaa acgtttccgc acgcaaacac
      241 gcccttgtgc tttgtctcac ggtattcgtc aactatcggg ccgtttgtta tacggtcaag
      301 ctcgataccg gcattctttg aaagctcatt ttcgggaatg agacctaccg acagcataag
      361 cgtgtcacag ggtatatact cctccgtgcc gggaatgggc ttcagatgct cgtctacatt
      421 ggcaacggta acaccctcaa gacggtcctt gccgtgagtg ctgacaaccg tgcaggaaag
      481 gcgaagcgga ataccgaagt cctcaaggca ctgaacgata tttctcgtaa gtccgcccga
      541 atacggcata agctcgcaaa cagccttaac cttcgcgccc tctaaggtca tacgtcttgc
      601 cataataaga cctatatcgc ccgagcctaa gattacaaca gttttaccgg gcatatatcc
      661 gtcaatatta acgtatttct gagcggttcc ggcggtcata atacccgacg cacggcagcc
      721 tgctatggaa agcgcacctc tcggtctttc acggcagccc attgcaagca caacagcctt
      781 cgcctttatt gtgagaagcc cgtccttatt gttaacggca gttaccacgt tgtcgtctga
      841 aatatcaaga accattgtat cgactttaac gtcaatgtcg gtttctttaa cctgctttat
      901 gaatctgccg gcatactcgg gaccggagag ctcctcgccg aagtaatgca gaccgaagcc
      961 ggtgtgtata cactgctcaa gtataccgcc gagagtatcg gcacgttcca aaataaggat
     1021 ttttttaact cccttgtcat aggcctcaag cgcagccgcc attccggcag ggccgccgcc
     1081 tacaactact aaatcataac acgtttccat tattccacct catttcgtcc tgtcaaagat
     1141 aatctttgac tcgccgccga attttgttat ctcgttcatc ggaacgccga gttccttcga
     1201 aagtatctcg actacctttg agccgcagaa gccgccttgg cacctgccca tacctgctct
     1261 tgtgcgacgc ttaaccccgt caacgtctct tgcgcctacg ggagcgtgta tggcgtcgag
     1321 aatttcgccc tcggtaatgg tctcgcagcg acagataatc ctgccgtaac gtttatcctt
     1381 tgcaataagc ttttcacgct cttcctttgt catattgcgg aaacgaacgg gtgccggacg
     1441 actgccgtta aagtctgctt tcttttgggg agcggccttt tcggcgaaaa gctccgcgac
     1501 atattttgca atggcgggcg cagcggaaag acccggcgat tctatacccg caacattgat
     1561 aaagcggtcg tttttatcgg aatagcctat gataaagtca tccgtaacag gatgcgctct
     1621 gacacctgca aacgatgtta ttgcgcctct gacatttacg ttcggaacgg atttcaaagc
     1681 cttagagaga ataaactcaa ggccctcggg agttgtatcc acatcgtcct tattatcctc
     1741 atccgtcgcc gacgggccga caagaaggtt gccgtcaacc gtaggagtta ccaatacgcc
     1801 cttgcccatt tcgttggggc actggaatac ggtgcggtca acaagatggc cgaaggattt
     1861 atcgagaaga taatactcgc ctttacgggc tactatcttt atggaatcgt cgcctatcat
     1921 acgggcaacc tcgtcgctgt gagtacctgc ggcatttatt acatatgacg cggttatatc
     1981 gcccttcgtg gtatcgagaa caaattccga gccgttaaag cttattgcct ttacaagaca
     2041 atttcttata aattcaacgc cgttttcgca agcattctca gccgccgcta ttgttaactc
     2101 atacgggcat actatacccg cggattttga gtaaagtgcg ccgactgcct cgtcgctgat
     2161 attcggctca agctcatgga gcctctttga atcaattatt tctagctcgg gaacgccgtt
     2221 tttaattccg cggtcataca gaagatgcac atgctccatt tccttttcgg aaaaagcgac
     2281 taccaaagag ccgttatttc tgtacggaac attcagctct ttacaaagtt tcggcataat
     2341 agccgtaccc tcaacattca gctttgcttt cacggtaccc gacacagcat caaatccgcc
     2401 gtggacgatt gcgctgtttg ccttggtagt tcccattgca atgtcgttac acctttcaag
     2461 cactgctgtc tttacgtcat atttcgagag ttctctcgaa ataagagcgc cgacaacgcc
     2521 gccgcctata acggcaacat cgtacttcat aaaaaatcca ccccttgttt ttattcagct
     2581 tagtgagact tcactaatct atatcctggc cggcaaaatc gtccttatcc tgcttgctg
//
LOCUS       DBMDVO010000116         2636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18278_length_2636_cov_0.942406, whole genome shotgun sequence.
ACCESSION   DBMDVO010000116 DBMDVO010000000
VERSION     DBMDVO010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18278_length_2636_cov_0.942406"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(19..504)
                     /gene="coaD"
                     /locus_tag="ACZPE2_02495"
     CDS             complement(19..504)
                     /gene="coaD"
                     /locus_tag="ACZPE2_02495"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1318749.1"
                     /translation="MKTVICPGSFDPVTMGHLDIIRRSAKMFDKVIVVVMTNYHKKNN
                     YAFTSEERVELLKRCTADMPNVDVDRYDGLLADYARRKEAVAIVKGLRAVSDFEYEFQ
                     QALTNKKLNNELETVFITTRAENMYLSSSVVKQVCELGGDISTFVPAEIREDIIKKIK
                     K"
     gene            complement(520..1056)
                     /gene="rsmD"
                     /locus_tag="ACZPE2_02500"
     CDS             complement(520..1056)
                     /gene="rsmD"
                     /locus_tag="ACZPE2_02500"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1318750.1"
                     /translation="MRVITGIARGRKLVTLEGEEVVRPTTDRVKEAMFSIIQFDIPGK
                     SVLDLFAGSGQLGIEALSRGAEKAVFVDKSAEAFSVVKQNLKTCGLSDKATVINSDSF
                     SFLRSTAEKFDIILIDPPYSRQMAETALSLVDGVLKDGGIVMCETDYREDLPEEVGDI
                     KKIKEYKYSKTKLTTYKK"
     gene            complement(1123..1779)
                     /locus_tag="ACZPE2_02505"
     CDS             complement(1123..1779)
                     /locus_tag="ACZPE2_02505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1318751.1"
                     /translation="MINSVIFDLDGTLLNSLDDLADSTNYTLRTAGYPERTRDEVRRF
                     VGNGIYKLIERAVPTGTDKAEIDKCFDIFCRNYKKNMANKTKPYPGITDMLKTLYENG
                     FKLAIVTNKADFAAQELCGEMFGDYVKTVVGSVKERRNKPYPDNVLFAMDKMGAEKSE
                     TVYVGDSEVDVETAKNSGLSVIAVLWGYRDIDVLKKAGAEKTAKNAEELKNILLNLKK
                     "
     gene            complement(1776..>2636)
                     /locus_tag="ACZPE2_02510"
     CDS             complement(1776..>2636)
                     /locus_tag="ACZPE2_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1318752.1"
                     /translation="LEQWLSEKKGKKVTVFVPARGEQLEIVNMCRKNAEEKLAIKKGR
                     TGREIAVLDELKDLLGLKKTPEYIESYDISHTAGQDSVAGMIVFKGGKPYRKAYKRFS
                     IKSFDGNDDYRAMNEVLTRRFSEYEKSKDSTEGFGKLPDLILLDGGVGQVHAVEPVLR
                     EFGLKIPLFGMVKDNRHRTRAISGDGGEIAINSKRQVFTLVSEIQNEVHRFSVAYHHQ
                     KHAKRGLSLSLTEIEGVGEKRASALLKYFKTMTAIKNAEVDELSKAPGITSAVAQNIY
                     DYYRTKDCLK"
BASE COUNT          658 a          649 c          506 g          823 t
ORIGIN      
        1 tatttgctgt ctctcagttt atttttttat ttttttgatg atgtcctcac gtatttcagc
       61 aggaacgaat gtgctaatgt caccgccgag ctcacaaacc tgctttacca cgctggagct
      121 taagtacata ttctcggctc gcgttgtaat aaataccgtt tcaagctcgt tattgagttt
      181 tttatttgtg agcgcctgtt gaaattcgta ctcaaaatcc gaaaccgctc gaaggccctt
      241 tactatggca accgcctctt tgcggcgcgc ataatcggca agcaaaccgt cgtatcggtc
      301 cacatcgacg tttggcatat ctgcggtaca gcgcttcaaa agctcaactc tctcctccga
      361 cgtgaaggca taattatttt ttttatgata atttgtcatt acgacaacga tgaccttatc
      421 aaacattttc gcacttctgc ggataatgtc aaggtgcccc attgtaacag ggtcaaaact
      481 gccgggacat ataaccgttt tcaagaaaac ccctccgaat tactttttat aggtagtgag
      541 tttagtcttg gaatatttat attccttaat ttttttaata tcaccgactt cttccggcaa
      601 gtcctcgcgg taatcggttt cacacattac aatgccgccg tcttttaaaa caccgtcgac
      661 aagagacaga gctgtttctg ccatttgcct gctatacggc ggatcaatga gaattatatc
      721 aaacttttct gcggtgctgc gaaggaaact aaagctgtcg gaatttataa ccgttgcttt
      781 atccgataaa ccgcaggttt taaggttttg cttaacaacg gaaaaagcct ctgcgctttt
      841 atcaacaaac acagcctttt cggcaccgcg tgaaagagcc tcaattccga gctgacccga
      901 ccccgcaaaa aggtcaagca cagatttccc cggaatatca aactgtatta tactgaacat
      961 agcctctttt accctgtcgg tagtgggacg caccacttct tcgccctcaa gagtcacaag
     1021 cttacgtcct ctggctatac cggtaataac tctcataaga tctctccata aaatgcttct
     1081 aatattattt cattatttta tcacaattgt caacagtttt cactattttt ttaaatttaa
     1141 gagtatattc tttaattctt cggcattttt cgcggttttt tccgctccgg cttttttgag
     1201 aacgtctata tcgcgatagc cccaaagcac ggcaataaca ctcagccccg aattttttgc
     1261 cgtttcgaca tcaacctcgc tgtcgcctac atatacggtc tcgctctttt cggcacccat
     1321 cttatccata gcgaacaaaa cattgtcggg atagggcttg tttctgcgct cttttacgct
     1381 tccgacaacg gttttaacgt aatcgccgaa catttcgccg caaagctcct gtgcggcaaa
     1441 atcagcctta tttgtaacta tggcaagctt aaagccgttt tcataaaggg tctttagcat
     1501 atccgtaatt ccgggatacg gttttgtttt atttgccata ttttttttgt aattgcggca
     1561 aaatatatca aaacacttat ctatttctgc cttatcggtg cctgtgggaa cggctctctc
     1621 gataagctta tatataccgt ttccgacaaa cctgcgcacc tcgtcgcgcg tcctttccgg
     1681 gtagcccgcg gttctgagcg tgtaattagt gctgtctgca aggtcgtcga gagagttaag
     1741 aagagttccg tcgagatcga atataaccga gttaatcatt ttaaacaatc ctttgttctg
     1801 taataatcat atatattttg tgcaacggca gaggttatcc ccggagcctt tgaaagctca
     1861 tcgacctcag cgtttttaat tgcagtcatg gttttaaaat attttaaaag cgcggatgct
     1921 cttttttctc cgacgccctc tatctcggta agagatagcg aaagcccgcg ttttgcatgc
     1981 ttttggtgat gatatgccac agaaaatcta tgcacctcat tctgtatttc ggaaacgagc
     2041 gtaaacacct gtcgttttga atttatggct atttcaccgc cgtcacccga aatggcacgc
     2101 gtacgatgtc gattatcctt taccataccg aaaagcggaa ttttaagtcc gaattcacga
     2161 agcacgggtt ctacggcatg gacctgccca acgccgccgt caagaagtat aaggtccggc
     2221 aatttaccga agccctcggt tgaatccttg cttttttcgt attcggaaaa gcgtcttgta
     2281 agaacctcat tcatagcgcg gtaatcgtcg ttaccgtcaa agctctttat ggaaaaacgt
     2341 ttatacgcct tacgataagg cttaccgccc ttaaaaacta tcatacccgc aacagaatcc
     2401 tgccctgcgg tatgcgatat atcgtaggac tcaatgtact ccggcgtctt ttttaggccg
     2461 agtaaatcct taagttcgtc gagaacggct atctctcttc cggttctgcc ctttttaatc
     2521 gccagctttt cctcggcgtt tttacggcac atattaacta tttcgagctg ttcgcccctt
     2581 gccggaacaa agaccgtaac ttttttgccc tttttctcgg aaagccattg ctccaa
//
LOCUS       DBMDVO010000117         2618 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18459_length_2618_cov_1.432658, whole genome shotgun sequence.
ACCESSION   DBMDVO010000117 DBMDVO010000000
VERSION     DBMDVO010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_2618_cov_1.432658"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="dxr"
                     /locus_tag="ACZPE2_02515"
     CDS             complement(<1..939)
                     /gene="dxr"
                     /locus_tag="ACZPE2_02515"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1318753.1"
                     /translation="MTKNLSLLGSTGSIGTQSLDVARKRGYRVRALSAFSNVKKIEEQ
                     IREFKPDVAALVSEDAAKDLKVRVADTGTKILSGSEGVCECAAYDKSDTVLNSVVGMA
                     GLEPTLTAIKAHKKLALANKETLVAGGRLVMDTAERENVDILPVDSEHSAIFQALQGK
                     PSNRALKKIILTASGGPFFGKTAQELEKVTVNDALKHPNWSMGAKITIDSATMMNKGL
                     ELIEAVWLFGVSPHDVKIVVHRESIVHSAVEYDDNSVIAQLGLPDMRIPIQYALTYPE
                     RYESPVGELDLASLGKLTFFEPDYDTFRCINICREAI"
     gene            complement(936..1805)
                     /locus_tag="ACZPE2_02520"
     CDS             complement(936..1805)
                     /locus_tag="ACZPE2_02520"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1318754.1"
                     /translation="MLTRIIVGVVAALFAVGMLAIRFTPVFPIVVAFFAVMAAFELER
                     VIGVKNKAIHIISLIFAGLIPLYYGFNHYLTERGIFIPALAVVILYILLLFILMITDY
                     EHTKFEDVAAVVVASYFVPWGFSTLIMMRDVGVTYPELYDKHYGLFFILLGLFSAWLT
                     DSLAYFAGRFFGKHKMAPNISPKKTIEGAVGGAFAGVLSSVILFLVFDSKYFTVHLFT
                     WWEIAIISLILCVIGMCGDLSASVLKRNFGVKDYGKLFPGHGGVMDRFDSALFVLGAL
                     YAIIIIYSRIKLA"
     gene            complement(1877..2611)
                     /locus_tag="ACZPE2_02525"
     CDS             complement(1877..2611)
                     /locus_tag="ACZPE2_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR1318755.1"
                     /translation="MDYDNLPTFEVLPKHVGVIMDGNGRWAKKRDLPRYKGHIEGAKT
                     FRKIGEFAADLGIKYITFYAFSTENWKRPKEEVSAIMNLFREYLKEALGRVEEDRKNG
                     IRISFIGSREGIPADIRLLMKQVERIGRKEDRIMLNIAVNYGGRQEIAESVREIAEDV
                     KKGKIKPSEIDMDTISSHLYTAGMPDPDLIIRPSGEQRLSNFLLWQSAYAEFWYSDVL
                     WPDFTEEDFVEALRAYEHRSRRFGGV"
BASE COUNT          702 a          678 c          584 g          654 t
ORIGIN      
        1 tatcgcctct ctgcaaatat ttatgcagcg aaatgtgtcg taatccggct caaagaacgt
       61 gagtttaccc aaagaagcca aatcaagctc gcctacggga gactcatatc tttcgggata
      121 ggtcaaagca tactgtatcg ggattcgcat atcgggaagc cctaactgcg ctataacgga
      181 attgtcgtca tattcaacgg cagaatgaac aatgctctca cgatgaacga cgattttaac
      241 gtcatgcgga cttacgccga aaagccagac ggcctcgata agctccaatc ccttattcat
      301 cattgtagcg gaatctatgg ttatttttgc gcccatagac cagttcgggt gctttaaagc
      361 atcgttcacg gtaacttttt cgagctcctg cgcggtcttg ccgaaaaacg ggccgccgga
      421 ggccgtgaga attatctttt ttaacgccct gttagaaggt ttcccctgaa gagcctgaaa
      481 tatagccgag tgttcggaat ccacgggaag aatatcgaca ttttcccttt cggcagtgtc
      541 cataacaagt ctgccgccgg caacaagagt ctctttattg gcaagcgcca attttttgtg
      601 agccttgatt gccgtcagag tcggctcaag acccgccata ccgacaacgg aattgagtac
      661 cgtatcggat ttatcataag cggcacattc acagactccc tctgagcccg aaagaatttt
      721 tgtgcccgta tcggctacgc gcacctttaa atcctttgcc gcatcctcgg ataccagcgc
      781 cgcgacatcc ggtttgaatt cgcgaatctg ttcctcgatt tttttgacgt ttgaaaaagc
      841 ggacaaagcc ctgacgcggt acccgcgctt gcgggcaacg tcaagacttt gagttcctat
      901 tgagcctgtc gagcctaaaa gtgataaatt ctttgtcatg ccaatttaat ccttgaataa
      961 ataatgatta tcgcatagag cgcaccgaga acaaagagcg cgctgtcaaa tctgtccata
     1021 actccgccgt gacccgggaa gagcttgccg taatccttga cgccgaaatt tctcttgagt
     1081 acagacgccg aaaggtcacc gcacataccg ataacacaga ggataagcga aataatcgct
     1141 atctcccacc aagtaaagag atgaacggta aaatatttgc tgtcgaaaac aaggaagagt
     1201 attactgagc tgaggactcc cgcaaaggct ccgccgacgg caccctctat ggtctttttg
     1261 gggctgatat tcggagccat tttgtgcttg ccgaaaaatc tgcccgcaaa atacgcaagc
     1321 gaatccgtaa gccacgccga gaaaagcccg agcagtatga aaaagaggcc gtagtgctta
     1381 tcgtaaagct ccgggtatgt aacgccgacg tcacgcatca ttatgagcgt tgaaaatccc
     1441 cacggaacaa agtacgaggc caccacaacc gcggcaacgt cctcaaactt tgtatgctcg
     1501 tagtcggtta tcataagaat gaacaaaagc agtatgtaaa gtattactac ggcaagcgcg
     1561 ggaataaaaa tgccccgctc ggtaagataa tgattaaagc cgtagtaaag cggtataaga
     1621 cctgcaaata taagacttat aatatgtatc gctttgtttt tgacaccgat aactctctcg
     1681 agctcgaatg cggccataac ggcaaaaaag gcaaccacta tgggaaaaac cggtgtaaat
     1741 ctgatggcga gcattcccac ggcaaacagc gcggcaacaa cgcctacgat gattcttgta
     1801 agcataaaga gcctcctaaa tattggtttc gacatttagg ttttcggctc tctgccaaaa
     1861 acccgtatcc tttatattat accccaccga atctgcggct gcgatgctca taagcacgca
     1921 atgcctccac aaaatcctct tcggtaaaat ccggccacaa aacatcggaa taccaaaatt
     1981 ccgcataggc cgactgccac aataaaaagt tagaaaggcg ctgctctccg ctcggtcgaa
     2041 taatgagatc ggggtcgggc ataccggcgg tgtagagatg tgacgaaatt gtgtccatat
     2101 caatttccga cggctttatt ttgccttttt tcacgtcctc cgcaatctcg cgcacgctct
     2161 ctgctatctc ctgcctgccg ccgtagttca cggcaatgtt gagcattatg cgatcctctt
     2221 ttctgcctat tcgctcaacc tgcttcatga gaagcctgat gtcggcaggg ataccctcgc
     2281 gagagcctat aaagcttatg cggataccgt tttttctgtc ctcttccact ctgccgagcg
     2341 cttccttgag gtactcgcgg aagagattca ttattgcgga aacctcctcc ttgggacgct
     2401 tccagttctc tgttgaaaaa gcataaaagg ttatgtactt tataccgagg tcggccgcaa
     2461 actcacctat tttgcggaag gtcttggcac cctcaatgtg ccccttataa cgcggcaagt
     2521 cccgtttttt tgcccaacgg ccgtttccat ccattattac gccgacgtgc ttcggcaaga
     2581 cctcaaaggt cggcagatta tcgtaatcca tttacttt
//
LOCUS       DBMDVO010000118         2605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18595_length_2605_cov_5.031550, whole genome shotgun sequence.
ACCESSION   DBMDVO010000118 DBMDVO010000000
VERSION     DBMDVO010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18595_length_2605_cov_5.031550"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            111..584
                     /locus_tag="ACZPE2_02530"
     CDS             111..584
                     /locus_tag="ACZPE2_02530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1318756.1"
                     /translation="MTEKLFELAYTDSMTEVQNRNAYEERLKKLRKPNANISHITVIV
                     VDVNGLKEINDSYGHFSGDDAIKTVAKALRDTIGIKADIYRIGGDEFVCISESNVLPY
                     ISQFKDTIYFINNEKLYNLSVSIGYSRFHEKYSKSIDDIICRADEKMYKDKKSKK"
     gene            603..>2605
                     /locus_tag="ACZPE2_02535"
     CDS             603..>2605
                     /locus_tag="ACZPE2_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318757.1"
                     /translation="MSEKDIKTKPKTTTKAVQNTPKAADRFAKEAVKDIKTVSKDVLI
                     KNIIDKQLDTRQSEQPQRAEVEATEQVENTYYSAVDTVYQKGKHFAENKLKQHRQQAK
                     TRQNQNADTPHTPQNDNVSEVQNTPKQLDNAPKTKESIQAEHKTSAHENAPKVQNNPT
                     AQVKTKEAYMKSQKSDMSEKSADTVKTKANYIKQHKTAEVKDGIKQKPSEIAPKTRVN
                     TSVSSAPTPKTKEQAIKDARKKYVSEKLKTKAEAEKAARQNHTSDMPIHNGNLSKTEI
                     SAPETQRSISDTALPSKSMPKTKESYIRSYGKAPSDLIKTPERAIAMPKQNTHTVTRN
                     VSTGTKKAVKTKDSLLKGTNKAVKTGNSYVGKSAKKKAVKSAKRAAKTQKEITKKAAK
                     QAAKQAKEVAQKSAQAAKATAKAAVKITVKVAQMVAAAAKAIVSALAALGGWAVLLVA
                     LIIVIVVAAIAASPFGIFISDEAADNDSIPISAIVAECNMELSNKLDDIENAAAADRS
                     EIIGEQANWDLVLAIFATKVAGVEDDTVEDVVVITEDKKQKLKNVFWDMHEITSRIET
                     AASGDNSEKVVYITITAKTKEEMIEKYHFTRKQQEALNTLLEQDEVLISAAQSLAVSD
                     ATAQDILKNLPDSLSAERKKVIKAACSLVGKVNYFWGGKSSAIG"
BASE COUNT          965 a          545 c          570 g          525 t
ORIGIN      
        1 ccaagccgca atcaatcgcg gaataaaagg ttgatagaaa ggagaaaggt ataatgtaac
       61 gaccgttaga tgagtcaatt acattatacc aggacgctat gaaaaataaa atgacagaaa
      121 aattatttga gcttgcctat accgacagta tgaccgaagt gcaaaatcgc aacgcttatg
      181 aggaacgctt gaaaaagcta cgcaagccta acgcaaatat ttcacacatc acggttattg
      241 ttgtcgatgt aaacggcttg aaagaaataa acgacagtta cggacatttc agcggtgatg
      301 atgcaataaa gaccgttgca aaggctctcc gagatactat cggaataaag gctgatattt
      361 accgtattgg cggtgatgaa tttgtctgca tatccgaaag caatgtgctg ccgtatatat
      421 cgcaatttaa ggacacgata tattttatca acaatgaaaa gctgtataac ttgtctgttt
      481 cgataggcta ttcaagattc cacgaaaaat acagtaaaag cattgacgat ataatctgcc
      541 gtgccgatga gaagatgtat aaggataaaa agagtaaaaa ataacgccgg aggtgatata
      601 ctttgagcga aaaagatata aagacaaagc cgaaaacaac caccaaggct gttcagaata
      661 ctccgaaagc cgcagaccgc tttgcaaaag aagcagtcaa ggatataaaa accgtgtcaa
      721 aagatgtact tatcaaaaat atcatagata agcagcttga cacacggcag tcggagcagc
      781 cgcagcgagc cgaagttgaa gcaaccgaac aggttgaaaa cacctactat tccgctgtcg
      841 atactgttta ccaaaaaggt aaacattttg cggaaaacaa gttgaagcag caccgtcagc
      901 aagcgaaaac acggcaaaat caaaacgcag atacaccgca tacacctcaa aatgacaatg
      961 tttcagaggt gcagaacaca cccaagcagc ttgacaatgc tccgaaaaca aaagaaagca
     1021 tacaagccga gcataaaaca tctgcacacg aaaatgctcc aaaggtgcaa aacaatccta
     1081 ccgcacaggt taagaccaaa gaagcatata tgaagtcgca gaaatcggat atgtcggaaa
     1141 aatcagctga cacggttaaa acaaaggcta attacataaa acagcacaaa accgccgaag
     1201 ttaaggacgg tatcaagcaa aaaccgagcg aaattgctcc gaaaacaaga gtgaatacct
     1261 ctgtttcctc tgctcccact cccaaaacca aagagcaagc cataaaggac gcacgaaaaa
     1321 aatatgtttc ggagaaactg aaaacaaaag ctgaagctga aaaagcagca cggcaaaatc
     1381 atacttcgga tatgccgata cataacggga atttgtcgaa aacagaaatt tcagcacccg
     1441 aaacacaacg aagtatatct gataccgctt taccctcaaa atctatgccg aaaacaaagg
     1501 aaagctacat acgctcatac ggcaaagctc cgtccgacct cataaaaaca ccggagagag
     1561 caatagcaat gccaaagcaa aatactcata ccgttacccg aaatgtatcg accgggacaa
     1621 aaaaggcggt taagacaaaa gacagccttt tgaaaggcac aaataaagcc gtgaaaacgg
     1681 gcaattccta tgtaggcaaa tctgcaaaga aaaaagctgt taaatcagca aaacgggcgg
     1741 caaagacgca gaaggaaatc acgaaaaaag cggcaaagca agctgccaag caagcgaaag
     1801 aggttgctca gaaatctgca caagccgcaa aggcaaccgc aaaagcggct gttaaaatta
     1861 cagttaaagt cgctcaaatg gtagcggcag cagcaaaagc cattgtttcc gcacttgccg
     1921 cactcggtgg atgggcggtg cttttggtcg ctcttattat cgtaatcgtt gttgcggcaa
     1981 tagcggcatc gccattcggt atatttattt cagatgaagc ggcggataat gacagtatcc
     2041 ccatttccgc tatcgttgca gaatgtaaca tggaactttc aaacaaactc gatgacatag
     2101 aaaatgccgc agcagccgac cgcagcgaaa ttatcggcga acaggcaaat tgggacttgg
     2161 tactcgctat atttgcgaca aaggttgcgg gtgtggaaga tgatacggtc gaagatgttg
     2221 tcgttatcac ggaggataaa aaacaaaagc tcaaaaatgt gttttgggat atgcacgaaa
     2281 taacctcacg catagaaacg gctgcaagcg gtgataactc tgaaaaagtt gtgtatatca
     2341 caattacggc gaaaacaaaa gaggaaatga tagaaaaata tcatttcaca cgcaagcaac
     2401 aagaagcgtt aaatactctt ttggaacaag atgaagtgct gatttccgca gcgcagtcgc
     2461 tcgccgtatc ggacgctacc gcacaggata ttttgaaaaa cttacccgat agtttatcag
     2521 cggagcgaaa gaaagttata aaagccgcct gctcgcttgt aggcaaggtg aattactttt
     2581 ggggcggtaa atcgtctgct atcgg
//
LOCUS       DBMDVO010000119         2604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18620_length_2604_cov_1.210947, whole genome shotgun sequence.
ACCESSION   DBMDVO010000119 DBMDVO010000000
VERSION     DBMDVO010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18620_length_2604_cov_1.210947"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(166..798)
                     /locus_tag="ACZPE2_02540"
     CDS             complement(166..798)
                     /locus_tag="ACZPE2_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR1318758.1"
                     /translation="MKYIGIPLGMWVLFEKSFRKNLVSEFGYNKITAKQISKNAKSRY
                     KSIIRNLPEFEKGDQFKTNIVGCAMLGAFVLSMPKRPSVDELTEYYSKAMMTRFMRWL
                     CRQSGKKKFTAKDIAEMKKTAELKAADRNPYSWNMDFYEYSDGSGYEGRFTKCGICVL
                     MKELGLFDLTPAMCRLDYTMSEAGGATNFVRRYTIASGGSYCDCGYKKKI"
     gene            complement(795..1637)
                     /locus_tag="ACZPE2_02545"
     CDS             complement(795..1637)
                     /locus_tag="ACZPE2_02545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1318759.1"
                     /translation="MKYKIEKNTVQETLIIPLYARKMCSELYPTLYYDETASELIDKI
                     DYDFSDVAEKSKSLMQRFGFLEVAMRQNDLAWEVRDYLSTHPNAAVVNLGCGLDNTGR
                     FCDNGKCKIYNLDFPDVIAVRNQLLPAGEREINIATDLNDLSWLEKIDASDGIVLFAA
                     GVFYYFRTEQVKKLVQAMSNVFGGGRMVFDAAGKTAVKMIAKTWLKESKIKDVSAYFS
                     VSDAKKELSLWSRDMSISSRGYMLGYGDLKNAPVGRFFKFLASVGDNIMKMQIVRLDF
                     GGAK"
     gene            complement(1634..2248)
                     /locus_tag="ACZPE2_02550"
     CDS             complement(1634..2248)
                     /locus_tag="ACZPE2_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1318760.1"
                     /translation="MFWDNVACVYDVFANVINRKTNKELCSVVKNLILPEYEVLECAC
                     GTGLLTKKTAQRCKSLVATDFSEKMLERAKKKCRKYANVHFEKADIMHLDYPDHSFDA
                     VVAANVIHLLDEPYKALHELERVCRPGGKIIIPTYMNRTENGKINGASKAIEKAGANF
                     KRKFTPETYRDFFAAAGYAEASYTLCEGRIPCAVAVLIKKGALL"
     gene            complement(2275..>2604)
                     /locus_tag="ACZPE2_02555"
     CDS             complement(2275..>2604)
                     /locus_tag="ACZPE2_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1318761.1"
                     /translation="KYLFTGDTIWFGADGGYSFISALAESNKLAVRSLGILEQKLKKM
                     CVKPLFITGHTGFTDNFEFAFAHKEKLCSPFKKKVHDPSAPYDAYDESDDTEENAKNG
                     FLKAAGK"
BASE COUNT          694 a          637 c          472 g          801 t
ORIGIN      
        1 tcaagccttg taattgccgt ttggaacggt cagccaagtg ggacaaagaa cacaatagat
       61 tatgccgatc gcaaggatgt taaagttgtt aatgttttag atacaacaat gcaataaaaa
      121 cggcattatt tacagttcct tttaaattcg atttacgaac aaccgttaaa tcttcttttt
      181 atagccgcaa tcgcaatacg agccgccgga cgcaatggta tatcggcgca caaaattcgt
      241 ggctccgcct gcctcgctca tcgtataatc caagcggcac attgcaggcg tcaaatcgaa
      301 cagtccaagc tctttcatca gcacgcaaat tccgcacttt gtaaatcgcc cctcatagcc
      361 gctgccgtcc gaatattcat aaaaatccat attccacgag tacgggttgc ggtcggcagc
      421 ttttaattcg gcagtttttt tcatttctgc aatgtctttg gcggtgaatt ttttctttcc
      481 gctttggcgg cagagccacc tcataaacct tgtcatcatc gcttttgaat agtattcggt
      541 aagttcgtca acgctcggtc ttttgggcat agagagtaca aaagcaccca gcattgcaca
      601 gccgacaatg tttgttttaa actgatctcc cttttcaaat tcgggcagat tacggataat
      661 gcttttgtat ctgctttttg cgtttttcga tatttgtttg gctgtgattt tattatatcc
      721 gaattcggaa acaagatttt ttctgaacga tttttcaaac agcacccaca ttccaagcgg
      781 tattcctata tatttcattt tgcacctccg aaatcaagac gcacaatctg cattttcata
      841 atattatcgc ctacgcttgc taaaaactta aaaaatctgc cgacaggcgc atttttaagg
      901 tcgccgtaac cgagcatata ccctctgctt gaaatgctca tatctctgct ccataaggaa
      961 agctctttct ttgcgtccga aacggaaaaa taagcgctga catcttttat ctttgattcc
     1021 ttaagccagg tctttgcaat catttttacg gccgttttcc cggcggcgtc aaacaccatt
     1081 ctgccgccgc caaacacatt cgacatagcc tgaacaagct ttttaacctg ttcggtgcga
     1141 aagtagtaaa acacacctgc cgcaaaaagc acaataccgt ccgatgcgtc gattttttca
     1201 agccaactta aatcattcaa gtcggtagca atgtttattt cacgctcgcc ggcaggaagt
     1261 agttggtttc gcacagctat aacatcggga aaatcaaggt tgtagatttt gcattttccg
     1321 ttatcgcaaa agcgtcctgt gttatcaagt ccacagccta agtttacaac cgccgcgttc
     1381 gggtgcgtac tcaaataatc ccgaacctcc cacgcaaggt cgttttgacg catcgcaacc
     1441 tccaaaaatc caaatctttg cataaggctc tttgattttt ccgcaacatc cgaaaagtca
     1501 taatcaattt tatcaatcag ttcggacgct gtttcatcat aatacaatgt gggatacagt
     1561 tcggaacaca ttttccgtgc ataaagcgga ataataagcg tttcctgtac agtgttcttt
     1621 tcaattttat atttcatagt aacgctcctt tttttatcaa caccgccacc gcacaaggaa
     1681 tccgtccttc acataaagta tagcttgctt ctgcatatcc cgcagcagca aaaaaatctc
     1741 tgtatgtctc cggagtgaat tttctcttaa agttagctcc ggctttttcg attgcctttg
     1801 aagcaccatt gatcttgccg ttttcagtac ggttcatata tgtcggaata atgatttttc
     1861 cgcccgggcg gcatacccgt tcaagctcgt gcagagcctt gtacggttcg tccagcaagt
     1921 gaatcacatt tgcagcaact actgcatcaa aactatgatc gggataatca aggtgcatga
     1981 tgtccgcttt ttcaaaatgt acattggcat atttacggca ctttttcttt gctcgctcca
     2041 acattttttc ggaaaaatcc gttgcaacaa ggcttttgca ccgctgtgct gttttttttg
     2101 tgagtaatcc agtgccgcag gcacattcca aaacctcata ttcgggcaga atcagatttt
     2161 tcaccacaga gcagagttcc ttatttgttt ttcggttaat aacatttgca aaaacgtcat
     2221 atacacacgc aacattatcc caaaacatag cttgcctccg ttatttcagt cctcttattt
     2281 ccctgccgcc tttaaaaatc cgttttttgc attttcctcc gtatcatcag actcatcata
     2341 ggcatcgtac ggtgccgacg gatcatgaac tttcttttta aacggtgaac acagcttttc
     2401 cttatgtgca aatgcaaatt caaagttgtc ggtaaaacct gtatgtcccg taataaacag
     2461 cggcttaaca cacattttct tcagtttttg ctcaagaatc ccgagcgatc tcaccgccag
     2521 cttattgctt tccgcaagtg ctgaaataaa gctgtaaccg ccgtctgcac caaaccaaat
     2581 cgtatctccg gtaaacagat attt
//
LOCUS       DBMDVO010000120         2582 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18853_length_2582_cov_1.343813, whole genome shotgun sequence.
ACCESSION   DBMDVO010000120 DBMDVO010000000
VERSION     DBMDVO010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18853_length_2582_cov_1.343813"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(45..524)
                     /locus_tag="ACZPE2_02560"
     CDS             complement(45..524)
                     /locus_tag="ACZPE2_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318762.1"
                     /translation="MKKIVSILLAVMMIAAIAVPSFAATITKESDPKTADVQVKTKTT
                     DKENNDPENYTVTIPAEITVAWNDTAAQDASYTVDSQLKLGAKLKVSAAANDAGKMTN
                     AATDKFLTFTVAGGDVAEYPELNNAAKSATTVTIADFNAPIAEYVGHMTYTVEYVAA"
     gene            complement(568..1053)
                     /locus_tag="ACZPE2_02565"
     CDS             complement(568..1053)
                     /locus_tag="ACZPE2_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318763.1"
                     /translation="MKKLLSIVLAAAMLAAVSVPSFAATAEINQDTADGKGVATVKTD
                     TTNVPADGYFTVTYPAEMSVKWGAETTAFTYNVKSTLKTGKCLSVSVASEAGKMKPAT
                     DNGYTIPYAFSGDTSVKTAAEVVNNTYNINVDVAAAAWNATITDYSDATVTFTAAVVD
                     Q"
     gene            complement(1081..1560)
                     /locus_tag="ACZPE2_02570"
     CDS             complement(1081..1560)
                     /locus_tag="ACZPE2_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318764.1"
                     /translation="MKRILSIVIAAVMLLAISVPAFAATATIDQSTSGGVGNSSVVTD
                     RSGISGTFTVSYPAETTIYWGTESTEIGYTVSSTLVSGNNLRVTVAQDSANLHTGGGR
                     LLPYTIAGDTNITTGHETVTDSAYSVNVNITKENWGSVSIDEYEDTLTFTASVEAIA"
     gene            complement(1599..2105)
                     /locus_tag="ACZPE2_02575"
     CDS             complement(1599..2105)
                     /locus_tag="ACZPE2_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318765.1"
                     /translation="MKKLLSIVLAAAMLAAVSVPSFAADKTLTSDHTSDTAKVITSTK
                     TGPDGPEATSYTVTFPAETTIYWGVESTDFTYNVKCQLKSNDRLNVTVAKEDGTTDLK
                     AIASGNTATLPFSVAGTTAYTSASAVVADEAVNAAVNIASADWAKVPVDEYEGNLTLT
                     VSIAPATI"
     gene            complement(2127..>2582)
                     /locus_tag="ACZPE2_02580"
     CDS             complement(2127..>2582)
                     /locus_tag="ACZPE2_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318766.1"
                     /translation="AAASVPAFAANPITKETDNTGTAIVKTSTKTGPDAPDDNAVKYT
                     VTIPADTTIYWGAEETALDGFSVESHLKYGQKLKIGVEGSRLMKLSEDANETLAYDFS
                     ADSNLEFIADGPVINPAKALTLKVKVAADDWNNAIVGEYSDTLTFTVSL"
BASE COUNT          658 a          551 c          641 g          732 t
ORIGIN      
        1 aaaccacctc cttaaaaatt ttaaaaatca aaatccggca ataattatgc agcaacgtac
       61 tctactgtat aagtcatgtg gccgacatac tcagctatgg gagcgttgaa gtcagcgatt
      121 gtaacggttg ttgcagactt agcagcgttg ttaagctcgg gatattcagc aacgtctccg
      181 cctgctacgg tgaaggtgag gaatttatcg gtagcagcat ttgtcatttt tcctgcatcg
      241 ttagcagcag ccgaaacctt gagcttagcg ccgagcttaa gctgagaatc aacggtgtaa
      301 gaagcgtcct gagctgcggt gtcattccaa gcaactgtga tctcagcagg aattgtaacg
      361 gtgtagtttt cgggatcgtt gttctcctta tcggttgtct ttgtcttaac ctgaacatcg
      421 gcggtcttgg ggtctgattc cttggttatt gtagctgcga atgagggaac tgctatagct
      481 gctatcatca taacagcaag aagaattgat actatctttt tcataattgt aactccttaa
      541 tgaaataatg tttaactgtt aaacaaatta ttggtcaaca actgctgctg tgaaggtaac
      601 ggtagcatcg ctgtaatcgg tgatggtagc gttccaagct gctgctgcaa cgtcaacgtt
      661 aatgttgtaa gtattgttaa ctacctcggc agcagtctta acggaagtgt cgccgctgaa
      721 ggcgtaagga atggtatagc cgttatcggt agcgggcttc attttgccag cctccgaagc
      781 aacagaaacg gaaagacatt tgccggtctt gagagttgac ttaacattgt aggtaaaggc
      841 tgtggtttca gcgccccatt taacgctcat ttcagcagga tatgttactg tgaaataacc
      901 gtctgcggga acgtttgttg tatcggtctt aacggttgca acacccttgc cgtccgctgt
      961 atcctgattg atttcagctg tagctgcaaa ggacggaact gaaactgccg caagcatagc
     1021 cgccgcaaga actattgaaa gtaacttttt cataatataa ctccttttcg gattaaaaaa
     1081 ttaagctatc gcttcaacag aagctgtaaa tgtaagagta tcttcatact catcaatgga
     1141 aacggatccc cagttttcct tagtaatatt gacatttacc gaatatgcgc tgtcggtaac
     1201 tgtctcatga ccggttgtta tgttggtatc accggctata gtgtaaggaa gaagtctgcc
     1261 gccgcctgta tggagatttg cactgtcctg agctacagta acacgaaggt tatttccgct
     1321 cacaagcgta ctcgatacag tgtaaccgat ttcggtactt tcggtgcccc aataaatggt
     1381 ggtttcggcg ggataagaaa cggtaaaggt accgcttatt ccgcttcggt cggtaactac
     1441 cgaggagtta ccgacgccac cggaagtaga ctgatctatt gtcgcagttg cagcaaaagc
     1501 gggaaccgat atagccaaga gcatcacagc cgcaatgact attgaaagaa ttcttttcat
     1561 aattggttct ccttgtattt tgaaagggtt tccgactctt agattgtagc gggagctatc
     1621 gaaaccgtaa gtgtaagatt gccctcatac tcatctaccg gaacctttgc ccaatcagct
     1681 gaggcaatgt ttactgcagc gttaacagct tcatctgcaa caactgcaga ggctgaagta
     1741 taagctgtgg tgcctgctac gctgaaagga agagtagcgg tattgcctga tgcgatagcc
     1801 ttgaggtcgg ttgtgccgtc ttcctttgct accgtaacat taagacggtc gtttgatttg
     1861 agctggcact ttacgttgta tgtaaagtct gtgctttcta cgccccagta aatagtggtt
     1921 tctgccggga aggttaccgt gtaagaagta gcctcgggac cgtcgggacc ggtttttgta
     1981 gaagtaataa ccttagccgt atcggaggta tggtcgcttg taagcgtctt gtcagctgca
     2041 aatgacggaa cggaaaccgc tgcgagcatt gccgccgcaa gaactattga aagtaatttt
     2101 ttcattgtgg ttatctcctt tcaaaatcaa aggcttacgg tgaaggtaag agtatcgctg
     2161 tactcgccga cgatagcgtt gttccaatcg tctgcggcaa ccttaacctt gagggtaagt
     2221 gcctttgcgg gattgataac ggggccgtca gctataaatt cgaggttaga gtcagccgag
     2281 aaatcgtaag ccaatgtctc gtttgcatcc tctgaaagct tcataagtct tgagccctca
     2341 acgccgattt taagcttctg gccgtattta aggtgagact caacagaaaa tccgtcaaga
     2401 gctgtttcct ctgcgcccca gtaaattgtg gtatcggccg gaattgtaac ggtgtattta
     2461 acagcgttgt catcgggagc atcggggcct gtttttgtgg aggtcttaac aatagctgta
     2521 ccggtattgt cagtctcctt ggttatgggg ttagctgcga aagcgggaac cgaagctgct
     2581 gc
//
LOCUS       DBMDVO010000121         2576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18915_length_2576_cov_1.245657, whole genome shotgun sequence.
ACCESSION   DBMDVO010000121 DBMDVO010000000
VERSION     DBMDVO010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18915_length_2576_cov_1.245657"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="ACZPE2_02585"
     CDS             <1..1935
                     /locus_tag="ACZPE2_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1318767.1"
                     /translation="ENVLEKGSYYLTSREKEMLDLVTAYFDDVILVLNIGSLIDFEEI
                     DAYKDKIGSILIAWQGGMESGNALSDILSGEVTPSGKLSDTIAKRYEDYPSSGSFGAK
                     EYNNYVEDIFVGYRYFETFAPEKVLYEFGFGLSYTNFKIETEKANYSEGKTEFNVKVT
                     NIGSVPGRETVQFYVSAPNGKLGKAKKSLVAFHKTELLSPGDCETFVTVISNDSFASY
                     DDSGKAGHKSCYILESGIYDFYVGNSVRNCEKVYSFTLESTVVTAELSEVMAVSENRA
                     FERFAAVCDGDKIALSKEKVPVRTVSLKNRILSSLPDGKEISDDKGYKLSDVKAGKVK
                     LRDFTAQLSLDELEAISRGEGPMNSSLGAKGNAGAFGGVLKSLREKGIPPIITTDGPS
                     GIRLLSACSLMPCGTAIACSWDESLTEELFTEMGKEMIKKGSDVLLAPGMNIHRNVLC
                     GRNFEYYSEDPYLTGKTAAATVRGIQKNGVSACPKHFAGNNQEYNRNHTDDIVSERAL
                     REIYLKGFEICVKESSPHCIMTSYNKINGVWSHYNYELCTTVLRDEWHFGGMVMTDWW
                     MRYAPSPEFPQLRDNAYRVRAQVDVLMPGFKTAVSKSGGNDGSLLETYGQKDGIALGE
                     LQRTANNVLRFILSTDKKLN"
     gene            complement(2036..>2576)
                     /locus_tag="ACZPE2_02590"
     CDS             complement(2036..>2576)
                     /locus_tag="ACZPE2_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1318768.1"
                     /translation="EYTRESITGLYSVVFQDFKIFSVSLKDNICASSDFDCDRFYACL
                     ENANIKDRAERLADKENTYLYKDLDETGVEISGGEAQKLALARALYKDAPVVILDEPT
                     AALDPIAENEIYSRFNSFVQNKTAIYISHRLSSCAFCDKIAVFDNARLVEHGTHRELL
                     GAGGKYSELWNAQARYYIS"
BASE COUNT          734 a          602 c          623 g          617 t
ORIGIN      
        1 gaaaacgttc tcgaaaaggg cagctattac ctcacgagca gagaaaagga aatgcttgac
       61 cttgtcacgg cttattttga cgacgtcatt ttggttctca atataggcag tcttattgat
      121 tttgaagaga tagatgcata taaggataaa ataggctcga tacttatagc ttggcaaggc
      181 ggtatggaga gcggaaacgc gctttcggat atactctccg gtgaagtcac tccgagcgga
      241 aagctcagcg atacaatcgc aaaacgatat gaggactacc cgagcagcgg cagcttcggc
      301 gcaaaggaat acaacaacta tgttgaggac atctttgtgg gttaccgcta ttttgaaacc
      361 ttcgcccccg aaaaggtgct gtatgaattc ggattcggtc tgtcttatac aaattttaaa
      421 atcgaaacgg aaaaagcgaa ctattccgaa ggcaaaacag aattcaatgt taaggtgacg
      481 aatatcggct cggtgcccgg cagagagacg gttcaatttt atgtttccgc tccgaacggc
      541 aagctcggaa aagcaaaaaa atcacttgtt gcttttcaca aaacagagct tttgtccccc
      601 ggcgactgtg aaaccttcgt aacggtgatt tcaaatgaca gctttgcttc ttacgacgac
      661 tccggcaagg ccggacacaa gagctgctat atactcgaaa gcggaattta cgatttttat
      721 gtaggcaaca gcgtgcgaaa ctgtgaaaag gtgtatagct ttacacttga aagcacagtt
      781 gtaaccgccg aactatccga agttatggcg gtcagcgaaa accgcgcgtt tgaacgcttt
      841 gccgctgttt gcgacggcga caaaatcgct ctgtcaaagg aaaaagtgcc tgttcgcacc
      901 gttagtctga aaaatcgaat actctcttct ctgcctgacg gaaaagaaat cagcgatgac
      961 aaaggctata agctctccga tgtaaaggca ggaaaagtaa agctgcgaga ctttacagct
     1021 caactttcac tcgacgagct cgaagcaatc tcacgaggcg agggacctat gaacagctca
     1081 ctcggagcaa agggtaacgc gggtgctttc ggcggcgttt taaagtcgct cagagaaaag
     1141 ggaatccccc cgataatcac caccgacggt ccgtccggaa taagacttct ttccgcctgt
     1201 tcgctgatgc cctgcggtac ggcgatagcg tgttcttggg acgaaagtct caccgaggaa
     1261 ttattcacgg aaatgggcaa agagatgata aaaaagggca gcgacgtgct gttggcaccg
     1321 ggtatgaata tacataggaa cgtgctgtgc ggcagaaact tcgaatacta ttccgaggac
     1381 ccgtacctca cgggtaaaac cgctgcggca acggtcaggg gcatacagaa aaacggcgtt
     1441 tcggcgtgtc cgaagcactt tgccggcaac aatcaggaat ataaccgcaa tcacaccgac
     1501 gacatagtgt ccgaaagggc acttcgcgaa atctatctca agggctttga aatatgcgtt
     1561 aaggagtcgt ctccgcactg cattatgaca agctacaaca agataaacgg cgtttggagc
     1621 cactacaatt atgagctttg taccaccgtt ttgcgtgacg aatggcactt cggcggtatg
     1681 gttatgacag actggtggat gcgctacgct ccgtcacccg aatttccgca gctgagagac
     1741 aatgcatatc gcgtcagagc gcaggttgac gtgcttatgc ccggatttaa aaccgcggtc
     1801 agcaagagcg gcggaaacga cggctctctg cttgaaacat acggtcaaaa ggacggaata
     1861 gccttaggag aactgcagag aaccgcaaac aacgttctcc gctttatcct ctcaaccgac
     1921 aaaaagctaa actgaataaa agccgtataa catataaaaa gactgccttt atgccgtgtg
     1981 acgtacgtaa tcacatttga taaatgcagt cttttttatt tattcgccga gtcctttatg
     2041 agatataata ccttgcctgc gcattccaaa gctcgctgta tttgccgcct gcacccaaaa
     2101 gctcgcgatg cgttccgtgc tcgacaagcc gcgcgttgtc aaatacggcg attttatcgc
     2161 agaaggcgca gcttgaaagc ctgtgagaaa tataaatcgc cgttttattt tgaacgaagg
     2221 aattgaaacg gctgtatatc tcgttttccg caatggggtc gagtgccgcc gtgggctcgt
     2281 ccaaaataac gacgggagcg tccttataca gtgccctcgc aagagcaagc ttttgcgcct
     2341 caccacccga tatttcaacg cctgtttcgt ccaagtcctt ataaagatat gtgttttcct
     2401 tatccgccaa gcgttcggct ctgtccttaa tatttgcatt ttcgaggcaa gcataaaatc
     2461 tgtcgcagtc gaaatcggag cttgcgcaaa tattatcctt aagtgatacg gaaaatattt
     2521 taaaatcctg aaagaccacg gaataaagac ctgtaatgct ctctcttgtg tattcc
//
LOCUS       DBMDVO010000122         2571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18966_length_2571_cov_1.272065, whole genome shotgun sequence.
ACCESSION   DBMDVO010000122 DBMDVO010000000
VERSION     DBMDVO010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18966_length_2571_cov_1.272065"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="ACZPE2_02595"
     CDS             complement(<1..480)
                     /locus_tag="ACZPE2_02595"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1318769.1"
                     /translation="MKRAKRILSFLLMLLVAISASVLYVSAISADTDEVFSGIDVSVY
                     QGDIDFEQVKNSGVEVVYIRAGYGFSVTDPKFEENYTNATKAGLKCGAYYFVTARNTE
                     QAYLQATRFAELISGKTFAARPAMDFEEFGSLGKSGINIVGLAFMQKLRDLTGIVPIL
                     "
     gene            641..955
                     /locus_tag="ACZPE2_02600"
     CDS             641..955
                     /locus_tag="ACZPE2_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318770.1"
                     /translation="MKLAILYQQSEPTAKDGIKELVEIKEDCVAASQALKIKGLVRIN
                     CREDKNGVFKIFDFNAKPNITGGVRPHRKNQDCLTMIAARAAGLTYRDLLLKMLGTAW
                     TV"
     gene            complement(1284..1359)
                     /locus_tag="ACZPE2_02605"
     tRNA            complement(1284..1359)
                     /locus_tag="ACZPE2_02605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Met,seq:cat)
     gene            complement(1525..2499)
                     /gene="bsh"
                     /locus_tag="ACZPE2_02610"
     CDS             complement(1525..2499)
                     /gene="bsh"
                     /locus_tag="ACZPE2_02610"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR1318771.1"
                     /translation="MCTAATYKTNDFYFGRTLDYEFSYGDEITVTPRNYPFKFKFAEP
                     LKSHYAIIGMAYVADNYPLYYDAVNEKGLAAAGLNFVGNAYYGNEKSGKCNVAQCEFI
                     PWLLCRCASVDEAKKLLSNVNITNTPFNESLPAAQLHWIIADKSGCIVVESVKDGLKV
                     YENPVGVLTNNPPFDMQLQNLNNYMSLSAADPKNTFAPDINLSTYSRGMGALGLPGDL
                     SSQSRFVRAAFVKANSLSEDGEEKSVSQFFHILNSVSQPRGCCKLESGKYEITLYTSC
                     CNTDKGIYYYTTYDNHQISAVDMHRENLDSSALIRYSPAKDEQINFQN"
BASE COUNT          693 a          599 c          572 g          707 t
ORIGIN      
        1 aagaatcggc actattcccg taagatctcg gagcttttgc ataaatgcaa gccccacaat
       61 atttatgccg ctttttccga gactgccgaa ttcctcaaaa tccatggcag gtcttgcggc
      121 aaaggttttt ccgcttataa gctcggcaaa gcgcgttgcc tgcaaatatg cctgctcggt
      181 atttcgtgcg gtcacaaaat agtacgcgcc gcatttaaga ccggcctttg tcgcgttggt
      241 gtaattttcc tcgaatttcg ggtcggttac ggaaaaaccg tatcctgctc ttatatatac
      301 aacttcgact ccgctgtttt taacctgttc aaaatctata tctccctgat aaacgctgac
      361 gtctattccc gaaaaaactt cgtcggtgtc ggcggaaatt gccgaaacat acagcacaga
      421 tgccgaaatc gcaacaagga gcattaaaag aaaagatagt attcgttttg cccgcttcat
      481 aaaaaatcac agcctttctg atgtttaaag cctcaatgca ttatatttta aacagcacgc
      541 gagtgtgact ttgcttgcct gccgtaagga cgataccctt gaaaaaccac ggtatcggcg
      601 ataaaataac gctgaaataa aaaattcgga agcgagaaag atgaaacttg cgattttgta
      661 tcaacaaagc gaaccgactg ccaaagacgg cataaaggag cttgttgaaa taaaagagga
      721 ttgtgttgcc gcgtcacagg ctttaaaaat aaaaggactt gtacgcatca actgccgcga
      781 ggataaaaac ggtgtgttta aaatatttga ttttaacgcg aagccgaata tcaccggcgg
      841 tgttcgtccg cacagaaaaa atcaggattg cctcaccatg atagcggcgc gtgccgcagg
      901 acttacgtac cgcgacctgc ttttgaaaat gctcggaacc gcttggaccg tgtaagccga
      961 aacgccaccg ttttacgcac cgccggggtg cgccgaataa aaaaagcact cagctttacg
     1021 ctgaatgctt tatatggttg cggggacagg acttgaacct gcgacatccg ggttacatct
     1081 cacaaggctg cgccttgctt gcccctcaga aatatagtcg catatgctcg cttggagcgc
     1141 atcgcaatgc tccttatttc ttcacctcag cggcacacct taatctgcca caggcagcgg
     1201 cgcaccgctt cgccccaacg agcaccgctc atcgtgctca taacccgaca aaaaaagaac
     1261 tgtcataaaa gacagttcct aaatggttgc ggggacagga cttgaacctg cgacctccgg
     1321 gttatgagcc cgacgagcta ccaactgctc taccccgcga tattaagttg tgccctcttt
     1381 cagagtgcct aactattata ttatcattat atgcgtttgt caagagtttt atttgtattt
     1441 tatatttttc ggtatgtttc ggttttacag cgattaaaaa ggcggcgaca tctcaaaata
     1501 agagccgccg cactttgtaa tttttcagtt ctgaaaattt atctgctcgt cttttgcggg
     1561 cgaatagcgt atcaaagcgg aagaatcgag attttcgcga tgcatatcaa cagccgaaat
     1621 ttgatgattg tcatacgttg tgtaatagta aattccctta tctgtattgc aacaagatgt
     1681 gtaaagcgta atctcgtatt tgccgctttc cagcttgcag caaccccttg gctggcttac
     1741 cgaattgaga atatggaaaa actgacttac gcttttttct tcgccgtcct cggacaaaga
     1801 atttgccttg acgaacgccg ctctgacaaa cctcgactgc gaagagagat ctccgggaag
     1861 tccgagcgcg cccataccgc gagaataagt cgagagattt atatcgggag caaatgtgtt
     1921 tttcgggtca gccgcgctga ggctcatata gttgtttaaa ttttgaagct gcatatcaaa
     1981 cggcggattg tttgtaagaa cgcctacggg attttcgtag actttaagtc cgtcctttac
     2041 ggactcgact acgatacagc cgcttttgtc ggctattatc cagtgaagct gtgcggcggg
     2101 gagcgactcg ttaaacggcg tgtttgtgat atttacattt gagagcaatt ttttcgcttc
     2161 gtcaacgctt gcgcatcgac ataatagcca cggtataaat tcacattgcg caacgttgca
     2221 tttaccgctt ttttcgttac cgtaatatgc gttgccgaca aaattaagtc ctgccgcggc
     2281 aagtcccttt tcgtttactg cgtcgtagta gagcggataa ttatccgcaa catatgccat
     2341 acctattatg gcatagtggc tcttcagagg ctccgcgaat ttgaatttaa acgggtaatt
     2401 acgcggcgtc acggtaattt cgtctccgta tgaaaattca tagtcgagcg tgcggccgaa
     2461 ataaaaatcg tttgttttgt atgttgctgc ggtacacata ttaagtcctc ccgaaatatt
     2521 tgatttttac agagaaaatg ctatacttat gtttgcctga aaaaacggaa c
//
LOCUS       DBMDVO010000123         2568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_18993_length_2568_cov_1.162140, whole genome shotgun sequence.
ACCESSION   DBMDVO010000123 DBMDVO010000000
VERSION     DBMDVO010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_2568_cov_1.162140"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="ACZPE2_02615"
     CDS             <1..186
                     /locus_tag="ACZPE2_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1318772.1"
                     /translation="GAGALLAKCIKKCELVAYEDLQSEALRRLYVEDMPLVVVIDSEG
                     NNLYEEGRKEYLESVKK"
     gene            279..1514
                     /locus_tag="ACZPE2_02620"
     CDS             279..1514
                     /locus_tag="ACZPE2_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase family protein"
                     /protein_id="HIR1318773.1"
                     /translation="MKKIRCILLALIIAALFLVPVSAETGVINALDYGFNNNGTGLND
                     KIMEKYIRDDSATPIYFPSGTYLFSETISFPEQCYVTLAANAEFKLSSTAVRDYFITL
                     RRGFVGSGYTFNSYIKGGYINANNCAKNGIGIYKTRHVEFSDFVLKNVLEKGIVTRTE
                     ERADGQSYIRNVLIENDFGIKGTIGVYDNAHDTRCEMVEVVNFETAFYTISGRFTQCN
                     AWLRDSSLVENSVYAVIDGYHILFDSPQVDTYRYGFKIMNPRYNVLITNMLWITNGGV
                     YTPELRERFPRVIFNADYDTGSYLVSGLKINNEDNLEFSNRELFRSKFLNVRTVGDFD
                     PYETIKYFRNDNDERTGKAEGDLDSLRIGLYECSLFGETGALEVKKTGNITLQRFMGD
                     KTFAYRTNKGEGYTDWIKK"
     gene            1895..>2568
                     /locus_tag="ACZPE2_02625"
     CDS             1895..>2568
                     /locus_tag="ACZPE2_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1318774.1"
                     /translation="MKLSSFLHFAAFGLKTILFRKKSPILGTVIVTDKCNLHCKHCSV
                     NNITAIVHPYEQIRQEMQRLYDMGVRILFFCGGETFLWKDGERTLRDLVIEAKSMGFI
                     IVNVVTNGTFPIDLPEADLILLSLDGDKQRHNEIRGDTYDTIMKNISNAAADNICFYM
                     AINQINKDHVRDVCLTARDTKNVRAVSFNFHTPYPDTKKLALTKEEKAKCCDTITQMM
                     KEGAPV"
BASE COUNT          781 a          534 c          607 g          646 t
ORIGIN      
        1 ggcgcaggcg cacttttggc taagtgcatt aaaaagtgcg agcttgtggc ttatgaggac
       61 cttcagtccg aggcgctcag aagactctat gttgaggata tgccccttgt tgtcgtgatc
      121 gactctgagg gaaataacct ttacgaagag ggcagaaaag aatacttaga gagcgtgaaa
      181 aagtgatttt taagcggcgg aaaattccgc cgcttttttg ttgacttttt ttagttaatg
      241 caatataatt aaatcaacaa aataacggag ggaaaaacat gaaaaagatt agatgtattt
      301 tgcttgcact catcatcgcg gcgctttttt tggtgccggt atcggctgag acgggcgtta
      361 tcaacgcgct tgactacggc tttaacaaca acggcacagg gttaaacgac aaaataatgg
      421 aaaagtatat aagggacgat tccgccacgc cgatctattt cccgagcgga acatatcttt
      481 tttccgaaac gataagcttt ccggagcagt gctatgtcac gcttgccgca aatgctgagt
      541 ttaaactttc aagcactgcg gtgagggact atttcataac gctccgccgc ggctttgtcg
      601 gctcgggata tacgtttaat tcgtacatca agggcggata catcaatgcg aataactgcg
      661 cgaaaaacgg tatcggaata tataagacga ggcatgtcga gttctcggat ttcgttttga
      721 aaaatgtgct cgaaaagggc atcgtcacaa gaacagagga gagggcggac gggcagtcct
      781 atatcagaaa cgtcctgatc gagaatgatt tcggcataaa gggtactata ggcgtttatg
      841 acaatgcaca cgacacacgc tgtgaaatgg tcgaggttgt taattttgaa acggcgttct
      901 atacaatcag cggaagattt acacagtgta acgcatggct tcgtgacagc tccctggttg
      961 aaaactccgt ctacgcggtg atagacggtt atcacatttt gttcgactcg ccccaggtcg
     1021 atacctaccg ctacggattt aagataatga acccccgcta taatgtgctt atcacaaata
     1081 tgctctggat aaccaacggc ggagtttaca cgcccgagct tcgggaaagg ttcccgagag
     1141 tcattttcaa tgcggactac gacacgggaa gctatctcgt ttccgggctc aagatcaaca
     1201 acgaggacaa tctggagttt tcaaacaggg agcttttccg ctcaaaattc ctaaacgtca
     1261 gaaccgtcgg cgactttgat ccgtatgaga caataaaata tttcagaaac gataacgacg
     1321 agagaaccgg gaaagccgag ggcgaccttg attcgttaag aatagggctt tacgagtgct
     1381 cgcttttcgg cgaaacggga gctcttgaag ttaagaaaac aggaaacatc acccttcagc
     1441 ggttcatggg cgataaaacc tttgcctaca ggaccaacaa gggcgaaggg tatacggact
     1501 ggataaaaaa ataaaataaa cgctgcgctt ttgccgtgtg cagataagac agtaatttga
     1561 tagaactaca aaaccggcat ctgtcagaca ggattggaca acaaaacagg cggcattcaa
     1621 cttcggttgg gtgccgcccg tttttataca tctgatgatt gagaatcgct tattttcttc
     1681 tctgcggggc attattcaaa agcgaacaca tctgacttca tctgttcgca cggattaagc
     1741 ctgaagcagg aaaagagacc gaaatggagc ggatgaaccg attacgccga tattctgaaa
     1801 cgcgtttcac tatcttgaaa aaacagaaca gaatgcgtat aatacaatgt gtacatacaa
     1861 ttcaaggtta tctgattaga gctatggagg ggaaatgaaa ctatcatctt ttttgcattt
     1921 tgccgctttt ggcttaaaga caattctgtt tagaaagaag tcgccgatcc tcgggactgt
     1981 aattgtcaca gacaaatgca acttacattg taagcattgt tctgtcaaca acatcacggc
     2041 aatcgtccat ccgtatgaac agatacggca agaaatgcag cggctgtatg acatgggtgt
     2101 ccgaatattg ttcttctgcg gcggtgaaac attcctttgg aaagacggcg aacgcacact
     2161 gagagatttg gtcattgaag cgaaaagcat gggatttatc atcgtcaatg tagttacaaa
     2221 cggtacattt cccattgatc tcccggaagc ggatttgatt cttctgagct tggacggaga
     2281 taagcagcgt cataatgaaa ttcgaggcga tacctacgat accattatga agaacataag
     2341 caacgctgcc gcagacaata tctgtttcta tatggcaatc aatcaaatca acaaagatca
     2401 tgtacgagat gtctgtctaa cggctcgtga cactaaaaat gtccgtgcag tatcattcaa
     2461 tttccataca ccatatcctg acacaaaaaa attagctctg actaaagagg aaaaagccaa
     2521 gtgctgtgat acgataactc aaatgatgaa agaaggcgca cctgtatt
//
LOCUS       DBMDVO010000124         2566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19019_length_2566_cov_1.164706, whole genome shotgun sequence.
ACCESSION   DBMDVO010000124 DBMDVO010000000
VERSION     DBMDVO010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19019_length_2566_cov_1.164706"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(104..1441)
                     /locus_tag="ACZPE2_02630"
     CDS             complement(104..1441)
                     /locus_tag="ACZPE2_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1318775.1"
                     /translation="MTDNINASSGDKAQFYAERLAKMIRCKTVSEKNDFKPEEFLKLR
                     KVIAELFPLVTQKAEFTVFGDDAYLYKLKGKDETRNVMVMSHHDVVEATGDWQEEPFG
                     GIIKDGKLWGRGTVDTKTPLFAEFSAIEELLFEGFEFPVNVYLFSSHNEETGGDGAVL
                     ALDYFNKNHITFDWISDEGGAVIDPPMAGISKKCAMMAVHEKGRCIAELTAKKSKGHV
                     GLAGNHQTPVMRMVSFIAEANEKKPFIRKLHPEVRGMFEAMAPNMKFPMKFVFSNLRL
                     FSGLLIKLMPKLNDAAGEMLGTGCTFRKLYTEEDGSCKGEAFLRCINDEDLAKDLDAL
                     KEIAAKYEVEIVVRDSDNEYYKPTSLESDGYKYVRKIVQSVFDYAAAVPFILPAGTDA
                     RRFTGLSDSVIRFAPIDIDGQQYASVHSENENINVDKIPLAVKFYKELLRNYK"
     assembly_gap    1471..1480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1481..>2566)
                     /locus_tag="ACZPE2_02635"
     CDS             complement(<1481..>2566)
                     /locus_tag="ACZPE2_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1318776.1"
                     /translation="NGRRIPLMQKAAVPFGLCAFLMFFVPLNYVSGFNAVWIAAFMWG
                     YYFFFTLYMIPHNALIPELVQDGPMRVNAYTISSFFFVTGSALGYVSPLIVNGFKSAG
                     MATLGAWRMTFGIFTAIGIVLLIIPAFTIKEKDYVHSVIPNVTLKDSLKHAFSNKHFR
                     IVTLGQLLENTGMAFFQACIMYYVTSLMGLAETYSVLILAISIAGSLALYPLVNKFAK
                     RKGKKLPVILGCIVFSIAEIVICFSDKIPGNKLVLACVFALFVSFPFAVLNVLPGAMM
                     ADIIQYDTVVTGINQEGIFGAAKSFIVKMGNSLAIMIVPSLVVTGAAAGENVGVLGLK
                     LTAVAGSLFCILAIFVLLRYREKEVLAT"
BASE COUNT          683 a          660 c          540 g          673 t
ORIGIN      
        1 ccccgttttg tctgtaaatt caatcccact gtttccggtt aaaatcaaag cgccccggtt
       61 cagtcgagcg tttgccggcc gaatcggggt gtgtttaaga atattatttg taatttctca
      121 aaagttcctt atagaatttt actgccaaag gaattttatc aacatttatg ttctcgtttt
      181 cgctgtgaac cgacgcatat tgctgaccgt caatgtcaat gggagcaaat ctgattaccg
      241 agtcggaaag tccggtaaat cttctcgcat ccgttccggc aggaagaata aacggaactg
      301 ccgcagcgta gtcaaatacg ctctgcacga ttttcctgac atacttatag ccgtcgcttt
      361 caagagaagt gggcttataa tactcattgt cggagtctct gacgactatc tccacctcgt
      421 atttggcggc tatttccttc agcgcgtcca agtcctttgc caaatcctca tcgttgatac
      481 agcgaagaaa tgcctcgccc ttacagctcc cgtcctcctc ggtatagagc tttctgaacg
      541 tacacccggt tccgagcatt tcgccggccg catcatttag cttgggcatc aattttatca
      601 aaagtcccga aaacagccga agatttgaaa atacgaattt catcggaaat ttcatattag
      661 gtgccatggc ctcaaacatt cctctgacct cggggtggag ctttctgata aacggctttt
      721 tctcatttgc ctccgctata aaagaaacca ttcgcataac gggagtttga tggttgcccg
      781 caaggccgac atgccctttg ctcttctttg ccgtaagctc agctatgcat ctgccctttt
      841 cgtgaaccgc catcatggcg cactttttac ttataccggc catgggaggg tctattaccg
      901 ctccgccctc gtccgatatc caatcaaagg ttatgtgatt tttattgaag taatcgagag
      961 cgagaaccgc tccgtctcct cccgtctctt cattatgaga ggaaaaaaga tatacgttga
     1021 ccgggaactc gaagccctca aaaagcagct cctctattgc agaaaactca gcgaacaaag
     1081 gtgtttttgt atcaactgtt cctctgcccc agagcttgcc gtccttgatt atgcctccga
     1141 acggctcctc ctgccaatca cctgtcgctt caactacgtc atgatgagac ataaccatta
     1201 cattccttgt ctcatcctta ccctttaatt tgtacagata ggcgtcgtcg ccgaaaacgg
     1261 taaactccgc cttttgagtc acaagcggaa agagctctgc aataaccttt ctgagcttta
     1321 aaaactcctc cggcttaaag tcattctttt cggacacggt tttgcacctt atcatctttg
     1381 caaggcgctc tgcgtaaaat tgagccttat cgcccgaaga agcgtttatg ttatcagtca
     1441 caattattct cctttttgtc gcttgccttt nnnnnnnnnn gtggcaagga cctctttttc
     1501 tctgtaacgc aggagcacaa aaatcgcaag gatgcaaaac aggctgcctg caaccgccgt
     1561 aagctttagt ccgagcactc cgacattttc gcctgccgcc gcaccggtta caacaagcga
     1621 gggcactatc attatcgcga gtgaattgcc catcttcaca ataaagctct tcgcagcacc
     1681 gaagattccc tcctggttaa tgccggtcac taccgtatca tactgaatga tatctgccat
     1741 catagcaccc ggcagcacat tgagcacggc aaacgggaat gatacgaaca gcgcgaatac
     1801 gcaagccaaa acgagcttgt tgcccggtat tttatcgctg aagcagataa cgatttcggc
     1861 aatcgagaac acaatacagc ccaatataac gggaagcttc ttccccttcc ttttggcaaa
     1921 tttatttacc agcggataaa gagcaagcga gcctgcaata gatattgcca agataagcac
     1981 cgaatatgtt tcggcaagac ccataagtga ggttacatag tacataatgc aagcctggaa
     2041 aaatgccatt ccggtatttt ccaaaagctg cccgagtgtg acaattctga agtgcttatt
     2101 tgaaaaagcg tgcttcaggg agtcctttaa ggtcacgttg gggattaccg aatgcacgta
     2161 atccttttcc tttatggtaa acgccgggat tataagcagg acaatgccga ttgcggtaaa
     2221 aataccgaag gtcattctcc aggctccgag agtggccata ccggcggatt taaagccgtt
     2281 cacgataagc gggctgacat agccgagcgc gctgccggtt acaaagaaaa acgagcttat
     2341 cgtatatgcg ttaactctca tcgggccgtc ctgcacaagc tcgggaatga gcgcattgtg
     2401 cggaatcata tacaaagtaa agaagaaata atatccccac ataaaagccg caatccaaac
     2461 ggcattaaag ccgcttacgt aattcaacgg cacaaagaac ataagaaacg cgcaaagtcc
     2521 gaacggaaca gccgcctttt gcataagcgg aatacgtctg ccgttt
//
LOCUS       DBMDVO010000125         2559 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19102_length_2559_cov_1.475997, whole genome shotgun sequence.
ACCESSION   DBMDVO010000125 DBMDVO010000000
VERSION     DBMDVO010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19102_length_2559_cov_1.475997"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="ACZPE2_02640"
     CDS             complement(<1..1313)
                     /locus_tag="ACZPE2_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1318777.1"
                     /translation="MKTPIYDFVSDYIAKNGARLHMPGHKGRIFLGAESRDITEICGA
                     DSLFEADGIIAESEKNASNLFDTKRTVYSCEGSTLSIKAMLYLAAINRENKSERPVFV
                     AARNVHRAFVYASAELDADVVWLTSKDNLSLCSCKISAEDLKETLSKQKVKPAAVYIT
                     SPDYLGNTADIAALSQIAHSFGVPLLCDNAHGAYLHFLREKCHPMELGADICCDSAHK
                     TLPVLTGGGYLHFSKNEIKDFSSDAKTAMAVFGSTSPSYLILQSLDLANRYLENGYRE
                     RLFDTVKKVRDVKNFLEENGYRLCGEEPLKITVNTSTFGKSGFELAENLRKNNAECEF
                     CDRDFVVMMVTPETSDGDLEAVKRAFVSHEKTDGKSVAPKTDLPVFVLPEKRLSVRQA
                     VFCSSESMPVENSVGRVAASPAVACPPAIPVVISGEIINESTVEI"
     gene            complement(1326..>2559)
                     /gene="scfB"
                     /locus_tag="ACZPE2_02645"
     CDS             complement(1326..>2559)
                     /gene="scfB"
                     /locus_tag="ACZPE2_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1318778.1"
                     /translation="GDRPDVTRDDVLLCIDDVESLEKNGKLFTEDSFKNLATTFKERS
                     GNVIKALCLHVAHTCNLNCSYCFASQGKYHGDRAVMSFETGKRALDFLIENSGSRRNL
                     EVDFFGGEPLMNWDTVKKLVEYARSIEKEHGKNFRFTLTTNGMLIDDDVIDFANREMH
                     NVVLSLDGRKEIHDKWRVDYAGNGSFDRVVPKFQELVRRRGNKNYYMRGTFTHSNPDF
                     LKDIDAMLELGFTELSMEPVVCAPLDPSALTEEDKPIVMKQYELLAEEMLERKRKGKP
                     FTFYHYMIDLTGGPCIYKRISGCGSGTEYMAVTPWGDLYPCHQFVGEEKFLLGNIWDG
                     VTNTKIQDEFRSCNVYARKECTDCWAKLWCSGGCAANAYHATGKIEGVYDYGCDLFRK
                     RIECAIMLKVAEDAENLK"
BASE COUNT          617 a          662 c          578 g          702 t
ORIGIN      
        1 ataatttcaa ccgtactctc atttataatc tcgccgctta taaccacggg gatagccggc
       61 ggacaggcga cggcaggcga tgcggcaact cttccgacgc tgttttcgac aggcattgat
      121 tcgctgctgc aaaaaaccgc ttgcctcacg gaaagcctct tttcggggag cacaaacacg
      181 ggtaaatccg ttttcggcgc aaccgatttg ccgtccgttt tttcgtgaga gacgaaggct
      241 cttttgacag cttcaagatc tccgtcggaa gtttccggtg ttaccatcat aacgacgaag
      301 tctctgtcgc agaactcaca ttcggcgttg ttttttctga gattttcggc aagctcaaat
      361 ccgctttttc caaaagtcga tgtattgacg gttattttga gcggctcttc tccgcaaaga
      421 cgataaccgt tttcctccaa gaaatttttt acatcgcgca ccttttttac tgtatcaaag
      481 agcctttcgc gataaccgtt ttcaaggtat cggttcgcaa ggtcaagcga ctgcaatatg
      541 agatatgacg gacttgttga gccgaaaacg gccattgccg tcttggcgtc cgacgaaaaa
      601 tccttgattt cattctttga aaagtgcaga tatccgcctc ccgttaaaac gggcagcgtc
      661 ttgtgcgcgg aatcacagca aatgtccgct ccgagctcca tagggtggca tttctcacgt
      721 aaaaagtgaa gatacgcgcc gtgtgcattg tcgcacaaaa gcggcacacc gaaggaatgt
      781 gcaatttgtg agagcgcggc aatatccgcc gtgttgccga gataatcggg acttgtaatg
      841 tatactgcgg caggcttaac tttttgcttt gacagcgttt ctttcaaatc ctccgccgaa
      901 atcttgcagc tgcaaagcga taaattgtct tttgatgtga gccaaacaac atcggcgtca
      961 agctccgccg aagcataaac aaacgctctg tgaacgttgc gtgcggctac aaaaaccggt
     1021 ctttcgcttt tattttcacg gtttatcgcg gcgagataca gcatagcctt aattgagagc
     1081 gttgagccct cacacgaata aacagtcctt tttgtgtcaa acagatttga tgcgtttttt
     1141 tcgctctccg ctatgatacc gtccgcctca aaaagcgaat ctgcaccgca tatttccgtt
     1201 atatcacgcg actctgcacc taaaaatata cggcccttat ggcccggcat atgaagccgc
     1261 gcaccgtttt ttgcaatata atccgaaaca aaatcgtaaa tcggcgtttt cacagcatca
     1321 cctgcttatt taaggttttc ggcgtcctct gccaccttga gcatgattgc gcactctatt
     1381 cttttgcgga agaggtcaca gccgtagtca tatacgccct cgattttacc cgtggcatga
     1441 tatgcgtttg ccgcacatcc gccggagcac cagagcttag cccagcaatc ggtacactct
     1501 tttcgcgcgt atacattgca gcttctgaac tcgtcctgga tttttgtgtt ggtaacgccg
     1561 tcccaaatat taccgagcag gaatttttcc tcgccgacaa actgatgaca cggatataag
     1621 tcgccccacg gagttacagc catatattcc gttcccgaac cgcaacccga gatgcgctta
     1681 tatatacacg ggccgcccgt caaatctatc atatagtgat aaaacgtaaa gggcttacct
     1741 tttctttttc tctcaagcat ttcctctgcg agaagctcgt actgcttcat aacaatcggc
     1801 ttgtcctctt ccgtcagagc cgacgggtct aagggagcgc aaacgacggg ctccatactg
     1861 agctccgtga agccgagctc gagcattgcg tcaatatcct tcaggaaatc gggatttgaa
     1921 tgtgtaaacg tgcccctcat atagtaattc ttgtttccgc ggcggcgtac aagctcttga
     1981 aatttcggca caactctgtc gaagctgccg ttgcctgcat agtcaacgcg ccatttatcg
     2041 tgaatttctt ttctgccgtc gaggctcaaa acgacgttat gcatctcacg gtttgcaaaa
     2101 tctataacgt cgtcgtctat cagcatgccg tttgtcgtaa gagtaaagcg gaaatttttg
     2161 ccgtgctcct tttctatgct gcgagcgtat tcaacgagct ttttaaccgt gtcccaattc
     2221 ataagcggtt ctccgccgaa gaaatcgacc tcaagattgc gtcgcgaacc cgagttttct
     2281 ataaggaagt cgagcgcgcg cttaccggtt tcgaaactca ttacggctct gtcaccgtgg
     2341 tatttaccct ggctcgcaaa gcaatatgag cagttgaggt tgcaggtgtg agcaacgtgc
     2401 agacacagcg ccttaataac attaccggaa cgctccttaa aggtcgtggc aagattttta
     2461 aagctgtcct ctgtgaagag cttgccgttt ttttcaagag attcaacgtc atcgatacaa
     2521 agaagcacat cgtctcttgt gacgtcgggt ctgtcgccg
//
LOCUS       DBMDVO010000126         2559 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19105_length_2559_cov_1.250203, whole genome shotgun sequence.
ACCESSION   DBMDVO010000126 DBMDVO010000000
VERSION     DBMDVO010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19105_length_2559_cov_1.250203"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="ACZPE2_02650"
     CDS             <1..211
                     /locus_tag="ACZPE2_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318779.1"
                     /translation="PVVNVIPQLCYRNLLYTAVTRAKELMIIVGTKDEIVKMAANDKK
                     TRRYSALKKLLLNDAAPAILGTTGI"
     gene            complement(299..2377)
                     /locus_tag="ACZPE2_02655"
     CDS             complement(299..2377)
                     /locus_tag="ACZPE2_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318780.1"
                     /translation="MKRFLAVLLSLILAAGSLFVAAFAADGKKEKVYPVILLQGYSGP
                     QLFNQDTGEKAWGLDFDKVKEHVLNDYGKELANGAKEYAKGNPDPLVDTLGTILLDVM
                     DPIACNADGSSKYNLDTFPKGAEATRMSTLIANGQEEYIGEKPIMTGFVEKLTQQGVE
                     NAADYIFIYTNDWRKGQAQYAKDIDAYIDEVRALTGSDKVDIYGLSFGGQCGASYLYY
                     YGEKAKVHKACLNVPAIGGTNMVGDPLLGNDITLDFPTILQFVEIGFRSENEWEWILE
                     FLSSLTGGYQNLNKIVNLVAQKYIVDYIDKFGSIWDFIPLNVYDEVKARLIRDGYVDP
                     VAAAPLIAASDEFHYNALANMSEGLKRAQKAGTQIAIMSNTGINGVTGTYKNSDYIID
                     VHTSSGSACAPFGEQFPEDYTPVGTQCNNKKHWHISPDRDIDATCSYLPENTWFIKGQ
                     FHGQSNWDSYSREFILEFMFGDSIKDIYSNPKYPQFELAQNPADGLYMRFDNTNSGFH
                     TSEDTALVFTNLSEQYTIDILDISAKGFNLFPEYNSYSGIGAGSTEVISMTDHCFAKS
                     TQPISIKVRYRLNSPQRLIKEKTFTFTHLSDDEIKDYPFINDAAKLIIGENEPAPVTE
                     TAPADTTKNTSENIEERAEVRLSGDENKVSSKIPKTGSAKRGIALSSFAVITAAAAAG
                     VIIKKKREEA"
BASE COUNT          594 a          576 c          609 g          780 t
ORIGIN      
        1 gcccgtcgta aatgtaatac cgcagctctg ttacagaaat ctgctttata ccgccgtaac
       61 aagggcgaag gagcttatga taattgtcgg cacgaaagac gagattgtga aaatggcggc
      121 taacgataaa aaaacacgtc gctattcggc acttaaaaag ctgctgctta atgatgccgc
      181 tcccgcaatt ctcggtacaa cgggtattta aaagagctga accgattttt aagagcaaac
      241 aaaagacacc ccttgggcac tttaagtact caaggggtgc tttttttctt ggattatatc
      301 aggcttcttc tcttttcttt ttgattatca cgcctgccgc ggcagctgcc gtaataacgg
      361 caaatgacga taacgcaata ccgcgttttg cgcttccggt ttttggaatt ttggatgaga
      421 ctttattttc gtcgcctgaa agtcgtactt cggcgcgctc ttcgatattc tccgaagtgt
      481 ttttcgttgt atccgccgga gctgtttcgg ttacgggtgc aggctcgttt tcgccgatta
      541 taagctttgc ggcgtcattt atgaacggat aatccttaat ttcatcgtcg cttaagtgcg
      601 taaaggtaaa tgttttttcc tttatgagcc tttgcggact gttgaggcgg tatctgacct
      661 ttatgctgat gggctgagtg cttttggcaa agcagtggtc ggtcatggaa attacctcgg
      721 tactgccggc gcctattcct gaatatgaat tgtactccgg gaaaaggttg aagcccttag
      781 ccgaaatgtc gagaatatct attgtgtatt gctccgagag atttgtaaat acaagtgccg
      841 tgtcctccga cgtgtggaag ccggagtttg tattgtcaaa tctcatatag agaccgtccg
      901 ccggattctg ggcaagctca aattgcggat atttaggatt tgagtatata tcctttatac
      961 tgtcgccgaa cataaactcg agtataaact cgcgagagta tgagtcccag ttgctttgac
     1021 cgtggaactg cccctttata aaccatgtgt tttccggaag atatgagcag gtggcgtcta
     1081 tgtctctgtc gggagagatg tgccaatgct ttttgttgtt acactgtgtt ccgacgggag
     1141 tgtaatcctc cgggaactgc tctccgaacg gagcgcaggc agagcccgat gaagtgtgca
     1201 cgtctattat atagtcggag tttttgtatg taccggtaac gccgtttatg cccgtatttg
     1261 acattatggc tatctgagtg ccggcttttt gtgctctttt aagaccctca ctcatatttg
     1321 cgagcgcgtt gtagtgaaat tcgtccgagg ctgctatcag cggagcggcc gcgacggggt
     1381 ctacgtatcc gtctcttata agacgggctt tcacttcatc gtatacgtta agagggataa
     1441 agtcccatat actgccgaat ttgtcaatgt aatccacaat gtatttctgc gcgacgagat
     1501 tgactatttt gttgaggttt tggtaaccgc ctgtaagaga tgacaggaac tcgagtatcc
     1561 attcccactc gttttcggag cggaaaccga tttcgacaaa ctgcaatatc gtcgggaagt
     1621 caagcgttat atcgttgccg agcaacgggt cgcctaccat attggttccg cctattgccg
     1681 gaacgttgag acaggccttg tgaacctttg ctttttcgcc gtaataatag agatacgagg
     1741 caccgcattg accgccgaag ctgaggccgt atatatctac cttgtcggaa cccgtcaagg
     1801 ctcttacttc atcgatatat gcgtcaatgt cctttgcgta ttgcgcctga cccttacgcc
     1861 aatcgttggt gtaaatgaat atgtagtcgg cggcattttc aacaccctgt tgtgtgagtt
     1921 tttccacgaa gccggtcatt atgggctttt cgccgatata ctcctcctga ccgtttgcaa
     1981 taagcgtgga cattcgcgtg gcctcggcgc ccttcgggaa ggtatcgagg ttgtatttcg
     2041 atgaaccgtc cgcattgcag gctatcgggt ccatcacgtc gagaagaatc gtgccgagtg
     2101 tgtccacaag cggatcgggg ttgccttttg catattcctt cgcgccgttt gcaagctcct
     2161 tgccgtagtc gtttaaaacg tgctccttga ccttgtcaaa gtcaagaccc caggcttttt
     2221 caccggtgtc ctgattaaag agctgcgggc cgctgtagcc ctgtaagaga atgaccggat
     2281 aaactttttc ttttttgccg tctgccgcaa aggccgccac aaagagcgag cctgctgcca
     2341 gtatcagcga caaaagtacc gctaaaaacc ttttcatttt ttactcctcc aagctttgtg
     2401 tattttatac acaaattatt atttattata acatttttaa ttcaaattgt aaataaaata
     2461 ttttttgctt cgtataatcc ctgtttgtgt taagcaaaat tcggcttttc gctctttttt
     2521 attgacttta atacgctttg gtgttaacat aacaaggat
//
LOCUS       DBMDVO010000127         2548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19247_length_2548_cov_1.282387, whole genome shotgun sequence.
ACCESSION   DBMDVO010000127 DBMDVO010000000
VERSION     DBMDVO010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19247_length_2548_cov_1.282387"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="ACZPE2_02660"
     CDS             complement(<1..427)
                     /locus_tag="ACZPE2_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318781.1"
                     /translation="MSKNRMKKFISILLALTMTVLCFVPAAAAEPKDKVTPILIIAGF
                     SEYALVDGDGNQVWGPSQDAIVEAAKNAIAPLGAFLKGDYETFCTGIVEIANNLFEPV
                     SCNPDGTAKHPDVTVIDQYTEPVSQYGLDEVTRADVFDKD"
     gene            complement(505..1434)
                     /locus_tag="ACZPE2_02665"
     CDS             complement(505..1434)
                     /locus_tag="ACZPE2_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1318782.1"
                     /translation="MKKYYPEGARPDIEAVEKRLSSPAELKNAFLSGESLEARAVLCD
                     RGHNLDVDLRCMKGFMPREECAMGIENNTVRDIAIISRVNKPVDFVITGFSENERGET
                     FALLSRRKLQEECAANYTSRLIPGDIIDARVTHLEPFGAFCDIGAGISALMPIDGISV
                     SRIPHPCVRFSVNQDIKAVVKSIDENGRITLSQKELLGTWEENASKFAPGETVPGVIR
                     SVEDYGIFVELAPNLAGLAECALGPRAGDSAAVYIKSINPERMKIKLIIVDYFTGDGK
                     SPAADYFISDGHIDKWTYSPETSIKRVETDFMS"
     gene            complement(1696..>2548)
                     /locus_tag="ACZPE2_02670"
     CDS             complement(1696..>2548)
                     /locus_tag="ACZPE2_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1318783.1"
                     /translation="NVPIAAYGLPGTGKLRKGVVEAIKRSDSKAVIMAHHGALCMGKD
                     YDEAFKVAAELEKICETTVKNRHRLITGKVAETLGDVAEYIGSLFDSSAKEAPVFEPC
                     NSERDGSVFNISAVDGDGSIVRIDIKTGELVAGNDYPASAEMHRAIYKKRKDVNFIMH
                     TKTPAEVAMSKSGKTMKPLLDDFAQLVGATVRSVTFNPNSTKKTAKKVVKALKGRNGV
                     LLNNNGALCVSGSEYDATAVEMVMDKGCKTAVGARLFEAGKPISPIETRLMRFIYLKK
                     YSKQAKK"
BASE COUNT          579 a          685 c          568 g          716 t
ORIGIN      
        1 tatccttatc gaagacgtct gcccttgtga cctcgtcaag tccgtattgc gataccggct
       61 cggtatattg gtctataacg gtgacgtcgg ggtgctttgc cgtgccgtcg ggattgcacg
      121 aaaccggctc aaaaagattg tttgctattt ccactattcc cgtgcaaaac gtttcataat
      181 ctccctttag aaacgcgccg agcggagcta tggcgttttt cgccgcttca actattgcgt
      241 cctgcgacgg cccccaaacc tgatttccgt cgccgtccac cagcgcatat tcgctgaagc
      301 cggctattat caggataggc gtaaccttat ccttgggctc tgcggctgcc gcgggaacaa
      361 agcaaagaac tgtcatggtc agtgccaaaa gtatactgat aaactttttc atcctgtttt
      421 ttgacataat tattctcccc ctgctttaaa caattaagta gatattacca taattatatc
      481 atttttgcaa tattttttat acaatcagct cataaaatcc gtctcaaccc ttttaattga
      541 cgtttcgggc gaatatgtcc atttatctat gtgaccgtca cttataaaat aatccgccgc
      601 cggacttttg ccgtcaccgg taaaatagtc cacgataata agctttattt tcatcctttc
      661 gggatttatg ctttttatgt aaaccgcggc actgtcgcct gcgcgcggcc ctaaggcgca
      721 ttcggcaagc cctgcaagat tgggcgcaag ctccacgaat ataccgtaat cctccacaga
      781 gcgtattact ccgggcacgg tttctccggg cgcgaatttt gaagcgtttt cctcccaagt
      841 gccaagcagt tccttttgcg acaaggtgat tctgccgttt tcgtctatcg atttcacaac
      901 tgcctttata tcctgattaa ccgaaaaacg aacacacgga tgaggtatgc gcgacaccga
      961 gataccgtct atcggcataa gtgccgaaat gcctgcgcct atgtcgcaaa acgcgccgaa
     1021 cggctcaagg tgcgtcacgc gcgcgtcgat tatatcgccc ggaatgagtc ttgaagtata
     1081 atttgccgcg cactcctctt ggagcttgcg gcggcttagc agagcgaatg tctcgcccct
     1141 ttcattctcg gaaaatcccg ttatcacaaa gtcaacgggc ttattaacgc gcgatattat
     1201 ggctatgtcg cgaaccgtgt tattttcaat ccccatagcg cattcctcgc gcggcataaa
     1261 tcccttcata caccttaaat ccacgtcgag attgtgtccg cggtcgcaga gaaccgcgcg
     1321 cgcctcaagc gattctcccg acaaaaaggc gtttttgagc tccgccgggc ttgaaagtct
     1381 tttttcgacg gcttcaatgt ctgggcgtgc accctcgggg taatattttt tcataattct
     1441 cacctgcttt gtttacacta tgcctgccgc tttataaaca ccgtgtccct tatggcaggt
     1501 acaacactat atgtatatgc ggcagctctt cggttgagaa ttaacgcgcg attttatttt
     1561 agcccggcaa gcctccggtt ttgccgcgac gcagaacgaa gcgagccgct caaaaccgta
     1621 ataaacaagg tccgcaaaat gccaaagtgc cgcaaacacg aggtttgcga cactttttta
     1681 caccatattc aaatatcact ttttagcctg ctttgagtac ttcttgagat atataaatct
     1741 cataagtctt gtctcaatgg ggcttatggg cttgcctgcc tcaaacagtc ttgcgccgac
     1801 tgcggtcttg cagcccttat ccattaccat ttcaaccgct gtggcatcat attcgctgcc
     1861 cgatacgcaa agcgcgccgt tgttgttaag aagaacgccg tttctgccct taagagcctt
     1921 tacgaccttc ttagcggtct ttttggtgct gttgggatta aatgttaccg agcgaacggt
     1981 tgcgccgaca agctgtgcaa aatcgtcaag aagcggcttc attgttttgc ccgacttaga
     2041 cattgcgact tccgccggtg ttttggtgtg cataatgaag tttacgtcct tgcgcttttt
     2101 atagatggct ctgtgcattt cagccgaagc cggataatca ttgcctgcta caagctcacc
     2161 cgttttaata tctattctga ctatcgagcc gtcgccgtct acggcactta tattaaacac
     2221 gctgccgtca cgttcgctgt tgcacggctc aaataccgga gcctccttgg cactcgagtc
     2281 aaagagagag ccgatatact cggctacgtc gccgagggtc tcggctacct taccggtgat
     2341 gagacggtgt ctgtttttaa cggtagtttc gcagatcttt tcgagctctg ccgctacctt
     2401 aaacgcctcg tcgtaatcct tacccatgca aagagcaccg tggtgagcca taattacggc
     2461 cttggagtcc gaacgcttta tagcctcgac aacgcccttg cgaagcttgc cggttccggg
     2521 aagtccgtat gccgcaatcg ggacatta
//
LOCUS       DBMDVO010000128         2545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19281_length_2545_cov_1.130933, whole genome shotgun sequence.
ACCESSION   DBMDVO010000128 DBMDVO010000000
VERSION     DBMDVO010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19281_length_2545_cov_1.130933"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    92..319
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            446..2170
                     /gene="leuA"
                     /locus_tag="ACZPE2_02675"
     CDS             446..2170
                     /gene="leuA"
                     /locus_tag="ACZPE2_02675"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1318784.1"
                     /translation="MKTNKYIPFEPIDIPDRTWPSKTIKEAPVWCSVDLRDGNQALID
                     PMNLEQKLEMFKTLVDIGIKEIEVGFPSASETEYEILRTLIDGNYIPDDVTIQVLVQS
                     REHLIKKTFEAVKGAKNVIIHFYNSTSTLQRKVVFKKDMPGIIDIAVNGARLIKKLSD
                     ELMETSDINIRYEYSPESFTGTEMDNAVEICQRVLEELGATPENKVILNLPSTVEGST
                     PNGYADQIEYFCRRLKDRDSAVISLHPHNDRGTAVAAAELGLLAGAERIEGTLFGNGE
                     RTGNVDIVTLALNMYTQNVDCKLDFHDINHIKEVYEDATNMKVGPRQPYAGELVFTAF
                     SGSHQDAINKGKAFMRENNPDKWEVPYLPIDPADVGREYEPIIRINSQSGKGGAAYIM
                     QEYGFAMPKAMHPEFGAVVQKACDEKGKELRADEVFELFCEEYRNVCGPYKLLNHKFS
                     EEKLESESRTRVHFTGTIKYNGNEPVSIEGVGSGPIEAFFNALSHVGITGYKFKNYAE
                     HAISIGADAKACSYIELEAPDGKAVFGIGISHNINYASIKGILCAINRCQPNVVREPQ
                     QPIFEKKQ"
     gene            2276..>2545
                     /locus_tag="ACZPE2_02680"
     CDS             2276..>2545
                     /locus_tag="ACZPE2_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR1318785.1"
                     /translation="MREHNIVVLKGDGIGPEIVDEAVKVLDAAGKKHGFKMNYDYELM
                     GGCAIDETGEPLPQKTVDACKKADCVFLGAVGGPKWDGVPGNKRPE"
BASE COUNT          725 a          547 c          652 g          621 t
ORIGIN      
        1 gagttaaaaa tcagaaaaaa gataaataac aaacgcttga caaatttttt aactgtaatt
       61 ataatataaa gcacaatcga aaagatcgcc tttaaaaact gtgaaggaaa caagacggtt
      121 accgtgagtt tcagagagct gccgtgtggt gaaaggcagt accaagtatc cgtgtataac
      181 tcgttccgga gtttcatagc tgaagtcata ggctgtgacg gcagacaccg ttatatgtca
      241 ggtcttgttg gagaccgcag agggcagcct cggctgccga attagggtgg tacagcgtgg
      301 tttttacttc gcccctatct tcttcagaga taagggcgat tttttgtttt gtaaagactg
      361 cgctttattt tttatatttt tcgctgtgct gtcagtcttt gcaaagcaag ctatatgatt
      421 ggagtacttc ataggagaaa taattatgaa aacaaacaag tacattcctt ttgaacctat
      481 cgacattccg gacagaactt ggccgtccaa gaccataaaa gaggcgcctg tttggtgctc
      541 tgtggatctc cgcgacggaa atcaggctct tatagacccc atgaaccttg agcagaagct
      601 tgagatgttc aaaacgcttg tagacatcgg cataaaggaa atcgaggtcg gctttccttc
      661 cgcatcggag acggaatatg aaattctccg cacgcttata gacgggaatt atattccgga
      721 cgatgtgaca attcaggtgc ttgtgcagag ccgcgagcat cttatcaaaa agacatttga
      781 ggctgtaaag ggcgcaaaga acgttataat acacttttac aattccacat cgactcttca
      841 gcgcaaggtc gtatttaaaa aggatatgcc gggcattatc gatattgcgg taaacggtgc
      901 aagacttatt aaaaagctgt cggatgagct tatggaaacg tccgacataa acatacgcta
      961 tgaatattcg ccggagagct ttaccggtac cgaaatggat aacgcggttg aaatatgcca
     1021 aagagttttg gaggagctcg gcgcaacgcc cgaaaacaag gtcatactca accttccttc
     1081 aaccgttgag ggcagcacgc cgaacggtta tgccgatcag atagaatatt tctgccgtcg
     1141 ccttaaagac cgtgacagcg ccgtaatcag cctccatcct cacaatgacc gcggtactgc
     1201 ggtcgcagct gccgagctcg gtcttcttgc aggtgccgag cgtatagagg gaactctttt
     1261 cggcaacggt gagagaaccg gcaatgtgga tatagttacg cttgctctca atatgtatac
     1321 gcaaaatgtc gattgcaagc ttgactttca cgacatcaac catattaaag aggtatatga
     1381 ggacgccaca aatatgaagg taggccctcg ccagccgtat gccggtgagc ttgtttttac
     1441 cgccttttca ggctctcatc aggacgccat aaacaagggt aaggcgttta tgcgtgagaa
     1501 taatcccgac aagtgggagg ttccttatct cccaatagac ccggcagatg tcggcaggga
     1561 atacgagccg ataatccgta taaacagtca gtccggcaag ggcggtgccg cgtatattat
     1621 gcaggaatac ggatttgcga tgcctaaggc catgcacccc gaattcggag cggtagttca
     1681 aaaggcctgt gatgaaaagg gtaaggaact cagagccgat gaggtatttg agctgttctg
     1741 tgaagaatat cgcaatgtct gcggacctta taagcttctc aaccataaat tcagcgaaga
     1801 aaagcttgag agtgagtctc gcaccagggt acattttacg ggtaccataa aatataacgg
     1861 aaacgagccg gtatctatcg agggcgtcgg ttccggcccc atagaggcgt tctttaacgc
     1921 tctgtcgcac gtgggaatca cagggtacaa attcaagaac tatgcggagc acgcaatttc
     1981 aatcggcgcg gacgccaagg cctgctccta tatcgagctt gaagctcccg acggtaaggc
     2041 cgttttcggc ataggcattt cacacaacat aaactatgct tcaataaagg gtattttgtg
     2101 tgccataaac cgttgccagc cgaatgttgt gcgcgagccg cagcagccga tatttgagaa
     2161 aaagcaataa gcttttgcgg caaaggcatt ttttctaaac acaattcggc gttttatatg
     2221 ttcgctttat tcggcaaatc gtgccgtgag cggcaggaaa ggatatggaa gtaatatgag
     2281 agaacataat attgtagtat taaagggcga cggtatcggc cccgaaatag tcgacgaggc
     2341 tgtaaaagtg cttgatgcag caggcaaaaa gcacggcttt aagatgaatt atgattatga
     2401 gcttatgggc ggctgtgcca tagacgagac gggtgagccg cttccgcaga aaaccgtaga
     2461 tgcctgcaaa aaggcggatt gtgtgttttt gggagcggta ggaggtccca agtgggacgg
     2521 cgttcccggc aataaacgcc ccgag
//
LOCUS       DBMDVO010000129         2533 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19411_length_2533_cov_1.196546, whole genome shotgun sequence.
ACCESSION   DBMDVO010000129 DBMDVO010000000
VERSION     DBMDVO010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19411_length_2533_cov_1.196546"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="ACZPE2_02685"
     CDS             complement(<1..327)
                     /locus_tag="ACZPE2_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR1318786.1"
                     /translation="MANTRKNEISTINKIRFDPTNPCEDDIDIRDIAHALSMMTRANG
                     HFTEFYSVARHSVNCALEATARGYSKRVALFCLLHDSAESYIGDMTRPLKMHFPLFSE
                     YEKKLQK"
     gene            complement(476..>2533)
                     /locus_tag="ACZPE2_02690"
     CDS             complement(476..>2533)
                     /locus_tag="ACZPE2_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1318787.1"
                     /translation="DIVNDVSKALMGGTANAGTDNKDGGKENESMLSQFGRDLTKLAK
                     DGKIDPVIGRQKEIERVIQILSRRTKNNPCLIGEPGVGKTAIAEGLALKIVSGEVPEL
                     LKDKKIYSLDLTGMVAGTKYRGDFEERIKKVIDEVKNAKDVILFIDEVHTLIGAGSAE
                     GAADAANILKPSLARGELQIIGATTIEEYRKHIEKDAALERRFQPVMVDEPSQEEAIE
                     ILKGIKDKYEAHHKVKITDEAIESAVKLSTRYIGDRYLPDKAIDLIDEAASRVRLRSY
                     TAPSDLKELEDKKKSVEAEKLSAVNAQEFERAAALRDEERKLDKEIKDKKENWHDMAG
                     KSHDEVTPADIADIVSSWTGVPVTQLSTEESDRLLHMEDELHRRIVGQDEAVEAVSRA
                     IRRGRVGLKDPKKPIGSFIFLGPTGVGKTELCKALAAAMFGDENAMIRLDMSEYMEKH
                     TVSRLIGSPPGYVGYDEGGQLTEKVRRKPYSVVLFDEIEKAHPDVFNMLLQILDDGVL
                     TDGQGRRVDFKNCIIIMTSNVGAKLISQKQKAFGFAAGAKELEQNEKEIKDAVMGELR
                     NTFRPEFLNRVDDIIVFQRLTKENIKEIASRLLAVLQKRVEDMGIEVTFSDEAVSKIA
                     DAGFDDVYGARPLKRAIQSRIEDALSEEMLKGNVKKGGKYICNVKDDKFVFDKAEEKT
                     VTE"
BASE COUNT          574 a          643 c          552 g          764 t
ORIGIN      
        1 cttttgcagc tttttttcat attcggaaaa cagcggaaag tgcattttca gaggtcttgt
       61 catatctcct atatagcttt cggcgctgtc gtgcaaaagg caaaaaagcg cgaccctttt
      121 tgaatatccg cgtgccgttg cctcaagcgc acagtttacg gaatgtcttg ccaccgaata
      181 aaactcggtg aaatgaccgt ttgcccttgt catcatagaa agagcgtgcg ctatgtcgcg
      241 tatgtctata tcgtcttcgc acggatttgt cgggtcaaag cgtattttgt ttattgttga
      301 aatttcgttt tttcttgtgt ttgccacaat accacttccg gagtatattt tacacttttg
      361 ccgctccgag tgcaataccg atttacatac aaaaaagctg caattctttt acatctatta
      421 tatgaaaata tagaaagcgg cccgtaaagt catatggctt tacggaccgc tatgcttatt
      481 cggttacggt tttttcctcg gccttgtcaa aaacaaattt gtcatctttg acattgcaga
      541 tatacttgcc gccctttttc acgttgccct tgagcatttc ctcggacaaa gcgtcctcaa
      601 ttctgctctg gatagcacgc ttcagaggtc ttgcaccgta tacatcgtcg aagcctgcgt
      661 cggctatctt gctgacagct tcatcggaga aagtaacctc gatacccata tcctcaacgc
      721 gtttctggag aacagcgaga agtctcgaag cgatttcttt gatgttttcc ttggtaaggc
      781 gctggaatac tattatgtcg tctactctgt taaggaattc gggacggaag gtgttgcgaa
      841 gctcacccat tacagcgtcc ttgatttcct tttcgttctg ctcaagctct tttgcgcctg
      901 cggcaaaacc gaacgccttt tgcttctgcg aaatgagctt agcaccgaca tttgaggtca
      961 ttatgatgat gcaattctta aagtcaacgc gtctgccctg accgtctgtc agaacaccat
     1021 catcaagtat ctgcaaaagc atattgaaca cgtcggggtg tgccttttca atctcgtcga
     1081 aaagtacaac ggagtacggt tttcttctta ccttttcggt aagctgaccg ccctcgtcat
     1141 agccgacata tccggggggc gaacctatga gacgagacac cgtgtgcttc tccatatact
     1201 cagacatatc aagtcttatc atagcgtttt cgtcaccgaa catagcggcg gccaaagcct
     1261 tgcaaagctc tgttttgccg acgcccgtgg ggcctaagaa gatgaacgag cctatgggct
     1321 ttttcgggtc cttaagtccg actctgccgc ggcggatagc tcttgaaaca gcctcgaccg
     1381 cttcatcctg accgactatg cggcgatgaa gctcatcctc catatgaaga agtctgtcac
     1441 tctcttcggt agaaagctga gttacgggaa ctccggtcca agaggatact atatcggcaa
     1501 tgtcggcggg agttacctcg tcatggctct tgcctgccat atcgtgccag ttttctttct
     1561 tgtccttaat ttccttgtcg agcttacgct cctcatcacg aagagctgca gcacgctcaa
     1621 attcctgagc atttacagcc gagagctttt ccgcctcaac gctctttttc ttgtcttcga
     1681 gttctttaag gtccgacgga gccgtatagg aacgaagtct tacccttgaa gccgcttcat
     1741 cgataaggtc tatggcttta tcgggcaaat atctgtcgcc tatatatctc gttgagagtt
     1801 taacggcgga ctcgatagcc tcatcggtta tctttacctt gtgatgagct tcatatttat
     1861 ctttaatgcc cttgagtatt tcaatagctt cctcctgcga gggttcatca accattacag
     1921 gttggaagcg acgctccaaa gcggcatctt tttcgatgtg cttacggtat tcctcaatag
     1981 tggttgcacc gattatctga agctctcctc ttgcgagaga gggctttaag atattggctg
     2041 cgtcagccgc accctcggca ctgcctgcgc ctataagagt gtgaacctca tctatgaaga
     2101 ggataacgtc cttggcgttt ttaacctcgt cgataacctt tttaattctc tcttcaaaat
     2161 cgccgcggta ttttgtacct gcaaccatac cggtaaggtc aagagaatat atctttttgt
     2221 cctttaaaag ctcgggaacc tcacccgaaa caattttaag agcaagcccc tcggcgattg
     2281 ccgttttgcc gacgccgggc tcacctataa ggcaaggatt gtttttggta cgtcttgaga
     2341 gaatctgaat aaccctctct atttctttct gacggccgat tacagggtca atcttaccgt
     2401 cctttgcgag ctttgtaaga tctctgccga actgcgagag catactctcg ttttccttgc
     2461 cgccgtcctt attgtcggtg ccggcgtttg ccgtaccgcc cataagagcc tttgacacat
     2521 catttacaat gtc
//
LOCUS       DBMDVO010000130         2528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19471_length_2528_cov_1.099712, whole genome shotgun sequence.
ACCESSION   DBMDVO010000130 DBMDVO010000000
VERSION     DBMDVO010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19471_length_2528_cov_1.099712"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="ACZPE2_02695"
     CDS             complement(<1..386)
                     /locus_tag="ACZPE2_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1318788.1"
                     /translation="MKIYGIVAEYNPFHNGHKFQIDEIRKKVGDCAIVSVMSGNYVQR
                     AETAVMSKYARAKAALLGGVDLVLELPMPYAISSAERFAYSSVHILNSLGAVTDISFG
                     SECGSISLLQKAVEALYDENVNLKLKA"
     gene            570..>1023
                     /locus_tag="ACZPE2_02700"
     CDS             570..>1023
                     /locus_tag="ACZPE2_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIR1318789.1"
                     /translation="MKILVINAGSSSLKYQLIDMDDESVIAKGICERIGNEGSCISGK
                     IHGKSEKFEKTVVMKNHTEAFNEVKKALTEGEYKVINDISEIAAVGHRIVQGGALFNH
                     SVLVDDDVIKGIESLCDLAPLHNAAHIQGIKASIELFGKDVPQVVVFDN"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1034..1796
                     /locus_tag="ACZPE2_02705"
     CDS             <1034..1796
                     /locus_tag="ACZPE2_02705"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1318790.1"
                     /translation="FHSTMPPEAYMFGLPYEYYEKYALRKYGFHGTSHRFVSARCADV
                     MGEDIKKLKIVTCHLGNGSSITAVKYGKCIDTSMGLTPLDGEIMGTRTGSIDPSAVLY
                     IMKKEGKTPDEMSNLLNKKSGILGVSGLSSDDRDVKVAAAEGNKRAKLARDMQIYEIK
                     KYIGSYTAAMDGVDAIVFTGGIGENADDMRATVCDGLTFLGIKIDKEFNKTLVKGKEG
                     EISTPDSKVRVFVLPTNEELVIARDTKEIVTNLKK"
     gene            complement(1871..>2528)
                     /locus_tag="ACZPE2_02710"
     CDS             complement(1871..>2528)
                     /locus_tag="ACZPE2_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1318791.1"
                     /translation="RGFTSSRFSFNVKGGRCEACQGDGIVKIEMHFLADVYVPCEICK
                     GARYNHETLEVKYKGKSIYDVLEMTVEEGLEFFRNMPKIARKLQTLYDVGLGYIKLGQ
                     PATTLSGGEAQRVKLATELSKRPTGRTIYILDEPTTGLHTADVHKLIDVLQKLVDAGN
                     TVVVIEHNLDVIKVADHIIDLGPEGGDNGGKIVVCGTPEKVAACKKSYTGMYLKKILK
                     "
BASE COUNT          733 a          563 c          531 g          691 t
ORIGIN      
        1 gctttcagct ttaaattgac attttcatcg tataaagcct caacggcttt ttgtaaaagg
       61 cttatactgc cgcattcact gccgaatgaa atatcagtaa ccgctccgag ggaatttaaa
      121 atatgcacgg aagaataggc aaaacgctca gcagatgata tggcatacgg catcggaagc
      181 tcaagcacaa ggtcaactcc gccgagtagg gcggcttttg ccctcgcgta ctttgacatc
      241 acagccgttt cggctctctg aacgtaatta ccgctcatca ccgaaactat cgcgcagtcc
      301 ccaacctttt tgcgtatttc gtctatctgg aatttatgtc cgttatggaa cggattatac
      361 tctgccacaa taccgtaaat cttcatagat aatctccgtc aaatcaagta ctgtaccggt
      421 caagccggca aaacttcaaa ttaagcatat tttataatat cggcgtaatt tagtcaaatc
      481 tcttcggcat atattatttc ttgaaaaatc tgtcgaaaca gtatataata tcttagataa
      541 ctttattagc tatttgggag gtattatttt tgaaaattct tgttatcaat gcaggcagtt
      601 catcgcttaa atatcagctt atcgatatgg acgacgagag tgttattgca aaaggcatct
      661 gcgaaagaat aggcaatgag ggatcttgca tcagcggtaa gattcacggt aaatcggaaa
      721 agtttgaaaa gaccgttgta atgaaaaatc acaccgaagc tttcaacgaa gttaagaaag
      781 ctcttaccga aggtgaatac aaggttataa acgacatttc ggaaatagct gctgtcggcc
      841 acagaatagt acagggcggt gctcttttca accacagcgt tttagtagat gacgacgtta
      901 ttaagggcat tgagagtctt tgcgaccttg ctccgcttca caacgcagcg cacattcagg
      961 gtattaaagc aagtatcgag ctgttcggta aagatgttcc gcaggtagtt gtgtttgata
     1021 acgnnnnnnn nnnattccac agcaccatgc cgccggaagc atatatgttc ggcctcccct
     1081 atgaatatta tgaaaaatac gcactcagaa aatacggctt ccacggcact tcgcaccgtt
     1141 ttgtaagtgc tcgctgcgca gatgttatgg gcgaagatat caaaaagctt aaaatcgtta
     1201 catgccatct cggaaacggt tcttcgatta cggctgttaa atacggtaaa tgcatagata
     1261 catctatggg tcttacccca ctcgacggcg aaataatggg aacaagaaca ggcagcatag
     1321 acccgtctgc cgttctttac attatgaaaa aagagggtaa gactcccgat gaaatgagca
     1381 accttctcaa caaaaagtcc ggtattctcg gtgtatcggg tctctcttcc gatgacagag
     1441 acgttaaagt agctgccgcc gagggcaaca agagagccaa gctcgcaaga gatatgcaga
     1501 tatacgaaat aaagaaatat atcggttctt acaccgctgc catggatggt gttgacgcaa
     1561 tagttttcac aggcggtatt ggtgaaaatg ccgacgatat gagagctacc gtatgtgacg
     1621 gtctcacctt cctcggtatc aaaatcgaca aagaatttaa caagactctt gtaaagggca
     1681 aagagggaga aatttccaca cccgactcaa aggttcgcgt tttcgttctt ccgacaaacg
     1741 aggagcttgt tatagcaaga gacacaaaag agattgttac aaatcttaaa aaataatctt
     1801 cttaaataga ttgcagttat tcattatatt tcaagaagca cctcaggctt atacttgagg
     1861 tgcttcattt ttatttaagt atctttttaa gatacattcc ggtatacgat tttttgcagg
     1921 cggcaacctt ttccggcgtt ccgcaaacaa caattttgcc gccgttatcg ccgccctccg
     1981 gtcctaaatc tataatatgg tcggcaacct ttatcacatc gagattatgc tcgattacaa
     2041 caaccgtatt acctgcgtca acaagcttct ggagaacgtc aatcagcttg tgaacatcgg
     2101 ctgtgtgaag tccggtggta ggctcgtcga gaatatatat agttctgccg gtcggccttt
     2161 ttgaaagttc ggtagcaagc ttcacgcgct gtgcttcacc gcctgaaaga gtggttgcag
     2221 gctgaccgag cttaatatat cccaaaccga catcatacag ggtttgcagt ttacgcgcaa
     2281 ttttcggcat attacggaag aactcaaggc cctcctcaac cgtcatctcg aggacatcat
     2341 atatggattt acccttgtac tttacctcga gagtttcatg gttgtatctt gcgcctttgc
     2401 atatttcaca cggcacgtac acatccgcca aaaaatgcat ttcgattttt actatgccgt
     2461 cgccctgaca cgcctcacat ctgccgcctt ttacattgaa agaaaatctg ctcgacgtaa
     2521 atccgcgg
//
LOCUS       DBMDVO010000131         2526 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19490_length_2526_cov_0.989278, whole genome shotgun sequence.
ACCESSION   DBMDVO010000131 DBMDVO010000000
VERSION     DBMDVO010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19490_length_2526_cov_0.989278"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="ACZPE2_02715"
     CDS             <1..377
                     /locus_tag="ACZPE2_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1318792.1"
                     /translation="ESFSTADMTDGKPLRVISTNITDESSKYLTFIQGDKPLIKMVSS
                     CGSGKKVLLIKESYGNALAPFLLDTFSEVYVLDPRQESIQGMNIPSFVENNGIDTVLF
                     CNYTMVPSNSKYMNALNAMIGA"
     gene            447..1343
                     /locus_tag="ACZPE2_02720"
     CDS             447..1343
                     /locus_tag="ACZPE2_02720"
                     /inference="COORDINATES: protein motif:HMM:NF049338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7674 family protein"
                     /protein_id="HIR1318793.1"
                     /translation="MMDLNNAVSVLMKGVDSAVTEAGFTLVRPENIEKDALPITVDKE
                     TEKTFIDYAGDNGKIRIQIDGVKLSLLFSEDDGEYKSASENYFDPKDFDERDVKSLCN
                     ELNETILQKYGKNRTAGGKAKKIPVPVSKTAVKNGTSSYDGNTLANRLSALYPDLKPY
                     YKENFEEYGEFLPDTFFIEHANSYIMNTIRLGIKQDMTKLFRILNDIYENGTNDTQSL
                     VAVTILGEMNNDPVMLENANAYMCDDMRDTVILINKFLASGSSKKLREKLKNPPPYKP
                     KKKKSGGIMSQLMGAGGQMPQQ"
     gene            complement(1409..>2526)
                     /locus_tag="ACZPE2_02725"
     CDS             complement(1409..>2526)
                     /locus_tag="ACZPE2_02725"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1318794.1"
                     /translation="AYKWLDVKEYLIGRMTGEFVMTQDSAFASMLYDIHKKCWSEEMC
                     KIYGINPKHLAKIVKCTDKVGELSKKAADELELEPGTAVFGGGGDASLIGVGAGSVDL
                     GDTHVYSGTSGWVSTVVDKSVVDTSAMIAAIVGARDGYYNYFAELETAAKCVEWVKDH
                     LALDEIDIYLDKSKNLKHGKADQEVIYTNLYDYMMAVVNTVPAGSNGVVFTPWLHGNR
                     CPFEDPNSRGMFFNISLETGKSEMIRAVVEGVCFHMRWLLETEAKKVSTSSTIRFVGG
                     GALNDVTCQILADCTGRTVETVDSPQNIGAVGAAVTIAVGTGLIKDIGEAKKLIPAAK
                     VFKPNPALKPVYDRNYKVYTNLYKANKENFAILNGDN"
BASE COUNT          662 a          605 c          609 g          650 t
ORIGIN      
        1 ccgagtcgtt ttccaccgcg gatatgactg acggcaagcc cctgcgcgtt atatcgacaa
       61 atatcaccga tgagtcaagc aaatacctta cgtttataca gggcgataag ccgctcatca
      121 aaatggtgtc gtcctgcggc agcggcaaaa aggtgctttt gataaaggaa tcgtacggca
      181 atgcgctggc accgttttta ctcgacacct tcagcgaggt ctatgttctt gacccgaggc
      241 aggagagcat acagggtatg aatatcccat cgtttgtgga gaataacggc atagatacgg
      301 tgctcttttg caactatacc atggtgccgt caaattcaaa atatatgaac gcactcaacg
      361 caatgatagg cgcttaaaaa tttttaatga caaagtgccc gtcttatggt acaattaact
      421 atataaattt taggcaggta tatgaaatga tggatttaaa caatgctgta tccgttctta
      481 tgaagggtgt tgacagtgct gttaccgagg cgggctttac gcttgtccgt cccgaaaata
      541 ttgaaaagga tgcgctcccg ataaccgtcg ataaggagac cgaaaaaacg tttatcgact
      601 atgcgggtga taacggtaaa atcagaattc agatagacgg cgttaaattg agtctcctct
      661 tttcggagga cgacggagag tataagagcg cgtcggaaaa ctattttgac ccgaaggact
      721 ttgatgagcg cgacgttaaa tcgctttgca acgagcttaa cgagacaatt ttgcagaagt
      781 acggcaaaaa cagaaccgcc ggcggcaagg ccaagaaaat acccgttccg gtttcgaaaa
      841 ccgctgttaa aaacggaact tcgtcttatg acggcaacac tctcgcaaac cgcctttccg
      901 ctctgtaccc cgacctcaag ccgtattaca aggaaaattt tgaggagtac ggcgagtttc
      961 tgcccgacac cttctttatc gagcacgcta attcctatat tatgaacacc attcgtctcg
     1021 gcataaagca ggatatgaca aaacttttca gaatacttaa cgacatatat gaaaacggca
     1081 caaatgacac acagagcctt gttgccgtga cgattctcgg cgagatgaat aacgacccgg
     1141 ttatgctcga gaatgcaaac gcatatatgt gcgacgatat gagagatacg gttattctca
     1201 taaataagtt tttggcctcc ggcagcagca aaaagctccg tgaaaagctc aaaaatccgc
     1261 ctccgtacaa gcctaaaaag aaaaagagcg gcggtattat gtcgcagctt atgggcgcag
     1321 gcggtcagat gccgcagcag taatcgcagt ttaaatttaa tcaaacaaaa aaggcactct
     1381 ttcgagtgcc ttttctttat gcggtttgtt aattgtctcc gttgaggatt gcaaagtttt
     1441 ccttgttcgc cttatagaga tttgtgtaaa ccttgtagtt tctgtcgtaa acgggcttga
     1501 gtgccgggtt aggtttaaag accttagccg cagggataag ctttttcgct tcgccgatgt
     1561 cctttataag acccgtgccg acggctattg ttaccgcggc gccgacagcg cctatattct
     1621 gcggactgtc aacggtttca acggttctgc ccgtgcagtc tgcgagtatc tggcaggtga
     1681 catcgttaag agctccgccg cctacaaaac gaatagtgct tgaggtgctg acctttttag
     1741 cctctgtctc aaggagccag cgcatatgga agcaaacgcc ctccacaaca gctcttatca
     1801 tttcggattt gccggtttca aggctgatgt taaagaacat accgcgcgaa ttcgggtcct
     1861 cgaaggggca gcggttgccg tggagccacg gagtaaacac aacgccgttt gagcctgcgg
     1921 gtaccgtgtt tacgactgcc atcatataat cgtaaaggtt tgtgtaaata acctcctgat
     1981 ctgccttgcc gtgctttaaa tttttgctct tgtcgaggta tatgtcgatt tcgtcgagag
     2041 ccaggtggtc ctttacccac tcaacgcact ttgcggcagt ctcaagctct gcaaaataat
     2101 tgtagtagcc gtcgcgtgcg ccgactattg cggctatcat ggcagaggtg tctacaacgc
     2161 tcttgtcaac aacggttgat acccagccag atgtgccgga ataaacgtgc gtgtcgccga
     2221 ggtcaaccga gcctgcgccg acgcctataa gcgaagcgtc gccgccgccg ccgaataccg
     2281 cggtgccggg ctcgagctca agctcgtcgg cagctttctt tgaaagctcg cctaccttat
     2341 cggtgcattt tactatcttt gcgaggtgct tcggatttat gccgtagatt ttgcacattt
     2401 cctcgctcca gcatttctta tgtatgtcat agagcattga tgcaaaggcg gagtcctgag
     2461 tcataacaaa ctcgcccgtc atacggccta tgaggtattc cttaacgtca agccacttgt
     2521 aagctc
//
LOCUS       DBMDVO010000132         2518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19566_length_2518_cov_1.021928, whole genome shotgun sequence.
ACCESSION   DBMDVO010000132 DBMDVO010000000
VERSION     DBMDVO010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19566_length_2518_cov_1.021928"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            106..318
                     /locus_tag="ACZPE2_02730"
     CDS             106..318
                     /locus_tag="ACZPE2_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318795.1"
                     /translation="MQNDNIDEIIDGIKSQKSKKGAEKFLKNKLSPEQSKKLSDVLSD
                     ENALKNLLSSDRAKELFKKLGGKDAE"
     gene            342..704
                     /locus_tag="ACZPE2_02735"
     CDS             342..704
                     /locus_tag="ACZPE2_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318796.1"
                     /translation="MDNIGDLLSSISPEDMERLKNVASSLMSQSGSEPSSAQKEQGAG
                     GNRNAPNTDISSLFGGDMTKTLMTVATQMNKEDDNTRFISALRPLLSEDRRKKADEAM
                     KFLKLMETLPLLKGFFNV"
     gene            701..1108
                     /locus_tag="ACZPE2_02740"
     CDS             701..1108
                     /locus_tag="ACZPE2_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318797.1"
                     /translation="MKDYSYSELKEMQDRAMERVRAMQQRASETVSRENSLQEEKPKP
                     DAHTEQNSYTKPHHIKMPVNMPPRESTAESRVSKASTDSNPASNSASANENSIIESIL
                     NDPDRAMLLPLLLLLKSEGANEELLMSLLYIMS"
     gene            complement(1176..2111)
                     /gene="cysK"
                     /locus_tag="ACZPE2_02745"
     CDS             complement(1176..2111)
                     /gene="cysK"
                     /locus_tag="ACZPE2_02745"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1318798.1"
                     /translation="MSKIYKSLTDLIGNTPLLELSNYEKENNLEAKILVKLEYFNPAG
                     SVKDRIAKKILDDAEAKGLINKDTTIIEPTSGNTGIGLSSVAAARGYKIIITMPETMS
                     VERRLLMAAYGAKLVLTDGAKGMKGAIAKANELHEQTPNSFIPGQFVNPANPQAHFET
                     TGPEIWNDTDGKVDIFVAGVGTGGTLSGTGKFLKSKNPDIKVVAVEPATSPVLSEGKS
                     GPHKIQGIGAGFVPDTLDTEIYDEIITVENDDAFAYSRAITKSDGILVGISSGAAVKA
                     ATDLARRPENKGKTIVAILPDTGERYLSTQLFKED"
     gene            complement(2144..2299)
                     /locus_tag="ACZPE2_02750"
     CDS             complement(2144..2299)
                     /locus_tag="ACZPE2_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR1318799.1"
                     /translation="MADAKTEKQKVQEITEKLEQGIRPNTIRLSIGTEHIDDIIADLD
                     EAFKAVK"
BASE COUNT          717 a          572 c          553 g          676 t
ORIGIN      
        1 gcgagatttt gtcacggtgc gaatcctaaa gcacaagtat tctcattcat aaaacccccg
       61 tgaaattcat actatacagt ataccaaaat acggaggtaa aatcaatgca gaacgacaac
      121 atcgacgaga taatagacgg tattaaaagc caaaaatcaa aaaaaggcgc ggaaaaattt
      181 ttaaagaaca agctgtcgcc cgagcaatcg aaaaagctca gcgacgtttt aagcgatgaa
      241 aacgccttaa aaaatctgct gtcaagcgac agagccaagg agctgtttaa aaagctcggc
      301 ggcaaagacg cggaataaaa cataccgaaa ggacaattct tatggataat ataggcgacc
      361 tgctgtcctc aataagtccg gaggatatgg agagacttaa aaatgtcgcg tcaagcctaa
      421 tgtcgcaaag cggctccgag ccgtcatccg ctcaaaaaga gcaaggtgca ggcggcaacc
      481 gaaatgcgcc taacaccgat atatcgtcac tcttcggcgg cgatatgacc aaaaccctga
      541 tgaccgtagc cacacagatg aataaagagg acgacaacac aagattcatc tccgctcttc
      601 gccctctgct gagcgaggac aggcgcaaaa aagcagatga agccatgaaa tttttaaagc
      661 tgatggaaac gcttccgttg ttaaaggggt tttttaacgt atgaaagatt acagctattc
      721 tgagcttaag gagatgcagg accgagccat ggagagagtt cgcgccatgc agcagcgtgc
      781 aagcgaaacc gtctcaagag aaaactcgtt acaagaagag aagccaaagc ccgacgcgca
      841 caccgagcaa aactcataca cgaagccgca tcatataaaa atgccggtaa atatgccacc
      901 gcgcgagagc acagcagaaa gccgcgtaag caaagcaagc accgacagca atccggcttc
      961 aaattccgcc tcggcaaacg aaaacagcat tattgaaagc attttgaacg accccgacag
     1021 agccatgctg cttccgcttt tgctcctttt aaaatccgag ggagcaaacg aggagctgct
     1081 tatgtcgcta ctctacataa tgtcataaaa gcggtcaaaa aaggctgtaa aagctctcgc
     1141 ttttacagcc acttgaatta gatgtattcg gttaattaat cctctttaaa caactgagtg
     1201 gagaggtaac gctcgccggt atccggtaaa atcgcaacta ttgtcttacc cttgttttcg
     1261 ggacggcgtg cgagatcggt ggcagcctta acggcagcac ccgaagaaat acccacaaga
     1321 attccgtcgg attttgtaat tgcacggctg taagcgaatg cgtcgtcgtt ttcaaccgtg
     1381 ataatttcgt cataaatctc agtgtcgagc gtatcaggta caaagcccgc gccgataccc
     1441 tgaatcttat gcggacccga tttgccctcg gaaagtacgg gcgaggttgc aggctcaacc
     1501 gcaactacct taatgtcggg atttttgctc tttaagaatt tgcccgtgcc ggaaagtgtt
     1561 ccgcccgtgc ctacgccggc gacaaaaatg tcaaccttac cgtccgtatc gttccagatt
     1621 tcgggaccgg tagtctcaaa atgagcctga ggatttgccg gatttacaaa ctgaccggga
     1681 ataaagctgt tgggagtctg ctcatgaagc tcatttgctt ttgcgatagc acccttcatg
     1741 ccctttgcac cgtcggtaag gacaagctta gcaccgtaag ccgccatcaa aagtctgcgc
     1801 tcaacgctca tggtttcggg cattgtgatt attatcttat agcctcttgc cgccgcaacg
     1861 gagctgagac ctattccggt gttgccgctt gtaggctcaa taatggttgt gtctttattt
     1921 ataaggcctt tagcttctgc gtcatcgaga attttcttcg ctattctgtc tttgacgctg
     1981 cctgccgggt taaagtattc gagctttaca agtattttcg cttcaagatt gttttccttt
     2041 tcataatttg acagttcgag aagcggtgta tttcctataa gatccgtgag gcttttatat
     2101 atttttgaca taatgattat ctcctttaat tccgttattt aaatcattta acggctttga
     2161 acgcttcgtc aaggtcggct atgatatcgt ctatatgctc tgtgcctatt gataggcgga
     2221 tagtgttcgg gcgaataccc tgctccagct tctccgttat ttcctgtact ttctgttttt
     2281 ctgttttagc gtctgccatt gtttttgttt cttaattgtc tgtttctatt ctattaaatt
     2341 tctccctttt cggaaaagtg tttctgatat tacttaaccc aaactatcct gttatttata
     2401 cattttaaga agttaatgca atttgagcta ttgatttgct tggatatgat aaagattagt
     2461 tttgctgact ggattgaaat tgagtttagg tttaaaacat aataaaagct atccaatt
//
LOCUS       DBMDVO010000133         2517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19576_length_2517_cov_1.210679, whole genome shotgun sequence.
ACCESSION   DBMDVO010000133 DBMDVO010000000
VERSION     DBMDVO010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19576_length_2517_cov_1.210679"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="ACZPE2_02755"
     CDS             complement(<1..521)
                     /locus_tag="ACZPE2_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318800.1"
                     /translation="MSEKHSSASKSKLLGTVLLILALFGFFVFIYRLCYYHYEYDPQY
                     SPVDYGKYNILSYFTVQSNFFAYVYFLCAALSIFGVKKAEKIGFNPYIGALVTVYVLV
                     AGITYCAGIPMGLTPPFKWDTPAHSMSSFIQVYYHMIMPPAALIVWFFPFTDRAIGKK
                     CFWLSGIYPLVYS"
     assembly_gap    663..672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<673..1663)
                     /locus_tag="ACZPE2_02760"
     CDS             complement(<673..1663)
                     /locus_tag="ACZPE2_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR1318801.1"
                     /translation="MVPAKAKKVIKVILIILLAAVLIVGGYVAYVMIDYHRIEDNQAL
                     QINDATSDAALVDTEYKVISYNIGFAAYTPDFGFFMDGGTQSRAESEESVKNVISGVG
                     DFLKSEAPDFILIEEVDKNATRSYHYDEEAALRNTLEGYDSTFTVNFDSPYLFYPLSK
                     PHGKSYAGMLTLSRFNIESGLRRSLPIEDGFMKFVDLDRCYSVSRVSVSNDKELVLYT
                     LHLSAYTSDGTIATEQLTMLINDMQAEYEKGNYCIAGGDFNKDLLVDSGKIFGIDGAD
                     YTWAQPVDPTLFDGTNLSMVAPFNEEKPVPSCRNADGPYNDNQFVLTVDGFIVS"
     gene            complement(2293..2383)
                     /locus_tag="ACZPE2_02765"
     tRNA            complement(2293..2383)
                     /locus_tag="ACZPE2_02765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2347..2349),aa:Ser,seq:gct)
BASE COUNT          727 a          579 c          543 g          658 t
ORIGIN      
        1 atcgaataaa caagcggata aattcccgac agccaaaagc attttttgcc tattgcacgg
       61 tcggtaaacg ggaaaaacca tactataagc gccgccggcg gcattatcat atggtaatac
      121 acctgtataa agctcgacat cgaatgagcc ggcgtatccc acttaaaggg cggagtaagc
      181 cccattggaa taccggcgca atatgtaatt ccggccacga gaacatatac ggtaacaaga
      241 gcaccaatat aaggattaaa gcctattttc tccgcctttt taactccgaa tatcgaaagt
      301 gccgcgcaca ggaaatacac atacgcaaag aaatttgact gcacggtaaa gtaagacagg
      361 atattgtatt tgccgtaatc caccgggctg tattgtgggt cgtactcgta gtgatagtaa
      421 caaagtcgat agataaacac aaagaacccg aagagagcca aaatcaaaag caccgtgccg
      481 agtaatttac tctttgacgc cgagctgtgt ttttcagaca taaggaatgc ccctctcttt
      541 attctgcgac cgaaaatctc aaattcaccc attaagctta aacgtcatat ataccggatt
      601 gtgatcggaa tacttaaatc cgagatcgta gacatcgctg ccggaaacgg taacattgtc
      661 tgnnnnnnnn nnctgagact ataaagccgt caacagtaag gacaaattga ttgtcgttat
      721 acggaccgtc cgcatttctg catgacggaa cgggtttttc ctcgttaaac ggagctacca
      781 tactgagatt ggtaccgtca aacagcgtcg gatctaccgg ctgtgcccaa gtatagtcgg
      841 caccgtcgat tccgaatatt tttccggagt cgacgagcaa atccttatta aagtcgccgc
      901 ctgcgataca ataattaccc ttttcatact cagcctgcat atcatttatg agcatggtta
      961 gctgttcggt ggcgattgtg ccgtcggaag tgtatgcgga aagatgaagc gtatatagca
     1021 caagctcctt atcgttgctg acggaaactc ttgataccga atagcaacgg tcaaggtcaa
     1081 caaatttcat aaatccgtct tcgataggca gactgcgtct gagtccgctt tcaatattga
     1141 atcttgataa tgtaagcata ccggcatacg attttccatg cggcttgctg agcggataga
     1201 atagatacgg gctgtcaaaa ttcacggtaa atgtagagtc ataaccctca agcgtatttc
     1261 tgagagcagc ctcttcatca tagtgatagc tgcgagtcgc atttttatcg acctcttcaa
     1321 taaggatgaa atcgggagct tctgatttaa gaaaatcccc gacgccgctt atcacatttt
     1381 tgaccgactc ctccgattcg gcacggcttt gtgtaccgcc gtccataaag aagccgaaat
     1441 cgggcgtata tgccgcgaaa ccgatattgt aggatatgac cttatattcc gtatctacaa
     1501 gagcagcatc actcgttgca tcgttaattt gcaaggcttg attatcttca attctgtgat
     1561 aatcaatcat aacataggcg acatatcccc ccactatcag aaccgccgca agtaaaataa
     1621 ttaaaatgac cttaataacc ttttttgcct tagctggcac cacaagcacc tctttcttgt
     1681 tattttgtag aaaaattaaa agtagactgc tttgaattat atcactattt cacattatat
     1741 tcaagacaaa taacaaaaca ggcaagcaaa gcgggctttt ttatacataa tatccgtttg
     1801 tgtgcagaaa acgtcaacaa tcgtgaaaaa aggctctctc ccgtcaggat ctggctgtga
     1861 gcaacgcgaa cagactgagg gcttaaatta ggctcccttg taaaggagct gtcatcgaag
     1921 atgactgagg gattaaaaag cgaagcactt tctttttgaa atctgttttt tgagaaaatc
     1981 cctccggctt gccttaacga ggcccacctc cctttacaag ggaggctgtt ttccgcaaaa
     2041 agatggacaa agagttcgag tctcctgccc tttttgcaat atacagaaaa aaggtaccgg
     2101 ggaaaatccc gatacctttc ctggcgcaga aggagagact cgacgggttt tcaacccgtc
     2161 gtgttcgctt tgcgaacagt ttattttatg tccctttttg ctccgcaaaa agatggacga
     2221 agagttcgag tctctctgcc ctttttgtat tatacagaaa aaaggtaccg gggaaaatcc
     2281 cgataccttt tctggcgcag aaggagagac tcgaactctc gcgccggtta cccgacctac
     2341 gcccttagca ggggcgcctc gtcaccaact tgagtacttc tgcattggta gcgtctatga
     2401 cactattcga ttgttaaaag agagagcgga aaatccgctt tctcttctaa gtggcggaga
     2461 gagtgggatt cgaacccacg gtacgttgcc gtacgacggt tttcaagacc gttccgt
//
LOCUS       DBMDVO010000134         2506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19695_length_2506_cov_1.661123, whole genome shotgun sequence.
ACCESSION   DBMDVO010000134 DBMDVO010000000
VERSION     DBMDVO010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19695_length_2506_cov_1.661123"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="ACZPE2_02770"
     CDS             complement(<1..1572)
                     /locus_tag="ACZPE2_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1318802.1"
                     /translation="MVYRIYVEKKKQFADEAASLLSDIRSLLMIDSLEELRIFNRYDV
                     ENISEELFERCEKTVFSEPQLDNTYARLPQTDATVFAVEYLPGQFDQRADSCAQCIQI
                     VSEGDKPTVRTAKVYMLFGKLAPAQLEAIKKYVINPVESREASLEKPETLNVNYNIPT
                     SVETLTGFTELNENELKAFIDKYALAMDLADIEFCRKYFKGEHRDPTITEIRMIDTYW
                     SDHCRHTTFLTTIDGVKTEDKTIQKAYDRYVSIRKELGRTKPQNLMDLATIGARYLKH
                     TGQLKNLDESDEINACTVKIKVDVDGEDEDWLFLFKNETHNHPTEIEPFGGAATCIGG
                     AIRDPLSGRGYVYQAMRVTGAADPLKPVSETMPGKLPQRKLVTTAAAGYSSYGNQIGL
                     ATGSVDEIYHPGYVAKRLEIGAVVGAVPAKNVRREEPVPGDIIILLGGRTGRDGCGGA
                     TGSSKSHKLSSLEHCGAEVQKGNAPEERKLQRLFRNSEVTKLIKRCNDFGAGGVSVAI
                     GELADGLHIDLNKVPK"
     gene            complement(1588..>2506)
                     /gene="purD"
                     /locus_tag="ACZPE2_02775"
     CDS             complement(1588..>2506)
                     /gene="purD"
                     /locus_tag="ACZPE2_02775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1318803.1"
                     /translation="VFSDPEEALSYIKEKNEYPTVIKADGLALGKGVIIAESEEQAED
                     AVKTIMEDKKFGDSGNNVVIEEFLTGPEVSVLSFTDGKTVVPMVSSMDHKRALDGDKG
                     LNTGGMGTVAPNPYYTAEIAKECAEKIFLPTVKAMNNENRTFKGCLYFGLMLTPKGPK
                     VIEYNCRFGDPETQVVLPLLKTDLLTIMRAVHDETLSFLNVEFKDESAACVVIASGGY
                     PLSYGKGYEIDFGDSENTENVTVFHAGTAEKDGKIVTSGGRVLGVTATGKDLNAALST
                     AYKATEQIKFKDAFYRKDIGARALAALKK"
BASE COUNT          543 a          638 c          602 g          723 t
ORIGIN      
        1 cttggggact ttattcaaat caatgtgcaa gccgtcggca agctcgccta tggctaccga
       61 tacgccgccc gcaccgaagt cgttacatct ttttataagc tttgtaactt cactgtttct
      121 gaaaagtctc tgcaattttc gctcttccgg agcgttaccc ttttgaacct cggcaccgca
      181 atgctcgagt gacgagagct tatgcgattt tgaagaacct gtcgcaccgc cgcagccgtc
      241 acggccggtt ctgccgccga gtaagatgat tatgtcaccg ggaacaggct cttcacggcg
      301 aacatttttt gcgggaacgg cacctacaac ggcacctatt tcaagacgct ttgcaacata
      361 tccgggatgg tagatttcgt ctaccgaacc ggtcgcaagc cctatctggt tgccgtatga
      421 gctgtagcct gccgcagctg tggttacgag ctttctctgc ggcagcttac cgggcatggt
      481 ttcgcttacg ggctttaacg ggtccgccgc accggtaaca cgcattgcct gataaacata
      541 tcctctgccc gacagcgggt cgcggatagc tccgcctatg caggttgccg cgccgccgaa
      601 cggctcgatt tccgtcgggt ggttgtgtgt ttcgttttta aataagaaga gccaatcttc
      661 gtcttcgccg tctacgtcaa cctttatttt tacggtgcag gcattgattt catccgattc
      721 atcaagattt ttgagctgac ccgtgtgctt taaataacgc gctccgatag tcgccaaatc
      781 cataaggttc tgcggctttg ttctgccaag ctccttgcgt atggaaacat atctgtcgta
      841 tgccttttgt atggttttgt cctctgtttt tacgccgtct atcgtcgtga gaaacgtggt
      901 atggcggcaa tggtccgacc agtatgtatc aatcattctg atttccgtta tagtcgggtc
      961 gcgatgctcg cctttgaagt atttgcggca aaattcaata tccgcaaggt ccattgcaag
     1021 agcatatttg tctataaatg ccttgagctc attttcgtta agctccgtaa atcccgtaag
     1081 agtttcaacg cttgtcggaa tgttgtaatt gacattcagc gtttcgggct tttcaagaga
     1141 agcctcacgg ctctctacgg ggtttattac atactttttg atagcttcaa gctgtgccgg
     1201 agcaagcttg ccgaagagca tatatacttt cgcggtacga acggtcggct tgtcgccctc
     1261 cgaaactatc tgaatacact gagcacagga gtccgccctc tggtcaaact gacccggcag
     1321 atattcaact gcaaataccg tggcgtcggt ctgaggcagg cgagcgtagg tgttgtcaag
     1381 ctgcggctct gagaaaacgg ttttttcaca gcgctcgaaa agctcttcgc tgatgttttc
     1441 cacatcgtat ctgttgaaaa tacgaagctc ttcaagcgag tctatcataa gcagtgagcg
     1501 tatatccgat aaaagcgatg cagcctcgtc tgcaaattgt tttttctttt caacgtaaat
     1561 tctgtatacc atttcgtcac ttcctgttta ttttttcagt gcggcaagcg cacgcgcacc
     1621 gatgtctttt ctgtaaaagg cgtccttaaa tttaatctgc tcggttgctt tgtaagcggt
     1681 agagagagcg gcgttaaggt cttttcccgt tgccgtaact ccgaggactc tgccgccgga
     1741 ggtcacgatt ttgccatcct tttcggcagt acccgcatgg aataccgtga cgttttccgt
     1801 gttttcgctg tcgccgaagt ctatttcata gcccttgccg taagacagcg gataaccgcc
     1861 ggacgcgata accacgcacg ccgcactttc gtccttgaac tcaacattta aaaacgagag
     1921 cgtttcatcg tgaaccgcac gcataattgt gagaaggtcg gttttgagaa gcggcaaaac
     1981 aacctgagtt tcgggatcgc cgaagcggca gttgtattct ataaccttag gaccctttgg
     2041 tgtcagcatg agaccgaagt aaaggcagcc cttaaaggtc ctgttttcat tgttcatagc
     2101 cttaactgtg ggcaggaata tcttttcggc acattccttt gcgatttcgg ccgtgtagta
     2161 ggggttcggc gcaaccgttc ccattccgcc tgtattgagt cccttgtcgc cgtcaagagc
     2221 acgcttgtgg tccatggacg aaaccatggg aactacggtt tttccgtcgg tgaaagagag
     2281 aacggaaacc tcgggacccg ttaagaattc ctcgataacg acattgttgc cgctgtcgcc
     2341 gaacttttta tcctccatta ttgtttttac ggcgtcctct gcctgctcct cggattcggc
     2401 tattataaca ccttttccga gagcgagacc gtccgcctta ataacggtcg gatattcatt
     2461 tttttctttt atgtaagaga gcgcttcttc gggatccgaa aaaact
//
LOCUS       DBMDVO010000135         2504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19725_length_2504_cov_1.358302, whole genome shotgun sequence.
ACCESSION   DBMDVO010000135 DBMDVO010000000
VERSION     DBMDVO010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19725_length_2504_cov_1.358302"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..1277
                     /locus_tag="ACZPE2_02780"
     CDS             54..1277
                     /locus_tag="ACZPE2_02780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1318804.1"
                     /translation="MLIDVLMVLIVAVVAIFGTVAIVNSSKNGWEPSVKETKEPKTKK
                     ATPVAAVSDETVTAEKKEPVKISMLSVGDNLIHDGIYEQAKKRSKNGGYDFSYAYKNI
                     ASTVEKADLATINQETIIAKSYEPSGYPLFNSPQEVGEEVKKIGFDVVNLANNHMLDK
                     GAKGLSEAIDFWNNIGGITMTGAYKDENDMNRLEYIEKDGIKIGLVGITRYTNGLSLP
                     KDSALKIIYTSDEDTIKSKIQKAKAECDIVLVNVHWGEEYTTTPNNDQRELASKMASW
                     GADVIIGHHPHVIQPVEWIDNGNGTKTLVAYSLGNFISQQNTASRVIGGMLHYDLTKD
                     YDTGKTTVDNVVFEPIVTHYVRGSHDAQIYPLSQYTDSLAKAQASRLKQNDFSIKYIN
                     DFVGKVIDKQFLNQA"
     gene            complement(1345..2007)
                     /gene="deoC"
                     /locus_tag="ACZPE2_02785"
     CDS             complement(1345..2007)
                     /gene="deoC"
                     /locus_tag="ACZPE2_02785"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005396953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1318805.1"
                     /translation="MDINEILSHCDHTNLKQTAVPNDIKRLIDEAVRYNTASVCIPPC
                     YVKLASEYAVGKMRICTVIGFPNGYNTTEVKAFEAKRALLDGADEIDMVINIGALKSG
                     NADYVENEIKTLKEVCGDKCLKVIIETCLLTEDEKKTMCRIVSAAGADYIKTSTGFSN
                     AGATHDDIKLFKKYVSPSVKIKAAGGIHTLEDAAEFLSEGADRLGTSAIVGIAEKELD
                     EN"
     gene            complement(2274..>2504)
                     /locus_tag="ACZPE2_02790"
     CDS             complement(2274..>2504)
                     /locus_tag="ACZPE2_02790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1318806.1"
                     /translation="TPFIGFVFVDEPYRGKRLSELMIQSAILYARELGYEKIYIMSGE
                     IGLYEKYGFEKLGDYETIYGSIDQLFVKSTIL"
BASE COUNT          752 a          570 c          511 g          671 t
ORIGIN      
        1 gtaataggcg gcagcaatgc cgagataata aaaataagat aggagtgttg catatgctta
       61 ttgatgtgct tatggtgctg attgtggcgg tcgtagccat attcggcaca gtggcaatag
      121 taaattcttc aaaaaacggt tgggagccgt ctgttaaaga aaccaaagag ccgaaaacca
      181 aaaaagcgac acctgttgcc gcggtttccg atgaaaccgt gactgcggag aaaaaagaac
      241 cggttaaaat cagtatgctc tcggtcggcg acaatcttat acacgacgga atttacgagc
      301 aggcaaagaa gcgttcaaaa aacggcggtt atgatttttc atacgcatac aaaaatattg
      361 cctccacagt ggaaaaagct gatttggcta ccataaatca ggaaacgata atagcaaaaa
      421 gctatgagcc atccggctat ccgcttttca actctcctca ggaggtcggc gaagaagtta
      481 agaaaatagg ctttgacgtt gtaaacctcg caaacaatca tatgcttgac aagggtgcta
      541 agggactttc ggaggctatt gatttttgga acaacatagg tggcatcaca atgaccggtg
      601 cttacaagga cgaaaacgat atgaaccgcc tcgaatacat agaaaaagac ggcattaaga
      661 taggccttgt aggcataacc cgctatacaa acggtctgtc attgcctaaa gacagcgctc
      721 tgaaaataat atacacctca gacgaggaca caatcaaatc aaaaattcag aaagccaagg
      781 ccgaatgcga tattgttttg gtaaacgttc attggggtga agagtacacg acaaccccca
      841 acaacgacca acgcgagctt gcaagcaaaa tggcgtcgtg gggtgcagat gtaattatag
      901 ggcaccatcc tcacgtcata cagcccgtag aatggataga caacgggaac ggcacaaaaa
      961 cacttgtggc atattctctc ggcaatttca tctcgcagca aaacacggca tcgagagtta
     1021 taggcggtat gcttcactac gacttgacaa aggactatga caccggaaaa acaacggttg
     1081 acaatgtggt ttttgagcct atcgtgaccc actatgtgcg gggcagccac gatgctcaga
     1141 tctatcccct ttcgcaatac accgattcgc tcgcaaaagc ccaagcctcg agactcaagc
     1201 aaaacgattt cagcattaaa tatataaacg actttgtggg taaggtcatt gataaacagt
     1261 ttttgaatca agcgtaaaag ataaaagacg gactgtaaaa catacggtcc gtctttttat
     1321 atcaggctat tacataagcg ttagttaatt ttcatctaac tccttttcgg ctattccgac
     1381 gattgcactc gtaccgagcc tgtcggctcc ctccgaaagg aactccgcgg catcctccag
     1441 cgtgtgaata ccgcctgccg cctttatttt tacggacgga gacacgtact ttttaaaaag
     1501 ctttatgtca tcatgcgttg cgccggcatt ggaaaagccc gttgaggttt ttatataatc
     1561 cgctccggcc gcacttacaa tgcggcacat tgttttcttt tcgtcctcgg tcaaaagaca
     1621 agtttcgatt attaccttga ggcatttgtc gccgcaaacc tctttcagcg tttttatctc
     1681 attttccaca tagtctgcat ttccgctttt aagcgctccg atgtttatta ccatatctat
     1741 ttcgtccgca ccgtcaagca gtgcccgctt tgcttcaaat gccttgacct cggtcgtgtt
     1801 atacccgtta gggaaaccta taacggtgca gatacgcatt ttaccgactg catattccga
     1861 agcaagcttt acatagcacg gcggaataca aacgcttgcg gtattatacc taactgcctc
     1921 atcaatcaga cgttttatgt cattcggaac ggcggtctgt tttaaatttg tgtggtcgca
     1981 atgcgataat atttcgttta tatccatttt tatcctccat cggtcagata ctgtaattat
     2041 tttaccatag actttagata ctatcaatta taaaaagtcg ccgcagaagc ttaccctcgg
     2101 ggccccctac ataaggaaat tacccaaaaa tacatgaaac agcaaagtcc gtaccctgaa
     2161 aagggtacgg actcttactt ttacggtgtc acaatccgat gcttgctaaa cctgtggaca
     2221 cagttatttt gagagtgagc tcatatgtta acctctaagc caactaccta aaactataat
     2281 attgttgact taacaaatag ctggtcaatt gagccatata ttgtttcata atcacctaat
     2341 ttttcgaagc cgtatttttc atataaaccg atttctccgc tcatgatata tattttttca
     2401 tatcccagct cacgcgcata taaaattgcg ctttgaatca tcaactctga taatctctta
     2461 ccacggtatg gttcatcaac aaaaacgaat cctataaatg gtgt
//
LOCUS       DBMDVO010000136         2502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19750_length_2502_cov_1.118701, whole genome shotgun sequence.
ACCESSION   DBMDVO010000136 DBMDVO010000000
VERSION     DBMDVO010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19750_length_2502_cov_1.118701"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="ACZPE2_02795"
     CDS             <1..391
                     /locus_tag="ACZPE2_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1318807.1"
                     /translation="MVTIFCLSSQVSSKSSGLSSGVISRITELFHITLTQHTVRKTAH
                     ALEYAALCFLFSCSWQSVFLKSQHTLSFLSAALYASSDELHQYFVSGRACQPRDVLVD
                     CLGASFGLLAFFIIYTVYTHIHKNKAR"
     gene            396..1640
                     /locus_tag="ACZPE2_02800"
     CDS             396..1640
                     /locus_tag="ACZPE2_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1318808.1"
                     /translation="MAVLKAFRALRPTPEYASKVAALPYDVMNSDEARRMVFGNPYSF
                     LHVDKAEIDLPKSVDIYDDRVYEKARENLSKLVSAEVMKQDEKPCLYIYEQTWRGRTQ
                     TGIVGCTAIDDYINDVIKKHEKTRADKEADRIRHVDTLDANTGPIFLTYRKNNEISSA
                     VEDVKEKSEPVYDFVSDDVRERVWVVSDNDTIDFIIAQFKNIPSLYIADGHHRAASAV
                     KVGLRRREQNPNYTGDEEFNFFLSVIFPSDELRILDYNRVIKDLNGLSDEEFLLELRH
                     KFDIEKHGENEYRPKRAHSFGMYMSGEWYALFAKSEIAKSLDAVKSLDVSILQDNVIS
                     PILGIDDPRTDKRIDFVGGIRGLSELSRRVDSGEGKLAFSMFPTTLDELMNIADKSMT
                     MPPKSTWFEPKLLSGLFIHYLK"
     gene            1812..2192
                     /locus_tag="ACZPE2_02805"
     CDS             1812..2192
                     /locus_tag="ACZPE2_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318809.1"
                     /translation="MLGEKMKLIRNYNGYSQRQIAEILKIERSTYASYETGRNRPDIF
                     AVQRFAKIFNVSVDYILSLDDINIYRLRDQATDYAKRHKKESVISQLSSDEKILIGLY
                     RQCDAGTKKSILEDVRKAELETDE"
BASE COUNT          707 a          486 c          583 g          726 t
ORIGIN      
        1 gatggtgacg attttttgcc tgtcttctca ggtttcgtca aaatcatcgg ggctcagctc
       61 cggggttatt tcccgtataa ccgagctctt ccatataaca ttaacacaac ataccgtccg
      121 aaaaaccgca catgcgcttg aatatgcggc actttgcttc ttgttttcgt gcagctggca
      181 gtcggtgttt ttaaaatcgc agcacacact ttcgtttttg tccgcggcac tttatgcttc
      241 gtcggacgag cttcaccaat attttgtgtc cggcagagcg tgccagccga gagacgtact
      301 tgtggattgt ctcggtgcgt cattcggact tttggcgttt tttataatat atactgtata
      361 tactcatatt cataaaaata aagcgaggta atattatggc agttttgaaa gcgttcaggg
      421 cgcttcgtcc gactccggaa tacgcttcca aggtagcggc tttgccgtat gatgttatga
      481 attcggatga ggcgaggaga atggtcttcg gcaatccgta ttcatttctg catgttgata
      541 aggcggagat agatcttccg aaatcggttg acatctatga cgaccgtgtc tatgaaaaag
      601 cacgcgagaa tttgtctaag cttgtttcgg cggaggttat gaagcaggac gaaaaaccgt
      661 gcttgtatat atatgagcag acctggcgcg gcagaacgca gaccggcatt gtaggctgta
      721 cggctattga cgattatata aacgacgtca tcaaaaagca cgaaaagaca agagccgata
      781 aggaagcgga ccgcatacgg cacgttgaca ctcttgacgc aaacaccggt cctatattcc
      841 tgacatacag aaaaaataac gagatttcgt ctgctgttga agatgtaaaa gaaaaaagcg
      901 agccggttta tgattttgtg tccgatgatg tccgtgaaag agtctgggtt gtgtctgaca
      961 atgacacgat tgattttata atcgcacagt ttaaaaacat accgtctctc tatattgcgg
     1021 acggtcatca cagagccgct tcggccgtta aggtagggct tcgccgccgt gagcaaaatc
     1081 cgaattacac gggagatgag gaatttaatt tcttcttatc cgtaattttc ccgagcgacg
     1141 agcttcgcat tcttgattac aaccgagtga ttaaggattt aaacggactt tccgatgagg
     1201 aatttttgct tgaactcaga cataaattcg acatagaaaa gcatggggaa aatgagtacc
     1261 gtcctaaaag ggcgcattcc ttcggtatgt atatgtcggg tgaatggtat gctctcttcg
     1321 ctaaatctga aatagcaaag tctttggacg ccgtcaaaag tctcgacgta tcaatactcc
     1381 aagataatgt catatcaccc atactcggta ttgatgatcc cagaacagat aagagaatag
     1441 attttgtcgg cggcattagg ggcctttccg agctttcgcg gcgcgttgac tcgggcgaag
     1501 gaaagcttgc gttctccatg ttcccgacaa ctcttgacga gcttatgaat atagccgaca
     1561 aatctatgac tatgcctccc aaatcgactt ggtttgagcc gaagctttta agcggccttt
     1621 ttatacatta tcttaaatga tgcgatgttt ttcggcggtg ccgaatgtat tcgcgaagct
     1681 tcatacacgt ttggttactt atatacatat aatttaaaaa atatttgtgt taataagtaa
     1741 cacttttcgt atattatcga atagttttat ataataaaca gtataaaagt acaggcgagg
     1801 aggtacttat tatgctggga gaaaaaatga agcttattcg taattacaac gggtattctc
     1861 agcgtcaaat agcggaaata ctgaaaattg aaaggtctac ctacgcttcg tatgaaacag
     1921 gcagaaatcg accggatatt tttgcggttc agcgctttgc aaaaatcttc aatgtcagcg
     1981 tggattatat tttaagcctt gacgatatca atatttatcg tttgcgcgat caagcgaccg
     2041 attatgcaaa gcgtcacaaa aaagaaagcg tcatttcaca gctttcatcc gatgaaaaaa
     2101 ttctgatagg cctttaccgt cagtgcgacg caggtacgaa aaaatcaatt cttgaggatg
     2161 ttcgtaaggc tgaacttgag accgacgaat aatggaatcc gttcgcggta aaatttaaga
     2221 gtttaaaggg aaccgtaaag ctgtttttac ggttccttta tctttatgcg atggtttttg
     2281 cgtttttttc acagtcttta aagaatttgt gagaaaaggg gtgcaataat cgtcttcggc
     2341 tgatataatt gtattggttt aagttggagt ttatcacagc ttttgcgata aagagacgga
     2401 aaaccgcgct gcaacgcttt gtgccggagc tgtacgttag gcaaagtgta atttaaaagc
     2461 aggagatgaa tatatgattt ccgttgccgt aataggaggc gg
//
LOCUS       DBMDVO010000137         2497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19792_length_2497_cov_1.443656, whole genome shotgun sequence.
ACCESSION   DBMDVO010000137 DBMDVO010000000
VERSION     DBMDVO010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19792_length_2497_cov_1.443656"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="ACZPE2_02810"
     CDS             complement(<1..585)
                     /locus_tag="ACZPE2_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1318810.1"
                     /translation="MSEENVKLTVSPSPHKRSVDTTRSIMLDVIIALMPALIMSVVLF
                     GFRSLLVTAVSVLSCVLFEYIARKVMKRDNTIGDLSAVVTGILLAFNLPSTMPIWMVI
                     IGDAVAIIVAKQFFGGIGQNFVNPALAGRIVLMTSFANAMSDYPAPFAWRNAAVDAVT
                     SATPLSQMTSVYKAGDIDAAINAAGLPTLTNMFFG"
     gene            complement(582..1916)
                     /gene="rsxC"
                     /locus_tag="ACZPE2_02815"
     CDS             complement(582..1916)
                     /gene="rsxC"
                     /locus_tag="ACZPE2_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1318811.1"
                     /translation="MRSEIIKPVKKVRGGVSVSHEKHTKDMQVKRIPAPETVTLPMSQ
                     HIGAPCTPTVKVGDRVLVGQVVGDTDKYVSAPVHASVSGKVTAVKEIRTAQGIVSQAV
                     IIENDGLMEEAEFTPPTVETHDDLIKAVRASGAVGLGGAGFPTHVKLNFPQDGSVDTL
                     IINAAECEPYITVDYRECLDNSWDVMSGVHLLKDMLNFKKVIIAVEDNKPDAIKILER
                     IAEKDDPDDVIKVMPLRSKYPQGAEKMMILSATGRRVPPGKLPSDVGCVVMNVTSAAF
                     ISRYLKSGKPLVSRSLTVDGSAITAPQNVRVPIGTEIDYIIKACGGFREPPVKIITGG
                     PMMGTSIVDTHHPILKCNNAILAFTDDDMSLKTETACIHCGRCAKACPMYLQPTVIHK
                     YAVQKDVKNLLKAGAMVCMECGSCAYTCPAGKPLVQFMRDAKSIVRSEAQKK"
     gene            complement(1917..2447)
                     /locus_tag="ACZPE2_02820"
     CDS             complement(1917..2447)
                     /locus_tag="ACZPE2_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019099.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1318812.1"
                     /translation="MKITKTKSYKAAFTGIAAAVAITLSFLEGLIPTAAFMPPGAKAG
                     FSNIAVMFAASEFGLIPALSVTLLKSLFVFLTRGATAFFMSLAGGVLSTLVMYLLFRF
                     SKNAGYIEIGILSALAHNAGQFSVAAIMVSGESVIGYAPVLLLSALVTGAVTGSILRA
                     VMPKLHNIDKAVKGGR"
BASE COUNT          616 a          662 c          576 g          643 t
ORIGIN      
        1 gccgaagaac atatttgtga gcgtgggaag ccccgcggca tttattgcgg catctatatc
       61 gcctgcttta tacacgcttg tcatctgtga aagaggcgtt gcggacgtga ccgcgtcaac
      121 cgcggcattt ctccaagcaa acggagccgg atagtcggac atcgcatttg caaacgacgt
      181 cataagcacg attctgcctg caagcgcagg gtttacaaag ttctgaccta tgccgccgaa
      241 gaactgcttt gcgacgataa ttgcaactgc gtcgccgatt attaccatcc agatcggcat
      301 tgtggacggc aaattgaaag ctaaaaggat accggttacc acagcactta aatcgcctat
      361 ggtattatct cttttcataa ccttgcgcgc tatgtattca aacaaaacac atgagaggac
      421 gctcaccgcc gtaacgagca gcgaacggaa tccgaacagc accaccgaca ttataagtgc
      481 cggcataagg gcgataatta cgtcgagcat tatgcttctt gttgtatcga ctgaacgctt
      541 gtgaggcgaa ggagatacgg taagctttac attttcttca ctcatttttt ctgcgcctcc
      601 gatcttacaa ttgattttgc gtcacgcata aactgaacaa gcggtttgcc ggcagggcac
      661 gtatacgcgc acgaaccgca ctccatacaa accattgcgc ccgcctttaa aagattcttt
      721 acatcctttt gcaccgcgta cttatgtatt accgtcggct gaagatacat ggggcaggcc
      781 ttagcgcatc tgccgcagtg tatacaagcc gtttccgtct taaggctcat atcgtcatcg
      841 gtgaaggcaa gaattgcgtt attgcatttt aatatcgggt ggtgcgtatc gactatcgag
      901 gtacccatca tgggtccgcc ggtaattatt ttaaccgggg gctcgcggaa gccgccgcac
      961 gccttgatga tgtaatctat ctcggtgcct atcggaacgc gcacattctg cggagcagtt
     1021 atggcagaac cgtcaaccgt aagagaacgc gatacaagcg gcttgcccga ttttaaatag
     1081 cgtgaaataa atgccgccga tgtcacgttc ataacaacac agcccacgtc cgagggaagc
     1141 tttccggggg gaacgcgtct gcccgttgcg gagagtatca tcattttctc tgcaccctgc
     1201 gggtattttg agcgaagagg cataaccttg attacgtcgt cggggtcgtc cttttcggct
     1261 attctctcaa gaattttgat ggcatcgggc ttgttgtcct caaccgctat tatgaccttc
     1321 ttgaaattga gcatatcctt taaaagatgt actccgctca ttacgtccca cgaattatcg
     1381 agacattcgc ggtagtcaac cgttatatac ggttcgcatt ccgccgcgtt tattataagt
     1441 gtgtcaacgc tgccgtcctg cgggaaattg agctttacat gagtcggaaa gcccgctccg
     1501 ccgagtccta ccgcgccgga agctctgact gcctttatca ggtcatcgtg agtttcgacc
     1561 gttggcggtg taaactccgc ctcctccata agcccgtcgt tttcgataat gacggcttga
     1621 gacacaatac cttgagcggt tctgatttcc tttaccgccg taaccttacc cgacacggac
     1681 gcgtgcacgg gtgcgcttac gtacttatcg gtatcgccta ctacctgacc tacaagaact
     1741 ctgtcgccga cctttaccgt tggcgtacac ggcgcgccga tatgctggct catcggaagc
     1801 gtgaccgttt ccggagccgg aatgcgttta acctgcatat ccttggtgtg cttttcgtgc
     1861 gagacactta caccgcctct gacttttttt acaggtttta ttatttcaga gcgcattcat
     1921 cttcctccct ttacagcttt atctatatta tgaagctttg gcataacggc cctcaatatg
     1981 cttcccgtga cggcccccgt aacaagagcc gacaaaagaa gcaccggggc atagcctata
     2041 accgactctc cggaaaccat aatcgcggca accgaaaact gccctgcatt atgagccaaa
     2101 gccgacaaaa tacctatttc tatatatcct gcgtttttcg aaaatctaaa taacaaatac
     2161 atcacaaggg tgctgagcac tccgcctgcg agtgacataa aaaatgccgt ggcaccgcgc
     2221 gtaagaaaaa caaacagcga ctttaaaagc gtcaccgaca gtgccggtat aagcccgaat
     2281 tccgaggcgg caaacataac cgctatattg gaaaaacctg ccttggctcc gggcggcata
     2341 aacgcagctg tcggtatcag cccctcaaga aacgagagtg ttattgccac agccgcagct
     2401 atacccgtaa atgcggcctt atatgatttt gtcttagtta tcttcatatc aatatgtaat
     2461 cacgtctata ttttcggtag tgccctttat gcttacg
//
LOCUS       DBMDVO010000138         2494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19825_length_2494_cov_1.255746, whole genome shotgun sequence.
ACCESSION   DBMDVO010000138 DBMDVO010000000
VERSION     DBMDVO010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19825_length_2494_cov_1.255746"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..745)
                     /locus_tag="ACZPE2_02825"
     CDS             complement(8..745)
                     /locus_tag="ACZPE2_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR1318813.1"
                     /translation="MIITAGKRSFQELNNEIRGMLLSEDMIRVEDVNGQRYIGAALDK
                     GKTIEIHGTPGNDMGAYLNGGNIEVYGNGQEAVGNTMGGGSVTIHGHVGDTLGYAMRD
                     GDIFVEKRAGSRAGIHMKEFHELLPVVVIGGVAGAFLGEYMAGGILIVLGLDNSEKLP
                     IVGPHCATGMHGGRIFIRGEVPQENISEHITAVKELDSRDTDELQRHVRAYCEKFGKS
                     FDEIMSVPFTKLVPTTSRPYANLYTPN"
     assembly_gap    909..918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<919..2264)
                     /locus_tag="ACZPE2_02830"
     CDS             complement(<919..2264)
                     /locus_tag="ACZPE2_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR1318814.1"
                     /translation="MDFTVPEFDIERNRDRCTLCGACVKECSYKVHFFSKDKKAVLAD
                     ERKCVACHRCAVICPSHAIKIVKYDMAYRDHANWTPTAQKEIIKQATTGGVLLSGMSN
                     NKPYPIYWDKMLLNASQVTNPSIDPLREPMEIRTFLGRKPDKIEFTEDGRLKTVMPPQ
                     VKLETPIMFSAMSFGSISFNAQKTLAMAAKELGTIFNTGEGGLHPGLTDFTDYAAVQV
                     ASGRFGVHKQYLNNCRFIEIKIGQGAKPGIGGHLPGEKVTVEVSNARMIPSGSDAISP
                     APHHDIYSIEDLRQLIWSLKEATQYKKPVAVKIAAVHNCSAIVSGVARAGADIIVMDG
                     FRGGTGAAPTRIRDNVGIPIELALAAVDQRLRDESIRNSVSLVVSGSFRNSADVVKAI
                     ALGADAVYVATAPLIAMGCHMCQQCHTGKCNWGIATQRPELTKRLDPDEMAPRVVNL"
     gene            complement(2264..>2494)
                     /locus_tag="ACZPE2_02835"
     CDS             complement(2264..>2494)
                     /locus_tag="ACZPE2_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318815.1"
                     /translation="ALINGPFSILVGSKHGLLALNDRLKLRSLITAEKGNKTYFASEE
                     SAIRIICPDPEKIAAVDGGTPIFIPFAREGDR"
BASE COUNT          560 a          675 c          575 g          674 t
ORIGIN      
        1 ggttttatca gttaggtgtg tagagatttg catacggacg cgaagtagtg ggaacaagct
       61 ttgtgaacgg cacggacata atttcatcaa aactcttgcc gaacttttca caataagcgc
      121 gcacatgcct ttgaagctca tccgtatcgc ggctgtcaag ctcttttacg gcggttatat
      181 gctccgatat attctcctgc ggcacctcac cgcgtatgaa tattctaccg ccgtgcatac
      241 cggtagcaca atgcggtccg acaatcggca gcttttccga attgtcgagt ccgaggacta
      301 tgagtattcc gccggccata tattcgccga ggaatgcacc tgcgacgccg cctatgacca
      361 ccaccggcaa aagctcgtga aactctttca tatgaattcc cgctcttgag cccgcgcgtt
      421 tttcgacaaa aatatcgccg tcgcgcatgg cgtagccgag agtatcgccg acatgaccgt
      481 gtattgtaac tgagccgccg cccatggtat tgccgacagc ctcctgaccg tttccgtaca
      541 cctcaatatt gccgccgttt aaatacgcgc ccatatcgtt gccgggcgtt ccgtgtattt
      601 caatggtttt gcccttatcg agtgctgcgc ctatatacct ttggccgttt acgtcctcga
      661 cgcgtatcat atcttcactt aaaagcattc cgcgtatctc attatttaat tcctgaaagc
      721 tgcgttttcc ggctgttatt atcatttcgg acacctccgg ttaatttctg atttgcttat
      781 tcgcccgcat ggcgaattcc gagtatttca agttctttgt cattaaggcc gatacctcgc
      841 agcataagtc tgttgcccct taaagaatct atcgagttaa gccccatgcc gccgagcatt
      901 tcctttatnn nnnnnnnnag atttaccacg cgcggagcca tttcgtccgg atcaagtctt
      961 ttcgtaagct cgggccgctg agttgcgata ccccaattac acttgccggt gtggcattgc
     1021 tggcacatat ggcagcccat ggcaatcaaa ggtgcggttg cgacatatac cgcgtccgct
     1081 ccgagggcta tggccttgac aacatcagca gaatttctga aagagcccga tacgacaagc
     1141 gaaaccgaat tccttatact ctcatccctg agcctttggt ctaccgccgc caaggcgagt
     1201 tctatcggta taccgacatt atcccttatt ctcgtgggag ccgctccggt tccgccgcgg
     1261 aagccgtcca taactattat atcggcacct gcgcgcgcaa cgcctgaaac tatcgccgag
     1321 cagttgtgta cggcggctat tttcaccgca acgggttttt tatactgggt ggcctccttt
     1381 aaagaccaaa taagctggcg caaatcctct atcgaatata tatcgtggtg cggagcggga
     1441 gatatggcat ccgaaccgga cggtatcata cgcgcattgc ttacctccac ggtaaccttt
     1501 tcgcccggaa gatgtccgcc tatgccgggc tttgcgccct gccctatctt gatttcgata
     1561 aaccgacagt tatttaaata ctgcttgtga acgccgaatc tgcctgacgc aacctgtact
     1621 gcggcataat cggtaaaatc tgtaagaccc gggtgaagtc cgccctcacc ggtattaaaa
     1681 atcgttccga gctctttggc tgccatagcg agcgtttttt gagcattgaa cgaaatcgag
     1741 ccgaaggaca ttgccgaaaa cataatcggg gtttcgagct ttacctgcgg cggcataacc
     1801 gtcttgagcc ttccgtcctc ggtgaactct atcttgtcgg gctttctgcc gaggaaggtt
     1861 cttatctcca taggctctct caaggggtct atggacgggt ttgttacctg actcgcattt
     1921 aaaagcattt tatcccaata aatcggatac ggcttattgt ttgacatacc cgagagcaaa
     1981 acgccgccgg tagtcgcctg ctttataatt tctttctgcg ccgtcggagt ccagttagcg
     2041 tggtcgcgat acgccatatc gtattttacg atttttatcg cgtgcgacgg gcaaatcacg
     2101 gcacagcgat ggcacgccac acactttctc tcatcagcca aaacggcttt tttatccttt
     2161 gagaaaaaat gcaccttgta cgagcattcc ttgacgcatg caccgcaaag cgtacagcgg
     2221 tcgcggtttc tctctatatc aaattcggga acggtaaaat ccattatctg tcgccctccc
     2281 ttgcaaacgg aataaatatc ggagtgccgc cgtcaaccgc ggctattttt tcgggatcgg
     2341 ggcaaattat gcgtatagcc gattcctctg atgcgaaata tgttttgtta cccttttctg
     2401 cagtgataag cgaacgcagt ttaagcctgt cgttaagagc gagcaagccg tgctttgagc
     2461 ctaccaatat cgagaacgga ccgtttatca gcgc
//
LOCUS       DBMDVO010000139         2483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_19953_length_2483_cov_1.177162, whole genome shotgun sequence.
ACCESSION   DBMDVO010000139 DBMDVO010000000
VERSION     DBMDVO010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19953_length_2483_cov_1.177162"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="ACZPE2_02840"
     CDS             <1..262
                     /locus_tag="ACZPE2_02840"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR1318816.1"
                     /translation="YFAHLRNIKVSDRHFNETAHESSEGSLDMYEIVKALQDVGFDGY
                     VRPDHGRMIWGEVARPGYGLYDRALGAAYLNGLWEAVEKSKK"
     gene            294..1142
                     /locus_tag="ACZPE2_02845"
     CDS             294..1142
                     /locus_tag="ACZPE2_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1318817.1"
                     /translation="MKHENLNGRVAVVTGGGGVLCGDFSKVLAKQGVKVAVLDLNEAA
                     AQKVVDEITADGGTAIAVGCNVLDPESMRKAREVVNEKLGTCDILLNGAGGNNPKGTT
                     TKETLEKIDLTQHDENIKTFFDLDPQGISFVFNLNFLGTLIPTQVFARDMAQKENTCI
                     VMISSMNAYRPLTKIPAYSAAKAAVSNFTQFMAVHFADVGIRVNAIAPGFFSTNQNKA
                     LLWNEDGTPTARTGKILAATPMKRFGEPNELDGTLLFLCDEDYSGFITGTTIPVDGGF
                     NAYSGV"
     gene            1165..2262
                     /locus_tag="ACZPE2_02850"
     CDS             1165..2262
                     /locus_tag="ACZPE2_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1318818.1"
                     /translation="MNLNLKDAASCKYDMISLGEIMLRLDPGEGRIKTARSFRAWEGG
                     GEYNVARGLRRCFGMRTAAVTALADNEVGRLVEDFMLEGGVDTSLIKWVPYDGIGRTV
                     RNGLNFTERGFGIRGALGVSDRGNTAVSKLKPGDIDWEHIFGELGVRWFHTGGIFAAL
                     SETTAEVVIEAVKTAKKYGTIVSYDLNYRPSLWKDIGGKEKAQEVNKKIAKYVDVMIG
                     NEEDFTACLGFEVEGNDANLKKLNIDGYCKMLSEVVKVYPNFKAIGTTLRTVKTATIN
                     DWSAMLYADGSVHKGMSLPSLEIMDRVGGGDSFASGLIYGLMTTGDPDKAVNYGIAHG
                     ALAMTTPGDTSMASLKEVENIVSGAGARVQR"
BASE COUNT          677 a          576 c          635 g          595 t
ORIGIN      
        1 ttattttgca caccttcgca acatcaaggt ttcggacaga catttcaatg agacggctca
       61 cgagtcgagt gagggttcgc ttgatatgta cgagattgta aaggctcttc aggatgtcgg
      121 atttgacgga tatgtgcgtc ccgaccacgg cagaatgatt tggggcgaag tcgcaagacc
      181 cggctacggt ctttacgaca gagcactcgg cgccgcatac cttaacggat tatgggaagc
      241 cgtagagaaa tcaaaaaagt aattaaaggt acgaaccgaa aggactggac tttatgaaac
      301 acgaaaattt aaatggcaga gttgcggtag taacgggcgg cggcggagtt ctctgcggag
      361 acttttcaaa ggtgctcgca aagcagggtg taaaggtagc cgtgcttgat ttaaacgaag
      421 cggcggctca aaaggttgta gatgagataa ccgcagacgg cggaacggca attgccgtag
      481 gctgcaacgt gcttgacccc gagagtatgc ggaaggcgcg cgaggttgta aacgaaaagc
      541 tcggcacctg cgacattctc ctcaacggag cgggcggtaa caacccaaag ggtaccacga
      601 ccaaagaaac tctcgaaaag atagatttga ctcagcacga cgagaacatc aagacatttt
      661 ttgatcttga cccacaggga atttcgtttg tgtttaacct caatttcctc ggaacgctta
      721 ttccgactca ggtatttgcg cgcgatatgg ctcagaaaga aaacacctgc atagttatga
      781 tttcttctat gaatgcctat cgtccgctaa cgaagatacc ggcatattcg gctgcaaagg
      841 cggctgtttc aaacttcacg cagtttatgg ctgttcattt tgccgatgta ggtatcagag
      901 ttaatgccat tgcgccgggc ttcttctcga ccaatcaaaa taaagcactt ctttggaacg
      961 aggacggcac gcccacggca cgcaccggca aaattctcgc tgcaactccg atgaaacgtt
     1021 tcggcgagcc gaacgagctt gacggcacgc ttctgttcct ctgtgatgag gattattcgg
     1081 gctttataac cggtactaca attccggttg acggtggatt taacgcttat tccggagttt
     1141 aagctccgtt tatgggagaa tattatgaat cttaatttaa aagatgcagc ttcctgcaaa
     1201 tatgatatga tttcgctcgg cgaaataatg ctgagacttg atcccggcga aggcagaatc
     1261 aaaaccgcgc gttctttcag agcatgggag ggcggcggcg aatacaatgt cgcaagagga
     1321 ctcagacgtt gcttcggtat gcgtaccgcg gctgttacgg ctcttgccga caatgaggtc
     1381 ggcaggctcg tagaggactt catgttagag ggcggagttg acacttcact tataaaatgg
     1441 gtcccgtatg acggtatagg aagaactgtt cgcaacggtc ttaactttac cgagcgtggt
     1501 ttcggcataa gaggtgctct cggagtttct gaccgcggaa ataccgctgt ttcgaaatta
     1561 aagcccggtg atattgactg ggagcacatt ttcggtgagc tcggcgttcg ttggtttcac
     1621 accggcggaa tttttgccgc actttccgaa actaccgcag aggttgttat agaggctgtt
     1681 aaaacagcca aaaaatacgg aacaatcgtt tcctacgacc tcaattaccg tccgtccctc
     1741 tggaaagaca tcggcggcaa ggaaaaggcg caggaagtaa ataagaagat agcaaaatat
     1801 gtagacgtta tgataggcaa tgaggaggat ttcaccgctt gtctcggctt tgaagtagag
     1861 ggcaatgacg caaatcttaa aaagctcaat atcgacggct actgcaaaat gctttccgaa
     1921 gtcgttaagg tgtaccccaa tttcaaggct atcggtacca ccttgcgcac agtgaaaacc
     1981 gcaacaataa acgactggtc cgcaatgctc tatgccgacg gcagcgttca taaggggatg
     2041 tctcttccgt cgcttgaaat tatggaccgc gtcggcggcg gagacagctt tgcctccggt
     2101 cttatctacg gccttatgac caccggcgac cccgacaaag ccgtaaatta cggcatagct
     2161 cacggtgctc tcgcaatgac cacacccggt gacacatcta tggcgtcgct caaggaagta
     2221 gagaatatcg tatccggcgc gggtgcacgc gttcagaggt aaaaccttac gcatcttacg
     2281 acttttcggc gcccaatctc gcatatcaaa aatatgcttc ggggcatcaa aaagccgtaa
     2341 tatacgaaat cttttccctc tgaaatcaag tggtaaaatc ttacgcatat cagggctttt
     2401 cggcgaccga acttgcatat caaaaatatg ctttggggca tcaaaaagcc gtaatatacg
     2461 aaatcttttc cctctgaaat caa
//
LOCUS       DBMDVO010000140         2467 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20135_length_2467_cov_1.090871, whole genome shotgun sequence.
ACCESSION   DBMDVO010000140 DBMDVO010000000
VERSION     DBMDVO010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20135_length_2467_cov_1.090871"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="ACZPE2_02855"
     CDS             complement(<1..578)
                     /locus_tag="ACZPE2_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TroA family protein"
                     /protein_id="HIR1318819.1"
                     /translation="MKKAKRVLALLLCLVLILSAVGCSAKKDDGKKSSDSEVVTVVDN
                     NGNTVTVKKDIQRIVVCDIYPIPSVLAVFFDSASKIVGMAQPSMAAAKNSLLSELYPE
                     ILNAKTGFIDGTTVNMEALAALNPDVVFYSSGNKELGKQLTDAGYAAIAISANKWQYN
                     AIETLNNWIDLLSQLFPENNKSEKVRTYSESV"
     gene            complement(638..1432)
                     /locus_tag="ACZPE2_02860"
     CDS             complement(638..1432)
                     /locus_tag="ACZPE2_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318820.1"
                     /translation="MTLTVENGCFSYDGRNTVLSDISFCASPGEIVAILGKNGSGKTT
                     LLKCSVGLLKWSGGHSFLDGRDVLHIKSRELWSKISYVPQAKSSFGGYTVLDSVLLGF
                     GSSIGIFGKPRKSDVEKALSVLRRLNIENLAYKKCSDLSGGEKQMVLIARAIACDPEI
                     LILDEPESNLDFKNQITVLDVLSKLRDSGICCIFNTHFPDHAMRIADRALLLGDDGRC
                     ICGKTNDIVTEDNIQKTFGVNVKIAEVTSGGRIVKTIVPVSAADGS"
     gene            complement(1429..>2467)
                     /locus_tag="ACZPE2_02865"
     CDS             complement(1429..>2467)
                     /locus_tag="ACZPE2_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1318821.1"
                     /translation="NRDMTGRKECKGLELSTLSKALLLILPLVVAVLALCIGRLGISP
                     AEVIRSIFGKITGNADLESKNELVLWNIRMPRIILAALVGAGLSVSGCTFQSLFSNPL
                     ATPDTVGVSSGTCFGAAVAIFFGADFIVTQAVAFLFGIIAVLLTYLAGSGKGKSLNSV
                     VLAGIMIGSLFSALVSFIKSVADVNSVLPVITFWLMGSFAGAGYRSLMLGAPVMLVGM
                     AVLYVIRWRLNILTLPDDEAKATGTDIKALRAVSVICATAITASCVSMCGQVGWVGLL
                     VPHMCRMKFGNNHVSLLPACISMGMTFMIIVDTVARSATAAEIPISVLTAVIGAPFFI
                     ILMRKTGGWQL"
BASE COUNT          666 a          645 c          502 g          654 t
ORIGIN      
        1 tagacactct cgctgtaagt tcttactttt tccgacttat tgttttcagg gaaaagctga
       61 gagagaaggt ctatccagtt attgagggtt tcaatggcat tatattgcca tttattcgct
      121 gaaatcgcta ttgcggcata gcctgcatcg gtaagctgct tgccgagctc tttattgccc
      181 gagctgtaaa ataccacgtc gggatttaaa gccgcgagtg cctccatatt aacggttgtt
      241 ccgtctataa atccggtctt ggcattgagt atttccggat aaagctccga caaaaggctg
      301 ttttttgcag ccgccatact cggctgtgcc ataccgacta tttttgaagc cgaatcaaag
      361 aacacggcaa gcaccgacgg tatagggtaa atatcgcata ccactattcg ctgaatgtcc
      421 tttttaacgg ttacggtatt accgttattg tcaacaacgg tgacaacctc gctgtcactg
      481 ctctttttgc cgtcgtcttt tttagcggag cagcctaccg ccgacaaaat aagcacaaga
      541 caaagaagca aagctaatac tctttttgct tttttcattt acattacctc tttcttaatt
      601 atattaaaaa tgctcttcgc atttatttcg gattttttta ggaaccgtcg gccgcactta
      661 cgggcactat cgttttaacg attctgccgc ccgacgttac ctcggctatt ttaacattca
      721 caccgaatgt cttttgtata ttatcctctg tcactatatc gtttgtcttg ccgcaaatac
      781 atcttccgtc atctccgaga agcaatgccc tatccgctat tctcatcgcg tgatcgggga
      841 aatgagtatt gaatatacaa caaatcccgc tgtcgcgaag ctttgacaaa acatcaagaa
      901 ccgttatctg atttttaaaa tcgaggttcg actccggctc gtccaaaatc aaaatttcgg
      961 ggtcgcacgc tattgctctc gctatcaaga ccatctgctt ttcgcctccg gataaatccg
     1021 agcatttttt ataggcgaga ttttctatat tcagccttct taaaacggaa agcgcttttt
     1081 ccacatcgct ttttcgcggc tttccgaaaa tcccgatgct gctcccgaag ccgagcaaaa
     1141 cgctgtcaag cacggtatag ccgccgaatg aggattttgc ctgcggcacg tatgatattt
     1201 tgctccaaag ctccctgctt tttatatgca aaacatctct gccgtcaagg aagctatgtc
     1261 caccgctcca tttgagcaag cccacgctgc acttgagaag cgttgttttg cccgagccgt
     1321 tttttccgag aattgccact atctctcccg gtgacgcgca aaagcttatg tcggacagca
     1381 ccgtgttcct gccgtcatat gaaaaacaac cgttttcaac agtaagggtc acaactgcca
     1441 cccccctgtt tttcgcataa gaattataaa gaacggtgcg cctataactg cggtcagcac
     1501 agagatggga atctcggcgg ctgtggcact tcgcgcaacg gtatctacaa ttatcataaa
     1561 ggtcataccc atgctgatac aggccggcag cagcgacaca tgattgtttc cgaatttcat
     1621 acggcacata tgcggcacca gaagccctac ccagcccacc tgaccgcaca ttgaaacgca
     1681 cgaggcggta attgccgttg cacatattac cgaaacggca cgcagagcct taatatccgt
     1741 gccggtcgcc tttgcctcat catcgggaag agttaaaata ttaagccgcc atcttataac
     1801 atacaagaca gccattccga caagcattac cggcgcgccg agcataagcg agcgatagcc
     1861 tgcgcccgca aagctgccca taagccaaaa ggttattacc ggaagcacgg aatttacgtc
     1921 cgccaccgat tttataaacg agacaagtgc cgaaaaaagc gagcctatca taattcctgc
     1981 caaaaccacg ctgttaagtg atttgccttt accgctacct gcgagataag tcagcaacac
     2041 ggcaataatt ccaaacaaaa aagcaactgc ctgagtgaca ataaaatcag ccccgaaaaa
     2101 aatcgcaaca gccgctccga agcaggttcc gcttgaaact ccgactgtat cgggcgtggc
     2161 aagaggattt gaaaaaaggc tttgaaatgt acagcccgaa accgaaagtc ccgctcccac
     2221 aagggctgca aggattattc gcggcattct tatattccat aaaacaagct cgtttttaga
     2281 ttccaaatca gcatttcccg ttattttacc gaatatcgat cttatcacct cggcgggaga
     2341 aataccgagt ctgcctatac aaagcgccaa aacagcgaca acaagcggca gtattaaaag
     2401 cagggccttc gaaagcgtgg aaagctccaa gcctttacat tcttttcttc ctgtcatatc
     2461 tctgtta
//
LOCUS       DBMDVO010000141         2462 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20193_length_2462_cov_1.224481, whole genome shotgun sequence.
ACCESSION   DBMDVO010000141 DBMDVO010000000
VERSION     DBMDVO010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20193_length_2462_cov_1.224481"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="coaE"
                     /locus_tag="ACZPE2_02870"
     CDS             complement(<1..490)
                     /gene="coaE"
                     /locus_tag="ACZPE2_02870"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1318822.1"
                     /translation="MLVIGVTGQTGAGKGTVCKMLEKYGLYHIDADKVAHSVYKKGSD
                     VLSALSEAFGEDILNTDGTANRAKIAEKAFSSAENTEKLNSIVHPAVTQKIKAIIKEQ
                     SSLGTKGVLLDAIALFESGENRLCDFTFAVIAPEKMRLERIMVRDGIEKNAALRRIRA
                     QKD"
     gene            complement(505..1527)
                     /locus_tag="ACZPE2_02875"
     CDS             complement(505..1527)
                     /locus_tag="ACZPE2_02875"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1318823.1"
                     /translation="MQTKLIKANDSNINETALLAGEILRNGGIVAIPTETVYGLAANA
                     LSGEAVKKIFEAKGRPQDNPLIVHVTDISEVTPLVKSIPDEARRLAERFWPGPLTMIM
                     PKSDLIPDETSGGLSTVAVRCPSHPVARAIIRAAGVPLAAPSANVSGRPSPTNAKYVF
                     EDMNGKIDMIVDGGQSEVGVESTVVTLAQKPARLLRPGGITAEQLKSVLGEVVIDDAV
                     LHMLKSGAKAASPGMKYKHYAPKADVTIVKGSFDAFRRFVAEKGDCFVLCFEGEEKYF
                     KNYVTYGRADDPSSQANRLFDALRELDEKGAKTVYARDPGTSGVDLAVYNRLIRAAAF
                     KTADAK"
     gene            complement(1664..1990)
                     /gene="hisE"
                     /locus_tag="ACZPE2_02880"
     CDS             complement(1664..1990)
                     /gene="hisE"
                     /locus_tag="ACZPE2_02880"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR1318824.1"
                     /translation="MEDVLTELYEVVTQRHENPQEGSYTCYLFDQGLDKILKKVGEES
                     AETIIAAKNGIKEDTVGEISDLLFHLVVMMKNEGITPDDVKAELSKRHQKSGNLKKFH
                     QVDKNT"
     gene            complement(1991..2317)
                     /gene="hisI"
                     /locus_tag="ACZPE2_02885"
     CDS             complement(1991..2317)
                     /gene="hisI"
                     /locus_tag="ACZPE2_02885"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR1318825.1"
                     /translation="MDLDKYFVKSELIPAVIQEHGTKKVLMLAYMNKESLAKTLETGY
                     TWFFSRSRQKLWNKGETSGHTQKVVSIKGDCDDDTLLIEVIQKGPACHTGSKTCFFKE
                     IKSEVK"
     gene            complement(2320..2460)
                     /locus_tag="ACZPE2_02890"
     CDS             complement(2320..2460)
                     /locus_tag="ACZPE2_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013168.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR1318826.1"
                     /translation="MTASGGIKDSGDIKALKDAGLYGAICGRSIYKGTLDLREALEIC
                     RE"
BASE COUNT          548 a          659 c          537 g          718 t
ORIGIN      
        1 catccttttg tgcccttatg cgccgaagtg cggcattttt ttcgatgccg tcgcgtacca
       61 taatccgctc aaggcgcatt ttctccggtg cgataaccgc aaatgtaaaa tcacaaagcc
      121 gattttcgcc gctttcaaaa agcgctattg cgtccaaaag gacccccttt gtgccgaggg
      181 agctttgttc ttttattatt gcctttattt tttgagtgac agccgggtgg acgatgctgt
      241 tcagcttttc cgtattctcg gcggacgaaa acgccttttc ggctatttta gccctgttcg
      301 ccgtgccgtc cgtatttaaa atatcttcgc cgaaggcttc ggaaagagcc gaaagcacat
      361 cggagccctt tttgtaaacc gaatgtgcca ccttgtctgc gtctatgtgg tacagcccgt
      421 atttttcaag cattttgcaa accgtaccct tgccggcacc cgtttgcccg gtaactccta
      481 tcacaagcat tcaagacacc tcacttattt tgcgtccgcc gtcttaaatg ccgcagcgcg
      541 gataagacgg ttgtatacgg caaggtctac gccgctcgtc ccggggtcac gtgcataaac
      601 ggtttttgcg cccttttcgt caagctcacg cagagcgtca aacaatctgt tagcctgtga
      661 cgaggggtcg tcggctctgc cgtatgtgac ataattttta aaatattttt cctcaccctc
      721 aaagcatagc acaaagcagt cacccttttc cgctacaaaa cggcgaaaag cgtcgaaact
      781 gcctttgaca atcgtaacgt ctgccttggg tgcgtaatgc ttatacttca tacccggaga
      841 cgcagctttt gctcccgact tcagcatatg gagcacagcg tcgtctataa ccacttcgcc
      901 gagcacgctt ttaagctgtt ctgcggtaat tccgcccggt cttaaaagtc ttgccggctt
      961 ttgagccaat gttaccacgg ttgattcaac accgacctcg ctctgaccgc cgtcaactat
     1021 catatctatt ttgccgttca tatcttcaaa aacgtatttt gcgtttgtgg gactcggtct
     1081 gcccgaaaca tttgcgctcg gcgcggcaag cggaactccc gccgccctta ttattgccct
     1141 tgccacgggg tgtgacgggc aacgcacagc cacggtcgaa agccctcccg atgtctcgtc
     1201 gggaatgagg tcgctcttcg gcattatcat ggtgagaggt cccggccaga acctttcggc
     1261 taggcggcgc gcttcgtcgg gtatgctctt aacgagcggc gtaacctctg atatatccgt
     1321 aacgtgaact atcagcggat tatcctgcgg cctgcccttt gcctcaaaaa tcttttttac
     1381 tgcctcgccg gacagcgcgt ttgcggcaag tccgtacacg gtttcggtgg gtattgccac
     1441 gataccgccg tttctgagta tctcgcctgc gagaagtgcc gtctcattta tattgctgtc
     1501 atttgcttta ataagtttag tctgcattat tatctgccct atattaaaaa tcaaaatgct
     1561 ttaataaaat attttatcat tatacgacaa taaaaacaag taaataacaa aaaagcgtcc
     1621 gcaaaacggt aaaattccgt ttgcggacgc caaacagcga ctttcaggta tttttatcca
     1681 cctgatgaaa ttttttgagg ttgccggatt tttgatgacg ctttgacagc tccgccttta
     1741 cgtcgtcggg tgtgattccc tcgtttttca tcataacaac aaggtggaag agcaggtcgg
     1801 aaatctcgcc gacggtatcc tcctttatgc cgtttttggc ggcaattata gtctcggcgg
     1861 actcttcgcc gacctttttc agtattttat cgagcccctg gtcaaaaagg tagcaggtat
     1921 aagagccctc ctgcggattt tcgtgccttt gtgtaactac ctcgtaaagc tccgtaagta
     1981 cgtcttccat ttactttacc tccgatttta tttccttaaa aaaacaggtc ttgctgcccg
     2041 tatggcaggc agggcctttt tgtattacct ctataagcag cgtatcgtcg tcgcagtcgc
     2101 cctttatcga gacaaccttt tgcgtatgac ccgaggtttc gcccttgttc cacagctttt
     2161 gccttgaacg cgagaaaaac cacgtatagc ccgtctcgag tgttttggca agcgactcct
     2221 tattcatata cgcgagcata agcacctttt ttgtgccgtg ctcctgaatc acagccggaa
     2281 tgagctccga cttaacaaag tatttatcta aatccatatt tactccctgc aaatttcaag
     2341 agcttctctt aaatcaagtg tgcccttata tatagatctt ccgcaaatcg caccgtacaa
     2401 cccggcgtct ttgagagctt ttatatcgcc ggaatccttt attccgccgc tcgcggtgat
     2461 gt
//
LOCUS       DBMDVO010000142         2461 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20219_length_2461_cov_1.034322, whole genome shotgun sequence.
ACCESSION   DBMDVO010000142 DBMDVO010000000
VERSION     DBMDVO010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20219_length_2461_cov_1.034322"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(121..867)
                     /locus_tag="ACZPE2_02895"
     CDS             complement(121..867)
                     /locus_tag="ACZPE2_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689900.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HIR1318827.1"
                     /translation="MIIYFTGTGNSRHLAKKVGEAINEIPVNSFEYIKECKKGSFDSD
                     TPYVFVCPTYAWQIPHIFEKLIRESVFHGSDKAYFIMDCGGEIGNARKQLQALCSDKG
                     FEFMGVYEVVMPENYTALYSAPDEKTAEKLIIKADKAALDATEYIKAEKPFPDIKVGI
                     ADRLKSGIVNTVFYKFIVSADKFYATDKCIACGKCVRSCVLNNIELKNGKPVWGKNCT
                     HCMACINFCPTNAIEYGKHSIGLRRYTLTD"
     gene            complement(904..1491)
                     /locus_tag="ACZPE2_02900"
     CDS             complement(904..1491)
                     /locus_tag="ACZPE2_02900"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HIR1318828.1"
                     /translation="MILQYIDSLRRGNADTLLYYYTVQSGILCFLYFFFLAVFSPKKE
                     KAVIRGAVTMCIAVTAIAYLFMPNGAKEAAASDKAFYTGYILMHYIVPLMVILDYLLF
                     FPKGLYKALHPLCWLILPYLYIAFTMICAKFGSKIFSGFGGSSRYPYPFLDTDLYGKN
                     KVALIIVFITAAFLALGYPSFVIDRLLGKNGKKLK"
     gene            complement(1799..>2461)
                     /locus_tag="ACZPE2_02905"
     CDS             complement(1799..>2461)
                     /locus_tag="ACZPE2_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1318829.1"
                     /translation="DRILTAHPEIEIVQLQINYLDWDNPGIQSRLCYETAVKHGKKVI
                     VMEPVKGGTLAKIPDEAEKLLKDYAPDASIPSWAVRFAASHDSVMTVLSGMSTYEQLL
                     DNTGYMENFKPLSKEEFEIIGKAVDIIKASITVPCTGCSYCTAGCPKHIPIPKYFALY
                     NEEKRATYKDFSVNGQYYENLAKEYGKASDCIKCRKCEKACPQHIKVSELMPQVAKTF
                     EE"
BASE COUNT          626 a          538 c          521 g          776 t
ORIGIN      
        1 aaaaacagac cttgtgattt gtccctacaa ggtctgcttt atctatgtct ttaatttata
       61 aaaataccgc ttggttatga aacgctttat gagcactcgg taaaagcaat tttccgtatt
      121 tcagtctgtc agagtgtaac gcctcagccc gattgaatgc ttgccgtatt caatggcatt
      181 tgtgggacaa aagttaatac acgccataca gtgtgtgcag tttttgcccc aaacaggctt
      241 gccgttctta agctcaatgt tgttcagcac acagcttctt acgcattttc cgcaagctat
      301 gcatttgtcg gtggcgtaga atttgtctgc ggatacgata aatttgtaaa agaccgtatt
      361 tactatgccg ctttttagtc tgtccgcaat gccgacttta atatcgggga aaggcttttc
      421 ggctttgatg tattctgttg cgtcaagagc cgctttatcc gcttttatta taagcttctc
      481 tgcggttttt tcatcaggtg cgctgtaaag tgccgtataa ttttcgggca ttacaacctc
      541 gtaaacaccc ataaattcga aacccttgtc agagcaaagc gcttgtaatt gtttccttgc
      601 atttcctatt tcgccgccgc aatccattat gaaatatgct ttatcggagc cgtgaaacac
      661 ggattccctt atgagctttt caaaaatgtg cggtatttgc caagcgtaag tcgggcatac
      721 aaaaacatag ggggtatcgc tgtcaaaact gcctttttta cactccttta tatattcaaa
      781 tgagttgacc ggaatttcgt ttattgcttc tccgaccttt tttgctaaat ggcggctgtt
      841 tccggtgcct gtaaaatata ttatcacttt tatcgctcct tcaaatatgt tttgaggctt
      901 ttattactta agctttttgc cgttttttcc gagcaggcgg tcaataacaa acgacggata
      961 accgagagcc aaaaacgccg ccgtaataaa gacaattata agcgcaacct tgtttttgcc
     1021 gtaaaggtct gtgtcgagaa acggatacgg gtaacggctg ctgccgccga agccggaaaa
     1081 gatcttgctg ccgaatttag cgcaaatcat tgtaaatgcg atatacagat agggcaaaat
     1141 cagccagcaa agcgggtgca aagccttgta taaccctttt gggaaaaaca gcagatagtc
     1201 gagtatcacc atgagaggaa cgatatagtg cataagaata tagcctgtgt aaaaagcctt
     1261 atcggaggcc gctgcctctt tggcaccgtt cggcataaac aaataggcta ttgcggtcac
     1321 ggctatacac atggttacgg cacctctgat aacggccttt tcctttttcg ggctgaaaac
     1381 ggcgagaaaa aagaagtata aaaagcagag tatgccgctt tgtaccgtgt agtaatataa
     1441 aagcgtgtcg gcgttgccgc gtcgcagact gtcaatatat tgtaaaatca tccctgcctc
     1501 acagcacaat aaaaaaatta tgcggtagag ctgtttcaaa ttctgacttt ttatatacat
     1561 aaaaaatgcc accccgaaaa actattgata taaactccat tttaacacaa taagtcgttt
     1621 gtatcaatgt atttcggagc ggcaaaagca aaatgtactt tggtgatttt agttatcggt
     1681 ctgtcttttt cgcttttagc ttttcctcga acggaatgca tttgctctcg tgattttcaa
     1741 tctttctgtt gagtcttgcg agagtgcttc caagctctgc gattctcttt ttgcgccctt
     1801 attcttcaaa agtttttgca acttgaggca taagctcgct taccttaatg tgctgcggac
     1861 acgctttttc acatttcctg cactttatgc agtccgaggc ttttccgtat tccttagcga
     1921 gattttcgta gtattggccg ttaaccgaaa aatctttata tgtagcgcgt ttttcctcgt
     1981 tataaagagc aaagtacttc ggtatcggaa tgtgcttcgg acagcctgcc gtgcagtagc
     2041 tgcagcctgt gcagggcacg gttatagaag cttttataat gtctactgcc ttgcctatga
     2101 tttcgaattc ttcctttgac aagggcttga agttttccat gtatccggtg ttgtcaagga
     2161 gctgttcgta tgtgctcatt ccgctgagta ccgtcataac gctgtcgtga cttgctgcaa
     2221 atcttaccgc ccaagaggga attgacgcgt ccggagcata atctttaagc agtttttcgg
     2281 cttcgtccgg tatttttgcg agtgttccgc cctttacagg ctccataaca attacctttt
     2341 tgccgtgctt taccgccgtt tcatagcata ggcgcgactg aatacccgga ttatcccaat
     2401 cgagatagtt tatctgaagc tgaactatct caatttcggg gtgcgcggtt aaaattctgt
     2461 c
//
LOCUS       DBMDVO010000143         2441 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20444_length_2441_cov_1.215812, whole genome shotgun sequence.
ACCESSION   DBMDVO010000143 DBMDVO010000000
VERSION     DBMDVO010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20444_length_2441_cov_1.215812"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="ACZPE2_02910"
     CDS             complement(<1..545)
                     /locus_tag="ACZPE2_02910"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318830.1"
                     /translation="MEAVKISNLSYKYPSADEYCLKNVSFSVSEGEVCAIVGPNGCGK
                     TTICNIIRGLIPSFHGGEVEGSVEIFGKSTKEISEEEKAKRIGFVFQNPFTQNSGIKE
                     TVFEEIAFGLENMGVDREEIITKVMDIISLLQIEAFMFKHPCELSGGQRQRVALASII
                     VQDPDIIVIDEPTSQLDPKGT"
     gene            complement(559..1344)
                     /locus_tag="ACZPE2_02915"
     CDS             complement(559..1344)
                     /locus_tag="ACZPE2_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014420.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIR1318831.1"
                     /translation="MDRQAIEEQLKRQGGTIKELSPITKFLFVICTAVLSILFGGPVY
                     IAVISAVYIILSLFAHVFKGFFKSYFKLFVFFGLMLILISSLFQNAEAEVLLSIGKIS
                     LYKVGFIRGCRMSSVILTLAGGLLLFTATTQVQDLMAVLGKMGLSSNVVYMVVASFRS
                     INELSDKMGQILEAQSSRGIETEGNLFVRLKAILPMLITLLLSSMVSAEEKAIALEAR
                     CFNCHCKRTSLRVNNTSRADVVTMIGIAFVTAALIAVKFIFIK"
     gene            complement(1374..2042)
                     /locus_tag="ACZPE2_02920"
     CDS             complement(1374..2042)
                     /locus_tag="ACZPE2_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1318832.1"
                     /translation="MKQKKSISIIVISLIPICLAINVVGGNLASALKIPLYVDSIGTI
                     LIGMLCGPAIGCLTGALTGVVSGIFSPIMFAYIPVTAIYGLAAGFLSKKKMMTSIPKL
                     ITSGIIIALLGVVLSSPITALVFGGITETGQGVIITFLRATGLGVLPATLISSFCTEF
                     LDKLLSCVVCYIIVKGMSNRYLSKFKLGHIYLKEKKEKNDLFDDDDIADENTDKNTDS
                     GEPQ"
     gene            complement(2089..>2441)
                     /locus_tag="ACZPE2_02925"
     CDS             complement(2089..>2441)
                     /locus_tag="ACZPE2_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318833.1"
                     /translation="NIESLEYYTSGWAIVWYVLDPDKMIILGSREVCGLNKILIDFKK
                     EYNIEKLPREINFGQLVYRDNKRFIEGIKYVYTPKGKLKMYKKILKILNTVKDLGGYA
                     CCHEIDRKLFMIGR"
BASE COUNT          696 a          573 c          450 g          722 t
ORIGIN      
        1 tccgttccct tcgggtcgag ctgagaggtc ggttcgtcta taacaataat atccgggtcc
       61 tgaacaatga tggaagccaa agcgactctc tgtctctgtc cgccggacag ctcacaggga
      121 tgcttgaaca taaaagcttc gatttggagc aagcttatga tgtccattac tttggttatg
      181 atctcttcac ggtcaactcc catattttca aggccgaaag caatctcttc aaaaacagtt
      241 tcttttattc cgctgttttg agtaaagggg ttttggaaaa cgaagcctat tcttttggcc
      301 ttttcttctt cgctgatctc ttttgtgctt tttccgaata tttcaaccga cccttcaacc
      361 tctccgccgt gaaaagacgg aataaggccg cggatgatat tgcaaattgt tgttttgccg
      421 cagccgttcg ggccgacaat cgcacatacc tctccctccg aaacagaaaa agaaacgttc
      481 ttcaggcagt attcgtcggc agatgggtat ttgtatgaca gattacttat tttaacagct
      541 tccatacttg tctccttatc atttgatgaa aatgaatttc acggcaataa gtgcagctgt
      601 tacaaaagct attcctatca tagtcacaac atctgccctg cttgtgttgt ttactcttaa
      661 agacgtgcgt ttgcaatggc agttaaagca tctcgcctct aaagctattg ctttttcctc
      721 cgcgcttacc atagagctga gcagcaatgt aataagcata ggtaaaatcg cctttaatct
      781 gacaaacaaa ttgccttccg tttcaattcc gcgagagctt tgagcttcaa gaatctgccc
      841 cattttgtcc gataattcgt ttatggagcg aaaggatgca actaccatat aaacaacatt
      901 ggacgacaga cccattttgc cgagaaccgc cataagatcc tgaacctgcg ttgtcgcggt
      961 aaagagcaaa agtcctcccg caagagtgag aattaccgaa gacatcctgc agcctcgtat
     1021 aaaaccgacc ttatataatg agatttttcc gattgaaaga agcacttcag cttcggcatt
     1081 ttgaaacaat gagcttatca aaataagcat aagtccaaag aaaacaaaaa gcttaaaata
     1141 cgatttaaaa aatcctttaa aaacgtgagc gaaaagagac agtattatgt ataccgcgct
     1201 gatcaccgca atatatacgg gtccgccgaa caatatcgat aaaacagccg tacaaataac
     1261 aaacagaaac tttgtaatcg gactaagctc tttaattgtg ccgccttgtc ttttcaattg
     1321 ttcttctatg gcctgtctat ccataaatct ccaccccggt caatgtattt ttcttattgc
     1381 ggctctccgc tgtccgtatt tttatccgta ttttcgtctg cgatatcgtc atcatcaaaa
     1441 aggtcatttt tctctttttt ctccttaaga taaatgtgac ctaatttgaa tttgctgaga
     1501 tatctgtttg acattccctt tacgataatg tagcaaacga cacacgaaag cagcttgtcc
     1561 aaaaattctg tacagaacga cgaaatcaat gttgccggga gcactccgag tccggttgcg
     1621 cgaaggaatg ttataattac tccttgcccg gtctcagtta ttccgccgaa caccagtgcg
     1681 gtgataggag acgacaatac tacgccaagc agcgcaataa taattccgct ggtaatgagt
     1741 ttcggtatgc tggtcatcat ttttttcttg ctcaagaagc cggcagccaa gccgtaaatg
     1801 gccgttacgg gaatatacgc aaacataatc ggtgaaaata ttcccgacac gacacctgtc
     1861 aaggcacccg taagacaacc tattgcaggt ccgcacagca taccgataag aatggttccg
     1921 atgctgtcaa catagagcgg tattttcaaa gcagaagcca gatttccgcc tacaacattt
     1981 attgccaagc atatcggtat caatgatatt acaataattg aaatagattt tttttgtttc
     2041 atatgtttct cccccgtgat atctcaccgg atcggaacaa aaacgctgct acctgccgat
     2101 cataaacaac tttctgtcta tttcatggca gcaagcatat cccccaaggt ccttcacggt
     2161 atttaatatc ttcagtattt ttttatacat cttgagcttt cccttaggag tgtaaacgta
     2221 ttttattccc tcgataaacc tcttattgtc ccgataaacc aactgtccga agttgatttc
     2281 tctcggtagc ttttcaatgt tatattcttt tttaaaatca atcagaattt tgttcagccc
     2341 acagacctct cgcgagccga gaattatcat tttatccgga tcaagcacgt accatacaat
     2401 tgcccaaccg gaagtataat actcaagcga ctcaatattt c
//
LOCUS       DBMDVO010000144         2439 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20474_length_2439_cov_1.291275, whole genome shotgun sequence.
ACCESSION   DBMDVO010000144 DBMDVO010000000
VERSION     DBMDVO010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20474_length_2439_cov_1.291275"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(71..649)
                     /gene="yihA"
                     /locus_tag="ACZPE2_02930"
     CDS             complement(71..649)
                     /gene="yihA"
                     /locus_tag="ACZPE2_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1318834.1"
                     /translation="MNLNKAEFEAAFGTSSQLPSSDLPEIAFAGRSNVGKSSLLNRLF
                     NRKALARVSSVPGKTATINFYKVDDARFVDLPGYGYAKVSKSEKIRWAEMLEGYFKTG
                     RNIRLVVQLIDMRHKPSADDLMMIDFLRSTGYPFVIVMTKSDKLNKTQFAERSAAIYD
                     EIPDSENVTIIPFSATNGLGREEITEYINNAL"
     gene            complement(660..>2439)
                     /gene="lon"
                     /locus_tag="ACZPE2_02935"
     CDS             complement(660..>2439)
                     /gene="lon"
                     /locus_tag="ACZPE2_02935"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1318835.1"
                     /translation="EEDINDRVQSQIDENQKEYYLHEQLKAINAELGEGDNPASEADG
                     YREKILKLHLDADSEKKLLNECDRLKYVQPSSPESAVSRNYLDKILELPWHIYSKENL
                     NIDNARRILDRDHYGLKDVKERILEFIAVRKLSPNIKGQIICLVGPPGVGKTSVAKSL
                     AKALNRKYERISLGGVHDEAEIRGHRKTYIGAMPGSIIEAVIRTKTSNPLILLDEIDK
                     LSNDYKGDPSSALLEALDPEQNNTFRDHYIEIPFDLSKVLFVTTANDKNEIPAPLLDR
                     MEVIELFSYTHEEKFNIAKKHLIPKQLKENGIKASQLHFTDNAIHSIIDGYTREAGVR
                     TLERTFAKVMRKAAVKITEGYTGKISVKPTGLETYLGPKKYKPESQDHKDEVGVVNGL
                     AWTSVGGEMLKVEAVKMPGTGKLELTGSLGDVMKESARTAYSYIRSISEALPLDMDFY
                     KNTDIHIHFPEGAVPKDGPSAGIAITTAVVSALTGIPVRADIAMTGEVTLTGNVLPIG
                     GLKEKTMAAYRNGIKTVLIPEENVSDLKEIDDAVKENVDFISVSKADEVLSLALSKNV
                     VVKDNKQRSVKLKQDRSQTASVCQQN"
BASE COUNT          614 a          569 c          461 g          795 t
ORIGIN      
        1 ccgccgacgg tcaaatggcc gctctcatta actcctaatc cgtctgatac aaacatttgg
       61 ttctctcctt ttaaagtgca ttatttatat actcggttat ctcttccctg cccaatccgt
      121 tagttgcaga aaaaggaata atagtaacat tttcagaatc cggtatttca tcgtaaattg
      181 ccgctgatct ttcggcaaac tgcgttttgt taagtttatc gctcttagtc ataacaataa
      241 caaacggata accggttgaa cgcaaaaagt caatcatcat aagatcgtct gcgctcggtt
      301 tgtgcctcat atcgataagc tgaacgacaa gtcttatgtt acgacccgtc ttaaaatagc
      361 cttcaagcat ttcagcccat cttattttct cggatttcga tacttttgca tatccgtatc
      421 caggtaaatc cacaaatctg gcatcgtcaa ccttgtagaa gtttatcgta gcggttttgc
      481 caggaactga gctcactctt gcaagtgcct ttctattaaa aaggcgatta agcaaagatg
      541 attttcccac atttgatcta cccgcaaaag ctatttccgg caaatcggaa gacggcagct
      601 gcgacgacgt tccaaatgca gcttcaaatt cagctttatt caaattcatt ttttacctct
      661 tagttctgtt ggcatacaga ggccgtttgg cttctgtcct gtttgagctt tacgctcctt
      721 tgtttattat ctttgacgac tacatttttg gatagcgcga gagacaatac ctcgtcggct
      781 tttgacaccg atatgaaatc gacattttct ttaacagcat catcgatttc tttcaaatcc
      841 gaaacatttt cctccggaat aagaacggtt ttaattccgt ttctgtatgc tgccattgtt
      901 ttttctttca atccgccgat cggcaataca ttaccggtca gagtaacttc acccgtcata
      961 gcaatatcgg ctcttaccgg tataccagtg agtgcggaaa caaccgcagt tgttattgct
     1021 attccggcag aagggccgtc tttaggaaca gctccctcgg ggaaatgaat atgaatgtcg
     1081 gtgtttttat aaaaatccat atcaagaggc aatgcttccg aaatactccg gatataactg
     1141 tatgccgtcc ttgcggattc tttcataaca tcgcccaaag aaccggtaag ctcaagcttt
     1201 cccgttcccg gcatcttaac tgcctcgacc ttcagcattt ctccgcctac agaagtccat
     1261 gcaagaccgt ttacaacacc gacttcatcc ttatggtcct gagattccgg cttatatttt
     1321 tttggcccga gataggtctc aaggccggtc ggcttcacag agatttttcc ggtatagcct
     1381 tcggttattt taacagcagc tttacgcatt acctttgcaa aagtgcgttc aagcgttctg
     1441 actccggctt ctcttgtata accgtctatt atactgtgta tggcattatc ggtaaagtga
     1501 agctgtgacg ctttgatacc gttttccttg agttgtttcg gtataagatg ttttttggct
     1561 atattgaact tttcctcgtg tgtgtaactg aaaagttcaa taacttccat tctatccaat
     1621 aagggagccg gtatttcgtt cttatcgtta gctgttgtaa caaaaagcac cttactcaaa
     1681 tcaaacggaa tttcaatata gtgatcacgg aatgtattgt tctgctcggg atcaagagct
     1741 tctaataaag cacttgacgg atcaccttta tagtcgttcg agagcttatc aatttcatca
     1801 agtaaaatca aaggatttga ggttttagtc ctgataacag cttcaattat actgcccggc
     1861 attgcaccga tataagtctt tctgtgacct cttatctctg cctcgtcgtg aacaccgccg
     1921 agagatattc tctcatactt tctgttaagc gcctttgcaa gagactttgc aacagaggtt
     1981 ttgccgacgc ccggagggcc gacaagacat attatctgcc ccttaatatt tggcgacagt
     2041 tttctcacag caataaattc aagaattcgc tctttaacgt ctttaagtcc gtaatgatct
     2101 ctgtcaagta ttctccgagc attatcgata ttaagatttt cttttgagta aatatgccac
     2161 ggaagctcca aaatcttatc aagataattt cttgatactg cactttcggg gctgctcggc
     2221 tgcacatatt taagcctgtc gcactcgttc agcagcttct tttcgctgtc ggcatcaaga
     2281 tgcagcttga gtattttttc gcgatagccg tcagcctcgg aagctggatt gtctccttcg
     2341 ccgagttcgg cgttaatagc ctttaactgc tcatgaaggt aatattcctt ttgattttca
     2401 tctatttggc tctgtactcg gtcatttata tcctcttca
//
LOCUS       DBMDVO010000145         2434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20538_length_2434_cov_1.354051, whole genome shotgun sequence.
ACCESSION   DBMDVO010000145 DBMDVO010000000
VERSION     DBMDVO010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20538_length_2434_cov_1.354051"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="ACZPE2_02940"
     CDS             complement(<1..345)
                     /locus_tag="ACZPE2_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1318836.1"
                     /translation="MKILVTGSAGFVGKNLVEALKNLRDNKDRTRPSLSIDEIFEYDV
                     DTDKALLSDFCRECDFVFNLAGVNRPKDDGEFMKGNFGFASELLKTLEKHGNRCPVML
                     SSSIQATLVGRYA"
     gene            complement(373..1416)
                     /locus_tag="ACZPE2_02945"
     CDS             complement(373..1416)
                     /locus_tag="ACZPE2_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1318837.1"
                     /translation="MSVFNGKTLMITGGTGSFGNAVLNRFLQTDIGEIRIFSRDEKKQ
                     DDMRHEFQAKMPEVSDKIKFFIGDVRDLASVKNAIHGVDYIFHAAALKQVPSCEFFPI
                     EAVKTNVLGTDNVLTAAIDEGVKTVICLSTDKAAYPVNAMGTSKAMMEKVIVAKSRTV
                     SPDKTKICCTRYGNVMCSRGSVIPLWIEQIKEGKPITVTEPNMTRFIMSLDEAVDLVL
                     FAFQNGVSGDILVQKAPACTIGVLAEAVKELFCPEAEIKVIGIRHGEKMYETLLTNEE
                     CAHATDMGNFYRVPCDKRSLNYDKYFNDGDKDRNPLTEFNSNNTELLNVEQVKEKLLS
                     LAYIRDELAEFKK"
     gene            complement(1423..>2434)
                     /locus_tag="ACZPE2_02950"
     CDS             complement(1423..>2434)
                     /locus_tag="ACZPE2_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1318838.1"
                     /translation="PMLTAEQVDSTYNTLYYNGYNILYKGKLKIKPKFKNPDISFIKE
                     TVTYSAFIFLQMIATQINSMTDTILLGILAKNASAIIAIYGVGSQIIQYFKTIGGHFN
                     GVLMAGVVRMVENGAKVKDLQNEMVRIGRIILMMLGMIFVVFIAFGKHFMILWAGDAY
                     EQSYYVAIAIMLPTMFTLVQSIGSQILWAMNRHKGQAIIQIISALINIVLTAVLISWK
                     PLEGAVIGSVIALTVGDVLCMNVMFKKKIGIKLGEYYYGLLKGVLPSLLICFVGGKLF
                     SLIGLEKYGWLGFVVNCVVMIVIYGICMLVFGMNKSEKALAFGLLNKLLRKTNLVKDK
                     GV"
BASE COUNT          703 a          577 c          448 g          706 t
ORIGIN      
        1 tgcatatctg ccgacaagcg tcgcctgtat ggagcttgag agcataacgg gacaacggtt
       61 accgtgcttt tcaagagttt tcaaaagctc cgaagcgaag ccgaaattcc ctttcataaa
      121 ctcgccgtcg tccttcggcc tgtttactcc cgcgagattg aaaacaaaat cgcactccct
      181 gcaaaaatcg gagagcagag ccttatccgt atcaacatca tattcaaata tttcgtcaat
      241 cgagagcgac ggacgggtac ggtctttgtt atcgcgaaga tttttaagtg cctcgacaag
      301 gtttttgcct acaaatccgg ctgaacccgt aacaagtatt ttcataaaaa agctgccttt
      361 cggaatacaa aatcacttct tgaattcggc taattcgtct ctgatataag cgagcgataa
      421 aagcttttcc tttacctgct caacattgag aagttctgta ttatttgaat tgaactctgt
      481 caacggattg cggtctttat cgccgtcatt gaaatattta tcgtagttaa gtgaacgctt
      541 atcgcaaggc acacggtaga aattgcccat atcggtagcg tgagcgcact cctcgttagt
      601 aaggagggtt tcgtacatct tttcgccgtg acgaataccg ataaccttaa tttcggcttc
      661 ggggcagaaa agctccttta ccgcctcggc aagcacaccg attgtgcaag ccggcgcttt
      721 ctgaacaaga atatcgcccg acactccgtt ttggaatgca aagagaacga ggtcaaccgc
      781 ctcgtcgaga gacataataa atcgtgtcat attcggctct gtaacggtta tcggtttgcc
      841 ctctttaatc tgttctatcc aaagcggaat taccgagccg cgcgagcaca taacgtttcc
      901 gtaacgagtg cagcaaatct tagtcttgtc gggacttacg gttcttgatt ttgcgactat
      961 taccttttcc atcatagcct ttgacgttcc cattgcgttt acgggatatg ccgccttgtc
     1021 ggttgacagg cagattacgg ttttaacgcc ctcgtcaata gctgccgtaa gcacgttatc
     1081 tgtgccgaga acattggtct taaccgcttc gatggggaaa aattcgcaag acggaacctg
     1141 ctttaaggcc gccgcatgga agatatagtc tacaccgtgt attgcgtttt taacggacgc
     1201 caagtctctg acatcgccta taaagaattt gattttatcg ctgacctcgg gcattttcgc
     1261 ctgaaactca tggcgcatat cgtcctgctt tttttcatct cttgaaaaaa tgcggatttc
     1321 gccgatgtcg gtctgcagaa atctgttgag caccgcattg ccaaatgagc ctgttccgcc
     1381 cgtaatcata agtgtttttc cgttgaatac tgacattacc gtttatactc ctttatcttt
     1441 tacaagattt gtttttctca ataatttatt taacaatcca aaggctaaag ccttttcaga
     1501 tttattcata ccgaacacca acatacatat accgtaaatt acaatcataa ccacgcaatt
     1561 tacaacaaat ccaagccagc cgtatttttc aagaccaata agtgagaaca atttcccgcc
     1621 gacaaagcaa atcaacaatg acggcaatac acctttaagc agtccataat aatactcgcc
     1681 aagctttatt ccgatttttt tcttaaacat tacattcata cacaatacat cgcccacagt
     1741 caatgcaatc acagaaccta taaccgcacc ctcaagaggt ttccagctta tcaaaacggc
     1801 tgttaagaca atgtttatca atgcggaaat aatctgtatt atagcctgcc ccttgtgtct
     1861 gttcatagcc caaagaattt gagagcctat tgactgaaca agcgtaaaca tagtaggcag
     1921 cataattgca atcgcaacat agtatgattg ctcataagca tctcccgccc acaatatcat
     1981 aaaatgttta ccgaaagcga taaacacaac aaaaatcata ccaagcatca taagtattat
     2041 tctgccgata cgaaccattt cattttgaag atccttaact tttgcaccgt tttcaaccat
     2101 acgcacaacg cccgccataa gcactccgtt gaaatgaccg ccgattgttt taaaatactg
     2161 aattatctgc gaaccgactc cgtaaatcgc aataattgcc gatgcatttt ttgctaaaat
     2221 accgagtaaa atggtatctg tcattgaatt tatctgcgtt gctatcatct gcaaaaaaat
     2281 aaatgccgaa tatgtaacag tttcctttat aaatgaaata tccggatttt tgaacttggg
     2341 ttttattttt aatttcccct tgtacagaat attataaccg ttataataca aagtattata
     2401 cgtagaatcc acctgctctg cagtcagcat cggg
//
LOCUS       DBMDVO010000146         2428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20615_length_2428_cov_1.324022, whole genome shotgun sequence.
ACCESSION   DBMDVO010000146 DBMDVO010000000
VERSION     DBMDVO010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20615_length_2428_cov_1.324022"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="ACZPE2_02955"
     CDS             complement(<1..284)
                     /locus_tag="ACZPE2_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR1318839.1"
                     /translation="MKFSKATKLSAIIMTFVLVLGLVACSADKTEKKKSNVDIKTLMA
                     QEPNDADEAAKLYDKLLQAENDILSNNSALWEKVFLSANKNTPMIEDGTN"
     gene            complement(307..1239)
                     /locus_tag="ACZPE2_02960"
     CDS             complement(307..1239)
                     /locus_tag="ACZPE2_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1318840.1"
                     /translation="MDKEKEKKKKKPSHLLTRYREFVQAAATLATNIHLPNFLKGSIY
                     TGGGKAVCVPGLNCYSCPAATGACPIGSFQAVAGSSKFRFSYYIAGFLILFGVLLGRF
                     ICGFLCPFGWFQDLLHKIPCKKLSTEKLKPLTYLKYAVLLFAVVLLPALIVNDVGMGD
                     PFFCKYICPQGVLEGAIPLAAVNEGIRSALGSLFTWKLAVLIAIIFLSILFYRPFCKW
                     LCPLGAFYALMNKVSLFGIKVDEHKCVSCGKCAGACKMDVDITKTPDHAECIRCGKCI
                     SVCPTEAVSFRYGFNLPKTLSSGNEKPKNDKKSK"
     gene            complement(1232..1396)
                     /locus_tag="ACZPE2_02965"
     CDS             complement(1232..1396)
                     /locus_tag="ACZPE2_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HIR1318841.1"
                     /translation="MKGANGLLRAKKAVIQCVMLLAGAAMVCVGAMRGEADTVLAKAI
                     RLCLECVGIG"
     gene            1768..>2428
                     /locus_tag="ACZPE2_02970"
     CDS             1768..>2428
                     /locus_tag="ACZPE2_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1318842.1"
                     /translation="MHILVVEDEKALCDTIARSLRRLAYSVDCCYDGQSALDMLSIEK
                     FDLVVLDLNLTEKDGLTVLKTLRKDDKDTKVLILSARCEVADKVNGLDAGANDYLTKP
                     FHLDELAARIRSLTLRCFTQNDVVLSCGNLLFDTKLRKASVSGDNLTLTRKETEILEY
                     LILNQGRPISQEEFLEHVWDSSIDDFSNSIRVHISSLRKKLRAALGYDPIHNRIGEGY
                     VM"
BASE COUNT          678 a          599 c          498 g          653 t
ORIGIN      
        1 tagtttgtgc cgtcctcaat catcggcgta tttttatttg ccgataaaaa cactttttcc
       61 caaagagccg aattattcga gagaatatcg ttttcggctt gcaaaagctt atcgtaaagc
      121 ttcgcagcct cgtctgcgtc attcggctct tgtgccatta aagttttaat atcgacattg
      181 ctttttttct tctccgtttt gtcggcggag cacgccacaa ggcccagcac aagcacaaat
      241 gtcataataa ttgctgaaag tttggttgct tttgaaaatt tcacagttat tcctccgttt
      301 tcttacctat tttgattttt tatcattttt cggcttttca ttgccgcttg agagtgtttt
      361 cggcaaattg aaaccgtatc gaaagcttac cgcctccgtc ggacagacac tgatgcactt
      421 gccgcaacgt atacattctg cgtggtcggg cgtttttgta atatcaacgt ccattttgca
      481 cgcgcctgcg cattttccgc aggagacaca tttatgctca tcaaccttta ttccgaacag
      541 cgacaccttg ttcatcagcg cataaaacgc gccgagcgga caaagccatt tgcaaaacgg
      601 gcggtaaaac agtatgctca aaaatatgat tgcaattaaa accgccagct tccatgtaaa
      661 cagcgagcct aaagccgaac gtattccctc gttgactgcc gcgagaggaa ttgcaccctc
      721 aagcacaccc tgcggacaaa tatacttgca aaaaaacggg tcacccattc cgacatcatt
      781 tacaatcagt gccggaagca atacgaccgc aaacaacagc accgcatatt ttaaatatgt
      841 caatggtttc agtttttcgg ttgacagctt tttgcacggg attttatgca gtaaatcctg
      901 aaaccatccg aacgggcaga gaaatccgca tataaatctg cccaaaagca cgccgaataa
      961 tatcaaaaag cccgctatgt aatacgaaaa ccgaaacttt gacgaacctg ccactgcctg
     1021 aaacgagcct atcggacacg ctccggttgc cgcaggacag gaataacagt taagccccgg
     1081 tacgcaaacc gccttgccgc cgcccgtata aatgctgccc tttaaaaaat tcggcaggtg
     1141 tatgtttgtt gcgagcgttg ccgccgcctg cacaaattct ctgtatcggg tcagtaaatg
     1201 cgacggcttt ttctttttct tttccttttc cttatccaat tcccacacac tccaaacaca
     1261 atcttatcgc ttttgcaagt acggtatccg cttcgcctct catagcaccg acgcacacca
     1321 tcgccgcccc tgcaagcagc ataacacatt gaattacggc tttttttgcc cttaacaaac
     1381 cgttcgctcc cttcattttt gcctcattat atcacggcgg gcataaattc tctgttaaga
     1441 attgaaattt aacagagatt ttatacttta tgttataata acctcacgaa acgaaaacaa
     1501 aatcagagat tttgaccgtt tcggagaaca ttgaatgact aaattgcggc tttcgccgct
     1561 gcacttcgtg cgctcctgct gtcgcagggg aatttataat aaaataacct cacgaaacga
     1621 aaacaaaatc aaagattttg accgtttcgg agaacattga atgactaaat tgcggctttc
     1681 gccgctgcac ttcgtgccgc tcctgctgtc gcaggggaat ttatgataaa atacttataa
     1741 ttgaaaaaac agacaggaga atcgcgtatg cacattttag ttgtagaaga cgaaaaggct
     1801 ctttgcgaca ccatcgcacg cagtttaagg cgtctcgctt acagtgtaga ctgctgttac
     1861 gacgggcaaa gtgctttgga tatgctctcg atagagaaat tcgaccttgt tgtattggac
     1921 cttaatctca ccgaaaaaga cggtttaacc gttcttaaaa cactcaggaa agatgataaa
     1981 gacacaaaag tgctgatttt gtcggctcgg tgcgaagttg ccgacaaggt taacggactt
     2041 gacgcaggag caaacgacta tctgacaaag ccgtttcatt tggacgagct ggcggcacga
     2101 atccgcagtc tgacgctccg ctgtttcact caaaacgatg ttgtcttgtc ctgcggcaat
     2161 ctcctatttg atactaaatt gagaaaggcg tccgtttcgg gcgataacct gacgctcaca
     2221 agaaaagaaa ccgaaattct cgaatacctc atactcaacc aggggcgtcc gataagtcag
     2281 gaggaatttc ttgaacacgt ttgggacagc agtatagacg acttcagcaa ttccataaga
     2341 gttcacatct catcgctcag aaaaaagctt cgcgccgctt tgggatacga cccgatacat
     2401 aatcgcatag gggaaggcta cgtgatgg
//
LOCUS       DBMDVO010000147         2411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20804_length_2411_cov_1.040693, whole genome shotgun sequence.
ACCESSION   DBMDVO010000147 DBMDVO010000000
VERSION     DBMDVO010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20804_length_2411_cov_1.040693"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="ACZPE2_02975"
     CDS             <1..326
                     /locus_tag="ACZPE2_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1318843.1"
                     /translation="VVGDGAKVVSQTPAAGQQMRSGGVIVVYTDSSSQKAVATVPNLC
                     NMSISAANKAAVNAGFNIKVAGNSSSNDFVSYKQSIAEGTEAEQGSVITVYFKSSVNV
                     QDGPA"
     gene            358..>1036
                     /locus_tag="ACZPE2_02980"
     CDS             358..>1036
                     /locus_tag="ACZPE2_02980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1318844.1"
                     /translation="MLLSELLKGIDTKSNFNDTEITDITDNTAKLCEGCVFVCIKGAH
                     FDGHSAAEEAVNKGARAVVAEYDTGVENQILVKNTRDAYSLLCANFYGNPSEKLHLIG
                     ITGTNGKTTTTFILKGIFDNLGIKSGLIGTVKNVTGDKEYPSSLTTPDSKELQRLFRE
                     MVDSGCKYCVMEVSSQALAQGRVDGCHFDIALFTNLTQDHLDYHKTFENYRNAKRKLF
                     EIADTAVI"
     assembly_gap    1037..1046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1047..1790
                     /locus_tag="ACZPE2_02985"
     CDS             <1047..1790
                     /locus_tag="ACZPE2_02985"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1318845.1"
                     /translation="HYMVDGLPIKVVTFSVKNNEAQFVAKNVVCTSSSVQYELLWNDK
                     IGRANVGIPGHFTVYNTMGAAICAILTGLDFSDVLSSLKKSKGVPGRVEVVPTDTDYT
                     VLIDYAHSPDGLENVISSVRETTNGRIIAVFGCGGDRDKTKRPKMGKIVGDLADVAVV
                     TSDNPRSENPQLIVDDVLEGMHNCKAKIKVIVDRTEAIRYALGEAKKGDVVLLAGKGH
                     ETYQILNTGKIHYDEREVVAGILAEGKDK"
     gene            1790..>2411
                     /locus_tag="ACZPE2_02990"
     CDS             1790..>2411
                     /locus_tag="ACZPE2_02990"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1318846.1"
                     /translation="MELTLSRIAKAVGGRLSGYDCIVTSVSTDSRDIPKGCLFICLEG
                     EKFDGHDFVSQAAQNGAAAVLVRKKVDSRIPVITVKDTGKALLDLAGYYRGTLDNLTV
                     VGLTGSVGKTTTKEMTYAVLAEQFKTIKTEGNLNNEIGMPKTLLRLKDDTEAAVIEMG
                     MNHFGEIERMTKSSRPDIGIITNIGVSHIEYLGSRDGILKAKLEILK"
BASE COUNT          707 a          517 c          606 g          571 t
ORIGIN      
        1 aggttgtcgg agacggtgct aaggtagttt cacagacgcc tgcggcaggc caacaaatga
       61 ggtcgggcgg agtaatagtg gtatataccg attcttcgtc gcaaaaggca gttgccaccg
      121 ttccgaatct ttgcaatatg tcaatcagcg cagccaataa ggccgctgtt aacgcaggct
      181 ttaacataaa ggtagccggc aattcttctt caaatgactt tgtgtcttat aagcagagca
      241 ttgcggaggg caccgaggca gagcagggct ctgtaattac agtttacttc aagtcgtccg
      301 taaacgtaca ggacggtccc gcataatatt aaagtaaaaa acaggaggaa aaaaccgatg
      361 ctgttatcag agcttttaaa gggcattgat acaaaaagca attttaatga cacggaaatt
      421 actgatatta ccgataatac cgcaaagctt tgcgagggtt gcgtttttgt gtgcatcaag
      481 ggcgcacatt ttgacggcca ctctgccgcc gaggaggctg tgaataaagg agcccgtgcg
      541 gtagtggcgg agtatgatac cggtgttgaa aatcagattt tggttaagaa cacaagagat
      601 gcatattctt tattgtgtgc caatttttac ggtaatccgt ccgagaagct tcatcttata
      661 ggcattaccg gaacaaacgg aaagactaca acaaccttta ttttaaaagg aattttcgat
      721 aatctcggaa taaaatccgg acttataggc acggttaaaa acgtaacggg cgacaaagag
      781 tacccgtcgt cactcaccac tcccgacagc aaagagcttc agagactctt cagggaaatg
      841 gttgacagcg gctgtaaata ctgcgttatg gaggtctctt cccaagcact cgcgcagggg
      901 agggtagacg gatgtcattt cgacatagca ttgtttacca atctcacgca ggatcacctt
      961 gactatcaca aaacctttga aaactatcga aacgccaagc gcaagctgtt tgaaatagcg
     1021 gacacggctg ttataannnn nnnnnncatt atatggtaga cggactcccg ataaaggtcg
     1081 tgacattttc ggttaaaaac aatgaggcgc agtttgtggc aaaaaatgtt gtctgtacct
     1141 catcctctgt gcagtacgaa ctcctttgga acgataaaat cggcagagca aacgtaggca
     1201 tacccggtca cttcaccgtt tacaatacga tgggtgccgc aatttgcgcc atactcacgg
     1261 gacttgattt ttccgatgtc ctttcttcgc tcaaaaagag caagggtgtg cccggcagag
     1321 ttgaggtagt tccgacagat acggattata ccgttcttat agattacgca cattctcccg
     1381 acggccttga aaatgtaata tcatcggtcc gtgaaaccac aaacggcaga attatagccg
     1441 tattcggctg cggcggcgac agagacaaga ccaaacgccc taaaatgggc aagatagtag
     1501 gcgaccttgc agatgttgcg gttgtaacct ctgataatcc gcgaagcgaa aacccgcagc
     1561 ttatagttga cgatgtttta gagggtatgc ataactgcaa ggcgaaaatc aaggttattg
     1621 ttgaccgtac cgaggccata agatatgcac tcggcgaagc gaagaagggc gatgtcgttc
     1681 tccttgccgg caaagggcac gagacgtatc aaatattaaa taccggcaaa atacactatg
     1741 atgaacgtga agttgtggca ggtatattag cagaaggaaa agataagtaa tggaattaac
     1801 tctttcgcgt attgcaaaag ccgtaggcgg caggctctcg ggctatgatt gtattgtgac
     1861 atcggtttcg accgacagcc gcgacatacc aaaggggtgc cttttcatat gccttgaggg
     1921 agaaaaattt gacgggcatg actttgtgtc gcaggcggct caaaacggag ctgcggcggt
     1981 gcttgtccgt aaaaaggttg acagccgcat acccgtaatc acagttaagg ataccggcaa
     2041 ggctctgctt gaccttgcgg ggtattaccg cggtacgctt gataatttga cggtagtagg
     2101 ccttaccggc tcggttggca aaacgacgac aaaagaaatg acctacgccg tgcttgccga
     2161 gcagtttaaa acgataaaga ccgagggcaa tctaaataat gaaatcggaa tgcccaaaac
     2221 tctgcttcgc cttaaagatg ataccgaggc ggcggttatt gaaatgggta tgaatcattt
     2281 cggcgaaatc gagagaatga caaaaagctc tcgtcccgac atcggtataa taaccaatat
     2341 cggcgtttcg catatagagt atctcggctc acgtgacgga atcctaaaag cgaagcttga
     2401 aattctcaag g
//
LOCUS       DBMDVO010000148         2408 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20838_length_2408_cov_1.302124, whole genome shotgun sequence.
ACCESSION   DBMDVO010000148 DBMDVO010000000
VERSION     DBMDVO010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20838_length_2408_cov_1.302124"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /gene="glyA"
                     /locus_tag="ACZPE2_02995"
     CDS             complement(<1..363)
                     /gene="glyA"
                     /locus_tag="ACZPE2_02995"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678738.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1318847.1"
                     /translation="MYADMMDTIGFVSKYDEEVGKAMNLELARQQRNIELIASENIVS
                     PAVMAAMGSVLTNKYAEGYPGKRYYGGCEDVDIVENIARDRAKKLFGAKFANVQPHSG
                     AQANIAVYFALLNPGDTVM"
     gene            complement(403..1869)
                     /gene="guaB"
                     /locus_tag="ACZPE2_03000"
     CDS             complement(403..1869)
                     /gene="guaB"
                     /locus_tag="ACZPE2_03000"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1318848.1"
                     /translation="MATDEKFAKEGLTFDDVLLIPAESDVLPKDIDVSTRLSKNITLN
                     TPVLTAAMDTVTETRMAIAIAREGGVGIIHKNMSIEAQKSEVDKVKRSENGVIVNPFF
                     LHPQNTVKEADELMHRYRISGVPICDDSGKLVGILTNRDMRFMSDYNVEIAGVMTKDH
                     LVTSHIGTTLDEAKQILMRHKIEKLPLVDENGYLKGLITIKDIEKSVQYPNSARDSRG
                     RLLCGAALGATSDVLDRAAALAEAGVDVLTLDSAHGHSHNILKSVAKVKAAFPNITLI
                     AGNVATAEGTKALIDAGADCVKVGIGPGSICTTRVVAGIGVPQITAIYDSANEASKHG
                     VSIIADGGIKYSGEIVKAIAAGASAVMVGSLVAGCEESPGDSEIYQGRRFKVYRGMGS
                     IAAMNNGSKDRYFQEDNKKLVPEGIEGRVPYKGTLSDTIFQMMGGLKSGMGYCGCKNI
                     TELQTKTKFIRITNAGLKESHPHDVFITKEAPNYSVNL"
     gene            complement(2039..>2408)
                     /locus_tag="ACZPE2_03005"
     CDS             complement(2039..>2408)
                     /locus_tag="ACZPE2_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1318849.1"
                     /translation="PPGTGDVPLTVFQSLPVDGIIIVRTPQDLVTMIVKKAYNMAKMM
                     NVPIIGLVENMSYFECPDCKKKYNIFGESKIDEVAKELGVPVLARLPIDPKTASLVDK
                     GAVELCDDKAIAPIIDKLPK"
BASE COUNT          536 a          626 c          556 g          690 t
ORIGIN      
        1 cattacggta tcgccgggat taagaagagc aaagtataca gctatattcg cctgagcacc
       61 cgaatgaggc tgaacatttg cgaatttggc accgaaaagc ttcttagctc tgtcgcgcgc
      121 gatgttttcc actatatcaa catcctcgca gccaccgtag tagcgcttgc cgggatagcc
      181 ctcggcgtac ttgtttgtaa gaacgctgcc catggccgcc ataactgccg gcgaaacgat
      241 gttttcgctc gctataagct ctatgttcct ctgctgacgg gcaagctcca aattcatagc
      301 cttgcccact tcttcatcat actttgatac gaagcctatc gtatccatca tatctgcgta
      361 catttttttc tccttatttc tttatttcac gctgcttgcg aattataaat taacggaata
      421 gttgggagcc tcttttgtta tgaatacatc gtgcggatgg ctctctttaa gacctgcgtt
      481 ggttattctt atgaacttcg tcttagtctg aagctcggtt atatttttgc agccgcagta
      541 gcccatacct gatttaagac cgcccatcat ctggaagatt gtatcggaga gcgttccctt
      601 ataaggaact ctgccctcaa ttccctcggg aacaagcttc ttgttgtcct cctggaaata
      661 tctgtcctta gagccgttgt tcatggcggc tatactgccc attccgcggt aaaccttgaa
      721 tcgtctgccc tgataaattt cgctgtctcc gggggactcc tcacagcctg cgacaagcga
      781 acctaccata accgccgaag cacctgcggc gatagccttg actatttcgc ccgaatactt
      841 gatgccgccg tcggcaatta ttgaaacgcc gtgctttgaa gcctcgtttg cgctgtcata
      901 gattgccgtt atctgcggaa cgccgatacc ggcgactacg cgcgttgtgc atattgagcc
      961 ggggcctata cctactttga cgcagtctgc gccggcgtcg ataagtgcct ttgtgccctc
     1021 tgccgtagct acgttaccgg caataagcgt gatattcggg aaagccgcct ttaccttggc
     1081 gacggatttc aatatgttat ggctgtggcc gtgagccgag tcaagcgtga gaacgtcaac
     1141 gccggcttcg gcgagagccg cagcgcggtc aagaacgtcg cttgtggcac cgagagccgc
     1201 accgcagaga agtctgccgc gagaatccct tgcggaattc gggtactgaa cgctcttttc
     1261 aatatcttta atggtgataa gtccctttaa gtagccgttt tcatctacaa gcgggagctt
     1321 ttctatcttg tggcgcatca aaatctgctt tgcctcgtca agcgttgttc cgatgtgaga
     1381 ggtgacgaga tggtctttcg tcataacgcc tgcaatctcc acattatagt cggacataaa
     1441 acgcatatcg cggtttgtga gtatgcctac aagcttgccg ctgtcgtcac agatgggaac
     1501 acccgaaatt ctgtaacggt gcataagctc gtccgcttcc tttaccgtgt tctgcggatg
     1561 caggaaaaac ggatttacta taactccgtt ctcacttctc ttaaccttgt ccacctcact
     1621 cttttgagcc tcaatggaca tattcttgtg aattatgcct acgccgccct cacgcgctat
     1681 ggctattgcc attctcgttt cggttacggt gtccatggcc gccgttaata ccggcgtgtt
     1741 gagcgttatg ttctttgaaa ggcgggttga aacgtcaata tccttgggga gcacgtcgct
     1801 ctcggccggg ataagcaaaa cgtcatcaaa ggtcaggccc tcttttgcaa atttctcatc
     1861 tgttgccata ctgatattac ctcccattat agttagtatt attataacat ctaatacaat
     1921 catagttcaa tacttaatat tgaaaaaaca cccgcaaaga gtacgggtgt ttttgtagat
     1981 gttgtcttat gtccggacaa agcgtcgcat ttcggcaaag aatacagtat tctgccgctt
     2041 atttcggaag cttgtcgatt atgggagcta tcgccttgtc gtcgcagagc tccaccgcgc
     2101 ccttatccac aagcgaagcg gttttggggt ctatcggcag acgtgcgagc accggaacgc
     2161 cgagctcctt tgccacctcg tctattttgc tctcaccgaa tatattgtat ttctttttgc
     2221 agtcggggca ctcaaaatag ctcatatttt cgacgagtcc gattatcgga acattcatca
     2281 tctttgccat attgtatgcc tttttgacta tcatggtaac aagatcctgc ggagttctga
     2341 caattataat accgtctacg ggaagactct ggaataccgt cagcggaacg tcgcccgtgc
     2401 cggggggc
//
LOCUS       DBMDVO010000149         2401 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_20920_length_2401_cov_1.203913, whole genome shotgun sequence.
ACCESSION   DBMDVO010000149 DBMDVO010000000
VERSION     DBMDVO010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20920_length_2401_cov_1.203913"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="ACZPE2_03010"
     CDS             complement(<1..712)
                     /locus_tag="ACZPE2_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318850.1"
                     /translation="MAKCPKCGAKLHWYDFRAECKHCGVNIPNYKWEERLENDADTAE
                     TSFAKLHYKMSNFKYGTIGSPLMIVRLVFTLLPLVAFVVPLVKITLCFPFFEKTESVS
                     FLTFILNYLTEFDIKASLSLMQGEALGGVFAKMFLGVGFAFLAAALGVVNFFVVLIAS
                     MSLSSKADTVLNILSTVFWSLSAYFVYAFLNAAAETSVNIITGSVSPFFAVGIALFLA
                     NVIINIAVSKKFKNKKATD"
     gene            complement(731..2230)
                     /gene="arfA"
                     /locus_tag="ACZPE2_03015"
     CDS             complement(731..2230)
                     /gene="arfA"
                     /locus_tag="ACZPE2_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosylfuranosidase ArfA"
                     /protein_id="HIR1318851.1"
                     /translation="MKKAELSLAGEFKVGKVDDRIFGSFIEHLGRAVYGGIYEPHHPC
                     ADEDGFRKDVIDEVKKLRVPIVRYPGGNFVSGYRWEDGVGPISERPKRLELAWGVTEP
                     NLFGTDEFMKWCKKADTACMMAVNLGLRGVDDARNLVEYCNHPSGSYYSDMRRKNGAE
                     SPYGIKLWCLGNEMDGGWQLGHKEAKEYAFLADQASKAMKLTDDSIETVICGSSNDHV
                     KTFGKWEDTCLDIAYDSVDYISLHQYYDNKLGDTQSFLAKSMAMDEFIKTVICICDSV
                     KGRKHSKHTVNLSFDEWNVWFHSNGDEVEKWSTAPHQLEDVYTFEDALLVGLMLITLL
                     KHADRVKVACLAQLVNVIAPIMTENGGGIFEQTIFYPFMHASNYGRGTVLLSNTVCGK
                     HDTREFTDVPDVDSVAVLSDDGNALTVFAVNRDPSESYDMCIKLLDLEDFSGKEHIEM
                     CGFDKNAVNGFDKINVFPVSGEIPTVDGKTAHAVLKPFSWNVIRFYKNK"
BASE COUNT          579 a          593 c          535 g          694 t
ORIGIN      
        1 gatctgtggc ttttttgttt ttgaattttt tggacaccgc aatattgatt atgacatttg
       61 cgaggaacaa cgctattcct accgcaaaga acggggatac cgagcctgtg attatgttta
      121 cgcttgtttc ggccgcggca tttaaaaagg cgtaaacaaa gtatgccgaa agactccaaa
      181 aaacggtgct gaggatgttt aataccgtgt cggctttgct gctgagtgac attgacgcta
      241 taagcacaac gaagaaattt acaactccga gcgcggcggc caaaaacgca aatcccacgc
      301 cgagaaacat ttttgcaaaa acaccgccga gtgcctcgcc ttgcatcagc gagagcgaag
      361 cctttatatc aaattcagtc aaatagttaa gtataaacgt gagaaatgaa acactctccg
      421 ttttttcgaa aaacgggaag cagagcgtga tttttacaag cggcacaaca aaagccacaa
      481 gcggcaaaag agtaaaaaca aggcgcacta tcataagcgg agagcctatc gtgccgtatt
      541 tgaagttact cattttgtaa tgcagctttg cgaatgatgt ctcggccgta tcggcgtcat
      601 tttcaagcct ctcttcccat ttgtagttgg ggatattaac tccgcagtgt ttacattcgg
      661 cacggaagtc gtaccaatga agctttgctc cgcacttggg acattttgcc ataattctca
      721 ttccttttta ttatttgttt ttataaaatc ttataacatt ccatgaaaac ggctttaaaa
      781 cggcgtgtgc cgtcttgccg tcaaccgtgg ggatttctcc gcttacgggg aatacgttta
      841 ttttgtcgaa tccgttgacc gcgtttttgt caaagccgca catttcaata tgttccttgc
      901 ccgaaaagtc ttcaagatcc aaaagcttta tgcacatatc atagctttcg ctcgggtctc
      961 tgtttaccgc aaatacggta agagcgttgc cgtcgtcact taaaaccgcc acggagtcca
     1021 cgtccggaac atcggtaaac tctcttgtgt cgtgcttgcc gcatactgtg tttgagagta
     1081 aaacggttcc tctgccgtaa tttgacgcgt gcataaacgg atagaatata gtctgctcaa
     1141 aaataccgcc gccgttttcc gtcataatgg gtgctatgac gtttacaagc tgtgcaagac
     1201 acgcgacctt caccctgtcg gcatgcttta acagagttat cagcataagc ccgaccaaaa
     1261 gtgcgtcctc aaaggtgtat acgtcttcaa gctggtgcgg agcggtgctc cacttctcta
     1321 cctcgtcgcc gtttgagtgg aaccaaacgt tccactcgtc aaaggagaga ttgacggtgt
     1381 gctttgaatg ctttctgccc tttacggaat cgcatataca aattaccgtt tttatgaatt
     1441 cgtccatagc catgctctta gcgaggaagc tctgtgtgtc gccgagcttg ttgtcgtagt
     1501 attgatgaag cgatatgtaa tccacgctgt cgtaggctat gtcgaggcag gtatcctccc
     1561 acttgccgaa ggttttcaca tggtcgttgg agctgccgca aattacggtt tcgattgagt
     1621 catccgttag tttcattgcc tttgatgcct gatctgccaa aaaagcatat tccttcgctt
     1681 ccttgtggcc gagctgccaa ccgccgtcca tctcgttccc gaggcaccac agctttatac
     1741 cgtaagggct ttcggcaccg tttttacgtc gcatatcgct gtaatatgag ccggacgggt
     1801 gattgcagta ttcaacaaga tttcttgcgt cgtcgactcc gcgcagaccg aggtttacgg
     1861 ccatcataca agctgtgtcc gcctttttgc accacttcat aaactcatcc gtgccgaaga
     1921 gattgggctc cgtaacgccc caggcgagct caagcctttt cggcctttcg cttatgggac
     1981 ccacaccgtc ctcccacctg tatccggaaa caaagtttcc gccggggtag cgcactatcg
     2041 gtacacgaag ctttttaacc tcgtcgataa cgtcttttct gaacccgtct tcgtcggcac
     2101 aggggtgatg tggctcatat attccgccgt aaacggctct gccgaggtgt tctatgaacg
     2161 aaccgaagat tctgtcgtca accttgccta ctttaaattc gccggcaagt gataattcag
     2221 cttttttcat attgtatctc ctgaaaaatt atttcttttt ggaattctcc gttgtagtct
     2281 cgttatttga ggtgtccgcc ttttcatata tgaatgtgcg cattgccgaa acgtttttat
     2341 ccatatctat cgagagcacg ttgccggctg attttttaac gctcttccag cttccgtcaa
     2401 t
//
LOCUS       DBMDVO010000150         2393 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21028_length_2393_cov_1.102094, whole genome shotgun sequence.
ACCESSION   DBMDVO010000150 DBMDVO010000000
VERSION     DBMDVO010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21028_length_2393_cov_1.102094"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    559..568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            596..>2393
                     /locus_tag="ACZPE2_03020"
     CDS             596..>2393
                     /locus_tag="ACZPE2_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318852.1"
                     /translation="MQYSDKKQRTRVSKILSRVVAMVLCVIALTFAERIYAVTAETDN
                     RKLLNGSFEEGQTFTGNYLQPDQSAVPSWNTTAFQGKIELFRKNTGTYINGVILEPKD
                     GTYAAELNADEESTLYQNVKTTPSSVYKWGLDHGGRNRTDTMALIIGPKQSVNPSKPS
                     KEGRDQFMQMVDWLIAQGKTSVKTTAGLGEQLTVYSKKFAANGTFADNAGNNAFSLTP
                     SSIYTEEWHIWIMASSRATSGTNPWNSYGSNAEDPAGSGNGSGNTGVDLSKYYFYTVP
                     AGQTDTLFGFVSVGYVGSITTADKAKTYGNFLDNINFEIFHPLSGSTSTHGSAVVGGS
                     DGTSSGTGATAGHEVTIDNKLSTFVTDGESLKIQAIVKKADVDDGCEFVGVYQTNLEE
                     NGNSKTEFLKLAGNVIEDTGSLTEEEKAGKWLKTTNADGDIIYTYYLGNITSATDLHF
                     VFIKSPTVTYDPNGGKEYKVERTYNTDEAANVYSFKPVSGHNEAQANTGTIFIDPYVS
                     HAAEGQNDGWKFMGWKLTGDVVENVPEDKQVNAEQLGTLILPAKHTIACDYHFDGVNV
                     DTKAQYFKIYDGSKTLTKIIDTNSSDEDIGVTW"
BASE COUNT          753 a          468 c          575 g          587 t
ORIGIN      
        1 gcaaaatcca ctataccttc ggcgaagacg cttacgatgt tgttcgatat atctatgccg
       61 acagaaaggg cgatttcttc ttcgccaccg caggagaatg gattatgcac aataccggca
      121 gaaagcacat accgagatat atggttgtat ctttgtaacc gcataaaatg aaacagcccc
      181 gaagagacag aaaaaaatct gaaactcatc agggctgttt gtgttttgtt tatggtatga
      241 cagaaaattg taatattttg cgaaaaaata aaaaatgtta ataaaatctc ttgtatttgc
      301 gatgtgcatg tagtacaaca aaataaatac tacttgcgag gtgctgaaaa atgtctttaa
      361 cggtcagtta caacaagctt tggaagttgc ttgtcgataa aaaaatgagt aaggctgaaa
      421 cgcaaagcca tgatatctaa acgatttttc gcatgaccga ccggctgcaa gtttgcacac
      481 ggaaatgtgt gaattttgca gtctttttat gaaaaatgtt aaaatatcgg cttccggttg
      541 gcgtgcggac aatccattnn nnnnnnnncg gagtatcaaa aaggagtggt tacaaatgca
      601 atattctgat aaaaagcaga gaacaagagt atctaaaata ctgagcagag ttgttgcaat
      661 ggtattatgc gttatagcct taacctttgc ggaacgtata tatgcagtca ccgccgaaac
      721 tgacaataga aagctgctta acggcagctt tgaggaaggg cagactttta caggcaacta
      781 tttacagcct gatcagagtg ctgttccctc atggaataca acggcttttc agggtaaaat
      841 tgagttattt agaaaaaata caggcactta tataaatggt gtaatattag aacctaaaga
      901 cggaacttat gctgcagaac ttaatgcgga cgaggaaagt accctttacc agaatgtaaa
      961 aacaactccg tcaagtgtgt ataagtgggg actggatcac ggcggccgaa acagaacgga
     1021 taccatggca ctgattatcg ggcctaaaca gagtgttaat ccgtctaagc ctagcaagga
     1081 agggcgcgac cagtttatgc agatggttga ctggctcatt gcgcagggta agacctctgt
     1141 taaaaccaca gcgggcttag gcgaacagct tacggtttac agtaaaaagt ttgccgcaaa
     1201 cggtactttt gcagataacg cgggaaacaa cgcattcagc ctaacgccca gttcaatcta
     1261 taccgaagaa tggcatatat ggattatggc gtcctcaagg gcgacaagcg gcacaaaccc
     1321 ttggaactct tacggctcta acgccgagga cccggcgggt tcaggcaacg gctcgggcaa
     1381 tacaggggtg gatttaagca agtactattt ctataccgtt ccggcggggc agaccgatac
     1441 cctgttcggc tttgtttcgg tcggatatgt cggttctatt acaactgcgg acaaagcaaa
     1501 gacctacggc aactttcttg ataacattaa ctttgagatt ttccaccctc tgtcaggaag
     1561 cacctctacc cacggtagtg cggttgtcgg cggaagtgac ggaacaagca gtggtacggg
     1621 cgctacggcg ggccatgagg taacgattga caataagctt tcaacctttg ttaccgacgg
     1681 tgagtccctt aaaattcaag ccattgtgaa gaaggcagat gttgatgacg gctgtgaatt
     1741 tgtaggtgtg tatcaaacaa atcttgaaga aaacggaaat tccaaaactg aatttttgaa
     1801 attggcggga aatgtaattg aagacacggg cagcttgacc gaagaggaga aagcgggtaa
     1861 atggctaaaa accacgaatg cagacggcga tattatttat acctattatc ttggtaatat
     1921 cacttcggca accgacctgc attttgtatt tatcaagagc cctaccgtaa cctacgaccc
     1981 caacggcggt aaagaatata aggttgaaag gacgtataat acagatgaag cggcgaatgt
     2041 atacagcttc aagcctgtgt cgggtcataa cgaggcgcag gcaaacacag gcacaatatt
     2101 tattgacccg tatgtttcgc atgcggcgga gggacaaaac gacggttgga aatttatggg
     2161 ttggaagctt acgggcgacg ttgtagaaaa tgttccggaa gataagcagg taaatgcaga
     2221 acaattagga actctgatac ttcccgccaa acataccata gcctgcgatt accactttga
     2281 cggagtaaac gtggacacaa aggcacagta ttttaagatt tatgatggca gtaaaacctt
     2341 gacaaagatt attgatacga actcttccga tgaggatata ggtgtaacct gga
//
LOCUS       DBMDVO010000151         2374 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21249_length_2374_cov_1.605807, whole genome shotgun sequence.
ACCESSION   DBMDVO010000151 DBMDVO010000000
VERSION     DBMDVO010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21249_length_2374_cov_1.605807"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="ACZPE2_03025"
     CDS             complement(<1..445)
                     /locus_tag="ACZPE2_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR1318853.1"
                     /translation="MGGYVLAVLKDVKRLVVKVGTSTLVYDNGKTNIRRMHKLISVLS
                     DIENSGIKVTLVSSGAIGVGTGKLGLPERPKDTPGRQAASTVGQCELMYMYDKMFSEY
                     GHKIGQLLFTKSDVESPERRKNLINTFDKLFSYGAIPVVNENDAIG"
     gene            731..1420
                     /locus_tag="ACZPE2_03030"
     CDS             731..1420
                     /locus_tag="ACZPE2_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1318854.1"
                     /translation="MYNVLVCDDDKAILDSLEIYLNLEGYKVFKAENGRQALKTAEEN
                     DLQCIILDIMMPDLDGLNATLKLRESKNIPIILLSAKSEDTDKITGLSFGADDYVTKP
                     FNPLELMARVKSQIRRYVSLGSMEKKDSLIVTGGLALDLDSKEITLDGEKVKLTATEY
                     GILEYLMKNIGRVLSTNQIYEAVWKEPSFQTEKTVTVHIRRIREKIEINPKEPKYLKV
                     VWGIGYKIEKL"
     gene            1395..>2374
                     /locus_tag="ACZPE2_03035"
     CDS             1395..>2374
                     /locus_tag="ACZPE2_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318855.1"
                     /translation="MATKSKSFKTSFGLKLVAFFIAALLAFSSAICAVEAINDLFRYG
                     SEILTKPAKSLDMTETSLFASKLNSDVQSIYAGSGLMTWDAYKSTYYSTFDEELSAAR
                     TELERFKKECMSEAVAAYRDKYAASEYSSSQKSDDVTGAGASAYTTNVYGNVSAYSRS
                     DNVVSPNYDKDYYDGCRESYSLKSGDSGNVTFTTSTTDEELVSLVSNAYSQVMSAARR
                     EYETTVAMESRSIKNIKNLKYYIYNPSTGEIITNLETETSEEKANEIKKSGDYSAFTS
                     GSRWYMVYTADGGLVAASPQITRDTLYTVQSGDKYYFSLKSDYYYKRTLS"
BASE COUNT          687 a          509 c          569 g          609 t
ORIGIN      
        1 cgcctattgc gtcgttttcg tttactacgg gaattgcacc gtatgagaag agtttatcaa
       61 aggtatttat aagatttttg cgcctttcgg gggactccac gtcgcttttt gtgaacagga
      121 gttggcctat tttatggccg tattcgctga acatcttgtc atacatatac ataagctcgc
      181 attgacctac ggtggacgcc gcctgcctgc ctggggtgtc cttcggacgc tccgggagcc
      241 cgagctttcc cgtgccgaca cctatcgcgc cggaggagac gagagttacc ttgatgcccg
      301 aattttcaat atccgagagt accgagataa gcttatgcat tcttcttata tttgtttttc
      361 cgttgtcgta gacaagcgtg gaggttccca ctttgacaac gagccttttt acatccttta
      421 ataccgccaa gacatatcct cccacaagcc ccgccttaca gggcgcgaaa taataatatt
      481 tatacactat aaattatatc atacgaaaag ggatttaaca atataaaata tttttcgcga
      541 caccttttta aatttaaccg aattgtaatt ttttgtatat gcttttgtaa tttcgatgtt
      601 gtataaactt actgtgtagg gagtcgtctc gaaaaacgga gatacaaaat atttgtattc
      661 cgttttgtgt gataatctgt taaaataaaa tcagccgagc aggcgaaaaa tacggaagag
      721 gggcaaagtt atgtataacg ttcttgtgtg tgacgacgac aaagcgatac tcgactcact
      781 cgaaatatat ttaaatcttg aggggtataa ggtttttaag gcggaaaacg gaaggcaggc
      841 attgaaaaca gcggaggaaa acgatctgca atgtataatt ctcgatatta tgatgcctga
      901 cctcgacgga ctcaacgcaa cgctgaagct ccgtgagagc aaaaacatac cgataatcct
      961 actctctgcc aagagtgagg atacggacaa gataacgggt ctttcgtttg gagcggatga
     1021 ctatgtgaca aagccgttta atccgcttga gcttatggca cgggtaaaat cgcagattag
     1081 gcgttatgtt tcgctcggca gtatggagaa aaaggacagc cttatagtta cgggagggct
     1141 cgctcttgac ctcgattcca aagagattac gctcgacggc gaaaaagtca agcttaccgc
     1201 tacggaatac ggcatactcg aatatcttat gaaaaatata ggcagagttc tctctacgaa
     1261 tcagatatac gaagcggtgt ggaaggagcc gtcgtttcag acggaaaaaa ccgtgaccgt
     1321 gcacatacgc agaataagag aaaaaataga gataaatccc aaagagccga aatatttaaa
     1381 ggtggtgtgg ggcattggct acaaaatcga aaagctttaa aacttcgttc ggattaaaac
     1441 tcgttgcatt ttttattgcg gcgttgcttg cgttttcctc ggcaatttgt gcggttgagg
     1501 ctataaacga cctgttcaga tacggctcgg aaatccttac taagccggcg aaaagtcttg
     1561 atatgaccga aacgtcgctg tttgcatcga agctgaactc cgatgtgcag tcgatatatg
     1621 ccggcagcgg ccttatgacg tgggacgcat ataaaagcac gtattacagc actttcgacg
     1681 aggagctgag cgcggctcgc acggagctgg agcgcttcaa aaaggaatgt atgtcagagg
     1741 ctgtggcggc atatcgtgat aaatatgccg cttccgagta ttcctcttct caaaagagtg
     1801 acgatgttac gggggccggt gcttccgcct atacgacaaa tgtctacgga aacgtctctg
     1861 cctattcccg ctccgacaac gtagtttcac cgaattacga caaggactac tatgacggct
     1921 gccgtgagag ctattcgctt aagagcggag attcggggaa tgtcaccttt acaacctcca
     1981 ccacggacga ggaactggta tcgctcgtga gcaatgcata ttctcaggtt atgtctgcgg
     2041 cacgccgtga gtatgaaaca acggtcgcca tggagagccg cagcatcaaa aatataaaga
     2101 accttaaata ctatatatat aatccgtcta ccggcgaaat aatcacaaat cttgagacgg
     2161 aaacgagcga ggagaaagcg aacgaaatta aaaaatcggg agattattcg gcgtttacaa
     2221 gcggaagcag atggtatatg gtctatactg ccgacggagg acttgtcgcg gcaagtccgc
     2281 aaataacccg cgatacgctc tataccgtac agagcggcga taaatactat tttagcctta
     2341 agtcggatta ctactacaaa cgcacgctgt caaa
//
LOCUS       DBMDVO010000152         2372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21286_length_2372_cov_1.184060, whole genome shotgun sequence.
ACCESSION   DBMDVO010000152 DBMDVO010000000
VERSION     DBMDVO010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21286_length_2372_cov_1.184060"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="ACZPE2_03040"
     CDS             <1..432
                     /locus_tag="ACZPE2_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318856.1"
                     /translation="VGVAVGTGVGVGLGVGVAVGVAVGVAVGLGVGVELGVGVGVAVG
                     VAVGVAVGTAVGVAVGSAVGVAVGSAVGVAVGSAVGVAVGSTVGVTVATGMIPIWESN
                     SSFFSGNTNHPSKNTAIIKISAMMTPIRQPPPPRFFLLRCS"
     gene            890..1153
                     /locus_tag="ACZPE2_03045"
     CDS             890..1153
                     /locus_tag="ACZPE2_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318857.1"
                     /translation="MMISPYALLKASKPKTSADFVKPYDLYCAAQHAQSEHVSIDRES
                     TPPQPFDVPPAEMPAGKTHYIRNIGMRHNQALRYAALSQTPID"
     gene            complement(1135..1608)
                     /locus_tag="ACZPE2_03050"
     CDS             complement(1135..1608)
                     /locus_tag="ACZPE2_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318858.1"
                     /translation="MGKSYVLKEKNERICGFVMQMGSNLSYRITHFVKKPLMLTAVYS
                     EGESICTEVTDSDEHRQKAGSGELLGAYAVFDGELLMDTGEDARNAYCRRKSNLTRKA
                     QFSSCNSANTEIGARALEKDVLTDSIRKERRWPPPPCISYARFLNGKWIDQSMGV"
     gene            1923..>2372
                     /locus_tag="ACZPE2_03055"
     CDS             1923..>2372
                     /locus_tag="ACZPE2_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1318859.1"
                     /translation="MRNANADGTPKILYTGQSKITMNMRSQADKESPSLGVLKAGDTV
                     QIFGFDQEWLFCWQPDVGIYYVGRHNVDNITPIADGIASYGVVPNRFVAVTAVDTALY
                     AEPDSESEQLVTLAANSRFSIWLIQDGWAVVPYKRVIGYMYVGDIKEL"
BASE COUNT          528 a          598 c          551 g          695 t
ORIGIN      
        1 gtgggtgtcg cagtcggcac aggcgtaggt gtcggactcg gcgtcggcgt tgcagtcggc
       61 gttgccgtgg gcgtcgcggt cggcttgggc gtaggtgtcg agctcggtgt cggcgtaggt
      121 gttgcagtcg gcgttgccgt gggcgtcgcg gtcggtacag ctgtaggtgt tgccgtaggt
      181 tctgccgtag gcgttgcggt cggctctgct gtcggcgtcg ccgtaggttc tgccgtgggt
      241 gttgcagtcg gctcaaccgt cggcgtcaca gttgcaacag gcatgattcc aatctgggag
      301 agcaattcat ccttcttctc cggaaataca aaccatccca gcaagaatac ggcaataatc
      361 aaaatcagcg caatgatgac gccaatcagg cagccgccgc ctccgcgatt ctttctgctg
      421 cgatgttcat aatcatcatc gaaatcatca tcgtcatcat cttcattcca aacctgctgc
      481 tgaacgtgat ttggacgcgg catagtcggt ctatttacct cggaagatcg agcagattct
      541 ttaacgccga attccgcatc tgtatgtgca tgtaattgcg gctgttcgaa ttcttctttt
      601 atacttcctt cgtcggcaag ttcttcctgt tgaagcctat cgaactcttc aaaagccttc
      661 ttctgtgctt cagcacgtct gcgccttttc ggtgcctgcg taccgtttat ctgttcatcc
      721 aaaacttaca cctcatttca ggcaatcttt aatgggtaca ttatacatga aattctgata
      781 tttctcaatc ccaatttaga gaaaagctgg aaagccagct attttgcgtg acatttcgtc
      841 ttttcgttca tagaatgcga acagacagcg tcgtccgaaa ggagtcttca tgatgatttc
      901 accttatgcg cttttaaaag ccagcaagcc taaaacgtca gccgactttg tcaaacctta
      961 cgatttatat tgtgccgcgc agcacgcgca aagcgaacat gtatccattg accgtgaatc
     1021 cacgcctccg cagcctttcg acgtaccccc tgctgagatg cctgccggca aaacgcatta
     1081 catacgtaat atcggcatgc gtcataatca ggcacttcga tacgcagctc tttctcaaac
     1141 gcccatagac tgatcaatcc atttgccgtt caaaaaacgt gcgtaggata tacatggcgg
     1201 cggaggccat cgcctttctt tcctgataga atctgtgaga acgtcttttt ctaaagctct
     1261 tgctcctatt tctgtgttcg cagagttgca tgagctgaat tgcgctttgc gagtaagatt
     1321 gctttttctg cgacaatatg cgtttcgagc atcttctcct gtatccataa gcagttctcc
     1381 gtcaaatacc gcataagcac ccaataattc gcctgaacct gctttctgcc ggtgttcatc
     1441 actatctgta acctctgtgc agatactttc accttccgaa tatactgctg tcagcattaa
     1501 tggtttcttc acgaaatgcg taatgcgata actcaggtta ctgcccatct gcatgacaaa
     1561 tccgcagatt ctttcgtttt tttcttttaa aacatagctt ttccccatac cggaacacct
     1621 ccctgcaaaa ctatgctgac tcggcacttg ccttgcttcg attttcgcag tataataaaa
     1681 tgaatgggcg gagtctgagg ccgcttctgg cgaggctcgt tatggcctga tatctttttt
     1741 acattgccca tacttacgta attctttatg aagacgcaaa taattcttat tcagctcaaa
     1801 tgattgatgg aggctcacct gtgcgaaata cgctgattac cctgtttatc tgtctgttgg
     1861 caacttttct tcccgtttgt gcatctgctg ataccggcac cgcaatctgc tttgaggagg
     1921 ccatgcgcaa cgcaaatgcc gatggcacac cgaagatttt atataccggt caatctaaaa
     1981 tcactatgaa tatgcgcagt caggcagata aggagagtcc ttctcttggc gttcttaaag
     2041 cgggcgatac cgttcaaata ttcggttttg accaagagtg gctcttctgc tggcagcctg
     2101 atgtcggaat ttactatgtc gggcggcata atgtcgataa tatcacgccg attgctgacg
     2161 gtatcgcttc ttatggtgtt gttcccaacc gttttgttgc cgtaaccgcg gtcgatactg
     2221 cattgtatgc cgaaccggac tcggaatctg aacagctcgt tacgcttgca gccaactccc
     2281 gcttcagtat ttggctgatt caggatggtt gggcggttgt accgtataag cgtgtgattg
     2341 gctacatgta tgtaggcgat atcaaggaac tg
//
LOCUS       DBMDVO010000153         2368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21345_length_2368_cov_1.556683, whole genome shotgun sequence.
ACCESSION   DBMDVO010000153 DBMDVO010000000
VERSION     DBMDVO010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21345_length_2368_cov_1.556683"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="ACZPE2_03060"
     CDS             <1..434
                     /locus_tag="ACZPE2_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1318860.1"
                     /translation="RPYNRKNNVTSYNPFREMDSFFDDPFRFFDGKALAEFNTDIIES
                     GNNYILEADLPGFEKGDIKLDVKDDILTIKAERHTETEKKDKDSRSICCERSYGSYSR
                     QFDVSGIDTDKISAKYENGVLKLTMPKKEAPKETSKHLEIE"
     gene            complement(495..923)
                     /locus_tag="ACZPE2_03065"
     CDS             complement(495..923)
                     /locus_tag="ACZPE2_03065"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1318861.1"
                     /translation="MIKSTWFLGGTACPDGDEIRKKVFVNEQKYPISVEFDDHDKTCD
                     HLLLTVDGTPAATARVFMTDEQTAKIGRLAVLKEYRSQHLGAELIKELKKRSKALGAK
                     NIYVSAQVYAVPFYEKYGFRAYGNEYLDIHIPHMDMDCPL"
     gene            complement(965..>2368)
                     /locus_tag="ACZPE2_03070"
     CDS             complement(965..>2368)
                     /locus_tag="ACZPE2_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014926.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit-related
                     protein"
                     /protein_id="HIR1318862.1"
                     /translation="YSSDKLMGGCIWEWCDHAVYHGKDDKKYKYEYTYGGDHGEEMHD
                     KNFCVDGLVFPDRTLHTGALEMKHAYRPVRSVVSGDNTLLLTNTYRFLSTDVLTVKWE
                     LCFDCEKVKDGVIDKVIAPSATAEVTLPLGRIPSDRLVTLNIFYEDKNGAEISREQHV
                     LCDAPAAIETGDKKVLLTESDSGYSAEFDNGKIEFSRSTGEILSYTADSTELISKTPL
                     SGISGFLPNIYRAPSDNEEVNPIPKWNREKLAMVKAVYKGMRAESEEKEVKITAYYDM
                     TDGKRLYYKSFIEYTVFSDGTVKVRSSLARKRYGWKELPRFGLTAELPKEFSNVKYLG
                     RGPYENLCDFNGQSPIGLYKTTVKEMHVDYIKPQDNGNHGAVRFAEFTDGNGKGLAFL
                     GAPKFSFSAHDYTQKILCAARHREDVIHEDTTFVSIDGFVRGTGTASCGQDTLMKYRF
                     VLDDTIEFRFAMKPIAR"
BASE COUNT          560 a          569 c          546 g          693 t
ORIGIN      
        1 taagacccta taaccgtaaa aacaatgtta catcctataa tccgttccgt gaaatggaca
       61 gcttctttga tgacccgttc agattcttcg acggtaaggc gcttgccgaa ttcaataccg
      121 acattattga gagcggcaac aattatatcc ttgaagcaga tcttcccgga tttgaaaagg
      181 gcgacattaa gcttgatgtc aaggacgata tcctcaccat caaggcagaa cgtcacaccg
      241 aaaccgagaa aaaggacaag gacagcagga gtatctgctg cgagcgttcc tacggctcat
      301 attcaagaca gtttgacgtt tccggtattg ataccgataa gatttcggct aaatacgaaa
      361 acggtgtcct gaagctcaca atgcctaaga aggaagctcc gaaagagact tcaaagcatc
      421 ttgagattga ataatcaaaa gataacgtga cccggagagc gacagctccc cgggtctttt
      481 tttgctctta atgtttataa cgggcaatcc atatccatat gcggaatgtg tatatcgaga
      541 tattcattcc cgtatgccct gaatccgtat ttttcgtaaa acggaacggc atatacctgt
      601 gcgctgacgt atatattttt cgcgccgaga gccttagacc tctttttaag ctcttttata
      661 agctctgcgc cgagatgctg actgcggtat tcctttaata ccgcaagcct gcctatcttt
      721 gccgtctgct cgtccgtcat aaaaacgcgc gccgttgcgg caggtgtgcc gtcaaccgtt
      781 aaaagcagat gatcgcatgt tttgtcgtgg tcgtcgaatt cgacggaaat agggtatttc
      841 tgctcattca caaatacctt ttttctgatt tcatcgccgt cggggcaggc tgtgccgcct
      901 aaaaaccaag tgctttttat catagcttaa aaataacctt aacctttccg gctctttcgg
      961 gagcttatct tgctatcggc ttcatggcaa agcggaattc gattgtgtcg tctaatacaa
     1021 atctgtattt cataagcgtg tcctgaccgc agctcgccgt gcctgttccg cgaacaaaac
     1081 cgtcaattga aacaaacgtg gtgtcctcgt gtattacgtc ctcgcggtga cgtgccgcgc
     1141 aaaggatttt ttgtgtgtaa tcatgagccg agaagctgaa tttcggagca ccgaggaacg
     1201 ctaagccttt tccgttaccg tcggtaaatt ccgcaaatct tacggctccg tggttgccgt
     1261 tatcctgagg cttgatatag tcaacgtgca tttctttaac ggttgtttta tataagccta
     1321 tcggggattg accgttaaaa tcacagaggt tttcatatgg ccctctgccg aggtatttca
     1381 cgtttgaaaa ttccttcggg agttccgctg taaggccgaa tctcggcaac tctttccagc
     1441 cgtatctctt cctcgcgaga gaggaacgaa ctttaaccgt gccgtcggaa aatacggtgt
     1501 actctataaa gctcttgtag tagagccttt taccgtcagt catatcgtaa taagcggtta
     1561 tttttacctc tttttcctcg ctctcggctc tcattccctt gtagaccgct ttgaccatag
     1621 ctagcttttc gcgattccac ttcggtatgg gatttacctc ttcgttgtct gacggagcac
     1681 ggtaaatatt cggtaagaag cctgagatgc cgctcaacgg agttttgctg ataagttctg
     1741 tgctgtctgc cgtatatgag agaatttctc cggtggaacg tgagaattcg atttttccgt
     1801 tatcaaattc cgccgagtaa ccggagtcgc tctccgtgag aagaactttt ttatcgcctg
     1861 tttcgattgc ggcaggagcg tcgcagagta cgtgctgctc tcttgatatt tcggctccgt
     1921 ttttgtcttc gtaaaatatg ttgagcgtta caaggcggtc gctcggtatt cggccgagcg
     1981 gcagcgttac ttccgcggta gcagacgggg ctattacctt gtcgataacg ccgtccttta
     2041 ccttttcgca gtcgaaacaa agctcccatt ttacggtaag aacatctgtg cttaaaaatc
     2101 tgtatgtgtt tgtaagaaga agtgtgttgt cgcccgatac cactgacctt acgggtctgt
     2161 atgcgtgctt catttcaagc gcgccggtat ggagcgtacg gtcggggaat acaagaccgt
     2221 ctacgcagaa gtttttgtcg tgcatttcct cgccgtggtc gccgccgtag gtgtattcgt
     2281 acttgtactt tttatcatcc ttgccatggt ataccgcatg gtcgcaccat tcccatatac
     2341 agccgcccat aagcttgtcg cttgaata
//
LOCUS       DBMDVO010000154         2343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21689_length_2343_cov_1.242194, whole genome shotgun sequence.
ACCESSION   DBMDVO010000154 DBMDVO010000000
VERSION     DBMDVO010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_2343_cov_1.242194"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..>1552
                     /locus_tag="ACZPE2_03075"
     CDS             20..>1552
                     /locus_tag="ACZPE2_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318863.1"
                     /translation="MRKTKFQKVLAISLIAIMLCSCATKEKDNFDSASNPFDASAQEN
                     MPPDSDNAKELYERYQTGGASVDFSKMDGKDTLEYNGSPIEITFSFDTSENEYDMTEG
                     FVAFICGVPQMISADGGETSEMAKVTYEKKTTTETTLTVNPRITEDLKGQDELTLMIA
                     SLHSVDYVPQGKYAGYGFNNIHSSSPLCYYKLKINAQPEIINLDGKIEFENVNASDKS
                     LENYNLKNAGEIGDDCMMKMYSVENQSPRLVLDGSKQSLDLVFNGMNADRFYVYLYVN
                     MQRVKFDGSDYAVVNSKSGFVPKLTVTLDNLSSRDVIYAIAVSPDSESKFPAYTKTMS
                     SLCFAPDDELLSAGSNSAGEQTEAVTTDISKPGDAPDNTSTAVSDNPIYKFKPLGYLD
                     DDQNLLLMYIDNDFKVYGEDNGTYFTVYDVTAKEFISGKFLPYQQTFTVTYGQGCFTV
                     IEGKASEDNRSLAECMAVTYNEKFEEIQRAHNMPMYSGGNQSIYSAYYCKDIDKYLCA
                     YKE"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1563..2194
                     /locus_tag="ACZPE2_03080"
     CDS             <1563..2194
                     /locus_tag="ACZPE2_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318864.1"
                     /translation="LTVLLNTDGSVYKVINTDSETSVYSFSVDDDKIILGRRYDDYRT
                     KIQVMDFDGNIIDEKTMSAEYANLDVNRFGKYIAFLSLKRYGEDENPGSDSFAVLYDT
                     ETDKLMEFTPETADEAGCMGISPDGKIAVTMDYEEVDDDGIVTGQKQTLRYYDIESGK
                     VIKTVNADDKPALNNFVVYDDGVIQTVPGYNKEKNERTETVVFFDRFDD"
     gene            2204..>2343
                     /locus_tag="ACZPE2_03085"
     CDS             2204..>2343
                     /locus_tag="ACZPE2_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1318865.1"
                     /translation="MKLEINQLTKSYSKGKKALDSFTLTLEKGVYGILGPNGAGKSTL
                     IN"
BASE COUNT          763 a          424 c          522 g          624 t
ORIGIN      
        1 actatatttt tcggaggtaa tcaggaaaac taaattccaa aaagtacttg ccatttcact
       61 tatcgcaata atgctttgtt cctgtgcaac aaaagaaaaa gataattttg acagtgcttc
      121 aaacccattt gatgcaagcg cacaggagaa tatgccgccg gattccgata atgcaaaaga
      181 gctttatgaa aggtatcaga caggcggtgc aagtgtggat ttttccaaaa tggacggcaa
      241 agatacttta gaatataacg gcagtccgat tgaaataacc ttttcatttg atacaagcga
      301 aaatgagtat gatatgactg agggatttgt ggcgtttatt tgcggcgtgc cgcagatgat
      361 ttcggctgac ggcggagaaa cttccgaaat ggctaaggtt acatacgaaa agaaaacaac
      421 caccgaaacc acattaaccg tcaatccgag aatcaccgag gatttgaagg gacaggacga
      481 gcttacgctt atgatagcaa gtttgcacag cgttgattat gtgcctcagg gaaaatatgc
      541 cggatatgga tttaacaaca ttcattcaag cagtcctctt tgctattata agctgaagat
      601 aaatgctcag cctgagatta taaaccttga cggcaagatc gaatttgaga atgtaaatgc
      661 ttccgataag tcacttgaaa actataatct taaaaatgca ggggaaatcg gtgacgactg
      721 catgatgaaa atgtacagtg tagaaaacca atctccgcgc cttgtccttg acggcagtaa
      781 gcagagctta gatctggttt ttaacggtat gaacgccgac aggttctatg tatatttgta
      841 tgtaaatatg cagcgtgtaa aatttgacgg ttcggattat gcggttgtta attcaaaatc
      901 gggttttgtt ccgaaattaa ccgtgacact tgataatctc agttcaagag atgtgatata
      961 tgcaatagct gtttcgcctg attctgaaag taaatttccg gcatacacaa agactatgag
     1021 cagtttgtgt tttgctccgg atgatgagct cctttcagca ggaagcaaca gtgcaggtga
     1081 gcagaccgaa gcagtgacta ccgatatatc aaaacctggc gacgctcccg ataatacctc
     1141 aacagcggtt tcggacaacc cgatttataa atttaagccc ctcggatatc tggacgatga
     1201 ccaaaacctt ctgcttatgt atattgataa tgactttaaa gtttacggcg aagacaacgg
     1261 tacatatttt actgtttatg atgtaacggc aaaagaattt ataagcggaa aatttttgcc
     1321 ttatcagcag actttcactg ttacttacgg acagggctgt tttacggtaa ttgaaggtaa
     1381 agcgtctgaa gacaatcgca gccttgccga gtgtatggca gttacttata atgaaaaatt
     1441 cgaagaaatt cagcgggcgc ataatatgcc gatgtattca ggcggtaatc agtctatata
     1501 cagtgcttat tactgcaagg atatcgacaa atatctctgt gcttataaag aannnnnnnn
     1561 nnatctgacg gtactgctta atacagacgg ctcggtttac aaagtgatta acacagacag
     1621 cgaaacaagc gtatacagct tttctgttga cgatgataaa atcatattgg gacgaagata
     1681 tgacgactac cgcacaaaga ttcaggttat ggattttgac ggaaatataa tcgatgaaaa
     1741 aactatgtca gcggaatatg caaatcttga tgtaaaccgt ttcggaaaat atatcgcttt
     1801 tctttcgtta aaaagatacg gtgaagatga aaatccgggt tcggacagct ttgcggttct
     1861 ttatgataca gaaactgaca aacttatgga atttacgcct gaaaccgctg acgaggcagg
     1921 atgtatgggt atttcgcctg acggcaaaat tgcggtaact atggattatg aggaagttga
     1981 tgacgatggt attgtaacag gtcagaaaca aactctgaga tattatgata tcgaaagcgg
     2041 taaagtaatc aaaaccgtaa acgccgatga caagccggct ttgaataatt ttgtagtata
     2101 tgatgatggc gttattcaga cggtaccggg ctacaataag gaaaagaatg aaagaacgga
     2161 aacagttgta ttctttgacc gatttgacga ttaaggtgga aaaatgaaac tcgaaatcaa
     2221 tcaactcacg aaatcctata gcaaaggcaa aaaagccctc gacagcttta ccctcaccct
     2281 tgaaaaaggc gtgtacggca tcttaggtcc caacggtgcg ggcaaatcca cgttgataaa
     2341 ctg
//
LOCUS       DBMDVO010000155         2341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21719_length_2341_cov_1.320982, whole genome shotgun sequence.
ACCESSION   DBMDVO010000155 DBMDVO010000000
VERSION     DBMDVO010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21719_length_2341_cov_1.320982"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="ACZPE2_03090"
     CDS             <1..276
                     /locus_tag="ACZPE2_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HIR1318866.1"
                     /translation="LYIAVLTDPTTGGVTASFAMEADIILAEPGATVCFAGARVIEQT
                     TKKALPEGFQKSEFVLEHGFIDSIIPRSNQKKCLTELLKMHDGRAMM"
     gene            273..1064
                     /locus_tag="ACZPE2_03095"
     CDS             273..1064
                     /locus_tag="ACZPE2_03095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR1318867.1"
                     /translation="MSESAFERVKLARDSKRPTGLDYIKNIFRGFIEFHGDRRYADDP
                     AIVGGIAKLDSTPVTVIAIEKGHTAKERTYRNFGMPQPEGYRKALRLMKQAEKFGRPI
                     VCFVDTSGAYCGISAEERGQGQAIAENLLEMSTLCVPIISILIGEGGSGGALALATAD
                     RVWMLQNAVYSVISPEGCASILWKDSAKAETAAASLRLTAEDAKSLGVIERILSENDI
                     GKKEFYDRIRTLLTEEIKELSGDIDLMQNRYDRFRNLGVSAVIKE"
     gene            1067..>2341
                     /locus_tag="ACZPE2_03100"
     CDS             1067..>2341
                     /locus_tag="ACZPE2_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1318868.1"
                     /translation="MSIYKQYCNEIVDENGRLQKLTLEYPDNFNFGYDVVDKIADETP
                     EKRALVWCNVEGEEHIFSFADIKKYSNQMANVFRSAGIGHGDRVILILKRHYEYWFAA
                     VALHKLGAVMIPATHMLTVSDFVYRIKSSKAKAIVCTTQNEVPEKITAALKEADMTAK
                     LWCVQKDADGFENLTEAMREAPEKFERVQTLATDPMMLYFTSGTTGYPKGVIHEHSYP
                     LAHIVTAKYWHQAEDNGLHFTVAETGWAKASWGKIYGQWLVGSAVMVYDFDNFEPKQL
                     TAVINRYGVTSFCAPPTVYRYLVRKGIPDMPPLKHATTAGEMLAPEVFRKFTERTGLQ
                     LCEGYGQTETTLLMANFKGYEAVEGSMGTPSPFYNIELRGKNGKPVDTYEIGEVVIVP
                     EKAGKQPGVFAAYLDNEEQYSYVWRDGVYHTGD"
BASE COUNT          646 a          493 c          623 g          579 t
ORIGIN      
        1 ttgtatattg ctgttcttac tgatccgaca acgggtggtg tgacggcgag cttcgctatg
       61 gaagcagata ttattcttgc agaacccggt gcaactgtct gttttgcagg tgcgagggtg
      121 attgagcaga caacaaagaa agctctcccg gaaggattcc aaaagtctga atttgtactg
      181 gaacacggat tcattgacag cattattccg cgctctaacc aaaagaaatg tttgactgag
      241 ctgttaaaaa tgcacgatgg gagggcgatg atgtgagcga atcagctttc gaacgagtaa
      301 aacttgcccg tgacagcaaa agaccgacgg ggcttgatta tattaaaaac attttcaggg
      361 gctttatcga gtttcacggt gatcgccgtt atgcggatga tcccgctatc gtaggtggga
      421 ttgcgaagct ggatagcact cccgtgacgg taattgcgat tgagaaaggt catactgcaa
      481 aagagcgtac ttaccgcaat ttcggtatgc ctcagccgga aggctaccgc aaagccctgc
      541 gtcttatgaa gcaggcagag aaattcggca gaccgattgt ttgctttgtg gatacttccg
      601 gtgcgtactg tggaatcagt gctgaagaac gggggcaagg acaggcaata gcagaaaacc
      661 tgctggaaat gtctacgctt tgtgtgccga ttatctctat tttgattggc gaaggcggca
      721 gcggtggtgc tcttgcactt gcgactgcgg acagagtatg gatgctgcaa aatgccgtgt
      781 attccgttat ttcaccggag ggctgtgcaa gtattctttg gaaagactct gccaaggcag
      841 aaactgcggc agcaagcctt agattaaccg ccgaggatgc taagagcctt ggcgtaatag
      901 aacgcatcct ttccgaaaat gacatcggca aaaaagaatt ttacgaccgc atccgtaccc
      961 ttttaacgga agaaatcaag gaattgtccg gcgatattga cttgatgcag aatcgctatg
     1021 acagattccg taatcttggc gtcagtgctg ttattaagga gtaaatatga gcatatacaa
     1081 acagtattgt aatgagattg tcgatgaaaa cggcaggctg caaaaattga cacttgaata
     1141 tcctgacaac tttaacttcg gttatgatgt tgtcgataaa attgcagatg aaacaccgga
     1201 gaaacgtgcg cttgtatggt gcaatgttga aggcgaagag catatttttt catttgcaga
     1261 tataaagaaa tacagcaatc agatggcaaa tgtatttcgc agtgcgggaa tcggtcacgg
     1321 cgaccgtgtg atattgattt tgaagcggca ctatgaatat tggtttgcgg cagttgcgct
     1381 gcacaagctg ggtgcggtaa tgatcccggc gacccatatg ctgacggtca gtgactttgt
     1441 atatcgcatt aagtcctcta aagcaaaagc gattgtgtgt accacacaga atgaggtgcc
     1501 cgaaaaaatc actgcggcgc tgaaagaggc agacatgacg gcaaaactgt ggtgcgttca
     1561 gaaggatgca gacggctttg aaaatctgac ggaggctatg agagaagccc ctgaaaaatt
     1621 tgagcgtgtg cagaccttag ctaccgatcc gatgatgttg tatttcacat cgggtactac
     1681 cggttatccc aaaggcgtca ttcacgagca cagctatcct ctcgcacata tcgtcacggc
     1741 aaagtattgg catcaggcag aggataacgg actccacttc acagttgcgg aaacaggatg
     1801 ggcaaaggca tcctggggaa agatttacgg acagtggctc gtcggcagcg ccgtcatggt
     1861 gtatgacttc gataactttg aacccaaaca attaaccgct gtgatcaatc gttatggcgt
     1921 aacttctttc tgcgcgcctc ctacggttta ccgctacctt gtgcgtaaag gtattcccga
     1981 tatgccgccg ctcaaacatg caacaacggc aggtgaaatg ttggctcccg aagtattccg
     2041 taagtttaca gaacgcaccg gacttcaact ctgcgaggga tacggtcaga ccgagacaac
     2101 gttattgatg gcaaatttca aaggttacga agctgtagag ggttctatgg gtacaccctc
     2161 gcccttttac aatattgagc ttcgcggaaa gaacggaaaa cccgtcgaca cctatgaaat
     2221 aggcgaggtt gttattgttc ccgaaaaagc gggcaagcaa cccggtgtgt tcgccgcata
     2281 tctggacaat gaggaacagt atagttatgt ttggcgtgac ggtgtgtacc atacgggtga
     2341 t
//
LOCUS       DBMDVO010000156         2337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21778_length_2337_cov_1.104651, whole genome shotgun sequence.
ACCESSION   DBMDVO010000156 DBMDVO010000000
VERSION     DBMDVO010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21778_length_2337_cov_1.104651"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2337)
                     /locus_tag="ACZPE2_03105"
     CDS             complement(<1..>2337)
                     /locus_tag="ACZPE2_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HIR1318869.1"
                     /translation="DGVDGNKYGGWVLGGYGSSSDVDITAMAIQALAPYYNDDTVYTY
                     KNEISGDEVSKTVRQCVDEAFDRLGSMMNDKAGFTSWNTDNSESIAQVIVALTSVGID
                     PQKDSRFITSDGLTLLDGLLRFRDTNGGFCHVVGNGWNSMANDQATYALVAYWRFENK
                     MRALYDMRGDWSAETKAAITDAVSAIDALPLPTDANYKSAAKSALAKFRAVPENERRY
                     VNNYSALVSAIELVGGEGELDSNTPYITSIAVTKAPDKTEYYDGDSFDAAGMEVTAYY
                     SDGAQKPVTDYEIISGDSLEVGTQKVCIKSGKLKTFVTVTVKDKIPWEGLGSETSPYL
                     INTAEDLYNLSYYVNTKLMATTGVYFEMTDNIDLSDYDNWTPIGSDRNQFDGVFDGDG
                     YVIDNLTSLRGGLFGTVCQNAVIKNVGVASGEIGKENIYTSFLGGIAKWSNGADFINC
                     WNGADIYGSGYMGGIVGTVRDGGKSNITGCYNVGSLYASSGHTGGIVGHLDTTRRDTS
                     VEVTIDNCYNLGSINGIYSLGGIVGQAQDGHTIVNCYNAGKIKALWYPSGIISNVSGK
                     NSAKSVIENCYNIGEISVFPDKTFLAGISANCSAATKNCYSLYPAADSFGSGKNGASE
                     KITSPDGLLEKLGSAYIADSNAVNSGYPIFPWQEGKKAEPETPSVKIQGAASIYMTNS
                     GEQPTLTLTAKLTALDSETAVIWSIEDGADFAKLDQSDNNAVITPLSPGKITVKAETA
                     DGNYFDTAEIFIIPHINSIKIGGTVAVGETVRAIVYTF"
BASE COUNT          536 a          582 c          573 g          646 t
ORIGIN      
        1 ggaaagtata aacgatcgct ctgacagtct cgccgactgc gaccgtgccg ccgatcttta
       61 tcgaattgat gtggggaatg atgaaaattt ccgcggtgtc aaaataattt ccgtccgccg
      121 tttctgcctt gaccgtgatc tttccgggag aaaggggagt tataacggcg ttgttgtcgc
      181 tctgatcgag cttggcaaaa tctgcgccgt cttcaatcga ccaaatgaca gccgtttcgg
      241 agtcgagcgc ggtgagcttt gccgtgagcg ttaatgtggg ctgttcaccc gaatttgtca
      301 tataaatgga agcggcgccc tgaatcttaa cggacggggt ctcgggctct gcctttttac
      361 cctcctgcca cgggaaaatg gggtaacctg agttgacagc gttgctgtca gcgatatagg
      421 cactgccgag tttttctaaa agtccgtcgg gcgaggtaat cttctcgctc gcgccgttct
      481 tgcccgagcc gaaagaatcg gcagcgggat agagcgaata gcaattcttt gtcgccgcac
      541 tgcagtttgc cgagattccg gcaaggaaag tcttatcggg aaaaacagag atctcgccga
      601 tgttatagca gttttcaatt acgctcttcg cggaattttt gccgctgaca tttgagatga
      661 tacccgaggg gtaccaaagt gcctttattt tacctgcgtt atagcaattg actatggtgt
      721 ggccgtcctg agcctgtcct acaatgccgc cgagtgaata aataccgttt atgcttccga
      781 ggttgtagca gttgtctatg gtaacctcaa ccgaagtatc acgtcttgta gtatcgagat
      841 gtcctacaat accgcctgta tggccgctgc ttgcataaag cgagcctacg ttgtagcagc
      901 ccgtgatatt actcttgccg ccgtcgcgca ctgtaccgac tataccgccc atgtaaccgc
      961 tgccgtaaat atcggcaccg ttccagcagt ttataaagtc tgcgccgtta ctccatttgg
     1021 cgataccgcc gaggaacgaa gtgtatatat tttctttgcc gatttcgccg cttgcgacac
     1081 cgacattctt aattacggca ttttggcaga cagttccgaa aagtccgccg cggagagaag
     1141 taagattgtc tatgacatat ccgtcgccgt caaatacacc gtcaaactga tttctgtcag
     1201 agcctatcgg ggtccagttg tcatagtcgc tgaggtcgat attatcggtc atttcaaagt
     1261 aaacaccggt agttgccata agcttggtat ttacgtaata tgacagattg taaaggtcct
     1321 ctgccgtgtt tataaggtac ggtgaagtct cggagcccaa gccctcccac gggattttat
     1381 cctttaccgt aactgttaca aaggtcttga gtttaccgct ctttatacat accttttgag
     1441 ttccgacttc aagtgaatca ccggaaatta tctcataatc cgttacgggc ttttgcgctc
     1501 cgtcgctgta atatgcggta acctccatgc cggcagcgtc aaaagagtcg ccgtcataat
     1561 attcggtctt atcgggtgct tttgtaaccg cgatagaagt gatgtacggc gtatttgagt
     1621 caagctctcc ctcgccgccg acaagctcta ttgccgaaac aagagccgaa taattattta
     1681 cgtatctgcg ttcgttttcg ggaaccgcac ggaattttgc gagtgcgctc tttgccgcag
     1741 acttataatt tgcgtccgtc ggcagaggaa gagcgtctat ggcgcttact gcgtccgtga
     1801 ttgccgcttt ggtttccgcg ctccagtcgc cgcgcatatc ataaagtgct ctcatcttat
     1861 tttcaaatct ccaataagct acgagagcgt aagttgcctg atcgtttgcc atactgttcc
     1921 aaccgttgcc gacaacatgg cagaagccgc cgtttgtatc gcggaatctc agaagtccgt
     1981 caaggagcgt taagccgtcg gaggttataa atctgctgtc tttttggggg tcgattccga
     2041 cagaagtcag cgcaactata acctgagcta tactttcgga gttatcggta ttccaggaag
     2101 taaagcccgc tttatcgttc atcattgagc ctaagcggtc aaatgcttcg tccacgcatt
     2161 gacgaaccgt ttttgaaact tcgtcgcccg aaatttcatt tttatatgta tacacggtat
     2221 cgtcattgta ataaggagcg agcgcctgta ttgccatagc ggtaatatcg acatccgacg
     2281 atgaaccgta accgccgaga acccagccgc cgtatttatt gccgtctacg ccgtcgg
//
LOCUS       DBMDVO010000157         2329 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21883_length_2329_cov_1.376122, whole genome shotgun sequence.
ACCESSION   DBMDVO010000157 DBMDVO010000000
VERSION     DBMDVO010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21883_length_2329_cov_1.376122"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="ACZPE2_03110"
     CDS             complement(<1..605)
                     /locus_tag="ACZPE2_03110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016413 - O-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR1318870.1"
                     /translation="MGSGKLPAVETNSPKRLYYIDILNILSCFAVVVLHCSGGVFYYQ
                     KTRLWFIYMFLQTVAHFAVPVFFMITGANLLDYRKKYDTKTFFKKRAARTLAPFLFWS
                     LLYWVRPFVLKQATAPMSAKGLFLAIFNNEANYVFWFFYCIFAVYMCLPLFSLAADKK
                     NIKTIEYVCVLGFVFNSVLPLVNRFVIPVYCGLTPVIVTGIT"
     gene            945..1031
                     /locus_tag="ACZPE2_03115"
     tRNA            945..1031
                     /locus_tag="ACZPE2_03115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Leu,seq:cag)
     gene            1317..1970
                     /locus_tag="ACZPE2_03120"
     CDS             1317..1970
                     /locus_tag="ACZPE2_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318871.1"
                     /translation="MKNSKEIEPLSQYTESSDTAVVPENRLAGTVGALLFSLAGGVVY
                     FLLYQVGYIAAISGLIGVICAFKGYEVFAKKLSKFGCIISVVMALLVIILAWYCCLAK
                     DVYSAYQSWFADGEIDFTLTFAESLRNAYRFLSEPEISKSYVTDLIIGIFLCALGAVR
                     PIINIVKGNKNKAAEDKNEPAFQAEKYNAPEEKSNAYMPQSMRAASSEKPQDKNGDM"
BASE COUNT          709 a          484 c          490 g          646 t
ORIGIN      
        1 gtaattcccg ttactataac cggagtcagt ccacagtata cgggaattac aaatctgttg
       61 acgagcggca aaaccgaatt gaacacaaag ccgagaacgc acacatactc tatggttttg
      121 atgtttttct tatccgcggc gagcgaaaac agcggcaggc acatatatac ggcaaaaatg
      181 caatagaaaa accaaaatac ataatttgcc tcgttattga atatcgcaag gaaaagcccc
      241 tttgcgctca tgggcgcggt tgcctgcttt aacacaaacg ggcgtaccca gtaaagcagc
      301 gaccaaaaaa gaaacggtgc aagtgttctt gcggcacgtt ttttaaagaa cgtctttgta
      361 tcgtattttt tgcggtaatc aagtaaattt gcaccggtta tcataaaaaa cacgggcacg
      421 gcaaaatgag ctacggtctg caaaaacata tatatgaacc aaaggcgtgt tttctgataa
      481 taaaaaactc cgccggagca atgcagcacc acaacggcaa aacaagacag aatattgaga
      541 atatcgatat aataaagcct ttttgggctg tttgtctcca cggcaggcaa tttaccgctc
      601 cccattttat caactccttt taatgtttat atcataagtt actttgaccg ttttgtcaat
      661 tacgcgcgca taaaaagggt ttgacaacaa cgccgctaaa tggtattata aacagcggaa
      721 attaaaaatc tatactttat cgcttctcgg taatacgagg ttttgatttt taatatcata
      781 tattatatcg tgcggcaaat cggcagatac cgtgtccgat aatgcgccca caccgaaaac
      841 ggtaaaatcg cagaaatgcg tttttatatt tgcggaacaa gccgaaagta agctccgcct
      901 cggatgtcct cgccgtgtaa aattcagagg acaaaactta atatgcgggt atggcggaat
      961 tggcagacgc gcaagattca ggttcttgtg gaggcaactc tgtacaggtt caagtcctgc
     1021 tacccgcacc acgtcggagc aaagcaaact ttgctccgtt tttctttgta tgaaaaatat
     1081 ctgcttacaa actgctgacg tatttatacc gtttcgcgaa agatatattt tattcgccgt
     1141 aacattgcca taagaaatga caacataatc tttttataga caaagctcgc attcttatgt
     1201 ataatcttat cgttgcattc tctgtaaggt tcgccttacc gatgccaagt taaatcaaag
     1261 ctgcggcaaa acggtgcctg caagcatccg atgcccgaaa ggtgatgaat ttcaaaatga
     1321 aaaattcaaa agaaatcgaa cctctctcac aatatacgga gagctccgat actgcggtcg
     1381 ttcccgaaaa tcgtcttgca ggcacggtag gtgcgctcct tttttcactt gcgggaggag
     1441 tagtgtattt tctgctgtac caagtaggtt atattgccgc aataagcgga ctgataggag
     1501 ttatatgtgc gtttaaagga tatgaggttt tcgctaaaaa gctcagcaaa ttcggctgca
     1561 taatttccgt ggttatggct ttgcttgtga taattctcgc ttggtactgc tgtcttgcaa
     1621 aggatgttta cagtgcctat cagtcgtggt ttgccgacgg tgagatagat tttacactca
     1681 cctttgccga gtcgttgaga aatgcttaca gatttctcag tgagcccgaa atctccaaat
     1741 catatgtgac cgacctgata atcggcatat ttctgtgtgc actcggcgca gtaagaccta
     1801 ttataaatat tgtaaaaggc aataaaaaca aagctgccga ggacaaaaac gagcccgctt
     1861 ttcaggctga aaaatacaac gctccggaag aaaagtcaaa tgcgtatatg cctcaaagta
     1921 tgcgtgcggc aagttccgag aagccacagg ataaaaacgg cgatatgtaa ccctcgaagc
     1981 atttaaaatc acatattacg aatacgaaaa gccgaaacgc agcagtaaat tttacttctt
     2041 agtttcggct tttgtttttg tcagaggacg ggttcttgaa accgtcttaa taacattctc
     2101 tttgttttgc tcattattct ttaagttatg ttgttaaacc gggattttta acacataaac
     2161 tcggtcttga ctatcctact aaatcggtag tataataggg atatattttg aaacggagga
     2221 tatgttatga tttcaacaaa agggcgctac gctttgcgcg ttatgataga tataggccgc
     2281 caccaaaatg ccgcaccggt ctctgttaaa gatatatcct caaggcagg
//
LOCUS       DBMDVO010000158         2327 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_21915_length_2327_cov_1.477538, whole genome shotgun sequence.
ACCESSION   DBMDVO010000158 DBMDVO010000000
VERSION     DBMDVO010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21915_length_2327_cov_1.477538"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(94..1209)
                     /locus_tag="ACZPE2_03125"
     CDS             complement(94..1209)
                     /locus_tag="ACZPE2_03125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1318872.1"
                     /translation="MKMNFTAPCIFGLESLVSDELKKMGAENVAAENGRVFFSGDEYL
                     AARANIRSRFSERIMIVVGSFEAHSFEELFEGTKALPWENFIGEFDEFPVKGHSVNSD
                     LFSISDCQSIIKKAIVERLKTKYNVQWFEETGVKFQIDFSIMKNKVHLMIDTTGMGLH
                     KRGYRPVANEAPIKETLAAAMCALSHLRPYHTLYDPMCGSGTILIEGAMLVHNIAPGL
                     KRHFASERFPFIPYEIWESEREKAESEIITESDFKAYGFDINRQTLETAKENAIRAGV
                     ADKIEFARADIRDFAPKSEKGTVITNPPYGERMLSQKEVDDLIRSMGRVFPRKHGWSY
                     SIISPSETFEESFGRKADKRRKLYNGMIKCQLYFYFK"
     gene            complement(1395..1679)
                     /gene="rpmA"
                     /locus_tag="ACZPE2_03130"
     CDS             complement(1395..1679)
                     /gene="rpmA"
                     /locus_tag="ACZPE2_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1318873.1"
                     /translation="MLKINIQLFAHKKGMGSTKNGRDSESKRLGVKRADGQFVLAGNI
                     LVRQRGTHIHPGTNVGKGSDDTLFAKVDGTVKFERLGKDRKKVSVYEVEQ"
     gene            complement(1679..1996)
                     /locus_tag="ACZPE2_03135"
     CDS             complement(1679..1996)
                     /locus_tag="ACZPE2_03135"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016233.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1318874.1"
                     /translation="MITAKFVYSGDIITGFSVSGHSGSAEIGKDIICSAVSSASYMTV
                     NTLTEIMLLNPEIIEKDGFLGIRLSKDEAAAANDLLKGFELHLKELSELYPNYIKIER
                     GAE"
     gene            complement(2002..2313)
                     /gene="rplU"
                     /locus_tag="ACZPE2_03140"
     CDS             complement(2002..2313)
                     /gene="rplU"
                     /locus_tag="ACZPE2_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009982705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1318875.1"
                     /translation="MYAIIETGGKQYKVEKGDVIFIEKLAADENADITFDKVIAVGGD
                     DGLKVGAPYVDGASVSAKVLKNGKSKKVVVFTYKPKKNEKRKMGHRQPYTKVEIADIK
                     A"
BASE COUNT          566 a          563 c          485 g          713 t
ORIGIN      
        1 ctttcccttt accggcagta gggttaatta taaaaatatg cttcataata actctcccat
       61 tataatacaa cttaaaaaag taaaatgcag attttattta aagtaaaaat acaactggca
      121 tttaatcatg ccgttatata gcttacgcct tttatccgct tttctgccga aggactcttc
      181 aaaagtctcc gacgggctta ttatgctgta cgaccaaccg tgctttctcg gaaataccct
      241 tcccattgac cgtattaaat cgtctacctc cttttgcgac agcatccttt caccgtaagg
      301 cgggtttgtg ataaccgtgc ccttttcgga tttcggcgca aaatctctta tgtccgcgcg
      361 tgcaaactca attttgtcgg caactccggc gcgaatcgca ttctcctttg ccgtttcaag
      421 ggtttgcctg tttatatcaa agccgtatgc tttaaagtcg ctttcggtaa ttatttcact
      481 ctctgctttt tcacgctcgc tctcccaaat ttcatacggg ataaacggga accgctcgga
      541 ggcaaaatga cgtttaagcc ccggagctat attgtgcaca agcattgcgc cctctataag
      601 aattgttccg gaaccgcaca tcgggtcgta gagagtgtga tacgggcgca gatgcgacag
      661 agcgcacatt gctgccgcaa gagtctcctt gataggtgct tcgtttgcaa ccgggcgata
      721 gccgcgcttg tgcaggccca tgccggtagt gtctatcatc aggtgaacct tgtttttcat
      781 tatcgaaaaa tctatctgga attttacgcc tgtttcctca aaccattgta cgttatattt
      841 tgttttaagt ctttcgacga ttgctttctt gattatcgac tgacagtcgc tgatgctgaa
      901 aaggtcggaa tttacgctgt gacccttgac ggggaattcg tcaaattctc ctatgaaatt
      961 ttcccacggg agtgcctttg tgccctcaaa cagctcctca aaagaatgcg cctcaaagga
     1021 gcctacgact atcattatac gctcggaaaa tcttgagcgg atatttgcgc gtgccgccaa
     1081 atattcgtct cccgaaaaga acactcttcc gttttcggcg gcgacgtttt ccgcccccat
     1141 ttttttaagc tcgtccgaaa caaggctttc aagcccgaat atgcacggag cggtaaaatt
     1201 catcttcata attagtcctt ttaatatcaa tggtagactt tagtataatt gtttagcctg
     1261 aaaaaatcaa tacccacata ttgcgcttat gttgtcaagg cttggcgaat tgcgtcttct
     1321 tgctgatttt acgggatatt gatcaaacaa aaaaaggcag ggaaaaacct gcctttttcg
     1381 gtaaatgttt aagcttactg ctcaacctcg taaacactga cctttttgcg gtctttgccg
     1441 agtctctcaa acttaacggt accgtcaacc ttagcaaaaa gggtgtcgtc ggagcctttg
     1501 ccgacgtttg taccgggatg gatgtgggtg ccgcgctggc gcacaagaat gttgcctgcg
     1561 agaacgaact gaccgtccgc acgcttaacg ccgagtctct tggattcaga atcacggccg
     1621 ttctttgtgg aacccatacc ttttttatga gcgaacaact ggatgtttat tttaagcatt
     1681 attcagcacc tctttcaatt ttgatgtaat tcggataaag ctcggaaagc tcttttaaat
     1741 gcagctcaaa gccttttaaa aggtcatttg ccgccgcagc ctcgtctttt gagaggcgaa
     1801 ttccgagaaa gccgtccttt tcgattattt cgggatttaa cagcattatt tcggtcagcg
     1861 tgttgacggt catataagaa gccgacgaaa ccgccgagca aattatatcc ttgcctattt
     1921 cggcactgcc cgaatgaccg gatacggaaa agcccgtaat aatgtcaccc gaataaacga
     1981 attttgcggt tatcatttta cttacgcttt gatgtcggct atttcaacct tggtgtacgg
     2041 ctgacgatga cccatcttgc gtttctcgtt ctttttgggc ttgtaggtga aaacaacgac
     2101 ctttttgctc ttgccgttct taagcacctt agcgcttacc gaagcaccgt caacataggg
     2161 tgcaccgact ttgagaccgt cgtcgccgcc gactgcgatt actttatcaa aagtaatatc
     2221 ggcattttcg tctgctgcaa gcttttcgat gaatataacg tcgccttttt cgactttata
     2281 ctgcttgccg cctgtttcga ttattgcgta cattttttta cctgcct
//
LOCUS       DBMDVO010000159         2317 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22054_length_2317_cov_1.155235, whole genome shotgun sequence.
ACCESSION   DBMDVO010000159 DBMDVO010000000
VERSION     DBMDVO010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22054_length_2317_cov_1.155235"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="ACZPE2_03145"
     CDS             complement(<1..764)
                     /locus_tag="ACZPE2_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1318876.1"
                     /translation="MNIFETIYECNINHPSKLALSVTMDDGSTRAYTYGDVFSKVAEY
                     ADRLLSSGVREGDRIAIACEGSPEWTIAFLAVCKIKCTAALIDASLGAEELKSFIDRS
                     DVRAAFLSPKTFGKFTNFPDYKFPVFNVYDCTVFDGCKNSVEEEPTVDPAPEIATIIY
                     SSGTTRRAAGIMHTHDSLIKTTQMTLNVQELVETDRYLAIIPNSHIYGVICLVLGPHI
                     IGADVHYIETLGAEAILKAFKEYKPTVLSAVPKVYEL"
     gene            complement(855..>1723)
                     /locus_tag="ACZPE2_03150"
     CDS             complement(855..>1723)
                     /locus_tag="ACZPE2_03150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1318877.1"
                     /translation="ISTRLRLGDKVDMYINDEFFEKKESTYDFLKASKHLDIIYEDEN
                     IMLLDKKVGVLCHPDNEEYVDTLIGRIKRYLYEKGEYSPDRENSFVPSLVNRIDRNTG
                     GIVIAAKNAESLRILNQKMKDRELHKFYLCVLIGRPKLQSGILKDYLIKDEKKNTVRI
                     YKKQVPSSKEIRTKYRVIDTIDGLSLTEVELLTGRTHQIRAHFAFYGTPLLGDGKYGS
                     NEQNRKYGSYKRQFLYSYKLTFDFTTDAGILSYLDKRTFEAGDVWFKEEFYKGGIVNA
                     TSRHASPKRGES"
     assembly_gap    1724..1733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1734..1879)
                     /locus_tag="ACZPE2_03155"
     CDS             complement(<1734..1879)
                     /locus_tag="ACZPE2_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR1318878.1"
                     /translation="MKSFEIGKNDESQRLDKFIHKAAPLLPQSLLYKYIRTKRIKVNG
                     KRAE"
BASE COUNT          525 a          533 c          543 g          706 t
ORIGIN      
        1 agctcgtaga ccttgggtac ggcggagaga accgtcggct tatattcctt gaatgccttt
       61 aaaatggcct ccgcgccgag agtttctata taatgtacgt ccgctccgat tatgtgcggc
      121 ccgagcacaa ggcatataac gccgtatata tggctgttcg gaatgatggc aagataacgg
      181 tccgtctcga caagctcctg aacgttcagc gtcatctgag tcgtctttat aagcgaatcg
      241 tgagtgtgca tgattccggc ggcacggcgc gttgtgccgg acgaataaat tatagtggcg
      301 atttcgggcg cgggatcgac cgtcggttcc tcctcaaccg aatttttgca gccgtcaaat
      361 acggtgcagt catatacatt aaatacgggg aacttataat cggggaaatt tgtgaatttt
      421 ccgaaggttt tgggtgacag aaacgccgct ctgacatcgg aacggtcaat aaagctttta
      481 agctcctcgg cgccgagaga ggcgtcgatt aaggccgcgg tgcattttat cttgcaaacg
      541 gcgagaaacg ctatcgtcca ttcgggcgag ccctcgcacg ctatcgcaat gcggtcgccc
      601 tctctaactc ccgatgacaa gagcctgtcg gcgtattccg caaccttcga aaatacgtcg
      661 ccgtaggtat atgcgcgcgt cgagccgtcg tccatggtta cggaaagagc gagctttgac
      721 gggtgattta tgttgcactc gtaaatagtt tcaaatatat tcatttctgc ctccacacaa
      781 atgtgataaa tttacgcttt agtgagctga aattatgtaa tattctaaca tctccgcggt
      841 gatatgtcaa tgagctaaga ttcaccgcgc ttcggcgagg cgtgccggct tgtcgcatta
      901 actattccgc ccttgtaaaa ttcctccttg aaccacacgt cgccggcctc aaatgttctt
      961 ttgtcgagat aagagagtat tccggcatcg gtggtgaaat cgaaggtcaa tttgtaggaa
     1021 tacagaaatt gcctcttgta agagccgtat ttgcggtttt gctcgttact gccgtatttt
     1081 ccgtcgccga gcagcggagt gccgtaaaag gcgaaatgcg cgcgtatttg gtgtgttctg
     1141 cccgttaaaa gctctacctc ggtaagcgaa agaccgtcta ttgtgtctat aactcgatat
     1201 tttgtgcgta tttcctttga ggacggcacc tgctttttgt atatgcgcac ggtgtttttc
     1261 ttctcgtcct ttatgagata atcctttaaa attccgcttt gcagcttggg tctgccgatt
     1321 aaaacgcaga gataaaactt gtgtagctcc ctgtccttca tcttttgatt cagtatgcgc
     1381 agcgactcgg cgttttttgc cgcaattact attccgcccg tgttgcggtc tatgcggttc
     1441 acaagagacg ggacaaatga gttttctctg tcgggggaat attcaccttt ttcgtacaga
     1501 taacgcttta tgcggcctat gagcgtgtca acatattcct cgttgtccgg atgacacaga
     1561 acaccgacct ttttgtcaag gagcattatg ttttcatcct cgtaaattat gtcgaggtgc
     1621 ttcgatgctt ttaaaaagtc gtaagttgac tctttttttt caaagaactc gtcgtttatg
     1681 tacatatcca ccttgtcgcc gaggcgaagc cgcgttgaga tttnnnnnnn nnnatttcgg
     1741 ctcttttccc gttaaccttt atgcgctttg ttcttatata cttatataag agcgattgcg
     1801 gcaaaagcgg ggctgccttg tgtatgaatt tgtcaagtct ttgcgactcg tcgttttttc
     1861 cgatttcaaa gcttttcatt gtgtgcttct ccgtgatttt cgtatactat tatatatagg
     1921 cgttttttta cgccggattt cggtggatgt ttttcgggtt ttgagcttgc ttttttgccg
     1981 ttttgtgccg tttttcggta caagcgcgaa aaattctccg ccgccttcgt tctctgccga
     2041 aaaatgatgc gccatcccgt ctgccgagat gaaaattcgc accgcttttg tgcggaaaac
     2101 gctatatatt tattatacca taacacagcg aaaattacca taaaaacacc tcggcttgac
     2161 gacggttgcc gtccgaatcg ttaagtcacc gccggagttt tttctctttg taaactgtca
     2221 atttcgcgct cgaagataag ttgtgccgaa aattgccgta agtgtcctca aaatgggacc
     2281 ttatcccata aaacagcctg tttttaatcg gaaaatc
//
LOCUS       DBMDVO010000160         2291 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22415_length_2291_cov_1.325114, whole genome shotgun sequence.
ACCESSION   DBMDVO010000160 DBMDVO010000000
VERSION     DBMDVO010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22415_length_2291_cov_1.325114"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..1093)
                     /gene="galE"
                     /locus_tag="ACZPE2_03160"
     CDS             complement(74..1093)
                     /gene="galE"
                     /locus_tag="ACZPE2_03160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1318879.1"
                     /translation="MAILVTGGAGFIGTHTCIELINAGYEPIVVDNYYNASPKALERV
                     EEIVGKKVKFYECDVRDKKKLDSVFKENKIDAVIHFAGLKAVGESCHKPLEYYDNNIG
                     GTLALCEVMRDNGCKKIVFSSSATVYGMNNVSPLTEDMKTGGTTNPYGTTKYMIEIIL
                     EDLYKADNSWDVTLLRYFNPIGAHKSGLIGENPNGIPNNLMPYITQVAIGKLDCLSIY
                     GNDYPTRDGTGVRDYIHVVDLALGHVKAVEQLKSSNGGVRIYNLGTGKGYSVLDIVKA
                     FEKATGVKINYKIAPRRDGDIATCYSDPSKAKKELGWEAKRDLEEMCEDSWRWQKNNP
                     NGFDD"
     gene            complement(1145..>2291)
                     /locus_tag="ACZPE2_03165"
     CDS             complement(1145..>2291)
                     /locus_tag="ACZPE2_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1318880.1"
                     /translation="SSVGIEISGEAAAEPEAEKTPGKGKKVLVSILVIILALAVAAGT
                     FFLVKYQRDKKAAEALANTAPETTSEETTVPPEISKDTVPLSVFMRNEAVNGIDKDDL
                     ARAAEDAASAASQGDNAANENALPEYIVLNGVKTEAKKALAKIVAADVDDSYNSEAIK
                     ADAVAVYTYLKYRNTNFNVSGLNAAETVSDNILNAVSEVFGEYVVYNGQPAFTPTFKL
                     SAGKTTSADVVFGNSFPYLKTVDSASDKNADGYKTEITLTSGELKELANKFDSSINLS
                     GSAKDWVKVTKHDGAISTGVGYVETVNVGGKEISGYKFACELLENKIPSYCFAVSYTS
                     SGDTFKITSYGSGFGVGMSLAGANKMAADGSTYAQILAKYYPGTNLS"
BASE COUNT          492 a          542 c          566 g          691 t
ORIGIN      
        1 ggcaacaatg attcctactg taagactttt tttgtttttc atggtgttac ctcctttttc
       61 ggggggagaa atatcagtcg tcaaaaccgt tcggattgtt tttttgccaa cgccatgaat
      121 cctcgcacat ttcctcgagg tcgcgttttg cctcccaacc gagctctttt ttagcctttg
      181 acgggtcgga gtagcaggta gctatatcac cgtcacggcg aggagcgatt ttataattga
      241 ttttaacgcc tgtggctttt tcaaatgctt taactatatc taaaacgctg taacccttgc
      301 ctgtgccgag attataaatt cttacgccgc cgtttgagga tttcagctgc tctacggctt
      361 ttacgtgacc gagagccagg tcaaccacgt ggatgtaatc gcgaacaccc gttccgtcgc
      421 gggtggggta gtcgttgccg taaatcgaga ggcagtcaag cttgcctatt gcaacctggg
      481 ttatatacgg cataagattg ttcgggattc cgttggggtt ttcacctatg aggccgctct
      541 tgtgagcacc tatcgggtta aagtagcgaa gcagcgtaac atcccagctg ttgtctgctt
      601 tatagagatc ctcgaggatt atctctatca tatactttgt ggtaccgtag ggattggttg
      661 taccgccggt tttcatatcc tccgtgagcg gcgaaacatt gttcataccg tagacggtag
      721 cggaggagga aaatacgatt tttttgcagc cgttgtctct cataacctcg cagagcgcaa
      781 gagttccgcc gatgttgtta tcgtagtact caagcggctt gtggcaggat tcgcctaccg
      841 ccttgaggcc tgcaaaatgt atgacggcgt ctattttatt ttccttaaag acggagtcaa
      901 gcttcttttt gtcgcgaacg tcgcactcat aaaacttaac cttttttccg actatttcct
      961 caacgcgctc gagtgccttg gggcttgcgt tgtagtagtt atcaaccact atcggttcat
     1021 atcctgcgtt aataagctct atgcaggtat gggtgcctat aaatccggca cctccggtta
     1081 ctaatattgc catttaaaat gtcccccttg ataatatata caaaatatga cggcttaaaa
     1141 agtgttatga aagatttgtt ccgggatagt attttgcgag tatctgcgcg taggtcgagc
     1201 cgtcggccgc cattttgttt gcaccggcaa ggctcatacc gacaccgaaa ccgctgccgt
     1261 atgaggttat cttaaaggtg tcgccggaag aggtgtagga tacggcaaag cagtatgacg
     1321 gaatcttgtt ttcgagaagc tcacaggcga atttatatcc gcttatttcc ttaccgccta
     1381 cgtttacggt ttcgacgtat cctactcccg tgcttatggc gccgtcgtgc tttgtaacct
     1441 ttacccagtc cttggcggag ccggagagat ttatcgagct gtcaaattta tttgcaagct
     1501 cctttaattc accggatgtc agagtaatct ccgttttgta gccgtctgcg tttttatcgg
     1561 atgcgctgtc taccgtttta aggtagggga aggaattccc gaaaacaacg tctgccgagg
     1621 tggttttacc tgccgacagc ttaaatgtgg gagtgaatgc cggttgaccg ttatatacga
     1681 catattcgcc gaatacctcc gaaacggcat tcagtatgtt gtcgctcacc gtttctgcgg
     1741 cattaagacc gctcacgtta aagtttgtgt tgcggtattt caaatatgta taaacggcaa
     1801 ctgcatcggc cttaatggct tcggaattgt agctgtcgtc cacatccgcg gccacaattt
     1861 ttgcgagtgc cttttttgcc tccgttttta cgccgttgag cacaatgtat tcggggagag
     1921 cgttttcgtt tgccgcattg tcaccctgag aagcggcgct tgcggcgtcc tcggccgctc
     1981 tcgcaaggtc gtccttgtca attccgttaa ccgcttcatt gcgcataaaa acagagagcg
     2041 gaaccgtatc cttggatatt tccgggggaa cggtcgtttc ctcggaagtc gtttcgggcg
     2101 cggtgtttgc gagcgcctcg gcggcttttt tgtctctttg atatttcaca aggaaaaatg
     2161 tgcctgccgc aacggcaaga gcgagaatta ttacaagtat tgaaacaagg acttttttcc
     2221 ctttgccggg agttttttcg gcttcgggct ctgctgcggc ttcgccggaa atctctatgc
     2281 cgacggagct t
//
LOCUS       DBMDVO010000161         2291 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22418_length_2291_cov_1.084018, whole genome shotgun sequence.
ACCESSION   DBMDVO010000161 DBMDVO010000000
VERSION     DBMDVO010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22418_length_2291_cov_1.084018"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="ACZPE2_03170"
     CDS             <1..1756
                     /locus_tag="ACZPE2_03170"
                     /inference="COORDINATES: protein motif:HMM:NF027658.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminase domain-containing protein"
                     /protein_id="HIR1318881.1"
                     /translation="VTPLCTTYVFEKHDVQLKVDFWTPAFPDDLLLLSLPCAFIDYEV
                     TILDKRPHSVSISLLVDENFCYDSEKGKEIIGDAVKTDSSYYAYMRQNEQNVLEYSGD
                     FNAVNWGTVYVTGGFVSFGKPTIKRNKRDGFVNYIHSEHKAYEPVSDKFCAHDTLFFD
                     DGFSIEYMGEKLRGYWTEKFPDAVSALKYCDEKHDELRKQVSLRNTRILRDGQRFGDD
                     YCMLLSAAYREVIASHKLVKTDDGKLLYVSKNCTAGGFAAETDVTYASSPLFLLYNPS
                     LVFAELNGICDFARSAAWKFDFAPHDIGTYPIADGQTYGLKADFDGDKAKVYSTSDNI
                     YDEEEQMPVETSGDVLILAYAATVLSGDRSFIAENKDLLLKWGDYLAETGNDIANQKN
                     ADDYAKAISGSVNLAVKSCIALRCCGEICKMLDSDGEKYLKAASENAADILKRDEGRE
                     CLSFTLLKKESWSLKYNLVWNYIFGFDLFPLKTAKNEIARYIKIKNEYGLPLGPRRDY
                     ARTDWTMWACALDDTGFMTQKLSVDIMRMLDHTEDKYPFTDWYDTKSAKSKGRYHRPV
                     QGGLWMPVLAKKWNEILP"
     gene            complement(1864..2142)
                     /locus_tag="ACZPE2_03175"
     CDS             complement(1864..2142)
                     /locus_tag="ACZPE2_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318882.1"
                     /translation="MKRDLGKADEIFDVPAINFFTSGNDYFGSSGSKFRYRISHTDEN
                     IVGEVWHEDLCYEKCTVTDKKEDFPLTPDGLTNCIKWLYEKYESTYEA"
BASE COUNT          618 a          476 c          554 g          643 t
ORIGIN      
        1 ggtcactccg ctttgcacta catatgtgtt tgaaaagcac gacgttcagc tcaaggtcga
       61 cttttggact ccggcttttc ccgatgacct tttgcttttg tctttgccgt gcgcctttat
      121 agattatgag gttacgattc ttgacaaaag gccgcattcc gtttcgatat cgcttcttgt
      181 ggacgagaac ttttgctatg acagcgaaaa gggaaaggaa attatcggcg atgcggttaa
      241 gacagacagc tcatactacg catatatgag gcaaaatgag cagaacgttc ttgagtattc
      301 gggtgatttc aatgccgtaa actggggaac ggtttatgtt acgggcggct ttgtctcatt
      361 tggcaaaccc acaattaagc gcaataagcg tgacggcttt gtaaactaca ttcacagcga
      421 acataaggca tatgagcctg taagcgacaa attttgtgcg cacgacacat tgttttttga
      481 cgatggcttc agcatagaat atatgggcga aaagctcaga ggatattgga ccgagaaatt
      541 tcccgatgcc gtttcggcac tcaaatactg cgacgaaaaa cacgacgaat tgaggaaaca
      601 ggtttctttg cgaaatacgc ggattttgcg cgatggtcag cgtttcggcg atgattactg
      661 tatgcttttg tcggccgcgt acagagaggt catagcgtct cataagcttg taaaaaccga
      721 tgacggaaag cttttgtatg tttccaaaaa ctgcaccgcc ggcggatttg ccgccgagac
      781 cgatgtaacg tacgcgtcgt caccgctttt ccttttgtat aatccgagtc ttgtgtttgc
      841 cgagcttaac ggtatatgtg attttgcgcg ttctgccgcg tggaaatttg attttgcgcc
      901 gcacgatata ggcacatatc ccatagctga cgggcagaca tacggtctta aagccgactt
      961 cgacggcgac aaggctaagg tatattcgac ctcggacaac atatatgacg aggaggagca
     1021 gatgcccgtc gaaacaagcg gcgatgtact tatactcgct tatgccgcga cggttctttc
     1081 cggcgacagg agctttattg ccgaaaataa agatttgctt ttgaagtggg gagattatct
     1141 tgccgaaacg ggcaacgata tagctaacca aaagaatgcc gacgactatg caaaggcgat
     1201 atcgggcagc gtaaatcttg ccgttaaatc ctgcatagca ctgcgctgct gcggagaaat
     1261 atgcaaaatg cttgattccg acggagaaaa atatcttaaa gccgcctctg agaacgcggc
     1321 agatattctc aaaagagacg agggcaggga gtgcctttcg tttactctgt taaagaagga
     1381 gagctggagc cttaagtata atcttgtgtg gaattatata ttcgggtttg atttgttccc
     1441 gctcaaaacg gccaaaaatg aaattgcgcg atatatcaaa attaaaaacg aatacggcct
     1501 gccgctcggg ccgcgtagag actatgcgcg taccgattgg acgatgtggg cgtgcgccct
     1561 tgacgatacc ggatttatga cccaaaagct gtcggtggat ataatgcgta tgcttgacca
     1621 taccgaagat aaatatccgt ttacggattg gtatgataca aagagcgcaa agtcaaaggg
     1681 aagatatcac cgacctgttc agggaggact ttggatgcct gtactcgcga aaaagtggaa
     1741 tgaaattttg ccgtgagata aaatgcccgt gcgttgccga cagcgatata taataagcaa
     1801 taacaaataa acggccgccc tcggaaatgc ttccgatggc ggctttctct gttttgcttg
     1861 attttaggct tcgtatgtgc tttcgtattt ttcgtaaagc cattttatgc agtttgtaag
     1921 accgtccggc gtcaacggga aatcctcttt tttatcggta acggtgcatt tttcgtagca
     1981 gagatcctcg tgccatactt caccgactat gttctcgtcg gtgtgcgata ttctgtatct
     2041 gaatttgctg ccgctgcttc cgaaataatc atttccgctt gtgaaaaagt ttattgcggg
     2101 gacgtcaaat atttcatcgg ctttgcccaa atcccttttc atatgttata tacctctctt
     2161 ttggtgtgtt attactattt tgattataat attaacggcg aaattaagca agaaatttat
     2221 caaattagca ctctgagtag tagagtgcta atagtttaca atttgttcat atttatataa
     2281 caaacggata a
//
LOCUS       DBMDVO010000162         2286 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22484_length_2286_cov_1.138673, whole genome shotgun sequence.
ACCESSION   DBMDVO010000162 DBMDVO010000000
VERSION     DBMDVO010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22484_length_2286_cov_1.138673"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(33..1310)
                     /gene="serS"
                     /locus_tag="ACZPE2_03180"
     CDS             complement(33..1310)
                     /gene="serS"
                     /locus_tag="ACZPE2_03180"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1318883.1"
                     /translation="MLDIKLVRENPDLVKENIKKKFQEEKLALVDEVIELDKKFRESK
                     QRADELRASRNKISKEIGGLMAKGLREEAEKTKQKVAHLAKELEELEGSEETLSAEIK
                     KRMMVIPNIIGDDVPIGKDDSENVELQRFGEPYVPPFEIPYHVDIMEKLHGIDLDSAR
                     KTSGNGFYYLCGDIARLHSAVLSYARDFMIDRGFTYYVPPFMIRGNVVNGVMSFSEME
                     NMMYKIEGEDLYLIGTSEHSMIGKFIDTITPEAELPKTLTSYSPCFRKEVGAHGIEER
                     GVYRIHQFEKQEMVVVCKPEDSDMWYEKLWNNTVDFFRSLDIPVRTLECCSGDLADLK
                     VRSLDVEAWSPRQKKYFEVGSCSNLGDAQARRLGIRIKGENGNYFAHTLNNTVVAPPR
                     MLIAFLENNLNEDGSVRIPEPLRMYMGGKDVIK"
     gene            complement(1341..2204)
                     /locus_tag="ACZPE2_03185"
     CDS             complement(1341..2204)
                     /locus_tag="ACZPE2_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1318884.1"
                     /translation="MAARKGGLGKGFEALMQSNTMDDELGEKATLSINDIEPNRGQPR
                     KTFSEESLSELADSIREHGVLQPLLVRPMPDGSYRLVAGERRFRAARLAGLYEVPVTV
                     REMTDEEESVFALIENLQREDLNAVEEAEGIKTLIDTFGFTQEQAAQKLGKSRTAVTN
                     SLRLLNLPDSVGSLLKDGKISMGHARALLAVEDEAEMKSIADTIVKNGISVRETERLA
                     KKAGNGGTAAVKRKKKRDTFYDEVELALAGVLARRVKVYNGKGSGGSIEIEFFGKDDL
                     KKLAEQLEALE"
BASE COUNT          481 a          626 c          507 g          672 t
ORIGIN      
        1 caatttgtca ataccggttt cgggtaagac gcttatttta tgacgtcctt accgcccata
       61 tacattctga gcggctcggg gatgcgcacg gagccgtcct cattgagatt gttctcaagg
      121 aaggctatga gcattctcgg aggagcaacg acggtgttat taagagtatg tgcaaaatag
      181 ttgccgtttt cgcccttaat ccttatgccg agtcttcttg cctgagcgtc gccgaggttt
      241 gagcatgagc caacctcaaa atattttttc tgacgcggag accaagcttc aacgtcaagg
      301 cttctgacct tcaaatcggc aaggtcgccg gagcagcact caagggtgcg caccggtata
      361 tcgagcgagc ggaagaaatc aacggtgttg ttccagagct tttcgtacca catatcgctg
      421 tcctcgggct tgcagacaac aaccatttcc tgcttttcaa actgatgtat gcgatatacg
      481 cctcgctcct ctatgccgtg cgcgccgacc tccttgcgga agcagggcga atagcttgtg
      541 agagttttgg gaagctccgc ttcgggagtt atggtgtcta taaacttgcc tatcatcgaa
      601 tgctccgacg tacctataag gtagaggtcc tcgccctcga ttttatacat catattttcc
      661 atttcggaga agctcataac accgttaaca acatttcctc ttatcataaa cggcggaaca
      721 taatatgtga agcctctgtc tatcataaaa tcgcgcgcat atgagagcac cgcagagtgg
      781 agtcttgcaa tatctccgca gagatagtaa aagccgttgc cgctcgtttt tcttgcggaa
      841 tcaaggtcga ttccgtggag cttttccata atgtctacgt gatagggaat ctcaaacggc
      901 ggaacatacg gctcaccgaa gcgttgaagc tctacgtttt cgctgtcgtc cttgcctatc
      961 ggaacatcgt cgcctatgat gttaggtata accatcattc tctttttgat ttctgcggag
     1021 agtgtttcct cgctgccctc gagctcttca agctcctttg cgagatgcgc aaccttttgt
     1081 tttgtctttt ccgcttcctc gcgcagaccc tttgccataa ggccgcctat ttccttgctt
     1141 atcttgttcc ttgaggcgcg gagctcatcg gctctctgct ttgactcacg gaactttttg
     1201 tcaagctcta ttacctcgtc tacaagcgcg agcttttcct cctggaattt ctttttgatg
     1261 ttctctttta cgagatccgg attttctctt acaagcttaa tatcaagcat ttttcttcct
     1321 ccgaaaaatt aaaattcaaa ttattcaagt gcttcaagct gctctgcaag cttttttaaa
     1381 tcgtccttgc cgaagaactc tatttctatg ctgccgcccg aacccttgcc gttatatacc
     1441 ttaaccctgc gtgccaaaac tccggccagc gcaagctcga cctcgtcata aaaggtgtcg
     1501 cgttttttct ttctctttac cgccgccgtg ccgccgtttc cggctttttt ggcaagtcgc
     1561 tccgtctcgc ggacggatat gccgtttttg actatcgtat cggctatact tttcatctcg
     1621 gcctcgtcct cgacggccaa aagcgccctc gcgtgaccca ttgagatttt accgtccttc
     1681 aaaagggaac cgacgctgtc gggaagattc aaaagcctga gtgaatttgt aaccgccgta
     1741 cgcgattttc cgagcttttg agccgcctgt tcctgagtga agccgaaggt gtcgataagg
     1801 gtttttatac cctccgcctc ttctacggca ttgaggtcct cacgctgcaa attctcgata
     1861 agagcaaaca cactctcctc ttcgtcggtc atctcgcgaa cggtaacggg aacttcataa
     1921 agccccgcaa gccttgccgc gcggaagcga cgctcgcctg cgacaagacg gtagctgccg
     1981 tcgggcattg gtcttaccaa aagcggctgc aaaaccccat gctctcttat gctgtcggcg
     2041 agctccgaaa gagactcctc gctgaaggtc tttctcggct gaccgcggtt cggctcaata
     2101 tcgtttatcg aaagcgtcgc cttttctccg agctcgtcat ccatggtgtt tgactgcatt
     2161 agtgcctcaa aacccttgcc gagcccgcct tttctggctg ccatcttatt tcctcccctg
     2221 ctttgtttcg ttgtattttt caacgtcctt tttattctcg gtctcaaaaa gacctttctg
     2281 catata
//
LOCUS       DBMDVO010000163         2285 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22496_length_2285_cov_1.064560, whole genome shotgun sequence.
ACCESSION   DBMDVO010000163 DBMDVO010000000
VERSION     DBMDVO010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22496_length_2285_cov_1.064560"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            311..835
                     /locus_tag="ACZPE2_03190"
     CDS             311..835
                     /locus_tag="ACZPE2_03190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1318885.1"
                     /translation="MELIRLRDSRGKIFDTAMKLYEKSFPKYERRSYDAQQRAMYDEQ
                     FHFDLIYDEGEFIGEVLYWDTDSFIYIEHFCVLPEKRNRKYGERTLSMLQGCDKTIIL
                     EIDPPNDEISVRRRGFYERNGFKLNDFEHFAPAYTRGGSDFELKIMTYPGKISKELYD
                     DFYEFLCAKVKMYI"
     gene            1043..>2285
                     /locus_tag="ACZPE2_03195"
     CDS             1043..>2285
                     /locus_tag="ACZPE2_03195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1318886.1"
                     /translation="MGAKKTVMLTGASGHMGFHGFKELYAKKDKFNLVLLLRGSEKNR
                     VKFKDFENDPSVRIVWGDLTNYEDVLNAVTGSDYILHVGGMVSPAADWKPYRTQKTNI
                     GAAQNICKAVLAQPDPDAVKVCYIGTVAETGGRNYPIHWGRCGDPLKVSVYDHYAVSK
                     CIAERVFVESGIKNWVVMRQSGILYPNILKNMDPIMFHVPINGVLEWCTVEDSGRLLA
                     NLCDEDAKGNLGSDFWNHFYNIGSGKEYRISNYEFECLLLGTLGLAGPEKLFDPNWFT
                     TKNFHGQFYADGDKLENFLHFRENLPVKDYFNRLADQVEFYFKIPRYLPKNLVAACAK
                     PFMKKIAKTPDFGTLDWVEKNNKQRLDIYFGGMDEWKKLPSKWEDFDIIKFDKDNSAA
                     EQFKLDHGYDETKPEAELDIED"
BASE COUNT          674 a          435 c          522 g          654 t
ORIGIN      
        1 gggttgactg ccgggcaaaa aatgaagaaa tggttgactg gattgctatt caatatccga
       61 agattaaaaa ggtgtttcag cagttagatt gtgatgaata attatgaata agttactgat
      121 tgcaaaaatt tttcaataaa ttctacaata aatagtttta tccgacagct taagttgcac
      181 catttgtgtc atccgatgac gtcttgtccg aatgtcgttt ttgtgataaa atatatatta
      241 ttaaaaatat gtttgtaggg cgatataaga taaaaccgcc tgcaaaaata ctgattatac
      301 ggagaattat atggagctta tacgtttgag agacagcagg ggaaaaatat ttgataccgc
      361 aatgaagctc tatgaaaaaa gcttcccgaa atacgagcgg aggtcatatg acgcacagca
      421 gagggccatg tatgatgagc agttccattt tgaccttata tatgatgagg gcgagtttat
      481 aggcgaagtg ctttattggg ataccgacag ctttatatat attgagcatt tttgcgtttt
      541 accggaaaag cgcaaccgca agtacggaga gcgcacactc tcgatgctgc aaggctgcga
      601 taaaaccatt attttggaaa tcgacccgcc aaatgacgaa atatccgttc gccgtcgcgg
      661 attttatgag cgaaacggat ttaaattaaa cgactttgag cattttgcac cggcttacac
      721 aaggggaggc tctgattttg agcttaaaat tatgacgtat cccggtaaaa tctcaaagga
      781 gctgtatgat gatttttatg agttcctttg tgccaaggta aaaatgtaca tttgacggcg
      841 gtgcttttaa cggcgtgagc tgtaaaggtt gaattccgcc gtgatacata ttgaattaaa
      901 cgtcgctgtt aaaaaatgcg cgcgtttctt tgcaaaaata aatatctgtt tacagaatat
      961 attgattttt ttactctttt gttgacattg tacataaaaa tgttataata aaagcactat
     1021 gatttaaatg aggtggaaca atatgggagc taaaaaaacc gtaatgctga ccggtgcctc
     1081 cggtcatatg ggtttccacg gttttaagga actttacgct aagaaagata agtttaatct
     1141 tgttctttta cttcgcggaa gtgaaaaaaa ccgtgttaaa ttcaaggatt ttgaaaatga
     1201 tccttcagtc agaatagtat ggggcgatct tacaaactat gaggatgttc tcaacgcagt
     1261 aacaggttct gactatattc ttcatgtagg cggtatggtt tctccggcag ctgactggaa
     1321 accgtacaga acacagaaaa caaacattgg tgctgctcag aatatctgca aggcagttct
     1381 tgctcagccg gatcccgacg ctgttaaggt ctgctacata ggcaccgttg ccgaaacagg
     1441 cggtcgtaac tatcctatcc attggggacg ttgcggcgat ccgctcaagg tttcggtata
     1501 tgaccattat gctgtttcta agtgcatagc agagcgtgta ttcgttgaga gcggtattaa
     1561 gaattgggtt gttatgcgtc agtcgggcat actttatccc aacattctta aaaatatgga
     1621 tcctattatg ttccacgttc cgatcaacgg cgttcttgag tggtgcaccg ttgaggattc
     1681 gggtcgtttg cttgccaacc tctgcgatga agatgcaaaa ggcaacctcg gttccgattt
     1741 ctggaatcac ttctacaata taggttcagg taaggaatac agaatttcaa actacgagtt
     1801 tgagtgcctt cttctcggta ctctcggtct tgcaggcccc gaaaagctct ttgaccccaa
     1861 ctggtttaca acaaagaact tccatggtca gttctatgca gacggcgata agcttgaaaa
     1921 cttccttcac ttccgtgaga acctccccgt taaggattac ttcaaccgtc ttgcagatca
     1981 ggttgaattc tactttaaaa ttccgagata tcttccgaaa aacctcgttg cagcttgcgc
     2041 gaagcccttt atgaagaaga ttgccaagac tccggacttc ggtactcttg actgggttga
     2101 aaagaacaac aagcagcgtc ttgacatcta cttcggcgga atggacgagt ggaagaagct
     2161 cccgtcaaag tgggaagatt tcgacatcat caagttcgat aaggacaaca gcgcggctga
     2221 gcagtttaag cttgaccacg gttatgatga gacaaagccc gaagcagagc ttgacattga
     2281 ggata
//
LOCUS       DBMDVO010000164         2271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22715_length_2271_cov_0.863134, whole genome shotgun sequence.
ACCESSION   DBMDVO010000164 DBMDVO010000000
VERSION     DBMDVO010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22715_length_2271_cov_0.863134"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="ACZPE2_03200"
     CDS             <1..1592
                     /locus_tag="ACZPE2_03200"
                     /inference="COORDINATES: protein motif:HMM:NF046269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR1318887.1"
                     /translation="SWKHSDEGYYQNTLVHTLYIEPGKSHTEYAVISYGGAEYETPED
                     YEKIYLSASGSVEKLSYNDSGKKYEFSNRLLRAAMFQNTVYPLYHHGEYVIHHTPGKR
                     WDSFYTWDSGFIGLDMLEFSPKIADYILDLYLSDKDNKDYAFLLHGSPVPVHLYQYYE
                     MLQKASDKSELYDYYDRAKMFYDFLAGKINGSTTAKFKSGLTTTFEYFYNCSGMDDLP
                     PQVLMYKNNLQLKTAPCISSSQVIRTAKLLSVIAEHLGKSEDVKAYSEDIKRISNGLQ
                     KYAWDDEAGYYSYVIHDENGEAKEQLRSDSGENMNKTMDGIYPLIAGITTDEQTGRIL
                     SHLESEDEMMSKVGISAVNMKAGYYATNGYWNGNVWFSHQWFVWKTMLDIGEADFAYK
                     IAKVALDSWKREVEYSYYTFEMLSITTGRGGWFHNFGGLSAPVNIWASAYFKAGTFNL
                     GFDSFCENYSFENDFTHFSADVSHYNGKDSIMLVVMNDKNNYVVTVDGEKAVFNERSK
                     GTFEIKLPSDKKRVIIEIQLV"
     gene            complement(1580..1903)
                     /locus_tag="ACZPE2_03205"
     CDS             complement(1580..1903)
                     /locus_tag="ACZPE2_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318888.1"
                     /translation="MKKALLKCIAASVAALTLLGAVTVIILKSPKKKSSFTESEETTV
                     AALYTITDYNGKIALFKRGYSMPVEIFDVNTNSLPQSDRELIIVGVTAYSDEEAQKLV
                     EDYTS"
     gene            2051..>2271
                     /locus_tag="ACZPE2_03210"
     CDS             2051..>2271
                     /locus_tag="ACZPE2_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318889.1"
                     /translation="MTQKRRAVTKRVPKNRKKRSKGATVLLSVLFTFLLTAVFVCLYL
                     LVFMVSYVNGDSKINLEEYKENQDQTTII"
BASE COUNT          693 a          410 c          488 g          680 t
ORIGIN      
        1 tcagttggaa acacagcgac gagggatact atcagaatac tcttgtccac accttgtaca
       61 tagagccggg gaaaagtcat accgaatatg cggttatttc gtatggcgga gcagaatatg
      121 aaacgccgga agattatgaa aagatctatt tatcggcgtc cggctcggtt gaaaagctaa
      181 gctataatga cagcggaaaa aaatatgagt tttcaaaccg ccttttgcga gctgcaatgt
      241 tccaaaacac cgtgtatccg ctttatcatc acggagaata tgttatacat cacactcccg
      301 gcaagaggtg ggattccttt tacacatggg attcgggctt tatagggctt gatatgcttg
      361 aattttcacc gaaaatagcg gattatatcc tcgatttgta tttgagcgat aaggataata
      421 aggactatgc gtttttgttg catggttctc ctgtgcccgt ccatctttat caatattatg
      481 aaatgctcca aaaagcatct gataaatccg agctgtatga ttattacgac cgggcaaaga
      541 tgttttatga tttccttgcg ggaaaaataa acggttcaac caccgctaaa tttaagagcg
      601 gtcttacaac tacatttgag tatttttaca attgttccgg tatggacgat cttccgcctc
      661 aagttctgat gtacaaaaat aatttgcagc ttaaaaccgc accgtgtatt tcttcttctc
      721 aggttataag aacggcaaag cttctttccg tgatagcaga gcatctcggt aaatccgaag
      781 atgtaaaagc ctattcggaa gatataaaga gaatatcaaa tgggcttcag aaatatgcgt
      841 gggatgatga ggcggggtat tactcatatg taattcacga tgaaaacggc gaagcaaaag
      901 aacaactgcg ctctgattcg ggcgaaaata tgaataagac catggacggt atatatcctc
      961 ttatagccgg aataactacg gacgagcaga ctggcagaat tctgtctcat ttggaaagcg
     1021 aagatgagat gatgagcaag gtcggtataa gcgccgttaa tatgaaggcc ggctattatg
     1081 ccactaacgg ctattggaac ggcaacgtat ggttttcgca tcaatggttt gtttggaaaa
     1141 ccatgttaga tataggtgag gctgattttg catataaaat agccaaggtc gcgctcgatt
     1201 cgtggaagcg tgaggtggag tattcgtact atacttttga aatgctttcg ataactacgg
     1261 gcagaggagg atggttccat aatttcggag gcctttccgc tcccgtgaac atttgggctt
     1321 ctgcatactt taaggcgggc acgtttaatc tcggctttga cagcttttgt gaaaattaca
     1381 gcttcgaaaa cgatttcact catttttccg cagacgtttc gcattataac ggtaaagaca
     1441 gcataatgct tgttgttatg aatgacaaaa ataattatgt ggtaacagtt gacggagaaa
     1501 aagccgtttt taacgagcgg agcaaaggaa catttgaaat aaaattaccc tctgataaaa
     1561 aacgtgtaat aattgaaatt cagcttgtgt aatcctctac aagcttttgt gcttcttcat
     1621 cggaataagc cgtaacacct actattataa gctccctgtc gctttgcggc agggaatttg
     1681 tatttacgtc aaagatttcc accggcatag agtatccgcg tttaaataaa gctattttac
     1741 cgttatagtc ggttatggtg taaagtgctg caacggtagt ttcttcactt tccgtaaagc
     1801 tgcttttctt tttaggagat tttaaaatta ttactgttac tgcacctaat aaagtcagag
     1861 cagccaccga tgcggcgata catttaagta aggctttttt cattttattc acttccgtat
     1921 attatttttg acaagaatag gaaaaatata atattttttc aaaattatga tgaaataatc
     1981 aacggtttgt gttacaataa atcagtatag ttatatcgta tccgatcttt taaacggagg
     2041 actttaaaat atgactcaaa agcgacgcgc agtcacaaag agagtaccta aaaacagaaa
     2101 aaaacgcagt aagggcgcaa cggttttgtt gtcggttctt tttacctttt tgcttaccgc
     2161 ggtttttgta tgcttatatc ttcttgtgtt tatggtgtct tacgtaaacg gcgacagtaa
     2221 gataaatctt gaggaatata aagagaatca agatcaaacc acaattatat a
//
LOCUS       DBMDVO010000165         2267 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22766_length_2267_cov_1.278855, whole genome shotgun sequence.
ACCESSION   DBMDVO010000165 DBMDVO010000000
VERSION     DBMDVO010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22766_length_2267_cov_1.278855"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="ACZPE2_03215"
     CDS             <1..343
                     /locus_tag="ACZPE2_03215"
                     /inference="COORDINATES: protein motif:HMM:NF040073.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6363 domain-containing protein"
                     /protein_id="HIR1318890.1"
                     /translation="PVEKALADGCDHVFLILTKPIDFVKKPEMFKSAYTKVLKNYPAV
                     IKKLNIRHETYNNEIKKALELSKEGKVTIIAPDDGYSVTAFTRNLRELNHLYNEGYIK
                     AGEILKNKGYL"
     gene            474..>2267
                     /gene="glgP"
                     /locus_tag="ACZPE2_03220"
     CDS             474..>2267
                     /gene="glgP"
                     /locus_tag="ACZPE2_03220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIR1318891.1"
                     /translation="MNYSLSKKKAKELVEAKLSHFMGVSPEEATDEQYYKAIALIVRD
                     MMSQGRTEFSEKAAKAGTKKVYYLCMEFLMGRSLKNNLYNLNLTDVFASVLADYGVKL
                     ENLYECEPDAGLGNGGLGRLAACYLDALATDGYPARGYSILYECGIFKQKLVDGWQTE
                     LPDFWLPGGEVWLVPHEESALEVRFEGNVNESWDGGFHHVDIENYKPVQAVPYDMYVA
                     GKDGKGISVLRLWAAKAPALDMTLFNQGDYMRAMEQNAMAEVISKVLYPADNHPEGKS
                     LRLRQQYFLVSASIQDIVHRHLRVFGTMDNFPEKVAIHINDTHPTLAIPELMRILLDE
                     CGYGWDDAWKIVTKSVAYTNHTVMKEALECWPEDIYSRLLPRIYQITKEIDNRLRSFV
                     WQATNNADQVERMAVVSNGVIRMANLCVASSHSVNGVSALHSEILKETVFHDFYVLTP
                     DKFKNVTNGIAHRRWLCQSNPELTALLTELIGGGFVYDANELLKLREFKDDEKVLEKL
                     REIKTHNKEHFAALVKKNTGIELDPTSIFDVQVKRLHEYKRQHLNALNILADYLMIKA
                     NPSIDYTPHTYIFGAKAAAGYYMAKKIISFIY"
BASE COUNT          664 a          526 c          510 g          567 t
ORIGIN      
        1 ccccgttgaa aaggcacttg cggacggctg cgaccacgta tttctcatat tgacaaagcc
       61 gatagatttc gtcaaaaagc ccgaaatgtt caaaagcgca tacacaaagg tattgaaaaa
      121 ttatcccgcc gttatcaaaa agctgaatat acgccacgaa acatataata acgaaatcaa
      181 aaaagcgctt gaattatcaa aggagggtaa ggttacgatt atagctccgg atgacggata
      241 ttccgtcacc gcatttacaa gaaatctacg agaattaaat catctttaca acgagggcta
      301 tataaaggcg ggagaaatcc ttaaaaataa gggttatctg tgattttttg tctatatcgc
      361 tgaaataatc gttacttatc ttattactgt tgaaatttgc ttaatattag tatagtatag
      421 acaaagagaa gtttaaaaaa cgcatttttt ctcaatatat ttggaggttt tgtatgaatt
      481 attcactttc caagaagaaa gcaaaagagc tcgtcgaagc aaaactttct cactttatgg
      541 gagtttctcc cgaggaggct accgacgagc aatactataa ggctattgct cttatcgtcc
      601 gcgacatgat gagtcagggt agaaccgaat tttcggaaaa ggccgcaaag gcgggaacga
      661 aaaaggttta ctatctctgt atggaattcc tcatgggacg ttctcttaaa aacaatcttt
      721 acaatttaaa tctcacagac gttttcgcga gtgttctcgc cgactacgga gttaagcttg
      781 aaaaccttta tgagtgcgaa ccggacgcag gtctcggcaa cggcggcctc ggcagacttg
      841 ccgcctgcta tcttgacgcg ctcgcaaccg acggataccc cgcaagagga tattccatac
      901 tttatgaatg cggaattttc aagcagaagc ttgttgacgg ctggcagacc gagcttccgg
      961 acttttggct tccgggcggc gaggtttggc ttgtccccca cgaagagagc gcacttgagg
     1021 ttcgctttga gggcaacgta aacgaatcgt gggacggcgg attccaccat gtagacattg
     1081 agaattacaa acccgtacag gcggttccgt atgatatgta tgttgcgggt aaagacggaa
     1141 agggaatttc cgtgcttcgt ctttgggccg ctaaagcacc ggctcttgat atgaccctct
     1201 ttaatcaagg cgactatatg cgtgctatgg agcaaaacgc catggccgaa gtcatttcga
     1261 aggttctcta ccctgccgac aaccaccccg agggaaagag tcttcgtctt cgccagcagt
     1321 attttcttgt atcggcgtca attcaggata tagttcacag acatctgcgc gtattcggca
     1381 caatggataa cttccccgaa aaggttgcca tccacataaa cgacacgcac ccgacgctcg
     1441 caatacccga acttatgaga atactcctcg acgaatgcgg ctacggctgg gacgatgcat
     1501 ggaagatagt cacaaaatcc gtggcttaca ccaaccacac cgttatgaaa gaggctctcg
     1561 aatgctggcc ggaggatatt tacagccgcc tgcttccgag aatttatcag ataacaaaag
     1621 agatagacaa tcgtctgcgc tcctttgtat ggcaggcaac aaacaatgcc gatcaggttg
     1681 agagaatggc tgttgtttca aacggcgtta tacgaatggc taatctctgt gttgcatctt
     1741 cgcacagcgt aaacggcgtt tcggcgctcc acagcgaaat tcttaaggaa accgtattcc
     1801 acgatttcta cgttctcacg cccgacaaat tcaaaaatgt cacaaacggt attgcccaca
     1861 gacgttggct ctgccaatca aaccccgagc ttaccgcact tctcaccgag cttatcggcg
     1921 gcggctttgt ttatgacgca aacgagcttt taaagctccg tgaattcaag gacgatgaaa
     1981 aggttctcga aaaactgcgt gaaatcaaga cgcacaacaa ggagcatttt gccgcactcg
     2041 ttaagaaaaa taccggcata gagcttgatc cgacctcaat ttttgacgtg caggtaaaga
     2101 gacttcacga gtacaagcgt cagcatctca acgcgctcaa tattcttgcc gattacctta
     2161 tgattaaggc aaatccgagt attgattaca ctccgcacac ttacatcttt ggcgcaaagg
     2221 cagccgcagg ctactatatg gcgaagaaaa taatcagctt tatatat
//
LOCUS       DBMDVO010000166         2260 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22862_length_2260_cov_1.017138, whole genome shotgun sequence.
ACCESSION   DBMDVO010000166 DBMDVO010000000
VERSION     DBMDVO010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22862_length_2260_cov_1.017138"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="ACZPE2_03225"
     CDS             complement(<1..541)
                     /locus_tag="ACZPE2_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318892.1"
                     /translation="MNDDNVITENTVPQKKGGKKKQKKKPMGKGSKFILFISIIVLIV
                     SGTFCVKYGVDIIKSKKEAAEIASLANPQFDVPEYDYGDTVFPDGIQEKYKKAYSVNP
                     DLVGWIKIPGTTVNHPVVHGSDNNYYLRRSFYGSYERRGTPFVDYRNTITDGEFDTNT
                     IIYGHNYLDSTMFSDVEKYK"
     gene            complement(538..>1028)
                     /locus_tag="ACZPE2_03230"
     CDS             complement(538..>1028)
                     /locus_tag="ACZPE2_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014517.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR1318893.1"
                     /translation="KEKIMSIEKQTVELLKSKKLKLATAESCTGGLISKRITDVSGSS
                     EVFEGGVVCYSNRFKENVLGVSPETLKKYGAVSRDTAREMVKGVLSLTKADIAVAVTG
                     IAGPSSDDTNKPVGLVYIAVSDGKSTIVKKLLNNFTGDVREQNRSISADTALEMIMEA
                     IR"
     assembly_gap    1029..1038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1039..2187)
                     /gene="thiI"
                     /locus_tag="ACZPE2_03235"
     CDS             complement(<1039..2187)
                     /gene="thiI"
                     /locus_tag="ACZPE2_03235"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1318894.1"
                     /translation="MKEIILIKNGELALKGLNRSSFESTLAKNIKRRLQSLGKFKVIR
                     SQSTITVEPFDESFDMEEAEDQISRIFGIAGYERAAAVEKDMDVILKTTPVYLSEALK
                     NASTFKVEAKRADKTFPLKSPEICCDVGEAVLNAFPHIRVDVKNPDLTIVIEIRENAA
                     YIHGDQKKGAGGMPVGTGGKAALLISGGIDSPVAGWMMAKRGVVLNAVHFASPPFTSP
                     MSEMKVHDLLAKVAKYSGTIKLITVPFTEIQENIRDKCPEELFTLIMRRYMMRISNIL
                     AEKEGCEAIVTGESVGQVASQTLTAMMSTDSVSRLPVLRPLVGMDKNEIVVISRKIDT
                     FDISIRPFEDCCTVFTPKHPRLHPKPEEIEKAELSLDSDALISRAVEGV"
BASE COUNT          497 a          534 c          523 g          696 t
ORIGIN      
        1 ccttgtattt ttcaacgtcc gagaacatgg tggaatcgag gtaattgtga ccgtaaataa
       61 tggtgtttgt gtcaaattcg ccgtcggtta tcgtgttgcg gtaatcgaca aacggagttc
      121 cgcggcgctc gtagctgccg tagaagctgc gccttaaata gtaattgttg tcggagccgt
      181 gcacaacggg atgatttaca gttgtaccgg gaatttttat ccagccgaca agatcgggat
      241 ttacggaata cgcctttttg tatttttcct gaatgccgtc cggaaaaacc gtgtctccgt
      301 aatcgtactc gggcacgtca aactgcggat ttgcaaggct tgctatttcc gccgcctctt
      361 ttttgctctt tattatatca acaccgtatt tcacacagaa ggttccggag actatcaaaa
      421 caattattga tataaagagt ataaatttgc tgcccttgcc catcggcttc tttttttgtt
      481 tctttttgcc gccttttttc tgcggaacgg tgttttcggt gatgacatta tcgtcgttca
      541 tcgtattgcc tccataatca tttcgagagc ggtatccgcg ctgatgctgc ggttttgctc
      601 cctgacgtcg ccggtaaaat tgtttagaag ctttttaact attgtgcttt taccgtcgga
      661 aacggctata taaacaaggc ccacgggctt atttgtatcg tccgaagacg gtcccgctat
      721 acccgtaacg gcaactgcta tatcggcctt tgtaagagac aaaacgccct ttaccatttc
      781 acgcgcggta tcgcggctta cggcaccgta tttttttagg gtttccgggg aaacgccgag
      841 cacattttct ttgaaacggt ttgaataaca gacaacgccg ccctcgaata cctcggagga
      901 gccggatacg tcggtaatgc gttttgaaat gagtccgccg gtacagctct ctgccgtggc
      961 aagcttaagt tttttacttt tgagaagttc taccgtctgc ttttcaatac tcataatttt
     1021 ttcctttann nnnnnnnnta cgccctcgac cgcacggctt ataagcgcgt cgctgtcaag
     1081 cgataattcg gctttttcga tttcttcggg cttcgggtga agccgcggat gcttcggtgt
     1141 aaataccgta cagcaatcct caaacggtct tatcgaaata tcaaatgtgt ctatctttct
     1201 tgaaataacg actatttcgt tcttatccat acctacaagc gggcgaagca ccggtaagcg
     1261 cgaaacgctg tcggtagaca tcatcgccgt aagagtttgg cttgccacct gacctacgct
     1321 ctctcccgtg acaatagcct cgcaaccctc tttttcggca aggatatttg atatgcgcat
     1381 catataacgt ctcataatga gggtgaaaag ctcctcggga catttatcgc gtatgttttc
     1441 ctgtatctcg gtgaacggca cggttataag tttgattgtg ccgctgtact ttgctacttt
     1501 agcgagaagg tcgtgaacct tcatttcgga catgggcgat gtgaacgggg gagaggcgaa
     1561 atgtacggcg tttaaaacaa ctccgcgttt tgccatcatc cagcctgcaa cggggctgtc
     1621 gattcctccc gagataagaa gtgccgcttt gccgcccgtg ccgacgggca taccgcctgc
     1681 gccctttttc tggtcgccgt gaatgtaagc cgcgttttcg cggatttcta ttactatggt
     1741 taaatcggga tttttaacat cgactctgat gtgcggaaaa gcgtttaaaa ccgcctcgcc
     1801 gacatcacag catatttccg gtgattttaa ggggaaggtc ttgtctgcgc gttttgcctc
     1861 caccttaaag gttgaagcgt ttttaagagc ctcgcttaaa tacacgggag tcgttttgag
     1921 gattacatcc atatcctttt cgactgcggc ggcgcgctca tagcctgcaa tgccgaatat
     1981 gcgtgaaatt tgatcctctg cctcttccat atcaaagctt tcgtcaaaag gctctacggt
     2041 tattgttgac tgtgagcgga ttaccttgaa ttttccgagc gactgaagac gccttttgat
     2101 attttttgcg agcgttgact caaatgacga gcggttaagg cccttaagcg caagctctcc
     2161 gttttttata agtattattt ctttcatatt gctgtttctt tatcctttgt gttttctgag
     2221 tttttcgaca gcgattttta tgacatcggc acagcggtca
//
LOCUS       DBMDVO010000167         2259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_22874_length_2259_cov_1.296571, whole genome shotgun sequence.
ACCESSION   DBMDVO010000167 DBMDVO010000000
VERSION     DBMDVO010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_2259_cov_1.296571"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..694
                     /locus_tag="ACZPE2_03240"
     CDS             20..694
                     /locus_tag="ACZPE2_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1318895.1"
                     /translation="MDRKELIEQNLGLVHACANRFRGRGIEYEELYSAGCLGLVKAAD
                     GFREELGFAFSTYAVPAVLGEIRRLFRDGGAVKVSRSLKERSREAVKLSEKLTKENGR
                     EPTVTELSKILGIDCFAVSELLNISLPPVSLTASDDDNERQTDVPVPSGEDAIQDKLA
                     LEEVLKALPKKDQTLIRLRYFKGFTQSATAEKLGMSQVQVSRKERAVLAIMRKKLTDD
                     YSQSAT"
     gene            complement(679..1290)
                     /locus_tag="ACZPE2_03245"
     CDS             complement(679..1290)
                     /locus_tag="ACZPE2_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HIR1318896.1"
                     /translation="MADIVYTFEGSVYLNITNSCPCKCKFCIRNNSDSVGDADTLWFS
                     GHNPSFDEIKKAIDAYDFTGYNGEVIFCGYGEPTFAYDNLIKTCKYIHEKLGLKVRLN
                     TNGLSDLLNKKPTAKELCENTDKISISLNEYSSEKYCELCAPAFGEKSFDAIIKFAKE
                     CKQYGQDLRFSVVDVIPKEDIEKCRELADSLGIPLRVRAYVAD"
     gene            complement(1305..1844)
                     /locus_tag="ACZPE2_03250"
     CDS             complement(1305..1844)
                     /locus_tag="ACZPE2_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HIR1318897.1"
                     /translation="MNTKSKSMTALLNLVKAAVFAALIAVMTAYIKFNTGINDGYLHF
                     GDSMIYLAACILPLPYAMAAAAIGGFCADILAGAAAWAPFTAVIKALNALPFALVYTC
                     HATKSPNKILNKTTAFMPLVSGLITIFGYLLAEGLLYSFPTALTSVPFSIIQAVGSAI
                     IYYIAAPALDKVKFKTKLS"
     gene            complement(1933..>2259)
                     /locus_tag="ACZPE2_03255"
     CDS             complement(1933..>2259)
                     /locus_tag="ACZPE2_03255"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1318898.1"
                     /translation="AKAASSLLNDTLSTVIVTGIKKDDTVLNVVADKSGFKTVSSKKY
                     GGSFSGTGDLFAAAVAGLTVKGEAPLCATEKAVEFLEGAIRDSYEENTDRNFGVNFEK
                     YLGKLM"
BASE COUNT          620 a          495 c          555 g          589 t
ORIGIN      
        1 cgataaaggg taaagagcaa tggaccgaaa agagctgatt gagcaaaatc tcggcttggt
       61 acacgcctgt gcaaacaggt tccgcggcag aggaatagaa tatgaggagc tttattccgc
      121 agggtgtctc ggacttgtca aggcggcgga cggctttcgc gaggagctcg gctttgcttt
      181 ttcaacgtat gccgtacctg cggttttggg tgaaatcaga agacttttca gagacggcgg
      241 agcggtaaag gtcagccgct ccttaaagga acgctcgcgc gaagccgtta agctcagcga
      301 aaagctgaca aaagaaaacg gcagagagcc taccgtaaca gaattatcaa aaattctggg
      361 gatagactgc tttgccgtat cggaactttt aaatatttcc ttgccgcccg tgtcgctcac
      421 tgcatcggac gacgacaatg aacgtcaaac cgatgttccc gtgccctcgg gcgaagatgc
      481 cattcaggat aaattggctc tcgaagaggt tttaaaagct ttgccaaaaa aagaccaaac
      541 ccttataagg ctcagatatt tcaaggggtt tacgcaaagt gcgacagccg aaaaactcgg
      601 aatgtcacag gtgcaggttt caagaaagga gagagccgtg cttgcgatta tgcgaaaaaa
      661 gctgacggac gattattctc agtcggcaac ataggctctg actctaagcg ggataccgag
      721 actgtcggca agctctctgc atttttcaat atcctctttg gggattacat cgaccaccga
      781 aaaacgcaga tcctgaccgt actgtttaca ctcttttgca aatttgatga tggcgtcaaa
      841 cgatttttca ccgaatgccg gagcacacag ctcacagtat ttttcgctgc tgtattcgtt
      901 aaggcttatc gagattttat cggtattttc acataattcc ttggctgtcg gctttttgtt
      961 gagcaggtcg gaaaggccgt tcgtattaag acgaaccttt aagccgagct tttcgtgaat
     1021 gtatttgcac gtctttatga gattgtcata tgcaaaagtc ggttcaccgt agccgcagaa
     1081 tataacctcg ccgttgtagc cggtgaaatc gtatgcgtca atagcttttt taatttcatc
     1141 aaacgagggg ttgtgacccg agaaccaaag cgtgtccgcg tcacctaccg aatccgaatt
     1201 attgcggatg cagaatttgc atttgcacgg acaggaattt gtgatgttaa ggtatacgct
     1261 gccctcaaag gtgtaaacta tgtcagccat taaaaagact ccctttagga taattttgtt
     1321 ttgaatttaa ctttatcaag cgccggagcg gcaatatagt agattattgc actgccgact
     1381 gcctgaataa tcgagaacgg aacggaggtt aatgccgtgg ggaacgaata taaaagtccc
     1441 tccgcaagaa gataaccgaa gattgttata agaccggaaa caagaggcat aaacgctgtg
     1501 gtcttattaa gaattttgtt ggggctcttc gttgcgtggc aggtataaac aagtgcaaac
     1561 ggcagagcgt taagagcctt aattactgcc gtaaacggag cccaagcggc agcacccgct
     1621 agtatatcgg cacaaaaacc gcctattgcc gcagccgcca ttgcgtacgg aagcggaaga
     1681 atacatgcgg caaggtaaat catactgtcg ccgaagtgga gataaccgtc gtttataccg
     1741 gtgttaaatt tgatgtatgc cgtcattacg gcaataagag cggcaaaaac cgcagcttta
     1801 acaaggttta aaagcgctgt cattgatttt gattttgtgt tcatagtggt caccatattc
     1861 tttcgtagtg aaatatccga gaagctcgcg gtgccggatt gcagggcgca atacggctct
     1921 ttgtgagatg ctttacataa gcttccccaa atatttttca aaatttaccc cgaaattacg
     1981 atccgtgttt tcttcgtaag aatcccttat ggcgccttca aggaattcta cggctttttc
     2041 ggtggcacat agcggagctt cgccttttac ggtaagtccc gcaacagccg cggcaaacag
     2101 atcacccgta ccggaaaagc ttccgccgta ttttttggat gaaacagttt taaaaccgct
     2161 tttatcggcg acaacgttta aaacggtgtc gtcttttttt atgccggtaa caatgaccgt
     2221 gctcagagta tcgtttaaaa gggacgatgc ggcttttgc
//
LOCUS       DBMDVO010000168         2243 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23087_length_2243_cov_1.503268, whole genome shotgun sequence.
ACCESSION   DBMDVO010000168 DBMDVO010000000
VERSION     DBMDVO010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23087_length_2243_cov_1.503268"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(140..1450)
                     /locus_tag="ACZPE2_03260"
     CDS             complement(140..1450)
                     /locus_tag="ACZPE2_03260"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIR1318899.1"
                     /translation="MLGEVTTVYVFLIELGSLLLYCYRVFGVRRRIPSTLLMGIACFA
                     VYYAVNKLADNNVAVNIIFGFLVNYVILKLGFKANVKSAVFHSVLLAGVLTATEFIGI
                     LLISGFFGINIADYRSNDVLYAMAAVIAKTLYLISCLVISNFTSREKQHIDHGHSWYL
                     LISPFSSVYIIVLIAKLSLLVDISGTLAYACIGGSVLLFISDIMVFAVYENMQRKSEE
                     IMKVNMERQREDINRDYYAVLDKQNENMHIMVHDIKNHLGVIESIADNDKVTAYIGEL
                     CSNIGKYYAVSQLTGNKMLDIIIGKYTVLCSKNGLDFNAKALTSNLSFVHDSDISAMV
                     GNLLDNAFEAAKQAENGKIEFSLYTIDGKSCVVSVINTALTAPKCENENLVSAKPDAQ
                     SHGYGIKSIKKTAEKYGGSYSWFYDDKSREFHAMVMLPISSESC"
     gene            complement(1454..2167)
                     /locus_tag="ACZPE2_03265"
     CDS             complement(1454..2167)
                     /locus_tag="ACZPE2_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1318900.1"
                     /translation="MNIAICDDEQLYADEIKKHVECYFTRREVPHKIYVFTDGQAAAR
                     SEVKFDIAFLDIEIGSVSGLDVGKAMRKNNPNIVFIVVTAFSKYLDDALDLKVLRFLE
                     KPINSERLYAGIDRAIDTLNDTTVGFYLRDGDMDYKKILLCDLIMIETDRRRTKVYTD
                     AEVFRTKKTLSEWQRELTEPFFMMPHNSFIINMNKIVKFSRTEIELEGGKKHYTVSVA
                     PKRQAETKRILMSYFESRR"
BASE COUNT          629 a          533 c          427 g          654 t
ORIGIN      
        1 ataccaataa cgttgtttta tgtcaaaata aacagcgtca agtattgcgt cattaaaaca
       61 aaaaactccg accggcaccc gagtgcctat cggagtttta cagtcatttc agtcaaaaca
      121 atatgtaaag ctgattactt taacagcttt cagacgatat cgggagcata accatagcgt
      181 gaaattccct gcttttatca tcatagaacc acgaatagct gccgccgtat ttctcagctg
      241 tctttttaat actttttatt ccgtatccgt gagactgtgc gtccggcttg gcgctcacaa
      301 gattttcgtt ttcgcatttt ggtgctgtca aggctgtatt tatgacgctt acaacgcagc
      361 tcttaccgtc aatggtataa agagaaaact caattttacc gttttctgct tgcttcgccg
      421 cctcaaaagc gttgtcaagc agattgccca ccattgccga aatatcgctg tcatgcacaa
      481 aggacagatt cgaggttaaa gccttggcat taaaatcgag gccgttttta ctgcaaagaa
      541 ccgtatattt gcctatgata atatcaagca tcttatttcc ggtaagctgc gataccgcgt
      601 agtatttacc gatattactg caaagctcac ctatataagc cgtaaccttg tcattatccg
      661 ctatgctctc tattacgccg agatgattct ttatatcgtg aaccataata tgcatattct
      721 cattttgctt gtcaagcacg gcgtaataat ctctgtttat atcctctctc tggcgttcca
      781 tattgacctt cattatttct tcggatttgc gctgcatatt ttcgtatacc gcaaatacca
      841 ttatatcgga gatgaagagc aaaacactgc cgcctataca ggcataagca agagtgccgc
      901 ttatgtcaac gagcagtgac aatttcgcta taagaactat gatatacaca cttgaaaaag
      961 gagaaatcaa cagataccag gaatggccgt ggtctatatg ctgtttttca cgcgaggtga
     1021 aattggaaat cacaaggcag gaaataaggt acaatgtttt ggcgattacc gccgccatgg
     1081 catacagcac atcgtttgac ctgtaatcgg ctatatttat gccgaagaag cccgaaatca
     1141 acagtatacc tataaattcc gtcgccgtaa gtacgccggc aagcaagacg ctgtgaaaaa
     1201 ccgccgactt gacatttgcc ttgaagccga gcttcaatat cacgtaattt accaagaagc
     1261 caaagataat atttactgcc acattgttat cggcaagctt atttacggca taataaacgg
     1321 caaaacaggc tatgcccata agcagcgtcg agggtatcct acgtcttact ccgaacactc
     1381 ggtagcaata caaaagaaga gaaccgagct ctatcagaaa aacatatacg gttgtgacct
     1441 cgccgagcat agttcatcgc ctgctttcaa aataactcat aagtatacgc tttgtctccg
     1501 cctgacgctt gggtgccacc gacacggtat aatgcttttt gccgccctca agttcgattt
     1561 ccgttcttga gaatttcact attttattca tatttatgat aaagctgtta tgcggcatca
     1621 taaagaacgg ctcggtcagc tctctttgcc actcggaaag cgttttcttt gtgcggaaaa
     1681 cttccgcatc ggtatagacc tttgttctgc gcctgtccgt ttctatcata ataaggtcgc
     1741 aaagcagtat ctttttatag tccatatcgc cgtcacgcag gtaaaagcct accgtggtat
     1801 cgtttaaagt gtctatggca cggtctatac cggcatataa cctttcgctg tttatcggct
     1861 tttcgagaaa tcgcagaacc tttaaatcca acgcgtcgtc gaggtatttc gaaaacgccg
     1921 taacaactat aaaaacgata ttaggattgt tttttcgcat cgcctttccc acatcgagcc
     1981 cgctgacaga ccctatctct atatcaagaa aggcaatgtc gaatttcact tcgctccgag
     2041 cagccgcttg cccgtcggtg aacacatata ttttgtgcgg aacctctctg cgcgtaaaat
     2101 aacattcgac gtgctttttt atctcgtccg cataaagctg ctcgtcgtcg catattgcaa
     2161 tattcattta gatccccctc cgaataaaaa ataaggcata tcgctttgcc tttaccgata
     2221 ttgtattata ttacaaaacc cga
//
LOCUS       DBMDVO010000169         2240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23146_length_2240_cov_1.011688, whole genome shotgun sequence.
ACCESSION   DBMDVO010000169 DBMDVO010000000
VERSION     DBMDVO010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23146_length_2240_cov_1.011688"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="ACZPE2_03270"
     CDS             complement(<1..433)
                     /locus_tag="ACZPE2_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318901.1"
                     /translation="MRDNIKYMLSNWLKWDRKSLVYFLIRIPALVFQPIVTSYIPKAM
                     IDCINEGATVGRLTLVVGLLSVLVVLTTWLSPFMQELLNGSARIIRMRYAVLAFNKNL
                     NTDYTNIESPGGREKNKRAMEFYRSYYSGSSDFLDSCNQFCV"
     gene            614..856
                     /locus_tag="ACZPE2_03275"
     CDS             614..856
                     /locus_tag="ACZPE2_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318902.1"
                     /translation="MKIKDAVAQRIIDLCKEKNIAINALGNISGVNPSTIYSILNTKS
                     QNPGVVTLQKICDGLEISIRDFFDVELFDDIEQEII"
     gene            complement(888..1751)
                     /locus_tag="ACZPE2_03280"
     CDS             complement(888..1751)
                     /locus_tag="ACZPE2_03280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1318903.1"
                     /translation="MGRKIEFFIDNDNGNVKIKDYLKRRGVSHRMLCVLKRTENGILK
                     NGVGARSIDILNSGDTLTLNLPSDEGTASAEPADIPLDIIYEDDDILVINKKPDIAMH
                     ESHNHRCDALSNAVAFHLMKEGKECVFRSVGRLDKGTSGIVVCALNKFSAAKLSGKIY
                     KEYLAVADGEFYGDGVIDKPIIRPDPMKTLRDVGVGGERAVTHWKSLKVKDGCTLLRI
                     HLETGRTHQIRVHFKSLGAPLVGDDMYGSTDKRLGHQALHCCFVRFTHPVTEKIMEFT
                     APMPDDMNKLT"
     gene            1839..2180
                     /locus_tag="ACZPE2_03285"
     CDS             1839..2180
                     /locus_tag="ACZPE2_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318904.1"
                     /translation="MQLVIMIIHDSDHMNSILVKMMEAGIKGGSLIDCEGALQAIGRA
                     SSDPPPVFGSLREYLNPESAAKNKLLLCAMEDEKVAVAKQIANEVTGGLSKPNTGVMF
                     CLPLLSHEGLK"
BASE COUNT          639 a          535 c          466 g          600 t
ORIGIN      
        1 atacgcaaaa ttgattgcac gaatcgagaa aatccgacga acccgaataa tagctgcggt
       61 aaaactccat tgctctctta ttcttttctc tgccgcccgg gctttcgata tttgtataat
      121 cggtgttcaa attcttattg aaagcaagca ccgcatagcg catacgaata atacgcgcgc
      181 tgccgtttaa gagctcctgc ataaacggcg aaagccaagt agtcagcaca acgagcacgc
      241 tcaaaagccc cacgacaagc gtaagtctgc ctaccgttgc gccctcgttt atgcaatcga
      301 tcatagcctt tggtatataa gaagtgacaa tcggctgaaa cacaagcgcc ggtatgcgta
      361 taagaaaata cacaaggctt ttcctatccc atttaagcca attcgacagc atatatttta
      421 tgttatctct cattatttta tcctccgtaa gcacctgtgc aaaagcccct cacgcgacat
      481 accgcgaaat cggcataatt gaatattaca ttagccgcac ataaaagtca ataaaaaaat
      541 ttgggatata tcccacacga tatatcccaa attcctatac gatttaacta tcactgacaa
      601 aaaggcggta gttatgaaaa ttaaagatgc tgttgctcaa agaataatcg atttatgtaa
      661 ggaaaagaac attgccataa atgcgctcgg caacataagc ggcgtaaatc cgtccacgat
      721 ttacagcata ctgaacacaa aaagtcaaaa tcccggcgtt gtaactctgc aaaaaatatg
      781 cgacggcttg gaaatatcaa tacgggattt ttttgacgtc gagctttttg acgacattga
      841 gcaggaaata atataaaaac gaacagactg catctgtcgg cggtccgtta cgttagctta
      901 ttcatatcat ccggcatagg tgcggtaaat tccataattt tctcggtaac ggggtgtgta
      961 aagcggacaa aacagcaatg cagagcctga tggccgagtc ttttatccgt actgccgtac
     1021 atatcgtcgc ccacaagcgg cgctccgagg cttttaaaat gaacacgtat ctgatgagtt
     1081 ctgcccgttt cgagatgtat tctcaaaagc gtacagccgt cctttacctt taacgatttc
     1141 caatgtgtaa cggctctttc gccgcccacg ccgacatcgc gcaaggtctt catggggtcg
     1201 ggtcttataa taggcttatc tataacgccg tcgccgtaaa attcgccgtc ggcaacagct
     1261 aaatactcct tatatatttt accgctcagc tttgccgccg aaaatttatt gagagcgcac
     1321 accactatgc ccgacgttcc cttatcaagt ctgccgacag agcggaatac gcactccttt
     1381 ccctctttca taagatgaaa tgccaccgca tttgacagcg cgtcgcaacg gtgattgtgc
     1441 gattcgtgca tggcaatatc gggctttttg tttatcacga gtatatcgtc gtcctcataa
     1501 attatgtcga gcggtatatc ggcaggctca gcggaggcgg tgccttcgtc agacggcaaa
     1561 ttcaaagtca gcgtatcacc cgaatttaaa atgtcgatgc ttctcgcccc tactccgttt
     1621 ttcaatatgc cgttttcggt gcgctttaaa acgcagagca ttctgtgcga cacgcccctg
     1681 cgctttaaat aatcttttat tttgacattg ccgttgtcat tgtcaataaa aaattcgatt
     1741 ttgcgaccca taattccacc atcgttaaaa ttctgtttta ttgcattata tcatataaag
     1801 gtgtaaaata atatcgaaat ttaaaaggga ggaatactat gcagctcgta ataatgataa
     1861 ttcacgacag cgaccacatg aattccattc tcgtaaaaat gatggaggca ggtatcaagg
     1921 gcggctcgct tatagattgc gagggtgctc ttcaggcaat agggcgcgcc tcctcggatc
     1981 cgccgcctgt tttcggctcc ctgcgcgaat atttaaaccc cgaaagtgcc gcaaaaaaca
     2041 agctacttct ttgcgctatg gaggacgaga aggtcgcagt cgcaaaacag attgcgaacg
     2101 aagtcaccgg cgggctctca aagcccaata ccggcgttat gttttgcctg ccgctgctgt
     2161 cgcacgaggg attaaaataa tattgaatct gatacggagg aaaaatttat gcagcttttg
     2221 ttttttgtac tgaaccgctc
//
LOCUS       DBMDVO010000170         2222 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23402_length_2222_cov_1.064592, whole genome shotgun sequence.
ACCESSION   DBMDVO010000170 DBMDVO010000000
VERSION     DBMDVO010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23402_length_2222_cov_1.064592"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            7..>1296
                     /locus_tag="ACZPE2_03290"
     CDS             7..>1296
                     /locus_tag="ACZPE2_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1318905.1"
                     /translation="MAGYIKLKKSNCKNCYKCIRNCPVKSISFSANQAQIVEDECILC
                     GMCFVACPQNAKIIRDDVYKAKELLSGDSEVYVSLAPSFIANYDVSFTAMKKALMSLG
                     FAGVEETAVGAAIVKDEYDRIVDGEKQDVVISTCCHTVNLLVQKHFPDVIPYLAKVVS
                     PMQAHCTKLKKEHPGAKTVFIGPCISKKAEAEEYPGTVDCVLTFEELSGWLAETDTVL
                     AQEPDDDGVEKGKTRFFPTSGGILKTMLCDNDDYTYISIDGMSSCIHAVKDIEKGNIH
                     HCFIEMSACPGSCIGGPAMEKNHRAPVRDYITVRRFAEDAGDNDFKYDALSENELSKN
                     LIYLASPHNMAGSRAIDEILRKMGKQKPEDELNCGSCGYNTCREKAQAVFEGKANLEM
                     CLPFLKDKAEKFSDNILNNTPNGILILNEDMVVQQINA"
     assembly_gap    1297..1306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1307..1698
                     /locus_tag="ACZPE2_03295"
     CDS             <1307..1698
                     /locus_tag="ACZPE2_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1318906.1"
                     /translation="EIMNIRYASDVVGEPVVRILEPFDFIDVLSNGRDIHDKRVYLAE
                     YKKYIEETIVYDHVYHILMCIMRDVTAEETEKEKKAELSRQTIEITDKVVEKQMRIVQ
                     EIASLLGETTAETKIALTKLKESLKDE"
     gene            1691..>2222
                     /locus_tag="ACZPE2_03300"
     CDS             1691..>2222
                     /locus_tag="ACZPE2_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1318907.1"
                     /translation="MNNLCTDIGHMSLTKYGEELCGDMVEVIEQGDDDYVVVLADGLG
                     SGVKANILSTLTSKIISTMIANNMPLEMCVDTIAKTLPICSVRQVAYSTFTIVRVIDN
                     KEAEIAQYDNPHVILLRDGKNLVYPESAVEIDGKMIYKSKISLKLNDTLIFTSDGAVY
                     AGIGENMNFGWQRDQII"
BASE COUNT          654 a          433 c          554 g          571 t
ORIGIN      
        1 tataatatgg cggggtacat aaagcttaaa aaatcaaact gcaaaaactg ctataaatgt
       61 atcaggaatt gtcctgtcaa atctatcagt ttttcggcca atcaggcgca gatcgttgag
      121 gatgagtgta ttctttgcgg tatgtgcttt gtcgcgtgtc cgcaaaatgc caaaatcata
      181 cgcgacgacg tctataaggc aaaggaatta ttatccggtg acagcgaggt ctatgttagc
      241 cttgcgccgt cgtttattgc caattacgac gtgtcgttta ccgcgatgaa aaaagcactt
      301 atgtcgctcg gctttgccgg cgttgaagaa acggcggtcg gagccgcaat agtaaaggac
      361 gagtacgaca gaatagttga cggtgaaaag caggatgttg tcatttcgac ctgctgccat
      421 accgtaaatc tcttggtaca aaagcatttc cccgatgtaa taccctatct tgccaaagtg
      481 gtatccccaa tgcaggcgca ttgtacgaaa cttaaaaaag agcatccggg ggctaaaaca
      541 gtatttatag gtccgtgcat atcaaaaaag gcggaggccg aggagtatcc cggcacggtt
      601 gactgtgtgc tgacatttga agagctttcc ggttggcttg ccgaaaccga tactgttttg
      661 gcgcaggagc ctgatgatga cggagttgaa aagggaaaaa cgaggttttt cccgacctcc
      721 ggcggaattc tcaaaacaat gctttgtgat aacgatgatt acacgtatat ttcgattgac
      781 ggtatgtcgt cttgcatcca tgccgtaaag gatattgaaa aaggtaatat acaccattgc
      841 tttatagaaa tgtctgcgtg tccgggaagc tgtataggcg gtccggctat ggagaaaaat
      901 caccgcgctc ctgtgcgtga ttatataacc gtgcgtcgct ttgccgaaga cgcgggcgac
      961 aatgatttta aatatgacgc cttgagcgaa aacgagcttt caaaaaatct tatttatctt
     1021 gcttctccgc acaatatggc aggtagcaga gcgatagatg agatacttcg caaaatgggt
     1081 aagcaaaagc cggaggatga gcttaactgc ggttcgtgcg gctataatac ctgtcgtgaa
     1141 aaggctcagg cggtttttga gggcaaggca aatctcgaga tgtgccttcc tttccttaag
     1201 gataaagcag aaaaattttc cgacaatata ctcaacaata ctcccaacgg catactcatt
     1261 ttaaatgagg atatggtggt tcagcagata aatgccnnnn nnnnnnggga gattatgaac
     1321 attcgttatg cttcggatgt tgtgggtgag ccggttgtgc gtattttgga gccttttgat
     1381 tttatagatg ttctttcaaa cggcagggat attcacgata aacgcgtata cctcgccgaa
     1441 tataaaaaat acattgaaga aacaatagta tacgaccatg tctaccatat acttatgtgc
     1501 attatgcgcg acgttaccgc cgaggagaca gaaaaagaga aaaaggcgga gcttagccgt
     1561 caaacgatag aaataacgga taaggtcgtc gaaaagcaaa tgcgcatagt tcaggagata
     1621 gcttctctgc ttggcgagac taccgccgag acaaagatag ccctcactaa gcttaaggag
     1681 tcgttgaagg atgaataatc tttgcacgga cataggccat atgagtctta ccaagtacgg
     1741 cgaggagctg tgcggcgata tggtggaggt tatagagcag ggcgatgacg attatgttgt
     1801 tgtgcttgcg gacgggctcg gctccggtgt aaaagcaaat attctctcta ctttgacttc
     1861 gaaaataata tccaccatga ttgcaaataa tatgccgctt gaaatgtgtg ttgatacaat
     1921 agcaaaaaca ctgcctatat gttcggtacg tcaggtggca tacagcactt ttacaatagt
     1981 gcgtgtaata gacaacaaag aggctgaaat agctcaatac gacaatccgc atgtcattct
     2041 gctgcgtgac ggaaaaaatc ttgtgtatcc cgagagcgca gtggaaatcg acggtaagat
     2101 gatatataaa tcgaaaatat cgttaaagct taacgatacg ctcattttta cgagtgacgg
     2161 agcggtttat gccggtattg gtgaaaatat gaatttcggt tggcagcgcg accagataat
     2221 cg
//
LOCUS       DBMDVO010000171         2220 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23428_length_2220_cov_1.292591, whole genome shotgun sequence.
ACCESSION   DBMDVO010000171 DBMDVO010000000
VERSION     DBMDVO010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23428_length_2220_cov_1.292591"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="ACZPE2_03305"
     CDS             <1..433
                     /locus_tag="ACZPE2_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005212506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1318908.1"
                     /translation="KRSGASLVYAMFSAVFAALTSIFGKIGIEDVESNLGTAIRTVVV
                     LLMAWILVFAEKKQSEIKNIDKKSWLFLALSGFTTGGSWLCYYGALKNGPASIIAPID
                     KLSIVVTVAFSYIILKEKLSKKAFLGLVLIVAGTLSLLIKY"
     gene            451..>1438
                     /locus_tag="ACZPE2_03310"
     CDS             451..>1438
                     /locus_tag="ACZPE2_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1318909.1"
                     /translation="MYGLLGEKLGHSFSPQIHALLGDYEYRLFEKQKNELDDFFKNRN
                     WDGINVTIPYKKAVIPYLDRLSPSAEKIGSVNTIVAEKDGTLTGYNTDYDGFSYLIDV
                     SGISAENKKCLVLGSGGASLTVIAVLKDKKAREIVNISRSGENNYENIDRHFDADIIV
                     NTTPVGMYPNNLKSPLSLDGFKNLSGVLDIVYNPQKTKLILDAEKRNIKALSGLSMLV
                     AQAKRAAELFLNTKIPDSKNDDIYHKLSLEMKNIILIGMPGSGKTTVGKRIAEALNRD
                     FIDTDEMIVKNVGKSIPDIFANGGEKLFRKYEHEAVLAAGKLSGKVISTGGGV"
     assembly_gap    1439..1448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1449..1682
                     /locus_tag="ACZPE2_03315"
     CDS             <1449..1682
                     /locus_tag="ACZPE2_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318910.1"
                     /translation="NFDAPKQNGTIIFLNRSTSYLPTDGRPLSQSNDLNEMLKKRLPL
                     YRKFCDIEADGNDTIENVANSILKELQNENTCN"
     gene            1663..2094
                     /gene="aroQ"
                     /locus_tag="ACZPE2_03320"
     CDS             1663..2094
                     /gene="aroQ"
                     /locus_tag="ACZPE2_03320"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR1318911.1"
                     /translation="MKILVINGPNINMLGIREPAIYGDRNYETLINQINVWGAELHAE
                     VECYQSNHEGDIVDKIQDALGKFDGIVINPAAYTHTSVAILDALKAVSIPACEVHISD
                     VNEREKFRHISYAGMACEAHFIGFGFDGYKKAMEYLCEKYK"
BASE COUNT          692 a          456 c          523 g          539 t
ORIGIN      
        1 caaaaggagt ggagcttcgc ttgtatatgc catgttctcc gctgtttttg cggccttaac
       61 atcgatattc ggaaaaatcg gcatagagga cgttgaatca aatctcggca cggcgataag
      121 aaccgtggtt gttcttctga tggcgtggat tttggtcttt gcggaaaaga agcagagcga
      181 aatcaagaac attgataaaa agagctggct gtttttggcc ctttcggggt tcaccacagg
      241 cggctcatgg ctttgctatt acggagccct gaaaaacggc cccgcaagca tcattgcacc
      301 gatagacaaa ttgagcatcg ttgtaaccgt agccttttca tatataatat taaaagaaaa
      361 gctttcaaaa aaggcttttc tgggacttgt gctgatagtg gcgggtactc tgtcgcttct
      421 tattaaatac tgattgaaac ggagacttat atgtacggac ttttgggtga aaaattaggt
      481 cacagctttt cgccgcagat acatgctctg ctcggcgatt atgaatacag attgtttgag
      541 aagcaaaaga atgagcttga cgactttttt aaaaacagaa attgggatgg cataaatgtt
      601 acaattccgt ataaaaaagc cgtcatcccc tatctcgacc gtctttcgcc ctccgccgag
      661 aaaataggga gcgttaacac gatagttgcc gaaaaggacg gcacgcttac gggatataac
      721 acggattacg acgggttttc atatttaatt gatgtttccg gaatatcggc agaaaacaaa
      781 aaatgtctcg ttttgggttc cggcggcgca tctttaacgg tgatagcggt gctgaaggat
      841 aaaaaagcgc gcgagattgt caatatttca cgctcgggag aaaacaatta cgaaaatatc
      901 gacagacatt ttgacgcgga cataatcgta aacacaacgc ccgtgggaat gtatccgaac
      961 aatctgaaat cgcctttatc gcttgacgga tttaaaaatc tttccggtgt tctggatata
     1021 gtgtataatc cgcaaaagac caagctgata cttgacgccg aaaagcgaaa tatcaaagcg
     1081 ctgagcggtc tttcaatgct tgttgctcag gccaaaagag ctgccgagtt gtttttgaat
     1141 acaaaaatac ccgacagtaa aaatgacgat atatatcata agctctcact tgaaatgaag
     1201 aatattattt taataggtat gcccggttcc ggcaaaacga ctgtcggaaa aagaatcgcc
     1261 gaggccttga accgcgactt tatagacaca gatgaaatga tagtaaagaa cgtcggcaaa
     1321 tccattcccg acatatttgc aaacggcggt gaaaagctct tcagaaaata tgagcacgag
     1381 gcggtgcttg cggcgggcaa gctcagcggc aaggtaattt caacaggcgg cggagtcgnn
     1441 nnnnnnnnaa cttcgacgct ccgaaacaaa acggaacgat aatcttttta aaccgcagca
     1501 cctcttatct gccgacagac ggcaggccgc tgtcgcagag caacgacctt aacgagatgc
     1561 tcaaaaaaag acttccgctt tacagaaaat tctgtgatat tgaggcggac ggaaatgaca
     1621 cgatagaaaa tgttgcgaac agtattttaa aggagctgca aaatgaaaat acttgtaatt
     1681 aacggtccga acatcaatat gctcggcata agagagcctg cgatttacgg cgacagaaat
     1741 tacgagaccc tcataaatca gataaacgtc tggggcgcag agcttcacgc cgaggttgaa
     1801 tgctatcaat cgaaccacga gggcgatata gtagacaaaa tacaggacgc attaggtaaa
     1861 ttcgacggta tagtcataaa tccggcggca tacacacaca caagcgttgc aatactcgac
     1921 gcgctgaaag ccgtttccat accggcttgc gaggtacaca tttcggacgt taacgaacgc
     1981 gaaaaattca gacatatttc atacgccggt atggcgtgcg aggcgcactt tatagggttc
     2041 ggatttgacg gatacaaaaa agctatggaa tatctgtgcg aaaagtacaa gtaaaaaagc
     2101 ataaaataaa cggggctgca aggctgagcg ttcataagga agtattggtt tctcgcataa
     2161 aaatctctta tgcttttgtc aaaaagcctc gcaaggcgac agccttgctt catttcttga
//
LOCUS       DBMDVO010000172         2218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23458_length_2218_cov_1.237600, whole genome shotgun sequence.
ACCESSION   DBMDVO010000172 DBMDVO010000000
VERSION     DBMDVO010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23458_length_2218_cov_1.237600"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="rimM"
                     /locus_tag="ACZPE2_03325"
     CDS             complement(<1..343)
                     /gene="rimM"
                     /locus_tag="ACZPE2_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1318912.1"
                     /translation="MLKQFLEAGKVVGMHGVRGEMRFDVWCDGADFLKKIKTLYLDAD
                     GKRAVGVVSVRPHKNAALLKLDGVLTIEEAEKYRGRVLYFDRKDVHLDSGKAFVEDVI
                     GCAVYDADNTDV"
     gene            complement(423..656)
                     /locus_tag="ACZPE2_03330"
     CDS             complement(423..656)
                     /locus_tag="ACZPE2_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1318913.1"
                     /translation="MKELLEQIAKGLVDEPDSVSVTVDETDDRGVTVYHLHVAENDMG
                     RVIGKQGRIAKAIRTVVKARATKDGTRVQVEID"
     gene            complement(672..917)
                     /gene="rpsP"
                     /locus_tag="ACZPE2_03335"
     CDS             complement(672..917)
                     /gene="rpsP"
                     /locus_tag="ACZPE2_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1318914.1"
                     /translation="MAVKIRLRRVGAKKAPFYRVVVADSRAPRDGRFIEEIGTFDPMK
                     SPAEIKIDAEKAEQWLKNGAQPTETAKSVLKQSGIIK"
     gene            complement(986..>2218)
                     /gene="ffh"
                     /locus_tag="ACZPE2_03340"
     CDS             complement(986..>2218)
                     /gene="ffh"
                     /locus_tag="ACZPE2_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1318915.1"
                     /translation="LALLEADVNYKVAKDFTNKVTERAIGSHVLESLTPAQMVIKIVN
                     EELTELMGGTGSKLAYASHGPTIIMMCGLQGSGKTTHCAKIALKLKTEGHRPLLVACD
                     VYRPAAIKQLQVVGEQAGVTVFEEGQGDPVKIAKDAVSLAKDKGYDYVFIDTAGRLHI
                     DETLMNELKNIKAEVHPHEILLVVDAMTGQDAVTVASAFDDALGIDGMVLTKLDGDTR
                     GGAALSARAVTGKPIKFVGMGEKLTELDYFHPDRMASRILGMGDMLSLIEKAEMALDE
                     KKAAELEKKLRKNKFDLNDLLSQLQQIRKMGSMKDLLGMLPGVGSKIKDMDVDESQFD
                     RVQAIIFSMTYKERSNPDIINPSRKRRIAAGCGMQVEDVNKLLKQYRDMQKLFKQFNS
                     KSARRRFSKMGNFGNYGM"
BASE COUNT          550 a          584 c          454 g          630 t
ORIGIN      
        1 atacatcggt attatccgcg tcatacacag cgcagcctat tacatcctca acgaacgctt
       61 tgccgctgtc aagatgaaca tcctttcggt caaaatacaa cactctgccg cggtatttct
      121 ctgcttcttc gattgtaaga acgccgtcga gctttaacag ggctgcattt ttatgagggc
      181 gcaccgaaac aacgccgact gccctcttac cgtcggcatc aagatacaat gttttgattt
      241 ttttcaaaaa gtccgcgccg tcgcaccata cgtcaaagcg catttcgcct ctgacgccgt
      301 gcataccgac taccttaccg gcctcaagaa attgttttaa catataaatt caccgattgc
      361 cgctttaaaa cggcactaaa taaaatgagt acaagaggaa gcccccctgt actcgtttac
      421 tgtcagtcga tctcaacctg aacccttgtg ccgtcctttg tggctcgagc cttaacaacg
      481 gttctgatag ccttggctat tctgccctgc ttgcctataa ctctgcccat atcgttttcg
      541 gcaacgtgaa ggtgatacac cgtaacgcct ctgtcatcag tctcatctac cgtaacggaa
      601 accgagtccg gctcatcaac gagtccttta gcgatctgtt cgagaagctc tttcaactaa
      661 aagacctcct attatttaat gattccgctc tgctttaata cagatttagc ggtctctgtg
      721 ggctgagcgc cgtttttgag ccactgctct gccttttcag cgtcaatctt aatttcagcc
      781 ggagacttca taggatcaaa agtacctatt tcttcgataa atctgccgtc acgcggagct
      841 cttgaatctg caacaactac acgatagaaa ggagcttttt tagcacctac acgacgaagt
      901 ctgattttta ctgccattgt tttttcctcc aaatatataa ttattaaatt tataaactac
      961 acttataaag tgcgattaac aaaagttaca ttccgtaatt gccgaaattt cccattttac
     1021 tgaagcgacg acgtgcagat tttgaattaa actgtttaaa cagcttctgc atatcgcgat
     1081 actgcttcaa aagcttgttg acatcctcaa cctgcatacc gcaacctgcg gcaattctgc
     1141 gcttacgtga cggatttata atatcggggt tggagcgctc cttatatgtc atcgaaaaga
     1201 tgattgcctg aacgcggtca aactgactct cgtctacgtc catatccttt atcttactgc
     1261 cgacgccggg gagcataccg agcaaatcct tcatagagcc catttttctt atctgctgaa
     1321 gctgagacag aaggtcgttt aaatcaaact tgttttttct gagtttcttt tcaagctcgg
     1381 cggccttctt ttcgtcaaga gccatttccg ccttttcgat aagggaaagc atatcaccca
     1441 taccgagaat tcgcgaagcc atacggtcgg ggtgaaaata gtcaagctcc gtgagcttct
     1501 cgcccatacc gacaaacttt ataggcttac cggtaacagc tctcgcggag agcgccgcgc
     1561 cgcctcttgt atcaccgtcg agttttgtaa gtaccatacc gtcgatgccg agcgcatcgt
     1621 caaatgcact tgccaccgtg acggcgtcct gaccggtcat agcgtcaaca acaagaagaa
     1681 tctcgtgggg atgaacctcg gccttaatgt ttttaagctc gttcatcagc gtttcatcta
     1741 tatgaagacg acctgccgta tctatgaaaa cgtagtcata tcccttatcc ttggcaaggg
     1801 aaactgcatc tttcgctatt ttaacagggt cgccctgacc ctcttcgaac acggtgactc
     1861 cggcctgctc accgactacc tgcagctgct ttatggcagc cgggcggtac acgtcgcacg
     1921 caacaagcag cggtctgtga ccctcggttt taagcttcaa tgctatttta gcacaatgag
     1981 ttgttttacc ggagccctga aggccgcaca tcataatgat tgtgggtccg tgtgaagcgt
     2041 atgcgagctt tgagccggtt ccgcccataa gctcggtcaa ttcctcattg actattttta
     2101 taaccatttg agccggtgta agactctcta aaacgtgtga gcctattgcg cgctcggtaa
     2161 ctttatttgt aaaatccttt gcgaccttat agttaacgtc cgcttcaaga agagcgag
//
LOCUS       DBMDVO010000173         2189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23906_length_2189_cov_1.204502, whole genome shotgun sequence.
ACCESSION   DBMDVO010000173 DBMDVO010000000
VERSION     DBMDVO010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23906_length_2189_cov_1.204502"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="ACZPE2_03345"
     CDS             <1..736
                     /locus_tag="ACZPE2_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1318916.1"
                     /translation="TEIIRSMNLISVFKQEKGVKRFFMMIFSILLMGFGISLFSYSDM
                     GVDPFTSMNMAISGKIGISFGFYQMCINCVILIAIAFLGRHLIGFGTVVNMVGVGYIC
                     EFFTSLYDAHLPVEKSLALRLVLMLFGVIFLSLAASMYFTSNLGVAPYDATGFVLDDR
                     TKIKYKWCRVITDLICTAVGFAFGGPVGIGTVVTAFMMGPVVAFFNKHVSEKLLAAKM
                     PRLVVRVYDLRRFGGGVISSNGKFAA"
     gene            1041..>2189
                     /locus_tag="ACZPE2_03350"
     CDS             1041..>2189
                     /locus_tag="ACZPE2_03350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1318917.1"
                     /translation="MLTDNLCMGCMRELGEEKVCPHCGLHADFVQESPLLPLRTVIND
                     RYIIGKAISTGGDGVTYIAWDRERNVAVAVREFLPLEHVIREFGDRAITVKKDSSLVV
                     HDGIMAFLELWRKIARLRDLSALKPVVDIVTENNTAYSVSEYIESITLRDFLLKSRTG
                     YLGWDQAKTLLMPVLSTLATMHEAGIYHLGISPNTLLLGRDGKLRLTGFMISDARLTN
                     TDFNAELVKGYVPYEQYDPNGKVGAWSDVYAFTAVVYRTLIGSTPDDSSERVVNDKLL
                     IPAKFAEVLPAYLVSAIHNGLQLQPEDRIRTVDDLREQLSGSPSTMATNKIKYETPIE
                     DAPKKQDQPTTTADEEAERKRREKLRREQERKQEQTKTLLITFGICVAI"
BASE COUNT          562 a          472 c          517 g          638 t
ORIGIN      
        1 gacagaaatc atcagatcaa tgaatttgat aagtgtgttt aaacaggaaa aaggcgtaaa
       61 acgctttttt atgatgatat tcagcatact gcttatgggc ttcggtattt cgcttttctc
      121 ttattccgat atgggcgttg acccgttcac atcaatgaat atggcgataa gcggtaaaat
      181 cggtatcagc ttcggctttt atcagatgtg tatcaactgc gttatcctta ttgcaattgc
      241 ttttctcggc agacatctta ttggcttcgg tacagtagtt aatatggtcg gcgttggtta
      301 tatctgcgaa tttttcactt cgctgtacga cgcacatttg cctgtggaaa aatcccttgc
      361 tttacgctta gtcttaatgc tcttcggcgt aattttcctc tctcttgcgg cttcgatgta
      421 ttttacttcg aatctcggag ttgctcccta tgacgccacg ggctttgtcc ttgatgacag
      481 gactaagatt aaatataaat ggtgcagagt cataacagac cttatctgca cggctgtcgg
      541 atttgctttc ggcggaccgg tcggcatcgg cactgtggtt accgccttta tgatgggccc
      601 ggtggttgcg ttctttaaca agcatgtgtc ggaaaaactg cttgccgcaa aaatgccgag
      661 acttgttgtc cgtgtctatg accttcgccg tttcggcggt ggagtaatat cctcaaacgg
      721 caaattcgca gcataaactc gcttattcca tttcctctct ttttatacac ttttctacgg
      781 cagattgtct gccacggctg ctttttgttc tccacactta aagcagtaaa aaataccacc
      841 gtcaggtggt atttttttgc cttttttcgt gttgtgttat attcggcacg ggtaatgttt
      901 tcatacgggt aaagcctgaa acaagcaact cggctgaaaa aacaaaccga ttgccttatg
      961 ttcgttttct tgacaaatga tgtgtaagtt tgttatcata taaaaaggta tttatgctat
     1021 ttggttttgg aggaaaaaag atgttgaccg ataacttatg tatgggttgt atgagagagc
     1081 ttggggaaga aaaggtttgt ccccattgcg gtttgcatgc ggatttcgtt caggaatctc
     1141 cgcttctgcc gcttagaacg gttatcaacg acagatatat tataggtaaa gccatttcaa
     1201 ccggaggaga cggtgtaaca tatatcgctt gggaccgtga acgcaatgtc gcggtagccg
     1261 taagagagtt tcttccgctt gagcatgtaa tccgtgaatt cggtgatagg gcaataaccg
     1321 ttaaaaagga cagttctctt gtggttcacg acggtataat ggcgtttttg gagctttggc
     1381 gcaaaatcgc aagacttcga gatctctcgg cgttaaagcc cgttgttgat atagttaccg
     1441 aaaataatac tgcgtactcc gtttccgaat acatagagag cataacgctg cgcgattttc
     1501 ttttgaagag ccgcacgggc tatctcggct gggatcaggc aaagacgctt ttgatgcctg
     1561 tgctttcaac tcttgccaca atgcacgagg caggaattta tcaccttggt atttcaccaa
     1621 acacacttct tttgggacgt gacggtaagc tcagactcac gggctttatg atatccgacg
     1681 cgcgacttac aaataccgac tttaacgccg agcttgtaaa gggttatgtt ccctatgagc
     1741 agtatgaccc gaacggcaag gtaggcgcct ggtccgatgt gtacgcgttt acggcagttg
     1801 tttacagaac acttatagga agcactcccg acgattcgag tgagcgcgtg gtgaacgata
     1861 agcttttaat tccggcaaaa ttcgccgagg ttcttcctgc atatcttgtc agtgccatac
     1921 acaatggtct tcagcttcag cccgaggaca gaataagaac cgttgatgat ctccgtgagc
     1981 agctttccgg aagtccaagc accatggcga caaataaaat aaaatatgaa actcccatag
     2041 aggatgctcc caaaaaacag gatcagccta caacaaccgc ggatgaggag gcggagcgta
     2101 agcgccgcga aaagctgcgt cgtgagcagg aacgaaaaca ggaacagacc aaaactctgc
     2161 tcataacctt tggaatttgc gttgcaata
//
LOCUS       DBMDVO010000174         2185 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_23977_length_2185_cov_0.961612, whole genome shotgun sequence.
ACCESSION   DBMDVO010000174 DBMDVO010000000
VERSION     DBMDVO010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23977_length_2185_cov_0.961612"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="ACZPE2_03355"
     CDS             complement(<1..276)
                     /locus_tag="ACZPE2_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1318918.1"
                     /translation="MKITFLGTGTSCGVPTLGCSCKVCRSTNPHDSRLRCAALVDTDT
                     TRLLIDCGPDIRQQLMPLPFRRIDGVLLTHMHYDHVGGIDDLRPYCRF"
     gene            complement(339..1574)
                     /locus_tag="ACZPE2_03360"
     CDS             complement(339..1574)
                     /locus_tag="ACZPE2_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1318919.1"
                     /translation="MKAKKILAGILAASTAVSLAACNSNNATSSTSGSTSSSSKTEDN
                     SSATSGEESSTTPSGEKITLKVLTHRTDRKDDGSLDKRTDAFEEKYNCTVEYQSFKSY
                     ADDVSTMMSTKEYGDVLMIPDTVKLADLSNFFEPLGKYDELKDKYKWADQKMYDGTVY
                     GLAHLGTVSGGICYNKKVWTEAGITEMPKTADELFDDFIFNFAANRKLQYSRIQFPLS
                     VTKFGKKSEMTKKSWRMERFFMTQDYYTLIFDSKRQMSIVKDTTINHVVVEKIYINTN
                     ILKQYVFNRLQGQWKLTGLNVDRLKASNNSSFLKFYKAFVADSAFQVKSINDPLQFSG
                     PDPDDEFNTMTGFLTPEQWPSFVPELPGRLIYNILYGQKYAESSRKIFVMRGIANGLE
                     TELTFECKRGHWKLMQLSM"
     gene            2001..>2185
                     /locus_tag="ACZPE2_03365"
     CDS             2001..>2185
                     /locus_tag="ACZPE2_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318920.1"
                     /translation="MWGKCGIITSDLKSAESGFCRAKARLAAHRTSENILPLNLLSDD
                     KIRRTAVSVYTGLIYGI"
BASE COUNT          605 a          472 c          452 g          656 t
ORIGIN      
        1 aaaacgacaa taaggccgaa ggtcgtctat tccaccgaca tgatcatagt gcatgtgtgt
       61 gagcaacacg ccatcaatgc gtcggaaagg caacggcatt agttgttggc gtatgtcagg
      121 accacaatcg atgagcagac gtgttgtatc ggtatccacc aaagcggcgc aacgcaagcg
      181 gctgtcatga gggttcgtgc tgcgacacac cttgcatgag catcctaacg taggtacgcc
      241 acatgaggtt cccgtaccaa ggaatgttat cttcatctgt atgtttttat tttcttttta
      301 aggctctccc aaaaatccat aaaatcttta agagattatc acatagaaag ctgcatcagc
      361 ttccaatgac ctctcttaca ttcaaatgtc aactcggttt ccaagccatt ggcaatgccg
      421 cgcatcacga aaatcttacg gctactctcc gcatatttct gaccataaag tatattatat
      481 ataagtcggc caggcaattc gggaacaaac gaaggccatt gctcaggggt gaggaaacct
      541 gtcatggtgt taaactcgtc atcgggatca ggacctgaaa actggagcgg gtcattaatg
      601 ctctttacct gaaaagccga atcggcaaca aacgccttat aaaacttaag gaacgaagag
      661 ttgttgctcg ctttcaaacg atcgacattc aaacctgtaa gcttccattg cccctgaaga
      721 cggttgaaga catactgctt taagatgttg gtgttaatat atattttctc aacaacaaca
      781 tgattgatgg tcgtatcctt gactatggac atttgacgct tactgtcaaa aatcaacgtg
      841 taatagtctt gcgtcataaa gaaacgctcc atgcgccacg attttttcgt catttccgac
      901 ttcttgccaa acttcgttac ggacaaagga aactgtatgc gcgaatattg caacttgcga
      961 ttggcggcaa aattgaaaat gaaatcgtca aacagttcat cggctgtctt gggcatttct
     1021 gtaatgcctg cttctgtcca taccttcttg ttgtagcaga taccgccgct tactgtgcct
     1081 aagtgtgcaa ggccgtatac tgttccgtca tacatcttct gatctgccca cttgtactta
     1141 tccttaagct catcgtactt gccgagaggc tcgaagaagt tcgaaagatc ggcgagctta
     1201 actgtatcgg gtatcatgag tacatcgcca tattccttgg tgctcatcat tgtcgaaacg
     1261 tcatctgcgt aactcttgaa tgactgatat tcaacagtgc agttgtactt ttcttcaaaa
     1321 gcatctgtcc tcttgtcgag cgagccgtca tccttacggt ctgtacggtg agtaagaacc
     1381 ttgagtgtga tcttttcgcc cgaaggtgta gtagaagatt cttcccccga tgtcgcagaa
     1441 ctgttatctt ctgtctttga tgaagatgat gtactgcctg atgttgatga tgttgcattg
     1501 ttactgttgc aggctgccag cgaaactgct gtgcttgccg caaggatacc tgcgagtatc
     1561 ttctttgctt tcatgtgata atttcctttc tggtcggaca gtgcttagcc actggtcctt
     1621 agttgatagt taatgaattt agcttatgtt tattaagctg tttccgcttg cttaattcac
     1681 ttaagattat acaacgtttt agctaaaagt caatacggct ataggaaatt cagctaaaac
     1741 acataaataa taattgctta ttatgtgcat taaaaacaaa attcacacta acttaacagt
     1801 gttacacaat tttattaagt aagtatcagt gtaacccgtt ttcacactta caaattcgtt
     1861 taactaaaat tagtttacgt taggttacat atatttcaca ctgtttttag ttgtttttaa
     1921 acgctgttaa attcgcccgt aaatatgata actcaaaaaa tcccggctat tcattaaaaa
     1981 taaaggagat aaagttgcgg gtgtggggga agtgtggtat aataacctca gacttaaaat
     2041 ccgccgagag cggattttgc cgtgcaaaag cacggcttgc ggcgcaccgc acgtctgaga
     2101 acattttacc attaaatctt ttgtctgacg acaaaatacg acgtaccgcc gtgtcggttt
     2161 acaccggctt gatttatggt ataat
//
LOCUS       DBMDVO010000175         2182 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24027_length_2182_cov_1.184046, whole genome shotgun sequence.
ACCESSION   DBMDVO010000175 DBMDVO010000000
VERSION     DBMDVO010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24027_length_2182_cov_1.184046"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(66..1391)
                     /locus_tag="ACZPE2_03370"
     CDS             complement(66..1391)
                     /locus_tag="ACZPE2_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1318921.1"
                     /translation="MMRPNIIFYFSDQQRWDTVNETVTPNLMKLAENGVNFTNSYTCQ
                     PVCGPARACLQTGLYATQNQCYWNGVPLPQNIKPLAEYFNEAGYETAYVGKWHLASDR
                     LPNVGFHCEKSAIPENLRGGYKDWWRASDVLEFTSHGYDGYVFDNENRKVDFKGYRAD
                     CINDFALQYLEQKTSDKPFFMFVSHIEPHHQNDHHCYEGPKDTVDKFKDYPIPPDLSF
                     LPGDYNKMYPDYIAAINRLDDNVGKLVKKLKEKGLYDNTIIIYTSDHGSHFKTRNLEY
                     KRSCHDSATHTPLIISGGMFKGGVVDDRLVSLIDLPPTLLDMAGIPIPKSYSGISLLK
                     EMRENKERDCVFIQISESQCGRAIRTKKYKYSVRTLAPTGYVAHNSDVYFEDYLYDLS
                     VDPIEKNNLIKSPEYASIRAELKDMLINEMVKAGERKPKILPALFVRKK"
     gene            complement(1406..>2182)
                     /locus_tag="ACZPE2_03375"
     CDS             complement(1406..>2182)
                     /locus_tag="ACZPE2_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014035.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1318922.1"
                     /translation="VLILYGFVVFLATLLGSFVGLGGGVIIKPVLDVINAHSLTEISF
                     FSSCAVFAMSITSTTRHIIKKTPIKPSIVLLVALGSVGGGLLGNYLFNYALNVSSTPN
                     TVRGIQSAILAVFLVCVIIFVSGNFKTFNVKNPIAILLSGLLLGTAAAFLGIGGGPIN
                     VAFLTLMFSFTMRDAAVYSVAVIFFSQCANIISTYIKTGFSGFDMKVLLVIIPVAVIG
                     GFIGSILNRKCNEKAIKVTFTIAVSSVAALSLYNAVMSFI"
BASE COUNT          662 a          467 c          410 g          643 t
ORIGIN      
        1 agaaagcgga aaacaaattt tttcttttaa agttaaatat ttcttttcca ttcccttaac
       61 tcctgttatt tttttcttac aaataaagcc ggtaaaattt ttggcttacg ctcccctgct
      121 ttgaccattt cgtttatcag catatctttg agttcagccc ttatggaagc atactccgga
      181 gattttatta ggttgttttt ctcaatcgga tcaactgata gatcatataa ataatcttca
      241 aaatacacat ctgagttgtg ggcaacatat ccggtaggag caagagtcct tacggaatac
      301 ttgtactttt tggtgcgtat agctcttccg cactgacttt cgcttatttg aataaatacg
      361 cagtcgcgtt ccttattttc acgcatttcc ttgagcagcg atatgcccga atagcttttc
      421 ggtatcggta ttcctgccat atcaagcaat gtcggaggaa gatctataag actaacaagc
      481 ctgtcatcaa ctacaccgcc tttgaacatc cctccgctga taatcagcgg cgtatgcgtt
      541 gcgctgtcgt ggcaacttct cttatactca agattgcggg tcttaaaatg agagccgtga
      601 tcgcttgtat aaataataat tgtgttatcg tagagtccct tttccttcag ctttttaacg
      661 agcttaccga cattatcatc cagtctgttt attgcagcta tgtaatccgg atacatttta
      721 ttataatcgc cgggtaaaaa cgacaagtcg ggtggtatag gatagtcttt aaatttatct
      781 acagtatcct tagggccttc ataacaatga tgatcatttt gatgatgtgg ttcaatatga
      841 gatacaaaca taaaaaacgg cttatccgat gtcttttgct caagatattg aagggcgaaa
      901 tcgtttatgc aatcagcgcg gtagcccttg aaatcaactt ttctgttttc attgtcaaaa
      961 acatatccat cgtatccgtg tgatgtaaac tcaagtacat cggatgcacg ccaccaatct
     1021 ttataaccgc cacgcaggtt ctccggaatt gcgctttttt cgcagtggaa gcctacattc
     1081 ggcagtctgt cggaagctaa gtgccacttc ccgacataag ctgtttcgta tcctgcttcg
     1141 ttgaagtatt ccgctaacgg cttaatgttt tgcggaagcg gaactccatt ccaatagcat
     1201 tggttttgcg tcgcataaag accggtttga aggcaagcgc gagccggtcc gcaaaccggt
     1261 tgacacgtat atgaatttgt aaaattcaca ccgttttcgg caagcttcat aagatttgga
     1321 gtaacggttt cattaacagt gtcccatctc tgttgatcgc tgaaataaaa aataatattt
     1381 ggcctcatta tgttcctccg ttatattata taaatgacat tacagcattg tataaagata
     1441 gtgccgcaac cgaagatacg gcaattgtaa acgtgacttt aattgctttt tcattgcatt
     1501 ttctatttaa aatcgaacct atgaaccctc cgataaccgc aacagggata atcacaagca
     1561 gtaccttcat atcgaatccg gaaaagcccg tttttatata tgtggatata atgtttgcgc
     1621 attgggaaaa gaaaataaca gccaccgaat acaccgctgc atctctcatc gtaaacgaaa
     1681 acataagagt taaaaaagca acatttatcg gtccgccgcc aatacccaga aatgccgccg
     1741 cggtaccgag cagtaatccg gacagcaaaa ttgctatggg atttttaaca ttaaatgttt
     1801 tgaaattacc ggatacaaaa ataataacgc acactaaaaa aacagccaat attgcagact
     1861 gtatgcctct tacggtattc ggagttgaag aaacatttaa tgcataatta aaaagataat
     1921 ttccgagcag accgccgccg accgagccca aagcaaccaa caacacaata gacggcttta
     1981 tcggcgtttt tttaatgata tgacgtgtag ttgatgttat cgacatcgca aaaacagcac
     2041 aagacgaaaa gaaagaaatt tcggttagag agtgtgcatt gataacatcg aggaccggct
     2101 tgattataac tccgccgccg agtcctacaa acgaccctaa aagtgtcgcc aaaaaaacaa
     2161 caaaaccata taaaattaaa ac
//
LOCUS       DBMDVO010000176         2173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24154_length_2173_cov_1.377896, whole genome shotgun sequence.
ACCESSION   DBMDVO010000176 DBMDVO010000000
VERSION     DBMDVO010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24154_length_2173_cov_1.377896"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="ACZPE2_03380"
     CDS             <1..265
                     /locus_tag="ACZPE2_03380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1318923.1"
                     /translation="GNTSGEIAARGVNKASGIKEYLDCCGGDINDAFAFGDAENDVEM
                     FKSVNTGIAMGNACDELKRLASYITADITDDGLYKAFAHFGLI"
     gene            complement(332..2041)
                     /locus_tag="ACZPE2_03385"
     CDS             complement(332..2041)
                     /locus_tag="ACZPE2_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1318924.1"
                     /translation="MFFEKLYNLIYHIGFTAGSGFMKFGKWLSRGLSRPVKTVGAVFF
                     AVFLVIDKFILKTFKTVSAEFKLLMADIKRVSSGLNSLIHTDRKSAEAKLRIYIKKAF
                     SRHGIVFTFAVNLLVPIICLGVLCSTIGYWNKATLALEIKYNGSVIGYAKNESEYLDA
                     EQQAKTRLKTAASASGKEQELIKPAQYQLKVVNKTDLTDSSSMCDKLIENSDSNITNA
                     CGIYINGDFLCAVKNETDATSVFDKILSDYKIDDPNAAKSFVENITYEQGLYPDNEAT
                     VWDAEKLSAKLKSKKSEAVYYTVKDGDTVSGIASANGLTVSELAQLNPGLSETIRSGD
                     KLLISSEVNYVRVQVTKTETHNEAIAYTSTNVNNANLYTGTKKVTTKGVNGVQQVTEL
                     VTYIDGTRVSSKEVSRVTLSEPVNEVIQIGTKAPITGRGSSGGKVITTGGRFVWPAIG
                     ATSVSSGYGGARRHGGIDIVKPGGGSTGLTIVAAASGTVTTATYHPSWGYYIVINHGN
                     GLSTLYAHTLPGSFKVRVGQKVSAGQPIANIGSSGNVTGPHLHFEVYVNGRRTNPMPY
                     LGR"
BASE COUNT          541 a          517 c          495 g          620 t
ORIGIN      
        1 cggcaataca agcggggaga ttgcggcgcg cggagtcaac aaagccagcg gaattaaaga
       61 atatctcgat tgctgcggcg gagacataaa cgacgcattc gcattcggcg acgcggaaaa
      121 cgatgtcgaa atgtttaaga gcgtaaacac gggcatcgca atggggaatg cctgtgacga
      181 gctcaaacgc ttggcctcgt atataaccgc cgatatcacg gatgacggtc tatacaaagc
      241 gtttgcgcat ttcggtttga tttaaatttt gattataaag caaaaaagca cgtttgagtt
      301 attgctcaaa cgtgctttta ctgtttattt gttatcttcc gagatacggc atgggattag
      361 ttcgtctgcc gtttacatag acctcaaagt gaagatgcgg tccggttacg ttacccgagg
      421 agcctatatt cgcaataggc tgacctgccg aaaccttttg accgacacgc accttaaacg
      481 agccgggtaa ggtatgtgca tagagcgttg aaaggccgtt gccgtgattt atgacaatat
      541 aatagcccca actcggatga tatgttgccg tggtaacggt tcccgaagcc gccgctacta
      601 ttgtaagacc ggtgcttccg ccgcccggct ttactatatc tataccgccg tgacgtcttg
      661 ctccgccgta gcccgaagaa acggacgttg cgcctattgc cggccaaacg aatctgccgc
      721 ctgttgtgat gaccttgccg cctgagctgc ctcttccggt gatcggtgcc tttgtgccta
      781 tctgtataac ctcgtttaca ggctccgaaa gagtcactct tgaaacctct ttcgatgaaa
      841 cacgagtacc gtcaatatag gttacaagct ccgtaacctg ctgaactccg ttcacaccct
      901 ttgttgtaac cttttttgtg cctgtataga gattggcatt atttacgttt gtgcttgtat
      961 aggctattgc ttcattatga gtttcggtct ttgtgacctg cactctgaca tagtttacct
     1021 cactcgaaat aagcagcttg tcgccggaac gaatcgtttc ggaaaggccg ggattcagct
     1081 gcgccaattc ggaaacggtg agcccgtttg cggaagcgat tcccgaaacg gtatctccgt
     1141 cctttaccgt gtaatacacc gcttcgctct tttttgattt gagcttggcg gatagctttt
     1201 cagcgtccca aaccgtggcc tcattatcgg gataaagccc ctgctcatat gttatgtttt
     1261 caacgaagct ctttgccgcg ttggggtcgt ctattttata atcgctgagt atcttatcga
     1321 aaacgcttgt agcgtccgtc tcatttttaa cggcgcacaa gaaatcgccg ttaatatata
     1381 tgccgcaggc attggtgata ttgctgtctg aattttctat gagcttatcg cacattgacg
     1441 aggagtcggt aaggtcggtc ttatttacga ccttgagctg atactgcgca ggcttaataa
     1501 gctcctgctc cttaccggag gcagacgcag cggttttgag ccttgttttg gcctgctgct
     1561 cggcatcgag atattccgac tcgtttttgg catagcctat tacggagccg ttatatttta
     1621 tctcaagagc gagcgtggct ttgttccaat agcctatcgt ggagcaaagc acaccgaggc
     1681 aaataatcgg aacaagcaga tttaccgcaa aagtaaacac tatgccatgc ctcgaaaatg
     1741 ccttctttat ataaatacgc agcttcgctt cggcggattt tctatctgta tggataaggg
     1801 aatttaagcc tgagctgact ctttttatat ctgccataag gagcttgaat tccgcgctta
     1861 cggttttgaa ggtcttgagt ataaatttgt caatcacaag aaacacggca aaaaataccg
     1921 ctccgactgt ttttacggga cgcgaaagcc ccctcgacaa ccatttcccg aatttcataa
     1981 atccggagcc tgccgtaaaa cctatgtgat atattaagtt gtaaagcttt tcaaaaaaca
     2041 taacagcctc acaataaaat agtaaaagtt attttaccat acaatgcgac aaatttcaac
     2101 cttacgcttt tacatacccg ttgccgaaca tcttatctat ttggcgtttt attcctttgt
     2161 gctccggcaa aag
//
LOCUS       DBMDVO010000177         2170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24210_length_2170_cov_1.218463, whole genome shotgun sequence.
ACCESSION   DBMDVO010000177 DBMDVO010000000
VERSION     DBMDVO010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24210_length_2170_cov_1.218463"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="ACZPE2_03390"
     CDS             <1..589
                     /locus_tag="ACZPE2_03390"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1318925.1"
                     /translation="REITDRYNGDKYLSEAVRACPGIRILRQEPWEALCSFIISQNNN
                     IPRIKGIIKRLCESFGEELESGMYTFPSPEVLSGKAPEDLASLRAGFRAKYIIDAANK
                     VSSGEIDFDKIAASPIEFGRQELQKIKGVGKKVAECTLLYGFGKYEAFPEDVWVKRIM
                     AEMYPDGLPACTKGSEGIAQQYLFHWRRTVYRENL"
     gene            607..1080
                     /locus_tag="ACZPE2_03395"
     CDS             607..1080
                     /locus_tag="ACZPE2_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1318926.1"
                     /translation="MITDKPDIFDRIMSLKIFKWANPFYTKYKEVLMYLLFGGLTTLV
                     SVGIFALFESHFGLNEHISNVISWLTAVLFAFVTNRIWVFPTKTKGFAAFIFQMAKFY
                     GGRLFTLGVEELMLFVFVTKLQINAVLIKLIAQVVVVILNFIISKLFVFKKNKNS"
     gene            complement(1151..>2170)
                     /locus_tag="ACZPE2_03400"
     CDS             complement(1151..>2170)
                     /locus_tag="ACZPE2_03400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1318927.1"
                     /translation="EMDMLLTSGEQISIALLSMAIEKLGCPSVSLLGWQAGFRTSSAY
                     GSARIKSIKTDRLRAEVDRHNIVVVAGFQGINKYDDLTTLGRGGSDTSAVAIAAAMHA
                     DRCQIFTDVEGVFTADPRKIPEARKLKEITYDEMLELATLGAQVLNNRSVEMAKKYNV
                     EIEVLSSLKRVPGTIVKEVAKMEKMLVRGVTKDTDVARISVTNIDNTPGIAFKLFSKL
                     AQKGINVDIILQSVGRDGTKDITFTVAKDNAVDAIAAVHETFDIDDKNITCATNVAKI
                     SVVGAGMESHPGTASKMFEALYEHDINIDMISTSEIKISVLIDAQDADRAVAAVHKAF
                     YPELN"
BASE COUNT          545 a          468 c          541 g          616 t
ORIGIN      
        1 ccgtgaaatt accgatcgct acaatggcga caaatatctg tcggaggccg taagagcttg
       61 ccccggtatc agaattttgc gtcaggagcc gtgggaggcg ctctgctcgt ttataatttc
      121 gcaaaacaac aatattccgc gtataaaggg aataataaaa aggctctgtg agagctttgg
      181 cgaagagctt gagagcggaa tgtacacatt tccctccccc gaagtgctgt ccggcaaagc
      241 acccgaggac ttggcttcgc ttcgcgccgg tttcagggca aaatacataa ttgacgccgc
      301 aaataaggtt tcgtcgggtg aaatcgactt tgacaagatt gccgcttcgc ccatagaatt
      361 cggcaggcag gagctgcaaa aaataaaagg cgtgggcaaa aaggttgccg aatgtacgct
      421 tctgtacgga ttcggaaaat acgaggcgtt ccccgaggac gtgtgggtaa agcgcataat
      481 ggcggagatg tacccggacg gtcttcctgc ctgtacaaag ggcagcgagg gcatagctca
      541 acagtatttg tttcattgga ggcgcacggt gtaccgcgaa aacctataac ggcggaggag
      601 ttttttatga taacagacaa gcccgatatt tttgacagaa taatgtcgct taaaatattt
      661 aaatgggcaa acccgtttta cacaaaatac aaggaagtgc tgatgtatct gctgttcggc
      721 ggacttacaa cgcttgtaag cgtaggcata tttgcactgt ttgagagtca cttcgggcta
      781 aacgagcata tttcaaacgt catatcttgg ctgactgcgg tactgtttgc gtttgtgaca
      841 aaccgcatat gggttttccc gacaaagact aaaggttttg cggcttttat ttttcagatg
      901 gcaaagtttt acggcggcag gctgtttacc ttgggcgttg aggagcttat gctctttgta
      961 tttgtaacaa agcttcaaat caatgccgtt ttgattaagc tcatagcgca ggtcgtagtc
     1021 gtgatactga attttattat aagtaagctg tttgttttca aaaaaaataa aaattcttaa
     1081 taaggctttt tgcttaaaaa aataacctcc gcatttcata tgcagaggtt gtttttttag
     1141 ttatagggat ttagtttaat tcgggataaa atgccttgtg aactgccgca acggctctgt
     1201 cggcgtcctg tgcgtctata agaacggaga ttttgatttc gcttgttgaa atcatatcaa
     1261 tatttatgtc gtgctcatag agtgcctcga acatctttga agcggttccg gggtgagact
     1321 ccataccggc accgacaacg gagatctttg caacgtttgt ggcacaagtg atgtttttat
     1381 catcgatatc aaaggtctcg tgaacggctg ctattgcgtc aaccgcatta tccttggcaa
     1441 cggtaaaggt gatgtccttt gtgccgtcgc gacctacgga ctgaagaatt atatcgacgt
     1501 ttatgccttt ctgtgcaagc tttgagaaaa gcttgaatgc aataccgggg gtattgtcta
     1561 tatttgtaac cgaaattctt gcgacgtcgg tatcctttgt gacgcctctt acgagcattt
     1621 tttccatttt agcgacctcc ttgactattg tgccgggtac acgtttaagg cttgagagta
     1681 cctctatttc aacattgtat ttttttgcca tttccacgga acggttattg agaacctgcg
     1741 cgccgagcgt tgcaagctca agcatttcgt cgtaagtaat ttccttgagc tttctcgcct
     1801 cgggtatctt tctcgggtcg gcggtgaata cgccctcaac atcggtgaat atctgacaac
     1861 gatccgcgtg catggcggct gctatggcaa cggcactcgt atccgagccg cctctgccga
     1921 gcgttgtgag gtcatcgtat ttgtttatac cctggaagcc tgcgacaaca actatgttgt
     1981 ggcggtcaac ctcggcacgt aaacgatcgg ttttaattga tttgattctt gccgagccgt
     2041 aagccgagct tgttctgaag cctgcctgcc agccgagaag tgaaaccgag gggcagccga
     2101 gcttttcaat agccattgaa agaagcgcga ttgaaatctg ctcgccggaa gttaggagca
     2161 tatccatctc
//
LOCUS       DBMDVO010000178         2169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24227_length_2169_cov_1.261122, whole genome shotgun sequence.
ACCESSION   DBMDVO010000178 DBMDVO010000000
VERSION     DBMDVO010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24227_length_2169_cov_1.261122"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /gene="glyA"
                     /locus_tag="ACZPE2_03405"
     CDS             <1..145
                     /gene="glyA"
                     /locus_tag="ACZPE2_03405"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571183.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1318928.1"
                     /translation="VTTRGLDEADMEKIAEYIYLCATDFEGKADYIREGVNDICARHP
                     LYK"
     gene            192..722
                     /locus_tag="ACZPE2_03410"
     CDS             192..722
                     /locus_tag="ACZPE2_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1318929.1"
                     /translation="MRDVIIASANRKSAEHIKSILQRDLLFVSEIYQSGAEVLSYASI
                     RPDAVVVCGRLSDGLPAVSLAETLPPGFDVVHLVSSSDAYQGFVSNMVELTMPIDRVE
                     FVSVVRTLTQLSSDITSRKKTRSVEENDLLTLAKQRLCENYGISEREAHRKIQKMSME
                     QGVRLMDVARKILEED"
     gene            742..1587
                     /gene="dapF"
                     /locus_tag="ACZPE2_03415"
     CDS             742..1587
                     /gene="dapF"
                     /locus_tag="ACZPE2_03415"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1318930.1"
                     /translation="MKFTKMQGAGNDYIYVNCFEEKVSDPESTAVKVSDRHFGIGSDG
                     LVLIEPSDKADFKMDIYNADGSRAKMCGNATRCVAKYVCDRGLTNKDEISLETLSGIK
                     YIKVFKGDDGKVKSARVDMGAPVLSGKKIPTLFDGDKVVGETLHLGGELGDKIVTCVS
                     MGNPHCITYVDDVKTLDLAKIGPVFENNEMFPDRVNTEFIHIISETEFDMRVWERGSG
                     ETLACGTGACASAVASVLNGYCLRNTDIKVNLIGGTLYINWTNDGNVLMTGPAAEVCT
                     GEIDV"
     gene            1611..>2169
                     /locus_tag="ACZPE2_03420"
     CDS             1611..>2169
                     /locus_tag="ACZPE2_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1318931.1"
                     /translation="MNINENFLKIESSYLFSNIAKKVKAYTKANPDKEIIRLGIGDVT
                     RPLAPACIEAMHKAVDEMADEKTFRGYPPEYGYDFLLNAINENDYRSRGIELDNSEIF
                     VSDGAKSDTGNIGDIFSLDNKVAICDPVYPVYVDTNVMSGRSGEKDENGEEILVSIED
                     DEEFDRIADIFDDEFADIDYDMEPED"
BASE COUNT          620 a          484 c          578 g          487 t
ORIGIN      
        1 agtcaccaca cgcggtcttg acgaagcgga tatggaaaaa attgccgagt atatatatct
       61 ttgcgccacc gattttgagg gtaaagcaga ctacatcaga gagggcgtaa acgatatttg
      121 tgcgcgccat cccctttaca aataattttt aactaaacga agaacggggg aaggcttaac
      181 gccgtctgag gatgagagac gtcataatcg caagcgcaaa ccgcaaatcc gcggagcata
      241 taaagagcat tctccagcgg gatttgctct ttgtttcgga aatatatcag tccggcgcgg
      301 aggtgctctc ttacgcgagc atcagaccgg acgcggtagt tgtgtgcgga cgtctgtccg
      361 acggcttacc ggcagtctct cttgcggaaa cgcttccccc cggcttcgac gttgtgcatt
      421 tggtgtcgtc ctcggacgca tatcagggct ttgtgagcaa tatggtggag cttaccatgc
      481 cgattgacag ggttgagttt gtatctgtcg tgcggacgct tactcagctt tcgagtgata
      541 ttacatcacg caaaaaaaca cgctccgtcg aggaaaacga ccttttgacg ctggcaaaac
      601 aacgcctttg cgaaaattac ggtatctcgg agcgcgaggc acacaggaaa attcagaaaa
      661 tgagtatgga acagggcgtc cgccttatgg acgtcgcaag aaaaattctc gaagaagatt
      721 agttaggacg aggtggtttc tttgaaattc acaaagatgc agggcgcagg aaacgattat
      781 atatacgtaa actgctttga ggaaaaggtt tccgaccccg agagcacggc tgtaaaggtc
      841 agcgacaggc atttcggaat aggctcggac gggcttgttt tgatagagcc gagcgacaaa
      901 gccgatttta aaatggatat atacaatgcc gacggttcaa gggcgaaaat gtgcggcaac
      961 gcgacaagat gcgttgcaaa atacgtgtgt gaccgcggac ttacaaataa ggatgaaatt
     1021 tcgcttgaaa cgctcagcgg aattaaatat ataaaagttt tcaagggcga cgacggcaag
     1081 gtgaaaagtg ccagagtcga tatgggagca cccgttcttt ccggaaaaaa aatccccacg
     1141 ctgtttgacg gcgacaaggt tgtgggtgag acgctgcacc tcggcggcga attgggcgat
     1201 aaaatcgtta cgtgcgtttc aatgggcaat ccgcattgca ttacctatgt ggacgacgta
     1261 aaaacgcttg acctcgcaaa aataggtcct gtatttgaaa acaacgagat gttccccgac
     1321 agggtgaaca ccgaatttat acatataata agcgaaaccg aatttgatat gcgtgtatgg
     1381 gagcgcggct cgggcgaaac actcgcctgc ggaaccggag catgcgcctc cgccgtggca
     1441 agcgtgctga acggctactg cctcagaaat accgacatca aggtaaatct cataggcggt
     1501 acactctata taaattggac aaatgacggc aatgtgctga tgaccggccc ggctgccgaa
     1561 gtctgcacgg gcgaaataga cgtgtaattt aatttgttac ggaggacaaa atgaatatta
     1621 acgaaaactt ccttaaaatc gaaagcagct atcttttttc aaatatcgca aaaaaggtaa
     1681 aggcttacac aaaggcaaat cccgataagg agattataag actcggcatc ggcgacgtaa
     1741 caagaccgct tgcacctgcc tgcatcgagg ctatgcacaa ggctgttgac gaaatggccg
     1801 acgaaaagac gttccgcggc tatccgccgg agtacggcta tgatttcctt ttgaacgcga
     1861 taaacgaaaa cgactaccgt tctcgcggca ttgagcttga caattccgaa atattcgtgt
     1921 cggacggcgc gaagagcgac acgggcaaca taggcgacat cttttcgctt gacaacaagg
     1981 ttgcgatttg tgaccccgtt taccccgtat atgtcgatac aaacgtaatg tcgggccgtt
     2041 cgggcgaaaa ggacgaaaac ggcgaggaaa ttcttgtttc cattgaggac gatgaggagt
     2101 tcgaccgcat tgccgatatt ttcgacgatg agtttgcgga tatcgactat gacatggagc
     2161 ccgaggact
//
LOCUS       DBMDVO010000179         2167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24266_length_2167_cov_0.995160, whole genome shotgun sequence.
ACCESSION   DBMDVO010000179 DBMDVO010000000
VERSION     DBMDVO010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24266_length_2167_cov_0.995160"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="ACZPE2_03425"
     CDS             complement(<1..1222)
                     /locus_tag="ACZPE2_03425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1318932.1"
                     /translation="MKIAVVCDSFKGSLTSKDACAAVKDGLLRCNKNFEVLSLPFADG
                     GEGTSRCFYDILGGQLRKAAVHDPLLREITAEYTVLPDGTAVIDVASASGLTLLKSSE
                     RDAVKVSSLGSGELICDAAEHGAKHIILGLGGSATTDAGTGILYALGMRFFSEDGVEV
                     LPDGQNMIRVKKIRRTENFERFKDIKFTLACDVTNPLCGENGAAYVFSPQKGASKNDV
                     ELLDDGLRNIGEIFEKASGKKIINLPGAGAAGGIGGGLSAFLNCELQSGFDVLARAAS
                     LEDKIRRSDAVITGEGKTDRQTLFGKLPKRVSDIAEKFGVPCILLSGDVENDISAEKL
                     GVCEIHKIKENGISLEHCMKNAASLLSERAFAIGKDSKIINKQYSRKQMLCHQFMQLI
                     REHSTCEHQVPFYTE"
     gene            complement(1225..>2167)
                     /locus_tag="ACZPE2_03430"
     CDS             complement(1225..>2167)
                     /locus_tag="ACZPE2_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HIR1318933.1"
                     /translation="TAAKIKEMMPSARIAVAHGRMGEEELSEIWRDLLEGDIDILVCT
                     TIIETGVDVPNCNTLIIEDADRLGLAQLHQIRGRVGRSTRRASAYFTFKRGKELSEIA
                     NRRLSAIREYTEFGSGFKIAMRDLEIRGAGNVLGAQQHGHMEAVGYDMYLQLLGEAVE
                     EEKGEKPISRKKECLIDMQIDAHIPDNYIKSIPQRLAVYRRIADIKNTEDAEDVKDEL
                     RDRFGEIPQSVQGLIDVSLLRNTAAAKGIYEIGQKGNSVILYVNDIDTNTVLNLSSMM
                     RGRVSIMDYGKKHIAVKIADGQSPLDTLKEIFGYMVF"
BASE COUNT          500 a          613 c          470 g          584 t
ORIGIN      
        1 gttccgtata aaacggaact tgatgttcgc aagtactgtg ttcgcgtatc agttgcatga
       61 actggtggca gagcatctgt tttctgctat attgcttatt tattattttt gagtctttgc
      121 ctatcgcaaa ggctctttcg gacaaaagcg aggcggcgtt tttcatacaa tgctcaaggc
      181 ttataccgtt ttcctttatt ttatgtattt cgcatactcc gagtttctcg gcggaaatat
      241 cgttttcgac gtcgccgctc aaaaggatac acggaacacc gaatttttcg gctatgtccg
      301 agaccctctt aggaagcttg ccgaataggg tttgcctgtc cgtcttgccc tcgcctgtta
      361 tgaccgcatc ggagcgacgt attttatcct caagcgatgc agcccttgcc agcacatcaa
      421 atccgctttg aagctcacag ttcaaaaagg cggataatcc accgccgatg ccgcccgcag
      481 ctccggctcc cggaagattt ataatctttt tgccggacgc tttttcaaaa atctctccta
      541 tatttcgtaa gccgtcatcc aaaagctcca catcgttctt cgaggctccc ttttgcggag
      601 aaaaaacata agccgctccg ttttctccgc acagaggatt tgtgacgtca cacgcaagag
      661 tgaactttat atccttgaat ctttcaaaat tctcggttcg ccgtattttc ttaactctta
      721 tcatattttg accgtcgggc aaaacctcga cgccgtcctc cgaaaagaac cgcataccga
      781 gggcatacaa aatgcccgtc cccgcatccg tggtagcact gccgccgagt ccgagaatta
      841 tatgttttgc cccatgctcc gcagcgtcgc aaatcagttc tccgcttccc aatgacgaca
      901 ccttcacggc gtcgcgctcg ctgctcttga gaagcgtcag cccgcttgcc gaggctacat
      961 cgattacagc ggtaccgtcg ggcaaaacgg tgtattccgc cgtgatttca cgaagcagag
     1021 ggtcgtgcac cgccgctttt cgaagctgcc ctccgagtat atcgtaaaaa cagcgagagg
     1081 tcccctctcc gccgtcggca aacggcagag aaagcacctc aaaatttttg ttacaccgaa
     1141 gaagcccgtc tttaaccgcg gcgcaagcat cctttgacgt aagactccct ttaaaagagt
     1201 cgcaaacaac agctatcttc ataatcagaa caccatatat ccgaatattt ccttgagcgt
     1261 atcgagcggc gactgaccat cggcaatctt taccgcgatg tgctttttgc cgtaatccat
     1321 tatagatact ctgccgcgca tcatggagga caggttcaaa accgtatttg tatctatatc
     1381 gttcacataa aggataacgg aattgccctt ttgccctatt tcgtatatac cctttgcggc
     1441 ggcggtatta cgaagaagcg aaacatcgat aagtccctga accgactgcg gaatttcacc
     1501 gaatcggtcg cgaagctcat ccttaacgtc ctctgcatcc tcggtatttt taatgtccgc
     1561 aattctgcga taaaccgcaa gcctctgagg aatgctcttg atgtaattat cgggaatgtg
     1621 agcgtctatc tgcatatcta tgagacactc ctttttacgc gaaatcggct tttcgccctt
     1681 ttcctcctcc acggcttcac cgagaagctg caagtacata tcgtagccta ccgcctccat
     1741 atggccatgc tgctgcgcac cgaggacgtt gcccgctccg cggatttcaa ggtcacgcat
     1801 ggcaatctta aagccggagc cgaattcggt atattccctt attgccgaaa gtcgtctatt
     1861 cgctatttcc gacaattcct tgccacgctt aaacgtgaaa tatgccgacg ctctgcgtgt
     1921 gcttctgccg actcttccgc gtatctgatg aagctgtgcg agccctagcc tgtcggcgtc
     1981 ctcgataatc agcgtattgc aattcggaac atcaacgccc gtctcaatga tggtggtgca
     2041 aacgagtatg tctatgtcgc cctcaagcaa gtcacgccag atttcagaca gctcctcctc
     2101 gcccattcgg ccgtgcgcca cggctatacg agccgacggc atcatttcct ttatcttagc
     2161 cgccgtg
//
LOCUS       DBMDVO010000180         2166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24285_length_2166_cov_1.085230, whole genome shotgun sequence.
ACCESSION   DBMDVO010000180 DBMDVO010000000
VERSION     DBMDVO010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24285_length_2166_cov_1.085230"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="ACZPE2_03435"
     CDS             complement(<1..550)
                     /locus_tag="ACZPE2_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HIR1318934.1"
                     /translation="MKKLFKNKIFKGVVGTLLCVCILLFAAVIGINAYMISFSKKNFV
                     TVDELKADSYDCILVLGAGLWSGKPSPMLKERLDFSLEAYNKECSDRFLVSGDHGTKN
                     YDEVNAMKDYLVENGISADCVYIDHAGFSTYESMYRARDVFKVQKVLIVTQKYHLYRA
                     VYIARQLGIEAYGLDREELKYPV"
     gene            complement(590..1606)
                     /gene="iolC"
                     /locus_tag="ACZPE2_03440"
     CDS             complement(590..1606)
                     /gene="iolC"
                     /locus_tag="ACZPE2_03440"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIR1318935.1"
                     /translation="MKYIEFDSSKKYDLILLGRIAVDLNPVDYYCPLNESTTFKRYLG
                     GSPANIAVGLARLGKKCGFFARVSDDQLGTFVTDFFEEEGIDTSRVKRCENGEKIGLT
                     FTEIKSETESSIVMYRNDAADLKLEVSDIDEEYIKQGKALLISGTALCESPSREAALK
                     AVMLAKRNCIPIIFDIDYRAYNWKNDDEISIYYSAVAREADIILGSREEYDLTEKLIK
                     QGMSDEETAKYWHSQNAKIVVIKHGKEGSTAYTNDGESYNIKPFPVKLLKSFGGGDGY
                     ASAFLYGLFEGYDIIDALEFGSASAAMLVASHACSQDMPSVEAVKDFIKKEKEEYGEM
                     IARV"
     gene            complement(1621..>2166)
                     /locus_tag="ACZPE2_03445"
     CDS             complement(1621..>2166)
                     /locus_tag="ACZPE2_03445"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1318936.1"
                     /translation="FTSVMLDGSLLPLEENIKTVKGVVETAKEYGATVEAELGVVGGN
                     EGEGKSHKILCTNPDDAKRFCDETGVDALAVAIGNAHGNYPVLPELRFDVLEEIHKKV
                     NTPLVLHGGTGISDEQFQRAISLGVRKINIATASFDSLASYAKTYCDNKEKANYFELS
                     ACEVEGVYQNVKRHIKVFNME"
BASE COUNT          587 a          519 c          430 g          630 t
ORIGIN      
        1 tgacgggata tttgagctcc tccctgtcaa gaccgtaagc ctcaatgcca agctgtcttg
       61 caatatacac agccctgtaa agatggtatt tttgcgttac gataagcacc ttttggacct
      121 tgaaaacatc tcttgcccga tacattgact cataggttga aaagcctgca tggtcaatat
      181 acacgcaatc agccgaaata ccgttttcaa caaggtaatc cttcatagcg tttacctcgt
      241 catagttttt cgtgccgtga tcccccgaga caaggaatct gtcgctacac tctttattgt
      301 atgcttcaag agaaaaatca agtctttcct ttagcatagg cgacggcttg ccgctccaaa
      361 gccctgcacc aagtaccaaa atacaatcat agctgtccgc ttttaattcg tctacggtaa
      421 caaaattctt tttagaaaaa gaaatcatat atgcgttaat gccgattact gccgcaaaaa
      481 gcaaaataca aacacataaa agcgttccga caacaccttt gaaaatcttg tttttgaaca
      541 gttttttcat tttgagcctt cctgcaaatc aaaaagggag cggaatgatt tacacccttg
      601 caatcatttc accgtattct tctttttctt tcttaataaa gtccttgacc gcttcgacag
      661 agggcatatc ctgtgagcaa gcgtgagaag ctacaagcat agccgccgaa gccgagccga
      721 attcgagagc gtcgattata tcatagccct caaaaagtcc gtaaaggaat gcggaagcgt
      781 aaccgtctcc gccgccgaag gatttaagga gtttaacggg gaacggctta atgttatagc
      841 tttcgccgtc atttgtataa gctgtcgagc cctccttacc gtgcttgatt accacgattt
      901 tagcattttg cgaatgccaa tatttagctg tttcttcatc ggacataccc tgctttatga
      961 gcttttcggt aaggtcgtat tcctcacgag agccgagaat aatatcagcc tcacgggcaa
     1021 ctgcggagta atagattgaa atttcatcgt catttttcca attatatgca cggtagtcaa
     1081 tatcaaaaat aatcggaata caatttctct ttgcaagcat aacagccttg agcgccgcct
     1141 ctctcgacgg gctttcgcaa agagcggtgc ctgaaatcaa aagagccttg ccctgcttga
     1201 tgtattcctc gtcaatatcg gaaacctcca atttcaagtc ggccgcatcg ttgcgataca
     1261 taactatgga actttcggtt tcggatttga tttcggtaaa tgtaagaccg attttctcac
     1321 cgttttcaca tctcttaact cttgaggtgt caattccctc ttcctcaaag aaatcggtta
     1381 cgaatgtgcc gagctggtcg tcggaaacac gggcaaaaaa tccgcatttt ttaccgagcc
     1441 tcgcaagtcc taccgctata tttgcgggag agccgccgag gtatctctta aaggtggtgc
     1501 tttcgttaag agggcagtaa taatcgacgg gattcaagtc aaccgcaatt ctgcccaaaa
     1561 gaataaggtc atattttttt gaagaatcaa attctatgta tttcataaat gtctccttaa
     1621 ttattccata ttgaaaactt taatatgtct tttaacattt tgataaacgc cctcaacctc
     1681 acaggcggag agctcaaaat aattggcttt ttccttgtta tcacaataag tctttgcata
     1741 agatgccaag ctgtcaaagg aagctgttgc tatatttatc ttgcgaacac cgagtgaaat
     1801 cgccctctga aactgctcgt cgcttatacc cgtaccgccg tgaagcacaa gcggtgtatt
     1861 gactttttta tgtatttcct caagcacgtc aaagcgaagc tcgggaagta cgggataatt
     1921 tccgtgcgca ttgcctattg ccacggcaag tgcgtcaacc cccgtttcat cacaaaagcg
     1981 tttagcgtca tcggggtttg tgcaaagaat tttgtggctt ttgccctcgc cctcgtttcc
     2041 gccgacaact cccaattcgg cttcgacggt tgcgccgtat tcctttgcgg tttcaacgac
     2101 gccttttacc gttttaatat tttcctcaag cggtaaaagc gagccgtcaa gcattaccga
     2161 cgtaaa
//
LOCUS       DBMDVO010000181         2164 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24309_length_2164_cov_1.220553, whole genome shotgun sequence.
ACCESSION   DBMDVO010000181 DBMDVO010000000
VERSION     DBMDVO010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24309_length_2164_cov_1.220553"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="ACZPE2_03450"
     CDS             <1..621
                     /locus_tag="ACZPE2_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1318937.1"
                     /translation="LSTGLSGVYNSACIARDELLEEYPDRKIYIVDSLGASSGYGLLM
                     DTLAELKNSGKSIEEVRDFAEERKLNVHHWFFSTDLTSYYRGGRITKTAQIFGTMLNI
                     CPLLNMDNNGKLIPRTKYRGKKKVIEEIVNRMVEHADGGLDYSGKCYISESACYDDAR
                     AVADLVESKFKKLNGKVEINSVGTVIGSHTGPGTVALFFFGDKRVD"
     gene            741..1412
                     /locus_tag="ACZPE2_03455"
     CDS             741..1412
                     /locus_tag="ACZPE2_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1318938.1"
                     /translation="MQKILIIEDDKVIARTLKEHLCKWDYDADFVVDFKNITEQVVSF
                     APQLILLDISLPYFNGFYWCGEIRKFSNVPIIFISSASDKMNIVMAVNMGGDDFISKP
                     FDLSVLTAKVQAVMRRTYSFSGTTSVIEHGGAILNLNDATIDFNGKKTELTKNEFKIL
                     QLLMENSGKVVPRDMIMDKLWESDSFIDDNTLTVNMTRLRKKLEDAGLCDFIVTKKGL
                     GYIVR"
     gene            1418..>2164
                     /locus_tag="ACZPE2_03460"
     CDS             1418..>2164
                     /locus_tag="ACZPE2_03460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1318939.1"
                     /translation="MKDWFKALFGYTAGNILSVLSFVFAAAVFYTVFWLYGVETEPVL
                     YATLISAVILLIVFVLRFSSVYKKHKRLEKIKNDAEYAVLKLPDYEDPIKKDLADIAE
                     KMSALKTDAETRMNRKYSDSADYFTLWAHQIKTPIAAMRLLLQTDYSSENDELEEQLF
                     KIEQYVEMVLQYIRSDSISSDLEIKSYDLGGIVRAAVKKYRKQFLRKKTKIVVGNVDC
                     KVLTDEKWLSFVIEQLISNALKYTSEGEINI"
BASE COUNT          631 a          434 c          505 g          594 t
ORIGIN      
        1 ctttcaaccg gactttcggg cgtatataat tccgcctgca ttgcgcgtga cgagctcctc
       61 gaagagtacc ccgacagaaa aatatatata gtagactctc tcggtgcttc ctccggctac
      121 ggtcttttaa tggacacttt ggcagagctt aaaaacagcg gcaagagcat agaagaggtt
      181 cgtgattttg ccgaggagcg taagctcaat gttcatcatt ggttcttttc taccgacctt
      241 acaagctatt atcgcggcgg cagaataaca aagaccgcgc agattttcgg cacaatgctc
      301 aacatatgtc ctctcctcaa tatggataat aatggcaagc ttataccgag aacaaagtac
      361 cgcggaaaga aaaaggttat tgaagagata gttaaccgta tggtcgagca cgcagacggc
      421 ggtcttgatt acagcggcaa gtgctacatt tccgaatccg cctgctatga tgacgcaaga
      481 gccgttgccg accttgttga gtcaaaattc aaaaagctca acggcaaggt cgaaatcaac
      541 agcgttggca ccgttatcgg ctcacatacg ggccccggta ccgttgcgct cttcttcttt
      601 ggcgataaga gagtagactg atataattta accgctcatt tcggcacagt atagggcagt
      661 attgtcttat accgcgccga tttttgtttt atatcgacaa gttgccgtat tcgtgttaaa
      721 atacatatga aggtgaaact atgcaaaaga tacttataat tgaagacgat aaggtcatag
      781 cgagaaccct taaagagcat ctttgcaaat gggattatga tgcggatttt gttgttgact
      841 ttaaaaatat aaccgaacag gtagtgtcat ttgcgccgca gcttatactc cttgatatat
      901 ctctgccgta ttttaacgga ttttactggt gcggcgaaat acgcaagttt tcaaatgtgc
      961 ctataatatt tatttcctcg gcaagcgaca aaatgaatat agttatggct gttaatatgg
     1021 gcggtgacga ttttatatcc aaaccgttcg atttgagcgt tctcactgca aaggtgcagg
     1081 cggtaatgcg ccgcacgtat tctttttcgg ggacaacgtc ggttatcgaa cacggcggcg
     1141 caatactcaa cctcaatgac gcgacgatag attttaacgg caaaaaaacc gagcttacga
     1201 aaaatgagtt taaaattttg cagctgctta tggagaattc gggcaaggtt gtcccgcgcg
     1261 atatgattat ggataagctt tgggaaagcg acagctttat tgacgacaac acactcacgg
     1321 taaatatgac gcggcttcgc aaaaagcttg aagatgccgg gctttgcgac tttatagtta
     1381 ccaaaaaggg tctcggttat atagtcaggt aggttttatg aaagattggt ttaaagcact
     1441 tttcggatat acggcgggca atatcttgtc cgtattatcg tttgtatttg cagcggcggt
     1501 attttatacg gtgttctggc tttacggagt ggaaacggag ccggtgctgt atgcgacgct
     1561 catctccgcc gtaattctat tgattgtatt tgttctgcgt ttttcctcgg tatacaaaaa
     1621 gcataagaga cttgaaaaaa ttaaaaacga cgcagagtat gccgttctta aattgccgga
     1681 ttatgaggac ccaataaaaa aggaccttgc cgatattgcc gaaaagatgt ccgcactcaa
     1741 aacagatgcc gaaacacgta tgaatcgcaa atacagcgat tcggcggact attttactct
     1801 ctgggcgcat cagataaaaa ctcccatagc cgctatgagg ctgctgctgc aaacagacta
     1861 ttcttcggaa aacgacgagc ttgaagagca gctttttaaa atagagcaat atgttgaaat
     1921 ggtgcttcag tacatccgtt ccgacagcat ctcttccgac ctcgaaatca aaagctacga
     1981 tttaggcgga atagttagag cggcggttaa aaaatacaga aagcagtttt tgcgtaaaaa
     2041 aacaaagatt gttgtgggca atgttgattg caaggtgttg acggatgaaa aatggttgtc
     2101 gtttgtaatt gaacagctta tttcaaatgc gttgaaatat acgtccgagg gtgaaatcaa
     2161 tatt
//
LOCUS       DBMDVO010000182         2161 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24363_length_2161_cov_1.150971, whole genome shotgun sequence.
ACCESSION   DBMDVO010000182 DBMDVO010000000
VERSION     DBMDVO010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24363_length_2161_cov_1.150971"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="ACZPE2_03465"
     CDS             <1..1486
                     /locus_tag="ACZPE2_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1318940.1"
                     /translation="INPDIPLGLEQITLRAMQKNPKDRYHSAAEMLVDLDAFRRNPSI
                     KFDYDYFVDNEPTKFVDSKAVNGTAARQPVPAERRPAAPAVNGDDDKNDDEKPANKTV
                     PILIGIIAGLVVIAAIVTGILLGRNVKKVEVPNFVGKNYIDDIMSNAEYNSNFKFETE
                     SVYSTDAEPGSVIKQTPDAGTKLRKGKTVKLQIAYNDDSVMIEGIIGLSSDDAEQKLK
                     KAGFTTKIITSYQKNVTPGLVFDSYPTEGSYAEKGSLVTLYVSTDKNSNSVKVPSVEG
                     YSADVAKQLIEAAGLKAKVTETDSDKPKGTVINQNPVGDSEADSGSTVEIVISSGTPA
                     STTASVTVNLPNLSGSSTGTVKIYLNSDLYATYTDVLLNGGTKTYEISGKGANNKYTI
                     FINDQNVQEGKIDFTQTPAKVSDVKNGSYDDKVALPNVVGKDVSEAKKTLETSGFTNV
                     KVVDENGNSASSGKVESMTPSGGGKVSPSTEIKLVVKSATPDNG"
     gene            1489..>2161
                     /gene="rsgA"
                     /locus_tag="ACZPE2_03470"
     CDS             1489..>2161
                     /gene="rsgA"
                     /locus_tag="ACZPE2_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1318941.1"
                     /translation="MSKTLNGKIIKGIGGFYYVEAANQVYECKARGVFRKEKTTPLCG
                     DVVTISVNDGAENTIDEIHPRKNQLMRPPVANIDSLIIVASTVKPRPSTLVIDKLTAV
                     AEHKNIEPVIIVTKDDLAPADELVSIYKHAGFASVAVSNETGEGVGEVRKLLCGKTSA
                     FTGNSGVGKTSLLNRIAPELKLATSAISEKLGRGRHTTREAELFSAAGGYIADTPGFS
                     SLELAM"
BASE COUNT          660 a          490 c          510 g          501 t
ORIGIN      
        1 gataaatccc gatattcctc tcggccttga gcagattaca ttgcgcgcca tgcagaaaaa
       61 tcccaaggac cgctaccatt cggcagccga aatgcttgtt gaccttgacg cattccgtcg
      121 caatccgtca ataaagttcg attatgatta ctttgtcgat aacgagccta caaagtttgt
      181 tgattcaaaa gcggtaaacg gcacagccgc aaggcagcct gttcccgcag aacgaagacc
      241 ggcagctcct gccgtaaacg gcgacgatga taaaaacgat gatgaaaagc cggccaataa
      301 gaccgttcct atacttatcg gcataatagc aggtcttgtt gttattgccg caatagttac
      361 cggcatactt ctcggccgca acgtcaaaaa ggttgaggtt ccgaatttcg taggtaaaaa
      421 ttatatcgac gatataatgt caaatgccga atacaacagt aacttcaagt ttgagaccga
      481 gtcggtatat agcaccgatg cagagcccgg ctccgtaata aaacagactc cggatgcagg
      541 aacaaagctt cgtaagggca aaaccgtaaa gcttcaaata gcatataacg acgacagcgt
      601 tatgatagaa ggcattatag gcctttcgtc tgatgatgcc gagcaaaagc ttaaaaaagc
      661 cggctttacc accaagataa taacgagtta tcagaaaaat gttaccccgg gacttgtgtt
      721 cgattcatat cccacggagg gctcttatgc cgaaaaaggc tctcttgtaa cgctctatgt
      781 ttcaaccgat aaaaattcaa attccgtaaa agtcccgtct gttgagggat acagtgcaga
      841 cgtcgcaaag caactcattg aggcggcagg tcttaaagct aaagttaccg aaaccgacag
      901 cgacaagccg aagggcacgg taataaatca gaatccggta ggagattccg aggcggattc
      961 cggctctacc gttgaaatag ttataagcag cggcactccc gcgagtacaa ctgccagtgt
     1021 aaccgtaaat cttcccaatc tctctggcag tagcacaggt accgttaaga tatatctcaa
     1081 cagtgacctt tatgctacct ataccgatgt gcttttaaac ggcggcacaa agacatacga
     1141 gatttccggt aagggcgcaa acaataaata caccatattc attaacgacc aaaacgttca
     1201 ggagggcaag attgacttta cccagactcc ggctaaggta agcgatgtta aaaacggctc
     1261 ttatgacgat aaggttgctc ttcccaacgt agtcggcaaa gatgtttcgg aggctaaaaa
     1321 gacgctcgaa acatcaggat ttacaaatgt aaaggttgtc gatgaaaacg gaaattcagc
     1381 ttcttcaggc aaggttgaga gtatgactcc gagtggcggc ggaaaagtat ctccgtctac
     1441 ggaaattaaa ctggtcgtta aatccgcaac gcccgataac ggatgagtat gagtaaaaca
     1501 ttaaacggta aaattataaa aggtattggc ggcttctatt atgttgaagc cgccaatcaa
     1561 gtatatgaat gtaaggcgcg cggagttttt cgcaaggaaa aaacaacacc gctttgcgga
     1621 gatgttgtca ccatatccgt aaacgacgga gctgaaaaca caatagacga gattcatccg
     1681 cgcaaaaatc agcttatgcg tcctcccgtt gcgaatatag acagccttat aattgtggcg
     1741 tcaactgtta agccgcgccc gtcaacttta gttattgaca agctgacggc cgttgccgag
     1801 cataaaaaca tagagcctgt aataatcgtg acaaaggatg atttggcacc tgccgatgag
     1861 cttgtgtcta tttataagca tgcgggcttt gcctctgttg ccgtttcaaa cgaaacaggc
     1921 gagggagtcg gtgaagtcag aaaactgctt tgcggaaaaa caagcgcttt tacagggaat
     1981 tcgggagtcg gaaaaacttc tcttttaaac aggattgctc ccgaactgaa gcttgccacc
     2041 tccgcgataa gcgagaagct cggcagaggc cgacacacta cgcgtgaagc ggagcttttt
     2101 tcggcggcag gcggatatat agcggataca ccgggctttt cttcgcttga gcttgcaatg
     2161 a
//
LOCUS       DBMDVO010000183         2157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24426_length_2157_cov_1.345817, whole genome shotgun sequence.
ACCESSION   DBMDVO010000183 DBMDVO010000000
VERSION     DBMDVO010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24426_length_2157_cov_1.345817"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="ACZPE2_03475"
     CDS             complement(<1..398)
                     /locus_tag="ACZPE2_03475"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1318942.1"
                     /translation="MLNTRAGGVLMHITSLPSKYGIGVMGEETKRFIRRIKDMGFSYW
                     QVLPLCPTDFYGSPYASPAAFALSPLLIDPDTLKNDGYVSEDDLARSVYYGSPYTADY
                     EFAAKSRRELLKIAYQNKKNEVSAETEKFC"
     gene            complement(433..1305)
                     /locus_tag="ACZPE2_03480"
     CDS             complement(433..1305)
                     /locus_tag="ACZPE2_03480"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1318943.1"
                     /translation="MKKKVRLKKTVKRKFLLFVILAVIMTGLIVGELTIGQREVNDPA
                     VERLEAKISETTTQEEPQEETTEEETTAETTAPETVPETTASAPPVTASAADFNNPQT
                     VDPNSGNWELTLVNAGHKLPDGYVPSLANAISGSSVQLDSRVVKAYQEMYDAAKKDGC
                     VLTPYAGYCSVSRQNDNYNRKVSYYKNQGLSDDDAAAKARTFILPGGYSEQNLGLSMD
                     IVSASSDFASTKEFSWLVKNAQDYGFILRYPENKTDKTGMNYQPWHWRYVGKEAAKAM
                     NKSGLCLEEYLKAA"
     gene            complement(1569..>2157)
                     /locus_tag="ACZPE2_03485"
     CDS             complement(1569..>2157)
                     /locus_tag="ACZPE2_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1318944.1"
                     /translation="AVIRAAINVSKKHPDWKIVPEIMIPLVGEIKELKYVKNIVVETA
                     DAEIAAAGSDMKYEVGTMIEIPRAALTAGDIAKEAEFFCFGTNDLTQMTYGFSRDDAG
                     KFLGAYYDKKIFESDPFAKLDQVGVGQLMEMAVKNGKATRPSLHCGICGEHGGDPSSV
                     EFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEKNK"
BASE COUNT          513 a          559 c          468 g          617 t
ORIGIN      
        1 tcacagaatt tttcagtctc cgcagatact tcgtttttct tgttttgata cgcgattttc
       61 aaaagctccc tgcggctttt tgcggcaaat tcgtagtccg ccgtgtaagg cgagccgtaa
      121 tatacgcttc gtgcaaggtc gtcttccgac acatagccat catttttgag cgtgtccggg
      181 tcaatcagaa gcggtgacaa cgcaaatgcc gccggagacg cataaggtga gccgtaaaaa
      241 tccgtaggac aaagcggcag cacctgccaa tatgaaaacc ccatatcctt gatacggcgt
      301 ataaaccgtt ttgtctcttc gcccattacg ccaattccgt attttgacgg gagcgacgtg
      361 atatgcatta aaactccgcc ggctcttgtg ttaagcatta tataattccc ctttttttag
      421 cctcgtcttg atttatgccg ctttgagata ctcctcaagg cacaggccgc tcttattcat
      481 cgcctttgca gcctctttgc cgacataacg ccaatgccac ggctgataat tcatacccgt
      541 tttgtcagtt ttattttcgg gatagcgcaa aataaagccg taatcctgcg cattttttac
      601 aagccacgaa aactcctttg tagaggcaaa atcggagctt gcactcacaa tatccattga
      661 cagaccgaga ttttgctcgg aatatccgcc cggcaaaata aacgtgcgcg cctttgcggc
      721 ggcgtcgtca tcggacaagc cctgattttt ataataactc acctttcggt tgtaattatc
      781 gttttgacgt gaaaccgagc aataccctgc atacggagtt aaaacgcagc cgtccttctt
      841 tgcggcgtcg tacatctctt gatatgcttt aacaactctc gaatcaagct gcacgctgct
      901 tccgcttatc gcgtttgcaa gcgacggcac atatccgtcg ggcagcttat gccctgcgtt
      961 aacaagcgtg agctcccaat tgcccgagtt cgggtctacg gtttgaggat tgttaaaatc
     1021 ggctgccgaa gccgtaacgg gcggtgccga tgcggtcgtc tccggaacgg tttcaggggc
     1081 tgtcgtttcc gcggtagtct cttcttcggt cgtctcctcc tgcggttctt cctgtgtggt
     1141 tgtttcgctg atttttgctt cgagcctctc aactgcaggg tcatttacct ctctttgccc
     1201 tatcgtaagc tcgccgacta taagccccgt cattataacg gccaaaatta caaacagcaa
     1261 aaattttctt tttaccgtct ttttcaatct gacttttttc ttcatatttc gcgtcctttt
     1321 aaaatctctt cttgatattt taaaatgttt acgtcggatt gtcaatcaaa aggcgcattt
     1381 cggcgacaaa attcagccga cggagacttt tttctcatca aagtaaacga agccgtcaat
     1441 ccacacattg atttttttat ctttaaggcg ctccgacaat gcgcaaaaaa gaccctgcgg
     1501 attttactct gcaaggtctt tttcctgtta tggtttaaaa attggggtta cccaaaatca
     1561 agctctgatt atttgttctt ctctgcgatt gcagcctgag cagcagcaag acgagcgatc
     1621 ggaacacgga acggtgagca agatacatag tcgaggccga tcttgtggca gaactcaaca
     1681 gaagacgggt cgccgccgtg ctcaccacag ataccgcagt gaagtgacgg acgggtagct
     1741 ttaccgttct taacagccat ttccatgagc tggcctacgc cgacctggtc aagcttagca
     1801 aacggatcgc tctcgaatat cttcttgtca tagtaagcac cgaggaactt accagcatcg
     1861 tcacggctga agccgtaagt catctgagta aggtcgttgg taccgaagca gaagaactca
     1921 gcttccttag cgatgtcgcc tgcggtaaga gccgcacgcg gaatctctat catggtacct
     1981 acttcatact tcatatcgga gccggcagct gcaatttctg catctgcagt ctcaacaaca
     2041 atattcttta catatttcag ctctttgatc tctcctacca acggaatcat gatctccgga
     2101 acgatcttcc aatccggatg tttcttggat acgttgatag ctgcacggat aacagct
//
LOCUS       DBMDVO010000184         2151 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24521_length_2151_cov_1.668780, whole genome shotgun sequence.
ACCESSION   DBMDVO010000184 DBMDVO010000000
VERSION     DBMDVO010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24521_length_2151_cov_1.668780"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="ACZPE2_03490"
     CDS             complement(<1..232)
                     /locus_tag="ACZPE2_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1318945.1"
                     /translation="MKAAVIGAGLAGCEAAKQLSRAGIPTYLYEMKPKKFSPAHKNEN
                     FAELVCSNSLKAERLNSAAGLLKAEMEMLSSVC"
     gene            complement(232..>2151)
                     /gene="topA"
                     /locus_tag="ACZPE2_03495"
     CDS             complement(232..>2151)
                     /gene="topA"
                     /locus_tag="ACZPE2_03495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1318946.1"
                     /translation="NADEVLLATDPDREGEAISWHLATVLGLPLDEKNRVKFNEINEN
                     AVKKGIASPEKIDMDLVDAQQARRILDRLVGYKLSPFVSQKIHRGLSAGRVQSVAVRL
                     VVDREEEIRKFVPEEYWSLDAKFTAPSSKKAFKAAFTGDETGKIKLKTKEETDAILAR
                     LQDAEYSVSSVKKGTRRRSPAPPFTTSTMQQDASRKLGFQAKRTMKIAQELYEGMEVE
                     GFGATGLITYMRTDSLRISEEARREGNAFIEENYGKQYLPEKPRYFKTKASAQDGHEA
                     IRPTVPAITPEIAKKSLTPEQFKLYSLIWKRFMASLMSNCIQDTVKIEIKAVGESDKN
                     TDRYCTFNASGYTVRFDGYTRLYEAATDEDEDEGKLPEIKAGDALKLKEMLGNQHFTQ
                     PPARYTEGSLIKMLEETGVGRPSTYASIVSTITSREYVVREQKQLKPTELGEAVVKLL
                     KEHFPNIVNVKFTASMESDLDKVEHHEIDYIAMLHTFYDGFDKTLEKAKADMEGVKIK
                     LKEDETNIPCEKCGRMMVIKTGRFGKFLACPGYPECKNTKPYVIETTATCPVCGGKVI
                     QKKSKKGYTFFGCENYPECNFMTWDKPTDEKCPKCGKSLFKKKGGILACLDENCGFEK
                     KAERKKKSAKTQEED"
BASE COUNT          425 a          579 c          491 g          656 t
ORIGIN      
        1 cgcacacgga agaaagcatc tccatctccg cctttaaaag ccctgccgcg gaattgagcc
       61 gctccgcctt cagtgaattg gagcatacaa gctccgcaaa attttcattt ttatgcgccg
      121 gagaaaattt cttcggcttc atttcataga gatatgtggg tattcccgcc ctcgacagct
      181 gctttgccgc ctcacatccg gcgagacccg caccgattac cgctgctttc atcagtcttc
      241 ctcttgagtc tttgcgcttt tctttttgcg ctccgctttt ttctcaaaac cgcagttttc
      301 gtcaaggcaa gctaagattc cgcctttttt cttgaagagc gacttgccgc acttgggaca
      361 tttttcgtcc gtcggcttat cccatgtcat aaagttgcat tcgggataat tttcgcagcc
      421 gaagaacgta tatccttttt tgctcttttt ctgaattacc ttgcctccgc acaccgggca
      481 ggtggcagtt gtctctatta cgtacggctt tgtgtttttg cactccggat agcccgggca
      541 cgcgaggaat ttaccgaatc tgccggtttt tataaccatc attcttccgc acttttcgca
      601 cggaatattc gtctcgtcct ccttaagctt tatcttaacg ccctccatat cggcttttgc
      661 cttttcaagc gttttgtcaa agccgtcata aaacgtatga agcattgcta tataatctat
      721 ttcgtgatgc tctaccttat cgagatcgct ctccatggag gccgtaaatt ttacgttaac
      781 tatattcggg aaatgctcct ttaagagctt gacgacagct tctccgagct ccgtgggctt
      841 aagctgcttc tgctcgcgga caacatattc gcgcgaggta atggttgaca ctattgaggc
      901 gtacgtactc ggtctgccga cgcccgtttc ctcaagcatc ttaatgagcg agccctcggt
      961 atagcgcgca ggcggctgtg tgaagtgctg atttccgagc atttccttga gctttaatgc
     1021 atcgcccgcc ttgatttcgg ggagcttgcc ctcgtcctcg tcctcatctg ttgcggcttc
     1081 gtaaaggcgt gtataaccgt caaaacgaac cgtataaccg ctcgcattaa acgtgcaata
     1141 tctgtcggtg tttttgtcac tctcgccgac tgcctttatt tctatcttta ccgtatcctg
     1201 tatgcaattt gacataagac ttgccataaa gcgcttccaa ataaggctgt aaagcttgaa
     1261 ctgctccgga gtaagactct ttttggcaat ttcgggtgtg attgccggaa cggtggggcg
     1321 aattgcctcg tggccgtcct gtgcgctcgc cttagtttta aaataacgcg gcttttcggg
     1381 caaatactgc ttgccgtaat tctcctcgat aaaggcgttg ccctctcgcc ttgcttcttc
     1441 cgatatacgg agcgagtcgg ttctcatata tgttataaga cctgttgcgc cgaagccctc
     1501 tacctccata ccttcataaa gctcctgcgc tattttcata gtgcgcttag cctgaaatcc
     1561 gagctttctg gaggcgtcct gctgcatggt ggaggttgta aacggcggcg ccggagaacg
     1621 tctgcgcgtt ccttttttga ccgacgacac ggagtattcc gcatcctgaa gtcttgccaa
     1681 aatcgcgtcg gtctcctctt ttgttttgag ctttatcttg ccggtttcgt cgcccgtaaa
     1741 cgctgcctta aacgccttct ttgaggacgg cgcggtgaat tttgcgtcaa gtgaccaata
     1801 ttcttcggga acgaatttgc ggatttcctc ttcgcggtca acgacgagcc ttacggcaac
     1861 ggactgaact ctgcctgccg aaagtcctct gtgtatcttt tgcgatacga acgggctgag
     1921 cttatagccg acgagtctgt cgagaattct tctcgcctgc tgagcgtcaa caaggtccat
     1981 atcgattttt tcggggcttg ctatgccctt tttgaccgcg ttttcgttta tctcgttaaa
     2041 tttaactctg ttcttttcat caagcggcag cccgagaacc gtggcaagat gccaggaaat
     2101 agcctctccc tctcggtcgg ggtcggttgc gagcagaacc tcatctgcat t
//
LOCUS       DBMDVO010000185         2134 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24813_length_2134_cov_1.023610, whole genome shotgun sequence.
ACCESSION   DBMDVO010000185 DBMDVO010000000
VERSION     DBMDVO010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24813_length_2134_cov_1.023610"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="ACZPE2_03500"
     CDS             complement(<1..340)
                     /locus_tag="ACZPE2_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1318947.1"
                     /translation="MNKKVIVVGAGASGLMAAGTAAKNGNDVILFERNDKVGRKIMIT
                     GKGRCNVTNATFDTKSLIDNVPRNGRFLYSAFSEFSARDTMDFFEELGVPLKVERGNR
                     VFPVSDKAVDI"
     gene            complement(333..1211)
                     /locus_tag="ACZPE2_03505"
     CDS             complement(333..1211)
                     /locus_tag="ACZPE2_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1318948.1"
                     /translation="MKGSFLSKLDSNIRKLSKGHKKIASYIKENYDKAAFMTASALGR
                     KVGVSESTVVRFATELGFKGYPELQKELQQMIKSKLTAVQRMEVSESLIGDNDIVRTV
                     LNGDIDLIRETYEKTSREDFAKAVSTINKARKIYILGVRSSAALASFLGFYFNLVFDS
                     VELIDTSSASEMFEQMFRIDKEDVCIAISFPRYSKQTVNAIRFVSDRGAKIVAITDSD
                     TSPIASYADCLLVARSDMASVVDSLVAPLSLINALIVAVTLSKREEVINNFNKLESIW
                     DEYQVYEKAEDISENE"
     gene            complement(1323..>2134)
                     /locus_tag="ACZPE2_03510"
     CDS             complement(1323..>2134)
                     /locus_tag="ACZPE2_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1318949.1"
                     /translation="ELKNGVSYPAARFAAVRDVFGAETAAVLSEPNNILAVEYIKALK
                     ALSSDMKPHTVKRTGASHDSDEYVGNIASASKIRELIRSNANTGLFLPSDSQAVLTNE
                     IAIGAAPSDMNRIGNAVIASLKLKTPSDFNNIYGVGEGIENRLLSAAASARNLTELYD
                     FAKTKRFTHSRIRRVVLNSFFGITNDITLSDPPYIRVLGFTHKGKDILRIARKNASLP
                     IVMTVGDVKKSGGLSERLYSLECRTTDIFNMTLPKIRQSGTDMTDNLVRIE"
BASE COUNT          575 a          500 c          421 g          638 t
ORIGIN      
        1 ctatatccac cgctttatcc gaaaccggga agactctgtt tcctctttca actttaagag
       61 gcaccccaag ctcttcgaag aaatccattg tatctcttgc ggaaaactca gaaaaagcgc
      121 tgtataaaaa tctgccgttc cgcggaacat tatcaatgag acttttagta tcaaaggtgg
      181 catttgtgac gttacaacgt cctttaccgg ttatcattat ttttctgccg accttatcgt
      241 ttcgttcaaa aagaattacg tcgttaccgt tttttgccgc tgtgccggca gccataagtc
      301 ctgaggctcc ggctcctaca actataactt ttttattcat tttcggaaat atcctctgct
      361 ttttcgtata cctggtattc gtcccaaata ctctcgagct tattgaaatt gttgattacc
      421 tcttcacgct tactcagcgt tacagcaaca ataagtgcat ttataagact aagcggtgca
      481 acaagagaat caactacgga tgccatatcg gagcgcgcta caagcaggca atcggcatat
      541 gacgctatgg gtgacgtatc cgaatcggtt atcgccacta ttttggcacc tctgtccgat
      601 acaaatctta ttgcatttac agtctgcttt gaatacctcg gaaagcttat ggctatgcac
      661 acgtcctctt tatctattct gaacatctgt tcaaacattt cggacgcgct tgatgtatct
      721 ataagctcaa ccgaatcaaa gacaagatta aaataaaaac cgagaaaact tgcaagtgcc
      781 gcagaagaac ggacgccgag aatatatatt ttgcgagcct tatttatagt agatacggct
      841 tttgcaaaat cctcccttga tgtcttctcg taggtttcgc gaatcaaatc aatatccccg
      901 ttaagaacgg ttctgacaat atcgttgtca cctataaggc tttccgaaac ctccattctt
      961 tgaactgcgg tcagctttga ttttatcatc tgctgaagct ctttttgaag ctccggatag
     1021 cccttaaatc caagctccgt tgcaaaccgc acgacggtac tctcgctgac cccgactttt
     1081 cggccgaggg cagaagctgt cataaatgcg gctttatcat agttttcttt aatatacgag
     1141 gcgatcttct tatgcccctt agagagcttt ctaatatttg aatcaagctt tgaaagaaaa
     1201 gaacccttca atgtacagcg cttccttgtc ttacataata atgccgcatt cgatgcagca
     1261 acactattat tataatgtct attatacaaa aatcaagcaa aaatgcagga tttgctgcaa
     1321 aattattcaa tcctgactaa attatccgtc atatcagttc cgctttgcct aattttaggc
     1381 aatgtcatat tgaaaatatc cgtagttctg cattcaaggg agtataatct ttcagaaaga
     1441 cctcctgact ttttgacgtc gccgacagtc ataacaatcg gtaaggatgc gtttttcctc
     1501 gcaattcgca atatgtcttt gcccttgtgt gtaaatccga gcacacgaat atacggaggg
     1561 tctgacaagg tgatatcgtt tgttataccg aaaaacgaat ttagaacgac gcgccgaata
     1621 cgcgaatgag taaagcgctt tgtttttgca aaatcataaa gctccgttag gtttcgcgcg
     1681 gatgcggctg ctgagagcag acggttttct attccctcgc cgacgccgta aatattatta
     1741 aaatcagacg gagttttaag ctttaaggac gctattacgg catttcctat tctgttcata
     1801 tcggacggtg ccgctccaat tgcgatttcg ttagttaaaa cagcttgaga gtcagacggc
     1861 aaaaacaagc cggtattcgc gttgctgcgt atcagctctc tgattttaga agcggaggcg
     1921 atattaccga cgtattcgtc cgaatcatgc gacgcaccgg ttcgctttac ggtatgaggt
     1981 ttcatatccg aagataacgc tttaagcgct tttatatact ctaccgccaa aatgttgttg
     2041 ggctccgaaa ggactgcggc tgtttccgct ccgaacacgt ctcttaccgc ggcaaacctc
     2101 gccgccggat agcttacacc gtttttaagt tcgg
//
LOCUS       DBMDVO010000186         2128 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24913_length_2128_cov_1.103108, whole genome shotgun sequence.
ACCESSION   DBMDVO010000186 DBMDVO010000000
VERSION     DBMDVO010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24913_length_2128_cov_1.103108"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="ACZPE2_03515"
     CDS             complement(<1..1599)
                     /locus_tag="ACZPE2_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR1318950.1"
                     /translation="MNGFLPVTKEEMIALGWDSPDFVYVSGDAYVDHPSFGAAIITRV
                     LASQGFRVCMLSQPNWKNTCDFMRFGRPKYGFLLSSGNIDSMVAHYTVAKKPRSKDAY
                     SPGGKMGKRPDRAVIVYCKKIREAYNNIPIIIGGLEASLRRFAHYDYWEDKLRPSILF
                     DSGADLLIYGMGEKQTLEVANRLKNGEDIHLMHDINGTCYAVPVSETPLYGKECPSYE
                     NVLKSKKEYAVSVRIEQDEQDHIRGKLLKQRHGNMMLVQNPPMSPLTRDELDFVYSLP
                     YTREYHPMYEEFGGVPGLEEVKFSITHNRGCFGACNFCSLAFHQGRFVTSRSKTSVLA
                     EAEKLKQLPDFKGYIHDVGGPTANFRYPSCEKQLKSGLCKGKKCLAPSACPALKADET
                     EYLDILRELRRIPGIKKVFIRSGIRYDLMLKDKNNDFFKELVKYHISGQLKVAPEHCS
                     AAVLDKMGKPHIDAYIEFSKKYFDYTKRIGKEQYLVPYLMSSHPGATLKDAVKLAEFI
                     KKEHLHPEQVQDYYPTPGTISTAMF"
     gene            complement(1603..>2128)
                     /locus_tag="ACZPE2_03520"
     CDS             complement(1603..>2128)
                     /locus_tag="ACZPE2_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1318951.1"
                     /translation="AEKLLGNGDLLFYPVGIAKPQRIQGCFLSDKEVETVVDFIKKQE
                     KSSYDDEVMNEIERQAAMEKKKNGGAVSDGDDSDGETDEMVPKAIEVVVDAQMASTTL
                     LQRKLKLGYARAARIIDTLEERNIIGPYEGSKPRKVLVTKQQWYEMNALAQGGAAKDY
                     TASDEKNDDTEIPE"
BASE COUNT          577 a          502 c          392 g          657 t
ORIGIN      
        1 aaacattgca gttgaaattg ttcccggagt gggataataa tcctgcacct gctctgggtg
       61 taaatgttct ttttttataa attccgcaag cttaaccgca tcctttagag tggctcccgg
      121 atgtgaagac attagatatg gaacaagata ctgttccttc ccaatacgct tagtataatc
      181 aaaatacttt tttgaaaatt ctatgtaagc gtctatatgc ggtttaccca ttttgtcgag
      241 aactgcagcg gagcaatgct ccggtgcaac ctttagctgc ccgcttatat gatattttac
      301 aagttctttg aaaaaatcat tatttttatc ctttagcata agatcatatc ttataccgga
      361 gcgaataaaa acttttttga tgcccggaat ccttctcagc tctcttaaaa tatctaaata
      421 ttcagtttca tccgctttaa gagcgggaca agcactcgga gccaagcatt ttttaccttt
      481 acacaagccg ctttttaact gtttttcaca cgaaggatac ctaaagtttg cggttgggcc
      541 tcctacatca tgtatatacc ctttaaaatc aggtaattgt ttcagttttt cagcctcggc
      601 gagaacggat gttttgcttc ttgatgtaac aaaccgcccc tggtgaaatg ctaatgagca
      661 aaagttacac gcgccgaaac agccgcggtt gtgtgttatc gaaaacttaa cttcttcgag
      721 ccccggaact ccgccaaatt cttcatacat agggtgatac tcacgagtat aaggaagaga
      781 ataaacgaag tcaagctcat ctcttgttaa cggagacatc ggcggattct gcacaagcat
      841 catattaccg tgacgctgct tcagaagctt tccgcgaata tgatcctgct catcctgctc
      901 aattctcacg gaaacagcat attccttttt ggatttcaat acattctcgt aagatggaca
      961 ttctttaccg tacagcggag tttcggaaac aggaactgca tagcacgttc cgttaatgtc
     1021 atgcattaga tgtatatctt caccgttttt cagcctgttc gccacttcaa gcgtttgctt
     1081 ttctcccatt ccgtaaataa gtaaatcagc ccccgagtca aaaagaatcg agggtcgtag
     1141 cttatcttcc caataatcgt aatgagcaaa tctgcgaagc gaagcctcaa gcccgcctat
     1201 tattatagga atgttattat aagcttctct gattttttta caataaacga ttacggctct
     1261 atcaggtctt tttcccattt ttccgccggg agaatacgcg tccttacttc gaggcttttt
     1321 agctacggtg taatgagcaa ccatagaatc aatattgccg gaagagagca aaaagccata
     1381 cttcggcctg ccgaaacgca taaaatcgca tgtgtttttc caattaggct gcgacagcat
     1441 acacacccga aatccttgag atgccagcac acgggtaatt atagctgcac caaagctggg
     1501 atgatccaca taagcgtcac cggaaacata aacaaaatca gggctatccc atccgagcgc
     1561 aatcatttct tcttttgtta caggtaagaa accattcata atttactccg gaatttccgt
     1621 atcatcattt ttttcatcgg aggcagtgta atcttttgca gctccaccct gtgccaaagc
     1681 attcatctca taccactgtt gctttgttac gaggactttt ctcggtttac tgccttcata
     1741 cggacctata atatttcgct cttcaagagt atctataatt cttgcggctc ttgcataacc
     1801 gagcttaagt tttctttgaa gcagggttgt ggaagccatc tgcgcatcaa cgacaacctc
     1861 tattgctttc ggaaccattt cgtctgtttc gccgtcggaa tcatcaccgt ccgaaacagc
     1921 tccgccgttt ttcttctttt ccattgcagc ctgtcgctcg atttcattca ttacttcatc
     1981 gtcataagaa gatttctcct gctttttaat gaagtcaaca acagtttcta cttctttatc
     2041 cgaaagaaaa caaccctgta tacgttgagg ctttgcaatt cctaccgggt agaataaaag
     2101 gtctccgtta cctaaaagct tttcagct
//
LOCUS       DBMDVO010000187         2124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_24976_length_2124_cov_1.187840, whole genome shotgun sequence.
ACCESSION   DBMDVO010000187 DBMDVO010000000
VERSION     DBMDVO010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24976_length_2124_cov_1.187840"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2124)
                     /locus_tag="ACZPE2_03525"
     CDS             complement(<1..>2124)
                     /locus_tag="ACZPE2_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014805.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1318952.1"
                     /translation="VGIHSGDSIVVIPTQTLTDSETVRLRRAARKIADYLNIEGSCNV
                     RFAINSDGSEYMVLAVEPQLNRTTALVSKATGYPIAQVSAKIALGYKLYEIKNDITGV
                     TTAANEPAIDYCAVKVPRWSFENFDAATRKLGGTMQATGEALAIGTSFELALMKAIRS
                     MNPRTESVSMPKMKQKTDNEIKELLLGSDDERIFAVYEAIRRGFDLAEIQSLTRIDNY
                     YLTKLKNIADTETSLGNGFSGDLYFRAKTLGFLDSTIEKITGEEISAPIAAGYNTVDT
                     CAAEFDVKKPYFYSSFDEDNEAAMFGKAHPTSKKKILVVGSGPTSIGLGTDRDYAVVN
                     CINTLKDFGYSTILLNNNPAAVSTDPGVADTLYLDPITDEDVRNVVLTEKPYGAVLPF
                     GGGNAVRKAEMLRSLGVKVFGSDDEAHRRLKNKIELFDILDDLGIRHTNNRHVMIGKG
                     AEVDVLSDGSDILVPGICEHIEKACVNPGDTTTVFPSITLTSSDKAKIYEYTEKLCKE
                     LKFKGLINIQFVIYDNEVYVSSASVVATRNVPFMTKASGLPIVAMATRLMLGETLGDI
                     GMGCGILPEPTRYFVRVPVFSFEKLQGTDVQIGDTEKSTGEVMGIADTFDEALLKGLI
                     ASGMRIKRTGGVLVSVADTDKRDSVMLAGEFLKQGFKIYATSNTAKLLNSNHVAANSV
                     RKIHEGEPNTMSLIKNNKLSYVVSTA"
BASE COUNT          565 a          504 c          444 g          611 t
ORIGIN      
        1 gcagttgaaa caacatatga taatttatta ttcttaataa gcgacattgt attcggctcg
       61 ccctcatgta ttttacgcac ggaatttgcc gcaacatggt tcgaatttaa aagcttagca
      121 gtattggatg tagcatatat cttaaagccc tgttttaaaa attcaccggc aagcataacg
      181 ctgtccctct tatcggtatc ggcaacggat acaagcactc cgcctgtgcg ctttatacgc
      241 attcccgaag ctatcaagcc cttcaaaaga gcttcgtcaa atgtatctgc tatgcccatg
      301 acttcacccg ttgacttttc ggtatcgcct atctgaacat cggtaccctg aagtttttcg
      361 aagctgaaca ccggtactct gacaaaatac cttgtaggct ccggtaaaat accacatccc
      421 ataccgatat caccgagcgt ctcgccgagc ataagccgcg tagccatggc aactatcggc
      481 aacccagatg ccttagtcat aaacggcacg tttcgcgttg ccacgacgct tgccgacgaa
      541 acataaactt cgttatcata tataacaaac tggatattta taagcccttt aaatttaagt
      601 tctttgcaaa gcttttcggt atattcgtat atctttgctt tatctgatga agtaagcgtt
      661 atcgacggaa aaaccgtagt agtgtcgccg ggatttacac aggccttttc aatatgctcg
      721 caaattcccg gaaccagtat atcgctgcca tcggacagga catccacctc ggcaccctta
      781 ccaatcataa cgtgtcggtt atttgtgtga cgtataccga gatcatcaag gatatcaaat
      841 aactcgattt tgttttttag tctgcggtga gcctcgtcat ccgaaccgaa aaccttaacg
      901 cctaatgaac gaagcatttc ggcttttctg acggcgttac caccgccgaa cggcaaaaca
      961 gctccgtacg gtttttcggt gagcacaaca tttctgacat cttcgtctgt aatggggtca
     1021 agataaagtg tatcggcaac tcccggatct gtggaaacag ccgcaggatt attattaagc
     1081 aaaatagtgg agtaaccgaa gtccttaagt gtatttatac agtttaccac cgcataatcg
     1141 cggtctgttc caagacctat ggatgtaggg ccggaaccca ctacgaggat tttctttttc
     1201 gatgtcgggt gcgcttttcc gaacattgcg gcttcgttat cctcgtcaaa cgaagaatag
     1261 aaatagggct ttttaacgtc aaattcggct gcgcaagtgt ctacggtgtt atatcctgca
     1321 gctatgggtg ccgaaatctc ctcaccggtt attttttcaa ttgtactgtc gagaaaaccg
     1381 agagtttttg cacggaaata caaatcaccc gaaaagccgt taccgagaga tgtttccgta
     1441 tcggctatat tcttgagctt agttagataa taattatcta ttcttgtaag agactgtatt
     1501 tcagcaaggt caaatccgcg tcttatagct tcgtaaacag caaaaattcg ctcatcatcg
     1561 gagcctaaca gcagctcctt tatctcattg tcggtttttt gcttcatttt tggcattgat
     1621 acagattcgg tacgcggatt catcgagcgt atcgctttca taagagccaa ctcaaacgaa
     1681 gtgccgattg cgagtgcctc acccgtagcc tgcatagttc cgccgagctt tctggtggcg
     1741 gcatcgaagt tttcaaaaga ccatctcgga acctttacgg cacaataatc aatggcaggt
     1801 tcatttgccg ctgtggtaac gcctgttata tcgtttttga tttcgtaaag tttatatccg
     1861 agagcaattt tggcagagac ctgcgctatg ggataacccg tagcctttga aacaagagcg
     1921 gtagtacggt taagctgtgg ctcaactgcc aaaaccatat actcgctgcc atccgaattt
     1981 attgcaaaac ggacattgca gcttccttca atatttaaat aatcggctat ttttcttgcg
     2041 gctctgcgaa gccttacggt ttcgctgtct gtaagagtct gcgtaggaat aacaacaata
     2101 ctgtcgccgg aatgaatgcc gacg
//
LOCUS       DBMDVO010000188         2120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25038_length_2120_cov_1.118375, whole genome shotgun sequence.
ACCESSION   DBMDVO010000188 DBMDVO010000000
VERSION     DBMDVO010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25038_length_2120_cov_1.118375"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="ACZPE2_03530"
     CDS             complement(<1..244)
                     /locus_tag="ACZPE2_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1318953.1"
                     /translation="MENTLTRKKQIITAKALTTVIGITLSVAIPQLFHVIGIVSGTGS
                     LPGSAFLPMHLGVFFTALLAGPAVGAVTGAVSPLLSF"
     gene            complement(269..808)
                     /locus_tag="ACZPE2_03535"
     CDS             complement(269..808)
                     /locus_tag="ACZPE2_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1318954.1"
                     /translation="MNKKVLIVSSSPRKHGNSDLLCDEFARGAKDAGNEVEKVFLADK
                     SINYCRGCGICNSTHKCVQKDDMAELNDKMVKADVIALASPVYFYTVDAQLKTFIDRC
                     VPRYTEMSDKDFYYILTAADSDKSAFDKSIECIRGFTLDCLDGAKEKGIIRGAGVFEK
                     GEIKDKSEYKECYNMGKNV"
     gene            1080..>2120
                     /gene="htpG"
                     /locus_tag="ACZPE2_03540"
     CDS             1080..>2120
                     /gene="htpG"
                     /locus_tag="ACZPE2_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1318955.1"
                     /translation="MRTKQFKAESKKLLDLMINSIYTHKEIFLRELISNASDAVDKLY
                     FRSLTDQNVGMSHGDFEIRITPDKENRTLTISDNGIGMTKEELEANLGTIAKSGSLAF
                     KQENADAKDIDVIGQFGVGFYSAFMVSDCVTVESRAYGESEAYRWQSKGVEGYTVEEC
                     DKPDVGTTITLHIKDDTDEEKYSQFLEEYTIKTIVKKYSDYVRYPIRMTVEKRRLKEG
                     TEDEYETVKEDETLNSMIPLWKKNKKEIKPEEYEAFYKDKFMDYEKPLKVIHYKTEGQ
                     ATYDALLFIPSHAPFDYYTKEYEKGLQLYSKGVLIMDKCKDLLPDHFSFVKGLVDSED
                     LSLNISREVLQHD"
BASE COUNT          605 a          434 c          465 g          616 t
ORIGIN      
        1 cgaagctcaa aaggggactt accgctcccg ttacggcacc caccgcggga cctgccaaaa
       61 gagctgtgaa gaatacgccg agatgcatcg gcagaaatgc cgagccgggt aagctgccgg
      121 taccgctcac gatacctata acgtggaaca gctgaggtat ggcaaccgac agagttattc
      181 ctattaccgt cgtgagagct ttagctgtta tgatttgctt ttttcttgtc aaggtgtttt
      241 ccataaatat cggtctcctt aaattgtttt aaacgttttt gcccatattg tagcattctt
      301 tgtattccga tttgtctttg atttcgcctt tttcaaatac accggcaccc ctgattattc
      361 ctttttcctt tgcaccgtca aggcagtcga gcgtaaagcc ccttatgcat tctatcgact
      421 tgtcaaatgc cgatttatcg gaatccgccg cggtcagaat ataatagaaa tccttgtccg
      481 acatctctgt gtaacgcggc acgcacctgt ctataaatgt tttaagctgt gcgtcaacag
      541 tatagaagta cacgggggaa gccaaagcta taacgtcggc tttgaccatt ttgtcattaa
      601 gctccgccat atcatccttt tgaacgcatt tatgcgtaga attacatatt ccgcagccgc
      661 ggcagtaatt tatgcttttg tctgcgagaa agaccttttc gacctcgttt cctgcgtctt
      721 ttgctccgcg tgcgaattca tcgcagagta aatccgagtt gccgtgcttt ctcggacttg
      781 atgaaacaat gagaaccttt ttattcattg cagtcagctc cttttgcagt atcgtcatag
      841 cccattgtgg gcgatgatgt taagtgtgct aattttaaat ataatttaac acttggagtt
      901 tactctaagt caagcctttt tttgaaaagt tttgcccaaa acaaaataat agaattttgt
      961 ctgttaatat gtaaactgtt tattaactct gtttgacttt tcttgacttt taattttgcg
     1021 gtgatataat attattactc tttgaaaaag ggctattttt atggaaacag gtgatttgta
     1081 tgcgtacaaa acagtttaaa gccgaatcaa agaagctgct tgatttaatg ataaactcca
     1141 tatacacgca taaagaaata tttttgcgag agcttatatc aaatgcaagt gatgccgttg
     1201 ataagctgta tttccgttcg ctgaccgacc aaaatgtcgg aatgtcgcac ggtgattttg
     1261 aaatcagaat tactccggat aaggaaaaca gaactctgac aatatcagat aacggtatag
     1321 gtatgaccaa ggaggagctt gaagcaaacc tcggcactat cgcaaagagc ggctctctcg
     1381 cttttaagca ggaaaacgcc gacgcaaagg atatagacgt tatcggtcaa ttcggcgtag
     1441 gcttttattc ggcatttatg gtgagcgact gtgtaaccgt tgaaagtcgc gcttacggcg
     1501 aaagcgaggc ttaccgctgg cagtctaagg gcgttgaggg ctataccgtt gaggaatgcg
     1561 acaagcccga cgtcggcact acgataactc tccatatcaa agatgatacg gatgaagaaa
     1621 agtattctca gttcctggag gaatacacta tcaagacaat agttaaaaag tattcggatt
     1681 atgttcgcta tcctatcaga atgacggtag agaagagacg tcttaaggag ggtaccgagg
     1741 acgaatacga aactgtaaaa gaggacgaga cattaaacag tatgattcct ctttggaaaa
     1801 agaataaaaa agagattaag cccgaggaat atgaagcttt ttataaagat aagtttatgg
     1861 actatgaaaa gccgctcaag gttattcatt ataaaaccga gggtcaggca acatatgacg
     1921 cgcttctttt cattccgtca cacgctccgt ttgattatta tacaaaggaa tacgaaaagg
     1981 gcttgcagct ttactcaaaa ggcgttttga taatggacaa gtgcaaggac ctcttgcccg
     2041 accatttcag ctttgttaag ggtcttgtag acagcgagga tctctcgctt aacatttcgc
     2101 gtgaggtgct tcagcatgac
//
LOCUS       DBMDVO010000189         2111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25180_length_2111_cov_1.144279, whole genome shotgun sequence.
ACCESSION   DBMDVO010000189 DBMDVO010000000
VERSION     DBMDVO010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25180_length_2111_cov_1.144279"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="ACZPE2_03545"
     CDS             <1..1786
                     /locus_tag="ACZPE2_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR1318956.1"
                     /translation="TLDGVDKYVTYAPIANTEGWSCLVVATPSEYTDSLKMSLYIGTA
                     VAVICFVVSVLVILTIVKKVIKPVKLCSDRIVTLSQGDLHAEPLDFGKNIDNEISQLS
                     ESTNQITTQINAIITDLDNMLAALGNGDLTYSPADVYIGDFAKLRASYERIMLSLNKT
                     MSGISTAGVQVSNGAEQVSSAAANLSEGATRQAASVEELSASLAEVSEKVNRNAARAG
                     DAAKNSEQAAKLVDSGNEKMNILLEAMHKIDDTSKQIANIIKAIDDISFQTNILALNA
                     AVEAARAGEAGKGFAVVADEVRNLAGKVAQAASDTTTLIGDSMKAVEDGTAIANDTAQ
                     TLALIVETTTQTADLIEDISTACEEQATAISQITAGVDQISSVVQTNSATSEECAASA
                     EELSSQATILDGMVNKFKLSQKAIAEEPVKAAGKASESEKTASAKPEKADRPEAKKPS
                     EPKRDKEKTVKSKQEIKKTTAEAKEIKAEKPAVASKPADIITDKKAPESKPLASMPIK
                     KNESKPVASTQIKKNESKPVANTQIKKNESTAENKKKNSESAIKAISADFKTEAPAQK
                     KTNAIGISHFDTAVNSENEFTEDANDKY"
BASE COUNT          681 a          478 c          502 g          450 t
ORIGIN      
        1 tacgcttgac ggcgtggata aatatgtaac ctatgctcct attgcgaata cggaaggatg
       61 gagctgtctt gttgtcgcta ccccgtctga atacacagac agcttaaaga tgtcgctgta
      121 tataggaact gcggtagccg ttatctgctt tgtcgtatcg gttctggtaa tcctcacgat
      181 agtcaagaag gtaatcaagc ccgtaaagct ctgctctgac aggatagtga cgctttcaca
      241 gggagatctt catgccgagc cgctcgactt cggcaaaaat atcgacaatg aaataagtca
      301 gctcagcgaa agcacgaatc agataacaac acagataaac gcaattatca cagaccttga
      361 caatatgctc gctgcactcg gaaacggcga tcttacatat tctcccgccg atgtttatat
      421 tggtgatttt gcaaagctga gagcttcata cgaaagaata atgctctcgc tcaacaagac
      481 aatgagcggt atcagcacag caggcgttca ggtatcgaac ggtgcggagc aggtatcatc
      541 tgccgccgca aatctttcgg agggtgctac aaggcaggcg gcttctgtgg aagagctttc
      601 cgcttcgctt gcggaagtat ccgaaaaggt aaaccgcaat gccgcaagag ccggcgacgc
      661 agccaagaat tccgaacaag ccgcaaagct ggttgattcg ggaaatgaga agatgaatat
      721 tctgcttgag gctatgcaca agatagacga tacttcaaag cagatagcaa atatcataaa
      781 agccatcgac gatatttcgt tccagacgaa tattcttgca ctcaatgccg ccgttgaagc
      841 ggcaagagcg ggagaagccg gcaagggttt tgccgtagtt gcagacgagg taagaaatct
      901 tgcaggcaag gttgcacagg cggcaagcga taccacaacg cttatcggag attctatgaa
      961 ggcggtagag gacggtacag caatcgcaaa cgatacggcg caaacgcttg ctcttatcgt
     1021 tgaaactacc acgcagacgg ctgatctgat tgaggatatt tcaacagcct gcgaagaaca
     1081 ggcaacagct atatctcaga taacagcagg tgtcgatcag atttcctccg ttgtacagac
     1141 aaactccgct acaagcgaag aatgcgccgc atcggcagaa gaattatctt cacaggcgac
     1201 tatccttgac ggtatggtaa acaaattcaa attaagccaa aaggcaattg cggaagaacc
     1261 tgtgaaagcc gcaggcaagg cttccgaaag cgagaaaaca gcttcggcga aacctgaaaa
     1321 agcagatagg ccggaagcta aaaagccgtc cgagccaaag cgtgacaagg aaaaaacggt
     1381 aaaaagcaag caggaaataa agaaaaccac tgcggaggct aaagaaataa aagccgagaa
     1441 gcctgctgtc gcaagcaagc ctgccgatat aatcacagat aaaaaggcac ctgaaagcaa
     1501 gccgcttgca agtatgccga taaagaaaaa cgaaagcaag ccggttgcaa gtacgcagat
     1561 aaagaaaaac gaaagcaagc ctgttgcaaa tacgcagata aagaaaaacg aaagcacagc
     1621 cgaaaacaag aagaaaaaca gtgaatcggc aataaaagcc atatctgccg acttcaaaac
     1681 ggaggctcct gcacaaaaga aaacgaatgc tataggaatc tcgcattttg acacggctgt
     1741 aaattcggaa aatgagttta cagaggacgc aaacgataaa tattaagaat tgaggtgccg
     1801 tttttgcggc acctcggttg gtataaaaag tcttgtattt gatgtacttc tgttataact
     1861 tttagtattt gtctatgata gcaagctacc gatagttctt tttgtcagac agcacttctc
     1921 ggtttcgcta ttcgaattaa tacacctgtc aatattaacg agggaaagaa ccaaagagac
     1981 tagggtaagg gacttgacag cccctctccc tatctcttag gttctctccc tcgtggctcc
     2041 ctctttcctt actcacaccc ccaccccgtc ggggtgttgg ctaaactcga ataaccacct
     2101 aaacattgta t
//
LOCUS       DBMDVO010000190         2096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25416_length_2096_cov_1.256642, whole genome shotgun sequence.
ACCESSION   DBMDVO010000190 DBMDVO010000000
VERSION     DBMDVO010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25416_length_2096_cov_1.256642"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1063)
                     /locus_tag="ACZPE2_03550"
     CDS             complement(<1..>1063)
                     /locus_tag="ACZPE2_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR1318957.1"
                     /translation="HNIEIVVDRLVINDDIRSRLAEAIEMSSTLTGGNVIVDVIGGDE
                     LLFSQNFACPEHGACIDELSPRMFSFNNPFGACPTCTGLGIFLKVDPNLIIPNKKLSI
                     REGAIRASGWSNADSGTIAEMYYLALGKEYGFTLDTPICDFSNEAYNALLYGTGGKKL
                     KMQRKNVYGTGTYNTEFEGIVNNMERRYKESTSDWARWEIEQVMTACDCPDCKGARLK
                     KESLSVTVGGLNIYELTKLSVTKELDFINTLVLTDHEQMIASLILKEIKSRLSFLQNV
                     GLDYLTLSRSAGTLSGGESQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNDKLIGTL
                     KRMRDLGNTLIVVEHDEDTM"
     assembly_gap    1064..1073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1074..1658)
                     /locus_tag="ACZPE2_03555"
     CDS             complement(<1074..1658)
                     /locus_tag="ACZPE2_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1318958.1"
                     /translation="MASNKIIIKGAKEHNLKNIDLEIPRDKLVVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARMFLGQMEKPNVDYIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEIYDYLRLLYARIGIPHCPICGREISKQSVDVIVDKVMALDSGTKIQILAPVVKGR
                     KGEHQKELDSARKSGFVRVRIDGNLYDLSEDIKLE"
     gene            complement(1658..>2096)
                     /locus_tag="ACZPE2_03560"
     CDS             complement(1658..>2096)
                     /locus_tag="ACZPE2_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR1318959.1"
                     /translation="GLDIPEVSLVAILDADKEGFLRSETSLIQTIGRAARNADGQVIM
                     YADSVTPSMEKAISETYRRREIQTAYNKEHHITPKTIKKDVRDIIEISTHADDKPKKR
                     LSAREREALIVKLTAEMKAAAKILEFEHAAMLRDKIQKLREGK"
BASE COUNT          539 a          508 c          415 g          624 t
ORIGIN      
        1 catggtgtct tcatcgtgtt caacaactat gagcgtatta ccgaggtcac gcatacgctt
       61 aagcgttccg ataagcttat cgttatcgcg ctgatgaagt cctatgctcg gctcgtcaag
      121 aatatacaac actcccataa gagacgagcc tatttgagta gcaagtctta tgcgctgact
      181 ctctcctccg gaaagcgtac ctgcagaacg agacagcgta agataatcaa gaccgacatt
      241 ttgaaggaag ctcagacggc ttttaatttc tttcaaaatc aacgacgcaa tcatctgctc
      301 atggtcggtc aaaaccaaag tgtttataaa atcaagttcc tttgtcaccg acagctttgt
      361 aagctcatag atattaagtc cgcccaccgt aacggaaaga gactcttttt ttaatctcgc
      421 gcccttacaa tcggggcaat cgcaggctgt cataacctgc tctatctccc aacgcgccca
      481 atcgcttgta ctctctttat agcggcgttc catattgttt acgatgccct caaactcggt
      541 attgtaggta cctgtgccgt aaacgttttt acgctgcatt ttcagctttt tgccgccggt
      601 tccgtagaga agcgcattat acgcttcatt ggagaagtcg caaatgggtg tgtcgagagt
      661 aaaaccgtat tccttaccga gtgccagata gtacatttcg gctattgtgc cgctgtcggc
      721 atttgaccat ccgcttgccc ttatagcgcc ttctcttatg gaaagctttt tattcggaat
      781 tataagatta gggtctacct taagaaaaat gccgagcccg gtacaagtcg gacacgcacc
      841 gaaaggatta ttgaacgaga acatacgcgg cgacaactca tcaatacaag caccatgttc
      901 ggggcacgcg aaattctgtg aaaacagcaa ttcatctccg ccgataacat caacaatgac
      961 atttccgccc gtaagagttg atgacatctc tatcgcctcg gcaagacgac tacgtatatc
     1021 atcgtttata acaagcctgt caactactat ttcaatgtta tgcnnnnnnn nnnttcaagc
     1081 ttaatatcct cggaaaggtc atataaatta ccgtctatac gcacacgtac aaatcccgac
     1141 tttcttgcgg aatcaagctc tttttgatgt tcgccctttc gtccttttac cacaggtgcc
     1201 aaaatctgta tttttgtgcc gctatcaagc gccataacct tatcaactat aacgtctaca
     1261 ctctgctttg atatttccct accgcaaatc ggacaatgcg gaatacctat acgggcatat
     1321 aaaagcctta ggtaatcata aatctccgta accgtgccga ctgttgagcg tgggtttttt
     1381 gaagtggttt tttggtctat ggatatagcc ggagaaagac cgtctatata atcgacattc
     1441 ggtttttcca tctgccccaa aaacatacgc gcatatgaag aaagtgattc aacgtatctg
     1501 cgctgaccct cggcataaat tgtgtcaaac gcaagcgatg actttccgga tcccgaaagc
     1561 cccgtaaata ccacaagctt gtctcgcggt atttcaaggt caatattttt taagttgtgc
     1621 tcttttgcac ctttgataat gattttatta cttgccatta tttaccttct cttaattttt
     1681 gtatcttatc gcgcagcata gcggcgtgtt caaattcaag aatttttgca gccgctttca
     1741 tctctgccgt caatttaact ataagagcct cacgttcacg cgcgctgaga cgctttttgg
     1801 gtttatcgtc agcgtgagtt gatatttcaa taatatcgcg cacgtctttt ttaatcgttt
     1861 ttggcgttat atgatgttcc ttattataag ccgtttgtat ctcacgcctg cgatacgttt
     1921 cggatattgc tttttccata gacggagtta cggaatcggc atacattatt acctgcccgt
     1981 cggcatttcg cgcggcacgg cctatggttt gtataagaga cgtttccgaa cgtaaaaatc
     2041 cctctttgtc ggcgtctagt attgccacaa gcgaaacctc gggaatatca agtccc
//
LOCUS       DBMDVO010000191         2096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25419_length_2096_cov_1.009023, whole genome shotgun sequence.
ACCESSION   DBMDVO010000191 DBMDVO010000000
VERSION     DBMDVO010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25419_length_2096_cov_1.009023"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="ACZPE2_03565"
     CDS             complement(<1..512)
                     /locus_tag="ACZPE2_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1318960.1"
                     /translation="MSSPLADRLRPEKIEDMVGQKHLLAEGMPLRNILDSGTLPNLIF
                     YGPSGTGKTTLAKIIAKTTNRKLHKLNGTNASTADIKEIVSELDTLAAPNGILLYLDE
                     IQYFNKKQQQSLLQYIENGAITLIASTTENPYFYIYSAVLSRSTVFEFKSVTAEDMLP
                     AIDRGYKFLE"
     gene            complement(612..1268)
                     /locus_tag="ACZPE2_03570"
     CDS             complement(612..1268)
                     /locus_tag="ACZPE2_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1318961.1"
                     /translation="MKLNFGTVLFDFDGTLFDSSEGIFKSLKYAFRADNKPEPTDGEL
                     RRFIGPPIYDSFKSFYSYDDEKIDFMVEKYRERYREKGLWESRAYDGIPELLKKLRDC
                     GVKTATASSKPEQFIISILKKNNLYDYFDYVGGVTFDEIRSDKTQIIENALKKLGSSK
                     NDAVMVGDRKFDIDGAKGAGIPCIAVLYGFGSLEEFREHKADYIVSTPAEIEKLIISG
                     "
     gene            complement(1280..>1709)
                     /locus_tag="ACZPE2_03575"
     CDS             complement(1280..>1709)
                     /locus_tag="ACZPE2_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318962.1"
                     /translation="TDNGFNTMKYLNGQSSVINMFYGTYGSSYVRSGDWILSKEDGRL
                     FNTEDNTVIETVGYKMNPLLFTVSPDGKYVVMLGTVTNAMDYRMYVFNTETKGYKVYT
                     ESNYAAHYNLRFSSNDTVTFYTIDANGAYINVVADVSKVK"
     assembly_gap    1710..1719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1720..2065)
                     /locus_tag="ACZPE2_03580"
     CDS             complement(<1720..2065)
                     /locus_tag="ACZPE2_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318963.1"
                     /translation="MLLLHTDAQKVYTDNVVWEEAYILNRNDGTSTRFLNENNRTVNI
                     NGAVRPDFCMITDTELKSTTSVIPFFSCRNYEEQEDGSTPKDIYIKNGNNKDVDAVTD
                     VLDTYAKPTSDGG"
BASE COUNT          524 a          488 c          444 g          630 t
ORIGIN      
        1 ttctcaagaa atttataccc gcggtcaatc gccggaagca tatcctcggc tgtaaccgat
       61 ttaaattcaa ataccgttga acggctgaga accgcgctgt atatataaaa ataaggattt
      121 tcggttgttg aagctataag agttatagcc ccgttttcta tgtactgcaa aagcgattgc
      181 tgctgttttt tattgaaata ctgtatctcg tcaaggtaaa gcaatattcc gttaggagcc
      241 gcgagggtgt caagctccga aactatttct ttaatatccg cagttgacgc atttgttccg
      301 ttgagcttat gcagcttgcg gtttgtggtt tttgctataa ttttggcaag cgtcgttttg
      361 cctgtgcccg acggcccgta aaatatcaga ttcggaagcg tacccgaatc caagatattg
      421 cgaagcggca taccctctgc caaaaggtgc ttttggccga ccatatcttc gattttttcg
      481 gggcgtagcc tgtcagctaa cggtgaagac atatttttac cgcctttccg actgcatatc
      541 cataataaag aataaagcgg cgcgccccgt tgcagtcgta aaggacgcgc gtcgactaaa
      601 attttttgtt tttatccgga aataatcagc ttttcgattt cagcgggagt cgaaacaata
      661 taatccgctt tgtgctcacg aaattcctca agactgccga agccgtaaag caccgctatg
      721 cacggtatgc ctgctccctt tgcaccgtcg atgtcaaatt ttctgtcgcc gaccataacg
      781 gcgtcgtttt tggatgagcc gagcttcttc agcgcatttt cgattatctg cgtcttatcg
      841 gagcgtattt catcaaaagt gacgcctcct acatagtcga aataatcgta taggttgttt
      901 ttcttcagaa tgcttattat aaattgctcc ggctttgacg acgcggtggc tgttttcacg
      961 ccgcagtctc gcagtttttt caaaagctca ggtataccgt cgtaagccct tgattcccaa
     1021 aggccctttt cgcggtatct ctcgcggtac ttttccacca taaaatctat tttttcatcg
     1081 tcgtaggaat aaaagctctt aaagctgtca tatataggcg gtcctataaa acggcgaagc
     1141 tccccgtccg tcggctccgg tttattatcc gcccgaaaag catatttaag acttttgaat
     1201 attccctccg acgagtcaaa aagagttccg tcgaagtcaa aaagcactgt gccgaagtta
     1261 agtttcataa aatcctcgct tatttcacct ttgatacatc ggcaacgaca tttatataag
     1321 caccgtttgc gtcaatcgta tagaaggtaa ctgtatcatt tgaagaaaat ctgagattgt
     1381 aatgagcggc ataattactc tcggtataca ccttatagcc ctttgtctcg gtattaaata
     1441 catacatacg gtaatccatg gcatttgtca ccgtaccgag cattacaacg tattttccgt
     1501 cgggacttac ggtgaaaaga agcggattca ttttatagcc taccgtctct ataaccgtgt
     1561 tatcctcagt attaaacagt ctgccgtcct ctttcgaaag aatccaatcg ccgcttctga
     1621 cataagagct gccgtatgtt ccgtagaaca tatttattac actcgactga ccgttaagat
     1681 acttcatagt attaaaaccg ttgtcggttn nnnnnnnnna gccgccgtcc gacgtgggct
     1741 tcgcatatgt atcgagcacg tctgttaccg cgtcaacatc tttattgttg ccgtttttga
     1801 tgtaaatatc cttcggagtg ctgccgtcct cctgctcctc gtagtttctg cacgagaaga
     1861 acggtattac ggaggtagta gatttaagct ccgtatcggt tatcatacag aagtccggac
     1921 gaaccgctcc gtttatgttt acggtacggt tgttctcatt gagaaatctc gttgaggtgc
     1981 cgtcgttacg attgagaata tacgcttcct cccagacaac attgtcggta tacacctttt
     2041 gagcgtctgt atgaagaagg agcaagcact taccgtccgc agcgtaagcc gatgga
//
LOCUS       DBMDVO010000192         2095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25434_length_2095_cov_1.332999, whole genome shotgun sequence.
ACCESSION   DBMDVO010000192 DBMDVO010000000
VERSION     DBMDVO010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_2095_cov_1.332999"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="ACZPE2_03585"
     CDS             complement(<1..663)
                     /locus_tag="ACZPE2_03585"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR1318964.1"
                     /translation="MNIAIVGLGLIGGSIAKAIKYNTDNTVYGYDIDRGVLLKAKLLG
                     AVDEELTSDILPTCDMVILGLYPNDTLEFVRTHADKIKKGAIVMDSCGVKRVVCEPLW
                     KVASENGFTFIGAHPMAGLHFSGFEYSDVGMFSEASMIIIPSKDVKIEDLEAVKSLYL
                     KLGFTNIQISTPEEHDKIIAYTSQLAHVVSNAYVKSPEAEVHKGFSAGSYKDLTRVAK
                     LNE"
     gene            complement(682..1689)
                     /gene="aroF"
                     /locus_tag="ACZPE2_03590"
     CDS             complement(682..1689)
                     /gene="aroF"
                     /locus_tag="ACZPE2_03590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1318965.1"
                     /translation="MVVILKDGADSKKVKNLTDWLENQKISVHKVIGEYQTILGLIGD
                     TSKIDIDLIQGLDIVEKVKRISEPYKNANRKFHPDDTIIDVGGHKIGGGNFQYFAGPC
                     SVESREQIISIAKDVKKAGANILRGGAFKPRTSPYAFQGMRAEGLELLLEAKKETGMP
                     IVTEIMDASHIDLFENVDIIQVGARNMQNFELLKELGRIDKPIFLKRGLCATLQEFLM
                     SAEYIMAEGNQKVILCERGIRTFETATRNTLDLSAVAMLHEMTHLPVVVDPSHASGIA
                     RLVKPMSFAAAACGADGLMIEVHNDPAKALCDGPQALRPEEFAEVTRGVNAIRETVNN
                     L"
     gene            complement(1942..>2095)
                     /locus_tag="ACZPE2_03595"
     CDS             complement(1942..>2095)
                     /locus_tag="ACZPE2_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HIR1318966.1"
                     /translation="VVTHEMGFAREVGDRVLFMDDGKILEENAPEELFTNPQNPRLQE
                     FLSKVL"
BASE COUNT          538 a          509 c          440 g          608 t
ORIGIN      
        1 ctcattgagc tttgcgacac gcgttaaatc cttgtagctg cctgccgaaa aacccttgtg
       61 cacttccgcc tcggggcttt taacataggc gtttgaaacg acgtgggcaa gctgagaggt
      121 ataggctatt atcttgtcgt gctcctcggg agtcgatatt tgaatatttg taaatccgag
      181 ctttagatat aagcttttaa cggcctccaa gtcctcgatt ttgacatcct ttgatgggat
      241 aattatcatg gaagcctcgg agaacatacc gacatcggaa tattcaaagc ccgagaagtg
      301 aagtcctgcc attgggtgcg ctccgataaa cgtaaagccg ttttcggagg ctaccttcca
      361 gagcggctcg cagactacgc gctttacgcc gcaggaatcc ataacaatgg cacctttttt
      421 tatcttatcc gcatgagtgc gtacaaattc gagcgtatca ttggggtaaa ggcccaaaat
      481 caccatatcg cacgtcggga gaatgtcact tgtgagttcc tcgtcaacgg ctcccaaaag
      541 ctttgctttc agcagaactc cgcggtcgat gtcatagccg taaacggtat tgtccgtatt
      601 gtatttgatt gccttggcta ttgagccgcc gataagtccc aaacctacta ttgctatatt
      661 catctttatc gcctctcaaa attacagatt gtttaccgtt tctcttatag cgttaacgcc
      721 tctcgttacc tctgcgaatt cctccggacg aagagcctga ggaccgtcgc aaagagcctt
      781 tgcagggtcg ttgtgaacct ctatcataag gccgtctgcg ccgcaggcgg cagccgcaaa
      841 ggacatcggc tttacaagtc ttgcgatacc cgaagcatgg ctcgggtcaa ctactacggg
      901 gaggtgcgtc atttcgtgga gcattgcgac agccgaaaga tcgagggtat ttctcgtggc
      961 ggtctcaaag gttcttatgc cgcgctcgca gagtataacc ttttggttgc cctcggccat
     1021 aatgtattcg gcactcataa ggaattcctg aagcgtagcg caaagtccgc gctttaagaa
     1081 aatgggtttg tctattctgc cgagctcctt taaaagctca aaattctgca tattccttgc
     1141 tccgacctga attatgtcca cattctcaaa caaatcaata tgagacgcgt ccataatttc
     1201 ggtaactatc ggcatacctg tttctttttt cgcctcaagc aaaagctcaa gtccctcggc
     1261 gcgcattccc tggaaagcat agggcgaagt gcgcggtttg aaggcgccgc cccttaaaat
     1321 attggcaccg gcctttttga cgtcttttgc tatcgagatt atctgctcgc ggctctcaac
     1381 cgagcaagga cctgcaaaat actggaaatt tccgccgcct attttatgtc cgccgacatc
     1441 aataatcgtg tcgtccggat gaaattttct gttggcgttt ttatacggct ctgaaattct
     1501 ttttaccttt tcaactatgt caaggccctg aataaggtca atatcgattt tgctcgtatc
     1561 accgataagg cctaaaatcg tctggtactc gcctataacc ttatgaaccg aaattttctg
     1621 attttcgagc cagtcggtaa gattttttac ttttttactg tctgcaccgt cttttaaaat
     1681 tactaccatt gttttgctcc ttactctgaa aaataaaaag tcccgcagcg aatttcactg
     1741 cgggacgcat ttaccaaaat accttaaatc tcagcacgct atctgttttg cagcaaaatt
     1801 catactaacc ctaccaaaaa agttgataaa gtaaaagtaa tagtatgaag ctgtaaagac
     1861 aagattacgc attgattatt acctcaatat tttagcggtt taaagtataa taataccaaa
     1921 gatttgattt gtcaacactt tttataaaac ttttgaaaga aattcctgca gcctcggatt
     1981 ttgcggattc gtgaaaagct cctcaggagc attttcttcg agaatctttc cgtcgtccat
     2041 aaacaaaact ctgtcgccga cctcgcgtgc aaagcccatt tcgtgggtaa caaca
//
LOCUS       DBMDVO010000193         2095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25435_length_2095_cov_1.255767, whole genome shotgun sequence.
ACCESSION   DBMDVO010000193 DBMDVO010000000
VERSION     DBMDVO010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25435_length_2095_cov_1.255767"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(189..280)
                     /locus_tag="ACZPE2_03600"
     tRNA            complement(189..280)
                     /locus_tag="ACZPE2_03600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Ser,seq:gga)
     gene            628..1881
                     /locus_tag="ACZPE2_03605"
     CDS             628..1881
                     /locus_tag="ACZPE2_03605"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR1318967.1"
                     /translation="MSYNPYDNVLATVENAANILGYKPAEYEAVKYPERELSVSVPVR
                     MDDGSVKVFKGYRVQHSTVRGPAKGGIRYHQNVNLDEVKALSAWMTFKCAVADIPYGG
                     GKGGVVVDPTTLSDGEKQRLTRRFTSMISPIIGPDKDIPAPDVGTNAEVMGWIMDTYS
                     MLNGHSTPAVVTGKPIALGGSEGRNEATGRGIMLNTLYICQKLGIDIKAATVTIQGMG
                     NVGSITAKLLNKEGAKIVAVSDVSGGIYNENGLNIPAILEYLSVKGNLLSGYNEDGMK
                     RISNEELLTLDTTILIPAALENQINKDNADKIKAKVIVEGANGPTTIEADEILDKKGV
                     VLVPDILANSGGVIVSYFEWVQNLQSFEWTEEEVNSKLARKMSKAFENVYGIAKEKNV
                     SLRTGAYLIALKSVVDTTKLRGIWP"
BASE COUNT          652 a          439 c          450 g          554 t
ORIGIN      
        1 gcatagggac aggtcttgcc tctaagggac tcggctcact tcgttcgccc tcgagtttgc
       61 tccgcaaact atcctattat agacttcttt tgacaagact tcgtcttgca aaagattata
      121 agggttcgaa tcccttttta atttaaagca aaaaacagga catagctttt gctatgccct
      181 gttttttatg gcggagagca agggattcga accctcgaac cgttttaggc ggttacacga
      241 tttccaatcg tgctccttcg accagctcgg acaactctcc atagatcaaa tgacgaggaa
      301 tattatatat gattttattc aaaaaagcaa gcaaattttt tcgtattatt caaaaattgc
      361 tctatcggcg catatccata taaaacggtc tccgatgtgc ctgccgagtg aacctaagac
      421 accgatattc cttcaatcgt aaaaacacaa caaacacgaa gcaacaacgt atagaagtta
      481 tgaacaattc gaaagtttct gttttgtgca tattaaatat aattttaatt tatataaaaa
      541 atattattga caaaatgcaa aaaagctgta atatagtatt aaagtgaatt tatattcacc
      601 aataaaagat gaatggagtg ttaagtaatg agttataatc cttatgacaa cgtgctcgca
      661 acagttgaaa acgcagcgaa tattctcggc tacaagccgg cggaatacga agctgtgaag
      721 taccccgaaa gagaactctc cgtttctgtt ccggtaagaa tggacgacgg cagcgtaaag
      781 gtatttaaag gttatcgcgt tcagcattct acggtacgcg gccccgcaaa gggcggaatc
      841 cgttatcatc aaaacgtcaa tcttgacgaa gttaaggctt tgtcggcttg gatgacattc
      901 aaatgcgctg ttgccgatat tccctacggc ggaggcaagg gcggcgttgt tgttgacccg
      961 acaactcttt ccgacggcga aaagcaaagg ctcacaaggc gcttcacctc tatgatatcc
     1021 cctataatcg gacccgataa ggatataccc gctccggacg tcggaacaaa cgccgaggta
     1081 atgggctgga taatggatac atacagtatg ctgaacggtc actccactcc ggcggtagtt
     1141 acgggcaagc cgatagctct cggcggaagc gaaggaagaa acgaggccac cggccgcggt
     1201 ataatgctta acactcttta tatatgccaa aagctcggca tcgatattaa agcggccacg
     1261 gtaacaattc aaggtatggg taacgtcggc agcataacgg caaagctcct caataaagag
     1321 ggggcaaaaa tagtagctgt cagcgatgtt tccggcggta tctacaatga aaacggtctt
     1381 aatatacctg caattcttga atatctctcc gtaaaaggca accttctttc gggctacaat
     1441 gaggacggta tgaagagaat ttcaaacgaa gagcttttaa cgcttgacac aaccattctc
     1501 attcccgcag ctcttgagaa ccaaataaat aaagataatg cagataaaat caaagctaag
     1561 gttatagtcg agggcgccaa cggccctaca acaattgaag cggacgaaat actcgacaaa
     1621 aaaggtgttg tattggttcc cgatattctt gcaaactcag gcggagttat agtttcgtat
     1681 tttgagtggg tgcaaaatct tcagtcgttt gaatggaccg aagaagaggt aaactctaag
     1741 cttgcaagaa aaatgagcaa agcatttgaa aacgtatacg gcattgccaa agaaaaaaat
     1801 gtttcgctca gaaccggagc ttatctcata gctcttaaga gtgttgttga caccactaag
     1861 ctaagaggca tttggccgta atttgaccga tatgacaaag aacgaaccac ctaaaggcgg
     1921 ttcgttcttt ttgtttaaaa taaaattgtg atttgtttga taatttatcc gattatcttt
     1981 tatcagcttt cgtttttatc ttttgacggc ttttcttttt tgcgagtctt attaaacagc
     2041 gcggcagccg taaccgcaag tatgacagct ataattcccg acgtaacacc gataa
//
LOCUS       DBMDVO010000194         2092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25490_length_2092_cov_0.958815, whole genome shotgun sequence.
ACCESSION   DBMDVO010000194 DBMDVO010000000
VERSION     DBMDVO010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25490_length_2092_cov_0.958815"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2092)
                     /locus_tag="ACZPE2_03610"
     CDS             complement(<1..>2092)
                     /locus_tag="ACZPE2_03610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1318968.1"
                     /translation="LHRLGIQAFEPVLVEGRAIKLHPLACTAFNADFDGDQMAVHVPL
                     SAEAQAEARLLMLAANNLLKPSDGKPVTVPTQDMILGSYYLTMVKDGEPGEGKAFLSV
                     DEATMAYNEGDIGLHSKIKIRMTKEINGEKVTKLVPTTLGKIIFNNPIPQDLGFIDRS
                     NPENAFNLEVEFLVDKKGLGKIIDKCIKKHGVHIASVMLDSIKAQGYKYSTKSGITVA
                     VCDAIIPPHKQELLEAAEEKVDSITAQYKMGLLSNNERSKRVIKVWEECTNKVSQGIK
                     ENLGKNNPIYMMVDSGARGSESQLRQLAGMRGLIANTAGKTIEVPIRANYREGLNVLE
                     YFISSRGARKGLADTALRTADSGYLTRRLVDVSQDVIIREEDCHCHDGIEVYDIHEGK
                     EMIETLQERLTGRFLLEDFVDEKTGELIMSKDKMMTEDDAAKVAAIVNARPNEADRRI
                     KIRSLLTCESDHGVCIKCYGSNLATGEPVTVGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GIASSQDITQGLPRVEELFEARKPKTLAILAEIDGYLSFREIKKNEHIVITNPETAEE
                     KTYLIPYGFRKKFNSDKDENGNPIFIKKGTALTEGAPNPHDILAINGVNAVHDYLIKE
                     VQKTYRMQGVDINDKHIEVIVRQMMRKLKVDEQGSTELLPGATIEKSDFNKENKRVSA
                     ECEAEGKEFTPATC"
BASE COUNT          480 a          513 c          510 g          589 t
ORIGIN      
        1 tcgcaggtgg ccggagtaaa ctccttaccc tctgcctcgc actctgcgct gacgcgcttg
       61 ttctctttgt tgaagtcgga tttttcaatg gtagctccgg gaagaagctc ggttgaaccc
      121 tgctcatcca ccttgagctt tctcatcatc tgacgaacga taacctcaat gtgcttatca
      181 ttgatatcaa caccctgcat acgataggtc ttctgaactt ccttgataag gtagtcgtga
      241 accgcattga cgccgttgat ggcgagtatg tcgtggggat tgggagcacc ctctgtaaga
      301 gcggtaccct tctttatgaa tatcgggttg ccgttttcat ccttatcgct gttgaacttc
      361 ttgcggaagc cgtaaggaat aaggtaggtt ttttcttcgg ccgtctcggg attggtgatg
      421 acaatgtgct cattcttttt gatttcgcgg aatgagagat agccgtcaat ctcggcaaga
      481 attgcgagag tcttgggctt tcttgcctca aacaattcct cgacacgggg aagaccctgt
      541 gtaatatcct ggcttgatgc gataccgccg gtatggaagg tacgcattgt aagctgtgta
      601 ccgggctcac ctattgactg agccgctatg ataccgacag cctcgcccac ggtaacaggc
      661 tcgcctgtcg caagattcga accgtagcac ttaatgcaaa cgccgtggtc cgattcgcag
      721 gtgagaagcg aacgtatctt tattcttctg tctgcttcat tgggacgggc gttaacgata
      781 gccgcgactt tagctgcatc gtcctcggtc atcatcttat ccttggacat aataagctcg
      841 ccggtttttt cgtctacaaa atcctcaaga aggaatctgc ctgtaagtct ctcctggaga
      901 gtctcaatca tttccttgcc ctcgtggata tcgtaaacct caataccgtc atggcaatgg
      961 caatcctctt cacggattat aacgtcctgt gaaacatcga caagacgacg ggtaaggtaa
     1021 cccgagtcag ccgttctaag tgcggtatcg gcaagacctt tacgcgcacc acgcgatgag
     1081 atgaagtatt cgagtacatt aagaccttca cggtaattgg ctcgaatcgg aacctcaatg
     1141 gttttacctg ccgtatttgc aataagtccg cgcataccgg caagctgacg aagctgtgat
     1201 tccgaaccac gggcaccgga gtcaaccatc atataaatgg gattgttttt accgaggttc
     1261 tctttaatac cctgagaaac cttattggtg cattcttccc aaaccttgat gactctcttt
     1321 gaacgctcat tgttgctcaa aagacccatc ttatactgtg cggtgatgga gtcaaccttt
     1381 tcctcggctg cttcaagaag ctcttgcttg tgcggcggga ttatagcgtc gcatacggca
     1441 accgttatac cggattttgt agagtattta tagccctgag ccttaatgct gtcaagcatt
     1501 acggaggcta tgtgtacgcc gtgcttttta atgcacttat cgattatctt gccgagaccc
     1561 ttcttatcga caaggaattc aacctcaaga ttgaatgcgt tttcgggatt gcttctgtcg
     1621 ataaagccga gatcctgcgg aatgggattg ttgaatatga ttttaccgag agttgtggga
     1681 acaagcttag taaccttttc accgtttatc tctttggtca tacgaatttt aatttttgaa
     1741 tgaagaccga tgtcgccctc gttgtaagcc atggtagcct catcaacgct gaggaacgct
     1801 ttgccttcgc ccggctcgcc gtcttttacc atagtgaggt agtaagaacc gagtatcata
     1861 tcctgtgtgg gaacggtaac cggtttaccg tcggacggct tgagaaggtt atttgcggca
     1921 agcataagga gccttgcctc tgcctgagcc tctgccgaaa gcggtacgtg aacagccatc
     1981 tggtcgccgt cgaagtccgc gttgaatgcg gtacaagcaa gcgggtgaag cttaatggct
     2041 ctgccctcta cgagtaccgg ctcaaacgcc tgaataccga gtctgtgaag ag
//
LOCUS       DBMDVO010000195         2083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25618_length_2083_cov_1.597881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000195 DBMDVO010000000
VERSION     DBMDVO010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25618_length_2083_cov_1.597881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2083)
                     /locus_tag="ACZPE2_03615"
     CDS             complement(<1..>2083)
                     /locus_tag="ACZPE2_03615"
                     /inference="COORDINATES: protein motif:HMM:NF039835.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1318969.1"
                     /translation="FVGEYKNGEDLLGTVTAKVGETGLPAPGGTVTGDVTFTAVPQNG
                     YTLKSWRITDAENNTDIVNAAGSNTYTLTLGSTRYTVEAEFERVSETDRPVKLTYSVA
                     SGNETGMAATKQTYQSGTAVPVGTKINFIAQAAEGYRVQKLVITKNGIPDTVITDNRL
                     YDMYEYELTVDTQTDIKVYFTEIEKYTVTVDMPNKNAEITVKNGTSDFESGQKVNFGD
                     EITVTVKPKDGYRLADTESWSDNGDGSYTYKTGRIKMETHIRVAVEEIPEYTVTFPTM
                     IEGCVLTVRSKNEKITSGSKIREGTILVASIVLEPSYILKGWYIGGEFSPETNKTEVS
                     FTVDGDTLLSVIVENVKGDKGDTGIGIKSAIIDESGNLIITLTDDTVLNLGKVTGDNG
                     SQGEQGVPGAAGVGVKSVAIDENGNLVITLTDNSVHNLGKVIGDKGAPGETGAPGAAG
                     VGVKSAVIDKNGNLVITLTDNTVHNLGKVVGDKGEQGVNGVGVKSVMIDESGNLIITL
                     TDETVHNAGRVTGDKGDKGDTGNVGVGVKTTEIDENGNLIITLTDDTVHNLGKVVGAK
                     GEAGTAGTNGINGTGIKSAHIDADGNLILTFTDGVITNLGKIVGTDGKDGVNGKDGTN
                     GKDGKDGKDGLGIKGCRIDDGGDLIITLTDNTTLNAGNISAISDKVSVSKPLATAAVS
                     LSGVSLLWNIVS"
BASE COUNT          421 a          539 c          482 g          641 t
ORIGIN      
        1 gcgaaacaat attccaaaga agagacactc cggaaagaga aactgctgcg gttgcaagag
       61 gcttggaaac actgacttta tcgctgatcg cggaaatgtt gccggcgttc agcgttgtgt
      121 tgtcggtcag ggttattatc aggtctccac cgtcgtcaat gcggcatcct ttaataccga
      181 gaccgtcttt tccgtccttg ccatctttgc cattggtacc atctttacca ttgacaccat
      241 ctttcccgtc cgttccgacg atttttccga gattggttat aactccgtcg gtaaaggtga
      301 ggataagatt accgtctgcg tcgatatgag cggacttgat tccggtaccg ttaatgccgt
      361 tggttcctgc agtaccggct tcgccctttg caccgactac tttcccgagg ttatgcacag
      421 tgtcgtcggt aagggtgatg attagattgc cgttttcatc gatttcggtg gtttttacac
      481 cgactccgac attgcccgta tcgcccttgt cacctttatc tccggtcacc cgtccggcat
      541 tgtgtacggt ctcatcggta agagtgataa tcaaattgcc gctttcatca atcataacgg
      601 atttaacacc aacgccattc acgccctgtt cgcctttatc tccgactacc tttccgagat
      661 tatgcacggt attgtctgtg agagttatga ccagatttcc gtttttgtca atcacagcgg
      721 atttaactcc tactcctgcg gcaccgggag cacctgtttc gcctggggca cccttatctc
      781 cgatcacttt cccgaggtta tgcacgctgt tgtctgtaag tgttatgacc agatttccgt
      841 tttcatcaat tgcgacggat ttaactccta ctcctgcggc accgggaaca ccttgttcgc
      901 cctgacttcc gttatctccg gtgacctttc cgaggtttaa caccgtgtcg tcggtaagag
      961 taataatgag gtttccgctt tcatcaataa ttgctgattt gattccgatg ccggtatcac
     1021 ccttgtcgcc ttttacattt tcaacgatta cggaaagaag agtgtctccg tccaccgtaa
     1081 aggaaacttc ggtcttgttg gtttcgggac tgaattcgcc gccgatgtac cagcctttga
     1141 ggatatatga cggctcaagc acgatactcg caacaagaat tgtgccttca cgaattttac
     1201 tgccgcttgt gattttttca tttttgctgc gcactgtcag cacacagccc tcaatcattg
     1261 tcgggaaggt gacggtgtat tccggaattt cctctaccgc cacacggata tgcgtttcca
     1321 tcttgattct gccggtcttg taggtatagc tcccgtcgcc gttatcggac cagctctctg
     1381 tgtcggcaag acggtatcca tccttcggct tgacggttac ggtgatttca tcgccgaaat
     1441 tgaccttttg accgctttca aaatccgatg tcccgttttt aacggtaatt tcggcgttct
     1501 tattcggcat atcaactgtg accgtatatt tttcgatctc cgtaaagtat accttgatat
     1561 cggtttgggt atcaacggtg agctcatact cgtacatatc gtaaagcctg ttatctgtta
     1621 ttaccgtgtc gggaattccg tttttggtga taacgagctt ttgcacccta tagccttctg
     1681 ccgcctgagc gatgaagttg attttcgttc ccacgggaac agcggtaccg gactgatagg
     1741 tctgctttgt tgccgccata ccggtttcgt tgccgcttgc aacggaatag gtcagcttca
     1801 cgggtctgtc ggtttcggaa actctttcaa attccgcttc gacggtgtat cttgttgagc
     1861 caagggtaag cgtgtaggta tttgagcctg ccgcgttaac aatatccgta ttgttttcgg
     1921 catctgttat tctccacgac ttcagtgtat agccgttttg cggaacggcg gtaaatgtta
     1981 catcgccggt caccgtgccg cccggagcag gcagacctgt ttcaccgacc tttgcggtaa
     2041 ctgttccgag aaggtcttcg ccgttcttat attcgccgac aaa
//
LOCUS       DBMDVO010000196         2082 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25639_length_2082_cov_1.435134, whole genome shotgun sequence.
ACCESSION   DBMDVO010000196 DBMDVO010000000
VERSION     DBMDVO010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25639_length_2082_cov_1.435134"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>876
                     /locus_tag="ACZPE2_03620"
     CDS             <1..>876
                     /locus_tag="ACZPE2_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1318970.1"
                     /translation="LKEVIEVSPDFGKAVNVTRYGWLAVIFAAAAILVYIGGLMCSHL
                     SAFRIATNLRIDLTKHIASLPLGTTESFGTGKLRRIVSETAGAAETYLAHQLPDKYKA
                     MATILGLLALLFIFDWRLGLLSLVPVILGFLCMTKMTGKSMQKKMTEYQNALSDMSNE
                     AVEYVRGIPVVKTFGQTVFSFKKFKKAIDNYGKWTLAYTKELRGPMTAFTLAVNSVFA
                     FLILGAFLFTKDGVTGKFLLNLLFYIIITPAITTTLSKLMFMSENQMIVKDALARVDE
                     VLNAEPLSISEHPSH"
     assembly_gap    877..886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <887..1608
                     /locus_tag="ACZPE2_03625"
     CDS             <887..1608
                     /locus_tag="ACZPE2_03625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318971.1"
                     /translation="YDGKKDALSDITLKIGACETVAFVGPSGGGKSTLACLTARFFDP
                     QKGKVMIGGTDIRDIDKKELMNTVSFVFQNSRLIKASILENVKLGKPNASNEDVMKAL
                     KTAQCTDIIEKFPDGVDTVIGTNGVYLSGGEAQRIAIARAILKDSPIVILDEATAFAD
                     PDNEVKVQAAIRELSNGKTVIMIAHRLSTVANADKIFVLSDGKLAEEGKFDELRERGG
                     LFSSMWNEYQKSVCWKVSKEA"
     gene            1612..>2082
                     /locus_tag="ACZPE2_03630"
     CDS             1612..>2082
                     /locus_tag="ACZPE2_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1318972.1"
                     /translation="MIKKLQRRFALSRKGAVDTVKGSIFCALQNISFMLPVIMLYYLV
                     DELISNSLGRSRIPFYVIGCVICAAVIVICTILQYNGTFFTTYEESGVRRVSLAEQLR
                     KIPLSFFGKKDLADLTSSIMNDCAVLEQSQSHFVEPLIGSIISTVLIAVSLLFFN"
BASE COUNT          577 a          480 c          487 g          528 t
ORIGIN      
        1 tacttaaaga ggtaatcgaa gtatcgcctg attttggcaa ggcggtaaat gtgacgcgtt
       61 acggctggct tgcggtgatt tttgccgcgg cggcaattct cgtatacata ggcggactta
      121 tgtgctcgca tttgtcggca ttccgcatag ctacaaacct gcgaatagat ttaacaaaac
      181 acattgcctc gcttcctctc ggtacaacag agagcttcgg aacaggcaag ctgcgccgaa
      241 ttgtctcgga aaccgccggc gccgcggaaa catatctcgc acatcagctc cccgacaaat
      301 acaaagcgat ggcgactatt ttgggccttc tcgcgctgct gtttatattt gattggcggc
      361 tcggactgct aagccttgta ccggtgatcc tcggtttttt gtgtatgacg aagatgaccg
      421 gcaaaagtat gcaaaaaaag atgaccgaat atcagaacgc actctcggat atgtcaaacg
      481 aggcggttga atatgtccgc ggcatacctg tggttaaaac gttcggtcag acggtatttt
      541 cttttaagaa gtttaaaaag gcaatagata attacggcaa atggaccttg gcttacacaa
      601 aagagcttcg cggcccgatg accgcgttca cacttgccgt aaacagcgtg tttgcgtttc
      661 tcatactcgg agcgtttttg tttacaaaag acggcgtaac gggcaaattc ctgctgaatc
      721 tgctctttta cattataatc acaccggcaa taacaaccac tctctcaaag cttatgttta
      781 tgagcgaaaa tcaaatgata gtaaaggacg cgctcgcaag agttgacgag gttttgaatg
      841 ccgagccgct cagcatatcg gagcatccgt cacatcnnnn nnnnnngcta cgacggcaaa
      901 aaagacgcac tctcggatat tacgcttaaa ataggcgcct gtgaaaccgt agcttttgtg
      961 ggtccgtcgg gtggcggcaa atccaccctc gcctgcctta ccgcaagatt tttcgatccg
     1021 caaaagggta aagtaatgat aggcggaacc gacatacgcg atattgataa aaaagagctt
     1081 atgaataccg tttcgtttgt tttccaaaac agccgtctta ttaaagcgtc aattcttgaa
     1141 aacgtaaagc tcggcaaacc aaacgcctca aacgaagatg taatgaaagc tctcaaaacc
     1201 gctcaatgca ccgatattat cgagaaattc ccggacgggg tcgatacggt aataggcaca
     1261 aatggagttt atctctcagg cggcgaagca cagcgcatag caatagcaag agcaattttg
     1321 aaagactcac cgatagttat tctcgacgaa gcaacggctt ttgccgaccc ggataacgaa
     1381 gttaaagttc aggcggctat ccgcgagctt tcaaacggca aaacggtaat tatgatagcg
     1441 cacaggcttt ctaccgttgc aaatgccgac aaaatatttg ttttatctga cggcaagctc
     1501 gcagaagagg gcaaatttga cgagctgcgt gaacgcggcg gacttttcag ctctatgtgg
     1561 aacgaatatc aaaaatccgt ttgctggaaa gtatcaaagg aggcataagc tatgataaag
     1621 aaattgcaaa gacgctttgc gctttcaagg aaaggcgccg tggataccgt aaagggcagt
     1681 atattctgtg ctttacaaaa tatttcgttt atgctgccgg ttataatgct ctactatctt
     1741 gtcgacgagc tgataagcaa cagcctcggt cgctcacgca ttccgtttta tgttataggc
     1801 tgtgtaatat gcgccgccgt tatagttata tgcacgattt tacagtataa cggcacattt
     1861 ttcaccacat atgaggaatc cggcgtaagg cgtgtaagcc ttgccgaaca gctccgcaaa
     1921 attcccctgt cattcttcgg gaaaaaggac ctcgccgacc ttacgtcgtc tattatgaac
     1981 gactgcgccg ttcttgaaca aagccagtcg cactttgtgg aaccgctcat aggctcgata
     2041 atatcgacgg tgctgattgc cgtttcgctt ttattcttca at
//
LOCUS       DBMDVO010000197         2073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25808_length_2073_cov_1.079615, whole genome shotgun sequence.
ACCESSION   DBMDVO010000197 DBMDVO010000000
VERSION     DBMDVO010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25808_length_2073_cov_1.079615"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="ACZPE2_03635"
     CDS             <1..394
                     /locus_tag="ACZPE2_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HIR1318973.1"
                     /translation="SEISAEFCIRSSVLLKKEELKSRLALIMRAIGGKVISFGDYPAW
                     EYKKDSRLRSVMTEVYEKMYKKEPVITSIHGGVECGLFSDKIDGLDCVSIGPNLKDIH
                     TYRESMSISSVKRTWEFLLEVLNELAKG"
     gene            complement(440..1564)
                     /locus_tag="ACZPE2_03640"
     CDS             complement(440..1564)
                     /locus_tag="ACZPE2_03640"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR1318974.1"
                     /translation="MSALPCDIDCIAADFSINGNFKEYTALNDGHINSTYVISFDDKG
                     DTVKYTLQKINTGIFKNPDALMSNIVGVTSHIRSALLKAGRDADRGTLTFLKAADGKY
                     YFRDENGDCWRVYKYIDNVYTCNIIDSAEVFENAGRAFGEFQRQLSDYPIDSLFETIP
                     KFHDTYSRFLDFMSAVKEDRAGRAEGVRELIDFAVARENDTHVLLDLIERGSLPLRVT
                     HNDTKLNNILFDRDSNDGICIIDLDTVMPGLSLYDFGDSIRFGANTAAEDEKDVSKVS
                     LSLPLYEAYTRGYLSSAKDSLTETETEYMAFSAKLMTYECGIRFLGDYLNGDVYFKTA
                     VCSCGRYGKENGRNAPYNRKRIKIIRFGNRTNKNSEALLL"
     gene            complement(1660..>2073)
                     /locus_tag="ACZPE2_03645"
     CDS             complement(1660..>2073)
                     /locus_tag="ACZPE2_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318975.1"
                     /translation="CGGKEKDYLPLKRQYVFEFSDIVSAENVRVMSGGEKLDCSVTDA
                     GGRVTVSLPPTEISAPIEAELYGVTVLKNKPKREAVREAMTKFNGINNLKKRRYLILE
                     KAKDDAALLSEVRLLGNSALRSELLEILEDLDYTL"
BASE COUNT          573 a          471 c          453 g          576 t
ORIGIN      
        1 aagtgaaatt tcggcggaat tttgtataag aagcagcgta ctgctgaaga aagaggaact
       61 caagagccgc ctcgctctga ttatgagagc cataggcggc aaggtaattt ccttcggcga
      121 ttaccccgca tgggaatata aaaaagattc gcgcctcaga agcgttatga cagaggttta
      181 tgagaaaatg tataaaaaag aacccgtcat aacctctata cacggcggtg ttgaatgcgg
      241 cttgttttcc gataaaatcg acggccttga ctgcgtttca ataggtccga accttaaaga
      301 catacacacc tatcgcgaga gtatgagcat ttcctctgta aaaagaacct gggagttttt
      361 acttgaggtt ttgaacgagc ttgcaaaggg ttaataacat catcatacca aacagacaac
      421 tataaagcga agccctccgt caaagaagga gagcttcgct gtttttgttt gttcgatttc
      481 caaaacggat tattttaatg cgttttcggt tatacggcgc atttcgtcca ttttcttttc
      541 catatcggcc acaagagcaa actgcggtct tgaaataaac atctccgttt aaatagtcac
      601 cgaggaagcg tataccgcac tcatatgtca taagcttagc cgaaaacgcc atatactcag
      661 tctcggtttc ggtaaggctg tccttggcgg agcttaaata gccccttgta tatgcttcgt
      721 aaagcggcag gctgagcgat acctttgaaa cgtccttttc atcctcagcc gccgtatttg
      781 cgccgaagcg gatgctgtcg ccgaaatcat agagcgaaag cccaggcatt acggtgtcaa
      841 ggtcaattat acagattccg tcatttgaat cacggtcaaa aagaatgttg ttgagctttg
      901 tgtcgttatg agtgacgcga agcggcagac tgccgcgctc tataaggtct aaaagaacgt
      961 gcgtgtcgtt ttctcttgca acggcaaaat caataagctc tcgcacacct tcggctcttc
     1021 ccgctctgtc ctcttttacc gcggacataa aatcaaggaa tctcgagtat gtatcatgga
     1081 acttcggtat tgtttcaaaa aggctgtcaa tgggataatc tgagagctga cgctgaaatt
     1141 cgccgaatgc tctgcctgca ttttcaaaca cctcggcgga gtcgattata ttgcaggtat
     1201 aaacattatc tatatatttg tatacgcgcc agcaatcacc gttttcatcg cggaaataat
     1261 acttaccgtc cgccgcctta aggaacgtta aggttccgcg gtcggcgtct ctgccggcct
     1321 ttaaaagtgc ggagcggata tgtgaggtaa caccgacaat gttgctcata agcgcatctg
     1381 ggtttttaaa tataccggta tttatttttt ggagcgtata ttttacggta tcgcctttgt
     1441 catcaaagga tatgacataa gtggaattta tatgcccgtc attaagagcg gtgtattcct
     1501 taaaatttcc gttaattgaa aaatcagcgg caatacagtc aatatcacaa ggtaatgcag
     1561 acaaattaaa cacgcctttc cgaaaatgct ttttagtaaa gttattttat tatattattt
     1621 aatgaacggt aagtcaacaa aattcgacat ccgcctgttt tacagcgtat agtcaaggtc
     1681 ctccaaaatt tcaagcagtt cggagcgcag cgccgaattg cccaaaagcc gcacctcgga
     1741 aagaagtgcc gcatcgtcct ttgccttttc aaggattaaa taacgacgtt ttttcaagtt
     1801 attgataccg ttgaattttg tcatagcctc acggactgct tcgcgcttcg gtttgttctt
     1861 aagcaccgta acgccgtaaa gctcagcttc tatcggggcg gaaatctccg tgggaggcag
     1921 cgaaacggta actctgccgc ctgcatccgt gaccgaacaa tcaagctttt cgccgcctga
     1981 catgaccctc acgttctctg cggatacaat gtcggaaaac tcaaacacat actgcctttt
     2041 aagcggaaga taatcttttt cctttccgcc gca
//
LOCUS       DBMDVO010000198         2071 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25849_length_2071_cov_1.086802, whole genome shotgun sequence.
ACCESSION   DBMDVO010000198 DBMDVO010000000
VERSION     DBMDVO010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25849_length_2071_cov_1.086802"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="ACZPE2_03650"
     CDS             complement(<1..635)
                     /locus_tag="ACZPE2_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012377.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptM family lipoprotein"
                     /protein_id="HIR1318976.1"
                     /translation="MKRLVCVILSAALLFCLSSCGQKPKIRREIDNSKLLQVSEDGYL
                     TDGSDNGIQLRGVNFGGWLLQETWMCPVMSLDRSLTVKGGTDDGWAELDTLNKFTLLF
                     GEEKTAELFKSYRDNYITEEDFENVKALGFNCIRIPFWYRNFMSDENGTYITENDDEN
                     PGFVKLDFACEMAEKYNLYLIFDMHGCPGGQNGDHSSGKTGRNLLYSDKNY"
     gene            complement(666..1283)
                     /locus_tag="ACZPE2_03655"
     CDS             complement(666..1283)
                     /locus_tag="ACZPE2_03655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1318977.1"
                     /translation="MSSMKDKMHTGELYLPGDEEIMRHQLKRLDRLYDFNMTRPTELK
                     KREEMLREMFAEIGDDCYIEPPFHSNFGGEHVHFGNHIYANFGLTLVDDTHIYVGDNT
                     MFGPHVTVATAGHPIDPTLRAKEYQYNMPIHIGKNCWIGAGALILPGVTIGDNTVIGA
                     GSVVTKDIPSSVVAVGNPCRVMRDVGEHDREYYFKNRKIDYSSIE"
     gene            1393..1812
                     /locus_tag="ACZPE2_03660"
     CDS             1393..1812
                     /locus_tag="ACZPE2_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR1318978.1"
                     /translation="MEYRKFGDTVVARFDRGEEFTSSLQELCEKENIKLASVSAIGAL
                     NAFTAGVYMVDEKRYVKNEFKGCFEIVSLTGTVNTKGGKYYSHLHISAGDESGRVFGG
                     HLNEAHISATAEVVIRIINGEVDRFYDDATGLNLFKF"
BASE COUNT          525 a          457 c          484 g          605 t
ORIGIN      
        1 tgataattct tatcactata caaaagattt ctcccggtct tacccgacga atggtcgccg
       61 ttttgaccgc cgggacagcc gtgcatgtca aatatcagat acaggttgta tttttccgcc
      121 atttcacagg caaaatcaag ctttacaaat cccggatttt cgtcatcgtt ctcggttatg
      181 tatgtgccgt tttcgtccga cataaagttt ctgtaccaga agggtattct gatgcagtta
      241 aatccgagag ctttaacatt ttcaaagtcc tcttcggtga tgtaattgtc acggtagctt
      301 ttaaaaagct ctgcggtttt ttcttccccg aaaagaagcg tgaatttatt taaggtgtca
      361 agttccgccc agccgtcgtc cgttccgccc tttacggtaa gactcctgtc gaggctcata
      421 acggggcaca tccaggtctc ctgcaagagc caacctccga agttgacgcc gcgaagctgt
      481 atgccgttgt cgctgccgtc tgtgaggtag ccgtcctcgc tgacttgcaa gagcttggag
      541 ttgtcgattt cacgccgtat tttcggcttc tgcccgcagg acgaaagaca aaacagcaaa
      601 gccgcagaca gtataacgca aactaacctt ttcataacac cgctcctatt gttaattgtt
      661 ttttattatt cgattgacga gtaatctatt tttctgtttt tgaaatagta ttccctgtcg
      721 tgctcgccga catcacgcat tacgcggcac ggatttccga cggcgaccac agatgacgga
      781 atgtcctttg taacaacgct gcccgcgcct atgacggtgt tatcgcctat cgtaacgccg
      841 ggcagaataa gcgcgcctgc gcctatccag cagttcttgc ctatgtgaat cggcatattg
      901 tattggtatt cctttgctct gagcgtggga tctatcgggt gaccggcggt tgccacggta
      961 acgtgagggc cgaacattgt gttgtcgccg acgtatatgt gagtatcgtc aacaagcgtc
     1021 aagccgaaat tggcatagat atgattgccg aagtgtacat gctctccgcc gaagtttgag
     1081 tgaaaaggag gttctatgta gcagtcatcg cctatttccg caaacatctc cctgagcatt
     1141 tcctcgcgct ttttaagctc ggtcggccga gtcatattaa agtcgtaaag cctgtcgaga
     1201 cgctttaatt gatgccgcat tatctcttca tcgccgggta aatacaattc accggtgtgc
     1261 attttgtctt tcatggagct catttaaatt cctctttctc tcttttattg acataaatta
     1321 taattgatat tatcataagt atatcatttt tgtatacagc tttccactat aaagtttaac
     1381 ggaggaatta caatggaata cagaaaattc ggagataccg tggttgcgag atttgaccgc
     1441 ggagaggaat ttacaagctc gctgcaagag ctctgtgaaa aggaaaatat taagctggcg
     1501 tccgtttcgg cgataggcgc attaaatgcg tttaccgcag gagtatatat ggtggacgag
     1561 aaaagatatg tgaaaaacga atttaaaggc tgcttcgaga tagtttcgct cacgggaacc
     1621 gtaaacacca aaggcggcaa gtattacagc catctgcaca taagcgccgg cgacgagagc
     1681 ggccgtgttt tcggcggaca tcttaatgag gcacatatca gcgcgaccgc cgaggtcgta
     1741 ataaggataa taaacggtga ggttgacagg ttttatgacg atgctacggg ccttaatctg
     1801 tttaaatttt aagccgactc aaattagaat atgcgtcgtg cgctctttgg ggcgttcggc
     1861 gtttttttaa tttctgttga caaatggata atgtgtgtta tactaaatta gttcaattta
     1921 gaactaattg aggtgatgtg atgaccacga tagaatttct gtcggactcg gcaaaactgt
     1981 atcaggatac ggtcttgccc gtctgcaaaa agtatgggct tacgtatatg gaattcggtg
     2041 tattgatgtt tctttacaac aatccgcaat a
//
LOCUS       DBMDVO010000199         2066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25932_length_2066_cov_1.041730, whole genome shotgun sequence.
ACCESSION   DBMDVO010000199 DBMDVO010000000
VERSION     DBMDVO010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25932_length_2066_cov_1.041730"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="ACZPE2_03665"
     CDS             complement(<1..472)
                     /locus_tag="ACZPE2_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318979.1"
                     /translation="MNIKATDFCDASNSAGGVIKAINELQSGDTLIFPKNEYHFYKDR
                     CIHKVCHMTNTDSFKAPDKYFAVLIENKENITVDGCGSTLVIHGDMCAFSLRGCKNVR
                     LANFTVRYASPTNFEMEVVERSLNKITYKIPTGSDFEVKNNKLTFFEKSPFSGEN"
     gene            complement(582..1544)
                     /locus_tag="ACZPE2_03670"
     CDS             complement(582..1544)
                     /locus_tag="ACZPE2_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015818.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR1318980.1"
                     /translation="MQQKTVSKYTKEQTCEFAKEVLFRHYGSRATQIKYIGGGSFGYV
                     YKVELPVMPYTLTVKACRVAGMQHNESAALKKLAESSLVHIPEVYFTFDATDEIPMDF
                     IIEEFVNGTDCFTDFSKLFLSKKRKAKFADAIADCLANWHSVTCNKFGSLDFPEYDNW
                     LDFYRPFAQDILQTAAELNRKKRLKDSTLNVMQTAWKNFDYIFSEPVKEASLIHGDLN
                     VMNVMSDKRLNITAIIDPLECKWADKEFDLFQLRNLTGECFGLYEAYKRKYPVSEKCD
                     LKCAFYAVYHEVYCLISTGTNTEIILAAAVKRLKRELKKAGITS"
     gene            complement(1712..>2066)
                     /locus_tag="ACZPE2_03675"
     CDS             complement(1712..>2066)
                     /locus_tag="ACZPE2_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019886.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1318981.1"
                     /translation="IKKEKSLIFKIAKNVLADRFRKNNFADSLDELESIKCLEVFDDI
                     TSVEIQQILLKMSEIDREIVTLKSYGWSSREIGKIKNMPSSTVRNRLAALKKQIEKEL
                     GEPCGRRKHHNTYKT"
BASE COUNT          530 a          474 c          403 g          659 t
ORIGIN      
        1 aattctctcc gctgaacgga cttttttcaa agaacgtaag cttgttattt ttgacctcaa
       61 agtcgcttcc cgtcgggatt ttatatgtaa ttttattgag cgaacgctct acgacctcca
      121 tttcaaaatt tgtcggggag gcatatctta cggtgaaatt cgcaagcctt acatttttac
      181 agccgcgcag agaaaacgca cacatatcgc cgtgaatcac aagggtagaa ccgcagccgt
      241 ccacggtaat attctcctta ttctcgataa gcaccgcaaa atacttgtcg ggtgccttaa
      301 aggagtccgt atttgtcata tggcaaacct tgtgaataca gcggtccttg taaaaatgat
      361 actcattctt cgggaaaata agagtgtctc cgctctgtaa ttcatttatc gcttttataa
      421 cgcctccggc agaatttgag gcgtcgcaga aatccgtggc tttgatattc aatgtcatcc
      481 tccgattgta aattatagca taagctgttt agatggtttt taactatatg ctcgttttcg
      541 gcacattcag gtgtgctcgc atcaccgttt gagtcggatt ttcaagatgt aatacccgct
      601 tttttaagct ccctcttcag tctcttgact gccgctgcaa gtatgatttc ggtgtttgtt
      661 ccggttgaaa taaggcaata cacctcgtga tacaccgcat aaaacgcgca cttcaaatca
      721 cacttttccg aaacgggata tttcctttta tatgcttcgt aaagcccgaa gcactccccg
      781 gtcaggtttc tcagctggaa caaatcaaat tctttgtcag cccacttgca ctcaagaggg
      841 tcgattatcg cggtgatatt aagtctttta tcggacatta cattcataac attcaaatcg
      901 ccgtgtataa gagacgcctc ttttacgggc tcgctgaaaa tgtaatcaaa atttttccac
      961 gctgtttgca taacattaag cgtgctgtcc ttcagccgtt tttttctgtt aagctctgcg
     1021 gcggtctgca aaatatcctg cgcaaacggt cggtaaaaat caagccaatt atcgtactcg
     1081 gggaaatcga ggctgccgaa tttgttgcag gtaacgctgt gccaatttgc aagacaatcc
     1141 gctattgcat ctgcaaattt cgcttttctt tttttgctta aaaacaattt tgaaaagtcg
     1201 gtgaagcagt ctgttccgtt cacaaattcc tcaattataa aatccatggg gatttcgtcg
     1261 gttgcgtcaa aggtaaaata gacctcggga atatgaacca gcgagctttc ggcaagcttt
     1321 ttcagcgcgg cgctctcatt atgctgcatg ccggcgacgc ggcaggcctt taccgtaaga
     1381 gtgtacggca tcacaggcaa ttcgaccttg taaacatatc cgaaggagcc gccgcctatg
     1441 tatttaatct gcgttgctcg gctcccgtaa tgtctgaaaa gcacttcctt tgcaaattcg
     1501 catgtctgtt cttttgtgta tttgctgact gttttttgct gcataggtac ctcccctttt
     1561 tcacactgtt tttcaatttt gttaataccc gtttgttttc gacgtaccga tttacacatt
     1621 tatgttttaa agatataata tcaaaaaaaa taataaatat caataaacat ctctgtacaa
     1681 aagagccgtc ggacacatag cccgacagct cttaggtctt atatgtatta tggtgctttc
     1741 gacgtccgca aggctcaccg agctcctttt caatttgctt tttaagcgca gccagcctgt
     1801 ttctgaccgt tgacgacggc atatttttta tcttgccgat ctctctgctc gaccagccgt
     1861 agcttttaag agtcactatt tctcggtcga tctcgctcat tttcaaaagt atctgctgaa
     1921 tttcaacaga tgtgatatca tcaaacacct caagacattt aatgctttca agctcatcaa
     1981 gcgagtcggc aaaattgttt ttacgaaatc tgtcggcaag cacgttttta gcaattttaa
     2041 aaatcaagga cttttctttt ttgatg
//
LOCUS       DBMDVO010000200         2066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_25933_length_2066_cov_1.035115, whole genome shotgun sequence.
ACCESSION   DBMDVO010000200 DBMDVO010000000
VERSION     DBMDVO010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25933_length_2066_cov_1.035115"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="ACZPE2_03680"
     CDS             <1..196
                     /locus_tag="ACZPE2_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318982.1"
                     /translation="PIYMILTSFLLVFLDAGASQISMVFNLNESFSDILTGIILFFII
                     GSEFFINYELKFRKTKKEVK"
     gene            198..1187
                     /locus_tag="ACZPE2_03685"
     CDS             198..1187
                     /locus_tag="ACZPE2_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1318983.1"
                     /translation="MFSLITFLHRAIMQGIPLLFGSTGEIMTEKSGNLNLGIPGVMYI
                     GGMSGVIGAFLYENSLSSPSDAVGFLAVMIPLLCCALGSLLAGLIYCFLTVTLRANQN
                     VTGLALTTFGTGFGNFFGGSLIKLVGSDVPSVALTTTSNYFRTTLPGAETTGWFGKLF
                     LSYGFLAYAAVIIAIICSYFLNHTRHGLNLRAVGENPATADAAGINVNRAKYLSTCIG
                     SVIAGFGGLYYVMDYANGVWSNDGFGDRGWLAIALVIFAIWRPNLSILGSIVFGGLYI
                     VYLYVPGALRTQELYKMIPYVVTIIVLILVSVRDKKENQGPASLGLSYFREDR"
     gene            complement(1410..1694)
                     /locus_tag="ACZPE2_03690"
     CDS             complement(1410..1694)
                     /locus_tag="ACZPE2_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR1318984.1"
                     /translation="MDAWQYILVYYISISIPSVIITVYDKIAAIKKAVRIPERTLMTF
                     AALGGAAAMYVTMKAIRHKTLHKKFMIGLPVFIILHTLIIGLSYYFLITK"
     gene            1759..>2066
                     /locus_tag="ACZPE2_03695"
     CDS             1759..>2066
                     /locus_tag="ACZPE2_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318985.1"
                     /translation="MFKKVTVILFVLLLLSSCSLKNINLTEPESASFDSGNDNCPNTD
                     DIIADEYRGVWINYNELSMKSVGGGTAEDFKNKIDTMFENIKAFGLNTVIFQVRPFSD
                     "
BASE COUNT          601 a          376 c          409 g          680 t
ORIGIN      
        1 tccgatatat atgattttga catcattcct acttgttttc ctcgatgcgg gtgccagtca
       61 gatttcaatg gtgtttaatc tcaatgaatc attttctgat atattgaccg gtataattct
      121 gttctttatc ataggtagtg aattctttat taactacgaa ttgaagttta gaaaaacaaa
      181 aaaggaggta aagtaaaatg ttttcattga ttacttttct ccatagagct ataatgcagg
      241 gtataccgct cctttttggt tcgacgggcg aaattatgac tgaaaagtcc ggaaacctta
      301 atctcggtat cccgggtgtt atgtacatcg gcggtatgag cggtgttata ggtgcgtttc
      361 tttatgaaaa ttcgctctcg tctccatccg atgcagtcgg atttcttgcg gttatgattc
      421 cgcttttgtg ctgcgctttg ggttctttac tcgcaggttt gatttattgt ttcctcacag
      481 ttactttacg agccaatcag aatgtaacgg gtcttgcact tactactttt ggtacgggct
      541 tcggtaactt tttcggagga tctcttatta aacttgtagg ttcagacgtt ccgtcggtag
      601 cacttacaac aacaagcaat tacttcagaa caacattacc gggagcagaa accaccggat
      661 ggttcggtaa gctgttttta tcgtacggat ttttggcata tgcagctgta ataatagcaa
      721 ttatctgttc atatttcctg aatcataccc gtcacggatt aaatcttcgt gcggtaggtg
      781 aaaatcctgc aacagcagat gctgcaggaa taaacgtaaa cagagccaaa tatctctcta
      841 cctgtatagg aagtgttatt gcgggcttcg gaggtcttta ttatgtaatg gattacgcca
      901 acggcgtttg gtcaaatgac ggcttcggcg acagaggctg gcttgcaatt gcgcttgtta
      961 tattcgctat ttggcgtccg aatctcagca tactcggttc tatagtattc ggcggactct
     1021 atattgttta tctttatgtt ccgggtgcac tccgcacaca ggagctttac aagatgatac
     1081 catatgtagt cacaataatt gttcttattc ttgtaagtgt aagagataag aaagaaaacc
     1141 agggaccggc atcactcgga ctttcatatt tcagagagga ccgataattc aagtaattat
     1201 actttctaaa ccgtctgttg ttttaaaaag acggattaca gcaaaatatt aatcaaaaaa
     1261 agacagcata aagagtaatt tttactctct gtgctgtctt ttgctatctg aaaaaatatt
     1321 ttatttgcat cttttaattt aagtctgttt acggcctaga aaaaacaaaa aaattaagtt
     1381 gcatcaacat ggtttttacc aaatctttac tattttgtta ttaaaaagta atacgaaaga
     1441 cctataatca atgtatgtaa aataataaat accggtaaac ctatcataaa ttttttgtga
     1501 agagttttat gccttattgc cttcatagtt acatacattg cggcagctcc tccgagggcg
     1561 gcaaaagtca taagagtcct ttcgggaatt ctcacggctt tttttattgc agctattttg
     1621 tcgtaaacgg ttataattac cgacgggatt gaaatagaaa tgtagtagac aagtatgtat
     1681 tgccaagcgt ccataatatc accgttacta taatataatt taatcatttg cttgtcaatg
     1741 cgaaaaggaa ggctttaaat gtttaaaaaa gttactgtta ttttatttgt gttgctgctt
     1801 ctatcgtcat gcagcctgaa gaacataaat cttactgaac ccgaaagcgc atcatttgac
     1861 agcggtaacg acaactgccc taacaccgat gatattatcg ctgatgaata tcgcggcgtg
     1921 tggataaact acaatgaatt gtcaatgaaa tcagtcggtg gcggaactgc cgaagacttt
     1981 aaaaataaga tcgatacaat gtttgaaaat atcaaagcat tcgggcttaa tactgttatt
     2041 tttcaggtca gacccttttc tgacag
//
LOCUS       DBMDVO010000201         2058 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26090_length_2058_cov_0.837506, whole genome shotgun sequence.
ACCESSION   DBMDVO010000201 DBMDVO010000000
VERSION     DBMDVO010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26090_length_2058_cov_0.837506"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="ACZPE2_03700"
     CDS             <1..626
                     /locus_tag="ACZPE2_03700"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1318986.1"
                     /translation="KLDLIDDDKDCLTNINRQIIATRSTIGKYKVEVAEERIKDINPD
                     CEVTTHRKFYTPETSAEFDFSQYDYVVDAIDTVSGKIELVMQAQKSQTPIICSMGAGN
                     KMDPTAFEVTDIYKTSVCPLARVMRYELKKRGVKKLKVVYSKEKPLVPIADTAISCRE
                     HCICPPGTARNSTQRRAIPGSNAFVPPVVGLIIAGEVVKDLTDYRAK"
     gene            649..1194
                     /locus_tag="ACZPE2_03705"
     CDS             649..1194
                     /locus_tag="ACZPE2_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1318987.1"
                     /translation="MTRDEAYALLTEYNKEPFHLEHAETVEKTMMYFADKLGYGDDRD
                     FWGIVGLLHDLDFEMYPEEHCIKEQEIMCERGIDEKIIHATASHGYGITVDIKPDHEM
                     EKVLYAVDELTGLIGAVVKMHPSHSVQDLELKSVKKKFKAKNFAAGCSREVIQRGADM
                     LGISLDELIQNTIDALKSFQD"
     gene            complement(1292..1996)
                     /locus_tag="ACZPE2_03710"
     CDS             complement(1292..1996)
                     /locus_tag="ACZPE2_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HIR1318988.1"
                     /translation="MSWTLYWVLLAIGIVMTGIFLFLRVKKGGISALYAKAAASLCFI
                     STAFAATSANRDNLQFGLWVAFGLILGMVGDIWLDLKWIYLQDKDSYLYSGFISFLLG
                     HICFNVAIYKSYRFTGKTLAVSIGVSLLIALGNILTEKLMKNHFGKFKFIVFLYTFFL
                     ALTMVSAIIAAIATGFEKVWVVMAVGGVLFTLSDAVLSGMYFAENKNTKFNIFLNHIL
                     YYAAQFTMAATILCIK"
BASE COUNT          670 a          430 c          465 g          493 t
ORIGIN      
        1 gtaaattaga ccttatagac gatgacaagg actgccttac gaatataaac cgtcagataa
       61 tcgcaacgcg ttcaacaata ggaaaatata aggtcgaggt tgccgaagaa cgcatcaagg
      121 acataaatcc ggactgcgag gttacaacgc atcggaagtt ttacactccg gaaacatcgg
      181 cggaatttga tttttcacag tacgactatg ttgttgacgc catagatacc gtatcgggca
      241 aaatcgagct tgttatgcag gcacaaaaga gccaaactcc cataatctgc tcaatgggtg
      301 ccggcaacaa aatggacccg actgcatttg aggtgactga catttataag acttcggtct
      361 gtccgcttgc gagagttatg cgatacgagc tgaaaaaacg cggagttaaa aaactcaagg
      421 tagtttattc aaaggaaaag ccgcttgtac caattgcgga tacggcgata agctgccgcg
      481 agcactgcat ttgccctccc ggtacggcga gaaattccac acaaagacgt gctatccccg
      541 gcagcaatgc gtttgtcccg ccggtagtcg gactgataat agcaggtgaa gtcgtaaaag
      601 atttgacgga ttacagggca aaataaatac acaaaagggt gtttgaatat gacaagagac
      661 gaagcatacg cgctccttac ggaatacaac aaggagccgt ttcatcttga gcacgccgaa
      721 acggttgaaa aaacaatgat gtattttgcc gacaagctcg gctacggcga cgacagggat
      781 ttttggggaa tcgtaggtct tttacatgac cttgacttcg aaatgtatcc cgaagagcac
      841 tgcataaagg aacaggaaat aatgtgtgaa agaggcattg acgaaaagat aattcacgca
      901 acggcaagcc acggctacgg cataacggta gacattaagc cggatcacga gatggaaaag
      961 gtcctttatg cggtagatga gcttacgggg cttataggag ccgttgtaaa aatgcaccct
     1021 tcgcacagcg tacaggatct tgagcttaaa tccgtgaaga aaaaattcaa ggcgaagaat
     1081 tttgccgccg gctgctctcg cgaggtcata cagcgcggtg cggatatgct cggtatttcg
     1141 cttgatgagc tgatacaaaa tacgattgac gccttaaagt catttcagga ctgaagcatt
     1201 aaaaatataa taattaaagc ttagttattt tttaactaaa ggcggatata aaaatacggt
     1261 gggaactaaa tttgagctcc caccgttttt tttatttaat gcaaaggatt gtcgccgcca
     1321 tcgtaaactg agctgcgtag tataagatat ggtttaagaa tatgttaaac tttgtattct
     1381 tgttttcggc aaagtacata cccgaaagca cggcgtcgga cagagtaaag aggacaccgc
     1441 ctacagccat aacaacccaa accttttcaa atccggtggc aattgccgct ataatggccg
     1501 aaaccatagt caacgccagg aagaaggtat agagaaatac aataaattta aacttgccga
     1561 aatgattttt cataagcttt tcggtcaata tattgccgag agctatgaga agcgatacgc
     1621 ctatggaaac tgcaagtgtt ttgccggtaa atctgtagct tttataaatc gccacgttaa
     1681 aacagatgtg acctaaaagg aatgagataa agccggaata aagatatgaa tccttatcct
     1741 gcaaataaat ccatttcaag tcaagccaaa tatcgccgac cattccgagt ataagtccaa
     1801 acgccaccca aaggccgaat tgaagattgt cgcggtttgc gcttgttgcg gcaaaagcgg
     1861 ttgaaataaa acagagactc gccgccgcct tggcatacag tgcggaaatt ccgccctttt
     1921 taacgcgcaa gaacaaaaat atgcctgtca taacaatgcc gattgccaaa agcacccaat
     1981 ataatgtcca gctcatagtt atcccccaaa tttaatttct gtttaatcaa tgagcagatt
     2041 atataatatt tcaacaat
//
LOCUS       DBMDVO010000202         2054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26157_length_2054_cov_1.248336, whole genome shotgun sequence.
ACCESSION   DBMDVO010000202 DBMDVO010000000
VERSION     DBMDVO010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26157_length_2054_cov_1.248336"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(245..>805)
                     /locus_tag="ACZPE2_03715"
     CDS             complement(245..>805)
                     /locus_tag="ACZPE2_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024919.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR1318989.1"
                     /translation="CWAPVIENAVNKFIGKNYTVKTIYGSSIDELCSKYIDNDIPVLV
                     WATQNMAPARRYKTWYITGTSDQFTWITPMHCLLLVGYDDSGYYFNDSLQCKNMRYDK
                     SKVISAYNALNCQAVVIAPKSASDEPPSSTKPETTTSPATTKPQNTTDPSAATKPDTT
                     DSHGTTSATTAAEKDTASPLKNNEHN"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<816..1329)
                     /locus_tag="ACZPE2_03720"
     CDS             complement(<816..1329)
                     /locus_tag="ACZPE2_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024919.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR1318990.1"
                     /translation="MNFKCGKKKNVIIIIIGIVTVAICITAAVLVSRAKKKAAAAQFV
                     PETETYSEESEEALIEEETVSETASETAPIVKPVGTTAPVERAAPAIPKKLTQPKPSI
                     TPTKLLNVPFIDQRKKYPTGCESISAVMALQYLGISISPETFIDNYLDKGRTPYSDES
                     GNLFGDDPRKV"
     gene            complement(1348..1965)
                     /locus_tag="ACZPE2_03725"
     CDS             complement(1348..1965)
                     /locus_tag="ACZPE2_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1318991.1"
                     /translation="MNIEIADRLVKLRKEHNLSQEALASKLGLSRQAVSKWERAEASP
                     DTDNLVALAELYGVPLDEILNGPKAQEENTPNNEPTKAELKEKKLADMSKRQRVGRKM
                     VKFPFPILIAAAYLLLGFINGNWHPGWIILLLIPVYYCVGGALCCRTKKAMLLTMPIH
                     LVLVAVYLLFGFTAGMWHPQWLIFLIIPLYYWIISVFYKEKKNKS"
BASE COUNT          461 a          466 c          486 g          631 t
ORIGIN      
        1 aacgagagaa tgaatatccc gaagggcttt acccagatta aggaagaaga tattccgact
       61 atcgtatcaa gggctcttaa agaggcaaat ccgctctatc ccgttccggt tatctttgac
      121 gaggacagaa tgactcggat agtcaagaag cttatgatta ccgaataagc gataactcaa
      181 agtaaaaaaa tcagcgagtg tattttggct aaacccaaat acactcgctt ttttatgctt
      241 ttgtttaatt gtgctcgtta ttctttaagg gagacgcggt atctttttcc gcagcggttg
      301 tggccgatgt tgtgccgtga ctgtccgttg tgtccggctt tgttgcggct gacgggtcgg
      361 tcgtattctg cggctttgtt gttgcaggcg atgttgttgt ttcgggcttt gtgctgcttg
      421 gcggttcatc ggatgccgat ttcggtgcaa ttaccaccgc ttgacagttc agcgcgttgt
      481 atgcggatat aaccttactc ttgtcataac gcatattttt acattgtaac gaatcgttaa
      541 agtagtaccc gctgtcatcg tagcccacaa gaagcaggca gtgcatggga gttatccacg
      601 tgaattggtc ggatgtaccc gtgatatacc aagtcttgta tcttcttgcc ggagccatat
      661 tttgcgtagc ccatacaagc accggaatgt cgttatctat atattttgag catagctcgt
      721 ctatggagct gccgtatatt gtttttacgg tgtaattttt gccgatgaat ttattgactg
      781 cgttctcaat taccggtgcc caacannnnn nnnnnacacc ttgcgcggat cgtctccgaa
      841 taaatttccg ctttcgtcgc tgtacggtgt gcgccctttg tcgagataat tgtctataaa
      901 tgtttcgggc gaaatagata taccgaggta ttgaagcgcc ataaccgcag atatgctttc
      961 acagcctgtg ggatattttt ttctctggtc gataaacggc acgtttaaaa gctttgtggg
     1021 tgttatgctc ggcttcggct gcgtcagttt tttcggtatc gccggagcgg ctcgctctac
     1081 cggagctgtt gtgccgacgg gctttacaat cggagctgtt tcggatgctg tctccgacac
     1141 ggtttcttcc tcaatgagag cttcttccga ttcttccgag taagtctcgg tttcgggcac
     1201 aaattgcgct gccgccgctt ttttctttgc tctcgataca agcactgcgg cggttataca
     1261 tatcgcaacc gtaacgatac cgattataat aataattaca tttttctttt taccgcattt
     1321 gaaattcact tttttagccc cttaaaatta tgatttgttt ttcttttcct tgtaaaacac
     1381 gctgatgatc caataataca gcggaattat aaggaaaata agccattgcg gatgccacat
     1441 tcccgcggta aagccaaaga gcaggtacac cgcgaccaat acaagatgta tcggcatagt
     1501 gagaagcatc gcctttttgg ttcggcagca aagagcaccg ccgacgcagt agtaaacagg
     1561 tataagcagc agtattatcc agccgggatg ccagttaccg ttaataaagc cgagaagcag
     1621 atatgctgcg gcaattaaaa tcggaaacgg gaattttacc attttgcggc cgaccctttg
     1681 tctcttggac atatcggcaa gcttcttttc tttaagctct gcctttgtcg gctcattgtt
     1741 cggcgtattc tcctcctgcg cttttgggcc gttgagaatt tcgtcaagcg gcacgccgta
     1801 aagctctgcg agagcgacta agttatccgt gtcgggcgac gcctccgcgc gctcccattt
     1861 cgaaacagcc tggcggctga gtccgagctt tgaggcaagt gcttcctgtg agaggttatg
     1921 ctccttgcga agcttaacga gtctgtcggc tatttctata ttcatctgtt atctcctgtt
     1981 cgtgaatgta ttattttgct gtgtagccca ttcttgtgca gttctctata tgctcatcgt
     2041 atgacgccga gact
//
LOCUS       DBMDVO010000203         2040 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26397_length_2040_cov_1.360495, whole genome shotgun sequence.
ACCESSION   DBMDVO010000203 DBMDVO010000000
VERSION     DBMDVO010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26397_length_2040_cov_1.360495"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            194..1531
                     /locus_tag="ACZPE2_03730"
     CDS             194..1531
                     /locus_tag="ACZPE2_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016289.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1318992.1"
                     /translation="MKEENRRYAEEAFEIVEHAAKKIGSRLPGSDGEKEYAAYMGDKL
                     RSIGIEPVREEFAVSPRASIGGIPYAGYLGLIMSALVYVALHLSAVWYGMALASVVCW
                     VWLILSVFLYKTWFDMFFKQEISQNTYGELLPPDGKYDYTIILSGHTDTSWCWRHSAH
                     ASKFKKTKPAMGLVATFAKVGFGVICFLFLTAVSIAMTVISSGVYFGEVWALKTANSA
                     SFANFLFAMHFLPVVTAIGCMFVVMWADPNPRNASRGAMDNATGCALSFAVTKYFHDH
                     PEKMPKNCRIIDFNCGSEEAGLRGSIAFTREHKGEPILENTWNINIDSVADKDFFEVV
                     IKDDWQFCRFDTDLEKMFKDTFNELGIVSKTTGCIHNPVGGCDSTPMTKAGMKSVTFA
                     AQDPTLTYYYHTWHDMPERFELETVGDGFDVILGVIDKIDKFQQANGFNGPQK"
     gene            1674..>2040
                     /locus_tag="ACZPE2_03735"
     CDS             1674..>2040
                     /locus_tag="ACZPE2_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1318993.1"
                     /translation="MLTLNKVYHASFVLKNIIRKTDMILAPNINPESEIHLKTENLQV
                     TGSFKVRGAAYKISQLSEEEKARGVIACSAGNHAQGVALAATKNGIKSLICLPDGAPI
                     SKVEATRKYGADICLVEGVY"
BASE COUNT          549 a          429 c          464 g          598 t
ORIGIN      
        1 tttactaaac aattattttg ccgattgtca attaacaaaa gtgaatttcg gttcaccttt
       61 aaacacaaaa ttcattttgc gtcaaaacag gtgtttttta cgccggtttt agctgttttt
      121 tgaattttgc acttgcattt attgcataaa tgcaatatat ttatttatgt attttatttg
      181 ggaggtttat tatatgaaag aagaaaacag aaggtatgcc gaggaagcgt tcgaaatagt
      241 cgagcacgct gccaaaaaaa tcggctcacg tctgcccggc tccgacggcg aaaaagagta
      301 cgcggcttat atgggagata agctcagaag tatcggtatt gagcctgtac gtgaggaatt
      361 tgccgtatct ccgagagcgt cgataggcgg cattccgtat gcaggttatc tcggacttat
      421 tatgagcgct cttgtttatg tggctctgca tttgagcgcg gtttggtacg gtatggcgct
      481 tgcttcggtt gtatgctggg tttggcttat tttaagcgtt tttctttata agacatggtt
      541 cgatatgttc tttaagcagg aaatatcaca gaacacttac ggtgagcttt taccgcccga
      601 cggaaaatac gactacacca taattctttc gggacacacg gatacaagct ggtgctggag
      661 acattccgct cacgcttcaa agtttaaaaa gacaaagcct gccatgggtc ttgttgcaac
      721 cttcgccaag gttggcttcg gcgttatatg cttccttttc ctcactgccg tttcaatagc
      781 catgaccgtt atttcaagcg gagtgtattt cggtgaggtt tgggctttaa agaccgccaa
      841 ttccgcctcc tttgccaact tcctttttgc tatgcacttc cttcccgttg ttacggcaat
      901 aggctgtatg tttgtagtta tgtgggctga cccgaatccg cgcaacgctt caagaggtgc
      961 tatggataac gccaccggtt gtgctctcag ctttgccgtt acaaaatact tccatgatca
     1021 tcccgaaaag atgccgaaga actgccgtat aatcgacttc aactgcggct ccgaggaagc
     1081 gggacttcgc ggttctatcg catttacccg tgagcataag ggagagccga tacttgaaaa
     1141 cacatggaac atcaatatcg actccgttgc agataaagat ttctttgaag ttgttattaa
     1201 ggatgattgg cagttctgcc gctttgatac cgaccttgaa aaaatgttta aggatacctt
     1261 taacgagctc ggtatagtca gcaagacaac cggctgcata cataaccctg taggcggatg
     1321 cgacagcact cctatgacaa aggcaggtat gaaatcggtt acctttgcgg ctcaggaccc
     1381 cacacttacc tattattatc atacctggca cgatatgccc gaacgttttg agctcgaaac
     1441 cgtcggtgac ggctttgatg ttattctcgg agttatcgat aaaatagata agttccagca
     1501 ggcaaacggt tttaacggac ctcagaaata attaaacttt ttatgtcccc gaagcattgg
     1561 ctcagccttg tcttcgggga ttattttttg ccggagcgtt aaaacctttt tattgatttt
     1621 tgaccgtaac gtgatatact aatatatact attttttgaa agaagagata aagatgttaa
     1681 cactcaacaa agtctatcac gcttcatttg ttttaaaaaa cataattcgt aaaacggata
     1741 tgattttggc gccgaacata aatcccgaaa gtgaaataca tctcaagacc gaaaatttac
     1801 aggttacagg ttcgtttaag gtcagaggtg ccgcatataa aatatcacag ctgtctgaag
     1861 aggaaaaggc acgcggcgta attgcctgct cggcaggtaa ccacgcgcag ggcgttgctt
     1921 tggcagctac caaaaacggt attaaatcac ttatctgcct gccggacggt gctcccatat
     1981 caaaggttga ggcgaccaga aaatacggcg cggatatttg tcttgtagag ggcgtttatg
//
LOCUS       DBMDVO010000204         2038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26435_length_2038_cov_1.027878, whole genome shotgun sequence.
ACCESSION   DBMDVO010000204 DBMDVO010000000
VERSION     DBMDVO010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26435_length_2038_cov_1.027878"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="ACZPE2_03740"
     CDS             <1..444
                     /locus_tag="ACZPE2_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012974.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HIR1318994.1"
                     /translation="FYREVRDSVINQGDKINKVNTQRTKALRAAKAEEILIASFMSDP
                     ELYRKLESKLTPELFVTDFNRRVFSAISERIKDGRPVGLSFFSSEFTPEEISVIARIE
                     TVSTDISNSVRECEDCINVLKKEKNRHIDVKPSEMSDEDFMKLFK"
     gene            474..1604
                     /gene="rpoD"
                     /locus_tag="ACZPE2_03745"
     CDS             474..1604
                     /gene="rpoD"
                     /locus_tag="ACZPE2_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1318995.1"
                     /translation="MDNNTNHTTAAAAGEKKNSVKALLEKGKSTGKLTTQEIDAAIVE
                     EDFDMEELDKLYETIESMNIEIIDDFGDVELDNLNFDTELPKSQDAAAAASADTHNLA
                     MDDPVKVYLKEIGRVPLLTAEEEQVLAMRIADGDQQAKQRLAEANLRLVVSIAKRYVG
                     RGMQFLDLIQEGNLGLIKAVDKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKVKKTNSQLLHKNGRDPTAEEIADELQMPVEKVREILRVAQEPVSLETPIG
                     EEEDSHLGDFIPDDDALAPADAASMLLLKEQLGEVLKTLTAREEKVLSLRFGLEDGHP
                     RTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD"
     gene            1811..>2038
                     /locus_tag="ACZPE2_03750"
     CDS             1811..>2038
                     /locus_tag="ACZPE2_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492077.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1318996.1"
                     /translation="MKYKKNDILKYTGKYADICRETGYIDPVLYDEYGVKRGLRDKNG
                     NGVLAGLTSISKIKAFEDTPDGRIPCPGELYY"
BASE COUNT          646 a          415 c          493 g          484 t
ORIGIN      
        1 ttttatcgcg aagtgcgtga ttcggttata aatcaaggcg ataagataaa taaggtcaac
       61 acacagcgca caaaggctct tcgggcagca aaagcagagg agatactcat agcgtcgttt
      121 atgtctgacc cggagcttta ccgaaagctt gaaagcaaac tgactccgga gcttttcgtt
      181 accgatttca accgacgtgt tttttcggca atatcggagc gaattaaaga cggaagaccg
      241 gttgggttat cttttttttc atcggagttt actccggagg agataagcgt tattgcgcga
      301 atagaaactg tttcaaccga tatttccaat tctgtcaggg aatgtgagga ttgtataaac
      361 gtcctcaaga aagaaaagaa cagacatatc gatgtgaaac cgtctgaaat gtccgatgag
      421 gactttatga agctgtttaa ataaaaaaat acatagatgt gtgagggaaa aatatggaca
      481 acaacacaaa ccacaccact gccgcggcag cgggcgaaaa gaaaaactcc gtaaaagcat
      541 tgcttgaaaa gggtaagtcg accggcaagc tcactacgca ggaaattgac gccgcgatag
      601 ttgaggaaga tttcgatatg gaagagcttg ataagctcta cgaaaccatc gaaagcatga
      661 atatagaaat cattgatgat ttcggcgatg tcgaattgga caatctgaac tttgataccg
      721 aattgcctaa gagtcaagat gccgctgcgg cagcaagtgc cgatacgcat aatcttgcca
      781 tggatgatcc ggtcaaggtg tatcttaagg aaatagggcg tgttccgctt cttaccgctg
      841 aggaggagca ggtgctcgca atgcgtatag ccgacgggga tcagcaggct aaacagcgtc
      901 ttgccgaagc aaaccttcgt cttgtagtca gcattgccaa acgttatgtc ggcagaggta
      961 tgcagttcct tgatttgatt caggagggca accttggcct tataaaggcg gttgataaat
     1021 tcgactacac taagggcttt aagttctcca cctatgccac atggtggatt cgtcaggcta
     1081 taacaagagc gatagccgat caggcaagaa ccataagaat tcccgttcat atggttgaga
     1141 caatcaacaa agttaaaaag acaaacagcc agcttctcca taagaacggt agagacccga
     1201 cggcagaaga aatagcagat gagcttcaga tgcctgtgga gaaggtacgt gaaatcctca
     1261 gagtcgcaca ggagcccgtt tctcttgaaa ccccgatagg cgaagaggaa gacagccatc
     1321 tcggagactt cattccggat gacgacgctc tggcacctgc agacgctgcg tctatgctgc
     1381 ttttaaaaga acagctcggt gaggttctca aaacacttac cgcacgtgaa gaaaaggtgc
     1441 tttcgcttcg tttcggactt gaggacggac atccgagaac acttgaggaa gtcggcaagg
     1501 aatttaatgt aacaagagag cgtattcgtc agatagaggc aaaggctctc cgtaagctcc
     1561 gccacccgag ccgcagcaaa aagctcaagg atttcctcga ctgataaaac cgcaaaaagc
     1621 ttccccgaat gccattctgt cgcattcggg gatttttttg ttaataaatt cttaatttta
     1681 tgttaattga caagtgttaa ttaaattggt ataatataaa atattagata gaaatacttt
     1741 acatataaat gtaaaaatca ggtataatac cggcacgatt tcagcttacc gccggaaatg
     1801 aggagagaaa atgaaataca aaaagaacga tattctgaag tataccggaa aatatgcaga
     1861 tatttgcaga gaaacgggtt atatagaccc ggtgctttat gatgaatacg gcgtaaaacg
     1921 cggacttcgc gataagaacg gaaacggcgt tttggccgga cttacttcaa tatctaaaat
     1981 caaagccttt gaggatacgc ccgacggaag aatcccgtgt ccgggtgaac tttattac
//
LOCUS       DBMDVO010000205         2031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26581_length_2031_cov_1.198964, whole genome shotgun sequence.
ACCESSION   DBMDVO010000205 DBMDVO010000000
VERSION     DBMDVO010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26581_length_2031_cov_1.198964"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="ACZPE2_03755"
     CDS             <1..406
                     /locus_tag="ACZPE2_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1318997.1"
                     /translation="ELMQARKIASERKEILTAAQSTAEGIITAAKKKADELISEHEIT
                     KGAEAEAENIMAEARKKANGIVEQARGTAAELTEQAQKWSNDMRKSAGEYVERVVSMA
                     DEGLTNSVNEIRRVRQSLKAAAQHKKAPEESK"
     gene            473..958
                     /locus_tag="ACZPE2_03760"
     CDS             473..958
                     /locus_tag="ACZPE2_03760"
                     /inference="COORDINATES: protein motif:HMM:NF027438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HIR1318998.1"
                     /translation="MFVYSVKKRQVKMALLVFFVALLAVALIIVSHHTAEAGKTAALN
                     IRAASSEDRIAYLSQFGWSVKEDPVEVCEVIIPAEFDDVYEKYNELQKEQGFDLSLYA
                     SKRVKRWTYEITNYTGYENTGFIHANILVYDGKVIGGDVCSTELNGFMHGFAKPDNTG
                     E"
     gene            973..1686
                     /locus_tag="ACZPE2_03765"
     CDS             973..1686
                     /locus_tag="ACZPE2_03765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1318999.1"
                     /translation="MKKERIDKLIAGSGKLSRKDVAGLISHGNVTLNGRTVKSGSEKA
                     DADADEIKVCGEIFTIKKHIYIMLNKPKGVVSASDGKGERTVCDLVPEKLMRRGLFPA
                     GRLDKDTTGFVLITDDGEFAHRILSPKNHIDKTYIVSLRDNIEEEAVSLLENGITLRD
                     GTAFLPSKIKILSDKECEITICEGKYHQIKRMFCAVGNEVTNLRRIKMGRLFLDERLR
                     DGECREITADELKMISEKE"
     gene            complement(1786..>2031)
                     /locus_tag="ACZPE2_03770"
     CDS             complement(1786..>2031)
                     /locus_tag="ACZPE2_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR1319000.1"
                     /translation="LYTDSKYVADAITKGWAEGWKRKGWKKSDGKPALNSDLWDELLT
                     LISKHSLNIVWLKGHAGHPENERCDSMAVRESHKFIK"
BASE COUNT          595 a          429 c          510 g          497 t
ORIGIN      
        1 tgagcttatg caggcgagaa aaatagcctc cgagagaaag gaaatactta ctgcggcgca
       61 aagcaccgcc gaggggatta ttaccgcggc aaagaaaaag gccgatgagc ttatttccga
      121 gcacgagata acgaagggcg ccgaggccga ggctgaaaat attatggcgg aagcacgtaa
      181 aaaggcaaac ggtatcgtcg agcaggcgag agggactgca gcggagctta cagagcaggc
      241 tcaaaagtgg tcaaacgata tgcgtaaaag cgcaggcgaa tacgttgaga gggttgtatc
      301 tatggcagac gagggtctta ccaactccgt aaatgaaata agacgcgtgc gtcagtctct
      361 caaggctgcc gctcagcaca aaaaggctcc cgaagagagc aagtaatttt tgctgcctta
      421 tatagtattt tatttcgcct cgttgcatat atttgcttcg aggtgaattt atatgttcgt
      481 atactccgta aagaaaaggc aggtaaaaat ggctctgctt gtgttttttg tggcattgct
      541 tgcagtcgcg ctgattattg tttctcatca tacggcagag gcggggaaaa cggcggcgct
      601 gaatatcaga gcagcttcct ccgaggacag aatcgcgtat ctttcgcagt tcggctggag
      661 cgtaaaagaa gatccggttg aggtttgcga agtgattatt cccgcggaat ttgatgatgt
      721 atacgaaaaa tacaatgagc ttcaaaagga acaaggcttt gatttatcct tgtatgcgtc
      781 gaaacgggta aagcgttgga catatgagat tacaaattat acgggctacg aaaataccgg
      841 ctttatccac gcaaatatct tggtgtatga cggcaaggtg ataggcggag atgtgtgctc
      901 cacagagctt aacggcttta tgcacggctt tgcaaagcct gataacaccg gcgaataaat
      961 aaggagaaac ggatgaaaaa agagcggata gataagctta tagcaggcag cggtaaactc
     1021 agcagaaaag acgtggcagg tctgatttct cacggcaatg ttacgcttaa cggcaggact
     1081 gtcaaaagcg gctccgaaaa ggctgatgcc gatgcggacg agattaaagt atgcggtgaa
     1141 attttcacaa taaaaaaaca tatatacata atgctcaata agccgaaagg cgttgtttcg
     1201 gcaagcgacg gaaagggcga aagaacagtt tgtgaccttg tccccgaaaa gcttatgcgc
     1261 cgtgggctgt tccccgccgg cagactcgat aaagatacta cgggctttgt gctgataacc
     1321 gatgacggcg agttcgcaca ccgtattctc tcaccgaaaa accacattga taaaacgtac
     1381 attgtgtctc ttcgtgacaa tatcgaagaa gaggccgttt cgttgctcga aaacggaatt
     1441 actcttcgtg acgggacggc ttttttgccg tcaaaaataa aaatactctc cgataaagag
     1501 tgcgaaatta ctatttgcga gggtaaatat catcagataa agcgtatgtt ttgcgccgtc
     1561 ggaaacgagg ttacaaatct tcgccgtata aaaatgggac gactcttcct cgatgaaagg
     1621 ctgagagacg gcgagtgccg cgaaataaca gccgatgaat taaaaatgat ttccgaaaaa
     1681 gaataagttg atgcggtatt cgcaaagctc cctttacggg agcttttttg acgtaaaaga
     1741 acaggacgct ctccgaagaa agcgtcctgt tttgcttatt ttagtctatt taatgaattt
     1801 atgactttcc cttactgcca tactgtcgca tcgctcattt tccggatgac ccgcatgccc
     1861 cttgagccaa acaatattaa gactgtgctt tgatatgagc gtcaacagct catcccataa
     1921 atcggaattc aacgccggct tgccgtcgct ctttttccag cccttccttt tccagccctc
     1981 tgcccagcct tttgttatgg cgtccgcaac atattttgaa tccgtataaa g
//
LOCUS       DBMDVO010000206         2029 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26626_length_2029_cov_0.878631, whole genome shotgun sequence.
ACCESSION   DBMDVO010000206 DBMDVO010000000
VERSION     DBMDVO010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26626_length_2029_cov_0.878631"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="rimI"
                     /locus_tag="ACZPE2_03775"
     CDS             <1..282
                     /gene="rimI"
                     /locus_tag="ACZPE2_03775"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR1319001.1"
                     /translation="AGYVGSYFVSGEVYITNVAVKNKYRRKGVAVGLLSELIDLAKEA
                     ESEFVSLEVRCSNTPAISLYGKLGFGKIGIRKNFYENPREDALIMTKYL"
     gene            327..1340
                     /gene="tsaD"
                     /locus_tag="ACZPE2_03780"
     CDS             327..1340
                     /gene="tsaD"
                     /locus_tag="ACZPE2_03780"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1319002.1"
                     /translation="MLILGIESSCDETAAAVVENGRKIVSSVVATQIEKHKIFGGVVP
                     EIASRMHTEAICAVTKQALSDANCKMSDIDAIAVTYAPGLIGALLVGVNYAKGLSLSS
                     GVPLVPVHHLRSHIAANYLSYENLKPPFLCLVVSGGNTLITEVTDYTDFKILGRTRDD
                     AAGEALDKAARTLGLDYPGGVNLDKIAKNGNSDSYKFPRPHVDGSPLDFSFSGLKTSV
                     INLIHNAEQKGETVSVPDVGASFLKAVVDSLEDNVRKALDSTGYDKLVLAGGVSANSV
                     LRERMLSLAKEKNVEIYFPELSLCGDNAAMVGAQGYFEYVSGTRGDMTLNARATLDID
                     SVQ"
     gene            1566..>2029
                     /locus_tag="ACZPE2_03785"
     CDS             1566..>2029
                     /locus_tag="ACZPE2_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR1319003.1"
                     /translation="MAALTTNKSILSWIDEKAALVKPDEIVWIDGSEEQLEKLREEAC
                     KSGEMRKLNEDLLPGCLYHRTAPNDVARVEDRTLICSKKEEDAGPTNHWMEPGKAYEM
                     LYAIAKDSYKGRTMYVIPYSMGPVGSPLAKAGIELTDSIYVVLNMAIMTRVG"
BASE COUNT          602 a          383 c          476 g          568 t
ORIGIN      
        1 gcgggatatg tcggttcata ttttgtctcc ggcgaagtgt atataaccaa tgttgccgta
       61 aaaaacaaat acaggagaaa gggtgtagct gtgggtttgc tctcggagct tatcgatttg
      121 gcaaaagagg ccgaatccga atttgtttct cttgaagtca ggtgttcaaa tacaccggca
      181 atatcgctct atggtaagct gggttttgga aaaataggta tcaggaaaaa cttttacgaa
      241 aatccgcgtg aagatgcgtt gataatgaca aaatatttat aagaaacaac tgaacgtcag
      301 tctttttaat ggaatgtggt ggtttaatgt taattctcgg aatagaaagc tcctgcgacg
      361 aaactgcggc tgccgttgta gaaaacggca gaaaaatagt ttcatctgta gtggcgacac
      421 agattgaaaa acataaaata ttcggcggtg tagtacctga aatagcttca agaatgcaca
      481 cggaagcaat atgtgcggtt acaaaacaag cgttatccga cgcaaattgc aaaatgtccg
      541 atattgatgc catagctgta acctatgcac cggggctgat aggcgcatta cttgtcggtg
      601 taaattacgc aaagggtttg tctctttcat ccggagtacc gttggttccc gtgcatcatc
      661 ttcgttctca tattgccgct aactatttat cttatgaaaa tttgaagcct ccgtttttgt
      721 gccttgtagt ctccggcggg aatacactga taacagaagt tacggattat acggacttta
      781 aaattctcgg cagaacacga gacgatgcgg cgggagaggc actcgataag gctgcgcgta
      841 cattgggact tgattatccg ggcggtgtga atcttgataa aattgcaaag aacgggaatt
      901 ccgactcata taaatttcca agaccgcacg ttgacggttc tccgcttgat ttcagctttt
      961 cggggcttaa gacttcggta ataaatctga ttcataatgc agaacaaaaa ggggagactg
     1021 tcagtgtgcc agatgtcgga gcatcgtttt taaaggccgt tgtggattct ttggaagaca
     1081 acgtcagaaa agcacttgac tctaccggat acgacaagct tgttttagca ggcggcgttt
     1141 ccgctaattc tgttttgaga gaacgtatgc tctctcttgc aaaagaaaaa aacgtcgaaa
     1201 tctattttcc cgagctttca ctgtgtggag acaacgcggc aatggtcggg gcacaagggt
     1261 attttgagta tgtttccggc actcgcggcg atatgacact aaacgccaga gcaacgcttg
     1321 atatcgacag tgttcaataa cttttgccat atcattatgt aatatttgtc atatcatttc
     1381 tatttgttta ttgttcatca aaaatacgat aaatattgac aactatttaa cacttttttg
     1441 cttgccaaaa tgaaagtttt agactataat aattcataat ggcggaggtt gggaaaaccc
     1501 tgaaaattcg tctgaatcag ttaaatattt cggctgtgcc gaattagtta aaaggagata
     1561 atactatggc agcacttaca actaacaaaa gcattttgtc atggattgac gaaaaagcag
     1621 ctcttgttaa gcccgacgaa atcgtttgga ttgacggtag cgaagagcaa cttgaaaagc
     1681 ttcgcgagga agcttgcaag tcaggtgaga tgcgtaaatt aaatgaggat ttacttccgg
     1741 gatgtctgta tcacagaacg gctcccaacg atgttgctcg tgttgaagac agaactctta
     1801 tttgctcaaa gaaagaggaa gatgccggtc ccacaaacca ttggatggag cccggcaaag
     1861 cttatgaaat gctctatgct atcgcaaaag acagctataa gggcagaaca atgtatgtaa
     1921 tcccttattc gatgggtccc gtaggttcac cgctcgctaa ggcaggcatt gagcttaccg
     1981 attcaattta tgttgttctt aatatggcaa ttatgaccag agtcggcaa
//
LOCUS       DBMDVO010000207         2018 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26843_length_2018_cov_1.111633, whole genome shotgun sequence.
ACCESSION   DBMDVO010000207 DBMDVO010000000
VERSION     DBMDVO010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26843_length_2018_cov_1.111633"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="ACZPE2_03790"
     CDS             complement(<1..346)
                     /locus_tag="ACZPE2_03790"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1319004.1"
                     /translation="MKCDKKNMLLYAVTDRAWVGKQTLCEQVEAALKGGCTCVQLREK
                     ELSDAEFLEEAVKIHEICKHYGVPFIVNDNVDIAVKCHAEGIHVGQDDMKASCVREKV
                     GDDMIIGVSAHTV"
     gene            complement(333..1154)
                     /gene="thiM"
                     /locus_tag="ACZPE2_03795"
     CDS             complement(333..1154)
                     /gene="thiM"
                     /locus_tag="ACZPE2_03795"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1319005.1"
                     /translation="MFGEMLQNVRKKRPLIHSITNFVTVNDCANILLACGASPIMADD
                     EREAAEITSACDGLCINIGTLSSRTIPSMLAAGKKANELCHPVVLDPVGVGASHFRTD
                     TAFKLLNNIHFSVIRGNISEIKTLALGTGSAKGVDADIADSFTEETLDKAVAFAKSFS
                     EKTGSVIAITGKTDIVADKDRAFVIRNGNDMMSLVTGTGCQLSSLTAAFIAANQNDIL
                     TAAASAVSAMGLAGETAFGRLSPLDGNASYRNYIIDAACNMTPQGLKEGAKYEMR"
     gene            complement(1228..1743)
                     /gene="thiW"
                     /locus_tag="ACZPE2_03800"
     CDS             complement(1228..1743)
                     /gene="thiW"
                     /locus_tag="ACZPE2_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR1319006.1"
                     /translation="MKKNQVKKLALAGMFCAVAVVGSLFSFPVFGSKCAPIQHMVNIL
                     CAVLLGPWYGLGVAFAASLIRNLLGLGSLMAFPGSMFGALICGLIYKKSKNIPLTLVG
                     EVGGTSLLGGLCAYPVAVLLMGKSASDVAFYAYIIPFFISTAVGSIISGIIVFTLKKC
                     GVLSKMQNGLK"
BASE COUNT          484 a          502 c          469 g          563 t
ORIGIN      
        1 caacggtatg tgcggaaact cctattatca tatcgtcgcc gactttttcg cgaacacagg
       61 aggccttcat atcgtcttgg cctacgtgaa ttccctcggc gtgacatttg acggcaatat
      121 ctacattgtc gttgacgata aacggtacac cgtagtgctt gcatatctca tgtattttaa
      181 ccgcttcctc aagaaattcc gcatcggaaa gctctttttc acggagctga acgcaggtgc
      241 agccgccctt taatgccgct tctacctgtt cgcaaagcgt ctgtttgccg acccaagcac
      301 ggtcggttac ggcgtagaga agcatatttt ttttatcgca tttcatattt tgcaccttct
      361 ttcaatcctt gcggtgtcat attgcaggca gcgtcgatta tgtaatttct gtatgaagcg
      421 ttgccgtcaa gaggggagag cctgccaaat gccgtttcgc ccgcgagccc catggctgag
      481 acggcagaag ccgccgccgt cagtatgtcg ttttgatttg ccgcgataaa tgcggcggtg
      541 agtgatgaca gctggcagcc cgtgcccgtg acaagtgaca tcatatcgtt accgttcctt
      601 attacaaaag ccctgtcctt atcggctacg atgtctgttt ttccggtaat tgcaattacc
      661 gagccggtct tttccgaaaa gctctttgcg aatgctaccg ctttatcgag agtttcctcg
      721 gtaaaactgt cggcaatatc tgcgtcaacg ccttttgcgg agcctgttcc gagtgccaat
      781 gtcttgattt ccgagatgtt gccgcgaatt accgaaaagt gtatgttgtt aagaagctta
      841 aatgccgtgt ctgttctaaa atgcgatgcc ccgacgccga ccgggtctaa aactacggga
      901 tggcaaagct cgttggcttt tttgcctgcc gcaagcattg agggaatcgt gcgagaacta
      961 agcgtgccga tgtttatgca gagcccgtcg cacgcggatg taatttccgc cgcctctctt
     1021 tcgtcatccg ccattatcgg tgacgcaccg catgccaaga gtatatttgc acagtcgtta
     1081 accgtaacga aatttgttat actgtgaatc agcggtcttt tttttctgac gttttgcagc
     1141 atttctccga acattttttc acggcctttc ggttcataat ataagtgcga tttttttgta
     1201 ccgtaaggta cgacttgaca taaagcttta tttcaacccg ttttgcattt tggaaagaac
     1261 gccgcatttt ttaagcgtga aaacaataat tcccgaaata atcgagccga ctgcggttga
     1321 gatgaagaac gggattatat atgcgtaaaa agcaacatcc gaggcacttt tgcccatgag
     1381 taatacggct acgggatatg cacaaaggcc gccgagcaga gaggttccgc cgacctcgcc
     1441 cacgagagta agcggaatat ttttactctt tttataaatt agaccgcata tgagggcacc
     1501 gaacatgctg ccggggaaag ccataaggct gccgagcccg agcagatttc tgatgagaga
     1561 tgccgcaaag gcgacgccga gaccgtacca cggaccgaga agtacggcgc agagaatgtt
     1621 taccatatgc tgtatcggcg cacacttact gccgaatacc gggaaagaga ataaactgcc
     1681 gacaacggct accgcacaaa acatacctgc aagggcaagc tttttaacct gatttttttt
     1741 cattttaatt gctccttaga aagttttata tcggtcgagg ctttttggcc tcctctcacg
     1801 ccggaacacg gcttcccatc gcagcagtgt tgtcatcata cggtgcaaca ttgaaattac
     1861 aaaactcagg gcgctccccc tgctttttgc cgcgtcgttt tccccgcggg gcattttcac
     1921 atcctttttt gtgcacagtc cgacttttat ttttgacaaa ataaaaaacg gagcttttca
     1981 ctcaaaatgc gaagctccgt ttaacaagtc tttttcgg
//
LOCUS       DBMDVO010000208         2015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26896_length_2015_cov_1.252351, whole genome shotgun sequence.
ACCESSION   DBMDVO010000208 DBMDVO010000000
VERSION     DBMDVO010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26896_length_2015_cov_1.252351"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="ACZPE2_03805"
     CDS             complement(<1..508)
                     /locus_tag="ACZPE2_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1319007.1"
                     /translation="MKKRISHSVFFVSLAVNICCATLSLAVLYNRFKADVGSAGAEEL
                     PSDFLLAVLVSAVISFAAALFTARVVSKRTLKELNDINLDDPNDSNFGSELSLLFYKI
                     RSQNSQIQSQLRELERSQQEFKMITENMSEGLIIIDLKHSILSFNRSAKQIFGVENIK
                     KHENVFTIN"
     gene            complement(514..1191)
                     /locus_tag="ACZPE2_03810"
     CDS             complement(514..1191)
                     /locus_tag="ACZPE2_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1319008.1"
                     /translation="MIYLVEDDKSIRELLVYTLNNSGLPANGFEKPSEFYRELRADDA
                     DLIILDIMLPECDGLTMLKNLRADEKTKHIPVMMLTAKGSEYDKVVGLDMGADDYMTK
                     PFGMMELIARVKALLRRTKESGKNDNVNTLNIGCLSVIPSKYSVKVGGEKVVLTLKEF
                     DLLCLLLQNKGTVFTRDKLLNKIWGYDFDGESRTVDVHVRTLRQKLGEAGKYIETVRG
                     IGYKIGD"
     gene            1591..1947
                     /locus_tag="ACZPE2_03815"
     CDS             1591..1947
                     /locus_tag="ACZPE2_03815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1319009.1"
                     /translation="MNIKRGDIYYADLSPVVGSEQGGIRPVLIVQNDVGNKYSPTVIA
                     AAITSQKYKTKLPTHISVDAVDCGLQKDSIVLLEQVRTLDKKRLKERMGNLPTDEMNK
                     VNRALSVSFGLGQTMR"
BASE COUNT          566 a          462 c          418 g          569 t
ORIGIN      
        1 gatttattgt aaaaacattt tcatgctttt taatattttc aacaccgaat atctgctttg
       61 cgctgcggtt aaacgaaagt atgctgtgct taaggtcgat tattataaga ccctcagaca
      121 tattttcggt tatcatttta aattcctgct gactgcgttc aagctctcga agctgcgact
      181 gaatctgcga attctgactg cggattttat agaacagcaa agacagctcc gagccgaaat
      241 tactgtcgtt tgggtcgtcc aaatttatgt cgttgagttc ttttaaggtt cgctttgaca
      301 ctactcgcgc cgtaaaaagc gcggcggcaa aggatattac cgcggaaaca agcactgcca
      361 aaagaaaatc cgagggcagc tcctccgcgc cggcggagcc gacatcagcc ttaaagcgat
      421 tataaagaac ggcaagcgac agcgtagcac agcaaatatt taccgcaagc gacacaaaaa
      481 acacggagtg agaaattcgt tttttcattc ggttcagtcc cctattttat agccaatacc
      541 tcggacggtc tctatgtatt tccccgcttc gccgagtttt tggcgaaggg ttctgacgtg
      601 cacatccacg gttctgctct cgccgtcgaa gtcatatccc caaattttat ttagaagctt
      661 atcccgcgtg aaaacagtac ccttattttg caaaagcaaa cacaaaaggt caaattcctt
      721 cagcgtcagc accacctttt cgccgcctac tttgacggaa tacttagatg gaataacact
      781 gaggcagccg atattaaggg tattgacgtt gtcattctta cccgactcct ttgtgcggcg
      841 caaaagagct ttgactctcg ctatcagctc catcattccg aaaggctttg tcatataatc
      901 atccgcgccc atatcaagcc ccacgacctt atcgtattcc gagcccttgg cggtcagcat
      961 catcaccggg atgtgcttcg ttttttcgtc ggctctcaaa tttttaagca ttgtaagccc
     1021 gtcacattcg ggaagcatta tatcaaggat aatgagatct gcatcatctg ccctaagctc
     1081 tctgtaaaat tcactcggtt tttcaaatcc gtttgccgga agtcccgaat tattaagagt
     1141 gtatacaaga agctctctta tactcttatc gtcctcaaca agataaatca tatttttctc
     1201 ctatcggcag attttatctt tgcagccttt ttcgattctt agtataatct gtttttacat
     1261 ttgttgcaag actgccgcct tatatttcat tttatccgcg aacggtaaaa tttgttgtat
     1321 tgcatatgta aaatctctgt aaagttatat tccgcgtttt gcggcacatt ttgccgtcat
     1381 atgccatatt atgtgctgag ggaaaatctc cgcctttcaa atatgtgcct taaattcggt
     1441 ttatatgttt atagtttcct tttatgggca aaattatatt gagcaagata gcaaaaccca
     1501 ttatgccgaa aacaggtcgg cgcggcagct cagagccgta ttgaattatg cttcgcttgc
     1561 ttaagggaat taaaacagga gagtgaggaa atgaatatta agcgcggcga tatttattac
     1621 gccgatttaa gccccgtggt aggctcggag caaggcggaa tacggccggt acttatagtt
     1681 caaaacgacg taggcaacaa atacagcccc acagtaattg cggcggcaat aacaagccaa
     1741 aaatacaaaa caaagcttcc gacacacata agcgtcgatg cggtagactg cggactgcaa
     1801 aaggattcca tcgttttgct tgagcaagtc agaacgctcg acaaaaaacg tcttaaagag
     1861 cgcatgggaa acttgcctac ggatgaaatg aacaaggtaa acagggctct ttccgtaagc
     1921 ttcgggctcg gacaaacgat gcgttaagca agaaaaaagt gattctccgc agaaatgcag
     1981 ggaatcactt agtcttaact gcttgctata ccgaa
//
LOCUS       DBMDVO010000209         2012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26952_length_2012_cov_1.035060, whole genome shotgun sequence.
ACCESSION   DBMDVO010000209 DBMDVO010000000
VERSION     DBMDVO010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26952_length_2012_cov_1.035060"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            111..1481
                     /locus_tag="ACZPE2_03820"
     CDS             111..1481
                     /locus_tag="ACZPE2_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319010.1"
                     /translation="MTKQNSMLTGKPIKSIIKFSLPIMASSLLQYNYTLVDNILVGRY
                     VSTDALAAVGGVASINSFIVGAALGLTSGFTIPVAHAYGAKDQDRVNRYSGSSITVSF
                     LLGLLLVVVAHIISSPLLKLIGTPDEILGLSSAYVNILYFAIPFQMLSNNFTAISRAV
                     GESRKPLIYFTASIFVNFVLDMLFIKVFRWSVEGAALATLISHITAAVLTGNYILRRN
                     TSVHISKKEFKLDLKTAFIQLKLGIPVSLQFTVTSIGSMCLQSAVNSFGTATIAGFTA
                     AGRVENIANIPLSGLSVGTQTFVGQNYGAGKYDRIIKSVKKIFTLNILLSVALSVALL
                     TAGMPIVRLFMTEPNEDILFAAHKYLIATAECFSLVAVLFVLRNTLQGLGFTYANSVA
                     GAGELIGRISIALIFTPLIGFNAVCYAAPAAWLLADIPLAIIYLKKKKKFKALAMRNM
                     NTTADI"
BASE COUNT          578 a          425 c          409 g          600 t
ORIGIN      
        1 aaagctctcc cggcagaata tttttataga cataatactg cacaagtgct aaaatttaag
       61 caaattctaa ttgaatttaa tttttaaaaa tactttggaa gagttggcaa atgacaaaac
      121 aaaattctat gcttaccggc aagccgataa aaagtataat taaattctcg ctgccgatta
      181 tggcttcatc gcttttacag tataactata cattggtcga caatatactt gtcgggcgat
      241 atgtaagcac ggacgctctt gccgcggtcg gcggagtcgc ctcgataaac tcgtttattg
      301 tcggtgcggc actcggcctt acaagcggat ttacaatacc ggtggcgcat gcttacggcg
      361 caaaggacca agacagggtc aacagatatt ccggaagcag cattacggtg tcgttcttgc
      421 tcggcctatt acttgttgtt gtcgcgcaca ttatttcctc tcctcttttg aagcttatcg
      481 gcactccgga cgaaattctc ggtctctcct cggcgtatgt aaacattctt tactttgcaa
      541 taccgtttca gatgctctca aacaatttta ccgcaatttc aagagccgtc ggtgaaagca
      601 gaaaaccgct catatatttt acggcaagta tttttgtaaa tttcgtgctt gacatgctgt
      661 tcataaaggt atttcgctgg agcgttgagg gagctgcgct tgctacgctt atttctcaca
      721 ttacggcagc cgttcttacg ggaaattaca ttttaaggcg caatacaagc gtacacattt
      781 caaaaaaaga gttcaaactc gacctcaaaa cagcattcat acagttaaaa ttgggaatac
      841 ccgtttcgct tcagtttacc gttacctcta tcggatcgat gtgtcttcaa agcgcggtaa
      901 attcattcgg cacggcgact attgcaggct ttaccgccgc aggaagggtc gagaatattg
      961 caaatatacc gctgagcgga ctcagcgtcg gtacacaaac cttcgtcggg caaaactacg
     1021 gggccggaaa gtacgatcga ataattaaat cggttaaaaa aatatttacg ctcaacatac
     1081 ttctttccgt cgctttaagc gttgctcttc tgacggcagg tatgccgata gtcaggctgt
     1141 ttatgacaga gcctaatgaa gacatcttgt ttgccgccca taaatatctt atcgccacgg
     1201 cggagtgctt cagtcttgtc gctgtgctgt tcgttttaag gaacactctg cagggtctcg
     1261 gctttacata tgcaaattcg gttgcgggtg ccggcgagct gataggaaga atatcaatag
     1321 cacttatttt cactccgctt atcggcttca atgcagtatg ctacgcagcc cctgccgcat
     1381 ggcttcttgc agacatcccg cttgcgatta tatatttaaa aaagaagaag aaatttaagg
     1441 cgttagcaat gcggaatatg aatacaacag cggatatata gcccttgaaa tttctttatc
     1501 cgcaaacgaa ataaaaagaa ttatcattaa acagtcgagt gaatctcgtt ttatatatcg
     1561 attttataaa attatttata ttgaagtcag ccgagaaagg aagcaaggct tttcggcaat
     1621 ggatttatat gcaaaaaacc aacacttcct tatgaacact cagccaacgg agcgagattt
     1681 tttgttaaac accgaatttt aaaattcgag acgtaaaaat cgataatcgg tcgcgacatt
     1741 tacgtttgct tttttgtata ccaaaacagg gtcccttgac aaaaaacaaa gtccaatgct
     1801 taatattttt tgcagcacaa gctcactttt gccctgttgc gtattaaccg agcgataatt
     1861 atttaaaatg aggtgccttt atgaaaaaat ataatataac gacattgatt ttaatgatat
     1921 tggtttgtct cggaccgccg ctgaatcttt tatttgagta ttttgtttta catcaaaatc
     1981 cgtctcctgt tttgtggggt acaacgattt tc
//
LOCUS       DBMDVO010000210         2011 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_26969_length_2011_cov_1.334031, whole genome shotgun sequence.
ACCESSION   DBMDVO010000210 DBMDVO010000000
VERSION     DBMDVO010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26969_length_2011_cov_1.334031"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="ACZPE2_03825"
     CDS             complement(<1..272)
                     /locus_tag="ACZPE2_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014997.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HIR1319011.1"
                     /translation="MAYLDKVKLADYTRKEDWLNSISHMVGGGLSVVALLMCCIKSII
                     LRRWDYLLLSFIYGITMIAMYSCSSVYHALRPNNGKKVMRMIDHSM"
     gene            complement(407..895)
                     /locus_tag="ACZPE2_03830"
     CDS             complement(407..895)
                     /locus_tag="ACZPE2_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR1319012.1"
                     /translation="MAVCIIYASKYGSAKKVAGLIAEKLGGCDIFNIAEDSFDLSKYN
                     FVIIGSDIRMGTVDKLITKLLFRFIKPLSERKCAYFLTCIFPENGDGYFKRNIPSQLL
                     SESVAVAAIGGELDFKRLRGADRFAANMILKAEREKDVYKTFSLDTDKISDFVDKLKP
                     QL"
     gene            1426..1575
                     /gene="rpmG"
                     /locus_tag="ACZPE2_03835"
     CDS             1426..1575
                     /gene="rpmG"
                     /locus_tag="ACZPE2_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1319013.1"
                     /translation="MRVKITLACTECKQRNYNTMKNKKNSPDRLEMNKYCPFCKKHTL
                     HRETK"
     gene            1590..1976
                     /gene="secE"
                     /locus_tag="ACZPE2_03840"
     CDS             1590..1976
                     /gene="secE"
                     /locus_tag="ACZPE2_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1319014.1"
                     /translation="MADENKKVSAENTKPAKQVKDKKAEKPAKTAKVKDKKPNVFVRM
                     GRSIKKFFKELRGESKKVVWPDRKTVLKSSLVVIVSVVIIGVVIWLIDTGLSELVKLL
                     INLASKSGGGAEAATAGAITANFFSL"
BASE COUNT          602 a          432 c          424 g          553 t
ORIGIN      
        1 atcatcgagt gatctatcat tctcattacc tttttgccgt tgttagggcg caatgcgtgg
       61 taaaccgaag agcacgaata catagctatc atggtgatac cgtatataaa gctcaaaagc
      121 agataatccc atctgcgaag aattattgat tttatgcagc acatcaaaag tgccactacc
      181 gacagtccgc cgcctaccat gtgtgatatt gaattgagcc agtcttcttt tcttgtgtaa
      241 tcggcaagct tgaccttatc gagatatgcc atattttttc ctcctttagc tctcgttcgt
      301 ttgtgtctcc attttagctt atgaattatt aacttcaaga cattctgctt tgcaaaacga
      361 aaaaagcgga aaatataggt tgcggagcgg tagcgaaagc cgcaacttaa agttgcggct
      421 tcagtttatc aacaaaatcc gatattttgt cggtgtcgag cgaaaacgtc ttgtaaacat
      481 ccttttcacg ttcggccttt aaaatcatat ttgcggcaaa tcggtctgct ccgcgcagcc
      541 ttttgaagtc aagctcgccg cctattgcgg cgacggcaac ggattcactt aaaagctgag
      601 aggggatatt gcgcttaaaa taaccgtcgc cgttttcggg aaaaatgcag gttaaaaaat
      661 atgcgcattt tctctcggaa agcggcttta tgaaccgaaa gagcagcttt gttatcagct
      721 tgtcaaccgt tcccatgcgt atgtcgctgc cgattatgac aaaattatat ttagacaggt
      781 caaagctgtc ctcggcgata ttgaaaatat cgcatccgcc gagcttttcg gcaatcaacc
      841 cggcaacctt ttttgcggag ccgtatttgc tcgcatatat aatgcataca gccattttct
      901 acctcttaaa cagaatgtaa aatcattatt tcagttcgga agaaattttg tccttttcct
      961 taacggcatc atacatacag aaaagagtgg tgacggcgga aaaaatcaaa gccgaccatt
     1021 tgaacatctt ttttatgaat tccatttgat atttgcgcct ttccgactgt ccgccgcggc
     1081 ggcagtctgt cattgcttgt tgtcacgtct tgattttttg ctttttatat actattatat
     1141 aatatatccg gcgtatataa aaagtcaaac atattttttc gtttccatga atcgcgagga
     1201 gagaagcttc tttcgcagga cggacggaac aattaacaaa aaccgcaaaa ttcacgactt
     1261 gccggacact gtcgccgcaa attgaataaa aactattgac ttaaagccca cagtgtgata
     1321 tagtaactgt acctctgaaa gagggttctt tttttgtgcc cgcttttttc gaggtaggct
     1381 aacataagcg gccgtgagcc gccaataacg gaggtgccga aataaatgag agtcaaaatc
     1441 acattagctt gcactgaatg caaacagcgc aactacaaca cgatgaagaa caagaaaaat
     1501 tcacccgaca ggttggagat gaataagtat tgccccttct gcaagaagca tactctccac
     1561 agggaaacta agtaagcgga ggtaaaaata tggctgacga aaacaaaaaa gtttctgccg
     1621 aaaataccaa acccgcaaag caagtgaaag acaaaaaggc tgaaaagcct gcaaaaaccg
     1681 caaaagtaaa ggacaagaag cctaacgtat tcgtacgtat gggcaggagt ataaagaagt
     1741 tctttaagga acttcgcggc gagtccaaaa aggtcgtttg gcccgacaga aaaaccgtac
     1801 tcaagtcctc gcttgtagtt atagtttcgg ttgttattat cggagtcgtt atatggctta
     1861 ttgacaccgg actttccgaa ctcgttaagc ttcttataaa tctcgcttcc aaatcaggcg
     1921 gcggagcaga ggctgcgacg gcaggtgcta ttacagcaaa ctttttcagc ttataacgga
     1981 ggtagcatat ggcagatatc tccaaatggt a
//
LOCUS       DBMDVO010000211         2010 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27001_length_2010_cov_1.152436, whole genome shotgun sequence.
ACCESSION   DBMDVO010000211 DBMDVO010000000
VERSION     DBMDVO010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27001_length_2010_cov_1.152436"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="ACZPE2_03845"
     CDS             complement(<1..1584)
                     /locus_tag="ACZPE2_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319015.1"
                     /translation="MQKNSKIKIGKSITAVLLCVVLLVTSIPMVLAAGEGEYDPIPTF
                     TESDKREAAATLNDDGGITVSFPAAKVSERKTGTKTIDGYMLELVDLGTYTEVHTSTV
                     LLRKMVSPTGTAPYTAEITADEIKTKVPAGLSDDHRYNITITAYDSDGWFSEAVNAYV
                     SDVPTYVYDADAYAPVTNNVLAMREVLTFDKKGQADSHGSFSDDGTDERTAEINNVRS
                     TSGSLDYMQEGNHLTVGGRTDGVGVKDTGNSYGYAMRIESAATTDSPQSFDTTWSRQT
                     WDPTGASEIWYYLDFSQVSVTGLSFRLRSNEKYFAQDFDNSYNDKDNAFNRYGDMIYS
                     TVGTTKAGYMGEDPYLYLQQTDGSWKKVMLKDGTVDLDHFKGYVRIPLQFMCSETDSV
                     VTESNEDFGVNKPVSDMYVWNNSDKTKANADKYYNEKIKLSQSVVVDPAGTSISDALL
                     IRRYSFRINKTSTLSGEYYIDGGTMLAPCMTTADGGKTTADSPKRAYIDKTKICTGED
                     PVVNRTNSFKAIEDIMSAGF"
     gene            complement(1631..>2010)
                     /locus_tag="ACZPE2_03850"
     CDS             complement(1631..>2010)
                     /locus_tag="ACZPE2_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319016.1"
                     /translation="IDQNAILGEGEISVPDKTKTRGRQIKVYGIPELPLAANTKEQSF
                     VFSNPEENPCFFVIEIELSDTGEVIYTSNLLPPGYSISKFTLNRELAAGTYQATIHVK
                     TYSFDKEQRKLNNMDLKTTIVVS"
BASE COUNT          482 a          484 c          458 g          586 t
ORIGIN      
        1 aaagcctgcg gacataatat cctcgatagc tttaaaagaa tttgtacgat taactaccgg
       61 atcttcaccc gtacagattt tggtcttatc gatataagct cttttgggcg aatcggctgt
      121 tgtcttgcca ccgtccgccg tagtcatgca cggagcgagc atcgtgccgc cgtcgatata
      181 gtattcaccg ctcaaagtcg acgtcttgtt gattctgaag gaatagcgtc taataagcag
      241 agcatcggat atcgaagtac cggcagggtc aacaactacc gactgcgaaa gcttaatttt
      301 ctcgttataa tacttgtcgg catttgcttt agttttatcc gagttgttcc acacatacat
      361 atcagacaca ggcttgttta caccgaaatc ctcgttggat tccgtgacaa cggagtcggt
      421 ttcggagcac ataaactgaa gcggtatgcg gacataaccc ttgaagtgat caaggtcaac
      481 agtaccgtct tttaacataa cctttttcca gctaccgtcc gtttgctgca ggtataaata
      541 tggatcctca cccatatagc ctgcttttgt ggtaccgaca gtagaataaa tcatatcgcc
      601 gtatctgttg aaagcgttat ccttgtcgtt atagctatta tcaaagtcct gtgcaaaata
      661 cttttcgttt gaacgaaggc ggaatgaaag ccccgttacg ctgacctgtg agaagtcaag
      721 atagtaccaa atctcggaag cacccgtcgg gtcccaagtc tggcggctcc atgttgtatc
      781 aaaggactga gggctgtcgg tggttgcggc ggactcgata cgcattgcgt agccgtagct
      841 gttgcccgtg tctttaacgc ctacaccatc ggttctgcct ccgactgtaa ggtgattacc
      901 ctcctgcata tagtcaagtg agcctgacgt actgcgtaca ttgttgattt cggcggttct
      961 ctcgtcggtt ccgtcatccg aaaacgaacc gtggctatct gcttgacctt ttttatcaaa
     1021 ggtaaggact tcacgcatgg caagaacgtt gtttgtaacg ggggcataag cgtccgcatc
     1081 gtatacataa gtcggaacat ccgaaacgta tgcgttaaca gcctccgaga accagccgtc
     1141 gctgtcgtac gccgttatcg taatattgta acggtgatcg tcagacagac ccgcaggcac
     1201 ctttgttttg atttcgtctg ccgtaatctc agccgtgtac ggtgcggtac cggtgggact
     1261 aaccatcttg cgcagcaaaa ccgtgcttgt gtgaacctcg gtatatgttc cgagatcaac
     1321 aagctctaac atatacccgt caattgtttt tgtgccggtt ttgcgctcgc taaccttagc
     1381 cgcggggaag ctgaccgtaa ttccgccgtc gtcattaagt gttgcagccg cttccctttt
     1441 atccgattcg gtaaatgtcg gtatcgggtc atattcgccc tcgccggccg cgagaaccat
     1501 gggaatcgac gttaccaaaa gcactacgca aagcagcaca gcagttatgc tttttccgat
     1561 tttaatcttt gaattttttt gcataaactg caactccttt cctcaaaatc tgtattttga
     1621 atatagtttc ttatgaaacc acgatggtcg tttttaaatc catattgtta agtttacgct
     1681 gctctttatc aaagctgtat gtcttaacat ggattgttgc ctgataggtt cctgccgcaa
     1741 gctcacggtt cagcgtgaac tttgatattg aatagccagg cggcaagaga tttgaggtat
     1801 atataacctc gcctgtatct gaaagctcta tttctataac aaagaagcac gggttttcct
     1861 caggatttga gaatacgaag ctctgctcct tggtatttgc cgccaaggga agctcgggaa
     1921 taccgtagac ctttatctgc ctgccccttg tttttgtttt atcgggtacg gaaatctcgc
     1981 cctcgcccaa tatggcattt tggtcgatta
//
LOCUS       DBMDVO010000212         2008 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27043_length_2008_cov_0.837441, whole genome shotgun sequence.
ACCESSION   DBMDVO010000212 DBMDVO010000000
VERSION     DBMDVO010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27043_length_2008_cov_0.837441"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            207..283
                     /locus_tag="ACZPE2_03855"
     tRNA            207..283
                     /locus_tag="ACZPE2_03855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:241..243,aa:Pro,seq:cgg)
     gene            408..>1462
                     /locus_tag="ACZPE2_03860"
     CDS             408..>1462
                     /locus_tag="ACZPE2_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1319017.1"
                     /translation="MKRLLKNGTVVNVFTGELIKTNVLFENGIILGVDDYGDDEADEV
                     IDATGKYICPGFIDGHIHIESTMLTCPEFAKTAVSHGTTAVIADPHEIANVSGKDGLR
                     YMLESSEGLPLTVYFVIPSCVPATGFDESGAVLSADDIEEFYSESRVLGLGEVMNYVG
                     VVAGDGELEKKIKSALSRGLVVNGHAPLLSGKPLDKYITAGIGDDHECSSADEAKERI
                     RKGQRIMIRQGTAARNLSGLISLFDDPWEQRCLLVTDDKHPADLISNGHIDSIIRSAV
                     QMGKSAVTGIRMATLHAAECFGLKNTGAVAPGYKADLLVLDDLDKVIVRDVYKNGEKV
                     VSDGKTLCFDMPKINDAL"
     assembly_gap    1463..1472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1473..>2008
                     /locus_tag="ACZPE2_03865"
     CDS             <1473..>2008
                     /locus_tag="ACZPE2_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1319018.1"
                     /translation="EKKVRSSFNLPILSSSDFDVDYNGKRKCHVISLVDGQLLTDDLV
                     SDIDFDCNNGIDIEHDILKIAVIERHHNTGHIGNAFIKGIGLKEGAIASSVAHDSHNL
                     IVIGTNTDDMAFAANRIRELGGGIVSVKNGKVVAEMPLPIGGLMSCETAERAAEENEK
                     VRESVYSLGVPKGFEPFM"
BASE COUNT          556 a          395 c          509 g          538 t
ORIGIN      
        1 aggcgaactt aaagccttac gggcttgtgt aatttgattg cgatttgagc cctgcctgca
       61 aaaatttagg cttgctttag tatatctcgg catcatcctt aaaactcgtt tttcctccga
      121 tttgcctaaa aattgaaaac atttaataaa aaatcaaaat accacttgat tttatcgctc
      181 cgatataata taatactaaa gcacttcggg gtgtggcgaa gtttggtatc gcgcttggtt
      241 cgggaccaag aggccatggg ttcaagtccc gtcactccga ccaaaaaaag gtaagtatcg
      301 taagatgctt accttttttt atttatccgc aactttttgt agctatgaaa ttgtttttgt
      361 gatacaatca tttacagaat atactttgac ttgttaagga gcgcgctatg aaaagattat
      421 taaaaaacgg aacggtagtc aatgtcttta cgggtgagct cataaagacg aacgttcttt
      481 ttgaaaacgg aattattttg ggtgtggacg actacggtga cgatgaagca gatgaagtaa
      541 tcgacgctac cggaaaatat atttgcccgg gctttataga cgggcatata cacatcgaaa
      601 gcacaatgct cacttgcccg gaatttgcca aaacggcggt ttctcacggt acgaccgcgg
      661 taatagccga tccgcatgaa attgccaatg tcagcggtaa agacggtctt cgctatatgc
      721 ttgaatcaag cgagggactt ccgcttaccg tgtattttgt aataccgtca tgtgttccgg
      781 cgacaggctt tgacgaatcg ggcgcggttc tctcggcgga tgatatagag gaattctatt
      841 ctgaaagtcg tgttctcggg ctcggcgagg ttatgaatta tgtcggcgtg gttgccgggg
      901 acggcgagct tgagaaaaaa atcaaatccg cattgtctcg cggacttgtt gtaaacggtc
      961 atgctccgct tttgtcgggc aagccgcttg ataaatacat taccgcggga ataggagatg
     1021 accatgaatg ctcttcggcc gatgaagcca aggagcgcat acgcaagggg cagagaataa
     1081 tgataaggca gggaactgcg gcgagaaatc tttcggggct gatttcgctg tttgacgacc
     1141 cgtgggagca gagatgcctt ttggtgaccg atgataaaca cccggctgac cttatctcaa
     1201 acggtcatat agacagtata atccgctctg ccgtgcaaat gggcaaaagc gccgtcacgg
     1261 gtataagaat ggcgaccctc catgctgcgg agtgcttcgg acttaaaaat acaggcgcag
     1321 tagcacccgg ttacaaagcg gatttgcttg tccttgatga ccttgataag gtaatcgtgc
     1381 gcgatgtata caaaaacgga gaaaaggttg tttcggacgg caaaacgctt tgttttgata
     1441 tgccgaaaat aaatgatgct ctnnnnnnnn nngaaaaaaa agtgcgtagc tcgtttaatt
     1501 tgcccatact gtcttcgtct gattttgatg tcgattataa cggtaaaaga aaatgccatg
     1561 ttatttccct tgtggacggg cagcttttga ccgatgattt ggtatcggat attgatttcg
     1621 actgcaataa cggcatagat atcgagcatg atatattgaa aatagccgtt attgaaagac
     1681 atcacaacac gggtcatata ggaaatgctt ttataaaggg tatcggactt aaggaaggtg
     1741 ccatagcatc ttccgtggcg catgattcgc acaatcttat tgtgataggc acaaataccg
     1801 atgatatggc gtttgccgca aaccgtatac gtgagctcgg cggcggtata gtttcggtta
     1861 aaaacggaaa agtcgtggcc gaaatgccct tgccgatagg cggacttatg tcatgtgaaa
     1921 cagcggaaag agcggcggaa gaaaatgaaa aggtgcgcga aagcgtgtat tctctcggtg
     1981 tgccgaaggg ctttgagccg tttatgat
//
LOCUS       DBMDVO010000213         2003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27122_length_2003_cov_1.382229, whole genome shotgun sequence.
ACCESSION   DBMDVO010000213 DBMDVO010000000
VERSION     DBMDVO010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27122_length_2003_cov_1.382229"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="ACZPE2_03870"
     CDS             <1..1523
                     /locus_tag="ACZPE2_03870"
                     /inference="COORDINATES: protein motif:HMM:NF046196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="HIR1319019.1"
                     /translation="VNSAIEEFLKKGYDEVKKEAIAEYKSLFDRFSFEVTSFTSTEYT
                     DKLLSSYRENPDSKDGRYLEELLCQYGRYLLISSSAENDELPANLQGVWNNSNSPAWS
                     CDYHLNVNLQMCYWHAYVTGLFGAAKPMIRYMESLREPGRVTARCYHNIVSDEKNPEN
                     GWVCHTQNTPFGWTCPGWDFYWGWSPAASSWMMQNCYDYYAFTEDTDYLRSDIYPMMK
                     ENAVFWLQNLVYSKEQDRYVSSPSYSPEHGPISIGNTYEQTLIWQLFTDTEKAARVLG
                     DDDFAAELEKVRVKLKPITKGRWGQIKEWYEEDEWYKSLKFRKLKYKLHSCQNRHRHA
                     SHLLGLYPGNAITDKTPELIEACKVSLLDRGFGQKSGANGSGWGKANKVNLWARAKDG
                     NRAYSMLRELINKNIAPNLWDFHPPYQMDGNCGYTSGVCEMLCYSSDDIIELLPALPE
                     EWKNGSVKGLCARGGYSLDFTWENGEIKSLSVYSSCGKKTARIKTGDEIKTVKTVG"
     gene            1571..>2003
                     /locus_tag="ACZPE2_03875"
     CDS             1571..>2003
                     /locus_tag="ACZPE2_03875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1319020.1"
                     /translation="MKYLVIGVGGTGGAIAAYLTKAGIDTTLIARGKNLSALRENGIT
                     LSLDNNEYTNAVKAFDMNGFENAVKSGAALSPDVIFVCVKGYSITDTIPFIKSVSKPE
                     TVVIPILNIYGTGRHMQKELPSLTVTDGCIYVSSSLVSPGKI"
BASE COUNT          577 a          431 c          504 g          491 t
ORIGIN      
        1 aagtaaattc cgcaatagaa gaatttttga aaaaaggcta tgacgaggtt aaaaaagagg
       61 cgattgccga atacaaatcg ctgtttgacc gtttttcatt tgaggttaca agcttcacaa
      121 gcaccgaata cacggataaa ctcctttctt cataccgtga aaaccccgat tcaaaggacg
      181 ggcggtactt ggaagagctt ttgtgccaat acggcagata cctgcttatt tcaagctctg
      241 ccgaaaacga tgagctgccc gccaatcttc agggcgtatg gaacaattcc aattcgcctg
      301 cgtggagctg tgattatcat ctgaacgtaa atcttcagat gtgctattgg catgcgtatg
      361 tcacgggact tttcggtgcg gcaaaaccga tgatacgcta tatggagtcg cttcgtgagc
      421 cgggcagagt taccgcgcgg tgttaccaca atatcgtttc cgatgaaaaa aatcccgaga
      481 acggctgggt gtgtcatact caaaatacgc ccttcggttg gacttgtccc ggttgggatt
      541 tctattgggg ctggtctccg gcggcatcct cgtggatgat gcagaattgc tatgattatt
      601 atgcgtttac cgaggatacg gactatcttc gctccgacat atatcccatg atgaaagaaa
      661 atgctgtttt ttggcttcaa aatcttgttt acagtaaaga gcaggacaga tatgtttcgt
      721 ctccgtctta ttctcctgag cacgggccca taagtatagg caatacatac gagcagacgc
      781 ttatatggca gctgtttacc gataccgaaa aggcggcaag ggttctcgga gacgacgact
      841 ttgcggctga gcttgaaaag gtacgtgtaa agttaaagcc cattacaaag ggacgctggg
      901 gtcagataaa ggaatggtat gaggaggacg aatggtataa gtccttgaag ttcagaaagc
      961 ttaaatataa gctccattcc tgccaaaacc gccacagaca cgcatctcat ttgctcggac
     1021 tttatcccgg aaacgcgata accgataaaa ctcccgaact tatcgaggct tgtaaagtat
     1081 ctctacttga cagaggcttc ggccaaaaga gcggcgcaaa cggctcgggc tggggcaagg
     1141 cgaataaggt aaacctttgg gcgagggcaa aggacggcaa cagagcatat tcaatgctgc
     1201 gtgagcttat aaacaaaaac atcgcgccga acctttggga tttccatccg ccgtatcaga
     1261 tggacggaaa ctgcggatac acaagcggtg tctgcgaaat gctgtgttac agctccgatg
     1321 atattataga gcttttgccg gcactccccg aggagtggaa aaacggcagc gtaaaagggc
     1381 tttgtgcccg cggaggatat tctcttgatt tcacgtggga aaacggcgaa ataaagagtc
     1441 tgtcggtata ttcgtcctgc ggcaaaaaga cggcacgcat taaaacgggc gatgaaatca
     1501 aaacggtgaa aacggtcggt taaaagcacc gaaaatttac tcgttgtatt tttgaaagga
     1561 tttgaaaaca gtgaaatatc ttgttatagg tgtcggcgga acgggaggcg caatagccgc
     1621 gtaccttaca aaggccggaa tagatacaac tctgattgcg agaggcaaaa atctgtcggc
     1681 tctcagggaa aacggcatta ctctgagcct tgataacaac gaatacacaa atgccgtaaa
     1741 agcctttgat atgaacggtt ttgagaatgc tgtgaaatcc ggcgcagctc tttctcccga
     1801 tgttattttt gtgtgcgtaa aggggtatag cattacagac acaataccgt ttataaaaag
     1861 cgtatccaag cccgaaaccg tggtaatacc gatactcaat atttacggca cgggcagaca
     1921 tatgcaaaaa gagcttccct ctcttacggt gacggacggg tgtatatatg tttcctcaag
     1981 tcttgtctct ccggggaaaa tac
//
LOCUS       DBMDVO010000214         2002 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27144_length_2002_cov_1.169911, whole genome shotgun sequence.
ACCESSION   DBMDVO010000214 DBMDVO010000000
VERSION     DBMDVO010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27144_length_2002_cov_1.169911"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="ACZPE2_03880"
     CDS             complement(<1..526)
                     /locus_tag="ACZPE2_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1319021.1"
                     /translation="MSEGCTHDCSTCGENCSERKAEDMKLRQNDYSNIKKVIAVVSGK
                     GGVGKSLVTSLLASASQARGFETAVMDADVTGPSIPKAFGIHERAMGSDEGMFPVESK
                     TGIKIMSINLLLEKEDAPVVWRGPVISGVINQFWQEVLWGDVDYMFVDMPPGTGDVPL
                     TVFQSLPVDGIIIVT"
     gene            complement(523..990)
                     /locus_tag="ACZPE2_03885"
     CDS             complement(523..990)
                     /locus_tag="ACZPE2_03885"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR1319022.1"
                     /translation="MDKNDFMRLALLEAEKAFLDGEVPVGAVIVRDGEVVSTGRNRRE
                     KEKNALCHAEIEAIDGACRALSGWRLPRCEMYVTLEPCPMCAGAIINSRIEKVTFGAY
                     DEKNGAAGSKINLFEVPFTFRTEYEGGVMEEACRRMLSDFFLKLREKRKEEKQ"
     gene            complement(992..>2002)
                     /locus_tag="ACZPE2_03890"
     CDS             complement(992..>2002)
                     /locus_tag="ACZPE2_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1319023.1"
                     /translation="GGNYPPPPPYNWQYDPLAQEKKREKKNISRISIAVGLAVIGFTL
                     LSMVLGVLLRAIPGFLSEYKGNASFSIYFDMVYSVFIIGVPFFAAMSYLKSKGALPAV
                     LPLGTAKNTPVFFLLVFSGLMACIAGSYASSIFGSIFQNLFGIEFTMAEDGIKLTTAS
                     VILPYIVKTAVLPALIEEFAMRGVVMQSLRKYGDWFAIIMSSLVFALLHGNMVQIPFA
                     FIAGIAIGYAVTVTGSMWTGVLIHFLNNLASIIMQIGIDNCSETVSAVITMSVVFVIM
                     ISGIICAAIYFKNYSHAYPLKNGESLVLSWNEKTRAFVVTAPMIIAICLLLWETAMYI
                     EF"
BASE COUNT          509 a          545 c          442 g          506 t
ORIGIN      
        1 ttgtgacaat tataataccg tctacgggaa gactttggaa taccgtcagc ggaacgtcgc
       61 ccgtgccggg gggcatatcg acgaacatat agtcaacgtc gccccagaga acctcctgcc
      121 agaactgatt tataacgccc gaaatgacgg ggccgcgcca tactaccgga gcgtcctcct
      181 tttcgagcag cagatttatg ctcattatct ttattcccgt cttgctctcg accgggaaca
      241 ttccctcgtc gctgcccatg gcgcgctcgt gtataccgaa tgccttgggg attgacggac
      301 ctgtgacgtc ggcgtccata accgccgtct caaagcccct cgcctgtgaa gccgaggcaa
      361 gaagcgatgt cacaaggctc ttgcctacgc cgcccttgcc gctgactacc gctataacct
      421 tttttatatt gctgtaatcg ttttgacgaa gcttcatatc ctctgctttt ctctcgctgc
      481 agttctcacc gcaggtagaa cagtcatgtg tgcaaccttc gctcattgtt tttcctcttt
      541 tcttttttct cgaagcttca aaaagaagtc cgagagcatt ctcctgcaag cttcctccat
      601 cacgccgccc tcatattcgg tacggaacgt gaacggaacc tcaaacaaat ttatttttga
      661 ccctgcggcg ccgttttttt catcgtatgc gccgaaggtc actttttcga ttcttgaatt
      721 tataatcgca ccggcgcaca tggggcacgg ctcaagtgtg acatacattt cacatctcgg
      781 aagtctccag cccgaaagcg cacggcaggc accgtcaatc gcctcgattt ctgcgtggca
      841 cagggcgttt ttttcctttt cgcggcggtt tctgcccgtt gaaacaacct cgccgtctct
      901 tactatgacc gcgccgacgg gcacctcgcc gtcaagaaac gccttttcgg cttcgagcag
      961 ggcaagacgc ataaaatcat ttttatccat atcagaactc aatatacatg gcagtttccc
     1021 acaagagcag acaaatcgct attatcatgg gtgccgtcac cacaaaggct ctcgtttttt
     1081 cattccatga gaggacgaga ctctcgccgt ttttcagcgg ataagcgtgc gaataatttt
     1141 tgaaatatat cgcggcgcat ataatgccgc ttatcattat cacaaagaca acggacatcg
     1201 taattactgc cgacacggtt tcggagcagt tatcaatgcc tatctgcata attatcgacg
     1261 caagattgtt gagaaaatgt atcaaaacgc ccgtccacat actgcccgtt accgtaacgg
     1321 catagcctat ggcaatacct gcgataaagg cgaacggaat ttgaaccata ttgccgtgca
     1381 aaagcgcaaa gacaagcgac gacataataa tcgcaaacca atcgccgtat ttgcgaagcg
     1441 actgcataac tacgccgcgc attgcgaact cctctatgag agccggcaaa acagcggttt
     1501 ttacgatata cggcaatata acggacgccg ttgtcagctt tatgccgtcc tccgccatgg
     1561 taaattctat gccgaagagg ttttgaaaga tacttccgaa aatgctcgac gcatagctgc
     1621 cggctatgca cgccataagt cccgaaaaaa caagcaggaa aaacacgggt gtgtttttgg
     1681 ctgttccgag cggcaaaacc gcgggcagag cgcccttgga ttttaaatat gacattgccg
     1741 cgaaaaacgg cactcctatt ataaaaacgg aatataccat atcaaaatat atgctgaagg
     1801 acgcgttgcc cttatattcc gacaaaaagc ccggtattgc tcgcaaaagc acgcctaaaa
     1861 ccatacttaa aagcgtaaag ccgataaccg caagtcccac ggcaatgctt atgcgggaaa
     1921 tatttttctt ttccctcttt ttctcctgtg cgagtgggtc atactgccaa ttatacggcg
     1981 gtggaggcgg atagtttccg cc
//
LOCUS       DBMDVO010000215         1991 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27352_length_1991_cov_0.905291, whole genome shotgun sequence.
ACCESSION   DBMDVO010000215 DBMDVO010000000
VERSION     DBMDVO010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27352_length_1991_cov_0.905291"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="ACZPE2_03895"
     CDS             complement(<1..490)
                     /locus_tag="ACZPE2_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1319024.1"
                     /translation="MDEEAKKYHKTSVGGQALIEGVMMRGPKGSAMAVRLPNGEIETT
                     YKNVKPWRDKCKFFGVPLIRGIVGFVESLVTGYGYLMESAEKSTQGLTEDEQDMSKFD
                     RWLNDRFGPKMMNVIGVISSVLGFGIAFFLFMWLPSWLIDLVTGGNLQKFHPLFEGIV
                     RII"
     gene            complement(505..1923)
                     /locus_tag="ACZPE2_03900"
     CDS             complement(505..1923)
                     /locus_tag="ACZPE2_03900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1319025.1"
                     /translation="MAKKSITARWVLNTLCAIIFVLFCIIIVVSVLFKEQYYETVRVT
                     LDSRATNVVSSYFSISNDSSDVAFNLRAKEFVEDFSDKSVMEVWLIDRSGNVVVSSSG
                     FSVENEKYPDYAEAKLSDTGKATWVGRMSSGEKVMALSYMLPSSGGQNSGAVRYVVSL
                     SDIDSQLIKVILLMCFIALVIIAFVTVSGLFFVRSIVRPVKKINETAIKIAKGDLGAR
                     IEDTGGDDEIGQLCESINNMAHEIKESERMKNEFISTVSHELRTPLTAIKGWGETILE
                     DPIKDEALTKRGLNVIISESERLTSLVEELLDFSRMENGNLTMRMSSMDVLAELDEAV
                     FVFKDRSMREGKELLYNVSEVPAPMTGDPNRIKQVFVNILDNSFKYTEAGGTISVTAK
                     AENGFVSITIADTGCGIPTADIPYVTEKFYKANASVRGSGIGLAVVNEIVKRHNGTLS
                     LNSIEGKGTTVTVVFPIQQTQQ"
BASE COUNT          531 a          505 c          396 g          559 t
ORIGIN      
        1 ttattattct cacaataccc tcaaaaagag gatgaaattt ttgaagattg ccgcccgtca
       61 caaggtcgat aagccacgac ggaagccaca taaacaggaa aaatgcgatc ccaaatccga
      121 gcacggagga aataacgcct atgacattca tcattttggg cccgaaacgg tcattaagcc
      181 agcggtcgaa tttcgacata tcctgctcgt cttcggtaag accctgagtg gatttttcag
      241 ccgactccat aagatacccg tagccggtta cgagtgattc gacgaacccg acaatgcctc
      301 tgattaacgg aacaccgaag aatttgcact tgtcgcgcca cggctttaca tttttatatg
      361 ttgtttcgat ttctccgtta ggcagtctta ccgccatggc agagcctttc ggtccgcgca
      421 tcattacgcc ctctataaga gcttgaccgc caacggaggt tttatgatat ttcttagctt
      481 cttcgtccaa aattattctc ctttttattg ctgagtttgc tgtatcggaa acacaactgt
      541 cacggtggtg cccttaccct caatactgtt gagagagagc gtgccgttgt gcctttttac
      601 aatttcattt accaccgcaa gaccgatgcc cgagcctctg acggacgcat ttgccttata
      661 gaatttctct gtgacataag gaatgtccgc agtggggatc ccgcagccgg tatctgctat
      721 cgttattgaa acaaagccgt tttccgcctt tgccgtaaca cttatcgtgc cgcccgcctc
      781 ggtatattta aaggaattat caagtatgtt cacaaatacc tgcttaattc gattcgggtc
      841 gcccgtcata ggagccggaa cctcggaaac gttatataac agctccttgc cctcacgcat
      901 agagcggtcc ttaaaaacga ataccgcctc gtcaagctca gccaaaacgt ccatggatga
      961 catacgcata gtcaaattgc cgttttccat tcttgaaaaa tcgagaagct cctcaacaag
     1021 acttgtaagc cgctcagatt cgcttattat gacattgagt ccgcgttttg taagcgcctc
     1081 atcctttatc gggtcctcaa gtatcgtctc gccccacccc tttatagctg tcagcggagt
     1141 acgaagctca tgtgatacgg ttgaaataaa ctcgtttttc atacgctcgc tctcttttat
     1201 ttcatgcgcc atattgttta tggactcaca gagctgacct atttcatcat ctccgccggt
     1261 atcctctata cgtgcgccga ggtctccctt tgcaattttt attgccgttt cgttgatctt
     1321 tttaactggc cttactatcg aacgcacaaa gaaaagcccc gatacagtaa caaaagctat
     1381 tataacaaga gcaataaaac acataagaag tattaccttg ataagttgcg agtcaatgtc
     1441 ggaaagagaa acaacatatc gaacggctcc ggaattttgc ccgccggacg atggtagcat
     1501 atatgaaaga gccataacct tttcaccgga gctcattctg ccaacccatg tagccttgcc
     1561 ggtatcggat agcttagcct cggcgtaatc cggatatttt tcgttttcga cagaaaatcc
     1621 gctggaggat accacgacgt taccgcttcg gtctattagc caaacctcca taacggattt
     1681 atcgctgaag tcctcgacaa attccttagc acgaagatta aacgcgacgt cggagctgtc
     1741 atttgaaata ctgaaatatg atgaaacgac atttgtggct cttgaatcaa gtgtcacccg
     1801 cacagtctcg taatactgct ctttaaacag aaccgagacg actatgatta tacaaaaaag
     1861 cacaaaaata atggcacaga gtgtatttaa aacccagcgt gcggttatac tcttttttgc
     1921 cacgtttttt tcgctccttt cgttctttta ttctatccac ttatagcctc tgccccaaat
     1981 ggtaataaga t
//
LOCUS       DBMDVO010000216         1988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27392_length_1988_cov_1.255432, whole genome shotgun sequence.
ACCESSION   DBMDVO010000216 DBMDVO010000000
VERSION     DBMDVO010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27392_length_1988_cov_1.255432"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="rpsC"
                     /locus_tag="ACZPE2_03905"
     CDS             complement(<1..564)
                     /gene="rpsC"
                     /locus_tag="ACZPE2_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1319026.1"
                     /translation="MGQKINPTGFRVGVIKDWESRWYAGKKDFGNTLISDYELRNYLL
                     STLAPAGVPKVEIERDPKRVRINIHCAKPGMVIGKGGAEIEKLKDVCKAKLGEDKDVF
                     INIVEIKQPDLDSQLVAENIAGQLERRVSFRRALKQAIGRTMKAGAKGIKVTTSGRLG
                     GAEIARSEKYSEGNVPLHTLRANIDYGF"
     gene            complement(584..916)
                     /gene="rplV"
                     /locus_tag="ACZPE2_03910"
     CDS             complement(584..916)
                     /gene="rplV"
                     /locus_tag="ACZPE2_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1319027.1"
                     /translation="MEATAKATHVRIAPRKVQIVLDLIRNKPCDEALAILKYTPKAAC
                     EPLEKLLKSAMANAENKDMDTSSLFVSECKVDQGPTLKRIRPRAQGRAYRINKKTSHI
                     TLVVKEAE"
     gene            complement(941..1213)
                     /gene="rpsS"
                     /locus_tag="ACZPE2_03915"
     CDS             complement(941..1213)
                     /gene="rpsS"
                     /locus_tag="ACZPE2_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1319028.1"
                     /translation="MSRSTKKGPYVAPKLLRKVEEMNKNGEKIVLKTWSRSSTIFPDF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFRGHSGSKTTN"
     gene            complement(1233..>1988)
                     /gene="rplB"
                     /locus_tag="ACZPE2_03920"
     CDS             complement(1233..>1988)
                     /gene="rplB"
                     /locus_tag="ACZPE2_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1319029.1"
                     /translation="KVAPERSLLEPLSKNAGRNSYGRITVRHRGGGNRRKYRVIDFKR
                     DKAGEATVLTLEYDPNRSSHIALIQYADGEKRYILAPVGLKVGDKVEAGADADIKTGN
                     ALPLTNIPTGTVVHNVELYPGRGGQLARSAGNSAQLMAKEGVYALLRLPSGELRNVPA
                     TCMATVGTVGNTDHENVKLGKAGRTRHLGIRPTVRGSVMNPNDHPHGGGEGKAPVGRP
                     GPCTPWGKPALGYKTRAKKNRSDKLIVKRRNSK"
BASE COUNT          399 a          506 c          501 g          582 t
ORIGIN      
        1 aaaaccgtag tcaatgttag ctcttaaagt gtgtagagga acatttcctt cgctgtattt
       61 ttcgcttctt gcgatttcag ctccgccaag tcttcctgat gtagtaacct tgataccctt
      121 tgctccggct ttcattgttc tgccgatagc ctgtttaaga gcgcggcgga aagaaactcg
      181 tctttcaagc tgacctgcga tattctctgc tacaagctga gaatcaaggt cgggctgctt
      241 gatttctacg atattgatga aaacgtcttt gtcttcaccg agttttgctt tgcatacatc
      301 cttgagcttc tcgatctcag caccgccctt accgataacc ataccgggct tagcgcagtg
      361 aatgttgatg cggactctct tgggatctct ttcgatttcg accttcggaa cgcctgcggg
      421 agcaagagtg ctgagtaggt agttacgaag ctcatagtcc gaaataagag tgttgccgaa
      481 gtcctttttg ccggcgtacc agcgggactc ccaatccttt ataacgccga cacggaagcc
      541 ggtaggattt attttctggc ccattttatt acctcctcct tacttactct gcttctttaa
      601 ccacaagggt tatatgcgag gttttcttgt ttattctgta tgcgcggccc tgtgctctcg
      661 gacgaatacg tttaagagtc ggaccctggt ctaccttgca ctccgaaacg aagagcgatg
      721 aggtatccat atctttgttt tctgcattag ccattgcaga cttcaaaagc ttttcaagcg
      781 gttcacacgc agccttcggt gtgtacttga gaatagctaa tgcttcgtcg caaggcttat
      841 ttctgataag atcaagtacg atctgcacct tacggggagc gatgcgtacg tgagttgcct
      901 tagcagttgc ttccattagt taatacactc ctttcgctga ttagtttgtt gtctttgatc
      961 cgctgtgacc tctgaaggtt cttgtcggag caaattcacc aagcttgtga ccaaccatat
     1021 cctctgtaac ataaaccgga acgtgcttgc ggccgtcgtg aactgcaaac gtgtgtccga
     1081 cgaagtccgg gaatatagtc gagctgcggc tccatgtttt gagaacgatc ttttcgccgt
     1141 tcttgttcat ttcttcaacc ttgcgaagca gtttcggtgc aacgtaaggg ccttttttag
     1201 tacttctgct cataatctac actcctttcg acttacttgc tgttacggcg cttaactata
     1261 agcttatccg aacggttctt cttagctctg gtcttataac cgagagcggg cttgccccac
     1321 ggtgtgcagg gaccgggacg tcctaccgga gccttacctt caccaccgcc gtgcgggtgg
     1381 tcgttcgggt tcattacaga accgcggact gtcgggcgga taccgaggtg acgggtacgt
     1441 cctgccttac cgagcttgac attttcgtgg tcggtgttgc cgactgtgcc tactgttgcc
     1501 atgcaggttg cgggaacatt tctgagctcg cccgagggta aacgaaggag cgcatatacg
     1561 ccctctttag ccataagctg agcggagtta cctgccgaac gtgcgagctg accgccgcgt
     1621 ccgggataaa gctcaacgtt gtgaactacg gtaccggtcg ggatatttgt aagaggaagt
     1681 gcgttgccgg ttttgatgtc ggcgtctgcg cctgcctcaa ccttgtcgcc taccttgagg
     1741 cctaccggag cgaggatgta tctcttctcg ccgtcggcat actggataag agcgatatga
     1801 gacgaacggt tggggtcgta ctcaagtgtg aggactgttg cctcgcctgc cttatctctc
     1861 ttgaagtcga tcacgcggta ctttctgcgg tttccgccgc cgcgatgacg gactgtgatt
     1921 cttccgtagc tgtttctgcc ggcgttcttt gaaagaggct ctaagaggct tctctcggga
     1981 gctacctt
//
LOCUS       DBMDVO010000217         1984 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27469_length_1984_cov_1.149761, whole genome shotgun sequence.
ACCESSION   DBMDVO010000217 DBMDVO010000000
VERSION     DBMDVO010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_1984_cov_1.149761"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="ACZPE2_03925"
     CDS             <1..562
                     /locus_tag="ACZPE2_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319030.1"
                     /translation="RDCDIYIPLSSCEKFNGVIDGTVTPVPLKGLTKIGFLLKTFTAL
                     TDRLSEVMQPSEEALKDRAFFELSTKLTFYTISVALSQIGNQDKIGQASASYMLDGDI
                     AFCIKDGPAATIRVKDHHLVTIKEYPKKPRAIMQFDTIDLAYDLFNGKVNSLECIGKG
                     TVEIRGMLSMVDNMNRILDRVALYLA"
     gene            577..1845
                     /locus_tag="ACZPE2_03930"
     CDS             577..1845
                     /locus_tag="ACZPE2_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1319031.1"
                     /translation="MSKFPEYKHTKSHEYFDRAIKVIPSGIYGHLGPAEGLWVPVSKW
                     PFYSEKAKGTYFWDVDGNKYIDYMCAYGPNVLGYNDPDVDAAAMKQMKLGNCTTSPSK
                     VMVECAENLVNTVASADWAFFAKNGSDVTTLAVMTARAHTHKKKMFFLKGYYHGDFQW
                     GQKIDYPGILPEEVANNVVVPWFDLQALEDAYNACDGDVAGLIAQPYDHGNFYDNRCA
                     SKEYWQAVRKFCDDHGMVLIFDDVRTGFRLDIAGSDHYYGIKADLICFCKALANGYNM
                     SALCGGEHMKATVSGVTYTGSYWMSAIPFAASIACIDKMKKLNTPKMFREMGTKLTEG
                     FKAAGKEHGFDLVVTGEPALFYLRIANDDSLMLHQEWISECVSRGLFLASHHNHFMNA
                     AVTDEDIKKSIEIAEDAFSVVEKNHPELKG"
BASE COUNT          534 a          494 c          471 g          485 t
ORIGIN      
        1 gagagattgc gatatttaca taccgctttc ttcctgcgaa aaattcaacg gcgtaattga
       61 cggaaccgtt actcctgtac cgcttaaggg tcttaccaag ataggtttcc ttctcaagac
      121 ctttacggcc cttaccgacc gactttcaga agtaatgcag ccgagcgaag aggctcttaa
      181 ggacagggcg ttttttgagc tttcgactaa gcttacattc tacaccattt ccgttgcgct
      241 gtctcagata ggcaatcagg ataaaatcgg ccaggcaagc gcttcttata tgcttgacgg
      301 cgatatagca ttttgcatta aagacggccc cgccgcaaca attcgcgtta aggatcatca
      361 ccttgtaaca atcaaggaat atccgaaaaa accgagagct attatgcagt tcgacaccat
      421 tgaccttgct tacgatctct tcaacggcaa agttaattct cttgaatgta taggtaaggg
      481 taccgttgag attcgcggca tgctttccat ggtagacaat atgaacagaa tactcgacag
      541 agtagctctg tatttagcat aaggagggcg ataaatatga gtaaattccc ggaatacaaa
      601 cacactaaga gccatgaata ctttgacaga gccattaagg taataccctc cggtatttac
      661 ggtcacctcg gccccgcaga gggtctttgg gttcccgttt caaaatggcc tttctattcc
      721 gaaaaagcaa agggtacata tttttgggac gttgacggca acaagtacat agattatatg
      781 tgcgcttacg gccccaatgt gctcggctat aacgaccccg atgttgatgc tgccgctatg
      841 aagcagatga agctcggcaa ctgcacaacc tctccgtcaa aggttatggt tgagtgtgcc
      901 gaaaatcttg taaataccgt agcttccgca gactgggcat tctttgcaaa gaacggctcc
      961 gacgttacaa cgcttgcggt tatgaccgct cgtgcacaca cccacaagaa aaagatgttc
     1021 ttcttaaagg gctattatca cggcgacttc cagtggggtc agaagataga ctaccccggc
     1081 attcttcccg aagaggttgc aaacaacgtt gttgttccgt ggtttgatct tcaggctctt
     1141 gaggacgctt acaacgcttg cgacggcgac gttgcaggcc tcatagctca gccgtacgat
     1201 cacggcaact tctacgacaa ccgctgcgcc tccaaggagt attggcaggc tgttcgtaag
     1261 ttctgcgacg atcacggtat ggttcttatc tttgacgacg taagaacggg cttccgcctt
     1321 gatatagccg gctcagacca ttattacgga atcaaagccg accttatctg cttctgcaag
     1381 gctcttgcaa acggatacaa tatgtctgct ctctgcggcg gcgagcatat gaaagccacc
     1441 gtttcaggcg ttacatacac aggctcttac tggatgagcg caatcccctt cgccgcttcc
     1501 atagcctgca tcgacaagat gaaaaagctt aacacaccca agatgttccg cgaaatgggc
     1561 acaaagctta ccgagggctt taaggctgcc ggtaaagagc acggctttga ccttgtggtt
     1621 accggtgaac cggcactctt ctatcttcgt atcgcaaacg atgacagcct tatgcttcat
     1681 caggagtgga tatccgagtg cgtaagccgc ggcctgttcc tcgcgtcaca ccacaaccac
     1741 tttatgaatg ccgccgttac cgacgaggac atcaaaaagt ccatagagat agctgaggac
     1801 gcattctcgg tagttgagaa aaatcacccc gagctcaagg gctgatttca aacacggatt
     1861 taaggcggct gtgcgtggag cgcggccgcc cgtaatacaa aactttacat tcagggagga
     1921 aaattatgga aagagcacct ttgaccaagg aggaatggct cgcgcttgag aaaaaagcca
     1981 atga
//
LOCUS       DBMDVO010000218         1980 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27559_length_1980_cov_1.370942, whole genome shotgun sequence.
ACCESSION   DBMDVO010000218 DBMDVO010000000
VERSION     DBMDVO010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27559_length_1980_cov_1.370942"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>493)
                     /locus_tag="ACZPE2_03935"
     CDS             complement(<1..>493)
                     /locus_tag="ACZPE2_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319032.1"
                     /translation="KINASGNRVLSVIADTENGEKEFTGDIFFSSMPVKDLVGGISGI
                     EIPETVKSAAANLPYRDFVTVGLLVDRMLLKNKTDIKTVNDIVPDCWIYVQDKNVKLG
                     RIQIFNNWSPYMVKEPENTVSLGLEYFCDEGDDFWNMPEEECVALASDELVRIGVIDC
                     GAVL"
     assembly_gap    494..503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<504..1293)
                     /locus_tag="ACZPE2_03940"
     CDS             complement(<504..1293)
                     /locus_tag="ACZPE2_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1319033.1"
                     /translation="MKKVIIIGAGPAGITAALELLRRGKNEYDVTVLEESDSIGGISR
                     TVNLGGNRMDIGGHRFFSKEQRVNEWWQSILPLQGAPSKDDKLLKRKAHLVKGGADPE
                     KTDKVMLYRRRVSRIYYLNKFFDYPVTMKMGTLINLGLLRTLEAGFSYLFAMMFKKPE
                     TSLENFYINRFGKKLYSMFFEDYTEKLWGRHPRDISADWGSQRVKGLSIKEVLSSVLH
                     KGKSDETSLIEEFKYPKLGPGELWEAAAGEFTERGGKILKNVSVK"
     gene            complement(1317..>1980)
                     /locus_tag="ACZPE2_03945"
     CDS             complement(1317..>1980)
                     /locus_tag="ACZPE2_03945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1319034.1"
                     /translation="YNVKNGQRLIRQEDSVMRIIDISNDILSAEVYEGDPVPELISLA
                     SLKNGDRYNLNAVNMGLHNGTHMDAPLHFIDGADGIDKVKPDAFIGPCTVLEVSPGII
                     TGSVVEEYFPRRAERILLKSGGRAFIHRSAADAMAYFGYKLVGTDGLDVEPPQSESYE
                     THKALLGQNIAVLEGLDLSDVANGEYFLIAPPLKIESAEASPVRAMLITDYVFWSGKP
                     EA"
BASE COUNT          478 a          547 c          404 g          541 t
ORIGIN      
        1 ttaaaaccgc tccgcagtcg ataacaccga ttctgacaag ctcatcggac gcgagcgcta
       61 cacactcttc ctcgggcata ttccaaaaat cgtcgccctc gtcacagaag tattcgagcc
      121 ccaaagatac ggtgttttcc ggctccttca ccatataggg tgaccaattg ttgaatatct
      181 gtattctgcc gagctttacg tttttatcct gaacgtatat ccagcagtcc ggtacaatgt
      241 cgtttaccgt cttaatatcc gtcttatttt taagaagcat acggtcgacg agaagtccga
      301 ctgttacaaa atcacggtac gggagattcg ccgccgcact tttcaccgtt tcggggattt
      361 cgattccgga tattccgcct accaagtcct ttacgggcat agacgaaaag aatatatcac
      421 ccgtaaattc cttttcgccg ttttccgtat cggcgattac gctcaaaacc ctgttacccg
      481 aagcatttat cttnnnnnnn nnnttttcac ggagacattc ttcaatattt taccgcctct
      541 ttcggtaaat tcaccggctg cagcctccca cagttctccc ggcccgagct tcggatattt
      601 aaattcctca ataagcgatg tttcgtcgct cttacccttg tgcaaaacag atgacaaaac
      661 ctcttttatc gagagcccct ttacacgctg cgagccccaa tccgccgaaa tgtctctcgg
      721 atgtctgccc caaagctttt cggtatagtc ctcaaaaaac atcgaataca gctttttgcc
      781 gaaacggttt atatagaaat tctcaagaga ggtttcgggc tttttgaaca tcatcgcaaa
      841 aaggtagctg aagcctgcct cgagcgttct taaaagcccg agatttataa gcgttcccat
      901 tttcatcgtc acgggataat cgaaaaattt attaagatag tatatgcgcg acaccctgcg
      961 gcgatagagc attaccttat cggtcttttc ggggtcggca ccgcctttta cgagatgcgc
     1021 cttgcgcttt aggagcttat cgtcctttga cggagcaccc tgaagcggca aaatactctg
     1081 ccaccactca tttacgcgct gctcttttga aaagaacctg tgaccgccta tatccatacg
     1141 gttgccgcca agattcaccg tgcgcgaaat tccgcctatc gagtcgctct cctcgagcac
     1201 cgttacatca tattcgtttt tgcctctgcg aagaagctcg agagccgccg ttatgccggc
     1261 aggtccggct cctataatta taactttttt caaatgatta aaacctttcc cggaatttaa
     1321 gcttcgggct ttccgctcca aaacacatag tcggttataa gcatagctct tacgggtgac
     1381 gcctctgccg actctatttt taacggcggc gcaatcaaaa agtattctcc gtttgcaaca
     1441 tccgataagt caagtccctc aaggacagct atattttgcc cgagaagcgc cttatgagtt
     1501 tcatagcttt cgctctgcgg cggctcgacg tcaagcccgt ctgtaccgac aagcttatac
     1561 ccgaaatatg ccatggcgtc ggcggcactg cgatgtataa acgccctgcc tcccgatttc
     1621 agaagtatac gctccgccct gcgcggaaaa tactcctcaa ccacggagcc ggttatgatt
     1681 cccggcgaaa cctcaagcac tgtgcacggc cctataaagg cgtcgggctt taccttgtcg
     1741 attccgtcgg caccgtcgat aaaatgcagc ggcgcgtcca tatgagtacc gttgtgaagc
     1801 cccatattga cggcgttgag attgtatctg tccccgtttt tcagggaagc cagactaata
     1861 agttcaggca ccggatcacc ctcgtaaacc tctgccgaca aaatatcgtt ggaaatatct
     1921 attattctca taacagagtc ctcctgcctt attaaccgtt gcccgttttt tacattatac
//
LOCUS       DBMDVO010000219         1972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27702_length_1972_cov_1.211117, whole genome shotgun sequence.
ACCESSION   DBMDVO010000219 DBMDVO010000000
VERSION     DBMDVO010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27702_length_1972_cov_1.211117"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            95..463
                     /gene="rpsM"
                     /locus_tag="ACZPE2_03950"
     CDS             95..463
                     /gene="rpsM"
                     /locus_tag="ACZPE2_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR1319035.1"
                     /translation="MASISGVDLPRDKRVEIGLTYIYGIGRKTASDIIAATGVNPDTR
                     VKDLTEEDVAKLREYIDHHVKVEGDLRRDTAFDIKRLIEIGSYRGIRHRKGLPVRGQT
                     SKNNARTRKGPKKTIANKKK"
     gene            479..883
                     /gene="rpsK"
                     /locus_tag="ACZPE2_03955"
     CDS             479..883
                     /gene="rpsK"
                     /locus_tag="ACZPE2_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1319036.1"
                     /translation="MATKATAKGAASRKRRERKNIDKGAAHIQSTFNNTIVTITDVQG
                     NAVSWASSGEMGFRGSKKSTPFAAQTAAETAAKVAVDHGMKTVEVYVKGPGSGREAAI
                     RALQTAGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            902..1501
                     /gene="rpsD"
                     /locus_tag="ACZPE2_03960"
     CDS             902..1501
                     /gene="rpsD"
                     /locus_tag="ACZPE2_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1319037.1"
                     /translation="MAKNTEPVAKRCKALGISPAVMGYTNKETTRNSYTKGRRKKSEY
                     AMQLNEKQKVKFIYGILEKQFHSYYEKAAKAQGQTGVVLLTMIETRLDNVAFRTGFCK
                     TRREARQAVSHGHFTVNGKRVNIPSYLVKAGDVIAVAETSKDNARFKAVKEEGVFGVS
                     QKWLDVNAAELTAKVVALPEREDIDYPVEEHLIVEFYSK"
     gene            1581..>1972
                     /locus_tag="ACZPE2_03965"
     CDS             1581..>1972
                     /locus_tag="ACZPE2_03965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1319038.1"
                     /translation="MIGIDKPSIEALDLSEDGTYGKFVMEPLERGYGTTIGNSLRRVL
                     LSSLPGYAITSAKIDGVLHEFSTIPGVKEDVTEIVLNLKGVILKIHGDGPKTIYIEAS
                     GKCTVTAGDIKTDSDVEILNPDHLIATLS"
BASE COUNT          590 a          454 c          480 g          448 t
ORIGIN      
        1 aaagatctaa acaacgatgc ttgctctttt tgtaaaagct ttttcaatgg cggtcagagt
       61 tgccgccgca tcaaaaatgg aggtgcaact gtaaatggca agtatttcag gtgttgattt
      121 accgagagat aagagagtcg aaatcggcct cacatacatc tacggtatcg gcagaaaaac
      181 tgcaagcgat attatcgcag caacaggtgt aaatcccgac actcgtgtaa aggatttgac
      241 cgaagaagat gtcgctaagc ttcgtgagta tatcgaccat cacgtaaagg tagagggcga
      301 ccttcgccgc gatacagcat ttgacatcaa gagacttatc gaaataggtt cataccgcgg
      361 tattcgtcac cgcaagggct tgcccgtaag aggtcagacc tctaagaaca acgcccgtac
      421 acgcaagggt ccgaagaaga ccattgcaaa taagaagaaa taagggaggg attagtaaat
      481 ggctaccaaa gcaacagcaa aaggcgctgc ttctcgcaag cgtcgtgaac gcaaaaatat
      541 cgacaaaggt gcagctcata ttcaatccac cttcaataac actatcgtga ccatcaccga
      601 cgtacaaggt aatgcggttt cttgggcaag ctcaggcgaa atgggcttca gaggctctaa
      661 gaaatcaact ccgttcgcag ctcagactgc tgccgagacc gctgctaagg ttgcggttga
      721 ccacggaatg aagaccgttg aagtttacgt caagggtccc ggttcgggac gtgaggcagc
      781 tatccgtgcg cttcagacag caggactcga agtaactatg attaaagacg ttactcctat
      841 tcctcacaac ggatgccgtc cgcccaagag aagaagagtt taattcggag gtgtaatttt
      901 tatggcaaag aatacggaac ccgtagcaaa gcgctgcaag gcgctcggta tttcaccggc
      961 cgttatgggt tataccaata aggaaactac cagaaattcc tatactaaag gccgccgtaa
     1021 aaagagtgag tatgctatgc agcttaacga gaagcagaag gttaagttca tctacggtat
     1081 tctcgaaaag cagttccact catattacga aaaggctgct aaggctcagg gtcagaccgg
     1141 cgttgtactt cttacaatga ttgagacccg ccttgacaac gtagctttca gaacaggttt
     1201 ctgcaagacc cgccgcgagg caagacaggc agtttctcac ggccatttca ctgtaaacgg
     1261 caagagagta aacatcccgt cataccttgt taaagcaggc gacgtaatcg cagttgcaga
     1321 gacaagcaaa gataatgcaa gatttaaagc cgttaaggaa gagggcgttt tcggcgtttc
     1381 tcagaagtgg cttgatgtca acgctgccga gcttacggca aaggtagtgg ctctccccga
     1441 gcgcgaggat atcgactatc ccgttgaaga gcatcttatc gttgagttct actcaaaata
     1501 ataggctcgc tgcattactt ttcatacgaa tacaaaccac agtcgggctt agcccgaacc
     1561 cacaattagg agggttttga atgataggaa ttgataagcc cagtattgaa gcactcgatt
     1621 tgtcggaaga cggcacctac ggcaagttcg taatggagcc gctcgaaaga ggatacggta
     1681 caacaatcgg aaacagtctc cgcagagttt tactgtcttc tcttccggga tacgccatca
     1741 catcagcaaa aattgacggc gtacttcacg aattttcgac cattcccggt gttaaagagg
     1801 atgtcacgga gattgttctt aatcttaagg gcgttatcct caagatacac ggcgacggtc
     1861 ctaagactat ctatatagaa gcgagtggca aatgcactgt tacggcaggc gacataaaaa
     1921 ccgattcgga cgttgaaata ctcaaccccg atcatcttat cgctacactg tc
//
LOCUS       DBMDVO010000220         1972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_27706_length_1972_cov_1.117050, whole genome shotgun sequence.
ACCESSION   DBMDVO010000220 DBMDVO010000000
VERSION     DBMDVO010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27706_length_1972_cov_1.117050"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="ACZPE2_03970"
     CDS             <1..1602
                     /locus_tag="ACZPE2_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319039.1"
                     /translation="NKRKATAKIIVFVIFLVVLTLFSSLKTMFADLSAFGNSEYAFLG
                     VNLMLLLIAALIDVGEIKKSFSLLGRKKITRSMCVTVALLAGILQCIAAFSGHGELPG
                     NIHIYAPAVFVPLIFTAVADKVRAKTDIADFEYIVKHPDELYSVSKIEDEGEAFEIGR
                     GLLLGDPDIRYSAKIGFPSKFVEMTLRRDPSDGVYALSLPAVLTAAVITAVVAGVISK
                     NLVYAVSAFTAVTLIGLPASSCLSAFDILKTTNKQLRRSGSLVSGYYAAEDASTANAV
                     VIDASDLFLYGGCSLHGFKLYNSMRIDDAFLYTAAVVIKSGGALSDVFDSVILSKRDI
                     LPPVESLVYEEKLGCSCWINNQRVLVGNRDLLSKHNVTPPSEDEEKKFLKSGRQVIYL
                     AVEGKTAAGFSVEYKPNGDIARYLNRLEKYGVSVLVRTTDPNITEELVEQYFDLPHGF
                     VKVISPVAGKMFKELYETVKPSGDCKVIHDGSVYTLLKSFAAAFLITDKFRLSSVLQY
                     IGVGIGILAVAAMAFTSGLSQAGAL"
     gene            1685..>1972
                     /locus_tag="ACZPE2_03975"
     CDS             1685..>1972
                     /locus_tag="ACZPE2_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1319040.1"
                     /translation="MIILAWIRDAAEWLWAISPVKYTIISIVRLCLAALLGGIIGYER
                     EHSHRPAGFRTHILVAVGSAVVMLTAVYMSERYPDKLDISRMPAQVVSGIGF"
BASE COUNT          463 a          448 c          523 g          538 t
ORIGIN      
        1 aataaacgaa aagcgaccgc aaagataatt gtttttgtga tttttcttgt ggttctcacg
       61 ctgttttctt cgcttaaaac catgtttgcc gatttgtcgg ctttcgggaa cagcgaatat
      121 gccttcctgg gcgtcaatct tatgctgctt ttgattgcgg cacttattga tgtcggagaa
      181 attaaaaaga gcttttcgct tctcgggcgg aaaaaaatta cgcgttctat gtgtgttacc
      241 gtagcgttgc ttgcaggcat tttgcagtgc attgcggctt tttcggggca cggggagctg
      301 ccgggcaata tccacattta tgcgccggcg gtatttgttc cgcttatatt tacggctgtc
      361 gcggataaag tgcgagccaa aaccgacatt gctgattttg agtacatagt gaagcaccca
      421 gatgagcttt acagcgtaag caaaatcgag gacgagggcg aggcctttga aataggcaga
      481 gggcttttgc tcggtgaccc cgatataaga tacagcgcaa aaatcggatt cccgtcaaaa
      541 tttgtcgaga tgacgcttcg ccgcgacccg tcggacggcg tttatgcgct atctctgcct
      601 gccgtgctta cggcggcggt gattacagcc gttgtagccg gagttatttc aaaaaatttg
      661 gtttacgccg tgtcggcatt taccgcagtc actcttatag ggcttccggc atcgtcgtgc
      721 ctgtcggcgt ttgacatact taaaacgact aataagcagc tcagacgctc gggttcgtta
      781 gtctccggct attatgccgc cgaggacgcc tcaaccgcaa atgcggtggt tatagatgcg
      841 tccgaccttt tcctctacgg cggatgcagc ttgcacggct tcaagctcta caattcaatg
      901 cgtatagacg acgcgttcct ctatacggcg gctgtcgtta ttaaaagcgg cggcgcattg
      961 tcggacgttt ttgacagcgt catactttca aagcgcgaca ttttgccgcc tgtggaatcg
     1021 cttgtctatg aggaaaagct cggctgctcc tgctggataa acaatcagcg cgtgcttgtg
     1081 ggtaaccgtg atttgctctc aaagcacaat gtgacaccgc caagtgagga tgaggaaaag
     1141 aaattcttga aatccggcag gcaggttatt tatcttgcag tagagggcaa aacagcggca
     1201 ggcttttcgg ttgaatacaa gccgaacggt gatatagcaa ggtacttgaa taggcttgaa
     1261 aaatacggtg tgagcgtgct tgtcagaacg actgatccga atatcaccga ggagcttgta
     1321 gaacagtatt ttgacctgcc tcacggcttt gtcaaggtca taagccctgt tgccggcaag
     1381 atgtttaagg aactgtatga gactgttaag ccgtcgggag attgcaaggt gatacacgac
     1441 ggcagcgtct atacgctgtt gaagtcgttt gccgccgcat tcctgataac cgacaaattc
     1501 aggctttcct ccgtccttca atatatcggc gtgggcatcg gaatacttgc cgttgccgct
     1561 atggcgttca cctcggggct cagtcaggcg ggggcgcttt agataataat gttcgtcctt
     1621 atttggactg cacttgtgat ttttataccg cggtttaaaa aaccgtaatt tatacggaaa
     1681 tgagttgata atattggcat ggataaggga cgcagccgag tggctgtggg cgataagtcc
     1741 tgtaaagtac acaattatat caatagtacg tttgtgcctt gccgcattac tcggcggtat
     1801 aataggctac gagcgcgagc attctcaccg ccccgccggc ttcagaactc atattcttgt
     1861 ggcagtcggc tcggctgttg ttatgctgac ggctgtatat atgtcagaga gataccctga
     1921 caagctcgat atttcaagaa tgcctgcaca ggtagtcagc ggcataggct tc
//
LOCUS       DBMDVO010000221         1957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28006_length_1957_cov_1.069504, whole genome shotgun sequence.
ACCESSION   DBMDVO010000221 DBMDVO010000000
VERSION     DBMDVO010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28006_length_1957_cov_1.069504"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="ACZPE2_03980"
     CDS             complement(<1..923)
                     /locus_tag="ACZPE2_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1319041.1"
                     /translation="MNNFFYSKLAVQNLKNNRKTYVPYILTCIFTTAMFFVVGTIANI
                     KWADSDALHSLLTFALATVGIFSAIFLFYTNSFLIKQRKKEFGLYNILGMEKRHIAKI
                     LFIETAYTYIFGTAAGIAIGALFSKLTFLLLLKILKFGGNIDFRFYQSTVDITALVFG
                     AIALLNLAHNLLCISLSNPVELLKGGNKGEKEPKAKVLTAVLGAAFLASGYTIALVVK
                     SPITAMGAFFAAVLLVIVGTFMLFSSGSIWILKALRKNKKYYYKAKHFTSVSSMIYRM
                     KQNAAGLASICILSTAVLVTVSTTVSLYAGT"
     gene            complement(916..1683)
                     /locus_tag="ACZPE2_03985"
     CDS             complement(916..1683)
                     /locus_tag="ACZPE2_03985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319042.1"
                     /translation="MPLLEVNNLKKVYTTRFGGAHVQALSNVSFSVEKGEYVAIMGES
                     GSGKTTLLNILASLDKPTSGEVLLNGRSLSAIREREISAFRRDNLGFVFQDFNLLDTF
                     SLKDNIFLPLVLSGKPYDEMAKRLKPLAEKLGITDILSKFPYEVSGGQKQRAAVARAL
                     ITNPQIVLADEPTGALDSKASDALLKLFGDFNEEGQTILMVTHSTKAASCANRIMFIK
                     DGEVFNQIYRGGMDNDEMFQKISDTLTAMATRGESNE"
BASE COUNT          574 a          438 c          390 g          555 t
ORIGIN      
        1 tccgtaccgg catacagcga taccgtagtg gacacggtga caagcaccgc ggtggaaaga
       61 atgcagatgc tcgcaagtcc tgccgcattt tgcttcatac ggtatatcat acttgaaaca
      121 gaagtaaaat gctttgcttt atagtaatat tttttatttt ttctgagagc ttttaatatc
      181 catatactgc ccgaggagaa aagcataaag gttccgacta ttacgagcaa aaccgccgca
      241 aagaacgcgc ccatcgccgt tatcggtgat tttacaacga gagctatggt ataacccgaa
      301 gctagaaagg ctgcacctaa aacagccgta agtacctttg ccttcggctc tttttcgccc
      361 ttatttccac cttttaaaag ttcaacggga tttgataggc tgatgcataa aagattatgt
      421 gcaagattca aaagagctat tgcgccgaat accaaagcag taatgtcaac tgttgactga
      481 taaaatctga aatcaatgtt tccgccgaat ttcagtattt taagcagcag caaaaaggtc
      541 agctttgaaa aaagcgcacc tatggcgatg cctgccgcag ttccgaaaat ataggtatag
      601 gcagtttcaa taaagagtat tttcgctata tggcgttttt ccataccgag aatgttgtat
      661 aatccgaatt cctttttacg ttgtttaata aggaagctgt ttgtgtaaaa taaaaatatg
      721 gcagaaaaaa taccgactgt tgcaagggca aacgtcaaga gggaatgcag agcgtcgcta
      781 tctgcccatt ttatgtttgc tattgtgccg accacaaaaa acatagctgt tgtaaatata
      841 cacgtcaaaa tatacggcac atatgtttta cgattatttt taagattttg aacggcaagc
      901 tttgaataga aaaaattatt cattgctctc acctctcgtt gccattgcgg tcagagtgtc
      961 cgaaatcttc tggaacatct cgtcgttatc catacctccg cgataaatct gattgaacac
     1021 ctcgccgtcc tttatgaaca taatcctgtt ggcgcaggac gccgcctttg tggagtgagt
     1081 aaccatcaaa atggtctgac cctcctcgtt aaaatcgccg aacagcttta aaagagcatc
     1141 agaagccttt gagtcaagcg caccggtggg ctcatcagcc aatacaatct gcggatttgt
     1201 tatgagagct cttgcgacgg cggcacgctg tttttgtccg cccgaaacct catatggaaa
     1261 ctttgagagt atatcggtaa ttcccaactt ttcggcaaga ggtttcaacc tctttgccat
     1321 ttcatcgtac ggcttgcctg aaagcacaag aggaaggaag atattatcct ttaaagagaa
     1381 tgtgtccaaa agattaaaat cctgaaatac aaatccgaga ttatcgcgtc taaacgccga
     1441 aatttctctc tctcttattg ctgatagact tctgccgttc aacagcacct ctccgctcgt
     1501 aggcttatca agcgacgcca aaatattaag caaggttgtc ttaccggaac ccgactctcc
     1561 cattattgca acatactcgc ccttttccac ggagaaactg acattggaaa gagcttgaac
     1621 gtgcgctccg ccgaaacggg tcgtataaac ctttttcaaa ttgtttactt ctaaaagcgg
     1681 cataataatt accgtagcct ttctgctaca aatattacat aaacaaccac ggttaaccct
     1741 tgctgtagcc gtacaaggcg tgacgggaat ttcgtctgtc taaaaataat aaccgaatag
     1801 cttttctttt tcggcgtgat tttacgtacc gaatgtatcc ctttgtttat tgcacgttca
     1861 gtatatcaaa acggagaatc gcaagccatc gaaaaggctt acaattcccc ttttaagctt
     1921 acatttatgt aaggtagcgt tattccactc cgaggct
//
LOCUS       DBMDVO010000222         1955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28041_length_1955_cov_1.002157, whole genome shotgun sequence.
ACCESSION   DBMDVO010000222 DBMDVO010000000
VERSION     DBMDVO010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28041_length_1955_cov_1.002157"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="ACZPE2_03990"
     CDS             <1..294
                     /locus_tag="ACZPE2_03990"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1319043.1"
                     /translation="DSVFDLEPGAFGIMEPNPSCEIMTAFDNGICIVPALVFSEDGYR
                     VGFGKGYYDRFLQSFSGTKIGLAYEEQIIKRIPHDSYDIKSDIIVSDKKVHRV"
     gene            312..1919
                     /gene="mltG"
                     /locus_tag="ACZPE2_03995"
     CDS             312..1919
                     /gene="mltG"
                     /locus_tag="ACZPE2_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1319044.1"
                     /translation="MDDNKRTPDDSYRDLLDIYAREEDEQKRPELKNMVERNRKSDKK
                     PFKLEIKDLDSEFTDAPQKRPPVRRDMPVHHSTDAPERHNAHNAHKRPPEKHKIHKRP
                     PEKNGTAPRGISYDDEFGPIITRGGRNGGNAASFGTASYEQASEQGAVRKRPPIKGIK
                     GNEKEIAVRIAAYFVRNKKTWITIAVCVVCAICLSSYLISCMNDVLAIRRDSENVISV
                     TIPAETNTSDVINILKDNGLIKHKHFCKMFAKIMNYRDDNYMSGIYYITKSMGVEKML
                     STFKSPPSTGETVRLSFPEGYTADQIVEKLEKYEVCSADAIYKAMREVDFSSEYTFIK
                     NEPNKEQRYRSLEGYLYPDTYDFYKGENASSVIRTFLNNFQKKWTDDYQKKADALNMS
                     VDDIIKLASIIEKEAADATQMPLVSSVLHNRLNKPGLYPSLQCDSTADYINDYIAKNV
                     TNATELAAYTSRYSTYKCEGLPVGAICNPGNDSINAALNPAKTDYYFFAHDTNKKIYL
                     AKNDSERQANNIAILQANQKAVKSASQ"
BASE COUNT          624 a          442 c          464 g          425 t
ORIGIN      
        1 gactctgttt ttgatcttga acccggggcg ttcggcataa tggagccgaa cccatcatgt
       61 gaaatcatga ccgcctttga taacggtata tgtatagtac cggctcttgt attttccgag
      121 gacggatacc gcgtcggctt cggaaaaggc tattatgaca ggtttttaca gtcgttttca
      181 ggcacaaaaa tcgggctcgc atacgaagag cagataataa agagaatccc gcacgacagt
      241 tacgatatta aatccgacat aattgtttcg gataagaaag tacacagagt ttaatgaaag
      301 gaggagcgcc catggatgac aataaaagaa ctcccgatga ttcgtatagg gaccttttgg
      361 atatttatgc gcgtgaggaa gacgagcaga aaagacccga gcttaaaaat atggttgaga
      421 gaaaccgtaa gagcgacaaa aagccattta agcttgaaat aaaagacctt gactccgagt
      481 ttaccgacgc tccgcaaaaa agaccgcctg tcagacgaga tatgcccgtg catcacagta
      541 ccgatgcgcc ggaaagacat aacgctcaca atgctcacaa aagaccgccg gaaaaacaca
      601 aaattcataa aaggccgccc gaaaaaaacg gtacggcacc gcgcggaata agctatgatg
      661 acgaattcgg tcccataatt acacgcggcg gaagaaacgg cggaaatgcc gcctctttcg
      721 gaacagcctc ttacgagcag gcgtcggagc agggtgccgt acgtaaaagg ccgccgataa
      781 aggggataaa gggaaacgaa aaagaaattg cggtaagaat agccgcgtat ttcgtaagaa
      841 acaaaaaaac gtggataacg atagcggttt gcgttgtatg cgcaatttgt ctgtcgtcgt
      901 atctgattag ctgtatgaac gatgttttgg caatacgccg cgacagcgaa aacgttatat
      961 ctgttacaat tcccgcagag accaacacaa gcgacgttat caacatatta aaggataacg
     1021 gccttataaa gcataagcat ttctgcaaaa tgtttgcaaa gataatgaat taccgtgatg
     1081 acaactatat gtcgggtatt tactatataa ccaagtcgat gggcgttgag aagatgctct
     1141 caacatttaa atctcctccg tcaacgggcg agaccgtaag gctttcgttc cccgagggct
     1201 acacggcaga ccaaatagtc gaaaagctcg aaaaatatga ggtttgctca gccgacgcca
     1261 tctataaggc catgagagag gtggacttca gcagtgaata cacctttatt aagaatgagc
     1321 cgaataaaga gcagcgttac aggagtcttg agggttatct ctaccccgac acatatgatt
     1381 tctataaggg cgaaaatgcc tcgagcgtta taaggacctt cctaaacaac ttccagaaga
     1441 agtggacgga cgattatcag aaaaaagccg acgcactcaa tatgtcggtt gacgatataa
     1501 taaagctcgc ctccattata gaaaaagagg ctgccgatgc gactcagatg ccgcttgtat
     1561 cgtcggtgct tcacaaccgt ctcaataagc cggggcttta tccgtcgctt cagtgcgact
     1621 ccacggcgga ctatatcaac gattatattg cgaagaatgt tacaaatgcc acggagcttg
     1681 ccgcctacac ctcacgttac agcacatata agtgcgaggg tctgcccgtg ggtgcgattt
     1741 gcaacccggg taacgactca ataaacgcgg cactcaatcc cgcaaagacc gattactact
     1801 tcttcgcgca cgataccaat aaaaagattt atcttgcgaa aaacgattcg gagcggcagg
     1861 ccaataacat tgccatactc caggccaatc aaaaggcggt caagtcggct tcgcagtaat
     1921 ttaccagagg agcaatatga aaagacgacc cgaat
//
LOCUS       DBMDVO010000223         1950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28119_length_1950_cov_1.437534, whole genome shotgun sequence.
ACCESSION   DBMDVO010000223 DBMDVO010000000
VERSION     DBMDVO010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28119_length_1950_cov_1.437534"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="ACZPE2_04000"
     CDS             <1..669
                     /locus_tag="ACZPE2_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1319045.1"
                     /translation="TTLPETTAVATEPKPAPTDPPKTSETAPQAKPTASTDFDTSFFD
                     DALFIGDSITTGLYLYGYLDESLVYAKLGLAPSTALESEIDGVTINSKIKANNPKKIY
                     IMLGTNSVGYSDGNYLANCMGELVQHISEITKAKVYVLSIPPITYYAEADYDNALTTS
                     AINKYNTALKSVIKGTKAKFLDFHSVLTAPDGYYYEEYHEMDGIHFMGSTYQVMLSFL
                     EKHS"
     gene            705..1268
                     /locus_tag="ACZPE2_04005"
     CDS             705..1268
                     /locus_tag="ACZPE2_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1319046.1"
                     /translation="MKSSITKRIMAGIVCAMVAVSICGCSEKPAQSSATESSVTSDAG
                     SVSESVSEASDSSTATGTAAEITAKIQEDVKFPGMAEIGADRMAGYYDVAADKIDSYS
                     AYICGSGAYPDEIAVFKMKSADDVNAVKSVLEKRVESQSATFKDYTPDEMYKIDGNNV
                     VTSGNYVALIICADNAKAIEDFKAMAK"
     gene            1294..1641
                     /locus_tag="ACZPE2_04010"
     CDS             1294..1641
                     /locus_tag="ACZPE2_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319047.1"
                     /translation="MAIECLVITFILGAIVFSFARADRIRWVIATVPLGILPLANCVS
                     CFVCTAFLGCELPKNIAAVILLLAVMASCVWIGIASGFLRTNRMRIPYISAGIGFNVV
                     LGLILLNFYIGLL"
BASE COUNT          586 a          433 c          437 g          494 t
ORIGIN      
        1 acgacactgc ctgaaactac agctgtagca acagagccaa agcctgctcc gaccgatccg
       61 ccaaaaacct ctgaaaccgc tccacaagcc aaaccgacag catcgacaga ctttgatacc
      121 tcattttttg acgatgcgct gttcataggc gacagcatta ctaccggtct ttacctttac
      181 ggttatcttg acgagtccct tgtctacgca aagctcgggc ttgccccctc aaccgcattg
      241 gaaagcgaaa tagacggtgt aaccataaac agtaaaataa aagcaaataa tcccaaaaag
      301 atatatatta tgctcggcac caacagcgta ggctattcag acggcaatta ccttgcaaat
      361 tgtatgggcg aacttgtaca acatatttca gaaatcacaa aagcaaaggt atacgtcctg
      421 tctatcccgc cgataacata ttatgccgaa gcagactatg ataacgcgct cacaacatct
      481 gcgataaata agtataatac cgcattaaaa agcgtaatca aaggcacaaa ggcgaaattt
      541 ctcgactttc attctgtgct gaccgctcca gacggatatt attacgagga atatcacgag
      601 atggacggaa ttcactttat gggttcaacg tatcaggtta tgctaagttt ccttgagaaa
      661 cattcttaat ataaaatgtg cataaaagaa aggaattatg aacaatgaaa agcagtatca
      721 caaagagaat aatggcagga attgtctgcg ctatggtcgc agtttcaata tgcggatgct
      781 ccgaaaaacc cgctcagtca tcggctacgg aaagctccgt cacatccgat gcaggctcgg
      841 tttccgagag cgtttccgag gcttctgaca gcagtacggc gaccggtacc gccgccgaaa
      901 tcacagctaa gatacaggaa gatgtcaagt ttcccggtat ggctgaaata ggcgcagacc
      961 gcatggcagg ctactatgac gttgccgccg acaagataga cagctacagt gcctatatct
     1021 gcggctcggg cgcttatccc gatgagattg cggttttcaa gatgaaatct gcagacgatg
     1081 taaatgctgt aaaatcggtt ctcgaaaagc gtgtagagag ccagagtgcc acattcaagg
     1141 actatacgcc cgatgaaatg tacaagatag acggcaacaa cgtagttaca agcggtaact
     1201 atgtagcgct tatcatctgt gccgacaacg caaaggcaat cgaagatttc aaggcaatgg
     1261 ctaagtaaat cagtaaaatg aaaggagaac cgtatggcta tagagtgtct tgttataacg
     1321 tttatacttg gggctatagt gttctcattc gccagagccg acagaatccg ctgggtcata
     1381 gcaaccgttc cgcttgggat actgccgctt gcaaactgcg tgtcctgctt tgtctgtact
     1441 gcgtttttgg ggtgcgagct gcctaagaat atcgctgcgg tgatacttct tcttgcggtg
     1501 atggcatcgt gcgtgtggat aggcatagcg tcgggttttc tgcgtacaaa ccgtatgcgg
     1561 ataccttata ttagcgcggg catcggcttc aatgtggttc ttgggcttat tctactgaat
     1621 ttctatatcg gattgttgta atccggatac gaaacggaga ttcacagctg taaacaaata
     1681 ccgcaccgcc gtaaatttct acggcggtat ttttatcttt acgttatatc cgcatacgtc
     1741 attcgtcaat agacaaattg tataaaaatg agcccggatt tttagttact tattcctaaa
     1801 agacatattg acaaatcgga gataacacta tataatatta attgtatttc gctcaccact
     1861 acatattgtg gttttacgac taaagcttaa acgagaggga gaaattaatg ctgactaaaa
     1921 ttcaaaagag ggacggacgt acagtaccgt
//
LOCUS       DBMDVO010000224         1949 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28142_length_1949_cov_1.256494, whole genome shotgun sequence.
ACCESSION   DBMDVO010000224 DBMDVO010000000
VERSION     DBMDVO010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28142_length_1949_cov_1.256494"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /gene="alsE"
                     /locus_tag="ACZPE2_04015"
     CDS             complement(<1..530)
                     /gene="alsE"
                     /locus_tag="ACZPE2_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose 6-phosphate 3-epimerase"
                     /protein_id="HIR1319048.1"
                     /translation="MEPMFNPSLMCMDLLDIKNQTEILNERCDLYHVDIMDGHFVKNI
                     TLSPDFVKAFSTIAKKPIDCHLMVTDPDDYIEVLAENGAGYICPHAETINTDAFRIMN
                     KIEALGCKKGVVLNPATPLSYIREYIDRIDKLTIMTVDPGFAGQPFIKEMLGKIREAK
                     ELKEKYGYKFLIEIDGS"
     gene            complement(557..1525)
                     /gene="alsC"
                     /locus_tag="ACZPE2_04020"
     CDS             complement(557..1525)
                     /gene="alsC"
                     /locus_tag="ACZPE2_04020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HIR1319049.1"
                     /translation="MSQSKTLKKEKLTFNTIWQRYGTFGILIILLAILAILKPTYFFS
                     SSTAPQILAQSSVNILIAMGEFFAILIAGIDLSVGSVVALTGMCMAKLMVAGVNPIVA
                     VLLGILLGAFLGFLNGLLVNVTGLHPFIITLGTQAIYRGVTLIISNSSAVYGFPASFT
                     RVISKSVIGIPLVAIIAVIVALILAYLTKKTKLGRNIYALGGNKESAWYSGINVNLHT
                     LIVFIISGICAGIAGVVLLGRVGSAEPAAATGFETYAIAASIIGGTSFFGGKGKIFGV
                     VMGGLIIGVINFGMNILSLSSTLQQVVMGALIIGSVALDRFVANKK"
     gene            complement(1527..>1949)
                     /locus_tag="ACZPE2_04025"
     CDS             complement(1527..>1949)
                     /locus_tag="ACZPE2_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012862472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319050.1"
                     /translation="GTIGLTNNKFEKECAEEQKKNLNIKCASVDQNITELSGGNQQKV
                     ILGKWLAADSQIIIFDEPTKGIDIGSKSEIYTLMRKLADEGKGVLVVSSELPELLAVC
                     DTIAVFREGKIVQTFPHKDATEEKIIMASTADLKERTN"
BASE COUNT          567 a          426 c          391 g          565 t
ORIGIN      
        1 gaaccgtcaa tctcgattaa aaacttataa ccgtattttt ctttaagctc cttagcttct
       61 ctgattttgc cgagcatttc ttttataaaa ggctgtccgg caaatccggg atccaccgtc
      121 ataattgtaa gcttatctat gcggtcaata tattctctga tatacgaaag cggggttgca
      181 ggattcaaaa ccacgccttt tttgcatcct aatgcctcga ttttgttcat aatacggaaa
      241 gcatcggtgt ttatagtttc agcgtgcgga caaatatagc ctgctccgtt ttcagctaga
      301 acttcaatat aatcatcggg atcggtgacc ataagatggc agtcaatagg ttttttggcg
      361 attgttgaaa acgcctttac aaagtccgga gacaaagtga tgtttttcac aaaatgtccg
      421 tccattatat cgacatgata gaggtcacat cgctcattta atatttccgt ctgattttta
      481 atatcaagaa gatccataca catgagcgac gggttaaaca taggttccat aaaaatacct
      541 cctttaagct gcacggttat tttttgttag ctacgaatcg gtcaagagca acggagccga
      601 tgataagagc gcccattaca acctgctgaa gcgtactcga aagtgaaagt atattcatac
      661 cgaagttgat aacgccgatg ataagaccgc ccattacaac tccgaagatc ttacccttgc
      721 ctccgaagaa gcttgttccg ccgataatcg aagcagctat tgcataagtc tcaaagcctg
      781 ttgccgctgc cggctctgcc gagcctacac gaccgagaag aaccacaccg gctataccgg
      841 cgcaaatacc ggaaataatg aaaactataa gggtatgaag atttacgttt atacccgaat
      901 accaagcgga ttctttgttt ccgccgagtg catagatatt acgaccaagc tttgtttttt
      961 tagtgagata cgcaaggatc aaagctacaa taaccgctat tatcgcaaca agaggaatac
     1021 ctataacaga ttttgaaata actcttgtaa aacttgccgg gaagccgtaa accgcgcttg
     1081 aatttgagat tataagcgta acgccgcgat aaattgcctg tgtgccaagc gtaatgatga
     1141 acggatgaag tcctgtaaca tttacaagga gtccgttaag gaaaccgagg aatgcaccga
     1201 gcaaaattcc gagaagaaca gcaactatcg ggtttacccc tgccaccatt aactttgcca
     1261 tacacattcc ggtcagggct accacagacc ctactgaaag atcgatgccg gcaataagaa
     1321 ttgcaaagaa ctcacccatt gcaataagta tatttaccga gctctgtgcc aaaatctgag
     1381 gtgccgtaga gcttgagaaa aaatatgttg gttttagtat ggccaagatg gcaagaagga
     1441 ttataagaat accaaacgta ccgtacctct gccagatcgt gttaaaggtt aatttttctt
     1501 tttttaatgt tttagactgt gacattttaa ttagtccttt ctttaaggtc ggcagttgaa
     1561 gccataatta ttttttcttc tgttgcgtct ttatgcggga aggtttgtac gatcttaccc
     1621 tcacggaaaa ccgctatggt atcacacacc gccaaaagtt ccggaagctc tgatgataca
     1681 acaagtacgc ccttaccctc atcggcgagc ttacgcatca aagtgtaaat ctcgctttta
     1741 gaaccaatgt cgattccctt tgtaggttca tcaaaaataa ttatttgaga atctgcggca
     1801 agccatttac cgagtatgac tttttgctgg ttgccgccgg aaagctctgt tatattttga
     1861 tcaactgaag cgcattttat attcaggttt ttcttttgct cctctgcgca ttccttttca
     1921 aatttattgt ttgtaagacc tatggtgcc
//
LOCUS       DBMDVO010000225         1946 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28201_length_1946_cov_1.319241, whole genome shotgun sequence.
ACCESSION   DBMDVO010000225 DBMDVO010000000
VERSION     DBMDVO010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28201_length_1946_cov_1.319241"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1946)
                     /gene="adhE"
                     /locus_tag="ACZPE2_04030"
                     /gene_synonym="adhC"
     CDS             complement(<1..>1946)
                     /gene="adhE"
                     /locus_tag="ACZPE2_04030"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIR1319051.1"
                     /translation="IYNAYKNTKTCGVIEEDSAFGMKKVAEPIGVVAAVIPTTNPTST
                     AIFKTLVCLKTRNGIIISPHPRAKKSTIEAAKIVLEAAVKAGAPEGIIGWIDVPSLDM
                     TNLLMQEADIILATGGPGMVKAAYSSGKPALGVGPGNTPAIIDDTADIVLAVNSIIHS
                     KTFDNGMICASEQSCIVHEKIYKKVKDEFLYRGCYFLKKDELDKVRKTIIINGALNAK
                     IVGQPAYKIAALAGVTIPETTKVLIGEVESVDISEEFAHEKLSPVLAMYKAKDFEDAL
                     SKAEHLIADGGYGHTSSVYLNAVTEKDKLAEFSERMKTCRILVNTPSSQGGIGDLYNF
                     KLAPSLTLGCGSWGGNSVSENVGVKHLINIKTVAERRENMLWFRAPQKVYFKKGCLPV
                     ALNELRDVMGKKKAFIVTDSFLYNNGYTKPVEDKLDELGIQHTCFSEVAPDPTLQCAR
                     KGTDMMRHFEPDTIIAIGGGSAMDAAKIMWVMYEHPDVDFSDMAMDFMDIRKRVYTFP
                     KMSEKAYFIAVPTSAGTGSEVTPFAIITDADTGVKWPLADYELMPNMAIVDADMMMNA
                     PKGLTSASGIDAVTHCLEAYASVMATDYTDGIAIRALQMIFEYLPRAYDNGPNDPVAR
                     EKMGNAATMAGMAFANAFLGVCHS"
BASE COUNT          499 a          465 c          452 g          530 t
ORIGIN      
        1 ggagtggcat acgccgagga aagcgttggc aaaagccata cctgccatag tagcggcatt
       61 gcccatcttt tcacgtgcta cagggtcatt ggggccgtta tcatatgctc tcggaagata
      121 ttcaaatatc atctgaagag cgcgaattgc aataccgtcg gtgtaatcgg tagccattac
      181 ggaagcataa gcttcaaggc agtgtgtaac ggcatcgata cctgaagccg aagtcagacc
      241 tttcggagca ttcatcatca tatcggcatc aactattgcc atattgggca taagctcata
      301 gtcggcaagc ggccatttaa cgccggtgtc ggcatctgtg attattgcga aaggagtaac
      361 ctccgagcct gtacctgccg aggtgggtac agctatgaaa tatgctttct cactcatctt
      421 cgggaatgta taaactctct tgcggatatc cataaagtcc atagccatat cagagaagtc
      481 aacatccgga tgctcataca ttacccacat aatttttgct gcatccattg cggaaccgcc
      541 gccgattgcg ataattgtat cgggctcaaa atgacgcatc atatcggtac ccttgcgagc
      601 gcactggaga gtcgggtcgg gagcaacctc cgagaagcag gtgtgctgaa taccaagctc
      661 gtcaagctta tcctcaacag gctttgtata accgttatta taaaggaacg agtcagttac
      721 tataaacgcc tttttcttac ccataacgtc acgaagctca ttgagagcaa cgggaaggca
      781 gccttttttg aaatatactt tctgcggagc gcggaaccaa agcatatttt cccttctttc
      841 tgcaacagtt ttgatattga tgagatgctt tacaccgaca ttctcggaaa ccgagttgcc
      901 gccccaagaa ccgcagccga gtgtaagcga cggagcaagc ttgaagttgt aaaggtcacc
      961 gataccgccc tgtgaagaag gtgtattaac aagtatacga caggttttca tcctctcgga
     1021 gaattcggca agcttatcct tttcggtaac agcattaaga tatacggatg aggtgtgacc
     1081 gtagccgccg tcggctataa gatgctcagc ctttgaaagt gcgtcctcaa agtccttagc
     1141 cttatacatg gcaagcacgg gagagagctt ttcgtgagca aattcctcgg aaatgtcaac
     1201 actctcgacc tcacctatca agacctttgt tgtttcgggg attgtaacgc ctgcgagtgc
     1261 agctatctta tatgccggct gaccgactat ctttgcgtta agcgcgccgt ttatgataat
     1321 tgtctttctt accttatcaa gctcatcctt tttaaggaaa tagcaaccgc gatataaaaa
     1381 ttcgtcctta acctttttat atattttttc gtgtacgatg cacgactgct cggaagcaca
     1441 aatcataccg ttgtcaaagg ttttcgagtg gattatggag tttacagcca aaactatgtc
     1501 ggcggtatca tcaataattg cgggggtatt gcccggaccg acgccgagag ccggtttgcc
     1561 cgacgaatat gcggctttaa ccattccggg gccgcccgta gcaagtatta tatcggcctc
     1621 ctgcataaga agatttgtca tatcaagcga cggaacgtct atccagccta ttatgccctc
     1681 cggagcaccg gctttaactg ccgcttcaag aacgattttt gcagcctcta ttgtggactt
     1741 tttcgctctc gggtgtgggc ttataataat gccgtttctg gttttaagac aaaccaaggt
     1801 cttgaaaatt gcggtagatg tagggttggt ggtggggata accgccgcaa cgacgcctat
     1861 cggctctgca acctttttca taccaaacgc gctgtcttcc tctattacac cgcaagtctt
     1921 tgtattttta tatgcattgt agatat
//
LOCUS       DBMDVO010000226         1930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28520_length_1930_cov_1.060689, whole genome shotgun sequence.
ACCESSION   DBMDVO010000226 DBMDVO010000000
VERSION     DBMDVO010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28520_length_1930_cov_1.060689"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1930)
                     /locus_tag="ACZPE2_04035"
     CDS             complement(<1..>1930)
                     /locus_tag="ACZPE2_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1319052.1"
                     /translation="EGEDKASKLSAYIEKDGKKQKLGSEEFTVAGKNSKTINLEAVIK
                     NPLKWSAETPELYTLVIELKTGKTTTYKSIRFGFKKVEIVGEKILFNGQPLMIRGVNR
                     HDFDPDHGWAVPKERYYQDLDIMKRCNINSIRTSHYPDDPFFYELCDEYGFYVMDECD
                     MESHGVRRKGVPGSNPVWTNAVVDKMQRMVLRDRNHACVFMWSLGNEAGDGTNFAKMK
                     EAALELDKTRQFHYEGDFDLTKSDVISRMYPLPDIMEKLGNREPITISLYDNVANQLA
                     ADSKPIPKEAYTKPVILCEYAHAMENSLGNFQEYMDDFEKYDNMCGGFIWDFVDQSLR
                     YKAPDGDHWLYGTDFEKSEPRRPFQLPNTTAMTGSNTYFCANGIIAADRKVHPSAYEV
                     KKVYAEMKVIEKDLEKKEFTVKNKHLFTDLSEFDLKWTVTANGKEIESGIIDNLSVPP
                     LSSKDITVPFSSDNLPNAECIITFSFLRKNKERYCEKGYEQSFDQILLKKMPEALPDN
                     SKGKLQCVQIGTTATIQGENFTVKVTDGKIVSLRFDGKEYLKTPLKPNYFRALIDNDF
                     SLTNFVPFLIPLHPYYTWRTATDTAQGSITAIDEGANGEIIIYVDWAVFSFSGVKSKY
                     IIYPDGKIEVNHEGTPKAF"
BASE COUNT          442 a          427 c          463 g          598 t
ORIGIN      
        1 aaaggccttg ggagtaccct cgtggttgac ctcgattttg ccgtcgggat agataatata
       61 tttcgattta acgcccgaga atgagaatac tgcccagtct acatatatga tgatttcacc
      121 gtttgcgccc tcatctatgg ctgttattga gccctgtgcc gtatcggttg cggttctcca
      181 tgtgtaatac ggatgaagcg gaataaggaa gggaacaaag tttgtaagtg aaaagtcgtt
      241 gtcgataagc gcgcggaagt aattcggctt taagggtgtt tttaaatatt ccttgccgtc
      301 aaagcgcagc gaaactattt tgccgtcggt aacctttacc gtgaagtttt caccctgaat
      361 tgtagctgtt gtgcctatct gaacgcattg aagtttaccc tttgaattat cgggaagagc
      421 ttcgggcatc ttctttagaa gtatctggtc aaacgactgt tcatagccct tttcgcagta
      481 acgctccttg tttttgcgaa ggaatgagaa tgtgataatg cactcggcgt tcggaagatt
      541 atcggaagag aacggcaccg tgatgtcctt tgaagagagt gggggaaccg aaagattgtc
      601 gataataccc gattcaattt ctttgccgtt tgcggttacg gtccatttaa ggtcaaattc
      661 cgaaaggtcg gtgaataagt gcttattttt aacggtaaat tctttctttt caaggtcttt
      721 ttcaataacc ttcatttcgg cgtaaacctt tttgacctca tatgccgacg ggtgaacctt
      781 tctgtcggcg gcaattatac cgtttgcaca gaagtatgtg ttactgcctg tcatagcggt
      841 cgtgttggga agctggaagg gtctgcgcgg ctctgatttt tcaaagtccg taccataaag
      901 ccaatgatct ccgtcgggag ccttgtagcg aagagactgg tctacgaagt cccaaataaa
      961 tccgccgcac atattatcgt atttctcgaa gtcatccata tattcctgga agttgccgag
     1021 gctgttttcc attgcgtgcg catattcgca gagtattacg ggctttgtgt acgcctcttt
     1081 cggaatgggc tttgaatctg cggcgagctg gtttgcaacg ttatcgtaaa ggcttatggt
     1141 gattggctcg cgattgccga gcttttccat aatgtcgggg aggggataca ttctcgatat
     1201 aacgtcgctc tttgtcaggt caaaatcgcc ctcatagtgg aactgtctcg ttttgtcaag
     1261 ctccaaagcg gcttccttca tcttagcgaa gtttgtgccg tcgccggcct cattgccgag
     1321 tgaccacata aatacgcagg cgtggtttct gtcgcgaagt accattctct gcattttgtc
     1381 cacaaccgca ttagtccata cgggattgct tccgggaacg ccctttctgc ggacgccgtg
     1441 actttccata tcgcattcgt ccataacata gaaaccgtat tcgtcgcaaa gctcatagaa
     1501 gaacggatca tcaggatagt gagaagtgcg gatagaattt atgttgcagc gtttcattat
     1561 gtcgagatcc tgataatatc tctccttcgg aaccgcccaa ccgtgatccg ggtcgaaatc
     1621 gtgacggttt acaccgcgaa tcatcagagg ctggccgtta aagagaattt tttctcctac
     1681 tatctcaacc tttttgaaac cgaaacggat tgacttgtat gttgttgttt tgccggtttt
     1741 aagctcgata acaagagtgt agagctccgg agtttcggca ctccatttga gcgggttttt
     1801 tattaccgct tcaaggttta ttgttttgct gttttttccg gcaaccgtga attcctccga
     1861 acccagcttt tgctttttgc cgtctttttc aatataagca ctgagctttg aagctttgtc
     1921 ttcgccttcg
//
LOCUS       DBMDVO010000227         1929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28538_length_1929_cov_1.114880, whole genome shotgun sequence.
ACCESSION   DBMDVO010000227 DBMDVO010000000
VERSION     DBMDVO010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28538_length_1929_cov_1.114880"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="ACZPE2_04040"
     CDS             complement(<1..120)
                     /locus_tag="ACZPE2_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319053.1"
                     /translation="MKLLVLDGNSIINRAFYGIKLLTTKDGRYTNAIYGFMNIF"
     gene            248..1210
                     /gene="dusB"
                     /locus_tag="ACZPE2_04045"
     CDS             248..1210
                     /gene="dusB"
                     /locus_tag="ACZPE2_04045"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1319054.1"
                     /translation="MNIGNLHIDGFAALAPMAGVADRAFREICRGYGAAFTESEMISA
                     KALSMGDKKSYELMHITDGERPTGIQLFGNSPEVIEKAVKSVETENPDFIDFNFGCPA
                     PKIAGNGSGSALLKTPDVIGEILKSAYSVTDKPITAKIRIGWDNETKNAVKTATTIEK
                     NGAVAITVHGRTRAQMYAPPVDTEAIREVKNAVSIPVIGNGDITDGQSAALMFEKTNC
                     DLLMVGRGALGKPWVFAQINAYMTDGRILPEPPVSERMRVMVNHVKKICEYKGEKIGI
                     KEARKHAAWYIKGIRNAAEYRQSVGLLESIEQLEEIAYKISISN"
     gene            complement(1281..>1929)
                     /locus_tag="ACZPE2_04050"
     CDS             complement(1281..>1929)
                     /locus_tag="ACZPE2_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319055.1"
                     /translation="KIEVENNVDWHEKHKLAKVLFSPNVLSREVLCDTGAGFIARETH
                     KNTSWQSARFECCHHKWFDISETDGGIAVINDSKYGLGISENTLSLSLLRATERPDPE
                     SDIGKHSFAYLIYPHSGSAVDAHINDIAFEFNIPLTRADVSCQNTFDGMFLQAMKLSE
                     DGEMVVVRLSEQNGRRGKMKFPQQVYVLNMLEDKLYLTDEIDYKPFEIITIGILR"
BASE COUNT          620 a          392 c          398 g          519 t
ORIGIN      
        1 aaatatattc ataaatccat aaatggcatt ggtatatcta ccatccttgg ttgtcagaag
       61 ctttataccg taaaaagcac ggttaataat actgttcccg tccaaaacca gcagtttcat
      121 ttgtcattct cctaataaat tttcaatata ttgtatcata aaacacatac gaatgcacta
      181 tatttttaag ttgttaatat ttttagtatg tgatatactt tataaaaaac tattgaaagc
      241 tgttattatg aatatcggaa atttacacat agatggattt gcggcattgg ctcctatggc
      301 aggcgttgcc gacagagcat tcagagaaat ttgcagaggt tacggtgccg cttttacaga
      361 gagcgaaatg ataagtgcca aagcactttc catgggcgac aaaaaatcat acgagcttat
      421 gcatataaca gacggtgaac gcccgacagg tatacaatta ttcggaaatt cgccggaagt
      481 tatagaaaaa gcggtgaaat cggttgaaac cgaaaatccg gattttattg attttaactt
      541 cggatgtccc gcgcctaaaa tagccggtaa cggctccggc tcggctcttt tgaaaacgcc
      601 tgatgtaatc ggagaaatac tgaaaagtgc ctactctgtt acagataaac caataaccgc
      661 taaaatcaga atcggttggg ataacgaaac aaaaaatgct gttaaaacgg caacaacgat
      721 tgagaaaaac ggtgcggtcg caataacagt acacggaaga acgcgcgcac aaatgtatgc
      781 tcccccagtt gatacggaag caattcgcga agtaaagaat gccgtttcaa tacctgtaat
      841 aggcaatggt gacattacag acggtcaaag tgctgcgctt atgtttgaaa aaacgaactg
      901 cgacttgctg atggtcggca gaggtgctct cggcaaaccg tgggtttttg cacaaataaa
      961 cgcttatatg acagatggca gaattttacc ggagccgccc gtttcagaac gcatgcgcgt
     1021 tatggtaaac catgtaaaaa aaatatgcga atataagggc gaaaaaatag gcataaagga
     1081 agcccgtaaa cacgccgcat ggtacataaa aggcataaga aatgccgccg aataccgcca
     1141 aagcgtagga ctccttgaga gcattgagca gcttgaagaa attgcatata aaatatcaat
     1201 atcaaattaa agcttttaca tcaaaacagc cgccataagg cggctgtttt ctcatataca
     1261 atgttgcttc taaataatta tcatcttaat atgcctatgg tgataatttc aaacggctta
     1321 taatcaattt catctgtcaa atacagttta tcttcgagca tattaaggac ataaacctgc
     1381 tgtgggaatt tcattttgcc gcgtctgccg ttctgttcgg ataaacgaac aacaaccatt
     1441 tcgccgtctt cggaaagttt catcgcctgc aagaacattc cgtcaaatgt attttggcat
     1501 gaaacatcag cccttgtcag cgggatatta aattcaaatg cgatgtcatt tatatgagcg
     1561 tcaaccgccg aaccggaatg cggataaata aggtaagcga agctgtgctt gcctatgtcg
     1621 ctttcagggt caggccgctc ggtagcgcga agcaagctaa gcgataaagt gttttccgaa
     1681 atacctaatc cgtattttga gtcgtttatc acagctatac cgccgtcagt ttccgaaata
     1741 tcaaaccatt tgtgatggca acattcaaag cgtgcacttt gccaagaagt gtttttgtga
     1801 gtttcacgcg caataaatcc cgcaccggta tcacaaagga cctcacggct taaaacattc
     1861 ggagaaaaca gtaccttagc gagcttatgt ttttcgtgcc aatcaacatt gttttcgact
     1921 tcgattttt
//
LOCUS       DBMDVO010000228         1923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28677_length_1923_cov_1.188804, whole genome shotgun sequence.
ACCESSION   DBMDVO010000228 DBMDVO010000000
VERSION     DBMDVO010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_1923_cov_1.188804"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="ACZPE2_04055"
     CDS             <1..1187
                     /locus_tag="ACZPE2_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012363.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR1319056.1"
                     /translation="TAFGDCCYQHRGFEDDAKVNPFYHREFRRGMSFTYSEDCQYLNI
                     SAPKNAKKCPVLLYIHGGSFTGGSSNEGHISGKAYAENDIVFVSVNYRLGPYGFCSHP
                     DVADENGVCGNFGLFDQAAALKWIKNNISSFGGDPDRITLLGQSAGAMSVDVLLSSPL
                     TRDMVSGAVMLSGAALQRVLSRPLSPQKMKKFWDEIIKNAGKTSLSELRDTDAKTLYY
                     AWLKACRDEGIKSTLSYTLPCEDGMLLTKDTFNIKTISAMPKILGVTTADMIPIVLKL
                     LVKKWVRNDKDSKCFVYLFARNLPGDNCGAWHSSDLPYVFATLKNGWRPFTDIDYLIS
                     EQMVAAISAFVKCGDPNCREIPTWKPGTHKVMNFCEKTRLAGWKTATLIKNTLFNKGP
                     VL"
     gene            1187..>1923
                     /locus_tag="ACZPE2_04060"
     CDS             1187..>1923
                     /locus_tag="ACZPE2_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025174.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319057.1"
                     /translation="MKFSYDFKLSEHSDSSRVYVCGDITARIDFLSGSMMRVALFRDD
                     CKMLPTFTVDPQNEFLTEGRDKLSLDGFSLFAPQVNETADGERFSLGNVEAELNTHNF
                     VLSYKKDGKLLFADRAPLAYNFQNEFGNGTYHYLTREKGEKIYGLGDKSGSVNKAGRT
                     FRIETSDSMGYDAETSDPLYKHVPFYMCENSVGCYGIYYDTSDSAVMDFGREINNYYP
                     AFKFFKSDDDCLVYYVFFGSKLEILRQ"
BASE COUNT          528 a          419 c          488 g          488 t
ORIGIN      
        1 cgaccgcttt cggcgattgt tgctaccagc accgcggttt tgaggacgat gcaaaggtta
       61 atccgtttta tcacagggaa ttcaggcgcg gtatgagctt tacatacagc gaggattgtc
      121 aatatctcaa tatcagcgcg cccaaaaacg caaaaaaatg tcccgttttg ctgtatatac
      181 acggcggttc ttttacgggc ggcagctcaa acgaggggca tatcagcggt aaggcatatg
      241 ccgaaaatga cattgttttt gtctctgtga attaccgtct cggaccgtac ggcttttgtt
      301 ctcaccccga tgtcgccgat gaaaacggag tatgcggtaa tttcggcctt tttgaccaag
      361 ctgcggcact taaatggata aaaaacaaca tttcttcctt cggcggagat ccagacagaa
      421 taaccctttt ggggcagagc gcaggagcca tgagcgtaga tgtgcttctg tcaagtccgc
      481 ttacaaggga tatggtgtcg ggtgccgtta tgttgagcgg agccgcgctt caacgagttt
      541 tgtcacgccc gctgtcgccg caaaaaatga aaaaattctg ggacgaaata atcaaaaatg
      601 ccggtaaaac aagcctttcc gagcttagag atacggacgc gaaaaccctt tactatgcat
      661 ggcttaaggc gtgccgcgac gagggaatta aaagcacact ctcttacacg cttccgtgtg
      721 aagacggaat gcttcttacg aaagatacgt ttaacataaa aacaataagc gcaatgccga
      781 aaatactcgg cgttacaacg gcagatatga taccgatagt tttaaagctg cttgtgaaaa
      841 agtgggtgcg aaacgataag gacagtaaat gctttgtcta tctttttgcg cgcaatttgc
      901 cgggcgataa ctgcggcgca tggcattcga gtgacttgcc gtatgtgttt gcaactctca
      961 aaaacggttg gcggccgttt accgatatcg actatctcat atcggagcaa atggtggcgg
     1021 caatcagcgc ttttgtaaag tgcggagacc cgaattgcag ggaaatcccg acctggaagc
     1081 ccggaacgca taaggtgatg aatttttgcg aaaagacgcg ccttgcaggc tggaagaccg
     1141 caacgcttat aaaaaataca ctcttcaata aggggccggt gctgtaatga agttttcata
     1201 tgattttaag ctgtccgagc atagcgacag cagcagagtt tacgtttgcg gcgatattac
     1261 ggcgcgaatc gacttcttga gcggcagtat gatgcgcgtc gccttattcc gtgacgattg
     1321 taaaatgctg cccaccttta ccgtagaccc gcaaaacgaa tttcttacag agggtaggga
     1381 taagctgagc cttgacggat tttcgctgtt tgctccgcaa gtaaatgaaa cagccgatgg
     1441 cgagcgcttc tctttgggaa atgtcgaagc agagcttaat acgcacaact ttgtactgag
     1501 ctataaaaag gacggcaagc ttctttttgc cgacagagca ccgcttgcct ataattttca
     1561 gaacgaattc ggaaacggta catatcatta tttaacgcgc gaaaagggcg aaaaaatata
     1621 cggcctcggc gataagagcg gcagcgtcaa taaggcgggg cgcacctttc gcatagagac
     1681 gagcgacagt atgggttatg atgcggaaac gtcggacccg ctttataagc acgtgccgtt
     1741 ttatatgtgt gaaaattccg tcggttgcta cggtatctat tacgatacgt ccgacagtgc
     1801 cgttatggat ttcggaagag aaataaataa ttattatccc gctttcaagt tcttcaaaag
     1861 cgatgacgat tgtcttgttt attatgtgtt cttcggctca aagcttgaaa ttttaaggca
     1921 gta
//
LOCUS       DBMDVO010000229         1921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28715_length_1921_cov_1.366484, whole genome shotgun sequence.
ACCESSION   DBMDVO010000229 DBMDVO010000000
VERSION     DBMDVO010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28715_length_1921_cov_1.366484"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(55..1908)
                     /gene="thrS"
                     /locus_tag="ACZPE2_04065"
     CDS             complement(55..1908)
                     /gene="thrS"
                     /locus_tag="ACZPE2_04065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1319058.1"
                     /translation="MDIAKSIGMGLYKAACLAKIDGETCDLRTPVTKDCHVEILTFDD
                     EDGKKAFWHTTSHILAQAVKRLYPSAKLAIGPSIANGFYYDFDVDFSFTPEILEKIEA
                     EMKKIVKENIPLEKFDLSADEALKLMEEKDEPYKVELIKEHAGKGEKISFYKQAEFTE
                     LCAGPHIPDTGRVKAFKLTSIAGAYWRGDEKNKMLQRIYGISFTKQSELTEYLERIEE
                     AKKRDHRKLGKELGLFALRDEAPGMPFFLPNGMILRNTLIDYWHEVHERWGYLEISTP
                     QIMKRSLWETSGHWDHYKDNMYTTVIDGEDFAIKPMNCPGSILVYELEPHSYKELPLR
                     YGELGRVHRHELSGALHGLFRVRCFTQDDAHILLAPDQIRDEVVRISQLFDEVYNLFK
                     LPYTIELSTMPEDHMGTKEEWDKATKSLSDAIESIGKEYTVNEGDGAFYGPKLDFHIE
                     DSLGRTWQCGTIQLDFQLPERFNLEYTGADGEKHCPVMIHRVVFGSVERFIGVLIEHF
                     AGAFPLWLSPVQAVVLPIADRHADRAHEIEAELKKYGIRVKVDDRSEKIGYKIREAQL
                     QKTPYMLVVGDKEVENGTVSVRHRGEGDIGSMELSSLCDRLADEVKTKAIK"
BASE COUNT          463 a          474 c          451 g          533 t
ORIGIN      
        1 gttatcacgg tcacggtaag aggtgttttt gccaaattta ctgaccccct tttattactt
       61 tatcgctttt gttttgactt cgtctgcaag gcggtcgcaa agagacgaaa gctccatgga
      121 gcctatatcg ccctcgccgc gatggcgaac ggaaacagta ccgttttcaa cttctttgtc
      181 gccgacaacg agcatatacg gcgtcttttg aagctgcgcc tcacgtattt tatatccgat
      241 tttttcggag cgatcgtcaa cctttacgcg gataccgtat tttttaagct ctgcctcgat
      301 ttcatgcgct ctgtctgcat ggcggtcggc aatcggaaga acgacggcct gtacgggtga
      361 cagccaaagc gggaacgcgc ctgcaaaatg ctctataaga acgccgataa atctctctac
      421 cgagccgaaa acaacgcggt gaatcataac ggggcagtgc ttttcgccgt cggcacccgt
      481 gtattcaaga ttaaatctct cgggaagctg gaagtcaagc tgaatagtac cgcactgcca
      541 ggttctgccg agcgaatctt caatgtggaa gtcaagcttc ggaccgtaga acgcaccgtc
      601 gccctcattt acggtatatt ccttaccgat gctttcgatt gcgtcggaaa gtgattttgt
      661 ggctttgtcc cattcctctt ttgtgcccat atggtcctcg ggcatggttg aaagctctat
      721 ggtatacggc agcttaaaga gattataaac ctcgtcaaag agctgtgata ttcttacaac
      781 ctcgtcgcgt atctggtcgg gagcgaggag tatatgagca tcgtcctgag tgaagcaacg
      841 tacgcggaaa agtccgtgaa gcgcgcccga aagctcgtgg cgatgtactc tgccgagttc
      901 accgtagcga agaggcaatt ccttgtatga atgaggctca agctcataaa caagtatgct
      961 accggggcag ttcatcggct ttatggcgaa atcctcaccg tcgataacgg ttgtatacat
     1021 attatccttg tagtgatccc agtgacccga agtctcccaa agcgaacgct tcattatctg
     1081 aggagtggat atctcaagat aaccccacct ctcgtgaacc tcgtgccaat aatcaataag
     1141 agtattgcgg agaatcatac cgttgggaag gaagaacggc attccgggag cctcgtcgcg
     1201 aagtgcaaaa agcccaagct ccttaccgag ttttctgtgg tcgcgctttt tagcctcttc
     1261 aattctttcg agatattccg taagctcgga ctgctttgtg aaagatatac catatatacg
     1321 ctgaagcatt ttattttttt catcgccgcg ccaataagcg cctgctatgg acgtgagttt
     1381 aaatgctttt acccggcctg tatcgggaat atgcggcccc gcgcaaagct cggtgaattc
     1441 cgcctgctta taaaatgaaa tcttctcgcc tttgccggca tgctctttaa taagctcaac
     1501 cttatacggc tcgtcctttt cctccataag ctttaaagcc tcgtctgcgg agaggtcgaa
     1561 cttctcaagc ggaatatttt cctttacgat tttcttcatt tcagcctcga ttttttcgag
     1621 aatttcggga gtgaaagaaa aatccacgtc gaagtcatag taaaatccgt ttgctattga
     1681 cggtcctatt gcaagcttgg cactcggata aagacgttta acagcctgtg cgagtatatg
     1741 cgaagttgta tgccaaaatg cctttttgcc gtcctcatcg tcaaaggtaa gtatttctac
     1801 atgacaatcc tttgttaccg gagtgcgaag gtcgcaagtc tcgccgtcaa tctttgcaag
     1861 gcaggcggct ttgtaaagcc ccatgccgat agactttgct atatccatta cggtcgtgcc
     1921 g
//
LOCUS       DBMDVO010000230         1919 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28745_length_1919_cov_1.538504, whole genome shotgun sequence.
ACCESSION   DBMDVO010000230 DBMDVO010000000
VERSION     DBMDVO010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28745_length_1919_cov_1.538504"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="pepT"
                     /locus_tag="ACZPE2_04070"
     CDS             <1..1127
                     /gene="pepT"
                     /locus_tag="ACZPE2_04070"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020064598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1319059.1"
                     /translation="TSNENSESIPSTEKQTVLADYLVKELKSIGVSDAYRDKYGYVYG
                     HIPATKGFEKLPKIGLISHMDTSPDVSGKDVKAKIIKFDGTNAPMIDAKYSGEDIIVT
                     DRTTLLGADDKAGVAEIIEACREICDDAELCHGKISICFTPDEEIGRGADKFDFETFD
                     ADFAYTVDGGELGGIEYENFNAAGAKITFNGVNTHPGTAKNKMKNAVLYLAEFINMLP
                     AAEAPAHTENREGFYHVSSVNGNESKATLHMIIRDHNKQSFAKRKEFITVLVEFFNRK
                     YGENTVSAEIKDSYYNMLDVIKEHIDIVERAKKAMRKSGVEPQITPIRGGTDGARLSF
                     EGLPCPNLSTGGMNFHSIYEAIPVNAMLKIVEVIKNLVSK"
     gene            complement(1179..1586)
                     /locus_tag="ACZPE2_04075"
     CDS             complement(1179..1586)
                     /locus_tag="ACZPE2_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HIR1319060.1"
                     /translation="MLILIFTEKRSFRLTVDEALSRLQKSAFRAKFHLSDEDINYIDK
                     KGLDTVKSHAEDFIKKRLAPAVIPNDGKQTPMRGHPVFKAQHACACCCRGCLNKWYHV
                     EKGRELSYDEQRRIVNLLMAWIEREYRSYKNNT"
     gene            complement(1640..>1919)
                     /locus_tag="ACZPE2_04080"
     CDS             complement(1640..>1919)
                     /locus_tag="ACZPE2_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319061.1"
                     /translation="VLSIAPGIIRIYAISFILLPFNIYSTYYFQAIMRAKISLIASVA
                     RGIVISGALILLLPVAFGGNAVWWAMPITEAVVFVYSAVMMKKVKLSR"
BASE COUNT          599 a          426 c          399 g          495 t
ORIGIN      
        1 cgacctcaaa cgaaaacagc gagagcatac cgtcaacgga aaaacaaaca gttcttgcgg
       61 attaccttgt aaaagagctg aaaagcatag gtgtaagtga cgcttaccgt gataaatacg
      121 gttatgttta cggacatata cctgcgacaa aaggatttga gaagcttccc aaaatcggat
      181 taatttctca tatggacaca tcccccgatg tcagcggaaa agacgtcaaa gccaaaataa
      241 taaaatttga cggcacaaat gcgccgatga tagatgccaa atattcgggc gaggatatta
      301 tagttaccga ccgaacaacg cttctcggcg cggatgacaa ggccggtgtt gccgaaataa
      361 tcgaggcgtg ccgcgaaatt tgcgatgacg cagagctttg ccacggcaaa atttcaattt
      421 gttttacacc cgatgaagaa ataggcagag gtgccgacaa gtttgatttt gaaacctttg
      481 acgcggattt tgcatatacc gttgacggcg gagagcttgg cggaatagaa tatgaaaact
      541 tcaacgcggc aggtgcaaaa ataaccttca acggagtcaa cacgcatccc ggcacggcta
      601 aaaacaagat gaaaaacgct gttttgtatc ttgcggaatt tataaatatg cttcctgccg
      661 ccgaagctcc cgcgcacacc gaaaatcgcg agggatttta tcacgtttca tcggttaacg
      721 gcaacgagag caaggcaact cttcatatga taatcagaga tcacaacaag caaagctttg
      781 caaagcgcaa ggaatttatt accgtacttg ttgaattttt caaccgcaaa tacggagaaa
      841 acacggtttc ggctgaaatt aaagacagct attacaatat gcttgatgtc attaaagagc
      901 atatagacat agtagaacga gcaaaaaaag caatgcgtaa atccggtgtg gagccgcaaa
      961 ttacaccgat acgcggcgga accgacggtg caaggctttc gtttgagggt ctgccctgcc
     1021 ccaacctttc aaccggtgga atgaacttcc acagtattta tgaagcaata cctgtaaatg
     1081 ctatgctcaa gattgttgag gtcattaaaa acttagtaag caaataattg aataaagaaa
     1141 aagtcccgtg aagtttactc tccttcacgg gactgctttt atgtattatt tttataactg
     1201 cggtattctc tttctatcca agccattaac agattaacta tccgcctttg ctcgtcataa
     1261 gacaattctc tgcccttttc tacatgatac cacttattta aacaccctcg acagcagcag
     1321 gcgcaggcat gctgagcctt aaacacgggg tgaccgcgca tcggcgtttg cttaccgtcg
     1381 tttggtataa ccgccggtgc aagacgtttc tttataaaat cctccgcatg gctttttacg
     1441 gtatccaagc cttttttgtc gatataattt atatcctcat cggacagatg gaatttagct
     1501 ctgaaagcag atttttgaag ccttgagagt gcttcgtcaa cagtcaaccg aaaactcctc
     1561 ttttcagtaa aaatcaagat aagcatacac ccttttgact gcttttgcaa tgactcgttt
     1621 tttgctcgac agcagaatac tatcttgaca gcttcacttt tttcatcata acagccgaat
     1681 acacaaatac aacggcttct gttataggca ttgcccacca aactgcattt ccgccgaaag
     1741 caacaggcaa taaaagtatc agagcaccgc ttattacaat acctcttgca acggatgcga
     1801 taagtgatat cttggctctc ataatagcct ggaaatagta cgttgaatag atattgaacg
     1861 gcagcagtat aaatgaaatc gcataaattc tgattattcc gggagctatt gacagcacc
//
LOCUS       DBMDVO010000231         1916 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28812_length_1916_cov_0.857300, whole genome shotgun sequence.
ACCESSION   DBMDVO010000231 DBMDVO010000000
VERSION     DBMDVO010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28812_length_1916_cov_0.857300"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="ACZPE2_04085"
     CDS             <1..1187
                     /locus_tag="ACZPE2_04085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319062.1"
                     /translation="AHKNYVKQAKYRGEITGFAAEMIEGADIVTAFNGEEDAYRHFHE
                     INEKLKKVGTTAHFYAALTNPCTRFVNAMIYAVVAVLGSILTVKGGISVGKLSSFLTY
                     CTQYTKPFNEISSVVAEFQNALASAERVFKLLDCNDEPSDEGAKELKAKNGTVEFNNV
                     SFSYSKNKPFIEGLNIRADSGKTIAIVGPTGCGKTTLINLLMRFYDVTDGEIDIDNVN
                     IASVTRDSLRSGFGMVLQDTWLFSGTIFDNIAYGKPDATREEVIEAAKAAHAHSFIKR
                     LENGYDTFISESGDNLSEGQKQLLCIARVMLTKPPMLILDEATSSIDTRTEVRIQKAF
                     ALLMEGRTTFIVAHRLSTIKNADAILVMRDGKIIERGRHDELVEQNGFYKKLYNSQFA
                     VY"
     gene            complement(1258..1884)
                     /locus_tag="ACZPE2_04090"
     CDS             complement(1258..1884)
                     /locus_tag="ACZPE2_04090"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1319063.1"
                     /translation="MNEKFVITIARGYGSGGRTIGKMLSKNLSIPFYDRDLVYMASEE
                     SGINVKLFGQNDESVKKSIFSPASDRYTGDLIPPESSAFVSHKNIFNYQAKIIKDLAV
                     KGSCIIVGRCADYILRDMPNAVKVFVWAPMSDCIKNVKVLEPSLSDKEAERKIKKIDA
                     HRSDYYRYYTCREWDSFKNYDLCINSSSAGFDKTAKYIEEFVKIKLGK"
BASE COUNT          553 a          451 c          414 g          498 t
ORIGIN      
        1 acgcgcacaa aaattacgta aagcaggcaa aataccgcgg tgaaataacc ggatttgccg
       61 ccgagatgat agagggagcc gatatagtaa cagcattcaa cggtgaagag gacgcttacc
      121 gtcattttca cgaaataaac gaaaagctta agaaggtcgg tacaaccgcg catttctatg
      181 cggcacttac caatccctgc acaagatttg ttaacgctat gatatatgcc gttgttgctg
      241 ttctcggctc gatactcacg gtaaagggcg gtatttccgt aggtaagctc tcttcttttc
      301 tcacatactg cacacagtat acaaagccgt tcaacgaaat atcaagcgtt gttgccgaat
      361 ttcagaatgc acttgcaagc gccgaacgtg tattcaagct tcttgactgc aatgatgaac
      421 cgtcggacga gggagcaaag gagcttaaag ccaaaaacgg cacggttgaa tttaacaatg
      481 tttcgttttc atacagcaaa aacaagccgt ttatcgaggg acttaatata agggctgaca
      541 gcggcaaaac aatagccata gtaggcccca caggctgcgg caagacaaca ctcataaacc
      601 ttttaatgag attctacgat gtcaccgacg gtgaaattga catagacaac gtaaatatag
      661 cctctgtcac gcgcgactcg ctgcgttccg gattcggaat ggttcttcag gacacctggc
      721 tgttttccgg cacaatattt gacaatatag cttacggaaa acccgacgcc acacgcgagg
      781 aggtaatcga ggcggcaaag gcggctcacg ctcacagctt tataaaaagg ctcgaaaacg
      841 gctatgacac ttttatctcg gagagcggag ataacctctc tgaaggtcaa aagcagcttt
      901 tgtgcatagc acgagtaatg cttacaaagc cgcctatgct cattttagac gaagccacaa
      961 gctccattga tacaagaacc gaggtcagaa tacagaaggc gtttgccctt ttgatggagg
     1021 gcagaacaac gtttatcgtt gcgcaccgcc tctccaccat aaaaaatgcc gatgcaatcc
     1081 ttgtaatgcg cgacgggaaa ataatagaac gcggacgtca cgatgagctt gtagagcaaa
     1141 acggatttta caaaaagctc tataacagcc agtttgccgt atattaaatc ccgacagaat
     1201 aataaaaagc tccgcggtta aggtcacaag ccaaaactgc ggagcttatt tttttgttta
     1261 cttgccgagt ttaattttta caaattcctc tatgtacttt gcggtcttat caaatcccgc
     1321 agacgagctg tttatacaca ggtcgtagtt tttgaaactg tcccactcgc ggcaggtata
     1381 gtagcggtaa taatcgctgc ggtgagcgtc tatctttttg attttacgct ctgcttcctt
     1441 atcgctgagc gacggctcaa gcaccttgac gttcttaatg caatcgctca tgggtgccca
     1501 aacaaacacc ttcacagcat tcggcatatc gcgaagtata tagtcggcac atctgcctac
     1561 tattatgcac gagcccttta cggcaaggtc tttaattatt ttcgcctgat aattaaagat
     1621 gtttttatgt gaaacaaaag cactgctctc cggcggaatc aaatcgcccg tatatctgtc
     1681 gcttgccgga gaaaaaatac tttttttcac tgactcatcg ttttggccga acagttttac
     1741 gtttattccg ctctcctccg acgccatata gacaaggtcg cggtcataaa agggaatcga
     1801 aaggtttttt gacagcattt tgcctattgt tctgccgccg cttccgtatc ctcttgcgat
     1861 ggtaattaca aatttttcgt tcataaactc accgaacctc tcaaaacgat atgata
//
LOCUS       DBMDVO010000232         1911 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_28920_length_1911_cov_1.278453, whole genome shotgun sequence.
ACCESSION   DBMDVO010000232 DBMDVO010000000
VERSION     DBMDVO010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28920_length_1911_cov_1.278453"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="ACZPE2_04095"
     CDS             <1..367
                     /locus_tag="ACZPE2_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1319064.1"
                     /translation="TKRIMELTDGVGCDIYMEATGNPASVETGMNCLRKQGMLFIYSV
                     FKKKAEIDLNQISEFKELRVIGGHLSPGAFPYTIDCLEKGKINAKVMVTDIFALNEID
                     EAMSAKKPDRVSIKTVLVP"
     gene            383..1351
                     /locus_tag="ACZPE2_04100"
     CDS             383..1351
                     /locus_tag="ACZPE2_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1319065.1"
                     /translation="MKIWITAPFQELYLEKLKKDNEVCYENWFDTGIVREGKELAKLL
                     NEKGTEIFVTEEDVVQKEVFEQCPTLKMLVVCRAGTSNIDLDAAKRNNVIVVNTPGRN
                     AVGVAEMVVGMMISVSRNFYKGEKMIRDGGWTEDYYFKSFGTELDGSTVGFVGFGNVA
                     KETAKPLSAFNMRILAYDPYVSAETMKEYGAEKTEDLKKLVSESDYVSNHLPVTPDTR
                     GMLDKELLSNMKETAYFINSARTATTNEEDVLNLLREKKIAGAAFDVYGKEPLSADSP
                     YLELENTELLPHIGGSTYNAIAKHSKMCFEAVRAFTENTKIDNRVV"
     gene            1367..>1911
                     /locus_tag="ACZPE2_04105"
     CDS             1367..>1911
                     /locus_tag="ACZPE2_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1319066.1"
                     /translation="MKKYAMVFDAGTGAGRCNLFDMSGRLVSYAYREWEYLKDLSSSN
                     KEAMLFDAEQFKKILFSLCRKVITQAGISADEIVAVSATSQREGMVWLDRFGKEIYAA
                     PSVDLRGEEAADILSGKEPFIKEITGLPVSGMFGLARLLWYKKFNEAVYDRIDTVMMI
                     SDWICYLLCGSKVSEPSVASSS"
BASE COUNT          605 a          356 c          494 g          456 t
ORIGIN      
        1 cacaaaaaga attatggaat taaccgacgg agtgggctgc gacatatata tggaggcgac
       61 cggaaatccc gcttcggtgg aaacgggaat gaactgtctg agaaaacagg gaatgctttt
      121 tatttacagc gtgtttaaga aaaaagcgga aatcgacctt aatcagataa gtgaatttaa
      181 agagcttcgt gtaatcggag ggcatttgag ccccggagct tttccgtaca caatagattg
      241 tctggaaaaa ggcaaaataa atgcaaaggt tatggtaacc gatatctttg ctttaaatga
      301 aattgatgag gccatgtcgg cgaaaaagcc ggacagagtt tcaattaaaa cggttttagt
      361 gccataagaa ggggtagtca caatgaaaat atggattaca gcaccgtttc aggagcttta
      421 tcttgaaaag ctgaaaaaag acaacgaggt ttgctatgaa aactggttcg atacgggaat
      481 cgtaagagag ggcaaagagc tggcaaagct tttaaacgaa aaaggaactg aaatattcgt
      541 aaccgaggag gatgttgttc aaaaggaagt ttttgaacaa tgcccgacgc tcaaaatgct
      601 tgttgtttgc agagcgggta cctcaaatat agatctcgac gccgcaaaaa ggaacaatgt
      661 tatagttgtc aacactccgg gaagaaatgc ggtaggcgtt gccgaaatgg ttgtcggaat
      721 gatgataagc gtttcaagaa atttctataa gggtgaaaaa atgatacgcg acggaggctg
      781 gaccgaggat tattatttca aatccttcgg aacggagctt gacggcagca cagtcggctt
      841 tgtgggcttc ggaaacgttg ccaaggaaac agcaaaaccc ctttcggcat tcaatatgag
      901 aattttagct tatgacccat atgtttctgc agagactatg aaggaatacg gagcggaaaa
      961 gacggaggac ctgaaaaagc tggtttcgga gagcgactac gtatcaaacc acttgccggt
     1021 aacccccgat acaagaggga tgcttgataa ggaactcctg tcaaatatga aggaaacggc
     1081 atatttcatc aattcggcaa gaaccgctac gacaaacgaa gaggacgttc ttaatctcct
     1141 ccgcgaaaag aaaatagcgg gagccgcatt tgacgtatac ggaaaagaac cgctcagcgc
     1201 ggacagcccg tatttggagc ttgaaaacac ggagcttctt ccgcatatcg gaggctctac
     1261 atacaacgct atcgcaaagc actcaaaaat gtgctttgag gccgtgaggg catttaccga
     1321 aaatacaaaa atcgataaca gagttgtata gacttaggag aataacgtga aaaagtacgc
     1381 tatggttttc gatgcaggca cgggagcagg cagatgcaat ctctttgata tgtcaggccg
     1441 tttggtttcc tatgcttaca gagaatggga atacctgaag gacctttcct caagcaacaa
     1501 agaggctatg ctttttgatg cggagcaatt taaaaaaatc ctgttttcgc tttgccgtaa
     1561 ggtaataacg caggcaggaa tatccgccga tgaaattgtt gccgtgagtg ctacctctca
     1621 aagagagggc atggtttggc ttgaccgttt cggtaaagag atatatgcag ctcccagcgt
     1681 agatttaagg ggagaggaag cggcagatat tctgtcgggt aaggagccgt tcataaaaga
     1741 aataaccggc ttgcctgttt ccggaatgtt cggactcgca agactgctgt ggtacaaaaa
     1801 atttaacgaa gctgtttatg accgaattga tacggttatg atgataagcg attggatatg
     1861 ttatttactg tgcggcagca aggtcagcga gccgtctgtt gcctcctcgt c
//
LOCUS       DBMDVO010000233         1903 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29066_length_1903_cov_1.234184, whole genome shotgun sequence.
ACCESSION   DBMDVO010000233 DBMDVO010000000
VERSION     DBMDVO010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29066_length_1903_cov_1.234184"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="ACZPE2_04110"
     CDS             <1..757
                     /locus_tag="ACZPE2_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR1319067.1"
                     /translation="KLEILKGMKKGAPLILNGDNDKLVTVREPDYKLVFFGIENPNVD
                     FRAKDIEEKNGFTSFTVEFYGASQRVTVPTVGIHNVYDALAAFAVGYEMRMEPRKIAA
                     GLKSFVPSGMRQRIKKVGGITVIEDCYNASPDSQKAALDVLSSFKSNRRIAVLGDMLE
                     LGKYSDISHENVGKYTENKNIDILFTYGAKAKHIAEGAKGFVDEIKSFDDKTELTKAL
                     LETLKDGDAVLFKASRGMKLEDVINSLYREWEE"
     gene            758..1786
                     /gene="mraY"
                     /locus_tag="ACZPE2_04115"
     CDS             758..1786
                     /gene="mraY"
                     /locus_tag="ACZPE2_04115"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1319068.1"
                     /translation="MKIWVALIFGVLLSFLIAFLLGFVIIPWLRKLHFGQTILDIGPA
                     WHKSKQGTPVMGGLMFIISIIVSFAVVLITDKLLGGHILTSGSVVIGDDMKVKIFAGL
                     LMAVAFGLIGFADDYIKVAKKRNLGLTIAQKTGAQILVMLAYLAALYMSGNTYMFIPF
                     VGNVDLKFFFWIFGIAVIYLTVNAVNFTDGVDGLCASVTSVAAISFIAAALIRGFFGV
                     SLLAGCLLGGCLGYLIWNWYPGKVMMGDTGSMFLGGMVVALAYAIDCPLIILLYGIIY
                     AVEMFSDVLQICYFKATHGKRIFKMAPIHHHFEMCGWKERKICVVFTLVQLFGGIIGT
                     ALLYYGMK"
BASE COUNT          522 a          389 c          466 g          526 t
ORIGIN      
        1 gaagcttgaa attctcaagg gtatgaaaaa gggcgcgccg cttatattaa acggcgataa
       61 cgataagctc gttaccgtaa gggagcccga ctataagctt gttttcttcg gaatcgaaaa
      121 tccgaatgtt gatttcaggg cgaaggatat tgaagaaaaa aacggtttta catccttcac
      181 ggtcgaattt tacggcgcat cacagcgcgt caccgtgcct accgtcggaa ttcacaacgt
      241 atatgacgct cttgcggcgt ttgccgtggg atatgaaatg agaatggagc cgcggaaaat
      301 agcggcaggg ctcaaaagct ttgtcccgtc cggaatgcgt cagcgtatta aaaaagtcgg
      361 cggaataacg gttatagagg attgctacaa cgcaagtccg gattcgcaaa aagcagctct
      421 ggatgtgttg tcctcgttta aatcaaaccg caggatagca gttctcggcg atatgcttga
      481 gctcggaaaa tacagcgata tttctcatga aaatgtcgga aaatataccg aaaacaaaaa
      541 tatcgacatt ctttttacat acggcgcaaa agcaaaacac attgccgagg gagcaaaagg
      601 atttgtcgat gaaataaaat catttgacga taaaaccgag cttacaaaag cgcttctcga
      661 aacgcttaaa gacggagacg cagttctttt taaggcgagt cgcggaatga agctcgagga
      721 tgttataaac agcttataca gagagtggga agagtaaatg aaaatttggg ttgcgttaat
      781 attcggcgtt ctgcttagct ttttaatagc ttttttgctc ggatttgtta taattccgtg
      841 gcttagaaag cttcacttcg ggcagactat tcttgacata ggtcctgcat ggcataaatc
      901 aaagcagggt actcccgtaa tgggcggtct tatgtttata ataagcataa tcgtatcgtt
      961 cgctgtcgtc ctgataaccg ataagctgct cggcggtcat attctgactt cgggaagcgt
     1021 tgtcataggc gacgatatga aggtcaaaat attcgcggga cttttaatgg ctgttgcttt
     1081 cggacttatc ggctttgcgg acgattatat taaggttgcc aaaaaacgta atctcggcct
     1141 taccatagcg cagaaaaccg gcgcgcagat tcttgtgatg ctggcgtatc ttgcggcgct
     1201 ctatatgtcg ggaaacacgt atatgttcat tccgttcgtc ggaaatgtcg accttaaatt
     1261 tttcttttgg atattcggaa ttgccgttat ttatctcaca gtaaatgccg taaacttcac
     1321 ggacggcgta gacggcttgt gtgcaagcgt tacctccgtg gcggcaattt catttatagc
     1381 ggcggcactt atcagaggtt tcttcggtgt aagccttctt gctggctgcc ttctcggcgg
     1441 atgtctcgga taccttattt ggaactggta tcccggaaag gttatgatgg gcgataccgg
     1501 ctcaatgttc ctcggcggta tggttgtagc cctcgcttat gccatagact gtccgcttat
     1561 aatcttgctt tacggaataa tctatgcagt cgaaatgttc tcggatgtgc ttcagatatg
     1621 ctacttcaag gcaacgcacg gcaaacgaat tttcaaaatg gcgcccatac accaccactt
     1681 tgaaatgtgc ggctggaaag agcgtaagat atgcgttgtg tttacactcg ttcagctttt
     1741 cggcggaatt ataggaaccg ctcttttgta ttacggaatg aaataatttt atttgccgat
     1801 ttcggcagat gtccgtttta atttttagga aaaggaggaa gaattatggc agggaacgcc
     1861 caaaatgcaa aaccgcgtcc tcaaaagaca aaaaaacgct cgc
//
LOCUS       DBMDVO010000234         1893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29284_length_1893_cov_1.144531, whole genome shotgun sequence.
ACCESSION   DBMDVO010000234 DBMDVO010000000
VERSION     DBMDVO010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29284_length_1893_cov_1.144531"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="metK"
                     /locus_tag="ACZPE2_04120"
     CDS             complement(<1..862)
                     /gene="metK"
                     /locus_tag="ACZPE2_04120"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1319069.1"
                     /translation="MAKHLFTSESVTEGHPDKICDNISDAVLDAILAQDPDGRVACET
                     TCITDQIMVMGEITTKAKIDIEKIARDVVCEIGYDSPEKGFDGHTCKVQIAIDKQSPD
                     IALGVDKSYELKEGYDDSYNLHGAGDQGMMFGYACDETEELMPLSISLSHKLAKQLTN
                     VRKDGTLPYLRPDGKTQVTVEYDESGAPKRVDTIVISSQHSADVSLDQIRSDIVKYVI
                     KPIVPENLLDDDTIIYVNPTGRFVTGGPAGDSGLTGRKIIVDTYGGIGRHGGGCFSGK
                     DPTKVDRSAAY"
     regulatory      complement(943..1045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1104..1724)
                     /locus_tag="ACZPE2_04125"
     CDS             complement(1104..1724)
                     /locus_tag="ACZPE2_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1319070.1"
                     /translation="MSNNKNEKKKIMSGFQIRKMTVTAILAAVSTVLMFLSFNVPFMP
                     SFIKMDFSELPALIAAFAYGPLSGVIVCLIKNLINLLSTQTGGVGELSNFILGCAFVL
                     PAGLIYKHKKTKKSAVLGALLGAVIMAAFSMVSNYYIVYPIYTKFMPMDVIIAAYSSI
                     LPSIKNLWQALFVFNLPFTFAKGLVNVLIATLIYKPLSPILKGKNI"
BASE COUNT          547 a          415 c          370 g          561 t
ORIGIN      
        1 cataagcagc agaacgatct acttttgtgg ggtcctttcc ggagaagcaa ccgccgccgt
       61 gacgaccgat accgccgtat gtgtcaacaa taatttttct tcctgtaaga ccgctgtcac
      121 ctgcagggcc gcctgtgaca aatctgcccg taggattcac ataaataatt gtatcgtcat
      181 ctaaaagatt ttcgggaaca atgggcttga taacatattt tactatgtca cttctgattt
      241 gatcaagaga gacatcagca ctgtgctgag aggaaataac aatagtgtca actcttttgg
      301 gcgcaccgct ttcatcgtat tcaacggtaa cctgtgtttt gccgtccgga cggagatagg
      361 ggagtgtacc gtcttttctg acgtttgtaa gttgctttgc aagcttatgc gaaagtgaaa
      421 ttgacagcgg cataagctct tcggtttcgt cgcaggcgta accaaacatc ataccctgat
      481 caccggcacc gtgaagatta taactgtcat catacccttc tttcagctcg tatgatttat
      541 ctacgccgag agctatatcc ggggactgct tgtcaatagc gatttgaacc ttacaggtat
      601 gtccgtcaaa gcctttttca ggtgagtcgt agcctatctc acaaacaaca tctcttgcaa
      661 ttttctcaat atcgattttt gccttcgtgg ttatttcacc cataaccatt atttggtcgg
      721 ttatacaagt tgtttcgcaa gcaactctgc cgtcaggatc ttgtgcgaga atagcatcaa
      781 gaacggcatc ggatatatta tcgcatattt tatcaggatg tccttctgta accgattctg
      841 atgtaaaaag atgtttcgcc atttttaaaa ttactccttt atgtttaaca atcaaaaaaa
      901 gaacgcccgt gctaaatccg cataaggcgt cccatagaac tcaccttatc tctcagcaca
      961 aaatgctgcg ggatttagca ccttgcatct aagtgcaggt tgccgggctt cacagggccg
     1021 atccctccgc cactctttat aaggattgtt atttatttga ttatattcta ctatttttgt
     1081 caacgtttgt caataaaatc aactcaaata ttcttccctt ttaaaatagg ggacagcggc
     1141 ttataaataa gcgttgctat cagaacattt acaagacctt tagcaaaagt gaacggtaaa
     1201 ttaaatacaa ataaagcctg ccaaagattt ttaatcgaag gcaaaataga actgtatgca
     1261 gcgattataa cgtccatcgg cataaacttg gtataaatcg gataaacaat ataatagttt
     1321 gaaaccatac tgaatgctgc cattattacg gctccgagaa gtgcgccgag aacagcactt
     1381 tttttggttt tcttgtgctt ataaatcagt cccgcgggaa gcacaaatgc acatcccaaa
     1441 ataaagttgg atagttcacc gactccgccg gtttgtgtac ttaaaagatt aatcagattt
     1501 ttaatcagac aaacaataac accgcttaac gggccatatg caaacgcagc aatcagcgcc
     1561 ggaagctctg agaaatccat tttgataaag ctcggcataa acggaacatt aaagctcaga
     1621 aacataagga cggttgaaac agccgctaaa atggctgtta cagtcatttt tctgatttga
     1681 aatcccgaca ttattttttt cttttcattt ttgttgtttg acatggtatc agacctttca
     1741 tatttttgct gaaatatgct gccaaacaaa ataaaaacct cgcagatata acctgcgagg
     1801 tgaaagtgta gcttaagtac atcttctttc atccggactg taccgtcggt tcgggagttg
     1861 cacccgatca gccgaagctt gtggactttt acc
//
LOCUS       DBMDVO010000235         1883 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29518_length_1883_cov_1.335017, whole genome shotgun sequence.
ACCESSION   DBMDVO010000235 DBMDVO010000000
VERSION     DBMDVO010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29518_length_1883_cov_1.335017"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            296..859
                     /gene="efp"
                     /locus_tag="ACZPE2_04130"
     CDS             296..859
                     /gene="efp"
                     /locus_tag="ACZPE2_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1319071.1"
                     /translation="MITAGDFRNGMTFEEDGNVMQIIEFQHVKPGKGAAFVRTKIRNV
                     ITGSVVEKSYNPTAKFPTAYVERKDMEYTYNDGDLYHFMDSETYEDVPINKAELSDNF
                     KFVKENMVCKILSYKGKVFGVEPPTFVTLEVTKTDPGFKGNTATNATKPATLETGAEI
                     KVPLFIDEGEMINVDTRTGEYMSRANS"
     gene            1004..1432
                     /locus_tag="ACZPE2_04135"
     CDS             1004..1432
                     /locus_tag="ACZPE2_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HIR1319072.1"
                     /translation="MVITMTVTEKAAYLKGVVSMLKLEESSDEAKVLNVLVDTIDEMA
                     QSIAELEEQLDLTNEQLDAVDEDLDDLEEYVYGDDDDDCCCDDDDMYEVECPSCGETV
                     YFDGAAIEDGSAECPNCGAKLDFDLDGCDCDDCGHDHDEE"
BASE COUNT          620 a          368 c          442 g          453 t
ORIGIN      
        1 gtaacattgt tacacggcta tttagccgta aataaagtaa atttaaataa aaatcggttg
       61 tatgccgtaa gggataatct attttaaaaa gtataaaacc gtaattaaga actgaagcaa
      121 aagcgaaaag caaaaaaatt taaaaaaggt ttcaaaaagg tattgcaatt acggcgggaa
      181 taatgtaaaa tataatagat agaatatggg gctgagagat aaaaaataaa aacggctttc
      241 agcggcggta tttttccgca taaacccgat aaaatatacg gaggtatttt aaaatatgat
      301 cacagcaggc gatttcagaa acggtatgac ctttgaagag gacggcaacg taatgcagat
      361 cattgagttc cagcacgtaa agcccggtaa aggtgccgct ttcgtcagaa ccaagatcag
      421 aaacgtaatt accggctcgg tagttgaaaa gtcctacaac cccacagcta agttccccac
      481 agcttatgtt gaaagaaaag acatggagta cacctataat gacggcgatc tctatcactt
      541 catggattca gagacatacg aggacgtgcc gataaacaag gcagagcttt cggacaactt
      601 caagttcgta aaggaaaata tggtttgcaa aatactttcc tacaagggca aggtattcgg
      661 cgttgagccg cccacattcg taactcttga ggttacaaag accgatcccg gcttcaaggg
      721 caacacggca acaaacgcaa caaagccggc tactcttgaa acaggtgccg agataaaggt
      781 tccgctcttt atcgacgagg gcgaaatgat taacgttgat acccgcacag gcgagtatat
      841 gtcaagagct aactcataac agagataaaa acgggggaaa taattttgcc ccatttttat
      901 agtgttcgga ttttgtgcat tgcactccga aaaaaatacg gcgttctatg tcagctacga
      961 tagggcgtcc gcgcatacaa aaatattgta gctgtgaaag gctatggtga ttactatgac
     1021 agttaccgaa aaagcggctt atctcaaagg cgtagtttca atgcttaagc tcgaggagag
     1081 ttccgacgag gctaaggttt tgaacgtact tgttgacaca atagacgaaa tggctcagtc
     1141 cattgccgag cttgaggaac agcttgacct cacaaacgag cagcttgatg ccgttgacga
     1201 ggaccttgac gatcttgagg aatatgttta cggcgatgac gatgatgact gctgctgcga
     1261 cgacgatgat atgtacgagg tagagtgccc gtcgtgcggc gagaccgtat atttcgacgg
     1321 cgctgccatt gaggatggct ctgccgagtg cccgaattgc ggcgcaaagc ttgactttga
     1381 ccttgacggc tgcgactgcg acgactgcgg tcatgaccac gacgaagaat aatctcgtca
     1441 aaataatata actgtaacgg ggcagtaaga gcgtgatgct tttactgccc tgctttttta
     1501 taccgcaaaa aaagtcggag cgaatcattt tggccgcaag tgtgaccgct gaaatcgaat
     1561 gtagatgaat tttgtaacaa aatgcacaaa cggctgtaat gaagttcgtc ggaaatttga
     1621 ttaaaatagt caaaaacccc ttgaaaaata gattttgttt tgataaaata aaaattaact
     1681 aaaaggataa gtggcaaatt gcacatttcc catgtttccc gaagggagtt aaagagcagg
     1741 atgagcacta aaacaaccaa aaagcacagc gagaacgatg ttccgcttta ccttttccat
     1801 gagggcagca attcaaatgc gtatgagtat ttcggctccc acagaaaaaa taagaatacc
     1861 gtggttttca gagtttgggc gcc
//
LOCUS       DBMDVO010000236         1882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29549_length_1882_cov_0.856261, whole genome shotgun sequence.
ACCESSION   DBMDVO010000236 DBMDVO010000000
VERSION     DBMDVO010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29549_length_1882_cov_0.856261"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            137..328
                     /locus_tag="ACZPE2_04140"
     CDS             137..328
                     /locus_tag="ACZPE2_04140"
                     /inference="COORDINATES: protein motif:HMM:NF037417.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homing endonuclease associated repeat-containing
                     protein"
                     /protein_id="HIR1319073.1"
                     /translation="MFGDDKEKWAVNELVRKSEELKRLPRKDDFDDATRSRIKAFLGP
                     WPRALEKAGLKTPGGKRKK"
     gene            351..>1882
                     /locus_tag="ACZPE2_04145"
     CDS             351..>1882
                     /locus_tag="ACZPE2_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019217.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HIR1319074.1"
                     /translation="MLKPKKTVKKCLSWLLALVMILSVIPVAVLAAPADLSISSAKEL
                     LAFSEKVNGGEDFSGKTVELTADIDLGGEDSEWTPIGSSSSAFAGTFDGNNHVISGLY
                     ISSGSNAGLFGKVNGGTIKNVTVKGSVSGSSSVAGVVGYLNAGNIIGCGNNADVSGSS
                     GVGGVVGYVGGASTVSGCYNSGNVSGTTGYIGGVSGQHWRAGKLENCYNTGKVSGPAS
                     VGGVAGGHKAASPELVNCYNAGTVEDSAGYQNNIGALIGATRGTAENCYYLSTSPFAA
                     TGNKGDVDGAAKVDSVTETMLGSAFVSGDTNPKLAWESLVSADKPVRPSFSEGTELSA
                     KLSGYIKEAVKSSKTKAGLTSADTLLGNESYMSGASSTATDWMALAMGRYGYFDSVDG
                     KYTYLIDDGTGYTDYLNAMKSYVEKTYTENGGTLHSVKATEWHRGVVTIAALGGDPAS
                     FGTYNGQPINLIADGSYNCKVKGGPGKQGINGWIWGLIALDTGMYEVPSDAAYQRELF
                     IK"
BASE COUNT          477 a          442 c          520 g          443 t
ORIGIN      
        1 gcagccgatt tatcagcttt tgatttgtgc tgtcggtgca ctctttgccc gattgcacaa
       61 gattttttcg ttggttaatt tgttgcactc tccctgcttt tcggcaggga gagatttttt
      121 gtgttcggag gattttatgt ttggcgacga caaagaaaaa tgggcagtaa atgagcttgt
      181 gcgtaaatcc gaggagctga aaaggcttcc gcgaaaagac gatttcgatg acgccacacg
      241 ctcgcgaatt aaggcatttt taggcccttg gccgcgcgct ctcgaaaaag ccggtcttaa
      301 aactcccggc gggaagcgca aaaagtaaag agttttgaaa ggagtaaggt atgttaaaac
      361 ctaaaaagac agtaaagaaa tgtctgtcat ggcttcttgc tcttgtgatg atattgtctg
      421 tgatacccgt tgcggtactt gccgcaccgg ctgatctttc aatatcgtcg gcaaaggagc
      481 tgctcgcgtt ctccgaaaag gtgaacggcg gtgaagactt ttcgggaaaa acagtagagc
      541 ttacagccga tatcgacctc ggcggagaag actccgaatg gacgccgata ggctcctcgt
      601 catcggcttt tgccggaaca ttcgacggaa acaaccacgt aatcagcgga ctttacattt
      661 cttcgggctc caatgcggga cttttcggaa aggtaaacgg cggtacgatt aagaatgtta
      721 cggttaaggg cagcgtcagc ggttcttcat cggttgcagg cgttgtcggt tacctcaatg
      781 caggcaatat aattggctgc ggaaataatg ccgatgtcag cggctcttcg ggagtcggcg
      841 gcgtagtggg ctatgtcggc ggcgcgtcta ccgtttcggg ctgctacaat tcgggtaatg
      901 tcagcggtac aacaggctat ataggcggcg taagcggtca gcattggcgc gcaggcaaac
      961 tcgaaaactg ctataacaca ggtaaggtat cgggtcctgc ctctgtcggc ggcgtagccg
     1021 gcggacacaa agcggcgtct cccgagcttg taaattgcta caatgcaggc acggttgaag
     1081 actcggcagg ctaccaaaac aatatcggcg cattgatagg tgccacaaga ggaacagcgg
     1141 agaactgcta ttatctctca acctcgcctt tcgcggcaac aggcaataaa ggcgatgttg
     1201 acggcgcggc aaaggttgac tccgtaaccg agactatgct cggttcggca tttgtttcgg
     1261 gcgacacaaa tccgaagctt gcttgggaaa gccttgtttc cgcagataag cccgttcgtc
     1321 cttcgttcag cgagggaacc gaactttcgg caaagctttc gggttacatt aaagaggcag
     1381 tcaagagctc aaaaaccaaa gcggggctca cttccgccga cactcttttg ggcaatgaaa
     1441 gctatatgtc aggcgccagc tctacggcta ccgactggat ggcacttgct atgggtcgtt
     1501 acggatattt cgattccgta gacggaaaat atacttatct tatcgacgac ggcacaggct
     1561 acaccgacta tctcaatgcc atgaagtcat acgtcgaaaa gacatacacc gaaaacggcg
     1621 gtacacttca cagcgtaaag gctaccgagt ggcacagagg cgttgttaca atagcggctc
     1681 tcggcggcga ccccgcaagc ttcggaacat ataacggtca gccgataaat cttatcgcgg
     1741 acggcagcta taactgcaag gtaaagggcg gtcccggcaa acagggtata aacggctgga
     1801 tatggggtct tattgctctt gataccggta tgtatgaggt tccgagcgac gccgcatatc
     1861 agcgtgagct tttcataaaa ga
//
LOCUS       DBMDVO010000237         1879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29602_length_1879_cov_1.501687, whole genome shotgun sequence.
ACCESSION   DBMDVO010000237 DBMDVO010000000
VERSION     DBMDVO010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29602_length_1879_cov_1.501687"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="ACZPE2_04150"
     CDS             complement(<1..1704)
                     /locus_tag="ACZPE2_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1319075.1"
                     /translation="MTKGMRKDFQREIKLNFSRFLSITLIVVLGVAFFSGLRAAKPAM
                     QASADKQYDSENLMDIRVAGTLGMTDKDVDALKKVDGVQDAEGTYEQDFLCDTGNSEA
                     VTKVMSLNDGISLVRVSEGRFPQGYNECIADRSFLDATGYKIGDTVKLTTGTDEKVSK
                     YLATDEFTIVGVGTTSMYMDSDRGSAAIGTGRINGFLIVPKQAFTMSCYTSVHITVKD
                     AMALGCYSSDYTELISKVKDRINGIADRRCDIRYSEYKNETATYINDAKLKFEEEKDT
                     AMGQLGDAYQQLLDAQAAIETGKADLASKKLEIQNAQDLINSNANTLVVSQSQIDNAK
                     ASLVEAEKKYKELESQVSDSAAEIERMQKELDDGKGSMSSDEYLQKSYNLIAAKATLQ
                     FYKTQLANTRNTIDNAKQQVAAAQTAVNNGGTALQDAQKKVNEAPAALEEAEKQIQDA
                     QIELDRKNEEYEQAKQDLADELEAAQQKLEDSEDKILNVEKPTWYVLDRETIPSYTAY
                     KSDTDGMGSIGSVFPVIFFLVAALVSLTTMTRMVEEQRTQIGVLKALGYSRGKILGKY
                     VF"
     gene            complement(1746..>1879)
                     /locus_tag="ACZPE2_04155"
     CDS             complement(1746..>1879)
                     /locus_tag="ACZPE2_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319076.1"
                     /translation="TVIVITHNSALAPMADKVIKVKNGRVDKLLLNEHPTPVEYIEW"
BASE COUNT          382 a          492 c          424 g          581 t
ORIGIN      
        1 aaacacatac ttgccgagta ttttgcctct gctgtatcca agagccttca aaacgcctat
       61 ctgcgttctc tgctcctcga ccattcgcgt catggttgtg agagatacca aagcggcaac
      121 aaggaagaat atcacgggga acaccgagcc tattgagccc ataccgtccg tatcactctt
      181 atatgccgtg taagacggaa tggtttcgcg gtcaagcaca taccatgtgg gcttttccac
      241 gtttaatatc ttatcctcgg agtcctcaag cttttgctgc gccgcttcaa gctcgtcggc
      301 aaggtcctgc tttgcctgct cgtactcctc atttttgcgg tcaagctcta tttgagcgtc
      361 ctgaatttgc ttttcggcct cttcaagagc cgccggagcc tcgttgacct ttttctgcgc
      421 gtcctgaagt gccgttccgc cgttatttac ggcagtttga gcggcagcta cctgctgctt
      481 ggcattatct attgtgtttc gcgtattggc aagctgagtt ttgtagaact gaagcgtcgc
      541 ctttgcggct atgagattat agctcttttg gagatactca tcagaggaca tggagccctt
      601 gccgtcatca agttcctttt gcattctctc aatctccgcc gcggaatccg agacctgcga
      661 ttcaagctcc ttatactttt tttcggcttc gacaagcgag gcctttgcgt tgtctatctg
      721 gctttgactt acaacaagag tattggcgtt tgagttgata aggtcctgcg cattctgtat
      781 ttcaagcttt ttgcttgcaa gatccgcctt accggtttct attgccgcct gagcgtccaa
      841 aagctgctga tacgcgtcgc cgagctgacc cattgcggtg tccttttcct cttcgaactt
      901 cagctttgca tcgtttatat atgtcgccgt ttcgttttta tattcgctgt atcttatgtc
      961 gcagcgtctg tcggctatgc cgtttattct gtcttttacc tttgaaataa gctcggtgta
     1021 atcgcttgaa tagcagccga gtgccatggc atccttaacg gttatatgaa cgcttgtata
     1081 gcaggacatc gtaaaggcct gcttcgggac tatcaaaaat ccgtttattc tgcccgtgcc
     1141 tatcgccgcg gagcctctgt cgctgtccat atacattgac gtcgtaccga cgccgacaat
     1201 cgtaaattca tcggtggcaa gatatttgct caccttttca tcggtgcctg tggtgagctt
     1261 taccgtgtcg cctattttat atcccgtagc gtcaagaaag ctgcggtctg ctatacactc
     1321 gttatagccc tggggaaaac gcccctcgct gactcttaca aggcttatgc cgtcgtttag
     1381 tgacattacc tttgtaacgg cttcggagtt gccggtatcg cagaggaaat cctgctcgta
     1441 tgtgccctcc gcatcctgca cgccgtcaac ctttttaagc gcgtcaacat ccttatcggt
     1501 cataccgaga gtccccgcga ctcttatatc cataaggttt tcactgtcgt attgcttatc
     1561 cgccgatgcc tgcatggccg gctttgcggc gcgcagtccc gaaaagaacg ctacgcccaa
     1621 aacaacgata agagttatag acaggaacct gctgaaattc agctttattt cccgctgaaa
     1681 atcttttctc attccttttg tcatttaacg tgtacctctc tctgtcaaat ataatattcg
     1741 gtattttacc actcgatata ctcgacggga gtcgggtgct cgttgagaag aagcttgtca
     1801 actctgccgt ttttgacctt gattaccttg tctgccatgg gagctagtgc ggagttgtgg
     1861 gtgattacaa taactgtaa
//
LOCUS       DBMDVO010000238         1878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29634_length_1878_cov_0.891390, whole genome shotgun sequence.
ACCESSION   DBMDVO010000238 DBMDVO010000000
VERSION     DBMDVO010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29634_length_1878_cov_0.891390"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            17..>1106
                     /gene="pdxR"
                     /locus_tag="ACZPE2_04160"
     CDS             17..>1106
                     /gene="pdxR"
                     /locus_tag="ACZPE2_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1319077.1"
                     /translation="MDSGLNKENIPLYMRVYEYYKDLITEGKLSPGARLPSIRRCEKE
                     LSVSRTTAEAACFQLAADGYVVSKPQSGYFVTDVARREKKDGTVRGKKELEPKTDYDF
                     TSLSADRESFDFTLWRRYIKSALRQDERLLSYGEAQGERELREALCKYITEHRNAVCS
                     PDNIVIGAGVQSLLHIICAVLPRSQKVFFGESEFEQGAAVFSDHGFKSCRTKEEADII
                     YVSPSHINRLGDVMPTSERLRLIRLADRENKLIIEDDYDSEFGYFNRPTPSLQGLDCG
                     RNVIYIGTFSKLLLPSIRLSFMILPDALLGEYEKKAELYNQTASKTEQIALCSFIRDG
                     NLSIRIRKIKRLYTQKMKLLTSALKEVFG"
     assembly_gap    1107..1116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1328..>1878
                     /locus_tag="ACZPE2_04165"
     CDS             1328..>1878
                     /locus_tag="ACZPE2_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319078.1"
                     /translation="MQTDFYEDRLRVFLEEFKKNTVAYSEKIEGFLYKPCEYKVGNEL
                     PVVDESFKSFEKNDRWGGKYDAHAWFYKKLIVPKELTGKNLELTVSTQLDGWDAVNPQ
                     FIAYVDGKLQQGLDVNHREIFLDNAKGEYDIFLYAYSGRNDCLLDFNVTLNAYYENAR
                     RLYYKIAVPYEVTLYHEPYKKVYV"
BASE COUNT          556 a          389 c          474 g          449 t
ORIGIN      
        1 agttatggag aatactgtgg attccggact gaataaggaa aacataccgc tgtatatgcg
       61 agtttacgaa tattataagg atttaattac cgagggcaag ctgtctccgg gcgcaaggct
      121 tccgtcaata cggcggtgtg aaaaggaact gtcggtaagc cgcactactg ctgaggcggc
      181 gtgttttcag cttgcggcgg acggatatgt tgtatcaaag ccgcagagcg ggtactttgt
      241 gacggatgtt gcgcggcgtg aaaaaaaaga cggcacggta agaggaaaaa aagagcttga
      301 gccgaaaacc gactatgatt tcacttctct ctcggcagac agggagagct ttgatttcac
      361 gctgtggcga agatacatca aaagtgcttt gaggcaggac gaaagactgc tgtcctacgg
      421 cgaggcgcag ggggaacgcg agcttaggga ggctctttgc aaatacatca cggagcacag
      481 aaatgcggtc tgctcaccgg ataacatagt cataggcgcg ggcgtgcaaa gccttttaca
      541 tataatatgc gccgtgctgc cgcgttcgca aaaggtcttt ttcggagagt cggaatttga
      601 acagggcgca gcggtgtttt ccgaccacgg ctttaaatca tgccgtacaa aagaagaggc
      661 cgatataatt tatgtgagtc cgtcgcatat aaaccgcctc ggcgacgtta tgccgacaag
      721 tgaaagacta cgccttatcc gtcttgcgga cagagaaaac aagcttataa tcgaggatga
      781 ttacgacagc gagtttggat atttcaatcg ccccacacca tccctccagg gacttgactg
      841 cggcagaaat gtaatttata tcggtacgtt ttcaaaactg cttttaccgt ctatcagact
      901 cagctttatg atacttccgg acgcgctgct cggcgaatac gaaaaaaagg cggagctgta
      961 taaccaaacc gcctcaaaga ccgaacagat agcattgtgc agctttatcc gcgacggaaa
     1021 tctgtcaata agaataagaa aaatcaaaag actgtatacc caaaaaatga aactgctgac
     1081 atcagctctg aaagaggttt tcggcgnnnn nnnnnnggta aaggagagcg gcacggggaa
     1141 aaaatatccg cagattttgc tcttgtgctc gtccgtggcg tccgaagatt ttttgccggc
     1201 tgcaaaaaga ctttacggca gtatattcgg agcataaaaa acgttagtaa aacgctgttt
     1261 gtatgttgta ttacgcgttt tactgtgata aaatatcagc aataaaaagc aataccggga
     1321 ggcaaatatg cagacagact tttatgagga caggctcagg gtgttccttg aggagttcaa
     1381 aaaaaatacc gtggcataca gtgaaaaaat cgaaggcttt ttatataagc cgtgcgaata
     1441 taaagtcggc aacgaattgc ccgttgttga tgagagcttt aagagctttg aaaaaaatga
     1501 ccgctggggc ggaaaatatg atgcacacgc atggttctat aaaaaactga ttgttccgaa
     1561 agaactgaca ggcaaaaatc ttgagcttac cgtctccact cagcttgacg gctgggacgc
     1621 ggtaaatcct cagtttatag cgtatgttga cggcaaattg cagcaagggc ttgatgtgaa
     1681 tcaccgcgaa atattccttg ataatgcaaa gggcgaatat gatatattcc tttacgccta
     1741 ttccggcaga aacgactgct tgcttgattt taacgtgact ctcaacgcgt attacgaaaa
     1801 cgcacgccgc ctctattata aaatcgccgt gccgtatgag gttacgcttt atcacgagcc
     1861 gtataaaaag gtctatgt
//
LOCUS       DBMDVO010000239         1876 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29663_length_1876_cov_1.213521, whole genome shotgun sequence.
ACCESSION   DBMDVO010000239 DBMDVO010000000
VERSION     DBMDVO010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29663_length_1876_cov_1.213521"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="ACZPE2_04170"
     CDS             complement(<1..348)
                     /locus_tag="ACZPE2_04170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1319079.1"
                     /translation="MDNELMPKYRFHSVLDVTPEDIRKMGARAIGLDIDNTIAPDGTF
                     KFLKGVEHWLDTMRKAGIPVIIISNGTVFRVGPIAKKFGLPYVCVAAKPKPKGLLRAA
                     AKLGVPIEKLAMLG"
     gene            complement(419..595)
                     /gene="rpsU"
                     /locus_tag="ACZPE2_04175"
     CDS             complement(419..595)
                     /gene="rpsU"
                     /locus_tag="ACZPE2_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1319080.1"
                     /translation="MSQVKVKENESLESALRRFKRQTSRDGVIQEVRKREHYEKPSVK
                     RKKKSEAARKRKYK"
     assembly_gap    1520..1529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          404 c          364 g          606 t
ORIGIN      
        1 gccgagcata gcgagcttct ctatcggcac gccgagcttt gcggcagctc ttaagagccc
       61 cttgggcttc ggctttgcag caacgcaaac atacggcaga ccgaattttt tagctatcgg
      121 cccgactctg aaaaccgtac cgtttgaaat tatgataacc ggtatcccgg cttttctcat
      181 tgtgtcaagc caatgctcca caccctttaa aaatttaaag gttccgtccg gagctatggt
      241 attgtcaata tcaaggccta ttgcgcgtgc gcccattttg cgtatatcct cgggcgttac
      301 gtcaagcacg ctgtgaaagc ggtatttcgg cataagttcg ttatccataa tgctccttaa
      361 atgggatctc cctgtttttt caaaagaaag ggcggacaaa accttgtccg cctctcaatt
      421 acttatactt tcttttacga gctgcttctg acttcttttt tcttttaaca gaaggctttt
      481 cgtaatgctc tcttttacga acttcctgaa ttacgccgtc acgagaggtc tgtcttttaa
      541 agcggcgaag agcgctttcc aaagactcat tttctttaac ttttacctga ctcattctga
      601 ttccctccct ttattctggg atactgtgta attatagccc attgagaatt aactgtcaag
      661 agcgatttta cgtatatcaa catttgtaat gtataatgtc tatttgtttg cacgaacagc
      721 ccctaaaaaa aggaggtcaa acaaaaaaat tgtcaatttt cctcaaaaag ttgaaaacgt
      781 taacaatttg ttcacatttt tgtgtgcggt ttgcaataat cgcttgttat tataaggctg
      841 taccaaacga gagaagaaca ttgtggtgga tgttttaaga actcgtgcag gtcataaaag
      901 ccaaaaccca ttacattaaa gttgcccggc gcagagcgcc gggtcttttt ttgtctgttt
      961 cttctcggcc tgtattattt catcgctcac gctctcggct tcgcgtctcc ttgagagcgg
     1021 acgcgttttt ttgccgcatt caccgtgccg catttcggcg aaatacttta atacttatat
     1081 aaaaagtcga aataacgcgc acaacaaaaa aacgagcaag caaaaaggac cgtttttacg
     1141 cggtcctttt tgcttgctaa gtgtcattaa gttgtataaa aagggattgg aattggtata
     1201 tgtaaacccg ctgacgcggg gatttgacgg tttaataagg cgtttgcctt atcttacttc
     1261 catcgcatag aggcgaagca ttttaccgat cgttttccac gacaacttag cgtccttaag
     1321 ctcgttgata agctgtattt cgcattcata atccttaacg atcattctta acagctcctt
     1381 tcgtttcatc ttgactgtat agtaccaccg tttgtaattt ttgtcaagac tttttgcaca
     1441 agtttttcaa attatttttg tgcaacatta agatgtagtt tatgggttat ttgtgcatat
     1501 agtcaatttt gagtgtgctn nnnnnnnnnt attcgctttt ttgcttttat gttttcgtca
     1561 atttgacaat aagatatatg ccgtatttcc cgattaaatc cgctccgttg ccgcatttcg
     1621 gacaagcggc agccgatttt gtgcggacga gcggacagac aggcgccgac gccgcacata
     1681 caatattatg attattccga caatattgcg ccgacgatat tacaaaaatt ccgaattttg
     1741 ctaaagaagc gtgtgtgcgt ttaattcgcg gcttttaccg ccgttgtaat tccaaatttt
     1801 ttcttataaa aagcacttga caaacgcaaa aaaccgattt aaaatacaga aacgaaattg
     1861 aaattgaatt tcagtt
//
LOCUS       DBMDVO010000240         1871 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29774_length_1871_cov_1.331638, whole genome shotgun sequence.
ACCESSION   DBMDVO010000240 DBMDVO010000000
VERSION     DBMDVO010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29774_length_1871_cov_1.331638"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="ACZPE2_04180"
     CDS             <1..260
                     /locus_tag="ACZPE2_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319081.1"
                     /translation="TLAKDTILISIFIIALAVKKCNISLLILAILTKMWREFLAPHQI
                     YKILDFCMDIYLRRGVLCMKMYIFDKKKKGGVLCRIILLKI"
     gene            308..715
                     /locus_tag="ACZPE2_04185"
     CDS             308..715
                     /locus_tag="ACZPE2_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1319082.1"
                     /translation="MYLRNVHDTEDAVHNTFMKLIEKQKCFESTEHEKAWLIRVSQNV
                     CKNMLRANSRRRESPTGDIAVNDSSAVSDVMIELSALPDELKVPVYLFYCDGYNSKEI
                     GSMLHISASAVRSRLQKARERLKEHLGSERSIV"
     gene            712..>1871
                     /locus_tag="ACZPE2_04190"
     CDS             712..>1871
                     /locus_tag="ACZPE2_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319083.1"
                     /translation="MSIRNEFEKIDPTEKQKKDIFRRIEESSKLKTDFRHQVIRYTSV
                     AAAAVVVVGTAAVGVHFVTKGNGIDYTVSGSSSGADSAVDASASKVNDNKSDVSDVVS
                     KADENPSHADNSSANGNNTIYPDISTLKAQVRNDKEFWAEIGKQYGPYHYEYSTVRFD
                     FINDFDDFKLCTDEAISNEIIDRVKRQWLFDNIVDFGSGYYAIKGAKDLSTSEDIEKF
                     TRENLINGEYFEADPEFFGFSDMKGFYDAASELYTGFDYDDFKSFIPYYYAEVDGKLC
                     ISKDHGLGEKGKPPHSVELGQYLIIYDNVDPSVIYCFVAVPEMIDSSSYSFSLDFYVF
                     INTENGLRCSVTKERPITTCGLVTDELEPYFAMSSERLDKKMYLEKYDDFNAL"
BASE COUNT          590 a          324 c          456 g          501 t
ORIGIN      
        1 gtacccttgc aaaagataca attttaatta gcatttttat tatagcactc gcggtaaaaa
       61 aatgcaatat ttcactgctt attttggcga tattaacaaa aatgtggcgg gaatttttag
      121 cgccacacca aatttacaaa atattagatt tttgtatgga catttatctt cgccgtggtg
      181 tattatgtat gaagatgtac attttcgaca aaaagaaaaa gggaggggtt ttatgtcgga
      241 ttattcttct gaaaatataa gagaaatata cgagcggcac tttgtcacag tgtacagagt
      301 gtgctttatg tacctcagaa atgtacacga tactgaggac gcagtacata atacttttat
      361 gaaactgatt gaaaagcaaa agtgctttga aagtaccgag catgaaaaag cgtggctgat
      421 acgggtatct cagaacgtat gcaagaatat gctaagggca aacagcaggc ggcgtgaatc
      481 accgacagga gatattgcgg tgaatgacag ttctgcggtc agtgatgtga tgatcgaact
      541 gtcggctctt cccgatgagc ttaaggtacc cgtgtatctg ttctattgtg acggctataa
      601 cagcaaagaa atcggcagta tgcttcacat aagcgcatcg gcggtgcgtt cacggctaca
      661 aaaagcgaga gagcgattaa aagagcattt aggctcggaa aggagtatag tatgagtatc
      721 agaaacgaat ttgagaaaat cgatcctacg gaaaagcaga aaaaagatat tttcagacgt
      781 atagaagaaa gcagtaagtt aaagacggat ttcagacatc aggttattcg ctacacatcg
      841 gttgcggcgg cagcggtcgt ggttgtcgga acggcggcag tcggcgtgca ttttgtgaca
      901 aagggcaacg gtattgatta caccgtcagc ggcagttcat cgggcgctga cagcgctgtc
      961 gatgcttctg caagcaaagt gaatgacaac aaatccgatg tttcggacgt ggtaagcaag
     1021 gcggacgaaa atccatcaca cgccgacaac agttccgcaa acggcaacaa tacgatatat
     1081 cccgatataa gcaccttaaa ggcgcaggtg cgtaacgaca aggaattctg ggcggaaata
     1141 ggcaagcaat acggaccgta tcattatgag tacagcacgg taaggtttga ttttataaac
     1201 gactttgacg attttaaatt atgtaccgat gaagcaataa gtaacgaaat tatcgacagg
     1261 gtcaaaaggc agtggctgtt tgataatatc gtggatttcg gctctggata ttatgcaata
     1321 aaaggtgcaa aggatttgtc aacatcggaa gatatagaga aattcacgcg ggaaaatctg
     1381 ataaacggtg aatatttcga agctgatccg gaatttttcg gtttttcgga tatgaaaggc
     1441 ttttatgatg cggcaagcga actgtacaca ggctttgatt atgatgattt caagtctttt
     1501 ataccgtatt attatgcgga agttgacggc aagctctgta tatcgaaaga tcatgggctc
     1561 ggtgaaaaag gtaaacctcc tcacagtgtt gaactcggtc agtatctgat tatatatgat
     1621 aatgttgatc cgagtgtgat atattgcttt gtcgctgttc cggagatgat tgattcgtca
     1681 agctacagtt tttcgcttga cttttacgtt ttcatcaata ccgaaaacgg actgagatgc
     1741 agtgtgacaa aagaaagacc gataacgacc tgcggattgg ttacagatga gcttgaacct
     1801 tattttgcaa tgtcatcgga gcggcttgac aagaaaatgt atttagaaaa atacgatgat
     1861 ttcaacgctc t
//
LOCUS       DBMDVO010000241         1868 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29849_length_1868_cov_1.255801, whole genome shotgun sequence.
ACCESSION   DBMDVO010000241 DBMDVO010000000
VERSION     DBMDVO010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29849_length_1868_cov_1.255801"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1868)
                     /locus_tag="ACZPE2_04195"
     CDS             complement(<1..>1868)
                     /locus_tag="ACZPE2_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014026.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319084.1"
                     /translation="HLEEAPELGLPKIMITDGPHGLRKANPDKKSSTGGIGLGNSVPA
                     TCFPPAATSSCSWDPELLEQEGEAMGEECLKEKVSTILGPGTNIKRAPVGGRNFEYFS
                     EDPLLAGECAAAVINGVQSKGVGTSLKHFAANSQEAFRMVVNEVVDERTLRETYLTAF
                     EIAVKKAQPWTVMNSYNRINGVYASENEWLQQKVLRKEWGFEGLIVTDWGASVDRIPG
                     LKAGTDLEMPCSGDLNTNRIIAAVKDGTLDEKILDERVDTVVDLIVKSKPALEKTHTY
                     DVDAHHAIAQKIAEGSMQLLKNDDGILPLKDGQKVAVIGEMAKAPRFQGAGSSVINPT
                     KLSNAFDELQKLGVDISYAQGYYKSAPSKKDKTPRKTSAELIAEAKEAASKADVAVVF
                     VGLTEEFEGEGYDREGIEIPAEHNELVAAVAEANPNTVVVIAGGSVILMPWLKNVKGL
                     LNSGLGGQAGGIAVANILTGKVNPSGKTSETYPRAFEDNPTYGNFPGGPVTSEHRESV
                     YIGYRYYDAADIDVEFPFGFGLSYTTFEYSDIKLSKDKMKDTDTVAVSFKIKNTGSVD
                     GAEIAEVYVADKESTIFRPKKELRGFKKVFLKAGEEKEVSVELSKRAFAFYNVELG"
BASE COUNT          423 a          491 c          450 g          504 t
ORIGIN      
        1 tcgccgagct ctacgttgta gaacgcgaac gctctcttgg aaagctcgac ggatacctct
       61 ttctcttcgc ctgctttaag gaataccttc ttaaatccgc gaagctcttt cttcggacgg
      121 aaaatggtgg attccttatc ggcaacgtaa acctcagcga tttcagcgcc gtctacactg
      181 cctgtattct ttatcttaaa cgaaaccgca accgtatcgg tatccttcat tttgtccttt
      241 gagagcttaa tatcgctgta ctcaaaggtg gtgtatgaaa ggccaaaacc aaacgggaat
      301 tcaacatcaa tatcggctgc gtcataatat ctgtaaccga tgtaaaccga ttctctgtgc
      361 tccgaggtaa cgggtccgcc ggggaagttg ccgtaggtcg ggttatcctc aaatgcgcgc
      421 ggataagtct cactcgtctt accggaggga ttgactttac cggtaaggat atttgcaacg
      481 gcgatgccgc ctgcctgacc gccgagaccg gaattgagaa gtcccttaac atttttaagc
      541 cacggcataa gaattaccga gccgcctgcg attacaacaa cggtgttggg gtttgcctcg
      601 gcaaccgcag caacaagttc attgtgctct gccggaattt caattccctc tctgtcgtag
      661 ccctcgccct cgaattcttc ggtaagacct acaaatacaa cggctacatc agccttagaa
      721 gcggcctctt tagcctctgc tataagctct gcacttgtct ttctgggtgt cttgtccttc
      781 ttggagggag cactcttata atagccctga gcgtaagaaa tgtctacgcc gagcttctga
      841 agctcgtcaa aagcgtttga aagcttggtg gggttgatta cggaagaacc tgcgccctgg
      901 aagcgcggtg cctttgccat ttcgcctata acggcaacct tttggccgtc cttgagagga
      961 agtatgccgt cgtcgttttt gagaagctgc attgagccct cggctatctt ctgagctatt
     1021 gcgtgatgag cgtcaacatc atatgtatgt gtcttttcaa gagccggctt tgatttgact
     1081 ataaggtcaa ctaccgtatc aactctctca tcgagtattt tttcatcaag cgtaccgtct
     1141 tttacggcag cgatgattct gtttgtgtta aggtcgcccg agcaaggcat ttcaaggtcg
     1201 gtgcctgcct taagtccggg gattctgtca acggaagcac cccaatcggt tacgataagg
     1261 ccctcaaatc cccactcttt tctgagcacc ttttgctgaa gccattcatt ttccgaagca
     1321 taaacgccgt tgattctgtt ataagaattc ataaccgtcc agggctgagc ctttttaacg
     1381 gctatttcaa atgcggtgag ataagtctcg cgaagagttc tctcatcaac tacctcgttg
     1441 actaccatac ggaaagcctc ctgagagtta gccgcgaagt gcttgagaga agtacctacg
     1501 ccctttgact gaacgccgtt tataacggct gccgcacatt cgccggcaag aagcggatcc
     1561 tccgaaaaat actcaaagtt tctgccgcct acgggagcgc gcttgatgtt tgtgccgggg
     1621 cctaaaatgg tagatacctt ttccttaagg cactcttcgc ccattgcctc gccctcttgc
     1681 tccaaaagct ccgggtccca tgagcaggac gacgtagccg caggggggaa gcaggttgcc
     1741 ggaacagagt ttccgaggcc tatgccaccc gttgagctct ttttgtccgg attagccttg
     1801 cgaaggccgt gagggccgtc ggtaatcatt atcttcggaa gtccgagctc cggagcttcc
     1861 tcaagatg
//
LOCUS       DBMDVO010000242         1868 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29852_length_1868_cov_1.182796, whole genome shotgun sequence.
ACCESSION   DBMDVO010000242 DBMDVO010000000
VERSION     DBMDVO010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29852_length_1868_cov_1.182796"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="ACZPE2_04200"
     CDS             complement(<1..233)
                     /locus_tag="ACZPE2_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR1319085.1"
                     /translation="MNICEMTALEISNAVKNKEISVKDAVDAFFAAIDEYDGKYHCYN
                     TLCKKEAYERAEEVQKGIDSGKYNGRLAGVPIG"
     gene            complement(242..529)
                     /gene="gatC"
                     /locus_tag="ACZPE2_04205"
     CDS             complement(242..529)
                     /gene="gatC"
                     /locus_tag="ACZPE2_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR1319086.1"
                     /translation="MTIDRSLVEYLEKLGRIHLSEEERSASEKDLQSILSYIDTLNEL
                     DTDGVEPASHSFPVANVMRDDTVTNGADTENILKNAPQSRDGCFVVHKTVE"
     gene            complement(560..>1868)
                     /gene="aspS"
                     /locus_tag="ACZPE2_04210"
     CDS             complement(560..>1868)
                     /gene="aspS"
                     /locus_tag="ACZPE2_04210"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1319087.1"
                     /translation="KKMESLGFPEINTPILTCSSPEGARDYIIPSRKHEGKFYALPQA
                     PQQFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMAFATQEDVFAVAEE
                     VLYDTFTKFGGGKKVSPAPFRKIPFEEAMLKYGTDKPDLRNPLEICDLTEFFSDVDFK
                     PFKGKPVRGIVAPGCGKKSKGFFEKLLEYALSIGMKGLGYLTVLPDGSFKGPIDKFLV
                     PEKKAELNSMLNLKTDDTLFFISDNIKVVNLLAGQIRTALGERLEIIDKDRFDMCFIT
                     DFPMYEKTDEGKLDFTHNPFSMPQGGMDALENQDPLTIKAYQYDIVCNGVELSSGAVR
                     NHRPDIMIKAFELAGYTADEVEKRFGALFRAFHYGAPPHAGMAPGVDRMIMLLKDEEN
                     IREVIAFPMNSNAQDLLLGAPNTVSEQQLREVHIKLRRPVPEK"
BASE COUNT          420 a          450 c          464 g          534 t
ORIGIN      
        1 atgcctatcg gtacgcctgc gagcctgccg ttatatttac cgctgtcaat tcccttttgc
       61 acttcctcgg ctctttcata agcctctttt ttgcagaggg tgttatagca gtgatatttt
      121 ccgtcgtatt cgtctattgc cgcaaaaaat gcgtcaactg cgtcctttac ggaaatttct
      181 ttatttttta cggcatttga tatttcaaga gccgtcattt cgcaaatatt catagtttac
      241 ctcactcaac cgttttatga acgacaaagc agccgtcgcg actttgcgga gcatttttca
      301 aaatgttttc cgtatcggcg ccgtttgtaa ctgtgtcgtc acgcattacg tttgcaaccg
      361 ggaaagagtg ggaagccggc tcaacaccgt ccgtgtcaag ttcattgaga gtgtcaatgt
      421 atgagagtat gctctgcaaa tccttttcag aagcggagcg ctcttcctcg ctcaggtgta
      481 ttctgccgag cttttcgagg tattctacaa ggcttctgtc tatcgtcata agatcacttt
      541 ctttcgatta gtatgtcggt tatttttcgg gcacggggcg gcgaagcttt atgtgaacct
      601 cgcgcagttg ctgctccgaa acggtattgg gcgcaccgag aagaagatct tgggcgtttg
      661 aattcatcgg gaaagctatg acctctctga tattttcctc gtcctttaag agcattatca
      721 ttctgtcaac accgggagcc ataccggcgt gaggcggtgc tccgtaatgg aatgcgcgga
      781 acagcgctcc gaaacgcttt tcgacctcat cggctgtgta gcctgcgagc tcaaatgctt
      841 ttatcataat gtcgggacga tgatttctga ccgcgcccga ggacaactca acaccattgc
      901 agactatatc gtactgataa gcttttattg tcagcggatc ctgattttcg agagcgtcca
      961 taccgccctg aggcattgaa aacggattgt gagtgaagtc gagcttgccc tcgtccgtct
     1021 tttcatacat cgggaagtct gttataaagc acatatcgaa acggtccttg tcgataattt
     1081 caagtctctc gccgagggcc gttcttatct gaccggcaag aagatttaca accttgatgt
     1141 tgtcgcttat aaagaagagc gtgtcgtcag tcttgaggtt aagcatgctg ttaagctccg
     1201 ccttcttttc gggaacgagg aatttgtcaa taggaccctt gaaggagccg tccggaagaa
     1261 cggtaagata accgagcccc ttcatgccta tcgagagagc atattcgagg agcttttcaa
     1321 agaagccctt gctctttttg ccgcagccgg gagccacgat tcctctgacg ggtttgccct
     1381 tgaagggctt aaagtcaacg tccgagaaaa actcggtgag gtcgcaaatc tcaagcggat
     1441 tgcgaagatc cggcttatcg gtgccgtatt taagcattgc ctcttcaaac ggtatttttc
     1501 tgaacggagc ggggcttacc tttttgccgc ctccgaattt tgtgaatgtg tcatagagaa
     1561 cctcttcggc tacggcgaaa acgtcctctt gtgtggcaaa ggccatttca aagtcaagct
     1621 gatagaactc tcccggggaa cggtcggccc ttgcgtcctc gtcgcggaag cagggagcta
     1681 tctggaaata tttgtcgaag cccgaagcca taagcagctg cttaaactgc tgcggagcct
     1741 gcggcagagc atagaatttg ccctcgtgct ttcttgaggg aattatatag tctcttgcgc
     1801 cctcaggaga agagcaggtg agtatgggcg tgtttatttc cgggaagccg aggctctcca
     1861 ttttcttg
//
LOCUS       DBMDVO010000243         1866 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29894_length_1866_cov_1.147309, whole genome shotgun sequence.
ACCESSION   DBMDVO010000243 DBMDVO010000000
VERSION     DBMDVO010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29894_length_1866_cov_1.147309"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="ACZPE2_04215"
     CDS             complement(<1..282)
                     /locus_tag="ACZPE2_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319088.1"
                     /translation="MTKNGLKRALSLLLSVTFVFCASFLPASAADSKKDLENKIAAAE
                     AKKKQNEQKIKDLQNKIDGAKESNGYSSEQIDALQEQLDTYQSQITELNS"
     gene            complement(275..1189)
                     /gene="ftsX"
                     /locus_tag="ACZPE2_04220"
     CDS             complement(275..1189)
                     /gene="ftsX"
                     /locus_tag="ACZPE2_04220"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1319089.1"
                     /translation="MKGTSLKYLTHEGFRNVWVNRLMSLASVTVLMACLIIMGAGIMI
                     YFNINNVVDKVQSQNVVMVYVADDASEDETTQIGTSLKGISNVESCEFVPKEVAFQEQ
                     IQSMGGDAALFEGFDEIPLPDAYKVTVKDLSQFENTVSQIKQINKVDSVRENSDLASK
                     LLSLRHAVSIVSVGLVIMLFLVALFIISNTIRITMFSRKLEISIMKAVGATNWFIRWP
                     FMIEGMILGTISGIVSLGVLWGLYAVAEKVFAQTLSLIGFSLVPFSEYWWQILLVFVA
                     IGLFTGGFGSLVSMAKYLKEQGSVVSDD"
     gene            complement(1186..>1866)
                     /locus_tag="ACZPE2_04225"
     CDS             complement(1186..>1866)
                     /locus_tag="ACZPE2_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1319090.1"
                     /translation="DNVAFAMRCIGAKEKEVRKRVPYILSLVGLASKARNLPTELSGG
                     EQQRVALARALVNNAGLIIADEPTGNIDPEMSYEIVDLLNHINANGTTVVMVTHEHNL
                     VRHFHHRVITIEQGAVVSDSEDTEALPDDYATIERDYASDASNAERAAAAAAAVQNIA
                     SSETVEDRVAKQKEDSISPFYVQPNSDADLEKFMATYGVDSDSSDDDVDYSKYLDAIE
                     KKTGGDNR"
BASE COUNT          458 a          490 c          400 g          518 t
ORIGIN      
        1 actgttaagc tccgttattt gactttggta cgtgtcaagc tgctcctgca aagcatcaat
       61 ctgttccgag gaatagccgt tcgactcttt ggcaccgtcg attttatttt gcaaatcctt
      121 tattttttgc tcattctgtt ttttctttgc ctcggcagcc gcgattttgt tttccaaatc
      181 tttcttggaa tccgccgcgg aagccggtaa aaacgacgcg caaaaaacga atgttaccga
      241 cagtaaaagg ctcaaagctc ttttgagacc gtttttagtc atcagagacc acgctccctt
      301 gctctttcag gtatttagcc atcgaaacaa gacttccgaa acctcctgta aacaatccta
      361 ttgcaacaaa tacgagaagt atctgccacc agtactcgct gaacggcacg agcgaaaagc
      421 cgattaacga gagggtctgt gcaaacactt tttcggctac tgcataaagt ccccaaagaa
      481 ctcccaggga tactatgccg gaaatcgtgc cgagtatcat accctctatc ataaacggcc
      541 aacgaatgaa ccaatttgtg gcacctaccg ccttcattat tgaaatttca agctttcttg
      601 agaacatcgt tatgcgtatt gtatttgaaa ttatgaaaag cgcaacaagg aacagcatta
      661 taacaagccc cacgctgact atcgaaaccg catggcgaag agacaaaagc ttgcttgcaa
      721 ggtcgctgtt ttctcgcacc gaatcgactt tgtttatctg ctttatctga gaaacggtgt
      781 tttcaaactg tgacaaatcc tttaccgtga ccttatatgc gtcgggaagc ggaatttcat
      841 caaagccctc aaacagagcc gcgtcgccgc ccatgctctg tatctgctcc tggaacgcaa
      901 cttccttcgg cacaaattcg cagctttcaa cattgcttat gcctttaagt gaggtaccta
      961 tctgtgttgt ttcgtcctcc gaggcatcgt cggcaacata gaccattact acattttgcg
     1021 actgcacctt gtcgactaca ttgttaatat taaaatatat cattatgccc gcgcccataa
     1081 ttataagaca tgccataagc acggttaccg atgccaatga cataaggcga ttgacccaca
     1141 cattgcggaa gccctcgtgc gtaagatatt ttaagcttgt ccctttcacc tgttgtcacc
     1201 tccggtcttt ttttcaatgg cgtcgagata ctttgagtaa tcaacgtcat cgtcggagga
     1261 atccgaatcc acgccgtatg tcgccataaa cttttcaagg tcggcatccg aattcggctg
     1321 aacataaaac gggctgatgc tgtcttcctt ttgcttggcc acacggtcct ccaccgtctc
     1381 cgaggaggca atgttctgaa ccgcagccgc ggctgccgcc gctctttccg catttgacgc
     1441 gtcggaagca taatctcttt cgatggtggc gtaatcatcg ggaagtgcct cggtgtcttc
     1501 ggagtccgaa accacggcgc cctgctctat tgtaataacc ctgtggtgga aatgacggac
     1561 gagattatgc tcgtgagtta ccattaccac ggtggttccg tttgcgttta tatggtttaa
     1621 aaggtcaact atttcgtaag acatttcggg gtcgatgttg cccgtaggct catccgcaat
     1681 tataagcccc gcattattta caagcgcacg cgcgagcgca actctctgct gctcgccgcc
     1741 cgacagctcc gtcggcagat ttctcgcctt ggaggcaagt cctacaaggc tgagtatata
     1801 aggcacgcgt tttctgactt ccttttcctt ggcaccgatg cagcgcatcg caaatgcgac
     1861 attgtc
//
LOCUS       DBMDVO010000244         1865 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_29916_length_1865_cov_1.245465, whole genome shotgun sequence.
ACCESSION   DBMDVO010000244 DBMDVO010000000
VERSION     DBMDVO010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29916_length_1865_cov_1.245465"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..>819)
                     /locus_tag="ACZPE2_04230"
     CDS             complement(105..>819)
                     /locus_tag="ACZPE2_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1319091.1"
                     /translation="VLALCVAGAAASQDGTPASGTPFQIFSRGVAGFFEMFGIPVHFA
                     TVFMTMCVSALALTSLDAVARIGRMSFQELFAVDDMKNAKPWRKVLCNPYFSTIITLL
                     FGFVLTKIGYANIWPLFGSANQLLSALVLITLCVFMKVTGRSNKMLFPPMIIMLCVTF
                     TALVQRLIAMVKAISTAAAVTIPAGETTWGKVFLNNGLQLVIAVLLIVLGLTIVINSM
                     KSYVKSKKNSEKKEEKVNG"
     assembly_gap    820..829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<830..>1865)
                     /locus_tag="ACZPE2_04235"
     CDS             complement(<830..>1865)
                     /locus_tag="ACZPE2_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1319092.1"
                     /translation="TLVIVLIAAVVLVAAYLLYGRWLAKKWGIDPKAQTPAVKYNDGK
                     DYVPTNGWTVFSHQFSSIAGAGPVTGAIQAAAFGWLPVLLWVLLGGVFFGAVTDFGAL
                     YASVKNDGKSMGVLIEKYIGKTGRKLFLLFCWLFTLLVIAAFADMVAGTFNAYTVKDG
                     VSVLADAANTNGCAGTISIMFMVFAVIFGLIQKKFNLSGWKEAVVGLLCVVASFAIGM
                     HFPLILSKDAWSYITFVYIFFAAVLPMWLLKQPRDYMTTFMFIGMIAGAVIGLLVAHP
                     TMNLPVFTGFTNEKLGTLFPILFVTVACGAVSGFHSLVSSGTSSKTIENEKDMTKVGY
                     GAMVLESLLAV"
BASE COUNT          536 a          452 c          430 g          437 t
ORIGIN      
        1 tataggagca taaaagctct cgttaaaatc aaataataaa aagcaaaaaa caaggggctg
       61 taaaacttag ttttacagtc ccctgtccgt tttaacggta ttgatcagcc gtttactttt
      121 tcttcttttt tctcgctgtt ctttttgctc ttaacatagc tcttcataga gttgataact
      181 attgtaagac cgagcactat aagcaaaaca gctatgacaa gctgaagtcc gttgtttaag
      241 aacaccttgc cccaagtggt ctcgcctgcg ggaatcgtaa ccgctgcggc tgtgcttata
      301 gcctttacca tagctataag gcgctgaacg agtgccgtaa acgtaacgca aagcattatt
      361 atcatcggcg ggaagagcat cttgttgctt ctgcccgtaa ccttcataaa tacgcagagg
      421 gttataagta cgagagcgct caaaagctgg tttgccgaac cgaacagcgg ccagatgttt
      481 gcatatccga tttttgtaag tacaaagccg aaaagaagcg taataattgt tgagaaatac
      541 ggattgcaga gaaccttgcg ccaaggcttc gcgtttttca tatcgtcaac cgcaaacagc
      601 tcctggaagc tcattctgcc tatacgcgca acggcgtcaa ggcttgtaag cgcaagtgcc
      661 gaaacgcaca ttgtcataaa taccgtagcg aagtgtacgg gaataccaaa catttcaaag
      721 aagccggcaa cgccgcggct gaatatctgg aaaggcgtgc ccgatgcagg tgtgccgtcc
      781 tgagaagccg ccgcgcctgc tacgcaaaga gcaagtactn nnnnnnnnnt actgcgagca
      841 ggctctcaag taccattgcg ccgtagccga cctttgtcat atctttttcg ttttcaatgg
      901 tctttgatga ggttccggag gatacaaggc tgtggaagcc cgaaacagca ccgcacgcaa
      961 ccgttacgaa gagtatcggg aacagtgtgc cgagcttttc gtttgtaaag cccgtaaata
     1021 cgggaagatt cattgtgggg tgagctacaa gcagtcctat aaccgcaccg gctatcatac
     1081 cgataaacat aaacgtggtc atataatcac gcggctgctt taagagccac atgggaagca
     1141 ccgccgcaaa gaagatataa acaaatgtaa tatagctcca tgcgtccttt gagagaatga
     1201 gcggaaaatg cataccgata gcaaaagaag ccactacaca aagaagaccg acaacggctt
     1261 ccttccagcc ggagagattg aatttctttt ggataagtcc gaatattacg gcaaatacca
     1321 taaacattat tgaaatggtg cctgcacagc cgtttgtatt tgcggcatcg gcaagtaccg
     1381 aaacgccgtc ctttacggta tatgcattaa atgttcctgc aaccatatcg gcaaatgccg
     1441 ctattacaag caaagtaaac agccagcaga acaaaaggaa aagcttacgc cctgttttgc
     1501 ctatgtattt ttcaataagc acgcccatgg atttaccgtc atttttaacc gaggcgtaaa
     1561 gagcgccgaa gtccgtgacc gctccgaaaa atacgccgcc gagcaacacc caaaggagta
     1621 ccggaagcca gccgaaagcc gccgcctgta tagcgccggt tacggggcct gcaccggcta
     1681 tcgaagaaaa ctgatggctg aaaacggtcc atccgtttgt cggcacatag tccttgccgt
     1741 cattgtattt tacggcagga gtctgcgcct tgggatctat tccccatttt ttcgcaagcc
     1801 agcgtccgta aagcagatac gctgccacaa ggacaaccgc cgcgataagc acaattacaa
     1861 gtgtg
//
LOCUS       DBMDVO010000245         1860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30023_length_1860_cov_1.198977, whole genome shotgun sequence.
ACCESSION   DBMDVO010000245 DBMDVO010000000
VERSION     DBMDVO010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30023_length_1860_cov_1.198977"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(139..1119)
                     /locus_tag="ACZPE2_04240"
     CDS             complement(139..1119)
                     /locus_tag="ACZPE2_04240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1319093.1"
                     /translation="MAEDKAKKLLLIVNPVSGKKMAKRYMMKMINRFDEAGYSVTACC
                     TQKNKNAYDITLSRGAGFDLIVCVGGDGTLKETVAGVMKLGKDIPLGFIPLGSTNDFA
                     KSLGIPIDVSDAVDAIINGTPMPVDMGVFGKDSFIYVAGFGNFTAISYSANQKVKNVL
                     GKNAYYLQAVGEFFKMKPFHAKVIADGEVFEGDYFYGAFSNSYSVGGMPVLHNMGVEF
                     NDGLFEMVLVDMFKNPGEIAYLANSMVRKNVKNNRLVTIRHCKNVKFIFDKPTPFTLD
                     GEYGGAFTEIDASCIHNAVRIMLHKSDWVELPNDSSAVKSEDEKEPVEAK"
     gene            complement(1140..>1860)
                     /locus_tag="ACZPE2_04245"
     CDS             complement(1140..>1860)
                     /locus_tag="ACZPE2_04245"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1319094.1"
                     /translation="CEFFCKPDTDLEWFDYWRSYCHNWLLNLGLHDDNLRLRDHAKEE
                     LSFYSKATTDIEYLFPFGWGELWGIADRTDYDLTRHQEHSGKDLSYFDQEENRKYIPY
                     VIEPSLGADRVALAFLCDAYDEEVIDEAKNDKRIVLRLHPALAPMKAAVLPLSKKLSE
                     EAGNIYAELSKHFSVDYDDAGSIGKRYRRQDEIGTPFSITYDFDSKEDGCVTVRDRDT
                     MEQTRIPVSGLVKYIEERLEF"
BASE COUNT          450 a          433 c          427 g          550 t
ORIGIN      
        1 gcaaaatttt ttacacttct attacttctt tatcgcacat cagtaaataa taaagggttg
       61 taaaaacatt tgtttttaca acccttttca gttgatgaaa cgcgactgat tttatgccgt
      121 ttccgattta atattatgtt attttgcctc aacaggctct ttttcgtcct ccgattttac
      181 agcggaggag tcattcggca gctcgaccca gtcgcttttg tgcagcatta tgcgtactgc
      241 gttatgtata cacgaagcgt ctatttcggt aaaagctccg ccgtattcgc cgtcaagcgt
      301 aaacggtgtc ggcttgtcga atataaattt gacgtttttg caatgtctta ttgtgacaag
      361 gcggttgttc ttgacatttt tacgcaccat tgaatttgca agatatgcga tttctccggg
      421 gtttttgaac atatccacaa gtaccatttc aaaaagtccg tcgttaaact caacacccat
      481 attgtgaaga accggcatac cgccgaccga gtacgaattt gaaaatgcac cgtaaaaata
      541 gtcgccctca aaaacctcgc cgtcggctat aaccttggca tgaaaaggct tcattttgaa
      601 gaattcgccc acggcctgca aatagtaagc gtttttgccg agaacgtttt tgaccttttg
      661 gttggccgaa tacgatattg cggtaaagtt gccgaagcca gcgacatata taaagctgtc
      721 ttttccgaac acacccatat caaccggcat aggagtaccg ttgattatgg catccacggc
      781 atcgctgaca tcgatgggaa taccgagcga tttggcaaaa tcgtttgtgg agccgagcgg
      841 aatgaatccg agcggaatat ccttgccgag cttcataact ccggcaacgg tttcctttaa
      901 ggttccgtca ccgccgacgc aaactatcaa gtcgaagccg gcgcctcttg agagcgtgat
      961 gtcatacgca tttttgtttt tttgcgtgca gcaggctgtg acgctgtatc cggcctcgtc
     1021 aaaacggttt atcattttca tcatataccg ttttgccatt tttttgccgg aaaccggatt
     1081 tacaataagc aaaagctttt tagctttatc ctctgccata atggttaccc ttgcgaatgt
     1141 taaaattcga gtctttcttc aatgtatttt acaaggcctg aaacaggtat tcttgtctgc
     1201 tccatagtgt ctctgtcacg aactgtaacg cagccgtcct ctttgctgtc gaagtcatag
     1261 gttattgaga acggagtacc gatttcatcc tgacggcgat agcgcttgcc tattgagccg
     1321 gcgtcatcgt aatcgactga gaagtgcttt gaaagctcgg cataaatgtt gcctgcttcc
     1381 tccgagagtt tctttgagag cggaagcacc gccgctttca tgggcgcaag agccggatga
     1441 agtcttaaaa caattcgctt gtcgttcttt gcttcgtcaa ttacctcttc gtcgtaagcg
     1501 tcgcaaagga acgcgagtgc aactctgtcc gcaccgagtg acggctctat gacgtagggg
     1561 atatattttc tgttttcctc ctggtcaaag tatgagaggt ccttgcccga atgctcctga
     1621 tgtcttgtga ggtcgtagtc ggttctgtcg gcaatgcccc aaagctcacc ccagccgaac
     1681 gggaagagat attcgatgtc tgtggttgcc tttgagtaga atgaaagctc ttcctttgcg
     1741 tggtcgcgca ggcgaagatt gtcgtcgtga agtccgaggt tgagaagcca attgtggcag
     1801 tagcttctcc aataatcaaa ccattcgagg tcggtgtcgg gcttgcagaa gaattcgcac
//
LOCUS       DBMDVO010000246         1859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30040_length_1859_cov_1.090444, whole genome shotgun sequence.
ACCESSION   DBMDVO010000246 DBMDVO010000000
VERSION     DBMDVO010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30040_length_1859_cov_1.090444"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /gene="ruvX"
                     /locus_tag="ACZPE2_04250"
     CDS             complement(<1..417)
                     /gene="ruvX"
                     /locus_tag="ACZPE2_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1319095.1"
                     /translation="MVILSVDYGDVRTGIAVCDKFGMLASPVKVIVERDSGALADEIT
                     EIATEKKAEKIVLGLPKNMDGSEGFRAEACRQLAELISERINLPIDFEDERLTTVSAH
                     NALNATNTRGKKRKAVVDAVSAVIILEDYLRKTKNQK"
     gene            complement(411..1322)
                     /locus_tag="ACZPE2_04255"
     CDS             complement(411..1322)
                     /locus_tag="ACZPE2_04255"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1319096.1"
                     /translation="MADLGVNIAGVHFKNPVITASGTFGFGQEYARLYDISRLGGISC
                     KGTTLKERPGNPVPRVCETPSGILNCVGLQNPGVDAFIKDDLPFLEKSGTVIIANIAG
                     SAEEDYVETVSRLNGTSVDMIELNISCPNVKEGGASFGTSAASVEKITSAVKKVTEKP
                     LMVKLTPNVTDITEMALAAESAGADAVSLINTLTGMIIDINTKRPLLRNNTGGMSGAA
                     VFPVALRMVWQTASKVKIPVVGLGGITKWEDAVQMLLAGASAVQVGTASFTDAFAPLK
                     IIDGINNYLDQNGYKSVSEIVGKVEPW"
     gene            complement(1322..>1859)
                     /locus_tag="ACZPE2_04260"
     CDS             complement(1322..>1859)
                     /locus_tag="ACZPE2_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1319097.1"
                     /translation="WMSERKAGDELDILGPLGNGFFPDKTKRGIFIGGGIGCPPMLGA
                     AKEYGSDAEVILGFRSQENAILTDKFKSVAKRVEITTNDGTLGAKGFVTDVLKNMVQN
                     GENAVVYACGPTPMLKSVAALCKEKNIKCYVSLEQRMACGIGACLCCAQKTRDEDGEH
                     MKHVCKNGPVFDSEEVVF"
BASE COUNT          461 a          489 c          372 g          537 t
ORIGIN      
        1 tttttgattt ttcgtttttc tcaagtaatc ttcaagaatt ataactgcag aaacagcgtc
       61 aacaactgcc ttacgctttt taccacgggt atttgtggcg ttcagcgcat tatgcgccga
      121 aacggtagtc aatctctcgt cttcaaaatc tatcggaaga ttaatccttt ccgaaataag
      181 ctctgccagt tgccgacaag cctcggcacg aaagccctct gaaccgtcca tatttttcgg
      241 aagccccaat actatttttt cagccttttt ttctgtggct atttcagtta tctcatctgc
      301 caaagcgccg ctgtcgcgct cgacaataac cttaacgggt gacgccaaca taccgaattt
      361 atcgcaaacg gcaattccgg ttctgacatc gccgtaatca accgaaagta ttaccatggt
      421 tcaacctttc cgactatctc ggacacactc ttatacccgt tttggtcgag ataattgttt
      481 ataccgtcta taattttaag cggtgcgaat gcgtctgtaa agcttgcggt accgacctga
      541 accgcactcg ctccggctaa aagcatctgt acggcgtcct cccactttgt tatacctccg
      601 agaccgacaa cgggaatttt aaccttggat gcggtttgcc aaaccatacg aagtgccacg
      661 ggaaaaaccg ccgcacctga catcccgccc gtattattgc gaagaagcgg tcttttagtg
      721 tttatatcaa taatcattcc ggtaagcgta tttataagag aaacggcatc tgcaccggca
      781 ctctctgcgg caagagccat ttcagttatg tcggttacgt taggcgtaag ttttaccatg
      841 agcggctttt cggttacttt tttgacagcc gacgttatct tttcgacgct cgcggcactt
      901 gttccgaaag aagctccgcc ctctttaaca ttaggacacg aaatattaag ctcaatcata
      961 tccacactcg taccgttaag ccttgatacg gtttcaacgt aatcttcttc tgcgcttcct
     1021 gcaatgttag cgattataac ggtacctgac ttttccaaaa aaggcaagtc atcttttata
     1081 aacgcgtcaa cacccggatt ttgaagtccg acgcagttga gtatacctga cggcgtttca
     1141 caaactcgcg gaacaggatt tcccgggcgt tctttcagcg tagtcccctt gcaagatata
     1201 cctccgaggc gcgaaatatc ataaagacgg gcatattcct gcccaaatcc gaaagttccg
     1261 ctcgctgtaa ttacggggtt tttaaaatga actccggcta tattaacacc taaatcagcc
     1321 atcagaatac gacctcctcg gaatcaaaaa cgggtccgtt tttgcaaaca tgtttcatat
     1381 gctcgccgtc ttcatcgcga gtcttttgtg cacagcaaag gcaagcacct attccgcacg
     1441 ccatacgctg ctccagcgaa acgtagcact taatgttttt ttccttacag agcgcggcaa
     1501 cgctttttaa cattggtgtg ggtccgcagg cataaactac ggcattttcg ccgttttgaa
     1561 ccatattttt aagcacatcg gtaacaaagc cttttgcgcc tagggtaccg tcgtttgttg
     1621 taatttccac ccgcttagcg acagacttaa acttatctgt aagtattgca ttttcttggc
     1681 ttctgaagcc gagaatcacc tctgcatcgc tgccgtattc ttttgcggca ccgagcatag
     1741 gggggcagcc tatacctcct ccgataaaaa ttccgcgttt tgttttatcg gggaaaaatc
     1801 cgttgccgag aggtcctaaa atatcaagct cgtcccctgc ttttctctcc gacatccat
//
LOCUS       DBMDVO010000247         1853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30158_length_1853_cov_0.954338, whole genome shotgun sequence.
ACCESSION   DBMDVO010000247 DBMDVO010000000
VERSION     DBMDVO010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30158_length_1853_cov_0.954338"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="ACZPE2_04265"
     CDS             <1..312
                     /locus_tag="ACZPE2_04265"
                     /inference="COORDINATES: protein motif:HMM:NF042527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6591 domain-containing protein"
                     /protein_id="HIR1319098.1"
                     /translation="WWFYVIIGIALISIIGAAASGKKTEKIKWNEMVLSAQLPEPAAN
                     KGKIHENTSETLDIEVENISGSQYADYVAASKEKGFTTDAKSTSYSYRAYNFDGYCLN
                     I"
     gene            329..1006
                     /locus_tag="ACZPE2_04270"
     CDS             329..1006
                     /locus_tag="ACZPE2_04270"
                     /inference="COORDINATES: protein motif:HMM:NF042527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6591 domain-containing protein"
                     /protein_id="HIR1319099.1"
                     /translation="MSIKLQKPMEMSEISWPASNAGNQLPVPESTVGKFSYEHDDDFY
                     VYIGNTSKESYNNYVKACSEKGFTVDYDKGDDYYRAKNSEGWSVSIKYEGFNIMSIDI
                     DKPSGSSTDSNVTATAAPETAKAPDETKANDTQLVNGMRKDFKEAMDSYEAFMNEYVE
                     FMKKYQSNPNDTKLLADYAKYMSKYADMCDKFDKWESQNLNAAEQAYYVDVQARVSKK
                     LLEVSNA"
     gene            complement(1397..1705)
                     /locus_tag="ACZPE2_04275"
     CDS             complement(1397..1705)
                     /locus_tag="ACZPE2_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319100.1"
                     /translation="MDKLAIEFEEELRSKMIEAKKTCGYNPTRFNQMLSRYGGVETAR
                     RLIANAQKRGNPAEGLSTLWAHRRLDLSMEASVCDPKYAPLFSEAEIRYCKEMLEKLI
                     "
BASE COUNT          623 a          344 c          385 g          501 t
ORIGIN      
        1 tggtggtttt atgtcattat cggcatagca ttaatttcga taatcggcgc agctgcaagc
       61 ggaaagaaaa ccgaaaaaat caaatggaat gaaatggttt tgagtgcaca attgcctgag
      121 cctgctgcaa ataaaggaaa aattcacgaa aatacgtctg agacgcttga tatcgaggtt
      181 gaaaatatct ccggcagcca atatgcagat tatgttgccg cttctaagga aaagggcttt
      241 acaaccgatg caaaatcgac ttcttattca tacagggcat ataattttga cggctactgt
      301 ctgaacatat aatgacagct taaaaagctt gagtattaag ctccaaaagc ctatggaaat
      361 gtcggaaata tcctggcccg caagcaacgc gggaaatcag cttcccgtgc ccgaatctac
      421 ggtcggcaaa ttttcttacg agcatgatga cgatttttat gtgtatattg gtaacacgag
      481 caaggagagc tataacaatt atgtaaaggc ttgctcggaa aagggcttca cggtggatta
      541 cgacaagggc gatgattatt acagagccaa aaacagcgag ggttggagtg tttcaattaa
      601 atatgaaggc ttcaatatta tgagcataga cattgataag ccgagcggca gctcgaccga
      661 cagcaacgtc acggcaacgg ctgcgcccga aaccgcaaaa gcccccgatg aaaccaaagc
      721 gaacgacacg cagcttgtca acggaatgcg taaggatttc aaagaggcaa tggatagcta
      781 tgaggcattt atgaacgaat atgttgaatt catgaagaaa tatcaaagca atccgaatga
      841 taccaagctg ttggcggatt atgcaaaata tatgagcaag tatgccgata tgtgcgataa
      901 gtttgataag tgggaaagcc aaaaccttaa tgcagcggaa caggcttatt atgttgacgt
      961 tcaggcgagg gtaagtaaaa aattgcttga ggtatcaaat gcataaaata acagctgttt
     1021 agcaatgctg acgtgaaata tagaaatatg ttgtttgagg gtaatatctg cttaatatga
     1081 ggttaaataa agattctgca ttcactcgcc aaaagcctat gattaaattc agcaattctt
     1141 caggtttaaa agagaacaat gctgtgaaaa ttgatgagcc taaagtgaaa tgaaatttgc
     1201 cggaaataca actgtataaa gcgaagcgaa aaatgaagca agacgatatt tagaatatga
     1261 attagttttc taaaattaat ccgagaacac ctgaaatagt tcccccaaac gccatcaaaa
     1321 tttataattg taagtcaaaa aacaaaaggt catatctgca ataagatatg acctcttgtt
     1381 tttgcgcaat taatcgtcaa atcaattttt caagcatttc tttgcaatat ctgatttcgg
     1441 cttctgaaaa aagcggtgca tacttagggt cacagacaga agcttccata gataagtcta
     1501 aacgcctgtg agcccacagt gtggaaagtc cctctgccgg atttcccctt ttttgcgcat
     1561 ttgctatcag ccgccgtgca gtttcaacac cgccgtatcg tgataacatc tgattaaagc
     1621 gtgtcggatt atatccgcac gtttttttcg cctcaatcat tttggaccgt aattcttctt
     1681 caaattctat tgccaattta tccatataat aaccgccttt tatatttttg tgaaattttt
     1741 tatacgattt tctatttgtt aaagcaaaat atccgcccac gcccaaatgg tacacagtag
     1801 gatttaatac ggatttttga acaaaatatc ataccaaaat ctaagaaaga gag
//
LOCUS       DBMDVO010000248         1846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30297_length_1846_cov_1.218911, whole genome shotgun sequence.
ACCESSION   DBMDVO010000248 DBMDVO010000000
VERSION     DBMDVO010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30297_length_1846_cov_1.218911"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>300)
                     /locus_tag="ACZPE2_04280"
     CDS             complement(<1..>300)
                     /locus_tag="ACZPE2_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1319101.1"
                     /translation="DIHDITYENTQARERTQILMDISNKVGGILVGTGDLSELALGWC
                     TYNADHMSMYGVNCSVPKTLVRHLVRFEAEKLGGEIEQILLRVLATPVSPELLPP"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..1563)
                     /locus_tag="ACZPE2_04285"
     CDS             complement(<311..1563)
                     /locus_tag="ACZPE2_04285"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1319102.1"
                     /translation="MNTGFIKVSAVSPRVTVANVEKNLQNALKTVKAESESGSSLIVF
                     PELYLTAYTCGDLFLQDALIQSALDALIKFSKETAYSDAVICIGLPLRVSGRLYNCAA
                     VLQSGAILGIVPKTYIPNYNEYYEKRWFASSEKVNCSSVIIDDEEVPFGSNLLFTLSS
                     GAVLGVEICEDLWVPVPPSSELCLNGADIIANLSASNEAVTKNDYRKNIISVQSAKCF
                     CAYVYASSGVGESSTDLVFSGACTIAENGAILSESERFAFDGSSASAYIDFEKLNSER
                     VRNGSFSDNNEILRENDFTVIPAYVNEAEYDDIARSFYPYPFVPSNESERELRCGEIL
                     SIQSAGLAKRLAHTGLKKAVIGISGGLDSTLALLVAVKAMEMLSLPNENIICVTMPGF
                     GTTDMTYNNAVELIKALNTTFKEID"
BASE COUNT          531 a          442 c          385 g          478 t
ORIGIN      
        1 caggcggtaa aagctcggga cttacgggag ttgcaagcac acgcaaaagg atctgctcta
       61 tttcaccgcc gagtttttcg gcttcaaagc gaaccaaatg tctcacaaga gttttcggta
      121 cagagcagtt cacaccgtac atactcatat ggtcggcgtt atatgtgcac cacccaagcg
      181 caagttcgct gaggtcgcct gtgccgacga gtataccgcc gaccttgttt gaaatatcca
      241 taagtatctg cgtgcgttcc ctcgcttgcg tattttcata ggtgatgtcg tgaatatcgc
      301 nnnnnnnnnn atatctattt ccttaaaagt cgtgttaagc gcttttataa gctctacggc
      361 gttattatac gtcatatcgg tagtgccgaa gcccggcatt gtgacacata taatgttttc
      421 attgggaagc gacaacatct ccatagcctt aacggctaca agaagtgcca gcgtggaatc
      481 aagtccgccg gaaataccga taacagcctt tttaagtccg gtatgcgcaa gacgcttggc
      541 aagtcccgcg ctttgaattg agagtatttc gccgcagcga agctccctct cgctttcgtt
      601 tgacggaaca aacggatagg gataaaaact tcttgcaata tcgtcatatt ccgcctcatt
      661 tacatatgcg ggaatgacgg taaaatcgtt ttcgcgcaaa atctcgttat tgtcggaaaa
      721 agagccgttt cgtacacgct cggaattcag cttttcaaag tcgatatacg ccgaggcact
      781 gctgccgtca aacgcaaagc gttcactctc cgacaaaatc gctccgtttt ccgctattgt
      841 acaagctccc gaaaacacaa ggtcggttga cgactcgcct actcccgaag aagcatatac
      901 ataagcgcaa aagcacttgg cgctctgcac ggaaattata ttttttcgat aatcattctt
      961 tgtgacagcc tcattgctcg ccgacaaatt cgctataata tcggcaccgt taaggcaaag
     1021 ctcgctgctc ggcggaacgg gaacccacaa atcctcacag atttcaactc cgagaacagc
     1081 tcccgatgac agagtaaaaa gcaaattgct gccgaacggc acctcttcgt catcaattat
     1141 aacggatgag caatttacct tttctgagga ggcaaaccac cgcttctcat agtattcatt
     1201 atagttaggt atatacgttt tcggcacaat tccgagaata gctccgctct gcaaaacagc
     1261 ggcgcaatta taaagcctgc cgctcacacg cagaggtaag cctatacata taacggcatc
     1321 gctgtatgcc gtttcttttg aaaatttaat aagcgcgtca agcgcactct gtataagcgc
     1381 gtcctgtaaa aacaaatcgc cgcaggtgta agccgtaaga taaagctccg ggaacactat
     1441 aagagagctg cccgactcgc tttcggcctt tacggtttta agggcgtttt gaagattttt
     1501 ttcgacattt gcaaccgtta cgcgcggcga aaccgccgaa acttttataa atccggtatt
     1561 catattgagt ctccgttttt tcaaaatctt tatcagtatt ttttcaaata taataatatt
     1621 ataacacaag aattatatac cgaaaagata tatttgtcca taatcacgtt aataacgcaa
     1681 gccggttgac aacggtgtta aaattaaaac tataattata agaaggcgac tgataatatg
     1741 agaaaattca gcaaatcaca caagctcgac cacgtttgct acgatatacg aggcccggtc
     1801 cttgaaaaag cgagcgaaat ggaaagcgcc ggcacaaaaa tactga
//
LOCUS       DBMDVO010000249         1844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30343_length_1844_cov_1.104991, whole genome shotgun sequence.
ACCESSION   DBMDVO010000249 DBMDVO010000000
VERSION     DBMDVO010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30343_length_1844_cov_1.104991"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="ACZPE2_04290"
     CDS             complement(<1..409)
                     /locus_tag="ACZPE2_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HIR1319103.1"
                     /translation="MKHLRKIVSILALSAALLCVLCSVAFAASNNDSAFAVSESGAAG
                     DIINVDVYLSGKDIGSVYIDLEYDNTALEFVSARTFVAGLRFNYNSSTVLSGGNIVRF
                     TGSVDSYDALNVNGAVMRVELKILKSDVRPQIKI"
     gene            545..>1844
                     /locus_tag="ACZPE2_04295"
     CDS             545..>1844
                     /locus_tag="ACZPE2_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1319104.1"
                     /translation="MRNQPITAVIVGAGHRAFVYSKLALTDPDKLKIVGVADPDKIRQ
                     KKAMEMFGFPKENCFDSALELSRVPKFADAVINGTMDEQHVETSIPLLKKGYDMLLEK
                     PFATNEKEMRELVSCVKENGNKVMICHVLRYTPFYLSIKERVASGEIGDIINIQTCEH
                     VSYHHLSTSYVRGKWANSDVCHTSMLLAKCCHDIDLMMWMMSDTQPSMISSFGSKFQF
                     KPENAPKEAGTKCLVDCPLVDTCRYSAKRLYLDQPKRWAFYIWDKLEGIENPTDEDRI
                     NLLKGDSNYGRCIYKCDNNVVDHQSVLVNFKNGATGTHNMVGGSAGPMRRIHIIGTKG
                     EIYGDFEESKFTVAKIHPTKTDEKTVEVVDLNVNGDMVGAYGGHGGGDEKLAADFVEF
                     LRGGKPSLACTSIFDSVAGHLCVYLADKSREENGMPQKVNL"
BASE COUNT          592 a          386 c          387 g          479 t
ORIGIN      
        1 atattttaat ttgcgggcgc acatcggatt ttaatatttt aagctcaact ctcataaccg
       61 caccgtttac attaagggcg tcataactgt cgacagagcc ggtaaaccta acgatgttac
      121 caccggaaag cacagttgat gaattataat taaacctgag gcccgcaaca aatgttctcg
      181 ccgaaacaaa ttccagagca gtattgtcat attcaaggtc aatataaaca gaaccgatat
      241 ctttaccgct taaatacaca tcaacattta ttatatcgcc ggcggctcca ctctcgctta
      301 ccgcaaatgc ggaatcattg tttgaagccg caaaagctac cgaacaaagt acacataaaa
      361 gtgcggctga caaagccaat attgaaacta tttttcgcaa atgcttcacg tcgtcaactc
      421 ctttgtgttt tttatataat aacatacttt tctcacagaa acaattttat ttttcacatt
      481 atgactttat tttacaaaaa gattgatgta taatccattt aaaatattta aggaggtctg
      541 tttcgtgcgt aatcagccta tcacagccgt tattgtggga gccggtcaca gggcttttgt
      601 atattcaaag cttgccctta ccgatccgga caaattaaaa attgtcggcg ttgccgaccc
      661 ggacaaaatt cgacaaaaaa aggcaatgga aatgttcggt ttcccgaaag agaactgctt
      721 tgactcggca cttgagctct ccagagttcc taaatttgca gacgccgtaa tcaacggtac
      781 aatggatgaa cagcatgttg aaacatctat tcccctgctg aaaaagggat atgatatgct
      841 tctcgaaaag ccctttgcaa caaatgaaaa agagatgcgc gagcttgtgt catgtgtaaa
      901 agaaaacggc aacaaagtta tgatatgcca cgtactgaga tatacgccgt tttatctgtc
      961 aattaaagaa agagtagctt caggagaaat cggcgacatt ataaacatac aaacctgcga
     1021 gcacgtgagc tatcatcact tgtcaacatc gtatgtaaga ggaaaatggg ctaactccga
     1081 tgtttgccac acatctatgc tacttgcaaa atgctgtcac gatatagacc tgatgatgtg
     1141 gatgatgtcc gatacacagc cgagtatgat atcaagtttc ggcagcaaat tccaatttaa
     1201 gccggaaaat gcgccaaagg aagccggcac aaaatgtctt gtagactgcc cgcttgttga
     1261 cacatgccga tattcagcaa aaagactcta ccttgaccaa cctaaacgtt gggcatttta
     1321 tatttgggat aaacttgagg gaattgagaa ccccacagat gaagacagaa taaatctgct
     1381 taaaggcgac agcaactatg gcagatgcat ttacaaatgc gacaacaatg ttgtggacca
     1441 ccagtcggtg ctcgtgaact tcaaaaacgg agcaaccggt acgcataata tggtcggcgg
     1501 ttcggcagga ccgatgagaa gaatacatat tataggcaca aaaggcgaaa tctacggtga
     1561 ttttgaagaa tctaaattca ctgtggctaa aattcatcct actaaaacgg atgaaaaaac
     1621 ggtcgaagtt gttgatctaa atgtaaacgg tgatatggta ggcgcttacg gaggtcacgg
     1681 cggcggcgac gaaaagcttg cggccgattt cgtcgagttt ttaagaggag gtaaaccctc
     1741 tctcgcctgc acctctatat ttgattcggt ggcaggtcat ctgtgcgttt atctcgcaga
     1801 taaatcacgc gaagaaaacg gtatgccgca aaaagtaaat ttgt
//
LOCUS       DBMDVO010000250         1842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30376_length_1842_cov_1.265365, whole genome shotgun sequence.
ACCESSION   DBMDVO010000250 DBMDVO010000000
VERSION     DBMDVO010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30376_length_1842_cov_1.265365"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..807
                     /locus_tag="ACZPE2_04300"
     CDS             145..807
                     /locus_tag="ACZPE2_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012415.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR1319105.1"
                     /translation="MCLALASCAKENSGGTEPSTQDVSTSAAQETEVLSDFETETETE
                     IEKQSTAEVNATPTAADPQKTKVTQKSVKSTQEERKTRTETSPKTTAVPEPTVVKGFE
                     REMLELCNQQRRANGKPDIALNNTMCENAAVRAEEISRKNCFSHTRPDGTMCFTAVTV
                     NYMTCGENIAYGTRSASATVTAWMNSPGHKANILSEDFTEAGFGCYEVSGVRYWVQLF
                     IG"
     gene            complement(851..1549)
                     /gene="sleB"
                     /locus_tag="ACZPE2_04305"
     CDS             complement(851..1549)
                     /gene="sleB"
                     /locus_tag="ACZPE2_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HIR1319106.1"
                     /translation="MKMNSPGAKFKKMLAVCLAAVLLFAVGSIAVYKQSASHSTEVLS
                     KTGSRGEEVRQIQTKLKSKGIYSGSVDGIYGTLTKDAVKKFQKSAGLTADGIAGPKTL
                     SALGIGSASSGQYSSSDIYLLAKVIAAEARGEPYIGQVAVGAVVLNRVQHASFPDSIA
                     GVVYQPGAFSCVNDSNWSTEPTAQSRKAAQDAINGWDPSGGAIYYYNPAKTSNKWIRT
                     RPVIQTIGAHVFCK"
BASE COUNT          508 a          397 c          441 g          496 t
ORIGIN      
        1 ccatagcagg ctgatcggca ttacagtata tattatgcta aacgggaagt gtttttacac
       61 ttcccgtttt ttgttgtata aaatattgta aagtggtaaa atcaaagcgg tgataaaaat
      121 aaaaaaatta taacggtaat tgttttgtgc cttgcgcttg cgtcatgcgc aaaagaaaac
      181 agcggcggca cagagccttc gacacaagat gtaagtacga gtgcagcaca ggaaacggaa
      241 gtgctgtcgg attttgaaac cgagacggaa acagaaattg agaagcagtc aacggcagag
      301 gttaatgcta cgccgacggc cgccgaccct caaaaaacga aggtaacgca aaagtccgta
      361 aagtctacgc aagaggaaag aaaaactcgg acagagactt cgcctaagac gacagccgtt
      421 cctgagccga ctgtcgtaaa ggggtttgaa cgcgagatgc tcgagttgtg caatcagcag
      481 cgtcgtgcga acggaaaacc cgatattgct cttaacaata ctatgtgtga aaatgctgcg
      541 gtaagagccg aggaaatttc aaggaaaaac tgcttttcac atacaagacc cgacggcaca
      601 atgtgcttta ccgcggttac ggtcaattat atgacatgcg gtgaaaacat tgcttacggt
      661 acgcggtctg cttcggcgac ggtgactgcg tggatgaatt ctcccgggca taaagcgaat
      721 attctctcgg aggattttac ggaggccggc ttcggctgct atgaggtgag cggagtaagg
      781 tattgggttc aattatttat aggataggct atgagctgct ttttgtacgt gcaaaaagca
      841 gctcgcgtgc ttatttgcaa aatacgtgtg cgcctatggt ctgtataacg ggtcttgtgc
      901 gtatccactt gtttgaagtc tttgccggat tatagtagta tatggcaccg cccgacggat
      961 cccagccgtt tattgcgtcc tgagccgcct ttcttgactg tgccgtaggc tctgtgctcc
     1021 agttgctgtc gtttacgcag gaaaatgctc ccggctgata gactactccg gctattgagt
     1081 ccggaaaaga cgcgtgctga acgcgattca gaacaaccgc gcctactgca acctgaccta
     1141 tatacggttc gcctctcgct tcggctgcaa tcacctttgc aagcagatat atgtcgctcg
     1201 agctgtattg ccccgaggac gcagaaccga ttccgagtgc cgacaatgtt ttcggacccg
     1261 caatgccgtc ggcagtaaga cctgcggatt tttgaaactt ctttaccgcg tctttggtaa
     1321 gtgtgccgta aataccgtca acggatccgc tgtatattcc cttagatttc aacttggttt
     1381 gtatctgtct gacctcttcg ccgcgagagc ccgtttttga taacacttcg gtgctgtggc
     1441 ttgcactctg cttgtatacc gctatcgagc ctacggcaaa aagcagcacg gcggcaagac
     1501 acacggctaa catctttttg aattttgcac cgggagaatt catcttcata ataatttacc
     1561 gaccttttaa ctgtttaagt gctcgcttac ggaattattg ttttcatgtg tgacactttt
     1621 atacacataa attgtgtgtc acaaaaagac atatactaaa tattgacaga taaaatccgt
     1681 tttgaaaaaa atttgatttt tttaaaaata aatgttgact tttgggttgc gctgtgatat
     1741 tatatcaaag ctgtcgcgag agacacggaa acaaaattga acagaacgct tgataattca
     1801 acgtttttga ttaaacagtt tgaaaaaaag cacttgacaa tt
//
LOCUS       DBMDVO010000251         1842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30378_length_1842_cov_1.214819, whole genome shotgun sequence.
ACCESSION   DBMDVO010000251 DBMDVO010000000
VERSION     DBMDVO010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30378_length_1842_cov_1.214819"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="ACZPE2_04310"
     CDS             complement(<1..846)
                     /locus_tag="ACZPE2_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319107.1"
                     /translation="MKKRTLTVMLAATMLLAGTAFADEIKTPDTVTQTDAEAESMTAV
                     ATAEEIGDNYITGKTEQGTLIQFNISDDTVLLDSETGNAIGVSDIKKGDKFMVDYSSV
                     MTKSIPGQTNAYLIATNVDKGGAVNLINVTDVKENEDGSLTVTDKPAGIILTITKDAT
                     VKPYKTKNIVKLSDITIGTKLAAWYDFVALSYPAQATTNKVVIVDNEPLDSEFGVDTE
                     TMAEDTGITREAFSVLTYDMLDGAGKATLPRALSKGFSDVDNNKINALADMKIVSGRG
                     ENTFAP"
     gene            complement(936..1742)
                     /locus_tag="ACZPE2_04315"
     CDS             complement(936..1742)
                     /locus_tag="ACZPE2_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319108.1"
                     /translation="MQKIPFEKLYSADFEISDLFCMRQKWVEGVLFRMDHPRKSNGIL
                     YLKGCSGTYTFSGRSFEAPRKSIVYIPQGGIYTCLNRDCGHSVPDAYLVEFNLRLGGE
                     SAVLSDVPLLLDDFDPYIAELIKKAVSAYESPLRSPAAVRAAIYSLLSELGQDAKKDF
                     GKKYQSIAPGIRLLEENPFASYSVEELSAACGLSSGCFRRLFREYSGKSPVEYRNNLK
                     IEMAKSLLENSNATLEVVAESLGFESSAYFCRVFKKKTGMAPGEWRSVNK"
BASE COUNT          436 a          434 c          429 g          543 t
ORIGIN      
        1 cggtgcaaag gtgttttcgc ctctgccgga gactattttc atatcggcaa gcgcgtttat
       61 cttattgttg tcaacatcgg agaagccctt tgaaagcgct cttggcaagg ttgcttttcc
      121 ggcaccgtcc aacatatcat aggtgagaac ggagaatgct tctcttgtga tgcctgtgtc
      181 ctctgccatt gtctcggtgt caacaccgaa ttccgagtcg agaggctcat tgtcaacgat
      241 aacgaccttg tttgtggtgg cctgtgccgg ataacttaaa gccacaaagt cataccaggc
      301 agcaagcttt gtaccgatag taatgtctga aagcttaacg atgttctttg tcttataggg
      361 cttaacagtt gcgtcctttg tgatcgttaa gataattccg gcaggcttgt ctgtaactgt
      421 caggctaccg tcctcgtttt ccttaacatc cgtgacattg atgaggttga cagcccctcc
      481 tttatcaaca tttgttgcga taaggtatgc attagtctgt ccgggaatac tttttgtcat
      541 tacggatgag taatcaacca taaatttatc gcccttcttt atatcggaaa cgccgattgc
      601 gtttccggtt tcagagtcaa ggagcaccgt atcatccgag atgttgaact gaatgagcgt
      661 gccttgctct gtttttccgg ttatgtagtt atcaccgatc tcttctgcag ttgcgactgc
      721 cgtcatgctt tcggcctcgg catcggtctg cgtcacggtg tcgggcgtct tgatctcatc
      781 ggcaaacgcc gttcccgcta aaagcattgt agccgcgagc attactgtta atgttctttt
      841 tttcataaaa atcatccttt ctttttgtgg gctttgttac ccttttcacc tataatactt
      901 ttgaaagtac agaaaggttg caaaaaggtt gtgaactatt tgttaacact tctccattcg
      961 ccgggagcca taccggtctt ctttttaaat acgcggcaaa aataagccga gctttcgaag
     1021 ccgagcgatt cggcaaccac ttcaagcgtg gcgttactgt tttcaagaag gctttttgcc
     1081 atctcgattt taagattgtt tctgtattcg acgggactct tgccggagta ttccctgaaa
     1141 aggcggcgga agcacccgct gctcaggccg cacgcggctg agagctcctc cacagaatag
     1201 gaggcaaagg ggttttcctc caaaagtcgg atacccggtg caatcgactg atattttttg
     1261 ccgaagtcct ttttcgcgtc ctgtccgagt tcggacaaaa gagaatatat cgccgcgcgc
     1321 accgccgccg gcgaccttag aggcgactcg taggccgaca cggctttttt gatgagctcc
     1381 gcaatatacg ggtcaaaatc gtccaacaga agcggaacat ccgaaaggac ggcgctctcg
     1441 ccgcctaacc ttaaattgaa ctcaactaaa tatgcgtccg gcacggaatg gccgcagtcg
     1501 cggtttaagc aggtatatat cccgccctgg gggatatata cgatgctttt tctgggtgcc
     1561 tcgaacgacc ggccggaaaa ggtatatgtt ccggagcatc cttttaaata taaaatgccg
     1621 ttgctttttc gcgggtgatc cattctgaag agaacgccct cgacccattt ctgacgcata
     1681 cagaaaaggt cggatatttc aaagtctgca ctgtagagtt tttcgaaagg aattttttgc
     1741 atattatcac caccgcagtt cattttatca taactgttcg aaaagtgcaa taaaaatgtt
     1801 cggatagtgg attgatttat ttttggtaaa agctataatt at
//
LOCUS       DBMDVO010000252         1842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30380_length_1842_cov_1.037335, whole genome shotgun sequence.
ACCESSION   DBMDVO010000252 DBMDVO010000000
VERSION     DBMDVO010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30380_length_1842_cov_1.037335"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            116..1573
                     /locus_tag="ACZPE2_04320"
     CDS             116..1573
                     /locus_tag="ACZPE2_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319109.1"
                     /translation="MEKKESKAVETTAPHPNKIGIKEGIGYMLGDAGNLFVLTYVSSF
                     LKVFYTDVLKIATDKVATLFLVSRLWDAVNDPMWGTIVAKRRPNKDGKYRSYLKWLAI
                     PLALSQLVCFIDVRAITNKQWALLLFAYVTYIAFGMLYTGMNVPFGSLASVITDDPDG
                     RTLLSTFRSIGGGIGGAAPLLLAPFIIYTKVKNPDGSTYNVANPNGMFWFGAAMAACA
                     VIFYYACYANTKERVPSEAEPKVDMKKLYAGMFKSRPFIVLALTGILISGQLQFASLN
                     QYLYKNYFCNTSLSVIGTIAQYLPMAVMIPFVPKLVKKFGKKELSGFGAFFSALAAIA
                     CYIIKPRPDQAGIFIFLLFIIGFGYSFVSITNWAVVADVIDYQEYKTGIKNESAVYAV
                     YTFCRKLGQTAADYGGLMLLGKVGYDVQLMSNAGYVDGVSEGILKICTLIPAITYTLI
                     FLLYQFAYPLSKSKLEPVYDYVRNMNCAAQSRETY"
BASE COUNT          513 a          386 c          404 g          539 t
ORIGIN      
        1 acaaaaaatt gacaaatttt acactgaaat aaaacagatt tcacaaaaaa ttaattgctt
       61 tacattttat actatgttat actgtatttt acatcagtat aaacggggga gaataatgga
      121 gaaaaaagaa tccaaggcag ttgaaacgac tgctccgcat ccgaacaaaa taggcatcaa
      181 ggagggcata ggctatatgc tcggcgatgc cggaaacctt tttgttttaa cttatgtctc
      241 gtcattttta aaggtgtttt acaccgatgt acttaaaata gcgacagata aggtagccac
      301 attgtttttg gtttcgcgtc tctgggatgc ggtaaacgat cctatgtggg gaacgattgt
      361 tgcaaaaagg cgcccaaata aggacggtaa ataccgttcg tatctcaaat ggctcgcaat
      421 tccgcttgct ctctcgcagc ttgtgtgctt tattgatgtc agggcgataa caaataagca
      481 gtgggcactg cttttgttcg cgtatgttac atatatagcg ttcggtatgc tctataccgg
      541 aatgaatgtt ccgtttggct ctcttgcgtc tgttataacc gacgatccag acggcagaac
      601 ccttctttcg accttccgct caataggcgg cggaatcggc ggagcggctc cgcttctttt
      661 ggctccgttt attatttata ccaaggtcaa aaatcccgac ggatctacat ataacgttgc
      721 aaatcccaac ggtatgtttt ggtttggcgc ggctatggcg gcgtgtgcgg ttatattcta
      781 ttatgcctgc tatgctaaca ccaaagagcg tgttccctcc gaggcagagc ctaaggtgga
      841 tatgaaaaag ctgtatgccg gaatgtttaa gagccgaccg tttatagttt tagctcttac
      901 gggaatactg ataagcggtc agcttcaatt tgcttcgctc aatcagtatc tctataagaa
      961 ctatttctgt aatacaagcc tttccgttat aggtacaata gcacagtatt tacctatggc
     1021 tgttatgata ccgtttgtgc cgaagctcgt taaaaagttc ggcaaaaagg agctcagcgg
     1081 attcggagcg tttttctcgg ctcttgccgc aatagcgtgc tatattataa agccgagacc
     1141 cgatcaggca ggcattttta ttttcctgct gttcattata ggcttcggat attcattcgt
     1201 ttccataaca aactgggcgg ttgtcgccga cgttatcgac tatcaggaat ataagaccgg
     1261 aattaaaaac gagagtgcgg tatacgccgt ttataccttc tgccgtaaac tcggtcagac
     1321 agcggcggac tacggcggac ttatgcttct cggcaaggta ggctatgatg tgcagcttat
     1381 gtcaaacgca ggctatgtcg acggcgtaag cgagggaata ctcaaaatct gcacactgat
     1441 tcccgcaata acttatactc ttatattcct gctttatcag ttcgcatatc cgctttcgaa
     1501 gagcaagctc gaacctgttt acgattatgt tcgtaatatg aactgtgcgg cgcaaagcag
     1561 agaaacttat tgaataacaa taaaaaaata aaagcacaaa ccgaagcccc ataaaaggct
     1621 ccctttacaa tgaaacgcga cataagcttc aagctgtcag tcattctgct ctcgacccat
     1681 tccgaatggc agcatatggc gatagtggtg taaaccgttc tattggaaga caaaatggat
     1741 ataggcagga tactatttat gatgtcgtta gttctttgcc ttcagattca tcggttaatt
     1801 ttaatttgtt gtacaaccat aggcatcttt aaatatcatt tt
//
LOCUS       DBMDVO010000253         1832 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30629_length_1832_cov_1.284229, whole genome shotgun sequence.
ACCESSION   DBMDVO010000253 DBMDVO010000000
VERSION     DBMDVO010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30629_length_1832_cov_1.284229"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="ACZPE2_04325"
     CDS             <1..249
                     /locus_tag="ACZPE2_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319110.1"
                     /translation="DLKAVGNRGGSDALVVTFSNGGSLKSIQSYGGSRDDSFGGVCVL
                     SDGQIIACGSTYSDNGDLIGSKYLSSGSASMGMIARFK"
     gene            264..989
                     /locus_tag="ACZPE2_04330"
     CDS             264..989
                     /locus_tag="ACZPE2_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319111.1"
                     /translation="MKNYPGYLPQSHRILWLLIRVALMLWGIYGIFHGSVVEFLEAIF
                     AILFTHLWDYFQVFGGRSFIIRIGYENQTMLNVFMFVCVVIGSTLNNRTDFGSFDIVT
                     HTCSGFISAWFGYDFAVLMQEKNYSKLSPALAAMFGLCFSLGIDVGWEIYEFTMDRLY
                     GLSLQCSSPTTDYGLIDTMGDFICAASGALVGMFAVAFYRNGVIGKNKKEVRARLMRE
                     KEEEKLKRQLLDEYLKAHNDTNI"
     gene            1009..>1832
                     /locus_tag="ACZPE2_04335"
     CDS             1009..>1832
                     /locus_tag="ACZPE2_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319112.1"
                     /translation="MKKAVILLITVLTATLAFSSCNKKSSVSVNGVPVSEGVYNYYYD
                     IEKNSDEDKSQQEISDAALSDVAAYVAVNSEFKNRTLSLSSEDKNEISQNVNNYWHVF
                     SVYYNTIGVSKQDLQKIEESKKYKDAVMADYYSENGDEPVTDDELRSYFSENFIAFKA
                     VTGYLPSGSDTDELEKQALLQNFTQYAGAVNDGASIDEVGAALENVNTDSDTVVIGKN
                     DVSYPDGFFDSVSAIEPGKTGAFIAGDYVYIVSREDVSGNDNLFSTYRIKCLKEFCG"
BASE COUNT          476 a          399 c          461 g          496 t
ORIGIN      
        1 gacctcaagg cggtaggaaa ccgcggaggc tcggatgctc tcgttgtgac cttctctaac
       61 ggcggctcgc tcaagagcat acagagctac ggcggcagtc gtgacgactc gttcggcggc
      121 gtatgcgtgc tctccgacgg tcagataata gcctgcggct ccacatactc cgacaacggc
      181 gacctgatag gctcaaaata cctcagtagt ggctccgcaa gcatgggtat gatagcaaga
      241 tttaagtaaa ggacttttgg ttgatgaaaa attatcccgg ctatttgcct cagagccaca
      301 gaatactttg gcttttaatt cgcgtcgctt tgatgctttg gggaatttac ggtattttcc
      361 acggctccgt ggtggaattt ctcgaagcga tatttgcaat tctttttacg catctttggg
      421 actatttcca ggtattcggc ggcagatcgt ttattataag aatcggctac gaaaatcaga
      481 caatgctcaa cgtgttcatg tttgtttgcg ttgtaatagg ctctactctt aataacagaa
      541 ccgatttcgg cagcttcgac atagtaacgc acacctgctc cggctttatt tcggcatggt
      601 tcggctatga ttttgcggtg cttatgcagg agaaaaatta ttcaaagctt tctccggctc
      661 tcgcggcgat gttcggcctt tgcttctcac tcggcattga cgtcggctgg gagatatatg
      721 aatttaccat ggaccgcctc tacgggcttt cgcttcagtg ctcttcgccg acaacggatt
      781 acggccttat agacacaatg ggcgacttta tatgtgccgc aagcggagca cttgtgggaa
      841 tgttcgccgt ggcgttttac aggaacggcg ttatcggtaa aaataaaaaa gaggttcgcg
      901 caaggcttat gagagaaaaa gaagaggaaa agctcaaaag gcagcttctc gacgaatatc
      961 tcaaagcaca caatgatacc aatatctgat ttaacggagg ttcggcgatt gaaaaaagct
     1021 gtaattttgc ttataacagt tcttacggcg acattggcgt tttcgtcgtg caataaaaaa
     1081 agctccgttt cggtgaacgg cgtacccgtt tccgagggcg tttataatta ctattatgat
     1141 atagaaaaga attccgatga ggacaagtct cagcaggaga tttccgacgc cgcgctgtcc
     1201 gatgtcgcag cctatgttgc ggtaaattcg gaatttaaaa accgtactct ctcgctttcg
     1261 tccgaagaca aaaacgagat ttcgcaaaat gtcaataatt attggcacgt cttttccgta
     1321 tattacaata ccatcggcgt gtccaagcag gatctccaga aaattgaaga gagcaaaaaa
     1381 tacaaggatg ccgtaatggc ggattattat tcggaaaacg gcgacgagcc tgttaccgac
     1441 gatgaactgc gttcgtattt ttccgagaat tttattgcct ttaaggccgt aacgggctat
     1501 cttccgtcgg gaagcgatac cgatgagctt gaaaagcagg cgcttttgca gaattttacg
     1561 cagtatgcgg gtgccgttaa tgacggtgcg agcatagacg aggtcggagc ggctctcgaa
     1621 aacgttaaca ccgattccga taccgttgta atagggaaaa acgacgtttc gtatccggac
     1681 ggcttttttg acagcgtttc tgccatagaa cccggcaaga caggggcgtt tatcgcaggc
     1741 gactatgttt atattgtcag tcgtgaggat gtaagcggaa acgacaatct gttttcgact
     1801 tatcgtatta aatgccttaa ggaattttgc gg
//
LOCUS       DBMDVO010000254         1832 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30630_length_1832_cov_1.264587, whole genome shotgun sequence.
ACCESSION   DBMDVO010000254 DBMDVO010000000
VERSION     DBMDVO010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30630_length_1832_cov_1.264587"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            210..1523
                     /locus_tag="ACZPE2_04340"
     CDS             210..1523
                     /locus_tag="ACZPE2_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014680.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR1319113.1"
                     /translation="MQTFEIILSVISGLSLICALGALIFSKRAAKQSSSEDFAVLNSK
                     ISDLTKSTSDEFQRNRIETHQSLENMSAKLEAMTKLSYENREKMNETIAQSLNMIRDK
                     SIEQNERQSKIIETSIQKMQESNEKKLDQMRMTVDEKLTSTLTTRLDSSFKTVSEQLE
                     KLYKSLGEMKELSNGVTSNVTTLNRVLTNVKARGTWAEVQLKGILDQTIPTMYEENVA
                     TGEKATERVEFAVKIPSSEKGAEPTLLPIDSKFPMEDYIRLCDAADNADAAALAAARK
                     ALEDRILGEAKEIRKYINVPKTTPYAIMYLATEGLYAEIASSRSGLPEKLQNEYNIMI
                     AGPITITALLNSLSMGFKTVAINEKANEVRKLLAACKTQYDIFGTVLQKAKKKIDDAG
                     RSLDDAQHRNSIIQKKLRGVEDIERSEAENILSIDQGFADTEDDN"
     gene            complement(1620..>1832)
                     /locus_tag="ACZPE2_04345"
     CDS             complement(1620..>1832)
                     /locus_tag="ACZPE2_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003770872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR1319114.1"
                     /translation="AGAIFYILMMLYSLAVLLPSLSLMVRRLHDIGKSGTYILMGLIP
                     VVGYIFILVYMLTGSAPDNQYGPKKV"
BASE COUNT          607 a          413 c          401 g          411 t
ORIGIN      
        1 ggttgcaatt acacataaca ttatcaaaat tgaaattatt ttctttatca atttaaatta
       61 cccctataaa gtaatggtgt taatcaaatg tcgtttaaag cttatttcat tttaacacaa
      121 aaggctttta aaatccacac atttgttata ttgactcaag tctttatctc acctataata
      181 tattataaag aataaaggag aatgaattta tgcagacgtt tgaaataata ttgtccgtaa
      241 tttcaggcct gtcgcttata tgtgcgctcg gagccttgat tttttctaaa agggcggcaa
      301 agcaaagctc ctcggaggat tttgccgtat tgaattccaa aatatcagac cttacaaaga
      361 gtacatccga cgaatttcag cgcaaccgca tagaaacgca tcagagcctt gaaaatatgt
      421 cggcaaagct tgaggcgatg acaaagctca gctatgaaaa ccgcgaaaaa atgaacgaga
      481 ccatagcaca gtcgctcaat atgatacgcg acaagagcat agagcaaaac gagcgccaat
      541 cgaaaataat tgagacaagc attcagaaaa tgcaggagag caacgaaaag aagcttgacc
      601 aaatgcgtat gaccgttgac gaaaaactca cctcaactct tacaacacgt cttgacagct
      661 catttaagac cgtctccgaa cagcttgaaa agctgtataa atcgctcggc gagatgaaag
      721 agctttcaaa cggcgtcaca agcaatgtca ctactctcaa ccgtgttctt acaaatgtta
      781 aagctcgcgg cacatgggca gaggttcagc tcaagggaat acttgaccag acaataccca
      841 ccatgtacga agagaatgtt gcgacgggcg aaaaagcaac cgagcgcgtt gaattcgccg
      901 ttaaaatacc gtcgagcgaa aagggtgctg agccgacgct tctgccgata gattcaaagt
      961 tcccgatgga ggattatata cgcctttgcg acgcagccga caacgccgat gccgccgcat
     1021 tggccgccgc cagaaaggca ctcgaagaca ggatactcgg cgaagctaag gaaatacgca
     1081 aatacataaa cgtgccgaaa acaacgccgt atgcaattat gtatcttgcg accgagggac
     1141 tgtacgccga aattgcgtct tcgcgttcgg gactccccga aaagctgcaa aacgaataca
     1201 atattatgat agccggacca attaccataa cggcactttt aaactcgctg tcgatgggct
     1261 ttaagaccgt tgcgataaac gaaaaggcta acgaggtcag aaagcttctc gccgcgtgca
     1321 aaacacagta tgatatattc ggcacggtgc tgcaaaaagc aaagaaaaag atagacgacg
     1381 caggacgttc gcttgatgat gcacagcacc gcaattccat aattcagaaa aagctccgcg
     1441 gtgttgaaga tatagagcgc tccgaggcgg aaaatatatt gtcgatagac caaggcttcg
     1501 ccgataccga ggacgacaat taaaataatc ggcagaaaaa agccgggctg taaaggactt
     1561 ttgtccttta cagcccggtt ctttcattta acaatagata tacacgaccg aaatcacact
     1621 cagacttttt tagggccgta ctgattatcg ggggcgctgc cggtaagcat gtatacgaga
     1681 ataaatatgt agccgacaac cgggatgagc cccatcaata tatacgtgcc gctcttgcct
     1741 atgtcgtgaa gtcggcgcac cataagagac agcgacggca gtaacactgc aaggctatac
     1801 agcatcatca aaatataaaa tatcgcgccg gc
//
LOCUS       DBMDVO010000255         1831 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30674_length_1831_cov_0.858960, whole genome shotgun sequence.
ACCESSION   DBMDVO010000255 DBMDVO010000000
VERSION     DBMDVO010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30674_length_1831_cov_0.858960"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="ACZPE2_04350"
     CDS             <1..569
                     /locus_tag="ACZPE2_04350"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR1319115.1"
                     /translation="NLGGGFGIKYLESDTPKPFSEYMKRVSVVVKNEAEKKNVKLPFI
                     LIEPGRSVVGAEGVTVYTVGAVKHIPNIRTYISVDGGMTDNPRYILYQSDYDVMCANK
                     ANEPKTEKVTVAGKCCESGDLIQENAPIQKVEAGDLLAVLSTGAYNYSMASNYNRIPR
                     PIVIMAKDGESRIIIRRETYEDVTRNDV"
     gene            586..>1831
                     /gene="dnaE"
                     /locus_tag="ACZPE2_04355"
     CDS             586..>1831
                     /gene="dnaE"
                     /locus_tag="ACZPE2_04355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1319116.1"
                     /translation="MSFTHLHLHTEYSLLDGTCRIGQLVDAAKELNMDSLAITDHGNM
                     FGAVEFYKAAKENGIKPIIGCEVYVAPRSRLDKVHGIDNERFHLVLLCKNNTGYENLI
                     KIVSESWVTGFYTKPRIDKQLLEKYHEGLIALSACLAGEIPRKLLQDDYDGAKEAALW
                     YDKTFGRGNYYLEIQNHGIAEQLRVLPQIVKISRETGIPLVATNDVHYIKKENAKVQK
                     VLICIQTNHTIDEDTGLEFGTDDFYLKSEDEMRELFGDYSDAVDNTSVIADMCNVEFE
                     FHKTKLPHFDVPDNMDHFEYFRNECYKGLRRNYGIAPEQKYIDRLEYELSVINKMGYV
                     DYFLIVADFISYAKRHGIPVGPGRGSGAGSIAAYCMGITGIDPIKYNLLFERFLNPER
                     VSMPDIDVDFCYERREEVIDYVI"
BASE COUNT          562 a          326 c          413 g          530 t
ORIGIN      
        1 tcaacctcgg cggaggattc ggcattaagt atcttgaatc ggataccccg aagccgtttt
       61 cggaatatat gaagagagtt tctgttgttg tcaagaatga agcagagaag aaaaatgtta
      121 agcttccgtt tatacttatt gagcccggtc gttctgtggt cggagcagaa ggcgttaccg
      181 tttatacagt cggagccgtt aagcatattc cgaatatcag aacctatatt tcggtagacg
      241 gaggaatgac agacaatccg cgctatattc tctatcaatc ggattatgat gtaatgtgcg
      301 ccaataaagc aaacgagcct aagactgaaa aagtaaccgt tgccggtaaa tgctgcgaaa
      361 gcggtgacct tatacaggaa aatgctccaa tacaaaaagt tgaggcagga gaccttttag
      421 ctgtgctttc aacaggtgct tacaactact caatggcttc aaattataac agaattccgc
      481 gtcctatagt aataatggca aaggatggag aatcgcgtat aattataaga cgtgaaacat
      541 acgaagatgt tacacgtaat gatgtttaaa atgaaaggaa tttaaatgtc ttttactcat
      601 ttgcatcttc atacagaata cagtctgctc gacggcacct gccgtatagg acagcttgtt
      661 gatgctgcaa aagagctcaa catggattct ttagccataa cagaccacgg aaatatgttc
      721 ggtgccgttg agttttataa agcagctaaa gagaatggga taaagcccat tatcggttgt
      781 gaagtttatg ttgctccacg ttcgcgcctt gataaagttc acggtattga taacgagcgt
      841 tttcatcttg ttttgctctg taaaaataat acgggatatg aaaatcttat caaaattgtg
      901 tctgaatcgt gggtgacagg attttataca aagccgcgta ttgataaaca gctcctcgag
      961 aaatatcacg aggggcttat tgctctgtct gcctgtcttg cgggagaaat tccgagaaaa
     1021 ctgttgcaag acgattatga cggagctaag gaagctgcac tttggtatga caagactttt
     1081 ggtcgcggca attattatct cgaaatacag aatcacggaa ttgccgagca actcagggtt
     1141 ttgcctcaga tagttaaaat ttcgcgtgaa accggtatac cgcttgtcgc tacaaacgat
     1201 gtccattata taaaaaaaga aaacgcaaaa gtacaaaaag ttcttatctg tattcaaact
     1261 aaccatacaa tagatgaaga tactggtttg gaatttggaa ccgatgattt ttatctcaaa
     1321 tccgaagatg aaatgcgtga attattcgga gattattcgg atgcggttga taatacttct
     1381 gttatagctg atatgtgcaa cgttgagttt gagtttcaca aaacaaaact accgcatttt
     1441 gatgttcccg ataatatgga ccattttgaa tattttcgta atgagtgcta taagggcttg
     1501 cgcagaaatt acggaattgc tccggagcag aaatatatcg acagactcga atacgaactt
     1561 tcggttatca ataaaatggg ctatgttgac tattttctta ttgttgccga ttttatcagc
     1621 tatgcaaaac gccatggtat tcccgtaggt cccggcaggg ggtcgggagc cggcagtata
     1681 gccgcttatt gtatgggaat tacaggtata gacccaatta aatacaatct gctgtttgag
     1741 cgttttttaa atccggagcg tgtaagtatg ccggatattg atgttgattt ttgttatgaa
     1801 cgccgcgaag aagtaattga ttacgttata a
//
LOCUS       DBMDVO010000256         1828 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30734_length_1828_cov_1.182976, whole genome shotgun sequence.
ACCESSION   DBMDVO010000256 DBMDVO010000000
VERSION     DBMDVO010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30734_length_1828_cov_1.182976"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="ACZPE2_04360"
     CDS             complement(<1..792)
                     /locus_tag="ACZPE2_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR1319117.1"
                     /translation="MADVKKVNNANDENLDELHMIRVQKLRDLQAEGNDPFVITKCDV
                     THHSADIKADFDDLDGKEVSVAGRLMSKRIMGKASFCNVQDLKGSIQSYVARDCIGED
                     EYKAFKKMDIGDIVNIKGTVFKTKTGEISIHATQVVLLSKSLQVLPEKFHGLTNTDTR
                     YRQRYIDLIMNQDVKDTFVKRSRIISSIRNYLDSEGFMEVETPMLVANAGGAAARPFV
                     THYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGLDTR"
     gene            complement(865..>971)
                     /locus_tag="ACZPE2_04365"
     CDS             complement(865..>971)
                     /locus_tag="ACZPE2_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR1319118.1"
                     /translation="SPVGKALIGHKVGETVEVEAPIGLLKFVIKEISK"
     assembly_gap    972..981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<982..1351)
                     /gene="greA"
                     /locus_tag="ACZPE2_04370"
     CDS             complement(<982..1351)
                     /gene="greA"
                     /locus_tag="ACZPE2_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1319119.1"
                     /translation="MAQQETLLTAEGLRDLEKELEELKTVGRKEAAEKIKVARSFGDL
                     SENSEYDEAMSDQAKLEARINEIEHMIKNAKVFDEEALGTEVVHIGSKVTITNDSGAE
                     LQYKIVGFAQADPDEGKISDE"
     gene            complement(1472..>1828)
                     /locus_tag="ACZPE2_04375"
     CDS             complement(1472..>1828)
                     /locus_tag="ACZPE2_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR1319120.1"
                     /translation="LDGGHNPECAAALENVLVRFAGDKSITVLIGMMADKDTKDYLAA
                     ILPHCKTAVFTKPSNPRAEDENVLLREGKCFCRDVVSVKDPKDAYKKARSLVKNGDML
                     LVCGSFYLLSDIFGEP"
BASE COUNT          449 a          457 c          373 g          539 t
ORIGIN      
        1 gcgtgtgtca agaccctcat tacggaaaac tctgcctatc tcatacactc tctcgagtcc
       61 gccgactata aggcgtttca gatacagctc aagagaaatt ctgagcttaa cgtcctcgtc
      121 gagagcattg tagtgagtga caaacggtct tgccgccgcg ccgcctgcgt ttgcaacgag
      181 catgggggtt tcaacctcca taaagccctc tgaatcaaga taatttctga tactgctgat
      241 tattcttgaa cgctttacaa acgtatcctt tacgtcctga ttcataataa ggtcgatata
      301 tctctggcgg taacgggtat cggtattggt aagtccgtgg aacttttcgg gaagaacctg
      361 aaggctcttc gaaagcagta ccacctgcgt tgcgtgaatc gaaatctcgc ctgttttggt
      421 tttgaaaacg gtacccttga tatttactat atcgccgatg tccattttct taaaggcttt
      481 atattcatcc tcgcctatgc agtcgcgggc aacatacgac tgtatactgc cctttagatc
      541 ctgtacattg caaaatgacg ccttacccat aatacgcttt gacataagtc tgccggcaac
      601 ggaaacctcc ttgccgtcaa gatcatcaaa atcggcttta atatccgcgc tgtggtgagt
      661 aacatcgcac tttgtgataa caaacgggtc attgccctcg gcttgtaaat cacgaagctt
      721 ctgaactctt atcatatgca gctcgtcgag gttttcatcg ttagcattgt ttactttctt
      781 tacgtctgcc atatcttcac ttcctgtcaa aaaaggagcg caagggaaca aagagagtcc
      841 cttttcgccc cgttaaattt ttaattattt tgaaatttcc ttaatgacga atttaagaag
      901 accgataggt gcttcaacct caacggtctc gccgaccttg tggccgataa gggctttgcc
      961 tacgggcgat tnnnnnnnnn nattcgtccg atattttacc ctcgtcggga tctgcctgtg
     1021 caaagccgac tattttatat tgaagttctg cgccggaatc gtttgtgata gtgacctttg
     1081 agcctatatg aacaacctct gttccgagag cctcctcgtc aaaaaccttt gcgtttttga
     1141 tcatatgctc aatttcgttt atgcgagcct cgagctttgc ctgatcgctc attgcctcat
     1201 cgtactcgct gttctccgac aagtcgccga agcttctcgc aactttaatt ttttccgcag
     1261 cttcttttct gccgacggtt tttaattcct cgagttcttt ttcaagatcg cgaagaccct
     1321 ctgcggtcaa taaggtttcc tgctgagcca tactaattca ccatagcctt tcaagtttat
     1381 aaatcgtacc gcaaataaaa tacgcggccg ttaaatttca gtattacacg aataaatatt
     1441 gtatcaaaag agcaaagata tgtcaagttt tttagggctc tccgaaaatg tcggaaagca
     1501 ggtaaaacga accgcacaca agcagcatat cgccgttttt cacaaggctc cttgcctttt
     1561 tgtaagcgtc tttcgggtct tttacggaaa caacgtccct acaaaagcac ttaccctcac
     1621 gaaggagcac attctcatcc tcggcgcgcg gatttgacgg ctttgtaaac accgcggttt
     1681 tgcaatgcgg caaaattgct gcaaggtaat cctttgtgtc cttatcagcc atcataccta
     1741 taagaacggt tatgctttta tcgcccgcaa accgcacaag gacgttttca agtgccgcgg
     1801 cgcactccgg attgtgtccg ccgtcgag
//
LOCUS       DBMDVO010000257         1819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_30935_length_1819_cov_1.432480, whole genome shotgun sequence.
ACCESSION   DBMDVO010000257 DBMDVO010000000
VERSION     DBMDVO010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30935_length_1819_cov_1.432480"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            705..884
                     /locus_tag="ACZPE2_04380"
     CDS             705..884
                     /locus_tag="ACZPE2_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319121.1"
                     /translation="MFNKNFWLITLCALLIISIYTGLNIFTRIALGANGLIILADVII
                     SVRRLAKEKKCKESV"
     gene            complement(1064..1138)
                     /locus_tag="ACZPE2_04385"
     tRNA            complement(1064..1138)
                     /locus_tag="ACZPE2_04385"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1104..1106),aa:Gln,seq:ttg)
     gene            complement(1211..1287)
                     /locus_tag="ACZPE2_04390"
     tRNA            complement(1211..1287)
                     /locus_tag="ACZPE2_04390"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1251..1253),aa:His,seq:gtg)
     gene            complement(1297..1373)
                     /locus_tag="ACZPE2_04395"
     tRNA            complement(1297..1373)
                     /locus_tag="ACZPE2_04395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1337..1339),aa:Arg,seq:tct)
     gene            complement(1522..1734)
                     /locus_tag="ACZPE2_04400"
     CDS             complement(1522..1734)
                     /locus_tag="ACZPE2_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319122.1"
                     /translation="MFDPLNQNGDDEIDFSEKNLFEGLPVDEICKNTVDFPEEEFKKP
                     EKGEEEGKKIPDNLTVSEEQKPHLVI"
BASE COUNT          536 a          385 c          381 g          517 t
ORIGIN      
        1 ctctgaaaga accccaatcg ccgtatcttg cgatatgacg gttggggtat actggataag
       61 aataaatctc caaaggggat gaatggtatg aatatgtact tttctgcaag aacgcagacg
      121 ctggcacagt tcaaggaagc ctatgacaac ggcaaaaatc cggaacttgt cttatataag
      181 aaaagatgac aaataggact ctcccgtcag gagctgtcac cacaggtgac tgagggatta
      241 aattaggctc ccctaaaaag ggtgattccc cccacagtgt gggaaacgtc gcgaagcgac
      301 aaaaaaggct cccttgcaaa gggagctggc tgtgagcaat gcgaacagag tgaggtatta
      361 aaaaaccaaa gtatttctta tttaaaatct attttttaag gaaatccctc cgagttgcct
      421 tacggcaacc cacctccctt tacaagggcg gcaaatccct ccggcttgcc ttaacgaggc
      481 ccacctccat tttaagggag gcacgtcggc gagaaaagtg aacggtaaaa aagcaaaatc
      541 aaaagcctac cgcctttgaa attgcatatc ttgttatcga tgcaatcgtt gcacttgcga
      601 ctttgataac ggcaatcaaa tggtggtagg ctacatagag gggcttttgc ccctccccct
      661 tgcgggagta tatataatat aacacataga aaggagtttt acaagtgttc aataaaaact
      721 tttggttgat tactctttgc gcattgctta taatttcgat atataccggg cttaatatat
      781 ttacgagaat tgcactcggt gcaaacggct tgattatatt agccgatgta attataagtg
      841 ttcgcagatt ggcaaaagag aaaaagtgca aagagagcgt ttaaaactct tagcacatcg
      901 caagttttga cttgcatttg acttgcattt tttcttttaa atagccgcaa atggcacaaa
      961 atggcgcaaa aacgggtgac attttgatga ttaaaaaacg ccgaaagccc ttgatacaca
     1021 aggcttttca gcgtttttgt tgccgctttc aaaaacggcg tcgtggctgg ggtggcagga
     1081 tttgaaccta cgaatgcagc agtcaaagtg ctgtgtctta ccgcttgacg acaccccaat
     1141 atttactttt ttaaaattat accttaataa acgtaaatct gcaagagaaa tatcttgcag
     1201 atttagaaag tggggtggat agtcggattc gaaccgacgg tctccagtgc cacaaactgg
     1261 cgctttaacc aactaagcta tacccaccat attaactggc gcgcttggag ggactcgaac
     1321 ccccgaccct ctgcttagaa ggcagatgct ctatccagct gagctacaag cgcataacag
     1381 gtacactttt aagtgcttga atattatata tgcaacaggc gtttttgtca agtacattct
     1441 cgtgatttgt cgtcgaaaat atagcataat ggcggcgaag ccgaaacgac ggtttatcgc
     1501 cctgtttata aaaatattat gttaaatcac caaatgaggt ttttgctctt cagataccgt
     1561 taaattatcg ggtatctttt ttccttcttc ttctcctttt tcgggctttt tgaattcctc
     1621 ctccgggaaa tcgactgtat ttttgcaaat ctcatcaacc gggagtcctt caaacaagtt
     1681 cttttcggaa aaatctattt catcgtcgcc gttttggtta agcggatcaa acatttttta
     1741 caccctctta cttatcaatc aaatgtacat tgagatgatt atacgtcatt tcaatgcctg
     1801 cgttatcaaa ttcggtctt
//
LOCUS       DBMDVO010000258         1813 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31092_length_1813_cov_1.069509, whole genome shotgun sequence.
ACCESSION   DBMDVO010000258 DBMDVO010000000
VERSION     DBMDVO010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31092_length_1813_cov_1.069509"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="ACZPE2_04405"
     CDS             complement(<1..627)
                     /locus_tag="ACZPE2_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1319123.1"
                     /translation="MQYSKVEICGVDTSKLRVLKEKEKLELLKKIREGDKSAREKLIN
                     GNLRLVLSVIQRFTNRGENLDDLFQVGVIGLIKAIDNFDVSLKVRFSTYAVPMIIGEI
                     RRYLRDNNTVRVSRSVRDTAYHALGAKEKLQAKLGRDPTVDEIAKELGTSRSDVVLAL
                     EAIVEPVSLFEPVYSDGGDTIYVMDQVGGSETDTDWLDEISIKESIKEL"
     gene            1018..1359
                     /locus_tag="ACZPE2_04410"
     CDS             1018..1359
                     /locus_tag="ACZPE2_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319124.1"
                     /translation="MIATKQFGHRKEDYILTIISSMDMPTYVGILLIMFVLSIYSIVK
                     YRKIKEPWKVLIYLAPPEFIVFIANRIIKEYWSNTHLYNISSTVALITGGIVMTSIFV
                     VGIITKVKKKR"
     gene            1409..1618
                     /locus_tag="ACZPE2_04415"
     CDS             1409..1618
                     /locus_tag="ACZPE2_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319125.1"
                     /translation="MLAIFVLICNTYLNGQAVTYDEIGNPLTFGSKSFEWCGRQLERI
                     TDGDNTYVYSYNADGDRVSKTVNGV"
BASE COUNT          521 a          365 c          364 g          563 t
ORIGIN      
        1 caattccttt atgctctcct taatggatat ttcgtcaagc caatcggtat ccgtctcact
       61 tcctccgacc tgatccataa catatatggt gtctccgccg tcagagtata caggctcaaa
      121 gagcgaaacc ggctccacta tcgcctcaag tgctaatact acgtcagaac ggctcgtgcc
      181 cagctctttt gctatttcgt caaccgtcgg gtctctgccg agctttgcct gtaatttttc
      241 ttttgccccg agggcgtggt atgccgtatc tcttacagag cggctgacgc gcaccgtgtt
      301 gttgtccctg aggtatcgcc gtatttcacc gatgatcatg ggaaccgcat aagtggaaaa
      361 gcgcactttt aacgacacgt cgaagttatc gatcgccttt ataagcccta ttacgccgac
      421 ctgaaaaagg tcgtcaaggt tttctccgcg gtttgtaaaa cgctgtatca cacttaacac
      481 aaggcgcaaa ttgccgttta tcagtttttc gcgcgcagac ttatcacctt ccctgatttt
      541 cttgagaagc tcaagctttt ccttttcctt aagaaccctg agcttagagg tatcgacacc
      601 gcaaatctca accttgctgt actgcatggc gctctctcct ataactattg tcattaggat
      661 tattgccgtg cagctcactc ttaatacaaa acgcaccgtg aaaaatcgcg gccgattttt
      721 tggtgatatt tatttaaggt taaatatcgt ctccggcagc gaataaaagt actgtaacca
      781 aaaatcgaca catatcgtct gcttttttgt tgactttttt tatacattct gttaatgtta
      841 atatgaaaac ataccgttac ggtaaacaac gtttaaaata cagctgaacg ggagtgcaag
      901 ccccatgcgg taaaatttca cggcttgata aaaacgtgcg gcaaattaaa aactttaaat
      961 ttacgcacgg ataatatcta tgatattgtc cgttttttat ttttcggcga attgtttttg
     1021 attgcaacaa aacaattcgg gcaccgaaag gaggattata tattgaccat aataagttct
     1081 atggatatgc cgacatacgt aggcatattg ctaataatgt ttgttttatc tatttattct
     1141 atagtaaaat atagaaagat aaaagagcct tggaaagttt taatttattt agctccgcca
     1201 gaatttattg tctttatcgc aaacagaatt ataaaagaat attggtcgaa tactcattta
     1261 tataatatca gttctacagt tgctttaata actggtggta tagtaatgac ttctatattt
     1321 gttgtaggaa ttattacaaa ggtgaagaaa aagcgttaaa acgatattat tcgcctacac
     1381 ttattacttg tcggcgcatt ttaggcgttt gttagctata tttgtactga tatgtaacac
     1441 ataccttaac ggtcaggctg taacctatga tgaaatcggc aatccgctca ctttcggcag
     1501 caagtcgttt gagtggtgcg gcaggcagct tgagcgtata accgacggtg acaacacata
     1561 tgtttacagc tacaatgccg acggcgacag agtaagtaaa accgtaaacg gcgtatagga
     1621 agctggctca ataatattgg ttattgggcg caagagaaaa acttgggaaa ggagtatgaa
     1681 tgggaggcta attatgtata taaaacgaaa tgggagtaga attatacgtt taatcgtgct
     1741 tataatagca attttaatcg cgcttctaat agcaatactt atgttagcat ttagtcaagc
     1801 ttttcaggat tgg
//
LOCUS       DBMDVO010000259         1812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31116_length_1812_cov_0.978375, whole genome shotgun sequence.
ACCESSION   DBMDVO010000259 DBMDVO010000000
VERSION     DBMDVO010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31116_length_1812_cov_0.978375"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="ACZPE2_04420"
     CDS             complement(<1..663)
                     /locus_tag="ACZPE2_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014420.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1319126.1"
                     /translation="MIKDITIGQFFPGNSVIHKLDPRIKLVLTFVYIVIVFLCKNFVS
                     LASIVVALIVVILLSKISPKLIWKSVKPIFIIIAITSLLQIFYNKDGTVLFTIGKFSL
                     YTGGLYMAIFTTVRIICLVIASSMLTYTTSPTMLTDAMERLFSPLKVIKINVHSIAMM
                     MTIALRFIPTLIDEVDKIMSAQKARGADIESGGIIKRGKALVPIFIPLFINSFRRAYE
                     LAF"
     gene            complement(660..>847)
                     /locus_tag="ACZPE2_04425"
     CDS             complement(660..>847)
                     /locus_tag="ACZPE2_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1319127.1"
                     /translation="TVNEVFSKVERLADIGLNVPQLTQIFLNLKHNGMAVRTDIYTIK
                     NAEAELLRLLREEGVLS"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<858..1451)
                     /locus_tag="ACZPE2_04430"
     CDS             complement(<858..1451)
                     /locus_tag="ACZPE2_04430"
                     /inference="COORDINATES: protein motif:HMM:NF010158.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1319128.1"
                     /translation="MNNVNISIEKGEFVGIIGHTGSGKSTLVQHLNGLLTPSSGEVLL
                     DGKNINESKITRRQARFKVGLCFQYPEYQLFESTVYKDISFGPKNMGLSEDEIDNRVK
                     KAADFVGLKPDMLNKSPFDLSGGEKRRVAIAGVMAMEPEILILDEPSAGLDPRGRDMI
                     AEMISSYRKTTGSTVIIVSHSMEDVAKSADKVLVMNKS"
     assembly_gap    1488..1497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1522..>1812)
                     /locus_tag="ACZPE2_04435"
     CDS             complement(1522..>1812)
                     /locus_tag="ACZPE2_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with CbiNQ forms the ABC transporter for cobalt
                     import; Mycoplasmas have two adjacent copies of this gene;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1319129.1"
                     /translation="IKLNREMGMTVVFITHFMEEAVLANRVIVMDDAKIILDGTPKQV
                     FHNSELLKKVGLDVPETAKLANDLRESGAPLPEDILSADEFSDEILKLLRRK"
BASE COUNT          553 a          386 c          308 g          545 t
ORIGIN      
        1 aaacgcaagt tcatatgccc ttctgaacga atttataaat aagggtatga atatgggaac
       61 aagtgctttt ccgcgcttga taattccgcc gctctctata tcagcacctc tcgccttttg
      121 agctgacatt atcttatcaa cttcgtcaat aagagttggt ataaatctta aggctatcgt
      181 catcatcata gctattgagt gaacgtttat tttaataacc tttagcggcg aaaacagacg
      241 ttccatagcg tcggtaagca tagtgggaga cgtagtgtaa gtaagcatag agcttgctat
      301 aacaagacat ataattcgta ccgtagtaaa aatagccata taaagcccgc cggtatataa
      361 cgaaaattta ccgattgtaa aaagcacagt tccgtcttta ttgtagaaaa tctgtaaaag
      421 cgacgtaata gcaataataa taaatatcgg ttttacactt ttccaaatca gcttcggtga
      481 aatcttagat aaaagtatta ccactattaa cgctactaca atggaagcaa gagaaacaaa
      541 gtttttgcac agaaaaacaa taacaatata tacgaaagtt aaaacaagtt ttattctcgg
      601 atcaagcttg tggataactg aattacccgg aaaaaactgt cctattgtta tatccttaat
      661 cacgataaaa cgccctcctc tcgcaaaagg cgcaatagct ctgcctcggc gtttttaata
      721 gtatatatat ctgtacggac agccataccg ttatgcttta agtttaaaaa tatttgtgtt
      781 aactgcggaa cgttaagtcc tatatccgca aggcgctcaa ccttggagaa tacctcgttt
      841 acagtgcnnn nnnnnnngga tttattcata acgagcacct tatcagcact tttcgcaaca
      901 tcttccatac tgtgagaaac aataataacc gtgcttccgg tagtcttacg atagcttgaa
      961 atcatttcag ctatcatatc gcggccgcgc ggatcgagtc ccgccgacgg ctcatcaaga
     1021 atcaaaattt cgggttccat agccatgaca ccggctatgg ctacacgtct tttttctccg
     1081 ccggacaaat caaaaggaga cttatttaac atatccggct ttaatccgac aaaatcagcg
     1141 gcttttttca cacgattatc aatttcatct tcagacaatc ccatattttt ggggccaaaa
     1201 gagatatctt tatatacggt actctcgaat aattgatatt caggatattg gaaacacaga
     1261 cctactttaa atcttgcctg tctacgtgtt atttttgatt cgtttatatt tttcccgtca
     1321 agcaaaacct cacccgaaga tggcgttaaa agcccgttca aatgctgaac caatgtactt
     1381 tttcccgaac ctgtgtgccc tattatgccg acaaattcgc ctttttcaat agatatattt
     1441 acattattta ttgcagttac gcgaaaaggc gttccgtccg aataaacnnn nnnnnnnggt
     1501 tttttgtttc taaaatgggc attattttct cctaagtagt tttaatattt catcagaaaa
     1561 ttcatcagcc gaaagtatgt cttcaggaag aggagctccg ctctcacgca agtcattcgc
     1621 aagcttcgcc gtctccggaa cgtcaagacc gacttttttt agaagctccg aattatgaaa
     1681 aacctgtttc ggtgttccgt ccaaaattat ttttgcatca tccattacaa taacacggtt
     1741 ggctaaaact gcttcttcca taaaatgagt tataaacaca acggtcatac ccatctctct
     1801 gttaagcttt at
//
LOCUS       DBMDVO010000260         1810 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31159_length_1810_cov_1.613224, whole genome shotgun sequence.
ACCESSION   DBMDVO010000260 DBMDVO010000000
VERSION     DBMDVO010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31159_length_1810_cov_1.613224"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="glf"
                     /locus_tag="ACZPE2_04440"
     CDS             complement(<1..802)
                     /gene="glf"
                     /locus_tag="ACZPE2_04440"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1319130.1"
                     /translation="MKQYDYLIIGSGLFGSVFAHEATKHGKKCLVIDKRSHLGGNIYC
                     EDIEGITVHKYGAHIFHTNDKRVWTYVNDLVEFNRYTNSPVANYYGELYNLPFNMNTF
                     NKMWGVVTPEEAKAKIEEQKKQVTGEPKNLEEQAISLIGYDIYKKLIKGYTEKQWGRE
                     CKDLPAFIIKRLPVRFTYDNNYFNDRYQGIPIGGYNKLIEKLLEGIDTRLDTDFLKDR
                     EALSALADTVVYTGPIDQYYDYRFGKLEYRSLRFENELLDCENYQGVAV"
     gene            complement(855..>1810)
                     /locus_tag="ACZPE2_04445"
     CDS             complement(855..>1810)
                     /locus_tag="ACZPE2_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1319131.1"
                     /translation="NDFKLISVLRDSCVAIDGHGNQKITHAYAYGGAELAIKTLNQNF
                     NMNITDYATINFYKLAEAIDILGGVDIDITEDERLELNNIGDDDNPNFQYVEKSGMVH
                     LTGEQASVYSRIRKRDSDNARVDRQKKVIECLIDKARNISPTKYADLVKAGMALCETS
                     MSVPEVLSFAPMLSNDITIETMVVPGDEDNAVGGIYDGAWVWRYDLAAAADRMHLFIY
                     GEAPETTASSKKKQESQTTTKSSKSSSSATKSSGSSNGGSKSKSSATTSAKHSGGNNK
                     SETTTKPQTTAEATKNSETTKEHTTAETKTTASSDETKSAA"
BASE COUNT          393 a          435 c          417 g          565 t
ORIGIN      
        1 taacggctac accctgatag ttttcacagt caagcagttc gttttcaaaa cggaggctgc
       61 ggtattcgag cttgccgaag cgatagtcgt aatactggtc tatggggccc gtgtagacaa
      121 cggtgtccgc aagggcggaa agagcttccc tgtcctttaa gaaatcggta tcaagacggg
      181 tgtcgattcc ttcaagcagc ttttcgataa gcttattgta gccgcctatc ggaataccct
      241 ggtatctgtc gttaaaatag ttgttatcat aggtgaatct gaccggcaga cgctttatta
      301 taaatgcagg caaatccttg cattctctgc cccactgctt ttcggtatag ccctttatga
      361 gctttttgta aatgtcatat ccgataaggc ttattgcctg ctcctcgaga tttttgggct
      421 cacccgtaac ctgcttcttc tgctcctcga ttttggcttt tgcctcctcg ggggtcacaa
      481 caccccacat cttgttaaag gtattcatat taaacggcag gttataaagc tctccgtaat
      541 aatttgcaac cggggaattt gtgtagcggt tgaattcaac aaggtcgttt acataagtcc
      601 aaactctctt gtcgtttgtg tggaatatat gcgcgccgta tttgtgcacg gttatgccct
      661 caatatcctc gcaataaata tttccgccga gatggcttct tttgtcgata acaaggcact
      721 ttttaccgtg ttttgtcgct tcgtgggcaa aaacgcttcc gaaaagtccg ctgcctatga
      781 taagatagtc atactgtttc ataatttttc ctcctgcgcc gaagcgcaat taaaaaatgt
      841 attgtttgaa tttatcacgc cgccgactta gtttcgtcgg acgatgccgt tgtcttggtt
      901 tcagccgtag tgtgctcttt ggtagtttcg ctgtttttgg ttgcctcggc ggttgtctgc
      961 ggttttgtcg tggtttcgga tttattattg ccgccgctgt gcttagccga tgtcgtggcg
     1021 gaacttttgc tttttgaacc gccgtttgaa gagccgctgc tctttgttgc ggacgaagag
     1081 gattttgagc tcttggttgt tgtctgcgac tcttgttttt tctttgacga cgcagttgtt
     1141 tccggggctt cgccgtaaat gaacaggtgc attctgtcgg ctgccgccgc aagatcgtat
     1201 ctccataccc atgcgccgtc gtaaataccg cctaccgcgt tatcctcgtc gccgggaaca
     1261 accatagtct caatggttat atcatttgaa agcatcggag cgaaagagag aacctccgga
     1321 accgacatcg atgtttcaca aagagccata cccgccttta ccaaatcggc atactttgtg
     1381 gggctgatgt ttctcgcctt gtcgataagg cactcaataa cctttttctg cctatccaca
     1441 cgggcgttat ccgaatcgcg ctttctgata cgcgaataca cggacgcctg ctcgcccgtc
     1501 aggtgcacca tacccgattt ttcgacatat tggaaattcg gattatcgtc gtcaccgata
     1561 ttgttaagct caagacgctc gtcctcggtt atatcaatat ctactccgcc gaggatatct
     1621 attgcctcgg caagcttata aaagtttatc gtggcataat cggtaatatt catattgaaa
     1681 ttctggttta gcgttttaat ggcaagctcc gctccgccgt aagcataggc gtgagttatt
     1741 ttctgattgc cgtgcccgtc tatcgcaaca cagctgtcac ggaggacgga aatcagctta
     1801 aagtcgtttt
//
LOCUS       DBMDVO010000261         1805 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31269_length_1805_cov_1.058099, whole genome shotgun sequence.
ACCESSION   DBMDVO010000261 DBMDVO010000000
VERSION     DBMDVO010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31269_length_1805_cov_1.058099"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="ACZPE2_04450"
     CDS             complement(<1..369)
                     /locus_tag="ACZPE2_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HIR1319132.1"
                     /translation="MAVDFEFKSKYNVDDLLSIMRILREPGGCPWDMEQTHESIKKNF
                     IEETYEVVEAIDKKDKELLCEELGDVLMQVVFHAEMERENGSFDFSDVADGVCKKLIE
                     RHPHVFGDVTVKDSGEVLQNW"
     gene            complement(394..>1805)
                     /locus_tag="ACZPE2_04455"
     CDS             complement(394..>1805)
                     /locus_tag="ACZPE2_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015013.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR1319133.1"
                     /translation="PAIIAITPSIFFCCMMSIYRGYYNGLRNMNPTAISQVVEAIGKM
                     IFGIAAAKLTITYGYSQFESGAKVFGKVCATESEAMSAIYPYAAAAAAVGVTMGTVIG
                     MIYMIIMHKAKGDGITRTEIVNSPRPVNSGAIAKTLVAIAIPVVTSSIIFSLTNLIDA
                     ITIQNRLDGVISNNLDLIKSIYATQIAEAHVLDADLKDFLYGAYTLSLDFKNLIPSIT
                     TTLGVSAIPALSAAYAVKDKRALKSSVESVLRVGMIISLASGIGMGVLAEPILRMFYE
                     NGKSAPAISIAAPIMAAYGYTIFLMAISQPMTNMLQAVGKANVPVKSLTVGAVVKVVA
                     NYIFIGIPSININGAVIGTVLCYLIVVIYNYIALIKATKVKVNLMSVVVKPLISSVLC
                     GVAAYCAYGISYKILPQRLLHGYSFANITATLIAVVAAIIVYVISMLLIGGLAKDDII
                     MMPKGEKIAKKLAKYGFIE"
BASE COUNT          489 a          439 c          393 g          484 t
ORIGIN      
        1 ccagttttgc agcacctcac cggaatcctt cacagtcaca tcgccgaaaa cgtgcggatg
       61 acgctctatc agctttttgc agacaccgtc cgcaacatcg gagaaatcaa acgagccgtt
      121 ttccctctcc atctccgcat ggaacacgac ctgcattaaa acatcgccga gctcctcgca
      181 caaaagctcc ttatcctttt tgtcgatagc ctcaacaacc tcataggtct cttcgataaa
      241 gttctttttt atcgactcat gagtctgctc catatcccaa ggacagccgc ccggttcacg
      301 gagtatgcgc ataatgctta aaaggtcatc aacattgtat tttgatttaa actcaaaatc
      361 aaccgccatt tttaataagc cttcctttgt attttactct ataaatccat attttgcaag
      421 ctttttggca attttttctc ccttgggcat cataattata tcgtcctttg caagccctcc
      481 gataagcagc atggaaataa catagacaat tatagctgca actacggcaa tcaatgttgc
      541 ggttatattg gcaaacgaat atccatgtaa aagtctctga ggcaatatct tataggatat
      601 gccatatgca cagtatgcag ccacgccgca aagaaccgaa ctgataagcg gctttaccac
      661 aaccgacata aggttgactt tgaccttagt cgccttaata agtgctatat agttatatat
      721 taccacaatg agatagcaca aaacggtacc tatgaccgct ccgttgatat tgatggatgg
      781 tatgcctatg aaaatgtagt ttgccactac cttgacaacg gctccgacag tcagcgattt
      841 aacggggacg tttgctttgc cgacagcctg aagcatatta gtcatgggct gggaaattgc
      901 catcaggaaa atggtgtagc cgtaagccgc cattataggc gcggctattg atatggcggg
      961 tgcggatttg ccgttctcgt aaaacatacg aagtatcggc tccgccaaaa cgcccatacc
     1021 tataccggag gcaagcgata ttatcatacc cactcttaaa acggactcta ccgacgactt
     1081 cagcgcgcgc ttatctttaa cggcataagc cgccgagagt gccggtatag cgctgacgcc
     1141 gagagttgtt gttatggacg gaataaggtt tttaaagtca agagagagcg tatatgcacc
     1201 gtaaaggaaa tccttaaggt cggcgtcaag aacgtgagcc tcggctatct gcgtggcata
     1261 tatactcttt ataaggtcaa ggttatttga aataacgccg tcaagcctgt tttgtatggt
     1321 tatggcgtct ataagatttg taagactgaa aattatggac gacgtaacta cgggaatcgc
     1381 aatcgccaca agcgtttttg caatggctcc gctgtttacc ggacgcggcg aattgactat
     1441 ttcggtgcgc gttatgccgt cgcccttcgc cttgtgcatg attatcatgt atatcatacc
     1501 gataacggta cccatggtaa cgcctacggc cgctgccgct gccgcataag ggtaaatggc
     1561 actcatagcc tcggattcgg tcgcgcatac ctttccgaac accttagcac ccgactcaaa
     1621 ctgcgaataa ccgtatgtta tggtcagctt tgccgcagca ataccgaata tcattttgcc
     1681 tattgcctct acaacctgcg agatggctgt ggggttcata ttgcgaagtc cgttatagta
     1741 tccgcgatat attgacatca tacagcagaa gaatatggac ggagttatgg caataatggc
     1801 gggga
//
LOCUS       DBMDVO010000262         1804 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31292_length_1804_cov_1.254844, whole genome shotgun sequence.
ACCESSION   DBMDVO010000262 DBMDVO010000000
VERSION     DBMDVO010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31292_length_1804_cov_1.254844"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="ACZPE2_04460"
     CDS             <1..400
                     /locus_tag="ACZPE2_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024122.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIR1319134.1"
                     /translation="IHKDITEFWDEHEKKIKNFYAKIQAPDDVIVSACPEIILEEICK
                     RIGVKNYSGSVVDMENGIIERVCYKENKIAAFKSIYGDMPIDSFYTDSMSDKPMMDLA
                     NNAYLVSGDHIELVKNDGVWLSEPKTGLFK"
     gene            complement(453..1706)
                     /gene="hflX"
                     /locus_tag="ACZPE2_04465"
     CDS             complement(453..1706)
                     /gene="hflX"
                     /locus_tag="ACZPE2_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1319135.1"
                     /translation="MEFYENSQSPERALLIGVDTGEYNAEISMEELALLSETAGAEII
                     GTVIQKLEAPSPATYIGSGRMSEVAAFCRNNDIDLIIADSELTPSQINNIETKTDTRV
                     VDRTMLILDIFAARARSSEGKLQVELAQLKYSLPFLSGRGKSMSRLGGGIGTRGPGES
                     KLESDKRHIRRRIHSLSEELKRVEKRRDLIRSRRKKDGVITVAIVGYTNAGKSTLMNT
                     LTSAGVLSEDKLFATLDPTARRLTLPSKREVMLVDTVGLVRRLPHQLVDAFKSTLEEA
                     KNADLRLNVCDASSDECAEHLSVTSSVLKELGADNIPVISVMNKCDKLQNAYDMPVIG
                     KTVMISAVKNEGIDRLLEVIEDSLPKTKARAILLVPFSEGHILGEIRKTGVVYSESYK
                     ENGTRLDALVDIDYLEKIKRYIVSM"
BASE COUNT          458 a          451 c          388 g          507 t
ORIGIN      
        1 cattcacaag gacatcacag agttttggga cgagcacgaa aagaaaatca aaaacttcta
       61 tgccaagatt caagctcccg acgacgtaat agtttcggcc tgccccgaaa tcatactcga
      121 ggaaatatgc aaaagaatcg gcgtaaaaaa ctacagcggc agcgttgtgg atatggaaaa
      181 cggaatcata gagcgtgttt gctacaagga aaacaagata gcggcattta aaagcattta
      241 cggcgacatg cctattgaca gtttctacac cgattccatg agtgacaaac cgatgatgga
      301 cctcgcaaat aacgcttatt tggtttcggg cgaccacata gagcttgtaa agaatgacgg
      361 cgtatggttg agcgagccga aaacgggctt gttcaaataa taagttaagc aaaaagcagt
      421 aaatcagctt tgatttactg ctttttatgt ttttacattg acactatata acgcttgatt
      481 ttttcgagat aatcaatatc gacaagggcg tccagccgcg ttccgttctc cttatagctt
      541 tcggaataca cgactcccgt ttttcgaatt tctccgagta tatgtccctc gctgaacggg
      601 actaacaata tcgctctcgc cttggttttc ggcagcgagt cctcaataac ctcaagcagc
      661 ctgtcgattc cctcgttttt aactgcgctt atcataacgg ttttgccgat taccggcata
      721 tcgtatgcgt tttggagctt gtcgcactta ttcattaccg atataaccgg tatattatcc
      781 gctccgagct cctttaagac ggacgaagta acgctcagat gctcggcaca ctcgtcgctt
      841 gacgcatcac agacgtttag tctaaggtcg gcgttttttg cctcttcgag agttgatttg
      901 aaagcgtcga caagctgatg cggcaaacgc cttacaagcc ctaccgtatc aacgagcata
      961 acctctcgct ttgacggcag cgtaagcctt cttgcagtcg ggtcgagcgt agcaaaaagc
     1021 ttgtcctccg acagcacacc cgcacttgta agagtgttca taagagttga ttttccggca
     1081 ttggtgtaac cgactatggc aacggtgatt acgccgtcct tttttcgcct tgaacgaatg
     1141 aggtcgcgtc gcttttcaac cctcttaagc tcctcggaca acgaatgtat cctgcgtctt
     1201 atatggcgtt tatcgctctc gagcttgctc tcgcccggac ctcttgtgcc gattccgccg
     1261 ccgagacgcg acatactctt gcctctgccc gacaaaaacg gaagcgaata tttaagctgt
     1321 gccagctcca cctgcaattt accctcgctc gaccttgccc ttgcggcaaa aatatcgagt
     1381 atgagcatgg ttctgtcaac aactcttgta tcggtttttg tctctatatt gttaatttgc
     1441 gacggtgtaa gctcgctgtc ggctattata aggtctatat cgttattccg acaaaaggcg
     1501 gcaacctccg acattcttcc gctgccgata tatgttgccg gcgacggagc ttcaagcttt
     1561 tgtatcaccg ttccgattat ctcggctcct gcggtttccg agagaagagc caattcctcc
     1621 atagaaattt cagcgttgta ttcgcccgtg tccacaccta tcaaaagcgc tctttcggga
     1681 ctttgactgt tttcataaaa ctccataatt cgcctttaca gtttgttctg atttaaaaat
     1741 gacagcgcct ttataaatcc ggcgttgcac ttcgcataat aatacttata agaatcgcag
     1801 tagt
//
LOCUS       DBMDVO010000263         1803 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31320_length_1803_cov_1.275558, whole genome shotgun sequence.
ACCESSION   DBMDVO010000263 DBMDVO010000000
VERSION     DBMDVO010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31320_length_1803_cov_1.275558"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="ACZPE2_04470"
     CDS             <1..573
                     /locus_tag="ACZPE2_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014115971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1319136.1"
                     /translation="DGIMPAPRGVPQIEVTFDIDANGIVHVSAKDLGTQKEQSISITS
                     STNMSKDDIDKAVKEAAQFEEQDKKRKEEIDVRNEADQMVYQCDKILSENGDKINESD
                     KKDVEDKLNALKEALKGDNADDIKAKKEELTQSFYKISEEIYKQAAAAQQAAGGAQQN
                     PGAGAQGNAPGQDGYYETNYTDVEDDPNKK"
     gene            684..>1803
                     /gene="dnaJ"
                     /locus_tag="ACZPE2_04475"
     CDS             684..>1803
                     /gene="dnaJ"
                     /locus_tag="ACZPE2_04475"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR1319137.1"
                     /translation="MAEKRDYYEVLGVSKNATEDEIKKAYRQTAKKYHPDLNPGNKEA
                     EEKFKEANEAYEVLSDPQKKARYDQFGHAGVDPNYGAGAQGGAYGGAGFDFDLGDIFS
                     NIFGGGFGGGFGGGNSNPNAPQRGTDTQATVTVSFEEAAKGCKRDVTYSRIEVCDECH
                     GSGAAPGTSPKTCPDCHGKGQITSSQRTPFGMFQTQKACPKCNGKGTIIDSPCKKCHG
                     AGRIRKTATLTVDIPAGIDDRQIINARGQGNKGANGGPAGDLRIAVNVRPHPIFERDG
                     YNVWVEMHISFPAAALGCELQVPTLDGKVKYNVPAGTQSGTVFKLKNRGIQSLNGRGH
                     GDELVRVIVDVPRELTSEQKELMKQLDASFGNPANYGKE"
BASE COUNT          517 a          438 c          484 g          364 t
ORIGIN      
        1 gacggcatta tgcccgctcc ccgcggcgta cctcagatcg aggttacatt tgatatcgac
       61 gcaaacggta tagttcacgt ttcggctaag gacctcggca ctcagaagga acagtccatt
      121 tccataacct cctctaccaa tatgtccaag gacgatattg ataaggcggt taaagaagct
      181 gctcagtttg aggagcagga taagaagcgc aaagaggaga tcgatgtacg caacgaagcc
      241 gatcagatgg tttatcagtg cgacaagatt ctttccgaaa acggcgataa gataaacgaa
      301 tccgacaaaa aggacgttga agataagctc aacgctctta aagaggcctt gaagggcgac
      361 aatgccgatg acatcaaggc taagaaagaa gagcttactc agagcttcta caagatttcg
      421 gaggaaatct acaagcaggc tgctgcggct cagcaggcag caggcggtgc tcagcagaac
      481 ccgggtgccg gcgcacaggg caatgctccc ggtcaggacg gctattacga gacaaattac
      541 accgacgttg aggacgatcc caacaagaaa taatcacttt tatccgcttt ctctcctgcc
      601 cttttttggg gagaaggcgg taatgtgtta tttcaaatat actataaaca gcggcgccga
      661 cggcgccacg gagtgattgt tatatggcag aaaaaagaga ttattacgaa gtcctcggcg
      721 taagtaaaaa cgccaccgaa gatgagataa aaaaagcata tcgccaaact gctaaaaaat
      781 atcatcccga cttaaacccc ggcaacaaag aggccgagga aaagttcaag gaggctaacg
      841 aggcatacga ggttctctcc gaccctcaga aaaaagcgcg ttatgaccag ttcggccatg
      901 caggcgtaga cccgaattac ggggcaggag cgcagggagg agcatacggc ggagcaggct
      961 ttgattttga cctcggcgat atattcagca atattttcgg cggagggttc ggcggcggct
     1021 tcggcggagg caattcaaac cccaacgcac ctcagcgcgg taccgatact caggctactg
     1081 ttaccgtatc gtttgaagag gcggcaaagg gctgtaagcg tgatgtcaca tacagcagaa
     1141 tagaggtctg cgacgaatgc cacggcagcg gtgccgctcc cggtacttct cccaaaacgt
     1201 gcccggattg tcacggcaaa ggtcagatta catcttcgca gagaactccg ttcggtatgt
     1261 ttcagactca aaaagcctgt ccgaagtgta acggaaaagg tacgattatt gactcgccct
     1321 gcaaaaaatg tcacggcgcg ggaagaattc gtaagaccgc aacgctcacc gttgatattc
     1381 cggcgggtat tgacgaccgt cagattataa atgcgcgcgg tcagggcaac aagggcgcaa
     1441 acggcggccc agcaggcgat cttcgcatcg cggtaaacgt acgtcctcac ccgatattcg
     1501 agcgtgacgg ctacaatgtt tgggttgaga tgcatataag cttccctgcg gcagctctcg
     1561 gctgcgagct tcaggttccg actcttgacg gcaaggttaa atacaacgta cccgcaggca
     1621 ctcagagcgg aacggtattt aaacttaaaa accgcggtat ccagagcctt aacggcagag
     1681 gtcacggcga cgagcttgtc agagttatcg ttgatgttcc gcgagagttg acttcggagc
     1741 aaaaagagct tatgaaacaa cttgacgctt ctttcggaaa tcccgcaaat tacggtaagg
     1801 aag
//
LOCUS       DBMDVO010000264         1801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31379_length_1801_cov_1.014706, whole genome shotgun sequence.
ACCESSION   DBMDVO010000264 DBMDVO010000000
VERSION     DBMDVO010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31379_length_1801_cov_1.014706"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="ACZPE2_04480"
     CDS             <1..304
                     /locus_tag="ACZPE2_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1319138.1"
                     /translation="PDCEESDFYELDIGTAGIMRNYMAKKCDKYFTLEKKIERFLSLS
                     LRVYNVPQDEIKIIISRILEMDICSMANDVMQKLFKGLEMKFNFTLTEMGELQNEV"
     gene            336..>1801
                     /locus_tag="ACZPE2_04485"
     CDS             336..>1801
                     /locus_tag="ACZPE2_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319139.1"
                     /translation="MLASIIPSTAFAAETNVVDSDDTEQEQKDEEQEEKEIKEEKEEQ
                     EKQEEEKEEDSEESEQEEEQEESEKEEEEELPFTEDELALLEEDPEDLSLDEQATYFM
                     LIEYLDSIDGGIALYDGENANATKTVFKISTADQLVDLAGKVENDTSYWGGLWKDASF
                     EQVADINMKNIDWKGIGSFRGKYNGGGYSVKNIKIKANGTSAGFFSVAEGAVIENLAI
                     VNFGVDTSADYVGAVVGYATDTVIRNCWTSGSVTAKENAGGIAGYMDESGSVSKCYSL
                     CNVKSDNEAGGIVGNASGITITGAAVLGEKLEGSSVNRINGGNTAPDNCYAWGGMNAN
                     GNIQGMDITYNSGGFNPEFSDIFEDDDAWNFAEDLPVLADGNDEPGSNFPTYLYTSDG
                     DFKGSGTQSDPYLINNKYDLINLRNKVNGGENFTNKYFKQTADIDLEGENWIPIGNAN
                     LPAEGKYFNGIYDGGSHVIKNMKIIEYSRFTGLFGIIG"
BASE COUNT          593 a          326 c          460 g          422 t
ORIGIN      
        1 gccggactgc gaagaaagcg atttttatga gctggacatc ggcacagccg gtataatgcg
       61 gaattatatg gcaaaaaaat gcgataagta ctttacgctt gaaaaaaaga ttgagcgttt
      121 tttgtccttg agccttcggg tttacaatgt gccgcaggac gagataaaaa taattatcag
      181 ccgtattctt gaaatggaca tatgctcaat ggcaaatgat gtcatgcaga agctttttaa
      241 aggccttgaa atgaagttta attttactct taccgaaatg ggggaattgc agaatgaagt
      301 ttaaaaaatt attaagtata tttacagccg tgtgtatgct tgcgtcaatt ataccttcga
      361 ccgcatttgc ggctgaaacc aacgttgtcg attccgatga caccgaacag gagcagaagg
      421 acgaagaaca ggaagaaaaa gaaataaaag aagagaagga agaacaagag aaacaggaag
      481 aagaaaaaga agaggattcc gaagaatccg aacaggagga ggaacaggaa gaatccgaga
      541 aagaggaaga agaggagctt ccttttaccg aggatgagct tgcacttctg gaggaagatc
      601 ccgaagacct ctctcttgac gaacaggcaa cgtatttcat gcttattgaa tatctcgaca
      661 gcattgacgg cggtatcgcc ctttatgacg gtgaaaacgc aaacgcaaca aagactgtgt
      721 tcaaaatttc aacggcagac cagcttgttg atctggcagg caaggtcgaa aacgacacaa
      781 gctattgggg cggcttatgg aaggacgcaa gctttgagca ggttgccgat ataaatatga
      841 aaaatataga ttggaagggc ataggctcct tcagaggaaa atataacggc ggcggctatt
      901 ccgtaaaaaa tattaaaatc aaagcaaacg gcacatcggc aggcttcttt tccgttgccg
      961 aaggtgcggt tattgaaaat cttgcgattg tcaatttcgg cgttgatact tcggcggatt
     1021 atgtcggtgc ggttgtcgga tatgcaaccg atacggtgat aagaaactgc tggacatcgg
     1081 gcagtgtaac cgcaaaggaa aacgcaggcg gtattgccgg atacatggac gaaagcggct
     1141 ctgtaagtaa atgctattcc ttatgtaatg ttaaatccga taatgaggcc ggtggtattg
     1201 tcggaaatgc aagcggaata acgataacag gtgcggctgt tctcggcgaa aagctcgaag
     1261 gaagttccgt aaaccgcatt aacggcggaa atactgctcc ggataactgc tacgcatggg
     1321 gcggaatgaa tgcgaatggc aacatacagg gaatggatat tacatataat tccggcggct
     1381 tcaacccaga attcagcgat attttcgaag acgacgacgc atggaatttt gctgaggatt
     1441 tgccggtgct tgcggacggc aatgatgagc cgggcagtaa tttcccgaca tatctctata
     1501 cctcggacgg cgatttcaag ggcagtggca cacagagcga tccgtattta atcaacaata
     1561 aatatgacct cataaacctc agaaataagg taaacggcgg cgagaatttt acaaataaat
     1621 atttcaaaca gaccgccgat attgaccttg aaggagaaaa ttggattccg ataggcaatg
     1681 caaatctgcc ggctgaagga aagtatttta acggaattta tgatggcggc agccatgtaa
     1741 taaaaaatat gaaaataatc gaatacagta gatttacagg tctttttggt ataataggat
     1801 t
//
LOCUS       DBMDVO010000265         1800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31408_length_1800_cov_1.034138, whole genome shotgun sequence.
ACCESSION   DBMDVO010000265 DBMDVO010000000
VERSION     DBMDVO010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31408_length_1800_cov_1.034138"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="ACZPE2_04490"
     CDS             <1..429
                     /locus_tag="ACZPE2_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319140.1"
                     /translation="FYASLFLIGLQFAAVTLMPMIMTADCVDYYEFETGKRMEGAAYS
                     ILTLTIKVCLALGTALGLVFIQVSGYSDTVAQIAAGTASGFSEKTKDIVFFAYTVVPG
                     FLALISIFPIFKYDIVGKKKQDIADELQKRRTKATAEIDN"
     gene            complement(483..983)
                     /locus_tag="ACZPE2_04495"
     CDS             complement(483..983)
                     /locus_tag="ACZPE2_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319141.1"
                     /translation="MKLIVVSEDFNSRLSGELSQLLSKHGGVLLLGNNIIEASYIDTP
                     EYVITNIGAKLDNSFKNTISVEISKNGDIINEALIYNDSAGIKQRLNIGTDFKDDITL
                     SSLNDGEIYFGIQTIIKDLSGKNIYPCEIKSKTSDITDIHTLLTAAGILLISGKYHEN
                     NGILNI"
     gene            1151..>1800
                     /gene="hprK"
                     /locus_tag="ACZPE2_04500"
     CDS             1151..>1800
                     /gene="hprK"
                     /locus_tag="ACZPE2_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1319142.1"
                     /translation="MASKYSVSLDKLIKEFGFETLYLPEDAHDLSVTSQDVNRPGLIL
                     AGRDDYFDPERVQFLGLSEIEFLKTLSDEMRYESLKRLVETQPPFILITRNLEIPSHL
                     SSLVKEYKVPLLRTGESTSSCMSALISFLGTELAERITRHGVLVEVYGEGVLIVGDSG
                     VGKSETAIELIKRGHRLIADDAVEIRKVSSKTLVGSAPDNIRHFIELRGVGIINARR"
BASE COUNT          512 a          335 c          376 g          577 t
ORIGIN      
        1 ttctatgcat cgctgttttt aatcggcctt cagtttgccg ctgttacgct tatgcccatg
       61 ataatgacag ccgattgtgt tgattattat gagtttgaga ccggcaaacg catggagggc
      121 gcagcatact ctattttaac ccttaccata aaggtttgcc tagcactcgg aactgctcta
      181 ggccttgtgt ttatacaggt atcgggctat tcggatactg ttgctcaaat tgctgccggt
      241 actgcaagcg gatttagtga gaaaacaaaa gacattgtgt tttttgctta taccgtggtg
      301 cccggctttc ttgctctcat ttctatattt ccgattttta aatatgatat agtcggaaag
      361 aaaaaacaag atatagccga tgagctgcaa aaaagaagaa caaaagcaac tgcggaaatc
      421 gataattgag atatattgat cttagggctg taccgttgaa aaagcggtac agccttttgt
      481 ttctatatat ttaaaatccc attgttttca tgatattttc cggaaatcag aagaattccg
      541 gccgcagtta gaagagtgtg aatgtcggta atatctgatg ttttggattt tatttcacac
      601 ggatatatat ttttcccgga taaatccttg ataatcgttt gtattccgaa gtaaatttct
      661 ccgtcattta agcttgacag tgttatatcg tctttgaaat ctgtcccaat gttcagcctt
      721 tgctttatcc cggcagaatc attatatatt aacgcttcgt taatgatatc gccgtttttt
      781 gatatttcca cggaaattgt atttttgaaa ctgttgtcaa gttttgcacc gatgtttgta
      841 attacatatt cgggtgtatc aatatagctt gcctctatta tattatttcc gagcagcaat
      901 acgcctccgt gcttgctcag cagctgagaa agttcacctg ataaacggct gttaaaatcc
      961 tcggagacta caataagctt cactttatca ccccgaaata ttattttcaa ataacataag
     1021 gttattcaat ggttagaaac gtttaaaata ctagctcaaa atattaaaaa aatgctaaaa
     1081 agctattgga aagtctgtaa taatagatta taatattaca ttataacagt taaataaggg
     1141 aggctatggg ttggcttcaa agtattccgt ttcattagat aaactgataa aagaattcgg
     1201 ctttgaaaca ttgtatttgc cggaagacgc acacgaccta tccgttacat cgcaagatgt
     1261 taataggccg ggtcttattc ttgccggacg tgatgattat tttgacccgg aacgtgtgca
     1321 gtttttgggg ctttcggaaa tagaatttct taaaactcta agcgatgaaa tgcgttatga
     1381 aagccttaaa cggcttgtcg aaactcaacc gccgtttatt ttaattacac gaaatttaga
     1441 gatcccgtcg catctttctt cacttgtaaa agaatacaag gtgccgctct tacgtaccgg
     1501 tgaatccaca tcgagctgta tgtctgcgct tatttctttt ttgggaacag aacttgccga
     1561 aaggataaca cgacatggag tgcttgtaga agtgtacggc gagggcgtgc tgattgtcgg
     1621 cgacagcggc gtaggtaaaa gtgagactgc gattgaactt atcaagaggg ggcaccgtct
     1681 gattgcggat gatgccgttg aaattcgtaa ggtttcttca aaaacacttg tcggttctgc
     1741 accggacaat atcagacatt tcatcgaact tcgcggtgta ggaattatta atgctcgccg
//
LOCUS       DBMDVO010000266         1791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31618_length_1791_cov_1.353254, whole genome shotgun sequence.
ACCESSION   DBMDVO010000266 DBMDVO010000000
VERSION     DBMDVO010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31618_length_1791_cov_1.353254"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(172..>1791)
                     /locus_tag="ACZPE2_04505"
     CDS             complement(172..>1791)
                     /locus_tag="ACZPE2_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR1319143.1"
                     /translation="PQVILNQLYSYTQLQISYGIIQLAIVDGEPKILTLNQILEKYID
                     FQKEVIIRRTKFDLKKALEREHILEGLARAIDIVDEIIATIRACKGGQAEAKAAIMEK
                     FDFDDVQAAAIVAFRLGQLAGLEIQKIHNEMEELQSKINYFNEILSSDEKVLSIVKDE
                     LSAIGNKFGDDRRTEIVNVSGEVDIEDLIPNENCVFTLTTLGYIKRQSVDTYQTQHRG
                     GRGVKGMTRREEDVAETMFTCSTHDYIMFFTSEGKVYRLKGYEIPEGSRNSKGMNIVN
                     ILPLGADEKVTAMIRVPEFGTEKLYLCMLTKNGVIKRTELDAYKNVRKNGIFAINLDE
                     GDELRYVKLTDGEKQILIATRKGMCISFNENDARCIGRTARGVKAITLSEGDEIAGMC
                     VPEENKKVLTVSETGFGRRSEFDNYRVQSRGGKGLINYHTETYGDVAAVAMVGEDEDV
                     IMISSDGIIIRIPAKDISVFARPSKGVRVMKTNEGEKVATLSITEHETEDENSESDAE
                     NDGEVPVTKGNAEGEKAAEADESKENPTEIG"
BASE COUNT          393 a          450 c          399 g          549 t
ORIGIN      
        1 ccgggcggag gataattgcc gccgtaatat ccgttgttgt tatagttcat agcaatttcc
       61 ttttttcata acagaggttt tcggcagata aaaagcacct gccgaattac tctgtaatta
      121 tatattgtaa aagtaaatga aatgttaata aaattataac tttttgcgga attacccgat
      181 ttccgtcgga ttttctttgc tttcgtctgc ttctgccgcc ttttcgccct cggcatttcc
      241 cttcgtcacg gggacttcgc cgtcattctc cgcgtcggac tcggagtttt catcctcggt
      301 ttcatgctcg gttattgaaa gcgtggcaac cttttcaccc tcgtttgtct tcataacgcg
      361 gacgcccttt gacggacgtg caaaaacgct gatatcctta gccgggattc ttattatgat
      421 accgtcggac gaaatcataa ttacgtcctc gtcctcgccg accatggcaa ccgccgcaac
      481 atcaccgtag gtctcggtgt gatagttgat aagacccttg ccgccgcgcg actgaacacg
      541 gtaattgtca aattcggagc gtctgccgaa gccggtttcg gaaacggtga gtaccttttt
      601 gttttcctcg ggaacgcaca taccggcaat ctcgtctccc tcggagagag ttatagcctt
      661 aacgcctctt gcggttctgc ctatgcagcg cgcgtcattt tcgttaaagc ttatgcacat
      721 acccttcctt gtagctatga gtatctgctt ttcgccgtcg gtgagcttta catatctcaa
      781 ttcgtcgccc tcgtcgagat ttatcgcgaa tattccgttt ttgcggacgt tcttatatgc
      841 gtcaagctcg gtacgcttga taacgccgtt tttggtgagc atacagagat agagcttttc
      901 cgtaccgaat tcgggaactc ttatcatggc ggtgaccttt tcatccgcgc cgagaggaag
      961 aatattgaca atgttcattc cctttgaatt acggcttccc tcggggattt cgtagccctt
     1021 cagcctgtaa accttgccct ccgacgtaaa gaacattata tagtcgtgcg tggagcaggt
     1081 gaacatagtc tcggcaacgt cctcctcgcg ccttgtcata ccctttacgc ctctgccgcc
     1141 gcggtgctgc gtctgatatg tatcgacgct ctgacgcttg atatagccga gagtggtaag
     1201 agtaaacaca caattttcat tggggataag gtcctctata tcgacctcgc cgctgacatt
     1261 tacgatttcg gttcttctgt catcgccgaa tttgttgccg attgccgaca attcgtcctt
     1321 tacaatcgag agtacctttt catcggagga gagtatctca ttgaaatagt taatttttga
     1381 ctgaagctcc tccatctcgt tgtggatctt ctgaatttcg agtcctgcga gctgtccgag
     1441 tctgaaagcg acaatggcgg ctgcctgaac gtcgtcgaaa tcgaacttct ccataatcgc
     1501 agcctttgcc tctgcctgac cgcccttaca agcgcgaatg gttgctataa tctcgtcaac
     1561 tatatcgatt gcgcgcgcaa gaccctccaa aatatgctct ctttcgagtg cttttttgag
     1621 gtcgaatttg gttcttctta tgatgacttc cttctggaaa tctatatatt tttcgagaat
     1681 ctgatttaac gtgaggattt tcggctcgcc gtcaactatg gcaagctgaa ttataccgta
     1741 ggaaatctga agctgagtat acgaatagag ctgatttaaa atgacctgcg g
//
LOCUS       DBMDVO010000267         1789 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31673_length_1789_cov_1.319313, whole genome shotgun sequence.
ACCESSION   DBMDVO010000267 DBMDVO010000000
VERSION     DBMDVO010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31673_length_1789_cov_1.319313"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="ACZPE2_04510"
     CDS             complement(<1..1576)
                     /locus_tag="ACZPE2_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1319144.1"
                     /translation="MKKIELLAMLGESKDIIDYLQRKGTVQVEKCDAPESMYYTDTDS
                     SVSQLERLLQTAENARETISFYSPEKKKLTDSFLPRKEVELSDFYEESEKTDVAMAKC
                     REIIDIKKEIADKKADIIREQTAREALIPWERLDIPPNTTGTRHTEVFVGSLPDECTE
                     EALKAGFAAALPDLTALDCEVVSSSNTQSIIVVICLKKDSRRVYDYLRSLNFVRASGG
                     DNENIKAAMERHGKNIEALSSDIDGDEEKIRELSSYREDIDFLIDFLTIRKDKYEAFK
                     KMSLSRNVFYFEGYIPERYCEKTVKGLEERFTVAVTISDVPEDDENEPVLLKNNDFAA
                     PVEEITEMYALPGKHDIDPNAIMAFFYYCFFGMMFSDAGYGLIMFLATLFILLKCKPE
                     GQKRKTVMMYMYCGISTMFWGIMFGSFFGDIINIVRYDFIGLPKIRLYAWLDPQEQLM
                     TTMLWCFLFGVVHLFVGVGIKGYMEWRDGQRFWAVCDTLSVYLAVGGALPLCAGMIID
                     VPSDIKTVGKYCAIAGV"
BASE COUNT          438 a          490 c          360 g          501 t
ORIGIN      
        1 ttacgccggc tattgcgcaa tatttgccga ccgtttttat atccgacgga acgtctataa
       61 tcataccggc gcaaagcgga agcgcaccgc cgacagcaag gtaaacggac agcgtatcgc
      121 ataccgccca aaacctttgc ccgtcgcgcc actccatata gcccttgatg ccgacgccca
      181 caaaaagatg aacgacgccg aacaaaaagc accacagcat ggtggtcatc agctgctcct
      241 gcgggtcaag ccacgcatac aaccgtattt tcggcagtcc gatgaaatcg taccttacga
      301 tattgattat gtctccgaaa aagcttccga acataattcc ccaaaacatt gtggaaatac
      361 cgcagtacat atacatcata accgtttttc gcttttgccc ctcaggcttg catttaagca
      421 aaataaacag cgttgccaaa aacattatga gaccgtagcc cgcgtccgaa aacatcatac
      481 cgaagaagca gtaatagaaa aatgccataa tcgcattcgg gtctatatcg tgcttgcccg
      541 gaagagcgta catttcggtt atctcttcaa ccggtgcggc aaaatcatta tttttaagaa
      601 gcaccggttc gttttcgtcg tcctcgggaa catcgcttat cgttacggca acagtaaacc
      661 tctcttcaag gcccttaacc gttttttcac agtaccgctc cggaatgtag ccctcaaaat
      721 agaagacgtt gcggctgaga gacatctttt taaacgcctc gtatttgtct tttcttatcg
      781 tgaggaaatc aatgagaaag tctatatcct cacggtatga cgacagctct cttatttttt
      841 cctcgtcgcc gtcaatgtcc gacgaaagcg cttcgatatt ttttccgtgg cgttccattg
      901 ccgctttgat attctcgtta tcgccgcccg acgctctcac aaaattcaga cttcgcagat
      961 aatcgtatac tcgccttgag tcctttttca aacagatgac aacgattatg ctctgcgtat
     1021 ttgaagagga aacgacctcg cagtcgagag cggttaagtc cggcagagcc gcggcaaaac
     1081 cggccttgag agcctcctct gtgcactcgt ccgggagcga ccccacaaac acctccgtgt
     1141 gccttgtccc cgttgtattc ggcggaatat caagcctttc ccacggaatc agagcctctc
     1201 ttgcggtctg ttcgcggata atgtcggcct ttttgtcggc tatctccttt tttatgtcga
     1261 taatctcccg acactttgcc attgccacgt ccgtcttttc agattcctcg taaaaatcgg
     1321 acagctcgac ttcttttcgg ggcaaaaaag agtcagtaag cttctttttt tccggcgaat
     1381 aaaatgaaat tgtctctctt gcattctctg cggtttgcaa aagtctttca agctgtgata
     1441 cgctcgaatc ggtgtcggtg taatacatag actccggcgc gtcgcacttt tcaacctgaa
     1501 ccgtgccctt tcgctgcagg taatctataa tatccttgct ctcgccgagc atcgccaaaa
     1561 gctcgatttt tttcattttc aattttgccg taagccatca cctcactttg caaaatatta
     1621 tatcatttaa gtaatgtatc ctttacgatg ccgacagcct ccgaaagctt ttccgcagac
     1681 tgttttttca atccgtccgc gtacctttga gcctccttta cagcctcggt aacgataacc
     1741 tctgccgtat ctttggcttt tttgatttcg gcggcctcgt atacgacac
//
LOCUS       DBMDVO010000268         1786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31746_length_1786_cov_1.090208, whole genome shotgun sequence.
ACCESSION   DBMDVO010000268 DBMDVO010000000
VERSION     DBMDVO010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31746_length_1786_cov_1.090208"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="ACZPE2_04515"
     CDS             <1..502
                     /locus_tag="ACZPE2_04515"
                     /inference="COORDINATES: protein motif:HMM:NF028226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HIR1319145.1"
                     /translation="LGQPPKKEFVKQIEDEILSYPNIVGVHDLIIHDYGPGRQFASAH
                     AEVPSSVDVMKSHDTIDLIERNIQRKYNLLISIHLDPIVVNDAHINSLRDLTESAVKE
                     IDTALSIHDFRVVEGPTHSNLIFDVMAPPDFSLSDRELTNSIQEKLSKIDERYFCVIT
                     VDHSFN"
     gene            630..>1786
                     /locus_tag="ACZPE2_04520"
     CDS             630..>1786
                     /locus_tag="ACZPE2_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1319146.1"
                     /translation="MPDYTQEFTTIPYGPLGIIALPGCEELAQKIDRYLVKWRAQKQS
                     EHKNSLSFAGYERDTYIIDASFPRFGSGEGKGSLHESVRGYDIFIVCDMFNYGVTYNM
                     YGQTIPMSPDEHFANLKRAISAVGGKAHRITVIMPMLYEGRQHKRSSRESLDCAIALQ
                     ELCNNMGVDNIITFDAHDPRVQNAIPLKGFENVQPAYQMIKALVNNVDDLHLDKEHMM
                     VVSPDEGGMSRCIYYSTVLGLDLGMFYKRRDYSRVVNGRNPILQHQFLGSNIEGKDVM
                     VVDDMISSGESMLEVAKKLKELGAKRIFIFSAFGLFCEGLENFDKSFAEGNITKVFTT
                     NLVYRTPELLQRDWYCEVDMSKYLSYIIDTLNHDMSVSKLLNPADRIYALL"
BASE COUNT          507 a          426 c          371 g          482 t
ORIGIN      
        1 gctcggtcag ccgccgaaaa aggaatttgt gaagcagatt gaggacgaaa ttctctcata
       61 tcccaacata gtcggtgttc acgaccttat aatccacgac tacggccccg gcagacagtt
      121 tgcctcggct cacgcagagg ttccctcaag cgttgatgtt atgaagagcc acgataccat
      181 tgaccttatc gagcgaaata tccagagaaa atacaatctg ctcatttcaa tacaccttga
      241 ccctatcgtg gtaaacgacg cacatataaa cagtctgcgt gatttgaccg aatccgccgt
      301 caaggaaatt gacaccgcgc tttcaataca cgatttcaga gtggttgagg gtcccacaca
      361 ctcaaatctg attttcgatg taatggctcc cccggatttt agtctttccg atcgtgagct
      421 tacaaattca attcaagaaa aactaagcaa aatagacgaa agatatttct gtgtcattac
      481 tgttgaccac tcttttaact aatcttttat tttttgcctt ttttcccttg aaaatgacac
      541 aaaatatagt aaaatattat tatctaatgt aatgcggata aatggcgata atatttatcc
      601 gcaaaaattg tgtttcacgg aggaaaatta tgcctgatta cacacaggag ttcactacca
      661 tcccttacgg tccgctcggt atcatcgcgc tccccggctg tgaagaatta gcccaaaaaa
      721 tcgaccgtta tctcgtcaaa tggcgcgctc agaagcagtc tgagcacaaa aacagccttt
      781 cctttgcagg atacgaaaga gatacttata tcatcgatgc ctcattcccg agattcggct
      841 cgggcgaagg caagggctcg cttcacgagt ccgttcgcgg ttacgatatt tttattgtat
      901 gcgatatgtt caactacggc gtgacctaca atatgtacgg tcaaacaatt cccatgagtc
      961 cggacgagca ttttgccaac cttaaaagag cgatttccgc agtaggcggc aaagctcaca
     1021 gaataaccgt tattatgccc atgctttatg agggcagaca gcacaagcgt tcatcaagag
     1081 agtccctcga ctgtgcaata gctcttcagg agctctgcaa caatatgggc gttgacaaca
     1141 ttatcacatt tgacgctcac gacccgagag ttcagaacgc aattccgctc aagggctttg
     1201 aaaacgttca gcccgcttac cagatgataa aggctcttgt aaacaacgtt gatgatttgc
     1261 atctcgacaa agagcacatg atggtagtat ctcccgatga aggcggtatg tcacgctgca
     1321 tttactattc taccgttctc ggtcttgacc ttggtatgtt ctataagcgc cgcgactatt
     1381 cgagagttgt caacggcagg aacccaatac tccagcacca gttcctcggt tcaaacatag
     1441 agggcaagga tgtaatggtt gttgacgata tgatctcctc cggtgaatca atgcttgagg
     1501 ttgccaaaaa attaaaagaa ctcggcgcaa agcgtatttt tattttctcg gcattcggtc
     1561 ttttctgcga aggactcgaa aactttgaca aatcctttgc cgagggcaat atcacaaagg
     1621 tatttacaac aaaccttgtt taccgcacac ccgagctttt acagcgtgat tggtactgcg
     1681 aggttgatat gtctaagtat ctttcataca ttatcgacac cctcaaccac gatatgtcgg
     1741 taagcaagct cttgaatccg gctgacagaa tttatgctct ccttga
//
LOCUS       DBMDVO010000269         1785 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31783_length_1785_cov_0.993468, whole genome shotgun sequence.
ACCESSION   DBMDVO010000269 DBMDVO010000000
VERSION     DBMDVO010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31783_length_1785_cov_0.993468"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="nagB"
                     /locus_tag="ACZPE2_04525"
     CDS             <1..754
                     /gene="nagB"
                     /locus_tag="ACZPE2_04525"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1319147.1"
                     /translation="YEFKAYGVKNMEIKIYDNAEEIGKEVGSEFVKAVNAKPGIVLGL
                     ATGASPIPTYKYICEQYKNGLVSLENVKTFNLDEYVALPKNDKNSYYTFMHVQFFNNT
                     DIKEENVHFLNGNADDVDEECRAYDKLIKNAGGIDIQLLGVGRNGHIGFNEPSNHFTK
                     GSFKVKLTESTIEANSKYFDENPMPHYALTMGVGSIMSAKKIILIATGKAKADAVKAL
                     VDGEVSPACPASILQFHRDTVIYLDKDAASLL"
     gene            811..>1785
                     /locus_tag="ACZPE2_04530"
     CDS             811..>1785
                     /locus_tag="ACZPE2_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1319148.1"
                     /translation="MHNATNYGEVLSYNYKKNSIDFLPCGVIKTKIYVDVLVVINIFI
                     NYFLLSAASLIVRLKPKRIRLAAGAFTGGAYSLVIFLPNIPNVLSVFMNFCMSVLLVL
                     IAFAPKKITVFIKEFAAFFAVNFIFAGLMLAVWLVFKPSGMVYNNAAVYFDIDVKLLV
                     LSTVVCYAVLTLFSKLLKRNSPDDKLFDVKLCNKGAAVFTKALFDTGNLLSDGFSDTP
                     VIIAERSVVKKLVPEGIFLFIDGEAALPINADDGSIRLIPAGTVGGESILRAVLIDSV
                     TVIGKKEAREVKNVILAESKTPFSSGDYSVLLGPKIFEIREGNQKNERA"
BASE COUNT          507 a          334 c          420 g          524 t
ORIGIN      
        1 ttatgagttt aaagcatacg gagtgaaaaa tatggaaatc aaaatttatg acaatgccga
       61 agaaatagga aaagaggtcg gctcggaatt tgtaaaagcc gtcaatgcaa agccgggtat
      121 cgtactcgga cttgccacgg gagcgtctcc gataccgact tataaatata tttgcgagca
      181 gtataaaaac ggacttgtaa gtcttgaaaa tgtcaaaact tttaatcttg atgaatatgt
      241 ggctttgccc aaaaacgaca aaaacagcta ttacacattt atgcacgttc agttttttaa
      301 taatactgat ataaaagaag aaaatgtgca tttcctgaac ggcaacgccg atgatgtcga
      361 tgaggagtgc cgcgcctatg ataagcttat aaagaatgcc ggaggtattg atattcagct
      421 tctcggcgtc ggcagaaacg gtcatatagg atttaatgag ccgtcaaatc attttacaaa
      481 gggctcgttt aaggtaaagc ttacagagag caccattgag gcaaactcaa aatattttga
      541 cgaaaacccc atgccgcact atgcgcttac catgggcgtc ggttctatta tgagcgcgaa
      601 aaaaattata cttatagcta ccggaaaagc caaggcagac gccgtaaagg ctctcgttga
      661 cggtgaggtt tcgcccgctt gcccggcgtc gattcttcag ttccatcgcg ataccgtgat
      721 ttatctcgat aaggacgccg caagccttct ttaattaaaa tacaactata agctccgaag
      781 ctttgtgtgc ttcggagctt ttttgctgcc ttgcacaatg cgacaaatta cggagaggtt
      841 ttatcgtata attacaaaaa gaacagtatt gattttttgc cttgcggagt gattaaaacg
      901 aaaatatatg tagatgtgct tgttgtaata aatatcttta taaactattt tttgctgtcg
      961 gcggcctcgc tgattgtaag gctaaagccg aaaagaatac ggcttgcggc aggcgcgttt
     1021 acgggcggag catactctct tgtgattttt ttgcccaata ttccgaatgt actgtctgtt
     1081 tttatgaatt tctgtatgtc ggtgcttttg gttcttattg cctttgcgcc gaaaaaaatt
     1141 acggtgttca taaaagaatt tgccgctttt tttgccgtga attttatttt cgccggactt
     1201 atgcttgccg tctggctcgt ttttaagccg agcggtatgg tgtataacaa cgccgcggtc
     1261 tattttgata tagatgtgaa gcttctcgtt ttgtctacgg ttgtctgcta tgcggttttg
     1321 acgctttttt caaagctttt aaaacgaaat tcgcccgacg acaagctttt tgacgtaaaa
     1381 ctatgtaata aaggagcggc tgtttttaca aaggcgcttt ttgatacggg aaatcttctc
     1441 tccgacggct tctcggatac gcccgtaatt atagccgaac gctctgttgt gaaaaagctt
     1501 gtgccggagg gaatcttttt gtttattgac ggagaagccg ctctgccgat aaatgccgat
     1561 gacggaagca tcagactgat tccggcggga acggtaggcg gtgagagtat actgcgagcc
     1621 gtgcttatag attcggttac ggtaatagga aaaaaagaag caagagaagt aaaaaatgtc
     1681 attttggcgg agagtaagac tccgttttct tcaggtgatt attcggtgct tttagggccg
     1741 aaaatttttg agataagaga gggaaaccaa aaaaatgaac gcgct
//
LOCUS       DBMDVO010000270         1776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31962_length_1776_cov_2.035821, whole genome shotgun sequence.
ACCESSION   DBMDVO010000270 DBMDVO010000000
VERSION     DBMDVO010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31962_length_1776_cov_2.035821"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="rny"
                     /locus_tag="ACZPE2_04535"
     CDS             <1..818
                     /gene="rny"
                     /locus_tag="ACZPE2_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1319149.1"
                     /translation="PEAITLSCHDHVRREIARIALEKLIQDGRIHPTRIEETVEKARR
                     EVELKIKQEGERAVMETNVNGLHHELVRLIGRLYYRTSYRQNVLNHSIEVAHLAGIMA
                     SELGVDATLARRAGLLHDIGKALNHEIEGSHVQIGVDVCRKYKESPEVIHAIEAHHGD
                     VEIHSVIAALVQAADAISAARPAARSENYENYIKRLQKLEEICSSYNGVEKSFAIQAG
                     REVRIMIKPEQVDDDKMIVLAREISQRIENEMEYPGQIKVHLIRESRAIDYAK"
     gene            886..>1776
                     /locus_tag="ACZPE2_04540"
     CDS             886..>1776
                     /locus_tag="ACZPE2_04540"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR1319150.1"
                     /translation="MKKRLTAILTVMALILTGCAGNVTSGTESQPASDAQSGISDNES
                     SDTSSRYEIITATEMAEKMGLGISLGNTMEAYEATDCEKITYEWIPVVGGNEPQDYET
                     CWGADVTTQEIIDGMKAEGFNTVRIPVFWGNMMENDGTYTINKEYLKRVKEIVDYCEK
                     AGVYSVINIHHFDEFIIRRNDLDKCKEIFTHLWTQIAEYFADYPYTLVFEGYNEYLGG
                     NQFDSSGNLKEQSETNAYKMTNTLNQAFVDAVRGTGRYNAQRVLIVSGYWTNIDKTTS
                     SRFQMPEDMVNDRLMVSVHYV"
BASE COUNT          554 a          385 c          442 g          395 t
ORIGIN      
        1 cccccgaggc tataacgctt tcctgccatg accatgtaag acgtgagata gcaagaatag
       61 cgcttgaaaa gctcattcag gacggacgta tccatcccac gagaattgaa gaaacagtag
      121 aaaaggcaag acgtgaggtc gagcttaaaa tcaagcagga gggcgagcgt gccgttatgg
      181 aaaccaacgt aaacggcctg caccacgagc ttgtaaggct tatcggccgc ctttactacc
      241 gtacctctta ccgtcagaac gtacttaacc actcaataga ggtcgctcac ctcgcaggaa
      301 ttatggcaag cgagcttggc gttgacgcaa ctcttgcaag acgtgccggt cttctgcacg
      361 atataggcaa ggctcttaac cacgagattg aaggctcaca cgttcagata ggtgtcgatg
      421 tctgccgtaa gtataaggaa agtcccgaag ttatccacgc tatcgaggct caccacggcg
      481 atgttgagat acactctgtt attgccgctc ttgtacaggc agccgacgca ataagcgccg
      541 cacgtcccgc cgcaagaagc gaaaactacg aaaactacat caagcgtctg cagaagctcg
      601 aagagatatg cagctcttac aacggtgttg aaaagtcatt cgctattcag gcaggccgtg
      661 aagtaagaat catgataaag cccgaacagg ttgacgatga taagatgata gttcttgcaa
      721 gagaaatttc tcagcgtatc gagaacgaga tggaatatcc cggtcagata aaagtacatc
      781 tgataagaga aagcagagct atagattacg caaaataaac cataaacact tgggagccgt
      841 tgcatagatt tatgtaacgg ctctttttgc taaagaggta ttcttatgaa gaagagatta
      901 acggcaatac ttacggtaat ggcacttata ctgacaggct gtgccggaaa tgtcacatcg
      961 ggaacggaaa gtcagccggc gtcggacgct cagagcggta tttcggataa cgaaagcagt
     1021 gatacttcat cacgctatga gataatcaca gcaaccgaaa tggctgaaaa aatggggctt
     1081 ggcataagtc ttggcaacac gatggaagca tacgaagcga ccgattgcga aaagataacg
     1141 tatgaatgga ttcccgttgt cggcggaaac gaaccgcagg actatgaaac ctgctgggga
     1201 gcggatgtta ccacgcagga aataatagac ggtatgaagg cggaaggctt caacacagtt
     1261 cgtattcctg tgttctgggg caatatgatg gaaaatgacg gtacatatac gataaacaag
     1321 gaatacctta aaagagttaa ggagattgtt gattactgcg aaaaggcagg cgtatattcc
     1381 gttataaata tccaccactt cgacgaattt atcataagaa gaaacgacct tgacaagtgc
     1441 aaggagatat tcactcatct gtggacgcag atagccgagt attttgcgga ttatccctac
     1501 acactcgttt tcgagggcta taacgaatat ctcggcggca atcagtttga ttcaagcggt
     1561 aatctcaagg aacagtccga aaccaatgct tacaagatga ccaacacgct caatcaggcg
     1621 tttgtcgatg cggtaagagg cacaggcaga tataacgcac agcgtgtgct tatcgtttcg
     1681 ggctactgga caaacataga taaaacgact tcttcgagat ttcaaatgcc tgaggatatg
     1741 gtaaacgaca ggcttatggt gtctgtccac tatgtc
//
LOCUS       DBMDVO010000271         1776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31972_length_1776_cov_1.197612, whole genome shotgun sequence.
ACCESSION   DBMDVO010000271 DBMDVO010000000
VERSION     DBMDVO010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31972_length_1776_cov_1.197612"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="ACZPE2_04545"
     CDS             complement(<1..1701)
                     /locus_tag="ACZPE2_04545"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1319151.1"
                     /translation="MFFKNFKVWETEFAGRKLSVETGKMAGLANASVMVRYGDTNVLC
                     TVTASAKPREGVDFFPLSVDYEEKMYSVGRIPGSFQRREGRASEKAILTSRCIDRPIR
                     PLFPKDLRNDCSVVATVMSVDPDCSPEIAAMIGVSTAIAISDIPWDGPISGVNVGLVD
                     GEIVLNPTLEQRAKTDLNLTVASTADLVAMIEAGGNEISDDLMFDCIMAGHEENKKIV
                     KFIQGIVDEIGKPKFAYESSDPDPEIMKEIEDFCIEDVKKALDTDDKLVRDEALLPIY
                     EAVHEKFDERFEGNEGKIEEIMYLVQKHVVRSWLKDEHKRVDGRGIDEIRPLNAEIDL
                     LARAHGSGLFTRGQTQVLSVATLGSMSDCQQLDGLDEQKEKRYIHHYNFPSYSVGETK
                     PSRGPGRREIGHGALAEKALLPVIPSVDEFPYAIRVVSEVLSSNGSTSQGSVCGSTLA
                     LMAAGVPIKAPVAGISCGLITGDEGVYGDFMTMVDIQGLEDFYGDMDFKVAGTKKGIT
                     AIQMDLKVHGLTPAIIKEAFAKTHKARNYILDEIMLPVISEPRKELSKWAPKMLTTKI
                     D"
BASE COUNT          413 a          462 c          440 g          461 t
ORIGIN      
        1 atcaatcttg gtggtaagca tcttgggcgc ccactttgaa agctccttgc gcggctcgct
       61 tataacggga agcattattt cgtcaagtat ataatttctt gccttgtgag tctttgcaaa
      121 cgcctctttg atgattgcgg gagtaaggcc gtgaacctta agatccatct gaattgcggt
      181 tatacccttt ttggtacctg caaccttgaa gtccatatcg ccgtagaagt cctcaagacc
      241 ctgaatatca accatggtca taaagtcgcc gtaaacgccc tcgtcacctg tgataagtcc
      301 gcaggaaata cctgccacgg gagcctttat cggaacgcct gccgccataa gtgcaagtgt
      361 gctgccgcag acagagccct gtgaagtgga gccgtttgac gagagaacct cggatactac
      421 gcggattgca taggggaact catcaaccga cgggataacg ggaagaagag ccttctcggc
      481 aagtgcaccg tgacctatct cacgtctgcc ggggcctctt gaaggcttgg tctcgcctac
      541 cgagtaggac gggaagttgt agtgatgaat atatctcttt tccttctgct cgtcaaggcc
      601 gtcaagctgc tggcaatcgc tcatggagcc gagtgtggca accgagagaa cctgagtctg
      661 acctcttgtg aacaaacccg agccgtgcgc tcttgcaagg aggtcgattt cggcgttgag
      721 cggacgaatt tcgtctatgc ctctgccgtc gactctcttg tgctcgtcct tgagccagga
      781 gcgaacaacg tgcttctgaa caagatacat aatctcctcg atcttgccct cattgccctc
      841 gaatctctca tcaaatttct cgtgaacagc ctcgtatatc ggcaaaagag cctcgtcgcg
      901 gacaagcttg tcgtctgtat caagagcctt tttgacatcc tctatgcaga agtcctcaat
      961 ctccttcata atttcgggat cggggtcgga ggattcgtaa gcgaacttcg gcttgccgat
     1021 ttcgtctaca atgccctgaa tgaactttac gatttttttg ttttcctcgt gacctgccat
     1081 aatgcagtcg aacataaggt catcggaaat ttcgttgccg cctgcctcaa tcatggcaac
     1141 aaggtctgcc gttgaagcaa cggtaaggtt aaggtcggtc tttgctctct gctcaagagt
     1201 cgggttcaat acgatttcgc cgtcaacaag tcctacgtta acgccggaaa tcgggccgtc
     1261 ccacggaata tcggaaattg cgatagcggt tgaaacgcct atcatcgcgg caatttcggg
     1321 gctgcaatcg gggtcaaccg acataacggt tgcaaccacg gagcagtcat tgcggagatc
     1381 cttcgggaaa agcggacgaa tggggcggtc tatacatctt gatgtgagaa tcgccttttc
     1441 gcttgctctg ccctcgcgac gctggaatga gccggggatt ctgccgacgg aatacatctt
     1501 ctcctcgtaa tcaacggaga gcgggaagaa atcaacgccc tctctcggct ttgccgaagc
     1561 cgtaacggtg caaagcacgt ttgtgtcgcc gtagcgaacc attacagacg catttgcaag
     1621 tcctgccatt ttgccggtct caaccgagag ctttctgccg gcaaattcgg tttcccaaac
     1681 cttgaagttt ttgaaaaaca tcttttttac ctctttttct taaatttccg agaggcactt
     1741 ccggttttag caataaatgc gacctttaag aggaaa
//
LOCUS       DBMDVO010000272         1776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_31975_length_1776_cov_1.030448, whole genome shotgun sequence.
ACCESSION   DBMDVO010000272 DBMDVO010000000
VERSION     DBMDVO010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31975_length_1776_cov_1.030448"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="ACZPE2_04550"
     CDS             complement(<1..755)
                     /locus_tag="ACZPE2_04550"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1319152.1"
                     /translation="MSVKNDIRSELEAHRGEDISGQYLAEKLGVSRNAVWKAINSLKN
                     DGYKITSSTNKGYCLSESCDILSQSTIERLCGENTDIKIFESLDSTNNEAKRETATGK
                     TDKPLLIISEEQTLGRGRNGRNFYSPKGTGLYFSLVTSPKSDLTSAVGITSYAAVCVV
                     EAIKELTGTDTKIKWVNDIYLDGKKLCGILTEAVSDFETGTVSNIIIGIGINLKTATL
                     PDSLKDKVGFLEYDLPIKNELISLIVKKLLKYD"
     gene            complement(899..1162)
                     /locus_tag="ACZPE2_04555"
     CDS             complement(899..1162)
                     /locus_tag="ACZPE2_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319153.1"
                     /translation="MAEISKKIRQSKVLCGESKILCARAVVLMSLFTFTFLGAEFLFV
                     NIIARTASGNLSVNAQNYVLGIYGGALTLFIIVGKKKKAVLPK"
     gene            complement(1246..>1776)
                     /locus_tag="ACZPE2_04560"
     CDS             complement(1246..>1776)
                     /locus_tag="ACZPE2_04560"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1319154.1"
                     /translation="FGDITVNDGEMVYTSIPYDTNWEVYVDGVKVKADDMVKISDSLM
                     GFYAEPGEHEIYFRYYSYGLRNGAIISIITIIIATLLIIMKRRKVLFYKKNKQDKWLL
                     LNENAAAFDVSSAANPNDASISGHAAPPTEAIKPDLPEDNSTADKTPDEEISSPQKGS
                     DNAEKEKIKNDNNGEA"
BASE COUNT          415 a          384 c          374 g          603 t
ORIGIN      
        1 tcatcatact ttagtaattt tttaactata agcgaaatta actcgttttt aatcggtaaa
       61 tcgtattcca aaaaaccaac cttgtccttg agtgagtccg gcagggtggc ggtttttaga
      121 ttgattccta tgcctattat tatgttgctt acggtaccgg tttcaaagtc ggataccgct
      181 tccgtcagta ttccgcacag ctttttgccg tcgagatata tgtcgtttac ccatttgatt
      241 tttgtatcgg ttcccgtgag ctccttaatt gcttcaacta cgcagactgc ggcatatgac
      301 gttataccga cggctgacgt caaatcgctt ttaggagagg tgacgagcga aaaatataat
      361 cctgtaccct taggcgagta aaaattgcgt ccgtttctgc cgcgaccgag agtttgctcc
      421 tccgaaatga tgagcaacgg cttgtcggtt tttccggttg ccgtttctct ctttgcttca
      481 ttgttggtgg agtctaagct ctcaaatatt tttatgtccg tattttcgcc gcaaagcctt
      541 tcaatggtgc tttgcgagag aatatcgcag gactccgaca aacagtaccc cttatttgtg
      601 gaagatgtaa ttttatatcc gtcgttttta agtgaattta ttgctttcca tacggcgttt
      661 ctcgatacgc cgagcttttc ggcaagatat tgtccggaga tatcttctcc tctgtgtgct
      721 tcaagctccg agcgtatgtc attctttaca gacatagttt tttcctttca agaaataatc
      781 tgcatatatg gtattgtata aaacgcgttt tgtcaaattg ccttatgaaa tttttaaaaa
      841 gcggacagca actttgaatg tcgctgtccg cttttatatg gatttaggtg tttacccgtt
      901 atttaggcaa tactgccttt ttctttttgc cgacgatgat aaataaggtc agcgcgccgc
      961 cgtagatgcc gagcacatag ttttgcgcgt ttaccgacag attgcccgat gccgtgcgtg
     1021 cgattatatt gacaaacaaa aattcagcac ctaaaaacgt aaaggtgaaa aggctcatga
     1081 gcacaaccgc tcttgcgcag agaattttac tttccccgca caagaccttg ctctgtctga
     1141 tttttttcga aatctcagcc atattaaatg tcctctttgc ttatcggtcg tgattttttc
     1201 ggcttaagca aataaaaatc atattgtgca gggattaccc ctgctttaag cttcaccgtt
     1261 attgtcgttt tttattttct ctttttccgc attgtccgag cctttttggg gtgaactgat
     1321 ttcttcgtcc ggcgttttgt ctgcggtcga attgtcttct ggtaagtcgg gctttatggc
     1381 ttctgtcggc ggagccgcgt ggccgcttat tgaggcatca ttcggatttg cagccgaact
     1441 tacgtcaaaa gcggcggcgt tttcgtttaa caacaaccat ttatcctgtt tattcttttt
     1501 ataaaacaga actttgcggc gtttcattat tatgagcagc gttgctatga taatagtaat
     1561 aattgagatt attgcgccgt ttctgagtcc gtaggaataa tagcggaagt aaatttcgtg
     1621 ttctcccggt tcggcgtaaa agcccataag gctgtcggaa atcttaacca tatcatcggc
     1681 ttttacctta actccgtcaa catatacctc ccagttcgtg tcgtagggaa tagaggtata
     1741 gaccatttcg ccgtcattta cggttatgtc tccgaa
//
LOCUS       DBMDVO010000273         1775 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32003_length_1775_cov_1.044803, whole genome shotgun sequence.
ACCESSION   DBMDVO010000273 DBMDVO010000000
VERSION     DBMDVO010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32003_length_1775_cov_1.044803"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="ACZPE2_04565"
     CDS             <1..530
                     /locus_tag="ACZPE2_04565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR1319155.1"
                     /translation="FYSTEKFELEDKGSFWLSETPEVMSKDWGAACYRVCSYVILKEK
                     QTGKDFVVFNTHLDHISDEARINGIRVVLDKIAQFGGKPSIIMGDFNAKENSETYKMA
                     TENFNDAKYLTDNPDNSCTYQNFGKALDRSCIDYFMVSKTGITAQSFEIVKNTYDGVY
                     PSDHFPIAVTLTLTD"
     gene            559..1686
                     /locus_tag="ACZPE2_04570"
     CDS             559..1686
                     /locus_tag="ACZPE2_04570"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF026257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR1319156.1"
                     /translation="MTGKTLAREYLCSVVRSALLSEPLSDIPEGTDKVFLVKLAYRNS
                     VQVILYLAFKDKPGALPQEYMSKLERSYKAAILRESAQQNELNFIRKAFAENNIDFMC
                     LKGAHLKALYPVAEMRFMVDMDILVKKEDVLRAEKLLTERGFSREMNNGKDIVLINPP
                     FLTVELHNMLFIESDSRHDYFTDVWKRAVKCGEHEYKMTDNDLYIYVMAHLAEHYKDG
                     GACFRPTMDIFMLNRLKSEELDFTYIGGELEKIGLARFAENIKKVGDIWYGDAKDDKA
                     LFVMQQYIVLGPPIQNAGAVAENMESTRFSAFMRMAFPPLKVMVKNYPVLKRLPFLLP
                     FYWLVRLVKKGGRAKNKSKELGALTDKNKKLMNDIFKSSGL"
BASE COUNT          542 a          356 c          433 g          444 t
ORIGIN      
        1 tattttacag caccgaaaaa ttcgagcttg aggataaagg ctcattttgg ctttccgaaa
       61 cgcccgaggt tatgagtaag gattggggcg cagcgtgtta cagagtttgc agctatgtta
      121 tacttaaaga aaaacagaca ggtaaagatt ttgttgtgtt taacactcac cttgaccata
      181 tcagcgacga ggcgcgcata aacggaattc gcgttgtgct tgacaaaatt gcgcagttcg
      241 gaggcaagcc gtcgataatt atgggcgatt ttaatgctaa agagaacagc gaaacatata
      301 aaatggcaac cgaaaacttt aacgacgcaa agtaccttac ggataacccc gacaactcct
      361 gtacatatca gaatttcggc aaggcacttg acagaagctg tatagactat tttatggttt
      421 caaagacggg tattacggcg cagagctttg agattgtgaa aaacacatat gacggagtat
      481 atccgagcga ccatttcccg attgccgtta cacttacgct tacagactga taccgtattt
      541 aatgaaagga taaaaaagat gaccggaaaa acgcttgcaa gagaatatct ttgctccgtg
      601 gttcgttcgg ctctgctctc ggagccactg tccgatatac ccgagggcac cgataaggtt
      661 ttcctcgtga agctcgcata tcgcaacagc gttcaggtca tactctacct tgcttttaaa
      721 gataagccgg gggctttgcc gcaggaatat atgtcaaagc ttgagcgttc atataaggcg
      781 gcgattctcc gtgagagcgc acagcaaaac gagcttaatt tcataagaaa agcttttgcc
      841 gaaaacaata tagattttat gtgcctcaag ggagcacacc tcaaagcact ttatcctgtg
      901 gcggaaatgc gctttatggt ggatatggac attcttgtca aaaaagagga tgttttgcgc
      961 gcggaaaagc tgttgacgga acgcggcttt tctcgcgaaa tgaacaacgg caaggatata
     1021 gtccttataa atccgccgtt tttaaccgtg gagcttcaca atatgctctt tattgagagc
     1081 gacagtcggc acgactattt taccgacgtt tggaaacgag ccgtaaaatg cggcgagcac
     1141 gaatataaaa tgaccgacaa tgacctttat atatatgtta tggcacatct ggcggagcat
     1201 tacaaggacg gcggagcgtg cttcagaccg actatggata tttttatgct taaccgcctt
     1261 aaaagcgaag agcttgattt tacgtatata ggcggcgagc ttgaaaaaat cggacttgcg
     1321 cgctttgctg aaaacataaa aaaagtcggc gacatttggt acggcgatgc aaaggacgac
     1381 aaagctctgt tcgtaatgca gcagtatata gttttgggtc cgcccataca aaatgccggc
     1441 gcagtagccg aaaatatgga aagcacgcgc ttttcggcgt ttatgcgaat ggcgtttccg
     1501 ccgcttaagg ttatggtgaa aaactatccc gtgcttaaaa gactgccgtt tttgctgccg
     1561 ttttattggc tcgtcagact cgttaaaaag ggcggcaggg caaagaataa gtcaaaagag
     1621 cttggggcgt tgacggataa gaataagaag ctgatgaatg atatttttaa atcctcgggt
     1681 ctgtaatacc gaattcggag aggcggaaaa tgaaaaacaa acaaaaagta agcagcgata
     1741 aatttaccat gatgacgcaa aagccggtaa ataag
//
LOCUS       DBMDVO010000274         1769 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32147_length_1769_cov_1.215228, whole genome shotgun sequence.
ACCESSION   DBMDVO010000274 DBMDVO010000000
VERSION     DBMDVO010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32147_length_1769_cov_1.215228"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="ACZPE2_04575"
     CDS             complement(<1..487)
                     /locus_tag="ACZPE2_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024231.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1319157.1"
                     /translation="MVKKWLSYIFPCILVPAAVLLGATVFKDKQYAWISLCVALLSCV
                     PFFLAFEKGKSGTKEMILIAVMVALSAVGRLIFAWAPSVKPVTALVIITAMYFGPQAG
                     FMTGALTAVISNFYFGQGPWTPFQMFSWGIVGLIAGVFAPQLKKSLVFRIIYSAVSGV
                     LY"
     gene            complement(480..>695)
                     /locus_tag="ACZPE2_04580"
     CDS             complement(480..>695)
                     /locus_tag="ACZPE2_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319158.1"
                     /translation="THDVEFAAVGADRCALFFDGEIVSSDAPNSFFAENNFYTTAANR
                     IARPLCPTAITSEEAAECCRGNGEDNG"
     assembly_gap    696..705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<706..1751)
                     /locus_tag="ACZPE2_04585"
     CDS             complement(<706..1751)
                     /locus_tag="ACZPE2_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319159.1"
                     /translation="MASYFGIQDWFRKKTDELSGGQKQLLNLASVMVMQPKVLILDEP
                     TSQLDPIAAADFIATLKKLNNEFGITVLLVEHRLEDVFPIADKVLLMENGCVLLYDSP
                     RAVGKNLKKINPEHPMLKGLPSAVRLYNLLSSDEVCPLTVKEGQEFFRRNFKRNIDFS
                     DGDKSEYSDKTDTAIELKNVWFRYERDLPDILRGVNLKAVRGEIFCILGGNGTGKTTT
                     LNVISGLNKPYRGKIKIDGKKIKDYKGNSLYRKKLAYLPQNPQTVFLKDTVSGDLEEM
                     LKAMEYKKEEREEKIRDISEKLGITDLLTKHPYDLSGGEQQKCALAKMLLSEPAILLL
                     DEPTKGLDAFSKSSL"
BASE COUNT          472 a          458 c          370 g          459 t
ORIGIN      
        1 aatacagcac tcccgaaacg gcactgtaaa ttattctgaa cacaaggctc tttttaagct
       61 gcggagcgaa aactccggct ataagcccga ctatacccca cgaaaacatc tggaacggtg
      121 tccatggacc ctgcccaaaa taaaaattcg atattacggc ggtcagcgca cccgtcataa
      181 aacctgcctg tgggccgaaa tacatcgctg ttattataac gagtgccgtc accggtttta
      241 cgctgggtgc ccacgcaaaa ataagtctgc cgactgccga caacgcgacc ataacagcta
      301 ttaaaatcat ttcctttgta ccgctttttc ccttttcaaa ggcgagaaaa aacggaacgc
      361 aagacaacaa ggcaacgcaa agcgatatcc atgcatactg tttatcttta aacactgtgg
      421 caccgagcaa aaccgccgca ggcaccaaaa tgcacggaaa aatatacgaa agccattttt
      481 taaccattat cttctccgtt tccgcggcag cactcagccg cctcttcact cgttatcgct
      541 gtgggacaaa gcggacgggc tatgcggttc gccgccgtcg tataaaaatt attttctgcg
      601 aaaaacgaat tcggcgcatc ggaagaaacg atttctccgt caaagaacag cgcgcaccta
      661 tcggcaccga cagcggcaaa ctcaacatca tgagtnnnnn nnnnnagcga ggactttgaa
      721 aaggcgtcaa gtccctttgt aggctcgtcc aaaagcagta ttgcaggctc tgaaagcaac
      781 attttcgcaa gggcgcattt ttgctgttca ccgccgctga ggtcataagg atgctttgta
      841 agcaaatccg ttataccgag tttttcggaa atatcgcgta ttttttcttc gcgctcttct
      901 tttttatact ccatcgcctt gagcatttct tcgagatcac ccgaaacggt atcctttaaa
      961 aataccgtct gcggattttg cggcagatat gcaagcttct ttcggtacag cgaattgccc
     1021 ttataatcct tgattttttt gccgtctatc tttattttgc cgcggtacgg cttattaaga
     1081 cccgaaataa cattcagcgt tgtcgttttt cccgtgccgt tgccgccgag aatacaaaat
     1141 atctcgccgc ggactgcttt aagatttacg ccgcgcagaa tgtcgggcaa atccctttca
     1201 tatctgaacc aaacattttt aagctcgatt gccgtatcgg ttttatcgga atattccgat
     1261 ttatcaccgt cggaaaagtc tatattcctt ttgaaattac gtctgaaaaa ctcctgcccc
     1321 tctttaaccg taagcgggca gacttcgtcc gacgagagca gattgtaaag ccttacggca
     1381 ctcggaagac ccttgagcat agggtgctcg ggatttattt ttttaagatt tttgccgacg
     1441 gcacgcggtg aatcatacaa aagcacgcag ccgttttcca taagcaaaac cttgtcggct
     1501 atcgggaaaa cgtcctcaag acggtgctct accaaaagca cggtaatacc gaattcgtta
     1561 ttcagcttct taagcgtcgc tataaaatca gccgcggcta tggggtcaag ctgacttgtg
     1621 ggctcatcta aaataagcac cttgggctgc attaccataa ccgacgcaag atttaaaagc
     1681 tgcttctgac cgccagaaag ctcgtcggtt tttttgcgga accaatcctg tataccgaaa
     1741 tagctcgcca tctcacccac tctgcggcg
//
LOCUS       DBMDVO010000275         1766 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32219_length_1766_cov_1.072673, whole genome shotgun sequence.
ACCESSION   DBMDVO010000275 DBMDVO010000000
VERSION     DBMDVO010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32219_length_1766_cov_1.072673"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            222..887
                     /locus_tag="ACZPE2_04590"
     CDS             222..887
                     /locus_tag="ACZPE2_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1319160.1"
                     /translation="MILKEHIYKYGEKLPNENELSAKLGISRTTLREAIRILASDGLL
                     EVRRGLGTFVAQPLNQYAEGSIDFQEFSKLKITLRDLYETRMIFEPEAAALASRRATE
                     DEIKEILHLGDICQQELLRDPQGKRRIDSEAAFHGAILKASHNDFLSRFMPILTETIE
                     QTFALNFNLDMIAEQAYKDHILIMDFLEKRDAVALKSAVTIHLHHALLTEQISLEENE
                     KKY"
     gene            986..>1450
                     /locus_tag="ACZPE2_04595"
     CDS             986..>1450
                     /locus_tag="ACZPE2_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1319161.1"
                     /translation="MAEARMFYEKDCNLSLLDGKTIAVIGYGSQGHAHALNAKESGCN
                     VIIGLYEGSKSWAKAEQQGFEVYTAAEAAKKADIIMILINDELQADMYKKDIEPNLKE
                     GDMLMFAHGFNIHFGCIKPPKFVDVTMIAPKAPGHTVRSEYQAGKGTPCLVAV"
     assembly_gap    1451..1460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1461..>1766
                     /locus_tag="ACZPE2_04600"
     CDS             <1461..>1766
                     /locus_tag="ACZPE2_04600"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1319162.1"
                     /translation="LAIGGARAGLLETTFRTETETDLFGEQAVLCGGVCALMQAGFET
                     LCEAGYDPRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIVTED"
BASE COUNT          492 a          423 c          421 g          420 t
ORIGIN      
        1 tccccgcacg cacccagcaa gtctccggga aagccctcga caacgcaccc cgtatacgag
       61 tattcagctc taacaaagat agagcctctt tctcacccga taagttattt agattcggcc
      121 gcatatttac gctcttgttt ataagctttg tacacatccg ggatgccata tcccaactca
      181 gcgtccggtt gctcaaactt actgccgaaa cgctgcaaaa gatgatactt aaggagcata
      241 tctacaaata cggtgaaaag ctccccaacg aaaacgagct ttccgcaaag ctcggcataa
      301 gccgtacaac cctgcgtgag gcaatacgca tacttgcgtc cgacgggttg cttgaagtca
      361 ggcgcgggct cggtactttt gtggcacagc cgctcaatca atatgccgag ggaagcatag
      421 actttcagga gttttcaaag ctcaaaataa cgctgagaga cctgtatgag acgcgtatga
      481 ttttcgaacc cgaagcggcg gctcttgcca gccgcagagc taccgaggac gagataaagg
      541 agatactgca cctcggagac atttgccaac aagagctttt gcgcgacccg cagggcaagc
      601 gaagaataga ctctgaggcg gctttccacg gcgcaatatt aaaggcgtcg cataatgact
      661 ttttaagtcg gtttatgccg atacttaccg agaccataga gcaaaccttt gccctcaatt
      721 tcaatcttga tatgatagcc gaacaggcgt acaaagatca tatacttata atggattttc
      781 ttgaaaagcg tgacgcggta gctcttaaaa gcgcagttac catacatttg caccatgcgt
      841 tgctcacgga gcaaatatct ctggaagaaa atgaaaaaaa gtattgacaa ccgttgtttt
      901 cgcgttataa tggttgtatg acaatgactc gtagatgtca tacaactaaa atataaatat
      961 ttgttaatgt tcaggaggaa aaattatggc agaagcaaga atgttttatg agaaagactg
     1021 taacctgtcc ttactcgacg gtaagaccat cgccgtaatc ggctacggca gccagggtca
     1081 cgctcacgct cttaacgcaa aggagtcggg ctgcaatgtt atcatcggcc tttatgaggg
     1141 cagtaaatca tgggctaagg cagagcagca gggctttgag gtttataccg ctgccgaggc
     1201 tgcaaaaaaa gccgacatta ttatgatact catcaacgat gagcttcagg cagatatgta
     1261 taagaaggat atcgagccca accttaaaga gggcgatatg cttatgttcg ctcacggctt
     1321 caacattcat ttcggatgca tcaagccgcc gaagtttgtc gatgttacaa tgatagctcc
     1381 caaggctccc ggtcacaccg tcagaagcga atatcaggca ggcaagggta ctccgtgcct
     1441 tgtagctgtt nnnnnnnnnn gtcttgcaat aggcggtgca agagcaggcc ttcttgagac
     1501 cacgttccgc accgagaccg aaaccgacct cttcggtgag caggctgttc tttgcggcgg
     1561 cgtttgcgct cttatgcagg caggctttga gactctttgc gaggcaggct atgacccgag
     1621 aaacgcttac ttcgagtgca tccatgagat gaagctcata gttgacctta tttatcagag
     1681 cggttttgca ggtatgagat attcaatctc caacaccgct gagtacggcg attacatcac
     1741 cggacccaag attgttaccg aagaca
//
LOCUS       DBMDVO010000276         1765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32235_length_1765_cov_1.233173, whole genome shotgun sequence.
ACCESSION   DBMDVO010000276 DBMDVO010000000
VERSION     DBMDVO010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32235_length_1765_cov_1.233173"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="ACZPE2_04605"
     CDS             <1..1143
                     /locus_tag="ACZPE2_04605"
                     /inference="COORDINATES: protein motif:HMM:NF023591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319163.1"
                     /translation="LSFVADKENIDLTPEAAMLISRIADGALRDALSLLDRCASYGTE
                     ITEKVASDAAGIAGREHLFELVDAVISHDSPRALTIIDELYNNSCDMERLLSELLSHF
                     RNMMVALTVPNFRELVICPDSEIEIIRRQAKSLTLESVLSCMDRIGDAIVEIKRGADR
                     RTSAEMAVIRLTSPKLDTDTAAVLRRISDIEVKLKNGVFKPSSESLESDFAANASPVK
                     QSEPNEAQTQQVKNEKPESKEAVSDKTPAAYDEPNNAEPKVNETSQDKNREIKSDEVF
                     ENWAEVVDILSRTSPALTGFLNGSTAFVHPGGFLLIKSSNSILGQMLMTGNFSNDIVK
                     AVYEVSGKKYRIGIYNDEHSEAKPKKDPLDGLLSRAKELGINVIDQ"
     gene            1176..1520
                     /locus_tag="ACZPE2_04610"
     CDS             1176..1520
                     /locus_tag="ACZPE2_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1319164.1"
                     /translation="MKARIPNQGPTGANMMKKLQEVQSNMSALQDELAAAEYNASAGG
                     GAVEVTVNGSHEIKAIKIKPEVIDPEEPEMLEDLLMASLNEAMRKADETAEREMNKLT
                     GGLNIPGMGGMF"
     gene            1520..>1765
                     /locus_tag="ACZPE2_04615"
     CDS             1520..>1765
                     /locus_tag="ACZPE2_04615"
                     /inference="COORDINATES: protein motif:HMM:NF045310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HIR1319165.1"
                     /translation="MGYNIPALEKLIDEFRKMPSVGTKSAERMAFYVLGLDDNGTAEL
                     ANAIVTAHEKIHQCKICRNLTEDEICPICSNEKRDRST"
BASE COUNT          538 a          378 c          448 g          401 t
ORIGIN      
        1 ctttcgtttg tagccgataa agaaaatatt gatttaaccc ccgaggcggc aatgcttata
       61 tcacgtattg ccgacggtgc gctccgtgac gccttgtcgc tgcttgacag atgcgcgtct
      121 tacggaaccg aaataaccga aaaggtggca agcgacgccg caggcatagc cggcagagag
      181 cacctttttg agcttgttga cgccgtaatc tctcacgata gtccaagggc actcacgata
      241 atagatgagc tttacaataa ttcctgcgat atggaacgtc ttttaagtga attactctct
      301 catttccgca atatgatggt tgcactgacg gtgcctaatt tcagagagct cgttatttgc
      361 cccgattccg aaatagagat aatacgccgc caggcaaaga gtctgactct tgagagtgtt
      421 ctctcgtgta tggacagaat cggtgacgcc atagtcgaaa tcaaaagggg agccgacagg
      481 cgcacaagtg ccgaaatggc tgttatcaga ctgacaagtc cgaagcttga tacggatacg
      541 gcggctgttt tgcgcagaat ttctgatatt gaagtaaagc ttaaaaacgg cgtgtttaag
      601 ccttcttccg aatctttgga gtcggatttt gccgcaaacg catctccggt gaagcaaagc
      661 gagccgaacg aagcgcaaac acagcaggtt aaaaacgaaa agcccgaaag caaagaggct
      721 gtttccgata aaacgcctgc cgcttatgat gagccaaaca atgcggagcc gaaagtaaat
      781 gagacgtcgc aagataaaaa ccgagaaata aaatcggacg aagtgtttga aaattgggct
      841 gaggttgtcg atatattgtc gcgcacttct ccggctctta cgggattttt aaacggctcg
      901 acggcgtttg ttcatccggg cggattttta cttataaaat cgtcaaattc aatactcggc
      961 caaatgctga tgacaggcaa tttttcaaac gacattgtaa aggccgtgta tgaggtcagc
     1021 ggcaaaaaat atcgtatagg aatatacaat gacgagcatt ccgaggcaaa gccgaaaaaa
     1081 gacccgttag atgggctttt gagcagggca aaggaactcg gcatcaatgt tatagaccag
     1141 taaataagat taaattggaa ggaaatggta ataatatgaa agcaagaatt ccgaaccagg
     1201 gtcctaccgg agccaatatg atgaaaaagc tccaggaggt tcaaagtaat atgagcgcgc
     1261 ttcaggatga gcttgccgca gcggaataca atgcgtcggc aggcggcgga gcggttgagg
     1321 ttacggtaaa cggctcgcat gaaattaagg ctatcaaaat caaacccgag gttatcgacc
     1381 ccgaagagcc cgaaatgctt gaggatcttc ttatggcttc tctcaacgag gctatgcgta
     1441 aagccgacga aaccgcagag cgtgaaatga ataagcttac cggcggactt aatatccccg
     1501 gaatgggcgg tatgttttaa tgggatacaa tatccctgcg cttgaaaagc tgatagacga
     1561 atttcgcaaa atgccgtcag taggcacaaa atcggcggaa agaatggcat tttatgtgct
     1621 cgggcttgac gataacggga ccgcagagct tgcaaatgcc atagtgacgg ctcacgaaaa
     1681 aatacatcag tgcaaaattt gccgcaatct taccgaagat gaaatctgcc cgatttgcag
     1741 taatgaaaag cgcgacagat ctaca
//
LOCUS       DBMDVO010000277         1758 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32419_length_1758_cov_1.126735, whole genome shotgun sequence.
ACCESSION   DBMDVO010000277 DBMDVO010000000
VERSION     DBMDVO010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32419_length_1758_cov_1.126735"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(99..692)
                     /locus_tag="ACZPE2_04620"
     CDS             complement(99..692)
                     /locus_tag="ACZPE2_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1319166.1"
                     /translation="MSVIANLFSAAMYAFVLQNLIFTGGYGASEAVRMAAKPKQLFPL
                     TAFITFFSTFGSIFCVLLEQIPFISALSEIQHSLIFLLVICILYVLMLILLKAVFKAK
                     KRTVRRLGVAAFNTLVLAVPFINYRATFGIAEAIGVGIGSGLAFIVAVLMINFGLRRL
                     VENKNIPECFKGTPAVFIYVSILALAFSGVSGKGLSL"
     gene            complement(689..>1758)
                     /locus_tag="ACZPE2_04625"
     CDS             complement(689..>1758)
                     /locus_tag="ACZPE2_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1319167.1"
                     /translation="KMPDKKKKSVSVIRSSAIIKNPVLFEAIGIAPVVAMAVSLKSAI
                     ILSFVSLVELLLIECLACLLMKKLKGSVRKMIYAILGLLINIPLFMLFRYLAPNETAS
                     AGIFLPLLAVNSLIALHCERFAVKHNFKATALDAVSAGFSYAAVILIVGVVREILGSG
                     TVYSVKLNIPVKLPGLLMPFGGFLLLGFMAAALKAIINKKYPEKHPDASFDLSEVKQS
                     HVIKLREFLDTDLDFFELGEEEPQNEKEKPHTDKRKSSAAKRKTPSLSVKKTASAPAK
                     SKKGTGKNGASRSSTADTERQKDKSFGSSAEFEEILAELEKYKQYTAFDDSSASHSDT
                     DKSPEKDGGAETQQPKGGEDK"
BASE COUNT          438 a          431 c          416 g          473 t
ORIGIN      
        1 tacccgagcg tctgcccgga taattttttg aaaacggata atagctcttt ttacggggct
       61 taatatctct gttgtcaaac gccattttct acggtccttt acaatgataa tcctttgcct
      121 gagacgcccg aaaaagcgag cgccaaaatc gaaacatata taaatactgc cggtgtgccc
      181 ttaaagcatt ccggaatgtt tttgttttct accaagcggc gcagcccgaa atttatcata
      241 agcaccgcaa ctataaaggc aagtccggag cctataccta cgcctatcgc ctcggctatg
      301 ccgaaggtcg cgcggtaatt tataaacggt acggcaagca caagagtatt gaatgccgcc
      361 acgccgagac gccttacggt tcgctttttg gccttgaaaa cagccttcag caaaatgagc
      421 ataagaacat acaaaatgca tatcacaagc aggaaaataa gcgaatgctg tatttcgctg
      481 agagcactta taaacggtat ctgctcaagc agtacacaaa aaatactgcc gaatgtcgaa
      541 aagaatgtga taaacgccgt gagcgggaaa agctgcttcg gctttgccgc cattctgacg
      601 gcctccgagg caccgtagcc gccggtaaaa atcaggtttt gaagcacaaa tgcgtacatc
      661 gccgccgaaa aaagatttgc tatgacactc atttgtcttc accgcccttc ggttgttgtg
      721 tttccgcgcc gccgtccttt tcagggcttt tgtccgtgtc gctatgtgac gccgagctgt
      781 cgtcaaaagc agtgtactgc ttgtatttct caagctccgc gagtatctct tcgaattcgg
      841 cggatgagcc gaagcttttg tctttttgcc gctcggtgtc ggcggtactg cttctgctcg
      901 cgccgttttt gcctgtacct tttttgctct ttgccggagc ggacgcggtc tttttgacgg
      961 ataatgacgg agtttttcgc ttagccgcgg agcttttcct tttgtcggtg tgaggctttt
     1021 ccttttcgtt ttgcggttct tcctctccta actcaaaaaa gtcaaggtcg gtgtcgagaa
     1081 attcgcgcag ctttataacg tgcgactgct tgacttcgga gaggtcaaac gaggcgtcgg
     1141 gatgcttttc ggggtatttt ttgtttatta tcgcctttaa cgccgcagcc ataaagccga
     1201 gcagtaaaaa tccgccgaac ggcattaaaa gacccggcag ctttaccgga atgttcagtt
     1261 ttacgctgta aaccgtgccg ctgccgagta tttcacggac aacgccgact ataagtatta
     1321 ccgcggcata gctgaagcct gccgaaacgg catcgagagc cgtggcctta aaattgtgct
     1381 taaccgcgaa ccgttcgcag tgcagcgcga taagcgaatt taccgcaagc aacggcaaaa
     1441 atatacctgc gctcgccgtt tcgttggggg caaggtaccg gaaaagcata aacagaggta
     1501 tgtttataag caatcccaat atcgcatata tcatttttcg cacgctgccc ttcagctttt
     1561 tcataagcag gcaggcgaga cattctataa gtaaaagctc aactaaggac acaaacgaga
     1621 gaattatggc ggatttgagc gataccgcca tggcaacgac gggtgcaatg cctatggctt
     1681 caaacagcac gggatttttt attattgcgc tgcttctgat aacggaaacg ctttttttct
     1741 ttttatcagg cattttcg
//
LOCUS       DBMDVO010000278         1752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32586_length_1752_cov_1.007268, whole genome shotgun sequence.
ACCESSION   DBMDVO010000278 DBMDVO010000000
VERSION     DBMDVO010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32586_length_1752_cov_1.007268"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="ACZPE2_04630"
     CDS             complement(<1..483)
                     /locus_tag="ACZPE2_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR1319168.1"
                     /translation="MNKKQCRKYIVPVILTALVCLFLFTRPEICSEGVRSGILLAAET
                     VVPSLFPFMTVASFLLRSGLGEAAGKPFDKVCEKLFRLPGVLAPVIIMSQIGGFPIGA
                     KMTYELLKKNAITENEAQRACLFCINAGPAFVIGTVGSEMLGSVKAGVLLYISTVSAS
                     F"
     gene            568..858
                     /locus_tag="ACZPE2_04635"
     CDS             568..858
                     /locus_tag="ACZPE2_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1319169.1"
                     /translation="MTARRKQRKKASEQKIVGALDMPKILSPSVPHIETEGNKEAVVD
                     GCFGITEYDNDRISLNAGSLTITFCGSDLTLCSYSDEQTVIKGSIISIEFTG"
     gene            868..>1752
                     /locus_tag="ACZPE2_04640"
     CDS             868..>1752
                     /locus_tag="ACZPE2_04640"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1319170.1"
                     /translation="MSIIRFLRFIFGYVEFCAEGGFPERFINLCSVYHIRLWDVKRVK
                     DKLYAKTMLRDYKNIREAVRRSGVKTRIIKKTGLPFFTFKQRKRVGLVIGVAAAIITV
                     SYLSTMIWTVSVSGNENISDEQILNVFSSLGVKPGAKRRDINAKEISDEALKIFDGDL
                     SFAVVNLNGSNASVEVRESVPAPKIEDNETPCNIVASEDGVVTKVQLYSGQEEIKAGS
                     AVLKGNLLISGVKTNQDKTESLVHAAGNVYAEVEKNISSDFENSEFCAPSREKIRYAL
                     YFFNVKIPLGGVPDYGEKY"
BASE COUNT          509 a          360 c          425 g          458 t
ORIGIN      
        1 aaatgatgcc gaaacagtag atatgtataa cagcacgccg gctttgacgc ttccgagcat
       61 ttccgagcca accgtcccta tcacaaaggc ggggcccgcg tttatgcaaa aaagacaagc
      121 tctttgcgcc tcgttttcgg ttatagcatt tttcttgagc aattcatatg tcatttttgc
      181 gcctatggga aatccgccta tctgtgacat aataataacc ggagccaaaa cgccaggaag
      241 cctgaacagc ttttcgcaca ccttatcaaa cggttttccc gcggcttctc cgagacccga
      301 gcgcagaaga aaggaggcca ccgtcataaa cggaaacagg gacggcacta ccgtttccgc
      361 cgccaaaagt attccgcttc tgacgccctc ggagcatatc tccggccttg taaacaaaaa
      421 caggcacacc aaagcagtca atataaccgg aactatatat tttcggcatt gctttttatt
      481 cattcttttc acccttttcc gtaatttaaa tatatgaggc ggcatattta tatatgagct
      541 taaaatgact tttcggaggg atcggttttg accgcaagaa gaaagcagcg aaaaaaagcg
      601 tccgagcaaa aaattgtcgg cgcgcttgat atgcctaaaa ttttgagccc gtccgtgccg
      661 catattgaga cggagggcaa taaggaggcg gtcgttgacg gctgcttcgg aataacggaa
      721 tatgacaatg acaggatttc gcttaatgca ggctctttga ccattacctt ttgcggcagc
      781 gaccttacgc tgtgttctta ttccgacgaa cagacggtga taaagggcag tataatttcg
      841 attgaattta cgggctgagg tgattttatg tcgataatac gttttttgag atttattttc
      901 ggatatgttg aattttgcgc ggagggtggt tttcccgaac gctttataaa tctgtgctcc
      961 gtttatcaca taagattgtg ggatgtaaaa cgagtaaaag acaagctgta tgcaaaaact
     1021 atgcttcgag actataaaaa tatcagggag gcggtaaggc gttccggggt aaaaacaaga
     1081 ataatcaaaa aaacgggttt gccttttttt acatttaagc agcgaaagag agtgggactt
     1141 gtaataggcg ttgccgcggc gataatcacc gtttcgtatt tatcgactat gatatggact
     1201 gtatccgtca gcggaaacga aaacatatca gacgagcaaa ttctcaatgt attttcttca
     1261 ttgggagtaa aacccggagc aaagcgccga gatataaacg caaaagagat ttcagacgag
     1321 gctttaaaaa tatttgacgg tgatttgtcg tttgcggttg tcaatctgaa cggctctaat
     1381 gcgtccgtgg aggtgcgtga aagcgtgccg gcaccgaaga tagaggacaa cgaaacccca
     1441 tgtaatatag ttgcatcgga ggacggcgtg gtgacaaagg tgcagttgta ttcgggtcaa
     1501 gaggagataa aggcaggctc tgccgtgctg aagggcaatc ttctcatatc cggggtaaag
     1561 acaaatcaag acaagaccga atctcttgta catgcggcag gcaacgtgta tgcggaagtc
     1621 gaaaaaaaca tttcttccga ttttgaaaat tcggaatttt gcgcgccttc gcgtgaaaaa
     1681 ataaggtatg cgctgtattt tttcaatgtt aaaattccgc tcggaggtgt tcccgattac
     1741 ggagaaaaat at
//
LOCUS       DBMDVO010000279         1751 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32616_length_1751_cov_0.967273, whole genome shotgun sequence.
ACCESSION   DBMDVO010000279 DBMDVO010000000
VERSION     DBMDVO010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32616_length_1751_cov_0.967273"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="ACZPE2_04645"
     CDS             complement(<1..128)
                     /locus_tag="ACZPE2_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016988.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR1319171.1"
                     /translation="MRNADNRIPSNFHGLLVDQKSAYMFTSPPLFDVGNKSANEKI"
     gene            complement(282..1670)
                     /gene="terL"
                     /locus_tag="ACZPE2_04650"
     CDS             complement(282..1670)
                     /gene="terL"
                     /locus_tag="ACZPE2_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HIR1319172.1"
                     /translation="MDKNLIMLEAKKELARREFFYFCHLTAPLFYKSEREFLVRLCNE
                     MQSFYESDENALIINLPPRHGKSRTASMFVEWVLGRNQSEKIMTGSYNETLSTTFSKA
                     VRNAIQEEKADADKIIYSDIFPNVKIKQGDGAMNLWSLEGGYNNYLATSPSGTATGFG
                     ASLLIIDDLIKNAEEAYNETVKEKHWEWFTNTMLSRLEEKGKIIIIMTRWASGDLAGR
                     AIEYFSENNISHRVITMKAVCDDGNMLCDEILSRSSYDLKIKAMGADIASANYQQEPI
                     DLQGKLYTTLKTYDSLPPITQIQSYCDTADTGADYLCNIIYGIYGKEVYVIDVYYTDE
                     PMEITEGETARRLYENNVNLAKIESNNGGRSFARRVREILAEKYGSNFTTVKWFHQSN
                     NKEARILSNSTWVMEHIYFPCDWHIRFPEYYKAMTTYQREGRNKHDDAPDATTGIAEM
                     MNRKKGGLSILK"
BASE COUNT          399 a          410 c          335 g          607 t
ORIGIN      
        1 cttattttct cattcgccga tttattcccg acatcaaata acggcgggga cgtaaacata
       61 tatgcggact tttggtctac aagcaagccg tgaaaatttg acggtattct gttatcggca
      121 tttctcaaag gcttttcgtc ctcactatgc tatatgtgca aaatatcgtt gtcgtttaag
      181 taataccttt ccgccgtctg cactctcgat ataaaattct cgtgtccggg tatatatttt
      241 tttatcagct ttttcactgt ttccaaatcc atttttatca cctactttaa aattgacagt
      301 ccgccctttt tcctgttcat catttccgca atacccgttg ttgcgtcggg tgcgtcgtcg
      361 tgtttgttcc tgccctcacg ctgatatgtt gtcatcgcct tatagtattc gggaaaacgt
      421 atatgccagt cgcaaggaaa atatatatgc tccattaccc aagtgctgtt ggataatatt
      481 cgtgcctctt tgttattgct ttggtgaaac catttcaccg ttgtaaaatt actgccgtac
      541 ttttcggcaa ggatttcacg cacacgtctt gcgaacgaac gtccgccgtt attgctttca
      601 atctttgcaa ggttgacgtt gttctcgtat aatctgcgtg ccgtttcacc ctctgtaatc
      661 tccataggct cgtcggtata atacacgtct atgacgtata cttctttgcc gtatatgccg
      721 tatattatgt tgcagagata gtccgcacct gtatcggcgg tatcgcaata tgattgtatt
      781 tgcgtaatcg gcggtaaact gtcgtatgtt ttaagcgttg tgtagagttt gccttgcaaa
      841 tcaatcggct cttgctgata gttcgcactt gctatatccg cacccatcgc cttaatcttt
      901 aagtcgtaac tgctccgtga caagatttca tcacatagca tattgccgtc atcacatacg
      961 gctttcatcg tgattactct gtgcgatatg ttgttctcgc tgaaatattc aatcgcacgt
     1021 cccgcaaggt cgccagacgc ccatcgtgtc attataataa ttatcttgcc tttttcttca
     1081 agtcgtgaaa gcattgtgtt cgtaaaccat tcccaatgct tttctttgac tgtttcgttg
     1141 tatgcctcct cggcattttt gataaggtcg tcgattataa gtaaactcgc tccgaaacct
     1201 gtcgcagtac cggacggcga tgtggcaaga tagttgttgt aaccgccctc aaggctccac
     1261 aggttcattg ctccgtcacc ttgctttatt ttcacattcg gaaatatgtc actgtaaata
     1321 atcttatccg catcggcttt ttcttcctgt atcgcattac gcaccgcttt tgaaaacgtt
     1381 gttgacagtg tttcattgta tgaaccggtc attatctttt cactttgatt tctgccgagt
     1441 acccattcga caaacatcga cgcagtacgg ctcttgccgt gacgtggcgg taaattgata
     1501 atcagtgcgt tttcgtcact ttcgtaaaac gattgcattt cattgcataa ccgtacaaga
     1561 aattctcgtt ccgatttgta gaataacggt gcggttaaat ggcaaaaata aaagaactcg
     1621 cgtcgtgcaa gttctttctt cgcctcaagc attattaagt ttttatccat cacctatcaa
     1681 cttccttaat tcgtcgatcg taagatttgc cataggattg tttatgtcca ttgtgccact
     1741 gtgcgttatt t
//
LOCUS       DBMDVO010000280         1750 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32644_length_1750_cov_0.821104, whole genome shotgun sequence.
ACCESSION   DBMDVO010000280 DBMDVO010000000
VERSION     DBMDVO010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32644_length_1750_cov_0.821104"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            355..1395
                     /locus_tag="ACZPE2_04655"
     CDS             355..1395
                     /locus_tag="ACZPE2_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1319173.1"
                     /translation="MIDKKYADYSWEKASALVNIDSPTGFTKKAAEFVKKEFSALGFP
                     AEYTVKGGVLADLGGKDDNNALLLEAHTDTLGGMVAEVKSNGRLRITPLGGMNACNAE
                     TENVRIYTRSGKIYEGALQICNASIHVNGDFSTVSRTWDSVEIVIDEDVKTDEDTRKL
                     GIETGDIVCFEPRIRRTESGYLKSRFLDDKLSVGILLGFAKYLKDNGIVLPRRVYVHV
                     TVYEEVGHGGSSTVPGGVTEAISVDMGCVGDGLKCTEKQVSVCAKDSGGPYSYDTVGK
                     LIEAAKAMGADYAVDVYPHYGSDVEATLRSGADIQHGLIGAGVYASHGYERSHIDGVY
                     NTLKLLCGYLNV"
     gene            complement(1434..>1750)
                     /locus_tag="ACZPE2_04660"
     CDS             complement(1434..>1750)
                     /locus_tag="ACZPE2_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR1319174.1"
                     /translation="DRVEEIAKENKVKKVTKLTLEIGEVSSIVPSYFSDCFEWAKKKT
                     DYMKDTELEMIILEGLSYCRDCKKTYKTTEYAKKCPHCGSDNTYLVTGDEMRIKDIEV
                     IG"
BASE COUNT          451 a          370 c          419 g          510 t
ORIGIN      
        1 gaaaggctgc cttttttata aatcccaaag cggtttaaaa ataaaatgta atgtaattcg
       61 cttttgactt tgcttttgac tttgcttttg acttctgctt ttacttttac ttttgtctat
      121 gtctgagcct gtgcctgtgt ctgtcttgta cctttgccta tgcccgggtc tgtgcggctt
      181 tgcaggcagt ttatttgtta cgtatcgaca cactttagat atatattaac tacacttgtg
      241 ctgataagta acatttttca ctttactaca ctgaaaatca acgcatattt gttttttcgg
      301 cgcggttatg gtaaactaat agtataattt ttcggaaagc tgcggtgata agctgtgata
      361 gataaaaaat atgcagatta ctcatgggaa aaggcgagcg cgctggtaaa tattgattct
      421 ccgacgggtt ttaccaaaaa agccgccgaa ttcgttaaaa aggaattttc ggctctcgga
      481 tttcccgccg aatacacggt aaagggcggc gttcttgccg acctcggcgg aaaagacgat
      541 aacaatgctt tgcttttgga ggcgcacaca gataccctcg gcggaatggt cgccgaagtc
      601 aagagcaacg gcagactgag aataacgccc ctcggcggta tgaatgcctg caacgccgaa
      661 acggagaatg tcagaatata tacccgttcg ggcaaaatat atgagggcgc gcttcagatt
      721 tgtaacgctt caattcacgt taacggcgat ttttcgactg tttcgcgtac ttgggacagc
      781 gtcgaaatcg tcatcgacga ggacgtaaaa accgatgagg atacaagaaa actcggcata
      841 gaaacgggcg atatagtttg ctttgaaccg cgtatacgcc gtaccgaatc cggatattta
      901 aaaagcagat ttcttgacga taagctctct gtgggcatat tgctcggttt tgcaaaatat
      961 cttaaagaca acggtatagt tttaccgcgg cgtgtgtatg tgcacgttac cgtttatgaa
     1021 gaggtcggcc acggcggttc ctcgactgtg ccgggcggag ttaccgaggc aatatctgtc
     1081 gatatggggt gcgtcggcga cggattgaaa tgtacggaaa agcaggtgtc cgtctgcgct
     1141 aaggattccg gcggacctta cagctatgat accgtaggca aactgattga agccgcaaag
     1201 gcaatgggtg ccgattatgc cgttgatgta tatccgcatt acggctcgga tgttgaagcc
     1261 acattgcgtt cgggcgcaga tatacaacac gggctgatag gagccggagt ttatgcgagc
     1321 cacggctatg aacgcagcca tatagacggc gtttataata cgctgaagct gctgtgcggt
     1381 tatttgaatg tataaatata aacggagctg taaaaaaata cagctccgtt tttctacccg
     1441 ataacttcaa tatcctttat ccgcatctcg tcgccggtga caagatatgt attgtcactg
     1501 ccgcagtgcg gacatttttt tgcgtattcg gtggttttat atgttttttt acagtcgcga
     1561 cagtaggaga gaccctcaag gattatcatt tcaagctcgg tgtctttcat ataatccgtt
     1621 tttttctttg cccactcaaa gcagtccgaa aaatatgacg gaacaatcga cgaaacctcg
     1681 cctatttcaa gagtcagctt ggtgaccttt tttactttgt tttccttggc aatctcttct
     1741 actctgtcta
//
LOCUS       DBMDVO010000281         1749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32655_length_1749_cov_1.275485, whole genome shotgun sequence.
ACCESSION   DBMDVO010000281 DBMDVO010000000
VERSION     DBMDVO010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32655_length_1749_cov_1.275485"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="ACZPE2_04665"
     CDS             complement(<1..987)
                     /locus_tag="ACZPE2_04665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1319175.1"
                     /translation="MDDFNNNTPSGNEQQNAPESTGNFQSAPEAGNDAQRANSGSPYN
                     GGYQPNNGYPYSNGGGNTNNGSSNANGSSNGYSSGNNGYYGSNGAYRPSSTYSGNNSY
                     VYYGNSGQPPKKPVDNKPSKHKKGGVIALIIAVVVCVALGVTGIAVSLSNKDGAKKQT
                     SEKASADSSTVTINETPTASSTDKSGSLTAAGVYDKIKDSSVGILVYSDSRSSSASGQ
                     GSGVIVGEDSSKQYTYIMTCAHVISGGGSVRVQLSDETQYDATVVGYDSKTDIGVLKI
                     KATGLKAAEFGDSDKLSVGETVYAIGNPGGTAFAGSFTNGIVSAISRPVNSQI"
     gene            complement(1104..>1487)
                     /locus_tag="ACZPE2_04670"
     CDS             complement(1104..>1487)
                     /locus_tag="ACZPE2_04670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1319176.1"
                     /translation="LDRLHSVTVTADEDMIHQVIYNLVDNAVKFTENGTISVFADDDE
                     NATLIKVVNTGAGISSEEREKIFERFYKVDKSRSYDVKGAGLGLYICKTIVEMHGGSI
                     GCNSEEGKYTEFWFTIPKYLPPKQL"
     assembly_gap    1488..1497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1498..>1749)
                     /locus_tag="ACZPE2_04675"
     CDS             complement(<1498..>1749)
                     /locus_tag="ACZPE2_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1319177.1"
                     /translation="GFIDGILDGTIPESQQKHYLSIVSDETKRLSRLVTAMLNMSKIE
                     AGELKLNPKAFDISAEIFNVLLSFEQIIERKHIEISGLD"
BASE COUNT          364 a          428 c          424 g          523 t
ORIGIN      
        1 tatctggctg tttacggggc gagaaatcgc tgatactata ccgtttgtga acgagcctgc
       61 aaatgccgtt ccgccggggt tgcctatcgc gtaaaccgtc tcgcctacgg agagcttgtc
      121 gctgtcgccg aattccgcgg ctttaagacc ggtagccttg atttttaaaa ctccgatgtc
      181 ggttttagag tcatatccca ctacggtagc gtcgtactgt gtttcgtcgg atagctgaac
      241 tctgacggag ccgccgcccg aaattacgtg agcgcaggtc ataatatatg tgtactgctt
      301 actgctgtcc tcgccgacta ttacgcccga gccctgaccc gatgcggagg aggaacggct
      361 gtcggagtat acgagtatac cgacggagct gtcctttatc ttatcataaa cgcctgccgc
      421 ggtaagactg ccgctcttat cggtagagga agcggtggga gtttcgttta tcgtaacggt
      481 tgagctgtct gcggaagcct tttcgcttgt ctgctttttg gcaccgtcct tattgctgag
      541 agaaaccgca atgcccgtaa caccgagtgc cacacaaacc acaactgcga ttatgagggc
      601 tataacgccg ccttttttat gctttgacgg tttattgtca accggctttt tcggcggttg
      661 gcctgaattt ccgtaataga catagctgtt atttccgctg tatgtcgagc tcggacggta
      721 tgcgccgttt gagccgtagt agccgttgtt tccgcttgaa tagccgttac tgctgccgtt
      781 tgcatttgaa ctgccgttat ttgtgtttcc gccgccgttc gagtaagggt agccgttatt
      841 cggctgatag ccgccgttat aaggactgcc gctgttcgct ctttgagcgt cattgccggc
      901 ttcgggagca ctttggaaat tacccgtgct ttcaggggcg ttttgctgct cgtttcccga
      961 aggggtgttg ttattgaaat cgtccataaa aagatctcct tgatttatct gatgaagaca
     1021 agagcttttc tcttgtcgtt gtcataatca taaccgacgg ttattacaga actattaact
     1081 cgatttaaat aatctgaata cttttacagc tgcttaggcg gaagatattt cggaatagta
     1141 aaccaaaatt cggtgtattt gccctcttca ctgttgcagc ctatcgaacc gccgtgcatt
     1201 tcgactatcg ttttgcatat ataaagaccg agtcccgcgc ccttgacgtc atagctgcgc
     1261 gatttatcca ccttgtagaa gcgctcgaat attttttcgc gctcctccga ggaaataccc
     1321 gcgcccgtgt ttacgacctt tataagtgtt gcgttttcgt catcgtctgc aaatacgctt
     1381 atggtgccgt tttccgtgaa tttgaccgcg ttgtccacaa gattgtatat gacctgatgt
     1441 atcatatcct cgtcggcggt gaccgttacg gaatgaagcc tgtcaagnnn nnnnnnntgt
     1501 caagcccgga tatttcaatg tgcttgcgct ctattatctg ctcgaacgac agcagcacgt
     1561 tgaatatttc cgcacttatg tcaaacgcct tcgggttcag ctttaattca cccgcttcga
     1621 ttttggacat attcagcatt gccgtaacaa gccttgacag acgcttggtc tcatcggaaa
     1681 caatgctcaa atagtgtttt tgctggcttt cgggaatggt gccgtcaagg attccgtcta
     1741 tgaagcctc
//
LOCUS       DBMDVO010000282         1746 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32723_length_1746_cov_1.399392, whole genome shotgun sequence.
ACCESSION   DBMDVO010000282 DBMDVO010000000
VERSION     DBMDVO010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32723_length_1746_cov_1.399392"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="ACZPE2_04680"
     CDS             complement(<1..1490)
                     /locus_tag="ACZPE2_04680"
                     /inference="COORDINATES: protein motif:HMM:NF044849.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319178.1"
                     /translation="MIRINELKLPLDEDEKELYRLAAKALKIGERHIRELTICKKSVD
                     CRKKDDIKFIFSVDVALDIDEKRVASKAGNRVSLSEKSVYTLPPVKRKSTLRPVIVGF
                     GPAGMFAALTLTEAGLSPIIIERGETVDERTASVKSFWRTRVLNPESNVQFGEGGAGT
                     FSDGKLTTGIKDKRCRRVFEKFCEHGAPSEIMYSATPHIGTDRLAGVVKAIRKTVEER
                     GGEFMFNTKLCDLIIYNGHIQGITVCHKDGSKEDIETDTVILSIGHSARDTLEMLRLK
                     GIDMMQKPFSVGVRIEHRQEMINKTQYGKFASHPKLNAANYKLACHPQGGRGAYTFCM
                     CPGGTVVCASSEEGGVVVNGMSEYARDGENADSALLVGIEPELFPSSDVLSGMYMQRE
                     IERHAFKMGGSDYTAPAQKVGDFMNNLASSSLGSVKPTCPTGVRLTNLRSLLPAKVTE
                     TILEALPKMDKMLPGFASNDALFTAPETRSSSPVRIIRDELFQSNVR"
     gene            complement(1491..>1746)
                     /locus_tag="ACZPE2_04685"
     CDS             complement(1491..>1746)
                     /locus_tag="ACZPE2_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319179.1"
                     /translation="LQIASAIKSLKFSVTGVRGFDFAQVTHGGAALSEFDNNTLEAKK
                     VKHLYAVGEALNVDGPCGGYNLGWAWSSAFLAAESVLKEL"
BASE COUNT          420 a          478 c          386 g          462 t
ORIGIN      
        1 cgcacattcg actggaaaag ctcgtctcgg atgatgcgca cgggagatga gcttcgcgtt
       61 tcgggtgctg tgaaaagagc atcatttgaa gcaaagcccg gaagcatttt gtccattttc
      121 ggcagtgctt cgagtattgt ttccgtaacc ttagcaggga gcaacgagcg cagatttgta
      181 agccttaccc ccgtaggaca cgtcggcttt acgctgccga gcgaagaaga tgcgagattg
      241 ttcataaaat ctccgacctt ctgtgcagga gcggtgtaat cgctgccgcc cattttaaat
      301 gcgtgacgct ctatttcgcg ctgcatatac attcccgaaa gcacgtccga gctcggaaaa
      361 agctccggct ctataccgac aagcaatgct gaatcggcat tttcgccgtc tcgcgcatat
      421 tcactcattc cgtttacaac aactccgccc tcttcgcttg acgcgcaaac cacggttccg
      481 ccgggacaca tacaaaacgt atatgcgccc ctgccgccct gcgggtgaca ggcaagcttg
      541 taatttgcgg cattgagctt tgggtgcgaa gcaaatttac cgtactgggt tttatttatc
      601 atttcctgcc tatgctctat tctcactccg accgaaaacg gtttttgcat catatcaatg
      661 ccttttaagc gaagcatttc gagcgtgtca cgagccgaat gccctatcga gaggataacg
      721 gtatcggttt caatgtcctc tttgcttcca tccttgtgac aaaccgtaat gccctgaatg
      781 tgaccgttat aaattataag gtcacagagc tttgtattga acataaactc tccgccgcgt
      841 tcctctaccg ttttgcggat agcttttaca actcccgcca aacgatccgt gcctatgtgc
      901 ggagttgcgg aatacataat ctcgctcggt gcgccgtgct cgcaaaattt ttcaaacaca
      961 cggcggcaac gcttgtcctt tattcccgtg gtaagcttac cgtccgaaaa agtgcctgca
     1021 ccgccctcgc cgaactgcac attcgactcg ggatttaaaa ctctcgtccg ccaaaagctc
     1081 ttgacggaag cggtgcgctc atccaccgtc tcgccgcgct ctatgataat cggcgaaagc
     1141 cccgcctcgg ttaaggtcag agccgcaaac ataccggcag gaccgaaacc gactataacc
     1201 ggtctcaagg tactcttccg ttttacgggc ggcaaggtat aaacgctttt ctcgcttaac
     1261 gaaaccctgt tgccggcttt tgaagccacc ctcttttcgt caatatcaag agccacatcg
     1321 acgctgaaaa tgaatttaat atcgtctttt tttcggcaat ccaccgattt tttacagatt
     1381 gtcagctctc gtatatgcct ttcaccgatt ttaagagctt ttgcggcaag cctgtaaagc
     1441 tccttttcgt cctcatcgag aggcaattta agttcattta ttcttatcat ttataactcc
     1501 ttcaaaacag attccgctgc aaggaacgcc gatgaccagg cccaaccgag gttgtatcct
     1561 ccgcacggac cgtcaacatt gagcgcctcc ccgacggcat acagatgttt tactttttta
     1621 gcttcgagag tattattatc aaattccgaa agagccgctc cgccgtgagt cacttgggca
     1681 aaatcgaagc cgcgaacacc cgtgacggaa aatttaagcg attttatcgc ggaagcaatt
     1741 tgtaaa
//
LOCUS       DBMDVO010000283         1746 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32729_length_1746_cov_1.085106, whole genome shotgun sequence.
ACCESSION   DBMDVO010000283 DBMDVO010000000
VERSION     DBMDVO010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32729_length_1746_cov_1.085106"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="ACZPE2_04690"
     CDS             complement(<1..1180)
                     /locus_tag="ACZPE2_04690"
                     /inference="COORDINATES: protein motif:HMM:NF025843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1319180.1"
                     /translation="MSETHEKEIIEGTVEDIRFRNEQNGYTVLSVGCDDDLITAVGVF
                     SDISVGETVKLQGEWTYHTTFGRQFKVEAFERNMPATTEQLYNYLAAGAIKGIGRATA
                     ERIVDMFGEKAFDYLESNPEMLSKVKGISPEKAEKICASYRSQFAIRSITMTLESYGI
                     GPRECLAAYKAFGGDVVGKVTENPYVLCLPEVGVPFDRVETIADNLPHRPNDSYRIKA
                     GVEHILRHNLYVDGHTCIPRDKLLRVAAEFLNTNADTVDIMTDELCRENRLVSKVFTD
                     KEYVFLIQAFRDEKSISDRIKVLLNFPPAGHSALDSEIEKIEKADNISYAEKQKLAIK
                     TAVNKGILILTGGPGTGKTTTLKGILRLFQSEGLDISLAAPTGRAAKRMTELTGKEAK
                     T"
     gene            complement(1177..1509)
                     /locus_tag="ACZPE2_04695"
     CDS             complement(1177..1509)
                     /locus_tag="ACZPE2_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319181.1"
                     /translation="MLEGFFGAVFVGLIILGVFAVVYAVLLKLLLKAKYEYYVLIPSK
                     HCGKNVSAAVYAARMKMNLIGDEDYGKVIVLDTGMTEKEKLGCQNICRETNGIYLFTA
                     EDLEEYLK"
     gene            complement(1548..>1746)
                     /gene="rnpB"
                     /locus_tag="ACZPE2_04700"
     ncRNA           complement(1548..>1746)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPE2_04700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          404 a          457 c          373 g          512 t
ORIGIN      
        1 tggtcttagc ctccttgccg gtaagctcgg tcattctttt tgccgctctt ccggtgggtg
       61 ccgcaaggct tatgtccagt ccctcgcttt ggaagagacg aagtatgccc ttgagcgtgg
      121 tggttttacc tgttccggga ccgccggtca aaatcagtat gcccttgttc accgctgtct
      181 ttatggcaag cttttgcttt tcggcatacg atatattatc ggctttttcg attttctcta
      241 tttcgctgtc aagtgccgaa tggcctgccg gcggaaaatt caaaagcacc tttatgcggt
      301 cgcttatact cttttcatcg cgaaatgcct gaattaaaaa gacgtactcc ttgtcggtaa
      361 acacctttga gacaagtcgg ttttcacgac aaagctcgtc cgtcattata tctacggtgt
      421 ctgcatttgt attaagaaat tcggcagcca cgcgcaaaag cttatcacgc ggtatgcagg
      481 tgtgtccgtc aacatataaa ttatgtctta aaatatgctc tacacccgcc tttatcctgt
      541 atgaatcatt tggtctgtgc ggaagattgt cagctattgt ctcaactcgg tcaaacggta
      601 cgccgacctc tggcaagcag agaacgtacg ggttttccgt gaccttgccc accacatcac
      661 cgccgaacgc tttatatgcg gcaaggcact cgcgcggacc tataccgtac gattcgagcg
      721 tcatggtaat gctgcgtatc gcaaactggc tgcgatatga cgcgcagatt ttttctgcct
      781 tttccggcga aatgcccttg acctttgaga gcatctcggg attgctttcg agataatcga
      841 atgctttttc gccgaacata tccacaatgc gctctgccgt ggccctgcct atacctttta
      901 ttgcgcctgc ggcaagataa ttataaagct gttcggttgt tgccggcata ttgcgttcaa
      961 acgcctcaac cttaaattgc ctgccgaagg ttgtgtgata agtccattca ccctgaagct
     1021 ttacggtttc gccgacggaa atatcggaaa atacgccgac ggccgtgata aggtcatcat
     1081 cacagccgac ggaaagcacg gtatatccgt tttgctcatt tctgaacctt atgtcctcta
     1141 ccgtaccctc tattatctct ttttcgtgag tttcgctcat ttcaaatatt cctctaaatc
     1201 ctcggcggta aaaagatata tgccgtttgt ttcacggcaa atattttggc agccgagctt
     1261 ttctttttcg gtcattcccg tgtcaagcac tataacctta ccgtaatcct catcgcctat
     1321 aagattcatt ttcattctcg cggcgtatac tgccgcagac acatttttcc cgcaatgctt
     1381 agacgggata agtacatagt attcgtattt tgctttcagc agcagtttta acagcacagc
     1441 atatactacg gcaaagacgc ccaatattat aagtcccaca aaaaccgcgc cgaaaaagcc
     1501 ctctaacatt aaaaccaccc catatcatca tatgaggcgg aaataaaaga gtgagcagcc
     1561 ttgtaagccg agttctgtcg tttaaagcag ttatctgtct aggcgtaccg ttgccggcac
     1621 gctcaagcca tccttcgaag tatttgccca gctgacaact tctgtcgatg ttgcatcggg
     1681 tagggtttgc ctggcaatgc cgttaccgac attccggtga gctcttacct cgccattcca
     1741 ccctta
//
LOCUS       DBMDVO010000284         1743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32815_length_1743_cov_0.845311, whole genome shotgun sequence.
ACCESSION   DBMDVO010000284 DBMDVO010000000
VERSION     DBMDVO010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32815_length_1743_cov_0.845311"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="ACZPE2_04705"
     CDS             <1..238
                     /locus_tag="ACZPE2_04705"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIR1319182.1"
                     /translation="VMINGLGATSLEELYIVYRAVQKKLSNIGVSIVMPHVGEYSTSM
                     EMAGMSISIFKLDEELEGLLADEAITPFYTNVNK"
     gene            254..874
                     /locus_tag="ACZPE2_04710"
     CDS             254..874
                     /locus_tag="ACZPE2_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HIR1319183.1"
                     /translation="MIDAKVLTIGFDAISKKMSENRMHLISIDQQIGDGDLGISMDNG
                     FKAAADVCCANADTADIGKLLFLAGKSFNEAAPSSLGTIVSMLLIDAAKQLKGTEVIA
                     LADFGNIIHKSMSSIMEKLDSKLGEKTFFDAVVPAAKALCEEGVVSDKQRLKNAAEAA
                     RNGVESTRDMVAKHGRAAYFGEKTLGSIDGGAEAGRIIFEAIAEQF"
     gene            899..>1743
                     /locus_tag="ACZPE2_04715"
     CDS             899..>1743
                     /locus_tag="ACZPE2_04715"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1319184.1"
                     /translation="MIDSSVDYKNLPRPTWAEIDIDAILNNINEYKKALGNKVEIIVA
                     AKGNGYGHGMLPIVKAINELDIYGFATGNMYEAIEMRKHGIDKPIQLFAHNFPETADL
                     LVKYDLMPSFVKPGDAKSFADALGKDVALKVWVKCDTGLGRLGLLPEEVLPELEYIRN
                     ETSFKIEGLYSHIGPTDSDKNPKKDEYNEGQISCFNKIIADIEAAGFEIPRYQLASTY
                     ATQRYEKAWYNTVCIGTGVFANAKPQKDSCGLKLKDCLKGIHSRLISKKTLKAGSRCL
                     NMLME"
BASE COUNT          543 a          314 c          454 g          432 t
ORIGIN      
        1 cgttatgata aacggtttag gcgcaacatc actggaggaa ctatatatag tttatagggc
       61 agtgcaaaag aagctcagta atattggtgt cagcatagta atgccgcatg tgggagaata
      121 ttcgacatcc atggagatgg ccggcatgtc tataagcatt tttaagttgg atgaagagct
      181 tgaaggtctg ttggcggatg aagccattac gccattctat acgaatgtaa ataagtaaag
      241 gagggtgttc caaatgattg atgcaaaggt gctgactatt ggatttgatg cgataagtaa
      301 aaaaatgagt gaaaacagga tgcatctgat ttcaattgat cagcaaatcg gagatggaga
      361 tctcggtatc tcaatggata acggattcaa agccgctgcc gatgtgtgct gcgctaatgc
      421 cgataccgca gacataggca aactgttatt tcttgcagga aagagcttca atgaagcggc
      481 accgtcatct ttgggtacga tcgtatcaat gttgcttata gatgccgcaa aacagctaaa
      541 aggaacagaa gtaattgcac ttgcagattt cgggaatata atacataagt ccatgagctc
      601 tattatggaa aaactggatt cgaagctagg agagaagacg ttttttgacg ctgttgttcc
      661 tgctgcgaag gcactctgtg aggagggcgt ggtatcagat aagcagaggc taaaaaatgc
      721 ggcagaggct gcccgtaatg gagtagaaag caccagagat atggtggcaa aacatggacg
      781 tgctgcctac tttggagaga agactcttgg cagcattgac ggcggcgcgg aagccggacg
      841 tataatattt gaagcaatag cagagcagtt ctaatttgct aataaaggag ttatcgtttt
      901 gattgattca tcggttgatt ataaaaatct gcccagaccg acctgggcgg aaatagatat
      961 tgatgcgatt cttaataata ttaacgaata caaaaaagca ttgggtaaca aggttgagat
     1021 aatagtcgcg gcgaagggta acggctacgg tcatggtatg cttccgattg ttaaggccat
     1081 aaatgagctg gatatatacg gatttgcaac cggcaatatg tatgaggcaa ttgaaatgcg
     1141 aaagcatgga atagacaagc ctatacagct tttcgctcat aatttccctg agaccgcgga
     1201 tctgctcgta aaatatgatt tgatgccgtc atttgtaaaa cccggagatg caaaatcctt
     1261 cgcggatgcg ctcggaaagg atgttgccct taaggtttgg gtgaagtgtg atacgggcct
     1321 cggacgtttg ggcttgctgc ctgaagaagt gcttcccgag ctggagtaca tcagaaatga
     1381 aacttcgttc aagattgaag gcctgtacag tcatatcggc ccaacagatt ctgacaagaa
     1441 tccgaaaaag gatgagtaca atgagggtca gatctcgtgc ttcaacaaga taatagctga
     1501 catagaagcg gccggattcg aaattcccag ataccagctt gcaagtacat atgctacgca
     1561 aaggtatgag aaagcatggt ataacaccgt ttgtatcgga accggagtgt ttgcaaacgc
     1621 caagcctcaa aaggattcat gcggacttaa acttaaggat tgcctgaagg gaatacattc
     1681 gcgtcttata agcaagaaaa ctctcaaggc cggcagcaga tgcctgaata tgctcatgga
     1741 aaa
//
LOCUS       DBMDVO010000285         1741 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32857_length_1741_cov_1.228659, whole genome shotgun sequence.
ACCESSION   DBMDVO010000285 DBMDVO010000000
VERSION     DBMDVO010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32857_length_1741_cov_1.228659"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="ACZPE2_04720"
     CDS             complement(<1..550)
                     /locus_tag="ACZPE2_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319185.1"
                     /translation="MKKNITITIDEELIKVAERYEAETAKSVSHFFEHAGYLMLARYS
                     VASGGGLSFEKGKEAMQLDAITVENRVLYGQAETAAQDKEPMSNKKIVKAFSEYLYKY
                     LEGVADEYHWSYEVDPESHVSWMSPELFSGETNDDSNRFLECCGEALNRGIDTDDSKL
                     VFEAVRLCMDWAGAFYDFRHGPQ"
     gene            complement(685..>1741)
                     /locus_tag="ACZPE2_04725"
     CDS             complement(685..>1741)
                     /locus_tag="ACZPE2_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319186.1"
                     /translation="LGPVWGGIIDLFGKKNSLLIALLTFAASYAAIYFFPSFPCFVVA
                     LPISYAATLTFDSAMYSYLSEMIPYEGRGRMTAVLEAAYALGFMIGSPVAGVLMENFG
                     WAVPFVLMAGIAVVFAIALGFILPKEGEKRFGIDATVSHEMQENKAEKGSYFHRLSLV
                     LKDKNALAALLTGSLLMGGNWFCQTMYGGWLEDNFDQGLSEIGFISAAFGLAVFVGSL
                     LCLTLTDKLGKTKAMKLGAIVTICGGVIAAFTNQKSLIIGSIGLFLYFAFVEFATEAC
                     ISLCTDVRAEIRGTVIGSSFGALAIGSTIGTGVSPVLYTKFGYTPCIIVGTVIVAIAL
                     LVIAKVLTPGFANKEKN"
BASE COUNT          489 a          438 c          360 g          454 t
ORIGIN      
        1 tttgcggtcc gtgtctgaaa tcataaaaag ctcccgccca atccatacac agtctgacag
       61 cttcaaaaac aagcttgctg tcatcggtat ctattcctcg gttaagtgcc tctccgcagc
      121 attcgaggaa cctgttactg tcatcgttcg tctcaccgga gaacaattcc ggactcatcc
      181 agctcacatg gctttcgggg tccacctcgt atgaccagtg atactcatcg gcaacacctt
      241 caagatactt gtaaagatat tcggaaaaag cctttacaat tttcttgttt gacatcggtt
      301 ctttatcctg agcggctgtc tccgcttggc cgtaaagcac ccgattttca acagttattg
      361 catcaagctg catagcttct tttccctttt caaaggacag gccgccgcct gatgcaacac
      421 tgtatctcgc aagcatcaaa tatcccgcgt gttcaaaaaa gtgtgagacg ctctttgccg
      481 tttcggcctc atacctttct gcaactttga tcaattcttc gtcaatcgtt attgttatat
      541 tcttcttcac atttatactc ccctatgcat tttattaata agactatcca tatgtattat
      601 attattaatt ctttattact gtcaaccgtt acggcaaaca aatatccgac agacatcgaa
      661 cacagggaaa cgctaaatgt ataatcagtt tttctcctta ttggcaaaac cgggagtcag
      721 aaccttagca ataacaagca aagctatggc cacaataacc gtaccgacga tgatacacgg
      781 agtatatccg aacttcgtat aaagaacggg tgacacaccg gttcctatcg tgctgcctat
      841 cgcaagcgcg ccgaagcttg aacctattac ggttccgcgt atttcagctc ttacatccgt
      901 gcacagagaa atacaagcct ctgttgcaaa ttcgacgaat gcaaagtaaa ggaaaagacc
      961 gatgcttccg ataataagcg atttttggtt ggtgaatgcg gctattacgc ctccgcatat
     1021 agtcacaatg gcaccgagct tcatggcttt tgtttttccg agcttgtctg taagagtaag
     1081 gcaaaggagc gagccaacaa ataccgctaa tccgaatgcc gcggaaataa agccgatctc
     1141 cgataagccc tggtcgaaat tatcttcaag ccaaccgccg tacattgtct ggcagaacca
     1201 gttaccgccc ataagaagcg agccggtaag aagggctgcc aaggcatttt tatccttgag
     1261 tacgagcgaa agtctgtgga aatatgagcc tttttccgcc ttgttctctt gcatctcgtg
     1321 agaaacggtt gcgtcgatac cgaaccgttt ttctccttcc ttcgggagaa taaagcctaa
     1381 agcgattgca aaaaccacgg cgatgcctgc cattaagaca aacggtacag cccagccgaa
     1441 gttttccata agaacacctg caaccggtga gcctatcata aagccgagtg catatgcggc
     1501 ttcaagaacc gcagtcatac ggcctctgcc ctcgtaagga atcatttcgc tgaggtaaga
     1561 gtacattgcc gagtcaaaag tgagtgtcgc ggcataggaa atgggtaacg cgacaacaaa
     1621 gcaagggaag cttgggaaga agtatatagc cgcatagctt gccgcaaatg tgagcaatgc
     1681 tattaaaagc gaattcttct ttccgaacaa gtcaataata ccgccccata caggtcctaa
     1741 a
//
LOCUS       DBMDVO010000286         1740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_32893_length_1740_cov_1.125686, whole genome shotgun sequence.
ACCESSION   DBMDVO010000286 DBMDVO010000000
VERSION     DBMDVO010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32893_length_1740_cov_1.125686"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="ACZPE2_04730"
     CDS             complement(<1..540)
                     /locus_tag="ACZPE2_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012951.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR1319187.1"
                     /translation="MTDNQKLAELLFPEITKTPEDYEKLYPERNLKEGARVTRFAPSP
                     TGYLHLGGFFGALTDYLTAKASGGIVYLRIEDTDKKREIADGVSAIINGFKSFGITFD
                     EGVTGIDSEKGDYGPYTQSKREEIYKTVAKSLVLKGLAYPCFCTAEELTALRDRQEKD
                     GALIRGYFGKYAKCRNLTYE"
     gene            complement(602..>1740)
                     /gene="murA"
                     /locus_tag="ACZPE2_04735"
     CDS             complement(602..>1740)
                     /gene="murA"
                     /locus_tag="ACZPE2_04735"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1319188.1"
                     /translation="LNILSNMGASIKFINNSTIEIDTRHINSFVVEHELSKQLRASYY
                     LIGALLGRFNKAKVAMPGGCYLGVRPIDQHIKGFEALGAEVTMEENAMVNARAESLLG
                     NAVYLDVVSVGATINVMLAAVKAKGLTIIENAAKEPHIVDLANFLNTMGADVRGAGTD
                     VIKIRGVEHLHGCEYSIIPDQIEAGTYMVAAAATRGDVLIKNVIPKHLESISAKLIEC
                     GVTVTEYDDSVRVSCDRRPGKCNIKTMPHPGFPTDMQPQFAVLLCLADGTSIISESVW
                     DNRFRYAEQLTRMGAKIHVDGKTAIIEGVPSLKGAPIRADDLRAGAAMMIAGMVATGV
                     TEIEEIYHIERGYENVVEKFRSLGAVIERKTFPDEDKKSHKNAG"
BASE COUNT          392 a          450 c          413 g          485 t
ORIGIN      
        1 ttcgtaggtt aaattacggc actttgcata tttgccgaaa taacctctta taagagcacc
       61 gtccttttcc tgcctgtcgc gaagagccgt aagctcctcc gccgtgcaga agcaagggta
      121 tgcaagtccc tttaagacaa gactttttgc tactgtttta taaatctctt cacgcttgct
      181 ctgcgtgtaa gggccgtagt cgcccttttc gctgtctatg ccggtaacgc cctcgtcaaa
      241 cgtaatgccg aacgacttaa agccgtttat gatggcggaa acgccgtcgg caatttctct
      301 ctttttgtcg gtatcctcaa ttctcagata cactattccg cccgaggcct ttgccgtcaa
      361 atagtccgtc agcgcgccga aaaagccgcc gaggtgaagg tagcccgtgg ggctcggtgc
      421 aaatcgcgtg acgcgcgcac cctccttcaa atttctttcc ggatagagct tttcgtagtc
      481 ctccggtgtt tttgtgattt cggggaacaa aagctccgcg agtttttgat tgtcagtcat
      541 atcggtacac ccctgaataa aagcaaaaag ccgctctcgg cggcttaata ttaggataac
      601 attaaccggc gtttttatga gattttttat cttcgtccgg gaaggtcttt ctctcaataa
      661 cagcgccgag cgagcggaat ttttcaacga cattttcata tccgcgctct atgtgataaa
      721 tttcttcaat ctctgtaacg cccgtagcga ccatgcctgc tatcatcata gccgcgcctg
      781 cgcgaaggtc gtccgctctt ataggtgcac ccttaagcga cggaacgccc tcgataatag
      841 ccgtttttcc gtcgacgtgt attttggcac ccattcttgt gagctgctcc gcatagcgga
      901 aacgattgtc ccaaacgctc tcggaaataa tgcttgtacc gtcagccagg cataaaagca
      961 cggcgaactg cggctgcata tcggtcggaa atcccgggtg aggcatggtt ttgatgttgc
     1021 atttacccgg tctgcggtcg catgaaactc ttacggagtc atcatattcg gtgacggtga
     1081 cgccgcattc tataagcttt gccgaaatgc tctcgagatg cttcggaata acgtttttta
     1141 taagcacgtc acctcttgtg gcggctgccg caaccatata ggttccggct tcaatctggt
     1201 cgggaattat ggaatattcg cagccgtgca ggtgctctac gccgcgaatt ttaataacgt
     1261 ccgtgcctgc gcctctgacg tcggcaccca tagtgttaag aaagtttgcg aggtctacta
     1321 tgtgaggctc tttcgctgcg ttttcgatta ttgtaagacc ctttgccttt actgctgcca
     1381 gcatcacgtt tatggtagcg ccgaccgaca caacgtcaag gtaaacagcg ttgcccaaaa
     1441 gagactcggc tcttgcgttg accatcgcgt tttcctccat ggtcacctcg gcaccgagag
     1501 cctcaaagcc ctttatatgc tggtcaatgg ggcgaactcc gagatagcag ccgccgggca
     1561 ttgcaacctt ggccttgtta aatctgccga gaagtgcgcc tataaggtaa tatgacgcgc
     1621 gaagctgttt tgaaagctcg tgctctacta caaatgaatt tatgtgacgc gtatctattt
     1681 caatagtgga attgttgatg aatttaattg acgcacccat attgctcaaa atgttgagca
//
LOCUS       DBMDVO010000287         1732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33092_length_1732_cov_0.818516, whole genome shotgun sequence.
ACCESSION   DBMDVO010000287 DBMDVO010000000
VERSION     DBMDVO010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33092_length_1732_cov_0.818516"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..1127
                     /locus_tag="ACZPE2_04740"
     CDS             60..1127
                     /locus_tag="ACZPE2_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319189.1"
                     /translation="MEEAAKLAGAHEFILQMEKGYDTYVGERGVKLSGGQKQRLITAS
                     TLAMEQKIIILDEPLANLDTHTAHILLKALRNLANSGYAVLIVEHRLDVVKNYIDKVM
                     RIENKQLFTSTDINDLNSGIKTIPHADGSLPGEVLIKGEKLFFAAGDRNIIDGLDIEI
                     RAGERIVLLGENGCGKTTLMRMLARLNKPNDGMLSQTITKSKKANSKWFSRVGYVYQN
                     PVYQLFMPTLLSEISFKAKSEDMAREMINAFGLSGLEQRHPQSLSEGQKRRASIAAVC
                     ASEPTVLFLDEPTVGQDYKNLCKTVETVNKINKNLGTAIVTVTHDKRCAGALADRVLI
                     MENGKISRQGDYRLANEFLDK"
     gene            1149..>1732
                     /locus_tag="ACZPE2_04745"
     CDS             1149..>1732
                     /locus_tag="ACZPE2_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1319190.1"
                     /translation="MIKTRLIEQVPSSKKYIALTVLAQWLKTVANIVMIFILSNLLAQ
                     ILDGKAFDFKGLLPYLVAIAAVMLVRYLCGYASSQSSFFASSEVKKVLRQKMYKKLTR
                     MGASYSEKVSTSEVLQVFVEGVDQLELYFGKYLPQFFFAMLAPITLFAVLVFVSWKAS
                     VVLLICVPLIPLSIVAVQKIAKRLLSKYWGVYTNL"
BASE COUNT          535 a          341 c          395 g          461 t
ORIGIN      
        1 tctctggttc tatttttgaa aatattgctt atgggcgccc tggggcgtcc caaaagcaag
       61 tggaggaagc ggcaaagctg gccggagccc atgagttcat tttacaaatg gaaaaagggt
      121 atgatactta cgtgggggag cgaggcgtta agctttccgg tggtcaaaag caacgcctga
      181 taaccgcttc tacccttgcg atggagcaaa aaatcattat tcttgacgaa ccgcttgcaa
      241 atttggacac tcatacagca cacattttgt tgaaagcact tcgtaatttg gcaaacagcg
      301 gatatgccgt tttaatagtt gagcacaggc ttgatgttgt gaaaaactac atagataaag
      361 ttatgcgaat cgaaaacaaa cagcttttta caagtacgga cataaacgac ttgaacagcg
      421 gaataaaaac aattcctcat gccgacggct ctttgcccgg cgaagtgctt ataaaaggcg
      481 aaaaactctt ttttgccgca ggtgacagaa atattataga tggacttgat atagaaattc
      541 gggcaggcga acgaatagtt ttgctcggtg aaaacggatg cggaaaaacc acccttatgc
      601 gcatgcttgc aagactgaat aagccgaatg acggtatgct cagccaaaca ataacaaaaa
      661 gcaaaaaagc aaacagcaaa tggttttcaa gggtgggata tgtttaccaa aatccggtct
      721 atcagctttt tatgccaacg cttttaagcg agatttcctt taaggcaaaa agcgaggata
      781 tggcaaggga aatgataaat gcctttggac tttcgggact tgagcagaga catccgcaat
      841 cactttccga agggcaaaaa cggcgtgcct ccattgctgc cgtctgtgca agcgagccga
      901 ccgtactgtt tcttgacgag ccgacagtgg gacaggatta caaaaacctt tgtaaaacgg
      961 ttgaaaccgt aaataaaata aacaagaacc tcggcacggc aattgttacc gtaacacacg
     1021 ataaacgatg tgcaggtgct cttgcagaca gagtgctgat tatggaaaac ggtaaaatat
     1081 cacggcaagg cgattaccgc ctcgcaaatg aatttttgga taaataactt aattgatgga
     1141 gaaaagaaat gattaaaaca agacttatag agcaagtacc ttcttcaaag aagtacattg
     1201 ccctaacagt tcttgcgcag tggttgaaaa cggttgcaaa catcgttatg attttcattc
     1261 tttcaaatct tcttgcgcaa attctcgacg gtaaagcgtt tgattttaaa ggacttttgc
     1321 cttatttggt cgcaattgcg gcggttatgc ttgtacgcta tctgtgcgga tacgcaagtt
     1381 cacagagttc gtttttcgct tcaagcgaag taaaaaaagt gcttagacaa aaaatgtata
     1441 aaaagcttac acgaatggga gcatcttaca gcgaaaaggt atcaacatca gaggtacttc
     1501 aggtttttgt tgagggtgta gaccagcttg aactttattt cggcaaatat ctgccgcagt
     1561 ttttctttgc tatgcttgca ccgataacgc tttttgccgt gcttgtgttc gtaagctgga
     1621 aagcgtcggt ggtgcttctt atttgtgtac cccttattcc gctttctatt gttgcagttc
     1681 aaaaaatcgc aaaaagactt ctcagcaaat attggggagt atacacaaac ct
//
LOCUS       DBMDVO010000288         1729 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33164_length_1729_cov_0.791769, whole genome shotgun sequence.
ACCESSION   DBMDVO010000288 DBMDVO010000000
VERSION     DBMDVO010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33164_length_1729_cov_0.791769"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(21..>314)
                     /locus_tag="ACZPE2_04750"
     CDS             complement(21..>314)
                     /locus_tag="ACZPE2_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319191.1"
                     /translation="SSGQKQLLTIARAMLSDPKILILDEATSNVDTMTEQRIQKAFLK
                     MMEGRTSFIIAHRLSTIREADLILVMDKGRIIEQGTHNELLAKNGFYTKLYNS"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<325..1698)
                     /locus_tag="ACZPE2_04755"
     CDS             complement(<325..1698)
                     /locus_tag="ACZPE2_04755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319192.1"
                     /translation="MVAKIIGILFAVYVLNALFTWGMTVFTNALSNHSIEKMRKDAFG
                     KISKLPLKFFDGHSHGDIISRLTNDIDAVSEGLLQGITQLFSGIVTVVGSLVLMFLLD
                     WRITLCVIVITIICIFVSKAIATNSGKMFRLQAQTIGELNGYVSETVGNLKVVKAFGY
                     EDKSSEVFGEINARLYDCGQKAQFYSSLVNPTTRYINNLAYISVGVLGGLAALAGHLS
                     VGIISSFLIYATQFARPINDMTSILTQLQSAQAAAARIFALGEIEPETPDEDRPELEV
                     KNGEVMFKDVDFSYNKDKELIKDLNIVAKPGQRVAIVGPTGAGKTTIVNLLMNFYGVD
                     KGTIFVDGQAIDSVQRDSLRKNFGMVLQDTWLFAGTVKENIAYGKEGATDEEIINAAK
                     AASAHGFIKRLPEGYDTVIAEDGGNLSQGQKQLLCIARVMLCLPPMLILDEATSSIDT
                     RTEIKIQN"
BASE COUNT          496 a          400 c          316 g          507 t
ORIGIN      
        1 tttcacgcac accaaataaa ttagctgtta tatagttttg tataaaatcc attctttgca
       61 agcaattcat tgtgagtacc ctgttcaata attctgccct tgtccataac aagaattaaa
      121 tcagcctcac gaattgttga aagacggtga gcgataataa agcttgttct gccttccatc
      181 atctttagga atgccttttg tattctctgc tccgtcattg tatcgacatt tgaagttgcc
      241 tcgtcaagaa taagtatttt aggatctgac agcatcgcac gcgcaatagt caaaagctgt
      301 ttttgaccgc tcgannnnnn nnnnattctg aatcttgatc tcagtacgcg tatcaatcga
      361 ggatgtcgcc tcatcaagaa tcagcatcgg aggcaggcac agcatgactc ttgcaatgca
      421 taacagctgc ttctgtccct gtgacaggtt gccgccatcc tctgcgatca cggtgtcata
      481 tccctccggc agacgcttga taaagccgtg tgcagacgca gcctttgcgg catttataat
      541 ttcttcgtct gtcgcaccct ctttaccgta tgcgatattt tcttttacag tacccgcaaa
      601 caaccaagta tcttgaagta ccataccgaa attcttacgc aagctgtcac gctgaacaga
      661 atcaatagcc tgtccgtcaa cgaatatagt acccttatcc acaccgtaga agttcatcaa
      721 aagatttacg atagttgttt tacctgcacc cgtaggtccg actattgcta ctctttgacc
      781 cggttttgca acaatattca agtctttgat aagttcttta tccttattat atgagaaatc
      841 aacgtcttta aacataactt caccgttttt aacttcaagt tcgggtctgt cctcgtcagg
      901 tgtttcaggc tcaatttcgc caagtgcgaa aattcttgcc gccgctgcct gtgcagattg
      961 aagttgagtt aaaatacttg tcatatcgtt aatcggacgt gcaaactgtg tagcataaat
     1021 caagaaactt gatattatac ctaccgacaa atgtccggca agtgcggcaa gtccgccaag
     1081 aacgccgaca gaaatatacg caaggttatt gatatatctt gtagtcggat tgacaagcga
     1141 tgaatagaat tgagcctttt gaccgcagtc gtaaagacgt gcgtttatct cgccgaatac
     1201 ttcactgctc ttgtcctcat aaccgaatgc cttgacaacc tttaaattgc cgaccgtttc
     1261 cgaaacataa ccgttaagtt caccgatagt ctgtgcctgt aatctgaaca ttttacccga
     1321 attagtcgca atcgcttttg aaacgaatat acaaataatc gtaataacaa taacacaaag
     1381 tgttattctc cagtcaagca agaacatcaa gacaagcgaa ccgacaaccg ttacgatgcc
     1441 cgaaaacagc tgtgtaattc cctgcaaaag gccttcggaa actgcatcta tgtcgtttgt
     1501 aagacggctg ataatatcgc cgtgtgaatg accgtcaaaa aatttaagcg gcaattttga
     1561 aatcttgccg aatgcgtctt tacgcatttt ttcaatcgaa tggtttgaaa gtgcgtttgt
     1621 aaacactgtc attccccatg taaacaaagc attaagcaca taaaccgcaa aaagtattcc
     1681 tataattttc gcaaccatag gaaaatctac gttattttcg cctttgata
//
LOCUS       DBMDVO010000289         1723 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33318_length_1723_cov_1.879778, whole genome shotgun sequence.
ACCESSION   DBMDVO010000289 DBMDVO010000000
VERSION     DBMDVO010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33318_length_1723_cov_1.879778"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1723)
                     /locus_tag="ACZPE2_04760"
     CDS             complement(<1..>1723)
                     /locus_tag="ACZPE2_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR1319193.1"
                     /translation="GEGSSGIAAGAAKVYDKLASALDKSNELTITGCIGMCFLEPIVD
                     IYDNGTLTRLVKVSENDCERIAKAVNSDDLSTVEDLKISEDDELFLKKQTRIALRHCG
                     IINPEKISDYENTDGYKAVRKVLSEMTPEQVIEEIKISGLAGRGGAGFPTWFKWNAAR
                     SSDGTEKYLICNADEGDPGAFMDRAVIEGDPHNLIEGMLIGAYAIGAKEAIVYVRAEY
                     PLAIKRLEKAIKQATEKGYLGNNIFGSGFSCKMRIKAGAGAFVCGEETALIESLEGSR
                     GMPRLKPPFPAQAGYWHKPSNINNVETFANVSWIILNGGAAFSSMGTADSKGTKVFAL
                     TGKIKKGGLVEIPMGKTLRDVIFDIGGGIKSDKKFKAVQMGGPSGGCIPNDLLDTVID
                     YKALGATGAIMGSGGMVVMDESTCMVGMAKFFLDFTAKESCGKCAHCRLGTKRMLEIL
                     TRIVDGKGEKGDIELLEELCYAVKDGALCGLGQTAPNPVLTTLKYFRNEYEAHIKDKK
                     CPAGECTALVTYKIDKNKCVGCTLCARNCPVSAISGTVKKPHEIDSLKCIKCGKCKEN
                     CRFGAIEV"
BASE COUNT          442 a          459 c          325 g          497 t
ORIGIN      
        1 cacctctatc gcaccgaaac gacagttttc tttacattta ccacacttaa tgcatttaag
       61 cgaatctatt tcgtgcggtt ttttcacagt accgcttatt gcagatacag gacagttcct
      121 tgcacataat gtacatccga cacatttatt tttatctatt ttataagtaa ccaacgctgt
      181 gcattcgccg gcagggcatt ttttatcttt aatatgcgct tcatattcat tcctgaaata
      241 tttaagcgtc gtaagcaccg ggttaggcgc agtttgccca aggccgcaaa gcgcaccatc
      301 tttaacggca tagcaaagct cctcaagtag ctctatatcg cctttctccc ctttaccgtc
      361 aactattcgc gtaaggattt caagcatacg ctttgttccg agacggcagt gcgcacattt
      421 accgcatgac tctttagcgg taaaatcaag gaagaatttc gccataccga ccatacacgt
      481 gctctcatcc ataacaacca taccgcccga gcccataatt gccccggtag caccgagtgc
      541 tttataatca ataacggtat caagcaagtc attcggaata catccgccgg aaggaccacc
      601 catctgaacg gctttaaatt ttttatctga tttaattcct ccgccgatgt caaagataac
      661 atcacgtaag gtttttccca tcggaatctc aacaagaccg ccttttttaa ttttacctgt
      721 aagcgcaaat acctttgtac ccttactgtc ggcggttccc atagaggaaa aagcggctcc
      781 gccgtttaat attatccacg aaacattggc aaaggtttca acattattta tattagacgg
      841 cttatgccag taaccggcct gtgccggaaa aggaggcttg aggcgcggca taccgcgtga
      901 accttcaaga gattcaataa gcgcggtttc ttctccgcac acaaaagcac cggctcctgc
      961 cttaattcgc attttacaag aaaatccgct accgaaaata ttgttgccga gatagccttt
     1021 ttcggttgct tgttttatgg ctttttcgag tcgttttata gcaagcggat attccgccct
     1081 tacataaaca attgcttctt tcgcacctat ggcatatgcg cctataagca taccctctat
     1141 caggttatgc ggatcgccct cgataacagc cctgtccata aatgcgcccg ggtcaccctc
     1201 atccgcatta caaatcaaat atttttccgt gccgtccgaa gaacgcgcgg cattccattt
     1261 aaaccacgtc ggaaacccgg caccgccgcg accggcaagg ccggaaattt tgatttcctc
     1321 aatgacctgc tcaggcgtca tttcggaaag aacttttctt acagccttat atccgtctgt
     1381 attttcgtag tcgctgatct tttcaggatt tataatgccg caatgcctca aagcaattcg
     1441 ggtctgcttt tttaaaaaga gctcgtcgtc ctcggatatt ttaaggtcct ctaccgttga
     1501 caaatcatcg gagttaactg cctttgctat tctctcgcag tcgttttccg aaacctttac
     1561 aagccgcgta agagtaccgt tatcgtaaat gtcaacaatc ggctccaaga agcacatacc
     1621 tatacatcct gttatggtaa gttcgttact cttgtccaaa gcagacgcga gcttgtcgta
     1681 gaccttagct gctcctgcag ctataccgga gctgccttct cca
//
LOCUS       DBMDVO010000290         1720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33414_length_1720_cov_0.749846, whole genome shotgun sequence.
ACCESSION   DBMDVO010000290 DBMDVO010000000
VERSION     DBMDVO010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33414_length_1720_cov_0.749846"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="ACZPE2_04765"
     CDS             complement(<1..298)
                     /locus_tag="ACZPE2_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024747.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319194.1"
                     /translation="MKVIEIFDSIDGEGIRTGQCATFIRLAGCNLRCSYCDTVYSLFG
                     EETPCEYTEMTVDEIISKVNMTYKRVTLTGGEPLVHTDSAELVSKLTEIDCDVNI"
     gene            complement(310..666)
                     /gene="queD"
                     /locus_tag="ACZPE2_04770"
     CDS             complement(310..666)
                     /gene="queD"
                     /locus_tag="ACZPE2_04770"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HIR1319195.1"
                     /translation="MYEVKKRFEISAAHKLDLDYDSKCTNFHGHNWIIDVYLQSETLD
                     KNGMVLDFTHIKRKIQDKFDHKVINEVVPFNPTAENLAKYICDELAPFCYRVDVQESQ
                     DNIASYIKDDVKIKLL"
     gene            complement(654..1325)
                     /gene="queC"
                     /locus_tag="ACZPE2_04775"
     CDS             complement(654..1325)
                     /gene="queC"
                     /locus_tag="ACZPE2_04775"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR1319196.1"
                     /translation="MKNRKALVVLSGGQDSTTCLFWAIDKFGKENVSAIGFDYGQRHK
                     LELECADKICKKVGIPYEIVPTPIINQLSANSLTREDIPVEETKPEGAPPNTFVEGRN
                     LLFLSYAAIYAKTHGITDLVTGVCETDFSGYPDCRDIFVKSLNVTINLAMDYNFVIHT
                     PLMWLNKAETWKMADDLGVLDTIYNETLTCYNGVLGEGCGHCPSCTLRRRGYEQYMEM
                     KKCTK"
     gene            complement(1339..>1720)
                     /gene="queF"
                     /locus_tag="ACZPE2_04780"
     CDS             complement(1339..>1720)
                     /gene="queF"
                     /locus_tag="ACZPE2_04780"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR1319197.1"
                     /translation="ETFVNKHQGNDYMVTFDCPEFTSLCPITGQPDFAKITINYIPNV
                     KMVESKSLKLYLFSFRNQGDFHEDCINIIMKDLVKLMEPKYLEVKGIFTPRGGISIYP
                     FANYGQPGTKYEKIAENRLFDSLK"
BASE COUNT          486 a          355 c          316 g          563 t
ORIGIN      
        1 caatattaac atcacagtcg atttcggtca gttttgaaac aagctccgca ctgtcggtat
       61 gaacaagagg ctcaccgcct gtaagcgtaa cacgcttgta agtcatatta acctttgaaa
      121 taatttcatc aacagtcatt tcagtatatt cacatggtgt ttcttcaccg aacagtgaat
      181 aaaccgtatc gcagtacgaa cagcgtaggt tacagcctgc aaggcgtata aatgttgcac
      241 attgtcctgt tcgtatgccc tcgccgtcaa tactgtcaaa aatttcgatt actttcattt
      301 ggattttcct tataacaatt ttattttaac atcatctttg atgtagcttg caatgttgtc
      361 ctgactttcc tgtacgtcaa cacgataaca gaatggtgca agctcatcgc aaatgtattt
      421 cgccaagttt tcggcagtag gattgaacgg tacaacttca ttaataacct tgtggtcaaa
      481 cttgtcttga attttgcgtt tgatgtgtgt aaaatcaaga accataccgt ttttgtcaag
      541 agtttcactt tgaagatata cgtcaattat ccagttgtga ccatgaaaat tcgtgcattt
      601 tgagtcgtag tcaaggtcta acttgtgtgc cgcacttatt tcaaatcttt tctttacttc
      661 gtacattttt tcatctccat atattgttcg taaccgcgtc ttctgagcgt gcatgacgga
      721 cagtgtccgc agccttcgcc gagtacgccg ttataacaag taagtgtttc gttgtaaata
      781 gtgtcaagta cacccaaatc gtcagccatt ttccaagttt cagccttatt cagccacata
      841 agcggtgtgt ggataacaaa attgtaatcc atagcaagat tgattgtaac attaagagat
      901 ttaacgaaaa tatcacgaca atccggataa cccgaaaaat ctgtttcgca tacgcctgtt
      961 acaaggtctg ttatgccgtg agtttttgca tatatagcgg catagcttaa aaacaataag
     1021 tttctgccct caacaaatgt attcggaggt gcaccctccg gctttgtttc ctcaacggga
     1081 atatcttcac gcgtaagtga atttgcggaa agctgattta taataggtgt cggaacgatt
     1141 tcataaggaa taccgacttt tttacaaatc ttgtctgcac attcaagctc aagcttgtga
     1201 cgctgaccgt agtcaaaacc tattgcagat acattttctt taccaaattt gtctattgcc
     1261 cagaacagac aagttgtact gtcctgaccg cccgataaaa caacaagtgc ttttctgttt
     1321 ttcattttaa tcctccattt attttaagct gtcaaacaat ctgttttcag cgattttttc
     1381 atatttagta cccggttgac cgtagtttgc aaacggataa atagatatgc cgccgcgcgg
     1441 cgtgaaaata cctttaactt caagatattt cggctccata agtttaacaa ggtctttcat
     1501 tataatattg atacagtcct cgtgaaaatc accttgattt ctgaaactga aaagatacag
     1561 ctttaatgat tttgattcaa ccattttcac gtttggaata tagttgatgg tgatttttgc
     1621 aaaatccggc tgacctgtga ttggacaaag cgacgtaaat tcgggacagt caaacgttac
     1681 catataatcg ttgccttggt gcttgtttac gaatgtttca
//
LOCUS       DBMDVO010000291         1717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33498_length_1717_cov_0.868193, whole genome shotgun sequence.
ACCESSION   DBMDVO010000291 DBMDVO010000000
VERSION     DBMDVO010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33498_length_1717_cov_0.868193"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="ACZPE2_04785"
     CDS             <1..1259
                     /locus_tag="ACZPE2_04785"
                     /inference="COORDINATES: protein motif:HMM:NF047104.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1319198.1"
                     /translation="RELPSDYFARFEKAKRQTADVSPALERKNSFKEVSMGFTEEQAK
                     AEAMRCLECGCHDFFDCKLIKYANKYNVKPEKFNGTKHSRNNENKSSLIIRNVDKCIL
                     CGLCVRVCDEAMGNTALGLIGRGFDTVVSPEFGLPLEKTDCSFCGQCAVVCPTGAIIE
                     KQPCVKNLTVKEEIVDSVCNLCSALCKTEIHKIGNTVIRIKPSGENGLLCKAGKFSVF
                     ALNDLKAQALTNQSKMLQAVKKIVSEADRSNISVVLGPSVTVEEIKSAFNITDNVFAE
                     LNPSAAAFRAFEKYGVKSVEDMPYKDVCILVKSTFGNVKKGTLISIDFKQNAEADISI
                     ECAPFVSDSGTYIDTDGVKHLNCAVNSALPTALSVMKALVIKNSDEKSEDVHESSAIH
                     KEIIRKGGAYRQNLSDSFVKTFTDNY"
     gene            1297..>1717
                     /locus_tag="ACZPE2_04790"
     CDS             1297..>1717
                     /locus_tag="ACZPE2_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319199.1"
                     /translation="MKRKTSKFFAALISVIVAVSVLTVTVFAADTKGLSSGLKKYLSN
                     PENTQFDFSDTSVVDNDADWTVFVLSRCGEKDVYPEYSEYINNAVKENYASLKPSDLA
                     RITLSVKAYGLDPENIGGKDLISALKSVDYSSQTYMSS"
BASE COUNT          541 a          313 c          380 g          483 t
ORIGIN      
        1 aaagagaatt gccatcggat tattttgccc gatttgaaaa agcaaaacgc cagactgcag
       61 atgtttcacc tgcactcgag aggaaaaaca gctttaagga agtctctatg ggatttaccg
      121 aggagcaggc caaagccgag gcgatgcgct gccttgaatg cggttgccat gatttctttg
      181 attgtaagct tataaagtat gcaaataaat acaatgttaa gccggaaaaa tttaatggaa
      241 cgaagcacag cagaaacaat gaaaacaaat cttcgcttat aataagaaat gttgacaagt
      301 gcattctttg cggactttgc gtcagagtct gtgacgaagc aatgggcaat acggcattag
      361 gtcttatcgg tcgcggattt gatactgttg tttcgcctga gtttggactg ccactcgaaa
      421 aaacggattg ctctttttgc ggtcagtgcg cggttgtttg tccgacgggt gcaattatcg
      481 aaaagcagcc gtgtgtcaaa aaccttaccg ttaaggaaga aatcgtagat tccgtgtgta
      541 atctttgctc cgcactttgc aaaaccgaaa tccacaaaat cggaaatact gtaattcgaa
      601 ttaaaccgtc aggagaaaac ggtctactgt gcaaagcggg taagttctct gtgtttgccc
      661 ttaatgactt aaaagcccag gcgttaacta accaaagcaa aatgctgcaa gcggtcaaaa
      721 agattgtttc ggaggctgac agaagtaata tttcggttgt tctcggtcca tctgtcaccg
      781 tggaagaaat caagtctgca tttaatatta cagataatgt ttttgcagag cttaatccat
      841 ctgccgctgc attcagagct tttgaaaaat acggagtaaa atcggttgag gatatgcctt
      901 ataaagatgt atgtattctt gtaaagagca cattcggcaa tgttaaaaaa ggcactttaa
      961 tttctattga ctttaaacaa aatgcggaag ccgatatttc aatagagtgc gcaccttttg
     1021 tctccgacag cggaacatat attgatactg atggtgttaa gcatttgaat tgtgcggtaa
     1081 acagtgctct accgacggct ttgtcggtta tgaaggcact tgtaatcaaa aactctgatg
     1141 aaaaatccga agatgttcat gaaagctcag cgattcacaa agaaataatc aggaagggcg
     1201 gagcgtacag gcagaaccta agcgattcgt ttgtaaagac ttttacagac aattactaaa
     1261 atatttatct gtgagattta taaaggagtt ttcactatga aaagaaaaac aagtaaattt
     1321 tttgcagcgt taatatcggt aattgttgcc gtttcggtat tgactgttac tgtttttgct
     1381 gcggacacaa agggcctttc gtcgggcctt aaaaaatatc tttcaaatcc cgaaaacaca
     1441 caatttgatt tttcggatac ctcggttgtt gacaatgacg ccgattggac tgtttttgta
     1501 ctttcaagat gcggcgaaaa agatgtatat ccggaatatt cggaatacat aaataacgca
     1561 gtcaaagaaa attatgcgtc attaaagccg agtgatttag caagaattac gctctcggtt
     1621 aaagcgtatg gattggatcc tgaaaatatc ggaggaaaag acctcatttc cgcactgaag
     1681 tcagttgatt attcctctca aacctatatg agcagca
//
LOCUS       DBMDVO010000292         1702 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33871_length_1702_cov_1.467208, whole genome shotgun sequence.
ACCESSION   DBMDVO010000292 DBMDVO010000000
VERSION     DBMDVO010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33871_length_1702_cov_1.467208"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="ACZPE2_04795"
     CDS             complement(<1..562)
                     /locus_tag="ACZPE2_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1319200.1"
                     /translation="MDYMETREVMCDICHKMWQLGWVAANDGNLSVKLPDGNFLATPT
                     GISKSFITPEKLVIINPEGEVLQAIDGYRPSSEIKMHLRCYKERDDVGAVLHAHPPTA
                     TGYAVAHIPLDRYTMIETVAAIGSIPVTPYGTPSTYEVPDAIAPYLQEHDVLLLANHG
                     ALTVGADAITAYYRMETLELFAKISLN"
     gene            complement(588..1022)
                     /locus_tag="ACZPE2_04800"
     CDS             complement(588..1022)
                     /locus_tag="ACZPE2_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR1319201.1"
                     /translation="MLKGIPEILSPELLKVLCEMGHSDRLVIADGNFPVMSMGKNAQI
                     IRMDGHGVPEILDAILRVFPLDTYIEKPVNLMEVMPGDDVQTPIWDVYKKTVEKYDER
                     GKNAIGEIERFKFYEETKTAYAVIATSEKALYANIMLQKGVV"
     gene            complement(1015..>1702)
                     /locus_tag="ACZPE2_04805"
     CDS             complement(1015..>1702)
                     /locus_tag="ACZPE2_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1319202.1"
                     /translation="LLGTELLKPLINEKTLRCNVTNEGGYGGKASFLKNIIGLWLIQE
                     TRRQWQKEGDSLSFAEMETLARNAKPFSCFIDPDAPDFVAAGNIPERIRAYCRRTGQY
                     VPESKGEILRCIYESLAMKYRYAAEQIEFCTGKEYDTIYIVGGGTKDKLLSSFTASAC
                     NKNVSAGPTEATVLGNIALQLLAEGKIKTLRDARRIISNSEEVTSFTPSEPEAWNKKY
                     QTFKEVVSLA"
BASE COUNT          393 a          426 c          395 g          488 t
ORIGIN      
        1 cattaagcga aatctttgca aaaagttcaa gcgtttccat acggtaatat gcggttattg
       61 cgtcggcacc gaccgtaagc gcgccgtgat ttgcaagaag caatacatcg tgctcttgaa
      121 gatacggggc aatagcgtcc ggcacctcgt atgtagacgg cgtaccgtac ggtgttacgg
      181 gaatcgagcc tatcgccgca acggtttcta tcatcgtgta tctgtcaagc ggaatgtgcg
      241 ctacggcgta tcccgtggct gtaggcggat gagcatggag caccgctcct acgtcgtcgc
      301 gttctttata gcagcgaaga tgcattttga tttccgacga cggtctgtaa ccgtcaatag
      361 cctggagcac ctcgccctcg ggatttatta taacaagctt ttcgggggtt atgaagcttt
      421 tgctgatgcc tgtcggcgtt gcaaggaagt taccgtcggg caattttacc gagaggttac
      481 cgtcatttgc cgcaacccag ccgagctgcc acatcttatg gcaaatatcg cacataactt
      541 ctcttgtttc catataatcc atttttctag ttcctttcgt tatttaatca gacaacgccc
      601 ttttggagca ttatattggc ataaagagcc ttttcgcttg tagctataac agcatacgcc
      661 gtctttgtct cttcgtaaaa tttgaatcgc tcgatttcgc ctatggcgtt tttcccgcgc
      721 tcatcatatt tttcgactgt ttttttgtaa acgtcccaaa tcggagtctg tacatcgtcg
      781 cccggcataa cctccataag atttaccggc ttttcgatat acgtatcaag cggaaacact
      841 cttagaattg catcaagtat ttccggtacg ccgtgcccgt ccatacggat tatctgagcg
      901 tttttgccca tactcataac ggggaaattg ccgtccgcga taacaagacg gtcgctgtga
      961 cccatttcac agagcacctt taaaagctcc ggagaaagta tttccggtat tcctttaagc
     1021 aagagaaact acctccttaa aagtctgata ttttttattc cacgcttcgg gttcgcttgg
     1081 tgtaaagctt gtgacctctt cggaattact gattattctg cgcgcgtcac ggagcgtttt
     1141 tattttgccc tccgccaaaa gctgaagagc aatattgccg agaacggtgg cctcggtagg
     1201 tccggcggaa acatttttat tgcacgccga cgcggtaaac gatgagagaa gcttatcctt
     1261 tgtgccgccg cccacaatat atattgtgtc gtattctttg ccggtgcaga actcaatctg
     1321 ctctgcggca taacggtatt tcatggcaag gctctcataa atacagcgga gaatttctcc
     1381 tttggattcg ggaacatact gccctgttct gcgacaatat gcccttatcc gctccggaat
     1441 attcccggcg gcaacaaaat cgggagcgtc ggggtctata aagcaagaaa acggcttcgc
     1501 gtttcgtgcg agagtctcca tctcggcaaa cgaaaggctg tcgccctcct tctgccactg
     1561 tcggcgagtc tcctgtataa gccaaagacc gataatattt ttaaggaaag acgcttttcc
     1621 gccgtatccg ccctcgtttg taacattgca gcggagcgtt ttttcgttta tgagaggctt
     1681 taagagctct gttccgagca gt
//
LOCUS       DBMDVO010000293         1700 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_33936_length_1700_cov_1.223890, whole genome shotgun sequence.
ACCESSION   DBMDVO010000293 DBMDVO010000000
VERSION     DBMDVO010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33936_length_1700_cov_1.223890"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="ACZPE2_04810"
     CDS             complement(<1..138)
                     /locus_tag="ACZPE2_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319203.1"
                     /translation="MLEAVNVGLQYGGRTLFEHADVKFTSGNCYGLIGANGAGKSTFL
                     KI"
     gene            complement(223..>1700)
                     /locus_tag="ACZPE2_04815"
     CDS             complement(223..>1700)
                     /locus_tag="ACZPE2_04815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1319204.1"
                     /translation="CKKICFRQGVNIMKFLQKTQRTVMLLLKVLLFFILFATFFVFFG
                     IENEWLLRLSRTAAVTMLTFVVLGSALMSIYGGYSVGITKSKPIIYSMTLSTVFTDIV
                     THLQLCIMNTNAANNQKFQYEHPLLLLLVMVIQILVIIFFAYFGNFVYFSINSPEKCC
                     VITTSKYSLNNIIPKIKKYKKQYLITDAILFTNPDLFDIINRCDTVFVYDVPAASRTT
                     IVEYCYANNKNIYYNFEMCDVVSLRGKNAMLDDKPMIASTVKGLTVEQRIMKRAMDLF
                     VSTVGLIITSPIMLACAIAIKAEDGGSVFFKQKRATKDGKIFKVYKFRTMKEKNSINK
                     SVTEDDDRITKVGKILRKFRIDELPQIINIFKGEMSVVGPRPEMIENVDEYTQALPEF
                     SYRLRVKAGLTGLAQIAGKYNTSPKDKLVLDLMYIEKYSIWQDIKLIFQTLTVFFKAD
                     DSTEAFHHSDSYEFFTADESPELIGEPENAESNVCEQENKQ"
BASE COUNT          443 a          359 c          375 g          523 t
ORIGIN      
        1 aatttttaaa aatgtggatt tgccggcgcc gttcgcaccg ataagaccgt agcagttgcc
       61 gcttgtgaac ttcacgtccg cgtgctcaaa aagcgtcctg ccgccgtatt gtaaacctac
      121 gtttaccgct tctaacatct tttgcttcac ctcatatatt tactcataaa aaaccgcctt
      181 agcgtcacac aaaacagcgc aagacggttt ttaatttaaa ttttattgct tgttttcttg
      241 ctcgcagaca tttgattctg cgttttcggg ttcgcctata agctctggag actcgtccgc
      301 ggtgaaaaac tcgtagctgt cgctgtggtg gaatgcctct gtgctgtcgt cggctttaaa
      361 gaataccgta agcgtctgga agataagctt aatatcctgc catatgctgt atttctcgat
      421 gtacataagg tcgagcacga gcttgtcctt aggcgaagtg ttgtatttgc cggcgatctg
      481 tgcaagaccc gtaagacctg ccttgacgcg caaacggtag ctgaattccg gcagcgcctg
      541 tgtgtattcg tctacatttt ctatcatttc cggacgtgga ccgacaacgc tcatttcacc
      601 cttgaaaatg ttgattatct gcggcagctc gtctatgcgg aatttgcgca gaattttgcc
      661 gacctttgta attctgtcgt cgtcctcggt tacggatttg tttattgagt tcttttcttt
      721 catcgtgcgg aatttgtaga ccttgaaaat ctttccgtcc tttgtggcac gtttctgctt
      781 gaagaatacc gaaccgccgt cctcagcctt tatggctatt gcgcaggcga gcattatggg
      841 gcttgtaata attaaaccca cggtagatac aaaaaggtcc atggcacgtt tcattatgcg
      901 ctgttcaacc gtaagccctt taacggttga agctatcatc ggcttgtcgt caagcattgc
      961 gtttttgccg cgaagcgaaa caacgtcgca catctcaaaa ttatagtaga tgtttttgtt
     1021 gtttgcgtag cagtattcga caatcgttgt gcgtgaggct gccggtacgt catatacaaa
     1081 taccgtgtca cagcggttta tgatgtcaaa caaatccgga tttgtgaaaa gtatggcgtc
     1141 cgttattaaa tactgctttt tatacttctt gattttaggg atgatgttgt ttaacgaata
     1201 ctttgaagtc gttataacac agcatttttc gggagagttt attgaaaagt atacgaaatt
     1261 tccgaagtat gcgaaaaata tgataacaag tatctgaatg accataacaa gcagtaaaag
     1321 cagggggtgc tcatactgga atttttggtt gtttgccgca tttgtgttca ttatgcaaag
     1381 ctgcaaatga gttacaatat cggtaaatac ggtggagagc gtcatagagt agattatagg
     1441 cttgctcttt gttattccca cggaatatcc gccgtatatt gacataagtg ccgagccgag
     1501 aacgacaaac gtaagcatgg ttaccgcggc ggttctcgaa aggcgcagca gccattcgtt
     1561 ttcaataccg aaaaaaacaa aaaaagtggc aaacaaaata aagaatagca gtacctttaa
     1621 gagaagcata accgtccttt gggttttctg taaaaacttc attatattta ctccctgtcg
     1681 gaaacatatt tttttacaca
//
LOCUS       DBMDVO010000294         1695 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34057_length_1695_cov_1.515684, whole genome shotgun sequence.
ACCESSION   DBMDVO010000294 DBMDVO010000000
VERSION     DBMDVO010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34057_length_1695_cov_1.515684"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="ACZPE2_04820"
     CDS             complement(<1..760)
                     /locus_tag="ACZPE2_04820"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HIR1319205.1"
                     /translation="MCGIVGYVGAKECTAILVNSLTKLEYRGYDSAGIAVFEGDRIKT
                     VKAKGKLKEGLIKKLENEPHFTATAGIGHTRWATHGEPSDINSHPIGNGRVSIVHNGI
                     IENYRKLKEFLISKGYGFESQTDTEAVAKLLDYNYDGDPIDTIIRTIADIEGAYSLGI
                     MFREHKNRIFAARKESPLIVGKGNGEMFIASDVTAIIEYTREYYLLEPGEVADITADG
                     VTFYDMHKNVIEKELQVATWDVSAAEKGGYAHFML"
     gene            1039..>1695
                     /locus_tag="ACZPE2_04825"
     CDS             1039..>1695
                     /locus_tag="ACZPE2_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1319206.1"
                     /translation="MGLGLQNVQELTGYIEEMFSEYMTFPTALFIVAIVVALSSIACF
                     ILAPFYIKGKARGAKTAAQVISVIDFIIGILLILAFIIITLLFNMASDGFFDSADEFL
                     KAVLGFLIVPTRFGMSLEAVFGADANIGTPILFYALYFVTAGLLGLAAFIVSCIARGK
                     FNAANGLIRGAAAPAGAPQYNPYQPYGQPNIPQGYPQQYGQNIPQGYPQQYGQNIPQG
                     Y"
BASE COUNT          417 a          377 c          362 g          539 t
ORIGIN      
        1 tgagcataaa atgagcatat ccgccctttt cagccgccga aacatcccag gtagcaacct
       61 gtaattcctt ttctatgaca ttcttgtgca tatcatagaa cgtaacgccg tcggctgtta
      121 tatccgcaac ctctcccggc tcaagcagat agtattctct tgtgtattcg attatagcgg
      181 taacatcgga cgcaataaac atttcgccat ttcccttgcc gactatcaga gggctttctt
      241 ttcttgccgc aaatatgcgg ttcttatgct ccctgaacat tatgccgagc gaatatgcac
      301 cctctatatc ggcaattgtc ctgattattg tatctatagg gtcgccgtca tagttgtagt
      361 cgagcagctt tgccacagcc tccgtgtcgg tctggctctc aaagccgtat cccttcgata
      421 tgagaaattc cttcagcttg cggtagtttt cgattatacc gttatgaacg attgatactc
      481 tgccgtttcc tatcgggtga gagttaatgt cgctcggctc gccgtgcgtt gcccagcgtg
      541 tatgacctat acctgccgta gctgtgaaat gaggctcgtt ttcgagcttt ttgataagcc
      601 cttccttgag cttgcccttt gccttgacgg tttttattct gtcgccctca aacacggcta
      661 tacctgccga gtcataaccc ctgtattcca gcttcgtaag tgagtttaca agtattgcgg
      721 tgcattcttt tgcgcctaca tatcctacta ttccacacat gattttgttc cgcctttctt
      781 taatcggatg agcagccata ctgctctgta atagtaataa tcatattctg tttgttattc
      841 attaagtaca tttatctgta agccgacagt ctgtgtgacg gcttatataa aaacagtata
      901 tcacattaag ctccaaaagg caatatattt tgtgttgact tgagttatca actgtgttaa
      961 aatggtgttg taaatcacat ttaatgtgat gaaataattt acggaaaata cagttatccg
     1021 tcattaagga ggaaaattat ggggttagga ttacagaatg tgcaagagct gacaggctac
     1081 attgaagaaa tgttttcgga atatatgaca tttccgacag ctttattcat agttgccatt
     1141 gtggtggctt tatcgtcaat agcttgcttt attcttgcgc cgttttacat aaaaggaaag
     1201 gcaagaggag caaaaacagc cgcacaggtg atttctgtca ttgattttat catcggcata
     1261 ttactcattt tggcattcat tatcattaca ttgttgttta atatggcgtc ggacggtttc
     1321 tttgattcgg ctgacgaatt tttgaaagca gtgcttggat ttttgatagt tcctacaaga
     1381 ttcggtatgt cgctcgaggc agttttcgga gctgatgcaa atatcggcac gcccatattg
     1441 ttttatgcct tgtattttgt tacggcaggc ttgctcggac tggccgcatt tatcgtaagc
     1501 tgtatagcaa gaggaaaatt caacgccgca aacggtctta taagaggcgc agccgcacct
     1561 gcgggagcac ctcagtataa cccttatcag ccttacggtc agccgaatat tccgcagggt
     1621 tatcctcagc agtacggaca gaatattccg cagggctacc ctcagcagta cggacagaat
     1681 attccgcagg gttat
//
LOCUS       DBMDVO010000295         1694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34093_length_1694_cov_1.412429, whole genome shotgun sequence.
ACCESSION   DBMDVO010000295 DBMDVO010000000
VERSION     DBMDVO010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34093_length_1694_cov_1.412429"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="murC"
                     /locus_tag="ACZPE2_04830"
     CDS             <1..1330
                     /gene="murC"
                     /locus_tag="ACZPE2_04830"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1319207.1"
                     /translation="FIGIGGSGMCPLAEILHSWGYELSGSDNNESDTLDRIRKLGIPV
                     TMGQRAENIKGAEMIVYTAALLKDNPELVAAKASGIPTFERSKLFGAISRMYKNCIGI
                     CGTHGKTTTTSMTTQIMITAKLDPSAVIGGKLPMMGSSGRVGKSENFVCEACEFVDTF
                     LDLSPDVAVILNIDEDHLDYFKTLDNLIKSFHKFASMATKAVIYNGDDANTLKAMEGI
                     SGKDLITFGMAEENDYYPENIAPVHGAYYEFDAMHKGEFLCHIKLRVPGLHNVLNALA
                     ALAASMYSGADAESCRGGLDAFSGAHRRFELLGKYKGVTFVDDYAHHPAELKVTIDAA
                     MEMGYHSVWAVFQPFTYSRTYMLMDDFAKVLCIPDHCVMTEIMGSREVNTYNVYTSQL
                     AAKIPGSVWFNTFEEVADYVVKNAEDGDLVLTLGCGDIYKAAHIMIDKLK"
     gene            complement(1405..>1694)
                     /locus_tag="ACZPE2_04835"
     CDS             complement(1405..>1694)
                     /locus_tag="ACZPE2_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1319208.1"
                     /translation="KAVNANKDTIVFFCHFGVECVMLSHLLNISPVCLWQGFCAAPTS
                     VTTLYTEEREKGIAVWRCSSFGDISHLYAGNEEPAFAARFCEIYDDMSQRH"
BASE COUNT          452 a          379 c          415 g          448 t
ORIGIN      
        1 ctttattggg ataggcggca gcggtatgtg cccgcttgcc gagatacttc acagctgggg
       61 ctatgagctt tccggttcgg acaataacga gagcgatacg cttgacagaa tacgtaagct
      121 cggtatcccc gttaccatgg gtcaaagggc ggaaaatata aaaggcgccg agatgatagt
      181 ttataccgcg gccttgctta aagataatcc ggagcttgta gccgctaagg caagcggaat
      241 accaaccttt gagcgttcaa agcttttcgg cgcaatttcg agaatgtata aaaactgcat
      301 aggcatttgc ggaactcacg gcaagaccac aactacatct atgactacgc agataatgat
      361 aacagcaaag cttgacccgt ctgccgtaat cggcggaaag cttccgatga tgggctcaag
      421 cggcagagtg ggcaagagtg aaaattttgt ctgtgaagcg tgcgaatttg tcgatacatt
      481 ccttgacttg tcacctgatg ttgccgttat actcaatata gacgaggatc atctcgacta
      541 tttcaaaaca cttgataatc tgattaaaag cttccataag ttcgcttcta tggcgacaaa
      601 ggcggtaatt tacaacggcg atgatgccaa cacattaaag gctatggagg gcatttccgg
      661 caaggatctt attaccttcg gcatggctga agaaaatgat tattatcccg aaaatatcgc
      721 tcccgtacac ggtgcttatt atgaatttga cgctatgcac aagggtgaat ttctctgcca
      781 cataaagctc agagttcccg ggcttcacaa tgtccttaat gcacttgcag ccttggcggc
      841 ttcaatgtat tccggcgcgg acgccgagtc ttgccgcggg ggacttgatg cattcagcgg
      901 tgctcacaga cgttttgagc tgctcggcaa atataagggc gttacttttg tagatgacta
      961 cgctcaccac ccggctgagc tcaaggttac cattgacgcc gctatggaga tgggatatca
     1021 cagcgtttgg gcggtgtttc agccgtttac ctattcgcgc acatatatgc tgatggatga
     1081 ctttgcaaag gtactctgca tacccgacca ttgcgttatg accgaaataa tgggttcgcg
     1141 tgaagttaat acctataatg tatacacttc tcagcttgcc gctaaaattc cgggttccgt
     1201 gtggtttaat acatttgagg aggttgccga ttatgttgtt aaaaatgccg aggacggcga
     1261 ccttgtcctg actctcggct gcggtgatat atacaaggcg gctcatatta tgatagataa
     1321 gcttaaataa gttaagataa aatacaatat aaaacggggt tgtaaaaacg agaaattttt
     1381 acagccccat ttttatagct tttatcagtg cctttgcgac atatcgtcgt agatttcgca
     1441 aaaccgcgcg gcaaatgcgg gctcttcgtt ccctgcgtac agatgagaaa tatctccgaa
     1501 tgagctgcag cgccataccg ctatgccttt ttctcgctcc tcggtgtaca gtgtagtgac
     1561 cgaggtcgga gcagcgcaaa agccctgcca caggcatacg ggcgaaatgt tgagaagatg
     1621 tgaaagcatt acacattcca cgccgaaatg gcaaaagaac actattgtgt ctttgtttgc
     1681 atttacggcc ttgt
//
LOCUS       DBMDVO010000296         1694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34103_length_1694_cov_0.890772, whole genome shotgun sequence.
ACCESSION   DBMDVO010000296 DBMDVO010000000
VERSION     DBMDVO010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34103_length_1694_cov_0.890772"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(26..742)
                     /locus_tag="ACZPE2_04840"
     CDS             complement(26..742)
                     /locus_tag="ACZPE2_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319209.1"
                     /translation="MDKKIIAVICASLIVISVAFASCGKKKPTINAKDATYEVVTDEE
                     GNTVLNENGEIEIYVTDEDGKKVTDENGEIKTGAVPFPAQITDGVFHETKYFKMVYPN
                     GWTVGNSGIATSKKYKGLDAQVMYSGAATDDSAAENEANNTVSQWNQILSQAGDAVTG
                     VTVEQRTVTLEKCGATAYVVEVKKADDKNPKTYSAYQLTMDFVCNGGLYHVQCFTEDG
                     NTFDGSLDLAAMYGANLTIK"
     gene            complement(1273..>1694)
                     /locus_tag="ACZPE2_04845"
     CDS             complement(1273..>1694)
                     /locus_tag="ACZPE2_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HIR1319210.1"
                     /translation="DDELRDFAEKLSKIAADYNIKIGSCAEKIDLDECGIIHNCCIDR
                     ELIEKIIGCKLNVGKDKNQRKECGCVESVEIGTYNTCKNGCAYCYANYSSKSVETNAA
                     KYDPSSPLLCGQVQEDDKIAIRKVESLKETQLSIFDM"
BASE COUNT          434 a          393 c          308 g          559 t
ORIGIN      
        1 aatgcaatgt tctttatttg aactcttatt ttattgtgag atttgcgccg tacattgccg
       61 ccaaatcaag gcttccgtca aaggtgttgc cgtcctcggt aaaacactgc acgtggtaaa
      121 gcccgccgtt gcagacaaaa tccatggtga gctgatatgc ggaatatgtt ttcgggtttt
      181 tgtcgtccgc ctttttgacc tcgactacgt atgcggtggc tccgcatttt tcgagagtta
      241 cggtacgctg ctcaacggtt acgcccgtta ccgcgtcgcc tgcctgcgac aaaatctgat
      301 tccactgcga aacggtgtta tttgcctcat tttccgctgc gctgtcatca gtcgccgcgc
      361 cggaatacat aacctgcgcg tcgagcccct tgtatttctt tgaggtggct atacctgaat
      421 tgcccacggt ccagccgttg ggataaacca ttttaaaata ctttgtctcg tgaaatacgc
      481 cgtcggttat ctgagcgggg aacggaacgg cacctgtttt aatttcaccg ttttcgtccg
      541 tgaccttttt gccgtcctcg tcggtgacat atatttcaat ctcgccgttc tcattgagaa
      601 cagtgttgcc ctcttcgtcg gtaacaacct cgtatgtggc atccttggcg tttatagtcg
      661 gcttcttttt tccgcatgag gcaaaagcta ccgatattac gattaaggaa gcgcagataa
      721 ctgcgattat ttttttatcc atttgaaatc ctccgtttta tacataaaca gattataaat
      781 tctttgcctc ggaatgtcaa ccggggatgt taaaaatgtc cggaacgcaa aaaaaacaaa
      841 agacaaatct ttgtataaac aggtgttttt attcgtcaga gcttaccatt tgctccgaat
      901 caacctcacc ctcggagtac attcgtacgc cttcgggcaa gccttaaccg ccgcagccgc
      961 gccgcttaag gttcggttaa tccgctccga gcattttttc ctatcccccg aaaaaagagg
     1021 gtaaaaatgt ccggaacgca aaaaaacaaa agacaaatcg aaacaaatct ttgtataaac
     1081 agttgttttt attcgtcaga gcttaccatt tctcttgggc ggagagattt atccggccgt
     1141 ttcttggtac acatttgtgt attttattct tcatattcat ctcattttgc atttttgtgc
     1201 acctcgtcta aattttttag agccaaaatg gagccggata tactcttaaa aatatacgtt
     1261 ataataatga actcacatat caaaaatact tagctgtgtt tcttttaatg actcaacttt
     1321 tctaatcgct attttgtcat cttcttgaac ttgcccacat agaagaggtg acgaaggatc
     1381 atatttggca gcgtttgttt ctacactttt tgaactataa tttgcatagc aatatgcaca
     1441 tccatttttg catgtattat aagtacctat ttcaacgctt tcaacacatc cacattcttt
     1501 acgctgattt ttgtctttgc caacattgag tttacatccg ataatttttt caattagttc
     1561 tctatctatg caacaattat gaattatacc acattcatcc aaatcaattt tttctgcaca
     1621 acttccaatc ttaatattat aatccgcagc tatcttactt aatttctcag caaaatctcg
     1681 tagttcgtca tcat
//
LOCUS       DBMDVO010000297         1693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34123_length_1693_cov_1.230528, whole genome shotgun sequence.
ACCESSION   DBMDVO010000297 DBMDVO010000000
VERSION     DBMDVO010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34123_length_1693_cov_1.230528"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..1324)
                     /locus_tag="ACZPE2_04850"
     CDS             complement(8..1324)
                     /locus_tag="ACZPE2_04850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR1319211.1"
                     /translation="MQLYEKLVSGDEKLALVGLGYVGMPIAVAFSKRVKVVGFDLNAK
                     KIELYKNGIDPTNEVGNEAVKNCSVEFTADESKLKEARFIIVAVPTPVNDDHTPDLTP
                     VEGASEIVGRNLTKGSVVVYESTVYPGVTEDICVPILEKESGLKCGTDFKIGYSPERI
                     NPGDKVHRLETITKIVSGMDDETLDTVAKTYELVVKAGVHRAESIKVAEAAKVIENSQ
                     RDINIAFMNELSIIFNKMGIDTKSVLEAAGTKWNFLKFYPGLVGGHCIGVDPYYLTYK
                     AEEMGYHSQIILSGRRINDDMGKYVAENTVKNLIKADVPVKNAKVAILGFTFKENCPD
                     TRNTKIIDIYNELREYGITPVIADPEADADEAKRLYGIEFVDINDIKNCDAVILAVAH
                     EQFKSLTMNDFEAMFKHNENKNVLVDIKGLLDRKEYENAGYIYWRL"
     gene            complement(1311..>1693)
                     /locus_tag="ACZPE2_04855"
     CDS             complement(1311..>1693)
                     /locus_tag="ACZPE2_04855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1319212.1"
                     /translation="IIEHFGETDRVEDYYKQCSVYVLPSYREGTPRTVLEAMSMGRAI
                     ITTDAPGCRETVIDGKTGFLVPVKNAEAVAEKMTEFIEHPELIKTLGAASAEYAREKF
                     SVDKVNDDMCCHLNIERTNKYAVV"
BASE COUNT          383 a          432 c          346 g          532 t
ORIGIN      
        1 agctaaatta tagtctccag taaatatatc ctgcgttctc atattccttg cggtcaagca
       61 gtccttttat atcaacaagg acattcttgt tttcgttatg cttgaacatt gcttcaaagt
      121 cattcattgt taatgactta aactgctcat gagcaacggc aagtattacg gcgtcgcaat
      181 tttttatatc gttaatatcg acaaattcaa ttccgtaaag acgctttgct tcgtcagcat
      241 cagcttcagg gtcggctatt accggggtta ttccgtactc gcgaagctca ttgtatatgt
      301 cgatgatttt cgtatttctc gtgtcggggc aattttcttt aaacgtaaag ccgagaatcg
      361 caacctttgc gttttttacc gggacatccg cctttataag atttttaacg gtgttttcgg
      421 ctacatactt gcccatatcg tcgtttatgc gtctgccgga gaggataatc tggctgtgat
      481 agcccatttc ctcggccttg tatgtaaggt aatagggatc aacgcctatg cagtgtccgc
      541 cgacaagtcc gggatagaat ttgaggaaat tccactttgt gcctgccgcc tccaaaacgg
      601 attttgtatc tatgcccatt ttgttgaaga ttattgacag ctcattcata aatgcaatgt
      661 ttatatccct ctggctgttt tcaataacct ttgcagcctc ggcaaccttg atgctctccg
      721 ctctgtgtac gcctgccttt acgacaagct cataggtttt cgccacggta tcaagagttt
      781 catcgtccat accgcttact attttggtta ttgtctcaag gcggtgtacc ttgtcgccgg
      841 gatttattct ctcgggactg taaccgattt taaagtccgt accgcattta agccccgact
      901 ctttttcgag tatcggcacg caaatatcct cggtaacgcc gggataaacg gtgctctcat
      961 atacaactac cgagcctttt gtaagatttc tgccgacaat ttcgctcgct ccctctaccg
     1021 gtgtaaggtc gggggtgtgg tcgtcgttta cgggagtagg cacggcaaca attataaacc
     1081 ttgcctcctt gagcttgctt tcgtccgccg taaactcgac agagcaattc ttaacagcct
     1141 cgttgccgac ttcattggtc gggtctatgc cgttcttgta aagctctatt ttctttgcat
     1201 tgaggtcaaa gcctacaacc ttaaccctct ttgaaaacgc aacggcaatc ggcataccca
     1261 cgtatccgag gccgacaaga gccaattttt catcacccga aacgagcttt tcatacaact
     1321 gcatatttat tcgtcctttc tatgttaaga tgacagcaca tatcatcgtt aaccttatca
     1381 acgctgaatt tttcgcgggc atattccgcc gatgccgcac cgagcgtttt tatgagctcg
     1441 ggatgctcaa taaattccgt cattttttcg gcaaccgcct ctgcattttt tacgggaacc
     1501 aaaaatcccg ttttcccgtc aatcacggtt tcgcggcatc cgggtgcgtc ggtcgttata
     1561 atcgccctgc ccatgctcat cgcctccaag accgttctcg gagtgccctc gcggtatgac
     1621 ggtaaaacat ataccgagca ctgcttgtag taatcttcta ctctgtccgt ctcgccgaaa
     1681 tgctctatta ttc
//
LOCUS       DBMDVO010000298         1691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34186_length_1691_cov_0.750314, whole genome shotgun sequence.
ACCESSION   DBMDVO010000298 DBMDVO010000000
VERSION     DBMDVO010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34186_length_1691_cov_0.750314"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            171..1679
                     /locus_tag="ACZPE2_04860"
     CDS             171..1679
                     /locus_tag="ACZPE2_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR1319213.1"
                     /translation="MKDKAQKNIKMHSVQFNFIMNAILTVSSFIFPLITFPYISRTLL
                     VDGIGKVNFATSVISYFTMFASLGIPTYGIRACARVRDDREKLTKTAQELIIINLITS
                     AITYAVFFILLFTVDRFYQDKTLLTVTSVSIILNTLGVTWLYSALEQYSYITVRNIAC
                     KLVSVILMFIFVHNTSDYVIYGVIAVLASGGSNHLNFINLKKYISLRPVGHYDFKQHL
                     QPIFIFFATSIAISIYTNLDTVMLGFMTNDTQVGLYSASVKVKTVLLAVVSSLGNVLL
                     PRLSVYIHDNDYSKFREALSRVLNFLLLITIPLTLFFTLYVKDSIILLSGEAFLDASL
                     SMRLLMPTVFFCALSGMTGNQMLVPLGREKAVMLSVSIGAVFDFVLNFFVIPKMGAAG
                     AALSTLLTEFLVLVVQTFFLKDHLGKVIRGVKALPILISAAVSTGAGIVIQHYMGVSN
                     SFLRLVIGAVSFFGIYFVLLLILKEKFLTENLTLALEKLKSLLSSRNQQPKD"
BASE COUNT          439 a          372 c          344 g          536 t
ORIGIN      
        1 cctgccgaca caatacatac tcttgctttt taccgcgcaa tactgtataa tttattccaa
       61 aaataatgat aaatgacaca ttaattactc gctgcaaatg cagctgtttc cggtaaggcg
      121 tgttgtattg agcagtaatg atacaagcct tatcttatag gagtttttat atgaaggaca
      181 aagcacaaaa aaatattaag atgcattcgg ttcagttcaa cttcataatg aacgccatac
      241 tgaccgtttc ttcatttatt tttccgctga taacgttccc gtatatttcg cgtactcttt
      301 tggtagacgg tatcggaaag gtgaatttcg caacgtcggt aatttcatac tttaccatgt
      361 tcgcttcgct cggtataccg acatacggta tccgcgcgtg cgcaagagtt cgcgacgacc
      421 gcgaaaagct cacaaaaacc gcacaggaac ttataataat aaaccttatc acctcggcaa
      481 taacatacgc tgtatttttt atactgctct ttaccgttga caggttctat caggacaaaa
      541 cactgctcac cgtaaccagc gtttcgatca tactcaacac cttgggcgtt acatggcttt
      601 attcggctct tgagcaatat tcgtatatca cggtaaggaa cattgcctgc aagcttgtct
      661 ccgtaatatt gatgttcata ttcgtccaca acacatctga ttatgtgatt tacggtgtaa
      721 ttgcggtctt ggcatcgggc ggctcgaacc atttgaactt tatcaatctg aaaaaatata
      781 tttctctgcg acccgtaggg cattatgatt ttaagcagca tttgcagccg atattcattt
      841 tctttgcgac atcgattgct ataagcattt acacaaacct tgacacggtt atgctcggat
      901 ttatgaccaa cgacacacag gtaggcttat acagtgcctc cgttaaggtt aaaaccgttt
      961 tgctcgctgt ggtctcttca ctcggaaatg ttcttttacc gaggctgtcg gtatatatac
     1021 acgacaacga ttacagtaaa ttcagagagg cgctgtcaag agttctgaac ttcctgcttt
     1081 tgataactat acctttgacg ttattcttta cgctctacgt taaagactcc attatattgc
     1141 tttccggcga agccttctta gacgcctctc tttcaatgag acttttgatg cccacggtat
     1201 ttttctgcgc cctcagcgga atgacgggca atcagatgct ggtaccgctc ggcagagaaa
     1261 aggctgtaat gctgtcggta agcataggtg ctgtttttga ttttgtttta aacttcttcg
     1321 taattccgaa aatgggagct gccggtgcgg cactctcgac acttcttacg gaatttttgg
     1381 ttttggtggt acagacattc tttttaaagg accatctcgg caaagtcata cgcggagtaa
     1441 aggcattgcc cattcttata agtgctgccg tatccacggg tgccggaatc gtgatacagc
     1501 actatatggg tgtttcaaac tcatttttac ggcttgtcat cggtgcggtt tcgttctttg
     1561 gaatttattt tgttctgctt ctgattttaa aggaaaagtt ccttacggaa aaccttacgc
     1621 ttgcacttga aaagctgaaa tcgctgctgt cttcgagaaa tcagcagccg aaggattagt
     1681 tatttcacgg t
//
LOCUS       DBMDVO010000299         1688 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34241_length_1688_cov_1.367360, whole genome shotgun sequence.
ACCESSION   DBMDVO010000299 DBMDVO010000000
VERSION     DBMDVO010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34241_length_1688_cov_1.367360"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="ACZPE2_04865"
     CDS             complement(<1..764)
                     /locus_tag="ACZPE2_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319214.1"
                     /translation="MAKKQKVELTPEEKAAKKLRHSNGWVRFWAIVVALALTAGIYGL
                     ASKTAGKESAATGTATASGSSTGSSSSSSGSSSGSSSSSSSGASASSSSGASASAGDT
                     SGAAAAASSGVDAAAAAEAINAATAKAVAAGYHWTRSAQYTQPVDVGSATSGLNTLIK
                     GIDPEASLDSVVGGFIGIGDKEMDIEKGGSAAEQIDYHGENYALQATALQASDLKNLK
                     VDGNTYTFEVENATSPQTDKSTAMSRLTNDILTQSMV"
     gene            complement(835..>1688)
                     /locus_tag="ACZPE2_04870"
     CDS             complement(835..>1688)
                     /locus_tag="ACZPE2_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319215.1"
                     /translation="YISLNNKGVAAVSATPAQSGSQNAGGSASKSDWDTSANGSGSSQ
                     SGSQSSTSGDNASGGNAAAATGVDAAAAAEAINAATAKAVAAGYHWTRSAQYTQPIDV
                     GSATSGLNTLIKGIDSEASLDSVVGGFIGIGDKEMDIEKGGSAAEQIDYHGENYALQA
                     TSLKAEDLKNLKVDGNTYTFEVENATSPQTDKSTAMSRLTNDILTQSMVDSEIKNFIS
                     AAKINSATIEYTNIKATVVIEDGNLKEFKYSYDGNVAELNIKVSFISVSGKGAMHVDG
                     AYTNFVY"
BASE COUNT          352 a          434 c          432 g          470 t
ORIGIN      
        1 accatgctct gagtgagaat gtcgtttgta agtcttgaca tagcggtcga tttatccgtt
       61 tgaggtgaag ttgcgttttc aacctcaaag gtgtaggtgt ttccgtcaac cttaaggttt
      121 ttaaggtcgc ttgcctgaag agcggttgcc tgaagagcgt aattctcgcc gtgatagtca
      181 atctgctcgg ctgcgctgcc gcccttttcg atatccattt ccttgtcgcc gataccgatg
      241 aatccgccta caacggagtc aagagacgcc tcggggtcaa tacctttgat gagagtgtta
      301 agaccacttg tagcactacc gacgtcaaca ggctgagtgt actgagcgga tcttgtccag
      361 tgatagcctg ccgcaactgc cttagcggta gctgcattta tagcctcggc tgccgctgct
      421 gcgtctacgc ctgatgaagc tgccgctgcc gcaccgctgg tgtcaccggc agaagccgac
      481 gcgccgctcg aagaagaagc ggaagcgccc gatgaagagc tcgatgaaga acccgatgaa
      541 gagcccgacg aagagcttga tgagcctgtt gatgagcccg aagcagttgc cgtacccgtt
      601 gctgccgact ccttgcctgc ggttttgctt gcaagtccgt aaatgcctgc cgtaagagca
      661 agagccacta ctattgccca aaaacgtacc cagccgtttg aatgtctgag ctttttagct
      721 gccttttctt cgggggtaag ttcaaccttt tgttttttag ccattgattt ctctcccttt
      781 catttataaa gaagtgattt tatttttcat cattcggtaa atgccgaatt cttatcaata
      841 aacgaagttg gtgtaagcac catcaacgtg cattgcgccc ttaccgctta cgctgatgaa
      901 cgataccttg atattgagct cagctacgtt gccgtcatag gaatacttga attccttaag
      961 gttgccgtcc tcgataacta cggttgcctt gatgttggtg tattcaattg ttgcggagtt
     1021 tatctttgcc gcgcttatga aattcttgat ttcgctgtca accatgctct gagtgagaat
     1081 gtcgtttgta agtcttgaca tagcggtcga tttatccgtt tgaggtgaag ttgcgttttc
     1141 aacctcaaag gtgtaggtgt tgccgtcaac cttgagattc ttgaggtcct cggctttaag
     1201 cgaggtagcc tgaagagcat agttctcgcc gtgataatca atctgctcgg ctgcgctgcc
     1261 gcccttttcg atatccattt ccttgtcgcc gataccgatg aagccgccta caacggagtc
     1321 aagagacgcc tcggagtcaa tacctttgat gagagtgtta agaccacttg tagcactacc
     1381 gacatctata ggctgtgtgt actgagcgga tctcgtccag tgatagcctg ccgcaaccgc
     1441 ctttgcggta gctgcgttta tagcctcggc tgctgccgct gcgtccacgc ctgttgctgc
     1501 cgccgcattt cctccggaag cattgtcacc cgaggtgctg ctctgagaac ccgactgcga
     1561 agaaccgctg ccgtttgcgc ttgtatccca atcggatttg gacgcgctgc cgccggcatt
     1621 ctgcgagccg ctctgtgcgg gagttgccga aaccgctgcg acgcccttat tgttaagcga
     1681 aatatagg
//
LOCUS       DBMDVO010000300         1686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34291_length_1686_cov_0.984858, whole genome shotgun sequence.
ACCESSION   DBMDVO010000300 DBMDVO010000000
VERSION     DBMDVO010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34291_length_1686_cov_0.984858"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="ACZPE2_04875"
     CDS             complement(<1..327)
                     /locus_tag="ACZPE2_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1319216.1"
                     /translation="MQRKLVKCTINGKYTETVVDVRASLTDMLRNDYRLTSVKKGCEV
                     GECGACNVLIDGECFNSCIYLAIWADGKEIKTVEGLLGPNGELSDIQQAFIDEGAVQC
                     GFCTPGF"
     gene            complement(327..1208)
                     /gene="xdhB"
                     /locus_tag="ACZPE2_04880"
     CDS             complement(327..1208)
                     /gene="xdhB"
                     /locus_tag="ACZPE2_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002197 - xanthine dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit XdhB"
                     /protein_id="HIR1319217.1"
                     /translation="MYDIKALYEAESVENAVQLLTEHPEAVIIAGGSDVLIKMREGKL
                     AGCELVSIYGLDELRGVTMEEDGTIRIRPLTSFSHITKNPIIQKYINVLGEAVDMVGG
                     PQIRNIGTIGGNTCNGVTSADSASTLHAYDAIMEITGPDGVRTVPIHDFYISAGKVDL
                     KHGELLTSILIKKESYENYYGHYIKYAMRNAMDIATTGCSANVKLSDDKKTIVSARIA
                     YGVAAPVPIRAKSAEEFLNGKEATAENIAAFAKKSLDDVNPRTSWRASKELRLQLIEE
                     LSKRAMTEAVRLAGGEI"
     gene            complement(1224..>1686)
                     /locus_tag="ACZPE2_04885"
     CDS             complement(1224..>1686)
                     /locus_tag="ACZPE2_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR1319218.1"
                     /translation="SNAYSFGCCFAEIEVDIPLCKIKVLDIVNVHDCGKLINPQLAAA
                     QVHGGMSMAIGYALSEQLLYDPKTGKPLNNNLLDYKLSTIMDHPHLEAEFVENYEPTS
                     AYGTKALGEPPAVPAAPAIRNALLNATGVSVNELPLNPHKLFVRFKEEGLI"
BASE COUNT          420 a          424 c          326 g          516 t
ORIGIN      
        1 aaatcccggc gtgcagaagc cacactgaac agcaccttca tcaataaatg cctgctgaat
       61 atccgaaagc tctccgttag ggccgagaag tccttcgacc gtctttatct ccttgccgtc
      121 tgcccatatg gcgagatata tacatgaatt aaagcactct ccgtcaataa gcacgttgca
      181 ggcgccgcat tcgccgacct cacagccttt ttttaccgat gtaaggcggt aatcgtttct
      241 gagcatatct gtaagagaag ctcttacatc aacaaccgtt tcggtatact ttccgttaat
      301 tgtacattta acaagctttc tctgcatcag atttcacctc ctgcaagcct tacagcctct
      361 gtcatcgcac gctttgacag ctcctcgata agctggagtc tgagctcctt tgacgctctc
      421 cagcttgttc tgggatttac gtcatcaagt gatttttttg caaaagctgc tatattttca
      481 gcagtagctt cttttccatt caagaattcc tctgcacttt ttgcccttat gggaactgga
      541 gcggcaacgc cataggcaat tcttgcggaa actatcgttt ttttatcgtc cgaaagctta
      601 acattggctg aacagccggt tgttgcaatg tccattgcgt ttctcattgc atactttata
      661 taatgtccgt aataattttc gtagctttct tttttaatca atattgatgt aagcagttct
      721 ccatgcttaa gatcgacctt tccggcactt atataaaaat catgtatcgg aactgttctt
      781 acaccgtccg gacctgttat ttccataatg gcatcatagg catgaagtgt tgatgccgaa
      841 tcggcagatg taacgccgtt acaggtgttt ccgcctatcg taccgatatt tcttatctgt
      901 gggccgccga ccatgtcaac agcctctccg agaacattta tatatttctg tattatcgga
      961 ttttttgtta tatgcgaaaa gcttgtaagc ggacgaatac ggattgttcc gtcctcctcc
     1021 attgtaacgc ctctgagctc atcaagtccg taaatgctga caagctcaca gccggcgagc
     1081 tttccttctc tcatttttat aagaacgtcg cttcctccgg caattattac cgcctcggga
     1141 tgctccgtaa gaagctgaac cgcattttca acggattccg cttcgtataa agcctttata
     1201 tcatacatct gacagcacct cctttaaata agtccttctt ctttaaaacg cacaaacagc
     1261 ttatgcggat ttaaaggaag ctcatttacc gaaacacctg ttgcgttaag aagtgcattt
     1321 cttattgccg gagccgccgg aacagccgga ggctcgccga gtgcctttgt tccgtatgca
     1381 cttgtcggct catagttttc cacaaattca gcctcaagat gtggatgatc cattattgtg
     1441 gaaagcttat aatcaagaag gttattgttg agcggcttgc ctgtcttggg atcataaaga
     1501 agctgttcgc tcaaagcata tccgatagcc atactcattc cgccatgcac ctgtgccgcc
     1561 gcaagctgtg gattgataag ctttccgcag tcatgcacat tgacaatatc aagcaccttt
     1621 attttgcaaa gcggtatatc cacctctatc tccgcaaaac aacatccaaa tgaataagca
     1681 tttgat
//
LOCUS       DBMDVO010000301         1682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34393_length_1682_cov_1.309930, whole genome shotgun sequence.
ACCESSION   DBMDVO010000301 DBMDVO010000000
VERSION     DBMDVO010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34393_length_1682_cov_1.309930"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="ACZPE2_04890"
     CDS             complement(<1..1116)
                     /locus_tag="ACZPE2_04890"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1319219.1"
                     /translation="MSALVEMKGVVKKFPGVTALKDITFDIQPGEVHVLLGENGAGKS
                     TLMKILSGVYQPTEGEIIVGGKSYKRLTPKDSAENGISVIYQELSVINELSIQENLFV
                     GKLPMKKYGPVKVVDYKYMNERAKELLSKVGLKRDPKTLVEDISISEKQQVEIAKALA
                     ADSKVIIMDEPTASLTSEEVNHLFKIVRQLKEEGKGIVFISHKLGEIIEIGDRVSVLK
                     DGAYVGTRDVKGVTADDLVTMMVGRKIQNTYQHEDAPDTYENTEVIFEVRNLSRKDQK
                     VKNVSFKLHKGEILGFSGLVGSGRSEMMNAIFGSEPRSAGDIFIHGKQVKINSPYSAI
                     KNGLAMVTENRRETGFFYNFDIKQNISILPFIKTSKGK"
     gene            complement(1262..1657)
                     /locus_tag="ACZPE2_04895"
                     /pseudo
     CDS             complement(1262..1657)
                     /locus_tag="ACZPE2_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT          436 a          381 c          293 g          572 t
ORIGIN      
        1 ttttcccttt gaagtcttga taaacggaag aatcgaaata ttctgcttaa tatcaaagtt
       61 ataaaagaag cctgtctcac gacgattttc ggttaccatt gcaagaccat tctttatggc
      121 gctgtaagga ctattgattt taacctgttt gccgtgaatg aatatgtctc ctgccgagcg
      181 aggctcagac ccgaatattg cattcatcat ttcacttctg ccggaaccga caagtcccga
      241 gaatccgagg atttcaccct tatgaagttt aaacgagaca ttcttaacct tttgatcctt
      301 acgagacaaa ttacgaacct caaaaatgac ttctgtattc tcgtaggtat cgggagcatc
      361 ctcatgctga taagtattct gaatctttct tcctaccatc atggtaacga gatcgtctgc
      421 cgtaacacct tttacgtctc tcgtgccgac gtaagctccg tctttcagca ccgacaccct
      481 atcgcctatt tcaataattt caccgagctt gtgagaaata aagactattc ccttgccctc
      541 ttccttaagc tgacgaacta tcttaaacag atgatttact tcctcacttg tcaaagacgc
      601 agtaggctca tccattatga ttactttact gtcggcggca agtgccttag ctatttcaac
      661 ctgctgtttt tcggaaatac ttatatcctc aacaagagtt ttggggtctc gttttaatcc
      721 taccttcgag agcagctctt ttgcacgctc attcatatac ttataatcta cgaccttaac
      781 agggccgtat tttttcatcg gcaacttacc gacaaaaagg ttttcctgaa tggaaagttc
      841 atttatgacg ctcagttctt ggtaaattac gctgatgcca ttttcggctg aatccttggg
      901 agttagcctt ttatagcttt ttcctccgac aattatttct ccctcagtgg gctgataaac
      961 gccgctgaga attttcataa gtgtcgactt tccggcgccg ttttcaccga gcagtacatg
     1021 tacttcgccg ggctgtatgt caaatgttat atctttaagt gccgttacgc ccggaaactt
     1081 tttaacgacg cccttcatct caactaatgc gctcattttt tcctccttga agtctattat
     1141 ttccacatat tagttgtgtc cgtatgtgaa tagtatatac attttattgg gtaaaggttt
     1201 actcaatttc ccaaaatatg cgggaaataa tatttcccgt ttgcatatgt tagctgattt
     1261 tttatgcggt tgttctttta acaagcttta cgggaataac ataaatatca tttttatttt
     1321 ttgtctcatt attctcgatt aaatttaaaa gagtttcaac tgatttttta ccgaacatat
     1381 cggtatcctg ctgtattgtg gttatcggcg gatcgcatat ctctgacaga gtaatatcat
     1441 caaatccgac gaccttgcat ttttcaggca cagatattcc ggaatcctta agcgcctgca
     1501 aagcaccgat tgccatcata tcgttattac aaaaaattcc gtcaaacttt ttgccgtcag
     1561 ctatgacttt ttttatcgcg tcataagatg aagcgtaact gacatccgtt tttataacgt
     1621 ttccctcgtg aagcggcaaa gaatactttt tgtgagcgtc aacataacct tatatctgtt
     1681 tc
//
LOCUS       DBMDVO010000302         1682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34399_length_1682_cov_1.020873, whole genome shotgun sequence.
ACCESSION   DBMDVO010000302 DBMDVO010000000
VERSION     DBMDVO010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34399_length_1682_cov_1.020873"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="ACZPE2_04900"
     CDS             <1..192
                     /locus_tag="ACZPE2_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1319220.1"
                     /translation="SIMTDKLKEAILKLTDEEQLLIYRHYYADIPGTVLAEIYGVSQQ
                     AISKRIAKIRVKLKNLLEK"
     gene            661..1029
                     /locus_tag="ACZPE2_04905"
     CDS             661..1029
                     /locus_tag="ACZPE2_04905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1319221.1"
                     /translation="MKEIKRGEIYAVDFGTGCGSEQGGIRPSLILQNDIGNKHSPTTI
                     VAAITSRKTKATLPTHVKIMAKGLKTESTVLLEQIRTIDKARLGEYIDRLDSKTLAAV
                     DRAIVVSLGIKYLEELIHGR"
     gene            1019..1399
                     /locus_tag="ACZPE2_04910"
     CDS             1019..1399
                     /locus_tag="ACZPE2_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1319222.1"
                     /translation="MDDNRITAYTFTRMQPQATEADFLTAQREMDAYCKENGYRIVGS
                     AFTKEPSEKMASVMDNILTDMKNKNAELLVIPDISRLGRNTATVAGIVKAFADEGIDI
                     EITADGSRPTERFAPDAEPFIQEM"
     gene            1478..1561
                     /locus_tag="ACZPE2_04915"
     CDS             1478..1561
                     /locus_tag="ACZPE2_04915"
                     /inference="COORDINATES: protein motif:HMM:NF042617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319223.1"
                     /translation="MQLITEDEYERIAQISAKHYGADLIYV"
BASE COUNT          501 a          429 c          408 g          344 t
ORIGIN      
        1 agcattatga cggacaagct caaggaagcc atcctcaagc tgaccgatga ggaacagctt
       61 ctcatctacc gccattatta cgccgatatt ccaggtactg tgcttgcgga aatctacggc
      121 gtatctcagc aggcaatcag caagcggatc gccaaaatca gagtaaagct caaaaatctt
      181 ttagaaaaat aaaaatttcg gttgtaggcc ccctcacttt ttaccatgaa aagtgagggg
      241 gctttctcaa tagcctccgc ccgttctttg aaaaccgaat agcacagcgc attctttacc
      301 ttccttctgc cgtcggcgag agccgatgag ccacacgacc tatgagtcaa acggggcggc
      361 gcatccgcac cgaaggccat gacaagcaga agcaataatg atacttgtgc tcagtcacag
      421 gtcgagcgtt gaagcggcac gaaaaagcgc cgtgagccgt cgcaaccaat gagagcacct
      481 cgcagagatc ctgggagggg tgagatttcc atgaggcaaa atgcattgcc ggaggatgcg
      541 ttcccttgtt cggggtgtcg tggacaaata cggacaaaag caaaaacgat tgatacaaat
      601 accaaggcgg tcgtttgccc aaaagcactc gaccgccttt tacttaaaac ggaggctaat
      661 atgaaagaga ttaaacgagg tgaaatctat gccgtcgatt tcggcacagg ctgcggcagt
      721 gagcaaggcg gaattcgccc gtccttgatt ttacagaatg atatcggcaa caagcacagc
      781 cccaccacca tcgtggcggc gatcacaagc agaaaaacaa aggcaactct gcccacccat
      841 gtaaaaatca tggcgaaagg gcttaaaacc gaatccaccg ttctgctcga acaaatcagg
      901 acgatagaca aagcgaggct cggcgaatac atcgacaggc tcgacagcaa gacccttgcc
      961 gccgttgacc gtgccattgt ggtaagcctc ggcatcaaat atttggagga actgattcat
     1021 ggacgataac cgcatcaccg cctatacctt caccagaatg cagccgcagg cgacggaagc
     1081 agacttcctt acggcgcaaa gagaaatgga cgcctactgc aaggaaaacg gataccgcat
     1141 cgtcggctct gcttttacca aagaaccgtc ggagaaaatg gcatctgtta tggataacat
     1201 tctcaccgac atgaaaaaca agaacgccga gctgctcgtc atccccgaca tcagccgttt
     1261 gggaaggaac accgctacag tagccggcat cgtcaaagcc tttgcggatg agggcatcga
     1321 cattgaaata actgcagacg gctctcgccc gaccgaacgg tttgcaccgg acgccgaacc
     1381 gttcatccaa gaaatgtgag gtgcgaatat gaataagaaa gacatcggca actcctcttt
     1441 tgctttcagt gtaagcctat taaaaatgct cctcgccatg caactcatca ccgaggatga
     1501 atacgaaaga atcgctcaga tcagtgccaa acattacggt gctgatctta tttatgtctg
     1561 aaatcttaat tctcatgttt ttgcgaaaaa ccgctggatg tattcgtact cctgtggtat
     1621 tgtgtgttgc cgaagggcaa aaaaacagca aggaaaccct tgcggaaagg agaaagccat
     1681 ga
//
LOCUS       DBMDVO010000303         1681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34432_length_1681_cov_0.952532, whole genome shotgun sequence.
ACCESSION   DBMDVO010000303 DBMDVO010000000
VERSION     DBMDVO010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34432_length_1681_cov_0.952532"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1681
                     /locus_tag="ACZPE2_04920"
     CDS             <1..>1681
                     /locus_tag="ACZPE2_04920"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR1319224.1"
                     /translation="DISREFLNSNGAEKHTLVTVTNPQPIVKSVEGKTNGEKFLNLAD
                     DLNICSKRGLSERFDSTIGANTVLMPFGGKYQMTPAQGMAAKIPVLNKDTKTCSVMAW
                     GYNPFITEKNQYLGAYLAVVESVSKLIATGGNTENCYLTFQEYFERMTSDSKRWGQPT
                     AALLGALTAQLELGIASIGGKDSMSGTFEDIDVPPTLVSFAVSVGSSDDVMSPEWKLP
                     CSKVYYIEPKKNSDGTFDTESLKAVFARVEKLIKEKKALAVFTPSYGGIAEGVMKACF
                     GNKIGFAFDKRFPADKIYDYNYGSFVIEAACELDDAVLLGETTMDYSVKIGIEKIDLN
                     EIQKVYENKLEPVFACNIKTPEKAVNAYSFTAKTRISPKIGIKKPKVLIPVFPGTNCE
                     YDTTRAFINAGADPEVMVIKNLSASDVTESVNVFAEKIEASQIIFIPGGFSGGDEPDG
                     SGKFITAFFRNPVIKEKVHALLKERDGLMAGICNGFQALIKLGLVPYGEIIDPSADTP
                     TLTFNTIGRHQSRLVNTRVASNKSPWLSSSNVGDIHTVAISHGEGRFVCPK"
BASE COUNT          482 a          418 c          389 g          392 t
ORIGIN      
        1 tcgatatttc ccgtgaattc ttaaattcaa acggtgccga aaagcacact cttgtaaccg
       61 ttacaaatcc tcagcccata gttaaaagcg ttgagggtaa aaccaacggt gagaaattct
      121 tgaatcttgc cgatgacctt aatatttgct caaagcgcgg actctcggag cgttttgact
      181 caacaatagg cgcaaatacc gtgcttatgc ccttcggcgg aaaatatcag atgacgccgg
      241 ctcaaggcat ggcggcaaaa attcccgttc ttaataagga cacaaagacc tgctcggtta
      301 tggcttgggg ctacaaccca tttatcaccg agaaaaatca gtatctcggc gcataccttg
      361 cggttgtgga gtccgtatca aagcttatag ccacgggcgg aaacacggaa aactgctatc
      421 tcacattcca ggaatatttt gagagaatga caagcgacag caagcgttgg ggtcagccca
      481 ccgcggcact tctcggcgca cttacggctc agcttgagct cggtatagcg tcaataggcg
      541 gcaaggattc catgagcggc acattcgagg atattgacgt tcctcctact ctcgtttcat
      601 ttgcggtttc cgtcggttca agcgacgatg ttatgtcgcc cgagtggaag ctcccatgct
      661 ctaaggtcta ctacatagaa ccgaagaaaa attccgacgg cacatttgac acagagagcc
      721 ttaaagcggt atttgcaaga gttgaaaagc tcattaagga gaaaaaggct ctcgccgtat
      781 ttaccccgtc ttacggcggc attgccgagg gcgttatgaa ggcctgcttc ggaaacaaaa
      841 taggctttgc ttttgacaag agattcccgg cggacaagat atatgattac aactacggct
      901 cgtttgttat agaggccgcc tgtgagcttg acgacgcggt tctcctcggc gaaacaacca
      961 tggattattc cgtaaagata ggcattgaaa aaatcgatct caacgaaatc caaaaagtat
     1021 acgagaacaa gcttgaaccc gtatttgcct gcaacataaa gactcccgaa aaggctgtaa
     1081 acgcatacag ctttacggca aaaacgcgca tttcgcccaa aatcggcatc aaaaagccca
     1141 aggttttgat tcccgtattc cccggaacga actgcgaata cgatacaaca agggcgttta
     1201 ttaacgccgg cgcagatccc gaagttatgg taattaaaaa cctctctgca tctgacgtaa
     1261 ccgagagcgt aaatgttttc gccgaaaaaa ttgaagcttc gcagataata ttcattcccg
     1321 gcggcttctc cggcggcgac gagccggacg gcagcggtaa atttataacc gcattcttcc
     1381 gcaatcccgt cataaaagaa aaagtccacg cactgttaaa agaacgtgac ggacttatgg
     1441 ccggtatctg caacggcttc caggcactta taaaactcgg tcttgtcccc tacggtgaaa
     1501 tcatagatcc gagtgccgac acaccgacgc ttacctttaa caccataggc agacatcagt
     1561 caagacttgt caacacgaga gttgcctcaa acaagtctcc gtggctgtcg tcctcaaatg
     1621 taggcgatat acatactgtt gcgatttctc acggcgaagg caggtttgtt tgcccgaaag
     1681 a
//
LOCUS       DBMDVO010000304         1678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34493_length_1678_cov_1.746354, whole genome shotgun sequence.
ACCESSION   DBMDVO010000304 DBMDVO010000000
VERSION     DBMDVO010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34493_length_1678_cov_1.746354"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="ACZPE2_04925"
     CDS             <1..207
                     /locus_tag="ACZPE2_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319225.1"
                     /translation="YNPFADTLRIECEISRDSGSSYFDYGPTIAESQLIKDMIAEKLM
                     QGHGRTPQEFCEDVGGIEIGGMMQ"
     gene            204..464
                     /locus_tag="ACZPE2_04930"
     CDS             204..464
                     /locus_tag="ACZPE2_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319226.1"
                     /translation="MTQYIYPQNLKATANLWLWSLRDFVILAVAALLSVLALVQLHIF
                     LPAVAVLCYGFLTIRMDDTTVLDFMRYAVRYLISTQQYYEWR"
     gene            470..1063
                     /locus_tag="ACZPE2_04935"
     CDS             470..1063
                     /locus_tag="ACZPE2_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319227.1"
                     /translation="MKQQKKKKGRSVQELMGIKTFTKYGLSTNKGELLFYLVSPTNIS
                     VLSHTNIEIKIRHLMMVLSALPDIEIVCTDSSECFDDNKAYLTERLKTEENGRVKKLI
                     KKDIDFLDNIQTEMATARQFFFVARLKSQKEKQVFETANRIESKIAGEGFEVHRLKKP
                     EIKRILALYFEASMQGEAMPDIDGEQFYEGTDDEDEE"
     gene            1107..>1678
                     /locus_tag="ACZPE2_04940"
     CDS             1107..>1678
                     /locus_tag="ACZPE2_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1319228.1"
                     /translation="MLSGVKIHSELKERQKEKKDFASVAEIAKLPMTEAPAESVTDTK
                     TETSAEPTEQPVAGHNIQALIAENADCIGWLSIDGTSISYPVMYTPSEPQKYLRRSFY
                     GKYSQSGVPFLDGRCSTVGGNLIIYGHNMKNGTMFSDLKKYLNTDFLNSHRTVRLETA
                     DSVFLFTVTEVRRTNTADPWYDRTTCEDGR"
BASE COUNT          470 a          426 c          428 g          354 t
ORIGIN      
        1 tacaacccct ttgccgacac cctgcgtatc gaatgcgaga tcagccgcga cagtggctcc
       61 tcatactttg actatgggcc gacgattgca gaatcgcagc tcattaaaga catgatcgcg
      121 gaaaagctca tgcagggaca cggtcggacg ccgcaggagt tttgcgagga tgtcggcggc
      181 atagaaatcg gaggaatgat gcaatgacac aatatatcta tccgcagaac ttaaaagcca
      241 ccgccaatct gtggctttgg agtttgcggg actttgtaat acttgcggta gccgcactgt
      301 tgtcggttct cgcacttgtg cagctgcata ttttcttacc agccgtggct gttttatgct
      361 acggtttttt aacgatccgc atggacgata ccaccgtact cgacttcatg cgttacgccg
      421 tgcggtattt aatatccaca caacagtatt acgaatggag atgatgacaa tgaaacagca
      481 gaaaaagaaa aaaggccgtt cggtgcagga acttatgggt atcaagacct tcacgaaata
      541 cggcctctct acaaacaagg gcgagctgct cttttacctt gtgtcgccca ccaatatatc
      601 ggtgctctcc cacactaaca ttgagatcaa gatacgccat ctgatgatgg tgctgtcggc
      661 attgcccgac atcgagatcg tctgcaccga ctcctcggaa tgctttgacg acaacaaagc
      721 ctatttgacc gaacgcctca aaaccgagga aaacggcaga gtcaaaaagc tcatcaaaaa
      781 ggacatcgat ttcctcgaca acattcagac ggaaatggcg acggcgcggc agtttttctt
      841 tgtggcacgc ctgaaaagcc aaaaggaaaa gcaggttttt gaaaccgcca accgcatcga
      901 aagtaagatc gccggcgagg gctttgaggt acacaggctg aaaaaaccgg agatcaagcg
      961 cattctggcg ttatactttg aagcgagtat gcagggtgag gccatgcccg acattgacgg
     1021 cgaacagttt tacgagggta cggatgatga ggacgaggaa taagcccgcc ctcatatttt
     1081 tctgtcttct gctgtcggcg gcagccgtgc tgtcaggcgt gaaaatccat tccgaactga
     1141 aagaacggca gaaagaaaaa aaggacttcg cctccgtcgc agaaattgcc aaactgccga
     1201 tgaccgaagc tccggcagaa tccgtaacgg acacaaaaac agaaacgagc gcagagccga
     1261 ccgagcaacc tgtggcagga cacaatatac aggctctcat tgcggaaaac gccgactgca
     1321 tcgggtggct gagtattgac ggcacaagca tcagctatcc cgtcatgtat actccgagtg
     1381 aaccgcagaa atatctccgc cgcagcttct acggcaagta cagtcaaagc ggagtgccgt
     1441 ttcttgacgg acggtgcagc acggtcggcg gcaacctcat catttacggt cacaacatga
     1501 aaaacggcac gatgttctcc gatttgaaga aatatctgaa tacggatttt ctgaattcac
     1561 accgcacggt aaggctggaa accgccgaca gtgtgtttct gtttaccgtg accgaggtgc
     1621 ggagaaccaa taccgcagac ccatggtatg accgcacaac ctgcgaggat ggcagaca
//
LOCUS       DBMDVO010000305         1678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34499_length_1678_cov_1.420419, whole genome shotgun sequence.
ACCESSION   DBMDVO010000305 DBMDVO010000000
VERSION     DBMDVO010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34499_length_1678_cov_1.420419"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>524)
                     /locus_tag="ACZPE2_04945"
     CDS             complement(<1..>524)
                     /locus_tag="ACZPE2_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1319229.1"
                     /translation="EFEYKLAKKISDSVETVDMFRMMGSGTEACMGAARIARLATKHK
                     NIIKMGGAYHGWSDQMAYGIRVNGTKFTQAHGVPLNCFMFIQETTPNDLDDLEKKLRL
                     NQFRGGTAAVYMEPVGPESGTTPITVEYVKGVERLCRKYGALLVFDEVVTGFRIGMAG
                     AQGYFGVSPDLTIF"
     assembly_gap    525..534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<535..940)
                     /locus_tag="ACZPE2_04950"
     CDS             complement(<535..940)
                     /locus_tag="ACZPE2_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR1319230.1"
                     /translation="MDTRFAISEYNDAVAVNKELDELIKKPIYTIKPEALKKYEDEYY
                     NKKCLKSKAQIEEARQIIPGGVQHNLAFNYPFPLVFTKAEGAYLYDVDGNKYFDFLQA
                     GGPTVLGSNPKAVREQVIDLLNTCGPSTGLFHE"
BASE COUNT          452 a          374 c          417 g          425 t
ORIGIN      
        1 cgaatatggt aaggtcgggc gatacgccga agtaaccttg agcacctgcc ataccgatac
       61 ggaagcctgt aacaacctca tcgaatacga ggagtgcgcc gtatttgcgg caaagacgct
      121 caacgccttt aacatattca acggtgatgg gggtagtacc gctctcgggg cctaccggct
      181 ccatataaac ggctgctgtg ccgccgcgga actggttgag tctgagcttt ttctcaagat
      241 cgtcaagatc gttcggagta gtctcctgaa tgaacatgaa gcagttgagc ggaacaccgt
      301 gagcctgtgt gaacttggtg ccgtttacgc ggataccgta agccatctga tctgaccagc
      361 cgtggtaagc gccgcccatc tttatgatgt tcttatgctt tgtagcaagt ctcgcgatac
      421 gagccgcacc catgcaagcc tcggtgcccg agcccatcat acggaacata tcaacggttt
      481 caacgctgtc ggaaatcttt ttggcgagct tgtactcaaa ctcgnnnnnn nnnnactcgt
      541 ggaaaagtcc ggttgaggga ccgcaagtat taaggaggtc gatgacctgc tcacgaacag
      601 ctttcgggtt ggagccgaga acagtcgggc cgcctgcctg aaggaagtca aaatacttgt
      661 tgccgtcaac gtcatagaga tatgcaccct ctgcctttgt gaaaacaagc gggaagggat
      721 agttgaaagc aaggttatgc tgaacgccgc cggggattat ctggcgagct tcctcaatct
      781 gtgcttttga tttgaggcac tttttgttgt aatactcatc ctcgtatttt ttaagagcct
      841 ccggcttgat ggtgtaaatc ggctttttaa taagctcatc aagctctttg ttgactgcaa
      901 cagcgtcatt gtactccgaa attgcaaatc ttgtgtccat atcgtgacct ccgtttaata
      961 aaattaacga aacggtgctg cgcaaaagcg gcgcaccata gataaccacg tgtagtgaat
     1021 taccgttcac ttgtaattat agcacaaaaa agactcttgt caacgcaaaa atacgacgcg
     1081 gcaaacaaat cgaactcaaa attgcggacg tttagatgtt ttttaactat tatttgaatt
     1141 tgttaaacta aacagccgcg aatttgaaaa aatgacgaat acatctgtgg ggtgtaaatg
     1201 agcaactaca caatatattg atgattactc cgtcaaaata aataatcgat gtgttaatta
     1261 ttcgtcaatc tgccgaaatt tcgaaattgc ggcaaaaatc gcggattttt tgcataaatg
     1321 tctctttcga ctgccgaatt tgcattgcag ccgctttcag gctccatcaa atagggtgat
     1381 tccccacggt gtggggaaat gtctcgtagt gacaaaggga ctctctcccg tcaggagctg
     1441 gctgtgagca acgcgaacag actgagggat tattcaggct cccttgtaaa gggagctggc
     1501 tacgaacgta gtgagtagac tgagggattt aataggctcc cttgtaaggg gtgattcccc
     1561 acggtgtggg gaaatgtcgc ggagcgacaa aggggacggg ctcccgtcag gagctggctg
     1621 tgagcaacgt gaacagactg agggattaaa aaacaaaata ctacctattt aaaactca
//
LOCUS       DBMDVO010000306         1674 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34607_length_1674_cov_1.638907, whole genome shotgun sequence.
ACCESSION   DBMDVO010000306 DBMDVO010000000
VERSION     DBMDVO010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34607_length_1674_cov_1.638907"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="nifS"
                     /locus_tag="ACZPE2_04955"
     CDS             <1..1157
                     /gene="nifS"
                     /locus_tag="ACZPE2_04955"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR1319231.1"
                     /translation="KSALNAMAPYFCDIYGNPSSLHHMGQKAHSALEEARLATANAIG
                     CERSELYFTSGGSEADNWAIKGTAELMAKKGKKHIISTTVEHHAVLHTLKKLEKQGFE
                     ITLLPVSSDGFVSPGDVASAIKDDTALVTVMYANNEIGTIQPITEIGKICREKGVIFH
                     TDAVQAVGHLPIDVKAQNIDMLSMSGHKFHGPKGIGALYIRKGINLPNLIEGGAQERN
                     RRAGTENIPGIVGMAAALTEAVNNMEENTKKVAALRDRLIEKILKIPHSRLNGSRENR
                     LPGNVSVCFEGVEGESMLLYLDMQGVCASSGSACTSGSLDPSHVLLAIGLPHEVAHGS
                     LRLSLCEYNTEEEVDFIAEALPPIIERLRSMSPLWERLCRENSFFTHDKH"
     gene            1178..1606
                     /gene="nifU"
                     /locus_tag="ACZPE2_04960"
     CDS             1178..1606
                     /gene="nifU"
                     /locus_tag="ACZPE2_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR1319232.1"
                     /translation="MEYSAKVLEHFANPHNVGKIDDADGIGEVGNAKCGDIMKMYIKV
                     KDDVITDVKFNTYGCASAIATSSIATDMIKGKPISEALKLTNKAVVEALDGLPAVKIH
                     CSVLAEEAIKAAVKDYYDKNGIQYDEELFKPDNCHYTHEH"
BASE COUNT          529 a          396 c          401 g          348 t
ORIGIN      
        1 caaaatcggc tcttaatgca atggctccgt atttttgcga catttacggc aatccgtcga
       61 gtctgcacca tatgggtcaa aaagcacaca gcgctcttga ggaagcacgt ttagcgacag
      121 ctaacgcaat aggctgcgag agaagcgagc tgtatttcac atcgggcggc agcgaagcag
      181 acaattgggc cataaaaggc accgccgagc ttatggcaaa aaaaggtaaa aagcatatta
      241 taagcacaac cgtcgagcat cacgccgtac tgcacacatt aaaaaagctc gaaaagcaag
      301 gctttgaaat aactcttctc ccagtttcaa gcgacggctt tgtgtctccc ggcgacgttg
      361 cctcggcaat aaaagacgac acggctttgg taaccgtaat gtatgcaaac aacgaaatag
      421 gaacaattca gcccataacc gaaataggta aaatctgtcg cgaaaaggga gtcatcttcc
      481 acacggacgc agtacaggca gtcggtcatc ttccgataga cgtaaaggcg caaaacatag
      541 atatgctctc aatgtcgggt cacaagtttc acggaccgaa gggcatcggt gcgctttata
      601 taagaaaagg cataaatctg cctaacctta tcgagggcgg tgcacaagag agaaaccgca
      661 gagccggcac ggaaaacatc cccggaatcg tcggtatggc ggcagcactc acagaggccg
      721 taaacaatat ggaggaaaat acaaaaaagg tcgcagctct ccgcgacagg cttatagaaa
      781 aaatactcaa aattccgcac agccgcctga acggctcaag ggaaaatcgt ctgcccggaa
      841 atgtcagcgt ttgttttgaa ggcgttgagg gtgagtcaat gctgctctat ctcgatatgc
      901 agggtgtttg cgcgtcatca ggctccgcgt gcacatcggg ctcgcttgac ccaagccacg
      961 tgcttctcgc aataggattg cctcacgagg tcgctcacgg ttctttaaga ctgagcctgt
     1021 gcgaatacaa caccgaagaa gaggtagatt tcatcgccga agctctgcct cccataatag
     1081 aaagactgcg ttcaatgtct ccgctttggg agagactttg ccgcgaaaac agcttcttca
     1141 cccacgataa acattgagat aaggaaggat taaaaatatg gaatacagcg caaaagtcct
     1201 tgaacacttt gcaaatcctc ataacgtcgg aaaaatcgac gacgccgatg gcataggcga
     1261 ggtaggaaac gccaaatgcg gcgatataat gaaaatgtat ataaaggtta aagacgacgt
     1321 tataaccgac gtgaaattca acacctacgg ctgtgcctcc gcaatagcca caagctcgat
     1381 tgctacggat atgataaagg ggaagcccat aagcgaagca ttgaagctca caaacaaagc
     1441 cgttgtggaa gctcttgacg gactgccggc agtaaagatt cactgctcgg tactcgccga
     1501 ggaagctata aaggcggcag ttaaggatta ctatgacaag aacggcattc aatacgacga
     1561 ggaacttttt aagcccgata attgccacta tacacacgaa cactaaatca gacagaacca
     1621 aaaaggactg taaaacaatc gttttacagt ccttttattt tttgcggcat ttaa
//
LOCUS       DBMDVO010000307         1674 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34620_length_1674_cov_1.095995, whole genome shotgun sequence.
ACCESSION   DBMDVO010000307 DBMDVO010000000
VERSION     DBMDVO010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34620_length_1674_cov_1.095995"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>276)
                     /locus_tag="ACZPE2_04965"
     CDS             complement(<1..>276)
                     /locus_tag="ACZPE2_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1319233.1"
                     /translation="GSVVREYMPEELQKMSVDEINEAIRRDIYINAYEQQRKDPHKYK
                     GRALAEDLETILYLCPKCHAIGKTHSHDNEFSCECGYKMHINEYGFF"
     assembly_gap    277..286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<287..792)
                     /locus_tag="ACZPE2_04970"
     CDS             complement(<287..792)
                     /locus_tag="ACZPE2_04970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1319234.1"
                     /translation="MSSKKQRSKEMTTKTNNETPEALSHIKRAHGIYFTSKVLLGWII
                     KLYFNFRSKVYKPKNKSFILISNHTTMFDPFFEALSFKPYLRFVTSDHLLRMGAWGKF
                     IKFCVNPIPKRRGADSEKTMEMMAESVRNGVSVCIHAEGFCSINGETGFVSPRTGQLV
                     KDSGAGLIT"
     gene            complement(743..>1674)
                     /locus_tag="ACZPE2_04975"
     CDS             complement(743..>1674)
                     /locus_tag="ACZPE2_04975"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR1319235.1"
                     /translation="GTCILVPQFTVKTYAKMIKKNKPTVIPGVPTLFEALLRTEGLEG
                     VDLSFLQGVFCGGDSLSVELKKKVDAFLKEHNAKVQIREGYGTTECVTASCLTPRDFF
                     REGSIGIPYPDVYYQIVDPKDDSELPPDTEGEICISGPTVMKGYLNNAAETAATLRTH
                     ADGRVWLHTGDVGTMDNDGFIYFKQRLKRLIIVSGINVYPSQVENTIDAHPDVLLSCA
                     IGIPDPYKMHKVKAFVVLRPGVEPSEKIKEEILEHCRKNISKYAMPREIEFRTELPKT
                     LVGKVAYRKLEEEEAAKYEQQKAEKQGNDNENK"
BASE COUNT          376 a          401 c          403 g          484 t
ORIGIN      
        1 caaaaaatcc gtattcgttg atgtgcatct tatagccgca ttcacacgaa aactcattgt
       61 cgtgcgaatg tgttttacct atggcgtgac acttggggca aagataaaga atcgtttcga
      121 ggtcttctgc aagagcgcgt cccttatatt tatgcgggtc ctttctctgc tgttcatagg
      181 cgtttatgta tatgtcgcgg cgaattgctt cgtttatttc gtcaacggac attttttgaa
      241 gctcctcggg catatattcg cgaaccacgc tgccgcnnnn nnnnnngtga taaggccggc
      301 accgctgtcc ttgacaagct gaccggtgcg cggcgaaaca aagcccgttt caccgtttat
      361 cgagcagaac ccctcggcgt gtatacaaac gctgacgccg ttccttacgg attccgccat
      421 catttccatg gtcttttcgg agtcggcacc gcgtcttttc ggaatgggat taacgcagaa
      481 ctttatgaat tttccccagg cacccattct gagaagatgg tcgctcgtca caaagcgcag
      541 atacggttta aacgagagtg cttcaaaaaa cgggtcgaac attgtcgtat gatttgaaat
      601 gagaataaaa ctcttgtttt tcggcttgta taccttgctt ctgaaattga agtaaagttt
      661 gattatccag ccgagaagaa cctttgatgt gaagtagata ccgtgagcac gttttatgtg
      721 agataatgct tcgggcgttt cgttatttgt tttcgttgtc atttccttgc ttctctgctt
      781 tttgctgctc atattttgct gcctcctctt cttcaagctt cctgtacgct accttgccta
      841 caagagtctt gggaagctcc gtgcggaatt ctatttcgcg cggcatggcg tatttggata
      901 tatttttacg gcagtgctca agtatttctt cttttatctt ttcactcggt tcaacaccgg
      961 gacgaagaac gacaaaagcc ttgaccttgt gcatcttgta tgggtccgga atacctatgg
     1021 cgcatgagag cagtacatcc ggatgagcgt ctatggtgtt ttctacctgt gagggatata
     1081 cgtttatgcc ggaaactatg ataagacgct taagtctttg cttaaagtat atgaagccgt
     1141 cattatccat ggtgccgacg tcgcccgtat ggagccacac tctgccgtct gcatgggtgc
     1201 gaagcgtagc agccgtttcg gcggcattgt tgagatagcc cttcataacg gtgggaccgc
     1261 ttatgcagat ttcaccctct gtatcgggcg gaagctcgct gtcgtccttc ggatcgacta
     1321 tctgatagta aacgtccgga tacggtattc cgatactgcc ctcgcggaaa aagtcacgcg
     1381 gggtaaggca ggacgccgta acgcactccg ttgtaccgta gccctctcta atctgtacct
     1441 tagcgttgtg ctcctttaag aatgcgtcaa ccttcttttt aagctcaacc gaaagagagt
     1501 cgccgccgca gaaaacaccc tgtaagaacg agaggtcaac gccttcgagc ccctctgtgc
     1561 gaagcaaagc ctcaaagagc gtgggaacac cggggataac cgtaggtttg tttttcttta
     1621 tcatcttggc atatgtctta acggtgaact gcggaacgag tatgcacgtg cctc
//
LOCUS       DBMDVO010000308         1661 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_34986_length_1661_cov_1.526923, whole genome shotgun sequence.
ACCESSION   DBMDVO010000308 DBMDVO010000000
VERSION     DBMDVO010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34986_length_1661_cov_1.526923"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            61..>1661
                     /locus_tag="ACZPE2_04980"
     CDS             61..>1661
                     /locus_tag="ACZPE2_04980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1319236.1"
                     /translation="MSKGTILKVAGPLVVAEHMRDANMFDVVRVSDKRLIGEIIEMHG
                     DKASIQVYEDTSGLGTGEPVESTEEPLSVELGPGLIEGIFDGIQRPLVEIMKKVGNNL
                     PRGVEVPSLSREKKWHFNVTAEIGAYVTSGDELGFVQETDIVRHKIMVPIGVSGKVKS
                     LSEGDYTVEDTIGEIEKDDGTVVPVKLMQKWPVRRGRPYKKKLSPDVPLITGQRVIDA
                     LFPIAKGGVAAIPGPFGSGKTVTQHQLAKWAEADIVVYIGCGERGNEMTDVLNEFPEL
                     IDPHTGKSLMERTVLIANTSDMPVAAREASIYTGITIAEYYRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGRIITLGSDDKEGSLSVIGAVSP
                     PGGDISEPVSQATLRIVKVFWGLDSALAYKRHFPAINWLTSYSLYADSLGKWFNENVD
                     KDWTNMRTRIMGILSDEASLDEIVKLVGMDALSPSDRLKMEAARSIREDFLHQLAFHE
                     IDTYTSLKKQCFMMKLMLMYYDRSL"
BASE COUNT          437 a          409 c          449 g          366 t
ORIGIN      
        1 ggcgcagccg cagccgctaa actcgtaaaa tcaaccaaac ctttgaaggg tggaacagat
       61 atgagcaaag gaacaatact caaagtagcc ggacctctcg tagttgccga gcatatgcgt
      121 gacgcaaata tgttcgacgt tgtcagggta agcgacaaaa ggcttatcgg cgagataatc
      181 gagatgcacg gcgataaggc gtcaattcag gtatatgaag acacctcagg tctcggcacg
      241 ggcgagcctg ttgaatcaac ggaagaaccg ttgagcgttg agctcggccc gggacttatc
      301 gaaggaatat tcgacggtat tcaaagaccg cttgtcgaaa taatgaaaaa ggttggcaac
      361 aacttaccca gaggcgtaga ggtgccgtca ctctcgcgag agaaaaagtg gcattttaac
      421 gtcaccgccg aaatcggcgc atacgtgaca tcgggcgatg agctcggctt tgttcaggag
      481 accgatattg tccgccacaa aataatggta ccgataggcg tttccggtaa ggtgaagagc
      541 ctttcggagg gcgactacac ggttgaagat acgataggcg aaatagaaaa ggacgacggc
      601 accgttgttc ccgtaaagct tatgcagaag tggcccgtaa ggcgcggcag accgtacaag
      661 aaaaagcttt ctcccgacgt tccgcttatc acgggtcagc gcgttataga cgcgcttttc
      721 ccgatagcaa agggcggcgt tgccgcaata cccggtcctt tcggaagcgg caaaaccgta
      781 actcagcatc agctcgcaaa atgggcagag gctgatatag ttgtctatat aggctgcggg
      841 gaacgcggca atgagatgac cgacgtttta aacgaattcc ccgagcttat agatccgcac
      901 acaggcaaat cgcttatgga gcgcacggtt cttatagcga acacttcgga tatgcccgtt
      961 gcggctcgtg aagcttctat ttatacaggc attacaattg ccgaatatta tcgcgatatg
     1021 ggctattccg tggcacttat ggcggactcc acctcacgtt gggcagaggc tctgcgcgaa
     1081 atgtcgggca gacttgaaga aatgcccggt gaagagggct accccgcata cctcggttcg
     1141 cgactcgctc agttctatga gcgcgccgga cgtataatta ctctcggctc tgacgacaaa
     1201 gagggctcgc tttcggtaat aggtgccgta tcgcctcccg gcggcgacat ttcggagccg
     1261 gtatctcagg caactctgag aatagtaaag gtattctggg gtcttgattc cgcgctcgca
     1321 tataagcgtc acttcccggc tatcaactgg cttacgtctt attcgctcta tgccgacagc
     1381 ctcggaaaat ggttcaacga aaatgtcgac aaggattgga cgaatatgcg tacccgtata
     1441 atgggcattc tctccgacga agcgtcgctt gacgaaatag taaagcttgt cggtatggac
     1501 gccctgtcac cctccgacag acttaaaatg gaggcggcga ggagtattcg cgaggacttc
     1561 ctgcatcagc tcgcattcca cgaaatcgac acctacacaa gtcttaaaaa gcagtgcttt
     1621 atgatgaagc ttatgcttat gtattatgac cgctcgctcg a
//
LOCUS       DBMDVO010000309         1658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35089_length_1658_cov_1.148362, whole genome shotgun sequence.
ACCESSION   DBMDVO010000309 DBMDVO010000000
VERSION     DBMDVO010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35089_length_1658_cov_1.148362"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    303..312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<313..1440)
                     /locus_tag="ACZPE2_04985"
     CDS             complement(<313..1440)
                     /locus_tag="ACZPE2_04985"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HIR1319237.1"
                     /translation="MDKDINELRNEINSIDREFVELFIKRMGVSLSVAEYKKKTGMPV
                     LDRARERELLNRVAKMSGDEMKTYTRLLYSTVLNLSRSYQHSFLDNTSVLTEKILSAS
                     ANTADVFPTEAVVACQGIEGAYSSLACEKLFRYPDIMFFDSFDGVFSAVQNGLCRYGI
                     LPIENSTAGSVNKVYDLMSKNKFYIVRSIRLKVDHSLLAKKGTSLTDVKEIFSHEQAI
                     NQCSEFLNSLSGVKVTVCANTAMAAKMVAESNRRDVAAISSANCAGLYGLCQLSSDIQ
                     DTGNNYTRFICICKDLEVYPGADKTSLMMTLPHEPGALYNILARFFAYDINLLKLESR
                     PLKDRDFEFMFYFDIESPVHSDGFSRVITELSGSIDDFTYLG"
     gene            complement(1463..>1658)
                     /locus_tag="ACZPE2_04990"
     CDS             complement(1463..>1658)
                     /locus_tag="ACZPE2_04990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1319238.1"
                     /translation="GMPIVFRVAIKPTPSIGKPQRSVNLETMEEEELLIKGRHDPCIV
                     PRAVPAVEAVTAVTMLDIVL"
BASE COUNT          471 a          374 c          346 g          457 t
ORIGIN      
        1 gcttgcggaa ttgttaaata agcttttata aaaaaacaga taaaaaaaca aaccgtccgt
       61 tgggagatcg gacggtttgt tttttgcttt taagaagggg gagaaagtaa tgaaaaagtc
      121 taaaaagatc tgacaatctt tttatcaaat caacagctta tgtcgttctc ttgatttgat
      181 gtctttagta taaacacaaa atgtttcggc aaaaagtact tttgattaac attttgtaaa
      241 cagattattt agttttctta ataacttccc ccacgggatt tagtcacagc acttcggaat
      301 agnnnnnnnn nngccgagat acgtaaaatc gtcgatactt cccgacagct cggtaataac
      361 gcgagaaaag ccgtctgagt gaacgggcga ctctatatca aagtaaaaca taaattcaaa
      421 atccctgtcc tttaagggtc ggctttcgag ctttaaaagg tttatatcat acgcaaaaaa
      481 tctcgcaagt atattgtaaa gcgcacccgg ctcatgcggc agggtcatca taaggctcgt
      541 tttatcagca ccgggataga cttcaaggtc tttacagata catataaaac gagtgtaatt
      601 attgcccgta tcctgtatat ccgaggacag ctggcaaagc ccgtacagcc ctgcacagtt
      661 agccgaagat atagccgcaa catcacggcg attgctctct gcgaccattt tggcagccat
      721 tgccgtattg gcacaaacgg tgaccttaac tccgcttaga gaatttaaaa actccgagca
      781 ctgattgatt gcctgctcgt gagaaaaaat ctcttttaca tcggtaagcg acgttccttt
      841 ttttgcaagc agcgagtggt caaccttgag gcgtatactg cgtactatat aaaatttatt
      901 tttgctcata aggtcgtaaa ccttatttac ggagcctgcc gtgctgtttt cgataggcaa
      961 tatgccgtag cggcaaagcc cgttttgaac ggctgaaaag acgccgtcaa agctgtcaaa
     1021 aaacattata tcgggataac ggaacagctt ttcgcaggca aggctcgaat atgcgccctc
     1081 tataccctga cacgcgacaa cagcttcagt cgggaaaaca tcggcggtat ttgcggacgc
     1141 cgacagtatt ttttcggtca aaacagacgt attgtcaagg aagctgtgct gatacgaacg
     1201 ggagagattt aacacggtgg aataaagaag ccttgtgtat gttttcatct cgtcgccaga
     1261 catttttgcc acacggttta aaagctcccg ctcacgagca cggtcaagca ccggcatacc
     1321 ggtttttttc ttatactcgg ctaccgacag cgaaactccc atacgcttta taaaaagctc
     1381 cacaaattcg cggtcgatac tgtttatctc atttcgtaat tcattaatat ccttatccat
     1441 tgccattgcc ctttcggatt ttttaaagaa ctatatcaag cattgtcaca gccgttaccg
     1501 cttcgactgc cggtacggca cgcggcacta tacaagggtc gtgcctgccc tttatcaaaa
     1561 gctcctcttc ctccatagtt tcaaggttta cgctccgttg aggcttacct atggacggag
     1621 tcggctttat tgccacacgg aaaactatgg gcataccg
//
LOCUS       DBMDVO010000310         1648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35387_length_1648_cov_0.938591, whole genome shotgun sequence.
ACCESSION   DBMDVO010000310 DBMDVO010000000
VERSION     DBMDVO010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35387_length_1648_cov_0.938591"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="tyrS"
                     /locus_tag="ACZPE2_04995"
     CDS             complement(<1..619)
                     /gene="tyrS"
                     /locus_tag="ACZPE2_04995"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1319239.1"
                     /translation="MEIYEELKARGLIAQVTNEDEIRELVNAGKATFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPVVLIGGGTGYVGDPSGRTDMRSMMTPETIQHNCDCFK
                     KQMERFIEFGPDKAIMVNNADWLLKLNYIDLLRDVGACFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYHLFKHYGCNMQFGGDDQWSNMLGGTELIRK"
     misc_binding    complement(669..898)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(942..>1648)
                     /locus_tag="ACZPE2_05000"
     CDS             complement(942..>1648)
                     /locus_tag="ACZPE2_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1319240.1"
                     /translation="LAYLENAEKERDALSFSDERAEQLRAETEKAYNEALAAAKKLSE
                     IRIEAGKKFSADVERELAFLDMPSVKFIVNDSVGELYENGIDNIEFLLSANAGEEPKP
                     LSKIASGGELSRIMLAIKCVLSELDDIDTLIFDEIDSGVSGRAALKIAAKMKELSKTH
                     QVICVTHLAQIAAFADEHKLISKEEKDGRTYTCIASLDYNGRKYELARIMGGLTVTQS
                     ILNSAEELLSSAEIDD"
BASE COUNT          424 a          415 c          337 g          472 t
ORIGIN      
        1 tctttcttat aagctcagtt ccgccgagca tattgctcca ctgatcgtcg ccgccgaact
       61 gcatattgca gccgtaatgc ttaaacaaat gatagaagtc ataggactgc attatcatat
      121 agttaaactc aaggaacgaa agtccctttt ccattctctg cttatagcac tcggctctca
      181 gcatattatt gaccgagaaa caagcaccga catcacgtaa aaggtcgatg tagttgagct
      241 ttaagagcca atctgcgtta tttaccatta ttgccttgtc gggcccgaat tcaataaaac
      301 gctccatctg ctttttaaag caatcgcagt tatgctgtat ggtttcggga gtcatcatgg
      361 aacgcatatc ggttcttccg gacgggtcgc cgacatagcc tgtgccgccg cctatcaaaa
      421 cgaccggctt gttgcctgcc atctgaagac gtttcataag gcagagcgcc ataaagtgcc
      481 ctacgtgaag agagtccgcc gtcgggtcaa agcctatata gaaggttgct ttaccggcgt
      541 ttacaagctc gcgtatctca tcttcgttag ttacctgagc tattaagccc ctggctttta
      601 attcttcgta aatttccatt tcaattctct cctttaatta tcgacataaa aaagccctct
      661 gtcaaaagac agaggacgaa aaatcgcggt accacctcgg tttgcctttc cctcacggaa
      721 aaagcctcgt gaagttataa aaacttcggc aattaacgct tgcaaaacgg cataacctac
      781 tgtgcttcgg aaatgcggct ccgggacgta acttcaaaga gctttttacc gtttcgcagc
      841 aaccaacggc tctcttaaaa aaatttactc ttttaccttg acccttcgcg gcctgtataa
      901 atatgtgcct attgtaccat taaaaaaata tttgtcaacc tttaatcgtc aatttcggca
      961 gacgagagta attcctctgc gctgtttaaa atgctttgag taacggtgag gccgcccatt
     1021 atcctcgcaa gctcatactt tctgccgtta taatcgagag aagctatgca cgtgtaagtg
     1081 cgcccgtcct tctcttcttt tgaaataagc ttgtgctcgt cggcaaaggc tgctatctgc
     1141 gccaagtgag ttacgcatat aacctgatgt gtttttgaaa gctctttcat ctttgcggca
     1201 atctttaacg ccgctctgcc gctcacaccg gaatcgatct cgtcaaaaat gagggtgtcg
     1261 atgtcgtcaa gctcagacaa tacgcactta atggcaagca taattcttga aagctcgccg
     1321 cccgatgcta tttttgacaa aggcttcggt tcctcgccgg catttgccga gagcaaaaac
     1381 tcaatattgt ctatgccgtt ttcataaagc tcgccgacag aatcatttac aataaactta
     1441 accgacggca tatcgagaaa cgcaagctct ctttcgacat cggcggaaaa ctttttgccg
     1501 gcttcaattc tgatttcgga aagctttttt gccgcggcga gagcctcgtt atatgctttt
     1561 tcggtttccg cgcgcagctg ttcggctcgc tcgtccgaaa aagacaaagc gtctctctct
     1621 ttttcggcat tttcaagata agcgagta
//
LOCUS       DBMDVO010000311         1645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35461_length_1645_cov_1.332254, whole genome shotgun sequence.
ACCESSION   DBMDVO010000311 DBMDVO010000000
VERSION     DBMDVO010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35461_length_1645_cov_1.332254"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            168..251
                     /locus_tag="ACZPE2_05005"
     tRNA            168..251
                     /locus_tag="ACZPE2_05005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:gag)
     gene            complement(396..>1645)
                     /locus_tag="ACZPE2_05010"
     CDS             complement(396..>1645)
                     /locus_tag="ACZPE2_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1319241.1"
                     /translation="FHKFLEERFPLVYKNLKHEEISDASLLFTWEGTRPDLEPIALLA
                     HQDVVPIAAGTENDWEHPAFDGFDDGEFIWGRGALDMKNHLIAVIRTVETLLGEGFKP
                     ERTVYLCFGHNEEIVASENSGAGSIAAVLEERGVKLDSVIDEGGAVLNVDVPKILRTK
                     LAGIGIAEKGYADYKITVRSKGGHSSQPPVHSGIGEIAKVTRDLEGHQFKAKMPHFVY
                     ALFTKIGKRVSYPARIVTCNLWLLKPIVTLVMKKFPPAASLIRTTTGVSMAEGSPAAN
                     VLPQKSSITVNFRMMPGTTLADVKEHIKKVVKKKDIEIELLKGKEASKVSPTDSRSFK
                     ILEELCMRTDNNYIVAPYLVMGGTDSYHYENICDCIYRFAPFEVDTKYLLLTHATNER
                     IPVDCMEGALKFFKRYIKYMSKE"
BASE COUNT          408 a          387 c          389 g          461 t
ORIGIN      
        1 acgggtttaa agtgatattc agacttttcg gtactgtatt tatgggcctt aatttaccgc
       61 agtcgtaatt gtcaaaaaaa gtggtaactt tttttaaaaa ccgcttgaca caaaaggcaa
      121 cctttgctat aatattcacc gtcgcaagaa aagcgacaac atagtttgcg agcgtggcgg
      181 aatcggcaga cgcgcacgtt tgaggggcgt gtggtaatcc cgtatgggtt caagtcccat
      241 cgctcgcacc aaaaacgaca aatctctttt gggatttgtc gtttttattt ttgtgagttt
      301 atgcgacatc cgccgagagt aataatcgag gcgcaagcaa aagaccgcag agagcgtatt
      361 tgctttctgc ggtctttttg atttttaaga acagattatt cctttgacat atactttatg
      421 taacgcttaa agaatttaag tgcgccctcc atacagtcca cggggattct ctcatttgtg
      481 gcatgggtca aaaggagata ttttgtatcg acctcaaacg gggcgaaacg ataaatgcaa
      541 tcacaaatat tctcatagtg atatgagtct gtgccgccca taacaaggta cggtgcaact
      601 atatagttat tgtcggtgcg catacaaagc tcttcaagga ttttgaagga gcggctgtcc
      661 gtaggagaga cctttgacgc ctctttgccc ttgagcaatt cgatttctat atcctttttc
      721 tttacaacct ttttgatgtg ctcttttacg tccgcaagag tggttcccgg catcatacgg
      781 aagtttaccg ttatgctgct cttctgcgga agaacgttgg cggcagggct gccctctgcc
      841 attgaaacac ccgttgtggt tcttataagc gaagccgcag gcgggaattt tttcataaca
      901 agggtaacta taggctttaa gagccagaga ttgcacgtaa ctattcttgc aggatacgat
      961 acacgcttgc cgatttttgt aaagagcgca tacacaaagt gcggcatttt ggccttgaac
     1021 tgatgcccct caaggtcgcg tgtaaccttg gctatctcgc ctattcccga atgtaccgga
     1081 ggctgtgacg aatggccgcc ctttgagcga acggtgattt tataatcggc atatcccttt
     1141 tcggcaatgc ctatgccggc aagctttgtt ctgaggattt tcggcacatc cacattgagt
     1201 acggcaccgc cctcgtcgat aacgctgtca agctttactc cgcgttcttc gagaactgcg
     1261 gcaattgagc ccgcaccgga attttcggag gctactattt cctcattgtg gccgaagcag
     1321 agataaaccg ttctttcggg cttaaagcct tcaccgagaa gcgtctctac ggtccgaata
     1381 accgcgataa gatggttttt catatcgagc gcgccgcgtc cccatataaa ttcgccgtca
     1441 tcaaatccgt caaacgctgg atgctcccag tcattttcgg tgccggcagc tatcgggaca
     1501 acatcctgat gggcaagaag cgcgataggc tcaaggtcgg ggcgcgttcc ctcccatgtg
     1561 aacaaaaggc tcgcatcgct gatttcctcg tgcttcagat tcttgtaaac aagcgggaag
     1621 cgctcctcaa gaaatttatg aaatt
//
LOCUS       DBMDVO010000312         1644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35503_length_1644_cov_1.096565, whole genome shotgun sequence.
ACCESSION   DBMDVO010000312 DBMDVO010000000
VERSION     DBMDVO010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35503_length_1644_cov_1.096565"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="ACZPE2_05015"
     CDS             complement(<1..323)
                     /locus_tag="ACZPE2_05015"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR1319242.1"
                     /translation="MLKWFPCEMHCHTLHSDGKFTVESLMQTAKEYGLSGIALTDHNT
                     VSGWDEITEEREKKYVSVLKGIEWTTFFGHMLVTDCKEFVDWRDAVPDNIDEKIAEVK
                     KRGGMV"
     gene            complement(316..783)
                     /locus_tag="ACZPE2_05020"
     CDS             complement(316..783)
                     /locus_tag="ACZPE2_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319243.1"
                     /translation="MSLIFEETKTLTPNDTKTNVPLQFYVAEELEKMKIEFSYSPKNL
                     DDEEKAHKYIDDGFEKYAPEPYRKGYKPWYEYLPVKNLLTVSLDSPDGYIGCAHRQDS
                     RQTHIISETESSRGFIKTRLTSGLYRITINVHALVTDECTFDIKVFGEVKSNA"
     gene            complement(767..>1240)
                     /locus_tag="ACZPE2_05025"
     CDS             complement(767..>1240)
                     /locus_tag="ACZPE2_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-PLC domain-containing protein"
                     /protein_id="HIR1319244.1"
                     /translation="TENKIPKLGAVRGKIVLMRRCMLGTNDLSSVDNGLDFSYWEDQK
                     GTDSPLPLPCWFGNEKNEKAIIQDRYALKKEEKWQSAVTTMLGDYRPHKNIMALNFLS
                     TRGNPRENAEYINKMFSEYSLPDKPFGIIIFDFLTEALAEKVINTNTEGKDESDI"
     assembly_gap    1241..1250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1251..>1644)
                     /locus_tag="ACZPE2_05030"
     CDS             complement(<1251..>1644)
                     /locus_tag="ACZPE2_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012606.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PI-PLC domain-containing protein"
                     /protein_id="HIR1319245.1"
                     /translation="NIPDETPLSMISIPGTHDSAARFTTAPTPRIFCCQDMSVKNQLL
                     SGARFLDIRLILSHNKFKAVHFFIRCRKEKELSSGTLLFDDILCDTAEFLKENPTETV
                     IVSIKMDSGVNVKDFYKSFRSRYIDSQK"
BASE COUNT          357 a          385 c          365 g          527 t
ORIGIN      
        1 accataccgc cgcgcttttt gacctcggcg attttttcgt ctatgttgtc gggaacggcg
       61 tcgcgccagt ccacaaattc cttgcagtcg gtgacaagca tatggccgaa aaaggtagtc
      121 cattctattc ccttcagaac ggaaacgtat ttcttttctc tttcttcggt aatttcgtcc
      181 cagcccgaaa cggtgttgtg atctgtaagg gctatacccg agagcccgta ttccttagct
      241 gtttgcataa gcgactccac ggtgaatttg ccgtcgctgt gcagggtgtg acagtgcatt
      301 tcgcacggga accatttaag cattgctttt cacctcgccg aaaactttta tatcaaacgt
      361 acattcgtcg gtaacaagcg cgtgtacgtt tattgttatt ctgtaaaggc cgctcgtaag
      421 cctcgttttt ataaatccgc gagagctttc cgtttccgaa ataatgtgcg tttggcggct
      481 gtcctgcctg tgagcgcagc cgatatagcc gtccgggctg tcaagcgata ccgtgagcag
      541 atttttaacc ggcaggtatt cgtaccaagg cttatatccc tttctgtacg gctccggagc
      601 atacttttca aatccgtcgt ctatgtattt gtgcgccttt tcttcatcgt cgaggttttt
      661 cggcgaatac gaaaattcaa ttttcatttt ttcaagctcc tccgcaacat aaaattgcag
      721 cggaacgttg gtttttgtat cattgggtgt cagcgttttc gtctcttcaa atatcagact
      781 catcttttcc ctccgtattg gtgttaatga ccttttcggc aagagcctcg gtcaaaaagt
      841 caaatatgat tatgccgaac ggtttatcgg gcagagaata ttccgaaaac attttgttta
      901 tatattcggc attttctctc gggttgcctc ttgtgctcaa aaagtttaac gccataatat
      961 ttttgtgcgg tcggtaatcg ccgagcatgg ttgtgacggc agactgccat ttttcctctt
     1021 ttttaagggc gtatcggtcc tgtattattg ccttttcgtt tttttcgttc ccgaaccaac
     1081 acggcagcgg caggggactg tccgtccctt tctgatcctc ccaatacgaa aaatcaagtc
     1141 cgttgtcaac agaggagagg tcgtttgtgc cgagcataca acgcctcata agcactattt
     1201 tgccgcgaac cgctccgagc ttcggtatct tgttttccgt nnnnnnnnnn tctttttgcg
     1261 aatctatgta tcggcttctg aagcttttgt aaaaatcttt tacgttaacg ccggaatcca
     1321 tttttatgct cacgataacg gtttcggtcg ggttttcctt taaaaactcg gctgtgtcgc
     1381 aaagtatgtc gtcaaaaagc agcgttccgc ttgacagctc tttttctttg cggcatctta
     1441 taaaaaaatg taccgctttg aatttgttat gactcaatat aagccgtata tcgaggaatc
     1501 tcgctccgga caacagctga ttttttacgg acatatcctg acagcaaaat atccgaggcg
     1561 tcggagcggt tgtgaatctt gccgcgctgt cgtgagttcc gggaatactt atcattgaca
     1621 gcggtgtttc gtcgggaatg tttt
//
LOCUS       DBMDVO010000313         1643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35529_length_1643_cov_1.085603, whole genome shotgun sequence.
ACCESSION   DBMDVO010000313 DBMDVO010000000
VERSION     DBMDVO010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35529_length_1643_cov_1.085603"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ACZPE2_05035"
     CDS             complement(<1..449)
                     /locus_tag="ACZPE2_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015662.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319246.1"
                     /translation="MDNKKQIRRRILIFTVSVLFLFSLFAVDLFRIQIVNAADYSTQR
                     VALSETKSTIAASRGEILDCNGTPLVTNEQINSVVLNASYFPSTKEQDKRNEIILSLI
                     NLLESEGAAWNDNLPLVLNSDGSVSFKENADKDITYLKSKDALPAVLP"
     gene            complement(469..996)
                     /gene="mreD"
                     /locus_tag="ACZPE2_05040"
     CDS             complement(469..996)
                     /gene="mreD"
                     /locus_tag="ACZPE2_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1319247.1"
                     /translation="MRRSTKILFVRRTVFVLLILAVHILQNTRGLFPEIFGVKANPLI
                     PLVICIGMFEREIAGAVLGMLAGILWDSVSPMGDGYNALLLMLAGAAAGLLIDYLMRN
                     NLMTALLLSGFACLIYSVFYVVFFLLANGVDSTGYLFIRYYIPAAVYSFLFTPLWYII
                     VRAVMRRTKINVLEN"
     gene            complement(996..>1643)
                     /gene="mreC"
                     /locus_tag="ACZPE2_05045"
     CDS             complement(996..>1643)
                     /gene="mreC"
                     /locus_tag="ACZPE2_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1319248.1"
                     /translation="SFVSSSTYRDRISELESEVADYQSKLVDYEKLKKQVEAYEKFLE
                     VKEKNPDFKFVAGTVIGRDSADTFGSFVLNCGSNDGVKVNDPVISGEYLIGIVNEVTP
                     TSCVVLSVTDPKVNAAAYEIRTGEMGYTETTSKLALNGLLKLTGLTKDTSVAVGGIVC
                     TSGVGGIYPRGLIIGTVTAVSKEEGNISYYAEVKPEVDPGDVQDAFVITEFEGKN"
BASE COUNT          420 a          406 c          390 g          427 t
ORIGIN      
        1 ggcaaaaccg cgggcagagc gtccttggat tttaaatatg ttatgtcctt gtcggcattt
       61 tccttaaaag aaacagagcc gtccgaattt aacacgagcg gcagattgtc gttccaagcg
      121 gcaccctcgc tctcaagcag gtttataagc gacaggatta tttcgtttcg cttgtcctgc
      181 tcctttgtgg acgggaaata ggacgcgttc agcacgacgg aatttatctg ctcgtttgtg
      241 acaagcggcg ttccgttgca gtcgagtatt tcaccgcgcg atgcggctat tgtggatttt
      301 gtctcggaca gagcgacacg ctgcgtcgag tagtccgccg cattgacaat ttgaatacgg
      361 aacaaatcca ccgcgaaaag tgaaaataaa aacaggacgg atacggtaaa aataagtatt
      421 cttcgcctta tttgcttttt gttatccaaa tttgccgctc ctttctcatc agttttcaag
      481 aacgttgatt tttgtccgcc gcataacggc acgaactata atgtaccaca gcggcgtgaa
      541 aaggaatgaa tacaccgccg caggtatata gtagcgtata aacaggtagc ccgtgctgtc
      601 aactccgttt gcgagcagga aaaacacaac atagaatacc gagtatataa ggcatgcaaa
      661 gcccgacagc agcagcgccg tcataagatt gtttctcata aggtagtcta tgagcagacc
      721 tgctgccgca cccgcaagca taagcaaaag tgcattgtag ccgtcgccca tgggtgaaac
      781 gctgtcccaa agtataccgg cgagcatacc caaaacagct ccggctattt cacgctcaaa
      841 catacctatg caaatgacaa gcggaattaa aggattggct ttaacgccga atatttcggg
      901 gaaaagcccc cttgtgtttt gcaaaatgtg aaccgcaagt atcaaaagca caaatacggt
      961 tcttctgaca aacagtattt ttgttgacct acgcatcagt ttttaccctc gaattcggtt
     1021 atgacaaaag cgtcctgaac atctccggga tcaacctcgg gttttacctc ggcataatat
     1081 gagatgttgc cctcctcctt gcttacggcg gtcaccgtgc cgataatgag gccgcgcgga
     1141 taaattccgc cgacgcccga tgtgcaaact attccgccta ccgcaacgga ggtgtccttt
     1201 gtaagcccgg tgagcttcaa aaggccgttt agagcgagct ttgacgtggt ttctgtgtag
     1261 cccatttcac ccgtgcgtat ttcgtatgcc gccgcgttga ccttggggtc cgttacggag
     1321 agaacaacgc acgaggtcgg cgttacttcg tttactatgc ctattaagta ctcgccgctt
     1381 attacggggt cgttgacttt aactccgtcg tttgaaccgc agttcaaaac aaacgagccg
     1441 aaggtgtcgg cggagtctct gccgataacg gttccggcaa caaatttaaa atcgggattt
     1501 ttttctttga cctcaaggaa tttttcgtat gcctcgactt gctttttcag cttttcgtaa
     1561 tcgacaagct tgctttgata atcggcaacc tccgattcaa gctcgctgat acggtcgcga
     1621 taggtgctcg acgagacgaa gct
//
LOCUS       DBMDVO010000314         1640 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35616_length_1640_cov_1.235218, whole genome shotgun sequence.
ACCESSION   DBMDVO010000314 DBMDVO010000000
VERSION     DBMDVO010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35616_length_1640_cov_1.235218"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="ACZPE2_05050"
     CDS             <1..420
                     /locus_tag="ACZPE2_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR1319249.1"
                     /translation="PSGLKTGLAIFPAITAMMLAVNMFTKSGLCDYFTKLITPVFTNI
                     GIPAELIPLITLTPLSGSGSLSIFENLLKDYGCDSFIGRCASAVMASTETTFYVITVY
                     YGAIGIKKIRHTAFVSLCANMVSVITATLMVKLITFQ"
     gene            complement(407..>1640)
                     /gene="gpmI"
                     /locus_tag="ACZPE2_05055"
     CDS             complement(407..>1640)
                     /gene="gpmI"
                     /locus_tag="ACZPE2_05055"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1319250.1"
                     /translation="ENCKKYGTALHLIGLLSDGGVHSHNTHLYALLELAKRNGIKDVY
                     VHCLLDGRDVPPASGVEYIKELEDKMSEIGVGKIATLMGRFYAMDRDNMWDRVGMAYR
                     AMVNGEGVHNSNAVDAVEKSYESIDGDGKHLTDEFVMPTVCSEDGKIKENDSVVFFNF
                     RPDRAREITRAFVDPEFKGFERKYFPLYYVCMTQYDETMPNVKVAFAPEALKNTMGEY
                     LSSLGKTQLRIAETQKYAHVTFFFNGGEEKVYPGEDRVLIDSPKISTFDLKPEMSAYE
                     VCDAACERILSGKYDVVILNYANCDMVGHTGVFPAAVKAVETVDECVGKLVDAVNKMG
                     GVTLITADHGNADKMYAEDGTPFTAHTTNPVPLIVVGYDCKLKQNGGRLCDLAPTMLD
                     IMGLAKPAEMTGVSLIEK"
BASE COUNT          452 a          384 c          330 g          474 t
ORIGIN      
        1 ccgtcaggac tcaaaaccgg attggccatc ttcccggcga ttacggcaat gatgctggca
       61 gtaaatatgt ttacaaaatc gggtctctgc gattacttca caaaacttat aacgcctgtt
      121 tttacaaata tcggtattcc tgccgaactg atacctctga taacactaac tccgctgtca
      181 ggcagcgggt ccctaagcat atttgaaaat ctgctcaaag actatggctg cgactcattt
      241 ataggcagat gtgcttccgc tgtaatggcg tcaactgaaa caacttttta tgtaataacc
      301 gtttattacg gagcaatagg tataaaaaag ataaggcaca cagcttttgt ttcgctgtgt
      361 gccaatatgg ttagtgtaat tacagcaacc ctaatggtga agcttattac ttttcaataa
      421 gagaaacacc tgtcatctca gcaggttttg caagtcccat aatgtcaagc attgtaggcg
      481 caaggtcgca gagtctgccg ccattttgtt tgagcttgca atcataacct actacaatca
      541 acggaacggg attggttgtg tgggcagtaa acggtgttcc gtcctcagca tacattttat
      601 ctgcgttacc gtggtccgcg gttataagtg taacgccgcc catcttattg actgcatcaa
      661 caagcttacc gacgcattca tcaacagttt caacagcttt tactgccgca gggaaaacac
      721 ccgtgtgacc caccatatca cagttagcgt agttaagaat tacaacatcg tattttccgc
      781 tgagtattct ctcacaagcg gcgtcgcaga cctcataagc actcatctca ggcttcaagt
      841 caaatgttga aattttgggt gagtcgataa ggactctgtc ttcgcccgga tatacttttt
      901 cttctccgcc gttaaagaaa aatgtaacgt gcgcatattt ttgtgtctca gctatacgaa
      961 gctgtgtttt gccgagagaa gaaagatatt ctcccatagt atttttaaga gcctcaggtg
     1021 caaaagcgac cttaacattc ggcatagtct catcatactg agtcatgcaa acataataaa
     1081 gagggaaata cttcctttcg aatcccttaa attcaggatc cacaaaagct cttgttattt
     1141 ctcttgcacg gtccggacgg aaattaaaga ataccacaga atcattttct ttaattttgc
     1201 cgtcttcgga gcatactgtg ggcataacaa attcatctgt aagatgctta ccgtcaccat
     1261 cgattgactc ataagacttt tcgactgcat caacggcgtt tgaattgtgt acgccttcac
     1321 cgttgaccat agccctgtat gccattccga ctctgtccca catattgtca cggtccatag
     1381 cataaaatct gcccattaag gtagcaattt tgccaacacc gatttcgctc attttatctt
     1441 caagctcttt tatatactca acgccggatg ccggcggtac gtcacgaccg tcaagaagac
     1501 aatgaacata aacgtcctta atgccgttgc gtttagcgag ttccagtaag gcataaagat
     1561 gagtattgtg gctgtgtacg ccgccgtcgg ataaaaggcc tatcaagtgg agagccgtac
     1621 cgtatttttt gcaattttca
//
LOCUS       DBMDVO010000315         1639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35643_length_1639_cov_1.360858, whole genome shotgun sequence.
ACCESSION   DBMDVO010000315 DBMDVO010000000
VERSION     DBMDVO010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35643_length_1639_cov_1.360858"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="acpS"
                     /locus_tag="ACZPE2_05060"
     CDS             <1..357
                     /gene="acpS"
                     /locus_tag="ACZPE2_05060"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR1319251.1"
                     /translation="VKISRLEKALETPGFYEKVFGENERAEFEKRGARLESLAAAFAA
                     KEAFGKALGTGIRGFSLCEAEVLHKENGAPYLFLSGKAKAVSDKIHAEFSLSLSHDGD
                     YATAFVICVTKGENIL"
     gene            354..>1639
                     /locus_tag="ACZPE2_05065"
     CDS             354..>1639
                     /locus_tag="ACZPE2_05065"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1319252.1"
                     /translation="MMKILPSNEIREIEAKANQSGISYLRLMENAGSACAKAIRKKFD
                     KTTRRNVTIVCGKGKNGGDGYVIARKLAENEYNVNVVLTSREPPVGDAGEMLSRIRGT
                     DIGIYLYDDDTELCEELIVGADITVDAVFGTGFKGAADEKNARIFKAISKSLGYTVSI
                     DIPSGIEADSGYVKGAAVKAQMTIAVMALKNSLVTFPAAEFAGNVIVVPIGIPEEIFS
                     EYTGAYTLSPDEIRSKFPHRKENANKGDFGKGLIIAGSYDMPGAAVIASAAAVNSGAG
                     LIKLAFPDKAYPAVTSSCPEKILLPLMTNNNGRISSQNIKKIEDELGKCDAVLIGCGM
                     GCDHDTATIAEAVLKSSAVPVIIDADGINALKDNINVIKSCLSPVVITPHPGEAARIL
                     GCTVGEIEKDRKAACKAIFEKTGAVVVLKGSRTIVTA"
BASE COUNT          497 a          383 c          432 g          327 t
ORIGIN      
        1 gtaaaaatat caaggcttga aaaggcgttg gaaacgcccg gcttctatga aaaggttttc
       61 ggcgaaaacg agcgtgcgga atttgaaaaa cgcggcgcaa ggcttgaaag tctcgccgcc
      121 gcatttgcgg caaaggaagc attcggcaaa gccctgggaa ccggaatacg cggcttttcc
      181 ctgtgcgaag cggaagttct gcataaggaa aacggcgcac cgtacctttt tctgtccgga
      241 aaagcaaaag ccgtatcgga taaaatacac gccgaattct cgctgtcgct ctcgcacgac
      301 ggcgactatg ccacggcatt tgtaatctgc gttacgaaag gtgaaaacat attatgatga
      361 aaatattgcc gtcaaatgaa atcagagaga tagaggcgaa ggcaaaccaa agcggtatat
      421 cgtatttaag acttatggaa aacgccggca gcgcctgcgc caaggcgatc cgcaagaaat
      481 tcgacaagac gacaagacga aacgtaacca tcgtgtgcgg caagggcaaa aacggaggag
      541 acggatatgt tatagcgcga aagcttgccg aaaatgaata taatgtcaac gttgtactta
      601 catcacgtga gccgcccgtc ggagacgcag gcgagatgct gtcgcgaatt cgcggcacgg
      661 atataggcat ctatctttac gacgatgaca ccgagctttg tgaagagctc atagtcggag
      721 ccgacattac tgttgacgct gttttcggaa ccggatttaa aggagccgcc gacgaaaaga
      781 acgcgcgtat tttcaaggct atatcaaaaa gcctcggata caccgtaagc attgacatac
      841 cgagtggcat cgaggccgat tcgggatatg taaaaggtgc tgccgttaag gctcaaatga
      901 caatagcggt tatggcgctg aaaaattcac tcgtgacatt cccggctgcg gaattcgcag
      961 gcaatgttat agtggtaccg ataggaatcc ctgaggaaat attcagcgaa tacacgggag
     1021 cttataccct gagtcccgat gaaatacgtt ctaaatttcc gcacagaaaa gaaaacgcaa
     1081 acaagggcga cttcggcaaa ggccttatta ttgccggaag ctatgatatg ccgggagccg
     1141 cggtcatagc gtccgcagca gccgtaaaca gcggagcggg gcttataaag ctcgcctttc
     1201 cggacaaagc atatcccgcg gtaacctcat catgtccgga aaaaatactg ttgccgctta
     1261 tgacaaacaa taacggcaga atttcatcgc aaaacattaa aaagatagag gacgagctcg
     1321 gcaaatgtga cgccgtttta atagggtgcg gaatgggttg tgaccacgac accgccacca
     1381 tagcggaagc cgtgctgaaa agctccgccg tgcccgtaat aatagacgca gacggcataa
     1441 atgcccttaa agacaatata aatgttataa agagctgttt gtctccggta gtcataactc
     1501 ctcacccggg cgaagccgca agaattctcg gctgcacggt cggcgaaatc gaaaaagaca
     1561 gaaaagccgc ctgcaaggcc atattcgaaa agacaggcgc agtggttgtg ctcaaaggct
     1621 cgcgcacaat tgtgaccgc
//
LOCUS       DBMDVO010000316         1638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35668_length_1638_cov_1.559532, whole genome shotgun sequence.
ACCESSION   DBMDVO010000316 DBMDVO010000000
VERSION     DBMDVO010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35668_length_1638_cov_1.559532"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..>1638)
                     /locus_tag="ACZPE2_05070"
     CDS             complement(63..>1638)
                     /locus_tag="ACZPE2_05070"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1319253.1"
                     /translation="LFFLLCLSFGVLFVKMAELCGGNVSDVAASKNTVGVTVASSRGN
                     IYDCKMRPLVNCDTVLKAAIKPCEESLSSIRVIVPESEIPSVYSTLSQGKIAVCASNA
                     VFDEKNIKTAKTVVRYGENSLAPHITGYIDGDGNGVSGIEKCYNDILLSYSGTLKARC
                     GAAASGKLLEGAEITFLKDGYMSAGGVELTVDRDIQKLCESALGEFGIDSGAVVVLDS
                     ATSEIRAMASTPSFDRNAPEKSLNDSSSPFLNRAITPYSVGSVFKAVVACAALENGIP
                     TSYSIVCNGKYDLNGEISFGCFKKTGHGNMNMYSAMAESCNPYFISLALKTGKENICA
                     MGENLGLGQKIELADGWETKSGIMPSADSLISEADLCNLAFGQGKLLASPLQMSAVYA
                     AVANNGVYRAPSLMKAVTDENGKEIKRAELPVPRRVMSVDTAKTVTELLRETVVSGSG
                     KRAETEYFDAAGKTATAQSGWFDENGNEVTHSWFCGFFPYSSPRYVITVFKENGAGGA
                     LDCAPVFKYIADGIKR"
BASE COUNT          410 a          455 c          356 g          417 t
ORIGIN      
        1 ttatgggaaa ataataacat tacggaacag aaaataaagc ttttgtgccg aaacagcaca
       61 agttatcttt taattccgtc ggcaatgtat ttaaacaccg gagcgcagtc taaggcaccg
      121 cccgctccgt tttctttaaa gactgtgatg acatagcgcg gcgaagaata cggaaaaaat
      181 ccgcaaaacc acgagtgcgt cacttcgttc ccgttctcgt caaaccagcc gctttgcgcc
      241 gttgccgttt tgcctgcggc atcaaaatac tcggtttccg ccctttttcc gctgccggac
      301 acaacggttt cgcgtaaaag ctcggtaact gtttttgccg tatcaacgct cattacacgc
      361 ctcggaacgg gcagctccgc ccttttgatt tcctttccgt tctcatccgt tacggctttc
      421 ataagagagg gcgcgcgata cactccgtta tttgccaccg ccgcatacac ggcggacatt
      481 tgcagcggcg aagcaagcaa tttgccctga ccgaatgcga ggttgcacaa atccgcctca
      541 gaaatcaagc tgtcggcgga cggcataata ccggactttg tctcccaacc gtccgcaagt
      601 tcgatttttt gaccgagccc gagattttca cccatggcgc aaatgttttc tttgccggtt
      661 ttaagcgcaa gacttataaa atacggattg catgactccg ccatagccga atacatattc
      721 atattgccgt gccccgtctt tttaaaacag ccgaacgata tttctccgtt caagtcgtac
      781 ttaccgttgc agacgatgct gtaacttgta ggaattccgt tttccaaggc ggcacaggcg
      841 acaaccgctt taaaaaccga gcctactgag tacggagtta tcgctctgtt aaggaacgga
      901 gaagatgagt cgtttaggct tttttcgggc gcatttcggt caaacgacgg tgtgctcgcc
      961 attgccctta tttcggaggt ggcgctgtcg agaacaacga ccgcaccgct gtcgataccg
     1021 aattctccga gagccgattc acacagcttt tgtatatctc tgtcaactgt gagctccact
     1081 ccgccggccg acatataccc atcctttaaa aatgtaatct ccgcaccctc aagcaatttt
     1141 cccgaagccg ccgcgccaca ccttgccttc agagttcccg aatacgacaa aagtatatcg
     1201 ttgtagcact tttcaattcc cgaaacgccg tttccgtcgc cgtcgatata tccggttatg
     1261 tgcggcgcaa ggctgttttc accgtatcgc accaccgttt ttgccgtctt tatgtttttt
     1321 tcgtcaaaaa cggcatttga cgcacaaacc gctattttcc cctgagacaa agtcgaataa
     1381 acagaaggta tttcgctttc ggggacaatg acccggatag aggacaatga ctcctcacac
     1441 ggctttatcg ccgcctttaa aacggtatcg caattgacaa gcggacgcat tttacagtcg
     1501 taaatgttgc cccttgacga agccaccgtc acgccgacgg tattttttga tgcggcgaca
     1561 tccgacacgt taccgccgca aagctccgcc atttttacaa aaagcacacc gaatgacaga
     1621 cacaacaaaa agaaaagg
//
LOCUS       DBMDVO010000317         1634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35786_length_1634_cov_1.775603, whole genome shotgun sequence.
ACCESSION   DBMDVO010000317 DBMDVO010000000
VERSION     DBMDVO010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35786_length_1634_cov_1.775603"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="ACZPE2_05075"
     CDS             complement(<1..210)
                     /locus_tag="ACZPE2_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1319254.1"
                     /translation="MNETILVVDDEREIADLLEIYLKNDGYTVHTCATGAEALRRVGS
                     EHIDLALLDVMLPDTDGFSLCRKIRE"
     gene            complement(462..773)
                     /locus_tag="ACZPE2_05080"
     CDS             complement(462..773)
                     /locus_tag="ACZPE2_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1319255.1"
                     /translation="MTEEFIRNRITELRLRKGVSEYQMSYDLGHSRGYVYNISSGKAL
                     PPMKEFLAICDYFGITPQQFFNGEESHPELIQKALAGMRQLDENDMLMLLGFINRLLK
                     K"
     gene            932..1096
                     /locus_tag="ACZPE2_05085"
     CDS             932..1096
                     /locus_tag="ACZPE2_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319256.1"
                     /translation="MAKTIKSIGFTQNDEALVEKITIYQKKNNLSFIAAVRELCETAL
                     KMETIVKKLG"
BASE COUNT          451 a          397 c          409 g          377 t
ORIGIN      
        1 ttcccggatc ttccggcaga gcgaaaagcc gtcggtgtcc ggcagcatca cgtcaagcag
       61 ggcgaggtcg atgtgctccg atcccacgcg gcgcagcgcc tccgcgccgg tggcgcaggt
      121 atggacggtg tagccgtcgt ttttgagata gatctccaga agatcggcga tctcgcgctc
      181 gtcgtccacg acgagaatgg tttcgttcat agctattctc ccaacgggca gtttggcgtt
      241 gagcccatta aaccacagtt tccgcccaaa cgcaagaaac cgccggggag gaggaaaatg
      301 gctccctcta tgagggagct gtcggcggca gagccgcaga ctgagggagt tgactccttc
      361 cgtctcgcct tcggcgagcc acctccctca aagagggagg cagcagaaaa aaggacgcgc
      421 agtcagaagc tgcgcgtccg ctctatcgtt aaggagcctt tttatttttt caaaagccgg
      481 ttgatgaagc cgagcagcat gagcatatcg ttctcgtcga gctgccgcat accggcgagc
      541 gccttctgga tcagctcggg atgggattcc tcaccattga aaaactgctg cggcgtgatg
      601 ccgaaataat cgcagattgc aagaaactcc ttcatcggcg gcagcgcctt gccggaggaa
      661 atgttgtata catacccgcg gctgtgccct aagtcgtagc tcatctgata ttccgaaacg
      721 cccttgcgca gccgaagctc ggtaatgcgg tttcggataa attcctccgt cattggtatc
      781 acctcatata gataataccg acaaagaaca attcaatagt aaataaatat ttgtcataac
      841 atattgcaat aactaaaaat atatgttatc atttgcaatg aatccaagaa tcttgtaaat
      901 aattactatt ccgacaaaag gaggcgagaa aatggcgaaa acgataaaga gtattgggtt
      961 cacgcagaat gatgaagcgc tggtagagaa gatcacgatt tatcagaaga aaaacaacct
     1021 atcctttatt gccgctgtca gagagctgtg cgaaacggcg ttgaaaatgg aaacaatcgt
     1081 aaaaaagctg gggtgaaaga tggcttgatc tgaaagacaa ttttgcttta tacatcatct
     1141 tttcacgcga gctatattaa ggaggaaaat atgtttataa cagtgtatgg aaaatacggt
     1201 ccccaaccac ctaaaaatca cgtaatcaat acaaacctta ttcagaaaat tcaaacttac
     1261 caacctctgt tcggaaaaga aaaatattat gacatctggg tacaaaccga cggatacggc
     1321 ccccaatatg tttaccgggt atcaagatcg gaggcaaaga agctttgtga cgcaattggg
     1381 atagacatcg tagacctata ataattattt tttcatgaaa aaaccgccgg gacagagctt
     1441 gtcccggcgg tcgtgcttat gaaaattatc agccgttgac catcttggcg acctcggcgg
     1501 cgaggtcgtc ctgacgcttc tcaatgcctt cgcccttctc aaaacggacg tagcccttga
     1561 cggtgacggg ggtgccgagc tccttggcga cggcggcgac atgctgctcg acgctgacct
     1621 tgtcgtcctt cacg
//
LOCUS       DBMDVO010000318         1634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35808_length_1634_cov_0.851272, whole genome shotgun sequence.
ACCESSION   DBMDVO010000318 DBMDVO010000000
VERSION     DBMDVO010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35808_length_1634_cov_0.851272"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>718
                     /locus_tag="ACZPE2_05090"
     CDS             <1..>718
                     /locus_tag="ACZPE2_05090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1319257.1"
                     /translation="DERRINSQTPPTEIEQFCDIQYIDDGLWQHRLDVYSKSGKLSHR
                     PVIIDIHGGGWMYGTKEINKNYCLVLAQNDYVVVNINYRLAPDFSVVDQLRDCFFAFK
                     WVSTNIEKYGGDLNNVFVTGDSAGGFLAAYSALLNSSEKLRKIFGVTESGLEFRACGL
                     TSPVCFMNTHTFQDVYYSRVKGKEFSESTLRGFENFDAVLNKGSMPPTFLVTSSGDDV
                     GRLPTIKMYNLLCGEGVKCKL"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1160..>1634
                     /locus_tag="ACZPE2_05095"
     CDS             1160..>1634
                     /locus_tag="ACZPE2_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1319258.1"
                     /translation="MDRFVLHSKYKPTGDQPEAIEKLTEGINAGYKEQTLLGVTGSGK
                     TFTMANIIANINRPTLVLAHNKTLAAQLCSEFREFFPENAVEYFVSYYDYYQPEAYIP
                     TTDTYIEKDSAINDEIDKLRHSATSSLSERRDVIIVASVSCIYSLGDPIDYRNMVI"
BASE COUNT          470 a          304 c          364 g          486 t
ORIGIN      
        1 gatgagagaa gaataaattc tcaaactccg cctaccgaaa tcgagcaatt ctgcgatatt
       61 caatacatag acgacgggtt atggcagcac agacttgatg tttactctaa atccggtaag
      121 ctctcacatc gtcctgtcat aattgacatt cacggaggtg ggtggatgta cgggacgaaa
      181 gaaataaata aaaattattg tcttgtcctt gcacaaaacg attatgttgt tgtcaatatt
      241 aattacagat tagctccgga tttttcagta gtcgaccaac tcagagattg ctttttcgcc
      301 tttaaatggg taagcacaaa catagagaaa tacggcggtg atttaaacaa tgtctttgta
      361 accggtgatt cggccggtgg atttttggcg gcatattccg cattgctgaa ttcgtctgaa
      421 aaactcagaa aaatattcgg agtcaccgaa agcggacttg agttcagagc gtgtggctta
      481 acttcgcctg tttgttttat gaatacgcac acttttcagg atgtgtatta cagccgtgtt
      541 aagggaaagg agttttcgga gagtacgctg cgcggatttg agaattttga tgccgtttta
      601 aataaggggt caatgccgcc gacgttcctt gtgacgagct cgggcgatga tgtcggtaga
      661 ctgcccacaa ttaagatgta taatttattg tgcggagagg gcgttaagtg taagcttgnn
      721 nnnnnnnntt ttcctgacct ttcaacaggg agagcaaggt ttgcgtccca ttgaaaatgc
      781 catgtgaagt gtaagcttga aaaccgcgat aaatataacg gaaaaaattt gcctcacgtc
      841 ttttcggtta tagacccgtt ttccgtcccg ggtaaggaaa caatttccga tatgcttgga
      901 tttttttctg attatgcaaa atcgcggtgc aaatagtgct tgaaaatgca gcttcaatac
      961 gagcgagtgc ggctgacgat ttattttaca gcagcaacgg tattatggct ctgatgagag
     1021 ccgatttgcc gatggtgctt attttatgac cgattcacaa tcttattttg gccgtcttat
     1081 tttaaataat ttggtttaca ataattattg tatatgatat aattttggta aacatttgtt
     1141 tggtaaatac gaggtgcgta tggatcgttt tgtgttacat tcaaaatata aaccgacagg
     1201 agatcagccc gaagcgattg aaaaattaac cgaggggata aatgccggtt acaaggagca
     1261 aacattactc ggcgttacgg gttccggcaa aacatttaca atggcaaata tcattgcaaa
     1321 cataaaccgt cctacgcttg tactcgctca caataaaacc ttagccgcac agctttgttc
     1381 tgaatttcgc gagtttttcc ccgaaaatgc ggtagagtat ttcgtatcat attatgacta
     1441 ttatcagccc gaagcatata ttcctactac cgatacctat atcgaaaaag attccgctat
     1501 taacgatgaa atagacaagc ttcgccattc ggcaacatcg tcgctttcgg agcggagaga
     1561 tgtcattata gtcgcaagcg tttcatgtat atattcgctc ggtgacccga tagattatag
     1621 aaatatggta attt
//
LOCUS       DBMDVO010000319         1631 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_35881_length_1631_cov_1.239216, whole genome shotgun sequence.
ACCESSION   DBMDVO010000319 DBMDVO010000000
VERSION     DBMDVO010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35881_length_1631_cov_1.239216"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="ACZPE2_05100"
     CDS             complement(<1..281)
                     /locus_tag="ACZPE2_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1319259.1"
                     /translation="MNNVLLHCCCAPCSLSCIDPLRNEGIEPIAFWYNPNIHPWKEYE
                     ARRNCLIDYASKINMRVLVEEDYGLREFVKNVVDDIDNRCTYCYETRLR"
     gene            complement(274..1629)
                     /locus_tag="ACZPE2_05105"
     CDS             complement(274..1629)
                     /locus_tag="ACZPE2_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1319260.1"
                     /translation="MLINAFFAMSEIAIISLNDAKMEKMAEDGDKKAKQVLKLTKNSS
                     NFLSTIQIGVTLAGFLTSAVAAQNFAVMLTDALAKTSVVKVIPVGAISVISTILITLI
                     MSYFSLVLGELAPKKFAMQKSEKISFAVTPILLGFSKITKPFVKLLSASTNLIVRMFG
                     LDPNADEETVTEEEIRMMVDVGQEKGVIEDSQKEMINNVFEFDDIDVADIMTHRTDME
                     CVDVEDSLQDVVKTSMEHGYSRIPVYEEDPDNVVGIIYIKDLLKYVGSNLPKTKTPKD
                     IMREVYCVPETKRCGELFSEMTAKHIQMAIVVDEYGGTAGLVTLEDIVEAIVGNIQDE
                     YDNEDEEISKINDTTFTIDGITDIEEVEEKLGIKLPEGDYDTLGGFIISLLGFLPVDG
                     DMNTVQYKNIRFTVLNVEERRIGKVKVEFLPEEQLGDEKTTDEKSEEKDKRKSGDKDD
                     E"
BASE COUNT          409 a          393 c          344 g          485 t
ORIGIN      
        1 ttgcgaagcc tcgtctcata gcaataggta cacctgttat ctatatcatc gacaacattt
       61 ttcacaaatt cgcgaagtcc gtaatcctct tcgaccaaaa ccctcatatt tattttcgaa
      121 gcatagtcga taaggcaatt acgccttgcc tcgtattctt tccaagggtg gatgtttgga
      181 ttataccaaa atgcgatcgg ctctattccc tcgtttcgca gagggtctat gcaggaaagc
      241 gagcacggcg cacagcagca gtgaagaagc acgttattca tcgtctttgt caccgctttt
      301 acgcttatcc ttttcctcgg acttttcatc agtcgttttt tcgtcgccga gctgttcctc
      361 cggcaaaaat tcgaccttga ccttacctat acggcgctcc tctacgttca acacggtaaa
      421 cctgatgttt ttatactgaa cggtgttcat atcgccgtca accggcaaaa agcccaaaag
      481 gctgattata aatccgccga gagtatcgta gtctccctcg ggcagcttta tgccgagttt
      541 ttcctcaacc tcttcgatat cggtaatacc gtctatcgta aaggtggtat cgtttatctt
      601 ggatatttct tcgtcctcgt tgtcatattc gtcctgaata ttaccgacga tagcctcaac
      661 tatatcctca agggttacaa ggccggcagt tccgccgtac tcgtcaacca ctattgccat
      721 ctgaatatgc ttagcggtca tttccgaaaa taattcgccg cagcgctttg tttcgggaac
      781 gcaatatacc tcgcgcatta tgtccttagg cgtttttgtt ttcggcagat tggacccgac
      841 atattttaaa aggtctttaa tgtatataat accgacaaca ttgtcggggt cttcctcata
      901 tacggggatt ctcgaatagc cgtgctccat tgaggttttg actacgtcct ggagagaatc
      961 ctcaacgtca acgcactcca tatcggtgcg gtgagtcatt atatcggcaa cgtcaatatc
     1021 atcaaattca aagacgttgt ttatcatttc tttttggctg tcctcaatta cgcccttttc
     1081 ctgaccgacg tcaaccatca tacggatttc ctcttctgtg acggtttcct catcagcgtt
     1141 ggggtcaaga ccgaacatac ggactataag gtttgtggaa gcagaaagaa gctttacaaa
     1201 cggctttgtt atcttactga agccgagaag tatcggagtg actgcaaagg agattttttc
     1261 agacttctgc atagcgaact ttttgggtgc aagctcgccg agaacgagtg aaaaataaga
     1321 cataataagc gttatgagta tagtcgaaat tacgcttatc gcaccgaccg gaattacctt
     1381 aacaacagat gttttcgcaa gagcgtcggt cagcattacc gcaaagttct gagccgcgac
     1441 agccgaagta aggaagccgg caagggttac gcctatctga atggtcgaga ggaagttgct
     1501 ggagtttttg gtaagcttta atacctgttt tgcctttttg tcgccgtcct ccgccatttt
     1561 ttccatcttg gcgtcattca gcgaaatgat agctatctcc gacattgcaa agaaagcgtt
     1621 gatcaaaata a
//
LOCUS       DBMDVO010000320         1627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36019_length_1627_cov_0.840105, whole genome shotgun sequence.
ACCESSION   DBMDVO010000320 DBMDVO010000000
VERSION     DBMDVO010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36019_length_1627_cov_0.840105"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="ACZPE2_05110"
     CDS             <1..372
                     /locus_tag="ACZPE2_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014006.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HIR1319261.1"
                     /translation="GIGCRKGCETVEESILAFLKVNGVSVYSLFAVATVDIKKNENGI
                     VEFCEKFDIPLLTFSAETLAAVEGEFTASEFVKKTVGVDNVCERAVCAVGAKITEKKT
                     ALNGTALALGRINTEIDFLKG"
     gene            379..1107
                     /gene="cobJ"
                     /locus_tag="ACZPE2_05115"
     CDS             379..1107
                     /gene="cobJ"
                     /locus_tag="ACZPE2_05115"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR1319262.1"
                     /translation="MKIYVVGIGAGSADGMTFEARNVLEKADVIVGYTVYAELVKKLF
                     PEKEYFVSAMKQEAERCRKAVEMAADGRNVAVVCSGDAGVYGMASLVYEIAAGYENVE
                     VSVVAGVSAMLSGGALVGAPLGHDFAVISLSDLLTPWEKIEKRLECASAADFAICLYN
                     PSSKKRHDYLMRACDIMLKHKNGDTVCAVAKNIGRDGEEYAIMSLAELRNYSADMFTT
                     VFIGNSMTKNIGGKMVTPRGYRNV"
     gene            1100..>1627
                     /locus_tag="ACZPE2_05120"
     CDS             1100..>1627
                     /locus_tag="ACZPE2_05120"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HIR1319263.1"
                     /translation="MYDVVLFGGTDEGHKIADFLSEQKVSYVVCVATEYGAELLDMKN
                     VRVGRMTAEEMADFFDENSVRLVIDATHPYAEFVTENIKKACKVKYIRVVREDVQADG
                     TYFENIKAAAEYLEKTTGNILITTGSKEIAKFSVVADRAFARVLPSEESLELCKNAGF
                     KMKNIICMQGPFSKAF"
BASE COUNT          484 a          248 c          468 g          427 t
ORIGIN      
        1 ggaatcggtt gcagaaaagg ctgcgaaact gtcgaagaaa gtatattggc atttttaaaa
       61 gtgaacggag tgtcggttta ctcgcttttt gcggttgcga cggtggatat aaagaaaaat
      121 gaaaatggca ttgttgaatt ttgtgaaaaa ttcgatatac cgcttttgac gttttcggct
      181 gaaactttgg cggcggttga gggagaattt acagcgtccg aatttgtgaa aaagactgtc
      241 ggagtcgaca atgtgtgcga aagagcggtt tgtgcggtcg gtgcgaaaat aacagaaaaa
      301 aagacggctt taaacggtac ggctttggca ctcggaagaa taaatacgga aattgatttt
      361 ttgaaaggtt aagtttgaat gaaaatttat gttgttggaa taggcgcagg aagtgctgac
      421 ggaatgactt ttgaggcgag aaatgtgctt gaaaaagccg atgtgattgt cggatatacg
      481 gtgtatgccg agcttgtgaa aaagctgttt cctgaaaaag aatatttcgt ttcggcgatg
      541 aaacaggagg cggagcgctg cagaaaagcg gttgaaatgg cggctgacgg cagaaatgtt
      601 gcagttgttt gcagcggtga tgcaggcgtt tacggtatgg cgtcacttgt gtatgaaatt
      661 gcggcaggct atgaaaatgt tgaagtgagc gttgtggcag gcgtgtcggc aatgcttagc
      721 ggcggtgcac ttgtgggagc accgttggga cacgattttg cggttataag ccttagcgat
      781 ttgctcaccc cttgggagaa aatcgaaaaa cgtcttgaat gtgcgtcggc ggctgatttc
      841 gcgatatgtc tgtacaatcc gtcaagtaaa aaacgtcacg attatttaat gcgcgcatgt
      901 gacataatgc tgaaacacaa aaacggtgat acggtgtgtg ccgttgcaaa aaatatcggt
      961 cgtgacggtg aggaatatgc gattatgagc cttgccgaac ttagaaatta cagcgcggat
     1021 atgtttacga ctgtgtttat cggtaattct atgacgaaaa atatcggcgg aaaaatggtt
     1081 acaccgagag ggtatagaaa tgtatgatgt agtattgttt ggcggtacag acgagggaca
     1141 caaaatcgcc gactttttga gtgagcaaaa agtcagttac gttgtgtgcg ttgcgacgga
     1201 atacggtgcg gaattgcttg atatgaaaaa cgtgcgcgtc ggaagaatga cggcggaaga
     1261 aatggctgat ttttttgacg aaaacagtgt tagattggtc attgacgcaa ctcatcctta
     1321 cgctgaattt gtgacagaaa acataaaaaa agcgtgtaaa gtgaagtata taagggttgt
     1381 gcgtgaggac gttcaggcag acgggacgta ttttgaaaat atcaaagcag ccgcagaata
     1441 ccttgaaaaa acaacaggaa acatacttat aacgacgggt tcaaaggaaa ttgcaaagtt
     1501 ttcggttgtt gcggacagag cgtttgcacg cgtactgccg tcggaggaat cgctggagct
     1561 gtgtaaaaat gcaggtttca agatgaaaaa tattatttgt atgcaaggac cgttttcaaa
     1621 agcgttt
//
LOCUS       DBMDVO010000321         1626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36041_length_1626_cov_1.137049, whole genome shotgun sequence.
ACCESSION   DBMDVO010000321 DBMDVO010000000
VERSION     DBMDVO010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36041_length_1626_cov_1.137049"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            69..>278
                     /locus_tag="ACZPE2_05125"
     CDS             69..>278
                     /locus_tag="ACZPE2_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1319264.1"
                     /translation="MEEYHKTGAKLFIQLTAGFGRSMAVNDLMEKVYCNKALRVLAKP
                     ILDVEYLCASASPNPNRWSDKVPSRA"
     assembly_gap    279..288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <289..>1626
                     /locus_tag="ACZPE2_05130"
     CDS             <289..>1626
                     /locus_tag="ACZPE2_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1319265.1"
                     /translation="IDEMIDAFAKTAKLCKEAGVDGIEIHAVHEGYLLDQFTMKYTNQ
                     RTDEYGGSFENRYRFPVEIVKAVKKACGKEFPVSLRYSVVSKTKGFRQGALPGEEYTE
                     VGRDMEESERAAKYLQDAGYDMLNCDNGTYDAWYWAHPPGYMPENCNLKDVEHIKKFV
                     DIPVVCAGRMTPQEGASAVKENKIDAVGFARQFLTDPAWVTKLMEDREEDIKPCICCH
                     NACFNMAHYEGVPNIQDMDDTSNMSRCALNPMTMQSKKYKIVTARVSKSVAVIGGGIG
                     GMETALVAAARGHAVTLYEKTDRLGGIFIEAAAPSFKEKDRDLIEWYRRAINNSSVTV
                     KLNSEIKRFSDISADEIIVATGSAANKPPIPGIEYAMDATTYLDGIDVGEDVAIIGGG
                     LTGCEIAYDLILKGKKPTVIEMKNDLIAVKGVCLANSSFLREMLKYKKAPVYL"
BASE COUNT          483 a          313 c          397 g          423 t
ORIGIN      
        1 tacgtgacac cttcggtggt aaatggttat atcagaatac ggggaaattc aagcagctta
       61 aagcttttat ggaagagtac cataaaaccg gtgcaaagct ttttatacag ttaacggcag
      121 gctttggtcg ttctatggca gtaaatgacc ttatggaaaa ggtatattgc aacaaagctt
      181 tacgtgtgct tgcaaagcct attcttgatg ttgagtacct ttgcgcaagc gcatctccca
      241 atccgaacag atggagcgat aaagtcccgt cgagagccnn nnnnnnnnaa atcgacgaaa
      301 tgattgacgc ttttgcgaaa accgctaaat tatgtaagga agcaggagtg gatggcattg
      361 aaattcacgc tgtgcatgag ggctatttgc ttgaccagtt tacgatgaaa tataccaatc
      421 agcgtacaga cgaatacggc ggttcttttg aaaaccgtta ccgtttccct gttgaaatag
      481 taaaagccgt aaaaaaggcg tgcggaaaag aattcccggt ttccctcaga tattcggttg
      541 tttcaaagac gaagggcttc cgccaagggg cattgcccgg cgaagagtat acggaagtcg
      601 gcagagatat ggaagaatct gagcgcgccg caaaatactt gcaggacgca ggttatgata
      661 tgctcaactg cgacaacggt acatatgatg cttggtattg ggctcatcct cccggatata
      721 tgccggagaa ctgtaacctt aaagatgtgg aacacataaa aaagtttgtc gacatacctg
      781 ttgtttgtgc cggcagaatg actcctcaag agggagcgag cgccgttaaa gaaaataaaa
      841 ttgacgctgt cggatttgca agacagtttc ttaccgatcc cgcatgggtt acaaaactga
      901 tggaagacag agaagaagat atcaagccgt gtatttgctg tcataacgcc tgctttaata
      961 tggcacatta tgagggtgtg ccgaatattc aagatatgga tgacacttcg aatatgtctc
     1021 gctgcgcgct taatccgatg acaatgcagt caaagaagta taagattgtc acggcaagag
     1081 tttcaaaatc cgttgctgtt atcggtggcg gtatcggagg tatggagact gctttagttg
     1141 ccgctgcacg cggtcatgcc gttactcttt atgaaaagac cgatagactc ggaggtatat
     1201 ttattgaagc tgccgctcct tcatttaagg agaaagaccg tgatttaatt gaatggtaca
     1261 ggcgagcaat aaataattcg tccgtaacag ttaagcttaa cagtgagatt aaaagatttt
     1321 cggatatttc tgcagacgag attattgttg ctaccggctc ggcagctaat aaacctccga
     1381 ttccgggaat tgagtatgca atggacgcaa caacatatct tgacggcata gatgtagggg
     1441 aggatgttgc aattatcggc ggcggtttaa ccggctgcga aattgcttac gacttaatcc
     1501 tcaaaggcaa aaagccgact gttatcgaaa tgaaaaatga ccttatagca gttaagggcg
     1561 tttgccttgc caattcatcg tttctcagag aaatgttaaa atacaaaaaa gctcctgttt
     1621 atctta
//
LOCUS       DBMDVO010000322         1622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36168_length_1622_cov_0.946746, whole genome shotgun sequence.
ACCESSION   DBMDVO010000322 DBMDVO010000000
VERSION     DBMDVO010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36168_length_1622_cov_0.946746"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..964
                     /locus_tag="ACZPE2_05135"
     CDS             83..964
                     /locus_tag="ACZPE2_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319266.1"
                     /translation="MSISTIIQHCLTGISLGGAYALIAIGYTMVYGILRLINFAHGDI
                     FMMAGYFMIFAMASFPWYIAIPITLLATVALGVVIEKVAYKPLRTAPRMSIMISAIGV
                     SYLLQNLATYLFTALPKGYPEIPVLKKIYQFGDVSASLVTFITPVLTIVLVIILMQLI
                     NHTKMGMAMRAVSKDYDTAQIMGIKINNVISYTFVIGSLLAGIGSILYFTDRMTVYPY
                     SGSLPGLKCFVAAVLGGIGSIPGAVVGGFILGVGETILTAAGYSTFSDAFTFVLLIIM
                     LLFRPTGIFGEKATDKV"
     gene            975..>1622
                     /locus_tag="ACZPE2_05140"
     CDS             975..>1622
                     /locus_tag="ACZPE2_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319267.1"
                     /translation="MKNIQTVSPNKKRNLVLSIVALAVIVAILFYLDANKAQHSYAIS
                     IIERSLIYAVVAVSMNLLTGFTGLFSLGQAGFMAIGAYTVAILTIPVDVRPSVYYMSG
                     ISPIIANLHMPFWAALILGGVLAAVVAALIGIPVLRLKSDYLAIATLGFAEIIRALLA
                     CPQMDRITNGSYGLKNIPGFSSIFTPFIISVICIGIMLLIINSSYGRAFKAIRENE"
BASE COUNT          446 a          352 c          313 g          511 t
ORIGIN      
        1 agtcttctct ccccactaat ttttttaaaa tcagtttaaa tttttaagta agaaagagaa
       61 caaccaacag ggggctttat ttatgagtat atcaacaata attcagcact gtctgacagg
      121 catttctctg ggtggtgctt acgcacttat cgccattggt tatacaatgg tttacggtat
      181 attgcgactg attaacttcg cacatggtga tatattcatg atggcaggtt attttatgat
      241 tttcgcaatg gcaagctttc cttggtatat tgctataccg ataacgcttt tggcaacagt
      301 tgcccttggc gtagttatcg aaaaagttgc ctataagccg ttacgtacag ctccgagaat
      361 gtctattatg atttcggcaa tcggtgtttc atatctgctt cagaatcttg caacatatct
      421 tttcacagct cttcctaagg gctatcccga aatacccgta ctcaaaaaaa tatatcagtt
      481 cggcgatgta tccgcatctc tcgttacatt cataactccg gtgcttacaa tcgtcctcgt
      541 tatcatactt atgcagctta taaatcatac aaaaatgggt atggctatgc gtgccgtttc
      601 aaaggactat gacacagcac agataatggg tatcaaaata aacaacgtaa taagctacac
      661 attcgttatc ggctcgcttc ttgccggtat cggttcaata ctttatttca cagaccgtat
      721 gaccgtttat ccttacagcg gctctctccc cggtcttaag tgcttcgtag ctgccgttct
      781 cggcggtatc ggaagtattc cgggcgcagt tgtcggcgga tttatactcg gtgtcggtga
      841 aacaatactt actgccgcag gatattcaac attcagcgac gcatttacat tcgtacttct
      901 tataataatg cttttattca gaccgacagg tatattcggc gaaaaagcaa cagacaaggt
      961 ttgaggtgat tactatgaaa aatatacaga cagtttcacc aaacaaaaaa agaaatttgg
     1021 ttttatcaat cgttgcactt gctgtaattg ttgccatact tttctatctt gacgcaaaca
     1081 aggcacagca cagctatgca atatcaatta tcgaaagaag ccttatatac gccgttgtag
     1141 ccgtatcaat gaaccttctt accggattta caggtctttt ctctctcggt caggcaggct
     1201 ttatggctat cggtgcatat acagttgcca tattaacaat tcccgttgat gtacgtccca
     1261 gtgtatacta catgagcggt ataagcccga taatagcaaa tcttcatatg ccgttctggg
     1321 ctgctcttat actcggcggt gttttagcgg cagttgttgc agcacttatc ggtattcccg
     1381 tactccgtct taagagcgac tatcttgcaa tcgcaacact cggctttgcc gaaatcatca
     1441 gagcgcttct tgcttgtcct cagatggaca gaataacaaa cggttcttac ggacttaaaa
     1501 atatccccgg attttcctct atattcactc cgtttataat ttcggtaata tgtataggaa
     1561 taatgctttt gataataaat tcatcatacg gacgtgcttt taaggctata agagaaaacg
     1621 ag
//
LOCUS       DBMDVO010000323         1617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36321_length_1617_cov_1.133905, whole genome shotgun sequence.
ACCESSION   DBMDVO010000323 DBMDVO010000000
VERSION     DBMDVO010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36321_length_1617_cov_1.133905"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /gene="def"
                     /locus_tag="ACZPE2_05145"
     CDS             <1..468
                     /gene="def"
                     /locus_tag="ACZPE2_05145"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1319268.1"
                     /translation="RVNDDPILRKVSRPVESFNAKLFKLLDDMRDTLYDADGCGLAAV
                     QVGMLKRVVVVDVGDGLIELINPEIVESDGVQYEIEGCLSLPGKSGVTVRPKTVKVRA
                     QNREGKWCLYKGEDLKARAFCHEIDHLDGHLYIERLAPEEVVERIRRESGRID"
     gene            480..1406
                     /gene="fmt"
                     /locus_tag="ACZPE2_05150"
     CDS             480..1406
                     /gene="fmt"
                     /locus_tag="ACZPE2_05150"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1319269.1"
                     /translation="MRIVFMGTPDFAVPCLKRLVDDGNEVVGVFTQPDKPKGRGYTLT
                     PPPVKVFAEENGIPVFQPKSMRDGEALEILKRLNPELNVVTAYGKILPPEILYLPKFC
                     SINIHASLLPKYRGAAPIQWCILNGEKETGVTSMLMNEGLDTGDMLISEKLPIDENMT
                     AGELHDKLSLLGADVLSKTIRALLDNSLKPIKQNDDESCYSPMLTKALCPIDFTKTID
                     EVHNKIRGLSPWPTATAVLGGKKVKLHSSEKTELKGGAPGEITVSHGEMLVACKDGKL
                     LKILELQPEGKKRMSAEAFLLGHKIQSGDSFK"
     gene            1419..>1617
                     /locus_tag="ACZPE2_05155"
     CDS             1419..>1617
                     /locus_tag="ACZPE2_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR1319270.1"
                     /translation="MPYYIDYYYFILIVPVLFISLIFQARVNFAYSKFSRLNNMNGLT
                     GADAARRVLDFYGIQNVRIEHI"
BASE COUNT          459 a          348 c          406 g          404 t
ORIGIN      
        1 agggtgaacg atgacccgat acttcgaaag gtcagtcgtc ccgttgaaag ctttaacgca
       61 aagctcttta aattacttga cgatatgaga gatactcttt acgatgccga cggctgcgga
      121 cttgccgccg ttcaggtggg aatgctcaaa agagtggtag ttgtagatgt cggcgacggt
      181 cttatagagc ttataaatcc ggaaatagtc gagagcgacg gagttcagta cgaaatagag
      241 ggttgccttt cgcttccggg taaaagcggc gttacggttc gaccgaaaac cgtaaaggta
      301 agagcgcaaa acagagaggg aaaatggtgc ctttataagg gcgaggacct taaagcgcgc
      361 gcattttgcc atgagataga ccatcttgac ggccatttgt atattgaacg tcttgcaccc
      421 gaggaagtag ttgagcgtat acgccgcgaa agcggacgaa ttgattgacg aggtaactta
      481 tgagaatagt ttttatgggg actcccgact tcgccgtgcc gtgtcttaaa agacttgtag
      541 atgacggtaa cgaggttgtc ggcgtgttta cacagcccga taagcctaag ggcaggggat
      601 atacgcttac tccgccgccc gttaaggttt ttgccgagga aaacggtata cctgtttttc
      661 agccgaaaag catgcgtgac ggagaagctc tcgaaattct taaaagactt aaccccgagc
      721 ttaacgtggt taccgcttac ggcaaaattt tacctcccga aatactgtat cttccgaagt
      781 tttgctcaat aaacatacac gcgtctcttc tgccgaaata tcgcggagct gccccgatac
      841 aatggtgcat attaaacggc gaaaaagaga ccggagttac atctatgctt atgaacgagg
      901 ggcttgacac cggagatatg ctcatttccg aaaagctccc gattgatgaa aatatgactg
      961 ctggcgagct tcacgataag ctgtcgctgc tcggcgcgga cgtcctttca aaaacaatca
     1021 gagctttgct tgacaacagc cttaaaccca taaagcaaaa cgatgatgag agctgttatt
     1081 cgcctatgct tacaaaggca ctttgcccga ttgattttac taaaacgatt gatgaagtgc
     1141 acaataagat aagaggtctc tcgccgtggc ccaccgcaac cgccgttttg ggcggcaaga
     1201 aggtcaagct tcattcttcc gaaaaaaccg agcttaaagg cggcgcaccg ggagaaatta
     1261 ctgtatctca cggcgaaatg cttgtcgcct gcaaggacgg taaactgctt aaaattcttg
     1321 agctccagcc ggagggcaaa aagaggatga gcgcagaagc ttttcttttg gggcataaaa
     1381 tacaaagcgg cgattctttt aaataagggt gaaaagaaat gccatattat attgattatt
     1441 attattttat tctgatagtc cccgtgctgt ttatttcgct gatattccag gcgagagtaa
     1501 atttcgcata ttcgaaattt tcccgcctca ataatatgaa cggcctgacg ggagccgatg
     1561 cggcaagacg tgtgcttgat ttttacggaa tacaaaatgt ccgtatagag catatca
//
LOCUS       DBMDVO010000324         1617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36322_length_1617_cov_1.121372, whole genome shotgun sequence.
ACCESSION   DBMDVO010000324 DBMDVO010000000
VERSION     DBMDVO010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36322_length_1617_cov_1.121372"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="ACZPE2_05160"
     CDS             <1..589
                     /locus_tag="ACZPE2_05160"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1319271.1"
                     /translation="FPVEDLKTLRKSDSYLQGHPNMNKVPGVDMSTGSLGQGISCAVG
                     MALGGKIDKENYRVYTILGDGEIEEGQVWEAAMFAGNHHLDNLTAFVDYNGLQIDGTI
                     DEVNSAYPIAEKFAAFKWNTIEIDGHDFDAIKKAVDDAKACKGMPTAVIMKTVKGKGV
                     SYMENAVNWHGAAPNEELYNKAMDELKSALAELEA"
     gene            593..>1177
                     /locus_tag="ACZPE2_05165"
     CDS             593..>1177
                     /locus_tag="ACZPE2_05165"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1319272.1"
                     /translation="MADIIKTATRDAYGKALIELGHKDKNVIVMDSDLAEATRTGKFK
                     KEFPDRFFDSGIAECDMMCIAAGLAATGHTVFASSFAMFAAGRAFEQIRNSIAYPNLN
                     VKIGATHAGISVGEDGASHQCCEDIALMRSIPNMVILNPADDVEARLCVLAAAEHDGP
                     VYMRFGRLAVPRVFDDNYNFEIGKGNVLVDGTDVT"
     assembly_gap    1178..1187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1188..1536
                     /locus_tag="ACZPE2_05170"
     CDS             <1188..1536
                     /locus_tag="ACZPE2_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1319273.1"
                     /translation="GLMVNEALIAAENLKADGISARVVNMATIKPIDSDIIVDSVKKT
                     GAVVTAEEHNIIGGLGSAVTEVVCEKCPAPVLRVGVEDVFGRSGPAVELLHIYGLDAA
                     HIEEKAKEAIKLK"
BASE COUNT          453 a          314 c          403 g          437 t
ORIGIN      
        1 ttttccggta gaggacctca aaaccctccg caagtccgat tcgtatttac agggacaccc
       61 caatatgaat aaggtgccgg gagttgatat gagcaccggt tctctcggac agggtatatc
      121 gtgcgctgtc ggtatggctc tcggcggaaa aatagacaaa gagaattaca gagtatacac
      181 aattctcggc gacggagaaa ttgaagaagg tcaggtttgg gaagcagcta tgtttgccgg
      241 caaccaccat cttgataatc tcaccgcatt tgttgactac aacggacttc agattgacgg
      301 taccattgat gaagttaact cggcttatcc gatagccgaa aagtttgccg ctttcaagtg
      361 gaataccatt gaaattgacg gccacgactt cgatgctata aaaaaggctg ttgatgatgc
      421 taaagcctgc aagggaatgc ctactgccgt tattatgaaa actgttaaag gtaaaggagt
      481 ttcctatatg gaaaatgccg tgaattggca cggagcagct cctaacgaag aattgtataa
      541 caaagccatg gatgaactta aatccgcttt ggcagaattg gaggcgtaaa caatggctga
      601 tataataaaa actgctacaa gagacgctta cggcaaagct cttattgagc tcgggcataa
      661 ggataaaaat gttattgtta tggattccga tcttgcagaa gctacaagaa ccggaaagtt
      721 taagaaggaa ttccccgata gattttttga ctccggcata gctgaatgtg atatgatgtg
      781 tattgctgcc ggtttagctg cgacaggtca taccgtattt gcaagttcgt ttgcaatgtt
      841 cgctgccggg agagcctttg agcaaatcag aaactctatt gcttatccta atctcaatgt
      901 taaaatcggt gccactcacg ccggtatttc ggttggcgag gatggtgcaa gccatcagtg
      961 ctgtgaggat attgcactta tgcgttccat acccaatatg gttattttaa atcctgccga
     1021 tgacgttgaa gctcgccttt gtgtgcttgc tgcggcagag catgacggac ctgtttatat
     1081 gagattcgga agacttgcgg ttccgagagt ttttgatgat aattataact tcgaaatcgg
     1141 taagggcaat gtgcttgttg acggaaccga tgttaccnnn nnnnnnnagg tcttatggta
     1201 aacgaggctc tcattgccgc tgagaattta aaggctgacg gcatatccgc cagagttgta
     1261 aatatggcaa ccattaagcc gattgacagc gatattatag ttgacagtgt taaaaagacc
     1321 ggtgctgttg ttactgccga agaacacaat attatcggtg gcctcggaag tgccgtaact
     1381 gaagttgtat gcgaaaagtg tcctgcacct gtcctcagag taggtgttga agatgttttc
     1441 ggcagatccg gtcccgctgt tgagcttttg catatttacg gccttgatgc cgctcacatt
     1501 gaagaaaaag caaaagaggc aatcaaactt aaatgagtga gattttcggg aaagacgctt
     1561 tatttagttt tgtaaatgag cactacggaa ttgagccgga ttacccgttt agtgacg
//
LOCUS       DBMDVO010000325         1616 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36351_length_1616_cov_1.368317, whole genome shotgun sequence.
ACCESSION   DBMDVO010000325 DBMDVO010000000
VERSION     DBMDVO010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36351_length_1616_cov_1.368317"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="ACZPE2_05175"
     CDS             complement(<1..967)
                     /locus_tag="ACZPE2_05175"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HIR1319274.1"
                     /translation="MKIIDLLKKEAIALNVSLTDKEEAIDKLISLHEKAGNLADKAAY
                     KEAILAREAQGSTAIGEGIAVPHAKSDSVKTPGLSAITVPNGVDYGAPDSKPSDILFM
                     IAAPVDGDLHLEILSRLMVMLMEPEFCAALRGAKTTDEFLEIIDKKEAEKYPEEAVKP
                     EVKPNGYRILAVTACPTGIAHTYMAAEALEKAGEKLGYPLKAETNGSGGAKNVLTKKE
                     IEECDGIIIAADKNVEMARFDGKPVVKTSVSNGINKPEELIKRIVDGKAAIYHADESE
                     KQPIDDDEKESGWHKLYKHLMNGVSHMLPFVVGGGVLIALGFLFDT"
     gene            complement(980..>1183)
                     /locus_tag="ACZPE2_05180"
     CDS             complement(980..>1183)
                     /locus_tag="ACZPE2_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1319275.1"
                     /translation="IGNAKGKLINSVGCGDSMVAGFTAGYIKTADYSYALRLGSACGN
                     ATAFSEKLATREEIERVFNAEYK"
     assembly_gap    1184..1193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1194..>1616)
                     /locus_tag="ACZPE2_05185"
     CDS             complement(<1194..>1616)
                     /locus_tag="ACZPE2_05185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR1319276.1"
                     /translation="DFNYLKNGDTRINVKIKADKEIDLNACGPEITKEDMQSFLRKLD
                     GIKSGDYLILAGSIPNTLPDDIYEQILERVGDRNINCVVDATGDLLKNVLKYRPFLIK
                     PNHHELGDLFSVQIKSDDDIVKYSKKLQEMGAKNVLVS"
BASE COUNT          337 a          400 c          366 g          503 t
ORIGIN      
        1 gcgtatcgaa caggaagccg agagctatga gaactccgcc gccgacaacg aacgggagca
       61 tatgcgaaac accgttcatg agatgcttat aaagtttgtg ccatccgctt tccttttcgt
      121 cgtcatcaat aggctgcttt tcactttcgt cggcgtgata tatcgcggct tttccgtcaa
      181 cgattctttt gataagctcc tcgggcttgt ttattccgtt tgaaaccgag gtctttacaa
      241 ccggcttgcc gtcaaaacgt gccatttcga cgtttttatc tgcggcaatg attattccgt
      301 cgcattcttc gatttccttt tttgtgagca cgtttttagc accgccggag ccgttggttt
      361 cggctttgag cggatagcct aatttttcgc ccgctttttc aagtgcctct gccgccatat
      421 aggtatgcgc tatgcccgtc ggacaggctg taactgcgag tattctgtaa ccattcggct
      481 ttacttcggg ttttaccgct tcctcggggt atttctccgc ttcctttttg tcgattattt
      541 ctaaaaattc gtccgtcgtt tttgcgcccc gtagagccgc gcaaaattcc ggttccataa
      601 gcattaccat aagacgggag agtatttcga gatgtaaatc tccgtcaacg ggagcggcaa
      661 tcataaacaa aatgtccgag ggcttggagt cgggagcacc gtaatcaacg ccgttcggaa
      721 ccgttatcgc cgaaagaccg ggagtcttta cgctgtccga ttttgcgtgc ggaacggcaa
      781 ttccttctcc gattgcggtc gagccctgag cctctcttgc aaggatagcc tccttgtacg
      841 ccgccttgtc ggcaagattt cctgcctttt cgtgcaggga aatcagcttg tcgattgcct
      901 cttccttgtc ggtgagagaa acattaagag caatcgcttc cttttttaat aagtcgatga
      961 ttttcataag tattcctcct tatttatact ccgcgttaaa gacgcgttca atttcttcac
     1021 gggttgcaag cttttctgaa aaggccgttg cgtttccgca cgccgagccg agtcttaaag
     1081 cgtaggaata gtcggcggtc ttgatatatc ctgccgtaaa gcctgctacc atactgtctc
     1141 cgcaaccgac ggagtttatg agtttgccct ttgcgttgcc tatnnnnnnn nnnttgaaac
     1201 aagcacgttt ttagcaccca tttcctgaag ctttttcgaa tatttcacaa tgtcgtcatc
     1261 actctttatc tggaccgaga acaggtcgcc gagctcgtga tgattcggct ttatcaaaaa
     1321 cggtctgtat ttgagtacgt ttttgagcaa atcgcccgtt gcgtcaacca cgcagtttat
     1381 atttctgtcg ccgactctct caagtatttg ctcgtaaatg tcatcgggga gggtattcgg
     1441 gatagagcct gcgaggataa gataatctcc gctctttatg ccgtcaagct ttcttaaaaa
     1501 gctctgcata tcctcttttg tgatttcggg gccgcaggcg ttcaaatcaa tttccttgtc
     1561 ggccttgatt ttgacgttta ttctcgtatc gccgttttta agataattaa agtcgc
//
LOCUS       DBMDVO010000326         1608 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36622_length_1608_cov_1.079628, whole genome shotgun sequence.
ACCESSION   DBMDVO010000326 DBMDVO010000000
VERSION     DBMDVO010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36622_length_1608_cov_1.079628"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            6..590
                     /locus_tag="ACZPE2_05190"
     CDS             6..590
                     /locus_tag="ACZPE2_05190"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1319277.1"
                     /translation="MKLLEDRIVKDGIVKPGNVLKVDSFLNHQMDISLFNDMGKEFKR
                     LFNDTPINKILTIEASGIGIACVAAQYFDNVPVVFAKKSQTVNIDGEVYSTKIESFTH
                     KRVYDVILSKKYLSSKDHVLIIDDFLANGCALNGLLDIAQKAGATVEGVGIAVEKGFQ
                     RGGELIRQKGIRVESLAIIESMDADSGNIVFKEQ"
     gene            664..>1608
                     /locus_tag="ACZPE2_05195"
     CDS             664..>1608
                     /locus_tag="ACZPE2_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1319278.1"
                     /translation="MKKVLSILLSVAMIVLCFAACGKTDGDKSSTGKAANVKVGFIFL
                     HDENSTYDKNFITAAEEACKKAGVEILKKTNISETQVCYDAAAELVDEGCNVIFADSF
                     GHEPYIIQAAKKFPEVQFLHATGTKAHTEGLSNYHNAFASIYEGRYLAGVAAGMKLNE
                     MISSGKITADKAKMGYVGAYTYAEVISGYTSFYLGAKSVCPSVTMEVTFTGSWYDETA
                     EKEAAQKLINNGCVLISQHADSMGAPTACEAAGVPNVSYNGSTVSACPNTFIVSSRID
                     WAPYLEYAIDCVKNGKAIDTDWTGTLSTGSVVLTDVNTK"
BASE COUNT          467 a          301 c          360 g          480 t
ORIGIN      
        1 ttttattgaa actgcttgaa gacagaattg taaaagacgg tattgttaag cccggcaatg
       61 tactcaaagt tgattctttt ttgaatcatc agatggacat ttctctgttt aatgatatgg
      121 gcaaggaatt caaacgtctt ttcaatgata cccctataaa taaaatactg accattgaag
      181 cctctggtat cggtatagct tgtgttgcgg cgcagtattt tgataatgtt cctgtggttt
      241 ttgcaaaaaa atctcaaact gttaatattg acggagaggt ttattccact aaaattgaat
      301 catttactca taaaagggtg tatgatgtta tactttcaaa gaaatatctt tcttcaaaag
      361 atcacgtcct tattattgat gactttttag ctaacgggtg cgctctgaat ggccttttgg
      421 atattgcaca aaaagccggt gctactgttg aaggcgtcgg tattgcggtt gaaaagggtt
      481 ttcaacgcgg tggcgaactt attaggcaaa aaggtatacg tgtcgagtct cttgccatta
      541 ttgagagcat ggatgcggat agcggaaaca tcgtatttaa ggaacagtga ccttttattt
      601 tgtatgatac agggggagag cttctgttca taatattatg tcaaaaaact ggaggaaaca
      661 caaatgaaaa aggttctcag cattcttttg tcagttgcta tgatcgttct ttgttttgca
      721 gcgtgcggca agacagacgg cgataaaagc tcgactggca aagcagcaaa tgtcaaagta
      781 ggattcatat tcctgcatga cgaaaactca acctatgaca agaactttat tactgcagct
      841 gaagaggctt gcaaaaaagc cggtgttgaa attcttaaaa agaccaatat cagtgaaaca
      901 caggtttgct atgatgcagc agcagagctt gttgacgaag gatgcaatgt catcttcgcg
      961 gacagcttcg gccatgagcc ttatataatt caggcagcta aaaagttccc ggaggttcag
     1021 ttcttacatg caacaggaac taaagctcat accgaaggcc tttctaatta tcataatgct
     1081 tttgcttcaa tatatgaagg cagatacctt gcgggtgttg ctgccggaat gaagcttaac
     1141 gaaatgattt cttccggtaa gattacagct gacaaagcca agatgggtta tgtcggtgct
     1201 tatacttacg cagaagttat ttcgggctac acttctttct atctcggtgc taagagcgtt
     1261 tgcccgtcag ttaccatgga ggttaccttt accggttctt ggtatgatga gactgccgag
     1321 aaagaagccg ctcagaagct tataaacaac ggttgcgtac ttatcagcca gcatgccgac
     1381 tcaatgggtg ctccgactgc ttgcgaagct gcaggtgttc ccaatgtttc atacaatggt
     1441 tcaacagtaa gtgcttgccc caacacattt attgtatctt caagaataga ttgggctcct
     1501 tatcttgagt atgctattga ttgcgttaaa aacggcaaag caattgacac cgattggaca
     1561 ggtactctca gcacaggatc tgttgttctt accgatgtta acactaag
//
LOCUS       DBMDVO010000327         1604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36730_length_1604_cov_1.541583, whole genome shotgun sequence.
ACCESSION   DBMDVO010000327 DBMDVO010000000
VERSION     DBMDVO010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36730_length_1604_cov_1.541583"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="ACZPE2_05200"
     CDS             <1..681
                     /locus_tag="ACZPE2_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319279.1"
                     /translation="FKNFRLKKQLYKEIFRGGIPSLARQGLASLAMVAMNRLAGGYGD
                     AAIAAMSIVSKIMNFFGAAVIGFGQGFQPVCGFNYGAKKYDRVKKAFWFSVKVTTVFM
                     TVVAVFGFILSPYVIALFRRDDTDVIAIGSFAFRAQCISLPLSGFIVTANMYLQTIGR
                     AKAATLTAMSRQFLFFVPALLLLVHFFGLTGVEISQAAADVAAFLFSMPICLKVLNIM
                     MTEENKIQ"
     gene            756..1523
                     /locus_tag="ACZPE2_05205"
     CDS             756..1523
                     /locus_tag="ACZPE2_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319280.1"
                     /translation="MSETLPTDALSKLGNDVFYDIRVLSAKVNSLLITAKDSPEKAEE
                     LYLSVLTTVCVYVENYLASRFSGLNSEYFERFCYSNIYGAEHRFTSLKKLAELSAYTL
                     NSVPQNMCDKNADIASAANRLFNVCRASVKAASAPVKYRDGKKYRVFKPSKRERLLLW
                     ELCGGNPEETAEKELSITYIARKNQSAASPKKYIRLSAWLFYTSIFSLLLVNLSGAFI
                     LLAVFSFLMSIMLAALYAVKSLSSHRKERNYREKNIR"
BASE COUNT          425 a          328 c          372 g          479 t
ORIGIN      
        1 tttaaaaact tccgattaaa aaaacagctc tataaagaaa tcttccgtgg cggaattccg
       61 tcccttgcga ggcagggtct cgcgtcgctt gccatggtgg ctatgaaccg acttgcaggc
      121 ggttacggcg atgccgcgat agccgctatg tccattgttt caaagataat gaattttttc
      181 ggagccgccg taataggctt cggtcaaggc ttccagcccg tatgcggctt taattacggt
      241 gcgaagaaat acgacagagt aaaaaaggcg ttttggttct ccgtcaaggt gaccaccgta
      301 tttatgaccg tggttgccgt tttcggcttt attctgtcac cgtatgttat agctttattc
      361 agacgcgatg acaccgatgt aattgcaatc ggttcgtttg cattcagggc acagtgcata
      421 tctcttccgc tttcgggatt tatagttacg gcgaatatgt atcttcagac cataggcagg
      481 gcaaaggcag ccaccttaac ggctatgtcg aggcagtttt tattttttgt tccggcgctg
      541 ttattgcttg tgcacttttt cggccttacg ggagtcgaga taagtcaagc agcagcagac
      601 gtggctgcat tcctgttttc aatgccgatt tgtctcaagg ttttaaatat aatgatgacc
      661 gaggagaata aaatacaata gcttgtgttg ccgaatggca tattgacttt atccgcgtaa
      721 tatgaaataa atacatatta cggagtcgat gatttatgag tgaaaccctt ccgaccgatg
      781 ctctgtcgaa gctcggaaat gatgtctttt atgatatacg tgtgttatcc gcaaaagtga
      841 attccttgct tataactgcg aaagattcgc ccgaaaaagc cgaagagctt tacctgtctg
      901 tgcttacaac ggtgtgcgtc tacgtcgaaa attatcttgc aagccgcttt tccgggctga
      961 attcggaata ttttgaacgc ttctgctatt ctaatatcta cggagcggaa cacagattta
     1021 cctctttaaa aaagcttgcg gaattgtcgg cgtatacatt aaattccgtg ccgcaaaata
     1081 tgtgtgataa aaatgccgat atagcttcgg cggcaaacag actttttaat gtttgccgtg
     1141 cttcggtaaa ggcagcgtct gctccggtaa aatatcgaga cggtaaaaaa tacagagttt
     1201 ttaagccgtc aaaacgcgaa cgactgctgc tttgggagct ctgcggagga aacccggaag
     1261 aaacggctga aaaggaatta agcattacat atattgcaag aaaaaaccaa tccgccgcgt
     1321 cgccgaaaaa atatatacgc ctttcggctt ggttgtttta tacttcgatt ttttcattgc
     1381 tgcttgtaaa tttaagcgga gcattcatat tgcttgcggt attttctttt cttatgagca
     1441 ttatgcttgc cgctttgtat gcggtaaagt cgctctcctc tcatcgtaag gagcgcaatt
     1501 accgcgaaaa aaatatacgt tgaaaaaaca tattttgtct tgtctcggat tgctttctaa
     1561 tgcgtggcgg gactgtttat atcggacaga aaggatatgg aaaa
//
LOCUS       DBMDVO010000328         1604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36741_length_1604_cov_1.158350, whole genome shotgun sequence.
ACCESSION   DBMDVO010000328 DBMDVO010000000
VERSION     DBMDVO010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36741_length_1604_cov_1.158350"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="ACZPE2_05210"
     CDS             <1..1582
                     /locus_tag="ACZPE2_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336605.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR1319281.1"
                     /translation="SLLGGCCGTTPEHLKEAVKLCKDIPVLPVKQKNITAVTSYSKTA
                     VIGERPIIIGERINPTGKKKLKAALVSGDSGYIFSEALSQVECGADVLDVNVGLPEID
                     EPKVMCDTVRGIQGITNLPLQIDTSDPAAMEQALRIYNGKPLINSVNGKKESMEKIFP
                     LAKKYGGVVVCLTLDESGIPESAEGRIKIAEKIINTAKEYGIDKKDLIIDTLAMTVST
                     GAENAKRTLQALSYVRNTLGVNTVLGVSNISFGLPKRDNINAAFLTLAMGSGLSAGII
                     NPKSDSMMNAFYSYCALNGYDENFETYISKTSDSAPTAKNDKTDLKTAVINGLSGAAG
                     ENARELLKTEQPLDIINLYLIPALDIVGKGFEDGTIFLPQLLMSADAAKAAFDEIKAS
                     IPAEANGSEKKNKVVIATVKGDIHDIGKNIVKVLLENYGFDVIDLGKDVNPEIIVDTA
                     IKEKVKLVGLSALMTTTVVNMEETIRLLRKRYPECKVMVGGAVMTQDYADRIGADSYA
                     KDAMGAVRYAERLFSDEQ"
BASE COUNT          500 a          330 c          404 g          370 t
ORIGIN      
        1 ctcgctgctc ggcggttgct gcggaactac gcctgagcac ctgaaagagg ctgtaaagct
       61 gtgcaaggat atccctgttt tacctgtaaa gcaaaaaaac attacagcag ttacatcata
      121 ctcaaaaacg gctgtaatag gcgaaagacc tataataatc ggtgaaagaa taaatccgac
      181 gggcaagaaa aaacttaaag cggcactcgt ttcgggcgac agcggatata tcttttccga
      241 ggctctgtct caggtcgaat gcggagcgga tgtacttgac gttaacgtcg gtttgcccga
      301 aatcgatgag ccgaaagtga tgtgcgatac tgttaggggc atacagggaa ttacaaatct
      361 tccgcttcaa atagacacct ccgatcccgc ggcaatggag caggcacttc gcatatataa
      421 cggcaagccg cttataaatt cggtaaacgg caaaaaagag agtatggaaa aaatctttcc
      481 gctcgctaaa aaatacggcg gagtcgtggt ttgccttacg cttgatgaaa gcggtattcc
      541 cgaaagtgcc gagggcagaa tcaaaatagc cgagaaaata ataaataccg caaaggaata
      601 cggtatagat aaaaaggacc tcataataga tacgcttgct atgaccgtaa gcaccggggc
      661 ggaaaacgca aagagaacgt tacaggctct gtcatatgtt cgcaacactc tcggcgtaaa
      721 tacggttttg ggagtttcaa atatttcctt tggccttccg aaacgcgata acataaacgc
      781 ggcattttta actcttgcca tgggcagcgg actttcggca ggtattataa atccgaaatc
      841 cgacagtatg atgaatgctt tttattccta ttgcgcgctt aacgggtacg atgaaaattt
      901 tgagacgtac ataagcaaga cgtcggattc ggcaccgacg gcaaaaaatg ataaaaccga
      961 ccttaaaacc gcggttataa acgggctttc cggagcggca ggggagaatg caagagagct
     1021 tttaaagacg gaacagcctc ttgacattat aaatctatat ttgatacctg cacttgatat
     1081 tgtcggtaaa ggctttgagg acggaacaat ctttttgcct cagcttctta tgagtgccga
     1141 tgcggcaaag gcggcatttg atgaaatcaa ggcaagcata cctgccgaag cgaacggcag
     1201 cgagaaaaag aataaggtag ttatagccac cgtcaagggc gatattcacg acatagggaa
     1261 aaacattgtt aaggttttgc ttgaaaacta cggctttgac gtaatcgacc tcggcaaaga
     1321 tgttaacccg gaaattatag ttgatacagc aataaaagag aaggtcaagc tcgtgggtct
     1381 gagtgcgctt atgactacaa cggttgtgaa tatggaggag accattcgcc ttttaagaaa
     1441 acgctatccc gaatgtaagg tgatggtcgg cggagccgtt atgactcagg attatgccga
     1501 cagaataggc gccgattctt acgcaaagga cgccatgggc gctgtgcgat acgcggaaag
     1561 actgttttcc gatgagcaat gaaataagct tgatacggca aagt
//
LOCUS       DBMDVO010000329         1597 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_36980_length_1597_cov_1.211230, whole genome shotgun sequence.
ACCESSION   DBMDVO010000329 DBMDVO010000000
VERSION     DBMDVO010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36980_length_1597_cov_1.211230"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="ACZPE2_05215"
     CDS             complement(<1..550)
                     /locus_tag="ACZPE2_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319282.1"
                     /translation="MKAETKSPLKKNGAADSKLSGRIWFNICLFGFTGQMAWTLENMY
                     FNTFLYNTVYEGGKVTGSLSSMTAIKLMVAFSAATAVITTFIMGNLSDRVNKRKIFIS
                     LGYIIWGITTGAFGFITKDNIGSLFGISDSYKVITATAVTVIVMDCVMTFFGSTSNDS
                     AFNAWITDITTPKNRATAESALA"
     gene            827..>1597
                     /locus_tag="ACZPE2_05220"
     CDS             827..>1597
                     /locus_tag="ACZPE2_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319283.1"
                     /translation="MENKQREKSAAEAYREERKQRMAKAAKKQTKKNPKAAKAGRIVG
                     KVIAIVLAVAICLAAVGGILNFFGVPQKVLKAAKFGSEKDTVATYNYYYMTLYNYYYN
                     MSNQYDTYYGKGMGKMYTGYDTSKTPMDQEYTGDTSSLNTDEEIKTWADFLRVSALNY
                     MQSYTAYADLARKNGLTLNDEEKAEIDERVSSIKKSADNNDFSLDRYIQKIYGKGVTE
                     KVLRAALEDSTLASKYAQQKQSEISDAITNDEIVAEYEA"
BASE COUNT          493 a          307 c          344 g          453 t
ORIGIN      
        1 tggcaagcgc gctctcggcg gtagctctgt ttttcggagt tgtaatgtcc gttatccatg
       61 cattgaaggc cgagtcgtta cttgttgagc cgaaaaatgt cataacgcag tccataacga
      121 ttacagttac ggcggtggcg gtgataacct tataagaatc ggagatgccg aacaggctgc
      181 ctatgttgtc ttttgttata aaaccgaatg ctccggttgt aataccccaa attatgtagc
      241 cgagagaaat aaatattttt ctcttgttta ctctgtctga cagattaccc attataaatg
      301 tggtaataac cgccgttgcg gcactgaatg ctaccataag ctttattgcc gtcattgacg
      361 agagcgagcc ggttactttc ccaccctcgt aaaccgtgtt gtaaaggaat gtgttgaaat
      421 acatattttc aagcgtccag gccatttgac ccgtaaatcc gaaaagacaa atgttgaacc
      481 aaattcttcc ggataatttg ctgtctgctg cgccgttttt cttgagcgga ctttttgtct
      541 cagctttcat tatctccctc ttttcaatgt atgaaactgt ttgtgacaat ttcatataaa
      601 atatcattca tctattataa ttgtgtgcaa aaagtatgtc aagaaatata tttgacaact
      661 gtataaaagc aatcggcttc ttttgaccga cgccacaaaa ctgttaacaa tttttaaatt
      721 tgctaaaaat ttgtcttttt gtcttgtaac tttttaaaaa atagtataaa atgtatctgt
      781 ttatcagtat ttcactgaag atcattttgt aaaggatgaa aacttgatgg aaaacaagca
      841 acgtgaaaaa agcgctgccg aagcgtacag agaagaacgt aagcagcgta tggccaaggc
      901 tgccaaaaag cagactaaaa agaatcctaa ggcagcaaag gccggcagaa tagtaggaaa
      961 ggttatagct attgtccttg ccgtggctat ttgtcttgcg gccgttggcg gtatactcaa
     1021 tttcttcggt gttccgcaaa aggtgcttaa agccgctaaa ttcggctctg aaaaagatac
     1081 cgtggcaaca tataactact attatatgac gctctataat tattattata atatgtcgaa
     1141 tcagtatgat acatattacg gcaagggtat gggcaagatg tataccggct atgatacatc
     1201 aaaaactccc atggatcagg aatatacagg tgatacttca agtcttaata cagatgaaga
     1261 aatcaagact tgggcagatt ttctcagggt ttcggcactg aattatatgc agtcctatac
     1321 ggcatatgcg gatttggcga ggaaaaacgg tcttactctc aatgacgaag aaaaggccga
     1381 gattgacgag agagtatcaa gcatcaagaa gagtgccgac aacaacgatt tttcgcttga
     1441 ccgctatatt cagaaaatat acggcaaggg cgtaaccgaa aaggttctca gagccgctct
     1501 tgaagacagc acgcttgcat ccaagtatgc acagcagaag cagtccgaaa tatcggatgc
     1561 gataacgaat gatgagatag tagccgagta tgaggct
//
LOCUS       DBMDVO010000330         1595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37040_length_1595_cov_1.384873, whole genome shotgun sequence.
ACCESSION   DBMDVO010000330 DBMDVO010000000
VERSION     DBMDVO010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37040_length_1595_cov_1.384873"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="ACZPE2_05225"
     CDS             <1..528
                     /locus_tag="ACZPE2_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HIR1319284.1"
                     /translation="CDTLSVYLAVGGALPLCAGMIIDVPSDIKTVGKFCAIAGVIIIV
                     LTAGRESKSFFGKIGSGLYAVYNTISGYLSDILSYARLLALGLVTGIIGSVVNMMGAL
                     PTNKVAKAIVLAVVFVFGHAVNFGINVIGAYVHTNRLQYVEFFSKFYNGGGKKFTPLK
                     ANSKTFKFKEEISNG"
     gene            521..1000
                     /locus_tag="ACZPE2_05230"
     CDS             521..1000
                     /locus_tag="ACZPE2_05230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIR1319285.1"
                     /translation="MDNMGLVLAIVGAALAALLSGLGSAKGVGLVGEAAAGLLTEDLS
                     KFTKVLILQILPGTQGLYGFITAIMVLVKTKLLSGSPAELTVSQGLMLLGACLPMAIV
                     GYFSAIAQARVAASGVSVVAKKPEGQSKAMVLAAMVETYAVLALLISILMILNLNIG"
     gene            1013..>1595
                     /locus_tag="ACZPE2_05235"
     CDS             1013..>1595
                     /locus_tag="ACZPE2_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1319286.1"
                     /translation="MTGLDKIISEIGSDSSQRIKEISDEANKKAEEISLAAEKKVSEM
                     KAQSEERTAAEVASVLASAKSSAELKKKQTVLEGKIEAIEDMLGRAVDVLKALPKREY
                     FEMLKELAVKNAISGDGVMRFSKDDTAKLPQNFISNVNSALSGKGSVSLGEPCDIDSG
                     FMLVYGDIDVNCTFSSLVSEKRDELFDELNKLLF"
BASE COUNT          402 a          356 c          415 g          422 t
ORIGIN      
        1 tgcgatacgc tgtccgttta ccttgctgtc ggcggtgcgc ttccgctttg cgccggtatg
       61 attatagacg ttccgtcgga tataaaaacg gtcggcaaat tttgcgcaat agccggcgta
      121 atcataatag tcctcacggc aggcagggag tcaaagtcgt tcttcggcaa aatcggctcc
      181 ggactttacg ccgtgtacaa cacgatttcg ggctatctca gcgatattct ttcatacgca
      241 aggcttttgg cactcggact tgttaccggc ataatcggca gcgtagtcaa tatgatgggt
      301 gctttgccga ccaataaggt cgcgaaggca atagttctcg cggttgtgtt cgttttcgga
      361 cacgcggtaa acttcggaat aaacgtcata ggcgcttatg ttcatacaaa ccgcttgcag
      421 tacgtggaat ttttctcgaa attctataac ggcggcggaa agaaatttac ccctcttaaa
      481 gcaaattcca aaactttcaa attcaaggag gaaatcagta atggataata tgggtttggt
      541 attggctatc gtcggagcgg cgttagcggc acttctcagc ggactcggtt cggcaaaggg
      601 cgtaggcctt gtaggtgagg ccgccgcagg tcttctcacg gaggatttgt caaaattcac
      661 taaggtgctt attcttcaga ttcttcccgg tacacagggc ctttacggct tcattaccgc
      721 aataatggtt cttgttaaaa caaagcttct ctcgggttct cccgccgagc ttacggtttc
      781 acagggactt atgcttctcg gcgcgtgcct gcctatggca atagtcggtt atttctcggc
      841 aattgctcag gcaagagttg cggcttcggg tgtttcggta gttgcaaaga agcccgaggg
      901 acagagtaag gcaatggttc ttgccgcaat ggttgagaca tacgccgttc tcgctcttct
      961 tatttcgatt cttatgattt tgaacctcaa catcggttaa gggtgaagct ttatgacggg
     1021 attagataaa attatttcag aaataggctc ggactcgtca cagcgtataa aagaaatttc
     1081 cgatgaagca aacaaaaagg ctgaggagat atcccttgcc gccgaaaaaa aggtttccga
     1141 gatgaaagct cagagcgaag agaggactgc cgcagaggtt gcctccgttt tggcttcagc
     1201 caaaagctcc gccgagctaa aaaagaagca gacggttctc gagggtaaaa tcgaggccat
     1261 agaggatatg ctcggcaggg cggtagatgt cttaaaggct ctcccgaaaa gagagtattt
     1321 tgaaatgctg aaggagcttg ccgttaaaaa tgcgatctcg ggagacggcg ttatgcgttt
     1381 ctcgaaagat gacaccgcaa agctgccgca gaatttcata agcaacgtaa attccgctct
     1441 ttccggtaag ggcagcgtct ctctcggaga gccgtgcgac atagacagcg gatttatgct
     1501 tgtttacggc gacattgacg taaactgcac cttttcgtcg cttgtttcgg aaaagcgcga
     1561 cgagcttttt gacgagctta acaaattact tttta
//
LOCUS       DBMDVO010000331         1594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37080_length_1594_cov_0.964501, whole genome shotgun sequence.
ACCESSION   DBMDVO010000331 DBMDVO010000000
VERSION     DBMDVO010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37080_length_1594_cov_0.964501"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="ACZPE2_05240"
     CDS             <1..520
                     /locus_tag="ACZPE2_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319287.1"
                     /translation="AVLGTAVFFCGLGFYKTNVVAISVLLMLQNFFFAFFQTVNNVIP
                     TEMIGDTVDYMEWKTGKRNEGVSFSVLTFVGKLTGSLSTSIGTALLPLIGLTFTKDTV
                     GNSVAVKGEHTDLWIWALFILIPKLLGLITLIPYAFYNLNGEKLKQIREDLKNRREEK
                     AKVQAIGGNENE"
     gene            513..1136
                     /locus_tag="ACZPE2_05245"
     CDS             513..1136
                     /locus_tag="ACZPE2_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319288.1"
                     /translation="MSNKCPKCGAKLSPFYLKPNCPSCGVNIVQYGFDERLESDKIRA
                     EKEWERFDNFLNGLKKSSIGSPIALVRLISFFLPIVALLIPVYKVNGAGINLISIIKS
                     IISDSASVFQNKAMLLCFISFAAVILTSLVCAVISLFSYTKNGYKRNIILSGIQICTF
                     IALSTAAVINGASIFAGAAAVILFQILTLYLHKKYKKSIEENKNNEQ"
     gene            1126..>1594
                     /locus_tag="ACZPE2_05250"
     CDS             1126..>1594
                     /locus_tag="ACZPE2_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013256.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1319289.1"
                     /translation="MSNKKKIYTVATAHLDTVWSWDFETTVSKYIYNTLVDNFKLFKK
                     YPTYTFSFEGSYRYELMEEYYPELFDKMKEYIKQGRWNVCGSSFENGDVNIPSPEALF
                     RNILFGNSYFDKKFGKRSVDIYLPDCFGFGWALPSIASHANLMGFTTQKLAWGS"
BASE COUNT          497 a          320 c          334 g          443 t
ORIGIN      
        1 cgctgttttg ggcacggcag tattcttctg cggcctcgga ttttataaaa caaatgttgt
       61 tgccatctct gttctgctta tgctccaaaa ctttttcttt gcctttttcc aaactgtcaa
      121 caatgtcatt cctactgaga tgataggcga cacggtagac tatatggaat ggaaaacggg
      181 aaaaagaaat gagggagtca gcttttccgt actcaccttt gtaggcaagc tgaccggttc
      241 tctgtccacc tctatcggta ctgcactgct gcctctgata ggtttaactt tcactaaaga
      301 cacagtcggt aattcggtag ccgtaaaagg agaacacact gacttgtgga tatgggcgct
      361 gtttattctg atacctaaac tgttaggtct cattactctt attccgtacg cattctacaa
      421 tctgaatggc gaaaagctaa agcaaattcg tgaagactta aaaaacagac gagaagaaaa
      481 agcaaaagtt caggcgatag gaggcaacga aaatgagtaa caaatgtccc aaatgcggtg
      541 ccaagttaag cccgttttat ctcaagccaa actgtccgag ctgcggcgtg aatatcgtgc
      601 aatatggctt tgacgaaagg ctcgaaagcg acaaaataag ggcagaaaaa gaatgggaac
      661 gattcgataa ttttttgaac ggactcaaga agtcctccat cggctcgccc atagcgttag
      721 tcagactcat atcgtttttt ctgccgatag tcgcactgct gatacctgta tataaagtga
      781 acggtgcagg cataaatctt atatcaataa ttaaaagtat catttcggat tccgcatcgg
      841 tgttccaaaa caaagcgatg ctgctttgct ttatatcatt tgcggcagtt attttgactt
      901 cgctggtttg tgcggttata tccctgtttt cttatacaaa aaacggatat aagcgaaata
      961 taattttgtc gggtatacaa atctgcacat ttatagcttt aagcacagcc gctgttataa
     1021 acggggcaag catttttgca ggggcggcag cagtaattct gttccaaata ctcacattgt
     1081 acctacacaa aaagtataaa aaaagtatcg aggaaaataa aaacaatgag caataaaaag
     1141 aaaatctata cagtcgcaac cgcacatctc gacaccgttt ggagctggga ttttgaaact
     1201 actgtaagca aatatatcta caacacactt gttgacaact tcaagttgtt caaaaaatat
     1261 cctacataca ctttttcatt tgaaggctca tacagatacg agctgatgga ggagtattat
     1321 ccggaacttt ttgacaaaat gaaagaatat ataaaacaag gaagatggaa tgtttgcggt
     1381 tcatcatttg aaaacggaga cgtaaacatt ccgtcacctg aggctttgtt tagaaacatt
     1441 cttttcggca actcatattt tgacaaaaaa ttcggaaaaa gaagcgttga tatatatttg
     1501 ccggactgct ttggcttcgg ctgggcattg ccgagcatcg caagtcacgc caatctgatg
     1561 ggattcacca cccaaaagct cgcttggggc agtg
//
LOCUS       DBMDVO010000332         1593 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37104_length_1593_cov_1.186327, whole genome shotgun sequence.
ACCESSION   DBMDVO010000332 DBMDVO010000000
VERSION     DBMDVO010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37104_length_1593_cov_1.186327"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..366
                     /locus_tag="ACZPE2_05255"
     CDS             136..366
                     /locus_tag="ACZPE2_05255"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1319290.1"
                     /translation="MRFSAFCEYTNDEEGHRFAEYGICCKTGSTDVKIRGITLEKSEI
                     NELLAMLRSGRPEMCHIQYIIEDFITLKCSVI"
     gene            562..1332
                     /locus_tag="ACZPE2_05260"
     CDS             562..1332
                     /locus_tag="ACZPE2_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027796.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR1319291.1"
                     /translation="MDFKSDSFMYGLGICIVLFVIAQSVFFLVRAVRRAKALGIETST
                     VKKTISSSAVFTVAPALAIVATVITLAKALGIVLPWIRLSVIGAIQYEVPAAEAAVEA
                     FGISSGISQEITDPKIFATAAWVMTIGSVMPLVLVPFVLKKIQKGMNKVASKDSRWSD
                     LMSAAAFIGLISAFIGRALAGSGDKFVVGDGAGVMSVAALLSSMLFMFILEKLSDKYN
                     INWLKPFAMPFSMILAMILVMILAKVLPSGIATFEWRG"
     gene            1335..>1593
                     /locus_tag="ACZPE2_05265"
     CDS             1335..>1593
                     /locus_tag="ACZPE2_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319292.1"
                     /translation="MKKKRSYSDSVHFYGIIWDIAALLVFMMIPVSICMHLNVWPEAK
                     YVLKGLLPVAMIFYPSAVIEVLTYTPLLGAGGTYLGFVTGNI"
BASE COUNT          401 a          311 c          382 g          499 t
ORIGIN      
        1 aaaaaacgca ctttcgctat tgtcagtgaa ggtgcttttt atctttccgg cagaatatat
       61 ctccgtgttc cgtattttgc ggcacgattc ttgcggcttt tgaatcgggc ttttaaataa
      121 tcggagggag aatatttgcg gttttctgct ttttgtgagt atacaaatga tgaggaaggt
      181 catcggtttg ccgagtacgg aatatgctgt aaaacaggtt cgaccgatgt taaaatacgt
      241 ggcataactc tcgaaaagag cgaaatcaac gagctgctgg ctatgctgag atccggcaga
      301 ccggagatgt gtcatataca atatataata gaagatttta taacgctgaa gtgcagcgtc
      361 atatagtgcg ataagcaatc agcgaaaatg tcgttttgcg acacaggctc ctataatccc
      421 aatttaataa tcggtcgtca ccgacatcgg cgtgatgtaa aagcagatcc tcaaattgtt
      481 tgtgcaattt gaggattttt tattgtgctt tataatattt tatgttatta ttaaatgaaa
      541 ttttttattg ggggaatctt aatggatttt aaatcggatt cgtttatgta tggattgggt
      601 atatgtattg tccttttcgt cattgcacag tcggtgtttt ttcttgtgcg cgccgtgagg
      661 agggcaaaag cactcggaat tgagacttcg accgtcaaaa aaaccatatc atccagtgcc
      721 gttttcacgg tagctccggc tcttgcaata gttgctacgg taataactct tgctaaggct
      781 ctcggtatag ttcttccgtg gatacgtctt tcggtaatcg gcgcaataca gtatgaggta
      841 ccggccgccg aggcagccgt tgaggcattc ggaatttcga gcggaatttc acaggagata
      901 accgatccca agatttttgc aactgctgca tgggttatga ctatcggcag cgttatgccg
      961 cttgtgcttg ttccgtttgt actcaaaaaa atacaaaagg gtatgaataa ggtcgcgagt
     1021 aaggacagca gatggtccga ccttatgtct gccgcggctt ttataggtct tatttccgcc
     1081 tttatcggca gagcactcgc aggctccggt gataaattcg tagtaggcga cggtgccggc
     1141 gtaatgtccg tagcggcact tctttcgtca atgctcttta tgttcattct cgaaaagctg
     1201 tccgataagt ataatataaa ttggctcaag ccttttgcca tgccgtttag tatgatactt
     1261 gcaatgatac tcgtaatgat acttgccaag gttcttccgt cgggcattgc gacatttgaa
     1321 tggaggggtt aaggatgaag aaaaagagaa gctactctga cagcgttcat ttttacggaa
     1381 ttatatggga tattgccgcc ttgctcgtct ttatgatgat tccggtttct atttgcatgc
     1441 atttgaatgt gtggcccgag gctaaatatg tactcaaggg acttttaccc gttgccatga
     1501 tattttaccc gtcggcggtt atagaggttc ttacatatac ccccttactc ggtgccggcg
     1561 gaacatacct cggctttgta accggtaata tta
//
LOCUS       DBMDVO010000333         1591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37183_length_1591_cov_0.698658, whole genome shotgun sequence.
ACCESSION   DBMDVO010000333 DBMDVO010000000
VERSION     DBMDVO010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37183_length_1591_cov_0.698658"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>286
                     /locus_tag="ACZPE2_05270"
     CDS             <1..>286
                     /locus_tag="ACZPE2_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019688748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1319293.1"
                     /translation="DIHVIPDMVRNFIDGYDVVYGVRNKRDTDTFFKRTTAVGFYKLM
                     QFMGVNIVFNHADYRLMSKRALDGLAQFPERNLFLRGMVPLVGYRSTNVYY"
     assembly_gap    287..296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <297..653
                     /locus_tag="ACZPE2_05275"
     CDS             <297..653
                     /locus_tag="ACZPE2_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1319294.1"
                     /translation="NERFAGESKYPLKKMLSFAFDGITSFSISPIRMISAFGAIVCVF
                     AFVMAIYALVQKIIGHTGAGWASLMVSIWFIGGVQLLSVGLIGEYIGKLYKEVKRRPR
                     YIIEAYVNEKEKEDDK"
     gene            653..>957
                     /locus_tag="ACZPE2_05280"
     CDS             653..>957
                     /locus_tag="ACZPE2_05280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319295.1"
                     /translation="MIYDVAIIGGGPAGMSAAIYAARGGMKTLVIEKIAYGGQAIKTY
                     EIDNYPGFANNPTGEELSKAIEEHARKFGATFVSENVKSIENAEYGIKVIHTRRNTY"
     assembly_gap    958..967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <968..1563
                     /locus_tag="ACZPE2_05285"
     CDS             <968..1563
                     /locus_tag="ACZPE2_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319296.1"
                     /translation="KTLGADGEEQFAGAGVSYCATCDGAFFKDKDVCVVGGGNTAMED
                     ALYLAGFCRKVYVLNRSKKFRAAATMVENVKRNGKITMLTDTVVDKFVGSNMLEGVDI
                     RNTVTGEKSILNLSGAIVAIGVKPSSDLAKDCGVECCQHGFVKTDMYLATNITGIFAA
                     GDVRVSPLRQVITAAADGAIAATSAVNYVNELGIKSI"
BASE COUNT          502 a          264 c          398 g          407 t
ORIGIN      
        1 gatatacacg taatacctga tatggtgaga aacttcattg acggatatga cgttgtttac
       61 ggtgtaagaa acaaacgtga taccgataca ttctttaaac gcacaacggc ggtaggattt
      121 tataaattaa tgcagtttat gggcgttaat attgtattca atcacgcgga ttacagattg
      181 atgagcaaac gtgcgcttga cggattggca caattccctg agagaaatct gttcctaaga
      241 ggaatggttc cgcttgtcgg ctatcgctca acgaatgtgt attacgnnnn nnnnnnaacg
      301 aacgctttgc gggcgagtca aaatatccgt taaagaaaat gctttcattt gcgtttgacg
      361 gtataacttc attcagcata agcccaatca gaatgatttc ggcattcggt gcgattgtgt
      421 gcgtgtttgc gttcgttatg gcgatatacg cacttgtgca gaaaattata ggtcacacag
      481 gtgcaggctg ggcgtcactt atggtttcga tatggtttat aggcggtgta cagcttttgt
      541 ctgtcggact tataggcgag tatatcggta aattgtataa agaagttaaa cgccgtccaa
      601 gatatattat tgaagcatat gtaaatgaaa aagagaaaga ggacgataaa taatgatata
      661 tgatgttgca ataatcggcg gaggtcctgc cggaatgtca gctgccatat atgccgcaag
      721 aggcggtatg aaaacgcttg taatcgaaaa aatcgcatac ggcggtcaag caataaaaac
      781 atatgaaata gacaattatc cgggatttgc aaacaatccg acaggtgaag aactttcaaa
      841 agcaatcgaa gaacacgcaa gaaaatttgg cgcaacattt gtttcggaaa atgttaagtc
      901 aatcgaaaat gccgaatacg gcataaaggt tattcacaca agaagaaata cttatatnnn
      961 nnnnnnncaa aaacgctcgg tgcggacggt gaagaacagt ttgccggtgc aggtgtttcg
     1021 tattgtgcga cttgtgacgg tgcgtttttt aaggataagg acgtgtgcgt tgtaggcggc
     1081 ggaaatacgg ctatggagga cgcattgtat cttgcgggat tttgccgtaa agtctatgtc
     1141 ctaaacagaa gcaagaagtt cagagcggcg gctacaatgg ttgaaaacgt aaaaagaaac
     1201 ggcaaaataa ccatgcttac agataccgta gttgataaat tcgtaggctc aaatatgctt
     1261 gagggtgttg atattagaaa cacggtaaca ggagaaaaga gtattttgaa cttatcgggc
     1321 gctatcgtcg caataggcgt aaagccgtcg tccgatcttg caaaagattg cggggttgaa
     1381 tgttgtcagc acggatttgt caagacggat atgtaccttg cgactaacat aacaggtata
     1441 tttgcggcag gtgacgtaag agtatcaccg ttaagacagg ttataacagc ggcggcagac
     1501 ggtgcaatcg ccgcgacaag tgcggttaat tatgtgaatg aactcggaat taaaagcata
     1561 taaaaggtaa aaggataaaa ctatgaagac a
//
LOCUS       DBMDVO010000334         1590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37206_length_1590_cov_1.061115, whole genome shotgun sequence.
ACCESSION   DBMDVO010000334 DBMDVO010000000
VERSION     DBMDVO010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37206_length_1590_cov_1.061115"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="hisF"
                     /locus_tag="ACZPE2_05290"
     CDS             complement(<1..686)
                     /gene="hisF"
                     /locus_tag="ACZPE2_05290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1319297.1"
                     /translation="MYAKRIIPCLDVKNGRVVKGVSFVNLRDAGDPVQCAVEYDKKGA
                     DELVLLDITATNDGRGTMLDIVSRVANSIFIPFTVGGGIRSTDDFKELLRAGADKISV
                     NSAAVRNPDLINDAAYKFGSQCVVCAIDAKRTDNGKWEVYLNGGRIPTGIDAVLWATE
                     AEKRGAGEILLTSMDCDGQKKGYDLELTRAVSESVGIPVIASGGAGELKHFYDAFTVG
                     KADAVLAASL"
     gene            complement(676..1308)
                     /gene="hisH"
                     /locus_tag="ACZPE2_05295"
     CDS             complement(676..1308)
                     /gene="hisH"
                     /locus_tag="ACZPE2_05295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1319298.1"
                     /translation="MIAIIDYGAGNLQSVKKALDFIGAESLVTSRASEIENASACILP
                     GVGSFGDAMNCMTESGLCEAVKKAALGSKPFLGICLGLQLLFDSSEESPGVSGLGILK
                     GRVKRFPSDMGLKIPHIGWNSADIKKPCPIYDGIADGSFVYFVHSYYLDAENRSDVAT
                     KTEYGISFDSSVAVPEINLYATQFHPEKSGRVGLKILENFAKLGGDALVR"
     gene            complement(1455..1539)
                     /locus_tag="ACZPE2_05300"
     tRNA            complement(1455..1539)
                     /locus_tag="ACZPE2_05300"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1503..1505),aa:Tyr,seq:gta)
BASE COUNT          404 a          419 c          356 g          411 t
ORIGIN      
        1 aaaagcgacg ccgccaaaac agcgtccgcc ttgcctaccg taaaagcatc gtaaaaatgt
       61 ttcagctcac ctgctccgcc cgatgcgata acgggaatac cgacgctttc ggagacagct
      121 cttgtaagct caaggtcgta gccctttttt tgaccgtcgc agtccatact cgtcaaaagt
      181 atttcgcctg cgccgcgctt ttccgcctcc gtcgcccaaa ggaccgcgtc aattcccgtg
      241 gggattcgtc cgccgtttaa atagacctcc cattttccgt tgtcggtacg ctttgcatca
      301 atggcgcaga caacgcattg gcttccgaat ttgtatgccg cgtcatttat aaggtcggga
      361 tttctgacag ccgcggaatt caccgaaatt ttatcggcac cggctctgag cagttcctta
      421 aagtcgtcgg tagagcggat tccgccgccc accgtaaacg gaatgaatat agaatttgct
      481 actcgtgaga ctatatcgag cattgtgcct ctgccgtcat tggtagcggt tatatcgaga
      541 agcacaagct cgtctgcgcc ctttttatca tattcaacgg cgcattgcac gggatcgccg
      601 gcgtcgcgta aatttacaaa gctgacaccc ttgacaaccc tgccgttttt aacgtcaaga
      661 cacgggataa ttcttttagc gtacaagcgc gtcacctccg agctttgcaa aattctcaag
      721 tattttaagt cctactctgc cgctcttttc ggggtgaaac tgcgtagcat agaggtttat
      781 ttcggggacc gccacggaag aatcgaacga aattccgtat tctgttttcg tagcgacgtc
      841 cgagcgattt tcggcgtcga gataatagga atgaacaaaa tagacaaacg agccgtctgc
      901 aatgccgtca tatatagggc aaggcttttt gatgtctgcg gaattccacc cgatgtgcgg
      961 aattttaagc cccatatccg acggaaaacg cttcaccctt ccttttaata tgccgagccc
     1021 ggaaacgccg gggctttcct cggaggagtc aaaaagaagc tgcagcccga gacaaattcc
     1081 caaaaacggc ttgcttccga gtgccgcctt tttaacggcc tcacaaagtc cgctttcggt
     1141 catacaattc atggcatcgc cgaacgagcc gacacccggc aaaatgcaag cacttgcatt
     1201 ttcaatttcg gacgctctgc tcgtaacaag gctctcggct ccgatgaaat caagagcttt
     1261 ttttacgctt tgtaaattcc cggcgccgta atctattatt gctatcatat aacgcttctc
     1321 cccgaagaaa aaatatgaaa tattgtacca ttattctttt ttgtttgcaa ccgtcggaga
     1381 cacagcattt ataccattta caaaacccat cggaaaataa aaatcggcaa gtatcaaaag
     1441 atactcaccg attttggtgg gagagagtgg attcgaacca ctgaagtcag tgacgacaga
     1501 tttacagtct gttccctttg gccgctcggg aactctccca tattaaatta tgatgcgtaa
     1561 aatggagctg gtggacggac ttgaaccccc
//
LOCUS       DBMDVO010000335         1587 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37286_length_1587_cov_1.726783, whole genome shotgun sequence.
ACCESSION   DBMDVO010000335 DBMDVO010000000
VERSION     DBMDVO010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37286_length_1587_cov_1.726783"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            27..545
                     /locus_tag="ACZPE2_05305"
     CDS             27..545
                     /locus_tag="ACZPE2_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319299.1"
                     /translation="MNSEELGKRIKEARLAKKMTQSELVGTFITRNMLSRIESGNACP
                     SVKTLEYLAGRLDLPAGSLISDEVQGEEDPDRNAQQLITVKRLYKDSDYSACIKAAEK
                     LIGSEFEDEGQAITARCCIALSEKAMNSGDKAAAIKYAKQALELADKGIYKSREISVD
                     ATLKLSEIAGGK"
     gene            547..825
                     /locus_tag="ACZPE2_05310"
     CDS             547..825
                     /locus_tag="ACZPE2_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319300.1"
                     /translation="MDLIIKMLCDILFDVGTTAGTSHRLPKPLRIICCVPALLAFIAI
                     FAIMAIVAVKCVQDMPAVSILLGALDIIMIFLLLSIFKRAFGTGKDKK"
     gene            1086..1174
                     /locus_tag="ACZPE2_05315"
     tRNA            1086..1174
                     /locus_tag="ACZPE2_05315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:cga)
BASE COUNT          468 a          322 c          383 g          414 t
ORIGIN      
        1 tttatcaatg cggaaggagt gagaatatga attctgaaga actgggaaaa agaattaaag
       61 aagcacgact tgccaagaag atgacacaat ccgagcttgt cggaacgttc attacaagaa
      121 atatgctcag ccgtatcgaa agcggaaacg cctgcccgtc cgtgaaaacg cttgaatatc
      181 tggcaggacg gcttgacctg ccagcaggaa gtcttatatc cgacgaggtg cagggcgagg
      241 aagatcccga cagaaacgca caacagctta taacggtaaa gcgactgtac aaggacagcg
      301 attattccgc ctgcataaag gcggcggaaa agctcatcgg cagtgagttt gaggacgagg
      361 gacaagcgat aacggcacgc tgttgtatag ctttatcgga aaaagctatg aacagcggag
      421 ataaagcggc ggcaataaaa tatgcaaagc aggcgcttga gcttgccgac aagggcattt
      481 acaaaagccg tgaaataagc gttgacgcaa cgctgaagct gtcggaaatc gcaggaggaa
      541 agtaatatgg atctcattat taagatgcta tgcgatatac ttttcgacgt aggaacaaca
      601 gcaggcacaa gtcacagact gccgaaaccg ttaaggataa tctgctgtgt gccggcattg
      661 cttgctttca ttgcaatatt tgcgataatg gctatagttg cggtaaaatg tgtgcaggat
      721 atgcctgctg taagcatact gcttggcgca cttgatataa ttatgatatt tttgcttctg
      781 agcatattca aaagggcttt cggaacagga aaagataaaa aatgattgac aaagctcttt
      841 ttttggtgta taatattatc gttgtgatgt cggaagacgt cacaggatat atgcagacgt
      901 atcgaagtgg tcataacggg gctgactcga aatctccttc ggcaaacgga agcccgactt
      961 ccgcaaagcc tttaaccgtg cgggtttcgg gcattcgccc gccgcaggta caccgtgagt
     1021 tctaacagga attctaatac aaccgaatag ttgcgttaat ctcggttttc cgagttaaca
     1081 tatatggaga cgtatcgaag tggtcataac ggacatgact cgaaatcatg atgccccttg
     1141 agggacgagg gttcgaatcc ctccgtctcc gccaacggaa cccttgaaaa cccagtgttt
     1201 tcaagggctc tttctattcg gcgaaaattc tccgaccttg attttttgct tttcagatgc
     1261 atttttgagc cggttctatg ctcttttctc cccgcgaata ttagaataaa aaatctccca
     1321 ataccgcatg aaattaggct ttttaagtct tgcatatttt ttgcggcgtt agaataattt
     1381 tcgccagaca gaaatttgcc gatttttgaa acacccggtt gcggacaacc gcaccaaaag
     1441 tcatgtgtgg tctttacagg ttagggttca aatcggcaga aagaaaaatg tgctttgctt
     1501 ctttttgttt tgacattata cagaagtgct ccgtttgaag aatctgattg tgtgaaatcc
     1561 tattgcaaaa actcttaata tgtgata
//
LOCUS       DBMDVO010000336         1587 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37294_length_1587_cov_1.253028, whole genome shotgun sequence.
ACCESSION   DBMDVO010000336 DBMDVO010000000
VERSION     DBMDVO010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37294_length_1587_cov_1.253028"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="ACZPE2_05320"
     CDS             complement(<1..480)
                     /locus_tag="ACZPE2_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HIR1319301.1"
                     /translation="MNKSFYKNSTKTAIGGIIAALSIVLMLLTSAIPTLTYAIPAIAG
                     LLLVIIVIEVNKKWAFGVYFVVGVLSMLLVADKEAAVMYVMFFGYYPIIKAMVEKKLN
                     KALSWTVKFIIFNISMILAFLIVTYVFKVPFEEMKKYGAAAAWILLALGNVVFLIYDI
                     "
     gene            complement(484..1125)
                     /locus_tag="ACZPE2_05325"
     CDS             complement(484..1125)
                     /locus_tag="ACZPE2_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR1319302.1"
                     /translation="MNTDPYRVLGVGRNASNDEIKEAYRTLSRKYQSDNYSSSPLSDI
                     AEKKMDELDRAYDAIMAERYGDNAASYGPSYNTSAQHNGYCGADNGYGNSQYPDVRAQ
                     IRSGRIDDAETILDGIPKDIRNAEWYFLKGQVQQRRGWFDEAYKNYSTACSMEPQNRE
                     YTDAFNNLNNNASGGYRTSHEKGTGCSACDICSGLMCADCCCECMGGDLIPWC"
     gene            complement(1126..>1587)
                     /locus_tag="ACZPE2_05330"
     CDS             complement(1126..>1587)
                     /locus_tag="ACZPE2_05330"
                     /inference="COORDINATES: protein motif:HMM:NF039826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR1319303.1"
                     /translation="YPDLCAVIEKTMNEQAQIENGKTLSTDLVADPSAKALAAIMTYG
                     IQNEEKRLISKRVGYCLGRWVYLTDAYDDITKDLKSHNYNPFIEKYKIESKAFDREAI
                     IKSLRLTANEAALAFNLLDIKCYKEILENIIFDGLENQQKMITEKDKEVSR"
BASE COUNT          465 a          358 c          277 g          487 t
ORIGIN      
        1 tatatcatag attaaaaaca caacattgcc gagagccaac aaaatccaag cggctgcggc
       61 accatacttt ttcatctctt caaacggaac cttgaataca taggtgacaa tcaaaaacgc
      121 caatatcatt gagatattaa atattataaa tttaacggtc caagacagtg ctttgttgag
      181 ttttttctca accatagcct taataatcgg atagtagccg aaaaacatca catacataac
      241 ggccgcctct ttatcggcaa caagcaacat cgagagcacg ccgactacga aataaacacc
      301 aaaagcccat tttttgttaa cttcaataac tattataaca agcaaaaggc cggcaatagc
      361 aggtatggca tacgtaagtg tcggtatcgc cgaagtcaaa agcataagaa caattgacaa
      421 agcggcaata ataccgccga ttgcggtttt cgtcgaattt ttataaaagc tcttattcat
      481 agctcagcac catgggatta aatcgccgcc catacattcg caacagcaat cggcacacat
      541 aagtcccgaa caaatgtcac aagcactaca ccctgttcct ttttcatgag aagtacggta
      601 tccgcctgat gcgttattat tcaaattgtt aaaagcatct gtgtattctc gattctgagg
      661 ttccatactg caagcggtgg agtaattttt atatgcttca tcaaaccaac cgcgcctctg
      721 ctgaacctga ccttttaaaa aataccactc ggcatttctg atatccttcg gaataccgtc
      781 aagaatcgtt tcagcatcat caattcttcc ggaccgaatt tgcgctctca catccggata
      841 ctgtgaattt ccatagccgt tatcagcacc gcaataaccg ttgtgttgag cggaagtgtt
      901 gtaagaagga ccataagatg cagcgttgtc tccatagcgt tcggccatga tggcatcgta
      961 tgccctgtca agttcatcca tttttttctc agcaatatcg gagagcggac tgctcgaata
     1021 attgtccgac tgatactttc tggataacgt tcggtatgct tcttttattt catcatttga
     1081 ggcatttctg ccgacaccaa gaactctgta cggatctgta ttcatttatc tgctcacctc
     1141 tttatctttt tcagtaatca ttttttgttg attctcaaga ccatcaaaaa taatgttttc
     1201 aagaatttct ttatagcatt ttatatcgag caaattaaaa gcaagcgcag cttcatttgc
     1261 ggtaagccgc aatgatttga ttatagcttc acgatcaaac gctttgcttt caatcttata
     1321 tttctcaatg aacggattgt aattatggct ctttaaatct tttgtaatat catcgtatgc
     1381 atcggtcaga tatacccatc tgccgaggca ataaccgact cttttcgata tgagtctctt
     1441 ttcttcgttt tgtatgccgt aagtcattat agcagccaaa gcctttgcac ttggatcggc
     1501 cacaagatct gttgataagg tctttccatt ttcaatttga gcttgttcgt tcatagtttt
     1561 ttctataacg gcacacaaat caggata
//
LOCUS       DBMDVO010000337         1583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37439_length_1583_cov_1.154521, whole genome shotgun sequence.
ACCESSION   DBMDVO010000337 DBMDVO010000000
VERSION     DBMDVO010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37439_length_1583_cov_1.154521"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="pheS"
                     /locus_tag="ACZPE2_05335"
     CDS             <1..753
                     /gene="pheS"
                     /locus_tag="ACZPE2_05335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1319304.1"
                     /translation="KETIDVTLPGKKHEIGHKHPLSIVLDEVKDIFVGMGFQIATGPE
                     VEWDYYNFEALNLPPDHPARDTQDTFYITEKMLLRTQTSPVQIRVMEKQEPPIRIIAP
                     GRVYRSDAVDATHSPFFHQIEGLVVDKGITMGDLKGSLMQFAKRLYGEDTEIRLRPHH
                     FPFTEPSCEMDVTCFKCGGKGCPMCKGEGWVEILGGGMVHPKVLKNGGVDPEVYSGYA
                     FGVGLERLTMFRFNIDDMRLLFENDARFLNQF"
     gene            769..>1583
                     /gene="ytpR"
                     /locus_tag="ACZPE2_05340"
     CDS             769..>1583
                     /gene="ytpR"
                     /locus_tag="ACZPE2_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIR1319305.1"
                     /translation="MNLSKRWLHDYVNLDVSDQQFADDMTLSGSKVESFETEGADIKN
                     VIVGKVVSLEKHPDSDHLWICQIDVGAKDNIQIVTGAQNLKVNDVVPVAMDDSFVSGG
                     HHIKKGKLRGVESNGMLCSLGELGLTIHDFPYAIEDGIFVLGDDCDKTLGKDIHEAIG
                     LDDVITEFEITSNRADCLSITGLAREAAATFGSKLVIPEPAVKKTHGDVNDFLSVEIK
                     EPSLCYRYAGAVVENVRVKPSPRWMRERLRACGVRPINNIVDITNFVMLEYGQP"
BASE COUNT          427 a          351 c          409 g          396 t
ORIGIN      
        1 aaagaaacga tagatgttac gcttcccggc aaaaagcatg aaataggtca taagcatccg
       61 ctctcaatag ttcttgacga ggttaaggat atatttgtcg gtatgggctt ccagatagct
      121 actggccccg aggttgagtg ggattactac aatttcgagg cgcttaatct tccgcccgat
      181 catccggcaa gagatacgca ggatactttc tatatcacgg aaaaaatgct tcttcgtaca
      241 cagacttctc ccgtgcagat tcgtgtaatg gaaaagcaag agccgcctat aagaattatt
      301 gcacccggca gagtttaccg ctctgatgcg gttgacgcaa cacattcgcc gttcttccat
      361 caaatcgagg gattggttgt ggataaagga attacaatgg gcgatctcaa aggcagtctt
      421 atgcagtttg caaagagact ctacggcgag gataccgaga taagacttcg tccgcaccac
      481 ttcccgttta ccgagccgtc ttgcgaaatg gacgttacat gctttaagtg cggcggcaag
      541 ggctgcccca tgtgtaaggg cgagggctgg gtagaaatac tcggcggcgg tatggttcac
      601 ccgaaggtgc ttaaaaacgg cggagtagac ccggaggttt attccggcta tgctttcggc
      661 gtagggcttg aaagacttac aatgttccgc tttaatatcg acgatatgcg tctgctcttt
      721 gagaacgacg ctcgtttctt aaatcagttt taaggggagg gttatataat gaatttatct
      781 aaaagatggc ttcacgacta tgtgaattta gatgtgtcgg atcagcagtt tgccgatgat
      841 atgactcttt ccggctccaa ggttgagtcc tttgagaccg agggtgccga catcaaaaac
      901 gtaattgtag gtaaggttgt atcacttgaa aaacatcccg attccgacca cctttggatt
      961 tgccaaatcg acgtcggagc aaaggataat atacagatag ttaccggcgc tcagaattta
     1021 aaggtaaacg atgttgttcc cgtagcaatg gatgatagct tcgtcagcgg cgggcatcat
     1081 attaaaaagg gcaagctccg cggagtggag tcaaacggta tgctctgctc actcggcgag
     1141 ctcggactta caatacatga tttcccgtat gcgattgagg acggtatttt cgttctcggc
     1201 gatgattgcg acaaaactct cggcaaggat atacacgagg caataggact tgacgacgtt
     1261 atcaccgaat ttgaaataac gtcaaacaga gccgactgcc tttcgataac cgggcttgca
     1321 agagaagccg cggcaacctt tggctcaaag cttgttattc cggagcccgc ggtcaaaaag
     1381 actcacggcg acgtaaacga ctttttgtcg gttgaaataa aggagccgtc gctctgttac
     1441 agatatgccg gcgcggtagt tgaaaatgtc cgcgttaagc cgtcgccgcg ttggatgcgc
     1501 gagagacttc gcgcatgcgg agtaagaccc ataaacaata tagttgacat tacaaacttt
     1561 gtaatgctcg aatacggcca gcc
//
LOCUS       DBMDVO010000338         1578 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37584_length_1578_cov_1.462424, whole genome shotgun sequence.
ACCESSION   DBMDVO010000338 DBMDVO010000000
VERSION     DBMDVO010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37584_length_1578_cov_1.462424"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="ACZPE2_05345"
     CDS             <1..185
                     /locus_tag="ACZPE2_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319306.1"
                     /translation="LTALPEVLRGLRDYRMLIYAVVLIVMMIMTSSPKAIAFWETHSI
                     KKFFKKGNGKTKKEAR"
     gene            188..946
                     /locus_tag="ACZPE2_05350"
     CDS             188..946
                     /locus_tag="ACZPE2_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319307.1"
                     /translation="MALLEAKNLSITFGGLKAVDDFNIEIEKGELYGLIGPNGAGKTT
                     IFNLLTGVYKPTEGQATLDGTVITGKKTIDICKAGVARTFQNIRLFGQMSVLDNVKVG
                     LHNHYKYNTAAGIFKLPSYFKKERAMDEKAMELLKVFDLDKEASYLSSNLPYGKQRKL
                     EIARALATEPKLLLLDEPAAGMNPNETEELMETIRFVRDEFDMTILLIEHDMKLVSGI
                     CEELTVLNFGHVLCQGKTSDVLNNPEVITAYLGE"
     gene            959..>1578
                     /locus_tag="ACZPE2_05355"
     CDS             959..>1578
                     /locus_tag="ACZPE2_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319308.1"
                     /translation="MALLEIKDLEVNYGVIKAIKGVSFDVNEGEIIALIGANGAGKTT
                     ILHTITGLIQAKKGSIVFDGKELTKTPPHKIVSMGMAHVPEGRRIFQQLSVLENLKLG
                     AYTRKDKSEIASTLKMVYERFPRLEERKNQIAGTLSGGEQQMLAMGRALMSKPRIILM
                     DEPSMGLSPLLVSEIFDIIKVINESGTTVLLVEQNAKKALSIADRA"
BASE COUNT          461 a          331 c          381 g          405 t
ORIGIN      
        1 tgcttacggc tttgccggag gttctccgcg gacttcgcga ttacagaatg ctcatatatg
       61 cggtagtgct tattgttatg atgataatga cctcaagccc caaggcaatc gctttctggg
      121 agactcattc gataaaaaag ttcttcaaaa agggaaacgg taagaccaaa aaggaggcac
      181 ggtaattatg gcattgctcg aagctaaaaa tttaagcata accttcggcg gtcttaaagc
      241 cgttgatgat tttaacatag aaatagaaaa aggcgagctc tacggtctta taggaccgaa
      301 cggcgcaggt aaaacgacca tattcaacct tcttacgggc gtatataagc ctaccgaggg
      361 tcaggctact cttgacggca ccgttataac gggcaaaaag accattgata tatgcaaggc
      421 aggcgttgca agaaccttcc aaaatatccg tcttttcggt cagatgtcgg ttcttgataa
      481 tgttaaggta ggacttcaca atcattataa gtacaatacg gcggcaggta tattcaagct
      541 tccgtcatat ttcaaaaaag agcgtgcaat ggacgaaaag gccatggagc ttttaaaggt
      601 tttcgacctt gataaagaag cgtcttatct ttcttcaaat cttccgtacg gaaagcagcg
      661 caagcttgaa atagcgagag cacttgcgac tgagccgaag cttttgctcc ttgacgagcc
      721 ggcggcggga atgaatccca atgagacaga ggagcttatg gagacaatac gctttgtccg
      781 cgatgagttc gatatgacca tacttcttat agagcacgat atgaagcttg tttccggcat
      841 atgcgaggag cttacggttc ttaacttcgg tcatgtactg tgtcagggca aaacgtccga
      901 tgtgctcaat aatcccgaag ttatcacagc atatctcggc gaataaggag gagcagctat
      961 ggcattactt gaaattaagg atcttgaagt taattacggc gttataaagg ctattaaggg
     1021 cgtttccttt gatgtaaacg agggcgaaat tattgcactt atcggagcca acggcgcagg
     1081 taaaacaacc attcttcaca cgattaccgg ccttattcag gctaaaaaag gctctattgt
     1141 gtttgacggc aaagagctta ctaaaactcc gccgcacaaa atagtttcca tgggtatggc
     1201 acacgttccg gagggcagac gtattttcca gcagctttcg gtactcgaaa acctaaagct
     1261 cggcgcatat acccgcaagg ataaatccga aattgcctca actcttaaaa tggtttacga
     1321 gcgttttccg cgacttgagg aacgtaaaaa tcagattgca ggcacgttgt cgggcggtga
     1381 gcagcagatg cttgcaatgg gcagagcact tatgtccaag ccgagaatta ttttgatgga
     1441 tgaaccgtcc atgggacttt ccccgttgct tgtttcggaa atctttgata taataaaggt
     1501 gatcaatgag agcggaacaa ccgttctttt agttgagcaa aacgccaaga aggctctttc
     1561 gattgccgat agggcata
//
LOCUS       DBMDVO010000339         1578 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37602_length_1578_cov_1.017603, whole genome shotgun sequence.
ACCESSION   DBMDVO010000339 DBMDVO010000000
VERSION     DBMDVO010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37602_length_1578_cov_1.017603"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..1276
                     /locus_tag="ACZPE2_05360"
     CDS             182..1276
                     /locus_tag="ACZPE2_05360"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1319309.1"
                     /translation="MILYGSLALTGRGHGTDRIVKETLSPIDTTVEFDFEKTDLPHPN
                     TMELFAYKDDKLCDSMLACSIGGGEVTIKGMKMAESKPIYEFSTFKDIAEHCRKNDIR
                     IWEYVEKTEGSDIWDFLGEVWDCMRDCIKDGLNTEGILPGGLGVSRKAGFLFRQNHID
                     ESPETRENRIVCAYAYAVGEQNAAGGRIVTAPTCGASGVLPAVMLYFQKKRGYSGREI
                     EQALATAAIIGLLVKTNASISGAECGCQAEIGTACAMTAAALGELFGMSLEQIEYAAE
                     NAIEHHLGLTCDPIYGLVQIPCIERNAVAAMRSINAINLANFLTATRKISLDLIIETM
                     YETGRDLSAKYRETSTGGMAKLYHPNIKCD"
BASE COUNT          432 a          357 c          396 g          393 t
ORIGIN      
        1 gctttacttt ttcttcaaac gcacccgccg gtaccgtcag ggtaagtaca gcggaatctg
       61 tattaaccgt gtagaccgca tcatccacac tgccggaaag tcccagctct gcagcggtcg
      121 tcacgaatgc agcagctgcc gtatttttaa gaagaaaaac cctgatgtcg ataaattcaa
      181 ggtgatatta tacggctccc ttgccttaac aggcagaggt cacggcaccg accgcatagt
      241 caaggaaaca ttgtctccga ttgacacaac cgttgaattt gattttgaaa aaaccgacct
      301 tccgcatcca aatactatgg agctttttgc ctataaggat gataagcttt gcgacagtat
      361 gctcgcctgc agcataggtg gcggcgaagt cacaatcaag ggtatgaaaa tggctgagag
      421 caagccgata tacgaattca gcacatttaa ggatattgcg gagcattgcc gcaaaaacga
      481 tatacgtata tgggagtatg tcgaaaagac cgaggggagc gatatatggg attttctcgg
      541 cgaagtctgg gattgtatgc gtgattgcat aaaagatgga cttaatacag agggaatttt
      601 gccgggagga ctcggcgtgt cgagaaaagc gggattttta ttcaggcaga accatattga
      661 cgaatctccc gaaactcgtg aaaaccgcat tgtttgcgcg tatgcttacg ctgtgggtga
      721 acaaaatgcc gcgggcggcc gcatagttac cgcgcccacg tgcggtgcaa gcggagtctt
      781 gcctgccgta atgctatatt tccaaaaaaa gaggggatac agcggcagag aaatagaaca
      841 agcactcgct acggcggcga ttatcgggct tttggtaaaa acaaatgcat cgataagcgg
      901 tgcagagtgc ggctgtcagg cagaaatcgg aacggcgtgt gccatgaccg cggctgctct
      961 cggcgagctt ttcggaatga gccttgagca gatagagtat gccgccgaaa acgccataga
     1021 gcatcatttg ggacttacgt gcgacccgat atacggcctc gtgcaaattc cgtgtataga
     1081 gcggaatgcc gtagccgcaa tgcgttccat aaatgccatt aaccttgcca atttccttac
     1141 cgcaacgcga aagatctcgc ttgacctcat aatagaaacg atgtatgaaa caggcagaga
     1201 cctctccgct aagtaccgcg aaacaagcac cggcggtatg gctaagcttt atcatcccaa
     1261 cataaagtgc gattaacggt attttatgat gcggcggttg cctgcactta ttattgtttc
     1321 ggcgcggcac agcagtacca agattgatta agcctgccgt tttcctcttt gatttcacgg
     1381 ctgccgttgt cctatttaat cttgcaattt agacaatttg gattttttaa aattaccgta
     1441 aaaaaatgtt gactttgtct gtgcctcata gtataatatc attcgcaacg aaaaagaagt
     1501 tgtcgctatt ggggaattgt gtaacggtag cacgacagac tctgactctg tttgttgggg
     1561 ttcgaatccc tattcccc
//
LOCUS       DBMDVO010000340         1576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37653_length_1576_cov_0.793898, whole genome shotgun sequence.
ACCESSION   DBMDVO010000340 DBMDVO010000000
VERSION     DBMDVO010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37653_length_1576_cov_0.793898"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="ACZPE2_05365"
     CDS             complement(<1..373)
                     /locus_tag="ACZPE2_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319310.1"
                     /translation="MSFFIKDTEIKIGFLFAASVAALLCFDVGPQVQLSVLSGILHEG
                     GHVFSLLLLGERPQKICFGLFGLTVLRRNDIKLNYKNEIITAFCGPFVDIAICVCALL
                     FYLNYRDNQALFSVVAINAALA"
     gene            complement(373..966)
                     /locus_tag="ACZPE2_05370"
     CDS             complement(373..966)
                     /locus_tag="ACZPE2_05370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1319311.1"
                     /translation="MKSIKKSADFVFEPINDTDKVKKNDETESYSQEAFSLASYDDNE
                     LTGIGGEDILTAKEKTSFAPVSMSQKAVMPVENAKITSKFGYRVNPVTEEYGFHTGLD
                     LAVREGTPIRATFGGTVTKAQESKLRGNYVVISHSDNVETVYCHCKELFVSEGDIVSA
                     GDIIALAGMTGQATGPHLHFELKINGLWCNPVWILDI"
BASE COUNT          524 a          334 c          286 g          432 t
ORIGIN      
        1 aagcaagtgc agcatttata gcaacaaccg aaaataaagc ttgattatct ctgtaattaa
       61 gatagaacaa cagtgcacat acacaaatcg ctatatccac aaaaggtccg caaaaagcgg
      121 taattatttc gtttttataa tttagcttaa tatcgttacg acgcaaaacg gtgagaccga
      181 aaaggccgaa acagattttc tgcggacgtt ctcctaaaag caaaagtgaa aaaacatgtc
      241 cgccctcgtg gagaattccc gaaaggacgg acaattgcac ctgcggtccg acatcaaaac
      301 acaaaagtgc tgcaacagat gccgcgaaca aaaagccgat tttaatttcg gtatctttaa
      361 taaaaaaaga catcagatat caagtatcca aacagggtta caccacaacc cgtttatttt
      421 gagttcgaaa tggagatgcg gtcctgtagc ctgcccggtc atgcctgcaa gcgcaatgat
      481 gtcacctgcc gaaacgatgt caccttccga aacaaaaagc tctttgcagt ggcaataaac
      541 agtttcgaca ttatctgaat gagaaatgac aacatagttg ccgcgaagct tagattcctg
      601 tgcttttgtg acggttccgc cgaaggtcgc tcttataggc gtaccctccc gcaccgccaa
      661 atcgagtccg gtgtgaaagc catattcctc cgtaacagga tttacacgat agccgaattt
      721 tgatgtgatt tttgcatttt ctaccggcat tacagctttt tgagacatac ttaccggcgc
      781 aaatgatgtt ttttcttttg cggtcaatat atcctctccg ccgattccgg taagctcgtt
      841 atcatcatac gaagcaagtg agaaagcttc ttggctatac gattcggttt cgtcgttttt
      901 tttgaccttg tcggtatcgt ttataggctc aaaaacaaaa tcggcagatt ttttaatgct
      961 tttcacaacc tccgcaaacg gaatatcggt agatgaaata ttataatagg actcacgaag
     1021 aaataaaaag acagacgatt gagatttaaa cattataatc ataaaaattc ctattgcaat
     1081 acagagtata atttgcactt tcaaaacagg cagcaagaag tttgttttca ctttgggttc
     1141 cccggactcg tgtcctcttc ggtattcaca ataatcgtat tttttgccgg aggcgatacc
     1201 gttaggctca tgctcgtaag cgttatacat ttaaatcacc cgataatgta tattaaagca
     1261 aaaaaagaaa aatgcctttt cattttgaaa agacatttaa agcaaatttc atttattata
     1321 acacccgatt caaaggtgcg gtgatttgct atataatcaa tcgtcccgaa aacaagcatt
     1381 aaagacacca caaaagcaag tactatcaaa aatattgctt tgaacttgtt ttttggcccc
     1441 ataggctact cctcatatac aaaatattaa gaaaataata ccatttcaaa taatcacagt
     1501 caacacaaat caggcaaaaa aataacaccc ggctaaagcg gatgctataa aaacggtact
     1561 tttgcttgcg aacaaa
//
LOCUS       DBMDVO010000341         1574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37717_length_1574_cov_1.231500, whole genome shotgun sequence.
ACCESSION   DBMDVO010000341 DBMDVO010000000
VERSION     DBMDVO010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37717_length_1574_cov_1.231500"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="ACZPE2_05375"
     CDS             complement(<1..881)
                     /locus_tag="ACZPE2_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR1319312.1"
                     /translation="MQQSVKARFKTVLHRTYRKFSHQSLFTKFLSVFVAMVLICFLLL
                     GVTLGVFLSKYWSSQKEQLLSSNAKNIANTTSSVLSSRIQTDASGSIAMICSNLSMIS
                     DAISADIYITDTSGNIILCKDVITQNYSISNNGRCSLHGGYTIPQEVIDEAKKGDYFA
                     KTTLNGVYMKKQLVAAEPVKVGEETAAVVFAVAPMTAGFTNYAMNILKMFIFACLMAF
                     SVGFIAVYFLTASLIKPLHDMSEATKAYAEGDFGPRVKVKGDDELAELVRAFNKMASD
                     LSLLESSRRSFVANVSH"
     gene            complement(887..1564)
                     /locus_tag="ACZPE2_05380"
     CDS             complement(887..1564)
                     /locus_tag="ACZPE2_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1319313.1"
                     /translation="MLVVDDDTNICELLRLYLEKEGYVVKIVNDGVSAINAFKQENPD
                     LTLLDIMIPKLDGWQVCREIRKFSDKPIIMLTAKGETFDKVLGLELGADDYVTKPFDT
                     KEVVARIKAVLRRTAPASDTSDVKEVNYDKLSINLTNYEMKVNGVSVDTPPKELELIY
                     HLASNPNRVFTRDQLLDEVWGFDYYGDSRTVDVHIKRLREKLEGVSDKWELKTVWSVG
                     YKFETKD"
BASE COUNT          370 a          370 c          378 g          456 t
ORIGIN      
        1 tcgtgcgaga cgtttgccac aaaacttcgc cgcgagcttt caagaagcga cagatccgat
       61 gccatcttat tgaatgcgcg cacaagctcg gcgagttcgt cgtcaccctt taccttgact
      121 ctcgggccga aatcgccctc tgcgtaagct tttgtcgcct ccgacatatc gtggagtggt
      181 tttataagag aagcggtgag gaaatatacc gcgataaagc ctaccgagaa agccataaga
      241 caggcgaaaa tgaacatttt aaggatattc atcgcatagt ttgtgaagcc cgccgtcata
      301 ggcgccacgg caaaaacaac tgctgcggtt tcttcgccga cttttaccgg ttccgccgcg
      361 acaagctgct ttttcatata gacgccgttt aaggtggtct ttgcaaaata gtcgcccttt
      421 ttcgcctcgt cgattacctc ctgaggtatt gtgtagccgc cgtgcagact gcatctgccg
      481 ttgtttgaga tactgtaatt ctgtgttatg acgtccttgc acaatattat gttgcccgag
      541 gtatcggtta tgtaaatatc agccgagata gcgtccgaaa tcatcgagag gttactgcat
      601 atcatcgcta tcgagccgga ggcatcggtt tgaattcttg atgagagaac agatgaggta
      661 gtgttcgcta tgttttttgc gttagaggag agaagctgtt ctttttgact tgaccagtat
      721 tttgagagga acacgccgag cgttactccc aaaagcaaaa agcatatgag taccattgcc
      781 acaaataccg acagaaattt agtgaacagt gactgatgac tgaatttgcg gtaggtgcgg
      841 tgcaataccg ttttaaaacg cgcctttacg ctctgctgca ttcttatcag tccttagttt
      901 caaatttata gcctacgctc caaacggttt tcaattccca tttgtcggaa acgccctcaa
      961 gcttttcgcg cagacgcttt atgtgaacgt ctaccgttct tgagtcgccg tagtagtcaa
     1021 acccccatac ctcgtcaaga agctggtcgc gcgtaaatac acggttggga tttgaggcga
     1081 gatgatatat aagctcgagc tctttcggag gagtgtctac ggatactccg ttgaccttca
     1141 tctcgtaatt tgtaaggttt atcgagagct tgtcgtaatt tacctctttg acgtcgcttg
     1201 tgtcggaagc gggagcggtg cggcgaagta cggcttttat tcttgccaca acctcttttg
     1261 tgtcaaatgg ctttgtaaca tagtcgtccg cgccgagctc aagacccaga accttgtcaa
     1321 aggtctcgcc cttggcggtg agcataatta tcggcttgtc cgagaatttt ctgatttcac
     1381 ggcatacctg ccagccgtca agcttcggta tcattatgtc gagcagcgtg aggtcgggat
     1441 tttcctgctt gaatgcattt attgccgaca caccgtcgtt aactattttt acgacgtagc
     1501 cctctttttc gagatagagc cttaaaagct cgcagatatt ggtgtcgtca tcgacgacga
     1561 gtattttttt gttt
//
LOCUS       DBMDVO010000342         1574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37730_length_1574_cov_0.773252, whole genome shotgun sequence.
ACCESSION   DBMDVO010000342 DBMDVO010000000
VERSION     DBMDVO010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37730_length_1574_cov_0.773252"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="ACZPE2_05385"
     CDS             <1..774
                     /locus_tag="ACZPE2_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1319314.1"
                     /translation="LPRIDGAAALFPVYSTFVNAVYPNTTKLHDGIFEYNNTPDGYRL
                     LAEKETDIFIGVYPSDEQRAYAEENQTTFEYTPIGTEAFVFFVHKDNPITNLTTKQIK
                     DIYSGKITNWKDVGGKNEKIAAFQRNEGSGSQSMLKRFMGDTPITDAPTEMVNDLMAG
                     IIEQVADYKSKTNSIGFSFRYYVEGIIKNPDIKMISIDGAAPTSENIRNGSYPILTPI
                     YAVTYKENQNGNVDKLLEWILSDEGQYIINETGYVGIKN"
     gene            787..>1574
                     /locus_tag="ACZPE2_05390"
     CDS             787..>1574
                     /locus_tag="ACZPE2_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR1319315.1"
                     /translation="MKKLLSIILTITIICSAAIAAFAEQAYSDEKCSEWAYYYIKDAE
                     SPLYNIMPQEMYGGDFTASVTRQEFCELIYNTVGYMNGVTNVSDEIGKSTPIYPKFSG
                     KTSPFEDTNSEYILAMYESGIIKGKTEKTFCPSDYLTREETALIISRAAQYSNLKKLP
                     YKLPFNDTHSLTDESSDAIKSVCGMGIMNGTGENHFSPSETLSKEQAAAIAMRLINCI
                     AHHSEINTELSCYFNLMQMWIEDKDGNVIFILPMYWATYDYRID"
BASE COUNT          528 a          300 c          334 g          412 t
ORIGIN      
        1 ctgccgagaa ttgacggagc tgccgcattg ttccccgtgt attccacatt tgtgaacgct
       61 gtatatccga atacgacaaa gctgcacgac ggaatatttg agtataacaa tacccctgac
      121 ggctatcgcc ttttggcgga aaaagaaaca gatattttca tcggtgtgta tccgtccgac
      181 gaacaaaggg catacgcaga agaaaaccaa accacctttg aatatacgcc tatcggaacg
      241 gaggcatttg ttttctttgt tcacaaggac aatcctatta caaatcttac caccaaacaa
      301 ataaaagata tttattccgg caagattaca aattggaagg atgtcggcgg aaaaaatgag
      361 aaaattgccg catttcagcg gaatgagggc agtgggagtc agagtatgct gaaacggttt
      421 atgggtgata cgccgattac ggatgcccct accgaaatgg tgaatgattt aatggcgggc
      481 attatagaac aggtggcgga ttataaaagc aaaacaaact caatcgggtt ttcattcaga
      541 tattatgttg agggaattat aaagaatcct gatataaaaa tgatctcgat tgacggtgct
      601 gcaccaactt ccgaaaatat cagaaacggc agttatccca ttcttacacc gatttatgct
      661 gttacctaca aggaaaatca aaacggcaat gttgacaaat tacttgaatg gattttatcc
      721 gatgaggggc aatacattat aaacgaaaca ggttatgtgg gaataaagaa ttaggagggc
      781 aacacaatga aaaaactgtt atcaattatt ttaacaatta caattatttg ctctgcggca
      841 atagctgcat ttgcagagca agcatactct gatgaaaaat gttccgaatg ggcatattac
      901 tacattaaag acgcagaatc ccctctgtat aacataatgc cgcaggaaat gtacggcggc
      961 gattttacag cgtctgttac acggcaagaa ttttgtgagc ttatatacaa tacggtcggt
     1021 tatatgaacg gtgttacaaa tgtttcggat gaaatcggaa aatcaacgcc gatttacccc
     1081 aaattttcgg gaaagacatc accgtttgaa gatacaaaca gcgagtatat tttagctatg
     1141 tatgaaagcg gaataattaa gggcaaaacc gaaaaaacct tttgcccgtc agattatctg
     1201 acaagagagg aaacagctct cattataagc cgcgctgccc aatattccaa tttgaaaaag
     1261 ctgccgtata aattgccttt taatgatact cattctctga cagatgaatc atcggacgca
     1321 ataaaatccg tctgcggaat gggaattatg aacggaacgg gcgaaaatca tttttcacct
     1381 tcagaaacgc tttcaaagga acaggcggct gcaatcgcaa tgcggcttat aaattgcatt
     1441 gcacatcaca gcgaaatcaa cacggaacta tcctgttatt ttaatttaat gcagatgtgg
     1501 attgaggaca aagacggcaa tgtgattttt atacttccga tgtattgggc aacatatgat
     1561 taccgcattg atta
//
LOCUS       DBMDVO010000343         1571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_37809_length_1571_cov_1.195918, whole genome shotgun sequence.
ACCESSION   DBMDVO010000343 DBMDVO010000000
VERSION     DBMDVO010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37809_length_1571_cov_1.195918"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="ACZPE2_05395"
     CDS             <1..950
                     /locus_tag="ACZPE2_05395"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR1319316.1"
                     /translation="GELLSDPTYYGQIVAQTYPLVGNRGIEKRENGSKIMASGYVVRE
                     WCDTGDLGEETLDSYLKECGIVGISGIDTRRLTRAVRDKGYMKGAITDNLNDFDVLIK
                     NIKNYTISGAVEAVTVKEPQKFTAENPKCNVAVIDYGSPRRTLDGFLSRGCSLTVVPA
                     DFTAEQLKNISPDGIFLSDGPADPDDNPKLIENIKEFKKLGVPMFGIGLGHQMLALSE
                     DFKIEKMPQGHRGSNQPVVIKPTGKLMVTEQNHGYSVEVSSISADKADITMINVNDGS
                     CEGLEYKNFNGFGVQFTPDSDRDSSTSWIFDRFVDMMGE"
     gene            954..>1571
                     /locus_tag="ACZPE2_05400"
     CDS             954..>1571
                     /locus_tag="ACZPE2_05400"
                     /inference="COORDINATES: protein motif:HMM:NF052054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIR1319317.1"
                     /translation="MTENKYSKILVLGAGPVVIGSSSEYDYSAVSVCRALKEEGISVV
                     SVNSNPDTIMTDKNVADTVYIEPLNGETVKKIIEKESPDAIIGTTGGEAGLEICLELL
                     QNGYLDEHGTKLLGIKPETIKAIRNEQALRDTLENVGEPYVSAKVLPTDAECVTFAGE
                     IGFPVLISPAFTPDFSEPIRCENADDILSGFGSCAERSLVNEVYVR"
BASE COUNT          461 a          321 c          397 g          392 t
ORIGIN      
        1 acggagagct gctttcagac ccgacatatt acggtcagat agtggcgcaa acctatcctc
       61 ttgtaggcaa cagaggcata gaaaaaagag aaaacggttc aaaaataatg gcaagcggct
      121 atgttgtgcg tgaatggtgc gacaccggcg atctcggcga agaaacgctt gacagctatt
      181 taaaagagtg cggcatagta ggaatcagcg gtattgatac acgccgtctt acgcgcgccg
      241 tccgcgataa aggctatatg aagggcgcta taaccgacaa tctcaatgat tttgacgtgc
      301 ttattaaaaa tatcaagaat tatacaattt ccggggcggt tgaggctgtt accgtaaaag
      361 agccgcagaa attcacggcg gaaaatccga aatgcaatgt tgcggtgatt gattacggtt
      421 caccgcgcag aactcttgac ggctttttgt cacgaggctg tagtttgacc gtcgtcccgg
      481 ctgactttac ggctgagcag ttaaagaaca tttcaccgga cggtattttc ctttccgacg
      541 gtcctgccga tcctgacgat aaccccaagc ttatagaaaa tatcaaggag tttaaaaaac
      601 tcggtgttcc gatgttcgga ataggtctcg gccatcagat gctggctctt tcggaagact
      661 ttaaaattga aaaaatgccg caagggcacc gcggttcaaa tcagcctgta gttataaaac
      721 ctaccggaaa gcttatggtt acagagcaga atcacggcta ttcggttgaa gtttcgagca
      781 tcagtgccga taaagccgat attacaatga taaatgtcaa cgacggctcc tgtgagggac
      841 ttgagtacaa aaatttcaac ggtttcgggg tccagtttac accggacagc gaccgcgaca
      901 gcagtacatc ctggatattt gaccgctttg ttgatatgat gggggaatga gttatgacag
      961 aaaataaata ttcaaaaata cttgttctcg gagccggccc tgtggttata ggctcttcct
     1021 ctgaatatga ttattcggct gtatctgttt gcagagcgtt gaaggaagag ggcatttcag
     1081 tagtttcggt aaactcaaat ccggatacaa taatgacaga taaaaatgtt gcggatacag
     1141 tttatattga gccattaaac ggcgaaacag taaagaaaat aattgaaaaa gaaagtcccg
     1201 atgccattat cggcaccacg ggaggagaag cggggctcga aatttgcctt gagcttttac
     1261 aaaacggtta tcttgatgag cacggtacca agcttctcgg aattaagccg gaaaccataa
     1321 aggcaataag aaacgagcag gctcttcgag atactcttga aaatgtgggc gaaccgtatg
     1381 tatccgcaaa ggtgttgccg acagatgcgg aatgtgtgac attcgccggt gaaatcggtt
     1441 ttcccgtact tataagtccg gcattcacgc ctgatttttc agagccgata cgttgcgaaa
     1501 atgcagatga tattttgtca ggcttcggca gttgtgccga gcggtctctt gtaaacgagg
     1561 tatacgtaag a
//
LOCUS       DBMDVO010000344         1565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38009_length_1565_cov_1.209699, whole genome shotgun sequence.
ACCESSION   DBMDVO010000344 DBMDVO010000000
VERSION     DBMDVO010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38009_length_1565_cov_1.209699"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="ACZPE2_05405"
     CDS             complement(<1..1149)
                     /locus_tag="ACZPE2_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1319318.1"
                     /translation="MSITNYLTFAGGLGLLLYGIKLMGEGLELAAGSRLKHLLEKMTK
                     NRFLAVLVGFLITTLIQSSSATTVMVVGFVNTGLMNLTQAIGVIMGANIGTTTTSVLV
                     SLNLTGIAPIAIFIGAFMILFAKKNSLKYVGQALAGFGMLFMGMELMQNAMDPLKDSE
                     VFRNWIINADNPLVGILIGAGVTSVIQSSAASVGILQALASQGLVPLSFAVYIIFGQN
                     IGTCVTALLSTIGTKTNSKRTAVMHILFNVIGTVVFVVISMFLPFTKWIQAMSDNVMM
                     QISLVHIIFNVVSTIIMFPFAGALVKLSCILVPDKKGKEDDGMHLQFIDERMLNTPPL
                     AVVQVGKEVLRMAALARRNFDIASSDLINKEIKHFDEVNHTEKIINYLN"
     gene            complement(1171..>1565)
                     /locus_tag="ACZPE2_05410"
     CDS             complement(1171..>1565)
                     /locus_tag="ACZPE2_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012931.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR1319319.1"
                     /translation="YFDCKYSVISLKKVYKYNTVKSGFGKSGQRVIGIEAEMWTEWVD
                     SERALEFSVYPRILALSEVAWTNLENRRYKDFLRRLEWYKTYLRKKGVNYSRVTGKSG
                     ISVSKTIYHMGEDGKEFKLSEKRREAEN"
BASE COUNT          443 a          371 c          315 g          436 t
ORIGIN      
        1 attgagataa ttgataatct tttcggtgtg atttacttcg tcaaaatgct taatttcctt
       61 gtttataagg tcgcttgacg caatatcgaa attgcgtctt gccaaagcag ccatacgaag
      121 aacctctttt cccacctgca ctacggcaag cggaggggtg ttgagcattc gctcgtcaat
      181 aaactgaagg tgcattccgt cgtcctcttt accctttttg tcggggacaa gaatgcagga
      241 aagctttaca agggcgccgg caaacgggaa cattattatg gtgctcacta cgttaaatat
      301 gatatgaacg agcgatatct gcatcatcac gttgtcactc atagcctgta tccactttgt
      361 aaacggaagg aacatagata tgacaacaaa aacaaccgtg ccgattacat taaagagaat
      421 gtgcataacc gcggtacgtt ttgaatttgt ctttgtaccg attgtcgaaa gaagcgcggt
      481 aacacaggta cctatattct gaccgaaaat aatatatacc gcgaacgaaa gcggcacaag
      541 cccctgtgat gcgagagcct gaagtatgcc tacgcttgcc gcagagctct gtataacgct
      601 cgtaactccg gcgcctatga gtatgcctac aagaggattg tccgcattta taatccagtt
      661 gcggaaaacc tcggaatcct taagcggatc catggcgttt tgcataagct ccatacccat
      721 aaacagcatg ccgaacccgg cgagtgcctg cccgacatat ttcagcgaat tctttttggc
      781 aaaaagaatc ataaatgcgc ctataaatat agctatcgga gctatcccgg taagattcag
      841 cgaaacaaga acgcttgtgg ttgtggtacc gatatttgca cccataataa cgcctatcgc
      901 ctgcgttaaa ttcataagac ccgtattaac aaagcccaca accataaccg tggttgccga
      961 ggagctttgt ataagcgttg ttatgagaaa gccgacaagt accgcaagaa agcggttttt
     1021 tgtcattttt tcaagtaaat gcttaagacg cgacccggct gcaagctcaa gtccctcgcc
     1081 catcagctta ataccgtaca aaagcaagcc gagaccgcct gcaaaggtca gataattagt
     1141 aatactcaaa ttttttcccc tttaaaatac tcagttttcc gcttctctgc gtttttcgct
     1201 gagcttgaat tccttgccgt cttcacccat gtgatatatt gttttgctca cggaaatacc
     1261 gcttttaccg gttacccttg aatagttaac gccttttttg cgaagataag ttttatacca
     1321 ctcaagtctt cttaaaaagt ccttgtatct tctgttttca agatttgtcc acgcgacctc
     1381 ggatagtgcc aaaatacgcg gatagacgga aaactccaat gcacgctccg aatctaccca
     1441 ttcagtccac atttcggctt ctatgcctat tacgcgctgc ccggattttc cgaacccgga
     1501 tttaacggta ttatatttat acactttttt aagcgatata acagaatatt tgcagtcaaa
     1561 gtaaa
//
LOCUS       DBMDVO010000345         1564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38030_length_1564_cov_1.723855, whole genome shotgun sequence.
ACCESSION   DBMDVO010000345 DBMDVO010000000
VERSION     DBMDVO010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38030_length_1564_cov_1.723855"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="ACZPE2_05415"
     CDS             <1..585
                     /locus_tag="ACZPE2_05415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1319320.1"
                     /translation="DIHPDILVNNAGILPKFDRFEHYSMADIEKAMNINFYSAVYSIH
                     TLLPMILKSKTPGIINIDSSASLMSLAGTSVYSASKAALKSLSESLREELRGKCYVGI
                     VCPGFTKTDIFRSQDASDAKGQKMLNAVSTSCDRMVKMIMHDIRTRRPLGVHGFDAAF
                     MNYFGRLMPVLGGHIFSSVMKISKLPLFDPVFKD"
     gene            634..>1564
                     /locus_tag="ACZPE2_05420"
     CDS             634..>1564
                     /locus_tag="ACZPE2_05420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1319321.1"
                     /translation="MKIFMIGGTGLLGCEAATTLIKRGHQVKSVALPPLPEGAPIPKE
                     MELVFGDINKKTDEEIEQMLEGCDCFIFAAGVDERKEFPAPVMDYYYKYNIAPLERIF
                     PLCKKAGVKRAVVLGSYFSYLSKIKPDMNLEERNPYFKSRLIQEDVCKKACDDNFSVA
                     VLELPYIFGTQPGRRPVWTVLIEQISGMDKLPFTLYPKGGTAMLTCRQVGQAIAGAAT
                     KEGAKGFEAIPISMYNMKWDKFLGIVYEARGMHNRKIVGIPPFMMKLGMYGIVKDYKK
                     RGIDSGMDPLQLPYIMDYDLFITDKYTRDLGVED"
BASE COUNT          421 a          339 c          361 g          443 t
ORIGIN      
        1 gacattcatc ccgacatact tgtcaataat gcgggaattt tgccgaagtt cgacagattt
       61 gaacattatt caatggctga tatcgaaaag gctatgaata tcaatttcta ttcggcggtt
      121 tattcgatac atacgcttct tccgatgatt ctcaagtcaa agactcccgg aatcatcaac
      181 atcgatagct cggcttctct tatgtctctt gcaggtactt cggtatattc cgcaagtaag
      241 gctgctctta agtctctttc agaatcactc agagaagagc ttcgcggcaa atgctatgtc
      301 ggcatagttt gccccggctt tacaaagacc gatatattca gaagtcagga tgcttccgac
      361 gcaaagggcc agaagatgct caatgctgtc agcacctctt gcgacagaat ggtaaagatg
      421 ataatgcatg acatcagaac aagacgtccg ctcggcgttc atggctttga cgctgcattt
      481 atgaattatt tcggcagact tatgccggta ctcggcggcc atattttcag cagcgttatg
      541 aagatttcta agcttccgct ctttgacccc gtattcaagg actgatttag tttttaattt
      601 agcaaaaata tatagttaga caggagaatt gctatgaaaa tctttatgat cggcggtaca
      661 ggccttctcg gctgtgaagc tgccacgact ctcattaaga gaggtcacca ggtaaagagc
      721 gttgctcttc cgcctcttcc ggagggagct cccattccga aggaaatgga actcgttttc
      781 ggcgatatca acaagaagac agatgaggaa attgagcaga tgctcgaagg ctgtgattgc
      841 tttatcttcg cagcaggtgt tgacgagaga aaagaattcc cggcgcccgt aatggattat
      901 tactacaaat acaacattgc tccgcttgag agaatcttcc cgctctgcaa aaaagcaggc
      961 gttaagagag ctgttgttct tggttcttac ttctcatatc tttctaaaat caagcctgat
     1021 atgaatcttg aagagagaaa cccctacttc aaatctcgtc ttattcagga agatgtttgt
     1081 aaaaaggctt gcgatgacaa cttcagcgta gcagttcttg agcttcctta catattcggt
     1141 actcagccgg gcagaagacc tgtttggact gttcttattg agcagatttc cggtatggac
     1201 aagcttcctt tcacccttta tccgaagggc ggcacagcaa tgcttacttg ccgtcaggtt
     1261 ggtcaggcta ttgccggtgc agcaaccaaa gagggtgcca aaggctttga ggctattccg
     1321 atcagtatgt acaatatgaa gtgggataaa ttcctcggta ttgtatatga ggctcgcgga
     1381 atgcataaca gaaagattgt cggcatacct ccgtttatga tgaagctcgg tatgtacggt
     1441 attgttaaag attataagaa gagaggcatt gattccggta tggatccgct tcagcttccc
     1501 tacattatgg actatgatct cttcataacg gataagtaca cccgtgacct cggcgttgag
     1561 gatg
//
LOCUS       DBMDVO010000346         1558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38257_length_1558_cov_1.181881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000346 DBMDVO010000000
VERSION     DBMDVO010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38257_length_1558_cov_1.181881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /locus_tag="ACZPE2_05425"
     CDS             <1..>1558
                     /locus_tag="ACZPE2_05425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1319322.1"
                     /translation="TLTQQLIKNLTGENGKTVNRKFYEILSALNLEKNASKQTILEAY
                     MNTVYMSHGCYGVQTASEKYFGKNVQDLNLAECASLAAITQSPSKYDPLIHPEENKKR
                     QKVCLDNMLKQGKITEDQYNEAINYTMVFTNSEGYVPSENLTSAQQQTTTTIQSYYVD
                     YVIQKVISDLMDKYGYTKPQASTLIYSGGLRIYSAVDMNVQNILENVYVNRISFPSYN
                     KNVPDAQSAMTIMDYSGRVLGMIGGAGEKTENRGLNRAANSYRQPGSSIKPLSVYTPA
                     IEEKYAYWSTRVKNYGIPHYYSDGGVGPVNYGNDPGSPDSYVNVQKAICKSYNTVPAQ
                     LLKKMGYELSFKYANGKFRLDHLYDVDKNASSLAVGGTSKGVSTLQMAAAYATFGNGG
                     KYYDPYCYYKVTNSSGTMVYLQHDETDGDQIMSQDTADIMNELLQTVVTDTAGEATAR
                     NYGLNNMKLFAKTGTTTEDKDRWFCGGSPYYVAAVWYGCDTPKQLSNYVSGNPAGKIF
                     DEVLNRVHKGL"
BASE COUNT          479 a          335 c          350 g          394 t
ORIGIN      
        1 acccttactc aacagcttat taaaaatctt acgggagaaa acggcaaaac cgtaaatcgt
       61 aaattttatg aaatactgtc tgcgctgaac cttgagaaaa atgcctctaa gcagacaata
      121 cttgaggcgt atatgaatac ggtctatatg agccacggct gttatggcgt acagaccgcc
      181 tccgaaaagt atttcggaaa aaatgtacag gaccttaatc ttgcggagtg tgcgtctttg
      241 gcggcaataa ctcaatctcc gtcaaaatat gatccgctta ttcatcccga agaaaacaaa
      301 aaacgtcaaa aggtctgtct tgacaatatg cttaagcagg gcaaaataac agaagaccag
      361 tataatgaag caataaatta cacaatggtg ttcacgaaca gtgagggcta tgtgccgagc
      421 gaaaacctta caagcgctca gcagcagacc actaccacca ttcagagcta ctatgtcgat
      481 tacgttattc aaaaagttat ctcagacctt atggataaat acggctatac aaagccgcag
      541 gcgtctacgc tcatttattc gggcggactt cgcatctact ccgctgtgga tatgaatgtt
      601 cagaatattc tcgaaaacgt atatgtaaac agaatatcat tcccgtccta taataaaaat
      661 gttcccgatg ctcagtcggc tatgacaatt atggattaca gcggcagagt gcttggtatg
      721 ataggcggag ctggagaaaa gaccgaaaac cgcggactta accgtgcggc aaattcgtat
      781 aggcagcccg gttcatcaat aaagcctctc tctgtttaca caccggcaat agaggaaaaa
      841 tatgcttatt ggagcacaag agttaagaac tatggtattc ctcactatta cagcgacggc
      901 ggagtgggtc ccgtcaacta cggtaatgat cccggttctc ccgattcgta tgtaaacgta
      961 caaaaagcta tttgtaagtc ttacaatacc gtaccggcgc agcttcttaa aaagatgggt
     1021 tatgaattga gctttaagta tgccaacggc aaattcagac tcgatcatct ctatgatgtt
     1081 gataagaacg cctcgtcgct tgccgtaggc ggtacaagta agggagtatc aactcttcag
     1141 atggcagccg catatgccac cttcggaaac ggcggtaaat attacgaccc gtactgttac
     1201 tataaggtta caaattcaag cggtactatg gtataccttc aacatgacga gaccgatggc
     1261 gatcagatta tgtctcagga tactgcggat attatgaatg agcttctgca aaccgttgtt
     1321 accgatacgg caggtgaagc taccgcgcgc aattacggcc ttaacaatat gaaactgttt
     1381 gcgaaaaccg gtaccacaac cgaggataag gatcgttggt tctgcggagg ttcaccgtat
     1441 tatgtagctg cagtttggta tggctgcgat actcctaagc agctttcgaa ttatgtttcg
     1501 ggcaatccgg cgggtaaaat atttgatgag gttttgaaca gagttcataa aggactcg
//
LOCUS       DBMDVO010000347         1555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38351_length_1555_cov_1.119670, whole genome shotgun sequence.
ACCESSION   DBMDVO010000347 DBMDVO010000000
VERSION     DBMDVO010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38351_length_1555_cov_1.119670"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(184..1050)
                     /locus_tag="ACZPE2_05430"
     CDS             complement(184..1050)
                     /locus_tag="ACZPE2_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR1319323.1"
                     /translation="MTIALDVIKDTLIDNLKTLPFLFLAFLLLEALEHHAGERINNAL
                     RSAGKAGPLFGSLLGCIPQCGFSVFAANLYSGGVITLGTLLAVFLATSDEALIILISE
                     PSRGKDILLLLLFKIIIGITAGYIVDLFVKKHPEKEKHDIGDICKGEHCHCDEHKGFF
                     VPALTHTLKVFGFLLVFTLALNFAIELLGADRLSEILLGNTFFQPLIAALIGLIPNCA
                     ASVLLTEMYLGGALSFASVIAGLCSGAGIGLMVLFRTNKKPRENLKILVLLYGISAFS
                     GVIIEIFTKLMA"
     gene            complement(1047..1466)
                     /locus_tag="ACZPE2_05435"
     CDS             complement(1047..1466)
                     /locus_tag="ACZPE2_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1319324.1"
                     /translation="MSYNTKQSKMVFDILLENKDRHLTADEIFEKLRKSGESVGKTTI
                     YRHLEKLCASGEVRKFTGGDGDSACYQYAGKNAECREHYHLKCTECGKLIHAECKFLS
                     ELSAHIYNEHGFKVDGSRTVLYGVCGDCLKKERQLHR"
BASE COUNT          404 a          378 c          375 g          398 t
ORIGIN      
        1 cacggacccc aacgcgtcaa tgaaggagcg catcattaaa acgagcgaga aaaaaggttt
       61 atatgcggca atggacattg ctgaaatttg gctgcaaaga gcactcgcac acgaataaga
      121 aaaagcgccg taccgagaaa tcggtacagc gctttatttt ttttctttta aggttctgct
      181 cgcttaagcc atgagctttg tgaatatttc gattataacg cccgagaacg ctgagattcc
      241 gtagagcagt acgagaattt tcagattttc acggggtttt ttatttgttc tgaacagcac
      301 cataagacct atgcctgctc cggagcaaag ccccgctatt accgaggcaa agctcaaggc
      361 tccgccgaga tacatttccg ttaaaagcac ggaggcggcg cagttcggaa taagccctat
      421 aagcgcggct ataagcggct ggaagaaggt gttgccgaga agtatttcgg aaagtctgtc
      481 ggcgcccaaa agctctatgg cgaaattcag cgcaagcgtg aaaacaagca aaaagccgaa
      541 aaccttcagc gtatgtgtga gagccggcac aaaaaatccc ttgtgctcgt cacagtggca
      601 gtgctctccc ttacatatgt cgccgatgtc gtgcttttcc ttttcggggt gcttttttac
      661 aaacaggtca acaatgtaac ctgcagtaat gccgatgatt attttaaaca gcagaagaag
      721 caatatgtcc ttgcctcgtg acggctcgga aatgagtatt ataagcgcct cgtccgatgt
      781 cgcaagaaat accgcaagaa gcgtgccgag cgttataaca ccgcccgaat ataaatttgc
      841 ggcgaaaacc gaaaagccgc actgcggaat acagccgaga agcgagccga aaagcggccc
      901 tgccttgccc gcagagcgaa gagcgttgtt aatcctttca ccggcgtggt gctcaagagc
      961 ctccaaaagt aaaaatgcga gaaacaaaaa cggaagcgtc ttgaggttgt caataagagt
     1021 gtccttgatt acgtctaaag ctattgtcat ctatgaagct gcctttcctt tttcagacag
     1081 tcgccgcaca ctccgtataa aaccgtgcgc gaaccgtcaa ccttaaagcc gtgctcgttg
     1141 taaatgtggg cggaaagctc gctcaaaaat ttacattcgg cgtgtatgag cttgccgcat
     1201 tcggtgcatt tgagatggta gtgctcgcgg cattcggcgt ttttgccggc gtattgatag
     1261 cacgcgctgt cgccgtcgcc gcccgtgaat tttcgtacct cgcccgaggc gcacagcttt
     1321 tcaagatgtc ggtaaatcgt tgttttaccg acactctccc cgctttttct gagcttctcg
     1381 aaaatctcgt ccgccgtcag atggcggtcc ttgttttcga gcagtatgtc aaataccatt
     1441 ttgctctgct ttgtgttgta gctcaaattt tacgcctccg aacaatcagt ctttcacgtt
     1501 gtatctttta cgttgtatct tacacgttat gttttgcacg ttttgccgag caaag
//
LOCUS       DBMDVO010000348         1554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38385_length_1554_cov_1.247075, whole genome shotgun sequence.
ACCESSION   DBMDVO010000348 DBMDVO010000000
VERSION     DBMDVO010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38385_length_1554_cov_1.247075"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="ACZPE2_05440"
     CDS             complement(<1..454)
                     /locus_tag="ACZPE2_05440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1319325.1"
                     /translation="MNKNKLNRLDEMMQSQVDSGMIPGGHLRIIKDGERVYDKCFGMA
                     DVARNLKVSDNTIYRLYSMTKPVTAVATMILYDRGLIDLSDNVSWFLEGFKDQKVETA
                     DGIVPANREVKIIDLLTMTAGLMYPDRNVNPSGDKMADLFDKFYERAFS"
     gene            complement(571..1275)
                     /locus_tag="ACZPE2_05445"
     CDS             complement(571..1275)
                     /locus_tag="ACZPE2_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319326.1"
                     /translation="MKSTYIKLIAVSLILSCTAVLTACSSSSTSSDASTGNVGDTTPV
                     SESSATSSEAVNSESKTDSSSEDTKSSDENKSGENSTESTVTYEYVKDGKFSLANGNL
                     SFKMPKDWKLASASIAYQFASTDEYNENKFNLVVSKTNSPIEDVTSEQMTATYKATME
                     DFKLVDFQHTEIAGKKAIYMQLTGMVSQVTKQTTITQYMIQSGEDAYCFSFTQSTEDE
                     TFPDLITGVIDSLEIK"
BASE COUNT          398 a          315 c          342 g          499 t
ORIGIN      
        1 cggaaaatgc tctttcgtag aatttatcaa ataaatctgc cattttatcg ccggagggat
       61 ttacgtttct atcagggtac ataagtcctg cggtcattgt aagcaggtcg atgattttga
      121 cttcacggtt tgccggaaca atgccgtctg cggtttcgac cttctgatcc ttaaagccct
      181 caagaaacca gcttacgttg tcggaaaggt caataagccc tctgtcatag agtatcattg
      241 tcgcaactgc ggtaacgggc tttgtcatac tgtaaagacg gtatatcgta ttgtctgata
      301 ccttaagatt acgggcaaca tcagccattc cgaagcattt atcgtatact cgctctccgt
      361 ccttgattat tcttaaatgt ccgccgggaa tcattccgct gtctacctgc gactgcatca
      421 tttcatcaag gcggtttaat ttattcttgt tcatagtaac tcctgtttgc cttacggctg
      481 ttattttcag atttattgta gcatatccga tgttgaaatg caacggtaaa aaggaaaaga
      541 aacagctttt acagctgttt ctcttttgat ttacttaatt tcaagcgaat ctataacgcc
      601 tgttataagg tcggggaaag tttcatcttc agttgactgt gtgaatgaga aacaataagc
      661 atcttcgccc gactggatca tatactgtgt tatggtggtc tgctttgtta cctgtgacac
      721 catgcctgtc agctgcatat agattgcctt tttgcctgct atttcggtgt gctggaaatc
      781 aaccagcttg aagtcctcca tagtagcctt atatgtggcg gtcatctgct ctgaggtcac
      841 atcttcgata gggctgttgg tcttgctgac tacaagattg aatttattct cgttgtattc
      901 gtccgttgac gcaaactgat atgctataga ggcacttgcg agcttccagt ccttaggcat
      961 tttgaatgac aggttgccgt ttgcaaggga gaatttgccg tcctttacat attcgtatgt
     1021 gacggtcgat tcggtgctgt tttcgccgct tttgttctcg tcactgctct tggtatcctc
     1081 tgaactgctg tcggttttgc tttcgctgtt gacagcttcc gatgatgtgg cggaggattc
     1141 cgaaacgggt gttgtgtcac caacattacc agttgatgca tcagatgagg tcgaggacga
     1201 actgcaggcg gtgagcaccg ctgtgcagga gagtatcaga gatacggcaa tgagttttat
     1261 ataagttgat ttcatactga aattcctttc gataatcaaa tagtcagccg ataggcttta
     1321 taaatgatat tttagcacat aaggcgtaaa aagtcaattt ctaaccttta taaaaggcaa
     1381 ttgtgaaaga aatttcattt atttttcgta ttgttccaaa acccaagcaa gtttcataga
     1441 atataacaaa aacatattgc ggaggttaag ctatggaaaa atgtacattc agcacggtta
     1501 cgcagaaata tcttgacagc tatgcaggca tacttgatac tatgacggga acga
//
LOCUS       DBMDVO010000349         1553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38411_length_1553_cov_1.668044, whole genome shotgun sequence.
ACCESSION   DBMDVO010000349 DBMDVO010000000
VERSION     DBMDVO010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38411_length_1553_cov_1.668044"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(43..>1553)
                     /locus_tag="ACZPE2_05450"
     CDS             complement(43..>1553)
                     /locus_tag="ACZPE2_05450"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1319327.1"
                     /translation="DVPSIETPGVSVLDEYYWLNKHDPNYSLCRATVRCGEDAHTDKK
                     FELDKESAMALSQLFLTQEKDLEDKKISDILPDSFWSTNFWLYWQTMFAFQRWSSALE
                     MKRYLCRYVHHIDGLPDFSALRFTKYNQYDSMILPLVKYLEAHNVRIEYGMDVKNVII
                     ETVGDKKIAKQIVYVKDGKEQTIDLVEDDLVFITNGCCTDTSCYGDQTHAPDLSGVKN
                     GAGESWDMWKNIAKQAAHGEFGNPDAFCSDVEATNWMSATVETSNEEVIRHIMKICKR
                     DPREGKVTTGGIVTVKDSTENWYLSWTINRQPQFKSQDKNAVLIWLYSLSTNKEGNFV
                     KKAMRDCTGEEVCREWLYHIGVPTDEIEDLAKNACNTTTCFMPYINAFFQPRKNEDRP
                     KVVPDGAVNFAFIGQFAETPRDTIFTTEYSMRTGMESVYTLLNVDRGVPEVWGSKYDV
                     RELLRACYYAIDKKPITDIKLSFKEKELLKIVMKKVKGTDIELLLKESGLIK"
BASE COUNT          358 a          375 c          359 g          461 t
ORIGIN      
        1 ggactgtaac ttttgttaca gtccctttta aattacgggg ccttacttta tgagtccgct
       61 ctctttaaga agcagttcaa tatcggtgcc cttaaccttt ttcataacta ttttaagaag
      121 ttctttttct ttgaaggaaa gcttaatatc ggttatgggc tttttatcta tcgcatagta
      181 gcaggcgcgc agaagctctc tcacgtcgta tttactgccc caaacctcgg gaacgcccct
      241 gtcaacatta agaagcgtgt agacggattc catacctgta cgcattgagt attccgtagt
      301 gaatatggta tcgcgcggag tctctgcgaa ctgaccgata aaagcgaaat tcacggcacc
      361 gtccggcacg accttcggtc tgtcctcgtt cttcctcggc tggaagaatg cgttgatgta
      421 aggcataaag cacgttgttg tgttgcaggc attctttgcc aaatcttcta tctcgtctgt
      481 gggaactccg atatgataga gccactcgcg gcagacctct tcgccggtgc agtcacgcat
      541 tgccttttta acaaagttgc cctctttgtt tgtgctcagc gaatagagcc aaatgagtac
      601 ggcattttta tcctgcgatt taaactgcgg ctgacggtta atggtccaag agagatacca
      661 gttttccgtg ctgtccttta cggtgactat gccgcctgtc gtaaccttac cttcgcgggg
      721 gtcacgcttg cagattttca taatatgacg gataacttct tcgtttgacg tctctaccgt
      781 ggcgctcatc cagtttgtcg cttcaacatc gctgcagaaa gcatcgggat tgccgaattc
      841 gccgtgtgcc gcctgttttg ctatattctt ccacatatcc cacgactcgc ctgcgccgtt
      901 tttaacgccc gacaggtcgg gagcgtgagt ctggtcgcca tagcaggatg tgtcggtgca
      961 gcatccgttt gtaataaaca caaggtcatc ctcaacaagg tctatggtct gctccttgcc
     1021 gtccttaaca taaactatct gctttgcgat tttcttgtcg ccgacggttt cgattattac
     1081 atttttaaca tccataccgt attcgattct gacattgtgt gcctcaagat attttacgag
     1141 cggcaaaatc atactatcgt actgattata ttttgtaaaa cgaagtgcac tgaaatcggg
     1201 aagtccgtca atatgatgaa cgtagcggca aagataacgc ttcatctcaa gtgcgcttga
     1261 ccaacgctga aaagcgaaca tagtctgcca gtagagccag aagtttgtgc tccagaacga
     1321 gtcgggaagt atatcggaaa tctttttgtc ctcaaggtct ttttcctgtg tgaggaaaag
     1381 ctgtgaaaga gccattgccg actccttgtc aagctcaaat tttttgtcgg tgtgtgcgtc
     1441 ctcgccgcat cttaccgtgg cacggcagag cgaatagttg gggtcgtgct tgttaagcca
     1501 atagtattcg tcaagcaccg atacaccggg agtttctatt gacggaacgt cgc
//
LOCUS       DBMDVO010000350         1551 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38496_length_1551_cov_0.980000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000350 DBMDVO010000000
VERSION     DBMDVO010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38496_length_1551_cov_0.980000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(649..>1551)
                     /locus_tag="ACZPE2_05455"
     CDS             complement(649..>1551)
                     /locus_tag="ACZPE2_05455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1319328.1"
                     /translation="NKKFSYRAPSDHFGDSFLLKVKVHPEDNDRYHKDLEKLSKGEEF
                     EGNEYRWKNIYGDYIWILLRTATIRDRDGNIAKIVGVIVDIDRAKKSEKLLTERASYD
                     SLTGLYNRESIERTIDNEIELINVRKSEVAILFIDVDDFKIYNDKYSHATGDQVLKFV
                     ANTINFVIKGFGTAGRYGGDEFVACVRNIETNEPTRVARDILSGLKEGFTSDNGDKLS
                     VNASIGISIIKDSSMPVDEIIGMADDAMYKIKKNGKSNFGILNKEMVERPVPAKVEEE
                     DVIGTVEETTAQANGAQSDAPVHD"
BASE COUNT          394 a          344 c          301 g          512 t
ORIGIN      
        1 aagtgtaaat ttcaagaaat ctttcattat gcttattgtt tttttgagcc agcttattat
       61 taagctaaca aagataaata attgagttgt atttatccaa taagtcgcaa gattttttgt
      121 tttatcacag tggaacgata gattgccttt ggaaataaac gaatcgtacg gatgtcatga
      181 gagggatttt ttttatcttt gtcgctgtca ttatgcgaca ttaatttaac attatattta
      241 ctatgaaaag aagaatccct cttatggctg ctgctttggc aggtttattt ttctattctt
      301 cctgttctcc gacagaagca acataggtca gatactctgc cgcacttgaa cgaatgttta
      361 tttcattttt cttttttgcc aacgcaacca cctccgatta tttagattgt gccaccggtg
      421 gtgacacttt tagtactgaa tcacgagtaa aaaccggaaa cttatatccc taaaatataa
      481 aatccgttat atgttatagg ttcttccatc catgcaacca gttcttcgta atcccttatt
      541 ttttcagaaa ttacacgttc cataatgcct tctcgccgat acagcaagga gtgctgtcag
      601 aaacagcact ccttttgaat ttataaccga tttatttcgt gttatggatt aatcgtgtac
      661 aggtgcatcc gactgagcac catttgcctg agcagtcgtt tcttcaactg tgccgataac
      721 atcttcctcc tcaaccttag caggaacagg tctttcaacc atttccttat tgagaatacc
      781 gaagttcgac ttaccgttct tcttgatctt gtacattgcg tcatcagcca taccgattat
      841 ctcgtcaacg ggcatcgaag aatccttgat gattgaaata ccgatacttg cattgacgct
      901 gagcttgtcg ccgttatccg atgtaaagcc ttccttaagt cccgacagaa tatcacgggc
      961 tactcttgta ggctcgtttg tttcaatgtt tcttacgcag gctacaaatt cgtcgccgcc
     1021 gtaacgaccc gcagtaccga atcccttgat tacaaaattg atcgtatttg caacaaattt
     1081 cagtacctgg tcgcctgtag cgtggctgta tttgtcgttg taaatcttga agtcgtcaac
     1141 gtcaatgaac aggattgcta cctcgctctt gcggacattg ataagctcaa tttcgttatc
     1201 aatggtgcgc tcgatggatt cacggttata aagacctgta aggctgtcgt agcttgctct
     1261 ttccgtaagg agcttttcac tcttctttgc acggtcaata tcaacgataa cgccgacaat
     1321 cttggcaatg tttccatctc tgtcacggat agtcgcagta cggagcagta tccagatata
     1381 gtcgccgtag atgttcttcc agcgatattc gttgccttcg aattcctcgc cctttgacag
     1441 cttttcgaga tccttgtgat aacggtcgtt gtcctcggga tgaaccttaa ccttaagaag
     1501 gaagctgtca ccgaagtggt cggagggtgc acggtagctg aacttcttgt t
//
LOCUS       DBMDVO010000351         1548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38589_length_1548_cov_1.414651, whole genome shotgun sequence.
ACCESSION   DBMDVO010000351 DBMDVO010000000
VERSION     DBMDVO010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38589_length_1548_cov_1.414651"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="ACZPE2_05460"
     CDS             <1..758
                     /locus_tag="ACZPE2_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319329.1"
                     /translation="EFKNVSFTYPSAEKSTVCNMSFKVNRGENIAIVGENGAGKTTAV
                     KLLCGLYYPTSGEILINGKSSRDFSSDSYFNLFSAVFQDYYFMPMTIAENICTASDYD
                     RKKLFAAFDKAGISEKINSLPLKEKTYMIKDVYKDAADFSGGEKQKLLLAKAIYKNAP
                     VLILDEPTAALDPIAENELYLKYNEMTSGKISFFISHRLSSTRFCDRILLVKDGKIAE
                     SGTHEELMALKGLYYRMYQVQSYYYKENGGETV"
     gene            755..>1548
                     /locus_tag="ACZPE2_05465"
     CDS             755..>1548
                     /locus_tag="ACZPE2_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319330.1"
                     /translation="MNRVKTVFAAFREMNRLEKRLIPTAVTVAFVTAVMPFINIWFTS
                     KIIDLLDGGAGMKSLTLYIGSAVGINAVLFFVNCFLGEMQFVYRSLMYNKELREISSK
                     LFRIEYQRLENSEFKELVHKHSEAQDRVFSSFVQFTWMMRDFISGALTLVISVVIIIP
                     LLKIGFEKTGTSFFERPAFLLTIFGAIAVMAVIILVVAVRMNKAWFKASDEYSRLDRI
                     FYYFLNMFSDYNTGKEIRVYNEQTLIKHTATDKLLTDGERVLKKAS"
BASE COUNT          432 a          317 c          358 g          441 t
ORIGIN      
        1 ttgaatttaa gaacgtttca tttacatatc cgtccgccga aaaaagcacg gtttgtaata
       61 tgtcatttaa ggtgaacagg ggtgaaaaca tagccatagt cggcgaaaac ggagcaggca
      121 aaacgaccgc cgttaagctg ctttgcggct tatattatcc cacttccggt gagattttga
      181 ttaacggaaa aagcagtcgt gatttttcaa gcgacagcta ttttaacctc ttttccgcag
      241 tttttcagga ctattatttt atgccgatga caattgcgga aaatatttgt actgcctcgg
      301 actatgacag aaaaaagctt tttgccgcat ttgataaagc gggtatttcc gaaaaaatca
      361 acagtctgcc gctaaaggaa aaaacctata tgataaagga cgtgtataag gacgcggctg
      421 atttctccgg cggtgaaaag caaaagctgc tcctcgcaaa agcaatatat aaaaatgcac
      481 ctgttttgat tttggacgag cccacggcgg cactcgaccc cattgcggaa aacgagctgt
      541 acttgaaata caacgagatg acaagcggca aaatatcgtt ttttatttcg caccgcctct
      601 cgtcgacgcg cttttgcgac aggattttgc ttgttaagga cgggaaaatt gccgagagcg
      661 gtacgcatga ggagctgatg gctctcaagg gcttatatta cagaatgtac caagtccaaa
      721 gctactatta taaggagaac gggggagaga ccgtatgaac agagtaaaaa ccgtttttgc
      781 ggcgttccgt gaaatgaacc gtctcgaaaa acgccttatt cccaccgccg ttactgttgc
      841 gtttgttacg gctgttatgc cgtttatcaa catttggttc acatcaaaaa tcatagattt
      901 gctcgacggc ggagcaggta tgaaaagtct cacactctat atcggctcgg ctgtcggtat
      961 taacgccgta ttgtttttcg tcaactgctt tctcggcgaa atgcagtttg tttatcgctc
     1021 gctgatgtac aacaaggagc ttcgcgaaat ttcgtcaaag ctgtttcgca ttgagtatca
     1081 acgtcttgaa aacagtgaat ttaaggagct tgttcacaag cattcggagg ctcaggacag
     1141 agtcttttcc tcatttgtac agtttacatg gatgatgcgt gattttatat cgggtgcgct
     1201 tacgctggtt atatccgttg ttataataat tccgctgctc aaaataggct ttgagaaaac
     1261 cggcacctcg ttttttgaac gtcccgcatt tttgcttacg attttcggcg caatagccgt
     1321 tatggcggtt attattcttg tggtagcggt cagaatgaac aaggcttggt ttaaggcgag
     1381 cgacgagtat tcgcgccttg acagaatttt ttattatttt ctcaacatgt tttcggatta
     1441 caatacgggc aaggaaatcc gcgtttataa tgagcagact ctaataaagc acaccgcaac
     1501 ggacaagctt ttgacagacg gcgaaagggt tttgaaaaaa gcttcaat
//
LOCUS       DBMDVO010000352         1546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38681_length_1546_cov_0.845675, whole genome shotgun sequence.
ACCESSION   DBMDVO010000352 DBMDVO010000000
VERSION     DBMDVO010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38681_length_1546_cov_0.845675"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="ruvB"
                     /locus_tag="ACZPE2_05470"
     CDS             complement(<1..823)
                     /gene="ruvB"
                     /locus_tag="ACZPE2_05470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1319331.1"
                     /translation="MEKRIITTAVTENDGDMEINLRPNTLESYIGQDKVKNNLSIFIE
                     AAKRRKEPLDHVLLYGPPGLGKTTLSNIIANEMGVNIKTTSGPAIERPGDMAAILNGL
                     NEGDILFVDEIHRLNRMIEEILYPAMEDFVIDIVIGKGAGARSVRLELPKFTLIGATT
                     RIGLLTAPLRDRFGVVNHLEPYTVTDLSKIVIRSATVLNTEIDAGGAAEIARRSRGTP
                     RIANRLLKRVRDFAEVRYDGLITCDIASEALDMLDVDKVGLDVIDRKILLTIIDKF"
     gene            complement(836..1447)
                     /gene="ruvA"
                     /locus_tag="ACZPE2_05475"
     CDS             complement(836..1447)
                     /gene="ruvA"
                     /locus_tag="ACZPE2_05475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1319332.1"
                     /translation="MISYIKGEITFNEGDTLVVENNGIGYEITVPTFSAQKLLSKGGE
                     VTVYTYMSVREDGISLFGFESREEKAFYQKLISVSGIGPKAAISILSSITPSELVTAV
                     LSSDAAAIAKAPGVGKKTAQRVILELKDKIGSGDIAEMFDMGSDISSASAATGDDRAE
                     AAEALVALGYSRSEAVKAVSKVYVPDMDVQKILSAALRELSRI"
BASE COUNT          409 a          393 c          271 g          473 t
ORIGIN      
        1 cgaatttatc tattattgta agaagtatct ttctgtcgat aacatcaagt cctaccttat
       61 ccacatcaag catatcaagt gcctccgacg ctatatcaca ggtgataagt ccatcatagc
      121 gcacctctgc aaaatctctt actcttttta aaagcctgtt tgcaatcctc ggtgttcctc
      181 ttgaacgtct tgctatttct gccgcacctc cggcatctat ctccgtattg agcacagttg
      241 cggaacgtat aacaattttg gacaagtccg taaccgtata cggctcaaga tgattaacga
      301 caccgaaacg atccctgagc ggtgcagtca aaagaccaat tcgtgttgtg gcaccgataa
      361 gcgtgaactt aggcagttca agacgcacac ttctcgctcc tgcacctttt ccgataacaa
      421 tatctataac aaaatcctcc attgccggat aaagtatttc ctcaatcatt cggttaaggc
      481 ggtgtatttc gtccacaaaa agaatatcac cttcattaag gccgttcagt attgccgcca
      541 tatcgccggg acgttctatt gccgggcccg atgtggtttt tatattaacg cccatttcat
      601 tggcaataat atttgaaaga gttgtttttc ccagacccgg aggtccatag agcaaaacat
      661 ggtcaagcgg ctcttttctt ctttttgccg cctcaataaa tatacttaaa ttatttttga
      721 ccttatcctg tcctatatag ctttccaacg tattcggacg aagatttatt tccatatcgc
      781 cgtcattttc ggtaacggca gttgttataa ttcgtttttc catagccatt ctccgttaaa
      841 tcctgctaag ctcacgaagt gccgcagaca atattttctg tacgtccata tcgggtacat
      901 ataccttgct tacagccttt acagcctcgc ttctcgaata tccgagtgca acaagagcct
      961 ctgccgcttc tgccctgtca tcaccggttg ccgcagatgc agacgaaata tccgagccca
     1021 tgtcaaacat ttccgcaata tcgccgcttc cgattttgtc cttaagctca agaataactc
     1081 tctgtgcggt tttctttcct actcccggtg ctttcgcaat agccgccgca tcggaggaaa
     1141 gtacagccgt aaccagctcc gacggcgtta tagagcttaa tatgcttatt gccgccttag
     1201 ggcctattcc ggaaacggaa ataagcttct gataaaaagc cttttcttca cggctttcaa
     1261 agccgaagag cgaaattccg tcctctctca cactcatgta tgtataaacc gtaacctctc
     1321 cgcctttgga caaaagcttt tgtgccgaaa aggtcggtac ggttatttca tatccgattc
     1381 cgttgttttc aacaacgagt gtatcacctt cattgaaggt aatttctcct ttaatataag
     1441 aaatcatttc catgctccta atttaatatt ctatcattat tataacgaat ataaggcata
     1501 aatacaacga aaaccagttc gatttttcaa tataaaaatt ccttcc
//
LOCUS       DBMDVO010000353         1544 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38740_length_1544_cov_1.262647, whole genome shotgun sequence.
ACCESSION   DBMDVO010000353 DBMDVO010000000
VERSION     DBMDVO010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38740_length_1544_cov_1.262647"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..621)
                     /locus_tag="ACZPE2_05480"
     CDS             complement(61..621)
                     /locus_tag="ACZPE2_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR1319333.1"
                     /translation="MSEETVINCCSPCLAGLKTGNLFSCKVDDKSDLLRSVRELNRRL
                     VPRGLRIMPVKITKTRALIYLYRPDRLEKDLADKTAAELLSERAYPVGCTERCVAELI
                     RRVRHEEDFPHEIGLFLGYPSEAVSGFITHGAKNSKCVGTWKVYGDEAEAQKKFKLYK
                     KCTRVYKDAYKKHNSFERLVVSSHNK"
     gene            complement(628..759)
                     /locus_tag="ACZPE2_05485"
     CDS             complement(628..759)
                     /locus_tag="ACZPE2_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319334.1"
                     /translation="MRSLNSLDGAAINPPHRADFKKLLRLANANHVDCVADFDIREE"
     gene            complement(905..>1544)
                     /locus_tag="ACZPE2_05490"
     CDS             complement(905..>1544)
                     /locus_tag="ACZPE2_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR1319335.1"
                     /translation="WLPDYRRYYTNICGKYGMQVQSLLKKALTLDIQMICPLHGPVWR
                     SNLVYIINKYDLWSKYEAEGNSVMIAYASIYGNTENAAYYLANELSKLGVKDIAMYDV
                     SSTDVSYLISETFRCSHIVLMSATYNLEIYPKMDDYISDMKRLNISNKTFAIVDNGTW
                     APTAGKKMRETVETLKSCKICENTTTVKSAVNNSNAAALAALAKEIAAEIQG"
BASE COUNT          392 a          375 c          317 g          460 t
ORIGIN      
        1 tttcccgttc cgctccaata tactactgct accttactca ttttatcata tcctttcaga
       61 ttatttatta tgactgctca caacaaggcg ttcaaatgaa ttgtgctttt tgtaagcgtc
      121 tttatacact cttgtgcatt ttttataaag cttgaatttt ttctgtgcct ccgcttcgtc
      181 accgtaaacc ttccaagtgc cgacgcattt tgaatttttt gcgccgtgcg ttatgaagcc
      241 gctgacagcc tcagacggat aacccagaaa gagtcctatt tcgtgcggaa aatcctcttc
      301 gtgcctgaca cgtcttatga gctccgccac acatctttcg gtacagccca cggggtaagc
      361 cctctccgac agcagctcgg cagcggtttt gtcggcaagg tctttttcga gcctgtcggg
      421 tctgtaaaga tatatcagcg cccttgtttt tgtgatttta accggcataa tgcgaagccc
      481 gcgaggcaca agccgccgat tcagctcgcg aaccgagcga agcaagtcac ttttatcgtc
      541 aaccttgcag gaaaacagat ttccggtttt aagaccggca aggcatggcg aacagcagtt
      601 aataaccgtt tcttcggaca aaatcggtca ctcctctctt atgtcaaaat ccgcaacaca
      661 gtccacgtgg ttagcgtttg ctaaccgcaa gagcttttta aaatccgctc tatgcggcgg
      721 atttattgcg gcaccgtcaa gggaatttaa gctccgcata tggcaccgat ttaataattc
      781 tacgcttcaa gtcggttagc atatgctaac cgtttggtgt aaaaacttcc tgctcgactg
      841 tccgagcagg tcgtttacat atgaattata tcagattgcc gaacggtatg tcaagtacat
      901 tttatcagcc ttgtatttct gccgcaattt ccttcgccaa ggctgcaagg gctgctgcat
      961 tgctgttgtt aaccgcagat ttcaccgtag tcgtattttc gcagatttta cagcttttaa
     1021 gcgtttctac ggtctcgcgc atttttttgc cggcggtggg cgcccatgtt ccgttatcaa
     1081 ctatcgcaaa cgtcttattt gaaatattaa ggcgtttcat atccgaaata taatcgtcca
     1141 tcttgggata aatctcaaga ttgtacgtag ccgacatcaa cactatgtga ctgcagcgga
     1201 aggtctctga aatgagataa gacacgtcgg tcgaagagac atcatacatg gcgatatcct
     1261 taacaccgag ctttgacaat tcatttgcaa gataatatgc ggcattttcg gtattgccgt
     1321 aaatcgacgc ataagctatc ataaccgaat tgccctcagc ctcatattta ctccatagat
     1381 catatttatt tatgatataa acaagatttg agcgccaaac cggtccgtgc aaaggacata
     1441 tcatctgaat atcgagcgtc aaagcttttt tgagaagcga ctgcacctgc ataccgtatt
     1501 tgccgcaaat atttgtatag tatctgcgat aatccggaag ccaa
//
LOCUS       DBMDVO010000354         1543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38782_length_1543_cov_1.129681, whole genome shotgun sequence.
ACCESSION   DBMDVO010000354 DBMDVO010000000
VERSION     DBMDVO010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38782_length_1543_cov_1.129681"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="ACZPE2_05495"
     CDS             complement(<1..659)
                     /locus_tag="ACZPE2_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019149.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1319336.1"
                     /translation="MRDGQYVSARPKYGYLKDPNDCHKLIVNPETAPVVKLIFELFNG
                     GMSINEICLQLNERQIPTPGAYGYQKGYITSRKSIGSGLWQTMTVRQILTHEIYTGKL
                     VQGRSDSLAKKQTAVDKDEWIVVPNTHEAIISQDMFDKAQERMEQLKNKHSKLTVIPY
                     TENIWKGKIFCGECGRALHRSRFSRVKTGFAYNISCLTNTRYKRDGCDNGSILETDLL
                     NV"
     gene            complement(663..1172)
                     /locus_tag="ACZPE2_05500"
     CDS             complement(663..1172)
                     /locus_tag="ACZPE2_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1319337.1"
                     /translation="MARKSRKNISVAQTKPENMPIYIKTAQYIRLSVEDSHNKGNSIE
                     NQKMILDDFISQNGNMRLAGVYIDNGATGTSFHRPEFQHMIEDIESGKIDCVIVKDLS
                     RLGRNSIDTGFYIDTYFPEKKVRFIAVNDNFDTDTPSNGDKMLLYIKNIINEAYALDI
                     GKKIKTQAR"
     gene            complement(1174..>1543)
                     /locus_tag="ACZPE2_05505"
     CDS             complement(1174..>1543)
                     /locus_tag="ACZPE2_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319338.1"
                     /translation="KQLDKTSEYKSTLYENYISGILNKEDYKTLNTSYSKGKKRLEDA
                     LKQLETELDECRNNTSKKLQWIEHFKAFENIDKIDRNIVIRLIECIHIYDKTHIQIAF
                     NFKDEYEQALAALSEYREVV"
BASE COUNT          354 a          322 c          300 g          567 t
ORIGIN      
        1 acattaagca aatctgtttc aagaatactg ccgttatcgc atccgtcacg cttatatctt
       61 gtatttgtaa gacagcttat attatatgca aaacctgttt ttactcttga aaatcttgaa
      121 cgatgtaacg ctctgccgca ctcgccgcag aaaatttttc ctttccatat attttcggtg
      181 tagggtatta cggttagctt gctgtgctta ttttttaact gctccatacg ctcctgtgct
      241 ttatcaaaca tatcctgtga aataatagcc tcgtgcgtgt tcggaacaac tatccattca
      301 tctttgtcaa cggcggtctg ctttttagcc aagctgtcgc ttctgccttg aacgagtttg
      361 cctgtatata tttcgtgtgt taagatttgc cgaacggtca tagtttgcca taatccgctg
      421 ccgatactct ttcttgaggt tatatatccc ttttgatagc cgtatgcacc cggtgttggg
      481 atttgccttt cgttaagctg caaacatatc tcgtttattg acataccgcc gttaaaaagc
      541 tcaaatatca gcttgacaac gggtgcggtt tcgggattta cgattagctt atgacagtca
      601 ttcggatctt tcagataacc gtatttcggt cttgcactca catattgtcc gtcacgcatt
      661 gcctaccgtg cctgtgtttt gattttcttt ccgatgtcca aagcgtaggc ttcgtttata
      721 atatttttga tataaaggag cattttatca ccgtttgagg gggtatccgt atcaaagtta
      781 tcgtttacgg caataaaccg caccttcttt tcgggaaagt aggtgtctat ataaaagcct
      841 gtgtcaatac tgtttctgcc gagtcttgac aagtccttta caattacaca gtctatcttg
      901 ccgctttcaa tatcctcaat catatgctga aattcggggc ggtgaaagct tgtgcctgtc
      961 gctccgttgt cgatatatac tcctgccaaa cgcatatttc cgttttgtga gataaaatca
     1021 tcgagtatca tcttttgatt ttcgatggaa ttacccttgt tgtggctgtc ctcaaccgat
     1081 aatctgatat actgtgccgt ttttatgtat ataggcatat tttccggctt tgtctgtgcg
     1141 acagagatat tttttctgct ttttcttgcc atatcaaacc acctcccgat attcgcttaa
     1201 tgcagctaat gcctgttcgt attcgtcttt gaaattaaaa gctatctgta tgtgcgtttt
     1261 atcataaata tggatgcact ctataaggcg gataacaatg ttgcggtcaa ttttatcaat
     1321 attttcaaag gctttgaagt gttctatcca ttgtagcttt ttcgaggtgt tgtttctgca
     1381 ctcgtcaagc tcggtttcaa gctgttttaa tgcgtcctcc aaccgtttct tgcctttgga
     1441 gtaggaggtg ttaagcgttt tgtaatcctc tttattcaga atgccggaaa tgtagttttc
     1501 ataaagggtt gatttgtatt cacttgtttt gtcaagctgt ttt
//
LOCUS       DBMDVO010000355         1538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38947_length_1538_cov_1.208768, whole genome shotgun sequence.
ACCESSION   DBMDVO010000355 DBMDVO010000000
VERSION     DBMDVO010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38947_length_1538_cov_1.208768"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="ACZPE2_05510"
     CDS             complement(<1..196)
                     /locus_tag="ACZPE2_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319339.1"
                     /translation="MNYSQEVEKMCCVAKGPKHGPAPIPEEGKWVKAYDIKDISGFSH
                     GVGWCAPQQGACKLSLNVKDG"
     gene            complement(303..533)
                     /locus_tag="ACZPE2_05515"
     CDS             complement(303..533)
                     /locus_tag="ACZPE2_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319340.1"
                     /translation="MTLSELSGEYLKEEEKLTRQIKSFTPEIHRLTGEDLYLARRRLM
                     CLYEMRSDVRAVARKLENYYDKGDMRPVYRKH"
     gene            complement(758..1351)
                     /gene="lexA"
                     /locus_tag="ACZPE2_05520"
     CDS             complement(758..1351)
                     /gene="lexA"
                     /locus_tag="ACZPE2_05520"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR1319341.1"
                     /translation="MKTINETQKKIYEYLMERSQCGVPPSVREIGAAVGLKSTSSVQA
                     NLDALEEAGYIERDPMLKRSIRVTGDAENVTNVPLLGTVTAGMPILAVEQIEGYLPYS
                     GRVSRDKPLFALKVRGESMIWAGILSGDIVIVEKTPVAANGEIVVAMIDDEATVKRFY
                     KENGHFRLQPENDAFKPIIVDEVIILGKVVGLMRYYD"
BASE COUNT          393 a          377 c          328 g          440 t
ORIGIN      
        1 cgccgtcctt tacattaagg ctgagcttgc acgcaccctg ctgcggagca caccagccga
       61 cgccgtgtga aaatccggaa atatctttga tgtcgtatgc ctttacccat ttgccctctt
      121 cggggatagg tgcgggaccg tgtttcggcc ctttggcaac gcaacacatt ttttcaactt
      181 cctgtgaata gtttatcatt ttgaagctcc tttcaatttg ttacggaaac attatacagt
      241 ttcgtgataa atttatcaat aggaaaagtt acaattagcc gacggttcga caaaaaatat
      301 tgtcaatgtt tgcggtaaac aggccgcata tcacccttgt cgtagtaatt ttcgagcttc
      361 cttgccacgg ctctcacgtc gctgcgcatt tcataaagac acataagccg ccgccttgcg
      421 aggtagaggt cctcgcccgt caaacggtga atttcgggag taaagctctt aatttgccgc
      481 gtaagcttct cctcctcctt taagtattcc ccggataatt cggataatgt caaatttatc
      541 gcccctttct gctgtcgaac aaatgttcgt ctgcaaatac aatattagca gaaaagggct
      601 tttcggtcaa tagaacatac caaagagcaa tgaaatgtcc tgattaacgg acttactcaa
      661 ggcttaccgt gcaaaaaaac accgactcgc ttaaaaagca aatcggtgca atgcttagca
      721 tattttgacg ggcagagggg cgtcaaaggt cattctatca gtcgtaatac ctcataagac
      781 ccacgacctt gccgagaatt atgacctcat ccactataat gggtttaaag gcgtcgtttt
      841 ccggctgaag gcggaaatga ccgttttctt tataaaacct ttttacggtt gcttcgtcat
      901 caatcatggc gacgactatt tcgccgtttg cggcaacggg agttttttcg acaattacaa
      961 tgtcgccgct gagaataccg gcccaaatca tactttcgcc gcgaaccttc agcgcaaaca
     1021 gcggcttatc gcgcgagact ctgccgctgt atggcagata gccctcaatc tgctcaacgg
     1081 cgagtatcgg cataccggcc gtgaccgttc cgagaagcgg aacattggtt acgttttccg
     1141 cgtcgccggt aacacgtatc gaccttttta acatcgggtc acgctctatg taacccgcct
     1201 cttcaagcgc gtcgaggttc gcctgcaccg aggaggtcga tttaagcccc acggcggcac
     1261 cgatttcacg gactgaaggc ggaactccgc actgcgagcg ctccataaga tattcgtata
     1321 ttttcttctg agtttcgttg atggttttca tctcactgcc cccattcgat tgcgtttttt
     1381 tcattgtaac acaaatgttc tgaaaaagca aacatttgtt tgccgaaaaa cgagaaaaaa
     1441 ataaaatttt taattttatt gtattttttt ataatttgtt cattgttaat tcacatttat
     1501 cggttatagt ttacctgtac tcgacaggaa gggagccg
//
LOCUS       DBMDVO010000356         1538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_38953_length_1538_cov_1.018789, whole genome shotgun sequence.
ACCESSION   DBMDVO010000356 DBMDVO010000000
VERSION     DBMDVO010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38953_length_1538_cov_1.018789"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="tsaA"
                     /locus_tag="ACZPE2_05525"
     CDS             complement(<1..563)
                     /gene="tsaA"
                     /locus_tag="ACZPE2_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR1319342.1"
                     /translation="MEIKPIAHIRTDFGEKFGIPRQSGLCSLLEGKIVFEPEFAKRES
                     VRGLEEFSHIWLIWQFSENPPTSSLTVRPPRLGGNKRVGVFASRSPFRPNGLGLSCVR
                     LKSIEYSSSGEPVINVFGADLCDKTPIFDIKPYVPLSDCVPDALEGYTKMTKELILSV
                     HIPEEKALLLPKNKLAALKEILQLDPRP"
     assembly_gap    951..960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <961..>1538
                     /locus_tag="ACZPE2_05530"
     CDS             <961..>1538
                     /locus_tag="ACZPE2_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319343.1"
                     /translation="YDPYITKILNRLLNESAVITSWSAKLNEAFPALASFAEANGEDV
                     GTAAGGITLVPLFIGIIMTFDNIFGVIFQPTFGKLSDNTHSRLGKRRPFVVYGAPISA
                     LLFAIIPIIAVKFNSLGFTMLFIILFVFVMSLWRAPVVALMPDLTPNELRSEGNAVIN
                     LMGGIGSALGLFAGTIVTAIYCAATGISSKSP"
BASE COUNT          418 a          357 c          325 g          428 t
ORIGIN      
        1 ggtcgcgggt ctaattgcag tatctctttt aacgccgcaa gcttattttt cggaagcagc
       61 agggctttct cctcgggaat atgaaccgat aaaataagtt cctttgtcat ttttgtatag
      121 ccctccaaag catccggcac acagtcggag agcggaacat acggcttgat gtcaaatatc
      181 ggcgttttgt cgcataaatc ggctccgaaa acatttatca caggctcacc tgaggaagaa
      241 tactcaatgc ttttgagcct tacacacgag agcccaaggc cgttcggacg aaacggggaa
      301 cgagatgcga aaacgcctac tcttttgttg ccgccgagac gcggcggacg aaccgtaagc
      361 gacgacgtcg gagggttttc ggaaaactgc catataagcc aaatatgaga gaactcttcg
      421 agtcctctta cgctctcacg ctttgcaaat tccggctcga acactatttt cccctcaaga
      481 agagagcaaa gtccgctttg acgcggtatg ccgaattttt caccgaaatc ggttcttata
      541 tgcgctatcg gttttatttc caagctcttt ttcccctttt gtaaaatgtc gtcggcacat
      601 atagttttat cataaaaacc gcctcaatac aatacttatt aacaatcaaa cgacaataaa
      661 tattaaaaaa gcgataatat cggttttgat tttacggaaa acgcttttga taaatatata
      721 taaaatttat gttatatgtg tattttctac aatcaaatat tgcaatcaca gcaataattt
      781 gttattatta cagatagtgt tataccgtat tcggaaaaac gctatcaagt ttgaaaggga
      841 gaatgaaatg gaaagtaaat atttctgtgc caaaagcaac ggcaagctga actattggca
      901 cacaatactc accggattcg gctttatggc ctcatccata gcgtgggcca nnnnnnnnnn
      961 tatgacccat acataacaaa gatacttaac aggctcctta acgaaagtgc cgttattact
     1021 tcgtggagcg caaagcttaa cgaggcgttt cccgctctcg cgtcctttgc ggaagctaac
     1081 ggcgaagacg tgggcacagc cgcaggcggt attacccttg taccgttgtt cataggtata
     1141 ataatgacct ttgacaacat cttcggagta atttttcaac cgaccttcgg caagctctcg
     1201 gacaacactc attcacgatt aggcaaacgc cgcccgtttg ttgtttacgg cgcgccgatc
     1261 tccgcattgt tgttcgcgat aattccaatt attgccgtaa agttcaattc actcggcttc
     1321 acaatgcttt tcataatttt gtttgttttt gtaatgtcgc tgtggagagc accggtggtt
     1381 gcccttatgc ctgatttgac cccgaacgaa ctgcgaagcg aaggcaacgc cgtcattaac
     1441 cttatgggcg gtatcggcag tgctctcgga ctttttgccg gaactatagt caccgcaatt
     1501 tactgcgcgg ctacgggcat ttcgtccaaa tctccgga
//
LOCUS       DBMDVO010000357         1535 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39055_length_1535_cov_1.150628, whole genome shotgun sequence.
ACCESSION   DBMDVO010000357 DBMDVO010000000
VERSION     DBMDVO010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39055_length_1535_cov_1.150628"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="ACZPE2_05535"
     CDS             <1..210
                     /locus_tag="ACZPE2_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319344.1"
                     /translation="FRKRKGVTMKKILIMVVILMGIFASVFGLAGCSEKAYGEENKVK
                     FSKFSQKERIDYVRKYLLDKERVKK"
     gene            207..920
                     /locus_tag="ACZPE2_05540"
     CDS             207..920
                     /locus_tag="ACZPE2_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319345.1"
                     /translation="MKTIIRKVGLIIMAITTTIFMGACSVLNKDTSDKVCRALNEKYG
                     ETFVVTAIGDRLNTDGAKLYVHPADNKDLLFTARINRETGVVDDNYIEEKVNYKVESI
                     VADSFDKRNISVNSRCAVVTQEPLIVENEDYTPVGFKEKYNFDHYTLYLVIRQGDFTA
                     ADLLAAFENANNSVGVELVMICYIFEPENYSACVEEMKIYAEMGATMIESHSPVSAFN
                     VIAENGVCSVTESELANII"
     gene            937..>1535
                     /locus_tag="ACZPE2_05545"
     CDS             937..>1535
                     /locus_tag="ACZPE2_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319346.1"
                     /translation="MILLAVLGLGASIVTITLLICAFAIDRDNRSFEKNKRFGKAEVT
                     AYNRVSQSDAYTLYVRIPELSYKNTYSCSHGRINVSDYPPGTIVDVMYAPKKIAGNDY
                     ADIRLIDSPPADSSGIARMLKIISIFMIIALLACIVCGCMKGSDKNKIEQYLSTKYTG
                     DFTYISDGNDVWSSKTKTYVYSDENGNSFNVKNTDGVLI"
BASE COUNT          520 a          226 c          332 g          457 t
ORIGIN      
        1 tttaggaaac gaaaaggagt aacaatgaaa aaaatattga taatggtggt gatattaatg
       61 ggaattttcg catctgtttt tggcttagca ggatgttcgg aaaaagcata cggcgaagag
      121 aataaagtta aattttcaaa attttctcag aaggaaagaa tagattatgt tcgtaaatat
      181 ctgttagata aagaaagggt gaaaaaatga aaactattat caggaaagtt gggttaatta
      241 ttatggcaat tacaactacg atttttatgg gtgcttgctc ggtactcaat aaagacacat
      301 cagacaaagt atgccgtgct ctaaatgaaa aatacggtga aaccttcgtt gttactgcaa
      361 taggcgaccg tttgaatact gacggtgcaa aattatatgt gcatccggct gataataaag
      421 acctgctttt tactgcaagg ataaaccgag aaacaggtgt ggttgatgac aattatattg
      481 aagaaaaagt caattataag gttgaaagca ttgttgccga ttcttttgat aagagaaata
      541 tatcagttaa ttcaaggtgc gcagtggtaa cgcaagaacc tcttattgtc gagaatgaag
      601 attacacgcc tgtcggattc aaggaaaagt ataatttcga tcactataca ctttatcttg
      661 ttataaggca gggcgatttt actgccgcag atttgttggc ggcttttgaa aacgcaaaca
      721 acagtgtagg cgttgaactg gtgatgattt gttatatttt tgagcctgaa aattattcag
      781 cttgtgttga agaaatgaaa atttatgcag aaatgggagc aacgatgatt gaaagccatt
      841 ctcctgtatc cgcatttaat gttatagctg aaaatggtgt atgctcagtt acagaaagcg
      901 agcttgcaaa tataatatag aaaataggag aaaaatatga ttttattagc cgtgttaggg
      961 ttaggagctt caattgttac aatcacatta ttgatatgcg catttgcaat tgatagggat
     1021 aatcgcagtt ttgaaaaaaa caaacgtttc ggcaaagccg aggtcactgc ttacaaccgt
     1081 gtaagccaat cggatgcata tactctttac gttcgtattc cggaactcag ctataaaaac
     1141 acttacagct gctctcacgg caggataaat gtaagcgatt atccaccggg aacaatagta
     1201 gacgttatgt atgctcccaa aaagattgca ggaaatgatt atgccgatat ccgtcttatt
     1261 gacagtccgc ctgcggacag ttccggtatt gcccgtatgt tgaagattat atcgattttt
     1321 atgattattg ctctattagc atgcattgtg tgtggatgta tgaaaggtag cgataaaaat
     1381 aaaatcgaac agtatctgag tacaaaatat acaggtgatt tcacatatat ttctgacggc
     1441 aatgatgtat ggtcgtctaa aacaaagact tatgtttatt cagatgaaaa cggcaacagt
     1501 tttaacgtga aaaataccga tggagttctt attga
//
LOCUS       DBMDVO010000358         1532 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39137_length_1532_cov_1.853948, whole genome shotgun sequence.
ACCESSION   DBMDVO010000358 DBMDVO010000000
VERSION     DBMDVO010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39137_length_1532_cov_1.853948"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="ACZPE2_05550"
     CDS             complement(<1..686)
                     /locus_tag="ACZPE2_05550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1319347.1"
                     /translation="MNKKSDTVNSELRDKLFIVLALTGCVSNIAGFTANAVIFGFTPQ
                     TLFCAACALVIIVSSLVGFLTGRTYVATFIILIVLGFVEFPMLYISYGASTAVYMLVA
                     EIGIITFMKPVHRVIMGAAVFVADIAAFVVRSKFPQLNMANDNMELDTLFSFIIAFLG
                     IMVAVAIVIVQFERQAKQLSEMGEELTLAVNTDPLTEIRNRRYLIKYLENQLSQDEKK
                     KHCAVLIDLD"
     gene            complement(737..>1532)
                     /locus_tag="ACZPE2_05555"
     CDS             complement(737..>1532)
                     /locus_tag="ACZPE2_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319348.1"
                     /translation="NGELIIYNRYENGGAQLAKVNSSTLKEGVMSFDVETQGGENITI
                     SQSQLVARVNYCSDFWGAVIGFAMSPFGVMAIAILPCIAIVAFEIVKFIMSKRPVPQV
                     ETVKLQEEAPVYVPPESEEHKLPKASKTEAKKAAIPSLSDNGKVPHNRIPDSSHGKTD
                     DFTERLRSKKNIAASADNIKDRADFSAGARKRMESDRAEQQKEKKAKTENIKNENALP
                     EMTEKTVAAEVTHSGSEPDISLVFRDDEDKRYDIDDILADIEKRHH"
BASE COUNT          376 a          386 c          288 g          482 t
ORIGIN      
        1 aaatcgaggt cgataagcac cgcacagtgt ttctttttct cgtcctgcga aagctgattt
       61 tcaagatatt ttataagata acggcggttt cttatctcgg tcagcggatc ggtattcaca
      121 gcaagcgtaa gctcctcgcc catttcactg agctgttttg cctgacgctc aaattgcact
      181 atcactattg cgaccgcaac cattatgcct aaaaacgcta taatgaacga aaacagcgta
      241 tcaagctcca tattgtcatt agccatattc agctgtggga atttgcttct gacaacaaat
      301 gcggctatat ctgcaacaaa caccgccgct cccattataa ctctgtgcac cggcttcata
      361 aaagtaataa ttccgatttc cgcaacaagc atatacactg ccgtgcttgc accgtatgaa
      421 atatacaaca tcgggaactc gacaaatcca agcacaatga gtattataaa cgtagcgaca
      481 taggttcttc ctgtcagaaa accgaccaat gacgatacta taattacaag tgcgcaagcc
      541 gcacagaata aggtctgcgg ggtaaatccg aatatcaccg catttgcagt aaatcccgct
      601 atattcgata cacaaccggt taatgccaat actatgaaca gcttgtcacg aagctctgaa
      661 ttcacagtat ctgatttttt attcatttca agtgttctct ctttcgttct tacttttctt
      721 tctgcttttc tgcccgtcaa tgatgccttt tttctatatc ggcaagaata tcgtctatat
      781 catatctctt gtcctcgtca tctctgaaca ccagcgatat atcgggctcg gagccgctgt
      841 gcgttacttc agccgcaacc gttttttctg tcatttcggg caaagcattt tcatttttta
      901 tattttctgt tttagctttc ttttcctttt gctgctctgc tctgtccgat tccattcttt
      961 ttcttgctcc ggcggaaaaa tccgctcggt cttttatgtt atcggcactt gccgctatat
     1021 tctttttgct ccgcagacgc tctgtgaaat catccgtttt accgtgagag ctgtccggga
     1081 tacgattatg cggcactttt ccgttatcgg ataaagacgg aatagcggcc tttttcgctt
     1141 cggtcttgct tgcttttggc agcttgtgtt cctcgctttc agggggaaca tacaccggag
     1201 cttcttcctg caatttgacc gtttccacct gcggtaccgg gcgctttgac ataatgaact
     1261 tcactatctc aaatgctact attgcgatac acggcagtat ggcgatagcc ataacgccaa
     1321 acggcgacat tgcaaatccg ataacagcgc cccagaaatc actgcaatag ttaactcttg
     1381 caacaagctg gctttgtgat attgtgatat tttctccgcc ttgagtttca acatcaaagc
     1441 tcataacgcc ttccttaagc gtggaggaat ttacctttgc aagctgtgct ccgccgtttt
     1501 cataccgatt atatattata agctctccgt tg
//
LOCUS       DBMDVO010000359         1530 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39239_length_1530_cov_1.012596, whole genome shotgun sequence.
ACCESSION   DBMDVO010000359 DBMDVO010000000
VERSION     DBMDVO010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39239_length_1530_cov_1.012596"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..367
                     /locus_tag="ACZPE2_05560"
     CDS             2..367
                     /locus_tag="ACZPE2_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR1319349.1"
                     /translation="MEELMAIRDKTRKNMTVREDTGDSKIRVVVGMATCGIAAGARPV
                     LNAFVDEIAKRDVKGVSVQQTGCIGMCQYEPIVEVTEPGKEKVTYVKMTPEKAVRVVN
                     DHIVNGNPVAEFTVGAIKD"
     gene            380..>1530
                     /locus_tag="ACZPE2_05565"
     CDS             380..>1530
                     /locus_tag="ACZPE2_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR1319350.1"
                     /translation="MYRSHVLVCGGTGCTSSNSAAIIEALESEIKKHGLENEVKVVRT
                     GCFGLCARGPIMVVYPEGSYYSLVKPEDVPEIVEEHLLKGRIVKRLLFEEFTMDDIKS
                     LKETDFYKKQHRVALRNCGVIDPENINEYIAYDGYAALAKCLSEYTPAQVIDIISESG
                     LRGRGGGGFPTGKKWSFTAAQKSEQKYVVCNADEGDPGAFMDRSVLEGDPHCIIEAMA
                     ICGYATGASEGYIYVRAEYPIAVKRLEIAIGQAREYGLLGKNIFDSGFDFDLHIRLGA
                     GAFVCGEETALMTSIEGNRGEPRPRPPYPAVKGLFGKPTTENKVETFANVAQIILNGA
                     DWFASMGTERSKGTKVFALGGKIKNTGLVEIPMGTTLREIVEEIGGGIPG"
BASE COUNT          425 a          362 c          439 g          304 t
ORIGIN      
        1 tatagaagag cttatggcaa taagagataa aaccagaaaa aatatgacgg tgcgcgagga
       61 taccggggac tcaaaaatca gggtggtcgt cggtatggca acctgcggca tagccgccgg
      121 agcaaggccc gttctcaatg cgtttgtcga tgagattgca aaaagagacg taaagggcgt
      181 ttccgtgcag cagaccggct gtataggaat gtgtcagtac gagccgatag ttgaggtgac
      241 cgagcccggc aaggaaaagg tcacgtacgt taagatgacg cccgaaaaag cagtaagagt
      301 tgtaaacgac catatcgtaa acggaaaccc cgttgcggaa ttcacggtcg gagccattaa
      361 agattaagga ggttaaacga tgtatcgttc acatgtgctc gtttgcggcg gtaccggctg
      421 tacctcttca aacagtgccg ctatcattga ggcgcttgaa tccgaaatta aaaagcacgg
      481 cctcgaaaac gaggtcaagg ttgtaagaac cggctgcttc ggactttgcg cgcgcggccc
      541 gataatggtg gtttaccccg agggtagcta ctattcgctt gtaaagcccg aggatgttcc
      601 cgaaatagtt gaggagcatc tcttaaaggg cagaatagtt aagaggctgc tgttcgagga
      661 gttcaccatg gacgacataa agtcgctcaa ggagacggat ttctacaaaa aacagcaccg
      721 cgttgcgctt cgcaactgcg gtgttataga ccccgaaaac ataaacgaat atatagcgta
      781 tgacggctat gcggctctcg caaagtgcct ctccgaatac actcccgcgc aggttataga
      841 cattatctcc gagtcgggac tcaggggcag aggcggcggc ggcttcccca caggtaaaaa
      901 gtggagcttc accgcagcgc agaaatcgga gcaaaaatac gttgtctgca acgccgacga
      961 gggcgacccc ggcgcgttta tggaccgttc cgtgctcgag ggcgatccgc actgcataat
     1021 agaggctatg gccatctgcg gttatgccac cggtgcgagc gagggctaca tatacgtaag
     1081 agcagaatat ccgatagcgg tcaagcggct tgaaatagca atcggtcagg cgagagagta
     1141 cggcctgctc ggcaaaaaca tattcgacag cggatttgat tttgaccttc atatacgcct
     1201 cggagcgggc gcgtttgtat gcggcgagga aaccgcactt atgacctcca tcgagggcaa
     1261 ccgcggagag ccgagaccga gacccccgta tcctgcggtc aagggacttt tcggaaagcc
     1321 gaccacggaa aacaaggtcg agacttttgc aaatgtcgct cagataatac ttaacggagc
     1381 ggattggttc gcctccatgg gtaccgagcg ttcaaaggga acaaaggtat tcgctctcgg
     1441 cggcaaaatc aaaaatacgg gacttgtaga aataccaatg ggtacaactc tccgcgaaat
     1501 agttgaggaa ataggcggcg gcatacccgg
//
LOCUS       DBMDVO010000360         1529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39252_length_1529_cov_1.282913, whole genome shotgun sequence.
ACCESSION   DBMDVO010000360 DBMDVO010000000
VERSION     DBMDVO010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39252_length_1529_cov_1.282913"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(247..924)
                     /gene="csn2"
                     /locus_tag="ACZPE2_05570"
     CDS             complement(247..924)
                     /gene="csn2"
                     /locus_tag="ACZPE2_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HIR1319351.1"
                     /translation="MRAKFSYIDDCIKIGEGIFPVLVIENKKLYRGVLSSFLNSCEED
                     YFVFSEDFKPFEFSKDGCFISEPIFVDMNSRKLLGKLDGYMQQTANDEFAEDTTEVKA
                     AIARLADKLKAFCDFDCEYSDETDTSAIIKLMGFRFSAESSSLLECFVNYLKLSAKYL
                     KTKVFVAANICLYFSPDEISELLKALALEHINFLMLENSEPQRLCDGEKLYVVDNDLC
                     VIDDGDT"
     gene            complement(921..1226)
                     /gene="cas2"
                     /locus_tag="ACZPE2_05575"
     CDS             complement(921..1226)
                     /gene="cas2"
                     /locus_tag="ACZPE2_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIR1319352.1"
                     /translation="MRVIVFFDLPTTTKSERREYAKFRKFLIKSGFMMMQESVYSKIA
                     LNQNVATGICDSVKKQKPPSGLVQMLTVTEKQFAKMEMIVGKSHSSVVDSDERLLIL"
     gene            complement(1231..>1529)
                     /locus_tag="ACZPE2_05580"
     CDS             complement(1231..>1529)
                     /locus_tag="ACZPE2_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR1319353.1"
                     /translation="LTQWGLHHDNVFNHFNLSSDLMEPFRPIVDFCVKSMMPKEFGKE
                     QKSTLIGLLNKEFVVDGKANTLMNVIKLYVKSVFAAIEQNDASLVKLAEVKYEL"
BASE COUNT          457 a          364 c          258 g          450 t
ORIGIN      
        1 atgcgccgag caacgtttca gtaccttatg aaattacact actctcaaac tgtcaatcaa
       61 agtataagcg tgtttatact agtttcagta ccttatgaaa ttacactact ctcaaacttt
      121 gtataatcat attggaaatt gtaacaggtt tcagtacctt atgaaattac actactctca
      181 aacctcaaat aaactgcgcc actacaatgc acagggtaat aacacaaggt acatttatta
      241 tactatctaa gtatctccgt cgtcaatgac gcaaaggtca ttgtcgacaa catacagttt
      301 ttcgccgtcg cagagccttt gcggctccga attctcaagc attaaaaaat tgatatgctc
      361 aagagctaaa gcctttaaca gctctgatat ttcatccggc gaaaagtaaa gacaaatatt
      421 ggcggcaaca aaaaccttcg tcttaagata ctttgccgat aatttcaaat aatttacaaa
      481 gcattccaag agcgaagaag actccgccga aaacctgaag cccattagct ttattattgc
      541 agaggtgtcc gtttcatcgg aatattcaca atcgaaatcg caaaacgctt taagcttatc
      601 ggcaagcctt gcgattgccg cctttacctc ggttgtatcc tcggcaaact cgtcatttgc
      661 ggtctgctgc atatagccgt caagcttgcc gaggagcttt ctgctgttca tatcgacaaa
      721 aatcggttcg gatataaagc acccgtcctt ggaaaactca aacggtttaa aatcctcgga
      781 aaagacaaaa taatcttcct cacacgaatt taagaatgac gacagcacgc ctctgtaaag
      841 ctttttgttt tctattacga gaaccggaaa tattccctcg cctattttta tgcaatcatc
      901 aatatacgaa aacttagccc tcataatatc aacagccttt cgtccgaatc cacaacactg
      961 ctgtggcttt tgccgacaat catttccatt ttggcgaact gtttttcggt aacggtaagc
     1021 atttgcacaa gacccgacgg cggtttctgc tttttcaccg agtcgcaaat gcccgttgcg
     1081 acattttggt taagcgcaat ttttgaataa accgattcct gcatcatcat aaatccgctt
     1141 ttaatcaaga atttacgaaa ttttgcatat tcgcgccgtt cgctcttggt agttgtcggc
     1201 aaatcaaaaa atacaattac tctcataaat ctataactca tacttcactt ccgcaagctt
     1261 cacaagcgaa gcgtcatttt gctctattgc ggcaaatacg ctttttacat agagttttat
     1321 tacattcata agcgtattag ccttgccgtc cactacgaat tccttattca gtaagcctat
     1381 caaagtgctc ttctgctcct tgccgaattc cttgggcatc atggatttta cgcaaaaatc
     1441 gactatcggg cgaaacggct ccattaaatc gctgctcagg ttaaaatggt taaacacatt
     1501 atcatgatgc aacccccact gcgtgagat
//
LOCUS       DBMDVO010000361         1528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39283_length_1528_cov_1.304835, whole genome shotgun sequence.
ACCESSION   DBMDVO010000361 DBMDVO010000000
VERSION     DBMDVO010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39283_length_1528_cov_1.304835"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="ACZPE2_05585"
     CDS             complement(<1..371)
                     /locus_tag="ACZPE2_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319354.1"
                     /translation="MEWSNIRMSKAEFERLQKEYAEAAIRTAEKAKLSYDADEPKEEI
                     PKTENIAAAISRLPTPAEENVNETIKPDSAKITTVQVKITEAESEITASDTEDENKES
                     EEAETNIPVNEGKSAVNADKS"
     gene            complement(386..727)
                     /locus_tag="ACZPE2_05590"
     CDS             complement(386..727)
                     /locus_tag="ACZPE2_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319355.1"
                     /translation="MSEIDDILKAILSDNKNDNATSEETSGTENEEKFTTDTDSDDIF
                     GGINIDMFMKIGELMSKMSEQDRNTGLLLALKPHLREENRAKIDTAVKLFRLISLLPY
                     LKDSGLFDNLL"
     gene            complement(743..973)
                     /locus_tag="ACZPE2_05595"
     CDS             complement(743..973)
                     /locus_tag="ACZPE2_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319356.1"
                     /translation="MNNNVDSMLEAVSSKLGITPQRLKEAMQKGDISIALEKMPEKDA
                     KKLKSILGNPELIKKMMKSAQVQELKKSMKNQ"
     gene            1258..>1528
                     /locus_tag="ACZPE2_05600"
     CDS             1258..>1528
                     /locus_tag="ACZPE2_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1319357.1"
                     /translation="MKIVVVSDSHKEFHKLNSVVENNLDADAFIHLGDGEHEFNDVRN
                     LHPEKSFLFVKGNCDFADNKTIRIANAKGIKILCVHGHEHHVHQGL"
BASE COUNT          366 a          327 c          287 g          548 t
ORIGIN      
        1 tcggatttat ctgcgtttac ggcggatttt ccttcgttta cgggaatatt tgtttcggct
       61 tcctcgcttt ctttattttc atcttcagtg tcagaagccg taatctcgct ttcggcttcg
      121 gtaattttta cctgcactgt tgtgattttt gccgaatcgg gctttattgt ttcgttcacg
      181 ttttcttctg ccggcgttgg aaggcgtgat attgccgcag cgatattttc ggttttcgga
      241 atttcttctt tcggttcatc cgcatcgtaa ctgagctttg ctttttcggc tgttcttatt
      301 gccgcctcgg catattcttt ctgcaaacgc tcaaattcgg cttttgacat tcttatattg
      361 ctccattcca taaatttact ccttttcata gtagattatc aaacagaccg ctgtctttaa
      421 gatagggaag cagtgaaatc agcctgaaca gctttaccgc cgtatctatt tttgccctgt
      481 tttcttctct taagtgaggc ttcagagcaa gcagcagacc tgtatttctg tcctgctcgc
      541 tcatctttga cattaactcg cctatcttca taaacatatc tatgtttatt ccgccgaaaa
      601 tatcatcgct gtcggtatcg gtcgtgaatt tttcctcgtt ttccgtaccg cttgtttctt
      661 cagaggttgc attgtcattt ttattatcgg acagtatcgc tttaagaata tcatctatct
      721 cactcatact gccctccgtc cattactgat ttttcataga ttttttcagc tcctgcacct
      781 gtgcggattt catcattttt ttgataagct ccgggtttcc gagtatgctt tttagctttt
      841 ttgcgtcttt ttccggcatt ttttcgagtg ctatcgaaat atcgcccttc tgcatcgctt
      901 ctttaagacg ctgaggcgtg atgccgagtt tactactgac cgcctcaagc attgaatcaa
      961 cgttgttatt cataactgcc tcctgaaatt atattgtcaa tgcggaaaat atattcccgt
     1021 taagactgcc ctaaaaccgt atcctatgaa ctttttgaca aaatcaacga taaaaggata
     1081 cttacgttat attcagttca tatttttttg taaaaatata caaaaggttg tttttgtttt
     1141 gtgtagaatt tctaaaaata cggatactaa tcttttcttt ttacacatta tgtgttataa
     1201 taacctagtg ggcaataaaa gccgtatctg gcttgcccga ctgaaaggat agaaattatg
     1261 aaaattgtag tagtatccga ttcgcacaag gaatttcata agctgaattc ggtagttgag
     1321 aataatcttg acgccgacgc ttttatacat ctgggtgacg gcgaacacga attcaacgat
     1381 gtaagaaatc ttcaccccga aaagagcttt ttattcgtaa agggaaactg tgactttgcc
     1441 gacaacaaaa cgataaggat tgcaaatgca aagggcatca agatactttg cgtgcacggc
     1501 cacgagcatc atgtgcatca gggacttg
//
LOCUS       DBMDVO010000362         1528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39304_length_1528_cov_0.795375, whole genome shotgun sequence.
ACCESSION   DBMDVO010000362 DBMDVO010000000
VERSION     DBMDVO010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39304_length_1528_cov_0.795375"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="ACZPE2_05605"
     CDS             complement(<1..1513)
                     /locus_tag="ACZPE2_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR1319358.1"
                     /translation="MIYLSCGSSSDSLASMGFDGAYAYNWGNQGYKLDVNKNSILNSQ
                     KASSKMYTVPTISVGFNSIPWHGIRYPMMSVEDFKTAHTWIRDEYLPTYAAKGTWQEK
                     LSMISTWNEYGEGTYIMPSKGNGGFGYLDALREVYTDEKVDKSLDLYPTDAQRTRINR
                     LYPQYRHLLRKTGYYDFSVDTSNLESVYTITYTENKAMGVGNTKTYEFTPEGLKGMVD
                     GDTLIIAAALNVPAEANQVKVTIDVPKGTYVELYYVTDSDKNWTGSKSSGFTADKEGL
                     NEYLVDMSQKSDWKGTITQFRVDPGQTEPGMGDPEKNYFRLVSVEFLKAPSKGSRNIF
                     INGKKYELQLYPEAAANGDYLVSFDPAVALNYLLNSFYTWDRDAGVLTIEANKHTVVY
                     TTGSDKYTVDGKTKDLGYKMYLQDGLPMIPIVKFCEDVGYKASVNENKEAVIETNQKE
                     YFDMIAKREPAKWEFNTPGDNESWSSGHMNLFTVDGYMRATSITDSRDPIITFN"
BASE COUNT          335 a          364 c          424 g          405 t
ORIGIN      
        1 cgttgaacgt gataatcggg tcgcggctgt cggtaatgga ggtggctctc atataaccgt
       61 ctaccgtaaa gaggttcata tgtcccgaag accagctttc gttatcaccc ggcgtattga
      121 attcccactt tgcgggttcg cgcttggcga tcatatcgaa gtattctttt tggttggttt
      181 cgataacggc ttccttgttt tcgttaaccg atgccttgta gccgacgtct tcgcagaact
      241 taacaatcgg aatcatcggc agaccgtcct ggaggtacat cttgtagccg agatccttgg
      301 tcttgccgtc aacggtgtac ttatccgagc cggtggtata aacgacggta tgcttattgg
      361 cttcaatggt gagaacaccg gcatctctgt cccaggtata gaacgaattg agtaagtagt
      421 tgagtgcaac ggcagggtcg aaggagacaa ggtaatcgcc gtttgcggcg gcttccggat
      481 agagctgaag ctcgtatttc ttgccgttga tgaagatgtt gcggctgccc ttgctgggag
      541 cttttaagaa ttcaacgctg acaagacgga aatagttctt ttcgggatcg cccatgcccg
      601 gctcggtctg accggggtca acacggaact gggtgatggt acccttccag tcggacttct
      661 ggctcatatc aaccaaatat tcgtttaagc cttccttgtc agccgtaaag ccggagctct
      721 tcgaaccggt ccagttttta tcggaatcgg tcacatagta gagctcgaca tacgtgccct
      781 tcggaacatc gatggtcacc ttgacctgat ttgcttcagc cggaacattg agagcagcag
      841 cgataataag cgtatcgccg tcaaccatac cctttaagcc ctccggcgta aattcatagg
      901 tcttggtatt gccgacgccc atagccttgt tttcggtata cgtgatggta tagacgcttt
      961 caaggttgga ggtatccact gagaaatcgt agtaaccggt cttgcgaaga agatgtctgt
     1021 actgcggata gaggcggttg atgcgtgttc tctgcgcgtc ggtcggatag aggtcgagag
     1081 acttgtcaac cttttcatcg gtatacactt cgcgcaatgc gtcgagatag ccgaagccgc
     1141 cgttaccctt ggacggcatg atgtacgtac cttcgccgta ctcgttccag gtggagatca
     1201 tggagagctt ttcctgccat gtcccctttg cagcataggt cgggaggtat tcgtcacgga
     1261 tccatgtgtg tgcggtcttg aaatcttcaa ccgacatcat cgggtagcgg atgccgtgcc
     1321 acggaatgct gttgaagccg acagagatgg tcggaacggt gtacatcttg gaggaagcct
     1381 tttggctgtt gaggatagag ttcttgttga cgtcgagctt gtagccctgg ttgccccagt
     1441 tataggcata tgcaccgtca aagcccatgc ttgcaagaga atcggaggag gagccgcagg
     1501 acaggtagat cataccgtca tagccgag
//
LOCUS       DBMDVO010000363         1525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39383_length_1525_cov_1.191011, whole genome shotgun sequence.
ACCESSION   DBMDVO010000363 DBMDVO010000000
VERSION     DBMDVO010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39383_length_1525_cov_1.191011"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="ACZPE2_05610"
     CDS             <1..1099
                     /locus_tag="ACZPE2_05610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1319359.1"
                     /translation="NAVINDYQKEIESLQKNIDEASESIETQTKTVNDTYNLLKERLR
                     AAYMAGESSTLEVLLTASDYEGFLTRLELLSKTTKHDRQLMKSLQDDIAKLNDTKELL
                     SSNQQEVKAKQTAVESEKADIVSSKTQVQSLYNTVDSKQSTLEKQVAQRNAYISSLSA
                     GSKELENENKKIQAAKDSYDKKLAELAKNASQGTGELPKPGGNTGGNAGGNTGGNTGG
                     NTGGYTVSPKGMICPLQYPNVTISAGWYGYPNHKGIDFTTRGATGNTYGKEIRAAADG
                     KVLSAEYHSSWGNNVFIDHGNGVCTRYAHCSVMKVSKGQTVKQGQVIALVGNTGNVSP
                     APTPANPHAGAHLHFEVWINGTRVNPAPYLP"
     gene            1143..>1525
                     /locus_tag="ACZPE2_05615"
     CDS             1143..>1525
                     /locus_tag="ACZPE2_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319360.1"
                     /translation="MKHQKKAFIRIISVTLITAMLFSVTAFSGSASSVQDQIDELESK
                     INANNNKISDNKASVNDLLNQISDMQAQLDVYNAKITDLNKQIAAKDEIINNYQAEID
                     RLQAEIDAANIQIETQTAAVNVTYDV"
BASE COUNT          498 a          328 c          370 g          329 t
ORIGIN      
        1 aaacgccgtt ataaacgatt atcaaaaaga aatagaatca cttcaaaaga acatagacga
       61 ggcaagcgag agcatagaaa ctcagacaaa gaccgtaaac gatacatata accttttaaa
      121 ggagagactt cgcgcggcat atatggcagg cgaaagttcc actcttgagg tgcttcttac
      181 ggcaagtgat tatgagggct tcctcacaag gcttgagctt ttaagtaaaa ccacaaaaca
      241 tgacaggcag cttatgaaat ctttgcagga tgatattgca aagcttaacg acacaaagga
      301 gcttctgtcg tcaaatcagc aggaagtaaa ggctaagcag accgccgtag agtcggaaaa
      361 ggcggatatt gtttcgtcta aaacgcaggt gcagtcgctt tacaataccg tagattcaaa
      421 gcagtctacc cttgaaaagc aggttgcaca gcgaaatgca tatatctcgt ctctcagtgc
      481 aggctccaag gaattagaaa acgagaataa aaaaatacag gctgcgaaag acagctacga
      541 taaaaagctt gcggaactgg cgaaaaatgc gtcgcagggc accggcgaat tgccaaagcc
      601 cggcggcaat acaggtggaa acgcgggcgg caatacggga ggaaataccg gtggaaatac
      661 aggcggttat acggtgtctc ccaagggtat gatttgtccg cttcagtatc caaacgtaac
      721 catatcggca ggctggtacg gctacccgaa tcacaagggt attgatttta ccacaagggg
      781 tgcaaccggc aatacatacg gtaaggagat cagagccgcg gctgacggta aggttctttc
      841 cgccgaatat cattcgagct ggggcaataa cgtctttatt gaccacggca acggcgtttg
      901 cacaagatac gctcattgca gcgttatgaa ggtaagcaaa gggcaaacgg taaagcaggg
      961 gcaggttata gctcttgtag gcaatacggg taacgtaagt cccgcgccga cgccggcaaa
     1021 tccgcacgcc ggggcgcatc tgcactttga ggtctggata aacggcacaa gggtaaatcc
     1081 cgcgccgtat cttccgtaat ttttaaaggg gtaaatccgg tattaaagga gttcttttta
     1141 ttttgaagca tcagaagaaa gcatttatac gcattatttc cgttacatta ataacagcta
     1201 tgttgttttc cgtgacggcg ttttcgggct cggcttcgtc cgtgcaggat cagatagacg
     1261 agcttgaaag caaaataaat gccaataata ataaaatcag cgacaataaa gcatccgtaa
     1321 acgatttgct caatcagatt tccgatatgc aggctcagct tgatgtttac aacgccaaga
     1381 taaccgacct taacaagcaa atagcggcaa aggatgaaat tatcaacaac tatcaggcgg
     1441 aaatcgacag acttcaagcc gaaatagacg ccgcaaatat tcaaatagaa acacaaacag
     1501 ccgctgtcaa tgtaacatac gatgt
//
LOCUS       DBMDVO010000364         1522 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39484_length_1522_cov_1.130190, whole genome shotgun sequence.
ACCESSION   DBMDVO010000364 DBMDVO010000000
VERSION     DBMDVO010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39484_length_1522_cov_1.130190"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="ACZPE2_05620"
     CDS             complement(<1..1417)
                     /locus_tag="ACZPE2_05620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319361.1"
                     /translation="MNISSINKKLKKTFGGKFSASKENGSIFVRGKSSDWGEIVAACQ
                     AAAKKFSTTHIVNDIVYTGEQPAPTRLPSLKDDFLEGRTPDVLVIGGGISGASIAREL
                     TKWKLDVLLVDKEADLALQASGRNDGEVHPGIDLGKGSLKHKYIRRANHMYADVCREL
                     DVPFKQVGQYVLFRNAALKPLIGLYAFWRKHHDGIEDTEIISGKELMRREPNLTPETK
                     FAMYNPSAGCVCPYGLTIAYAENAVQNGAQVSLNTAVLSMEVSEHNIISVTTNRGVIH
                     PKIVINAAGVFAEDIAAMADDRFFSIHPRRGTNSILDQKAGAYMHSVASVKDISVHGK
                     THSKGGGILHTVHDNLLVGPDAVETVEKENTETRAESIKTVFDKQTQTMPALSKRDII
                     TYFTGVRAPTFEEDFILEPGRRTRNLIHVAGIQSPGLTTAPAVAVDMAELAVDMLGKT
                     ETVEKNNNYNPIRKGVPVLREM"
BASE COUNT          331 a          390 c          388 g          413 t
ORIGIN      
        1 ccatttcgcg aagcacggga acgcccttgc gaataggatt gtaattgttg tttttttcaa
       61 ccgtctcggt cttgccgagc atatcgaccg caagctccgc catatcgacg gcaaccgcgg
      121 gagcggttgt aagtccgggg gattgaatac cggctacgtg aatcaaattc cttgttcttc
      181 tgccgggttc aagtataaaa tcctcctcaa aggtaggagc gcgaacgccc gtgaaataag
      241 tgattatatc acgttttgac agcgccggca ttgtttgagt ctgcttgtcg aagacggttt
      301 taatgctctc ggcacgtgtt tccgtgtttt ccttttcaac cgtttctacg gcgtcgggtc
      361 cgacgaggag attatcgtgg acggtgtgca atatgccgcc gccctttgaa tgggttttac
      421 cgtgaacgct tatgtccttt acggaggcta ccgagtgcat atatgcgcct gctttttggt
      481 caagaatcga gttggttcct cgacgcggat gaatcgagaa aaatctgtca tccgccatgg
      541 cggcaatatc ctcggcaaaa acgcctgcgg cgttgataac gattttcgga tgaataacgc
      601 ctctgttggt ggtaacgctt atgatgttat gctccgacac ctccatggag agcacggcgg
      661 tgttaaggct tacctgcgcg ccgttttgaa cggcgttttc ggcataggct atggtgagtc
      721 cgtacgggca aacacagcct gcgctcggat tatacattgc gaattttgtt tccggtgtga
      781 ggttcggctc gcggcgcata agctccttgc ccgagattat ctcggtgtcc tctatgccgt
      841 cgtgatgctt ccgccaaaaa gcatacagac cgataagcgg cttcagcgcg gcgttgcgga
      901 aaagcacata ttgacctacc tgcttaaacg gaacgtcaag ctcgcggcaa acgtctgcat
      961 acatatggtt tgcgcggcga atgtacttgt gcttgagcga gcctttgccg aggtcgattc
     1021 cggggtgcac ctcgccgtcg ttgcgtcccg acgcctgaag tgcaaggtct gcctccttgt
     1081 ctacaagtaa aacgtcaagc ttccacttgg tcagctctct tgctatcgaa gcacctgaaa
     1141 tgccgccgcc gataacaagc acgtcgggtg ttctgccctc gagaaaatca tccttgagag
     1201 acggcagacg cgtcggagcg ggctgctcgc ccgtgtaaac aatatcgttg acaatatgcg
     1261 ttgtgctgaa tttttttgcc gccgcttggc aagccgcgac gatttcgccc caatcggagc
     1321 ttttgcctcg aacaaaaatc gagccgttct cctttgaagc actgaatttt ccgccgaaag
     1381 tctttttcaa ttttttgtta attgatgata tgttcataac ttcaagcctc ttttaatcag
     1441 ccttgcagtc ggtcacacgc ccgactgtgc ataaaaaata tgtctgttaa ctgaccgact
     1501 cataagcaaa ttataattcc tc
//
LOCUS       DBMDVO010000365         1517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39654_length_1517_cov_1.326977, whole genome shotgun sequence.
ACCESSION   DBMDVO010000365 DBMDVO010000000
VERSION     DBMDVO010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39654_length_1517_cov_1.326977"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            187..1227
                     /locus_tag="ACZPE2_05625"
     CDS             187..1227
                     /locus_tag="ACZPE2_05625"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1319362.1"
                     /translation="MARSLKKLIAAVFCLVLLFELSSCAVNTKSAEQTGFSMGSLVGI
                     KLYGKNSEALENANLLAEVVKTDERISKNYEGSDIYRVNSSGEHGETVSSETYDELRT
                     VCDVYSDSHGKAAVLSGALTELWGFDTDDFRLPSEDEILSAKEKCGDDKLIFDDGKKV
                     YIPDGAKLNLGSAGKGIALDEAVKRIDEINSQGGKITGAVISVGGSVATYGLPKKGKS
                     WNVGIRDPYKTENDYFAVLSLTDAFISTSGDYEKRFTAEDGKTYFHILDLTTGYPVQT
                     ELTSVTIKAPTGLLSDALSTLCFILGKEESLPILEKYNADAVFVYKDRTVFATDGIKP
                     YLKITNGDYSLV"
     gene            1229..>1517
                     /locus_tag="ACZPE2_05630"
     CDS             1229..>1517
                     /locus_tag="ACZPE2_05630"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1319363.1"
                     /translation="MERKLLGKHGAALIIITVMVIAVLTAYKYTRAKTSLTAVVTSDG
                     VTVLTLSLDEIAEKREFTLDNGIVIAAENGEIWVESSPCKDKICMRTGKLCR"
BASE COUNT          431 a          305 c          399 g          382 t
ORIGIN      
        1 gcggttttta cgggggagca tcgctttgag ccttgcttcc cctgaatttt tataggcttg
       61 agacaaaaag ggaagtttta cacggcaaac tttgttaatt ttcaaacttt gcttgaacta
      121 acccttgaaa tattgtatat ttaattgtag tgtcgtcgaa ttaggcagtt gtgaggtgaa
      181 acaagcttgg caaggtcgtt aaaaaaactt attgccgctg tgttttgcct tgttttactg
      241 tttgagctgt cttcgtgcgc ggtaaacaca aaatcagcgg agcagacggg attttccatg
      301 gggtcccttg taggcattaa gctttacggg aagaactccg aggcgttgga aaacgcaaac
      361 cttttggctg aagtggttaa aacagacgag cgcatttcaa aaaattatga gggcagcgat
      421 atatacagag taaattcctc gggcgagcac ggagaaacgg tgagctctga gacatatgat
      481 gagcttcgca ccgtatgcga tgtgtattcc gattcccacg gcaaggctgc cgttttgtcc
      541 ggagcgctga ctgagctttg gggttttgat accgacgatt tccgcctccc gagcgaagat
      601 gaaattttat ccgcaaaaga aaaatgcggt gacgataaat tgattttcga cgacggcaaa
      661 aaggtttata tccccgacgg cgcaaagctc aatctcggct ccgcaggcaa aggaatagca
      721 cttgacgagg cggttaagag aatagacgaa ataaattcgc agggcggcaa aataacgggg
      781 gccgtaattt ccgtaggcgg cagcgtagcg acatacggct taccgaaaaa aggcaaaagc
      841 tggaatgtag gaatacgcga cccgtacaaa acggaaaacg attattttgc ggtgctgtcg
      901 cttacagatg cttttatatc aacctcgggc gattatgaaa agagatttac cgccgaggac
      961 ggaaaaacat attttcacat tttggatttg accaccggct atccggtgca aacggagctt
     1021 acaagtgtta cgataaaggc tcctacgggg cttttgagcg acgctctctc aacgctctgt
     1081 tttattctcg gaaaggaaga gtctctgccg atactcgaaa agtacaatgc ggacgcggtg
     1141 tttgtctata aggacagaac cgtctttgcg acggacggca taaagccgta tttaaaaata
     1201 acaaacggcg attactctct tgtctgatat ggaaagaaag cttttaggaa agcacggagc
     1261 ggcgcttata ataattacgg ttatggtaat tgcggtgctt acggcgtata aatacacaag
     1321 ggcgaaaaca tcactcactg ccgttgtcac gtcggacggt gtgaccgttt tgacgctgtc
     1381 tcttgatgag attgcggaaa agcgagagtt tacgctcgac aacggtattg taatagccgc
     1441 cgaaaacggc gaaatatggg tagaaagctc tccgtgtaaa gacaagatat gtatgagaac
     1501 gggtaagctg tgccgcg
//
LOCUS       DBMDVO010000366         1517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39657_length_1517_cov_1.250706, whole genome shotgun sequence.
ACCESSION   DBMDVO010000366 DBMDVO010000000
VERSION     DBMDVO010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39657_length_1517_cov_1.250706"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="ACZPE2_05635"
     CDS             <1..1153
                     /locus_tag="ACZPE2_05635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR1319364.1"
                     /translation="IINEHGRFALHLMPSGVCYDNVTCVIGNGVALDINKFCSELEDV
                     KKAGVNPKLLVSDRAQILMPYHILLDTYEEEILGKNAFGSTKSGIAPFFSDKYAKIGI
                     QCNELFDDEVLKAKLHNIVTLKNLILEHVYHKPLLDEDELYNTCMEYKKKIAPYICDT
                     HAFIRDALKQGKNILLEGQLGTLKDPDFGIYPMVTSSSTLAGYGSVGAGIPPYLISEV
                     VAVTKAYSSAVGAGEFVSEILDEDEAQELRIHGGDKGEFGATTGRPRRVGWFDCVATR
                     YGVECQGATKIAMTALDCLSYLDEIKVCTAYKIGDDITKDFPNTSLLKKAKPVFTTLK
                     GWHCNIKGIRNFSELPREAQEYVEFIEKELGHKINMVSNGPEREAIIYR"
     gene            1168..>1517
                     /locus_tag="ACZPE2_05640"
     CDS             1168..>1517
                     /locus_tag="ACZPE2_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR1319365.1"
                     /translation="MEKILVLDFGGQYNQLIARRVRDNNVYAEILPYTAPLSEIKEGN
                     YKGIIFTGGPQSAYGEGAPSIDAGVFELGVPIMGICYGAQLMAYMLGGKIDSAPTSEY
                     GRADVVIDDKDGIL"
BASE COUNT          418 a          348 c          391 g          360 t
ORIGIN      
        1 cattataaac gaacacggca gatttgcatt gcatcttatg cccagcggtg tttgctacga
       61 caatgttacc tgtgtcatcg gaaacggcgt cgctcttgat ataaacaagt tctgctcgga
      121 acttgaggac gtgaaaaagg caggtgtaaa tccgaagctt ctcgtgtcag accgtgctca
      181 gatacttatg ccgtatcata ttcttctcga tacctatgaa gaggagatac tcggcaaaaa
      241 cgcgttcggc tccacaaaga gcggtatcgc tccgttcttc agcgacaagt acgcaaaaat
      301 aggcatccag tgcaacgagc tgtttgacga tgaagtgtta aaggcaaagc ttcacaacat
      361 agtgactctc aaaaatctca ttcttgagca cgtttatcat aagccgctgc ttgacgagga
      421 cgagctttac aacacctgta tggaatacaa gaaaaagata gctccgtaca tctgcgatac
      481 gcacgcattt atacgcgacg ccttaaagca gggcaaaaac attctcctcg agggtcagct
      541 cggcacgctt aaggatcccg atttcggtat atatcccatg gttacgtcgt cctcgacttt
      601 ggcaggctac ggctctgtcg gcgcgggaat tcccccgtat ctcataagtg aagtagtagc
      661 cgttaccaag gcatattctt cagcggtagg tgcaggtgaa tttgtttcgg aaattcttga
      721 cgaggacgag gctcaggagc tcagaattca cggcggcgac aagggtgagt tcggcgctac
      781 caccggcaga ccgagaagag tcggctggtt tgactgcgtt gcgacacgtt acggcgttga
      841 gtgccagggt gccacaaaga ttgccatgac cgctcttgac tgtctttcgt accttgacga
      901 aatcaaggtt tgcacagctt ataaaatagg tgatgacatc acaaaggatt tcccgaacac
      961 gtcgctcctt aaaaaggcaa agccggtatt taccacatta aagggttggc attgcaatat
     1021 aaagggcatt cgcaatttct cggagcttcc gcgtgaggca caggaatatg tcgaattcat
     1081 tgaaaaggaa ctcggccaca agataaatat ggtatctaac ggacctgaga gggaagctat
     1141 aatttacaga taattaagag gatatttatg gagaaaatac ttgtactcga tttcggcggt
     1201 cagtataatc agctgattgc gcggcgtgta cgcgacaaca atgtctatgc ggaaatactg
     1261 ccgtataccg caccgctcag cgaaatcaaa gagggcaatt acaaaggtat tatttttacc
     1321 ggcggaccac agagcgcata cggcgaaggt gcaccgagca tcgatgccgg cgtgttcgag
     1381 ctgggcgttc cgattatggg aatctgctac ggtgcgcagc tgatggcata catgctgggc
     1441 ggcaaaatcg attccgctcc gaccagtgaa tacggtcgtg cggacgtggt aatcgatgat
     1501 aaggacggca tcctgaa
//
LOCUS       DBMDVO010000367         1517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39671_length_1517_cov_0.884887, whole genome shotgun sequence.
ACCESSION   DBMDVO010000367 DBMDVO010000000
VERSION     DBMDVO010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39671_length_1517_cov_0.884887"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="ACZPE2_05645"
     CDS             <1..>1517
                     /locus_tag="ACZPE2_05645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1319366.1"
                     /translation="CAAEQNVGVSSPEILNVKEDGRSPELVYLEKYLCGEESEPYKEE
                     CDNIVVCAAGNRADEADFVACEIKKLLRCNPTLRCRNIAVIERTAGTYDKELSESFKK
                     YGVPMFEDKRRPIGIQPVMVFVKCLLALCAEGFSTESFMRLLKTGLTALSGDEISELE
                     NYAFLWNIDGSKWRRDFTENPDGLGFDVTEKSQEKLIRLNDIRKRSVLPVLAFKKDFE
                     EADGEKKSRLIYEFLIKNGITERLREKIEYLVSSGDTVLCEEQDTVWATLMDMLGKMA
                     SAIGETVITAKRYSELFNILLSAVTLGSLPQELDSVTVGAADRIRVSDCKVAFVIGAD
                     SGVFPLDPSTEGLLSDSERKVMGALGVKLAETAEYKAVDEKLTAYRAVTSPSEKLYVT
                     YSLSNFDGTSMHPSEIVNNIVTAFPTVPKLDTALSEPLYSIESDASAFKVLATGYCEN
                     TELVVTLKKYFGEKNEYSSRIRSLDRIASGDKMKIRDSKNAISLFGKSMYVSASRV"
BASE COUNT          423 a          312 c          392 g          390 t
ORIGIN      
        1 tgtgccgcag agcaaaatgt cggcgtttcg tctcccgaaa tactaaatgt taaagaggac
       61 ggcagaagcc ccgagcttgt ttatcttgaa aagtatttgt gcggcgaaga atccgaacca
      121 tataaagaag aatgcgataa catagttgtg tgtgcggcgg gaaacagagc tgatgaagcg
      181 gattttgttg cgtgtgaaat taaaaagctg ttaaggtgta atcccacgct tcgatgccgc
      241 aatatagccg ttatagagcg tactgcagga acatacgaca aggaactttc cgagagcttt
      301 aaaaaatacg gcgttccgat gtttgaggat aagcgccggc ctatcggtat tcagcctgta
      361 atggtattcg tcaaatgcct actggcactt tgcgccgagg gcttttctac ggaaagcttt
      421 atgaggcttt tgaaaacggg tcttaccgcc ttgtcgggtg atgaaatatc cgagctcgaa
      481 aattacgcct ttttatggaa catcgacggc tcgaaatggc gcagagattt tacggaaaat
      541 cccgacggtc tcggatttga tgtgaccgaa aaaagccaag aaaaattgat aagactgaat
      601 gacatccgca aaagatcggt tttaccggta cttgccttta aaaaggattt tgaagaggct
      661 gacggcgaaa aaaagtcgcg gctcatatat gagtttttaa tcaaaaacgg cattaccgaa
      721 agactgcgag aaaaaattga atacctcgtt tcgtctggtg ataccgttct ctgtgaggag
      781 caggatacag tttgggcaac gcttatggat atgctcggaa aaatggcgtc ggcgataggc
      841 gagacggtta taacggcaaa acggtacagt gagcttttta atatactgct gtctgccgtg
      901 acactcggct cgctgccgca ggagcttgac agcgtaactg tcggcgcagc ggacagaatc
      961 agggtttccg actgtaaggt ggcatttgtt atcggtgccg acagcggagt ttttccgctt
     1021 gacccttcaa ccgagggact tttaagcgac agcgagcgta aggtgatggg ggctttgggc
     1081 gtgaagcttg ccgaaaccgc cgaatataaa gcggttgatg agaagcttac ggcttatcgt
     1141 gccgtaacgt ctccttcgga aaagctttac gtaacctatt cgctttcaaa ttttgacggt
     1201 acttctatgc acccgtccga aatagtaaac aatattgtca cagcttttcc gacagtacct
     1261 aagcttgata cggcactttc ggagccgctg tattctatag agagcgacgc ctcggcgttt
     1321 aaagtgcttg caaccggcta ttgtgaaaat actgaacttg ttgtaacgct gaaaaaatat
     1381 ttcggcgaaa aaaacgagta ttcttcccgg ataaggtctc tcgacaggat tgcctcggga
     1441 gataaaatga aaatccgaga ttcgaaaaat gcaatttccc tattcggcaa gagtatgtat
     1501 gtttccgcgt ctcgtgt
//
LOCUS       DBMDVO010000368         1516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39691_length_1516_cov_1.424735, whole genome shotgun sequence.
ACCESSION   DBMDVO010000368 DBMDVO010000000
VERSION     DBMDVO010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39691_length_1516_cov_1.424735"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="ACZPE2_05650"
     CDS             <1..1442
                     /locus_tag="ACZPE2_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319367.1"
                     /translation="DKVMKYIDKNPQENICKMLSVAEKLTKNIFPAGNLKKMREVVRD
                     DNNVWTKYALGILNDIDRDVIKKMIMAFGFDAGYYGTKTVRRNREKLHCNVPFIILFD
                     PTSACNLHCKGCWAAEYGHKQSLTNEEMQSIVSQGKALGTHVYMLTGGEPLIRKNDII
                     ELAANNQDCVFLIYTNATLVDQKFCDDLKRVGNVALAMSIEGTEESNDWRRGEGAYNA
                     TLKAMELLKKNKCLFGISVCYTRKNDEYVTSDDFIDKMIENGSKFAFYFNYMPVGHGA
                     DKELIPTPDQREYMYRWLRKMRNGKTGKPMFVMDFQDDGEYVGGCIAAGRNYFHINSA
                     GDIEPCVFIHYSDTNIRTHTLLEALKSPLFTAYYKGQPFNDNHLRPCPMLENPELLRK
                     IVKESGAHSSDLLNKEDVDELCDKCVDFAKEWAPRAEELWNSTKHRETHTQYYRDTEE
                     GKREFGCSGNCAYCASHAAEASDSEKKAD"
BASE COUNT          453 a          383 c          367 g          313 t
ORIGIN      
        1 ttgacaaggt aatgaaatac attgacaaga atccgcagga aaacatctgc aaaatgctgt
       61 cggtggcgga aaagctcacc aaaaacatat tccccgcagg caatcttaaa aagatgaggg
      121 aagtcgtccg ggatgataat aatgtatgga ccaagtacgc cctcggcatt ttaaacgaca
      181 ttgaccgcga tgtaatcaaa aaaatgatta tggcgttcgg ctttgacgca ggctactacg
      241 gcacaaagac cgttcgcaga aatcgcgaaa agcttcactg caacgtgccg ttcataatcc
      301 tttttgaccc cacgagcgcg tgcaacctcc actgcaaggg ctgttgggct gcggaatacg
      361 gccacaagca aagccttacg aacgaagaga tgcagagcat tgtctctcag ggcaaggcgc
      421 tcggcactca tgtatatatg ctcaccggcg gcgagccgct catacgcaaa aacgacataa
      481 tagagcttgc cgcaaacaat caggattgcg tatttttaat ctatacaaac gcaacgcttg
      541 tagaccaaaa attctgcgat gaccttaaaa gagtcggcaa cgtcgccctt gccatgagca
      601 ttgagggcac ggaggagtca aacgactggc gccgcggcga gggagcttac aatgctacac
      661 tcaaggctat ggagcttctt aaaaagaaca agtgcctctt cggcatttcg gtctgctaca
      721 cgcgcaaaaa cgacgaatac gtaacgagcg acgatttcat cgacaagatg atagaaaacg
      781 gctcgaaatt cgcattttat ttcaactata tgcccgtggg tcacggtgcc gacaaggagc
      841 ttatcccgac tcccgaccag agggaatata tgtaccgctg gcttcgcaaa atgcgtaacg
      901 gcaaaacggg caagccgatg ttcgttatgg acttccagga cgacggcgaa tatgtcggcg
      961 gctgcatagc ggcaggcaga aactatttcc acataaattc cgcaggcgac atagagccgt
     1021 gcgtatttat acactattcc gatacaaata tacgcactca cacgctcctc gaggcactca
     1081 agagtccgct ctttaccgca tattacaagg gtcagccgtt taacgacaat catttaaggc
     1141 cctgtccgat gctcgaaaat cccgagcttt tacgcaaaat agtaaaggag agcggagcgc
     1201 attcctcgga ccttttaaac aaagaggatg tcgatgagct ttgcgataaa tgcgtagact
     1261 ttgcaaagga atgggcaccg cgtgccgaag agctttggaa ttccacaaag caccgcgaga
     1321 cgcacacaca gtactaccgc gacaccgaag agggcaaacg cgaattcggc tgctccggca
     1381 actgcgccta ctgtgcttcg cacgccgcag aagcttccga ttcggaaaag aaagcggact
     1441 gaaaccaaga gctttaataa agcataaaga ccggctgcag cattttgtta cagccggtct
     1501 ttcaattaag cacctt
//
LOCUS       DBMDVO010000369         1513 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39806_length_1513_cov_1.159348, whole genome shotgun sequence.
ACCESSION   DBMDVO010000369 DBMDVO010000000
VERSION     DBMDVO010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39806_length_1513_cov_1.159348"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="ACZPE2_05655"
     CDS             complement(<1..490)
                     /locus_tag="ACZPE2_05655"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIR1319368.1"
                     /translation="MLKVIKKVGNARLCDFETVHGTIHTPAFMNVATAGAIKGGLSAV
                     DLENIETQVMLCNTYHLHVRPGDDLIHDLGGLHKFTGWRGPILTDSGGFQVFSLAKLR
                     KIKEEGVSFNSHVDGRRIFMGPEESMQIQSHLGSTIAMAFDECVENPAEYSYSKQSCA
                     RTV"
     assembly_gap    517..526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<527..>1513)
                     /gene="queA"
                     /locus_tag="ACZPE2_05660"
     CDS             complement(<527..>1513)
                     /gene="queA"
                     /locus_tag="ACZPE2_05660"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1319369.1"
                     /translation="KKHDFYYDLPNELIAQTPIEPRDASRMMVVNRQTGEIEHKHFYD
                     VVDYLNPGDCLILNDTKVLPARLYGIKDETGAKVEFLLLMQKEKDVWEVITGPGKKAK
                     TGSKFTFNDGILYAEITAVLDNGDRIAKFTYDTDKFGNFFEVLDKIGEMPLPHYITHK
                     LENADRYQTVYAKELGSAAAPTAGLHFTPEVLQKIKDKGVKIGYVTLHVGIGTFRPVK
                     TENIEEHKMHSEHYHLPKETADLINETHNNGGRVVAVGTTCCRTLESVATYCGEICEK
                     DDWTSIFIYPGYKFKCIDALITNFHLPESTLIMLVSAFYNRDDVLKAYEEAVK"
BASE COUNT          389 a          330 c          306 g          478 t
ORIGIN      
        1 ttacagttcg tgcgcatgac tgtttggagt atgaatattc ggcaggattt tcaacacact
       61 cgtcaaaagc catggcaatg gtagagccca aatgagattg tatttgcatg ctttcttccg
      121 gtcccataaa aatacggcga ccgtctacat gagaattaaa gctgacgccc tcttctttta
      181 ttttgcggag tttagcaagc gaaaagactt gaaatccgcc gctgtctgta agaataggac
      241 ctctccaccc agtgaatttg tgaagaccgc cgaggtcgtg aattaaatca tctcccggtc
      301 ttacgtggag gtggtatgtg ttgcacagca taacctgtgt ttcgatattt tcaagatcaa
      361 cggcagaaag accgcctttt attgcacccg ccgttgctac gttcataaat gccggggtgt
      421 gtatggttcc gtgtacggtt tcaaaatcgc aaagccttgc attgccgacc tttttaataa
      481 cttttaacat aaaatctcct taaatcaaac aatgagnnnn nnnnnnttta actgcttctt
      541 cgtatgcctt caaaacgtcg tctctgttat aaaaagccga tacaagcatt ataagcgtac
      601 tttccggtag atgaaaattt gtaataagcg catcaatgca tttgaattta taacccggat
      661 atatgaaaat acttgtccaa tcatcctttt cacatatttc accgcagtat gttgccacgc
      721 tttcgagagt acggcagcag gtagttccga ccgctaccac tcttccgccg ttattgtgtg
      781 tttcgtttat gagatctgcc gtctcttttg gaagatgata gtgctcagaa tgcattttat
      841 gttcttcgat attttcggtt ttaaccggtc tgaaagtgcc tatgcctacg tgtaaggtta
      901 catagccgat ttttacgcct ttatccttta tcttttgtaa aacctccggc gtaaaatgaa
      961 gacccgccgt aggagctgcg gcagatccga gctccttagc atataccgtt tgataacggt
     1021 ctgcgttctc gagcttgtgc gtgatgtaat gcggaagcgg catttcaccg attttgtcaa
     1081 gtacctcaaa gaaattaccg aatttgtcgg tatcgtacgt aaatttagcg attctgtctc
     1141 cgttgtcaag cacagcggtt atttcggcat ataatatacc gtcgttaaat gtaaatttgg
     1201 agccggtctt tgctttcttt ccggggccgg taattacttc ccatacgtct ttttcttttt
     1261 gcattaaaag cagaaattca acctttgcgc cggtttcatc tttaataccg taaagtcgtg
     1321 ccggcaaaac cttggtgtca tttaaaataa ggcagtcgcc gggatttaaa tagtctacaa
     1381 catcataaaa atgcttgtgc tcaatttccc ctgtttgcct attgacaacc atcattcttg
     1441 acgcatcacg aggctcaata ggtgtctgcg caataagttc gtttggtaaa tcataataaa
     1501 aatcatgctt ttt
//
LOCUS       DBMDVO010000370         1510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39901_length_1510_cov_1.387509, whole genome shotgun sequence.
ACCESSION   DBMDVO010000370 DBMDVO010000000
VERSION     DBMDVO010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39901_length_1510_cov_1.387509"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="ACZPE2_05665"
     CDS             <1..425
                     /locus_tag="ACZPE2_05665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine biosynthesis
                     protein TsaB"
                     /protein_id="HIR1319370.1"
                     /translation="ISTLETIAYPFFDRNCVVCSLMDARCSQVYTALFENGKRLTPDS
                     AMLISELADSLKSIDKPIVFAGDGAAITYEKLSDSFDNIILADAQNRYQRASSVAFLT
                     EKYINSGKLPVSANELLPVYLRLPQAERELKKKKELKK"
     gene            422..862
                     /gene="rpiB"
                     /locus_tag="ACZPE2_05670"
     CDS             422..862
                     /gene="rpiB"
                     /locus_tag="ACZPE2_05670"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1319371.1"
                     /translation="MTVYLGADHGGFELKEALKKHLDAEGYKVIDCGTDSTQSVDYAP
                     IAEKTCLSVLSDEGSLGILCCGTGIGMSMAANKVKGIRAACCSDYFSAKYTRLHNNAN
                     ILCLGGRVAGAGLAAELADVFLKTEFEGGRHQRRIDQITEIENK"
     gene            931..>1510
                     /gene="upp"
                     /locus_tag="ACZPE2_05675"
     CDS             931..>1510
                     /gene="upp"
                     /locus_tag="ACZPE2_05675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1319372.1"
                     /translation="MGKVTITNHPLIQHKLTLLRDKNTGSKEFRALVSEIATLMCYEA
                     TRDLPLEEVEIETPIAIAKAKVISGKKLAFVPILRAGTGMLDGVLALVPSAKVGHIGM
                     YRDPVTVKPVPYYCKLPSDISEREVIVLDPMLATGGSAIDAIDQIKTFHPKSIKFMGI
                     IAAPEGLEALTTAHPDVDIFCAALDERLNEHSY"
BASE COUNT          437 a          308 c          344 g          421 t
ORIGIN      
        1 gcatttctac tcttgagaca atagcgtatc ccttttttga ccgaaactgt gttgtttgtt
       61 ctcttatgga cgcaagatgc tctcaggtgt atacggcact ttttgaaaac ggcaaaagat
      121 tgactcccga cagcgcgatg ctcatatcgg aattggcaga cagtttaaaa agcatagaca
      181 agcctattgt atttgcaggc gacggtgccg caataaccta tgaaaagctt tcggacagct
      241 ttgacaatat catattggcg gatgcacaaa acaggtatca gcgcgcatca agcgttgcat
      301 ttcttactga aaaatatatc aactcaggta aattaccggt tagtgccaac gagcttttac
      361 cggtttattt aagactgcct caggcagagc gagaattaaa aaagaaaaag gagcttaaga
      421 aatgacggtt tatttaggag cagatcacgg cggatttgaa ttgaaagagg cattgaaaaa
      481 acatcttgat gcagaaggct ataaagtaat agactgcgga actgattcta cacaatcggt
      541 ggattatgcg cctatagccg aaaagacttg cttaagtgtt ttatcggatg agggcagcct
      601 cggtattctg tgctgcggaa ccggtatagg tatgtctatg gcggcaaata aagtcaaggg
      661 tatacgcgca gcttgttgtt ccgactattt cagtgctaaa tatacaagac ttcacaataa
      721 tgctaatatt ctttgtctcg gcggcagggt agccggcgcc ggccttgctg cagaactggc
      781 tgatgtcttt ttgaaaaccg aatttgaggg cggccgccat caaagaagaa tagaccaaat
      841 aaccgaaatt gaaaacaaat aaaaatatct tgtcttttat tgacggctgt tatataatat
      901 tttagaaatt taaattattg gaggctaatt atgggtaagg ttactattac caaccatccg
      961 cttattcagc acaaactgac gttgctccga gataaaaata ccggttctaa agaattcaga
     1021 gcacttgttt cagagattgc aacgcttatg tgctacgagg ctactcgcga tcttcctctt
     1081 gaggaagtcg aaatcgaaac tcctattgcc attgctaaag ctaaagttat ttcgggtaaa
     1141 aaactcgcat tcgttccgat actcagagcc ggaaccggaa tgcttgacgg cgtcttggcg
     1201 cttgtcccct cggcaaaggt tggccacatc ggcatgtatc gtgatccggt tacggtaaag
     1261 cccgttccgt attattgcaa gttaccgtct gatatttcag agcgtgaagt aatcgtgctt
     1321 gatcctatgc ttgcaacagg cggttccgct attgatgcaa tagatcagat aaagactttc
     1381 catccgaaat caattaaatt tatgggtatt atcgccgctc ccgagggtct tgaagctctt
     1441 acgacggctc atcccgatgt tgatattttc tgcgctgctc ttgatgagag acttaatgag
     1501 cacagctaca
//
LOCUS       DBMDVO010000371         1509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_39956_length_1509_cov_1.110795, whole genome shotgun sequence.
ACCESSION   DBMDVO010000371 DBMDVO010000000
VERSION     DBMDVO010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39956_length_1509_cov_1.110795"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="ACZPE2_05680"
     CDS             <1..332
                     /locus_tag="ACZPE2_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIR1319373.1"
                     /translation="ARHIAEVHSDGIKLQLLHVLRGTALEKMYLENEFKTLTLDEYVD
                     ILCEILPILPPQTVIHRLTGDAPKRLLVSPLWSADKKNVLNTINRAFAERNVFEGSAY
                     ENTAAKP"
     gene            434..>966
                     /gene="rbr"
                     /locus_tag="ACZPE2_05685"
     CDS             434..>966
                     /gene="rbr"
                     /locus_tag="ACZPE2_05685"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1319374.1"
                     /translation="MAELKGSKTEKNLMAAFAGESQARNKYTYYASKAKKDGFVQIAN
                     IFEETANNEKEHAKIWFKLLHGGSIPDTAENLEDAANGENYEWTDMYAGFAKEAREEG
                     FDEIADLFLMVAAIEKEHEERYRTLLLNVKNEMVFSKDGDAIWQCSNCGHIVIGQKAP
                     DVCPVCKHPQAYFQMKA"
     assembly_gap    967..976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<977..1418)
                     /locus_tag="ACZPE2_05690"
     CDS             complement(<977..1418)
                     /locus_tag="ACZPE2_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319375.1"
                     /translation="MKKILSLLFALILLLCGCSGKDNKKGRGQKYVFPPSDAFSAEIT
                     AKQGDFTFSGLLTFSSESGLTLSYSEPSDIAGITVSLKDDTATVTCPEMEPVIVSLPG
                     SSALCFTEKVFDALKNGECDGYAFGGRIINEGTFGSTDFRVTRDK"
BASE COUNT          431 a          332 c          357 g          379 t
ORIGIN      
        1 cggcgcgcca tatagctgag gttcattccg acggtataaa attacagctt ttgcacgtgc
       61 tccggggaac cgcacttgaa aaaatgtatc tcgaaaatga attcaaaacg ctgaccttag
      121 acgaatacgt cgatattttg tgcgaaattt tgccgatttt gccgccgcaa acggtaattc
      181 accgcctcac cggagacgcg ccgaagagac ttttggtgtc gcccttgtgg tcggcggaca
      241 aaaagaatgt attaaatact ataaaccgtg cctttgcgga gcggaatgtt tttgagggca
      301 gcgcatacga aaataccgcg gctaaaccgt aggcaagttc ggattattat cgcttgccta
      361 aatactttgt cttgcttttc ggaagaaatt tcgattgcaa atatattatt tttaatagaa
      421 agaggtaaaa gctatggcag aacttaaagg ttcaaaaacc gaaaaaaatc ttatggcggc
      481 attcgcaggt gaatcacagg cgagaaataa gtacacttat tatgcgtcta aggctaaaaa
      541 ggacggcttt gttcagatag caaatatttt tgaagaaacc gcaaacaacg aaaaagagca
      601 cgccaaaatt tggtttaaac tcttgcacgg cggctcgata cctgataccg cggaaaatct
      661 cgaggacgcg gctaacggcg aaaactacga gtggaccgat atgtatgccg gttttgccaa
      721 agaggcaaga gaagagggct tcgatgagat agccgatctc tttttgatgg ttgcggctat
      781 tgaaaaggag cacgaggagc gttacagaac gcttcttcta aatgttaaaa atgaaatggt
      841 attttcaaaa gacggcgacg ctatatggca gtgctcaaac tgcggccata tcgtaatcgg
      901 acaaaaagct cccgatgttt gccccgtatg caagcatccg caggcatatt tccagatgaa
      961 agccgannnn nnnnnncttt gtctcttgtc actcgaaaat cggtgctgcc gaaggtgccc
     1021 tcgttgatta tcctgccgcc gaaagcataa ccgtcgcatt cgccgttttt cagagcgtca
     1081 aaaacctttt ccgtaaagca cagtgccgat gagcctggca aagacacgat aacaggctcc
     1141 atttcggggc aggtaaccgt tgctgtgtcg tctttaagcg acacggtgat gcccgctatg
     1201 tccgacggct cggaatagct gagcgtaaga ccgctttccg atgaaaatgt gagcaatccc
     1261 gaaaatgtaa agtcgccttg ctttgcagtt atttcggcag aaaaggcgtc tgacggcgga
     1321 aacacatatt tttgacctct gcctttttta ttatcctttc ctgaacatcc gcacaacagt
     1381 aaaatgagtg caaaaagaag cgaaaggatt tttttcatta aatatactcc ttcaatcagc
     1441 gctcaagaga cctgagtatc ttcggcagct cctctgtcat ccttgtgggc gtggtgccca
     1501 tcatggagt
//
LOCUS       DBMDVO010000372         1507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40024_length_1507_cov_1.467994, whole genome shotgun sequence.
ACCESSION   DBMDVO010000372 DBMDVO010000000
VERSION     DBMDVO010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40024_length_1507_cov_1.467994"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            148..573
                     /locus_tag="ACZPE2_05695"
     CDS             148..573
                     /locus_tag="ACZPE2_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1319376.1"
                     /translation="MSVITLHYDVPGDDFTRAGEASGDVKAHLKKLGFSPEVIRNTVI
                     ALYEGEINMVIHAGGGRIDVDIDPDVISMVLSDDGPGIPDVALAMKEGYSTAPDNVCA
                     LGFGAGMGLPNIKKYTDEMLIDTLVGKGTTMRLKVYTNR"
     gene            599..>1169
                     /locus_tag="ACZPE2_05700"
     CDS             599..>1169
                     /locus_tag="ACZPE2_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319377.1"
                     /translation="MEKSGFFHSVRLDESKCMGCTNCIKRCPTGAIRVRRGKAYIISE
                     RCIDCGECIRICPHHAKYAEKYSFDELQKYKYRIALPAPTLYGQFNNLDDTDYVLTAL
                     KKLGFDDVFEVSKGAEIVSEATRAELSHSGRKKPVISSACPAVVRLIRVRFPNLIENV
                     LDFNPPMEEAARFARERAVKRTGYSKSDIG"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1180..>1507
                     /locus_tag="ACZPE2_05705"
     CDS             <1180..>1507
                     /locus_tag="ACZPE2_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319378.1"
                     /translation="IFFITPCTAKITAIEQPICNNGSNVDGVFGIKDIYPSLLHEMEN
                     TDKTELLRDSGIIGVSWASSGGEASALLTDRYLAADGIENVIKVLEEIEDEKLSDLDF
                     IELNACP"
BASE COUNT          407 a          318 c          411 g          361 t
ORIGIN      
        1 cgtggttttt gtgcgcggta aacgccccga ccgagatatg acggagcttg ccgaaaatct
       61 tgatattata ttgcttacaa caaagctccc tatgttttcg tcatgcggcc tgctgtatga
      121 aaaaggactt ggcggaggtg aggcgaaatg agcgttatca cgcttcatta tgatgttccg
      181 ggcgatgatt tcacaagggc gggcgaggcg tccggtgacg taaaggctca ccttaagaag
      241 ctcggatttt ctcccgaggt aatcaggaat acggtaatag ctctttatga gggcgaaata
      301 aatatggtaa tacacgcggg cggcggcagg atagacgtgg atattgaccc cgatgtcatc
      361 tctatggttt tgtcggatga cggaccggga atacccgacg ttgctctggc aatgaaggag
      421 ggatattcca cggctcccga caatgtgtgt gctctcggct tcggtgccgg aatgggactt
      481 cccaatataa aaaagtatac tgatgaaatg ttaatagata ctttggtcgg caagggcaca
      541 accatgcggc tgaaggtgta tacaaacaga taaattatat aatcacggag taactgcaat
      601 ggaaaagagc ggcttttttc attccgtacg tctggacgaa agcaagtgta tgggatgcac
      661 caactgcata aagcgttgcc cgacgggagc tataagagtg cgccgcggta aggcatatat
      721 aatttcggag cggtgtatag actgcggcga atgtatcaga atttgtccgc atcacgcgaa
      781 gtatgccgag aaatactcgt ttgatgagct tcaaaaatac aaatatcgca tagccttgcc
      841 cgcgcctacg ctgtacggtc agtttaacaa tctcgacgat accgattatg tgctgaccgc
      901 gcttaaaaag cttggctttg acgatgtgtt tgaggtgtca aagggtgccg agatagtgtc
      961 agaggctaca agggcggagc tgtcacattc gggcagaaaa aagcccgtaa tatcctcagc
     1021 gtgccctgcc gtagtaaggc ttataagggt gcgtttccca aatctaatcg agaacgtgct
     1081 tgattttaac ccgcccatgg aggaggcggc gcgttttgcg agagagcgtg cggtaaaacg
     1141 gacgggatat tcaaaatccg atataggaan nnnnnnnnna atatttttca taacgccgtg
     1201 tacggcaaaa atcacagcca tagaacagcc tatatgcaac aacggctcca atgtggacgg
     1261 cgtgttcggc ataaaggaca tttatccgtc gcttttgcat gaaatggaaa atacggacaa
     1321 aacggagctt ttacgcgatt ccggtataat cggagtttcg tgggcgtcat ccggcggcga
     1381 agcctcggca cttctcaccg accgatattt agcggcggac ggtatagaaa acgttataaa
     1441 ggtgctcgag gagatagagg acgaaaagct ttcggacctt gattttatcg agcttaacgc
     1501 ctgtccg
//
LOCUS       DBMDVO010000373         1506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40052_length_1506_cov_1.501068, whole genome shotgun sequence.
ACCESSION   DBMDVO010000373 DBMDVO010000000
VERSION     DBMDVO010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40052_length_1506_cov_1.501068"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1506
                     /locus_tag="ACZPE2_05710"
     CDS             <1..>1506
                     /locus_tag="ACZPE2_05710"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1319379.1"
                     /translation="LRGVIGAGTNRMNVYTVNQATQGLSDYLNSEFKEPSVAIAYDSR
                     IKSDVFARSAASVLAANGIKVYIYRELVPTPMLSYAVRKLHCSSGIIITASHNPSKYN
                     GYKCYDPNGYQMTDAAAAKTYEFISRVDMFSGVKTADFEECVKSGKIEYISDEVYESF
                     FTEVLKARVNPGICEKAGLSVIYTPLNGTGNKPVRKILKMIGVEDVTVVPEQELPDGN
                     FPTCPYPNPEIKQAFECAINLSKTKKADLLVATDPDCDRLGIAVLDDGEYKLMTGNEV
                     GAMFTEYLLETLTEQNKLPKDPVVVKTIVTSPLISAIAAAHGATTANLLTGFKYIGEL
                     VTKLESKNEADRFVVGMEESYGYLRGSHARDKDAVVASMLVCEMASYYKLKGMSLYDF
                     MQSIYKKYGLYLNTLLNFGFEGAAGMKKMAEMMDSLRADGLKNIAGMDVITSSDYEAS
                     IETNLKTGEKAKITLPKSNVLAYTLEGGNSVIVRPSGTEPKIKIYITAVGNT"
BASE COUNT          440 a          327 c          353 g          386 t
ORIGIN      
        1 ctgcgcggag ttataggagc cggtacaaac agaatgaatg tttataccgt taatcaggca
       61 acacagggcc tctcggatta tcttaacagc gagtttaaag agccgagcgt tgccattgcc
      121 tatgattcgc gtataaaatc ggatgtattt gcacgttctg cggcttcggt gcttgccgct
      181 aacggtatta aggtatatat ctatcgtgaa cttgtaccga cgccgatgct gtcatatgcg
      241 gttcgcaagc ttcattgcag ttcgggcata atcataaccg caagtcataa tccctcaaag
      301 tacaacggct ataagtgcta cgacccaaac ggctatcaga tgaccgatgc agccgccgcc
      361 aaaacctatg agtttataag ccgtgttgat atgttctcgg gcgttaaaac cgccgacttt
      421 gaagaatgcg tcaagagcgg aaaaattgaa tatatcagcg atgaggttta tgagtcgttc
      481 tttaccgaag tgctgaaagc aagagtcaat cccggcatat gtgaaaaagc gggactctcg
      541 gtaatttata ctccgcttaa cggcaccgga aacaagcctg tccgcaaaat ccttaaaatg
      601 ataggcgtag aggatgtaac cgttgttcct gaacaggaat tgcctgacgg aaacttcccg
      661 acctgcccgt atcccaatcc cgaaatcaaa caggcctttg agtgtgcaat aaacctttca
      721 aaaacaaaaa aggcagacct tcttgttgca accgatcccg attgcgacag acttggcatt
      781 gctgtgcttg acgacggcga atataagctt atgaccggta acgaagtggg agcaatgttt
      841 acagaatatt tgcttgaaac tcttactgag caaaataaac ttccgaaaga tcctgtcgtt
      901 gttaaaacaa tagttacttc tccgcttatc tcggccattg ctgctgccca cggcgcaact
      961 acggcaaact tgcttaccgg atttaaatat ataggtgagc ttgttacaaa gcttgaaagt
     1021 aaaaatgagg ctgaccgttt tgtagtcggt atggaagaat cctacggcta tctccgcggc
     1081 tcacatgcca gagataagga tgccgttgta gcttcgatgc ttgtttgtga aatggcgtca
     1141 tactataagc ttaaaggtat gagtctttat gactttatgc agagcatata caagaagtac
     1201 ggcctttatc ttaatactct tctcaatttc ggctttgaag gtgccgcggg aatgaaaaag
     1261 atggctgaaa tgatggattc acttcgcgct gacggcctta aaaatattgc gggtatggat
     1321 gtcataacat cttcggatta tgaggcaagc atcgagacaa atcttaaaac aggcgagaag
     1381 gctaagatta ctctgccgaa atcaaatgtg cttgcataca cccttgaggg cggcaacagc
     1441 gtaatagttc gcccgtcggg aacagaaccg aagataaaga tttatattac ggcagtaggc
     1501 aacacg
//
LOCUS       DBMDVO010000374         1505 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40087_length_1505_cov_1.248575, whole genome shotgun sequence.
ACCESSION   DBMDVO010000374 DBMDVO010000000
VERSION     DBMDVO010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40087_length_1505_cov_1.248575"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(162..863)
                     /locus_tag="ACZPE2_05715"
     CDS             complement(162..863)
                     /locus_tag="ACZPE2_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012383.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1319380.1"
                     /translation="MLKFADRLLSVFFPKRCKYCGELIVPEKDVCESCESSLPRILPP
                     CCRFCGHSKEDCSCNQAKNEYSAVAAPFYYEGAAKTPIHRLKFEGKDFVARTLAQDMA
                     KTVKEQYGEKKFDIITFVPFSKSEKHDREFNQSELLAKRLGEELCLPCREMLVKLYDV
                     PRQHTLPGNKRRGNVFGIFATAEEFSYLDGKTILLADDIKTTGSTLSECAKMLKIAGA
                     KEVCAVTAAIAKRIG"
     gene            complement(883..>1505)
                     /locus_tag="ACZPE2_05720"
     CDS             complement(883..>1505)
                     /locus_tag="ACZPE2_05720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1319381.1"
                     /translation="QGYSEGAKLMLDSGDTKGASEYCEIITDETHKMNMLVLQLLELS
                     MYESGNVKLSMDKIDIRAAAEEYGEENRLKFESKGIKFINDIPSDCFGLGDSVKIRMV
                     FNNYISNACSHVSGDNIIRVYKGQSPNKGSIRICVYNSGSHIDSDDMDKIWISFYRAD
                     KSHSRAEGRFGLGLSIVGAIQKLHGEKYGAENTDDGVVFWFDIKKT"
BASE COUNT          357 a          366 c          327 g          455 t
ORIGIN      
        1 taagttgtaa tattaaagct tcggataacg caccgtcgcc cgttcaatct aacacggctg
       61 tgcctattag tgtcacgtac cgcgaaatgc tgcgtatcgg tacaagcgaa gttaatatat
      121 gtcaaaaaag tactgatacg cagcaaaagc ctgccatacc gttatcctat ccttttagct
      181 atggcggcgg ttacggcgca aacctctttt gcgcctgcta ttttcagcat tttagcacac
      241 tccgaaagcg tggaaccggt ggtctttatg tcgtctgcaa gaagtattgt tttgccgtca
      301 agatatgaaa actcttccgc tgtcgcgaat atgccgaata cattgccgcg ccgtttgttt
      361 cccgggagag tgtgctggcg cggaacatca tagagtttta cgagcatctc ccgacacggc
      421 aaacaaagct cctcaccgag ccgttttgcc aaaagctcgc tctggttaaa ttccctgtcg
      481 tgtttttcgg attttgaaaa cggcacaaag gttattatgt caaatttctt ttcgccgtat
      541 tgctctttta cggtttttgc catatcctgt gcgagagtac gtgcaacaaa atcctttccc
      601 tcaaatttca ggcggtgtat tggggttttg gcggcaccct catagtaaaa cggagcggcg
      661 acggcggaat attcgttttt tgcctgatta catgagcagt cctctttgct gtgaccgcaa
      721 aaacggcaac acggaggcaa aatacgcggc agggagcttt cgcagctttc acagacgtct
      781 ttttccggga ctatcaattc tccgcagtat ttgcaccttt ttgggaaaaa gacggaaaga
      841 agcctatccg caaatttaag caaaatattc acctccgcaa gctcaggttt tttttatgtc
      901 aaaccaaaat acaactccgt catccgtgtt ttcggcaccg tatttttcgc cgtgcagctt
      961 ttgtattgca ccgactatcg aaaggccgag tccgaaacgc ccctctgcgc gtgaatgtga
     1021 cttgtcggcg cggtaaaagc ttatccatat tttatccata tcatcgctgt ctatatgact
     1081 tccgctgttg tatacgcaaa tccgtatgct gcctttgttc gggctttgcc ctttatagac
     1141 tcttataatg ttgtcgccgg aaacgtgtga gcaggcgttc gatatgtagt tgttaaatac
     1201 cattcgtatt ttaacgctgt cgccgagtcc gaagcagtcg ctcggaatat catttataaa
     1261 tttgattccc ttgctctcaa attttaatcg gttttcttcg ccgtattctt cagcggctgc
     1321 gcgaatgtct atcttatcca tactgagctt gacgttacct gattcataca ttgaaagctc
     1381 gagaagctgt aaaacgagca tattcatctt gtgagtttcg tcggttatta tttcacaata
     1441 ttccgaagca ccttttgtgt cgccgctgtc gagcatcagc ttggcaccct ccgaatagcc
     1501 ctgta
//
LOCUS       DBMDVO010000375         1497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40367_length_1497_cov_1.502149, whole genome shotgun sequence.
ACCESSION   DBMDVO010000375 DBMDVO010000000
VERSION     DBMDVO010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40367_length_1497_cov_1.502149"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="ACZPE2_05725"
     CDS             <1..887
                     /locus_tag="ACZPE2_05725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1319382.1"
                     /translation="WYITAAVTLFGGVLAYFVSGQALKPLRSFASQTEKVQPNNLSDM
                     KISKDVLPEFKQFSHSFNNMIERLNEGFTAQRQFTGNAAHELRTPLALMQAQIELFSV
                     EHTDIKPETAEFLRLLQEQTERMSQMTNTLLEMSELRNIKCSDKIQLAPMTDEVFADL
                     APIADEKNVSLTRDGDALIIGSDTLIYRMIFNLTENAVRYTRRDSVINISIVCNENDV
                     QIRVRDNGHGIPEQYRESIFQPFFRVDKARSREHGGVGLGLALVWEIAALHGGSVRVE
                     DCSEAGTVMLVTLPKSEK"
     gene            complement(1046..>1497)
                     /locus_tag="ACZPE2_05730"
     CDS             complement(1046..>1497)
                     /locus_tag="ACZPE2_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1319383.1"
                     /translation="CAECDCKVDVSEDGQVFVSSIDLDNAKKAIFIVDTIANGPEVGA
                     VYKGIVTRLMSFGAFVEIVPGVEGMVHISQLDVKRTEKVEDVVNVGDEIIVKVLPKDE
                     QGRLNLSRREALIEVEGLTPENDLSEENQHRGGRRDFGRRPHRSDRR"
BASE COUNT          380 a          392 c          358 g          367 t
ORIGIN      
        1 attggtatat aacggcagca gtaactctgt tcggcggtgt gcttgcgtat tttgtcagcg
       61 gtcaggcact gaagccgctt cgttctttcg cttcgcaaac ggaaaaagtt cagccgaata
      121 atctgtccga tatgaaaatc agcaaggacg tcttgcccga attcaaacaa ttcagccatt
      181 cttttaacaa tatgattgaa cggcttaacg aggggttcac ggcgcagcgg caattcacgg
      241 ggaatgccgc acatgagctg cgcacacccc ttgccttaat gcaggcacaa attgagcttt
      301 tctccgttga acacacagat ataaaacccg aaacggcgga atttttgaga cttttacaag
      361 agcaaacgga acgtatgtcg cagatgacga ataccctgct tgagatgagc gagcttcgca
      421 acattaaatg cagcgacaaa atacagttag ctcccatgac agatgaagtt tttgccgatc
      481 tcgccccgat tgccgatgaa aagaatgtat ctcttacacg cgacggcgat gctctcataa
      541 tcggcagcga tacattgatt taccgtatga tatttaacct caccgaaaac gccgtccgtt
      601 acacccgacg cgattcggta attaacattt cgatagtctg caacgaaaac gacgttcaaa
      661 tacgcgtaag ggacaacggt cacggaatcc ccgaacagta cagagagagt attttccagc
      721 cgtttttccg tgttgacaag gcgcgcagca gagagcacgg cggagtgggt ctcgggcttg
      781 cgctcgtatg ggaaattgcc gcgcttcacg gcggttccgt ccgagtcgaa gattgctcgg
      841 aagccggcac ggtaatgctc gttacacttc cgaaaagcga aaaataaacg tacagtgttt
      901 tctcgccgaa aacccgcaaa aaatccctcc cgatggcaaa acatcgagag ggatttttaa
      961 tccgaatata cggtatttta ttttgcattt cccttcgctg tgcgccgaat aattcacgct
     1021 cgcacggcat atcggggctc ttttttcatc ggcgatccga gcggtgcggt cttctgccga
     1081 agtcgcgtct gccgcctctg tgctgatttt cctcggagag gtcgttttcg ggggtgagac
     1141 cctcaacctc tataagtgcc tcgcggcgag agagattgag tctgccctgc tcgtccttcg
     1201 ggagaacctt gacgattatc tcatcgccga catttacaac gtcctcaacc ttttcggtgc
     1261 gcttaacgtc gagctggctg atgtgaacca taccctctac tccgggtact atctcaacaa
     1321 acgcgccgaa gctcataagg cgtgtaacga ttcccttgta aacagcacct acctcggggc
     1381 cgttagctat tgtatcgaca atgaagattg ccttctttgc attgtcaagg tcaatcgagg
     1441 aaacaaatac ctgaccgtcc tcgctcacgt caaccttgca gtcgcactcg gcgcaaa
//
LOCUS       DBMDVO010000376         1497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40382_length_1497_cov_1.130372, whole genome shotgun sequence.
ACCESSION   DBMDVO010000376 DBMDVO010000000
VERSION     DBMDVO010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40382_length_1497_cov_1.130372"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(201..712)
                     /locus_tag="ACZPE2_05735"
                     /pseudo
     CDS             complement(201..712)
                     /locus_tag="ACZPE2_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513678.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<723..1366)
                     /locus_tag="ACZPE2_05740"
     CDS             complement(<723..1366)
                     /locus_tag="ACZPE2_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1319384.1"
                     /translation="MKMSLKKVLAAVLALTFLAACFTACGSKGTGSSSSDGKTLKIGS
                     SGPLTGDAAAYGIAVKNGIQLAVDEINAAGGVNGMKLEFQMEDDEADAEKAVNAYNTL
                     KDNGMKVFIGTVTTGACLSVVSQTKADNMFQITPSASAAKVIANDNCFQVCFTDPNQG
                     TASADYIASKGLATKVAVIYNSSDAYSSGIYDNFKAEAAAKGLDLVASEAFTGD"
BASE COUNT          425 a          343 c          313 g          406 t
ORIGIN      
        1 aaactaaaaa tgtttcttgt ttaaaataca aatagtataa catatcctct ttgccattac
       61 aacgagaaaa atatgcaaaa atgaataatc aggcggcgcg cttttcatta tatttcaccc
      121 aattaaagcg ataatgcagg aaacaaaaag ggccggcagc cgtaagctgc cgacccgtat
      181 tcagccttta accgaggaaa tcagtccatc ggctgatatt cgccgttttc aatcttaacc
      241 gctttcggct ctttgtttac ttcgcctgtt gctttccagg tcatacctga acctgttaca
      301 ccgtcaacgg tgatttcggt catggctccc ttgagagcat tgcaaatatc ggaaacgctc
      361 atatcgcctg taacacctgc tttttccgca gctgccttta caacataaac agcgtcataa
      421 gcgtctgccg caaactgatt aagagtagac gggtcatact ttgcattgta agcggcaacg
      481 aacttcttgg ttgtctcgtc ggtagcatta gctgcaaacg gggtaagaag cattacgttt
      541 tccgcaagag acttatcaaa gttctcgatg ttaagaagtc cgtcaagacc gtcgcagccg
      601 aagaacttag cggtaatacc ggcgttctta gcctgagaaa ggataagaga agcttcctga
      661 taataaatcg ggaggaatat aagctctgcg ccggaagcct taatctttga aannnnnnnn
      721 nnctgtcgcc tgtgaatgct tcggaagcta caagatcaag accctttgca gccgcttcag
      781 ccttaaagtt gtcataaatg cccgaagaat aagcgtcgga gctgttatag ataacagcta
      841 cctttgtagc aagacccttg cttgcgatat agtctgcgga agcagtaccc tggttggggt
      901 ctgtaaagca aacctggaag cagttgtcat ttgctattac cttagctgcc gaagcagaag
      961 gtgttatctg gaacatatta tcagccttag tctggctgac aacagaaagg caagcacctg
     1021 tggtaacggt acctatgaat accttcatac cgttatcctt aagagtattg tatgcgttta
     1081 ctgctttttc ggcatcggct tcatcgtcct ccatctggaa ctcaagcttc ataccgttta
     1141 cgccgcctgc agcgttaatt tcgtcaacag caagctgtat accgttttta acggcaatac
     1201 cgtaagctgc cgcgtcgcct gtaagcggtc cgctggaacc tattttaagt gttttaccgt
     1261 ctgaagagct gctgccggtt cccttactgc cgcaagccgt aaagcaagct gcaaggaaag
     1321 tcaaagcaag aactgctgcc agaacctttt tcaatgacat tttcataaaa tgacctcctg
     1381 tagctaattt tgcaagattt aaaagcgaat cctgcaataa atcatttatg atgaaaatta
     1441 tcttacacgc aaaaatatga tttgtcaata aatagccgaa atttagtcgc gaaaaga
//
LOCUS       DBMDVO010000377         1491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40601_length_1491_cov_1.228777, whole genome shotgun sequence.
ACCESSION   DBMDVO010000377 DBMDVO010000000
VERSION     DBMDVO010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40601_length_1491_cov_1.228777"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1491)
                     /locus_tag="ACZPE2_05745"
     CDS             complement(<1..>1491)
                     /locus_tag="ACZPE2_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR1319385.1"
                     /translation="AICINFGTAQKYGGVCNLRLDDTNPQKEDTEYVDAIKEDIEWLG
                     FKWNKCLYASSYFDFIYECAVKLIKDGKAYVDDLSADEIREYRGTLTEPGKNSPYRDR
                     TTEENLDLFKRMTNGEFPNGARVLRAKIDMASPNINMRDPVIYRIAHVPHHQTGDKWC
                     VYPMYDFAHPLSDYKECVTHSLCSLEFENHRPLYDWFLRELGFKNVPRQIEFARLNLN
                     YCLTSKRKCLALVNEHIVDGWDDPRMATISGMRRRGYPAKAIRTFCEKIGVSKAYSVV
                     SMDLLESCVRDELNENAPRAMAVLDPLKVVIDNYPEGKTEEIEVEVHPNHPEMGTRKV
                     LFGRELFVEREDFMAEPVKKYFRLFPGNEVRFKSAYFVKCVGFDCDENGEVTTVHCTY
                     DPESRGGNSPDGRKVKGTIHWVSASGSFKATVNMYDRLFNVPNPSDESTGSFKDNLNP
                     DSLKVLTGCLIESGLKDVKPGDVFQFMRKGYFCVDKNSTCDNIIFNL"
BASE COUNT          352 a          362 c          339 g          438 t
ORIGIN      
        1 caggttgaat ataatattat cacaagttga atttttgtca acacagaagt agcccttgcg
       61 cataaactgg aaaacatcac ccggttttac gtcctttaag ccgctctcta taaggcatcc
      121 cgttaacacc tttaaagagt cgggatttaa gttgtcctta aagctgccgg tgctttcgtc
      181 tgacgggttc ggcacgttaa agagcctgtc gtacatattt acggtagcct taaagctgcc
      241 gcttgccgat acccaatgaa tggttccctt tacctttctg ccgtcgggtg aatttccgcc
      301 gcggctctcg gggtcgtaag tgcagtgaac cgtggtgact tcgccgtttt cgtcacagtc
      361 aaagccgacg catttcacaa agtatgcact cttgaatctt acctcgttgc cggggaaaag
      421 cctgaaatac tttttaacag gctcagccat aaagtcctcg cgctcaacaa aaagctctct
      481 tccgaaaaga acttttcttg tgcccatttc ggggtgatta gggtgaacct caacttctat
      541 ttcttcggtc ttaccctcgg gataattgtc tataactacc ttgagcgggt cgagaacagc
      601 cattgctctc ggagcgtttt cgttaagctc gtcacgtacg caggattcga gaagatccat
      661 acttactacc gaataagcct ttgaaacacc gattttttcg cagaaggtgc gtattgcctt
      721 tgccgggtat ccgcggcgtc tcataccgct tattgtagcc attctcgggt catcccagcc
      781 gtcaactatg tgctcgttta caagcgcgag acatttgcgt ttgcttgtga gacagtaatt
      841 caaattgagt cttgcaaatt ctatctgtct cggaacattt ttaaagccga gctcacgaag
      901 aaaccaatca taaagcggtc tgtgattttc aaattcgaga gaacacagcg agtgtgttac
      961 acactctttg tagtcggaga gcgggtgtgc gaaatcatac atgggatata cgcaccactt
     1021 gtcgccggtc tgatgatgcg gaacgtgcgc aatgcggtaa ataacggggt cgcgcatatt
     1081 tatgttaggc gacgccatat cgatttttgc gcgcaaaact cttgcaccgt tcgggaattc
     1141 gccgtttgtc atacgcttga aaaggtcgag gttttcctct gttgttctgt cgcggtaagg
     1201 actgttttta ccgggctcgg taagagttcc gcggtattct cttatttcgt ctgcggagag
     1261 gtcgtcaaca tatgccttac cgtccttaat aagctttacc gcacattcat agataaaatc
     1321 aaaatagctc gacgcataaa ggcacttgtt ccatttgaag ccgagccact ctatatcctc
     1381 ttttatggcg tctacgtatt cggtgtcctc tttctgcgga tttgtgtcat cgagacgcaa
     1441 attgcatact ccgccgtatt tctgcgccgt accgaagttt atgcaaattg c
//
LOCUS       DBMDVO010000378         1489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40657_length_1489_cov_1.280259, whole genome shotgun sequence.
ACCESSION   DBMDVO010000378 DBMDVO010000000
VERSION     DBMDVO010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40657_length_1489_cov_1.280259"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="ACZPE2_05750"
     CDS             complement(<1..593)
                     /locus_tag="ACZPE2_05750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1319386.1"
                     /translation="MQKTSSRNVILLVFSLVFYAWGEPKYIFLMIATAFVDYVFGLLI
                     ERFRGRWQSKLFLTLAAVITLSSLALFKYSSFFSENVNSLFSLNLPSLKLALPIGISF
                     YTFQALTYVIDVYRGSVKVQPSFYKLLLYVSMFPQLIAGPIVRYSDVEEQIEERTVTL
                     KNISAGVMRFSAGLLKKAIIANFAGQLTEKLIGGDLTG"
     gene            complement(778..1473)
                     /gene="sigE"
                     /locus_tag="ACZPE2_05755"
     CDS             complement(778..1473)
                     /gene="sigE"
                     /locus_tag="ACZPE2_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR1319387.1"
                     /translation="MLAKIKKLFLLNDVHYINGPETLPPPLSKKEEEKLLSDILIGNG
                     NDVEKARQALITHNLRLVVYIAKKFENSGVPVEDLISIGTIGLIKSVNTFRPEKNIKL
                     ATYSSRCIENEILMHLRKTNSHKGDVSIDEPLNVDWDGNELLLSDVLGSEPDEVNRNI
                     EYDDEKKLLLKTVDALPDREKLIMNMRFGLGRYNEHTQKEVADILGISQSYISRLEKR
                     IIARLKTEIEKVS"
BASE COUNT          440 a          331 c          297 g          421 t
ORIGIN      
        1 ccggtcaaat ctccgcctat gagcttttcc gtgagctgac ccgcaaaatt tgctataatt
       61 gccttcttca aaagtccggc cgaaaaacgc ataacaccgg cggaaatatt tttcagcgtg
      121 actgtacgct cttctatttg ctcctccaca tcgctgtagc gaactatggg tcctgctatc
      181 agctgcggga acatagacac atacaagagt aatttgtaaa acgacggctg taccttgacg
      241 ctgccgcggt atacgtcgat aacataggta agtgcctgaa aggtataaaa tgaaattcct
      301 atcggcagcg caagcttgag gctcggcaaa ttcagcgaaa aaagagaatt aacattttcg
      361 ctgaaaaacg aagaatattt aaaaagcgcg agcgaagaca aagttataac cgccgcaaga
      421 gtgagaaaaa gctttgactg ccatctgccg cgaaagcgtt cgataagaag cccgaaaacg
      481 taatccacaa acgcggtggc aatcatcaga aatatatatt taggctcgcc ccatgcataa
      541 aacacaagag aaaagacgag taaaatcacg ttgcgcgagg atgttttttg cactatgtaa
      601 tagcaaataa agcacagcgg taaaaacagg tataaaaaag tcagacttga aaagaccaaa
      661 agatatcacc acaaacatat attagcttta gtattgtatc acaaaattct caaaattaac
      721 agcctttttt gataaatatt atcaacgcac cgtatttcag ctccaaaggc gtatttttca
      781 cgaaactttt tctatttccg tcttcaatct cgctattatt cgtttttcca atcttgaaat
      841 atagctttgg gaaatgccca agatatccgc cacttccttt tgagtgtgtt cattatatct
      901 gccgagaccg aagcgcatat tcataatcag cttttcacgg tccggcaagg cgtcaaccgt
      961 ttttaaaagg agcttcttct cgtcatcgta ttcgatattt cgatttactt cgtccggctc
     1021 gctgccgagc acatccgaca aaagcaactc attcccgtcc caatcgacat tcaacggctc
     1081 atctatggaa acatcgccct tatgcgaatt tgttttgcga agatgcataa gtatttcgtt
     1141 ttcgatgcag cgcgaggagt aagtagcaag cttgatgttt ttttcgggtc tgaaggtatt
     1201 tacggattta ataagaccta ttgtgccaat cgaaatcaag tcctctaccg gcacaccgga
     1261 attttcaaat ttttttgcaa tataaaccac aagtctcagg ttgtgagtta taagggcttg
     1321 cctggccttt tcgacatcgt ttccgttgcc gatgagtata tcggacagta gcttttcctc
     1381 ctcctttttt gaaagcggcg gaggcagcgt ctcaggtccg tttatgtagt gtacgtcatt
     1441 taaaagaaaa agctttttaa tctttgccag cagttttttt agcgcgttc
//
LOCUS       DBMDVO010000379         1486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40766_length_1486_cov_1.409386, whole genome shotgun sequence.
ACCESSION   DBMDVO010000379 DBMDVO010000000
VERSION     DBMDVO010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40766_length_1486_cov_1.409386"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="ACZPE2_05760"
     CDS             <1..426
                     /locus_tag="ACZPE2_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319388.1"
                     /translation="SEKSLSGVRRVKETYRISSAVTATDRIKDGFSFDSVVSLNGNSA
                     EITDGKLKITVQDSRVYVSFGDSLSEISFGDVNDGSDAGLLICRGLTGYEKSDIILVS
                     TDKTDIGDLPSQKVILTSQNGTVLFTLSHNGKMTYRRMA"
     gene            426..1466
                     /gene="holA"
                     /locus_tag="ACZPE2_05765"
     CDS             426..1466
                     /gene="holA"
                     /locus_tag="ACZPE2_05765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1319389.1"
                     /translation="MPSLDTKQLKAQLKEEKFERSYLIYGEEDYLKHYYSELIASKCV
                     TSGMEGFNLKRFDYSQGSTFEEVRAASETLPAFGGYACVVAKDYPLDSIYSSDKSAFE
                     SFLKELPDSTVVIFLQDTVSVDAKRSAKYKSIIAQFQKYGAEICFDRLDVTSLTKIIM
                     SGAAKRGCNIDRETAAYLIETSGNDLTILQNETDKLCNFKNGDQITKTDIDEVCVKSL
                     EASVFDLSRAVTKNDGKKAFSVLHKLFAEKEKPELILGTMIMAFVDMYRAKVAVSSGE
                     KANFAASCYNYRGREFRLRNASYDSRNISIKTLRKNLDSLNEADRKLKIRVTDEKIIL
                     EKLLVELLRSEQ"
BASE COUNT          461 a          305 c          362 g          358 t
ORIGIN      
        1 agcgagaaaa gcctttccgg tgtgagacgc gtaaaggaga cataccgtat atcgtcggcg
       61 gttacggcaa ccgaccgcat aaaagacggc ttttcctttg attccgttgt ttctttgaac
      121 ggaaattctg cggaaattac cgacggtaag cttaaaatca ccgtacagga cagccgagtt
      181 tatgtttcct tcggtgactc tctctcggag atatcattcg gcgacgtcaa tgacggctcg
      241 gatgcagggc ttttaatttg ccgcgggctt acgggatacg aaaaatccga tataatactt
      301 gtttcaaccg ataaaaccga tataggggat ttaccgtcac aaaaggttat attgacttca
      361 caaaacggga cggtgctttt taccttgtcc cataacggaa aaatgacata ccggaggatg
      421 gcataatgcc gagtcttgac acaaaacagc tcaaagcgca gctgaaagaa gaaaaatttg
      481 agcgttcata tttaatatac ggagaagagg attatttaaa gcattattat tcggagctta
      541 tagcctcaaa atgcgttacg tcgggtatgg agggctttaa cctaaagcga ttcgattatt
      601 cgcagggcag tacctttgaa gaagtgcgcg ccgcatcgga aactcttccg gctttcggcg
      661 gatacgcctg cgttgtagct aaggattacc ccttggattc aatatattca tcggataaaa
      721 gcgcatttga atcgtttctc aaggagcttc ccgacagtac cgtggtaata tttttacaag
      781 acacggtgag cgttgatgca aagcgtagcg caaaatataa aagtatcatt gcgcagtttc
      841 aaaaatacgg agcggagata tgctttgaca ggctcgatgt tacgtcactt acaaaaatta
      901 taatgtcggg tgccgcaaag cgcggctgca atatagaccg cgagacggcg gcatatctta
      961 tagagacaag cggaaacgac cttacgattc tgcaaaacga gaccgataag ctgtgcaatt
     1021 tcaaaaacgg ggatcagata acaaaaacgg acattgacga ggtgtgcgta aaatcacttg
     1081 aagcaagcgt gtttgacctg tcaagggctg taacgaaaaa cgacggcaaa aaggcgtttt
     1141 cggttcttca taagctgttt gcggaaaagg aaaagccgga gctgatttta ggcactatga
     1201 taatggcgtt cgtcgatatg taccgcgcca aggttgccgt gtcatcggga gaaaaagcga
     1261 attttgccgc aagctgttac aattaccgcg gcagggaatt caggcttcgc aacgcctctt
     1321 acgacagccg caatataagc ataaagactc tgcgtaaaaa tctcgattcg ttaaatgaag
     1381 cggacagaaa actcaaaatc agagtaaccg acgaaaaaat aattctcgaa aagctgcttg
     1441 tagagctgct tcggagtgaa cagtaaatga tacaaataaa gcaagc
//
LOCUS       DBMDVO010000380         1486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40776_length_1486_cov_1.108303, whole genome shotgun sequence.
ACCESSION   DBMDVO010000380 DBMDVO010000000
VERSION     DBMDVO010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40776_length_1486_cov_1.108303"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>540)
                     /locus_tag="ACZPE2_05770"
     CDS             complement(<1..>540)
                     /locus_tag="ACZPE2_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HIR1319390.1"
                     /translation="FRKKLTGAILRNIRQSGLDRILFFDFDAVNEIGDKVNLSVCVEI
                     MAQHSNIILIGNDRKIIDAVKRVDENKSSVREILPSVTYVLPPQQKKCNILKDEVKDI
                     CTSVLLNSHLKMSKSLLFSIEGSSPLMCREIAYEAFDEDLAVSECDSDILKKAVSELK
                     AYLVSDKVGVTVLFDVDGKP"
     assembly_gap    541..550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<551..738)
                     /locus_tag="ACZPE2_05775"
     CDS             complement(<551..738)
                     /locus_tag="ACZPE2_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HIR1319391.1"
                     /translation="MALDGITLHFIKDEIAEKLIGARVERVNQPSRLELVFNMRTRNG
                     AYKLLMSAEPSSARVHLTS"
     gene            complement(751..>1486)
                     /locus_tag="ACZPE2_05780"
     CDS             complement(751..>1486)
                     /locus_tag="ACZPE2_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1319392.1"
                     /translation="SEIYDYIDSFAPFSLQLEWDNSGILVNCCDCVISKALVTLDITA
                     EVLNEAIDGKYHLIVSHHPVIFGSLSALTEKTPAAICFKNGISVISAHTNYDLSPIGV
                     NKVLAQKLNLHNVVSSNDSCFMIGELDKPMTSDMFSEYVSKCLSAHLRYNDCTKEIKT
                     VAVCGGAGSDFASDAKKAGADALVTGDGKYHEFLDATDEEILLVSAGHYETEFPAVVS
                     LCKLLDDKFKDIQFKVAEQKTPIKNI"
BASE COUNT          448 a          330 c          250 g          448 t
ORIGIN      
        1 ggtttaccgt caacatcaaa taatacggta acaccgactt tatcgctcac caaatatgcc
       61 tttaattcgg atacagcttt tttgagtata tcgctgtcgc actccgaaac cgcaagatct
      121 tcatcaaaag cttcgtacgc aatttcacga cacataagag gcgaagaacc ctcaattgaa
      181 aacaacaaag acttgctcat tttcaaatga gaattcaaca gtaccgaagt acaaatatct
      241 ttgacttcgt ccttgagaat attgcatttt ttctgctgcg gcggcaacac gtatgttacg
      301 gaaggcaata tttctctgac ggatgattta ttttcgtcaa ccctttttac cgcatcgatt
      361 atttttctgt cattgcctat aagtataata ttactgtgtt gagccattat ttcgacacaa
      421 actgagagat taaccttatc tcctatttcg ttaactgcgt caaaatcgaa aaacagtatt
      481 ctgtcaaggc ccgattgacg aatgtttcgg agaattgctc ctgtaagttt ttttctaaaa
      541 nnnnnnnnnn gaggtcaaat gcaccctcgc cgagctcggt tctgccgaca taagcagttt
      601 atatgcaccg tttcttgttc gcatattaaa gaccaattca agccttgacg gctgattgac
      661 ccgttcaact ctcgctccta ttaacttttc ggctatttcg tctttaataa aatgtaatgt
      721 aataccgtca agagccataa tgatatatcc tcaaatattc ttaatcggag ttttttgttc
      781 cgcaacttta aattgtatat ctttaaactt atcgtcaagc aatttacaca agctcactac
      841 agccggaaac tcagtctcat aatgcccggc agaaacaagc aaaatttctt catcggttgc
      901 atccagaaac tcatgatatt ttccgtctcc cgtaacaagt gcatcagcgc cggctttttt
      961 tgcgtccgat gcgaaatccg agcctgcacc gccgcaaacc gcaacagtct ttatttcttt
     1021 tgtacagtca ttatatctaa ggtgcgccga aagacatttg ctcacatatt ccgagaacat
     1081 atccgaggtc atcggtttat caagctcacc tatcataaag cagctgtcat tacttgaaac
     1141 aacattatgc aaattaagtt tttgtgccag aaccttattt acaccaatgg gagataaatc
     1201 atagttagtg tgggcggaaa taacagatat accgttttta aagcaaattg ctgccggtgt
     1261 tttttcggta agtgcagaca aagaaccgaa aattaccgga tggtgggaaa caataaggtg
     1321 atactttccg tcaatcgctt cattcaaaac ttctgcagta atatcaagcg taaccaaagc
     1381 ctttgaaata acacagtcac agcaatttac caaaataccg ctgttatccc attcaagttg
     1441 taaagaaaac ggtgcaaaac tgtcaatata atcatatatt tcgctt
//
LOCUS       DBMDVO010000381         1483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40881_length_1483_cov_1.008683, whole genome shotgun sequence.
ACCESSION   DBMDVO010000381 DBMDVO010000000
VERSION     DBMDVO010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40881_length_1483_cov_1.008683"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(60..1073)
                     /locus_tag="ACZPE2_05785"
     CDS             complement(60..1073)
                     /locus_tag="ACZPE2_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR1319393.1"
                     /translation="MKYSTADDLPIILKEFLNHIYVVKNKSSLTIDEYALDLKMFLRF
                     LKRQKALVPSSEEFDKIDITDVDIDFLRNVTLMDAYAFLSYCKNERNNDSAARARKVT
                     SIRMFYKYLTVQRMFFKENPMLELETPKLKKSQPKYLTLDESLKLLGSVDGKHKERDY
                     CILVLFLNCGLRLSELVSINYNDIRDNNTITITGKGNKQRTIYLNDACMDAIKNYMAV
                     RPKDGLKEKDKDALFLSSRLQRISPKTVQHIVYEYLDKAGLGNRGFSAHKLRHTAATL
                     MYQHGHVDVLVLKDILGHENLGTTEIYTHIVDEQLRKASEANPLAEVKASKANKNPSK
                     KEE"
BASE COUNT          486 a          272 c          235 g          490 t
ORIGIN      
        1 atccgaaata ctgatttcaa taatttcgac aataaatata agatttttag ccgaaaagct
       61 cactcttcct ttttagaagg atttttattc gctttacttg ctttaacctc ggccaacgga
      121 ttagcttcgg aggcttttct tagttgttca tcaacaatat gagtataaat ctccgttgtg
      181 ccgagatttt catgacccaa tatgtccttt aataccaata catcaacatg cccgtgctgg
      241 tacataagag ttgcggcggt atggcgaagc ttatgtgccg aaaaacctct gttgccgaga
      301 cccgctttgt ccaaatactc ataaacaata tgctgtacgg ttttaggact tatgcgttga
      361 agccgtgagc ttaaaaacaa cgcgtctttg tctttttctt ttaaaccgtc tttgggccga
      421 accgccatat agtttttaat agcatccata caagcgtcat ttaaatatat tgtacgttgt
      481 ttgttgcctt tacctgttat cgtaattgtg ttattgtctc ttatatcatt atagttgatt
      541 gaaacaagtt ctgacaaacg cagtccgcaa tttaaaaaca aaacaagtat gcaataatcc
      601 cgctctttat gttttccgtc aacgctgccg agcaatttaa ggctctcatc aagagtcaga
      661 tatttcggct gtgatttttt gagcttaggc gtttcgagct caagcattgg gttttcctta
      721 aaaaacattc tctgtacagt cagatattta tagaacattc tgattgaagt aacttttcgt
      781 gctcttgccg ccgaatcatt attgcgctca tttttgcaat atgataaaaa ggcatatgca
      841 tccattaaag ttacatttct caaaaagtct atatcaacat ctgttatgtc aatcttgtca
      901 aactcctcag aggaggggac aagcgctttt tgcctcttta aaaagcgcaa aaacattttt
      961 aaatccaacg catattcatc aatggtaagc gatgatttat ttttaacaac ataaatgtgg
     1021 tttaaaaatt ctttaagtat aataggcaga tcgtcagcag tactgtattt cattattaaa
     1081 gagcacctcc gtgtgaaacc gtttcacaaa tgctgattga atatatgaat ttgccataac
     1141 accgaaccgg agttcgtaaa ttatgcccag gcaaataaag taaggataat acgttattaa
     1201 attatcactt tatcaattat acaataaact gcaagaattg tctatttaca gaataattta
     1261 gcttctgcta attctgcaaa tccaaacaat aataatcatt aagacaatgc aatgaattaa
     1321 catagagaaa actaattaag gaaataatta ttaaaataat ttataatatt ttttataaag
     1381 caaataatgc cgtaatttaa gtaaaatgta gataacattg catataaagt taacaacgcc
     1441 gacttaagcc atatattaac tttaaaaggc aaaaagtccg tca
//
LOCUS       DBMDVO010000382         1483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40883_length_1483_cov_0.945007, whole genome shotgun sequence.
ACCESSION   DBMDVO010000382 DBMDVO010000000
VERSION     DBMDVO010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40883_length_1483_cov_0.945007"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            370..>1483
                     /locus_tag="ACZPE2_05790"
     CDS             370..>1483
                     /locus_tag="ACZPE2_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013739.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1319394.1"
                     /translation="MENKNQRICIIGGGPAGTSMAMYLEKNGYDNYVIYEKSNRVGGK
                     AFSPKMKVKNAQGKWEDRTIEMGAVMGCDTYFAVHECEEFGGTTHVGGPPMGRKFMKV
                     DGTPQKMSPIAMLKKLSKMKKLSKLLETKYKGYDVNGHRGISEGRYEGPCPSPEMKLA
                     HIEGVNPNLKDLSMPFSEFLKLNHCEDATTVWKGPFTSFGYGYFDEIPAAYVLKYLDA
                     FTVKQFLSTGKLWTWYDGTQSIWEGVNNHLKHPALLNSEVTNIKRENDKVFVTVNGKT
                     EEFDKLIICTPLELFLGYGDPREEEKDLFSKIVHKEYFTMAVRPEEGNAPEISYYFFE
                     NMTNETRGHLMVFYHRWSDGVTDQPLVTFTLRNHEGM"
BASE COUNT          470 a          308 c          307 g          398 t
ORIGIN      
        1 cgggacatat ttgccggaaa acgttatttt ttgcggtttc ggaattatat tcataaaatt
       61 cacccgtttc tcattgagtt ttgttttcaa aataagaata tctataatat aacacacaaa
      121 ttataaaatt caaatatttt cgttttacga aacgacatat tttataaaca catcttaatt
      181 tattgtatat tataccgact gaacaatcat tttaagacaa aattatgtaa attttaacgt
      241 caaaaaggtc gaaaattcta ttgacgttga aataaagcaa gactataatt aaattgttaa
      301 agatttaacc gatacggtgt tgtggataaa aaatccgtac cgaattttta aaagagggag
      361 attcttttta tggaaaacaa gaatcagcgc atctgtataa tcggcggagg ccctgccggc
      421 acaagcatgg caatgtacct tgaaaaaaac gggtatgata actatgtaat ctatgaaaaa
      481 tccaatcgcg tcggaggtaa ggcattttcc ccgaaaatga aggttaaaaa tgcccagggc
      541 aaatgggagg accgcaccat agagatgggc gcggtaatgg gctgcgacac ttactttgca
      601 gttcatgagt gtgaggaatt cggcggtaca acacacgtcg gaggcccacc tatgggacgc
      661 aaatttatga aagttgacgg cactcctcag aaaatgtcgc ccatagctat gctcaaaaag
      721 ctatccaaga tgaaaaaact gtcaaagctt cttgaaacaa aatacaaggg ctatgatgta
      781 aacggtcacc gcggaatttc ggagggacgc tatgaaggtc cctgcccctc tcccgaaatg
      841 aaactcgctc atattgaggg cgttaacccc aaccttaaag acctttcaat gccgttctcc
      901 gaatttttaa agctcaacca ttgcgaggac gccaccacgg tttggaaggg tccgttcact
      961 tctttcggct atggttattt tgatgaaatc cccgccgctt atgtcttaaa atatcttgac
     1021 gcatttactg ttaagcaatt cctttcgaca ggtaaactgt ggacttggta cgacggcaca
     1081 cagagcattt gggagggcgt taacaatcac cttaagcatc cggcgctctt aaattcggaa
     1141 gttacaaaca tcaagagaga aaacgataag gtatttgtaa cggtaaacgg taagaccgaa
     1201 gaatttgaca agctcataat ctgcactccg cttgagctgt tcctcggcta cggcgatccg
     1261 cgtgaagaag aaaaggacct cttctcaaaa atcgtacata aggaatactt tacaatggcc
     1321 gttcgccccg aagaaggcaa cgctcccgaa atttcatatt acttctttga aaatatgact
     1381 aacgagaccc gcggtcatct tatggtattc tatcaccgtt ggtccgacgg cgtaacagat
     1441 cagccgcttg ttacctttac tcttcgtaac cacgagggta tgg
//
LOCUS       DBMDVO010000383         1481 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_40952_length_1481_cov_1.090580, whole genome shotgun sequence.
ACCESSION   DBMDVO010000383 DBMDVO010000000
VERSION     DBMDVO010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40952_length_1481_cov_1.090580"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            31..>1481
                     /locus_tag="ACZPE2_05795"
     CDS             31..>1481
                     /locus_tag="ACZPE2_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024117.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1319395.1"
                     /translation="MSTTCSVSDLLRSIYERTAYPSIVMSMPDGDTRRLNLLLLLQYA
                     EKYESNSDLGLSGFVRYLTRTRDSKSSIDSSTGVSEYADVVRIMTIHASKGLEFPVVI
                     IAGCGKKMNTSSLTDSLIIDPAAILGAGMKRIVAETGRVFETLQHRACRLSIRDSERA
                     EELRVLYVAMTRARERLIMVGSSENPENLVKKCGASLCGDKTPSPVLIKKAGSYLEIL
                     ILSLMWHKDAKLLREFAAVSERFTYNPNFSLNVEYVTSVSTGEYITEDEDAVAPDENL
                     LRDIKRRTSYVYGYSRLSGYAAKMSASSVENSSRSLDWYFTAKPDFLCKNDMTPAMRG
                     TAAHHFLEVCDLDMGAQSVEKETKRLVSLGKLTEVEAAAADKKMLERFFESNLFKRIK
                     NADKIYREQKFTIAVPLGELNSDVPKEFSSEKAVVQGVIDCAFFENGETIVLDYKTDN
                     VKDEEELKRRYSGQLSIYKRAAEEIFSQPVKETL"
BASE COUNT          421 a          314 c          368 g          378 t
ORIGIN      
        1 tttataaaga gtctcactga atacagagag atgagtacca cctgctcagt cagcgacctc
       61 ttgcgctcta tatatgagag gacggcatat ccgtcaatag ttatgtcaat gcctgacggc
      121 gatacgagac gtttgaattt attgctgctg ctacaatatg ccgaaaagta tgagagtaat
      181 tccgatttgg ggctttcggg ctttgttcgt tatttaaccc gtacaagaga cagcaagagc
      241 agcattgact cttccacggg cgtttccgaa tatgccgatg ttgtaagaat tatgactatc
      301 catgcgagca agggtcttga atttcccgtt gtcattatcg caggctgcgg aaagaaaatg
      361 aataccagtt ctctgacgga ttcgctgatc atcgaccctg ccgcaattct cggagcgggt
      421 atgaaacgaa ttgttgccga aacaggcaga gtgtttgaaa ctcttcaaca tcgcgcctgt
      481 cgtctttcga tacgagacag tgagcgggca gaggagctgc gagttttgta tgttgcaatg
      541 acaagagcga gggaacggct cataatggtc ggctcctccg aaaacccgga aaacctcgta
      601 aaaaaatgcg gggcatccct ttgcggtgac aaaacgccgt cgcccgtact cataaaaaaa
      661 gccgggtctt atctcgaaat cttaattttg agccttatgt ggcacaaaga tgcaaagctg
      721 ctcagagagt ttgcggcagt ctccgaacga tttacctata atccgaattt ttcgcttaat
      781 gttgagtatg tcacttcggt atcaaccgga gagtatatca ccgaggacga agatgcggtc
      841 gcgcctgacg aaaatctgct cagggatatt aaaagacgga cttcatacgt atacggatat
      901 tctcgtcttt ccggatatgc cgcaaaaatg tctgcgtctt cagtcgaaaa cagctcgcgc
      961 agtcttgatt ggtattttac agccaagcct gactttctgt gtaaaaatga tatgactcct
     1021 gcgatgcgcg gaacggcagc acaccatttt ctcgaagtgt gtgatttgga tatgggtgca
     1081 cagtccgtgg aaaaagaaac aaaaaggctt gtttctctcg gtaaattgac ggaagtcgag
     1141 gcggcggcag cagacaaaaa aatgcttgag cgattttttg aaagcaattt gttcaagcga
     1201 atcaagaatg cagataagat atacagagag caaaaattta caattgccgt tccgctcggc
     1261 gaattaaaca gcgatgtacc taaagagttc tcgtccgaaa aggcagttgt ccaaggcgtt
     1321 atcgactgcg ctttctttga aaacggagag acgattgttc ttgattacaa gaccgataac
     1381 gtgaaagatg aggaagagtt aaagcgcaga tattccgggc agctttcgat ttataagcgt
     1441 gccgcagagg agattttttc acagcccgtc aaggaaacgc t
//
LOCUS       DBMDVO010000384         1479 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41034_length_1479_cov_0.936865, whole genome shotgun sequence.
ACCESSION   DBMDVO010000384 DBMDVO010000000
VERSION     DBMDVO010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41034_length_1479_cov_0.936865"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1479
                     /locus_tag="ACZPE2_05800"
     CDS             <1..>1479
                     /locus_tag="ACZPE2_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1319396.1"
                     /translation="QNLCDLLGVRREELLDDSRDLYALRVHPADREKYSDFIQRIIMN
                     EQPLAGEYRLVKKDGTIICVRDTVTPRRLDDGTLTGHSVLTDITNIKNENTDLQFLNE
                     TVPCGFLRYTCEKQPRITYINPKMIELLRFPEVKDGEIDYQEMYKSNIFLMIPMEERR
                     RFSKYLNRVFSVEAPIAGDMTLLRCDGTRAHVFGWVTKCINEQGEAEFQSVCMDVTER
                     YQARKTSEGKRYLQALTDVYDKIFEFNLDANTVKCLHCEKASYFKRFENIAIQIEDAL
                     EKWLIDSVNAEQQDAVRRFFKDFCQKRLSGIDGKPPQITYKARSTDGNIKQYTGLFIK
                     VDESVSFYCCRETKEITDSVALREENNQLKEKMRDLVTHFSDGIAAFEVTAEGLVKPL
                     YATENVCEFFGYTEEEWLPLTERFTPVENFVAYSEVAYENFAELLRTGEAEFAYFDYQ
                     SETERKIKAICSEKEPNANSSRYVMLYPVEGGSEVVKQTLPEN"
BASE COUNT          453 a          285 c          355 g          386 t
ORIGIN      
        1 cagaatctgt gtgatttgct cggtgttcgc agagaagaac tgttggatga cagcagggat
       61 ctatatgcac ttcgagttca cccggctgat cgcgaaaagt attccgactt tattcaaaga
      121 ataattatga atgaacaacc tcttgccggt gagtaccgcc ttgtaaagaa ggacggtaca
      181 attatctgtg tgagagatac cgttacgccc aggagattgg atgacggaac actcaccgga
      241 cattctgttt tgacagacat aaccaatatt aaaaacgaaa ataccgatct gcaatttttg
      301 aacgaaactg ttccgtgcgg atttttaaga tatacttgtg aaaagcaacc gaggattaca
      361 tatatcaatc ctaagatgat tgagcttctg cgtttccccg aagtgaaaga cggagaaata
      421 gattatcagg aaatgtataa aagcaatatc tttttgatga ttccgatgga agaacgccgc
      481 agattttcaa aatatctgaa tcgggttttc tctgtggagg ctcctatcgc cggagatatg
      541 accttgcttc ggtgtgacgg aacccgtgca catgttttcg gatgggttac aaaatgcatc
      601 aacgagcagg gcgaagcaga gttccaaagc gtttgtatgg atgttacgga acgctatcag
      661 gcacgaaaga cgagcgaagg caaacgctat ctgcaagccc ttaccgatgt ttatgataag
      721 atatttgagt tcaacctgga tgccaatacc gtaaaatgcc tgcattgtga gaaagcatcc
      781 tattttaagc ggtttgaaaa tatagcaata cagattgaag atgcattgga aaaatggctc
      841 atcgattctg taaacgcgga acaacaagac gctgttcgtc gattttttaa ggatttctgc
      901 caaaagaggc tgagcggaat tgacggcaag ccgccgcaga tcacctacaa agctcgttct
      961 accgatggta acatcaagca atatacaggc ctatttatta aagttgatga atcggttagc
     1021 ttctattgtt gccgtgagac aaaagagata acggattctg tggcattgag ggaggaaaac
     1081 aatcagttga aagaaaaaat gcgtgattta gtcacgcatt tttccgacgg tattgcagcc
     1141 tttgaagtta cagcggaagg tctggtaaag ccgctgtatg ccaccgaaaa tgtttgcgaa
     1201 ttcttcggct ataccgagga agaatggctg ccgttgaccg agcgatttac ccccgtagag
     1261 aactttgttg cgtatagcga agtagcttat gagaattttg cggaattgct gagaacggga
     1321 gaagctgaat ttgcttattt tgactatcag tctgaaacag agcgcaaaat taaagcaatc
     1381 tgttccgaga aagaaccgaa tgccaattct tcacgctatg ttatgctcta tccggtagaa
     1441 ggcggctcag aggtcgtgaa acaaacctta cccgaaaat
//
LOCUS       DBMDVO010000385         1476 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41155_length_1476_cov_1.128000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000385 DBMDVO010000000
VERSION     DBMDVO010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41155_length_1476_cov_1.128000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(261..761)
                     /gene="ispF"
                     /locus_tag="ACZPE2_05805"
     CDS             complement(261..761)
                     /gene="ispF"
                     /locus_tag="ACZPE2_05805"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1319397.1"
                     /translation="MNYRIGHGYDVHKLQKGRELILGGVHIENGGIGLLGHSDADVLA
                     HAVCDALLGAAALGDIGKHFPDTDEAYKNADSMVLLSKVCDLLKKNGYSVGNIDATVL
                     AQSPKLAPYIAAMRTNIAKACKIPLSDVSVKATTEEGLGFTGAKEGIAAHSVCIIIKE
                     KEIKFI"
     gene            complement(751..>1476)
                     /gene="ispD"
                     /locus_tag="ACZPE2_05810"
     CDS             complement(751..>1476)
                     /gene="ispD"
                     /locus_tag="ACZPE2_05810"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1319398.1"
                     /translation="TKKYNNEAASYPYVSAIIVAAGNSTRMGGVNKQFLLIDGVPVLI
                     RTLKAFENSDLIKEIIISAREEDIPQIFAMVKDYSVNKVSNIVKGGCTRQESVFNAVR
                     CCMTESDYIAIHDGARPLVDRETIEKTIFAAFDFNAAAPGIPVKDTIKTVSDEGIVTS
                     TPARDSLRAIQTPQVFNKKMYLNAMQGVPNSELFTDDCGLIEAYGKLVKIVDGDNTNI
                     KITTPEDLIIAEAIINKGEKDEL"
BASE COUNT          424 a          329 c          239 g          484 t
ORIGIN      
        1 tcataaacga gtggttttag ctataaatgt gaatattact gaatatgcct aacatcattg
       61 cgataacact taaaacaatc agaattatca aaattaccat aagaaaacgc agatattttt
      121 ttacgttttt atcgtccgga gatgttgcat tttctctttt ggttttttta taaagaattt
      181 ttaataaaac agcgctgaat aaaacacaac atattatggt aaagttcaat atcaacatca
      241 atgttttttg agtttgcata ttatataaat tttatctcct tctcttttat tataatacac
      301 acactatgcg ccgcaattcc ttcctttgct cctgtaaagc caagcccctc ttctgttgtc
      361 gcttttacgc tgacatcaga caacggtatt ttacatgctt ttgcaatgtt tgtgcgcatc
      421 gctgcaatat acggcgcaag cttcgggctt tgagccaaaa ccgtggcatc tatatttccg
      481 acagaataac cgttcttctt aagcaaatca cacactttag ataaaagaac catactgtcg
      541 gcatttttat aggcttcatc tgtatctgga aaatgttttc cgatatcacc gagcgcagct
      601 gccccgagta aagcgtcaca gacagcgtgg gcaagtacat ctgcgtctga atggcctaaa
      661 agaccaatcc caccattttc aatatgaact ccgcctaata ttagttctct acccttttga
      721 agcttatgaa catcatatcc atgcccgatt ctataattca tctttttctc ctttgttgat
      781 aattgcttcg gcaatgatta aatcctcagg agtcgtaatt ttgatgtttg tattatctcc
      841 atcaacaatt ttgacaagtt taccgtatgc ttcgattaag ccgcaatcat ccgtaaaaag
      901 ttcactgttc ggaacgccct gcatagcgtt taagtacatt tttttattaa atacctgcgg
      961 cgtttgaatt gcccttagcg aatccctcgc cggcgttgaa gttactattc cttcatcact
     1021 aacggttttt attgtatctt taaccgggat accgggcgca gcggcattaa aatcaaaagc
     1081 cgcaaaaatc gttttttcaa tcgtttctct gtccacaaga ggccttgcac cgtcgtgtat
     1141 ggcaatataa tcggactctg tcatacaaca cctaacagca ttaaaaacgc tttcctggcg
     1201 cgtacagccg cctttaacga tattacttac cttattaacc gaataatctt ttaccatagc
     1261 aaaaatttga ggaatgtcct cttcgcgtgc cgatataatt atttccttaa taagatccga
     1321 attttcaaat gccttcaaag tcctgataag cacagggacg ccgtcaatta gcaaaaactg
     1381 cttattgacg ccgcccattc ttgttgaatt tccggcagct acaataatcg cggaaacata
     1441 cggataagaa gctgcctcat tattatattt ttttgt
//
LOCUS       DBMDVO010000386         1476 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41163_length_1476_cov_0.915636, whole genome shotgun sequence.
ACCESSION   DBMDVO010000386 DBMDVO010000000
VERSION     DBMDVO010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41163_length_1476_cov_0.915636"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..578
                     /gene="ybeY"
                     /locus_tag="ACZPE2_05815"
     CDS             60..578
                     /gene="ybeY"
                     /locus_tag="ACZPE2_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1319399.1"
                     /translation="MTDKIKVIISNKQKTVKIPTGVRMLVRRCCNAVLAMEQFEGSAE
                     ISVSFVDDAEIRELNKKHRNIDASTDVLSFPLGENGVYDINHDTGAKMLGDIVISIEH
                     AVAQAEEYGHSLQREIAFLTVHSLLHLLGYDHVNGGLEAVHMREKEETVLTQLGLKRN
                     GSYYIDQSEDKI"
     gene            578..1081
                     /locus_tag="ACZPE2_05820"
     CDS             578..1081
                     /locus_tag="ACZPE2_05820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR1319400.1"
                     /translation="MRGLIRSFGYAFRGIFYTLKHERNMRIHFVCMIYMYSFLLMADF
                     FEITRTQFAIIFLANALVVSLELVNTAVERTVDLASTEWTDNGRAAKDTAAGAVLVSA
                     IFAVLTGIMIMWQPKAFSALYVYFKEHILYFVLFLLSLVVAFIFIFKGFPQIKKKSSD
                     RADKEKK"
     gene            1078..>1476
                     /gene="era"
                     /locus_tag="ACZPE2_05825"
     CDS             1078..>1476
                     /gene="era"
                     /locus_tag="ACZPE2_05825"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1319401.1"
                     /translation="MNESKSAFAAIVGKANVGKSSILNRLIGSKIAIVSGKPQTTRTK
                     IMGVLTTDDNVQLVFTDTPGFFKPKDKLGEYMVEAVNDSISGVEECLFVVDATDKELN
                     AAEKELINKFRSAKMPVITALNKIDMISRKE"
BASE COUNT          455 a          287 c          340 g          394 t
ORIGIN      
        1 aggcataaat tagtacagga tataatcata gcatatgaga aaaatgagga gaaaagaaaa
       61 tgacagacaa aattaaggta atcatctcaa acaaacagaa aacagtaaaa atacctacgg
      121 gtgtgcgtat gctcgttcgt cgctgctgca atgcggtact cgcaatggag caatttgaag
      181 gctccgccga aatcagcgtg tccttcgtgg acgacgccga gataagagag cttaacaaaa
      241 aacaccgtaa tatcgacgcg tcaacagatg ttttgtcttt tccgctcggt gaaaacggcg
      301 tttacgatat aaatcatgac acaggtgcaa aaatgctcgg cgacatagtt atatcaatcg
      361 agcatgcagt agctcaagca gaggaatacg gacactctct tcagagggaa atcgcgtttt
      421 tgaccgtcca ttctcttttg catcttcttg gttatgacca tgtgaacggc ggacttgagg
      481 cggtacatat gcgtgaaaaa gaggagactg tgttaacaca gctcggtctc aaacgcaacg
      541 gcagctatta tatagaccag agtgaggata aaatctgatg cgcgggctca taaggagctt
      601 cggctatgct ttccgcggaa tattttacac acttaaacac gagcgcaata tgagaataca
      661 ttttgtctgt atgatatata tgtattcttt tttgctgatg gcggactttt ttgagattac
      721 aagaactcag tttgcgataa tatttcttgc aaacgcactt gttgtgtctc ttgagcttgt
      781 caacaccgca gttgaaagaa ccgtcgattt ggcaagcacc gaatggactg ataacggcag
      841 ggcggcaaag gataccgcgg caggagccgt tttggtatcg gcaatatttg ccgttctcac
      901 gggaattatg ataatgtggc agccaaaggc cttttcggca ctttatgttt attttaagga
      961 gcacatatta tattttgtgc tgtttctttt aagtcttgtt gtcgctttta tttttatatt
     1021 taaaggtttt ccgcaaatta agaaaaaatc ttccgacaga gcggataagg agaaaaaatg
     1081 aacgaatcaa aatcagcttt tgcagccata gtcggcaagg caaacgtcgg caaatcttcg
     1141 atactcaacc gacttatagg cagtaaaata gccatagtat ccggtaaacc tcagaccacc
     1201 cgtacaaaaa ttatgggcgt tttgactacc gatgataatg tgcagcttgt ttttaccgac
     1261 actccgggat tttttaaacc taaggacaag ctcggcgaat atatggttga ggcggtaaac
     1321 gacagcatat ccggcgttga agagtgcctg tttgtcgtcg atgcgacgga taaagagctt
     1381 aacgccgcgg aaaaggagct tataaataaa tttcgctcgg caaaaatgcc tgttataaca
     1441 gctcttaaca aaatagatat gataagccgt aaggaa
//
LOCUS       DBMDVO010000387         1474 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41211_length_1474_cov_1.672979, whole genome shotgun sequence.
ACCESSION   DBMDVO010000387 DBMDVO010000000
VERSION     DBMDVO010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41211_length_1474_cov_1.672979"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(204..461)
                     /locus_tag="ACZPE2_05830"
     CDS             complement(204..461)
                     /locus_tag="ACZPE2_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319402.1"
                     /translation="MNGLNGKPTEYPENALVPYPVILAASKGDPDAMKIVLQHFSGYI
                     ASLSMRKLYDERGNVYFGVDEEIRERLQAKLMRAILTFRAE"
     gene            complement(442..882)
                     /locus_tag="ACZPE2_05835"
     CDS             complement(442..882)
                     /locus_tag="ACZPE2_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1319403.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVTFS
                     NLALHEERQLYTVDKYFQDEEAEPSYQMAGKEITPKLLLEAIRALPEERRKIILLYYF
                     EGLTDIEIGKQLNISRSTVQYRRTSSFEQLKKYLEENADEWIEW"
BASE COUNT          379 a          337 c          302 g          456 t
ORIGIN      
        1 ttgcggtgtg gtatttctcg ctcgcttttc ggttaagaaa aaggtcatgg cgtacccacc
       61 gcatggctcg gcacaaaagg aacgtatccg atttgcctta tactgcgtcg ccgttctatt
      121 gcgccgcttt ctttgtcaaa gagcagagag ctgttctgcg atatggaacg gaaaagggag
      181 aagcgttcca ataccgcgtt ggtttattca gccctaaatg tgaggattgc cctcatcagt
      241 tttgcttgca gcctttcgcg tatttcttcg tctactccaa aataaacatt tcctcgctca
      301 tcatagagct tccgcatgga aagacttgct atatagccgc tgaaatgctg caatacaatc
      361 ttcattgcgt ccgggtcgcc tttgctcgcc gccaaaataa cgggataggg aacaagggca
      421 ttttccgggt actcggtcgg tttaccattc aatccattca tcagcgtttt cctccaaata
      481 tttttttaac tgctcaaagg agcttgtccg gcgatactgt accgtacttc ttgagatatt
      541 gagttgcttt ccgatttcta tatcagtcaa tccctcaaag taatacagca gtataatttt
      601 gcgccgttct tcgggcaaag cccggatagc ttcaagtagc agttttgggg ttatttcttt
      661 tcctgccatt tgataagacg gctctgcttc ttcgtcttga aaatacttat cgacggtata
      721 aagttgccgt tcctcatgca aggcaaggtt ggaaaaagta acctcccgaa ctctgcgccg
      781 acgaatttct tcatgggcgt tgcacgcttc gttgtgcagc actgatttac agaacttttg
      841 aaacgcacat tgcttttcaa attccctgcg attaggtgtc atggtgttca cctcctttct
      901 gccgaaaggc ggtagtacct ccccttttcg cgggataata caggcgactt tttgctttgc
      961 caaaaagagg acaaagtttt tgaaaaaatc ttctgtaaac gcaaaatacg cccgcccgaa
     1021 aaaactcgga caggcgcaga ggaattaaaa gaagtattca gatgatttga caattcatat
     1081 atatagccgc aataacccct tgtaggaggc gggctttttt tgataaggta ttccttatct
     1141 gtatgtattc attctttcct tttttcacaa tgacaacctc ccaatatacc gcccctgctg
     1201 attgcatggg atagagagtg tcaaagtgct ttaagcaaaa agaaacggcg gcttttccat
     1261 aagccaccgt ttcaaaggcg cgtgaaaata tgtctgcttt acgacggctt tttttctttt
     1321 gccgtcgcgc ggcagattta gtctatttta tatccatttg ttataatatt tgcaatatct
     1381 aaatttattc tctgtggtta ttcagtcact tttttcgcct taccaaaaac aaaacacaag
     1441 caagagaaaa tacaatacta catataatgt acgt
//
LOCUS       DBMDVO010000388         1474 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41227_length_1474_cov_1.152950, whole genome shotgun sequence.
ACCESSION   DBMDVO010000388 DBMDVO010000000
VERSION     DBMDVO010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41227_length_1474_cov_1.152950"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>532)
                     /locus_tag="ACZPE2_05840"
     CDS             complement(<1..>532)
                     /locus_tag="ACZPE2_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR1319404.1"
                     /translation="LLAILAATIAVPAIIGHDMSQSAALFGAGIGTIVYLLFTKFRSP
                     VFLGSSFAFIGSMLAAFAGSISMQLGYLGLILGAFFAGLVYVVIAIAVKISGVKWINK
                     LMPAVVIGPTVSIIGLSLAGNAISDLTQGKVMADSVEQVVENGTIVDKVTQVSTASPY
                     VALICGLVTLFTVILCS"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(658..1194)
                     /gene="pyrR"
                     /locus_tag="ACZPE2_05845"
     CDS             complement(658..1194)
                     /gene="pyrR"
                     /locus_tag="ACZPE2_05845"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR1319405.1"
                     /translation="MSSKIIIDTQTLTRTLARLAHEIIEHSEGYEEVYLVGIKRRGVP
                     LAKMLKTNIESFSNLKVHLGELDITLYRDDLSEKYSSPQLNGTQIDFDVTNKNIILVD
                     DVLFTGRTARAAMDAIIKLGRPAKIQLAVIIDRGHRELPICANYVGKNIPTSSDEFVS
                     VKVNEIDGAFRAELYKNS"
BASE COUNT          429 a          302 c          297 g          436 t
ORIGIN      
        1 gagcaaagaa taacggtgaa aagggtgaca agtccgcaaa taagtgcaac atacgggctt
       61 gcggtcgata cttgagtcac cttatcaaca atagtaccgt tttcaacaac ctgctctacc
      121 gagtctgcca taaccttgcc ctgtgtaagg tcggaaatag cattgccggc aagcgaaaga
      181 cctatgatcg aaactgtggg accgatgaca actgccggca taagcttgtt aatccatttt
      241 acgccggaaa tctttacggc aatggcaatt acaacataaa caaggcctgc gaagaatgca
      301 cctaatataa ggcctaaata tccaagctgc attgagatgc tgcctgcaaa cgccgcgagc
      361 attgatccta tgaatgcaaa tgaagaacca aggaaaaccg ggcttctgaa cttcgtgaag
      421 agaaggtata cgattgtgcc tatgccggcg ccgaaaagtg ctgccgattg gctcatatcg
      481 tgaccgatta tcgccggtac tgcaattgta gctgcaagaa tcgcaagcaa ctnnnnnnnn
      541 nngaaatgca aaaacgatta cttgcccgaa tttgggtttg tctttgatgt cgtaaataag
      601 tttcatttac tacgcctcct gaatatattt aggctcggat aaaatcacga gaccttatta
      661 actgtttttg taaagctcag ctctgaatgc tccgtcaatt tcatttacct taacacttac
      721 gaactcatca cttgatgtag gtatgttttt accgacgtag tttgcacata ttggtagttc
      781 tctgtggcct cggtctatta tgactgcaag ttgaatttta gctggtctgc cgagcttgat
      841 tattgcatcc attgcggctc ttgctgtgcg accggtaaaa agcacatcgt ccaccaaaat
      901 gatattttta tttgttacat caaaatcaat ttgagtaccg tttagctgtg gagaggaata
      961 tttttctgac aaatcatctc tgtataaagt tatatcaagt tcgccgaggt gcactttcaa
     1021 atttgaaaaa ctctcaatat tagttttgag cattttagca agcggtacgc cgcgtcgttt
     1081 aattcccaca agatagacct cttcatagcc ttcactgtgt tcaattattt catgagcaag
     1141 gcgggctaat gttcttgtca aagtttgggt gtctataatt atctttgagc tcatctttaa
     1201 taccatccgc gcaacaaaaa ataccttgac gcaatgcgac aaggtatacc tacacaaata
     1261 tttcttcgcg ttaccttgtc ggcatctcgt accgaattaa aggactgctt tactttttgg
     1321 tataatacca tatcaaagtg taaaagtaaa taggaaaaag tcgttttttg aaactttgtg
     1381 taaacctatt ttgcttttgg cctttatgcg cgattgacaa aaccgatgtg tgtaattata
     1441 attaaaggac tttaaataat catttgaacg ggag
//
LOCUS       DBMDVO010000389         1469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41436_length_1469_cov_1.055556, whole genome shotgun sequence.
ACCESSION   DBMDVO010000389 DBMDVO010000000
VERSION     DBMDVO010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41436_length_1469_cov_1.055556"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /gene="truB"
                     /locus_tag="ACZPE2_05850"
     CDS             complement(<1..160)
                     /gene="truB"
                     /locus_tag="ACZPE2_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511476.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1319406.1"
                     /translation="MTGFICIDKPENMTSFTAANLVKRITGSKKAGHTGTLDPMATGV
                     LTVALSGAT"
     gene            complement(157..1131)
                     /locus_tag="ACZPE2_05855"
     CDS             complement(157..1131)
                     /locus_tag="ACZPE2_05855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1319407.1"
                     /translation="MKRINEAEAAEILKSKDNILILTHRSPDGDTLGCAFALRRVLFG
                     MGKTVMVDCSDDIPKKYSYMWGESEENYNPQKSMDFSPDFVLAVDVADSKLLGARLTE
                     KYPEIPLCIDHHISNTGYADMLCLKDSAAACEIVYDVIKCLGVKPDACTADCLYTGIS
                     TDTGCFRYSNVTPETHVKAAELISLGAQFDEINRVMFETKTRTYLRLEELVLNSIEMY
                     FGGKCAVITITKDMFKQSGSNETECDGIASLPRKIEGVKVGVTLRERADGTFKVSLRT
                     YAPVDASAICAKMGGGGHARAAGCDFSADYADGKKQLLEIIKTELEKA"
     gene            complement(1115..>1469)
                     /gene="rbfA"
                     /locus_tag="ACZPE2_05860"
     CDS             complement(1115..>1469)
                     /gene="rbfA"
                     /locus_tag="ACZPE2_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1319408.1"
                     /translation="YRKDRVAEDIKREIVTLIRELKDPRVKDKMLTVVRVEVSQDLSY
                     AKVFVSSLSGIDDAKQAVKGLESATGLIRHEVGKRLRLRKAPELKFAADDSVEHGMEI
                     FRKIEALKKDDEEDK"
BASE COUNT          335 a          411 c          314 g          409 t
ORIGIN      
        1 gcgtcgcccc cgagagggca acagtcaaca cgcccgttgc catagggtcg agcgtgcctg
       61 tatgcccggc ttttttggag ccggttattc tctttaccaa atttgccgcc gtaaacgacg
      121 tcatattttc aggcttgtca atgcagataa atcccgtcat gctttttcaa gctccgtttt
      181 gattatttca agaagctgct ttttgccgtc ggcataatcg gccgaaaaat cacagcccgc
      241 cgcacgcgcg tgacctccgc cgcccatctt ggcgcaaata gccgaggcgt ctacgggagc
      301 gtaggttctg agcgatacct taaacgtgcc gtcagcccgt tctctaagcg taacgcctac
      361 cttaacgccc tcgattttac gcggaagaga agctatgccg tcacactcgg tctcgtttga
      421 gccgctctgt ttaaacatat ccttggtgat tgtgataacg gcgcatttcc caccgaagta
      481 catttctatc gaatttaaaa cgagctcctc aagccgcaaa taagttctgg tctttgtctc
      541 aaacataact cggtttatct cgtcaaattg tgctccgagc gaaatgagct ccgctgcctt
      601 aacgtgagtt tcgggagtta cgtttgaata cctgaagcag cctgtgtccg ttgaaattcc
      661 cgtatacaga cagtctgccg tgcaagcgtc cggttttact cccaggcact ttattacgtc
      721 ataaactatc tcgcacgccg ccgcactgtc tttaagacag agcatatcgg catatcccgt
      781 atttgaaata tgatggtcta tgcacagcgg tatttcgggg tatttttccg taagacgcgc
      841 gccgagcagc tttgaatcgg caacatcaac cgccaacaca aaatcgggag aaaaatccat
      901 actcttttgc ggattgtaat tttcctccga ctctccccac atatacgaat acttcttagg
      961 tatgtcgtcg gagcaatcga ccataaccgt tttgcccatc ccgaagagaa cgcgtcggag
     1021 cgcaaaagca caaccgagcg tgtcgccgtc gggactgcgg tgcgtcaaaa tcagaatgtt
     1081 gtctttactt ttgagaattt ctgccgcttc ggcctcattt atcctcttca tcgtcttttt
     1141 tcagagcctc aatctttctg aaaatttcca taccgtgctc aaccgaatca tctgccgcaa
     1201 atttaagctc gggagccttt ctcaaacgaa gtcgtttccc gacctcgtgc cgtataagcc
     1261 ccgttgcgct ctcaagcccc tttacagcct gctttgcgtc atctatgccg gagagtgagc
     1321 ttacaaaaac ctttgcatag ctcaaatcct gacttacctc aacccttacc acggtgagca
     1381 ttttatcttt tacgcgcgga tccttgagtt cgcggataag cgtaactatt tcgcgcttaa
     1441 tatcctccgc gactctgtct tttctgtat
//
LOCUS       DBMDVO010000390         1466 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41553_length_1466_cov_1.047619, whole genome shotgun sequence.
ACCESSION   DBMDVO010000390 DBMDVO010000000
VERSION     DBMDVO010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41553_length_1466_cov_1.047619"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="ACZPE2_05865"
     CDS             complement(<1..847)
                     /locus_tag="ACZPE2_05865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1319409.1"
                     /translation="MNLIFLGAAHEVTGSCTLLTACGKNILIDCGMEQGPDTYKNRDI
                     PVSPADIDCIFLTHAHIDHSGKLPYLTANGYKGPIYATASTAKLCRIMLEDSAHIQQF
                     EAQWRNRRAKRSGESGYVPLYTVEDAQKCCSQFLRCEYGVDYHVFDGVSISFTDAGHL
                     LGSSSITINVTENGETEKIVFSGDIGNTDQPIIRDPQYITDATYVITESTYGDRSHGE
                     RLDYISQLVPILDRTFARGGNVVIPSFAVGRTQELLYFIRQIKEERMLKNYNDFPVYV
                     DSPLAV"
     gene            complement(1020..>1466)
                     /locus_tag="ACZPE2_05870"
     CDS             complement(1020..>1466)
                     /locus_tag="ACZPE2_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319410.1"
                     /translation="GVGFDVVYQGLFVTLLTLAAYFIGHFMEAGRWELTESADGMTMA
                     FLTMSMCEIFHSFNMRSQHGSTVSMLLHGSFNKYIFGSTVLSLITTALVIEVPFLADA
                     FDFTTIDAREFFTALGLAFLIIPLVEIVKAIERAVIKNKAKKQTVK"
BASE COUNT          363 a          333 c          370 g          400 t
ORIGIN      
        1 caactgcaag gggactgtcc acatatacgg ggaagtcgtt gtagtttttg agcatacgct
       61 cttcctttat ctgccttata aagtataaaa gctcctgagt tctgccgacg gcaaacgacg
      121 gtataactac attgccgccg cgcgcaaagg tgcggtcaag tatgggcacg agctgcgata
      181 tgtagtcgag tctttcgccg tgactgcggt cgccgtaggt gctttcggtt atgacgtagg
      241 tggcgtcggt aatatactgc gggtcgcgta taatcggctg gtcggtattg cctatgtcgc
      301 cggaaaaaac tattttttcg gtttctccgt tctcggtgac gtttattgta atgctcgatg
      361 agcccaaaag atgacccgca tcggtaaagc ttatggatac accgtcaaaa acgtgataat
      421 ccacaccgta ttcgcatctc aaaaactgag aacagcattt ttgagcgtcc tctacggtgt
      481 agagcggaac atatcccgat tcgcccgagc gttttgccct tcggttgcgc cattgtgctt
      541 caaactgctg tatgtgcgca gaatcctcaa gcataattct gcagagcttc gctgtggacg
      601 cggttgcgta aatcggccct ttgtatccgt ttgccgtaag gtacggcagc ttgccggaat
      661 ggtctatgtg agcatgggtc agaaaaatgc agtctatatc ggctggagat acgggaatat
      721 cccggttttt atatgtgtcg ggtccttgct ccataccgca gtcaatcaaa atgttcttgc
      781 cgcaggcggt gagaagcgtg cagcttcccg tgacttcgtg tgcggctcct aagaatataa
      841 gattcatatt ttccgctcct ttttaaatgg tggcgcccat cgccgatgcc ttttttatta
      901 tggtaacata aatcatcgca aaaatccagt attaagccgc tgcgggataa ataaaacggc
      961 acggcagtag ttcttactgc cgtgccttga aaaacatgta tttgttttgt cgccaaagct
     1021 tattttacgg tctgcttttt ggctttgttc tttataacgg cacgctctat tgccttgaca
     1081 atttcaacga gaggaattat caggaacgca agaccgagag ccgtgaagaa ttcgcgagca
     1141 tcaatagtgg tgaaatcaaa agcgtctgca aggaacggta cttctataac gagcgcagtt
     1201 gttatgaggg aaagtacggt tgacccaaaa atgtatttgt tgaagctgcc gtgaaggagc
     1261 attgatacgg ttgagccatg ctgcgaacgc atattaaacg agtggaatat ttcgcacatt
     1321 gacattgtaa ggaatgccat ggtcataccg tcggcactct cggtaagctc ccatctgcct
     1381 gcctccataa agtggcctat gaagtaagcg gcaagtgtca gaagcgttac gaaaagccct
     1441 tggtatacta cgtcaaagcc gacgcc
//
LOCUS       DBMDVO010000391         1466 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41560_length_1466_cov_0.886447, whole genome shotgun sequence.
ACCESSION   DBMDVO010000391 DBMDVO010000000
VERSION     DBMDVO010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41560_length_1466_cov_0.886447"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>777)
                     /locus_tag="ACZPE2_05875"
     CDS             complement(<1..>777)
                     /locus_tag="ACZPE2_05875"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1319411.1"
                     /translation="TYDAKAGYSLVLTIDEVIQYYLEQSLDQALTDTGAKYAYGIIMD
                     VKTGAILGMTSKPDFDLNSPNKISNKVLAESISAISSPDEQKKATTDALYAQWRNRDI
                     SDTYEPGSVFKTIVVSAALEEGVVDLNTTYTCTGGIQVANNYQKCWKPGGHGTENLTQ
                     GLMNSCNPFFVTIGQKLGKDTFYKYFEAFGFTEKTGIDLPGEAGTIMHKKENMGLVEL
                     ATVSFGQSFQITPIQLITTAASIVNGGTRITPHFGVKAVA"
     assembly_gap    778..787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<788..1430)
                     /locus_tag="ACZPE2_05880"
     CDS             complement(<788..1430)
                     /locus_tag="ACZPE2_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015662.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1319412.1"
                     /translation="MQTGPSKRMGKRAVTVLCFIAAAFLVCVGSLAYISLIHGNEYKL
                     KAEQNQLSDTTVSAQRGTIYDSNMKALAKSASAWLVYINPSKITSDEQRDIVVTNLST
                     MLGVDEETVRKKAERTSSGYEKIAGEVETDVKDRLKTFISENKLSSIIGVDPDTKRYY
                     PYSSFASTIIGFTDSDDSGRLGLELKYNDELTGTAGRIITAKNARQGSMSTDYQ"
BASE COUNT          347 a          360 c          330 g          419 t
ORIGIN      
        1 ccgccacagc ctttactccg aaatggggcg tgatccgtgt tccgccattc acaatggaag
       61 ccgcagtggt aatcagctgg ataggcgtga tctgaaaaga ctggccaaag gaaacggtag
      121 ccagttccac aagccccata ttttcctttt tatgcatgat ggttcctgcc tctcccggca
      181 ggtcgatgcc cgttttttcg gtaaaaccga acgcttcaaa atatttataa aaagtatctt
      241 taccgagctt ttgccctatt gtcacaaaga acgggttaca ggaattcata agtccctgcg
      301 tcaaattttc cgtaccgtgg ccgcccggct tccagcactt ctgataatta ttcgcaacct
      361 gaataccgcc ggtgcaggta tatgttgtat tgaggtccac aacaccctct tcaagagccg
      421 ccgaaacgac tatcgtctta aataccgaac ccggctcata tgtatcgctt atatcgcggt
      481 ttcgccactg agcataaaga gcgtccgtcg tagccttttt ttgctcgtcg ggagaggata
      541 ttgcggatat gctctcggca agcaccttgt tagaaatctt attaggagaa ttcaaatcaa
      601 agtcgggctt tgacgtcata ccgagtatag cgccggtttt gacgtccatt attatgccgt
      661 aagcatattt cgcgcccgtg tccgtaagtg cctggtcgag cgactgctcg aggtaatact
      721 gtattacctc gtctattgta agcacaaggc tgtagcctgc cttagcgtca tatgtggnnn
      781 nnnnnnnttt gataatccgt ggacatagag ccctgtctgg catttttagc cgttattatt
      841 ctgccggcgg tgccggtaag ctcgtcgttg tatttcagct caagcccgag cctgcccgaa
      901 tcatcggaat ccgtaaagcc tattattgtg gaagcaaacg acgagtaagg ataatatctc
      961 tttgtatcgg gatcaacacc gattattgag gaaagcttgt tttcggaaat aaacgtttta
     1021 agacggtcct taacatccgt ctcaacctcg ccggctattt tttcgtagcc gctgcttgtg
     1081 cgctccgcct ttttgcgaac cgtctcttcg tccacaccga gcatggtact gagatttgta
     1141 accacaatgt cgcgctgctc gtcgcttgtt atctttgacg gatttatata gacaagccac
     1201 gccgaagcac ttttggcaag agctttcata tttgaatcgt aaatcgttcc gcgctgagcc
     1261 gaaaccgtgg tgtccgaaag ctgattttgc tcggctttaa gcttatattc gttgccgtga
     1321 ataagagaaa tatatgcgag cgagccgaca catacaagaa aagctgcggc gatgaagcac
     1381 agcaccgtaa cagctctttt acccattctc tttgaaggtc cggtctgcat atttttctcc
     1441 tttcattaaa tttaagtctg attttg
//
LOCUS       DBMDVO010000392         1464 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41643_length_1464_cov_1.035950, whole genome shotgun sequence.
ACCESSION   DBMDVO010000392 DBMDVO010000000
VERSION     DBMDVO010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41643_length_1464_cov_1.035950"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            24..1307
                     /locus_tag="ACZPE2_05885"
     CDS             24..1307
                     /locus_tag="ACZPE2_05885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HIR1319413.1"
                     /translation="MKDFIKKPPMGWNSWDCYGAGVTEDELLGNAEFMRDRLKQYGYQ
                     YVVCDIQWYEPAAKGNVYNNFADLCMDEYSRLIPAVNRFPSSANGAGFKPIADKIHSM
                     GLKFGIHIMRGIPRQAVHRNTRIYGTTARARDIASQFSLCPWNTDMYGVDTEKRGAEE
                     YYDSLFKLYASWGVDFVKVDDIANTEFSPQNPYSAEKEIEMIRAAIDRSGRDMVLSLS
                     PGPAPLNKAEHLSENANMWRISGDFWDRWDKLLNMFSLCEKWYPYVKDGSFPDCDILP
                     LGKLCIDGSYMGDMGRDSGFTKEEQKTMMTLWAVFRSPLFFGGELRLTDKYTLSLVTN
                     PEVINVNQNSEKPLFVYNKGGIAVWQTKIENCTAVAVFNLSDEEKHYKLSFSDLGIEN
                     VRAVRDLWARKDIPKCENDVAVSLKPHSSEFFEIY"
     gene            1342..>1464
                     /locus_tag="ACZPE2_05890"
     CDS             1342..>1464
                     /locus_tag="ACZPE2_05890"
                     /inference="COORDINATES: protein motif:HMM:NF045064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319414.1"
                     /translation="MLNFIIGRNNSGKTEKIRALIAERASENKKCILIVPEQFSF"
BASE COUNT          413 a          294 c          361 g          396 t
ORIGIN      
        1 gcaaaagatt acagggtgaa gatatgaagg actttataaa aaaacctcct atgggttgga
       61 acagttggga ttgctacggt gcaggcgtaa ccgaagatga gcttcttgga aatgccgagt
      121 ttatgcgtga ccgattaaag caatacggct atcaatatgt tgtctgcgat atacagtggt
      181 atgagccggc ggctaagggc aatgtataca ataactttgc cgacctttgt atggacgaat
      241 attcgcggct tatcccagcg gtaaatcgct tcccgtcctc cgcaaacggc gcgggattta
      301 agccgattgc agataaaatt cactctatgg ggcttaaatt cggtatccac attatgcgag
      361 gtattccgcg tcaggcggtt caccgaaata cgcgtattta cggcacaact gcccgtgcgc
      421 gcgacattgc ttctcagttt tcgctgtgtc cttggaatac cgatatgtac ggggtggata
      481 cggaaaaaag aggggccgaa gaatattacg attctctttt taaactgtat gcctcttggg
      541 gcgttgactt tgtaaaagtc gatgacatag caaataccga attcagtccg caaaacccgt
      601 attccgcgga aaaagaaata gaaatgatac gtgccgcaat agaccgttcc ggcagagata
      661 tggtactgag cctgtcgccg ggtcccgcac cgttaaacaa agccgagcat ctctcggaaa
      721 atgcaaatat gtggcgtatt tcgggcgatt tttgggacag atgggataag cttctcaata
      781 tgttttcact ctgcgaaaag tggtatccgt atgtgaaaga cggctcattt cccgattgcg
      841 acattttgcc gctcggcaag ctctgcatag acggctctta tatgggcgat atggggcgtg
      901 attcggggtt tacaaaggaa gagcaaaaaa ccatgatgac cttatgggct gtttttcgct
      961 ctccgctttt tttcggcggc gagcttcgcc ttacggataa atatactctg tctcttgtca
     1021 caaatcccga agttataaat gtgaaccaaa actccgaaaa accgttgttt gtatataata
     1081 aaggcggcat tgccgtgtgg cagacaaaaa tcgaaaactg tactgcggtt gcggttttca
     1141 atctttccga tgaagaaaaa cattataaat taagtttttc ggatttgggt attgagaacg
     1201 tccgtgcggt tcgcgattta tgggcgagaa aagatattcc gaaatgtgaa aatgacgttg
     1261 cggtatcttt aaaaccgcat tcgtcggaat tttttgagat atattgattt ttgacattaa
     1321 cagcatagat gggggtgcaa aatgcttaat tttataatcg gtcgcaacaa cagcggcaaa
     1381 actgaaaaaa tcagagctct tatcgcagag agagcgtccg aaaataaaaa gtgtatactc
     1441 atagtgcccg agcagttctc gttt
//
LOCUS       DBMDVO010000393         1460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_41800_length_1460_cov_1.138337, whole genome shotgun sequence.
ACCESSION   DBMDVO010000393 DBMDVO010000000
VERSION     DBMDVO010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41800_length_1460_cov_1.138337"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            127..543
                     /locus_tag="ACZPE2_05895"
     CDS             127..543
                     /locus_tag="ACZPE2_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1319415.1"
                     /translation="MQEQRYSRQREAILENLKNRYDHPTAMELYLSVREDIPNLSLGT
                     LYRNLTLLRDSGKIISLTLGGEEHFDANTNQHYHFKCTSCGRYFDLPSEPLDSSVTAR
                     CLGDFKGRAEGYSLIFYGKCEECLKHLSVSNNKDNN"
     gene            563..1108
                     /locus_tag="ACZPE2_05900"
     CDS             563..1108
                     /locus_tag="ACZPE2_05900"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1319416.1"
                     /translation="MKKFVCPVCGYVYEGENPPEKCPQCGAPGSTFKEVKEESKSWAA
                     EHVVGIAKDVPEDILEGLRANFTGECSEVGMYLAMARVAHREGYPEIGLYWEKAAYEE
                     AEHAAKFAELLGEVITDSTKKNLEMRAEAENGATLGKFELAKRAKEEGLDAIHDTVHE
                     MARDEARHGRAFEGLLKRYFG"
     gene            1155..1313
                     /gene="rd"
                     /locus_tag="ACZPE2_05905"
     CDS             1155..1313
                     /gene="rd"
                     /locus_tag="ACZPE2_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0072592 - oxygen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR1319417.1"
                     /translation="MKKYACPCGYVYDPAVGDPDNGVAPGTAWEDVPEDWVCPVCGLG
                     KDSFTEED"
BASE COUNT          409 a          294 c          367 g          390 t
ORIGIN      
        1 taacgataat cgttactgat tattgagcgg ttacaaaccg caacgtaaaa cgcagttaaa
       61 gccgatgaat agacacggct ttttacggcg cacgttatcc gatatattaa gaacggaggc
      121 tgaatattgc aggaacagcg ttacagtcgg caaagggagg caatccttga aaatttaaaa
      181 aacaggtatg accacccgac tgccatggag ctttatctct ccgtaaggga ggatattccg
      241 aatttaagcc tcggtactct ctaccgcaat cttactcttc ttcgtgacag cggcaaaatc
      301 ataagcctta ccctcggcgg tgaggagcat tttgacgcta acacaaatca gcattatcat
      361 ttcaaatgca catcctgcgg cagatatttc gatttaccct ctgagccgct tgattcgtcc
      421 gttacggcac gttgcttggg tgatttcaag ggcagggcag agggttacag cctaatcttt
      481 tacggcaaat gcgaagaatg tttaaagcat ttgtccgtgt caaataataa agataataat
      541 taaattcttg gaggaattaa aaatgaaaaa atttgtatgt cccgtatgcg gttatgttta
      601 tgaaggcgaa aatccgcctg aaaagtgccc tcagtgcggc gctcccggtt caaccttcaa
      661 agaggttaaa gaggagtcaa aatcttgggc tgcagagcac gttgtaggta ttgcaaagga
      721 tgttcccgag gatatccttg agggtcttcg cgctaacttc acaggcgaat gctccgaggt
      781 cggtatgtat cttgcaatgg caagagtagc tcacagagag ggctaccccg aaatcggtct
      841 ttactgggaa aaggctgcct atgaagaggc agagcatgcc gctaaatttg cagagcttct
      901 cggtgaggtc atcacagaca gcaccaaaaa gaaccttgag atgagagccg aagcagagaa
      961 cggcgctaca ctcggtaagt ttgaacttgc taagagagct aaggaagagg gccttgacgc
     1021 tattcacgat accgttcatg agatggctcg tgacgaagcg agacacggca gagcctttga
     1081 gggtcttctc aagagatatt tcggttaatt ttttaagctt tgccaagtaa atatattgtt
     1141 aggaagtgtt tgctttgaaa aaatatgcat gcccatgtgg ctatgtttac gatcccgcag
     1201 tgggagatcc cgataacggc gtagctcccg gaaccgcgtg ggaggatgtt cccgaggact
     1261 gggtttgccc cgtatgcggt ttgggtaaag actcctttac ggaagaagat taatttggct
     1321 aaagtcggat tgcattttta gtgcagtccg acttttcttt ttatatgcag ttttgctaat
     1381 gtcgaaattt gttgattatt acgtcttgaa cacggttttg cgctgtgata aaatatcttc
     1441 agagatttgt aaacggagga
//
LOCUS       DBMDVO010000394         1454 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42030_length_1454_cov_1.434590, whole genome shotgun sequence.
ACCESSION   DBMDVO010000394 DBMDVO010000000
VERSION     DBMDVO010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42030_length_1454_cov_1.434590"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="ACZPE2_05910"
     CDS             complement(<1..236)
                     /locus_tag="ACZPE2_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR1319418.1"
                     /translation="MENIGYYNGKFDLIEKMTVPMNDRAMYFGDGVYDATYSVNRTIF
                     ALEDHLDRFYNSCRLLEIPAPMPREELKKTLYECV"
     gene            complement(417..1214)
                     /locus_tag="ACZPE2_05915"
     CDS             complement(417..1214)
                     /locus_tag="ACZPE2_05915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1319419.1"
                     /translation="MNNLLLNERSLIYHLRQYRSKRRLHYFAETESTNEVAKKLCGDG
                     KGLGAFVVSDCQTAGKGRSGRTFCSPKGTGVYISIVYEISGKEPNFDLLSSLAGLAVR
                     DTLFNFFDFDCKIKWPNDILLDGKKICGILCEIVNKNGKPKYAVVGIGLNIEKQEFPE
                     EIMYTAASIGDKYEGELDHNEIAVDIVNNLDRYIIRRDALNAENTGEIINRLKMYSAS
                     LGQIVRVITPDSEYDAKVLDIAPDGGLVILSDGETKTITSGEIVHIR"
BASE COUNT          376 a          306 c          303 g          469 t
ORIGIN      
        1 acgcactcat agagtgtctt tttaagctct tcacgcggca tcggagcagg tatttcgagc
       61 agcctgcagc tgttgtaaaa cctgtcgagg tgatcctcaa gcgcaaagat ggtgcggttt
      121 accgaatatg tagcgtcgta aactccgtcg ccgaagtaca tggcgcggtc gttcatcggg
      181 accgtcatct tttcaataag atcgaatttg ccgttgtaat aaccgatatt ttccattttt
      241 gtcagctccc caaaataatg ttataaaagc aaaagaagtc aagcccaatt gagttttgcc
      301 cgccatcgca cagaattcaa aaagacttaa cttacaataa gacattataa tacgtcattt
      361 tttacctgtc aataaaaatt aaacaaatcg taaaataatg tcgaaattga ctaaaattat
      421 cgtatatgta ctatttctcc cgaggttatg gtttttgttt caccgtccga cagaattaca
      481 aggccgccgt ccggggctat gtcgagaacc tttgcgtcat attcgctgtc gggcgttata
      541 accctgacga tttgccctaa agacgccgag tacattttca gcctgtttat tatttcaccg
      601 gtgttttcgg cgttgagggc gtcgcggcgt atgatgtagc ggtcaaggtt gtttactatg
      661 tcaaccgcta tttcgttgtg gtcaagctca ccctcgtatt tatcgcctat gcttgcggca
      721 gtatacatta tttcctccgg gaattcctgc ttttcgatat taagacctat tccgactacg
      781 gcgtatttcg gcttgccgtt tttatttact atttcacaca gtataccgca tatttttttg
      841 ccgtcgagta aaatatcgtt cggccatttt attttgcagt caaaatcaaa aaagttaaag
      901 agcgtatcgc gcacggcaag ccccgcaaga cttgacagca ggtcgaaatt gggctctttt
      961 ccgcttatct cgtaaacgat tgaaatgtat acgccggtac cctttggtga gcaaaatgtt
     1021 ctgccggagc ggcctttgcc ggcggtttga cagtcggata caacgaaagc tccgaggccc
     1081 ttgccgtcgc cgcaaagctt ttttgcgacc tcgtttgtgg attcggtttc ggcaaaataa
     1141 tgcagcctgc gctttgagcg atattgtctt aagtgatata taaggcttct ttcgttaagt
     1201 aaaagattgt tcatttatta tcagtccgtt tcggtttatt aatgatatat attgctgccg
     1261 cactcggtgc aatactgata ttttacataa tagtatttgt aaggtcaagt gtatttggaa
     1321 aaaaccgaca aatccgcgtt aaatgaatgt cgctaatccg tttgccgaaa aaatgtcgga
     1381 tttttgttat gaatttatat attatccatt gacttttttt gcgatttatt gtaaattata
     1441 ttatgttatg caac
//
LOCUS       DBMDVO010000395         1454 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42048_length_1454_cov_1.150037, whole genome shotgun sequence.
ACCESSION   DBMDVO010000395 DBMDVO010000000
VERSION     DBMDVO010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42048_length_1454_cov_1.150037"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(101..1441)
                     /locus_tag="ACZPE2_05920"
     CDS             complement(101..1441)
                     /locus_tag="ACZPE2_05920"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1319420.1"
                     /translation="MSIKLNAKYTEDFVSKTDLESIAPEIKKAHKTLTEKSGAGNDFL
                     GWVDLPENYDKEEFERIKKAAEKIRSDSQVLIVIGIGGSYLGARAAVEFCKSQNYNLV
                     CKDTPQIFFTGNSISSSALSDIVSLCENKDFSINVISKSGTTTEPAVAFRIFRELLES
                     KYGEEEAAKRIYVTTDKCKGTLKELSNKVGYETFVVPDDVGGRYSVLTAVGLLPIAVS
                     GCDIDKMMQGAADARKAYCDEDLDKNDCYKYAALRNILYRKGKSVEMMVSYEPDYTMM
                     NEWFKQLFGESEGKDHKGIFPASAVFSTDLHSMGQFIQDGTRLMFETAVVFDEPKHEV
                     TIKEEKSNSDGLNFLAGKTMSYVNRKAFEGTVLAHVDGGVPNIILELPKMDEYNFGYL
                     VYFLEKACAVSGYTLGVNPFNQPGVESYKKNMFALLGKPGYEDQKAELEARLGK"
BASE COUNT          382 a          344 c          269 g          459 t
ORIGIN      
        1 aaaagttttg tttttcctga gcccttgtgg ccaattataa gagataccat agaatctcct
       61 cctgtttatt aatatccgcc cttgcgggat ttaactgaat ttatttgccg aggcgagcct
      121 caagctctgc tttttgatct tcatatcccg gtttaccgag aagagcaaac atatttttct
      181 tgtagctttc tacacccggc tgattaaacg gattcacacc gagagtatac cctgatacgg
      241 cacaagcctt ttcaaggaag taaacaaggt aaccgaagtt atactcatcc attttgggaa
      301 gctccaaaat gatattcgga acgcctccgt caacgtgagc caacacggtt ccttcaaatg
      361 ctttacggtt tacatagctc atggttttgc ctgcaaggaa attaagtccg tcagaatttg
      421 atttttcctc tttaattgtt acttcgtgct ttggttcgtc aaatacaacc gcggtctcaa
      481 acataagtct ggtaccgtcc tgtataaatt ggcccattga atgaaggtcg gtggaaaata
      541 ccgcagacgc cgggaatatt cccttatggt ccttaccttc gctttctccg aagagctgct
      601 tgaaccactc gttcatcatt gtataatcag gctcgtatga gaccatcatt tcaaccgact
      661 tgccttttct gtaaagaatg ttgcgtaatg cagcatactt atagcagtcg tttttatcta
      721 aatcttcgtc acagtaagct tttctcgcat ccgctgcgcc ctgcatcatc ttatcaatgt
      781 cacagccgga aaccgctatc gggagaagac ccaccgctgt aagaaccgaa tatctgcctc
      841 cgacatcatc aggaacaaca aaggtctcat atccgacttt attcgaaagc tcctttaatg
      901 ttcctttaca tttatcggta gttacataaa ttcgctttgc agcctcttct tcgccgtact
      961 tgctctcaag cagctctctg aaaattctaa atgcaacggc cggctcggta gttgtacccg
     1021 atttggatat aacattaata gaaaagtcct tattctcaca aagagaaaca atatcggaaa
     1081 gagccgaaga gcttatactg tttccggtga agaatatctg cggcgtatct ttgcatacca
     1141 aattgtaatt ctgtgattta caaaattcaa cagccgctct tgcgccgaga tagcttcctc
     1201 ctatacctat aacaataaga acctgcgagt ccgaacgtat tttttcggct gcttttttta
     1261 ttctttcaaa ttcctcttta tcataattct ccggaagatc cacccatccg aggaaatcat
     1321 ttcccgcacc tgatttttca gtgagagtct tgtgagcttt tttgatttcg ggtgcgatgc
     1381 tctcaagatc ggttttagac acaaaatctt cagtgtattt tgcgttcagt ttaatagaca
     1441 ttgctgattg ttac
//
LOCUS       DBMDVO010000396         1449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42241_length_1449_cov_1.000000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000396 DBMDVO010000000
VERSION     DBMDVO010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42241_length_1449_cov_1.000000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            252..1085
                     /locus_tag="ACZPE2_05925"
     CDS             252..1085
                     /locus_tag="ACZPE2_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319421.1"
                     /translation="MVYSEKSKRYNGRKARIAIALVAAVCLSSSGCIAALYNYDVEIE
                     SAEAVSEITAQTSEAEAASTDGSETLDAVYQAKPDSLPRDDRAGRKEVFNEWVKAKYP
                     GPYEIYTPTEDEKWLFYDLKYWTAYDFCDVNSLTDEEMASSTVGYYGYADCSDQNFQE
                     YGFRYDELDGYYSKVGFETVKRLVESLYGRGYASSDYPHWGSEETEQNQYVCEAGADA
                     PEEDIFVESVYEVDEGVYYVIFGQEKLPDGTESDVTGMVIQRNSESAFGYNVIAARGY
                     C"
     gene            1221..>1449
                     /locus_tag="ACZPE2_05930"
     CDS             1221..>1449
                     /locus_tag="ACZPE2_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1319422.1"
                     /translation="MSYKEVYEELSLLGEGGMGKVYLVRHRRLGVLRAMKVVRKDDAT
                     KLPAEVDILKNLDHQRLPRLHDIYEDDQNIQI"
BASE COUNT          455 a          258 c          413 g          323 t
ORIGIN      
        1 aaaacgagat aattcaacaa agacggatta aataaaaaat aacacataaa catacgattg
       61 tcaaacaaaa cgacgtcgaa acacgcctgt ttgtaagagc aattatagag aaacacgcat
      121 tgataaaaat ctcaataccg aatttcatgt caattattac actaaattga ccggttatta
      181 catagatgag gcagcgctgc aagaatgcct taaaataaaa gggtaagctt cagagaggga
      241 aaatgtttaa tatggtatac agtgaaaaaa gcaaaaggta taacggccgg aaggcacgaa
      301 tagcaatagc gttggtggcg gcggtttgcc tgagcagcag cggatgcatt gccgctcttt
      361 ataattatga cgtcgaaatc gagagcgccg aagccgtttc ggagataacg gcgcagacgt
      421 ctgaggcgga ggctgcatcg acggacggga gcgagacgct ggacgccgtt tatcaggcta
      481 agccggattc gcttcctaga gacgacaggg cgggaagaaa agaagtattc aacgagtggg
      541 taaaagcaaa atatccggga ccgtatgaga tatacactcc gacagaggat gaaaaatggt
      601 tgttttacga cttgaaatat tggactgcat acgacttctg tgacgtgaac agtttgacag
      661 atgaagagat ggcgagttct actgttggat attatggata tgcggactgc tcagatcaga
      721 attttcaaga atacggattc cggtacgacg agcttgacgg gtattattca aaggtcggct
      781 ttgagaccgt aaagaggctg gtggaaagcc tgtacgggcg cggatatgca tcgagcgact
      841 atccgcattg gggcagcgag gaaacagagc agaatcaata tgtgtgcgag gcaggtgccg
      901 atgcgccgga ggaagacata tttgtggaga gcgtatacga agtggacgag ggagtttact
      961 atgtgatatt tggtcaggag aagctgccgg atggaacgga aagcgatgtt acgggaatgg
     1021 tgattcaaag gaacagcgag tcggctttcg gatataacgt tattgccgcc agaggatact
     1081 gttaagaata gctttatggc aaggaattcg acaattatta cattaaaagc ggcgattatg
     1141 cggttgggga aaaggcctgc gatttttgtg ctttaatcac atagagtgaa aaatcatctt
     1201 aatttttgcg aggtcgtcag atgtcttata aggaagtata tgaagaactg tcattgcttg
     1261 gcgaaggggg aatgggtaag gtatacctcg tccggcaccg gcggctgggc gtgctgaggg
     1321 cgatgaaggt ggtccgaaag gacgatgcaa ccaaattgcc cgccgaggtg gatatactga
     1381 aaaaccttga tcaccagcgg ctgccgcggc tgcacgacat atatgaggac gaccaaaata
     1441 tacagatca
//
LOCUS       DBMDVO010000397         1448 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42280_length_1448_cov_1.297699, whole genome shotgun sequence.
ACCESSION   DBMDVO010000397 DBMDVO010000000
VERSION     DBMDVO010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42280_length_1448_cov_1.297699"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(60..422)
                     /gene="ylxM"
                     /locus_tag="ACZPE2_05935"
     CDS             complement(60..422)
                     /gene="ylxM"
                     /locus_tag="ACZPE2_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1319423.1"
                     /translation="MANNVEITILLDFYGDMLTAKQRDFINLYYNDDLSLAEIAENEG
                     ITRQGVRDAIKRAEGQLYEMESRLGFVKKWNGIKTTLGDIINCAEEISDYNLNHGLSR
                     EINDTTVKIKSLALSIIE"
     gene            complement(516..1046)
                     /locus_tag="ACZPE2_05940"
     CDS             complement(516..1046)
                     /locus_tag="ACZPE2_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR1319424.1"
                     /translation="MKKLFSILSAAVIVLSVAAVMAFAVGDGKNRGDVNGDGKVDSAD
                     AMLVMKCDAGLLSLSNDEIKRADLNGDGKIDSCDAVYILRIAAGLKVPDAETTAKETS
                     SVSEPTTEETTKPTTENTTKPTEPVTEPTRPATKPSEPVTKPTEPTTTPTTADSNWDP
                     SKNAVIDWGGGTTKKN"
     gene            complement(1061..>1448)
                     /locus_tag="ACZPE2_05945"
     CDS             complement(1061..>1448)
                     /locus_tag="ACZPE2_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319425.1"
                     /translation="AVALLILMISVVFISCGKKRSWEKDDTTAAPSETTSIEYTTDES
                     GEKFVTNINGELIPVTTGKDGSVEFYEDLITKTSEQAAETEPNGKDEPQKPNEAPTKG
                     ESGTVKIGTGDPADESHAAVIDWSAR"
BASE COUNT          334 a          351 c          322 g          441 t
ORIGIN      
        1 taaaagcggc ttcaagtctg tccgataatc cttcaaatgc cataaactta cctctgtaat
       61 tactctatga tagacaaagc aagagatttt atctttaccg tagtgtcgtt tatctctcga
      121 gaaagcccgt gatttaaatt ataatcgctg atttcctcgg cacagtttat aatatcgcca
      181 agtgtggttt ttatgccatt ccattttttt acgaatccga gtctcgactc catctcgtag
      241 agctgcccct cggcacgttt tattgcatcg cgcacaccct gacgggttat gccctcgttc
      301 tcggcaattt cggcaagaga aaggtcgtcg ttataataaa gattgataaa atcacgctgt
      361 ttcgcagtaa gcatatcacc gtagaaatca agcaaaatcg ttatttcaac attatttgcc
      421 aaaactacca ctccttaaag aaaaagtccc tgtaaagcca gcgactttac aaggacatat
      481 tatactaaat tatattgtaa ttgtcaagaa aaagtttagt tcttcttggt tgttccgccg
      541 ccccagtcaa taacggcgtt ctttgagggg tcccagttgg agtcggcggt tgtgggcgtt
      601 gtggtgggtt cggtgggctt ggttacgggc tcagagggtt ttgtagccgg ccgggtgggc
      661 tcggttacag gctccgtggg cttcgtagta ttttccgttg tcggcttcgt ggtttcttcg
      721 gttgtcggct cggaaacgga cgaagtctct tttgccgtag tctcggcatc gggaacctta
      781 agccctgccg ctatacgcaa aatatatacg gcgtcgcacg aatcaatttt accgtcaccg
      841 tttaaatcgg ctcttttgat ttcgtcattt gagaggctca aaagtccggc atcacatttc
      901 ataacaagca tggcatctgc cgaatcaact ttaccgtcgc cgtttacgtc tccgcggttt
      961 ttaccgtcgc cgacagcaaa agccattacc gccgcaaccg acagcacaat tacggccgct
     1021 gacagaatac tgaatagttt tttcatttta gttcctttta ttatcgagca ctccaatcaa
     1081 taacagccgc atggctctca tccgcaggat cacctgttcc tatttttacg gtaccgcttt
     1141 cgcctttagt gggagcttca ttcggctttt ggggctcatc cttgccgttc ggctcggtct
     1201 cggcagcctg ctcggaggtc ttggttataa ggtcttcgta gaactcaacc gaaccgtcct
     1261 tgccagtggt tacgggaata agctctccgt ttatgtttgt tacgaatttt tcgcccgatt
     1321 catcggttgt gtattcaatg gacgttgttt cggagggagc ggctgtagta tcgtcctttt
     1381 cccagcttct cttttttccg caggaaataa acaccaccga aatcataagt atcaaaagtg
     1441 cgacagca
//
LOCUS       DBMDVO010000398         1443 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42485_length_1443_cov_1.232489, whole genome shotgun sequence.
ACCESSION   DBMDVO010000398 DBMDVO010000000
VERSION     DBMDVO010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42485_length_1443_cov_1.232489"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="glnA"
                     /locus_tag="ACZPE2_05950"
     CDS             <1..1256
                     /gene="glnA"
                     /locus_tag="ACZPE2_05950"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR1319426.1"
                     /translation="FTDIFGQLKNVAITASQLEKALDNECMFDGSSIEGFVRINESDM
                     YLRPDYDSFVILPWKDRVARLICDVYCADGNTPFLGDPRNVLKKVVKEAADMGYTFNV
                     GPECEFFLFQLDEDGNPTTKTGDVAGYFDMGPADQGEQCRRDICLALESMGFEIEASH
                     HEVAEGQHEIDFRFDEVVKTADNVMTFKHVVKTYARRHGLHATFMPKPVFGINGSGMH
                     TNMSLTKDGKNAFYDENSEFGLSETALHFIAGVLKHVKGITAIANPLVNSYKRLVPGY
                     EAPVYIAWSASNRSSLIRVPAARGKGTRIELRSPDPSCNPYLEMALCLAAGLDGIKNN
                     LTPPQNTTKNIFEMSAEELAADGIDYLPGTLEDAMNEFQADPFMKETLGDHIYEKYVE
                     GKLREWDEYRTSVSNWETNRYLTKY"
BASE COUNT          383 a          336 c          333 g          391 t
ORIGIN      
        1 agtttactga tattttcggt cagcttaaaa atgtcgcaat taccgcgagt cagcttgaaa
       61 aggctcttga caacgagtgt atgttcgatg gctcctcaat agagggcttc gtaagaatca
      121 atgagtcaga tatgtatttg agacccgatt acgattcgtt cgttatactt ccttggaagg
      181 acagagttgc acgccttatc tgcgacgttt attgcgctga cggcaatact ccgttcctcg
      241 gcgatccgag aaacgttctt aaaaaggtag ttaaagaggc tgccgatatg ggctatacgt
      301 tcaatgtagg ccccgagtgt gaattcttcc tcttccagct tgatgaggac ggaaatccca
      361 ccacaaaaac aggcgatgtt gcgggttact ttgatatggg ccccgccgat cagggtgagc
      421 agtgcagaag agacatttgc cttgcgctcg agagcatggg ctttgaaatc gaggcttcac
      481 accacgaagt tgccgagggc cagcacgaga tagacttccg ttttgatgag gttgttaaga
      541 ccgcagacaa cgttatgacc tttaaacacg tagtaaagac ctatgcgcgc cgtcacggcc
      601 ttcatgcaac atttatgccg aagcccgtat tcggtataaa cggctccggt atgcacacca
      661 atatgtcgct tacaaaggac ggtaaaaacg ctttctatga tgaaaattcc gagtttggac
      721 tttccgagac cgctcttcac ttcattgctg gtgtattaaa gcacgtaaag ggtattaccg
      781 ccatagcaaa tccgcttgtt aactcttata agagacttgt tccgggatat gaagctcccg
      841 tatatatagc ttggtcggct tcaaaccgtt cctcgcttat ccgtgttccc gcagctcgcg
      901 gaaagggcac cagaatagag ctccgttctc ccgacccgtc atgcaacccc tatcttgaaa
      961 tggctctttg ccttgcagca ggtcttgacg gtatcaaaaa caaccttact cctccgcaga
     1021 acaccacaaa gaacatcttt gaaatgtccg cagaagagct tgccgcagac ggcatagatt
     1081 atctccccgg tactcttgag gacgccatga atgaattcca ggctgatccg tttatgaaag
     1141 agacgctcgg cgaccacatc tatgagaagt atgttgaggg taagcttcgc gagtgggatg
     1201 agtatcgtac cagcgtaagc aactgggaaa caaacagata tttaactaaa tattgataat
     1261 acctcatttt ttctcctgcg aaaagggaac ggcgattttg ccgttccctt ttccattttt
     1321 ttatgaggct ttgaatttaa tttgccttat tggcgcataa aaaaacacgg ctgacaaaag
     1381 gccgaatatt cagccaaagt caaccgtgct tttgtaattt ttattcggtt tcttttttga
     1441 agc
//
LOCUS       DBMDVO010000399         1442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42522_length_1442_cov_0.893363, whole genome shotgun sequence.
ACCESSION   DBMDVO010000399 DBMDVO010000000
VERSION     DBMDVO010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42522_length_1442_cov_0.893363"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="ACZPE2_05955"
     CDS             complement(<1..121)
                     /locus_tag="ACZPE2_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1319427.1"
                     /translation="MVNFEIEKEYISDGFNCICGVDEAGRGPLAGPVCAAAVIL"
     gene            complement(121..987)
                     /gene="ylqF"
                     /locus_tag="ACZPE2_05960"
     CDS             complement(121..987)
                     /gene="ylqF"
                     /locus_tag="ACZPE2_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1319428.1"
                     /translation="MNDTIKQNVQWFPGHMAKTRRKIKESLPLVDAVVEILDARIPRS
                     SQNPEIAEIIGEKPHIAILNKCDIADDAATKKWIEYFRQNGIYALALDCKTGKGLNRF
                     VPLVNEVLKDKIQSNIDKGMAGRQIRLMVVGIPNTGKSSFINKMAGKKRAEVADKAGV
                     TRHNQWFAIGGGLELLDTPGVLWPKFEDPEVGDKLAFVGSVKDQVVDIETLAVRFLRV
                     MRDFYPERLTERYKIENFEELEPYEILELIGKKRGMIMRGGVVDTERASVMLLDEYRG
                     GKLGKITLDGIY"
     gene            complement(980..>1442)
                     /gene="lepB"
                     /locus_tag="ACZPE2_05965"
     CDS             complement(980..>1442)
                     /gene="lepB"
                     /locus_tag="ACZPE2_05965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1319429.1"
                     /translation="TFLFKISSVNGPSMLDSFHNGDRVFITAAGYQPKYGDVVIISQP
                     NQYNEVLIKRIIAIGGQTVDVDATAGTVTVDGKVLYEPYIREQTLTQGNMNFPLTVPE
                     GYVFVMGDNRNHSTDSRYKSIGLIDERYIMGKAFYRVGDKTLLPSYGVSYE"
BASE COUNT          399 a          366 c          248 g          429 t
ORIGIN      
        1 gcagtataac cgcagcagca catacaggtc ccgcaagcgg tccgcgtcct gcttcgtcca
       61 ctccgcaaat acagttaaag ccatcggaaa tgtattcttt ttcaatttca aaattcacca
      121 tcaataaatt ccgtccaaag taatttttcc gagcttaccc ccacggtact cgtcgagcag
      181 cattacggaa gctctctcgg tatcgacgac gccgccgcgc ataatcatac cgcgcttttt
      241 gcctataagc tcaagtattt catacggttc aagctcttca aagttttcaa ttttatatct
      301 ttcggtgagc ctttcgggat aaaaatcgcg cataacgcga agaaaacgaa cagcaagcgt
      361 ttcaatatca acaacttgat ctttaaccga cccgacaaaa gctaatttat cgccgacttc
      421 cggatcttca aatttaggcc acaaaacgcc cggagtatcc agaagctcaa gccctccgcc
      481 tattgcgaac cattggttat gccttgtaac tcccgcttta tccgcaacct cggctcgttt
      541 tttgccggcc attttgttta taaatgacga tttacctgta ttgggaatac cgactaccat
      601 aagacgtatt tgtctaccgg ccatgccttt atctatatta ctctgtattt tatctttgag
      661 gacttcattt acaagcggaa caaatcgatt taagccctta ccggttttac aatcaagtgc
      721 caaagcatat ataccgtttt gccgaaaata ttcaatccat ttttttgtag ccgcatcatc
      781 tgcgatatca cacttattaa gtattgctat atgcggcttt tctccgatta tttcagcaat
      841 ttccgggttt tggctgcttc gcggaatgcg agcatcaagt atttcaacaa ctgcatcaac
      901 aagcggcagg ctttccttta tctttcggcg cgttttcgcc atatgcccgg gaaaccattg
      961 tacattttgt tttatagtat cattcataac taacaccata gctcggcaaa agcgttttat
     1021 cccctaccct gtaaaaagcc ttgcccatta tatatctttc atcaataagt ccgattgatt
     1081 tatagcgact gtccgtagaa tgattacggt tatcacccat tacaaaaaca tatccctcag
     1141 gaaccgtcag cggaaaattc atattgccct gcgttaaagt ttgctctctt atatacggtt
     1201 catataaaac tttgccgtca accgtaacag tacccgcagt cgcgtccaca tcaacagttt
     1261 gaccgccaat cgctatgatt cttttaataa gcacctcatt atattgattg ggctgagata
     1321 ttataacaac atcaccgtac ttcggctgat atccggcagc agtaataaag actctgtctc
     1381 cgttgtgaaa cgaatccaac atcgacgggc cgttaaccga agatatttta aacagaaaag
     1441 ta
//
LOCUS       DBMDVO010000400         1440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42597_length_1440_cov_1.199403, whole genome shotgun sequence.
ACCESSION   DBMDVO010000400 DBMDVO010000000
VERSION     DBMDVO010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42597_length_1440_cov_1.199403"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="ACZPE2_05970"
     CDS             <1..451
                     /locus_tag="ACZPE2_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014657.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1319430.1"
                     /translation="NVGSSKKFSYSFVPDGIDFTDNVSVEGEVVNNTDIVSVKAVAKY
                     VLKTNCDRCGAPVERKTEVPIEHILISHLNDEDNDEFILVEDMKLDLDSLVREDMILA
                     LPAKILCRDDCKGLCQYCGKNLNLGSCSCKKPIDPRLQALADLLIDE"
     gene            483..671
                     /gene="rpmF"
                     /locus_tag="ACZPE2_05975"
     CDS             483..671
                     /gene="rpmF"
                     /locus_tag="ACZPE2_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1319431.1"
                     /translation="MAVPKRRVSSARRDKRRSNVWKMEAPELQKCPNCGEYKRAHRVC
                     PACGMYNGRQVLSKKEEA"
     gene            762..>1440
                     /locus_tag="ACZPE2_05980"
     CDS             762..>1440
                     /locus_tag="ACZPE2_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319432.1"
                     /translation="MSVKISSVKPFSKCWFHGIKSGDELLSVDGKEVTDILDYDFYLS
                     FPPVVLNFRTASGKLIDIPFKNDDTGLKFRTYLMDQQHHCKNGCIFCFIDQLPKGMRE
                     SLYFKDDDSRLSFLFGNYVTLTNITEHEVDRIIRMHISPINISVHTMNPELRVKMMKN
                     KNAGKCLSIIKRLSDAGIKMNAQLVLCPGINDGDELRYSIEELSKYMPAVQSIACVPV
                     GLTKYRDG"
BASE COUNT          421 a          261 c          341 g          417 t
ORIGIN      
        1 taatgtaggc agctcaaaaa agttttctta cagctttgtt cccgacggaa ttgattttac
       61 cgataatgtt tcggtagaag gcgaagtcgt taacaatacg gatattgtct ccgttaaagc
      121 tgttgcaaag tatgttctga aaacaaattg cgacagatgc ggtgctccgg ttgaaaggaa
      181 aactgaggtc ccgattgagc acatccttat ttctcatctt aatgatgaag ataatgatga
      241 gtttattttg gtcgaggata tgaagcttga ccttgattcg cttgttcgag aagatatgat
      301 tttagccttg ccggctaaga ttttgtgccg tgacgattgc aagggcttgt gtcagtattg
      361 cggtaaaaat cttaatctcg gcagctgttc gtgcaaaaag ccgattgacc caaggcttca
      421 ggcactggca gatctgctga tagatgaata aattatttat tttaacataa ggagagctaa
      481 aaatggcagt accgaagaga agagtttcat cagcaagaag agataagaga cgttcaaacg
      541 tttggaaaat ggaggctccc gagcttcaga agtgccctaa ctgcggcgaa tataagagag
      601 ctcacagagt ttgcccggca tgtggtatgt ataacggcag acaggtcctc tctaaaaagg
      661 aagaagcata atttatctta tacctttgtt tgagtgttaa aaggtggaga aggttttctt
      721 ccccgccttt ttgtcgtctt taaaaatggg aggtgacttt gttgtcggtt aaaataagca
      781 gtgttaagcc gttttcaaaa tgctggttcc acggtattaa aagcggcgac gagcttttgt
      841 ccgtagacgg caaggaagtt accgatatcc ttgactacga tttctatttg agtttcccgc
      901 ccgtggtttt gaatttcaga accgcctccg gtaagcttat agatattccg tttaaaaacg
      961 acgatacggg tcttaagttc cgcacttatc ttatggacca gcagcatcac tgtaaaaacg
     1021 gctgtatctt ttgctttata gaccagctgc cgaaaggaat gcgcgaaagt ctttatttta
     1081 aggatgatga cagcagatta tcgtttttgt ttggcaatta cgtaacactt acaaacatta
     1141 ctgagcatga ggttgaccgt atcatacgta tgcatataag cccgataaac atttcggttc
     1201 acaccatgaa tccggagctt cgcgtaaaga tgatgaaaaa taaaaacgcg ggtaagtgtt
     1261 tatcgattat taaacgattg tccgatgcgg gaataaaaat gaatgctcag cttgttttgt
     1321 gtccgggtat aaacgacgga gacgaacttc gttacagtat tgaagaattg tcaaaatata
     1381 tgcctgcggt tcaaagcata gcctgtgtgc cggtaggcct tacaaaatac agagacgggc
//
LOCUS       DBMDVO010000401         1439 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42645_length_1439_cov_1.450673, whole genome shotgun sequence.
ACCESSION   DBMDVO010000401 DBMDVO010000000
VERSION     DBMDVO010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42645_length_1439_cov_1.450673"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..>1439)
                     /locus_tag="ACZPE2_05985"
     CDS             complement(1..>1439)
                     /locus_tag="ACZPE2_05985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319433.1"
                     /translation="RWARLPYYMWTTLQFIRGVLTTIISFIIIIPLLKVGFVKTGDTF
                     FERPLFIITIVASIAIMAVVILIVASNINKSYLEANEKYAELDRIFYFFIDILGDYKT
                     GKEIRLYKEQGLVDSIATQKILTDGELTLRRISMKTAKSSSFIAILGATVGFGVYLFI
                     GVKGLFGLFGISSLVLYCGSFMQIISGIMMLANTLGKLKEILPFAADYFKILEAESTM
                     VYGNRELDTSDGFEIEFNNVSFKYPKSDNYALKHINLKIGDGKKLAVVGRNGSGKTTF
                     IKLLCRLYDIDDGEILINGINIKEYTRESLSSLFSVVFQDYKLLSLTVADNISASSKC
                     DESSLYSALEKADIKNRIEEMPQKERTYLYKDLDKSGVEISGGEAQKLALARALYKDS
                     PIVILDEPTAALDPIAENEIYNHFNSFVENKTAIYISHRLSSCAFCDEIAVFDNAELV
                     ETGTHNELVSANGKYAELWNAQAGYYLE"
BASE COUNT          415 a          319 c          268 g          437 t
ORIGIN      
        1 ttattctaaa taatagcctg cctgtgcatt ccagagctct gcgtatttac cgttagccga
       61 cacaagctcg ttatgcgttc ctgtctcaac cagttcggcg ttatcaaata cagcgatttc
      121 atcacagaag gcacagcttg aaagccgatg tgaaatatat attgctgttt tgttttcaac
      181 aaatgaatta aaatgattgt atatttcatt ttcagctatc gggtcaagcg cagccgtcgg
      241 ctcatcaagg attactatcg gcgaatcctt atataatgct ctggcaagtg ccagcttttg
      301 tgcctcacca ccggagattt ccacacctga cttatctaaa tccttatata gataagtcct
      361 ttctttttgc ggcatttcct caattctatt cttaatatcg gctttttcaa gagcagaata
      421 aagtgagctt tcatcacatt tactacttgc cgaaatgtta tctgcaactg tcaatgataa
      481 gagtttataa tcctgaaaca caactgaaaa aaggctgctc agactttctc ttgtgtattc
      541 tttgatattg attccgttaa ttaaaatttc gccatcgtca atatcataca aacggcaaag
      601 cagcttaata aaagtcgttt tgccactgcc gtttctgccg actacggcaa gctttttgcc
      661 gtcaccgatt ttcaggttaa tgtgcttaag ggcataatta tcgctctttg gatatttaaa
      721 cgaaacatta ttgaattcaa tctcaaatcc gtcagatgta tcaagttcgc ggttgccgta
      781 aaccattgta ctctcggctt cgagtatttt gaaataatcc gcggcgaacg gcaaaatttc
      841 tttcagctta ccgagtgtgt tggcaagcat catgataccg ctgatgattt gcataaatga
      901 gccgcagtac aatacaagcg agctgatacc gaacaaaccg aataggcctt tgacgccgat
      961 aaacaaatat acgccgaacc cgaccgttgc gccgagtatg gcaataaatg agctcgattt
     1021 tgctgttttc attgaaatcc ttcgaagcgt taattcaccg tctgtcagta tcttttgcgt
     1081 tgcaatactg tcaaccaagc cctgttcctt atacagccga atctccttgc ccgttttgta
     1141 atcacctaaa atgtcgataa agaaatagaa aatgcggtcg agctcggcgt acttttcgtt
     1201 tgcttcaaga taagatttat tgatatttga cgcaacaatc aatattacca ccgccataat
     1261 tgcaattgat gcaactatag taatgataaa cagtggcctt tcaaagaaag tatcgcccgt
     1321 tttgacaaat ccgaccttca gaagaggaat gatgataata aacgagataa tcgtggtcag
     1381 cacgcctcgt ataaattgaa gcgttgtcca catatagtac ggaagtctcg cccaacggc
//
LOCUS       DBMDVO010000402         1436 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42761_length_1436_cov_0.877903, whole genome shotgun sequence.
ACCESSION   DBMDVO010000402 DBMDVO010000000
VERSION     DBMDVO010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42761_length_1436_cov_0.877903"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="ACZPE2_05990"
     CDS             complement(<1..645)
                     /locus_tag="ACZPE2_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319434.1"
                     /translation="MKKLIAVFICLVMIAGIAIATVSADAKILFYDPFDTKKENDWLW
                     DSSNFYVKDGVLMGDPTAVIHQSAYDPKYGQIRRWDTFTNKVDIKIVDYDESNFIGSG
                     LWWRDYNTSFEEENSGANEDGEIWTYRYNYVTNSVILESDYLTNKGMTAKTYQLPRNA
                     VKIGPDDDPTTFSLGWRIQPGKIECYFNNQLVITCTDVPADLGTHRESPILLMNH"
     gene            complement(756..>937)
                     /locus_tag="ACZPE2_05995"
     CDS             complement(756..>937)
                     /locus_tag="ACZPE2_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319435.1"
                     /translation="ETNDKGETIVVTKVVADTNANNPSANNGGSNTGDMIVAAAAVMA
                     VAAAVAVIYAKRREH"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<948..>1436)
                     /locus_tag="ACZPE2_06000"
     CDS             complement(<948..>1436)
                     /locus_tag="ACZPE2_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319436.1"
                     /translation="DYDDSTLVGPGLWFRDYNTTYDQQDEGEEDTGEVWWYEYDYISN
                     SVILRGDHFENNKLEPVKVKLEDGIVKAGPDEKPTTFSIGWRIQPGHIECYFNDKKVI
                     DYTNVPMTIGSVRPSPIVLWNNSCYIEFDNFIVSTPDYNLFNEVPGDNGGNNGGNQQP
                     GNA"
BASE COUNT          325 a          330 c          337 g          434 t
ORIGIN      
        1 atggttcata agaaggatcg gggattctct gtgagttccg aggtcagcgg gaacatcggt
       61 gcaggtaata acaagctggt tattgaagta gcactcgatc ttgccgggct gaattctcca
      121 gccgagggag aacgtagtcg gatcatcatc gggaccgatc tttacggcat tgcggggaag
      181 ctgatatgtc ttcgcagtca ttcccttgtt cgtaagatag tcgctttcga gaattacgga
      241 gttggtcaca taattgtatc tgtaggtcca aatctcgccg tcttcgtttg ctccggaatt
      301 ttcctcttca aaagaggtgt tgtagtctct ccaccagagt ccgctgccga taaagttcga
      361 ctcatcgtaa tcaacgattt tgatatcgac tttgttggta aaggtatccc aacggcggat
      421 ctgaccgtat ttggggtcgt atgcgctctg atggataact gcggtgggat cgcccataag
      481 aactccgtct ttcacataga aattcgaaga atcccacagc cagtcatttt ccttttttgt
      541 gtcaaacgga tcgtagaaca ggatttttgc atccgcgctg acggtcgcaa ttgcaattcc
      601 ggcgatcatc accaggcaga tgaaaacagc aataagtttt ttcatgctca tttttccttt
      661 gcttttattt tattaaggta tgattcgtga ttatatttta tcataagagt acgcgaaaat
      721 caatactgtt tttttaattt tcgcagatta atttgtcagt gctcccgccg ttttgcatat
      781 ataaccgcaa ctgccgccgc aactgccatt accgccgcag ccgcaacgat catatctcct
      841 gtgttggagc cgccgttgtt tgcgctcgga ttgtttgcat tggtatcggc aacgaccttg
      901 gtgacgacaa tggtttcgcc tttgtcgttc gtttcatnnn nnnnnnngcg ttgccgggct
      961 gctgatttcc gccgttgttg ccgccgttat cgccggggac ctcattgaag aggttataat
     1021 cgggagtgct tacgataaag ttgtcgaact caatgtagca gctgttgttc cagagaacga
     1081 taggagacgg tctgacagag ccgatcgtca tcggaacatt tgtataatcg atgacctttt
     1141 tgtcattgaa atagcactcg atatgtccgg gctgaattct ccagccgatc gaaaaagtgg
     1201 tgggcttctc gtcgggtccc gcttttacga ttccatcctc gagctttacc ttgacaggct
     1261 caagcttgtt gttttcaaaa tggtcgcctc tgagaattac cgagttgctg atatagtcat
     1321 attcatacca ccatacttca ccggtatctt cttcgccctc atcctgctga tcgtatgtag
     1381 tgttgtaatc tctgaaccaa agtccgggac cgaccagcgt tgaatcatca taatcg
//
LOCUS       DBMDVO010000403         1435 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42782_length_1435_cov_1.332084, whole genome shotgun sequence.
ACCESSION   DBMDVO010000403 DBMDVO010000000
VERSION     DBMDVO010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42782_length_1435_cov_1.332084"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="ACZPE2_06005"
     CDS             complement(<1..760)
                     /locus_tag="ACZPE2_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319437.1"
                     /translation="MLSVVSGAVLLGFQYALLALGVYISFRILNIPDLTVDGSFTFGI
                     AVSAVLCASGHPFLGLTAAVFAGFAAGIVTGFLQTKAKIHPILAGILTMSGLYTVNIT
                     VMGAPNVSLIGKKRFYDGFASLFGENSSGDLPKMILAAVICAICVALLIMFFKTVLGL
                     CIRATGNNKDMVRASSINTDITTVTALAISNALVALSGAVIAQTQGFADVNSGSGMIV
                     IGLASVIIGEVIFGKRSLSVGLVSAVVGSVIYRMI"
     gene            complement(889..>1435)
                     /locus_tag="ACZPE2_06010"
     CDS             complement(889..>1435)
                     /locus_tag="ACZPE2_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1319438.1"
                     /translation="SNIVPVDQIFGLAKELTPDVKKIGILYCSGEDNAVKTAAKAKDY
                     LSQSGYAYEEVTVTNSSEVQSAATSLAAKCDALYIPIDSTVQAAMEQVVEAATAKKIP
                     VYGSDPVMVQKGALASVSVSNTQLGEKAADMADKILKGTPVSEVPAVALTDYQYVINS
                     KTASTLGIKTPTGGNYQFIGA"
BASE COUNT          363 a          344 c          361 g          367 t
ORIGIN      
        1 ctatcattct gtaaattacg gaaccgacga ctgccgaaac aagccctact gaaagcgagc
       61 gtttgccgaa tatgacttcg cctattatta ccgaggcgag acctataact atcataccgc
      121 tgccggagtt tacgtcggca aagccctgtg tttgagctat cacggcaccc gaaagtgcaa
      181 cgagcgcgtt tgatattgca agtgcggtta cggtggttat gtcggtattt atcgaggagg
      241 ctcttaccat atccttgttg tttcccgtgg ctcttataca taagccgaga acggttttaa
      301 agaacataat cagcagtgct acgcatatcg cgcagattac cgccgccaaa atcattttag
      361 gcagatcccc cgagctgttt tcgccgaaca gtgacgcgaa accgtcataa aatctctttt
      421 tgcctatcaa agatacattg ggagcaccca taacagttat attgactgtg taaagaccgc
      481 tcatggtgag tattccggca agtatcgggt gaatttttgc ctttgtttgt aaaaatcccg
      541 tgacaattcc tgcggcaaag ccggcgaata cggccgccgt aagacccaaa aacggatgtc
      601 ccgaagcgca caggactgcg gagacggcaa tgccgaaggt gaagcttccg tcaacggtga
      661 ggtcgggaat gttcagtatg cggaatgaaa tatagacgcc gagtgccaaa agagcgtatt
      721 gaaagccgag cagcaccgcg cccgaaacaa cagaaagcat aagctttcct cctaacaaaa
      781 atttaaaggg gctgcaaaat actcggaaag cgacaatcaa agcggcgtgt taaagctctg
      841 ccgccgagat gcttttcgaa aattttccta acccctgaaa gactcaaatt atgcgccgat
      901 gaactgatag ttgccaccgg taggtgtttt aataccgagt gtgcttgcgg tcttgctgtt
      961 tatgacatac tgataatccg tgagcgcaac tgcgggaacc tccgatacgg gagtaccttt
     1021 taagatttta tcagccatat cggcagcttt ttcgccgagc tgtgtgttgc ttacgctgac
     1081 gcttgcaagc gcgcctttct gaaccattac ggggtcgctg ccgtatacgg gaatcttttt
     1141 ggctgttgcc gcttctacta cctgctccat ggcagcctgt acggtggaat ctatggggat
     1201 gtaaagagca tcacactttg cggcgagcga ggttgcggcc gactgtacct ctgaggaatt
     1261 tgtaacggtt acctcttcgt aggcatagcc gctctgtgag aggtaatcct tagccttagc
     1321 ggcggttttt acggcgttat cttcgcccga gcagtagagt atgcctattt ttttgacatc
     1381 gggagtaagc tcctttgcaa ggccgaatat ctggtcaacc ggaactatgt ttgag
//
LOCUS       DBMDVO010000404         1435 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42806_length_1435_cov_0.651424, whole genome shotgun sequence.
ACCESSION   DBMDVO010000404 DBMDVO010000000
VERSION     DBMDVO010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42806_length_1435_cov_0.651424"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1435
                     /locus_tag="ACZPE2_06015"
     CDS             <1..>1435
                     /locus_tag="ACZPE2_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1319439.1"
                     /translation="TGAGKSIVIDSINAVLGERTSRELIRAGCDTAEVSAVFGELDNA
                     TAAALSDIGVVPDDDGNIVIRRRLSAAGKGLIKLNDRPVTATELKEVGRHLVNIHGQH
                     DNQALLDPERHLDYVDAVADDDPIKGKYYAEFREINKIRKELAAVQTDEDEKRRRVDL
                     LKYSINELESAGIKPGEYDKLRRKLEIARNYQKTAEALARAYSALKGDDELDGAVSLL
                     SGAEKALSSLRDEEWDKRASVLSSAASAAEDVSAAIYDFLENAEYSDIDPDEINARLD
                     TLDKLMLKYGGGEWELLKALDKAREELNDIEFSDKKAAELSELLDSSVERLVALGEEL
                     TEVRKKAAAAFEKQVCDILSYLNMPDVRFTVQFAKGRYTKRGCDTAEFMISANRGESI
                     KPLSKIASGGELSRVMLAIKSVLLGRDPVGTMIFDEIDTGISGYTAGKVGTQLKRVAE
                     NRQVICVTHLAQIAAMGDTHLLIEKKA"
BASE COUNT          375 a          313 c          441 g          306 t
ORIGIN      
        1 aaacgggcgc gggaaaatcc atagtgattg attcgataaa cgcagtgctc ggcgagcgca
       61 cttcgaggga gcttatccgc gcggggtgcg atactgcaga ggtgtccgcg gttttcggcg
      121 agcttgataa cgcgaccgcc gcggcgctgt cggatatcgg cgttgtgccg gatgacgacg
      181 gcaatattgt aataaggcgt cggctttccg cagcgggtaa ggggcttata aagctcaatg
      241 accgccccgt aacggcgacc gagcttaagg aggtcggcag gcatcttgta aatattcacg
      301 gtcagcatga taatcaggcg cttttagacc cggaacggca tttggattat gttgacgcgg
      361 tggcggacga cgaccccatt aaaggtaaat attatgcgga gttccgtgag ataaacaaaa
      421 tcagaaaaga gcttgccgct gttcaaaccg atgaggatga aaagcgccgc agggtcgatt
      481 tgcttaaata cagcataaac gagcttgaat cggcgggaat aaagcccggc gagtatgaca
      541 agcttcgcag gaagcttgaa atcgcaagga attaccaaaa aaccgcagaa gcgcttgccc
      601 gcgcgtattc cgcacttaag ggtgacgatg agcttgacgg cgcggtatca ttgttaagcg
      661 gcgcggaaaa agccctttcg tccctgcggg atgaggaatg ggataagagg gcttccgtac
      721 tgtcgtctgc ggcatcggct gcggaggatg tgagcgcggc tatttacgat tttcttgaaa
      781 atgcggagta ttcggatata gatcccgatg aaataaacgc gcggcttgat acgctcgata
      841 agcttatgct taaatacgga ggcggagagt gggagctgct caaagcgctt gataaggctc
      901 gcgaggagct aaatgacatc gagttttcgg ataaaaaggc ggcagagctg tcagagttgc
      961 ttgacagctc cgtggagagg cttgtggctc tcggcgaaga gcttaccgag gtaagaaaaa
     1021 aagccgcggc ggcgtttgaa aagcaggtct gcgatattct ttcttatctt aatatgccgg
     1081 atgtccgctt taccgtgcag tttgcaaagg gcagatacac caagcgcggg tgcgatactg
     1141 cggaatttat gatttccgca aaccgcggcg agagcataaa gccgctttca aaaatcgcct
     1201 cgggcggtga attatcccgc gtgatgctgg caattaaaag cgtgctgcta ggccgtgacc
     1261 ccgtgggcac tatgattttt gatgaaattg atacgggaat cagcggttac accgcaggaa
     1321 aggtcggaac gcagctcaaa agggttgcgg aaaacaggca ggtcatatgc gtaacgcatt
     1381 tggcgcagat agcggcaatg ggggatacgc acctgcttat tgaaaagaag gcgga
//
LOCUS       DBMDVO010000405         1433 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_42884_length_1433_cov_1.052553, whole genome shotgun sequence.
ACCESSION   DBMDVO010000405 DBMDVO010000000
VERSION     DBMDVO010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42884_length_1433_cov_1.052553"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="ACZPE2_06020"
     CDS             complement(<1..1295)
                     /locus_tag="ACZPE2_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1319440.1"
                     /translation="MFQQINRLPKAFKNALLFLLIAAFFTLGAAAAEALVIEGSVLSS
                     IDVGKTYQATVKDLRIDENNVRYKSSDTSIIKIGKKDGKITPVAPGKATLTAYNVTNN
                     KSLCNRTITVYLRTESIEVSPKRLYLYTSKPFNSFAFTVTKKFPENSTDTITPQSDNK
                     DIAGAGALTTNISAGDVGKTTIRFYARRTRASSIDSEYSTLMSSTIEVFVHEHNTYST
                     KWTAADSFYHYRACDKDDCPLFRIGTEMDYEEHDFSSYGICRRCRYYKAAKEKDHEHT
                     FNVLNTDETGHFWRCSDADCGATKHYGRHEFKDGNICKVCGHTHEHDAWETGVTVSPD
                     GHWFACSESGCKARKDYTEHTAENESTATCLENLVCTVCGMTFGQGKHGEFAWTTSEN
                     YHTYKCTVCGYWDRDAGSMHVFEYATAETMNCKVCGYVH"
BASE COUNT          329 a          341 c          354 g          409 t
ORIGIN      
        1 tcgtggacat agccgcagac cttgcaattc atggtttcgg cagtcgcata ttcaaaaacg
       61 tgcatcgaac cggcatcgcg atcccaatag ccgcacaccg tgcatttgta ggtatgataa
      121 ttctcgctgg ttgtccaagc aaattcgccg tgcttgcctt gcccaaacgt catgccgcac
      181 accgtgcaga ccaaattctc caagcaggtt gccgtggact cgttttccgc tgtatgctcc
      241 gtataatcct ttcttgcttt acagccgctt tccgaacagg caaaccaatg tccgtccggc
      301 gaaacggtaa cgcccgtttc ccatgcatca tgctcatgcg tatggccgca gaccttgcaa
      361 atgttgccgt ccttgaactc atgccgccca taatgcttgg tcgcgccgca gtcggcgtcg
      421 gagcaacgcc agaaatgacc cgtttcatcg gtgttgagaa cgttaaaggt atgctcgtgg
      481 tctttttctt ttgcggcttt gtaataccgg catcttctgc aaatgccgta gcttgagaag
      541 tcatgctcct cataatccat ttccgtgccg atccggaaca gcggacagtc gtccttgtcg
      601 caggcacggt aatgataaaa gctgtcagcc gccgtccatt tggtgctgta ggtgttatgc
      661 tcatgaacaa atacctcaat agtacttgac ataagcgtcg aatattcgct gtcaatagag
      721 cttgctctgg ttcgtcttgc ataaaagcga atggtcgttt ttccgacgtc gccggcagag
      781 atgtttgtcg ttaaagcacc ggcgcctgca atatctttgt tatccgattg aggcgttatt
      841 gtatcggtcg aattttcggg gaacttcttg gtgacggtaa acgcgaacga attgaacggc
      901 ttgctcgtat agagatagag tctcttgggt gacacctcga tggattcggt acgcagatac
      961 acggttatgg tgcggttgca aagcgactta ttgtttgtga cgttataggc ggtaagcgtc
     1021 gccttgccgg gcgcgaccgg tgtgattttg ccgtcttttt tgccgatttt aataatgctc
     1081 gtgtcgctac ttttgtaccg gacgttattt tcgtctatac gcagatcctt taccgttgcc
     1141 tgataggttt tgcccacatc gatggagctt aatacagagc cttcgatcac aagtgcttcg
     1201 gcagccgccg caccaagcgt gaaaaaggcg gcaataagca gaaaaaggag cgcatttttg
     1261 aatgctttcg gcagacgatt gatttgttga aacatacagc acctcgtttg aaaattatct
     1321 acatatgtat aagaatgtat atgaagttct ttgttataag tataacggag tagccgaagg
     1381 ttgtcaagaa tgaaataggt atgagatttt cacgtgcgct gttctttgct atg
//
LOCUS       DBMDVO010000406         1430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43006_length_1430_cov_0.723100, whole genome shotgun sequence.
ACCESSION   DBMDVO010000406 DBMDVO010000000
VERSION     DBMDVO010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43006_length_1430_cov_0.723100"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            100..465
                     /locus_tag="ACZPE2_06025"
     CDS             100..465
                     /locus_tag="ACZPE2_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR1319441.1"
                     /translation="MNDFVNLLAIFVSSVITSMGLGGGSFYILYLTFFTDTEQFTAQG
                     YNLLLFLPCAVISVIVYVRKKIISFKYIWPIVLGGIAGVIVGYTALSHISSAIVRKMF
                     AAFLLLSGIFSLIKSVKKE"
     gene            542..>1430
                     /locus_tag="ACZPE2_06030"
     CDS             542..>1430
                     /locus_tag="ACZPE2_06030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR1319442.1"
                     /translation="MEYKMLFTPMNIGTVQIKNRVVMPPMMLGFGQFNGCPTKEMMDY
                     YEERAIGGAGLIITEITRVDDFSGASAFAQLAISHDYHIKPLAEMISRVHSHGCKMFI
                     QLHHPGRQNLGLLMGTVPVSIGAERIMGKLYDKMFYKVAPAGKMLMQKDLVLRTFSPS
                     KCDRAYSAESLNREMSVKEIKKIISEFINGAVRAQKAGADGVELHAAHGYLIQQFLSP
                     YTNTRNDEYGGSFEKRMKFLIDIINGIKAACGNDFPVVVRLSVDECYSMIGKPEIGYK
                     LEDGVRMAKVLEKEGIDAID"
BASE COUNT          415 a          246 c          320 g          449 t
ORIGIN      
        1 gattataggt acggttgttt tctcaaagct ttcaaacaga ttcttgtcgg tagcattttc
       61 tttttttatg atctatgccg gaataaggtt attatttaaa tgaacgattt tgtgaatttg
      121 cttgccattt tcgtatcttc ggtaataact tcgatgggtc tcggcggcgg tagtttttac
      181 attttatatt taacattttt tactgacact gagcaattta ctgctcaagg ctataattta
      241 ttgctttttc ttccatgcgc tgttatatcc gtgatagttt atgtcagaaa gaaaattatc
      301 agctttaaat atatttggcc gattgtttta ggcggaattg ccggggttat tgtcggctac
      361 actgcattat ctcatattag cagcgcgatt gttagaaaga tgtttgcagc atttttattg
      421 ctgtccggca tcttttccct aataaagtct gtaaaaaaag aataaatcat tgttaagtgt
      481 ccggttttat gttaagataa aggaaaaaca tttcccgaaa aaagattagg agagtgccaa
      541 aatggaatat aagatgctat tcacccctat gaatatcggt acggttcaaa ttaaaaaccg
      601 tgttgttatg ccccctatga tgcttggttt cggtcagttt aacggttgtc cgacaaagga
      661 aatgatggat tattacgagg aacgtgctat cggaggagcc gggcttatta ttaccgagat
      721 aacgagagtt gatgatttca gtggagcatc ggctttcgcc caacttgcaa tcagccacga
      781 ctatcatatt aaacctcttg cggaaatgat aagcagagtt cactcacatg gttgcaaaat
      841 gttcattcag cttcaccacc ccggcagaca aaatctgggt ttacttatgg gcaccgtacc
      901 ggtatcaata ggtgccgaaa gaataatggg taagctatat gacaaaatgt tttataaggt
      961 tgctcctgcc ggcaaaatgc tcatgcagaa agaccttgtg cttcgtacat tttctccgtc
     1021 aaaatgtgac agagcatact ctgctgagag tttaaacagg gaaatgtccg ttaaggaaat
     1081 caaaaagata atttcagagt ttataaacgg tgcggttcgt gcgcagaagg ccggtgcgga
     1141 cggtgtagaa ttacacgcgg cacacggcta tttgattcag caatttttga gcccatacac
     1201 gaatacgcgg aatgacgagt acggcggaag ctttgaaaaa agaatgaaat tcttaattga
     1261 tattatcaat ggaattaaag ctgcatgcgg caatgatttc ccggttgttg ttcgtttatc
     1321 tgttgatgaa tgttattcta tgatcggtaa acccgaaatc ggctataagc ttgaagacgg
     1381 agttaggatg gcgaaagttc ttgaaaaaga agggattgat gccattgatg
//
LOCUS       DBMDVO010000407         1428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43069_length_1428_cov_1.342125, whole genome shotgun sequence.
ACCESSION   DBMDVO010000407 DBMDVO010000000
VERSION     DBMDVO010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43069_length_1428_cov_1.342125"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="ACZPE2_06035"
     CDS             <1..1366
                     /locus_tag="ACZPE2_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR1319443.1"
                     /translation="TIVDKKGKGKDVYKYTMAVSPLDCMGCGVCVGVCPTNSLKMVAR
                     ETQDAKQAAFDYMVENVTVKEDLANNTVMGSQYKTPLLEFSGSCAGCAETSYARLVTQ
                     LFGDRMYISNATGCSSIWGGPAGTSPYTVDKNGHGPAWANSLFEDNAEHGYGMYLGQE
                     AIRNRLVEEVAAIKDSDKASEDVKAACDEYLATLEDGEKNSAATKKLVDALTAFDCDC
                     EMRKDILEHKEYLSKKSVWVFGGDGWAYDIGFGGVDHVLAQNKDINILVFDTEVYSNT
                     GGQASKASNIGQVAQFAAAGKETAKKSLAEIAMSYGYIYVAQVAMGADQNQTIKALTE
                     AEAYDGPSLVICYAPCEMHSIKGGMTHCQDEMKKAVDAGYWNMFRFNPDLKKAGKNPF
                     TLDSKPASASYRDFIMNEARYSRLTREFPERAEKLFEKAEETATNRYAHLLKLKEMFE
                     PDNK"
BASE COUNT          390 a          348 c          356 g          334 t
ORIGIN      
        1 aacaatagtt gacaagaaag gtaaaggcaa ggacgtttac aagtatacaa tggctgtttc
       61 tccgctcgac tgcatgggct gcggcgtttg cgtaggcgtt tgcccgacca attctcttaa
      121 gatggttgca cgtgagactc aggatgctaa gcaggctgca tttgactaca tggttgagaa
      181 cgttacggtc aaagaggatc ttgcaaacaa caccgttatg ggcagccagt acaagactcc
      241 gcttcttgaa ttctcaggct catgcgcagg ctgcgccgag acttcatatg caagacttgt
      301 aactcagctc ttcggcgaca gaatgtatat ttcaaacgct accggatgct cctctatttg
      361 gggcggcccc gcaggcacat caccctacac cgttgataag aacggtcacg gccccgcttg
      421 ggctaactcc ctctttgagg acaacgcaga gcacggctac ggcatgtatc tcggtcagga
      481 agccattcgc aatcgtcttg tagaagaggt tgccgctatt aaggactccg acaaggcttc
      541 cgaggacgta aaggctgctt gcgacgaata tctcgctact ttggaagacg gcgaaaagaa
      601 ttccgcagca acaaagaagc ttgttgacgc tcttaccgca ttcgactgcg actgcgagat
      661 gagaaaagac attcttgagc acaaagaata cctttcaaag aaatcggttt gggtattcgg
      721 cggcgacggt tgggcatacg acatcggctt cggcggtgtt gaccacgtac tcgcacagaa
      781 caaggatatc aacattcttg tatttgatac cgaggtttac tcaaacaccg gcggacaggc
      841 ttcaaaggct tccaacatcg gtcaggttgc acagtttgct gccgcaggta aggaaaccgc
      901 taagaagagc cttgccgaga tagctatgag ctacggctac atctacgtag cacaggtagc
      961 tatgggtgca gaccagaatc agacaatcaa agctcttaca gaggctgagg cttatgacgg
     1021 tccgtcactc gttatctgct acgctccgtg cgaaatgcac tccatcaagg gcggtatgac
     1081 tcattgccag gatgaaatga agaaggctgt tgacgcaggc tactggaata tgttccgctt
     1141 caaccccgac ctcaagaaag ccggcaagaa tccgttcact cttgattcca agcccgcttc
     1201 ggcaagctat cgtgacttta taatgaacga ggctcgttat tcacgtctta cacgtgagtt
     1261 ccccgagaga gcagaaaagc tctttgaaaa ggctgaggag actgctacaa acagatatgc
     1321 tcaccttctc aagctcaagg aaatgtttga acccgacaac aagtaattaa ggttctgact
     1381 taaaacaaaa cggatactgt atttaaatac ggtatccgtt ttttgttg
//
LOCUS       DBMDVO010000408         1427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43120_length_1427_cov_1.110106, whole genome shotgun sequence.
ACCESSION   DBMDVO010000408 DBMDVO010000000
VERSION     DBMDVO010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43120_length_1427_cov_1.110106"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="rfbF"
                     /locus_tag="ACZPE2_06040"
     CDS             complement(<1..638)
                     /gene="rfbF"
                     /locus_tag="ACZPE2_06040"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR1319444.1"
                     /translation="MKVVILAGGFGTRISEESQFKPKPMVELGGMPIIWHIMKLYSAY
                     GFNEFVICAGYKQHVIKEYFADYFLHTSDITFDFTSGKNEMIVHRNSSEHWKVTVVDT
                     GLNTMTGGRVKRIKEYIGNEPFMLTYGDGVSDVNIAELVEFHKSHGKLVTMSAYNPGQ
                     RFGVLDIDESGRINEFREKTSGDGSLINIGYMVCQPEFIDYIDGDETVLEKKP"
     gene            complement(635..>1427)
                     /locus_tag="ACZPE2_06045"
     CDS             complement(635..>1427)
                     /locus_tag="ACZPE2_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HIR1319445.1"
                     /translation="WDAKTLTSNINLNIACLANEIHETHKKSFGEFRNINTGRDVVVM
                     ASGPSMRYYKPIPGAVHIGMNASFLNPDVDIDYYFTTDYESRAPWFEKLKEYDFVKFF
                     GQYSTGEYRDKFQVSEKIIRENNGRRFFQAAPSEDIHINIEFYPLMAFYSIAFQAIHF
                     ALFTNAKRIYLVGCDCTNAGYFDGSKQRLSDLVAKTSVPHWLDGYRKVKAFVERFYPD
                     TEIISVNPMGLRGLYSDVYTDDFIADHPEIDSSKVTRLNSTQEEK"
BASE COUNT          372 a          323 c          292 g          440 t
ORIGIN      
        1 ggcttctttt caagaacggt ttcgtcaccg tctatgtaat cgatgaattc gggctgacat
       61 accatatagc cgatattgat aaggcttccg tcgccggatg tcttttcacg gaattcattt
      121 attcttccgc tttcatcaat gtcaagaaca ccgaatctct gtccggggtt gtaagccgac
      181 attgttacca gctttccgtg acttttatgg aactcgacaa gctcggctat attgacatcg
      241 gaaacgccgt caccgtatgt cagcataaac ggctcgttgc cgatatactc ctttatacgc
      301 tttacacgtc caccggtcat tgtgttaaga cctgtgtcca ctacggtaac cttccagtgt
      361 tccgagctgt tgcggtgaac aatcatctcg tttttgccgc ttgtgaagtc aaatgtaata
      421 tcggatgtat gcaggaagta gtctgcaaaa tactccttta taacgtgctg tttgtaacct
      481 gcacatataa cgaattcatt gaagccgtaa gctgaataga gcttcattat gtgccatatt
      541 ataggcatac cgccgagttc taccatgggc ttgggcttga actggctttc ttcgcttatt
      601 cttgtgccga aacctccggc gagtattaca actttcattt ttcctcctgt gtgctgttaa
      661 gacgtgttac tttactgcta tctatttcag gatgatctgc tatgaagtca tccgtataaa
      721 cgtctgaata aagtcctctg agacccatag gattaacgga tataatctct gtatccggat
      781 agaagcgttc aacaaacgcc ttgaccttcc ggtagccgtc taaccagtgt ggaacacttg
      841 tctttgctac aagatccgac aatctctgct tgctgccgtc aaaatatccc gcattggtgc
      901 agtcgcaacc aacaagataa attcttttcg cattggtaaa gagcgcaaag tggatagcct
      961 ggaacgcaat tgaatagaat gccataaggg gatagaattc tatattgata tgtatatcct
     1021 cgctgggagc cgcctggaag aatcttctac cgttattctc tctgataatc ttctctgata
     1081 cctggaattt atctctgtat tcaccggtag aatactgacc gaagaacttg acaaaatcgt
     1141 attccttcag cttttcaaac cacggagcac ggctttcgta atcggtggta aaatagtaat
     1201 caatatcaac atcaggattt aagaatgaag cattcatacc tatatgtaca gcaccgggaa
     1261 tgggcttgta gtatctcatc gaaggtcctg atgccattac aacaacatct ctgccggtat
     1321 tgatatttct gaactcgccg aatgacttct tatgagtttc gtgtatctca tttgcaaggc
     1381 aggcaatatt gagattaata tttgaagtga gcgtttttgc atcccat
//
LOCUS       DBMDVO010000409         1426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43175_length_1426_cov_0.965283, whole genome shotgun sequence.
ACCESSION   DBMDVO010000409 DBMDVO010000000
VERSION     DBMDVO010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43175_length_1426_cov_0.965283"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="ACZPE2_06050"
     CDS             <1..300
                     /locus_tag="ACZPE2_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024731.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR1319446.1"
                     /translation="KARVNKAREIQRRRFKGTSVTCNAKMNSALTRKYCIMTTAAANI
                     LRLSFDKLGLSARAYDKVLRVARTIADLDGSDVIDTKHIAEAVQYRSLDRKFWKE"
     gene            complement(364..1248)
                     /locus_tag="ACZPE2_06055"
     CDS             complement(364..1248)
                     /locus_tag="ACZPE2_06055"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1319447.1"
                     /translation="MGKDITLVVMAAGMGSRFGGLKQLEPIGKNGEVILDFSVYDAVK
                     AGFNKVVFVIKHAIEDDFKRIVGSRIEKKVKVEYVFQETDALPEGFTCPADRQKPWGT
                     AHAILCCKNVVKEPFAVVNADDYYGRSAFKKIYDELSSDSEDYCMVGFRLKNTLTENG
                     TVSRGVCVTDNDALESVTEYTKIRSDCSFTEDDGKTWNALSPYTVVSMNLWGFRPDVF
                     KYIEDGFVSFLKEKLNVPKSEYYLPSVVSSLIESGTKKVNVLVAEDKWYGVTYKEDKP
                     AVVASIGELIDKGYYDGI"
BASE COUNT          378 a          368 c          278 g          402 t
ORIGIN      
        1 aaggcaagag tcaacaaggc aagagaaatt cagcgccgcc gttttaaggg gacaagcgtc
       61 acctgcaacg ctaaaatgaa ttctgcactc acaagaaaat actgtattat gaccacggca
      121 gcggccaaca tactgcggct ctcctttgat aagttgggtc tttcggcaag agcctatgac
      181 aaggtgctga gagtggcaag aacaatagcc gaccttgacg gcagcgacgt gatagacacc
      241 aagcatatag ccgaggcggt acaatacaga agtctcgacc gaaaattctg gaaggaataa
      301 ataaacgcaa tacaaaacgg ctgtaagggc aaatcttaca gccgttaatt ttattgttct
      361 tttttatatt ccgtcgtaat agcctttgtc gataagctcg cctatcgaag ctacaacagc
      421 cggcttatcc tccttatacg ttacgccgta ccacttatcc tcggcgacga gaacattaac
      481 cttttttgtt ccgctctcga taagagaaga tacgacggac gggagataat actcggattt
      541 cggcacattc agcttttcct taagaaaaga aacaaaccca tcctctatgt atttgaatac
      601 gtcgggacga aatccccaaa ggttcatgga aacgaccgta tacggcgaca acgcattcca
      661 agtcttgccg tcatcctcgg taaatgagca atcgctgcga atttttgtat attcggtgac
      721 gctctctaag gcgtcattat ctgtaacaca aacgcctctt gaaacggtac cgttttccgt
      781 aagcgtattc ttgagtctga agccgaccat acaataatcc tcggaatccg atgacagctc
      841 gtcgtaaatc ttcttaaatg cgcttctgcc gtaataatca tcggcattta caaccgcaaa
      901 cggctctttt acaacatttt tgcagcagag aattgcatga gcggttcccc acggcttttg
      961 acggtctgca ggacaggtaa agccctccgg aagagcatct gtctcctgaa aaacgtactc
     1021 aaccttaacc tttttttcta tacgcgagcc gacgatacgt ttaaaatcat cctcgattgc
     1081 gtgttttata acaaaaacaa ccttattaaa gcctgccttt accgcatcgt aaaccgaaaa
     1141 atcaagaatt acctctccgt tttttcctat cggctcaagc tgctttaatc cgccgaaacg
     1201 actgcccata ccggcagcca taacaaccaa agttatgtcc ttacccattg tttttctcct
     1261 ttagccccat tggcttgttt tatttattat ttgcctcttt ttcggtttgc tcggctattg
     1321 cctcgtcctt ttcagcctct ttttcggctt ccttcgcctc tttttcggct tcctgtgccg
     1381 ccttttcggc tttatcagtc ttaatttctt ccgctgcctt acgagc
//
LOCUS       DBMDVO010000410         1424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43249_length_1424_cov_1.093726, whole genome shotgun sequence.
ACCESSION   DBMDVO010000410 DBMDVO010000000
VERSION     DBMDVO010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43249_length_1424_cov_1.093726"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="ACZPE2_06060"
     CDS             complement(<1..1122)
                     /locus_tag="ACZPE2_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319448.1"
                     /translation="MTHDNGAKVLLLEREARLGGILKQCIHDGFGLHHFGEQLSGPEY
                     AERFEDELYKRNIEYSTLTFVTKIDKNGDGSFSVHTVGRSGVSIFTAKALVLATGCRE
                     RTAKQVFIHGTRPAGVFTAGTAQHFTNLLGLMPAKKCVILGSGDIGLIMARRLTLEGA
                     KVLGVYEAKPTPSGLTRNLHQCLHDYDIPLHVSHTVTRVFGVDRLEAVEISKVDENMA
                     PIPGTQSRVECDTLILSVGLIPENELAESVGVKLDPRTKGPVCDGQCMTSIDGVFSCG
                     NALHVNDLVDFVSQNAEQAGKSAAAYSAKERRLIEITPGDGLLYAVPQRLNLNEDNSK
                     TDLYFRVREEENNCVLHIYADGKEILKKRYAFMRPPEMEN"
     gene            complement(1138..>1424)
                     /locus_tag="ACZPE2_06065"
     CDS             complement(1138..>1424)
                     /locus_tag="ACZPE2_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1319449.1"
                     /translation="AENPDYGEIICRCEQISKGEILDALKSPICVPTVDGIKKRIRPG
                     MGRCQGGFCSPLVTKIIAEFLGVPLYEVKKSSAEAVITYGETKVNEGGEE"
BASE COUNT          308 a          388 c          354 g          374 t
ORIGIN      
        1 attttccatt tcgggcggac gcataaatgc gtaacgcttt ttaagtattt ctttaccgtc
       61 cgcgtaaata tgaagcacac agttgttttc ctcctcgcgg acgcggaaat acaggtctgt
      121 tttactgtta tcctcgttga gattgagcct ttgcggcact gcgtacaata agccgtcacc
      181 cggggttatc tcaataagcc gcctttcctt ggcggaataa gcagcggctg attttcccgc
      241 ctgctcggcg ttctgcgaaa caaagtcaac caaatcgttg acgtgcaaag cgttaccgca
      301 gctgaatacg ccgtcgatgc tcgtcataca ctgaccgtcg catacggggc cctttgtgcg
      361 tgggtcaagc tttactccga cggactcggc gagctcgttt tcgggaatca atccgaccga
      421 gagaatcagc gtgtcacatt cgacgcggct ctgtgtgccg ggtatcggag ccatattttc
      481 gtcaaccttt gaaatttcaa ctgcttcaag cctgtcaact ccgaacacgc gagtgacggt
      541 gtgcgacacg tgcagcggaa tgtcataatc gtgcaggcat tgatgcaggt ttctcgtaag
      601 tcccgacggc gtaggctttg cctcataaac gccgagaacc tttgcgccct caagcgtaag
      661 ccggcgcgcc atgataagtc ctatatctcc cgagccgaga attacgcatt ttttggcggg
      721 cataagcccg agcagatttg taaaatgctg tgcggttccg gccgtgaaaa cgcctgcagg
      781 acgcgtaccg tgaataaata cctgcttggc ggtacgctcg cgacagcccg tagccagcac
      841 aagcgctttt gccgtaaata tcgaaacgcc gcttctgccg acggtatgca ccgaaaagct
      901 tccgtcgccg tttttgtcta ttttcgtgac aaacgtcagg gtgctgtatt cgatattgcg
      961 tttgtacagc tcatcctcaa accgctcggc gtattctgga ccggagagct gttcgccgaa
     1021 gtgatgaaga ccgaaaccgt cgtgaatgca ctgcttgagt attcctccga gccttgcctc
     1081 tctttcaaga aggagaacct tggcaccgtt atcatgagtc atagttacct gtcgtcatca
     1141 ctcttcgcct ccttcgttta cctttgtttc gccgtatgtt atcaccgctt ccgccgagga
     1201 ctttttaacc tcatacagcg gaacgccgag gaattcggca attattttgg tgacaagcgg
     1261 cgaacagaag ccgccctgac aacgtcccat tcccggtctt atgcgttttt tgataccgtc
     1321 aactgtcgga acgcatatcg gggatttcaa agcgtcgaga atttcgccct tgctgatttg
     1381 ctcgcagcgg caaataatct cgccgtagtc gggattttcg gcta
//
LOCUS       DBMDVO010000411         1423 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43275_length_1423_cov_1.549168, whole genome shotgun sequence.
ACCESSION   DBMDVO010000411 DBMDVO010000000
VERSION     DBMDVO010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43275_length_1423_cov_1.549168"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1423)
                     /locus_tag="ACZPE2_06070"
     CDS             complement(<1..>1423)
                     /locus_tag="ACZPE2_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1319450.1"
                     /translation="KEVFRDVADITDYSGNLVLSFVDYRMDDAPKYTVKECKERDATY
                     AAPLRVTARLLNKETGEIKESEVYMGDFPLMTDSGTFVINGAERVIISQLVRSPSVYY
                     GMTHDKTGKELYSTTVIPNRGAWLEYETDQNDLFYVRIDKNRKIYITTFIRALGLSSN
                     EEILDFFGYDERIKATLEKDTTINTEQALIEVYKRLRPGEPPTLETAQAQIDNLFFDP
                     HRYDLSRVGRYKYNKKLAISARIAGFKADEPIISNLTGEILAEPGETITPELAYRIEQ
                     EGVNTAVIVTPEDKTVKVYSNGMVDIGEYVPMFTEAELEDIGINEKVRFTVLQEVLEK
                     CGDDKDALSEELSARCDDLIPKHIIIDDIMASINYLNCIADGVGTTDDIDHLGNRRIR
                     SVGELLQNQFRIGFSRMERVVKERMTLQAQEPDKVTPAALINIRPVMAAIKEFFGSSP
                     LSQFMDQTNPLAELTHKRRLSALG"
BASE COUNT          343 a          337 c          340 g          403 t
ORIGIN      
        1 ccgagagcgg aaagtcttct cttatgagta agctctgcga gggggtttgt ctgatccatg
       61 aactgagaaa gcggagacga accgaagaac tcttttatag ccgccataac ggggcgaata
      121 tttatcaaag cggcgggagt taccttatcg ggctcctgcg cctgaagagt cattctttct
      181 tttacaactc tctccattct ggaaaagcct attctgaact gattctgaag aagctcgccg
      241 acggaacgga tacgacggtt gccgagatgg tctatgtcat ccgttgtacc tacgccgtcc
      301 gcaatgcagt tgaggtagtt gatagaagcc attatatcat caattatgat atgcttcgga
      361 atgaggtcgt cgcaacgagc cgaaagctct tcggaaagcg catccttatc gtcgccgcac
      421 ttttcgagca cttcctgaag cacggtaaag cgtaccttct cgttaatgcc gatatcctca
      481 agctcagcct ctgtgaacat gggaacatac tcgccgatgt caaccatacc gtttgaatag
      541 accttaacgg ttttatcctc cggagttact ataacggcgg tattaacgcc ctcctgctct
      601 attctgtaag caagctcggg ggttatggtt tcgcccggct ccgcaagtat ctcgccggta
      661 aggttgctga taatcggttc atcagcctta aagccggcta tgcgagcgga gattgcaagc
      721 tttttattgt atttatagcg acctactctt gaaaggtcat atctgtgcgg atcgaagaag
      781 agattatcaa tctgtgcctg agcagtctcg agtgtgggag gctcgccggg acgcagtctc
      841 ttgtatactt cgataagagc ctgctcggta tttatggtag tatccttttc gagagtagcc
      901 ttgattctct cgtcatagcc gaaaaagtca agtatctctt cgtttgagga aagtccgagt
      961 gctctgataa aggtagtgat gtaaatcttt ctgttcttat caattcttac ataaaacagg
     1021 tcgttctggt cagtttcgta ctcgagccat gcgcctctgt tggggataac ggtagtcgag
     1081 tagagctctt tacccgtttt atcatgcgtc ataccatagt acacgctggg agaacggaca
     1141 agctgcgaaa taataacgcg ctccgcaccg ttgattacga aggtaccgct gtctgtcatg
     1201 agcgggaaat cgcccatata gacttcgctt tctttaattt cgcccgtttc tttgttgagc
     1261 agccttgcag taactcttaa aggagcagca tatgtcgcgt ccctctcttt acattccttg
     1321 acggtatatt tgggagcatc gtccattctg taatcgacaa agctcaaaac aaggttgccg
     1381 gaatagtccg taatatctgc gacatcgcgg aatacttcct tca
//
LOCUS       DBMDVO010000412         1423 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43312_length_1423_cov_0.690620, whole genome shotgun sequence.
ACCESSION   DBMDVO010000412 DBMDVO010000000
VERSION     DBMDVO010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43312_length_1423_cov_0.690620"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="ACZPE2_06075"
     CDS             complement(<1..632)
                     /locus_tag="ACZPE2_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1319451.1"
                     /translation="MMGEKQWGSRIGFLLATAGAAIGLGNLWKFPYLMGENGGFTFLV
                     VYLIFVLILGLPCMMLEMSLGRYTGRDPIQSYKMVNPKAKIVGIFGVLAPFIILSYYS
                     VIGGWLIKYVVSYVTTFNAPADFGTFIASPTTVIWHFIFMAMVIYVCYKGIGGIEKAS
                     KIMLPALFVLLIIIIFRSVSLKGSMEGIKFIFAPKKGFSLASVNAALGQV"
     gene            complement(840..>1423)
                     /locus_tag="ACZPE2_06080"
     CDS             complement(840..>1423)
                     /locus_tag="ACZPE2_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015962.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR1319452.1"
                     /translation="IYPVGALETAFRLKEENPDIILVGERNEIIVPGFDYGNSPANIE
                     HTDFIGKTIVHTTSAGTQGIVNAVNADEIITASFVNAPAVAKYLKKKNPETVSFVCMG
                     KCAVEPAEEDTWCAEYISSLITGTPYNVEERLKELRNLEGRRFFRPDNQLQCPERDFY
                     LATQIGLFDFVLKADCIDKAVPLYRLNFADKTR"
BASE COUNT          420 a          304 c          285 g          414 t
ORIGIN      
        1 aatacctgtc cgagagcggc attaacgctc gcaagggaaa agcccttctt cggagcaaat
       61 ataaacttga ttccttccat agagcctttg agtgaaaccg aacggaaaat gattataata
      121 agaagcacaa aaagagccgg aagcatgatt ttgcttgctt tttcaattcc gcctatgcct
      181 ttgtagcata catagataac cattgccatg aatatgaaat gccatataac ggttgtcggg
      241 cttgctatga atgtgccgaa atcagccggt gcattaaagg ttgttacata gctcacaacg
      301 tatttgataa gccagccgcc tattacgctg taatagctga gaatgatgaa cggagcaaga
      361 actccgaaga tacctacgat ttttgctttc ggattgacca ttttgtagct ctgtatagga
      421 tctcgccctg tgtatcgtcc aaggctcatt tcgagcatca tacagggcag accgagaata
      481 agtacgaaaa tgagatatac aacaagaaat gtaaatccgc cgttttcgcc catcagatac
      541 gggaatttcc aaaggttgcc cagacctatg gctgcacccg cagttgcaag cagaaagcct
      601 attctgctgc cccattgttt ttctcccata attaattcct cctaaattaa tataacactg
      661 cgccgtaaaa tcggttcact gccattaaac cgatttttta taataattta ttatatcata
      721 taaaggagtt ttttaaaagg cagtaccaat gtcaataaaa accatataaa ccgtaatgtt
      781 tatatgaata aacgaaataa aagcattcca agttaatgaa atgcttttaa aaatatgctt
      841 tatctggttt tatccgcaaa attaagccta taaagcggaa ctgctttatc tatacagtcg
      901 gctttaagaa caaagtcgaa caatccgatt tgtgttgcaa ggtaaaaatc acgttccggg
      961 cattgaagct ggttatccgg tctgaaaaat cttctgcctt caaggtttct cagttccttg
     1021 agcctttcct ctacgttata gggagtgcct gtaatcaaag aggaaatgta ttcggcacac
     1081 caggtgtcct cttccgcagg ctcaacagca catttgccca tacatacgaa ggaaaccgtt
     1141 tcgggatttt ttttctttaa atactttgca acggccggag catttacaaa ggacgccgtt
     1201 attatttcgt cagcattaac tgcgtttaca atgccctgtg ttccggcact tgttgtgtgg
     1261 acgattgttt tgccgatgaa atccgtatgc tctatgttgg caggagagtt tccgtagtca
     1321 aagccgggaa cgataatttc attccgttca ccgacaagta ttatgtcggg attttcttcc
     1381 ttaagcctga aggctgtttc cagcgcaccg acgggatata tac
//
LOCUS       DBMDVO010000413         1422 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43358_length_1422_cov_0.956851, whole genome shotgun sequence.
ACCESSION   DBMDVO010000413 DBMDVO010000000
VERSION     DBMDVO010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43358_length_1422_cov_0.956851"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1422)
                     /locus_tag="ACZPE2_06085"
     CDS             complement(<1..>1422)
                     /locus_tag="ACZPE2_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR1319453.1"
                     /translation="GADNDWYCIYSGTNNQKADVTADFGYNVTASKIRISITDISNKT
                     GAAITSTDGTKTIPAGAPTYLFLDGVTVLQPKADYKNITVEQKDDEIAIDAAKLTGYQ
                     GGKVREGIKQLMDGNIGNKVILNGNTTLDFGQQVTFSGFRYYPHVGATANDVVKSFGI
                     DGGVKIYSELGDEGSADTLLANAERISLSKADIDYTAADGGTDYTQPIDVDFGKNVTA
                     RGINFNIWHVPGKTEAESPYAGEIRLLAAKDYSHGYALKNDVVKFGETPDVIEKGASK
                     PGAGYVWDNPSCLFDGVIPIKNGDFGIDKNSSTYYVGFGTYGADAGFMSGEKAWLTID
                     LKQETDFSAVRLFGRMCYAMSPTEVNLYFSNDNSNWTLASHNTDSLNGKKIDDMFCYS
                     KSGDIYTDMKPNDGDSNYSVRARYIKIEILKAGNSDYATLSEIMLVKPGAEVLTCDAL
                     NEKILKNSVKTAIKTVQSGVLDGT"
BASE COUNT          321 a          379 c          289 g          433 t
ORIGIN      
        1 gtgccgtcaa gcacaccgct ctgcaccgtc tttatcgcgg tcttgaccga atttttgaga
       61 attttctcat tgagagcgtc acaggtcaaa acctcagctc ccggcttcac taacataatc
      121 tcggagagcg tagcataatc cgaattgccc gccttcaata tttcaatctt tatataacgt
      181 gcgcggacgc tgtaattgct gtctccgtcg ttgggcttca tgtccgtata tatgtcgccg
      241 gatttggaat agcaaaacat atcgtcgatt ttcttgccgt tcaaactgtc tgtattatgc
      301 gacgcaagcg tccagttaga attgtcgtta ctgaaataga ggtttacctc tgtcgggctc
      361 attgcgtaac acattctgcc gaaaagcctt actgccgaaa agtctgtttc ctgcttaagg
      421 tcaattgtca accacgcctt ttctccgctc ataaagccgg cgtcggcgcc gtaggttccg
      481 aatccgacat aataagttga gctgttttta tctattccga aatctccgtt tttaatcggt
      541 ataactccgt caaacaggca cgacggattg tcccagacat aacccgcgcc cggtttgctc
      601 gctccttttt cgattacatc cggggtctcg ccaaacttaa ccacatcgtt cttcaatgca
      661 tatccgtgag aataatcctt agccgcaaga agtctgatct cgcctgcata tggagattcg
      721 gcttctgttt tacccggaac atgccagatg ttgaagttga ttccgcgggc ggtaacgttt
      781 ttcccgaaat caacatctat cggctgcgtg taatccgttc ctccgtcagc agccgtataa
      841 tcaatatccg ccttcgacag cgaaatcctc tcggcattcg cgagaagcgt atcggccgac
      901 ccttcatctc cgagctcgct gtatattttt acccctccgt caattccgaa gcttttaaca
      961 acatcatttg ccgttgcgcc cacatgaggg taatatctga atccggaaaa ggtcacctgc
     1021 tgtccgaaat cgagtgtcgt attaccgttc aggattactt tattgccaat attaccgtcc
     1081 ataagctgct tgatgccttc tctcacttta ccgccttgat atccggtcag cttagccgca
     1141 tctattgcaa tttcgtcgtc tttttgctca accgttatgt ttttataatc tgcttttggt
     1201 tgcagaaccg ttacgccgtc taagaaaaga tatgtcggag ctccggccgg aatagtcttt
     1261 gtcccatcag tcgatgttat cgccgccccg gttttatttg atatatccgt aatgcttatt
     1321 ctgatctttg aagcagtgac attatatccg aagtccgctg tcacatccgc cttttgattg
     1381 tttgtacccg aataaatgca ataccagtca ttatccgcgc cg
//
LOCUS       DBMDVO010000414         1420 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43465_length_1420_cov_0.955269, whole genome shotgun sequence.
ACCESSION   DBMDVO010000414 DBMDVO010000000
VERSION     DBMDVO010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43465_length_1420_cov_0.955269"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="ACZPE2_06090"
     CDS             complement(<1..1148)
                     /locus_tag="ACZPE2_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014877.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR1319454.1"
                     /translation="MLDGKTVFPLSEICDGIYSNSELPCGKYNITPPVFIGENTEISD
                     GADLGPYTVVGDGCFIGEKAFVRGSIMLNKSAALRGADISGAVMGVNSVAEENSKMSL
                     GSVLCEKTTVGRNMAVGENVKVTPKPHGGISAPKSQPQAYYYGENITALGSRGTDSLF
                     GDFDIGLFCKVGRALGSCEFGTRTGIGYDDSVSSAAAVKAVTAGLISSGSHVFDFGRC
                     FLSEVAFFSSFCSLGCGIYIYSEKNGIHSAFYGNGGLPLSPDFEQELERRAENGEFRR
                     CSAQNMRAVSDMSSMSSMYRRELLRQSGDMLSGISANVMSDNKDILLLMEDCLLSLGC
                     GKGDDITFKITRGGTEVSAFCRECGWINMDKLLSLCCLYEFQCGHDVAV"
BASE COUNT          389 a          382 c          294 g          355 t
ORIGIN      
        1 acagcaacat catgaccaca ttgaaactca tacagacagc acaacgaaag cagtttatcc
       61 atattgatcc acccgcattc acggcaaaat gccgaaacct ccgtgccgcc tcttgttatc
      121 ttaaacgtga tgtcatcgcc tttaccgcag ccgagtgata aaagacaatc ctccataagt
      181 aaaagtatgt ccttattatc gctcattaca ttggccgaaa tacccgaaag catatctccc
      241 gattgtctca aaagttctcg gcgatacata ctgctcatgc ttgacatatc ggagacggct
      301 cgcatattct gtgccgagca acggcgaaat tcgccgtttt ctgcacggcg ctcaagttcc
      361 tgctcgaaat cgggcgataa cggcaatccg ccgtttccgt aaaacgccga atgaataccg
      421 ttcttttccg aataaatgta aattccgcag ccgagagaac aaaaagaaga gaaaaaagcg
      481 acttctgaca aaaagcatct gccgaaatca aacacgtgcg agcccgagga tataagtccc
      541 gccgtaacgg cctttaccgc cgctgcggaa gaaacgctgt catcgtaccc tattcctgtt
      601 cgcgtaccga attcacagct gcccaaggct ctgccgactt tgcaaaacag gcctatatca
      661 aaatcgccga aaagcgaatc ggtaccgcgg ctgcctaaag cggtaatatt ttcgccgtaa
      721 taataagcct gcggctgact tttgggcgcg gaaataccgc cgtgcggttt cggtgtgact
      781 tttacatttt cgcccaccgc catatttctg ccgacagtcg ttttctcaca cagcacgctg
      841 cctaaagaca ttttgctgtt ttcctccgca accgaattta cacccataac ggctcccgat
      901 atatcggctc cgcgtaaggc tgcggattta ttgagcataa tactgccgcg aacaaatgct
      961 ttttcgccta taaaacagcc gtcgcccact acggtatacg gtcctaaatc cgctccgtcc
     1021 gaaatttcgg tattttcacc tataaacaca ggcggcgtaa tattgtattt gccgcaaggc
     1081 agttcgctgt ttgaatatat gccgtcgcat atttccgaca gaggaaatac tgtttttccg
     1141 tcaagcatat cgcgagtaca ttttaaatat gataataccg tatttatacc gcgcctgtaa
     1201 ccggcgcacc gaaatgcaaa aagacttttg ttttgtgaga gcaattcagg cagtatctca
     1261 tttgaaaaag ccgtgattgt gcggtcgcga agcattttta atattttgcc ggaaattata
     1321 taaatgcccg tgtccgcctt atccgtaaga aagccgcacc tgccgacgct ccccgaaaag
     1381 cccgtaattc tgccggacgg tgacgtgaaa acaagcttct
//
LOCUS       DBMDVO010000415         1414 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43678_length_1414_cov_1.476771, whole genome shotgun sequence.
ACCESSION   DBMDVO010000415 DBMDVO010000000
VERSION     DBMDVO010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43678_length_1414_cov_1.476771"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(46..>1414)
                     /gene="infB"
                     /locus_tag="ACZPE2_06095"
     CDS             complement(46..>1414)
                     /gene="infB"
                     /locus_tag="ACZPE2_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1319455.1"
                     /translation="VDVNGSPITFLDTPGHEAFTSMRARGAMVTDIAILVVAADDGIM
                     PQTIESINHAKAAGVSVIVAINKMDKPDASPDRVLQQLTEYDLVPEEWGGDVICVPVS
                     AKTHMGIDKLLESILLVAEMLELKANPNRAAKGIVIEAKLDKGRGPVATLLVQNGTLR
                     TGDIVVAGTTVGRVRVMTNERGKKLEEAGPSVPVEVMGLDEVPTGGDKFDAVSDEKLA
                     RELVEQRKHEQKEEQFKQFQKVTLDNLFSSINEGELKELNIIVKADVQGSVEAVRQSL
                     EKLSNDEVKVKVIHGGVGAVSESDVMLAAASNAIIVGFNVRPDSVAESIAERDGVDMR
                     MYRIIYDCIEEISDAIKGMLAPKYREVTLGKAEVRNVFKLSSAGMIAGSYVLDGKITR
                     NSQIRVVRDGIVVSEDSIASLKRFKDDVKEVQSGYECGIGLEKFNDIKEGDILEAFVM
                     EEYKD"
BASE COUNT          302 a          397 c          323 g          392 t
ORIGIN      
        1 ttttctgtat cctgccatag cttaattcct ttcatataaa aggaatcagt ccttgtattc
       61 ctccataacg aatgcctcga ggatgtctcc ctctttaatg tcgttgaatt tctcaagacc
      121 gattccgcat tcgtagccgg actgaacctc ttttacgtcg tccttaaagc gctttaagga
      181 tgcgatgctg tcctccgaaa caactatgcc gtcgcggaca acacgaattt gtgaatttct
      241 tgtaattttg ccgtcaagaa cgtagctgcc ggctatcata ccggcggagg agagcttaaa
      301 tacgtttctt acctctgcct tgccgagcgt tacctcacgg tacttcggtg cgagcatgcc
      361 cttaattgcg tcgcttatct cttctatgca gtcatagatt attctgtaca ttctcatatc
      421 tacgccgtcg cgctccgcta ttgactcggc tacggagtcg gggcggacgt taaagcctac
      481 tataatcgca tttgaagccg cggcaagcat tacgtcggat tcgcttacgg caccgacgcc
      541 gccgtgtata acttttacct tgacctcatc gttcgagagc ttttcaagac tctgtcttac
      601 agcctctacg gagccctgaa catctgcctt gacaatgatg ttaagctcct taagctcacc
      661 ctcgtttatg gatgagaaga gattatcaag agttaccttc tggaactgct tgaactgctc
      721 ctccttttgc tcgtgctttc tctgctcaac aagctcacgt gcaagctttt cgtcggaaac
      781 ggcgtcaaat ttgtcaccgc cggtcggaac ctcatcaagg cccataacct ctacgggtac
      841 ggacgggcct gcctcttcaa gctttttgcc gcgctcgttt gtcataactc tgactctgcc
      901 cacggtcgtt ccggcaacta cgatgtcgcc cgtcctcaac gtaccgtttt gtacaaggag
      961 cgtcgcaacg gggcctctgc ccttatcaag cttagcctct attacaatac cctttgcggc
     1021 acggttcgga tttgctttaa gttcaagcat ttccgcaacg agaagtatcg actcgagaag
     1081 cttgtcgatg cccatatgag tcttagccga aacgggaaca catattacat cgccgcccca
     1141 ttcctcggga accaggtcat actcggtgag ctgctgaaga actctgtccg gtgacgcgtc
     1201 cggcttatcc attttgttta tggctacaat tacgctgact cccgcggcct tcgcgtggtt
     1261 tatggattca atggtctgag gcatgatgcc gtcgtccgcc gcgacaacga ggatcgcgat
     1321 atcggtgacc atagcgcctc tcgcgcgcat ggaagtgaaa gcctcgtgac ccggagtgtc
     1381 aaggaacgtg atagggctgc cgttcacatc aacg
//
LOCUS       DBMDVO010000416         1412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43767_length_1412_cov_1.270786, whole genome shotgun sequence.
ACCESSION   DBMDVO010000416 DBMDVO010000000
VERSION     DBMDVO010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43767_length_1412_cov_1.270786"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="ACZPE2_06100"
     CDS             <1..1206
                     /locus_tag="ACZPE2_06100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1319456.1"
                     /translation="SGGVVFGVKKLSLQKLYAAYADDSPYIEDNFADPKKVKMTFPEK
                     KRNLIHIYLESMENSYFSKELGGYMDTNLLPDLGKLSKEGYNFSDLPEGFGGNPSSTG
                     ANWSVASMVNMSSGLPMKVPVGANAYGSADNFLPGATTIGDILKEQGYNQTVMFGADA
                     SFGGLDYFFEEHGDYNIMDYKRAKQDNFIPQDYSVWWGYEDDKLYEYAKMELTRLAGE
                     DKPFYFVMETADTHFPDGYLSPNAKTPFDKQYANVIFYSQAETVKFVRWIQSQPFYEN
                     TTIVINGDHLSMDPNFFKDFDPNYRRTCFNLILNPTPNCTSAPDTRFHNREWATFDML
                     PTMLASIGVEIEGNKLGIGTNLFSDTPTLFERDGTDFVNEELEKRSNFYNEHILVDWS
                     RVGGDKKDK"
     gene            complement(1285..>1412)
                     /locus_tag="ACZPE2_06105"
     CDS             complement(1285..>1412)
                     /locus_tag="ACZPE2_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1319457.1"
                     /translation="EIPVMRLGTPEDVANAVSFLADDKSSYITGQIISVNGGIVI"
BASE COUNT          392 a          320 c          321 g          379 t
ORIGIN      
        1 tccggcggcg tggtgttcgg tgtaaaaaag ctttctcttc aaaagctcta tgcggcttat
       61 gctgatgatt cgccgtacat agaagataat tttgccgatc ccaaaaaggt taaaatgacc
      121 ttcccggaga aaaagcgcaa ccttatccat atttatctcg aatctatgga gaactcgtat
      181 ttctcaaaag agctcggcgg atatatggac acaaatcttc ttcccgacct tggaaagctc
      241 tcaaaggagg gctacaattt ctcggatctt cccgagggct tcggcggtaa tccgtcctcg
      301 acgggcgcca actggtctgt ggcttcaatg gtgaatatga gcagcggctt gcccatgaag
      361 gtgccggtcg gtgcaaatgc atacggctct gccgacaatt tcctgccggg agctaccaca
      421 ataggtgata tattaaaaga acagggctat aatcaaaccg ttatgttcgg tgcagatgca
      481 tccttcggcg gacttgacta cttctttgag gagcacggcg attacaatat aatggactac
      541 aagcgtgcga aacaagataa cttcattcct caggattaca gcgtttggtg gggctatgag
      601 gatgataagc tctatgaata tgcaaagatg gagcttacac gcttggcagg cgaagacaaa
      661 ccgttctatt tcgttatgga aacggctgat acgcacttcc cggacggcta cctttctccc
      721 aatgccaaga ctccgtttga taagcagtat gcaaacgtaa tattctacag ccaggcagag
      781 acggttaagt ttgtacgttg gatacagtct cagccgttct atgagaacac taccatagta
      841 attaacggcg accaccttag tatggatccg aatttcttta aggactttga cccgaattac
      901 agacgtacat gctttaacct tatacttaat cccacaccta actgcacctc ggctcccgat
      961 actcgcttcc ataaccgcga gtgggcaacg tttgatatgc tgccgacaat gctcgcaagt
     1021 atcggcgttg agatagaggg caataagctc ggtataggaa caaacctctt ctctgataca
     1081 ccgactctct ttgagcgtga cggaaccgat tttgtaaacg aagagcttga aaaacgaagc
     1141 aatttctata acgagcatat tcttgtcgat tggtcaagag taggcggcga taaaaaagac
     1201 aaataataaa ttcgggatgt aaaacttatg ttttacatcc cgttttttgc ttatacgatt
     1261 tttgtatact gaaccttgta attactaaat aactatgccg ccgtttacgg agattatttg
     1321 ccccgtgata tatgacgatt tgtcatcggc taagaatgaa accgcatttg ccacgtcttc
     1381 gggagtaccg agccgcatta cggggatttc tt
//
LOCUS       DBMDVO010000417         1409 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43919_length_1409_cov_1.078746, whole genome shotgun sequence.
ACCESSION   DBMDVO010000417 DBMDVO010000000
VERSION     DBMDVO010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43919_length_1409_cov_1.078746"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="ACZPE2_06110"
     CDS             <1..324
                     /locus_tag="ACZPE2_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR1319458.1"
                     /translation="KDVSESGGYGVIFGNTLSKNELSAIKEAVKTSPRRFIAQEVIDF
                     KGLKVFENGRQVERKADLRAFVLYGDEIKVWHSGLTRYSQDPDSFVVNSSQGGGFKDT
                     WVLSR"
     gene            306..998
                     /locus_tag="ACZPE2_06115"
     CDS             306..998
                     /locus_tag="ACZPE2_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR1319459.1"
                     /translation="MGIISIKSTDNLFWLGRYVERVFTTLRVFSEYYDKMIDKDENAY
                     VDFCRKLGIENTYSYKQEFITKYLFDENDPNSVMSNLLCAYDNAVVMRNEISSETLSY
                     IQMAVNYMEQGRESSAPMLKLQEVFDCIFAFWGSADDFVESETTRNILKFGRSVERLD
                     LYTRFSFSPTLIKKEFSILLNRLYKVGVDCNIDAINTLMNIILEKDEYSDYDLYTVRD
                     ELSKVFITAPQY"
     gene            1018..>1409
                     /locus_tag="ACZPE2_06120"
     CDS             1018..>1409
                     /locus_tag="ACZPE2_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019977.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HIR1319460.1"
                     /translation="MKHIKFFYSMKIDFSSPVIRHCFSLRCLPFDTPSQRIELINVDI
                     EPHNMLTETADGFGNRVLTDRIIEPHSKFVAIVEGKASLDFSKREKEELNCIYKYPSQ
                     HTVPGEKIIELVSGLGDMANLTALETAQA"
BASE COUNT          428 a          296 c          322 g          363 t
ORIGIN      
        1 aaagatgtat cggagtccgg aggctacggg gtcatattcg gaaatactct gtctaaaaac
       61 gaactctctg ccataaaaga agccgttaaa acgtcgccgc gccgatttat agcgcaggag
      121 gtaatagact ttaaagggct caaggttttt gaaaacggca ggcaagtcga aaggaaagcg
      181 gatcttcgcg cgtttgtgct ctacggcgac gaaataaaag tttggcacag cggtcttaca
      241 cgctattctc aagatccgga ttcttttgtt gtgaactcat ctcagggagg aggatttaaa
      301 gacacatggg tattatctcg ataaaatcca cggacaatct cttttggctc ggcagatatg
      361 tggagcgcgt ctttaccact cttcgcgttt tttcggaata ttacgataaa atgatagaca
      421 aagacgaaaa cgcctatgtt gatttttgcc gcaagctcgg tatagaaaac acatattcat
      481 acaagcagga atttataacg aaatatcttt ttgatgaaaa cgacccaaat tctgttatga
      541 gtaatttact atgcgcctat gataacgccg ttgttatgcg taacgagata agctccgaaa
      601 cgctgtcata tattcaaatg gcggtaaatt atatggagca aggcagagag agcagcgcac
      661 cgatgctcaa attgcaggag gtatttgact gcatatttgc cttttggggc agcgccgacg
      721 actttgttga atcggagaca acgagaaata tactgaagtt cggcagaagc gttgaaagac
      781 ttgacctcta tacccgtttt tccttttctc cgactctcat caaaaaggaa ttcagtatac
      841 ttttaaaccg tctttacaaa gtcggcgtag actgtaacat agacgcgatc aacaccctta
      901 tgaacatcat tctcgaaaaa gatgagtaca gcgactacga cctatatacc gtgcgcgacg
      961 agttgtctaa ggtatttatt acggcaccgc agtattgatc aaaaaaggaa gataaacgtg
     1021 aagcacatca aattttttta cagtatgaaa attgattttt cgtcccctgt gataaggcac
     1081 tgcttttctt tgagatgctt gccgtttgat acgccgtcgc agaggataga gctcataaac
     1141 gtggacatag agccgcataa catgcttacc gaaacggccg acggcttcgg caacagagtc
     1201 ctcaccgaca gaattatcga gcctcactca aaattcgttg caatagttga gggaaaagcg
     1261 agtttagact tttcaaagcg cgaaaaggaa gagcttaact gcatttataa atatccgtcg
     1321 cagcacacgg tacccggaga aaaaatcatt gaactcgtgt cggggctcgg tgatatggca
     1381 aaccttaccg cgcttgagac cgcacaggc
//
LOCUS       DBMDVO010000418         1409 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43924_length_1409_cov_1.005352, whole genome shotgun sequence.
ACCESSION   DBMDVO010000418 DBMDVO010000000
VERSION     DBMDVO010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43924_length_1409_cov_1.005352"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            77..>1409
                     /gene="dnaG"
                     /locus_tag="ACZPE2_06125"
     CDS             77..>1409
                     /gene="dnaG"
                     /locus_tag="ACZPE2_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1319461.1"
                     /translation="MALSDEFLDDLRRRADIESTVSSYVSLKRKGKILTGLCPFHNEK
                     TPSFTVYPETQSYYCFGCGNGGDVITFIRNIENLDYMEAVKLLADRHGVSMPQDGYDS
                     GLSKKRTEMYGANREAARFFHAKLYSPEGRQGLEYFYSRGLTDDTIRRFGLGYAPDSW
                     HDLENYLVSKGYSQQLLYEANILRSTVKNGKRYYYDAFKNRAMFPVIDLRGNVIAFSG
                     RRIHDADSDRKYVNTSDTLVYKKGSNLFALNFAKKSKSDSIILCEGNLDVISLHQAGF
                     TNAVAGLGTALTEEQAHLLSHYAGEIFICYDSDEAGQKATRKAINILGKTTLKVRIIH
                     MTGGKDPDEIIQKFGAEGFKRCLDSAANDVEFSLLSERSKYDISSPAGRSDYLKAAAA
                     VLASLNSPVELDIYISRLSEEFSVNKDAVAAQVKIERAKRAKKQKNEFYREV"
BASE COUNT          396 a          291 c          341 g          381 t
ORIGIN      
        1 tcttcacgtg tataataatt tgtttattcc taagtcctgg attgggtggg acaatctttg
       61 agaggggtga tgttatatgg ctttaagcga tgaatttctt gatgatcttc gccgccgcgc
      121 cgatattgaa tctaccgttt cctcgtatgt ttcgcttaaa cgaaagggca aaatattgac
      181 gggactttgt ccgtttcaca acgaaaagac accgtcgttt accgtatatc ccgaaacgca
      241 gtcgtattat tgcttcgggt gcggcaacgg cggagacgtt ataaccttta tacgaaatat
      301 tgaaaatctc gattatatgg aggctgttaa gcttcttgcc gacagacacg gtgtttccat
      361 gccgcaggac ggatatgatt cgggactcag caaaaaaagg accgagatgt acggtgccaa
      421 ccgtgaggcg gcaagatttt tccatgcgaa gctctattcg ccggagggca ggcagggcct
      481 tgaatacttc tattcgcgcg gacttaccga tgatacaata cgtcgtttcg ggctcggata
      541 cgctcctgat tcttggcacg atcttgaaaa ttatcttgtg tcaaagggtt acagtcagca
      601 gcttctatat gaggcgaata ttctgaggtc aacggtaaaa aacggaaaaa gatattatta
      661 tgacgccttt aaaaacaggg ctatgtttcc ggttattgat ttgcgcggca atgttattgc
      721 cttcagcgga agacgtattc acgatgcgga cagcgaccgt aagtatgtaa atacaagcga
      781 cacgcttgtt tataaaaagg gcagcaatct ttttgccctt aattttgcaa aaaagtccaa
      841 gagcgattcg ataattctgt gcgagggtaa tcttgatgtt atttcactgc accaagcagg
      901 ttttacaaat gccgtagcgg gtctcggcac agctcttacg gaagagcagg cacatctgct
      961 ttcccattat gcgggcgaaa tttttatctg ttacgattcc gatgaggcag gacaaaaggc
     1021 aacaagaaaa gcgataaaca tactcggcaa gacaactctc aaggttcgta taatacatat
     1081 gaccggcggc aaggaccccg acgaaataat acaaaaattc ggtgccgaag gcttcaagcg
     1141 ttgccttgat tctgcagcga acgacgttga gttttcgctt ctttccgaac gttcaaaata
     1201 tgatatatcg tctcctgccg gcagaagcga ttatttaaaa gcagcagcag ctgttttggc
     1261 gtcgcttaac agtccggttg agcttgatat atatatttcg aggctttcgg aggaattttc
     1321 ggtgaataaa gacgcggtgg ctgcacaggt taaaatcgaa cgtgcaaaac gcgctaaaaa
     1381 acaaaaaaac gaattttatc gcgaagtgc
//
LOCUS       DBMDVO010000419         1407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43980_length_1407_cov_1.570444, whole genome shotgun sequence.
ACCESSION   DBMDVO010000419 DBMDVO010000000
VERSION     DBMDVO010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43980_length_1407_cov_1.570444"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            203..1069
                     /locus_tag="ACZPE2_06130"
     CDS             203..1069
                     /locus_tag="ACZPE2_06130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1319462.1"
                     /translation="MKSLQDKYILRNGVEVPCIGFGTWKMPDEVAENAVKAAIDTGYR
                     HIDTAGAYRNEKGVGDGIKKSGVKRDEIFLVSKLPNDDHGYEKAIAACESSLLKLGTD
                     YLDGYLTHWPVLVTNWDRLEDDLCDTWRAMMRLRDDGKVRAIGTSNFMAEHVEILKKN
                     LGDYPLINQVQMHPQNPQDEMIAYCKENSVLPEAWSPLIQGQAFKRELLIELAEKYKV
                     SVAQLCIRFIVQKGAVPIPKSANPDRIRNNADVFGFEISDEDMERIASLRVYGRIGDP
                     PSVPRTHQVVGF"
     gene            complement(1126..>1407)
                     /locus_tag="ACZPE2_06135"
     CDS             complement(1126..>1407)
                     /locus_tag="ACZPE2_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1319463.1"
                     /translation="HGFKQFTTTLLASVYQKHELIAAAGERFGKEFGVEFLYRDFRPN
                     FRAGNQRARELGFYMQKYCGCIFSEEDRYKKAILREKERFLEELDSFTL"
BASE COUNT          427 a          312 c          338 g          330 t
ORIGIN      
        1 ggatacacaa tagaggtagg cttaggtcaa agccccctgc cccttacgca gtttgacaaa
       61 atatacagcg acaacgtggg gattcttaaa ggcggcataa ccgaaatatg ataagatata
      121 acgacagcgg cgataaattt aaggtgtaaa atcgccgcat attgtgatac aatatactca
      181 tctttgaaat gaaggtgaga atttgaaaag cttacaggac aaatatatac tcagaaacgg
      241 cgtagaagta ccgtgcatag gcttcggcac atggaaaatg ccggatgaag tcgcagaaaa
      301 tgccgtaaaa gccgcaatag atacaggcta ccgtcatatc gacaccgcag gcgcttacag
      361 gaacgaaaaa ggcgtcggcg acggaataaa aaagagcggc gttaaaaggg acgaaatatt
      421 tttggtaagc aagctgccaa acgacgacca cggctatgaa aaagccatag ctgcgtgcga
      481 gagctcgctt ttaaagctcg gtacggatta cctcgacggc tatttaacac attggccggt
      541 gcttgtcaca aactgggacc gcctcgaaga cgacctttgc gatacctggc gcgcaatgat
      601 gagactgcgc gacgacggca aggtaagagc catcggcaca agtaacttta tggcggagca
      661 tgttgagatt ttaaagaaaa atctcggcga ttatccgctg ataaatcagg ttcagatgca
      721 tccgcaaaat ccgcaggacg aaatgatagc atactgcaaa gaaaactctg ttttgcccga
      781 agcctggagt ccgctcatac aaggtcaggc atttaagcgt gaactcctaa tagagcttgc
      841 cgaaaaatac aaggtttcgg tagcccagct ttgcatacgc tttatagtgc aaaagggtgc
      901 cgttcccatt cctaagtcgg caaatcccga cagaattcgg aacaacgccg atgtattcgg
      961 ctttgaaatt tccgatgaag atatggaaag aatagcgtcg ctcagggtct acggcagaat
     1021 aggcgatccg ccgtcggtgc cgagaactca tcaggtagtc ggattttaaa tcacgtataa
     1081 aaaaacggct tatcgggatc aagctcccta taagccgttt tatctttata atgtaaaaga
     1141 atcaagttct tcaagaaacc tttctttttc gcggaggatt gcttttttgt atctgtcctc
     1201 ctcagaaaag atacatccgc agtatttttg catataaaag ccgagctctc ttgccctttg
     1261 attgccggct ctgaaattcg gtctgaaatc gcggtagaga aattccacgc cgaactcttt
     1321 tccgaaacgc tcacctgccg cggctataag ctcgtgcttt tggtaaacgc ttgcgagaag
     1381 cgtggttgta aactgcttaa aaccgtg
//
LOCUS       DBMDVO010000420         1407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_43983_length_1407_cov_1.526034, whole genome shotgun sequence.
ACCESSION   DBMDVO010000420 DBMDVO010000000
VERSION     DBMDVO010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43983_length_1407_cov_1.526034"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1407)
                     /locus_tag="ACZPE2_06140"
     CDS             complement(<1..>1407)
                     /locus_tag="ACZPE2_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319464.1"
                     /translation="NVLFYRTDGQKYSENTLNGATNTGTPVKTYYDQETEIPRAIFNA
                     CQKYIEDPNSYDYRAVKYIEDLIAGYKKSIEEYNKSEEAGKSGIIDASFLEESALDTK
                     AAALGMTEAWEKYKTAKQACIDGKTYGSANSGAEDFIPMISTKLEQLPDFDENTLAVS
                     DEDKAVVKSLYEVYIRLNLSQLAMLGRAEEEKLLKYFSFIEEELADNSVVIGQALTNN
                     KVIPFGDFDSLKKGTRSWQFENSKYAGQVGQDYYYSKGYLTYQVPFASLSAHSQDTAT
                     PTLIPTGALGSDPQRNGITSYRQNGTSAIITSNGLEGKNAATVTINNQYAEDRKGWYN
                     CISITKGLASEEPSSSGDYSKLRANNMSSESLGDFAKSYTKNDIGETDRLALVFYADF
                     SQLDTFSMAFNISSYYDGAAEDYTLDFGSNNAASYFYLINPKTGEWAKCTPKDESKRR
                     IYAYTSEYGIDTGDGDYL"
BASE COUNT          314 a          321 c          341 g          431 t
ORIGIN      
        1 tcaggtaatc gccgtctccc gtatctatgc cgtactccga ggtgtaagcg taaattcttc
       61 tcttgctttc atccttggga gtacatttag cccattcacc cgtcttgggg ttaatgagat
      121 agaagtaaga agccgcgttg ttgctgccga agtcgagggt gtagtcctcg gcggcaccgt
      181 catagtatga gctgatatta aacgccattg agaatgtgtc aagctgagag aagtctgcgt
      241 agaatacaag tgccagacgg tcggtttcgc ctatgtcgtt tttggtatac gacttagcaa
      301 aatcgccgag actctcagac gacatattgt tggctctcag tttggagtaa tcgccgctgc
      361 tgctcggttc ttcgctggca agtccctttg taatgcttat gcagttatac cagccttttc
      421 tgtcttcggc atactggtta tttatggtaa ccgttgcggc gtttttgccc tcaaggccgt
      481 ttgatgtaat gattgccgaa gtaccgtttt gacgatagct tgtaataccg tttctctgcg
      541 ggtcactacc gagagcgccg gtcggtataa gtgtaggcgt tgcggtatcc tgagagtgcg
      601 ccgaaagtga agcaaaggga acctgatatg tcaaatatcc cttagaatag tagtaatcct
      661 gacctacctg gccggcatat tttgagtttt caaattgcca gcttctggtg ccttttttca
      721 aggaatcgaa gtcgccgaac ggtataacct tgttgtttgt aagcgcctgg cctattacaa
      781 cagagttgtc ggcaagctct tcttctatga agctgaagta ctttaagagc ttctcctctt
      841 ccgctctgcc gagcatagcg agctggctta agttaaggcg gatataaacc tcataaaggc
      901 tcttaacaac ggctttatcc tcatcgctta ccgcgagagt attttcgtcg aaatcgggaa
      961 gctgctcaag cttcgttgaa atcatcggaa tgaaatcctc tgcacccgag ttagcagagc
     1021 cgtaggtttt accgtctata caagcctgct tggcggtctt gtatttttcc caagcctcgg
     1081 tcataccgag tgctgccgcc ttggtatcga gcgcagactc ctcaaggaat gaagcatcga
     1141 ttataccgga ttttccggct tcctcggatt tgttatattc ctcaattgat tttttataac
     1201 cggctatcaa gtcctcgata tacttaacgg cgcggtaatc gtaggagttc ggatcttcaa
     1261 tgtacttttg gcaggcattg aaaatagcgc gcggaatttc tgtttcctgg tcataatatg
     1321 tcttaacggg cgtgcctgtg tttgttgctc cgttaagggt attttcgctg tatttctgac
     1381 cgtctgtacg gtagaacaat acattat
//
LOCUS       DBMDVO010000421         1406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44038_length_1406_cov_1.080460, whole genome shotgun sequence.
ACCESSION   DBMDVO010000421 DBMDVO010000000
VERSION     DBMDVO010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44038_length_1406_cov_1.080460"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>359)
                     /locus_tag="ACZPE2_06145"
     CDS             complement(<1..>359)
                     /locus_tag="ACZPE2_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1319465.1"
                     /translation="GLTYSLKANKGESFYTYNPSYRWFDCTDSGKNNLMIKAYAVSDT
                     ECTEHTYGEWIIDRDSTCIATGEKHRVCSKCNHIETATIEKKPHNYITSVVAPTYTTD
                     GYTIHKCSVCNSSYIDN"
     assembly_gap    360..369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<370..1364)
                     /locus_tag="ACZPE2_06150"
     CDS             complement(<370..1364)
                     /locus_tag="ACZPE2_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012340.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HIR1319466.1"
                     /translation="MVSHPSLYGKLNPSGDYATYTASSATDYLTLGGNVWAAGLGLMN
                     GIGPYNENSDYPYSEYNTPSIVNKNFTESEYYEVRNSSVAKITGVFQAHINNNSDNDE
                     FKQLIMDYGAASLSYCDTANYGRTDGKFGSDGSSYYYYCPEAYTSNHAVTVVGWDDSI
                     PASAFNTAPAGDGAWLIKNSWGEYSRDNGYFWLSYYDKSISGVGIAYDFTVDGADDYF
                     DTRYSYDGGNSLASFGYSRPDIYGANVFTADKDSYVTGAAAYTSAGNNIELSVYTGLK
                     DASNPTSGTKSAVATMSNVKYEGYYSLKFDAPVKIKKGESFAIVAKITKDSGTVR"
BASE COUNT          352 a          318 c          298 g          428 t
ORIGIN      
        1 aattatcaat ataggagctg ttgcaaaccg aacacttatg tatcgtatat ccgtcagttg
       61 tatatgtcgg tgcaaccact gaagtaatat aattgtgagg tttcttttcg attgttgccg
      121 tttcaatatg attgcacttt gagcatactc tgtgcttttc gcctgttgcg atacaggttg
      181 aatccctgtc gattatccat tcgccgtatg tgtgttctgt gcattcggta tcggaaacgg
      241 cataagcctt tatcatcaga ttattttttc cgctgtcggt gcaatcaaac catctgtacg
      301 aaggattata tgtatagaag ctctcgcctt tatttgcttt gagtgagtat gtaaggccgn
      361 nnnnnnnnna ttctgaccgt gccggaatcc ttggttattt ttgctactat cgcaaaagat
      421 tcacccttct tgatctttac tggagcatcg aacttcagcg aataataacc ctcgtacttg
      481 acgttgctca ttgtggcaac ggcggatttt gtgcctgatg tgggattgct tgcatccttt
      541 aaacctgtat acacactcag ctcgatgtta tttcctgcgg aagtataggc tgcagctccc
      601 gtaacatatg aatctttatc tgcggtgaat acattcgcac cgtatatatc aggtctggaa
      661 tagccaaatg aggctaagct gtttccgccg tcatagctgt aacgggtatc aaagtaatca
      721 tcggcgccat cgaccgtaaa gtcataggca atgccgacac cgcttatcga cttatcatag
      781 tatgacagcc agaaatatcc gttatctctt gaatattctc cccagctgtt cttgatgagc
      841 catgcaccgt cacctgcagg ggcagtgtta aatgcagacg caggtatact gtcgtcccag
      901 cctactaccg tgactgcatg attagatgta tatgcctcag ggcaatagta gtaataactg
      961 ctgccgtcac tgccaaattt tccgtcagta cgtccgtaat ttgcagtatc gcaatagctg
     1021 agcgaagccg caccgtaatc cataataagc tgtttaaact catcgttgtc gctgttgttg
     1081 ttgatatgag cctggaacac tcccgtgatt tttgcaacag aagaatttct tacctcataa
     1141 tactcagatt cggtaaaatt cttattaacg atactcggcg tattgtactc gctgtaagga
     1201 taatcactgt tttcattgta aggtcctatg ccattcatca ggccaagacc cgcagcccat
     1261 acattaccac cgagtgttaa atagtcggta gccgatgaag ctgtgtatgt cgcataatcg
     1321 cccgacgggt tgagtttacc gtaaagcgac ggatgactca ccatatacgc aagcagattt
     1381 tccgaaaggt ctgtatttcc ggaatc
//
LOCUS       DBMDVO010000422         1405 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44060_length_1405_cov_1.499233, whole genome shotgun sequence.
ACCESSION   DBMDVO010000422 DBMDVO010000000
VERSION     DBMDVO010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44060_length_1405_cov_1.499233"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="ACZPE2_06155"
     CDS             <1..547
                     /locus_tag="ACZPE2_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1319467.1"
                     /translation="KYYQVSGALEMTAQTSAEGAVQQYSYSATFPLLLNISGEPTYFM
                     ALKDSSELVKMYAMVNVKQSTIVGTGYNLTECTENYAAELKRNGVNVDIDVDEMGAKD
                     DPTATAPETEEISGKITEIRSVVTGGETYFYLKLDAGSTFYKVPVALAEKVVILNVGD
                     SVTVLVPKESSGDIVEVSSLK"
     gene            629..>1405
                     /gene="mtnA"
                     /locus_tag="ACZPE2_06160"
     CDS             629..>1405
                     /gene="mtnA"
                     /locus_tag="ACZPE2_06160"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR1319468.1"
                     /translation="MFDMHGETVCYNEKDETAVIIDQTLLPGEIVTLELWEKEEMYDA
                     IKRLAVRGAPAIGVFAAIGLSVLAARDKTADEKTLIEHFLENLEYLTSSRPTAVNLFA
                     AADEMKRTALLHKGEGADAVRAALREKALELHGKDIEVCRKIGEYGARLLKNCDSVLT
                     HCNAGRLATVKYGTALAPVYVAAENGHKIRVFSDETRPLLQGARLTSFELANAGIDTT
                     LICDNAASYVMKNGLVSAVLVGCDRVAANGDTANKIGTSGV"
BASE COUNT          379 a          337 c          390 g          299 t
ORIGIN      
        1 aaagtattat caggtgtcag gtgcgcttga aatgaccgct cagacctccg ccgagggcgc
       61 ggttcagcag tattcgtatt cggctacctt cccgcttctg ctcaacatca gcggcgagcc
      121 gacatacttt atggctctca aggattcaag cgagcttgta aagatgtacg ccatggttaa
      181 cgtgaagcag tccacgatag tcggtacggg atacaatctc accgaatgta ccgaaaacta
      241 cgcggcagag cttaaacgca acggcgtaaa cgtggatatt gacgttgacg aaatgggcgc
      301 aaaggacgac ccgaccgcga ccgcccccga aacagaggaa ataagcggca agataaccga
      361 gataagaagc gtcgtaacgg gcggcgaaac gtacttctat ttgaagcttg acgcaggcag
      421 tacgttctat aaggtccccg tggcactcgc cgaaaaggtt gtaatcttga acgtcggcga
      481 cagcgttacc gtcttagtac ccaaggagtc ctccggtgac atagttgagg tatcctcact
      541 caaataacac ctttaaaacc gccgcgcgga aatttcggca gctccgaaaa aacgcgcggc
      601 tttaattttt accgaacgga gtaaaattat gtttgatatg cacggcgaaa ccgtatgcta
      661 taacgaaaaa gacgagaccg cagtgattat agaccagact ttgctgcccg gtgaaattgt
      721 cacgcttgag ctttgggaaa aagaggagat gtatgacgca ataaagcgtc ttgccgttcg
      781 cggcgcaccg gcaataggtg tgtttgcggc aatagggctg tcggttttgg ccgcacgcga
      841 caaaacagca gacgaaaaaa cgcttattga gcattttctc gaaaatcttg aatatctcac
      901 ctcctcgcgc cctaccgcgg taaatctttt tgcggcggcg gacgagatga aaaggacggc
      961 tcttttacat aagggcgagg gcgcggacgc cgtaagggcg gcactcaggg aaaaggcttt
     1021 ggagctacat ggcaaagata ttgaggtgtg ccgtaaaatc ggtgaatacg gagctcggct
     1081 tctcaagaac tgcgacagtg ttttgaccca ctgcaatgcg ggcaggcttg cgacggtaaa
     1141 gtacggaacc gctctggcac ccgtatatgt ggcggcggaa aacgggcata aaatacgcgt
     1201 gttctctgat gagacaagac ctcttttgca gggcgcgcgc cttacgagct ttgagcttgc
     1261 aaacgcaggt atagatacta cgctgatatg cgataacgcc gcatcgtacg ttatgaagaa
     1321 cgggcttgtt tccgcggtgc ttgtcggatg cgacagagtg gcggcaaacg gcgatacagc
     1381 caataaaata ggcacctccg gagtt
//
LOCUS       DBMDVO010000423         1405 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44063_length_1405_cov_1.421012, whole genome shotgun sequence.
ACCESSION   DBMDVO010000423 DBMDVO010000000
VERSION     DBMDVO010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44063_length_1405_cov_1.421012"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1405)
                     /locus_tag="ACZPE2_06165"
     CDS             complement(<1..>1405)
                     /locus_tag="ACZPE2_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1319469.1"
                     /translation="GQIIRKESDNGTDCCIPAESGADNRTDNIGQRERENPLPSPAGG
                     GIRRVSTKQEEQLNSYENQVEHYTERINSENGWTLEGIYADKGISGTSVKNRDEFNRM
                     IRRCKQGKIDMIITKSIARFARNTVDCLKYVRLLNDLGVDVYFEEQGIHSNQPGAEFY
                     ITIYGSIAQSESENISANIKWGKAQAAREGKVVFRYKNFLGYRKGEDGQPEIVPEEAE
                     TIRFIYDRFLAGDSLKGIAKLLEEKGIKSPTGKAEWQFSTIQSILSNERYKGDAIINK
                     TYITDCISKKVRVNNGERPKYYVENSHPAIIDSATFGRVQEELARRSGKRKISRKAKT
                     EQGKYSSKYALTELLVCGECKSAYRRCTWTASGKKKIVWRCINRIEYAKKYCHNSPSV
                     EESILQRAVMAAIMETANQNAEILRTLKLHIGIGLAGEKSEDNSIDLQIRIAEIDAEF
                     KKMLDRVSTDTIEAFDEE"
BASE COUNT          238 a          354 c          363 g          450 t
ORIGIN      
        1 tttcctcatc aaaggcttcg atggtgtcgg tggatacccg gtcgagcatc tttttgaatt
       61 ctgcgtcgat ttcggcgatc cgtatctgta ggtcgatgct gttatcctcg ctcttttctc
      121 ccgccaagcc tatgccgatg tgcagcttca gtgttcgcaa tatctcagca ttttggttgg
      181 cggtttccat gattgccgcc atcacagccc gttgcagtat gctttcctct acggatggcg
      241 agttatgaca gtatttcttg gcatattcaa tgcggttgat gcatcgccat acaattttct
      301 ttttgccgct tgccgtccat gtgcatcggc ggtaggcaga cttgcattca ccgcagacga
      361 gcagttctgt cagagcgtat ttacttgagt atttgccttg ctcggtcttg gcttttcggc
      421 tgattttgcg tttgccggag cgccttgcga gttcctcctg aactctgccg aaggtagccg
      481 agtcgatgat ggcagggtgg ctgttttcca catagtattt cgggcgttcg ccgttgttga
      541 ctctgacctt tttgctgatg cagtcggtga tgtaggtctt attgatgatg gcatcgcctt
      601 tgtatctctc gttggagaga atggactgta tcgtggaaaa ctgccattct gcttttcccg
      661 tcgggctttt gatgcccttt tcttcaagta gtttggcaat tccttttaag ctgtcgcctg
      721 cgaggaagcg gtcatatata aagcggatcg tttcggcttc ttcggggacg atttcgggtt
      781 gtccgtcctc gcccttgcga tagccgagga agttcttgta tctgaatact accttgcctt
      841 cacgggcggc ctgtgccttg ccccatttaa tgttggcgct gatattttcc gattcgcttt
      901 gggcgatgct gccgtagatg gtaatgtaaa actcggcacc gggctgattg ctgtggatgc
      961 cctgttcctc gaagtacaca tcgacaccga ggtcattgag cagtcggaca tacttcaagc
     1021 agtccaccgt gttccgcgcg aaccgggcaa tggatttcgt gatgatcatg tcgattttcc
     1081 cttgcttgca gcggcggatc atacggttga attcatctcg gtttttcacg cttgtgccgg
     1141 agatgccctt gtcggcgtag ataccttcaa gcgtccatcc gttttcggaa ttgatgcgtt
     1201 cggtatagtg ttccacctga ttttcataac tgttaagctg ttcctcctgc ttagtggaaa
     1261 cgcggcgtat gccgccaccc gcaggactcg gtaatgggtt ttcacgctct ctctgaccga
     1321 tattgtcggt tcgattatcc gcaccactct cggcggggat acaacagtct gttccattgt
     1381 ctgactcctt tcgtatgatc tgtcc
//
LOCUS       DBMDVO010000424         1404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44122_length_1404_cov_1.149655, whole genome shotgun sequence.
ACCESSION   DBMDVO010000424 DBMDVO010000000
VERSION     DBMDVO010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44122_length_1404_cov_1.149655"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..266)
                     /locus_tag="ACZPE2_06170"
     CDS             complement(15..266)
                     /locus_tag="ACZPE2_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1319470.1"
                     /translation="MNLMSYIVERALILIPALNVLGLIFKSIERIPDKFIPLLLLIFG
                     ISGSIALTGLSADSVIQGVLVTGAAVYGNQVVKQLKKDN"
     gene            complement(276..542)
                     /locus_tag="ACZPE2_06175"
     CDS             complement(276..542)
                     /locus_tag="ACZPE2_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319471.1"
                     /translation="MSENELFEHRIKTLENDVKILYGKVNTFAINQAEENTKLDNLLL
                     SVGELKESISEIKRRPSAFWDKLLFALIGAVGTALGYALIGFVN"
     gene            complement(552..>1404)
                     /locus_tag="ACZPE2_06180"
     CDS             complement(552..>1404)
                     /locus_tag="ACZPE2_06180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1319472.1"
                     /translation="SKWQGDINWEKVRASGVEFAMLRAGFGRCQGQNDTSFERNYREA
                     KKNGILVGAYHYSYAKTVSDAEKEAEYFLSLISGKSFEFPVAFDIEDNSQKNLSREEI
                     SNIVEAFCGRTEKAGYFVSVYANLWWLNNKISDRVKERYDIWLAQWADAPSYGGKYGM
                     WQYTSSGKTDGITGNTDMDLAFKDYPNIMRANGLNGFSKGAAESTDNVKSGTFPPRRS
                     VALCNTPLFSSAYSKAPSARKSGTYYIYDGIEINGRYRITSSASFALKKPIGKNVTGF
                     VNADDIR"
BASE COUNT          344 a          360 c          290 g          410 t
ORIGIN      
        1 gcccctttta aaaatcaatt atcctttttt aactgcttta ccacctgatt gccgtaaacg
       61 gcggctcccg taacaagcac tccctgaatt accgaatcgg cagacagccc cgtcagagct
      121 atgcttccgg aaattccgaa aatcaacagc aaaagcggta tgaatttatc gggtattctt
      181 tcaatagatt taaaaatcag tcccaaaacg tttaatgccg ggattaaaat cagcgcgcgt
      241 tcgactatat atgacatcaa attcatattt cctccttaat ttacaaagcc gataagcgca
      301 tagcctaacg cagttccgac ggcaccgata agcgcaaata agagcttatc ccaaaacgcc
      361 gatggtctgc gtttaatttc gcttatgctc tccttcagct ctccgacgga caagagcaag
      421 ttatcaagct ttgtattttc ctctgcctga tttatcgcaa aggtatttac ctttccgtac
      481 agtatcttaa cgtcgttttc aagtgttttg attcgatgct caaaaagctc gttttcactc
      541 atagcggtgc ctcaccttat atcatcggcg tttacaaatc ccgtgacatt tttacctatc
      601 ggctttttaa gagcaaagct cgcagaggag gttatgcggt acctgccgtt tatctcaatc
      661 ccgtcgtaga tatagtaagt acccgatttt ctcgccgacg gagcttttga atacgcagac
      721 gaaaacaagg gtgtattgca aagtgccacg gaccggcgcg gtggaaatgt gccgcttttt
      781 acgttatcgg tgctttctgc ggcacctttt gaaaagccgt tgagtccgtt ggctctcata
      841 atattcgggt agtccttaaa ggctaagtcc atatcggtat tgcctgttat gccgtccgtt
      901 ttgcccgagg acgtatactg ccacataccg tattttccgc cgtaggaggg tgcgtcggcc
      961 cattgcgcga gccagatatc atagcgctcc tttaccctgt cggaaatttt gttattgagc
     1021 caccacagat tcgcatagac cgaaacaaaa tatcctgcct tttccgttct gccgcaaaac
     1081 gcctcgacaa tattgcttat ttcctcacgt gataggtttt tctgcgagtt gtcctcaata
     1141 tcgaatgcca cgggaaattc aaagcttttt ccggatataa gcgagagaaa atactccgcc
     1201 tccttttctg cgtccgagac ggtttttgcg tagctgtaat ggtatgcgcc gacaagtatg
     1261 ccgtttttct ttgcctcgcg gtaatttctt tcaaatgaag tgtcattttg gccttggcac
     1321 ctgccgaagc ccgcgcgcag catggcaaat tcgacacccg aagcgcgaac cttttcccaa
     1381 tttatatcgc cctgccactt tgac
//
LOCUS       DBMDVO010000425         1398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44348_length_1398_cov_1.239013, whole genome shotgun sequence.
ACCESSION   DBMDVO010000425 DBMDVO010000000
VERSION     DBMDVO010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44348_length_1398_cov_1.239013"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="ACZPE2_06185"
     CDS             <1..315
                     /locus_tag="ACZPE2_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319473.1"
                     /translation="VHKGLASKDFTKFSSIVEQKEYCTKTGLLAGSGCSSRATGWFSK
                     SNMPGYCTSCSGGGSKDGKIEGETKKTTDGSTKPSETTKAAAETTKAPEATKAAEATK
                     AQ"
     gene            330..>1398
                     /locus_tag="ACZPE2_06190"
     CDS             330..>1398
                     /locus_tag="ACZPE2_06190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1319474.1"
                     /translation="MGNLLFKNAHVIDPSQKLDTVGDVLVVDGKIVDFGKALSCSADN
                     IIDASGLVLAPGLIDLHVHLRDPGLEYKEDIISGCRAAAAGGVTTVCAMPNTKPVCDN
                     IETVKYILEKAANADAHVLPIGAVSVGENGEKLTDMQALKEAGCCAFSDDGVPVKTAE
                     LMREALKEAARLDMPVFAHCEEKTISRDGIVNECDVAKDLNMPQIPVSAEDVGTARET
                     ALLMSAPKNARLHICHVSTGNSALLIASAKRNGFNVTAETCPHYFIFNDEKLLLKDAD
                     YRMNPPLRSAVDQFSVLYSLIDGVIDVIATDHAPHSPEEKADFYKAPNGVIGMETSFS
                     ASYTALVASGLISLYELIDKMA"
BASE COUNT          386 a          311 c          349 g          352 t
ORIGIN      
        1 gttcataaag gactcgcttc taaagatttt acaaaattct catctattgt tgagcaaaaa
       61 gagtattgta caaagacggg cttgcttgcg ggcagcggat gttcgagtcg cgcaaccggc
      121 tggttctcaa agagcaatat gccgggctat tgtacatctt gctccggcgg cggaagtaag
      181 gatggcaaga tcgagggaga gaccaaaaag acaaccgacg gcagcactaa gccgtcagaa
      241 acaacaaagg cggctgctga gacgacaaaa gctcccgagg ccacaaaagc cgcagaggct
      301 accaaagctc aataatcaaa ggagttttta tgggcaattt gctgtttaaa aatgcacacg
      361 ttatagaccc ttcacaaaag cttgatactg tcggcgacgt tcttgtcgtc gacggtaaga
      421 ttgtcgattt tggcaaggct ttaagctgct ctgccgataa tatcattgat gcttcggggc
      481 ttgttttggc accggggctt attgatttac acgtgcattt gcgtgacccg ggtcttgaat
      541 ataaagagga tataatttcc ggatgccgtg cggcggcggc gggcggcgtg acaacggttt
      601 gcgccatgcc taatacaaaa ccggtatgcg ataacataga aacggtaaaa tatatacttg
      661 aaaaagcggc taatgccgat gctcacgttc tcccaatagg tgccgtttcc gtcggcgaaa
      721 acggtgaaaa gctcaccgat atgcaggctt taaaagaagc cggttgctgc gctttttcgg
      781 atgacggcgt tccggttaaa accgcagagc ttatgcgtga agcgctcaaa gaggcagcaa
      841 gactcgatat gcctgttttt gcccattgcg aagaaaaaac aatttcacgc gacggcattg
      901 tgaatgaatg cgatgttgca aaagacctta atatgcctca gataccggtt tcggcagaag
      961 acgtgggaac tgcgcgcgag acagcccttt taatgtcagc ccccaaaaat gcacggttgc
     1021 atatttgtca cgtcagtacg ggcaattcag cacttcttat agcctccgca aagagaaacg
     1081 gatttaatgt aactgctgaa acttgtccgc attattttat atttaacgat gaaaagcttt
     1141 tgttaaagga tgcggattat cgaatgaatc cgccgcttcg cagtgccgtg gaccagtttt
     1201 ccgttctcta ctcgcttatt gacggcgtaa tagatgtaat tgcaaccgac cacgcacctc
     1261 attcaccgga agaaaaggca gatttttaca aagctcccaa cggcgttata ggtatggaga
     1321 catctttttc cgcttcatat accgcgcttg ttgcgtcggg acttatcagt ctttatgagc
     1381 ttattgataa aatggcgt
//
LOCUS       DBMDVO010000426         1396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44429_length_1396_cov_1.410811, whole genome shotgun sequence.
ACCESSION   DBMDVO010000426 DBMDVO010000000
VERSION     DBMDVO010000426.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44429_length_1396_cov_1.410811"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..520
                     /gene="purE"
                     /locus_tag="ACZPE2_06195"
     CDS             29..520
                     /gene="purE"
                     /locus_tag="ACZPE2_06195"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1319475.1"
                     /translation="MKKVAIIMGSDSDLPTAEKAATELKKLEIPFEVHVMSAHRTPDE
                     ASAFSKNAEQNGFGVIISIAGMAAHLGGVLAANTVLPVIGVPVGSSFGGLDALLATVQ
                     MPSGIPVATVALGGGKNSAILAAQMLALSDDSLREKLRGMRKEMAEAVREKDDIISNQ
                     FKN"
     gene            543..1256
                     /gene="purC"
                     /locus_tag="ACZPE2_06200"
     CDS             543..1256
                     /gene="purC"
                     /locus_tag="ACZPE2_06200"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1319476.1"
                     /translation="MKKLEQLYEGKAKKVFATDEDGVVIVDYKDDATAFNGEKKGTIM
                     GKGVVNNKMSNYVFGLLEKDGVPTHFIKELSDRETAVKKVSIVPLEVIVRNVAAGSFS
                     KRMGVPEGKELLCPILEFSYKNDELGDPFINDDYALALGLATQDEIDTIKNYTRKVNE
                     CLKKYFLSAGMKLIDFKIEFGRLPDGTIILADEISPDTCRLWDVKTNEKLDKDRFRRD
                     LGNVEEAYNEVFRRLGLKD"
BASE COUNT          418 a          321 c          341 g          316 t
ORIGIN      
        1 gctttatatt ttaccggagg aacaaaagat gaaaaaagtt gcaataatca tgggctctga
       61 cagtgatctg cccacagccg aaaaagcggc aaccgagctt aaaaagctcg aaataccctt
      121 tgaggttcac gtgatgtcgg cacaccgcac accggacgag gcgtcggcct tttcaaagaa
      181 cgccgagcaa aacggattcg gagttataat ttcaatagcc ggtatggcgg ctcatctcgg
      241 cggagtgctt gccgccaaca ccgttttacc ggtaataggc gttccggtgg gttcgtcatt
      301 cggcggactc gacgcacttt tggccacggt acagatgcca tccggcattc ccgtagccac
      361 ggtggctttg ggcggaggca aaaactccgc aattctcgcg gctcaaatgc ttgctctctc
      421 cgacgactct cttcgggaga agctgcgcgg tatgagaaaa gaaatggccg aagcggtccg
      481 tgaaaaagat gatataattt caaatcaatt taaaaactaa aacagaagga gatatacaaa
      541 ttatgaaaaa gcttgaacag ctctatgagg gtaaggcaaa aaaggttttt gcaactgatg
      601 aggacggcgt tgtaatagtt gattataaag acgacgcaac cgcttttaac ggcgagaaaa
      661 aaggcaccat aatgggtaag ggcgtagtta acaacaaaat gtcaaactat gtattcggtc
      721 tgctcgaaaa ggacggagtt cccactcatt ttataaaaga gctcagcgac cgcgaaaccg
      781 ccgttaaaaa ggtttcaata gttccgcttg aggtaatagt aagaaatgtg gccgcaggca
      841 gcttttcaaa gagaatgggc gttcccgagg gaaaagagct cctctgcccc attcttgaat
      901 tcagctacaa aaacgatgag ctcggcgatc cgtttatcaa cgacgattat gcgctcgcac
      961 tcggacttgc gactcaggac gaaatcgaca caataaagaa ttacacaaga aaagtaaacg
     1021 agtgcctcaa aaagtatttc ctctctgccg gaatgaagct tattgacttt aaaatcgaat
     1081 tcggcagact tcccgacggc acaataatcc ttgccgacga aatatcgccc gacacttgcc
     1141 gtctctggga tgtcaagaca aacgagaagc tcgataagga ccgcttccgc cgcgatctcg
     1201 gcaatgtgga agaagcttac aacgaagtat tccgtcgtct cggccttaaa gactaagcag
     1261 tttacaaaag caatttacga ggtattatat gtttgacagt attcatgagg agtgcggcgt
     1321 tttcggtata tactctgaag agaccgcaga cgtggcgtcc gcggcatatt atgcgctctt
     1381 tgcacttcaa caccgc
//
LOCUS       DBMDVO010000427         1396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44434_length_1396_cov_1.315058, whole genome shotgun sequence.
ACCESSION   DBMDVO010000427 DBMDVO010000000
VERSION     DBMDVO010000427.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44434_length_1396_cov_1.315058"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="ACZPE2_06205"
     CDS             <1..1230
                     /locus_tag="ACZPE2_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1319477.1"
                     /translation="ETGNKEYWAKWEINQYTITVKPENGKADITITQDYGTAITAPAD
                     PTREGYTFTGWDTEIPKTMPAENITLKAKWKDIEKPTGEIIIGTNKWHEFLNKLTFGL
                     FFKDTQEVTINAFDNSGTVFVSYLVTNRDLSETELGSLVYNAYDEPFLIEPNGEYIVY
                     AMLVDESMNITYLRSDRITLDNIRPVIGGIENGKTYCEAQTVTIDEKYIDTVTVNGTK
                     VTLDENGGFTLSPADGEQKIIVTDKAGNTAEMTVTVHGGHTFGEWTSNGDGTHSRECT
                     VDGCKGVETMACSGGKATCAEKAVCEYCGKAYGKLDSNNHTDLKHIDAKAATEDTEGN
                     IEYWYCGGCDKYYSDKDGINEIKKADTVTAKLPGDPKFPRTGNASDLALWISLLLVSG
                     GVTGVTAAFRKKKKHKV"
BASE COUNT          418 a          324 c          342 g          312 t
ORIGIN      
        1 gaaacgggca ataaggaata ttgggcgaag tgggagatca atcaatatac catcacagtc
       61 aaacccgaaa acggcaaagc ggatattacc ataacgcagg actacggcac ggcaattacc
      121 gcacctgccg acccgacaag agagggctac accttcacgg ggtgggacac ggaaattccc
      181 aaaaccatgc ctgccgagaa tataacgctt aaagcgaaat ggaaagacat tgaaaaaccc
      241 acgggcgaaa ttatcatagg cacaaataaa tggcacgaat tcttaaacaa gctgaccttc
      301 ggtctgttct ttaaggacac gcaggaggta acgataaacg ctttcgataa cagcggaacc
      361 gtgtttgtct cttatttggt gacaaatcgg gacctctctg agacggaact cggctctctt
      421 gtatacaatg cgtatgatga gccgttttta atagagccta acggagaata tattgtttat
      481 gcaatgcttg ttgacgagag catgaatata acatatcttc gctccgaccg cataacgctt
      541 gataatatcc ggcctgtaat cggcggcatt gaaaacggca agacctactg cgaggcacag
      601 acagttacaa ttgacgagaa gtatatagat acagttaccg taaacggcac aaaggtcacg
      661 cttgatgaaa acggcggctt tactctctct cctgcggacg gcgaacagaa aatcattgtc
      721 accgataagg caggcaacac cgccgagatg accgtgacag tccatggtgg gcacaccttc
      781 ggcgaatgga catcaaacgg cgacggcacg catagccgtg aatgtaccgt ggacggctgc
      841 aagggtgttg aaacaatggc ttgctcgggc ggcaaggcaa cctgcgccga aaaagcggta
      901 tgcgaatact gcggcaaggc ttacggaaag cttgattcaa acaaccacac cgacttaaag
      961 cacatcgatg caaaggcggc aaccgaggat accgagggca atatcgagta ttggtactgc
     1021 ggcggctgtg ataagtatta cagcgacaaa gacggtataa acgaaattaa aaaagccgac
     1081 accgtaacgg ctaagctgcc gggcgatccg aagtttcccc gaacaggcaa cgcaagcgac
     1141 cttgctttat ggatttccct gctgcttgtc agcggcggcg taacaggcgt cacagcagcc
     1201 ttccgcaaaa agaaaaaaca caaagtataa tttttttcgt ttcccttttc tgcttttacg
     1261 gcagtaaaaa gcctccgttt tttaacggag gctttcgttt attgcggctt atgcatattc
     1321 tcgatttttt taatgtattc ggtgctgttc tcaagaaatt tccgagtatc aaaggactcg
     1381 tttttaaggc tgtttt
//
LOCUS       DBMDVO010000428         1395 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44490_length_1395_cov_1.112056, whole genome shotgun sequence.
ACCESSION   DBMDVO010000428 DBMDVO010000000
VERSION     DBMDVO010000428.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44490_length_1395_cov_1.112056"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="ACZPE2_06210"
     CDS             complement(<1..292)
                     /locus_tag="ACZPE2_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1319478.1"
                     /translation="MIEFKNVSKTYDNGTEALKDVNIKINDGEFVFVVGSSGAGKSTF
                     LKIMMREQVPNSGSVIINGYHLNNLKKKEIPLFRRTLGIVFQDFRLIPTMTVF"
     gene            complement(285..539)
                     /locus_tag="ACZPE2_06215"
     CDS             complement(285..539)
                     /locus_tag="ACZPE2_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319479.1"
                     /translation="MGKMHNVTSVILSKVREYNLPYYKMVTNLTFYAIIKDGIMSDYT
                     HFAAYIFYSGCVESLPVGIKLYGRVQRWEYCEKQRYVLSD"
     gene            complement(570..>1395)
                     /locus_tag="ACZPE2_06220"
     CDS             complement(570..>1395)
                     /locus_tag="ACZPE2_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1319480.1"
                     /translation="KYLSMLAVTLSSIKQYYDEKYDRANFIKNVILDNILPGDIYLKA
                     RELHFNNDVSRVVILIRITEHIDISAYDIIQNLFPDKTKDFVINLNETDIALIKEVKP
                     NIDTKDLEKLARSIADGLGTEFYSHVLVGIGTTVDGIKDLARSFREAQVSLEVGKVFD
                     TEKSIVSYDNLGIARLIYQLPTTLCDMFLKEVFKRGSIDNLDQETLFTIQKFFENNLN
                     VSETSRKLFVHRNTLVYRLEKIKKLTGLDLREFDDAIVFKVALMVKKYLDSSPVKY"
BASE COUNT          400 a          292 c          273 g          430 t
ORIGIN      
        1 caaacaccgt catggtcggg ataagtctga aatcctggaa tactataccg agagtccttc
       61 tgaaaagagg aatctctttt ttctttaaat tgttaagatg ataaccgttt atgataacag
      121 aaccgctgtt aggcacctgc tcgcgcatca ttatcttgag aaacgtactc ttgccggctc
      181 ccgatgagcc gaccacaaag acaaattctc cgtcgtttat cttgatattg acatctttaa
      241 gtgcttcggt cccgttatcg taagtttttg atacattttt aaattcaatc acttagaaca
      301 tacctctgct tttcacaata ttcccaccgc tgaactctgc cgtaaagttt aataccgacg
      361 ggcagagact ctacacaacc gctataaaaa atataggcgg caaaatgagt ataatcactc
      421 ataataccgt ctttaattat agcatagaac gtcaagtttg taaccatttt gtaatatggt
      481 aaattatatt cgcgcacttt agacaaaatc acgcttgtaa cattgtgcat ttttcccatt
      541 cgcaacagcg cgcggaaaat tacttttctt taatacttga cggggcttga atcaagatat
      601 ttcttaacca taagagctac tttaaatact attgcgtcgt cgaactcgcg aaggtcaagg
      661 cccgtgagct ttttaatctt ttcaagacga tacacaagag tatttctgtg tacaaagagc
      721 tttcttgagg tttcggatac gttgaggttg ttttcaaaga acttctgaat tgtaaagagc
      781 gtttcctgat cgagattgtc gatagagccg cgcttgaaaa cctcctttaa gaacatatcg
      841 caaagagttg tgggaagctg atatataagt ctcgcaatac cgagattgtc atagctcaca
      901 atgctctttt cggtgtcgaa caccttgccg acctcaagag aaacctgagc ctctctgaat
      961 gagcgtgcaa gatcctttat tccatcaacc gtagtaccta tgccgacaag aacgtgagaa
     1021 tagaactccg ttccgagacc gtcggctatc gagcgtgcaa gcttttcgag atcctttgtg
     1081 tcgatattcg gcttaacctc tttgataagg gcaatatctg tctcgttaag gtttattaca
     1141 aaatccttgg tcttatccgg gaacaggttc tgaattatat cataagcgga aatatctata
     1201 tgctcggtaa ttcttatgag aattacgacg cgtgacacgt cattgttaaa atgaagctct
     1261 ctcgccttga ggtaaatatc accgggcagt atgttatcaa gaattacgtt cttgataaag
     1321 tttgcgcggt cgtatttttc atcataatac tgcttaatgc ttgagagtgt tacggcaagc
     1381 attgagagat atttc
//
LOCUS       DBMDVO010000429         1394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44521_length_1394_cov_1.465584, whole genome shotgun sequence.
ACCESSION   DBMDVO010000429 DBMDVO010000000
VERSION     DBMDVO010000429.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44521_length_1394_cov_1.465584"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="ACZPE2_06225"
     CDS             complement(<1..778)
                     /locus_tag="ACZPE2_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1319481.1"
                     /translation="MQNFVELIQNVNSKVNSVVWGPPMLILMLLTGLYLTCGTRFFQI
                     RRFCYTIKNTVAAIFKDRKVTKTKDEKAISQFQALATALAATVGTGNIVGVATAISSG
                     GAGAVFWMWISALLGMMTSFSENALGIYFRRKNEKGEWVGGPMYYIEKGLGQKWLAVI
                     FSVFCLFASFGIGSVAQVNGISTALKGSFNIPGVVTGIVVCALIAVIIYGGLKRIVTV
                     TEKFVPFMAAFYIIGALIIIIVNFRHIGDAFGEIFSGAFSM"
     gene            complement(863..>1394)
                     /locus_tag="ACZPE2_06230"
     CDS             complement(863..>1394)
                     /locus_tag="ACZPE2_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1319482.1"
                     /translation="TELAEAKKQFDVNFFGCFICSKNVIKYMRLQGGGRIVNLSSLAA
                     PLAIPFQAFYSASKAAVNSLTLALANEVRPFNIKVCAVMPGDVKTGFTAAREKNTAGD
                     EFYGETLKRAVAGMERDEQNGMSPDDIADAVYAAATKARPKLLSTKGTKYHLFLALSK
                     ALPVSLVNIIVGKLYS"
BASE COUNT          385 a          348 c          295 g          366 t
ORIGIN      
        1 gcatactgaa cgctcccgaa aatatctcgc cgaaagcgtc gcctatatgg cggaagttta
       61 cgattattat tataagcgcg cctattatgt aaaacgccgc cataaaaggc acaaattttt
      121 ccgtcaccgt gactattctt ttcagtccgc cgtaaattat aacggctata agggcgcaaa
      181 cgactatccc cgtgacaacg cccggtatat taaacgaacc cttaagtgcc gtggatattc
      241 cgttgacctg agctaccgag cctattccga acgacgcgaa caggcaaaaa acagaaaata
      301 taaccgcaag ccacttttgg ccgagtcctt tttcgatata atacataggg ccgcccaccc
      361 actcgccttt ttcgtttttt ctgcggaaat atatgccgag cgcattttcg gaaaagctcg
      421 tcatcatacc gagcaaagca gaaatccaca tccaaaatac cgcacccgct ccgccggagg
      481 aaatcgcggt ggctacgcct acaatgttgc ctgtgcctac cgttgcggca agagccgttg
      541 caagtgcctg aaactgcgat atggcttttt cgtccttggt ttttgttacc ttgcggtctt
      601 taaaaattgc ggcaaccgta tttttaattg tgtagcaaaa acggcgtatt tggaaaaatc
      661 gcgtaccgca cgtgagataa agaccggtaa gcagcatcaa tatgagcatc ggaggccccc
      721 aaacgacgct gtttacctta ctgtttacat tttgaataag ctctacaaaa ttttgcatag
      781 tacctccgaa tgtgttttta ttgaataaca agaaaacggg gcaaaagcag ctaataacag
      841 tattgcttta cgccccgaat tattaagaat aaagctttcc gactatgatg ttcacaagcg
      901 aaaccggcaa tgcctttgaa agtgccaaaa acagatgata tttagtgcct tttgtagaca
      961 aaagcttcgg acgagctttg gttgccgcag catacaccgc atcggctata tcgtccggtg
     1021 acataccgtt ttgctcgtcg cgctccatac cggcaaccgc tcttttaagt gtttcgccgt
     1081 aaaattcgtc accggcggta tttttctctc ttgccgctgt aaaacccgtt ttaacatcgc
     1141 ccggcattac ggcgcagacc tttatattaa acggtcgcac ctcgtttgca agagccagag
     1201 tcagcgaatt tacagccgcc tttgacgccg aataaaacgc ctgaaacggt atggcaagcg
     1261 gagctgcgag agagctgaga ttaacaattc ttccgccgcc ttgaagcctc atatatttaa
     1321 ttacgttttt tgagcaaata aagcagccga agaaattgac gtcaaactgt tttttagctt
     1381 ctgcaagctc tgtg
//
LOCUS       DBMDVO010000430         1394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44531_length_1394_cov_1.263728, whole genome shotgun sequence.
ACCESSION   DBMDVO010000430 DBMDVO010000000
VERSION     DBMDVO010000430.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44531_length_1394_cov_1.263728"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            92..886
                     /locus_tag="ACZPE2_06235"
     CDS             92..886
                     /locus_tag="ACZPE2_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1319483.1"
                     /translation="MPNCKTIAICNQKGGTGKTTTTVNLGIGLAKQGKKVLLVDADPQ
                     GCGSPVETSQAFACEAPTEPTGETLTTCLGWKDNDSLPATITTKLSEIMRKENGNPYF
                     GILHHEENVDLLPANLELSAMEMMLVTTMSRETVMRTYLNKVKDDYEYILIDCMPSLG
                     MVTLNALAAADSVIIPVQAQYLPAKGMTQLMQTIQKVKQHINPSLKIYGILLNIVDNR
                     TNLAKSTADALRKNYGSVIKIYHSAIPMAVKAAEVASKGVSIVRLM"
     gene            874..1080
                     /locus_tag="ACZPE2_06240"
                     /pseudo
     CDS             874..1080
                     /locus_tag="ACZPE2_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
     gene            1171..>1394
                     /locus_tag="ACZPE2_06245"
     CDS             1171..>1394
                     /locus_tag="ACZPE2_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319484.1"
                     /translation="MSDNWVVQNLVVALGVWNQKLTEIWSLITASPQDFKGGDIWNVI
                     VAIYGAVQAIGFALLVLFFVTGMVKTCGSFS"
BASE COUNT          395 a          312 c          338 g          349 t
ORIGIN      
        1 tttacgcagg gggtgttgaa aacaaatact gcgttgatac atagccgtct aaacacagtc
       61 ggctataaat ctatggaaag gaggaaagcc aatgcctaac tgtaaaacca ttgcaatctg
      121 caatcaaaag ggcggcacag gaaaaaccac cacaacggtc aatttaggaa tagggcttgc
      181 gaagcaaggt aaaaaggtac tcctagtaga cgccgatcca caggggtgcg ggtctcctgt
      241 ggagacctca caagcttttg cttgtgaagc accgaccgag ccgacaggcg agaccctcac
      301 gacctgtctc gggtggaaag ataatgacag cttacctgct accattacca ctaagttatc
      361 cgagataatg cgtaaagaaa acggaaaccc gtatttcggt attctgcatc acgaggaaaa
      421 tgttgatttg cttccggcaa accttgaact gtccgctatg gaaatgatgc ttgtaacgac
      481 tatgagccgt gaaacagtta tgagaaccta tcttaacaag gtgaaagatg attacgaata
      541 tatcctgatc gactgtatgc cttccctcgg tatggtaact cttaacgctt tagcggcggc
      601 agacagcgta atcatccccg ttcaggctca gtatcttccc gcaaaaggaa tgacacagct
      661 tatgcagact attcagaagg tcaagcagca tatcaatcca agcctgaaga tttacggtat
      721 cctacttaat attgtagaca accggacaaa ccttgcgaaa agcacggcag atgcgctgcg
      781 gaaaaactac ggaagcgtga ttaagatata tcactcggcc attcctatgg cggtaaaagc
      841 cgcggaggta gcgtctaagg gagtcagcat agtccgctta atgtaatcag caatctgtcg
      901 gactttattt tcggacttat ccgtgcgata ggtcttatcc tgctcggctt cggcatagtt
      961 cagattggtc tttcgcttaa atcgcacgat ccgtctcaga gagctaacgg tttcctgacc
     1021 cttgcaggcg gagtaataat cacctttgca aaggaaatcc ttgaccttat cacaggatga
     1081 aataagcagc ataaacgggg cgtatccgtt tcgggtgcgc cccgaaaata tattgaggca
     1141 atacctcaat aggaaaggat ggttatataa atgagtgata actgggttgt gcaaaatctt
     1201 gtcgttgctc ttggtgtatg gaatcagaag cttaccgaaa tatggtcgct tatcaccgct
     1261 tctccgcagg actttaaagg cggcgatata tggaacgtga tagttgccat atacggtgcg
     1321 gttcaggcta tcggctttgc cctgctcgtc ttgtttttcg ttacgggtat ggtgaaaacc
     1381 tgcggctcgt tttc
//
LOCUS       DBMDVO010000431         1385 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_44904_length_1385_cov_1.542056, whole genome shotgun sequence.
ACCESSION   DBMDVO010000431 DBMDVO010000000
VERSION     DBMDVO010000431.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44904_length_1385_cov_1.542056"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            30..485
                     /gene="dtd"
                     /locus_tag="ACZPE2_06250"
     CDS             30..485
                     /gene="dtd"
                     /locus_tag="ACZPE2_06250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1319485.1"
                     /translation="MKAVLQRVKNAEVTVDGESVGKIGEGYMLLLGVMNGDTKEDAEL
                     LAKKTAMLRVFTDENDKMNKSVLDIGGEVLVVSQFTLCADVKKGNRPSFDNSAKPDEA
                     KALYEYFCSELLKNGVKKVETGVFGAHMQVSLVNNGPVTIIYDTSVWKK"
     gene            497..1129
                     /locus_tag="ACZPE2_06255"
     CDS             497..1129
                     /locus_tag="ACZPE2_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1319486.1"
                     /translation="MSLDIKVMPLGPISANCYILTDKKSRESAVIDPGDFNEALKNAV
                     KDLNIKYILLTHGHFDHILGVCGLKEFTGAKVLIHKLDGDCLSDENKSLCSWEYPGKQ
                     KKMSADILLSDNDKISIGDAVLRVMHTPGHTKGGVCYIDEKDKLIFSGDTLFCLSAGR
                     TDFPGGSTKELFESLKRLRDLSGDYTVCPGHNKATTLDFERKNNRYMRAL"
     gene            1129..>1385
                     /locus_tag="ACZPE2_06260"
     CDS             1129..>1385
                     /locus_tag="ACZPE2_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319487.1"
                     /translation="MKLLIIGHKYQYEMLKLTQIFYPNTKIDLLFSSLDTGDDETVIT
                     TELTKDNVTVSFAEQKKQKVLTKPRPEKEDEERCMASMLFSL"
BASE COUNT          421 a          288 c          339 g          337 t
ORIGIN      
        1 ttttacagcc cccgaaaagg aatatactta tgaaggctgt tttacagcgt gtcaaaaacg
       61 ctgaagttac cgtggacggt gagtccgtcg gaaaaatagg cgaggggtat atgctgcttt
      121 tgggcgtaat gaacggcgac acaaaagagg acgccgaact gcttgccaaa aagaccgcaa
      181 tgctgcgcgt gttcaccgac gaaaacgata aaatgaataa atctgtgctc gatatcggcg
      241 gtgaggtgct tgttgtgtcg caatttacac tctgcgccga cgtaaaaaag ggcaacagac
      301 cgtcttttga caattccgca aagcccgatg aagcaaaggc tctctatgag tatttttgct
      361 cggaacttct gaaaaacgga gttaaaaaag ttgaaaccgg tgttttcggc gcacatatgc
      421 aggtgtcgct tgtaaacaac ggcccggtca caataattta cgatacttcc gtatggaaaa
      481 aataacggag aaaacaatgt cacttgatat aaaggttatg ccgctcggtc cgataagtgc
      541 caactgctat attctgaccg acaaaaaaag ccgcgagagt gcggtaatag acccgggcga
      601 ttttaacgaa gcacttaaaa acgccgtaaa agatttaaac atcaaatata tacttttgac
      661 acacgggcat tttgaccata ttctcggagt ttgcggctta aaggagttta ccggggctaa
      721 ggtgctcata cataagcttg acggcgattg cctgtcggat gaaaataaga gcctgtgctc
      781 atgggagtat ccgggaaagc agaaaaagat gtccgccgac attttgctgt ccgataacga
      841 taaaatatca atcggcgacg ccgttcttcg cgtaatgcac acccccgggc atacaaaggg
      901 cggcgtgtgc tatattgatg aaaaagacaa acttattttt tcgggtgata cgctgttttg
      961 tctcagtgca ggcagaacgg attttcccgg aggcagcaca aaggagcttt tcgagtctct
     1021 taaaaggctg cgcgatttgt cgggcgatta tacggtctgt ccggggcata acaaggcaac
     1081 aaccttggac tttgaacgca aaaataacag atatatgagg gcgctttgat gaagcttctt
     1141 ataattgggc ataaatatca gtatgagatg ttaaagctca ctcagatatt ttatccgaat
     1201 acaaaaatcg acctgctgtt ttcgtcttta gatacgggcg atgacgaaac ggtaatcaca
     1261 accgagctca caaaagataa tgttaccgtg tcgtttgccg agcaaaaaaa gcaaaaggtt
     1321 ctcaccaaac cgcgtcccga aaaagaggac gaagagcggt gtatggcatc tatgcttttt
     1381 tcgct
//
LOCUS       DBMDVO010000432         1381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45064_length_1381_cov_1.488281, whole genome shotgun sequence.
ACCESSION   DBMDVO010000432 DBMDVO010000000
VERSION     DBMDVO010000432.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45064_length_1381_cov_1.488281"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            169..846
                     /locus_tag="ACZPE2_06265"
     CDS             169..846
                     /locus_tag="ACZPE2_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA domain-containing protein"
                     /protein_id="HIR1319488.1"
                     /translation="MADKKKISLGGIDNAAEEKGSFMKALWQFIKFLVVSGLVTIIQL
                     VLANVLPLIFDSVTATLPAFLQGIFAPNTIFDAATAKGVEQIGKYVVGGVIENGTVIG
                     GVVTWGYLLPFFLSNLVANIYGFYQNKKTTFKSDAPWYNFAIYIFLMIALILFSTWFQ
                     GWLVGVIGRSSWGWLQALARTIASLAAGFVQMVVLFPMEKFVLLKEKKKDGEDDADAD
                     KKEEAKA"
     gene            complement(980..>1381)
                     /locus_tag="ACZPE2_06270"
     CDS             complement(980..>1381)
                     /locus_tag="ACZPE2_06270"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1319489.1"
                     /translation="VKTIETKEAHFKPIYDEKLSIKEKLNVLAKEIYRAGDVVFTSNA
                     EKAISEIEKLGKDKLPICVAKTQYSLSDDPQKLGSPKGFTLTVRDVRLSAGAGFIVAL
                     TGDIMTMPGLPKQPAAYKIDVDDDGNVSGLF"
BASE COUNT          357 a          307 c          315 g          402 t
ORIGIN      
        1 ttctttgaaa ggctcttgtg gttcacagga gccttttaat tgtaattatg aataaattgt
       61 gaagttttgt attgcatatt tatacattat ttgttatact aaaagggcta tgtaaaaacc
      121 gtgggagcgt tttatcgctt ccgaaatctc attaagagga gtaaaactat ggcagataag
      181 aaaaaaataa gcctcggcgg tatagacaac gccgcagagg aaaagggctc ttttatgaag
      241 gctctttggc agttcattaa gttccttgta gtaagcggac ttgtcaccat cattcagttg
      301 gtgcttgcaa acgttcttcc gcttatcttc gacagcgtga cagccactct tcccgctttc
      361 cttcagggaa tcttcgcacc gaacacaatc tttgacgcag ctaccgcaaa gggcgttgag
      421 cagataggta aatatgttgt cggcggtgtt atagaaaacg gaaccgtcat aggcggcgta
      481 gttacttggg gctatcttct tccgttcttc ctttcaaacc ttgtagccaa tatctacggc
      541 ttctatcaga acaaaaagac gacatttaaa tctgacgctc cctggtataa cttcgcaatt
      601 tacatattcc ttatgattgc acttatactc ttctcaacct ggttccaggg ctggctcgtc
      661 ggcgttatcg gcagatcctc atggggctgg ttacaggctc tcgcaagaac tattgcgagt
      721 cttgcggcag gctttgttca gatggtagtt ctcttcccga tggagaaatt cgttcttctt
      781 aaagaaaaga agaaagacgg cgaagatgac gcggacgctg acaaaaaaga ggaagctaag
      841 gcataattca accgataaaa aaggttcgcg taagcggacc ttttttgttg cacataaaat
      901 aaaaagcggc tgtaagaacc gaagctctta cagccgtctt tttgttataa atacgaattt
      961 cgattggccg ggtgggttat cagaaaagcc ccgacacatt cccgtcgtcg tcaacgtcta
     1021 ttttatacgc cgcaggctgc ttcgggagcc cgggcattgt cattatgtct cctgtgagag
     1081 ccactataaa tccggcacct gccgacaggc ggacatcgcg taccgtgagt gtgaagccct
     1141 tcggactgcc gagcttttgc gggtcgtccg acagagaata ctgcgttttc gcaacacaaa
     1201 tcgggagctt gtctttgccg agcttttcaa tttcggaaat tgctttttcc gcattcgagg
     1261 tgaatacaac atctccggcg cggtaaatct cctttgcaag aacattcagc ttttccttta
     1321 tcgaaagctt ttcgtcatat atgggcttaa agtgagcttc ttttgtctca atcgttttaa
     1381 c
//
LOCUS       DBMDVO010000433         1381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45067_length_1381_cov_1.399219, whole genome shotgun sequence.
ACCESSION   DBMDVO010000433 DBMDVO010000000
VERSION     DBMDVO010000433.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45067_length_1381_cov_1.399219"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(247..>1381)
                     /locus_tag="ACZPE2_06275"
     CDS             complement(247..>1381)
                     /locus_tag="ACZPE2_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1319490.1"
                     /translation="TESDMETTHFDFRSMHDTILKLDELGHDVPTLDKHLEDMTGINV
                     TDIDICDPEIIKLCTSPEPLGVTPEDISWKTGTLSIPEMGTSFVCQMLLEAQPKTFAD
                     LLQISGLSHGTDVWNGNAQDLIKDGICTISSVIGTRDSIMIYLMHAGLDPSMAFNIME
                     KTRKGIVAKKGFPEGAEEAMREHNVPQWYMDSCRKIKYMFPKAHAAAYVIAALRLGWY
                     KIYKPLEYYTAFLTVRGDGVDAAVAIKGKAAVMARMAEIDDKTRSKTATAKDKEQYTA
                     LQVVNEMMARGIELLPVDIYKSEATRYVIEDGKIRMPFGALSGIGESAAIPLAEARDD
                     GEGKFISVDDFARRAGAGKSTIEMLESVGAFGDIPKTAQLSLF"
BASE COUNT          311 a          358 c          316 g          396 t
ORIGIN      
        1 ttcttttgtg taacccattt taaatctcct ttcaaatata aaaaaagggc agcgagatct
       61 tttaaaagac ctcgttgcct ttcatttatg cattcattat atcaccgaca aggcttttgt
      121 caatgcaaaa tccgtcaaaa aacgtctttt tgtagttttg aacaaaagaa gatgaaaagt
      181 catcgtgttt ataaggcaaa tttgctttaa aaccgtattt cacctttaaa ctgcctcgtc
      241 ggagggctaa aacagtgaca gttgcgccgt tttcgggatg tcgccgaacg caccgacgct
      301 ttcgagcatt tctattgtgg atttgccggc accggcgcgg cgtgcaaaat cgtcaaccga
      361 tataaacttg ccctcaccgt cgtctcttgc ctcggcaagc ggtatggcgg cgctttcgcc
      421 tataccggac agtgctccga acggcattct tattttgccg tcctctatga catatcttgt
      481 agcctcggat ttatatatat cgaccgggag cagctctatg ccccttgcca tcatctcgtt
      541 taccacctgc aaggcggtat actgctcttt gtccttggct gtggcggttt tgcttcttgt
      601 tttatcgtcg atttccgcca tacgcgccat aacggcggct ttacccttta ttgccaccgc
      661 cgcgtcaact ccgtctccgc gcacggtcag aaatgccgta tagtattcga gcggcttgta
      721 tatcttatac cagccgagcc tcagcgcggc aattacatat gccgcggcat gggctttagg
      781 gaacatatac tttatcttgc ggcacgaatc catataccac tgcgggacgt tatgctcacg
      841 catggcttct tcggcaccct cggggaagcc ctttttcgcg actataccct ttctcgtttt
      901 ctccattatg ttaaacgcca tactcgggtc aagccctgcg tgcataaggt aaatcataat
      961 gctgtcacgc gttccgataa ccgaagaaat ggtgcatata ccatccttta taagatcctg
     1021 tgcattaccg ttccaaacat cggttccgtg tgaaagaccg gaaatctgca aaaggtcggc
     1081 aaaggtcttg ggctgtgcct ccaaaagcat ctggcaaaca aacgatgtgc ccatttcggg
     1141 tatagagagc gtgcccgttt tccagcttat atcctcgggc gtcacgccga gcggttcggg
     1201 cgaggtgcag agctttatta tttcggggtc gcaaatatct atgtcggtta cgtttatgcc
     1261 cgtcatatcc tcaaggtgct tgtcgagcgt gggaacatcg tggccgagct cgtcaagctt
     1321 caatatggtg tcgtgcatcg aacggaagtc gaagtgcgtc gtctccatat cgctttcggt
     1381 a
//
LOCUS       DBMDVO010000434         1379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45155_length_1379_cov_1.176056, whole genome shotgun sequence.
ACCESSION   DBMDVO010000434 DBMDVO010000000
VERSION     DBMDVO010000434.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45155_length_1379_cov_1.176056"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>354)
                     /locus_tag="ACZPE2_06280"
     CDS             complement(<1..>354)
                     /locus_tag="ACZPE2_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1319491.1"
                     /translation="FLQKGDTVMTLEKDFSMYSFYTSVVECRHVPYTKNDDYSVDMDD
                     VIATAKKENARIIIFSNPCNPTSRVIGRSDVEKLVSSVDSLVVLDEAYMDFSNQSLLS
                     EFNRYDNLIILKTCSK"
     assembly_gap    355..364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(503..>1379)
                     /gene="hisD"
                     /locus_tag="ACZPE2_06285"
     CDS             complement(503..>1379)
                     /gene="hisD"
                     /locus_tag="ACZPE2_06285"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1319492.1"
                     /translation="PSSVLMNVIPAKIAGVKEIVMVTPPQKDGTSNPDILAAAKIAGV
                     DRVFLMGGAQAVAALAYGTQSVPKVDKIVGPGNIFVATAKKLLYGTVDIDMIAGPSEI
                     LIVADKSANPKFLAADLMSQAEHDKMASAILLTTSEETANETAKELSRQMQTLERRDI
                     IEQSLNDFGAIIVCKDISEAVDFANELAPEHLELAVENPMEYIGRVDNAGSVFLGHYS
                     PEPLGDYFAGPNHVLPTSGTARFFSPLSVDSFIKKSSFIYYTEPALSEAKDDIIKLAE
                     TEGLTAHANSIKVRF"
BASE COUNT          338 a          324 c          330 g          377 t
ORIGIN      
        1 ctttgaacac gtttttaaaa taatcaggtt gtcgtatcgg ttaaactcgg agagtaagct
       61 ctgatttgaa aaatccatat aggcttcgtc gagaaccacg agcgagtcaa cggacgaaac
      121 gagcttttcg acatccgagc gaccgataac gcgcgatgtg gggttgcaag ggttggagaa
      181 aattattata cgcgcgttct ccttttttgc agtcgctatc acatcgtcca tatctacgga
      241 ataatcgtcg tttttggtgt acggtacgtg cctgcactcc acgacagacg tataaaacga
      301 atacatcgaa aaatcctttt caagggtcat tacggtgtcg cccttttgca gaaannnnnn
      361 nnnnagagtt atatatgatt catttgcgtc aagccttatg cggtacgtgc cctcaatcgg
      421 gtcatacggc acaaggtcct ttaccttgcg gttgagtgag aacatttaaa ataccttctt
      481 tacaaaacaa aaatgctccg atttaaaatc taactttgat tgaatttgcg tgagcggtaa
      541 gcccctcggt ctcggcaagc tttatgatgt cgtccttcgc ctctgaaaga gccggttcgg
      601 tataatagat aaagcttgat ttttttatga agctgtcaac cgacagcgga gaaaagaacc
      661 ttgctgtgcc gcttgtaggc aaaacatggt tgggcccggc aaaataatcg ccgagcggct
      721 cgggagaata atggccgaga aaaacgctgc cggcgttatc cactctgccg atgtactcca
      781 tcggattttc gactgcaagc tctaagtgct ccggagccag ttcgttagca aaatcaacag
      841 cctccgatat atccttgcac actattatag cgccgaaatc attgagagac tgctctatta
      901 tgtctctgcg ctcaagagtt tgcatttgac gcgagagctc ctttgccgtt tcgtttgccg
      961 tctcttcact tgtggttaaa agtatggcac ttgccatttt atcgtgctcc gcctggctca
     1021 taaggtcggc ggcgaggaat ttgggatttg cgcttttgtc ggcaactatg aggatttcgc
     1081 tcggccctgc aatcatatca atatccacgg tgccgtagag aagctttttg gcggtagcca
     1141 cgaaaatgtt gccggggccg actattttat cgaccttggg aaccgactgc gtaccgtagg
     1201 caagagccgc aacagcctga gcgccgccca ttaagaatac tctgtcaaca ccggctatct
     1261 ttgcggcggc gagtatatcg gggtttgacg taccgtcctt ttgaggcggt gttaccatta
     1321 caatttcctt aacgccggct atttttgccg gaatgacatt catcagcacg cttgacgga
//
LOCUS       DBMDVO010000435         1379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45163_length_1379_cov_0.922535, whole genome shotgun sequence.
ACCESSION   DBMDVO010000435 DBMDVO010000000
VERSION     DBMDVO010000435.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45163_length_1379_cov_0.922535"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="ACZPE2_06290"
     CDS             <1..308
                     /locus_tag="ACZPE2_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR1319493.1"
                     /translation="ESGLDRLIKAGYSLLGLISYLTAGPKEVRAWTIAKGTKAPQAAG
                     KIHSDFERGFIRAEIVSFDDLMECGSMAAAREKGLVRSEGKDYVMQDGDVTLFRFNV"
     gene            497..1195
                     /locus_tag="ACZPE2_06295"
     CDS             497..1195
                     /locus_tag="ACZPE2_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR1319494.1"
                     /translation="MGFDIYYIILVIPAMIFSFWASVRVNSAFKKYSNVYSRSNMTGR
                     QAAEAVLRQNGVAGVTVVPISGNLTDHYDPTANQIRLSEKVYSSTSCAAIGVAAHEAG
                     HAVQHAEGYLPIKIRSAIIPATNIGSKLAMPLFIIGLMLSYYGEKFLYVAYAGIICFS
                     LCALFQLVTLPAEINASRRAVTAIEGSLLTPEETDGAKKVLTAAALTYVAALAVSLVQ
                     LLRLIAIAGSRRRD"
BASE COUNT          333 a          316 c          389 g          341 t
ORIGIN      
        1 cggagagcgg acttgaccgc cttataaaag ccggatattc tcttctcggg cttataagct
       61 acctgacggc gggaccgaag gaggtccgcg cgtggaccat tgccaaaggt accaaagcgc
      121 ctcaggcggc cggaaagata cactccgatt tcgaacgcgg atttatccgc gctgagatcg
      181 tttcgttcga cgaccttatg gaatgcggtt caatggctgc tgcgcgagag aagggacttg
      241 tccgctccga aggcaaggat tacgtcatgc aggacggaga tgtgactctc ttccgcttca
      301 acgtgtgatc ggacccgaca gtcggtgtcg agccgccgcg tcgtttcccg aggaaatgtg
      361 agccgagagc accggggaat acgggaattt cagacggggc aatatagtgg cgtaaagata
      421 cggcggaatc ggtcggaacc gattgcggag tctttacggg attttcatga ttttttgaat
      481 tgacggaggg cttgaaatgg gatttgatat ttattatatt attcttgtta tacccgcgat
      541 gatattttca ttctgggcga gcgtgcgtgt gaattcggcg tttaagaaat attcgaatgt
      601 ctactcgcgg agcaatatga cgggacgtca ggctgctgag gcggtgctcc gtcagaacgg
      661 ggttgcgggc gttacggttg tcccgatatc cggaaatctt accgaccact atgatcccac
      721 agccaatcag atacgccttt cggagaaagt gtattcgagc acttcgtgcg cggcgatcgg
      781 tgtcgcggct catgaggcgg gacacgccgt tcagcatgcg gaaggatatc tgccgataaa
      841 aataagaagc gcgataatcc ccgccacgaa tatcggatca aagcttgcaa tgccgctttt
      901 cataataggt ctgatgcttt cgtattacgg agagaaattc ctttatgtgg cttatgcggg
      961 cataatatgc ttttcactct gtgcactgtt tcagctcgtc acgcttcccg cggagataaa
     1021 tgcaagccgc cgcgcggtca cagcgataga aggatcgctt cttacacccg aagagaccga
     1081 cggggcgaaa aaagttttga ccgcggcggc gcttacctat gtggcggcgc ttgcggtatc
     1141 gcttgtgcag cttctcagac ttattgccat tgcgggaagc cgccgcagag actgatgggg
     1201 gcggagttgc gcagatattc cgaaaaaacg ggaccgaatg acaaaaaaac tttaccgttt
     1261 gcgcgatact atttttaaga tgaaaggatc actgtcctgc catggaaaag acgtttgata
     1321 aaataaatta cgcaaagctt gtacgcagga tttttcttgt actctttgat attatattt
//
LOCUS       DBMDVO010000436         1378 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45187_length_1378_cov_1.349256, whole genome shotgun sequence.
ACCESSION   DBMDVO010000436 DBMDVO010000000
VERSION     DBMDVO010000436.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45187_length_1378_cov_1.349256"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="ACZPE2_06300"
     CDS             <1..812
                     /locus_tag="ACZPE2_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1319495.1"
                     /translation="ELKDEKGYTFIHPFNDEDVIAGQGTIGLEILDQIPDVDVVIVPI
                     GGGGLISGVAFAIKELNPNVKVYGVQATGAPSMLKSIDDGKIEKLDSVSTIADGIAVK
                     EPGDITFAMCQKYVDGIVTVSDDEIAAAILALIEQQKLITEGAGAVSVAAAMFNKVDV
                     KGKKVCCLLSGGNIDVTILSRVIKRGLLKSGRTYSLTIELLDKPGQLKGVADIIAKEG
                     GNVISIHHERASEGSDINGCYLRIVLETKNFDHIAQIKKALTDAGFIIKSY"
     gene            829..1200
                     /locus_tag="ACZPE2_06305"
     CDS             829..1200
                     /locus_tag="ACZPE2_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1319496.1"
                     /translation="MKTLHTSSAPAAIGPYSQGKIVDGFLFASGQIAINPATGNIEED
                     TIEGQTKRVCENIAAILKEAGADFNSVIKTTCFLKNISDFGAFNEIYGEYFSSKPARS
                     CVGVGDLPKNALVEVEIIAKV"
BASE COUNT          392 a          292 c          326 g          368 t
ORIGIN      
        1 ttgagcttaa agatgaaaag ggctatacgt ttattcatcc gtttaacgat gaggacgtta
       61 tagcgggtca gggtaccata ggacttgaaa tactcgatca gatacccgac gttgacgttg
      121 ttattgttcc cataggcggc ggcggactta tttccggcgt tgcttttgcg ataaaagagc
      181 tcaatccaaa tgttaaggta tacggcgttc aggctaccgg tgctccgagt atgctaaaat
      241 cgattgatga cggtaaaatc gaaaagcttg attccgtttc aaccatagcc gacggtatag
      301 ccgttaaaga gccgggtgac atcaccttcg ctatgtgtca aaaatatgtt gacggtattg
      361 taacagtttc cgatgacgaa atagccgcgg caatacttgc acttatcgag cagcaaaagc
      421 tcattaccga gggcgcaggt gccgtttcgg ttgcagcggc tatgtttaac aaagtagatg
      481 tcaagggcaa aaaggtttgc tgcctccttt cgggcggtaa tatcgacgtt accattttgt
      541 caagagttat taagcgcgga cttttgaaat cgggcagaac atattctctc accattgagc
      601 ttcttgacaa gcccggccag ttaaagggcg ttgccgacat cattgcaaaa gagggcggca
      661 atgttatttc aattcatcat gagcgtgcgt ccgagggctc cgatattaac ggctgttatc
      721 ttcgcattgt tctcgaaacc aaaaactttg accatatagc tcagataaag aaggcactga
      781 ccgacgccgg atttatcatt aagtcatatt aaggaggaaa aacaaatgat aaaaacactt
      841 cacacttcat cggcacctgc cgcaatagga ccgtattcac agggcaaaat cgttgacgga
      901 tttctctttg cttcggggca gatagcaata aaccctgcaa ccggcaacat agaagaagat
      961 acaattgagg gtcagaccaa aagagtttgc gaaaatatcg ctgcgatttt gaaagaggcg
     1021 ggagctgatt tcaacagcgt aattaagaca acctgcttcc ttaaaaacat atccgatttc
     1081 ggtgcgttta acgaaattta cggtgagtat ttttcatcaa aaccggcacg ttcatgcgtc
     1141 ggtgtcggtg accttcctaa aaacgctctt gtggaagttg agattatagc aaaggtataa
     1201 tcaggctttg taaaacctca tttttgttat ttccatttta aaagacaggc cgtaattcgg
     1261 ctgagtgttc ataaagcact tgcctgaaaa taacatttgc acagcagtgc gctaggcccc
     1321 ctcggagatg cgacggcatt atccgagggg gttcgttttt ctcgccttaa attcacgg
//
LOCUS       DBMDVO010000437         1376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45285_length_1376_cov_1.061961, whole genome shotgun sequence.
ACCESSION   DBMDVO010000437 DBMDVO010000000
VERSION     DBMDVO010000437.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45285_length_1376_cov_1.061961"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            110..>1376
                     /locus_tag="ACZPE2_06310"
     CDS             110..>1376
                     /locus_tag="ACZPE2_06310"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1319497.1"
                     /translation="MADGFMGAGMKDTVAYFDLFFRRVPDNGGFAIAAGLSKVIDYLE
                     HLHFREEDIKYLKSKGISDELASYLKDFKFTCDVWAIPEGTPIFPNEPIIKVRGPIIE
                     AQLIETMLLLSINQQSLIATKANRLVRAAHGTAVAEFGTRRAQGVDEAVYGARAAYIG
                     GCVATSGVIPEKEFGIPSVNTMIHSWVQLFDSEYDAFCEYARRHPDDCTLVVDTYDTI
                     RSGIPNAIKAFDDVLAPLGKRPKSVRIDSGDITYLSKRVRKMLDVAGYPDCDIMASNS
                     LDEHLISDMVSQGAKVDCFIVGERLITSASSPLFGGVYKLSAIEKDGKPVPKINLSEN
                     VRKITIPSSKQVYRLIDKDSGKAIADVLTLDDEIIDETKPYILFDPDFTWKRKEIENF
                     VTRKLLVPIFKGGKKVYEEPSLKEIREYCL"
BASE COUNT          408 a          310 c          327 g          331 t
ORIGIN      
        1 ataagctgtt atgatacaat atttataact tatatgagag gtgattattt tggaagatta
       61 tataaaggct tccgatcttg ctctgctgac cgagggctat gagcttacca tggcagacgg
      121 gtttatgggc gcgggtatga aagataccgt agcatatttt gatttattct tcaggcgcgt
      181 tcccgataac ggcggttttg ccatcgcggc aggcctatca aaggttatcg attatcttga
      241 acatctgcat tttcgcgagg aagatataaa atacctcaaa tcaaagggca tctccgacga
      301 gcttgcaagc tatctcaaag atttcaaatt cacctgtgac gtatgggcaa tacccgaggg
      361 cactccgatt tttccgaacg agcctatcat aaaggtaaga ggcccgataa tagaggctca
      421 gcttattgaa acaatgctct tgctctcaat aaatcagcaa agtcttatag ccacaaaggc
      481 aaacaggctt gtaagagcgg cgcacggaac agcggtcgcg gaattcggca caaggcgcgc
      541 tcagggcgtt gacgaagcgg tttacggtgc aagagccgct tacatcggcg gctgtgtcgc
      601 aacatcaggc gtaatacccg aaaaggaatt cggcataccg tcggtcaata caatgataca
      661 ctcatgggta cagctttttg acagcgaata tgacgcattc tgcgaatatg cgagacgtca
      721 ccccgacgac tgcactcttg tggttgacac atatgacacc atccgctccg gtattccaaa
      781 cgcaataaag gcctttgacg atgttttggc tccgctcggc aaacggccga agagcgtaag
      841 aatagattct ggcgacatca catatctttc aaagagagtc agaaaaatgc tcgacgttgc
      901 aggctatccc gactgcgaca ttatggcgtc aaattcgctt gacgagcacc tcataagcga
      961 tatggtatcg cagggtgcaa aggtggactg ctttatagtc ggcgaaagac ttataacctc
     1021 ggcttcttct ccccttttcg gcggcgttta caagctctcc gcgatagaaa aggacggcaa
     1081 accggtgccg aaaataaatc tttcggaaaa cgtcagaaaa attaccattc catcatcaaa
     1141 acaggtttac aggcttatag acaaggattc aggcaaagcg atagcggacg tacttactct
     1201 tgatgacgaa ataatcgacg aaacaaagcc gtatattctt tttgatccgg atttcacctg
     1261 gaagagaaaa gaaatagaaa actttgtgac gagaaaactt ctcgtaccga tatttaaggg
     1321 cggcaaaaag gtctacgagg agccatcgct caaagaaata cgcgaatact gcctga
//
LOCUS       DBMDVO010000438         1374 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45381_length_1374_cov_0.782404, whole genome shotgun sequence.
ACCESSION   DBMDVO010000438 DBMDVO010000000
VERSION     DBMDVO010000438.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45381_length_1374_cov_0.782404"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            72..719
                     /locus_tag="ACZPE2_06315"
     CDS             72..719
                     /locus_tag="ACZPE2_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319498.1"
                     /translation="MDKSGRLTEESIKKCPMNLIRMCGESISSETLCEKLFRNEDMYL
                     GGGGVTFSGGEPLYQSDFLLSCLKILKGRIHTVVQTSGFCDEPIFEKVLAAADYFLFD
                     LKLIDERDHIRYTGVSNKPILKNFEKLAAAEKDFIVRVPLIPGVTDTEKNIEELAKIM
                     NKNGVFYAELMPYNKMAGGKYRSLGREYMPGFDCEAEVNTRKSVFKKYGIEIKIL"
     gene            734..>1374
                     /locus_tag="ACZPE2_06320"
     CDS             734..>1374
                     /locus_tag="ACZPE2_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014900.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HIR1319499.1"
                     /translation="MAKRVDKLLKLLKSKEYKNQREHIEQWDFGNMYDASYQAECFEK
                     VLEREKPLIFENDRMGFNRSTDCTLISAGGNVTPDYGYVISNGFDKIAEEIRENRKKH
                     PENAEFADAMLMCINAAYKTAEKYKTLAKENGNNELYNALCRIPHNGADSFYEACVFL
                     KMSIYFIRADFTNHITLGRFDQYMYPFYLKDKERGVSDEEIFETIEEFFISIN"
BASE COUNT          456 a          230 c          345 g          343 t
ORIGIN      
        1 aatccggaag gtcagaatgc ctgtcgtgag cttattaaaa tccgaacggc tgtatacact
       61 gcggaagctg cgtggataaa agcggcagat tgaccgaaga aagtattaaa aagtgtccga
      121 tgaatctgat acggatgtgc ggcgagagta tcagttcgga gacgctgtgt gagaagcttt
      181 tcagaaatga ggatatgtat ttaggcggtg gcggggtgac cttttccggc ggcgaaccgc
      241 tttatcaaag cgattttttg ttgtcatgcc tcaaaatatt gaaaggcaga atacacacgg
      301 ttgttcagac aagcggcttt tgcgacgagc caatatttga aaaagttctt gcggcagcgg
      361 actattttct ttttgattta aagcttatcg acgaaaggga ccatatccgg tataccgggg
      421 tttcgaataa gccgatatta aaaaattttg aaaagcttgc ggcagcggaa aaggatttta
      481 ttgtgagagt gccgttgata ccgggtgtta ccgataccga aaaaaatata gaagaattgg
      541 caaagatcat gaataaaaac ggcgtttttt atgccgagct tatgccgtac aacaaaatgg
      601 cgggcgggaa atacaggtcg ctcggaagag agtatatgcc cggttttgac tgtgaagccg
      661 aagtgaatac aagaaaaagc gtatttaaaa agtatggcat tgaaataaaa attctgtaag
      721 gagtgtgagt tatatggcaa agagagttga taagctgctg aagctgttga aatcgaagga
      781 gtataaaaat cagagggaac atatagaaca gtgggatttt ggaaatatgt acgacgcaag
      841 ctatcaggca gagtgctttg aaaaagtatt ggagcgcgaa aagccgctta tcttcgaaaa
      901 tgaccgaatg gggtttaatc gaagcaccga ctgtacactt atatcggcgg gcgggaatgt
      961 aacgccggat tatggttatg taatatcgaa cgggtttgac aaaattgccg aagaaattag
     1021 ggaaaaccgc aaaaagcatc ctgaaaatgc agaatttgcc gacgcaatgc taatgtgtat
     1081 caacgcagca tacaaaacgg cggaaaagta taaaacgctt gccaaagaaa acggtaacaa
     1141 tgagctttac aatgctcttt gccgcattcc gcataacgga gcggacagtt tttacgaagc
     1201 gtgcgtattc ttgaaaatgt cgatatactt tatcagagct gacttcacaa accacataac
     1261 gctcggaaga ttcgaccaat atatgtatcc tttctatctt aaggataaag aaagaggcgt
     1321 ttctgacgag gaaatattcg aaacaataga agaatttttc atatcaataa actt
//
LOCUS       DBMDVO010000439         1372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45452_length_1372_cov_1.257278, whole genome shotgun sequence.
ACCESSION   DBMDVO010000439 DBMDVO010000000
VERSION     DBMDVO010000439.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45452_length_1372_cov_1.257278"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="ACZPE2_06325"
     CDS             <1..597
                     /locus_tag="ACZPE2_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR1319500.1"
                     /translation="ACKACGVGFKKEMPSVFHKINDSLWQRLETGEITRDQIHEKRWN
                     MVFSVMGITFDGREFEKEFVKGIYDSHEPVNGAVDTVKYLSKKYILCAASNGPFEQQK
                     HRLTLAGMIDCFSHLFVSERIGIQKPQREFFERCLNELHETDKSKFILIGDSLTADIS
                     GGKSFGITTCWYDHNSTGYDNVGADIRIDSLSEIKSIL"
     gene            607..>1372
                     /locus_tag="ACZPE2_06330"
     CDS             607..>1372
                     /locus_tag="ACZPE2_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013871.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1319501.1"
                     /translation="MIKLPEQVNKAICLLKNSGFEAYAVGGAVRDCLLGKVPFDWDIT
                     TSARPEETEKVFCGERIIETGIKHGTVTVIIDGMPLEITTYRIDGDYTDHRHPQTVSF
                     SGNLRDDLSRRDFTINTLCCNCDDKIIDAFGGKDDLNNKIIRCVGDPDTRFSEDALRI
                     MRAVRFSAELGFKIEKSTSDSILKNRQLLSDVSKERLQVEFTKLITGKYATDVIRKYH
                     AVIEVFIPELAPLVECEQHTKYHKYDVFEHTLHAVDA"
BASE COUNT          431 a          301 c          333 g          307 t
ORIGIN      
        1 gcttgcaaag cttgcggagt aggtttcaaa aaagaaatgc cgtcggtttt tcataaaatc
       61 aacgactctc tctggcaaag actcgaaacg ggagaaatca caagggatca aatacacgaa
      121 aaacggtgga atatggtttt ttcggttatg ggtatcactt ttgacggacg cgaatttgaa
      181 aaagaattcg taaaaggaat atatgacagc cacgagcctg taaacggagc tgtggatacg
      241 gttaaatatc tgtcaaaaaa atacatactc tgtgccgcaa gcaacgggcc gtttgagcag
      301 caaaaacacc gactgacact tgccggaatg atagactgct tttctcacct ttttgtttcg
      361 gagagaatag gcatacaaaa gccccaacgg gaattttttg agcggtgcct caatgagctg
      421 cacgaaaccg acaaatcaaa atttatactt ataggcgatt cgcttaccgc cgatatatcc
      481 ggagggaaga gcttcggaat caccacctgt tggtatgacc acaacagcac ggggtatgat
      541 aatgtgggcg cggatataag aatcgacagt ctctcagaaa tcaaaagcat actttgagga
      601 attattatga taaagctccc cgaacaagtg aataaggcga tatgcctgct gaaaaacagc
      661 ggttttgaag cctatgccgt cggcggcgcg gttcgtgatt gtctgctcgg caaagtcccg
      721 tttgactggg acatcacaac aagcgcacgc cccgaggaaa ccgaaaaggt gttttgcggc
      781 gaacggataa tagagacagg tataaagcac gggacggtaa ctgttataat tgacggtatg
      841 ccgctcgaaa taaccactta tcgcatagac ggcgactata cagaccaccg tcacccgcaa
      901 acggtatcat tttcgggtaa cctcagagac gatctctcaa ggcgggattt cacaataaac
      961 acgctttgct gtaattgcga cgataaaata attgatgctt tcggcggcaa agacgatttg
     1021 aacaacaaaa taatacggtg cgtcggcgac cccgacaccc gtttttcgga ggacgcgctc
     1081 agaataatgc gtgccgtccg tttttcggcg gagctcgggt tcaaaataga aaaaagcaca
     1141 tcggacagca tactgaaaaa caggcagctg ctgtcggatg tgtcaaaaga aagattacag
     1201 gttgaattca caaagcttat cacgggaaaa tacgcaaccg acgttatacg gaaatatcac
     1261 gcggttatag aggtatttat cccggagctt gccccacttg tagaatgcga gcagcacaca
     1321 aaatatcaca aatacgatgt gttcgagcat acgcttcacg ccgtagacgc ga
//
LOCUS       DBMDVO010000440         1372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45468_length_1372_cov_0.889851, whole genome shotgun sequence.
ACCESSION   DBMDVO010000440 DBMDVO010000000
VERSION     DBMDVO010000440.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45468_length_1372_cov_0.889851"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(183..1208)
                     /locus_tag="ACZPE2_06335"
     CDS             complement(183..1208)
                     /locus_tag="ACZPE2_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319502.1"
                     /translation="MEKYISKKEKASYGFAAVGSYMVAGVTQSYLMYFFTDILIIPSA
                     FVIVLMIVARVWDALNDPMMGVIVDRTSTKYGKMRPYVLIGSFLMLVTTIVLFMPLTG
                     APTMFKMIFAAVSYIIFGMAYTLTDVPAMGLMSVATPNSDERASLLSFYVTVGSVGSL
                     LPVGLLTIFRSFIPEKYVYFAMSIFVGIITSAAYLTLFKNSKERFSTHTEKIAVRDMI
                     KAAGRNKPMMALLMSMTASPRYVIMPAAMYIATYVINIPGFNSFVTKFNNAVGFMGLS
                     AALTLFHFIEPSAAGVPAVQSASTVTGLFSLVTVIPGIGFLLSLIPICFYDFSGVKKE
                     KIYLSLP"
BASE COUNT          418 a          293 c          277 g          384 t
ORIGIN      
        1 atattacagc cctgtttttt gagtacggaa agcgataaga caagattgtt tcggaacgtc
       61 gtctcgtttt ttctgcgaac agggctgagc tgggaatcgg aaattatgcc gaccctcagc
      121 ctttcgttaa tattaaaggt ttttcgttca aatttaaact ccatttttga ttgcctttct
      181 ttttacggca agctcagata aattttttct ttcttaacgc cgctgaaatc ataaaaacaa
      241 atcggtataa gcgagagcaa aaagccgatc ccgggaatta cggtaacaag agagaaaaga
      301 cccgtaacgg tggaggcact ctgtacagcc ggcacacccg ccgcggacgg ctcgataaaa
      361 tgaaacaaag taagtgcagc agaaagtccc ataaagccta ctgcattgtt gaatttagtg
      421 acgaaggagt tgaaccccgg aatatttatt acataagtcg ctatatacat tgccgccggc
      481 ataataacgt aacgcggcga cgccgtcatc gacataagaa gtgccatcat gggcttattt
      541 ctgcccgcag cctttatcat gtctcgaacg gcaatttttt cggtatgagt ggaaaaacgc
      601 tcttttgagt ttttgaacag cgttaagtaa gccgccgatg ttattatacc cacaaatatc
      661 gacattgcaa agtaaacata tttttccgga ataaaagacc ggaatatcgt aagcaatccc
      721 accgggagca agctacctac cgagcctacc gtaacgtaga acgacagaag cgaggctctc
      781 tcgtcgctgt tcggcgtagc aacactcata agccccatgg caggcacatc ggtaagcgta
      841 tatgccatac cgaaaattat gtaagaaacg gcggcaaata tcattttaaa cattgtaggc
      901 gctccggtca agggcataaa cagtactatg gtggtgacaa gcatcaaaaa cgaacctatc
      961 aaaacataag gcctcatttt accgtatttc gtgcttgttc tgtctactat tacacccatc
     1021 atagggtcgt ttaaggcatc ccataccctt gcgactatca taagcactat aacgaaagcc
     1081 gacggaatta taagtatgtc ggtaaaaaaa tacataaggt atgattgggt tacgccggct
     1141 accatatagc tgccgactgc cgcaaatccg taggaggctt tttccttttt agagatatat
     1201 ttttccatat tttctctttt tttgcaattc atctttattt tttattttat aaaaaaatta
     1261 tattcgtcga aaacacgttg taacaatcaa gacttcgtta agtctttgta aacttgtgta
     1321 aaaactttgc tttgtaacac attgaataag ccggcataag atgttattga ag
//
LOCUS       DBMDVO010000441         1371 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45492_length_1371_cov_1.403937, whole genome shotgun sequence.
ACCESSION   DBMDVO010000441 DBMDVO010000000
VERSION     DBMDVO010000441.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45492_length_1371_cov_1.403937"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="ACZPE2_06340"
     CDS             <1..625
                     /locus_tag="ACZPE2_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1319503.1"
                     /translation="FEADFARAVCEKLGVTPTFQVISWEAKESELNSKNVDCLWNGLT
                     ITDARKNTMSISTPYMANKQVLITKSENADKYSSAGSLKGATVVAEKESAGEEVATSD
                     AFFEGANYTAVDSMAKAIMEVSSGTADAAVIDYVTGIGSIGEGTNYTNIVMVSGANFA
                     DEEYGIAFRKDSDITPEVNKAIDELAKDGTLRTIAEKYKLGGQLLVG"
     gene            701..1348
                     /locus_tag="ACZPE2_06345"
     CDS             701..1348
                     /locus_tag="ACZPE2_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1319504.1"
                     /translation="MDSFVNISIELLKGFCENLKLFSLTLLFSVPAGLLVMFCSISKF
                     KPLKWLSKTFVWIIRGTPLMLQLFVVLYVPGLLFNTPIKVRFTAALIAFVINYACYFS
                     EIYRGGIESISKGQYEAGQVLGMTKPQIFFKVILLQVVKRIVPPMSNEVITLVKDTSL
                     ARVIAVAEIIMAADRFSARGIIWPLFYTGAFFLIFNGLLTVIFNKIEKKLDYFRG"
BASE COUNT          387 a          265 c          335 g          384 t
ORIGIN      
        1 ctttgaggct gattttgcaa gagctgtttg cgaaaaactc ggtgttacgc ctactttcca
       61 ggtaataagc tgggaggcaa aagagagcga gctcaattca aaaaatgttg actgcctttg
      121 gaacggcctt acaataaccg acgcgagaaa aaacaccatg tctatctcaa cgccctatat
      181 ggcaaataaa caggttctca taacaaagag cgaaaatgcc gataaatatt cttcggcagg
      241 ttcgttaaag ggtgcaacgg tagttgccga aaaagaatcc gcaggtgaag aggttgcaac
      301 aagcgacgca ttctttgagg gcgcaaacta cacggcggtt gactcaatgg caaaggcgat
      361 aatggaggtt tcctccggca cggcagatgc cgctgttata gactatgtta ccggcatcgg
      421 ttcaataggc gagggcacca actatacaaa tatagttatg gtaagcggcg cgaattttgc
      481 ggatgaggaa tacggtatcg ctttcagaaa ggacagcgat ataactcccg aggttaacaa
      541 agcaatcgac gagcttgcaa aggacggtac tctcagaacc atcgcagaaa aatataaact
      601 cggcggtcag ctccttgtag gctgaaaaaa ctaaaaaata actttttgag gcggcggcgc
      661 gtctgcgctt gccgtctaaa agtttgttta aggtgaagtt atggactctt ttgtgaatat
      721 atcaatagag cttttaaaag ggttctgcga gaatttgaag ctgttttcgc tgacgcttct
      781 gttttctgtt cccgcaggac ttcttgttat gttttgctct atatcaaaat ttaagccgct
      841 gaagtggctt tctaagacct ttgtatggat aattcgcggc acgccgctta tgctccagct
      901 ttttgttgtg ctgtatgttc cggggctttt gtttaacaca cctattaaag tgcgttttac
      961 ggcggctctg atagcgtttg taataaatta cgcctgctat ttttccgaga tttaccgcgg
     1021 cggtattgag agcatttcaa aaggtcagta cgaggcaggg caggttctcg gtatgacaaa
     1081 gccgcaaata ttttttaagg taatactttt gcaggtggta aagagaatag ttccgccgat
     1141 gagcaacgaa gtaataaccc ttgtaaaaga tacttccttg gcaagggtaa tagccgttgc
     1201 ggaaataata atggctgccg acaggttttc ggcacgcgga ataatttggc cgctgttcta
     1261 tacaggcgcg tttttcttga tatttaacgg cttgctgact gttatcttta ataagattga
     1321 aaagaaactc gattatttca gaggttaatt tatgactgta cttgaagtaa a
//
LOCUS       DBMDVO010000442         1368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45629_length_1368_cov_1.552486, whole genome shotgun sequence.
ACCESSION   DBMDVO010000442 DBMDVO010000000
VERSION     DBMDVO010000442.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45629_length_1368_cov_1.552486"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="ACZPE2_06350"
     CDS             <1..307
                     /locus_tag="ACZPE2_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319505.1"
                     /translation="PFDVALVVADYLSDSVIPLSDIVSSIKRINRPKDPKKIESGDYR
                     IVISYYPDIYSSDYIYGRYYFAKGYKCSLKDEGYFHYWGESSPLGRFLEDTPLISIN"
     gene            333..1001
                     /locus_tag="ACZPE2_06355"
     CDS             333..1001
                     /locus_tag="ACZPE2_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319506.1"
                     /translation="MNNLFENWNDTASWTTDGSLQYDERNHVLYFDKTKVTEKLSDVN
                     EDYDVAAVRTFEIPDGSHGFYFSVTVGNSSPLFDNNGGRIDVEFYDANGVKTVGDYST
                     FYIYNAENFIEAYLGDDKPAPIPEDAKSMVIKAYGVSDTKTIEFYMKDFKLSFSDSED
                     CMETFANVYPTFFSRKVDNSPIRISSGSQIMTIVSFAAIIAGVAIVTVLLARADRRKR
                     KTGK"
     gene            1011..>1368
                     /locus_tag="ACZPE2_06360"
     CDS             1011..>1368
                     /locus_tag="ACZPE2_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319507.1"
                     /translation="MKKYAVITIVLLIMLIGGCSAGNKSLSDGSSENSVIYDSSAPED
                     DVKYTYVCAQYIYYNTADELMKACDIVMSGKVTGISFTVRDGRTNDEVTSDTSASDKE
                     ICTVYTVEKEAVYKNTV"
BASE COUNT          434 a          222 c          340 g          372 t
ORIGIN      
        1 tcctttcgat gttgccttag tagttgctga ttacttgagt gatagtgtta ttcctctatc
       61 tgacatcgtt tcatcaataa aaagaataaa tcgaccgaaa gatcccaaaa aaattgagag
      121 tggtgactat agaattgtga tatcgtatta tccagatatt tatagcagcg attacatata
      181 tggaagatat tattttgcaa aagggtataa gtgttctctg aaagatgaag gctatttcca
      241 ttattggggt gaaagtagcc ctttgggaag atttttagaa gatactccat tgatcagtat
      301 aaactagttt aatgcgatag aggtgtcgaa aattgaataa tttatttgaa aactggaatg
      361 atacagcaag ttggacaacg gacggctcac tgcaatacga tgaaagaaac catgtgctgt
      421 attttgacaa gacaaaggtt acggaaaagt tgtccgatgt aaacgaagac tatgatgttg
      481 cggcagtgcg gacgtttgaa atacctgacg gcagtcatgg attttatttt tcggtgacgg
      541 taggaaacag ctcgccgctg tttgataaca acggcggacg tatagacgtt gaattttatg
      601 acgcaaacgg tgtaaagacg gtaggagatt attcaacttt ttacatatat aatgcggaga
      661 atttcatcga ggcttatctg ggcgatgaca aacctgcacc tatcccggaa gacgcaaaaa
      721 gtatggttat aaaagcgtac ggggtttccg atacaaaaac gatagaattc tatatgaagg
      781 attttaaact gtcgttttcg gacagtgagg actgtatgga aacattcgct aatgtttatc
      841 cgacgttttt cagcagaaag gttgataatt ctccgataag aataagctcg ggctcgcaga
      901 tcatgactat agtcagcttt gcggcaataa tcgcgggtgt tgcgatcgtg acggtgctgc
      961 ttgcaagagc ggacagaaga aaacggaaaa cgggaaagta aggggtgagt atgaaaaaat
     1021 atgcggtaat tacgattgtt ctgcttataa tgctgatcgg cggatgcagt gccggcaata
     1081 aatcgctttc tgacggctcg tctgaaaaca gcgttatata tgacagcagt gcgcccgaag
     1141 acgatgttaa gtacacttat gtctgcgctc agtacatata ttacaacaca gccgatgaat
     1201 tgatgaaagc gtgcgatata gtaatgtcgg gcaaggttac gggaatatca ttcacggtaa
     1261 gggacggcag aacgaatgac gaggttacca gtgatacgtc tgcaagcgac aaggaaatct
     1321 gtacggttta tacggtcgag aaggaagctg tatataaaaa tacagtcg
//
LOCUS       DBMDVO010000443         1367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45681_length_1367_cov_0.949447, whole genome shotgun sequence.
ACCESSION   DBMDVO010000443 DBMDVO010000000
VERSION     DBMDVO010000443.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45681_length_1367_cov_0.949447"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(364..876)
                     /locus_tag="ACZPE2_06365"
     CDS             complement(364..876)
                     /locus_tag="ACZPE2_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1319508.1"
                     /translation="MTVHFVNCDFMIALLLENKTAAEAAQKIQGLKKKLKDNNFSFGD
                     IIPVLLTDNGGEFSIVSAFEDDEFGNRETNLFFCEPSSPNEKPEIEKNHTLFRDIVPA
                     GHSFDAFTQQDVNLIFSHINAVKRKQFGGKSAYDLFTFFNSVELAQILGISYISPNDV
                     IQSPLLLKKL"
     gene            complement(916..>1367)
                     /locus_tag="ACZPE2_06370"
     CDS             complement(916..>1367)
                     /locus_tag="ACZPE2_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR1319509.1"
                     /translation="YCPKLLKAPFVCNGCEMKSRAKCPYIRRIYVAKTAQSSYEETLV
                     ESRSGIPLNKESFYETEQIISNAVKQGQHVYHAIKANNLPISTATVYRHINKQYYSIG
                     RIDLPRAVKFKPRKTTAPEHIPACAKKGRTYQDFIGFMEDNDALPLA"
BASE COUNT          442 a          260 c          264 g          401 t
ORIGIN      
        1 gtacgaaact ttcggactta ttgaaaccgt ggaaaaagcg gcgcagcttt atatgcttac
       61 ggcgcatctg ccgcatatca acaccatcca tgacgatgaa ttaaaggcgg ttgccgacgc
      121 tttcgggctt aaatacagaa cggatttcct cgatctttaa aaaaatcgta aattctcaaa
      181 gtaaatgcaa cagtacgaaa aggttgcatt catcgtgaaa aataaaggag ttaagtgtga
      241 gaaaaagcgg gggaaacagg ctaaaaagcc ttaaaagggc aaaaaagtgc aacccaaata
      301 agcccgagaa acaaatttct cagactaact gcaacaatag atgcatttac tatgagaata
      361 aaatcaaagc tttttgagca ataacggaga ttgaattaca tcgtttggag aaatataaga
      421 aatgcctaaa atttgtgcta attctactga attgaaaaat gtaaacaaat cataagcact
      481 tttaccgccg aattgttttc gctttacagc atttatgtgc gaaaaaatca aattaacatc
      541 ttgttgcgta aaagcatcga aagaatgtcc tgccggaaca atatcacgaa acaatgtgtg
      601 gtttttttca atttccggct tttcgttagg tgaagatggc tcacaaaaga acaaattggt
      661 ttccctgttg ccaaattcgt catcttcaaa agcggaaaca atactgaatt caccgccgtt
      721 atctgtaagc aatacaggaa taatatcacc gaaagaaaag ttgttatctt ttagcttctt
      781 ttttaatcct tgaatttttt gtgcggcttc ggcagcggtc ttgttttcaa ggagcaaagc
      841 tatcataaaa tcacaattaa caaaatgaac agtcataata gttttgccgc ctactcgacc
      901 tattacagtg tctaattaag ccaatggcaa ggcatcgtta tcctccataa aaccgataaa
      961 atcttggtag gtacgccctt ttttcgcgca agcaggtata tgttcgggtg cggttgtctt
     1021 tctcggcttg aattttacgg cacgaggtaa atcaattctt ccgatggaat aatattgctt
     1081 gttaatgtgc ctgtatacag tagcggttga aatcggaaga ttatttgctt taatagcatg
     1141 gtaaacatgt tgtccctgtt tgactgcatt tgaaataatc tgctcggttt cataaaaact
     1201 ttctttattt aagggtatac cgcttcgaga ttccaccaaa gtttcctcgt aagagctttg
     1261 agcggttttt gcaacataaa ttcggcgaat atacggacac ttcgcacggc ttttcatttc
     1321 acacccatta caaacaaacg gtgctttcag aagcttcgga cagtatt
//
LOCUS       DBMDVO010000444         1361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45939_length_1361_cov_1.423016, whole genome shotgun sequence.
ACCESSION   DBMDVO010000444 DBMDVO010000000
VERSION     DBMDVO010000444.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45939_length_1361_cov_1.423016"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(41..811)
                     /locus_tag="ACZPE2_06375"
     CDS             complement(41..811)
                     /locus_tag="ACZPE2_06375"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR1319510.1"
                     /translation="MATTVDLNCDMGESFGAYKIGLDEEVAKYISSANVACGFHASDP
                     VVMDKTVKILKENNVNLGAHPGYPDLMGFGRRNMNVSPADAKAYVMYQIGALSAFAKA
                     NGLKIQHVKPHGALYNTAGKDYALSKAICEGIYEVDPSLILLGLSGSQMLKAAADTGL
                     KCAKEVFADRAYEEDGSLVARTKPGAVITDEDEAIKRVIGMVKHGKVTAITGKEIPIE
                     ANSICVHGDGAKALEFVMKIRAALTIENIKIAPLCEIV"
     gene            complement(827..>1361)
                     /locus_tag="ACZPE2_06380"
     CDS             complement(827..>1361)
                     /locus_tag="ACZPE2_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glutamate cyclase family protein"
                     /protein_id="HIR1319511.1"
                     /translation="PKYRIYEKGVMTGEYDSVEQFWRDDLVAFLIGCSFSFESELLDS
                     GITVRHIEEHKNVPMYLTNIDCEPAGVFSGKMVVSMRPLPRDQIVKAVNITSSMPRVH
                     GTPIQIGHPEQIGISDINKPDFGDAVTVKSGETPVFWACGVTPQSIMMNSKPDFCITH
                     APGHMLITDIKNTDLKF"
BASE COUNT          304 a          337 c          318 g          402 t
ORIGIN      
        1 ttggcgtaaa ctctctgtgg gagcatattt caaaccttct ttatactatt tcgcacagcg
       61 gtgcgatttt aatgttttct atggtcaatg cggctcttat tttcattacg aattccaaag
      121 cctttgctcc gtctccgtga acgcatatag agtttgcctc tatcggaatt tccttgccgg
      181 ttatcgcggt taccttgccg tgctttacca ttccgataac gcgctttatg gcttcgtcct
      241 cgtcggttat gactgcgccc ggctttgtcc ttgcaacaag agagccatcc tcctcatacg
      301 ccctgtcggc aaaaacctcc ttggcgcatt taagaccggt gtcggcggcg gctttaagca
      361 tttgactgcc tgaaagaccg agaagtataa ggctcgggtc aacttcgtat attccctcgc
      421 atatcgcctt tgacagcgca tagtccttgc cggcggtgtt gtaaagcgca ccgtgaggct
      481 ttacgtgctg aattttaaga ccgttagcct ttgcaaaagc cgacagagcg cctatctgat
      541 acattacgta agcctttgca tctgcggggc ttacgttcat attgcgcctg ccgaagccca
      601 taagatcggg gtagccgggg tgtgcgccga gattaacgtt gttttcttta agtattttaa
      661 cagtcttatc cataaccacg gggtcggaag cgtgaaatcc gcaggctacg tttgccgatg
      721 agatgtattt tgcaacctct tcgtccagac ctattttata tgcgccgaag ctctcgccca
      781 tatcgcagtt taaatctact gttgttgcca tttttatctc ctcccgttaa aatttgagat
      841 cggtattttt aatgtcggta ataagcatgt gacccggagc atgcgttatg caaaagtcgg
      901 gctttgaatt catcattata gactgcggag ttacgccgca agcccaaaat acaggtgttt
      961 cacccgactt tacggtaact gcgtcgccga aatcgggttt gtttatatca cttatgccta
     1021 tctgctcggg atgacctatc tgaataggcg tgccgtgtac gcgcggcata gaggaggtga
     1081 tgttgaccgc ttttacaatc tggtcgcggg gcaggggacg catacttaca accatcttgc
     1141 cggaaaatac gccggcgggt tcgcagtcga tatttgtaag atacatcgga acatttttgt
     1201 gctcctcaat gtgacgtacc gtaataccgc tgtccaaaag ttcgctttca aacgaaaagc
     1261 tgcagcctat gaggaacgct acaaggtcgt cgcgccaaaa ttgctctacg ctgtcatatt
     1321 cgccggtcat aacacccttt tcatatattc tgtatttggg a
//
LOCUS       DBMDVO010000445         1361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_45949_length_1361_cov_1.210317, whole genome shotgun sequence.
ACCESSION   DBMDVO010000445 DBMDVO010000000
VERSION     DBMDVO010000445.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45949_length_1361_cov_1.210317"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="ACZPE2_06385"
     CDS             complement(<1..374)
                     /locus_tag="ACZPE2_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319512.1"
                     /translation="MSTIIDGLSLGSIYALIALGYTMVYGIAKMLNFAHGDIIMVGAY
                     AILTTIMAFGNVPLAILVSVIVCTVLGILTEKAAYKPLRGASPLAVLITAIGVSYLLQ
                     SIAQIIYGPQSKPVTIAEPKRI"
     gene            complement(497..>1361)
                     /locus_tag="ACZPE2_06390"
     CDS             complement(497..>1361)
                     /locus_tag="ACZPE2_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1319513.1"
                     /translation="AATGYDALYEAGMNASIGSVTSGSCDSFGAKAAEDNLFFITPSA
                     SAANVIEGRKNGFRVCFGDPDQGTLAAEELTKKFTKIGAIYDTSDPYSSGIFEAFDEE
                     MKKLGVEYTVRTFDAENKKDFSTQVEAMADCEVIFIPIYYTEAGLIARAAVAKGIGAE
                     LFGCDGLDGIADQVDASVTNTIKYITPFNVNSKDDNVVKFVTAYQAKYNALPDQFAAD
                     AYDAMYTLAQAMEKAGLSDPTISPSDLTDAIIPVITGSDFKVVGVTGTMTWDESGACS
                     KEPQIVTLGEE"
BASE COUNT          336 a          337 c          322 g          366 t
ORIGIN      
        1 ttgattctct taggctcggc aatcgtcacg ggctttgact gtgggccgta tattatctgt
       61 gcgatgctct gtaataaata gctgacgccg attgcggtga taagtaccgc aagcggagaa
      121 gctcccctca aaggtttgta tgcagctttt tcggtcagaa ttcccaaaac tgtgcagact
      181 atcacggaga ccaaaattgc aaggggcacg tttccgaatg ccataatcgt tgttaaaatt
      241 gcatacgcac cgaccatgat aatatcgccg tgcgcaaagt tcaacatctt tgcaattccg
      301 tataccattg tatagccaag cgcaataaga gcatatatac tgccgaggct caagccgtca
      361 attatagtgc tcataaaaat ctcctttcag ggcagccctg caagtcacct tgcatagcgc
      421 cggtaaaatt taaaacagat tttggtcggg cggcaaaatt tcatgccgcc caaccaaaac
      481 gccgttcgtt ttaatcttat tcttcgccga gtgtaacgat ctgaggctcc ttcgagcaag
      541 cgcccgattc atcccaagtc attgttccgg taacaccgac aaccttaaag tcagagccgg
      601 tgattacggg aatgatcgca tcggtaaggt cagacggtga aattgtagga tcggataatc
      661 ctgccttctc cattgcctgt gcaagtgtgt acatagcgtc atacgcgtct gccgcaaact
      721 ggtcgggaag tgcattgtac ttagcctggt atgcggtaac aaacttaaca acattatcgt
      781 ccttgctgtt tacgttgaag ggagtgatgt acttgatcgt gtttgtaacg cttgcgtcaa
      841 cctggtctgc gataccgtca agtccgtcgc atccgaagag ctccgcaccg attccctttg
      901 ctacagcagc tcttgcgata aggcctgctt ccgtgtagta gatgggaatg aaaataactt
      961 cgcagtcagc cattgcttca acctgagtcg agaagtcctt cttattctcc gcgtcgaaag
     1021 ttcttaccgt gtattcaacg ccgagtttct tcatctcttc gtcaaatgcc tcgaagatac
     1081 ctgaagaata cggatcgctt gtatcataga tagcgccgat cttggtgaat ttctttgtaa
     1141 gctcttcagc tgccaaggta ccctgatcgg ggtcgccgaa gcaaactctg aatccgttct
     1201 ttcttccttc aattacgttt gccgcggaag cggagggagt aatgaagaag aggttatcct
     1261 ctgctgcctt tgccccgaaa gagtcgcagg agccggaggt tacgctaccg atagaagcgt
     1321 tcatgcctgc ttcgtaaaga gcgtcatatc ctgtagcggc g
//
LOCUS       DBMDVO010000446         1356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46185_length_1356_cov_1.081275, whole genome shotgun sequence.
ACCESSION   DBMDVO010000446 DBMDVO010000000
VERSION     DBMDVO010000446.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46185_length_1356_cov_1.081275"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>695
                     /locus_tag="ACZPE2_06395"
     CDS             <1..>695
                     /locus_tag="ACZPE2_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP3 family relaxase"
                     /protein_id="HIR1319514.1"
                     /translation="EVSDNVRKKLIHDTFANELSYYYGISNEAMSDATELVGKLMIDF
                     ERDISGIYSNRYSGVDIPEELIQKYTDVLNNLPGRGRIAYAYLPKETKDMVIGMIKEM
                     IMLPKMKSIINDYYSGARSAAEMFDSKETDNGKSEIDRKMRKAAGDLYNRLGNIILKS
                     MLRTLKENNEQRKKDNAEINTKITVDSMVYALNAVSRLFRTQGSAGTVNTAHALIGEL
                     SKQAKKDIARQKQ"
     assembly_gap    696..705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            730..1065
                     /locus_tag="ACZPE2_06400"
     CDS             730..1065
                     /locus_tag="ACZPE2_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319515.1"
                     /translation="METQIYFDFKNNKLSLKGVNAGYKLLEHNEFTDVDGTLIGVVLA
                     KSKTQYATWRYSFYPKSEYFTGLNLGHYFSNEISAKTDYFERLSEMGNRLKSEISKTC
                     CETDEDMEI"
     gene            1181..>1356
                     /locus_tag="ACZPE2_06405"
     CDS             1181..>1356
                     /locus_tag="ACZPE2_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024361.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3848 domain-containing protein"
                     /protein_id="HIR1319516.1"
                     /translation="MNELKAQLIKNMQAELDKLKEDLLNISSQEILSRAYEYTMKTEI
                     IYAAHDAKLNHYQI"
BASE COUNT          485 a          238 c          307 g          316 t
ORIGIN      
        1 gaggtatcgg ataatgtaag aaaaaaactg atacacgata cattcgcaaa tgagctgtcg
       61 tattattacg gcataagcaa cgaagcaatg tcggacgcaa cagagcttgt cgggaagctg
      121 atgattgatt tcgagcgtga catttccggt atctattcca atcgttactc cggagttgat
      181 atacccgaag aattgataca gaagtacacc gatgtattga acaacttgcc ggggcgagga
      241 agaatagcct atgcatatct tccgaaagaa acgaaggata tggttatcgg tatgataaaa
      301 gaaatgatta tgttgccaaa gatgaaatct atcattaatg actattattc gggtgcaaga
      361 tcggcggcag aaatgttcga cagtaaagaa accgataatg gtaaatccga aattgacagg
      421 aaaatgcgga aagccgcagg cgatttgtat aacaggctcg gcaacattat actgaaatca
      481 atgctcagaa ctttgaaaga gaacaatgag cagagaaaaa aggataatgc cgagataaac
      541 acaaagataa ctgttgacag tatggtatat gctttgaacg cagtgagcag attgtttaga
      601 acacaaggct ctgccggaac ggtaaacact gctcacgctc tgatcggtga gctgtcaaag
      661 caggctaaga aagacattgc aaggcaaaaa cagcannnnn nnnnngataa aggacacgag
      721 gtgtaatcta tggaaactca gatatatttt gatttcaaaa acaataagct gtcgcttaaa
      781 ggagtaaatg ccggatataa attacttgag cataatgagt ttaccgatgt tgacggcacg
      841 ttaataggcg tggtgttagc aaaaagtaaa actcagtatg ctacctggag atattcattt
      901 taccccaaga gtgaatactt caccgggctt aacttaggtc actatttcag caatgaaatt
      961 tctgctaaaa cagactattt tgagcggtta tccgaaatgg gcaatagact aaagtcagag
     1021 ataagcaaaa cctgttgtga aacagatgaa gatatggaaa tctgaatact tagtaacata
     1081 agtgcgacac gatgtcgcac cggacgttcc ggtcgcacag gacgctcctg tcgcacagga
     1141 cgctcctgtc gcacttaaaa taaaggaggt acaaattaaa atgaacgaat taaaggcaca
     1201 gcttatcaag aatatgcagg cagagcttga caagctgaaa gaagacttgc tgaatatctc
     1261 gtcacaggaa atactcagca gagcatatga atatacgatg aaaacggaaa taatctatgc
     1321 agcacacgat gctaagctta atcattatca gattaa
//
LOCUS       DBMDVO010000447         1355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46234_length_1355_cov_0.998405, whole genome shotgun sequence.
ACCESSION   DBMDVO010000447 DBMDVO010000000
VERSION     DBMDVO010000447.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46234_length_1355_cov_0.998405"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="ACZPE2_06410"
     CDS             complement(<1..980)
                     /locus_tag="ACZPE2_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1319517.1"
                     /translation="MLPWHLTASPLHLLSLFFSPITNLRDDEYGGDEERRMKAPLDVL
                     RAIRKAVGKDYPVIVRIAGSQMMPGGYGIDYMQKFCSTAEKEGLIDAVNVTGGWHEAP
                     VPQISYQLPAGGFAYLADAIKRAVNVPVIACNRINNIDTMEAILKNGMADFVGTARGF
                     LADPEFSRKVLEGVPYNRCQGCNKCIEAVLKGKSVVCAYNPEAGREVEEKTHRRVISA
                     KKVIVAGAGPAGLEAAKKSAERGYKTILCTKDDKLGGLLNTAAIPPEKQDLRCFIENK
                     KYELEQLGVDIRLNTEVTGALIHEIQPYFVVDATGGDVVIPPIKGIDKDNV"
     gene            935..>1355
                     /locus_tag="ACZPE2_06415"
     CDS             935..>1355
                     /locus_tag="ACZPE2_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR1319518.1"
                     /translation="MKVNAMEMQSSAREAYEKGKEELKTTRTSVKNAISSLNGLSKIN
                     QLAMEILSIANKTNLLSLNASIEAARAGEAGKGFAVVAGEIGALADTSKLTATNIQQI
                     CAEANDSIDSVNNCFNDMMGYMEQSVVRQFENFASQST"
BASE COUNT          382 a          293 c          283 g          397 t
ORIGIN      
        1 acattatctt tgtcaatgcc ctttataggt ggaataacaa catctccgcc ggttgcgtca
       61 acaacaaaat acggctgtat ttcatgtatt aatgctcctg taacctctgt atttaaacgt
      121 atgtcaacac caagctgctc aagctcatat ttcttgtttt cgatgaaaca gcgtaaatcc
      181 tgtttttcgg gaggaattgc ggctgtgttc aaaagaccgc caagcttatc gtcctttgtg
      241 cagagaattg ttttatagcc tctttctgcc gatttttttg cagcctcaag tccggcaggg
      301 ccggctccgg caacaataac ctttttagca gatataactc ttctgtgtgt cttttcttca
      361 acttctctgc cggcttcggg attatatgca caaacaacgc tcttgccttt aagaacggcc
      421 tcaatacact tattacagcc ctgacaacgg ttataaggta caccttcaag aaccttgcgt
      481 gaaaattccg gatcggcaag gaaacctctt gccgttccta caaaatcagc cattccgttc
      541 tttaatattg cttccattgt atctatgttg ttaattctgt tacaggcaat tacgggaaca
      601 ttgacagctc tctttattgc atcggcgaga taagcgaatc cgcctgccgg aagctgatag
      661 gaaatctgcg gtacaggagc ttcatgccag ccgcctgtta cgtttaccgc atcaataaga
      721 ccttcttttt ccgctgttga acagaatttc tgcatataat caatgccata accgccgggc
      781 atcatctgtg aaccggcaat acgtactata acgggataat cctttccgac tgcttttctg
      841 attgctctta atacatcaag aggagctttc attcgtcttt cttcgtctcc gccgtattca
      901 tcgtcacgaa gatttgttat aggtgagaag aaaagtgaaa gtaaatgcaa tggagatgca
      961 gtcaagtgcc agggaagcat atgaaaaagg aaaggaagag ctgaaaacaa ccagaacttc
     1021 tgttaaaaat gcaatttcca gtctgaatgg gttatcaaaa attaatcagc tggcaatgga
     1081 aatactgagt attgcaaata aaacgaatct tttatctctt aatgcgtcca ttgaagcagc
     1141 aagagccggc gaggccggaa agggctttgc tgttgttgcg ggtgaaatcg gcgcacttgc
     1201 tgatacatcc aaattaacgg caaccaatat ccagcagatt tgtgcggaag caaatgacag
     1261 tattgacagt gttaataatt gctttaatga tatgatgggt tatatggaac aaagtgttgt
     1321 acggcagttt gaaaattttg caagtcagtc aacgg
//
LOCUS       DBMDVO010000448         1351 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46419_length_1351_cov_1.224000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000448 DBMDVO010000000
VERSION     DBMDVO010000448.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46419_length_1351_cov_1.224000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            322..>1351
                     /locus_tag="ACZPE2_06420"
     CDS             322..>1351
                     /locus_tag="ACZPE2_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR1319519.1"
                     /translation="MFKKFKRLSRKRQIFTVLISVIIVALVVALLYQTFKPEPRPEYQ
                     VQAASVGDIQATFDTKGTVESTSTEKFTAAAGVKVSSVNVAVGDRVEAGDVLATFDVS
                     SIDGTLANYKSAFDKAKAAYDKASGTVSSAKSGIKAIDLQISQTESDISALKREIAAT
                     HNADIKNAENAKKYTEEQLEALREQLKNGGLTEEEIQDIINSLKNSSGDVDIEKALSD
                     SVAAKQMKLAQAEAQLTSLKTQRAVYEAQTDETVSDIYKSVMEQKQKDYESYKAIYDS
                     LKNGWVASADGIITTVNIEAGKEFTPADTGSSGVDLSSIIGSMTSNSEVASTLTDILS
                     STAGSASSV"
BASE COUNT          411 a          287 c          306 g          347 t
ORIGIN      
        1 cttttacatt ccttgaaaaa ctcctcatac ttgatttcgg gagaatcgta ttccgaattg
       61 tagcggcggc aaaattcata tgcgtcaatg ctctcttgcc gtgagcaggc ggttagacaa
      121 acagctgaca ggcttaccga tatgaaaaag caaaaaaact gcctgcatag cattttgcac
      181 gttttgtgtt tattaactgt ttgttcatac ttttttaaca aaaacatatg gcattctgtc
      241 ctttaatagt ataaaattat taggtatact caattacttt aaaatattat attccaatca
      301 aaatgcgaaa gtggtggtat tttgtttaag aaattcaaaa gattatcccg aaaaaggcag
      361 atttttacgg ttttaatttc ggtaattata gttgcgcttg tggtagcact tctctatcag
      421 accttcaagc cggagccgag acccgaatat caggtgcagg cagcctctgt cggagatata
      481 caggcgacat ttgatacaaa ggggacggtt gagtccacaa gcaccgaaaa attcacggcg
      541 gcggcaggcg tcaaggtaag ttccgtcaat gttgcggtgg gcgacagagt agaggcgggg
      601 gatgttcttg ctacctttga tgtatcttca atagacggta cgctcgcaaa ttataaaagt
      661 gcctttgaca aagccaaggc ggcatacgac aaagcaagcg gtaccgtgag ctccgcaaaa
      721 agcggtataa aggcaatcga ccttcagatt tctcagacgg aaagcgatat ttccgcgctt
      781 aaaagagaaa tagccgcaac acataatgcc gatataaaaa atgccgagaa cgcaaaaaaa
      841 tataccgaag aacagcttga ggctcttcgc gagcaactta aaaacggcgg acttaccgag
      901 gaagagatac aggatataat caattccctt aaaaattcct ccggcgatgt agatatagaa
      961 aaggctcttt cagattccgt tgccgcaaag cagatgaaat tagcccaggc ggaagcacag
     1021 ctgacctcgc ttaaaactca gcgcgccgtt tatgaggctc agacagacga aaccgtgtct
     1081 gatatttata aatccgttat ggagcaaaag caaaaggatt atgagagcta taaagctata
     1141 tatgattccc ttaaaaacgg ttgggtcgct tcggcggacg gaataatcac cactgtcaat
     1201 attgaggcgg gcaaggagtt tacgcccgcg gatacaggtt cctccggcgt tgatctctct
     1261 tcgattatcg gctctatgac gagcaattcc gaggtagcct caactcttac cgatattctt
     1321 tcgtcaacag caggctccgc ttcgtctgtc g
//
LOCUS       DBMDVO010000449         1342 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46849_length_1342_cov_1.219984, whole genome shotgun sequence.
ACCESSION   DBMDVO010000449 DBMDVO010000000
VERSION     DBMDVO010000449.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46849_length_1342_cov_1.219984"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="ACZPE2_06425"
     CDS             <1..344
                     /locus_tag="ACZPE2_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1319520.1"
                     /translation="VAGLKTPLAMIMFGTYLSKTNLKTMFLKKDTYLVSFLKLIALPA
                     VILAVLKLMGVKGTLLTALIISASAPSANNTVMFAAKYGKDSAAASQVVAVASLLSII
                     TMPLFIALSQI"
     gene            380..703
                     /locus_tag="ACZPE2_06430"
     CDS             380..703
                     /locus_tag="ACZPE2_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1319521.1"
                     /translation="MKLLIAIVNNEDASLVISELNKAGFMVTKLATSGGFLRAGNVTM
                     LTGVEDEKVGDALAVIEEFSSQRKQITPVNSTYIGDSMLTVPVEVSVGGATVFVVDVE
                     QFYKI"
     gene            700..>1342
                     /locus_tag="ACZPE2_06435"
     CDS             700..>1342
                     /locus_tag="ACZPE2_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319522.1"
                     /translation="MTADISSFGESFKKEVLPYINTLSFPNTMLIEGGSAELRLSAAR
                     FIAQSLLCVSSGAHPCGVCPSCVKCEALSHPDMREYGDISSDAAFKVEACRAVRSDCF
                     VLPNDGDKKVYILKEVQNMNDSGENALLKIFEEPPSYVYFIMTCPSRSAMLDTVLSRA
                     TVFSLGSENDSALFDEDTADLAQKIAKALLSPAETSLLAAVSPLEKDRTGFKNT"
BASE COUNT          326 a          305 c          354 g          357 t
ORIGIN      
        1 acgttgccgg tcttaagacg ccgcttgcca tgataatgtt cggcacatat ctttcaaaaa
       61 caaatctgaa aacgatgttt ttaaaaaagg acacttatct cgtatcgttt ttaaagctca
      121 tagctttgcc ggctgtgatt ttggcggtgc tgaagcttat gggcgtaaag ggaacacttt
      181 tgacggcgct tataatttcc gcgtcggcac cgagtgcaaa caataccgtt atgtttgcgg
      241 caaagtacgg caaggacagt gccgcggcct cacaggtcgt cgccgtggca agccttctgt
      301 cgataataac aatgccgctg tttatagcac tttcgcaaat ataaattcat atgtcgtttt
      361 aaattgaaag gaggacatta tgaaactttt aatagctatt gtcaacaacg aggatgcatc
      421 cttggtcatc tccgagctta acaaggcggg ctttatggta acaaagcttg ccacctccgg
      481 cggatttctc cgtgcgggta acgtcaccat gctcacgggt gtggaggacg aaaaggtcgg
      541 tgacgcgctt gccgtaatag aggaattttc aagtcagcgc aagcagatta ctcccgttaa
      601 ttcaacatat ataggcgatt ccatgctaac cgttcccgtt gaggtttcgg ttggcggagc
      661 cacggtcttt gtggtggatg tcgagcagtt ctataagata tgacggcaga catttcttca
      721 ttcggcgaga gctttaaaaa agaggttttg ccgtatataa acaccttatc gttccctaat
      781 acaatgctta ttgagggcgg ctctgccgag ctgcggcttt cggcggcgcg ttttatagcg
      841 cagagcctgc tttgcgtgag cagcggcgca catccgtgcg gtgtttgccc gtcctgcgtg
      901 aaatgtgagg ctttgtcgca cccggatatg cgtgaatacg gagacataag ctccgacgcg
      961 gcgtttaagg tcgaagcctg ccgcgccgtg cgctccgatt gctttgtcct gccgaatgac
     1021 ggtgacaaaa aggtgtacat tttaaaagaa gttcaaaata tgaacgacag cggtgagaac
     1081 gcacttctca aaatatttga ggagccgccc tcatacgtgt attttattat gacgtgtccg
     1141 agccgctcgg ctatgcttga cacggtgctg tcgcgtgcga cggtattttc gctcgggtct
     1201 gaaaatgact cggctctttt tgacgaggat accgccgatt tggcgcaaaa aatagctaag
     1261 gcgctgttaa gtcccgcgga aacgtctctt ttggctgcgg tgagccctct tgaaaaggac
     1321 aggacgggat ttaaaaatac gg
//
LOCUS       DBMDVO010000450         1341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46907_length_1341_cov_0.809677, whole genome shotgun sequence.
ACCESSION   DBMDVO010000450 DBMDVO010000000
VERSION     DBMDVO010000450.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46907_length_1341_cov_0.809677"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="ACZPE2_06440"
     CDS             complement(<1..808)
                     /locus_tag="ACZPE2_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012286.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR1319523.1"
                     /translation="MKLGVIGGAGLLGSTSAYLAGMKGYLDEIKLVDIKENLAKCHVM
                     DMGQAFAAFSKTKVSYAEYKDLGDCDIILISASIPERKVNDRMEYLRDNMKIVKPICE
                     QIKANCKEAVIINATNPVDIFNYIIWKILGWDRKKILGFNGNDTYRLKWATAEITGKE
                     FGKIDGFCIGEHGANKINLYDQMTYDGKPLVLSDADKKAIEDVTANFFAEFQALDSGR
                     TSGWTSSINIEELIEAVATNSGKKIECSSVLLGEYGYEDVSFGIPVSVGKE"
     gene            complement(821..>1341)
                     /locus_tag="ACZPE2_06445"
     CDS             complement(821..>1341)
                     /locus_tag="ACZPE2_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1319524.1"
                     /translation="GARLAYALASEKNDLDFKTIAANCDVFYIGGTKCGALFGEAVVI
                     TNDTLKQDFRYMIKQKGGMLAKGWLLGVQFSELLRNGLYLKLAERADRLAIKIRNAAL
                     ENGYKLYYDSYTNQQFFVMSNKTYTELSKKYVFSTEAKPDNDHIAVRICTDWATKEEN
                     VDQLIKDMAIYK"
BASE COUNT          319 a          307 c          267 g          448 t
ORIGIN      
        1 cttcttttcc gactgaaaca ggtattccga aggatacatc ctcatagccg tattcaccta
       61 aaagaacaga agaacattct attttctttc cggaatttgt tgcaacggct tcaatgagtt
      121 cttctatgtt tattgaagaa gtccaaccgg aagttcttcc gctgtcgaga gcctgaaatt
      181 ccgcaaagaa gtttgctgtt acgtcctcta ttgccttttt gtctgcgtcc gaaagaacga
      241 gaggctttcc gtcgtaggtc atctgatcat agaggtttat tttgttggct ccatgctctc
      301 cgatacagaa gccgtctatt ttgccgaatt ccttacctgt tatttctgct gttgcccatt
      361 taagacggta ggtgtcgttt ccgttaaagc cgagaatttt ctttctgtcc cagccgagaa
      421 ttttccagat tatatagttg aatatgtcaa cgggattggt tgcgtttatt ataacagcct
      481 ctttgcagtt ggcttttatc tgctcacata tgggctttac tatcttcatg ttgtcacgga
      541 gatattccat tctgtcgttt acctttcgct ccggtatgct tgcggaaatc agaatgatgt
      601 cgcaatcgcc gagatcctta tattcggcat agctgacctt tgtttttgaa aaagccgcaa
      661 atgcctgtcc catatccatt acatgacatt tggcaaggtt ttccttgatg tcaacaagct
      721 tgatttcgtc aagatagccc ttcatgcctg caagatacgc agaggttgag ccgagaagtc
      781 cggctccgcc gataacaccg agtttcataa aattcctcct ttatttatag atagccatat
      841 cctttattaa ttggtctacg ttttcctctt ttgttgccca gtctgtgcat atacgaaccg
      901 caatgtggtc gttgtccggc tttgcctctg ttgagaaaac atattttttg ctcaattcgg
      961 tataggtttt gtttgacata acgaaaaact gctggtttgt atagctgtca tagtaaagct
     1021 tatagccgtt ttccaaagcc gcatttctta tttttattgc aagcctgtcc gctctttccg
     1081 caagctttaa ataaagtccg ttacgaagaa gctccgagaa ctgaacaccc agaagccagc
     1141 ctttggcgag cattccgccc ttctgcttaa tcatgtatcg gaaatcctgt ttaagagtat
     1201 cgtttgtaat gacaacagcc tctccgaaca atgcaccgca ttttgttccg cctatataaa
     1261 atacatcgca gtttgcggct attgttttaa aatcaaggtc gttcttttcg cttgcaaggg
     1321 cataggcaag ccttgcaccg t
//
LOCUS       DBMDVO010000451         1339 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46968_length_1339_cov_2.089661, whole genome shotgun sequence.
ACCESSION   DBMDVO010000451 DBMDVO010000000
VERSION     DBMDVO010000451.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46968_length_1339_cov_2.089661"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1339
                     /locus_tag="ACZPE2_06450"
     CDS             <1..>1339
                     /locus_tag="ACZPE2_06450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319525.1"
                     /translation="LSWQLTLLVILMLIIMLIVIKVIGGKSALYFKKQQKDLGAVNGY
                     IEETIEGQKVIKVFNHEENVKEEFAALNGALKHSSTNANTFASVLMPIMGNLSNINYA
                     VIAMAGGLLCIGGFTTIGTISSFLQYSRSFSQPIANLSQQFNNILAALAGAERIFDVI
                     DREPEEDNGYVKLVNAKYDENGNLTECKEHTGLWAWKHPHSGDNTVTYQKLEGDVVFE
                     NVYFSYDGKKTVLDDISLYAKPGQKIAFVGSTGAGKTTITNLLNRFYEIQKGKIRYDG
                     INIQKICKGDLRSSLSMVLQDTHLFTGTIEDNIRYGKLDATHEEIVKAAKLANADSFI
                     TRLPDGYDTVITGDGANLSQGQRQLIAIARAAVADPPVLVLDEATSSIDTRTEHLIEK
                     GMDKLMNGRTTFVIAHRLSTVRNADAIMVLEHGKIIERGSHDDLIKQHGKYYQL"
BASE COUNT          392 a          309 c          329 g          309 t
ORIGIN      
        1 ctcagctggc agctcactct tcttgtaatt ctgatgctta taattatgct gattgtaatt
       61 aaggttatag gcggcaagag cgcgctgtac ttcaagaagc agcaaaagga cctcggagct
      121 gtaaacggat atatcgaaga gaccatagag ggacaaaagg tcataaaggt attcaatcac
      181 gaggaaaatg tcaaggagga gtttgccgca cttaacggtg cgctcaagca ttcctctacc
      241 aacgccaata cctttgcaag tgttctgatg ccgataatgg gtaacctctc aaatatcaat
      301 tatgccgtga ttgccatggc aggcggactt ctctgcatag gcggctttac cacaatcggt
      361 acaataagct cgtttttgca gtattcaaga tcgttttctc agcccatagc aaatctctct
      421 cagcagttca acaacattct tgcggcctta gccggtgccg agcgtatatt tgacgttatc
      481 gacagagagc cggaagagga caacggctat gtaaagctcg taaacgcaaa atatgacgag
      541 aacggtaatt tgaccgagtg caaggagcat actggccttt gggcttggaa gcatccgcac
      601 agcggcgaca acaccgtaac ctatcaaaag cttgagggcg acgttgtgtt tgaaaacgtg
      661 tacttcagct atgacggcaa aaagacggtt ctcgacgaca tttcgctcta tgcaaagccg
      721 ggtcagaaaa ttgcgtttgt cggctcaacg ggcgcaggca aaaccacaat aaccaatttg
      781 ctcaaccgct tttatgagat acaaaagggg aaaatccgtt atgacggaat aaatatacaa
      841 aagatttgca agggtgacct tcgttcttcg ctttcgatgg tgcttcagga tacacacctg
      901 tttacgggta caatcgaaga caatatacgc tacggcaagc ttgacgcgac tcacgaagag
      961 attgtaaaag ccgctaagct cgccaatgcc gattcgttta taacaagact tcccgacggt
     1021 tacgacacgg ttataacagg cgacggcgca aacctgagtc aggggcaaag acagctcata
     1081 gcgattgcga gagcggctgt ggcagacccg cccgtgctcg tgcttgacga ggctacaagc
     1141 tccatagata cacgcaccga gcatcttata gaaaagggca tggataagct tatgaacggc
     1201 agaaccacct ttgtgatagc tcaccgtttg tccacggtac gcaatgcgga cgccataatg
     1261 gtgcttgagc acggtaaaat aatagaacgc ggcagtcatg atgaccttat taagcagcac
     1321 gggaaatatt atcagctgt
//
LOCUS       DBMDVO010000452         1339 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_46983_length_1339_cov_1.192246, whole genome shotgun sequence.
ACCESSION   DBMDVO010000452 DBMDVO010000000
VERSION     DBMDVO010000452.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46983_length_1339_cov_1.192246"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="ACZPE2_06455"
     CDS             complement(<1..1219)
                     /locus_tag="ACZPE2_06455"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1319526.1"
                     /translation="MGYTVAQKLIKSHLVSGEMKVGSEVGLKIDQTLTQDATGTMAYL
                     EFEAMGIKRVKTELSVAYVDHNTLQTGFEDADDHRFIGSVAKKRGIRFSRPGNGICHQ
                     VHLERFGKPGKTLIGSDSHTPTGGGIGMLAMGAGGLDVAVAMGGGTYYITMPKIVNVH
                     LTGKLSPYVTAKDVILEVLRRMTVKGGVGKIIEYTGEGVKSLSVPERATITNMGAELG
                     ATTSVFPSDEITKAFLEAQGRVDDWTELSADSDAVYDEFIDIDLSSLEPLAAMPHMPD
                     KVKKVSEIGKIKIDQVCIGSCTNSSLMDMLKVAAILKGHTVAENVSLSIAPGSKQVLN
                     MLALNGALSDMINAGARILESACGPCIGMGQSPNSKGISLRTFNRNFKGRSGTNDAQI
                     YLVSPETAAISAIE"
BASE COUNT          343 a          317 c          266 g          413 t
ORIGIN      
        1 cttctatagc tgatatagcg gcagtctcag ggcttacaag gtatatctgc gcgtcgttcg
       61 tgccgctcct tcccttgaag tttctgttga acgttctgag agaaatgcct tttgaattag
      121 gcgactgacc catacctata catggtccgc aagcgctttc gagaattctg gcacccgcat
      181 ttatcatatc cgagagtgct ccgttgagtg caagcatatt aagcacctgc tttgaacccg
      241 gagcgatgct caggctgacg ttttcggcaa cggtatgtcc ctttaatata gccgcgactt
      301 tcagcatatc cattaaagag gaatttgtgc atgagcctat gcatacctga tcaattttaa
      361 ttttgcctat ttcagaaacc ttttttactt tatcgggcat atgcggcata gctgcaagag
      421 gctcaagaga agaaaggtct atatcaataa attcgtcgta taccgcatcg ctgtcggcag
      481 aaagctctgt ccaatcatca actctgcctt gtgcttcaag aaacgccttg gttatttcat
      541 ctgacgggaa aaccgacgtt gttgctccaa gctcagcacc catattagtt atagttgctc
      601 tctccggaac ggataaagat ttaacgcctt cgcctgtata ctcgattatt ttgcctacac
      661 cgcccttaac agtcattctt ctgaggactt caaggattac gtctttggcc gtaacataag
      721 gactgagttt cccggtaaga tgtacattga cgattttcgg catggtaata tagtatgtgc
      781 cgccgcccat tgcaacagct acgtcaagtc cacctgcacc catagcgagc atacctatac
      841 cgccgcctgt gggtgtgtgg ctgtcagagc caataagcgt ttttccgggt ttgccgaaac
      901 gttctaagtg tacttgatgg caaataccgt ttcccggacg agaaaatcta attccgcgtt
      961 tctttgcaac actgcctata aaacgatggt catctgcatc ttcaaatcct gtctgcaagg
     1021 tattatggtc aacataagca accgaaagtt ctgttttaac tcttttaatg cccatggctt
     1081 caaattcaag atatgccata gtgccggttg catcctgagt aagagtctgg tctattttga
     1141 gaccgacctc actgccgact ttcatttctc cgctgacaag atgtgactta ataagctttt
     1201 gagctactgt gtaacccata tagatcgctc ctaacttttt cacattttta acaatcttat
     1261 tttaatccat ataaaatact ttgtcaatga attatcaagt gaattttcat catataacct
     1321 gattttcttt aataatagg
//
LOCUS       DBMDVO010000453         1336 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47128_length_1336_cov_1.203239, whole genome shotgun sequence.
ACCESSION   DBMDVO010000453 DBMDVO010000000
VERSION     DBMDVO010000453.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47128_length_1336_cov_1.203239"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(195..635)
                     /locus_tag="ACZPE2_06460"
     CDS             complement(195..635)
                     /locus_tag="ACZPE2_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319527.1"
                     /translation="MKEKSRTEEFLNKVYENAEMCCESISMITEKVEDPSLLSELQSE
                     HRDYTDLSGEAVNLLAKENKLPKEKGPLAQFGVWSGIQMNTAFDKSPDKIAEMMMQGS
                     MMGVIDMSRTLKEYGDTPEDCRKLGENLIKLEENSIQKMKQYLG"
     gene            779..>1336
                     /locus_tag="ACZPE2_06465"
     CDS             779..>1336
                     /locus_tag="ACZPE2_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1319528.1"
                     /translation="MGKLNKFTAFMGNKFQPVFGKIVYRNMRRKPIVPKELKRLSDLA
                     PLDKDKVLLVAHRGLSGAYPENTAPSFEAAGKHGGYFGLECDTHMTRDGVWVIMHDPD
                     VSTIYSGTGDVKELSFDEIERMHVARGSNADKFPGLKACTLQEYIDICKKYNCHPIIE
                     IKDPRKDKMQDFYNVLLKNDLLKDAV"
BASE COUNT          372 a          314 c          271 g          379 t
ORIGIN      
        1 accttctttt ccataatata tactctgtta ttataagata taaccaaatc tatgtcaaca
       61 aaaaaggacg tttaaaaaga cctaatcgcc gattacgcgg acgtttcgga gcaagcgaaa
      121 ttttcaacgt tttcagccga aatcgagcga ggcgtaaaac tgcccgattt tttacgcccc
      181 acccgatatt acaattaacc gaggtactgt ttcatttttt gtatgctgtt ttcctcaagc
      241 ttaatcaaat tctcaccgag ttttcggcaa tcctcagggg tatcgccgta ttcctttagg
      301 gttcgcgaca tatctataac gcccatcatg ctgccctgca tcatcatttc ggcgatttta
      361 tcggggcttt tgtcaaatgc cgtattcatc tgtatacccg accaaactcc gaactgcgcc
      421 agcggtccct tttccttagg caatttgttt tcctttgcga gcagatttac agcctcgccc
      481 gacaaatcgg tataatccct gtgctcgctc tgaagctccg acaaaaggct cgggtcctca
      541 accttttcgg ttatcattga aatggattcg cagcacatct cggcattttc gtatacctta
      601 tttaaaaatt cttcggttct gcttttttcc ttcaagtgta tcttccttcc ttttttgccg
      661 aaaaaacgga tttttttatt attatcccaa agcaacaggg ctttatactg tttacatttt
      721 aaggttttta aactataata tgtattaaac agcagtaaga aaggttaagg tgattttcat
      781 gggcaagctt aacaaattca cagcgtttat gggaaataaa ttccagccgg tattcggcaa
      841 gatagtatat agaaatatgc ggaggaaacc gatagttccg aaagagctta aaagactctc
      901 ggacttagca ccgctcgata aggacaaggt tctgctcgta gcgcaccgcg gactttcggg
      961 agcatatccc gaaaacaccg ctccgtcgtt tgaggcggca ggcaagcatg gcggatattt
     1021 cggccttgaa tgcgacacgc acatgacaag agacggcgtt tgggtaatta tgcatgaccc
     1081 ggacgtttcg accatataca gcggcacggg cgacgttaaa gagctttcat ttgacgaaat
     1141 tgaaagaatg cacgtcgcaa gaggctccaa tgccgataaa ttccccggac tcaaagcttg
     1201 cacgcttcag gaatacatcg acatctgcaa aaagtataat tgccacccga taatcgaaat
     1261 caaggatccg cgcaaggaca aaatgcagga tttctacaat gtgcttctca aaaacgatct
     1321 gcttaaggac gccgtt
//
LOCUS       DBMDVO010000454         1335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47193_length_1335_cov_1.064019, whole genome shotgun sequence.
ACCESSION   DBMDVO010000454 DBMDVO010000000
VERSION     DBMDVO010000454.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47193_length_1335_cov_1.064019"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1335)
                     /locus_tag="ACZPE2_06470"
     CDS             complement(<1..>1335)
                     /locus_tag="ACZPE2_06470"
                     /inference="COORDINATES: protein motif:HMM:NF036809.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR1319529.1"
                     /translation="LIDTALFSNINMIRIWGGGYYESDKFYNLCDKKGILVWQDFQFA
                     CQAYPFFDNDFLDNVKEEVKYNVKRLCHHPSLAVWNGNNEIEDMHMAWVHMQKYVKWT
                     EKFFYHILEPEIRKYDKNTPYTPGSPVGESHNVGVESDNVGDTHLWGVWHGLKPMNYY
                     RKRMTRFCSEFGFESLPDMKAIEKFAKPSDYSLSSDVFKSHQKCANGNDKMIYYIASR
                     FNLPKEFKDYVYLSQVTQNECIADATEHWRRNKGRCNGSMYWQFNDCWGVCSWSSLDY
                     YGNYKALQYGAKHFNAPLSISIENTSDYIRVFVLNDLNESKTVDAEYEIFDFENGTLE
                     TNKKTLTVGAVKNSVVFDLNVPFICSEYDKKRTGIAVRLYENSALIQQKTVLFCRENE
                     LRLPKAELKTVFEETADGLKVTVSSDKYARLVKVESSKSTLPFSDNFFDLLP"
BASE COUNT          371 a          280 c          257 g          427 t
ORIGIN      
        1 ccggaagcag gtcaaaaaag ttatcgctga acggcaaagt gcttttgctg ctttcaactt
       61 tgacaaggcg agcatatttg tcggatgaca cagtaacctt caatccgtcc gccgtttcct
      121 caaaaacagt tttaagctcg gcttttggca agcgaagctc gttttcgcgg cagaaaagaa
      181 cagttttttg ctgaataagg gcactatttt cataaagtct tactgcaatg cctgttcttt
      241 ttttatcgta ttccgagcaa atgaacggaa catttaaatc aaaaacaaca gaatttttga
      301 cggcaccgac agttaatgtt tttttgtttg tttcaagagt tccgttttca aaatcaaata
      361 tttcatattc cgcatcaact gttttgcttt cgtttaaatc attcagcaca aaaacacgta
      421 tatagtcgct tgtattctca atcgatatgc tgagcggcgc attaaagtgc tttgcaccgt
      481 attgtaaggc cttgtaattg ccatagtaat caagacttga ccaagagcat acaccccagc
      541 agtcattaaa ctgccaatac attgaaccgt tgcatctgcc cttgtttctg cgccaatgtt
      601 cggtggcatc tgcgatacat tcgttttgcg ttacctgtga caaatagaca taatccttga
      661 actcctttgg taaattaaac ctagatgcta tataatatat cattttgtca ttgccgttgg
      721 cacatttttg gtgagactta aacacatcgc tcgaaaggga gtagtcggaa ggctttgcaa
      781 acttttcgat agccttcata tcaggcaggc tttcaaagcc gaattcgctg caaaaacgtg
      841 tcatacgttt gcggtaataa ttcatcggct tcaaaccgtg ccatacgccc cacagatggg
      901 tgtcgcctac attgtcgctt tctactccga cgttatgaga ttcacctaca ggcgaaccgg
      961 gtgtgtatgg agtgtttttg tcatattttc ttatttcggg ttcaagtata tgatagaaga
     1021 atttctcggt ccatttgaca tacttttgca tatgtaccca agccatatgc atatcttcaa
     1081 tttcattgtt gccgttccat acagcgagag agggatgatg acagaggcgt tttacattgt
     1141 atttaacctc ttcttttaca ttgtcaagaa agtcattatc aaagaacgga taggcttgac
     1201 aggcgaattg aaaatcttgc caaacaagta ttcctttttt gtcacagaga ttataaaact
     1261 tgtcgctttc atagtatccg ccgccccaaa tgcgtatcat atttatattt gaaaacaggg
     1321 ctgtgtcaat taaat
//
LOCUS       DBMDVO010000455         1332 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47354_length_1332_cov_0.757920, whole genome shotgun sequence.
ACCESSION   DBMDVO010000455 DBMDVO010000000
VERSION     DBMDVO010000455.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47354_length_1332_cov_0.757920"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="ACZPE2_06475"
     CDS             complement(<1..716)
                     /locus_tag="ACZPE2_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1319530.1"
                     /translation="MLKKKTERLIPQSDQPKKQVSRGKVTVPKNSKQNALSNEKNSLE
                     NRKTAKKPTSGKKNSAGQKKQNPQKTKNQNSKTQQKSVQKNGTSRPVKNERKTTKATG
                     NSKGQSKRRGKKNNVPLKIAFLGGLNEVGKNMTLYECGNDMMLVDCGLEFPDTEMLGV
                     DLVIPDFTYVTENASKIRGIVITHGHEDHIGGLAYLLKQVNIPVYGTRLTIGLIEGKL
                     KEHGILKQCSLNVANPGDHI"
     gene            complement(860..>1332)
                     /locus_tag="ACZPE2_06480"
     CDS             complement(860..>1332)
                     /locus_tag="ACZPE2_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319531.1"
                     /translation="DAPAAINDMADGYEKRVLRYPVQAKTPVRDELKYLSSKQIFLRD
                     GLFMAVKILGIMRETGYSFPKLLSQIPEFFVKHKTVSFSSVPEDICRAFPNDTVYPSY
                     NGAVMYRSGGRVLLKPSENGKTIEIVTEALNYEVSCELCDDIENKLRDLGSVLN"
BASE COUNT          318 a          297 c          273 g          444 t
ORIGIN      
        1 tttatatgat cgcccggatt tgcaacatta aggctgcact gctttaaaat accgtgctct
       61 ttaagcttac cctcgataag gcctattgta aggcgtgttc cgtacacggg aatgttaacc
      121 tgctttaaaa gatacgcaag tccgcctata tggtcctcgt gaccgtgtgt tatgactata
      181 ccgcgtattt tgcttgcatt ctcggttaca taggtaaagt ccggtattac aaggtctaca
      241 ccgagcattt ccgtatccgg aaattcaagt ccgcagtcaa caagcatcat atcgttgccg
      301 cactcataga gcgtcatatt cttgccgact tcattgagtc cgccgagaaa tgcaatttta
      361 agcggcacat tattcttttt gccgcggcgc ttactctgac cctttgagtt gccggttgct
      421 ttggttgtct tgcgttcgtt tttcacaggc cttgaagtac cgtttttctg aacggatttt
      481 tgctgtgttt ttgaattttg attttttgtt ttttgcggat tttgtttttt ctgaccggcg
      541 gaatttttct tgccggaagt tggttttttt gctgttttgc ggttctcaag actgtttttt
      601 tcgttagaga gagcgttttg cttcgagttt ttgggtacag ttacctttcc gcggctgacc
      661 tgctttttag gctgatccga ctgcggaata agtctctctg tttttttctt taacattaaa
      721 tatctcctta ataaaaaatg aatatatgta tcctgctttt taaaacgtac aaaaagcagg
      781 taaaatccta atcaagatac ggcaccactc caccgcataa tcatggatta taatataata
      841 ctattttttg cctcggatgt caattgagaa cagagcctaa atctctcagt ttattttcaa
      901 tatcatcaca cagctcacac gacacctcat agttcagtgc ctctgtgact atctcgattg
      961 ttttgccgtt ttcagacggc ttcagcagca ctctgccgcc gctgcgatac attaccgccc
     1021 cgttataaga cggataaacg gtgtcatttg ggaaagctct gcaaatatcc tcgggaacag
     1081 aagaaaagct gacggttttg tgttttacaa agaattccgg tatctgtgaa agcagttttg
     1141 ggaaagaata tccggtctcg cgcattatgc cgagtatttt taccgccata aacagcccgt
     1201 cacgcaaaaa aatctgtttt gatgacagat atttgagttc atcgcgaaca ggcgtcttcg
     1261 cctgcacggg gtaccgcaaa acacgctttt catatccgtc tgccatatca tttatagctg
     1321 ccggagcatc at
//
LOCUS       DBMDVO010000456         1331 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47370_length_1331_cov_1.643902, whole genome shotgun sequence.
ACCESSION   DBMDVO010000456 DBMDVO010000000
VERSION     DBMDVO010000456.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47370_length_1331_cov_1.643902"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(165..791)
                     /gene="nth"
                     /locus_tag="ACZPE2_06485"
     CDS             complement(165..791)
                     /gene="nth"
                     /locus_tag="ACZPE2_06485"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1319532.1"
                     /translation="MNKKELALKCVEALKKEYPDAICSLTARTPLQLLIATRLSAQCT
                     DARVNLVTPALFESYPTLDAFCEADREDVEKFIHSCGFYHGKARDIIGMCRMIRSDFG
                     GEVPDNIDDLIKLPGVGRKTANLIVGDVYKKPAVVTDTHLIRISNRIGLVNTKDPKKV
                     EFELKKILPPDESNDFCHRVVLHGREVCTARRAMCDKCCLSEFCKKKI"
     gene            complement(784..1011)
                     /locus_tag="ACZPE2_06490"
     CDS             complement(784..1011)
                     /locus_tag="ACZPE2_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319533.1"
                     /translation="MAFCDRLRLLRQSKLPPLTQTDLANAIGVSQRKISFMETGVTEP
                     SLKDLSAICRYFSVSADYLLGLPDDMEYYRE"
BASE COUNT          397 a          306 c          265 g          363 t
ORIGIN      
        1 tcctcaactg taaaatcctc gcccggattg cgcagtatca agagcagcac cttaattgcg
       61 gcaggcgatg cgagctttaa atatttatct gtcagcacgg acggaaccgc aaaaacggag
      121 ccgtacagcg cagggtttac attaaacata tctatgcctt caaatcagat tttctttttg
      181 caaaattcgg aaagacaaca tttatcgcac atcgcacgtc ttgcggtaca gacctctctg
      241 ccgtgcaaaa caactctgtg gcaaaaatca ttgctctcat cgggcggaag tatttttttt
      301 aattcaaatt cgaccttttt cgggtccttt gtgtttacaa gccctatacg gttggatatt
      361 ctgataaggt gcgtatccgt gacaaccgcc ggttttttat atacgtcgcc gactatcaga
      421 tttgcggttt ttctgccgac tccgggaagc tttatgaggt cgtcgatatt atccggcacc
      481 tcgccgccga aatcggagcg gatcatacgg cacataccga ttatatcacg cgccttacca
      541 tgatagaaac cgcacgaatg tataaacttt tccacatcct ccctgtccgc ctcgcaaaaa
      601 gcgtcaaggg tgggataaga ttcaaataaa gcgggagtta ccaaattaac tctcgcgtcg
      661 gtacactgcg ccgaaaggcg agtggctatt aaaagctgaa gcggcgttct cgccgtaaga
      721 gaacaaatag cgtcgggata ctcttttttc agagcctcga cgcactttaa agcaagttct
      781 tttttattca cggtaatact ccatatcatc tggcaagccg agcaaatagt cggcggaaac
      841 actgaaatag cggcatatcg ccgaaagatc cttcagcgaa ggctcggtaa cgccggtttc
      901 cataaaggaa atcttacgct gcgaaacccc tatggcattt gcaaggtcag tctgcgtcag
      961 cggcggcaat ttagactgcc gcagcaatct cagcctgtca caaaaagcca tatactcatc
     1021 tcctacaata atttacaaac ccacacccat taaatgaact taattttagc acaaacctct
     1081 ttttgaggca aggagaatta agtaaaaatt taactaacaa atttgtgcaa gaatttgctt
     1141 gacacaaaat gttgtgtaac cgcatttata atggtcaaaa taaagaatta tatctattgt
     1201 attgccccaa aaattttgct ataatattac agaaaaaaat aatttcagag ctttttcgga
     1261 gatataatta tgaaaaaaag ttttacaaaa caactgataa gtctcatttt ggcggtctgt
     1321 tttacgcttg c
//
LOCUS       DBMDVO010000457         1331 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47386_length_1331_cov_1.290244, whole genome shotgun sequence.
ACCESSION   DBMDVO010000457 DBMDVO010000000
VERSION     DBMDVO010000457.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47386_length_1331_cov_1.290244"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="ACZPE2_06495"
     CDS             complement(<1..818)
                     /locus_tag="ACZPE2_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319534.1"
                     /translation="MNLKLFSLISSDDIPATKLQIKISGSLNAFTDDDVSFKRFSSLK
                     EMFPSLGKALADSELIVIAVDPKIYVRTKTLLTKALSLDIEENPTVKKALGRAAEIPE
                     SQKLLQSQMPKGAVPFLTRDGVNSGFVISKGHQQIMYLPLDDARLDTVLRNGVIPYLT
                     SGETIAVREEAYAPKNATVKPSVPPKRQEAPAPEKAAQTPPALPESVGEITSKTVNIL
                     RESGSRVAVNGNQNSEYVKSFGSVSPDFFEFFTFTPHVEDKGDFNVTDYTAQMA"
     gene            complement(861..>1331)
                     /locus_tag="ACZPE2_06500"
     CDS             complement(861..>1331)
                     /locus_tag="ACZPE2_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1319535.1"
                     /translation="VFAELDKYKKPDGFKESPIIEYDTLFKTYKFKIYAAFIAGSAPE
                     QDNGYLFYYNTPKFPTKENFDAFIAAIDERKLYSTGVSVDYTDKLITLSTCSYEFSNA
                     RLVVVGRLLRDGESENVDTSLVTANENPRYPQVWYDKKGQKNPFASAEKWQPAE"
BASE COUNT          306 a          303 c          344 g          378 t
ORIGIN      
        1 tttgccatct gtgcggtgta atcggtgaca ttgaagtcgc ccttgtcctc gacatgcggt
       61 gtaaatgtga agaactcgaa aaagtcgggt gaaacagagc cgaaggattt tacatattcg
      121 gaattttgat tgccgttcac cgcaacacgg ctgccgctct cgcgaagaat atttacggtt
      181 ttggaagtga tttcccctac actctcggga agcgcgggag gcgtctgcgc ggctttttcg
      241 ggagccggtg cctcttgcct tttcggcgga acagacggct ttaccgttgc gtttttcggg
      301 gcgtaagcct cctcgcggac agctatggtc tcgccgctcg tgagatacgg aataacaccg
      361 ttgcgaagca ccgtgtcaag gcgtgcgtcg tcaagaggca gatacattat ctgttgatgt
      421 cccttagaaa tgacaaagcc ggaatttact ccgtcgcggg ttaaaaacgg gacggcaccc
      481 ttcggcatct gcgattgaag cagcttttgc gactcgggaa tttccgcggc gcgtccgagt
      541 gcttttttga ccgtcggatt ttcctcaatg tcaagagaga gtgcctttgt aagcagcgtc
      601 tttgttctga catatatctt cgggtcaacg gcaataacta taagctcgga gtccgcgagt
      661 gctttgccga gcgacgggaa catctccttg agagaagaaa atctcttgaa ggacacgtca
      721 tcgtcggtaa aggcgttaag actgccggaa attttgattt gaagcttagt agccggaatg
      781 tcgtcactcg aaataagtga aaacaatttc aaattcatat ccttgtccat ccttgtttat
      841 aaatgtgtat taaaaaccga ttattcggcg ggctgccatt tttccgccga agcaaagggg
      901 ttcttttgac cttttttatc ataccatacc tgcggatagc gcggattttc gttggccgtg
      961 acgagcgaag tgtctacatt ttcgctttcg ccgtcgcgca gaagtctgcc gacaaccaca
     1021 agtcttgcgt ttgaaaattc gtagctgcag gtggatagtg taataagctt atccgtatag
     1081 tctacgctga cgcctgttga ataaagcttt ctctcgtcta ttgccgctat aaatgcgtca
     1141 aagttttcct ttgtcgggaa tttgggcgtg ttgtagtaaa agaggtagcc gttatcctgc
     1201 tcgggcgcgc tgcctgctat aaaggcggca tagattttaa atttataggt cttaaacaga
     1261 gtgtcgtatt ctataatcgg cgactcctta aagccgtcgg gcttcttgta tttgtcaagc
     1321 tcggcaaata c
//
LOCUS       DBMDVO010000458         1330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47436_length_1330_cov_1.624898, whole genome shotgun sequence.
ACCESSION   DBMDVO010000458 DBMDVO010000000
VERSION     DBMDVO010000458.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47436_length_1330_cov_1.624898"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="ACZPE2_06505"
     CDS             complement(<1..440)
                     /locus_tag="ACZPE2_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1319536.1"
                     /translation="MSEKDLEQFNADVQENEAATAAPVDNASPEAIGEAIKEINGEAD
                     ASAAPEKADDELQVGVIKPIATGKPESKRALKKAEKKKAKELKNIYHVSNWQIIKNYR
                     NYNQQERKHYKYIFRRRITEKVWPVFRFLILFGLGFVILYPILY"
     gene            complement(453..>1045)
                     /locus_tag="ACZPE2_06510"
     CDS             complement(453..>1045)
                     /locus_tag="ACZPE2_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1319537.1"
                     /translation="KFRGRTFARAVFFLPVLVATGPVLAVIKGDISTNGISSGEQFSA
                     LFQTDLVDELLQFLGIYNLNQQLTMTIQTITSDIFNLLWSAGIQILIFLAALQQIPVS
                     AKEAASMEGATGWEFFWKITFPMISPMILANLIYTVIDTFIDSENPVMSIVLAQSRGL
                     RYGLSAAMAWIYFLIVAVALAIIVAIVSKFVFYEND"
     assembly_gap    1046..1055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1056..1282)
                     /locus_tag="ACZPE2_06515"
     CDS             complement(<1056..1282)
                     /locus_tag="ACZPE2_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1319538.1"
                     /translation="MCYTELVTTADQAAQRGMTSAGIYTEWNNFGNYEEALFKNQDYL
                     PKLTESLGAMVPQVIVVMIFSLFIALLLNQK"
BASE COUNT          352 a          321 c          269 g          378 t
ORIGIN      
        1 atataaagta taggatagag aattacgaat cccagaccga acaggataag gaaacggaaa
       61 acaggccata ccttttcggt aattcttctt ctgaagatgt acttgtagtg ctttctttcc
      121 tgctggttgt aattacggta attcttgatt atctgccagt tgcttacatg gtagatattc
      181 ttaagctctt tagccttttt cttttcagcc ttcttcaaag ctctctttga ttcgggcttt
      241 cctgttgcga taggcttgat aacacctacc tgaagctcgt catcagcctt ttcgggagcg
      301 gcagaagcgt cagcctcgcc gttaatctcc tttatagcct cgccgatcgc ttccggagaa
      361 gcgttatcga caggtgcagc tgtggctgct tcgttttcct gaacatctgc attgaactgt
      421 tctaaatctt tttcgctcac aactacacct ccttaatcat tttcatagaa tacgaactta
      481 gatacaatag caacgataat tgcaagtgca accgcaacta tcaggaagta aatccatgcc
      541 atagccgccg aaagaccgta tcttaagcct cttgactgtg caagaactat tgacataacc
      601 gggttttccg aatcgatgaa cgtatcgata accgtataaa tcaggtttgc caatatcatc
      661 gggcttatca tcgggaacgt tatcttccag aagaattccc atcccgtagc gccctccata
      721 gatgccgctt cctttgccga aaccggtatc tgctgtaacg ccgcaaggaa tatcagtatc
      781 tgaataccgg cactccataa taagttgaaa atatcactcg ttatcgtctg tatcgtcatg
      841 gtcagctgtt ggttcagatt atatatacct aagaactgca acagctcatc gacaaggtcg
      901 gtctggaaca gtgcgctgaa ctgctcaccg ctcgaaatac cgttggtcga tatatcaccc
      961 ttgattaccg ccagtacagg acccgtcgca acaagtaccg gtaagaagaa taccgctctt
     1021 gcgaatgttc tgcctctgaa cttctnnnnn nnnnnaactt ctggttaaga agaagtgcga
     1081 taaacagcga gaatatcatt actacgataa cctgcggtac cattgcacca agtgactcgg
     1141 tcagcttggg gaggtaatcc tggttcttga aaagagcctc ctcatagttt ccgaagttgt
     1201 tccactcggt atagatacct gctgaagtca ttcctctctg tgcggcctga tctgctgttg
     1261 taacaagctc agtatagcac attgagtacc atgccgactt gaatatcggc acaatgaaca
     1321 tatagattac
//
LOCUS       DBMDVO010000459         1330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47461_length_1330_cov_0.884459, whole genome shotgun sequence.
ACCESSION   DBMDVO010000459 DBMDVO010000000
VERSION     DBMDVO010000459.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47461_length_1330_cov_0.884459"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="ACZPE2_06520"
     CDS             <1..492
                     /locus_tag="ACZPE2_06520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR1319539.1"
                     /translation="FGKAPAIATFICDVGKGIVAVVLAKKLLCGDADPLVCGYIAAVF
                     SILGHMFPIYFKFRGGKGVATALGVTTTLAWQTFPIVMIPFVIIVAVSKMISLGSIIS
                     AAILPIATYFVYYFWYPESSPVVPTVATAVIALLIIFMHRTNIKRMVRGEENKLGAKK
                     SDE"
     gene            complement(547..1218)
                     /locus_tag="ACZPE2_06525"
     CDS             complement(547..1218)
                     /locus_tag="ACZPE2_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319540.1"
                     /translation="MKKIVDFEICDIVPYSNGIIFAKKEMLDDNRCKVTFYGYDIKRA
                     KPIPVTKSVYQLNKFGNAFKAICDNIGDYISCDTAVMRNKDIAVVYPSGETGIFDKDG
                     NSKWSGDLFYHDSPVQGVAADGPLIWCTVPEQNAIINYSVTHKKFSLRIGGDSSTAFD
                     NPYSLSIYGNELFVCNAGSCKIRTINLKDFSVNDFRLFDEPIYRYLRVCGREIAVLES
                     GVYIL"
BASE COUNT          383 a          268 c          247 g          432 t
ORIGIN      
        1 ttcggaaaag ccccggcaat agctactttt atatgtgatg tcggaaaagg gatagttgct
       61 gttgttttgg caaaaaaact gctttgcgga gatgctgacc cactggtctg cggatatatt
      121 gctgccgtct tttcaatact cgggcatatg tttcctatct actttaaatt tagaggagga
      181 aagggtgtcg ctactgctct cggagttaca acaactctcg catggcaaac atttccgata
      241 gtgatgattc cgtttgttat tattgttgcc gtctctaaaa tgatttctct cggttcaatt
      301 atttcggcgg caatattacc gatagcgact tactttgtat attacttttg gtatcccgaa
      361 tcttctccgg ttgttccgac cgttgcaact gccgttatag ccttgctcat aatttttatg
      421 catcggacga atataaagcg aatggttcgc ggtgaagaaa acaagctcgg agccaagaag
      481 tcggacgaat aatgatttaa tataacaaaa agcctgttct taagaacagg ctttaactat
      541 taagatttac aatatgtaaa cgccggattc aagcacggct atttctctgc cgcaaactct
      601 gagataacga taaatcggtt cgtcaaaaag acgaaaatca tttaccgaaa aatctttaag
      661 gttgatagtt cttatcttgc aagagccggc attgcagaca aacaactcgt taccgtatat
      721 actgagcgaa tagggattat caaaagccgt tgaagaatct ccgccgatac gtaaactgaa
      781 ttttttgtgt gtaacagagt aatttataat tgcattttgc tccggtacgg tacaccaaat
      841 taaaggtccg tcggcagcga cgccttgaac aggggagtca tgataaaata aatccccgct
      901 ccatttactg ttgccgtcct tatcaaaaat tccggtttcg ccactcggat agacaactgc
      961 aatatcctta tttctcatta cggccgtgtc gcaggagatg taatcaccta tattatcgca
     1021 aatcgcctta aaagcattgc cgaatttatt aagctgataa acgctttttg tgaccggaat
     1081 cggttttgct ctttttatat catacccata aaacgtaacc ttgcatctgt tatcgtcaag
     1141 catttctttt ttagcaaaaa taattccgtt tgaatacgga actatatcgc agatttcaaa
     1201 atccactatt tttttcattt ttatatcacc gttatatgta aaaaactaat attatattta
     1261 attacataat ataatactcg tttttttccc ttcggtcaac cgcatttgac tatttttgcg
     1321 tagtttttga
//
LOCUS       DBMDVO010000460         1330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47470_length_1330_cov_0.764849, whole genome shotgun sequence.
ACCESSION   DBMDVO010000460 DBMDVO010000000
VERSION     DBMDVO010000460.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47470_length_1330_cov_0.764849"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            37..534
                     /locus_tag="ACZPE2_06530"
     CDS             37..534
                     /locus_tag="ACZPE2_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013286.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR1319541.1"
                     /translation="MSYFRANKATVFPNITDVKDYSTGIGDLPKSNVLKFFLKGGSWI
                     AVRPSGTEPKLKMCYSVRGIDSSTCERSLQNIRTIINGIMGMDIETYIKKIIRPKIQG
                     DGGEVEFESLSEDGTLTLIFRGECSKCLILNRCVDWIAEEVLKNTSKLVKIKAVRKKP
                     YFWDN"
     gene            546..902
                     /locus_tag="ACZPE2_06535"
     CDS             546..902
                     /locus_tag="ACZPE2_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319542.1"
                     /translation="MAHIKVVRRSMRPEEWNDLRFGWLKRYIINDKLPIDNIVIKDAR
                     QVTENNYEFYSDEFRPLKKGDLYFTPDGTAFIKAETEVPDSLKNKELWLYLKTAAEII
                     VKANGKFDEIVDKYTK"
BASE COUNT          455 a          218 c          281 g          376 t
ORIGIN      
        1 aaaggaaaag acggggccta gcgtataaag aatattatgt cttatttcag ggctaataaa
       61 gcaaccgttt ttccaaacat tacagatgtt aaggactata gcacgggcat cggcgattta
      121 ccgaaatcaa atgttttaaa gtttttcctt aaaggcggtt cttggattgc cgtcagacca
      181 tcgggaaccg agccgaaatt aaaaatgtgt tattccgtaa ggggtattga ctcttcaacg
      241 tgcgagcgtt cgcttcagaa tatccgaaca attataaacg gaataatggg tatggatatt
      301 gaaacatata taaaaaaaat aataagacct aaaattcagg gcgacggcgg tgaggttgaa
      361 tttgagtcat tgtctgagga cgggacttta actttgattt tcagaggaga atgttctaaa
      421 tgccttatct tgaacagatg cgttgactgg atagcggagg aagtgctgaa aaatacaagt
      481 aagcttgtaa aaataaaggc agttcgtaaa aaaccgtatt tttgggataa ctgagaggaa
      541 taattatggc acatattaaa gtcgtcaggc gcagtatgcg tcccgaagaa tggaatgatc
      601 tcagattcgg ttggctcaaa agatatatta taaatgacaa attaccgata gataatatag
      661 ttattaaaga tgcccgtcag gttacggaaa acaactatga gttttattcc gacgaattca
      721 gaccgctgaa aaaaggagac ttgtatttta ctccggacgg cacagcattt ataaaagcag
      781 aaacggaagt gcccgacagt ttaaaaaaca aagaactttg gctttacctc aaaacggctg
      841 ccgaaattat cgtcaaggct aacggtaagt tcgacgaaat cgttgacaaa tacacaaagt
      901 aatccggtaa aaatactttt tgtatgacat attaaccatg cctgcccatg aaatcagcgg
      961 gcatggtttt tatttaagtt acaaccgctg ctcacaaaac aatatgcgtt atcagtgaac
     1021 atttatattt aaataatatt tcatagaaat gttatttata aaaaaagtga ccggaatggg
     1081 caatgataag tattgctttg taaacagcgg taaaccttat ttaaacaatt aaccaaatca
     1141 aaaaacgaga aacggaggtg gcctgaatgg ctgctatttt aaaaacgtat tccggaatgc
     1201 tccttgaatc tttgggtcag acaatgctcc tgacactgct ttcgcttgcg tttgcatttg
     1261 ttatcggtct tatattcggt cttatgaatg taagtaagag taaagtgctt aattttatcg
     1321 gaacgcttta
//
LOCUS       DBMDVO010000461         1326 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47630_length_1326_cov_1.795918, whole genome shotgun sequence.
ACCESSION   DBMDVO010000461 DBMDVO010000000
VERSION     DBMDVO010000461.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47630_length_1326_cov_1.795918"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="rsmH"
                     /locus_tag="ACZPE2_06540"
     CDS             complement(<1..619)
                     /gene="rsmH"
                     /locus_tag="ACZPE2_06540"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1319543.1"
                     /translation="MDFEHIPVLFNETVNSLEIKPDGLYVDCTAGGGGHSHAVAQRLS
                     DKGRLIAIDRDPEAIEVLKARFASFPNVEIVHNTFDNISAILNGRKADGIMADLGVSS
                     HQIDTAERGFSFHMDAPLDMRMSKEGKSAADVVNTYSENELYRIIRDYGEERYAKSIA
                     KNIVKERENKRISTTFELCDIIKMSMPQRAMRDSHPARRTFQAIRI"
     gene            complement(632..1120)
                     /locus_tag="ACZPE2_06545"
     CDS             complement(632..1120)
                     /locus_tag="ACZPE2_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1319544.1"
                     /translation="MNKVADENVKEKTTETSAAQKRTESFGSYKEHTIDAKNRLFIPA
                     KMRNKLGSTFRVFCPIDGSDCLYIYSDEEWSKIAESVINTQNFDMQRLFFGGMVEVEP
                     DKQGRITIPTEFCDFAGLKKNVVVAGVGKRLEIWDADKYYKTMERARNIRQTFDYSQI
                     QF"
BASE COUNT          348 a          308 c          264 g          406 t
ORIGIN      
        1 ctatacggat cgcctgaaaa gttctgcgtg ccggatgcga gtctctcatt gcacgctgag
       61 gcattgacat tttaataata tcgcaaagct caaatgttgt tgaaattctt ttattttcac
      121 gttcttttac tatattttta gctatactct ttgcataccg ttcttcaccg tagtctctga
      181 ttatacgata aagctcattt tcggaataag tgttaacaac atccgcggca cttttgccct
      241 ctttgctcat acgcatatca agcggcgcgt ccatatgaaa cgaaaaaccg cgctcggcag
      301 tatctatctg atgagagctg acgccgagat ccgccataat tccgtcggct tttctgccgt
      361 ttaatattgc ggatatatta tcaaatgtat tgtgaactat ctcaacattc ggaaaagacg
      421 caaagcgcgc cttcaatacc tctattgcct cagggtcgcg gtcaatggct atgagcctgc
      481 ccttatcgga aagcctttga gccacagcgt gggaatgccc cccaccgccc gcggtacaat
      541 cgacgtaaag gccgtcgggc tttatttcga gtgaattgac agtttcgtta aataatacgg
      601 gaatgtgttc aaaatccatt ttaaatacct atcagaactg aatctgcgaa tagtcgaagg
      661 tctgacgaat gtttctcgca cgttccatgg tcttgtagta tttatctgcg tcccatattt
      721 caaggcgctt acctacgccg gcaacgacaa cattcttttt aagcccggca aagtcgcaaa
      781 attcggtagg aatggtaatc ctgccctgct tgtccggctc aacctcaacc ataccgccga
      841 aaaaaagacg ttgcatatca aaattctgag tattgataac agactcggct atcttgctcc
      901 actcttcgtc ggaatagatg taaaggcaat cgctgccgtc tatcgggcag aacactctga
      961 aggtagaacc gagcttatta cgcatcttgg caggaatgaa aaggcggttt ttggcgtcta
     1021 tggtgtgttc tttatagctg ccgaagcttt cggtacgctt ttgcgccgcc gatgtttcgg
     1081 tagttttttc tttaacattt tcgtcagcca ctttattcat tcctcctttc tgctccaaag
     1141 tgtgggtttt tactccccaa atatgggttt tttaaacatt tctcacctat tgactgtaat
     1201 tataaagcat actcacccat tttgcaataa ctaaagcaaa aaaaactttg ttaaatttaa
     1261 atgaattttc gacatgattt ttgggcataa aagaacggta ttcagcataa tagccgaata
     1321 ccgtat
//
LOCUS       DBMDVO010000462         1325 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_47693_length_1325_cov_1.245098, whole genome shotgun sequence.
ACCESSION   DBMDVO010000462 DBMDVO010000000
VERSION     DBMDVO010000462.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47693_length_1325_cov_1.245098"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(186..1061)
                     /locus_tag="ACZPE2_06550"
     CDS             complement(186..1061)
                     /locus_tag="ACZPE2_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1319545.1"
                     /translation="MKKETEMSAEKVVKQPKPKLMSIKLEKLHPFRNHPYRVIDDESM
                     TELTESIKQYGMLSRIIVSPLEDKPEEYEIISGHRRVHVAELLGMKEVPAVVHFIDRD
                     EATVMMVDSNCQRENLTLMEKARSYRMKADALFHQGQTCGQTGHKSRDNVSNVDSGRT
                     VQRLIRLTYLVPELQQFVDSGKMKMLPAYELSFLNEEAQRDIVDRIDETECFPSHAQA
                     RRMRKAFEEGKLNYDTVTEIMAEIKPNQVEKLKIPMDDIRKYVPKDFTPQQISELAIK
                     FFKQNYDRQHNRDAR"
BASE COUNT          312 a          295 c          277 g          441 t
ORIGIN      
        1 cggcaaatct gcgaaatcac ttgtgatata attcccgata cggttattca gcaaggtgtt
       61 tttactcggt tttttgtaat cgggcttttg aaatcttgac tcgactacat atttgacgcc
      121 gttaacggta tattctttcg gtgagccgcc gaagcctaca ataaacttat tcataagccc
      181 cctccttatc tcgcgtcgcg gttgtgctga cggtcgtagt tttgtttgaa aaactttatt
      241 gcaagctcgg aaatctgttg aggagtaaaa tctttcggca catatttgcg aatatcgtcc
      301 attggaattt tcagcttttc cacctgattc ggcttgatct ctgccataat ctcggtaacg
      361 gtatcgtaat ttaatttgcc ttcctcaaac gcttttcgca tacggcgggc ttgtgcgtgt
      421 gacggaaagc attcggtttc gtcaatacgg tcaacaatat ctctttgggc ctcttcatta
      481 aggaaggaaa gctcataagc cggaagcatt ttcattttgc cgctatcaac aaactgctgt
      541 aattccggaa caagataggt caggcgaata aggcgttgca cggtgcgccc gctgtcgaca
      601 tttgaaacat tatcacgcga cttgtggcca gtctgtccac aagtttgacc ctgatgaaaa
      661 agagcgtctg ctttcatacg gtaagaacga gccttttcca taagcgtcag gttttctctt
      721 tgacagtttg agtccaccat catcaccgtt gcctcgtcgc ggtcgataaa atgaacaacc
      781 gccgggactt ctttcattcc aagaagctcg gcgacgtgta ctcggcggtg tccggatatg
      841 atttcgtatt cctccggttt gtcttcaagc ggactaacaa taatgcggct taacattccg
      901 tattgcttta tgctttcggt cagctccgtc atggactcat catcaatcac cctgtacggg
      961 tgattgcgga atgggtgaag cttttcaagc ttaatgctca taagcttcgg tttaggttgc
     1021 tttactacct tttcggcaga catttcggtt tctttcttca ttataaaatc tccttaaatt
     1081 ttctttgtct gccgtttctt ttgtaaaaag aatgtataga aaaatatgct tgaaacggca
     1141 gtttggtttg tgtttcataa ttactgcctt tcaatgttgt cactcttggc cttcgccgta
     1201 gtgtgcttct gcaaataatc ctttgtaaat gatttcgcaa tcgtaattat tcagtgcttt
     1261 tgaaaacctg tgatatttca tctttattgc ttccgacaat ccgtatctgc tgaggcaaat
     1321 atgat
//
LOCUS       DBMDVO010000463         1318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48046_length_1318_cov_1.182416, whole genome shotgun sequence.
ACCESSION   DBMDVO010000463 DBMDVO010000000
VERSION     DBMDVO010000463.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48046_length_1318_cov_1.182416"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1318
                     /locus_tag="ACZPE2_06555"
     CDS             <1..>1318
                     /locus_tag="ACZPE2_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1319546.1"
                     /translation="KNGFRYEIHFKAGEPVSELQKEPYNKKDTGTRIRWKPDIKVFTD
                     IDIPLEYYQETILRQSVVNAGIKFVLRNQTGEKSFDKYEYCYENGIVDYIKEFVGDDA
                     FSSVQFWQGERKGRDRADLPEYKVKLNVALCFSNKKQLKEYYHNSSFLEHGGAPEKAV
                     KSAFVSQIDSYLKANSKYLKSDSKINFQDVEDCLVLVSSSFSTQTSYENQTKKAITNK
                     FIQEAMTDFLRHQLEVYFIENRIDAEKIAEQVLINMRSRVKAESTRLNIKKTLQSGNS
                     MTDRIEKFVDCRSKDVSEREIFIVEGDSALGACKQARDSAFQAVMPVRGKILNCLKAE
                     YPKIFKNDIITDLIKVLGCGVEVSSKANKNLSLFNMDNLRWSKVIICTDADVDGYQIR
                     TLILTMIYRLMPTLIKEGKVFIAESPLYEITCKDETWFCYTEKEKQ"
BASE COUNT          427 a          264 c          306 g          321 t
ORIGIN      
        1 acaaaaacgg atttcgctat gagatacatt ttaaggcggg cgagcccgtc agcgagcttc
       61 aaaaggaacc gtacaataaa aaagataccg ggacaagaat acgctggaaa ccggatatca
      121 aggtgttcac cgatattgat attcctcttg aatactatca ggagactata ttaaggcagt
      181 cggttgtaaa tgcgggaata aaattcgttc ttcgcaatca aaccggcgaa aagagcttcg
      241 ataaatacga atactgctac gaaaacggta tagtcgatta tataaaggaa tttgtcggcg
      301 acgatgcgtt ttcttccgtg cagttttggc agggtgagag aaagggcaga gacagagccg
      361 atttgccaga atacaaggtt aagctcaatg ttgcgctgtg tttttccaat aaaaaacagc
      421 ttaaggagta ttaccataat tccagctttc ttgaacacgg cggcgctccc gaaaaagctg
      481 taaaaagtgc gttcgtttcg cagattgaca gctatttaaa agcaaacagc aagtatttaa
      541 agagcgacag caagataaat ttccaggatg tggaggattg tcttgtgctt gtttcgtcat
      601 ccttttcaac tcagacgtca tacgaaaacc agaccaaaaa agctataacc aataaattca
      661 ttcaggaggc tatgacggat tttctccgcc atcagcttga agtgtatttt atagaaaaca
      721 gaatagacgc cgaaaaaatt gccgagcagg tgctcataaa tatgcgttca cgcgtcaagg
      781 ccgaatccac aaggctcaac attaaaaaga cgcttcaaag cggcaacagt atgactgaca
      841 gaatcgaaaa attcgttgac tgccgctcaa aggacgtttc ggagcgtgaa atattcatag
      901 tggagggcga ctcggctctc ggtgcctgca agcaggcgag agacagtgcc tttcaggcag
      961 ttatgccggt acgagggaaa atactcaact gtcttaaggc cgaatacccc aaaatattta
     1021 aaaacgacat tataacagac cttatcaaag tgctcggctg cggcgttgag gtaagctcca
     1081 aggcaaataa aaatctgtcg cttttcaata tggataatct tcgttggagc aaggtcataa
     1141 tttgtaccga tgccgacgtt gacggttatc agatacggac gctgatatta acgatgattt
     1201 atcgtcttat gcccacgctt ataaaagagg gtaaggtgtt tatagccgaa tctccgcttt
     1261 acgaaataac ttgcaaggac gagacctggt tttgctacac cgaaaaggaa aagcagga
//
LOCUS       DBMDVO010000464         1313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48290_length_1313_cov_1.404290, whole genome shotgun sequence.
ACCESSION   DBMDVO010000464 DBMDVO010000000
VERSION     DBMDVO010000464.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48290_length_1313_cov_1.404290"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="yqeK"
                     /locus_tag="ACZPE2_06560"
     CDS             <1..412
                     /gene="yqeK"
                     /locus_tag="ACZPE2_06560"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR1319547.1"
                     /translation="PKQEQLKIITDDGIILTPTEKSSTKTWHQLSGAVYIKKYLGVKD
                     EDIISAVRYHTTGRENMTLLEKVVYIADYISADRKYPGVERMREKAYRSLDEAMLEGL
                     QFTVIENVKKGFPIHEDSVKAYNFIAISYERKKL"
     gene            409..762
                     /gene="rsfS"
                     /locus_tag="ACZPE2_06565"
     CDS             409..762
                     /gene="rsfS"
                     /locus_tag="ACZPE2_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1319548.1"
                     /translation="MTTEELLKLTVETLDRKKGMDIKALKVTDLTVIADYFVIVTGTS
                     PTHIKALSDDLEDKLAEKGKNAKSVEGKATGWILLDYGTVIVHVFTKESRENFNLEKL
                     WGDAEEVDVSEWISE"
     gene            807..>1313
                     /locus_tag="ACZPE2_06570"
     CDS             807..>1313
                     /locus_tag="ACZPE2_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1319549.1"
                     /translation="MEYDFKHIESKWQKKWEEEGVFHAVDNSDKPKFYGLVEFPYPSG
                     AGMHVGHIKAYSGLEVVSRKRRLQGYNVLFPIGFDAYGLPTENYAIKTGIHPRKVTDM
                     NIKKFTSQLKRVGFSFDWTRVIDTTEEGYYKWTQWIFLKMFENGLAFRDKTLVNYCPS
                     CKVVLSNED"
BASE COUNT          417 a          259 c          332 g          305 t
ORIGIN      
        1 tccgaagcag gagcagttga aaataatcac cgatgatggt ataattctaa cgcctacgga
       61 gaaaagcagc acaaagacgt ggcatcagtt atcgggcgcc gtatacataa aaaaatatct
      121 cggtgtaaaa gatgaggata taatttcggc ggtgcggtat cacaccacgg gcagagaaaa
      181 tatgacactt ctcgaaaagg tggtctacat agcggactat atctcggcgg acagaaaata
      241 tccgggcgta gagcgtatgc gtgaaaaagc ataccgaagt cttgacgagg caatgcttga
      301 ggggcttcag ttcaccgtta tcgaaaatgt aaaaaaaggc ttccccattc atgaggacag
      361 cgttaaagca tataatttta tcgcaatttc ttacgaaagg aaaaagttat gacgacagag
      421 gaattactca aattgacggt cgaaactctc gacagaaaaa agggtatgga tataaaggct
      481 ctcaaggtaa cggaccttac cgttattgcc gactatttcg tgatagttac cggcacgtcg
      541 ccgactcata taaaggctct ctcggacgat cttgaggaca agctcgccga aaagggcaaa
      601 aacgcaaaga gcgttgaggg taaggctacg ggctggatac ttcttgatta cggcacggtt
      661 atagttcacg tatttacaaa ggaaagccgt gaaaacttca atctcgaaaa gctctggggc
      721 gacgccgagg aggttgacgt ttccgagtgg ataagcgaat aatgctttta gataaaaatt
      781 gactatactg caaaaaggtg aaataaatgg aatacgattt taagcatatt gaaagcaagt
      841 ggcagaaaaa atgggaagag gagggcgttt tccacgccgt tgacaattcc gataagccca
      901 aattttacgg acttgtcgaa tttccatatc cgagcggagc aggtatgcac gtaggtcaca
      961 tcaaggcgta ttcgggtctt gaggtggtat ctcgtaagag aaggcttcag ggctacaacg
     1021 tgcttttccc gataggcttt gacgcatacg gtctgccgac ggaaaactat gccattaaaa
     1081 cgggcataca tccgcgcaag gtcaccgata tgaatataaa gaagtttaca agtcagttaa
     1141 agcgcgtggg attttcgttc gactggacaa gagttataga cacaaccgaa gagggctact
     1201 acaagtggac ccagtggata ttcctcaaga tgtttgaaaa cggtcttgcg ttccgcgaca
     1261 agactctcgt aaactattgc ccctcctgca aggtagtttt gtcaaatgag gat
//
LOCUS       DBMDVO010000465         1313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48298_length_1313_cov_1.204620, whole genome shotgun sequence.
ACCESSION   DBMDVO010000465 DBMDVO010000000
VERSION     DBMDVO010000465.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48298_length_1313_cov_1.204620"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="ACZPE2_06575"
     CDS             complement(<1..850)
                     /locus_tag="ACZPE2_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR1319550.1"
                     /translation="MSEENKKIDSLKEKLFYTQKHASEIISDDETKKADAFCDGYMDF
                     LRTAKTEREATEYFVEKAEKLGFKPFVRGGKYKCGDKVYLNNRGKAVILCVMGKEPVE
                     NGIRLSAAHIDSPRLDLKPNPLYEDNELAYFKTHYYGGIKKYQWTAMPLSLHGVMVKK
                     DGSKVKVNIGEDDGDPMFVVTDLLPHLAAEQMKRTLSDGIRGEELNILIGSRPFSKDE
                     GSEKVKLNIISILNEKYGVTESDFLSAELEAVPALKAVDIGFDRSMIGAYGHDDRVCA
                     YPAAEAV"
     gene            complement(847..>1313)
                     /locus_tag="ACZPE2_06580"
     CDS             complement(847..>1313)
                     /locus_tag="ACZPE2_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013618.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR1319551.1"
                     /translation="YQDKISLYAETYGVPEDLLYGVIHTESGYDEKAKSHAGAIGLTQ
                     ITPETFLWLQTKTGENLPEEALYDADTSVKYCAVFYGLLLKEFGGDEKTAIAAYHAGR
                     GQVNAWLRDPDISPDGKTLVNIPESETKKYVEKVQRAVSIYDKLYKKELNKI"
BASE COUNT          280 a          338 c          300 g          395 t
ORIGIN      
        1 aaacagcctc ggcagccgga tatgcgcaaa ccctgtcgtc atgcccgtag gcacctatca
       61 tactgcggtc aaagcctata tccacagctt ttaacgcagg tacggcctca agctctgcac
      121 ttaaaaagtc gctctcggta acgccgtatt tttcattgag aatgctgatg atattgagct
      181 taactttttc gctgccctcg tcctttgaga acgggcggct gcctatgaga atattaagct
      241 cttcaccgcg gataccgtcg gaaagcgtgc gtttcatctg ctctgcggca agatgcggca
      301 gaagatcggt aacaacaaac atggggtcgc cgtcgtcctc gccgatgttt accttaacct
      361 tagagccgtc ttttttaacc ataactccgt gaagcgagag cggcatagcg gtccactgat
      421 atttttttat gccgccgtaa taatgggttt tgaaatatgc cagctcgttg tcttcgtaaa
      481 gcggatttgg ttttaaatca aggcgcggag agtcgatgtg tgccgcagaa agacggattc
      541 cgttttcaac aggctctttt cccattacgc aaagtataac cgccttgccg cggttgttta
      601 aatatacttt atcgccgcac ttgtattttc cgccgcggac aaacggttta aagccgagct
      661 tttccgcctt ttcgacaaaa tattcggttg cctcgcgctc ggtctttgca gtgcgcagaa
      721 agtccatgta gccgtcgcaa aatgcgtcgg cctttttcgt ctcgtcgtcg cttattatct
      781 ccgacgcgtg cttctgtgtg taaaagagct tctctttgag agaatcaatt tttttgtttt
      841 cttcgctcat attttattta actccttctt gtataattta tcatatatcg aaacagccct
      901 ttggaccttt tctacatatt tttttgtctc gctctcgggt atgttcacga gagttttacc
      961 gtcgggtgaa atgtcggggt cgcgaagcca cgcgtttacc tgaccgcgcc ctgcgtgata
     1021 tgccgcaatt gcggtttttt cgtcgccgcc gaactccttt aaaagcagcc cgtaaaatac
     1081 ggcgcagtat tttaccgacg tatccgcgtc atacaaagcc tcctccggca aattctcgcc
     1141 tgttttggtc tgaagccata agaacgtttc gggcgttatc tgcgtgagtc ctattgcacc
     1201 cgcgtgcgat ttagcctttt cgtcatatcc gctctccgtg tggataactc cgtagagcag
     1261 atcctcgggg acgccgtagg tttcggcata cagcgatatt ttatcctgat act
//
LOCUS       DBMDVO010000466         1308 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48511_length_1308_cov_1.504557, whole genome shotgun sequence.
ACCESSION   DBMDVO010000466 DBMDVO010000000
VERSION     DBMDVO010000466.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48511_length_1308_cov_1.504557"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(64..>1308)
                     /locus_tag="ACZPE2_06585"
     CDS             complement(64..>1308)
                     /locus_tag="ACZPE2_06585"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1319552.1"
                     /translation="KFGLESAMEDYLRGTNGIMTTTTASDGTKTSEITREPVDGDTVI
                     LTLDSVLQKKVQDSLAAFVERYRDKDAIPAVGSAVVMDVNTGAVLACATYPSYDLNTY
                     YQNYEALSKDKSSPLWNRALMSTYEPGSTMKPAIAAAGLEEGVITETSKFYCSHIYRQ
                     FTDTTFKCLGSHGWIDVKNALNQSCNIYFYETGRLLGINRMNDYCTRFGLGQKTGVEI
                     NESSGVLAGIAYREAHGGTWYPGDTVQAAIGQSDNLFTPIQLCSYVSTIANGGTRYRA
                     HFVKSVKSSDYSETLLSNDGVVLNETGVSQNSIRIVKEGMEMLGARLPAFKSLPVKVA
                     AKTGTAESKAKVSGKIVTGLNGFMISFAPADDPQIAVCVAIENLNSGSATASLVSEIY
                     KAYFETDGGSVNTAQGYGSLLG"
BASE COUNT          267 a          340 c          344 g          357 t
ORIGIN      
        1 tcccggagcc gcccttgccg gagcacacta tatacttttt agccatcgga aatacccctt
       61 tcttcagccg agcagcgagc cgtagccttg cgcggtattg acgctgccgc cgtcggtctc
      121 aaagtaagcc ttgtatattt ctgagacgag cgaagctgtg gcggaaccgc tgttgaggtt
      181 ttcgattgcc acgcagaccg ctatctgagg gtcgtcggca ggcgcaaagg aaatcataaa
      241 gccgttaaga ccggttacga ttttgccgct cacctttgcc ttgctctctg cggtaccggt
      301 tttcgccgcc acctttacgg ggagactttt aaatgccgga agccttgcac cgagcatttc
      361 cataccctct ttgactatgc gtatgctgtt ttgcgaaaca ccggtttcgt ttaaaacaac
      421 gccgtcgttt gagagcagag tttcggaata gtcgcttgat ttcacgcttt ttacaaagtg
      481 cgcgcggtat ctcgttccgc cgtttgcaat ggtcgaaaca tagctgcaaa gctgtatggg
      541 cgtaaagagg ttgtccgact gacctattgc agcctgaacg gtgtcgcccg ggtaccatgt
      601 gccgccgtga gcctctctgt aagcaatgcc ggcaagtacg cccgaggatt cgtttatttc
      661 aacgcccgtc ttttggccga gtccgaatct tgtgcagtaa tcgttcatac ggtttatgcc
      721 caaaagcctg cccgtttcgt agaaatagat gttgcaggat tggttaagcg cgtttttaac
      781 gtctatccaa ccgtgtgagc cgagacattt gaaggtcgtg tcggtaaact gacggtaaat
      841 gtggctgcaa taaaattttg acgtttcggt tataacgccc tcctcgagcc ccgctgccgc
      901 tattgccggc ttcattgtgg aaccgggctc ataggtgctc ataagagctc tgttccaaag
      961 cggagagctt ttatcctttg agagagcctc gtaattttga tagtatgtgt tcaggtcata
     1021 gctcggatat gtcgcgcagg cgagcacggc accggtgttt acatccatta ccacggccga
     1081 accgaccgcc ggaattgcgt ccttgtcgcg gtacctttcg acaaatgccg caagcgaatc
     1141 ctgaaccttt ttttgaagca cggagtcaag cgtgagtatt accgtgtcgc cgtcaacggg
     1201 ctcgcgggtg atctcgctcg tttttgtgcc gtcggacgct gtggttgttg tcattatgcc
     1261 gtttgttccg cgaaggtaat cctccatggc actttcaagg ccgaattt
//
LOCUS       DBMDVO010000467         1304 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48756_length_1304_cov_0.740648, whole genome shotgun sequence.
ACCESSION   DBMDVO010000467 DBMDVO010000000
VERSION     DBMDVO010000467.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48756_length_1304_cov_0.740648"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..354
                     /locus_tag="ACZPE2_06590"
                     /pseudo
     CDS             136..354
                     /locus_tag="ACZPE2_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358252.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            503..>1304
                     /locus_tag="ACZPE2_06595"
     CDS             503..>1304
                     /locus_tag="ACZPE2_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HIR1319553.1"
                     /translation="MSNIRIFVSHRIDIDSETVDTPVYFPVRCGAVYDEEGNSEIQGD
                     DTGDNISKLRMSFCEFTVQYWAWKNVDADYYGLCHYRRYLSFSDKRYKNTVFNMVREL
                     KLTPHEKKKYHLDDTKKIEKTVKQYDMIIPVVAKASEMSYNRAEVKTLREMWESYNGV
                     YFEQGIIDKMFSLIKEYSPEYYNSACEYFSGKYHRAFNCYIMKKELFVRMCEFQFPIM
                     KRITEITDCKTYERAPGYIGEILNGIFIHHMLTVENRSAKETQLVFFVN"
BASE COUNT          444 a          213 c          289 g          358 t
ORIGIN      
        1 tgctgattat aactattgaa attcgcagaa taaagcgata taggcataca gcaaatgagt
       61 tttatataaa gaaggttatt tctaggcaac aaaccgcact tttttgaaaa aacatacttt
      121 ttctataaac tgaagatgga tcagataggc aacaacaacg atgacagaaa ccggctcgat
      181 gaaatagctt tgaaagaggc aataatcgga cttgacgaca gagagaagaa tattctcaag
      241 ctgcgttttt tcagaggaaa aactcaggta gaagtcgcac aggaaatagg aattagtcag
      301 gcacaggtgt cacggcttga aaaaggcgct ccgggaaaaa ttaaaaaagc actgcagcaa
      361 aaacgtgtca atctgcgaag gttgacacgt ttttttcttt gccttattac aatttgtctt
      421 ttttgttgac aaaaaaattg cactgtacta taattaaata ataggttgga ttggtgataa
      481 aacatttaag gcaggggaaa agatgagtaa tatccgtatt tttgtatcgc acaggataga
      541 tattgacagt gaaacagtag acactcccgt ttattttccg gtcagatgcg gtgcggttta
      601 tgatgaagaa ggcaacagtg agattcaggg cgatgacacg ggcgacaata tttcaaagct
      661 ccgtatgtca ttttgtgaat ttacggttca gtattgggcg tggaagaatg ttgatgccga
      721 ttattacgga ctgtgtcatt accggcggta tctttctttt tcggataaac gctataaaaa
      781 tacggtgttc aatatggtgc gtgagttaaa attgacacct cacgaaaaga aaaaatatca
      841 tcttgatgat acgaaaaaaa tcgaaaaaac tgtaaaacaa tatgatatga taattcccgt
      901 ggttgcaaaa gcgtccgaaa tgagttataa cagggcagag gtcaaaacgc ttcgtgaaat
      961 gtgggaaagc tataacggcg tgtattttga gcaggggata atagacaaga tgttttccct
     1021 tataaaagag tattcgcccg aatattacaa ctctgcctgc gaatactttt ccggaaaata
     1081 tcacagggcg ttcaattgct atataatgaa aaaagagctg tttgtccgta tgtgcgagtt
     1141 ccagtttcct attatgaaaa gaattacgga aatcaccgat tgcaaaacct atgaacgtgc
     1201 tcccgggtac ataggcgaaa tactaaacgg tattttcatc caccatatgc tgacggttga
     1261 aaacaggtcg gccaaggaaa cacagcttgt gttttttgta aata
//
LOCUS       DBMDVO010000468         1303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48778_length_1303_cov_1.393511, whole genome shotgun sequence.
ACCESSION   DBMDVO010000468 DBMDVO010000000
VERSION     DBMDVO010000468.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48778_length_1303_cov_1.393511"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            90..872
                     /locus_tag="ACZPE2_06600"
     CDS             90..872
                     /locus_tag="ACZPE2_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319554.1"
                     /translation="MKKIIEFFNSPKISRMGEYMLTGRYIMVLFALASVFVIFDISVA
                     GVLTFACITGITLVLCEDLLAPFPPFLFLCLIGTKCYNSFSVFIQYKALGVVLIICVI
                     MHFVLHWKKPVLKGFLTLPMIFVSAAVMLGGVGFISKREYFSGASIFYILALGVGMLL
                     LYTVFNTHINVHKDYSLMDKLSLIMVIIGCFGTFMVVSYYLTHINEVIDTKTILYFQW
                     RNNCSTFLMLSIPFAFYRGNKKSYSIMFGFLFYFAILLTGSR"
     gene            900..>1303
                     /locus_tag="ACZPE2_06605"
     CDS             900..>1303
                     /locus_tag="ACZPE2_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1319555.1"
                     /translation="MMCCILFFLYDRERRFAYIAILACICFALMIFSREFLSFFGYTF
                     DRLMSAINGVLVGEQKEVRVFQYERGIKDFLGHPIFGTGIGYMGNIDIYDPADFSICW
                     YHCEPIQIAASMGIVGILAYVYQFIKRMFLLW"
BASE COUNT          309 a          238 c          303 g          453 t
ORIGIN      
        1 ataatagtta tagtcttgaa ataattattg cgttttggca aaaaaaatat ataatatcat
       61 taaactataa aatgaaaagg tatgcgcaaa tgaaaaaaat cattgaattt tttaattcgc
      121 ccaagatttc tcgcatgggc gagtatatgc ttaccgggcg atatataatg gtgcttttcg
      181 cgctggcgtc ggtgtttgtt atatttgaca tatccgttgc cggtgtgctc acctttgcgt
      241 gcattacggg tataacgctt gttctgtgtg aggacctatt ggcgccgttt ccgccttttt
      301 tgtttctgtg ccttatagga acaaagtgct ataattcatt cagcgtgttt attcagtata
      361 aggcactcgg tgtggtactg ataatttgtg taattatgca ctttgttttg cattggaaga
      421 agccggtgct gaaaggattc ctcacgctgc cgatgatatt tgtgtctgct gcggtaatgc
      481 tcggcggagt cggtttcatc tcgaaacgtg agtatttcag cggcgcgtcg atattttaca
      541 ttttggcgct cggcgtggga atgctgttgc tctataccgt atttaatacg catataaatg
      601 tccataagga ttattctctt atggataagc tgtcccttat tatggtgata ataggctgct
      661 tcggtacctt tatggtagtc tcatattatc tcacgcatat aaatgaggtt atagacacaa
      721 aaacgatttt gtattttcag tggcgcaata actgctctac gtttttgatg ttgtcgatac
      781 cgtttgcatt ttatcgcggc aacaaaaaat catattccat tatgttcgga tttttgttct
      841 attttgcgat attgctgacc ggttcgcgct gaggacttgt tttcggtgcc atagagctta
      901 tgatgtgctg tattctgttc ttcctttacg acagggagag gcgttttgcg tatattgcca
      961 ttctcgcttg catttgcttt gcgcttatga tattctcgcg cgagtttctc tcgttcttcg
     1021 gctatacctt tgaccgcctt atgtctgcga taaacggggt gctcgtcggc gaacaaaagg
     1081 aagtgagagt ttttcagtac gagcgcggca taaaggattt tcttggccat ccgatattcg
     1141 gaaccggcat aggatacatg gggaatatcg atatatatga tcctgccgat ttctcgatat
     1201 gctggtatca ttgcgagcct atacagatag ccgcaagtat gggcatagtc ggaattttgg
     1261 cgtatgtcta tcaatttata aagagaatgt ttctgctttg gag
//
LOCUS       DBMDVO010000469         1302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48838_length_1302_cov_1.279767, whole genome shotgun sequence.
ACCESSION   DBMDVO010000469 DBMDVO010000000
VERSION     DBMDVO010000469.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48838_length_1302_cov_1.279767"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="ACZPE2_06610"
     CDS             complement(<1..778)
                     /locus_tag="ACZPE2_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HIR1319556.1"
                     /translation="MLEFRNPELSDRAWVTGVMERSGDMACEYCFGNLYIWAPVYENT
                     ICEYDGMFLARDGIGDPMYLYPCGDGDKKAAVNELIRYAENHDGARLTMYCLTPSNVR
                     ELEDMMPGKFEFTEMREFADYIYNTTDLIDLAGRKYHGKRNHIAYFKNNHDWHFEPIN
                     DGNMQLCLDMNRKWEALNREKDPDEIDSELEAIKRAFSNFDELGFKGGVLYADGEVVA
                     YTLGEELNSSAFCTHIEKAYASVRGAYPTVNREFAANMLAN"
     gene            complement(781..>1302)
                     /locus_tag="ACZPE2_06615"
     CDS             complement(781..>1302)
                     /locus_tag="ACZPE2_06615"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1319557.1"
                     /translation="YLGGEFGNDPSCVNVYAASSFYAVDRYASYSRHWKEDYLSGKVI
                     LADRYTSSNAVHQAVKLPQSEWDDYIKWLCDYEYEKLAVPKPDRIIYLDMPVDVSQRL
                     MTKRYGGDEVKKDVHEANVEYLRRCAKAAAFAAEKMGWETVVCSENGEPRPIADISEE
                     ILGIVKKELGLGE"
BASE COUNT          310 a          359 c          280 g          353 t
ORIGIN      
        1 aattagcaag catatttgcc gcaaattcac ggtttaccgt cgggtaagct ccgcgaaccg
       61 aagcatacgc cttttctata tgtgtgcaaa aagccgagct gttcagctcc tcgccgagag
      121 tgtaagccac aacctcgccg tccgcgtata aaactccgcc tttgaacccg agttcatcaa
      181 aattcgagaa cgcacgcttt atggcttcaa gttcgctatc tatttcatcg gggtcctttt
      241 cacggtttaa agcctcccac ttccggttca tatcaagaca taattgcata ttgccgtcgt
      301 ttatcggctc aaaatgccaa tcgtggttat tcttgaaata agctatatgg ttacgcttgc
      361 cgtggtactt cctgccggca aggtcaataa gatcggtggt attgtaaatg taatcggcga
      421 attccctcat ctcggtaaac tcaaactttc cgggcatcat atcttcaagc tcacggacat
      481 ttgacggagt gaggcagtac atggttaatc gcgcgccgtc gtgattttcg gcataacgta
      541 taagttcgtt tacggcggcc tttttatcgc cgtcgccgca cgggtaaaga tacatcggat
      601 cgcctatgcc gtcgcgcgcc aaaaacatac cgtcatactc gcaaattgta ttttcataca
      661 cgggcgccca tatataaagg ttaccgaagc agtactcgca ggccatatcg cccgaacgct
      721 ccattacgcc cgttacccaa gctctgtccg ataattcggg gtttctgaat tcaagcaaaa
      781 ttattctcct aaaccaagtt ctttttttac tatacccaat atttcttcgc ttatatcggc
      841 tatggggcgc ggctcaccgt tttcggagca gaccacggtc tcccagccca ttttttccgc
      901 ggcaaatgcc gccgcttttg cacagcgacg caaatactct acatttgcct cgtggacgtc
      961 ttttttcacc tcgtcgccgc cgtagcgctt tgtcataagt cgctgtgaca cgtcaaccgg
     1021 catatcaaga tatataatcc tgtcgggctt cggcacggcg agcttttcgt attcgtaatc
     1081 gcaaagccat ttaatatagt cgtcccactc gctctgcggc agctttaccg cctgatgaac
     1141 ggcgttcgac gaagtatatc tgtcggctaa aatgacctta cccgaaagat aatcctcttt
     1201 ccaatgacgg ctgtagctcg catatcggtc caccgcataa aacgaggacg ccgcatagac
     1261 gttgacgcac gacgggtcat ttccgaattc gccgccgaga ta
//
LOCUS       DBMDVO010000470         1300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48929_length_1300_cov_1.420350, whole genome shotgun sequence.
ACCESSION   DBMDVO010000470 DBMDVO010000000
VERSION     DBMDVO010000470.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48929_length_1300_cov_1.420350"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>632)
                     /locus_tag="ACZPE2_06620"
     CDS             complement(<1..>632)
                     /locus_tag="ACZPE2_06620"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1319558.1"
                     /translation="VTGVAWGLYGKVNYAIEGSAFNAGSVIKWLRDDLRLIDSARRCD
                     ELAESVDDANGIYFVPAFTGLGAPYWDMYARGTIVGLTRGVKAEHIARSVLEAIAFQM
                     TDLLEAMKADSGIKLSELRVDGGASVSNIMMQIQANLIRTKVNRPKVVETTALGAAYL
                     AGLAVGFWKDLDEIEAIREVDKIFEPQMPLVKRDEIYHGWLRAVERSRDW"
     assembly_gap    633..642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<643..>1300)
                     /locus_tag="ACZPE2_06625"
     CDS             complement(<643..>1300)
                     /locus_tag="ACZPE2_06625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1319559.1"
                     /translation="KKVISSDIAAIGITNQRETTILWDKGTGKPIYNAIVWQCRRTAE
                     MCEEIKKDKELCDYIKEHTGLVVDAYFSATKIKWVLENVPGARILADAGQLLFGTVET
                     WLIWNLTGGQVHVTDYSNASRTMLFDVDKLEWDPYLCEKLDIPMSILPEVKPSSEVYG
                     TVAPGLLGFEDIVGTPISGAIGDQPAALFGQGCFKTGQAKNTYGTGCFLLMNTGENRV
                     "
BASE COUNT          305 a          384 c          269 g          332 t
ORIGIN      
        1 atccaatctc tggagcgctc tacggctctg agccatccgt gataaatctc gtcacgtttt
       61 acaagcggca tctgaggctc aaatattttg tctacctctc ttatggcctc aatttcgtcg
      121 agatccttcc aaaagcctac cgcgagtccg gcgagatatg ccgctccgag agccgtagtc
      181 tcaacgacct taggcctgtt gacctttgtt cttatcaggt ttgcctgtat ctgcatcatt
      241 atattgctga cagacgcgcc gccgtcaacg cgaagctcgg acagcttaat tcccgaatcc
      301 gccttcatgg cttcaagcag gtctgtcatc tgaaaagcta ttgcctcaag aacgcttctt
      361 gcaatatgct ccgccttaac gcctcttgta agccccacaa tcgtgccgcg cgcatacata
      421 tcccaatacg gagcgccgag tcccgtaaac gccggaacaa aataaatgcc gtttgcatcg
      481 tcaacactct ccgcaagctc atcgcaacgg cgtgccgaat caatgagacg caaatcgtct
      541 ctcagccatt taataaccga gcccgcatta aatgccgagc cttcaatcgc ataattgacc
      601 ttaccgtaaa ggccccacgc cacgccggtt acnnnnnnnn nnttcaccct gttttcgccc
      661 gtattcataa gcaaaaagca gcctgtgccg tatgtatttt ttgcctgacc cgtcttaaag
      721 catccctggc cgaacagagc ggcaggctga tcgcctattg cgccggaaat cggagttccg
      781 actatatcct caaagccgag aagcccggga gctaccgttc cgtacacctc gctcgacggc
      841 ttaacctccg gcaaaattga catcggaatg tcaagctttt cgcagagata agggtcccac
      901 tcaagcttat caacgtcaaa aagcattgtt cttgacgcat tggaatagtc cgtaacgtga
      961 acctgacctc cggtgagatt ccatataagc cacgtctcta cggttccgaa aagcaactgc
     1021 ccggcatcgg caaggattct tgctccgggg acgttttcca gcacccattt gattttggtg
     1081 gcagagaaat aagcgtccac aacaagccct gtatgctcct ttatgtagtc acaaagctcc
     1141 ttgtcttttt ttatttcctc gcacatctcc gccgtcctgc ggcactgcca tactatcgca
     1201 ttgtaaatcg gcttgcccgt acccttatcc caaagaatgg tggtttcgcg ctgatttgta
     1261 attcctatcg cggctatatc cgatgaaatc accttctttt
//
LOCUS       DBMDVO010000471         1300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48932_length_1300_cov_1.305254, whole genome shotgun sequence.
ACCESSION   DBMDVO010000471 DBMDVO010000000
VERSION     DBMDVO010000471.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48932_length_1300_cov_1.305254"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1300)
                     /gene="argS"
                     /locus_tag="ACZPE2_06630"
     CDS             complement(<1..>1300)
                     /gene="argS"
                     /locus_tag="ACZPE2_06630"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1319560.1"
                     /translation="VLPAEPIPGFKVEVPADRANGDYSVNAAFACARAFRTAPKKIAD
                     AVSKYIDLGDSYFESCSVAGPGFINFTLGDRYYADILLDIEECGEKYGSSDFGKNKKV
                     NVEFVSANPTGPMHMGNARGGALGDCLASVLSMAGYDVSREFYINDAGNQIDKFALSL
                     DIRYQQLFLGDKAPSLPEDSYHGADIKERAEEFAKQYGDSYMEKSEEQRRKALVDFAL
                     PKNIQNMKDNLAKYRIKYDTWFHESVLHNGAVDDVIKILTDNGMTYEKDGALWYKNIE
                     MQTKLLKKAGKTKEQIDRLELKDDVLIRQNGNPTYFAADIAYHRNKLVDRGFDKAIDV
                     WGADHHGHVARLKGALEAIGIDSSRLDVVLMQLVNLMKDGEPVRMSKRTGNAVTLVDL
                     LDEVPIDAARFFFNMREPGSTIDFDLGLAVKEDSQNPVYYC"
BASE COUNT          273 a          327 c          330 g          370 t
ORIGIN      
        1 ggcagtaata aacggggttt tgcgaatcct cttttacggc aagtcccaaa tcgaagtcta
       61 ttgttgagcc gggctcgcgc atattgaaga aaaatcttgc ggcgtcaatc ggtacttcgt
      121 caagcaaatc gacaagcgtt acggcgtttc ccgtgcgctt tgacattctt acgggttcgc
      181 cgtccttcat aaggtttaca agctgcatta aaacaacgtc gagtcttgac gaatcaatgc
      241 ctatggcttc aagtgcgccc ttgagccttg ccacgtggcc gtggtggtcg gcgccccaga
      301 cgtctatcgc tttgtcaaag ccgcggtcaa caagcttgtt gcggtgatag gctatgtcgg
      361 cggcaaagta tgtgggattg ccgttttgac gaatgagcac atcgtccttt aattcaaggc
      421 ggtctatctg ctccttcgtt ttgcccgcct ttttaagcag ctttgtctgc atttcgatgt
      481 ttttatacca gagagcaccg tctttttcat atgtcatacc gttgtcggtg aggattttta
      541 tcacgtcgtc gacagcaccg ttatgaagca cgctctcgtg gaaccatgtg tcgtatttaa
      601 tgcggtattt ggcgagattg tccttcatat tctgtatgtt tttaggcaaa gcgaagtcta
      661 cgagtgcttt tctcctctgt tcctcgctct tttccatata gctgtcgccg tactgctttg
      721 caaattcctc ggcacgctcc tttatgtccg caccgtgata gctgtcctcc ggaagagagg
      781 gcgctttatc gcccaaaaac agctgttgat aacgaatatc caaggagagt gcaaatttat
      841 ctatctgatt gcctgcatcg ttgatataga actcgcgcga gacgtcgtat cctgccattg
      901 acagtaccga ggcgagacag tcgccgagag caccgccgcg cgcgttgccc atatgcatgg
      961 gacccgtggg atttgccgag acgaattcga cgtttacttt tttattcttg ccaaagtcgc
     1021 tgctgccgta tttttcgccg cactcctcta tgtcgagcag tatatcggcg taatatctgt
     1081 cgccgagcgt gaagtttata aatccgggac ctgcaacgct gcaggactcg aaatatgagt
     1141 cgccgaggtc aatatatttc gaaacggcgt cggcgatttt tttaggagcg gtgcgaaatg
     1201 ctctcgcgca ggcaaacgcc gcgtttaccg agtagtcgcc gttggctctg tcggccggga
     1261 cctctacctt gaagcccggt ataggttcgg cgggtaaaac
//
LOCUS       DBMDVO010000472         1300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_48952_length_1300_cov_1.050042, whole genome shotgun sequence.
ACCESSION   DBMDVO010000472 DBMDVO010000000
VERSION     DBMDVO010000472.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48952_length_1300_cov_1.050042"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(52..864)
                     /locus_tag="ACZPE2_06635"
     CDS             complement(52..864)
                     /locus_tag="ACZPE2_06635"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1319561.1"
                     /translation="MADIIERNAEYIRSKITEIPKTAIVLGSGLGDMGEKIENPVYID
                     YGELEGFAVSTAPGHKGRFIVGRLSGKPVICMQGRLHCYEGWELDKVVLPIRVMRALG
                     AENLILTNAAGGVNLDFKPGDIMLITDHINFMGRNPLVGKNDDSVGTRFPDMSFAYNP
                     SLREIAEACAAKTGTDLKKGVYLGCLGPSYETPAEIRAFRILGADAVGMSTVPEVIAA
                     NHCGFKVLAFSLITNMAAGVLKQPLSEEEVLTTGKLKAREMQKLISEILLNL"
     gene            complement(857..>1300)
                     /locus_tag="ACZPE2_06640"
     CDS             complement(857..>1300)
                     /locus_tag="ACZPE2_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1319562.1"
                     /translation="KSGVNVAIGTDSVASNNNLNMIKEMFLCALLPKGLHNDASVVSE
                     RDILKIATVNGYKAQGREDGGAIKAGYKADLCVLNIESEHMYPQTDMINNLVYAADGA
                     DVVLTMADGRVLYKDGEFTTIDIEKVKSTVNAAASRIISEVKKNG"
BASE COUNT          303 a          333 c          276 g          388 t
ORIGIN      
        1 atgttttttt gctttataac gagctttcgg aagaagattt gcaccgtaaa atcacaaatt
       61 cagaagtatc tccgaaatga gcttttgcat ttcgcgagcc ttgagtttac ccgtggtcag
      121 cacctcttcc tcgcttaacg gctgttttaa aactcctgcc gccatatttg tgataagaga
      181 aaaagcgagc actttaaatc cgcaatgatt ggcggctatt acctccggaa cggtggacat
      241 accgactgcg tccgctccga gtatacgaaa tgcacgaatt tccgccggtg tttcatacga
      301 cggcccgagg cagccgagat atacgccttt tttaaggtcg gtacccgttt ttgcggcgca
      361 tgcttcggca atttcacgaa gtgacggatt atatgcaaac gacatatccg ggaaacgtgt
      421 gcctacgctg tcgtcatttt ttcccacgag cggatttctg cccataaaat tgatatggtc
      481 ggttataagc ataatatccc cgggtttaaa gtcgagatta acaccgccgg cggcattggt
      541 aagtatcaga ttttccgctc cgagggcacg cataactctt ataggcaata cgactttatc
      601 aagctcccaa ccctcatagc agtgaagcct gccctgcata caaataacgg gtttccccga
      661 aagcctgcct acgataaacc tgcccttgtg acctggagcg gtagatacgg caaagccttc
      721 aagctcgccg taatctatat acacggggtt ttcgattttt tcgcccatat cgccgagccc
      781 cgagcccaaa actatggctg ttttcggtat ttcggttatt tttgacctta tatattctgc
      841 gtttcgctct atgatgtcag ccatttttct ttacctcgct tataattctg ctcgctgcgg
      901 catttacggt gcttttgacc ttttcaatat caatcgttgt gaattccccg tccttataaa
      961 gcactcttcc gtccgccata gtcagcacaa catcggcacc gtctgcggca tacacaaggt
     1021 tatttatcat atcggtttgc ggatacatat gctcgctttc gatgtttaaa acgcaaaggt
     1081 cagctttgta tcccgctttt attgcgccgc cgtcctctct gccctgtgcc ttgtaaccgt
     1141 ttacggtagc tatttttaaa atatcgcgtt cggaaaccac cgaggcgtcg ttatgaagtc
     1201 ctttcggcaa aagcgcgcaa aggaacattt cctttatcat attaagattg ttgtttgagg
     1261 cgacgctgtc tgtacctatt gccacattga cgccgctttt
//
LOCUS       DBMDVO010000473         1297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49094_length_1297_cov_1.277592, whole genome shotgun sequence.
ACCESSION   DBMDVO010000473 DBMDVO010000000
VERSION     DBMDVO010000473.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49094_length_1297_cov_1.277592"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="ACZPE2_06645"
     CDS             complement(<1..858)
                     /locus_tag="ACZPE2_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR1319563.1"
                     /translation="MSKETKSKRFISIRLRLFLQVGAIVLIAVTLILALNKWYLSSIY
                     ILNQKRTMQDLAEEIDTYDVSAADYSSKIALLEKDNGVTIDVYMSDGTPLYYGAVPTG
                     ITGGKIVIKDRHDNPDGSFFEIQENAMSDTQFIVYGKSLKFGGDLEMYSKKTTIDSYA
                     DTAINVMLITSILALCATLVAIYFYSKKFTKPLIEMSSVTGGMADMDFSKKCEYGGND
                     EIGTLASSINELSDSLNSTLVDLNEKNRRLQEDIEKEQQLDKIRKDFISNVSHELKTP
                     ISIIQGYSEG"
     gene            complement(861..>1297)
                     /locus_tag="ACZPE2_06650"
     CDS             complement(861..>1297)
                     /locus_tag="ACZPE2_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1319564.1"
                     /translation="SARSQDFDQLAGFESGADDYVTKPFSPAVLMKRVEALLRRSGSG
                     FVSDTDELWGLSVNKDAHEVKVYGEAVSLTLKEYSILIKLLGFVGRVYSREQLLDDVW
                     GFDYDGDIRTVDSHVARLRTKLGDWGTKHLHTVYGVGYKIEE"
BASE COUNT          305 a          316 c          290 g          386 t
ORIGIN      
        1 accctccgaa tagccctgta ttattgaaat cggagttttc aactcgtgcg aaacattaga
       61 gataaaatcc ttgcggattt tgtcgagctg ctgttctttc tctatatcct cctgcaacct
      121 gcggtttttc tcgtttaaat cgacaagcgt gctgttgagc gaatcagaca gctcgtttat
      181 actgcttgcc aaagtgccta tttcatcgtt tccgccgtat tcgcatttct ttgagaagtc
      241 catatctgcc atgccgccgg tgacgctact catttcaata agcggctttg taaatttctt
      301 tgagtaaaaa tatattgcaa caagcgtggc acaaagagcg agtatgctgg ttataagcat
      361 tacgtttatg gctgtatcgg catatgagtc tatcgtcgtt tttttgctgt acatctcaag
      421 gtctccgccg aatttaagac tttttccgta gactatgaat tgcgtgtcgc tcatagcgtt
      481 ttcctgaatt tcaaagaaag agccgtcggg gttgtcgtgt cggtccttta tcactatttt
      541 acctccggtt ataccggtag gcacggcacc gtagtaaagg ggagtgccgt ccgacatata
      601 tacgtctatg gtaactccgt tgtctttttc gagcaatgcg attttagagc tgtaatcggc
      661 ggcggaaacg tcgtaggtgt cgatttcctc cgcaagatcc tgcatggtac gcttttgatt
      721 gagtatgtat attgacgaga gataccattt gttgagggcg agtatcagcg ttacggcaat
      781 aagaactatg gctccgacct gtaaaaacag ccgcagcctt atacttatga agcgcttact
      841 cttagtctct ttgctcatat tcactcctct atcttatagc ctacgccgta tacggtatgt
      901 aaatgctttg ttccccagtc gccgagcttt gtacgaagcc ttgcaacgtg agaatctacg
      961 gttcttatat cgccgtcgta gtcaaagccc caaacatcgt caagcagctg ttcgcgtgag
     1021 taaactctgc cgacaaagcc gagcagcttt attagaatac tgtactcctt aagcgtcagc
     1081 gaaacggctt cgccgtagac cttgacctcg tgcgcgtctt tgtttaccga caacccccaa
     1141 agctcgtcgg tgtcggagac aaatccgctc ccggagcggc gcaaaagagc ctccactcgt
     1201 ttcataagca cggccgggga aaacggtttt gttacatagt cgtctgcacc gctttcaaat
     1261 ccggctaatt gatcaaagtc ctgacttctt gccgaaa
//
LOCUS       DBMDVO010000474         1297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49099_length_1297_cov_1.145485, whole genome shotgun sequence.
ACCESSION   DBMDVO010000474 DBMDVO010000000
VERSION     DBMDVO010000474.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49099_length_1297_cov_1.145485"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..>1297
                     /locus_tag="ACZPE2_06655"
     CDS             271..>1297
                     /locus_tag="ACZPE2_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319565.1"
                     /translation="MDPINVDFTGRGRDDGKKGVADVLIPPENSGKKIAINIILTLIL
                     GAVLYYFMIPALNFKSIELYLFVVFVCLIYLLLTIISSRAFIKPEYLPYVKRRSRVPG
                     ILILALAAVALVGWLTGVTLFRAKSYSKLISVQDGDFAEDVAEIDFSSVPVFVVSPYS
                     TQINYKNTPVRVTALAYGDIFKWIKNTKEGLPAYIIVDMTTQEGQLVRLPEGMKYSPT
                     EHFNKYLLRYLRFKYPTYLFDEPSFEIDESGSPYWIVPIVDKTIGLFGGTDIDGAIIV
                     NAVTGECHMISTSNDGTTKLPTSSFASDPEWMWIDRIYSPTILTQQYNYYGKLNNGFI
                     NSVIGQEG"
BASE COUNT          355 a          280 c          296 g          366 t
ORIGIN      
        1 atctcccttt cggttttatg aaaaataact tctctgatac aattaaatat tataacctca
       61 aaccggattt ttctcaacta aaaaaattct aaatctttaa aaaattacgg taaaatttgt
      121 gtgtatttcg acacacggta tattttgacg cgttataagc cgaaaaaaag ccttttcgtt
      181 attcgacgga agttcaaaat cgtttaaatc cttgcaaagc gcggtaaaat actatatact
      241 tttttcagaa agtgaaaagg gagtgacatt ttggatccta taaatgttga ttttacggga
      301 agaggcagag acgacggcaa aaagggcgtc gcggatgtgc tcataccgcc cgagaattcg
      361 ggtaaaaaaa tagcgataaa tatcatattg acgcttatac tcggtgccgt gctgtattat
      421 tttatgatac cggcgctcaa ttttaagtcg atagagcttt atctcttcgt cgtttttgtg
      481 tgtcttatat atctgcttct gacgattatt tcgtcaaggg cgtttataaa gcccgaatat
      541 ctgccctacg tcaaaagacg gtcgagggtt ccgggtatct tgattctcgc tcttgccgca
      601 gtggctcttg tgggctggct taccggcgtt acgctgttca gggcaaagtc ttacagcaag
      661 cttataagcg tccaggacgg cgattttgcg gaggacgttg ccgagataga tttctcaagc
      721 gttcccgtgt ttgtcgtatc gccgtattct acgcagataa actataaaaa cacgcccgtc
      781 agagttaccg cgcttgctta cggcgacatc ttcaaatgga taaaaaacac caaggagggt
      841 ctgccggcat atatcatagt tgatatgacc acgcaggagg ggcagcttgt ccgcctgccg
      901 gagggtatga agtattcgcc caccgagcat tttaataagt atcttctcag atatttgagg
      961 tttaaatacc ccacctatct ctttgatgag ccgtcgttcg agatagacga gagcggctct
     1021 ccgtattgga tagttccgat agtcgataag accataggtc ttttcggcgg caccgacata
     1081 gatggtgcga ttatagtaaa cgccgtcacg ggtgaatgcc atatgatatc cacaagcaat
     1141 gacggtacga caaagctgcc gacctcgagt ttcgcttcgg accccgagtg gatgtggata
     1201 gaccgcattt attccccgac gattctcact cagcagtata attactacgg aaagctcaac
     1261 aacggcttta taaactccgt aatcggccag gagggcg
//
LOCUS       DBMDVO010000475         1295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49211_length_1295_cov_1.105528, whole genome shotgun sequence.
ACCESSION   DBMDVO010000475 DBMDVO010000000
VERSION     DBMDVO010000475.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49211_length_1295_cov_1.105528"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="dapA"
                     /locus_tag="ACZPE2_06660"
     CDS             <1..812
                     /gene="dapA"
                     /locus_tag="ACZPE2_06660"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1319566.1"
                     /translation="LKEILEYQIANSTDCIVICGTTGESATLSHEEHTEVIKYTVDVV
                     AGRIPVVAGTGSNDTAYALNLSNEAEKAGVDGLLMVTPYYNKASQEGLIKHFTYVADR
                     VSTPIILYNVPSRTGCEIKPETYAELSKHKNIYAAKEATGNLSSIAKTISLCSDDFAV
                     YSGNDDQITPIMSLGGKGVISVLSNVMPQEAHDIAAAALDGDFKKSAELQLKYLELCN
                     ALFMDVNPIPVKVAMRMMGFDVGPLRLPLCDMSEEKTEHLRKVLAKYGLIK"
     gene            839..>1295
                     /locus_tag="ACZPE2_06665"
     CDS             839..>1295
                     /locus_tag="ACZPE2_06665"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_function: GO:0016726 - oxidoreductase activity, acting
                     on CH or CH2 groups, NAD or NADP as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1319567.1"
                     /translation="MIRIILSGCFGKMGHVIRDCVSSRNDCEIVAGVDIGDGKAEFPV
                     FKSFDSISGVDADVIIDFSHPSVLSGLLEYASAKKCPAVVATTGLSEEQIADINECSK
                     TVPMFFSANMSIGVNLMSELAAKAARVLEGSFDIEIVEAHHNQKIDAPSG"
BASE COUNT          371 a          266 c          294 g          364 t
ORIGIN      
        1 cacttaaaga aattcttgaa taccaaatag ctaattcaac cgattgtatc gttatatgcg
       61 gtaccacagg tgaaagtgcc acactttctc atgaggagca cacagaagta atcaagtaca
      121 cagttgatgt tgttgccgga agaattcccg tagtagcagg aaccggaagc aatgacacag
      181 catatgcttt gaatttgtca aatgaggccg aaaaagccgg cgttgacggt ctgcttatgg
      241 taactcctta ctataacaag gcttctcagg aaggtcttat taaacacttt acttatgtag
      301 ccgatcgtgt ctccacaccg attattttgt acaatgtacc gagtagaacc ggttgtgaaa
      361 ttaaaccgga aacttatgcc gaactttcaa aacataaaaa catttatgcc gctaaagagg
      421 ctacgggaaa tttgtcttca atagcaaaaa ccatttccct ttgcagcgat gattttgccg
      481 tttattcagg taatgatgat caaataactc cgattatgtc tctcggcggc aagggcgtta
      541 tctcggttct ttcaaatgtt atgccgcagg aggctcatga tattgccgct gccgcacttg
      601 acggtgactt taaaaagagt gccgagcttc agcttaagta tctcgagctt tgcaacgctc
      661 tgtttatgga tgttaatccc atccccgtca aggttgcaat gcgtatgatg ggctttgacg
      721 tcggtcctct cagactgccg ttatgcgata tgtccgagga aaagaccgaa cacttgagaa
      781 aagttttggc aaagtacgga cttattaagt aattattaag atttacggat gtgaaaaaat
      841 gataagaata atcttaagcg gttgcttcgg caaaatgggc catgttatac gagactgtgt
      901 ttcctcgaga aacgactgcg aaatagtcgc cggcgtagat ataggtgacg gaaaagcgga
      961 atttccggtg tttaaatctt ttgactcaat cagcggtgtt gatgccgatg tgataattga
     1021 tttctctcac ccgtctgttt tgagcggact tttggaatat gcttctgcaa aaaagtgccc
     1081 tgcggtcgta gctaccacag gcctcagcga agagcaaatt gccgatataa atgagtgctc
     1141 aaaaacagtc cctatgtttt tctcggcgaa tatgtctatc ggcgtaaatt taatgtctga
     1201 gcttgccgcc aaggctgcac gtgtacttga aggctctttt gatattgaaa ttgtcgaagc
     1261 gcaccataat caaaagattg acgcgccctc aggaa
//
LOCUS       DBMDVO010000476         1294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49252_length_1294_cov_1.216262, whole genome shotgun sequence.
ACCESSION   DBMDVO010000476 DBMDVO010000000
VERSION     DBMDVO010000476.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49252_length_1294_cov_1.216262"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="ACZPE2_06670"
     CDS             complement(<1..257)
                     /locus_tag="ACZPE2_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1319568.1"
                     /translation="MSIEEILEAMDIELDKSKNVPLTRGKSLIDVEQFRDLIGQVRLN
                     LPGEIKQAQALVNDRRVIINDAKAEAESIIRKAEEKAKAMVS"
     gene            complement(309..797)
                     /gene="coaD"
                     /locus_tag="ACZPE2_06675"
     CDS             complement(309..797)
                     /gene="coaD"
                     /locus_tag="ACZPE2_06675"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1319569.1"
                     /translation="MKTAIYPGSFDPVTYGHLEIISRASKLFDKVIVLVSVNPLKPCS
                     FTIDERKQFLRESVVAEGIGNVEVDSNEGLLIDYFNEHNADVIVKGLRAISDFEYEFQ
                     MAQANRKLCYKAETIFLTSKSEYTYLSSSMVKQIAMFGGDISDFVPECILDRINERLK
                     RY"
     gene            complement(801..>1294)
                     /gene="rsmD"
                     /locus_tag="ACZPE2_06680"
     CDS             complement(801..>1294)
                     /gene="rsmD"
                     /locus_tag="ACZPE2_06680"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1319570.1"
                     /translation="RPTKDSVKEAIFSAIQFETEGSVVLDLFSGSGQLGIEAVSRGAK
                     KAYLVDSSQNSIKFIKQNVAHVGFESQCEIVNMPNSAFLRTTGEKFDIALLDPPYEKS
                     LIQRSLPALTEKMKDTGVIICEHEPGCRLPEEINGFVITKSKKYGKTALTFYRKPQTE
                     DEE"
BASE COUNT          293 a          304 c          258 g          439 t
ORIGIN      
        1 gataccatag ctttcgcttt ttcttcggct tttctgatta tgctttcggc ttctgcctta
       61 gcatcgttaa taatcactct gcggtcgttt acaagcgcct gtgcctgctt tatttcgccg
      121 ggaaggttca gccttacctg tccgataaga tctctgaact gctcaacgtc aataagggat
      181 ttacctcttg tcagaggaac gttcttcgat ttgtcaagct caatgtccat tgcctcaaga
      241 atttcttcaa tgctcatttt aataccgtcc tttcaaacat ctgatttatt tttacgaaat
      301 ataattcgtc agtatctttt cagcctttcg tttattctgt cgagtatgca ctccggaacg
      361 aaatcgctta tatctccgcc gaacatcgct atctgcttta ccatactgga cgacagatag
      421 gtatactcgc tctttgaagt caggaatatc gtttcggctt tgtagcacag ctttctgttg
      481 gcttgtgcca tctggaattc gtattcgaag tcagaaatag cacgaagccc ctttacgata
      541 acgtctgcgt tatgctcgtt gaaatagtcg atgagaagtc cttcgttgct gtcgacttca
      601 acatttccta tgccttccgc cacaacgctt tcacgcagga actgttttct ttcatctatt
      661 gtaaaactgc atggcttgag cggatttacc gaaacaagca cgattacttt atcaaacagc
      721 tttgacgctc ttgaaataat ctcaagatga ccgtatgtta cagggtcaaa gctaccggga
      781 tatatagctg ttttcatatt ttattcctcg tcttccgtct gtggttttct gtagaatgta
      841 agtgcggttt tgccgtattt ttttgatttt gtaatgacaa atccgtttat ttcttcgggt
      901 aatctgcagc ccggttcatg ctcgcatatt attacgcccg tatccttcat tttctcggta
      961 agagccggca gagaacgctg tataaggctt ttctcatacg gcgggtcgag cagggcaatg
     1021 tcgaatttct ctccggtggt tcttaagaat gcggagttgg gcatattgac aatctcgcac
     1081 tgactttcaa agccgacgtg ggctacattc tgctttatga attttatcga attctgactg
     1141 ctgtccacaa gatacgcttt tttggcgcct ctgcttacag cctctatccc aagctgaccg
     1201 gagcctgaaa ataaatcaag cacaacgctt ccttctgttt caaactgtat agcgctgaat
     1261 attgcttctt ttacgctgtc ctttgtggga cgca
//
LOCUS       DBMDVO010000477         1294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49263_length_1294_cov_1.092205, whole genome shotgun sequence.
ACCESSION   DBMDVO010000477 DBMDVO010000000
VERSION     DBMDVO010000477.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49263_length_1294_cov_1.092205"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ACZPE2_06685"
     CDS             complement(<1..279)
                     /locus_tag="ACZPE2_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012576.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1319571.1"
                     /translation="MQKHTIQDIAQIAGVSPATVSRALNNFPGVRGESRRKILSVAKS
                     LGYAPKIAMNRATGDCGSIIGIVIGGDLRNPFYAEIAYHMEKRLKRFGY"
     gene            479..664
                     /locus_tag="ACZPE2_06690"
     CDS             479..664
                     /locus_tag="ACZPE2_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319572.1"
                     /translation="MNNNMNSAMKTVGVSLAVGTAAAVMSTAAKSSSRKRKMKKAAKK
                     AANTINTIVDNMQDMIK"
     gene            729..>1294
                     /locus_tag="ACZPE2_06695"
     CDS             729..>1294
                     /locus_tag="ACZPE2_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1319573.1"
                     /translation="MKKILCIFSALLIVLGAAFFGCGKKNDKAAAEQSETAAPTTEST
                     TAPDPKPVLSGDAALNDGETYTGRAPLPLVDFNVSDPENTRGLSTEKHGYGYGIAKDG
                     KPNDISVNNQKYFETNGYKALCLDTKSTDKVLYLTFDCGYENGYTAKILDTLKEKQVT
                     AAFFCTLPQVKENPEIIARMINEGHIVG"
BASE COUNT          408 a          299 c          285 g          302 t
ORIGIN      
        1 atacccgaat cgtttcagcc gtttctccat atgataagca atttccgcat agaaaggatt
       61 gcgcagatct ccgccgatca cgatgccgat tatcgaaccg cagtctccgg tggcgcggtt
      121 cattgcgatc ttgggcgcat atccaagcga ttttgcaaca gaaaggattt ttcggcggct
      181 ttcgccgcgc acgcccggga aattgttcag cgcgcgggaa acggtcgccg gcgatacgcc
      241 ggcgatctgg gcaatatcct gaatggtgtg tttttgcatt gaaactcccc ctctgtactg
      301 attatagcgc tttctcacat ttgtttcaat gtgtttttat gaatgcctaa taaaaatgtg
      361 aaaaaatttc aaaaacaatt ttaccatatt tttcaaaaat ataaagaact aaacagtatt
      421 tttaccgtcc gttttgcaca agataataat acaatcttat aaaacggagg aaaaaataat
      481 gaataacaat atgaattcgg caatgaaaac agtaggcgtg agtcttgccg tgggaacggc
      541 ggccgcggta atgtcaaccg ccgctaaatc gtcttcgaga aaacgcaaga tgaaaaaggc
      601 ggcaaaaaag gcggcaaaca ccataaatac cattgtcgat aatatgcagg atatgataaa
      661 atagtcgtat caaagtattc tctgagcaaa gcggttttcg ccgctttgtt acataggagg
      721 aagaaaaaat gaaaaaaatt ctctgtattt tttccgcatt gctgatagta ctcggagcag
      781 cttttttcgg atgcggaaaa aagaatgata aagccgctgc cgagcagagc gaaaccgccg
      841 cccccacgac agaaagcacg accgctcccg acccgaagcc ggttttatca ggcgacgccg
      901 ctttaaatga cggcgaaacc tacacgggta gggctccgct gccgcttgtc gattttaacg
      961 tttcagatcc cgaaaacaca cgcggtcttt cgaccgaaaa gcacggctac ggctacggca
     1021 tagcgaaaga cggcaaaccg aacgatatat ccgtaaacaa tcaaaaatac tttgagacaa
     1081 acggctacaa agcgctttgc ctcgacacca aatcaactga caaggtatta taccttacat
     1141 ttgactgcgg ctacgaaaac ggctacacgg caaaaattct tgacacatta aaggaaaaac
     1201 aggttaccgc cgcatttttc tgcacacttc cgcaggtaaa ggaaaatccc gaaatcatag
     1261 cgcgaatgat aaacgaaggc catattgtcg gaaa
//
LOCUS       DBMDVO010000478         1291 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49414_length_1291_cov_1.050420, whole genome shotgun sequence.
ACCESSION   DBMDVO010000478 DBMDVO010000000
VERSION     DBMDVO010000478.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49414_length_1291_cov_1.050420"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(157..>337)
                     /locus_tag="ACZPE2_06700"
     CDS             complement(157..>337)
                     /locus_tag="ACZPE2_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319574.1"
                     /translation="ADSISKINPDMLSVKLDLTSYDVKEGEQKIPVLITVTGDAAVWA
                     HGTYYVTINSTASEQ"
     assembly_gap    338..347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<348..1265)
                     /locus_tag="ACZPE2_06705"
     CDS             complement(<348..1265)
                     /locus_tag="ACZPE2_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1319575.1"
                     /translation="MDNSVPAQLNLQIFGDNNYTVDVTVKGKKFIVSALTADDFSVVA
                     QTNYVDSAGKKSLQLKATSGKSDNFDIVGLSKNYIEVYFDTYKETEMALKPSIIAPGN
                     KTVTDGCILGNIVFSKNTVIVSGPSTEVNKVTSVTAETSISAPLTATTTVTPELKLVG
                     AAADELSNVSILEGDTAITMTMPVLKLVELPTTVTFRNAPANYLDGNMIFSVSPSRIS
                     AAVPVEKVDQITSLSVGTIDFSELDAGLNTLNFKASDITDFMITDQSIKNFKVSVNMS
                     GMTSGMFSVPAANVTITGQKQGYNSVVTSN"
BASE COUNT          338 a          296 c          277 g          370 t
ORIGIN      
        1 ttgtatgcct gcgttttcac ccgaaagcgc gggcggatgc atcattttat caaccgcctt
       61 gagagtaaaa ttgcatccgc taaaagaaaa acagaggcaa agacatagaa gaagcattgc
      121 aaataatttc tttttcatat atccccgccc ccttttttac tgctcggaag cagttgaatt
      181 tattgtgaca taatatgtgc cgtgcgccca aacggcggca tcgcctgtta ccgttatgag
      241 aaccggtatc ttttgctcgc cctcttttac gtcataagac gtaaggtcga gtttgacaga
      301 cagcatatcg ggatttattt ttgaaatact gtcggcannn nnnnnnngtt tgaggtgaca
      361 accgaattat agccttgctt ttgaccggtt atcgtgacat ttgcggccgg taccgaaaac
      421 attccggagg tcataccgga catattcacg ctgaccttaa agttctttat cgactggtcg
      481 gttatcataa aatccgttat atctgaagcc ttgaaattaa gcgtattaag ccctgcgtca
      541 agctcagaaa agtcaatcgt gccaacgctc agagacgtta tctggtcaac cttctcgaca
      601 ggtaccgccg ccgatattct cgacggactt accgagaaaa tcatattccc gtcaaggtaa
      661 tttgccggag cgtttctgaa tgtcacggtc gtaggcaatt ctacaagctt taaaacgggc
      721 attgtcattg taattgcggt atcgccctca agtatactta catttgacaa ctcatccgcc
      781 gcggctccga caagtttcaa ttccggagtt acggtagtag tagccgtaag aggtgccgaa
      841 atcgacgttt cggcggtaac gcttgtcacc ttgttaacct cggtagacgg gccgctgaca
      901 atgaccgtat tttttgaaaa tacgatgttt ccgagaatac aaccgtccgt tacagtttta
      961 ttgcccggtg caattatgga cggctttaac gccatctcgg tttctttata tgtgtcaaaa
     1021 tatacttcga tatagttttt ggaaagaccc actatatcaa aattatccga tttgccggac
     1081 gtagctttaa gctgcaaact ctttttgccc gccgaatcaa cgtaattggt ctgcgccaca
     1141 acggaaaaat cgtctgcggt aagtgccgac acaataaatt ttttgccttt gacggtaaca
     1201 tctaccgtat aattattgtc gccgaatatc tgaagattga gctgagccgg cacgctgtta
     1261 tccatatcta ttttaaccgg tacggaggat a
//
LOCUS       DBMDVO010000479         1288 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49576_length_1288_cov_1.230834, whole genome shotgun sequence.
ACCESSION   DBMDVO010000479 DBMDVO010000000
VERSION     DBMDVO010000479.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49576_length_1288_cov_1.230834"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="ACZPE2_06710"
     CDS             complement(<1..1089)
                     /locus_tag="ACZPE2_06710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1319576.1"
                     /translation="MNNNKKSFDKKWYSEEFLNELKRTAYAEADGNLTYTAKYAPDAV
                     SKYGIDPRMLGGPIGKMARKLKLVPDFVLNSIHLKITERSLGFFRKGCDRISYANCHI
                     KGVSIKNKHIKASDGFDIPIRIYENAECEGTRTAMIFIHGGAFVGGTLAPYDESLKML
                     VDKFAVKVIGIDYRLLPENAYPAPYSDCFDVLEYISRSCGEFKIDKDRIFVCGDSAGG
                     NIAQACSTKYKGTGKIKAQLLLYPTLNMFGFTDEYYKKGYSDYKFEPSQKAVSKGVIK
                     QMQMLTHCNFKQIGILSPDEYNNPYIFDASGNVPTFITVGALDYLKKDAVAWAHKLSN
                     ANVKTKLVVYNGLGHGYLNATGVFPQAED"
BASE COUNT          338 a          266 c          260 g          424 t
ORIGIN      
        1 gtcctctgct tgcggaaaaa ctccggttgc gtttaaatat ccgtggccga gtccgttgta
       61 aacgacaagc tttgttttta catttgcgtt tgaaagctta tgagcccaag caacggcgtc
      121 ttttttcaga tagtcaagcg cgcctactgt aataaatgtc ggaacattgc cgcttgcgtc
      181 gaaaatgtac ggattattgt attcgtcggg cgagagtatg cctatctgct taaaattgca
      241 gtgcgtaagc atttgcattt gttttattac gccttttgaa acagcctttt gtgacggctc
      301 aaatttgtag tcggaatatc cttttttata atactcgtcg gtaaaaccga acatattcaa
      361 ggtaggatag agtagaagct gcgctttgat ttttccggta cccttgtatt tggtagagca
      421 cgcttgagca atatttcctc cggcactgtc accgcagacg aaaattctat ccttgtctat
      481 tttgaattcg ccacagctgc ggcttatata ttccaatacg tcaaagcaat cgctgtacgg
      541 tgcgggatag gcgttttcgg gcaaaagcct gtaatctatg cctattacct taacggcgaa
      601 tttatcaacg agcattttta agctctcgtc atacggcgcg agcgttccgc cgacaaatgc
      661 gccgccgtga atgaaaatca ttgccgtcct tgtgccttca cattccgcat tttcatatat
      721 tctgatcgga atatcaaatc cgtcggaagc tttaatgtgc ttgtttttga ttgaaacgcc
      781 ttttatatga cagtttgcat aagaaatcct gtcgcagccc tttctgaaaa aaccgagact
      841 tctctcggtt atttttaaat gaatactgtt tagaacaaaa tccgggacaa gctttagttt
      901 ccttgccatt ttgcctatgg gtccgccgag catgcgcgga tctatgccgt attttgatac
      961 ggcgtcgggc gcatatttgg ctgtgtaagt taaatttccg tcggcttccg cgtaggctgt
     1021 tctctttaat tcgttcaaga actcctcgct gtaccatttt ttatcaaagc tctttttatt
     1081 attattcata atacaccttc tgccgatgat atggtataac tatatcataa taaggacggt
     1141 tgtcaataaa ataagttaag tgtataatta ttaatataaa gattatgcga aaggtttttt
     1201 atgtctcata ttatcccaac tccgttattt gaaaaaacag atgaaacaaa aagattgttc
     1261 ataaaatctt tgtcgcttga cgaaagaa
//
LOCUS       DBMDVO010000480         1287 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49648_length_1287_cov_1.005902, whole genome shotgun sequence.
ACCESSION   DBMDVO010000480 DBMDVO010000000
VERSION     DBMDVO010000480.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49648_length_1287_cov_1.005902"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="ACZPE2_06715"
     CDS             <1..282
                     /locus_tag="ACZPE2_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319577.1"
                     /translation="QEQRSFSATMGTGQIVFGLAAVIIGTTLFRRVSFIKGTTAVIFG
                     SVIYKACIQIAISLGLPANLLKLITAVLFLAILVLGNLQKGSVKKNVGA"
     gene            269..1042
                     /locus_tag="ACZPE2_06720"
     CDS             269..1042
                     /locus_tag="ACZPE2_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319578.1"
                     /translation="MLELKHISKIYNPGLVTEMCLFDDFNFTVNDGEFVSVIGSNGSG
                     KTSMLNIICGSIPVNAGEVLINGKCVNKKKDFERYAYIGRVYQNPSMGNCPNMTILEN
                     MSLADNKGKPFGLSFALNKKRYDFYREQLSSLGLGLEDKLHVKMGSLSGGQRQAVSLV
                     MATLTPIDFLILDEHTAALDPKTAEIIMELTDKVVKQKKLTAVMVTHNLRYAVEYGTR
                     LVMMDRGNIILDKAGEDKKNTSIDDILAIFNKISIECGN"
     gene            1077..>1287
                     /locus_tag="ACZPE2_06725"
     CDS             1077..>1287
                     /locus_tag="ACZPE2_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319579.1"
                     /translation="MIFKIGFSIKASQLKIYKNDRKQRLFSAFGHFFGRSEGTRTPGI
                     LLPNGPMKAISAKNSFFRPFPLGKPC"
BASE COUNT          374 a          274 c          293 g          346 t
ORIGIN      
        1 caggagcagc gctcgttttc cgcgacgatg ggaaccggtc agatagtgtt cggcctcgca
       61 gcggtgatta taggaacaac gcttttcagg cgcgtaagct ttataaaggg caccaccgcc
      121 gtaatattcg gcagcgtaat ttacaaagct tgcatacaaa ttgcaataag cctcggcttg
      181 cctgcgaatc ttttgaagct tataacggct gttttgttcc tggcaatact tgttctcggc
      241 aatcttcaga aaggctcggt gaagaaaaat gttggagctt aagcatattt caaaaatata
      301 taatcccggg ctcgttacgg agatgtgcct tttcgacgat tttaatttta ccgtaaatga
      361 cggggaattc gtctccgtaa taggctcaaa cggcagcgga aaaacctcca tgctgaatat
      421 aatatgcgga agtatacccg taaacgcagg cgaagttctt ataaacggca agtgcgtgaa
      481 taagaagaag gattttgaac gctatgccta tatcggcaga gtgtaccaaa atccctcaat
      541 gggcaattgc ccgaatatga cgatactcga aaatatgtcg cttgccgaca ataaaggaaa
      601 gcccttcggt ttgtcatttg cgctcaataa aaaacgatat gatttttacc gcgaacagct
      661 ttcgtcgctc ggtctcggac ttgaggataa attgcacgtg aaaatgggct cgctgtcggg
      721 cggtcaacgt caggcggttt cacttgttat ggcaacgctg acaccgatag acttccttat
      781 ccttgatgag cacaccgctg cacttgaccc caagaccgcg gaaataatta tggagcttac
      841 cgataaagtg gttaagcaga aaaaacttac ggctgttatg gtaacgcata atcttcgcta
      901 tgccgtggag tacggcacgc gccttgttat gatggacagg ggcaatatta tacttgataa
      961 ggcgggcgag gataaaaaga atacgtcgat agacgatatt ctcgcaatat ttaataagat
     1021 aagcattgaa tgcggaaatt aacatttttt atataaatac gtatatcaaa gcagctttga
     1081 tttttaaaat cggcttctca attaaagctt ctcaattaaa aatatataaa aatgaccgaa
     1141 agcagagatt attttccgct ttcggtcatt tttttggtcg gagtgaaggg actcgaaccc
     1201 ccggcatcct gctcccaaac ggacctatga aggcaatttc cgctaaaaat agcttttttc
     1261 gcccctttcc gctcggtaaa ccgtgct
//
LOCUS       DBMDVO010000481         1286 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49697_length_1286_cov_1.178903, whole genome shotgun sequence.
ACCESSION   DBMDVO010000481 DBMDVO010000000
VERSION     DBMDVO010000481.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49697_length_1286_cov_1.178903"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="ACZPE2_06730"
     CDS             complement(<1..414)
                     /locus_tag="ACZPE2_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HIR1319580.1"
                     /translation="MEEINSKKTEAFLTAIKTLADEECKSIDEETDRLRVERLRALQK
                     EAEDRYKSYTEYETARIKADCNSAVSECEERSRKELTDLRAELCKKVFDAAGEKINEY
                     TKTDSYREKLISSAKEIAEAFDGGDVELFLRKEDLV"
     gene            complement(434..739)
                     /locus_tag="ACZPE2_06735"
     CDS             complement(434..739)
                     /locus_tag="ACZPE2_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1319581.1"
                     /translation="MKFFLISDNTDAMIGMRLAGIEAKRVTSRDEAEKAFKKALSAED
                     IGILLITAGVAELCPETVKKLKQGNRPLLVTIPDGDGNGRNRDSITEYIRDAIGIKI"
     gene            complement(873..>1286)
                     /locus_tag="ACZPE2_06740"
     CDS             complement(873..>1286)
                     /locus_tag="ACZPE2_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1319582.1"
                     /translation="LATGYAIIKKRNQGRSIKRSVAVNLATFAVLTVLIAALSVSVFA
                     ADDNNASAADASAATTATETVDNSKGMGLLAAGLVTGLAGIGGGIAVAAGAPAAIAAT
                     AEDPKSFGKSIIFVALGESIALYGVVISILILQKV"
BASE COUNT          297 a          331 c          286 g          372 t
ORIGIN      
        1 cacgagatcc tctttgcgta aaaacagctc aacatcccca ccgtcaaaag cctcggcaat
       61 ctcttttgcc gatgaaataa gcttttcgcg gtaagaatcg gtttttgtat actcgttaat
      121 tttttcacct gccgcgtcaa acaccttttt gcaaagctct gcgcgaagat cggtaagctc
      181 ttttcttgac ctttcttcac attcggagac ggcgctgtta cagtccgcct ttatacgagc
      241 ggtttcgtat tcggtatatg acttataacg atcctcggcc tctttttgaa gagcgcgcaa
      301 acgctcaaca cgcaagcggt ccgtttcttc gtctatactt ttgcactctt cgtctgcaag
      361 agtctttatg gcagttaaga acgcctctgt ctttttgctg ttgatttctt ccatgatctc
      421 acctcactgt tctttaaatc tttataccga tggcgtcgcg gatatattcg gtaatggaat
      481 cacgatttct gccgtttccg tcgccgtcgg gaattgtgac aagcagcggc ctgttgccct
      541 gctttaattt ttttaccgtt tcggggcaaa gctcggcaac tcctgcggtt ataagcagta
      601 tgccgatgtc ttccgcagac aaagcctttt tgaaagcctt ttcggcttca tcgcgcgaag
      661 tcacccgctt tgcttcaatt ccggcaagac gcataccgat catagcgtcg gtattatcgc
      721 ttatcaagaa gaatttcata acaattaccc tttgtatccg taatcttttt ggggagcccc
      781 caatgtgccc ttcggcaacc aaaacagctc acagtctgtc ttttttcggt cataaggcgg
      841 cctttgaaag cacggtgccg ccgcaaatcg ttttagacct tctggagaat aagaattgaa
      901 ataacaacac cgtaaagtgc tatggactcg ccgagtgcaa cgaatataat tgacttaccg
      961 aatgacttgg ggtcctctgc ggtagcggct attgctgcgg gtgcgcctgc ggcaacggca
     1021 ataccgccgc cgatacctgc gagacctgtt acaaggcctg cagccaaaag tcccataccc
     1081 tttgaattat caacagtctc ggttgctgtg gttgcagcag aggcgtcggc agccgaagca
     1141 ttgttatcgt ctgcagcgaa aacgcttacc gaaagagcgg caataagaac agtaagcaca
     1201 gcgaaggttg cgagatttac cgcaacggag cgtttaatgc ttctgccctg atttcttttt
     1261 ttgattattg cataccctgt tgcaag
//
LOCUS       DBMDVO010000482         1285 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49755_length_1285_cov_1.185811, whole genome shotgun sequence.
ACCESSION   DBMDVO010000482 DBMDVO010000000
VERSION     DBMDVO010000482.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49755_length_1285_cov_1.185811"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1285
                     /locus_tag="ACZPE2_06745"
     CDS             <1..>1285
                     /locus_tag="ACZPE2_06745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR1319583.1"
                     /translation="DKDIVDACCDEVGADNVYVYGLTWHKSMQELAADINTYVQKIKA
                     DKHVDKVSIAGHSMGGAVLASYLGLYGCDDVSNITMLNSAFTGLDMVGCLFKGEIAVG
                     IDELIPFINQSMNSDTLGKVLDTLKLLQLAVPKLEGFLETELPDGSGRTYKDRIYTEC
                     LVSGFGYTPSLWAFVPDEYYNDAKAVMKAYMEKNQQQSGVSASVIAANWATFERKIDE
                     IHSIQANISSILQRAKASGTSVCIFSNYNLYIAPFTPTADYTSDGVIETNRTSGGATC
                     ARLKTTLGDDYVQARDVGHNCLSEDGIIDASTCMLPENTWFIKNYGHSMFDYRKNGCD
                     LYVRAMTAKTQPTVDTWAEYPQFLVYNAGTHYVAPLTAKLGDVDLDGSITPVDSRLAL
                     RYVNGMEELSPTAKYVADANRSGDISTFDAEYILKM"
BASE COUNT          325 a          306 c          341 g          313 t
ORIGIN      
        1 cgataaggat atagtcgatg cgtgctgcga cgaagtcggt gccgacaacg tttacgttta
       61 cggtcttact tggcataaat ctatgcagga gcttgctgcg gacataaaca cgtatgttca
      121 aaaaatcaag gcggataagc acgtcgacaa ggtgtcgatt gccggccaca gtatgggcgg
      181 agcggttttg gcttcgtatt tgggacttta cggctgtgac gacgtctcaa acataaccat
      241 gctaaattcc gcatttacgg ggcttgatat ggtgggctgt ctctttaagg gcgagatagc
      301 cgtcggcata gatgagctta tcccgtttat aaatcagagt atgaattccg acactctcgg
      361 caaggtgctt gatacactca agcttttgca gcttgcggtg ccgaagcttg agggattcct
      421 tgagaccgag cttcccgacg gcagcggcag aacctataag gaccgaattt ataccgagtg
      481 ccttgtttcc ggcttcggct acacgccgag tctttgggcg tttgtacccg atgaatacta
      541 taatgacgca aaagccgtaa tgaaggcgta tatggagaaa aatcagcagc aaagcggtgt
      601 ctcggcttct gtgatagctg ccaactgggc gacctttgag agaaaaatcg acgagataca
      661 tagtattcag gcgaatattt cttcgatttt acagagagcc aaggcaagcg gcacttctgt
      721 ttgcatattc tcaaactaca atctttatat agcgccgttt acgcctaccg ccgattacac
      781 gagcgacgga gttattgaga caaaccgtac aagcggcggt gccacctgcg cccgtcttaa
      841 aacgactctc ggcgacgatt acgttcaggc gagggacgtc gggcacaact gtctttccga
      901 ggacggcatt atcgacgcct caacctgtat gctccccgaa aacacatggt ttataaagaa
      961 ttacggccat tcgatgtttg attaccgcaa aaacggctgt gacctttatg tgcgggcgat
     1021 gacggcgaaa acgcagccga ccgttgacac ttgggcggag tatccgcagt ttctcgttta
     1081 taatgcggga actcactatg tggctccgct tacggcaaag ctcggcgacg tggaccttga
     1141 cggcagcata acgcccgttg actcaaggct tgctttaaga tacgtaaacg gtatggagga
     1201 gctgtctccc acggctaaat atgttgcgga cgcaaaccgc agcggcgata tttcgacctt
     1261 tgacgcggag tacatcttaa agatg
//
LOCUS       DBMDVO010000483         1284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49817_length_1284_cov_0.774303, whole genome shotgun sequence.
ACCESSION   DBMDVO010000483 DBMDVO010000000
VERSION     DBMDVO010000483.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49817_length_1284_cov_0.774303"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(2..1156)
                     /locus_tag="ACZPE2_06750"
     CDS             complement(2..1156)
                     /locus_tag="ACZPE2_06750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1319584.1"
                     /translation="MYNKKNIRLGVSVVCTLCTALIIGGVIRSEKAQQTKQAFFEPEY
                     MQMSEKASIIASMLERNYIEDFDDTELSEKMYAAMTDAMDDPYTCYLTKEQMTAFTDD
                     SSGRMSGIGVVIAEEDGHCVIKEVLEGSPAEKAGIKAGDIITEIDGTDVSGMPITEIS
                     AMTKGVENTNVNISVMRGTKRLDFNVDRKSIELKYVTGKCDGNIGYIKITEFSQVAAE
                     QFKTMLAELEKQNIKGLIIDLRNNPGGIIDVVAEIADTILPEGLITYTVDKYGNRRDI
                     LSAEGELDMPVAVLVNGGSASASELLAGALQDRKKGIIIGTQTFGKGIVQGLYSLSDG
                     SGLKITIQKYYTPNGVCINGIGITPDYVIENDGNEDLQYNKAMELLKTKD"
BASE COUNT          330 a          311 c          212 g          431 t
ORIGIN      
        1 cttaatcctt tgttttcaaa agctccatag ccttattata ctgcaaatct tcatttccgt
       61 cattttctat gacataatcc ggagtaattc ctattccgtt tatgcagact ccgttgggag
      121 tgtaatattt ctgtatagtt atctttaagc ctgaaccgtc gctaagagaa taaagtccct
      181 gtacaattcc ttttccgaag gtctgtgtac ctattataat accctttttt ctgtcctgca
      241 atgcacctgc cagaagctcc gaggcagacg cagagccgcc gtttacaaga acggcaaccg
      301 gcatatccag ctcaccttcg gcagacagaa tatcccttcg gtttccgtat ttatcaaccg
      361 tatatgtaat aagcccttcg ggaagaattg tgtccgcaat ttccgcaaca acatcaatta
      421 ttccgccggg attgtttctc aaatctatta taagtccttt tatattctgt ttttcaagtt
      481 cggcaagcat tgttttgaac tgctctgccg caacctgtga aaattccgtt atttttatat
      541 atccgatatt gccgtcgcat tttccggtaa catatttaag ctcaatgctc tttcggtcaa
      601 cattaaaatc aagccttttt gttcctcgca taacggatat atttacattt gtgttttcaa
      661 cgccctttgt cattgcagat atttcggtta taggcatacc cgatacatcg gttccgtcta
      721 tttccgttat tatatcaccg gcttttatgc cggctttttc cgccggagag ccttcaagca
      781 cctcttttat tacacaatga ccgtcctctt cggcgataac aacgccaatt ccgctcatcc
      841 ttccggagga atcatcggtg aatgccgtca tttgctcctt tgtgagatag catgtatacg
      901 gatcgtccat ggcatctgtc attgccgcat acattttttc cgaaagctct gtatcgtcaa
      961 aatcctctat gtaattcctt tcgagcatag atgcaataat tgatgctttt tcggacatct
     1021 gcatatattc aggctcaaaa aacgcctgtt ttgtctgctg tgccttttcc gagcggataa
     1081 ctccgccgat aataagtgcc gtacacaatg tacatacaac gcttactccg agccgtatat
     1141 tctttttatt gtacacataa tcatctccgc ataagcctta ataatagctt atgccacaac
     1201 catatattat atatgtaagc attgtacaat tcaagaaatt aatttccact gacaaaggat
     1261 tttataacag caccgtcatt ataa
//
LOCUS       DBMDVO010000484         1283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49869_length_1283_cov_0.952623, whole genome shotgun sequence.
ACCESSION   DBMDVO010000484 DBMDVO010000000
VERSION     DBMDVO010000484.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49869_length_1283_cov_0.952623"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="ACZPE2_06755"
     CDS             <1..492
                     /locus_tag="ACZPE2_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1319585.1"
                     /translation="VSLVSVPNFAGQSYTRISTDEVQNQRFKITAKYEYNNKVNVGYI
                     ISQSIEEGQQVPYGTELVLTVSKGTEYIDIPDVSGKDYADAEKTLTDAGFTVKKVEKS
                     NDGTHQAGKVIASTPSAGTSAESGSEVYLQVWGAAPTTTSASTTKSSNGFGIDIPRIS
                     DIF"
     gene            complement(577..>1283)
                     /locus_tag="ACZPE2_06760"
     CDS             complement(577..>1283)
                     /locus_tag="ACZPE2_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012904.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR1319586.1"
                     /translation="EAAGDDMAVIVHVGAASTKEACILAHHAEKIGVSAVSAVPSVYY
                     RLSEASIEKHWDTIMDSTELPFFIYNIPQLTGYDLSFELFEKMAKKEKVIGIKNSSES
                     TCQTERFRKIAGPDFVIFNGPDEQLLAGRLMGATAGIGGTYGTMPELYLELTRLIETG
                     DIENAKKMQTKINDCIYELLSFGSLYGACKAVMTLRYGIDCGIPRLPMLPVSKDDPKI
                     KTLAEKINRLVAEIKK"
BASE COUNT          331 a          295 c          298 g          359 t
ORIGIN      
        1 gtttcgcttg tctccgttcc caattttgcg gggcagagct atacgagaat ttcgacagac
       61 gaggttcaaa atcagcgctt taagattacg gccaaatatg aatataacaa taaggtgaac
      121 gtaggatata ttatttctca gagcatagaa gagggccagc aggttcccta cggcaccgag
      181 cttgtgctta ctgtcagcaa gggcacggag tatattgata taccggatgt ttccggcaag
      241 gattacgccg atgcggaaaa aactcttacg gatgccggat ttactgtcaa aaaggttgaa
      301 aaatcaaatg acggaacaca tcaggcaggc aaagtaatag cctccactcc gagtgccggc
      361 acatcggcag agagcggcag cgaagtgtat cttcaggttt ggggtgcggc tccgacaaca
      421 acctcggcgt caactacaaa atcctcaaac ggattcggta tagatattcc gagaataagc
      481 gatatctttt gattcaaatc tatatgaggc ttaaataaaa ccaaaggact gcaagggata
      541 attccttgca gtcctttttt atgctttaat cttttcttac ttttttattt cggcgacaag
      601 acggttgatt ttctcagcca gagtcttgat tttcgggtcg tccttactta cgggaagcat
      661 cggaagtctc ggaataccgc agtctatgcc gtacctgaga gtcataaccg ctttgcacgc
      721 tccgtacaga gagccgaatg acaaaagctc gtaaatgcag tcgtttattt ttgtctgcat
      781 tttttttgcg ttttctatat caccggtttc tataagcctt gtgagctcaa gataaagctc
      841 cggcatggtg ccgtatgtac cgcctatgcc tgccgttgcg cccataagcc tgccggcaag
      901 cagctgctcg tccggaccgt tgaatataac aaaatcgggt cctgcgattt ttctgaatct
      961 ctccgtctgg caggtgcttt ccgaggagtt ttttatgcct ataacctttt ctttctttgc
     1021 cattttttca aaaagctcaa aagacaaatc atatccggta agctgtggga tattgtatat
     1081 gaagaacgga agctccgtgg agtccattat tgtgtcccaa tgcttttcga ttgaagcctc
     1141 cgaaagtctg taatatacgc tcggaacagc cgagacggca gaaacgccta ttttttcggc
     1201 atggtgtgcc aaaatgcacg cttcctttgt ggacgcggct ccgacgtgta caataacggc
     1261 catatcgtct ccggcggcct ctt
//
LOCUS       DBMDVO010000485         1283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49875_length_1283_cov_0.862944, whole genome shotgun sequence.
ACCESSION   DBMDVO010000485 DBMDVO010000000
VERSION     DBMDVO010000485.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49875_length_1283_cov_0.862944"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..>1283)
                     /gene="spoIVB"
                     /locus_tag="ACZPE2_06765"
     CDS             complement(111..>1283)
                     /gene="spoIVB"
                     /locus_tag="ACZPE2_06765"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1319587.1"
                     /translation="GIKSIPDSIHLVSDEKLKFNEIYSYRISSGDVSVSVNSENAAKG
                     TLLSEYTVDISALKVIPVKSADVIVSERKYVIPSGDVFGIRMFTKGVVVVGSDDVYTE
                     EGISNPSKTAGLNAGDIILTVNGNNVNSTLEIEKAVQENGGNELKLSVKRGKKVLNLK
                     LTPALSKNDNCYKAGIWVRDSMAGVGTITFIDSASKVFGGLGHAVCDVDTGIVMPLAD
                     GDAVKTKITGCYKGSCGSTGELCGVFQDTNIGTLSLNTACGVYGFLNNIVSTNEAVPI
                     ATKQEVKTGSAKIISTVDEKGPQYYDVRIVRICNNDSSSSKNMIIEITDSSLIEKTGG
                     IIQGMSGSPIIQDGMLIGAVTHVFVNDPIRGYAVFADNMLKVSEALNAERLLEKAS"
BASE COUNT          358 a          312 c          218 g          395 t
ORIGIN      
        1 tattatcgcc acaacaacag aaaaaagtgc aataaaggca gctgttattt tttacgcaat
       61 atcatcccgt tattaaagta ccggaacggc aaataccgtt ccggtacctt tcacgatgct
      121 ttttcaagca gcctctcagc atttaaggct tcggatactt ttagcatatt atcggcaaaa
      181 acagcatatc cgcgaatcgg atcattaaca aacacatgcg ttacagcgcc gataagcata
      241 ccgtcttgaa taatgggact gccgctcata ccttgaatta taccgcctgt tttttctatt
      301 aaagagctgt ctgtaatttc aattatcata tttttagatg agctgctgtc gttattacaa
      361 attctcacga ttcttacatc atagtactgc ggaccttttt catctacagt ggatattatt
      421 tttgccgaac cggttttaac ctcttgcttt gttgcaatgg gtacggcctc atttgttgaa
      481 acgatattat tcaaaaaccc gtatactccg caagctgtat tcaacgaaag tgttcctata
      541 ttagtatcct gaaatacgcc gcaaagctca cctgttgaac cgcaagaacc tttatagcaa
      601 ccagttatct ttgttttgac ggcatcgccg tcggcaagcg gcataactat acctgtatca
      661 acatcacata ccgcatgtcc gagtccgccg aacactttgc tggcggagtc aataaaagtt
      721 atcgtaccta cccccgccat actgtccctg acccatatac cggctttata acaattatca
      781 ttttttgaca aagcaggcgt caatttcaga ttcagaactt tttttccgcg ctttaccgac
      841 agcttcaatt cattaccgcc gttttcttgt accgcttttt caatttccaa tgttgagttc
      901 acattgtttc cgtttacagt caatattata tccccggcat taaggcccgc tgttttagag
      961 ggatttgaaa ttccttcttc agtatagaca tcatcagaac cgacaaccac aacgcctttt
     1021 gtaaacatgc gtatgccaaa tacatcacct gagggaatta catattttct ctcagaaaca
     1081 ataacatcgg cggattttac cggaataacc ttcagagcgg aaatatccac agtgtattca
     1141 ctaagcagag tgccctttgc cgcattctcg gaattaacgg aaaccgaaac gtcaccgctt
     1201 gatatccggt atgaatatat ctcgttaaat ttcagctttt cgtcactcac caggtgaata
     1261 ctgtccggaa tgctttttat acc
//
LOCUS       DBMDVO010000486         1281 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49964_length_1281_cov_0.959322, whole genome shotgun sequence.
ACCESSION   DBMDVO010000486 DBMDVO010000000
VERSION     DBMDVO010000486.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49964_length_1281_cov_0.959322"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            307..426
                     /locus_tag="ACZPE2_06770"
                     /pseudo
     CDS             307..426
                     /locus_tag="ACZPE2_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012700.6"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
     assembly_gap    427..436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <437..>771
                     /locus_tag="ACZPE2_06775"
     CDS             <437..>771
                     /locus_tag="ACZPE2_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR1319588.1"
                     /translation="ILENATKKSLIILDEIGRGTSTYDGMSIAKAVLEYVTDKHKIGA
                     KTLFATHYHELTELENTLDGVKNYNIAVKKRGDDITFLRRIVRGGADGSYGIDVAKLA
                     GVPESVVRRA"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <782..982
                     /locus_tag="ACZPE2_06780"
     CDS             <782..982
                     /locus_tag="ACZPE2_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319589.1"
                     /translation="SLEAEGIPKKELRGDNKSESGSDFQLSFEQNNNDEILDELKALD
                     VNTLTPIEAMSKLYELSNKAKQ"
     gene            991..>1281
                     /locus_tag="ACZPE2_06785"
     CDS             991..>1281
                     /locus_tag="ACZPE2_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1319590.1"
                     /translation="MPKINRLPKSIYELIAAGEVVERPASVVKELVENSIDAGAKDIT
                     VEIQNGGIKYIRITDNGCGISREDVPLAFTSHATSKISREEDLFSISTLGFRG"
BASE COUNT          395 a          246 c          296 g          324 t
ORIGIN      
        1 gaatatgaag tatttagctc tgtgcgtaaa aaaatcggtg agcagtatga gcgcactcag
       61 cgcacttcaa aggcagtagc gacccttgat gcgctttgca gtctcgcttt tgttgctgtt
      121 tcaaataatt attctcgccc tacagtcaat gcaggagatc gtatatgcat agttaacgga
      181 agacatcctg tcatagaaaa aatgctcggc ggtgcgccat ttgtaccgaa tgatacgact
      241 cttgattgta cagataatcg aacgctcata ataaccggac cgaatatggc aggtaagtct
      301 acctatatgc gtcaggttgc cataattgtt cttatgtctc aaatgggaag ctttgttcct
      361 gccgatagtg ccgaaatagg gcttgttgac agcattttca cacgtgtagg cgcatccgat
      421 gatttgnnnn nnnnnnatcc ttgagaatgc cacaaagaag agtttgatta tactcgacga
      481 aatcggcaga ggtacatcga catatgatgg aatgagtata gcaaaggctg ttcttgaata
      541 tgttacggac aagcataaaa tcggtgcaaa aactctgttt gcaacgcatt atcacgaact
      601 gacagagctc gaaaatactc ttgatggcgt taaaaattac aatattgccg ttaaaaagcg
      661 cggcgatgac ataacatttt tacgcagaat tgtacgcggc ggtgccgacg gcagctacgg
      721 tatagacgtg gcaaagcttg ccggcgtacc ggaaagcgtt gtaagaagag cnnnnnnnnn
      781 ntcacttgag gcagagggaa ttccgaaaaa agaattacgt ggtgataata agtccgaatc
      841 cggcagtgat tttcagcttt cgttcgagca aaacaataac gatgaaatcc tggacgaact
      901 taaagcgttg gatgtcaata ctcttactcc gattgaagct atgtcaaaat tatacgaact
      961 ttcaaataaa gcaaagcaat aggtgttatt atgccgaaaa ttaaccgatt acccaaaagt
     1021 atatatgaac tgattgccgc cggagaggtt gttgaacgac cggcttcggt tgtcaaagag
     1081 cttgttgaaa attcaatcga cgcaggcgca aaagacatta cggtcgaaat acaaaacggc
     1141 ggaatcaagt acatacgtat aaccgacaat ggctgcggaa tttctcgcga ggatgtaccg
     1201 cttgcattta caagtcatgc gacaagtaaa atatcaaggg aagaggacct tttttcaata
     1261 agtacacttg gctttagagg t
//
LOCUS       DBMDVO010000487         1280 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_49995_length_1280_cov_1.304495, whole genome shotgun sequence.
ACCESSION   DBMDVO010000487 DBMDVO010000000
VERSION     DBMDVO010000487.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49995_length_1280_cov_1.304495"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..>1280)
                     /gene="sufB"
                     /locus_tag="ACZPE2_06790"
     CDS             complement(100..>1280)
                     /gene="sufB"
                     /locus_tag="ACZPE2_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR1319591.1"
                     /translation="IANIATYVRPNTKMQNNWKNVPKDIKDTFERLGIPKAERKSLAG
                     VGAQYDSELVYHNVKDEVAAQGVVYSDMESAMNGEYADMVKKYFMKLVTPRDHKFAAL
                     HGAVWSGGSFVYVPKGVQVSIPLQSYFRLNAKGAGQFEHTLIIVDEGASLHFIEGCSA
                     PKYNVANLHAGCVELFVKKNAKLRYSTIENWSKNMYNLNTKRALVEEGGVIEWVSGSF
                     GSHVGCLYPMSILKGDNSRMEFTGVTFAGKGQNLDTGAKVVHVGKNTSSYMNTRSISK
                     GGGISTFRSSVVVEKSAKGTKSSVSCQSLMLDSQSRSDTIPAMDIRTKDAAVGHEAKI
                     GSISNEAVFYLMSRGMSEEDARALIVSGFADNVSKELPVEYAVEMNNLIRLEMKGSIG
                     "
BASE COUNT          325 a          328 c          267 g          360 t
ORIGIN      
        1 tcccaagaaa gagccgtttc atttacgccg agtcgattcc aagtccgtgt cggaagtctg
       61 tttatcaaag tcatataatt caccctttca aaaagacctt tagcctatac tgcctttcat
      121 ttcaagcctt atgaggttat tcatttcaac ggcatattcg acgggaagct cttttgagac
      181 gttatccgca aagccgctta ctataagggc gcgtgcgtcc tcctcactca taccgcggct
      241 cataaggtaa aatacagctt cgttgctgat gctgccgatt ttggcttcgt gaccgactgc
      301 ggcatcttta gtgcgtatat ccatagccgg tattgtgtcc gagcgcgatt gcgaatcaag
      361 catcagagat tggcacgaaa ccgacgactt agtccctttt gcgctctttt cgacaacaac
      421 gctgcttctg aaggtactta ttccgccgcc cttgcttatt gaacgggtat tcatataaga
      481 gcttgtattt tttcctacgt gaacaacctt tgcacccgta tcaaggtttt gacccttgcc
      541 ggcaaaggtg acgcccgtaa actccatacg ggaattatcg ccctttaaaa tgctcatggg
      601 ataaaggcag ccgacatgcg agccaaacga acccgaaacc cactcgataa cgccgccctc
      661 ttcaacaaga gcacgcttgg tgttaaggtt atacatattt ttagaccagt tttcaatagt
      721 tgaatatctg agcttggcat ttttcttgac aaacaattca acgcaacccg cgtgaaggtt
      781 tgcaacattg tatttcggtg cggaacagcc ctcgatgaaa tgtagacttg cgccctcatc
      841 gacaattata agcgtatgct caaactgccc tgcgcccttt gcgttaagtc tgaaatatga
      901 ctgaagcgga attgaaacct gcacaccctt aggcacgtat acgaacgagc cgcctgacca
      961 aacagcaccg tgaagagcgg caaacttatg gtcgcgcgga gttacaagct tcataaaata
     1021 tttttttacc atatcggcat actcgccgtt catggcactc tccatatcgg aatacacaac
     1081 accctgagcc gcaacctcat ctttcacatt gtgatacaca agctccgagt catactgtgc
     1141 gccgacacct gcgagggatt tacgctctgc ctttggaata ccgagtcttt caaaggtgtc
     1201 ctttatatct ttaggcacgt ttttccaatt gttttgcatt tttgtgttgg ggcgaacata
     1261 tgtcgcgata ttagctatat
//
LOCUS       DBMDVO010000488         1278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50088_length_1278_cov_1.192863, whole genome shotgun sequence.
ACCESSION   DBMDVO010000488 DBMDVO010000000
VERSION     DBMDVO010000488.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50088_length_1278_cov_1.192863"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1278
                     /gene="groL"
                     /locus_tag="ACZPE2_06795"
     CDS             <1..>1278
                     /gene="groL"
                     /locus_tag="ACZPE2_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1319592.1"
                     /translation="EVELEDAFENMGAQLVKEVATKTNDVAGDGTTTATLLAQALVRE
                     GMKNVAAGANPMVVKKGMQMAVDAAVEAIKSEAQPVKSSDDIARIATISAADEYIGKL
                     IAEAMEKVTSDGVITIEESKTSETYSDVVEGMQFDRGYISPYMVTDTDKMEAVIDDAY
                     ILITDKKISNIQEILPLLEQIVKAGQKLVIIAEDVEGEALSTILVNKLRGTFTCVCVK
                     APGFGDRRKEMLQDIATLTGGEVITSDLGLELKDTQINQLGRAKQVKVSKENTIIVDG
                     AGDKEAIKSRVAQIKSQIEVTTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEKK
                     LRIEDALAATKAAVEEGCVAGGGVALLNAIPAVKKLLDTDDADLKTGVNIVVKAIEEP
                     VRQIAKNAGLEGSVIVNEIMSSGKKNYGFNFSKE"
BASE COUNT          361 a          281 c          337 g          299 t
ORIGIN      
        1 gaggttgagc ttgaggacgc ttttgagaat atgggcgctc agctcgttaa agaggttgcc
       61 acaaagacaa acgatgtggc aggcgacggt acaacaaccg ctactctttt ggcacaggct
      121 cttgttcgcg agggcatgaa gaatgtagct gcaggtgcta atccgatggt agttaaaaag
      181 ggtatgcaga tggcagttga cgccgctgtc gaagctatca agagcgaggc tcagcccgtt
      241 aaatcttccg acgatatcgc aagaattgct actatttccg cagccgacga atacatcggt
      301 aagctgattg ccgaggctat ggaaaaggta acctccgacg gcgttatcac catagaggag
      361 tctaagacaa gcgaaactta cagcgatgtt gttgaaggta tgcagtttga ccgcggctat
      421 atttcaccgt atatggtaac cgataccgac aagatggaag ctgttattga cgacgcttac
      481 attcttatta ccgataagaa gatttcaaac attcaggaaa ttcttcctct tcttgagcaa
      541 atcgtaaaag cgggtcagaa gctcgttatt atcgctgagg acgttgaggg cgaagctctt
      601 tccacaatcc tcgtcaataa gctccgcggc acattcacct gcgtatgcgt caaggccccc
      661 ggcttcggcg acagaagaaa ggaaatgctc caggatatag ctactcttac aggcggcgag
      721 gttatcacct ccgaccttgg tcttgagctt aaagatactc agataaatca gctcggccgt
      781 gctaagcagg ttaaggtttc taaggagaac accatcattg ttgacggtgc cggcgataag
      841 gaagccatta agagcagagt tgcacagatc aaatctcaga ttgaagttac aacctcagag
      901 ttcgataagg aaaagcttca ggagagactt gctaagcttg caggcggcgt agccgttatc
      961 cgtgtaggtg ctgcaaccga aacagaaatg aaggaaaaga agctccgcat cgaggatgct
     1021 ttggcagcta caaaggcagc tgttgaagag ggttgcgtag caggcggcgg cgttgctctt
     1081 ctcaatgcta taccggctgt taagaagctc cttgacacag acgatgctga ccttaagaca
     1141 ggcgttaaca tcgtagtcaa ggctattgaa gagcctgttc gtcagattgc taagaacgca
     1201 ggccttgaag gttctgttat cgtaaacgaa atcatgagtt ccggcaagaa gaactacggc
     1261 tttaacttct ctaaggaa
//
LOCUS       DBMDVO010000489         1278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50090_length_1278_cov_1.179269, whole genome shotgun sequence.
ACCESSION   DBMDVO010000489 DBMDVO010000000
VERSION     DBMDVO010000489.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50090_length_1278_cov_1.179269"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="ACZPE2_06800"
     CDS             complement(<1..256)
                     /locus_tag="ACZPE2_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319593.1"
                     /translation="MALKLKIRKAAKILSCLTLIAAILCSGLTVFADEPYKGYNFNFY
                     GEASAPNGYLPVKAYNGTEWGVGYLENPTDMYVDKAGYLYI"
     gene            complement(272..1147)
                     /locus_tag="ACZPE2_06805"
     CDS             complement(272..1147)
                     /locus_tag="ACZPE2_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1319594.1"
                     /translation="MRVRKKRVNRSLGGDIGVILLLTIMGLFMGVPLYYTIISAFKPL
                     NELFIFPPRFYVSQPTLSNFKQVFDLASNLWVPFSRYVFNSIFVSVVVTVGHVILASM
                     AAYPLEKAKFPGSKGLFSLVQLTLLFTGGTMAIPLYVVMAYLGLVNSYLALILPALGS
                     SLGLFLMKQFMRGIPNSMLEAAKIDGANELTIFWKLVMPNVKPAWLTLSIFAFQSIWN
                     NTGNNFIYSEELKMLPTALNQIYAADATTGVGNVARQGSAAAVALILMIPPIIFFVLT
                     QSNVIETMAFSGMKD"
BASE COUNT          322 a          305 c          312 g          339 t
ORIGIN      
        1 atatgtaaag ataacccgct ttgtcaacat acatatcggt cgggttctca aggtaaccca
       61 ctccccactc ggtgccgtta tatgccttca ccggaaggta accgttaggt gccgaagctt
      121 cgccgtagaa gttgaagtta tatcccttat agggttcgtc tgcgaaaaca gtgagacccg
      181 aacaaaggat tgcagctatc agtgtgaggc aggaaagaat tttcgcggcc tttctgattt
      241 ttaattttaa agccatcttt tctccctcct atcagtcttt cattccggag aatgccattg
      301 tctcgatgac attgctctgt gtcagcacga agaaaattat cggcggtatc ataagtatga
      361 gtgcaactgc cgccgcactt ccctgacgcg caacgttgcc gacgcctgtt gtggcgtccg
      421 ctgcgtagat ctggttgagc gctgtcggga gcatcttaag ttcctcggaa taaatgaagt
      481 tgttacctgt gttgttccat atcgactgga aggcgaatat cgaaagtgtg agccatgcgg
      541 gcttaacgtt cggcatgacc agcttccaga agattgttaa ttcgttcgca ccgtcaatct
      601 tcgcggcctc aagcatcgag ttgggaatac ctctcatgaa ctgcttcata aggaagaggc
      661 ccaagctgga tccgagtgcg ggaaggatga gcgcgagata ggagtttaca agtccgagat
      721 aagccattac gacataaagc ggaatggcca tcgttccgcc ggtgaacaaa agtgtgagct
      781 gaaccagcga gaacagtccc tttgagccgg ggaactttgc tttctcaagc ggataggccg
      841 ccatcgaagc caaaataacg tgccccactg ttacaacaac actgacaaat atgctgttga
      901 acacgtatct cgagaaggga acccagaggt ttgacgcaag gtcaaatacc tgcttgaagt
      961 ttgacagtgt cggctgtgat acatagaatc tcggcgggaa aatgaaaagc tcgttaagag
     1021 gcttgaatgc cgaaattatc gtgtagtaaa gcggaacgcc catgaaaaga cccattattg
     1081 tgagcagtaa tataacgccg atgtcgccgc caagcgaacg gttgaccctt ttctttctga
     1141 ctctcattcg cttcggagtg actttttccc ttaattcttc gggaaggtcg ttcatattta
     1201 ccgccttgtt ttccgcagtg attattgttt ctgccacgtg tttcccctcc tctcttaatc
     1261 gtctactctg atgagctt
//
LOCUS       DBMDVO010000490         1278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50103_length_1278_cov_0.944775, whole genome shotgun sequence.
ACCESSION   DBMDVO010000490 DBMDVO010000000
VERSION     DBMDVO010000490.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50103_length_1278_cov_0.944775"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>995)
                     /locus_tag="ACZPE2_06810"
     CDS             complement(<1..>995)
                     /locus_tag="ACZPE2_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIR1319595.1"
                     /translation="SGASSMMSSVLRILTSGILAGALIKTGSAEKIAEAIVNKLGQKR
                     AIIALSISTMIICAVGVFIDIAVITVAPIAIAIGRKAKLSKPGILLAMIGGGKAGNII
                     SPNPNTIAAAEGFGVDMTSLMIQNIVPAICAVVVTILISNVLSKKGIAAFEGVEEDSA
                     VSKDLPNFFTAILGPLAVIILLALRPLFGITVDPLIALPIGGFITILVTGNARHTVSF
                     TEFGISKVIGVSILLIGTGTIAGIIKASALQFDLINILNFMNMPAFLLAPLSGILMAG
                     ATASTTAGSTIAAQTFATPLINAGVPAISAAAMTHAGATVIDSLPHGSFFHATGG"
     assembly_gap    996..1005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1006..1163)
                     /locus_tag="ACZPE2_06815"
                     /pseudo
     CDS             complement(1006..1163)
                     /locus_tag="ACZPE2_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
BASE COUNT          372 a          268 c          287 g          341 t
ORIGIN      
        1 aaccgcctgt tgcgtggaaa aatgaaccgt gaggaagcga gtcgataact gttgcgcctg
       61 catgcgtcat tgctgcggca gatattgccg gtacacctgc gttaataaga ggtgtggcga
      121 atgtctgtgc cgcaatcgtt gaacccgcgg ttgtggaagc ggtagcacct gccataagaa
      181 tacctgaaag aggagcaagc aggaatgcag gcatattcat aaagttaagg atatttataa
      241 gatcgaactg aagtgcggaa gccttaataa tacctgcgat tgtacccgta ccgatcaaaa
      301 gaatggatac gcctataact ttactgatac cgaattccgt aaatgaaacc gtatgtcttg
      361 catttcccgt aacaagtatg gttataaatc cgcctatcgg aagtgcaatt aaaggatcca
      421 ccgtgattcc gaaaagaggt cttaatgcaa gtaatatgat tacagctaaa ggaccgagta
      481 ttgcggtaaa gaagttcgga aggtctttag atacggctga atcttcttca acgccctcaa
      541 atgccgcaat tcccttttta gacagaacat ttgaaatcaa tattgtaact acaaccgcac
      601 aaattgcagg tacaatgttc tgaatcataa gcgatgtcat gtcaacaccg aaaccttcgg
      661 cagccgcaat ggtgttaggg ttaggcgata taatgttgcc ggccttgcct ccgccgatca
      721 tggcaagaag aatacctggt tttgaaagct ttgctttcct gcctatggcg attgctatag
      781 gagcaaccgt aataactgca atatcaataa atacgcctac ggcacatatg atcatggttg
      841 agatcgaaag agcgattatg gctcgctttt gtcccaattt atttactatt gcttcggcta
      901 ttttttcggc agatcccgtt ttaataaggg cgccggcgag aatacctgat gtaagaatac
      961 gaagtactga cgacatcata cttgatgcac cgctgnnnnn nnnnnatcgt gcttactgtc
     1021 tgtacaagat ctccgctgcc gataagtccg ccgataagag cgccggcaat gagactgtat
     1081 gccggatgaa ccttacggat tataaggaaa attgaaattc cgagtccgat aagtgctccg
     1141 gcggtagaaa atgtgtaatc catacattct cctttctatt aagaaaaggt cttaatagtt
     1201 ctaaatacct gttcaacggt tgcaatcata tttttttcgg cattatcgtg atccattgct
     1261 tcctgaagag taacaata
//
LOCUS       DBMDVO010000491         1276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50201_length_1276_cov_1.108936, whole genome shotgun sequence.
ACCESSION   DBMDVO010000491 DBMDVO010000000
VERSION     DBMDVO010000491.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50201_length_1276_cov_1.108936"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ACZPE2_06820"
     CDS             complement(<1..279)
                     /locus_tag="ACZPE2_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1319596.1"
                     /translation="MAEKKTIFQEFSKGLLKENPVLRLVLGTCPTLATTTSLESAIGM
                     GLSAAIVLVCSNIVISALRKVIPSKVRIPCYIVVIAGFVTIVQMLVKAY"
     gene            complement(272..850)
                     /locus_tag="ACZPE2_06825"
     CDS             complement(272..850)
                     /locus_tag="ACZPE2_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HIR1319597.1"
                     /translation="MKKKFTAKDILIPTVSLFVICLVVTALLAVTNMLTAPQIQKLSK
                     ETEDKTKAEVLASADEFSDALTVSADGNDYTYYEGTASGDTIGYVFKTSAKGYGGDID
                     LMVGIDTSGKVTGVSILSISETAGLGMNAKNESFINQYIGKSGTIGVSKNGASDTEIQ
                     ALTGATITSKAVTSAVNTALSLYSQIGGEKVG"
     gene            complement(850..>1276)
                     /locus_tag="ACZPE2_06830"
     CDS             complement(850..>1276)
                     /locus_tag="ACZPE2_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1319598.1"
                     /translation="CLGETCAVALLLGFIYLLIRKVISPVIPLCFVGTVAVIMLIAGK
                     GSLTFTAYQLLSGGLLLGAVFMATDYSTSPISAKGKIIFAVGCGIVTSLIRIFGNLPE
                     GVSFSIILMNILVPHIEHLTAPKPFGTLKEKKEKKEAAA"
BASE COUNT          328 a          316 c          287 g          345 t
ORIGIN      
        1 ataagcctta acaagcattt gcactatcgt tacaaagccg gctataacaa ctatgtagca
       61 gggaattctt acctttgaag gaattacctt gcgaagtgcc gaaattacta tgtttgagca
      121 aaccaaaact atggcagccg aaagccccat acctatggca ctctcaagcg atgtggtggt
      181 ggcgagtgtg ggacacgttc cgagaacaag tcttaaaacc gggttttcct ttaaaagtcc
      241 cttggaaaac tcctgaaata tcgttttctt ttcagccaac cttctcgcct cctatctgtg
      301 aataaagact gagcgccgta ttaaccgccg acgtgaccgc ctttgatgtt atcgtagctc
      361 ccgtaagagc ttgaatttcg gtatctgacg cgccgttttt ggaaacgcct atcgtaccgg
      421 attttcctat gtactgattt ataaagctct cgtttttggc attcataccg agacctgccg
      481 tctcgcttat cgagagtatt gacacgcccg taacctttcc gctcgtatca atgcctacca
      541 taaggtcaat gtcgccgccg tagccctttg ccgatgtttt gaacacataa cctattgtgt
      601 cgccggaggc agttccctca tagtaggtgt agtcattgcc gtcggcactc accgtaagag
      661 cgtccgaaaa ctcgtctgcc gaggctaaga cctctgcctt tgttttatcc tctgtctctt
      721 ttgacagctt ttgtatctgc ggggccgtaa gcatatttgt gaccgccaga agagcggtga
      781 ccacaaggca aatcacaaag agtgaaacgg taggaatcaa aatgtccttt gcggtgaatt
      841 tctttttcat cacgctgccg cctccttttt ctcttttttc tccttgagag ttccgaaggg
      901 ctttggagct gtgagatgct caatatgcgg aacgagaata ttcataagaa ttattgagaa
      961 cgaaacgccc tcgggcaaat ttccgaatat tctaatgaga gacgttacaa ttccgcatcc
     1021 cacggcaaat attatttttc cctttgcgct tatcggcgag gtactgtaat cggtagccat
     1081 aaatacagca ccgagcaaaa ggccgcccga caagagctga tatgccgtaa aggtaagact
     1141 gcccttgccc gctatcagca ttattacggc aactgtgccg acaaaacaga gcggaattac
     1201 gggagatatt accttgcgta ttaagagata gataaatccg agaagcagcg cgacggcgca
     1261 cgtttcgccg aggcag
//
LOCUS       DBMDVO010000492         1276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50210_length_1276_cov_0.890213, whole genome shotgun sequence.
ACCESSION   DBMDVO010000492 DBMDVO010000000
VERSION     DBMDVO010000492.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50210_length_1276_cov_0.890213"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1276
                     /locus_tag="ACZPE2_06835"
     CDS             <1..>1276
                     /locus_tag="ACZPE2_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837867.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1319599.1"
                     /translation="YVEKKNGFDVEAKGVLADLKENLSLKGLKDVRIINRYDVSGVSD
                     EEYQKARNLIFSEPPVDNAYDEEIEISEGKVFAVEFLPGQFDQRAASAEECISILTEK
                     ERPTVRSAKVYVLIGDISDEETEAVKNYVINPVEAREASLEKPTSLVMETVVPDDVAT
                     VDGFTEMDESKMEEFLSKMGFAMDMDDLAFCQKYFKDTEKRNPTITELRMIDTYWSDH
                     CRHTTFSTKINDVKINNGKYADVIKAAYDEYKAAKADLYAPDRDTCLMNMATIIVKQL
                     KKQGLLKEIDESEEINACSIVVDVDVNGKDEPWLIMFKNETHNHPTEIEPFGGAATCL
                     GGAIRDPLSGRSYVYQAMRVTGSGDPRVSLKDTHAGKLMQRKITTGASAGYSSYGNQI
                     GLATGQVQEIYDEGYVAKRMEIGAVIGAAPKKN"
BASE COUNT          415 a          212 c          313 g          336 t
ORIGIN      
        1 tatgttgaaa agaaaaatgg atttgacgtt gaggcaaagg gtgtacttgc cgatcttaaa
       61 gaaaaccttt ctttaaaagg tcttaaagac gtcagaatta ttaacagata tgatgtttcg
      121 ggcgtaagcg atgaggaata tcaaaaggcg agaaatctta ttttctcaga acctcctgtt
      181 gataatgctt atgatgaaga aattgaaatc agtgagggta aagtgtttgc ggttgaattt
      241 ttgccgggac agtttgacca gagagccgcg tcggctgaag aatgtatttc aattcttacg
      301 gaaaaggaac gtccgactgt aagaagtgca aaggtatatg ttcttatcgg cgatatttca
      361 gatgaagaaa cagaggctgt taaaaattac gttataaacc ctgttgaggc aagagaggcg
      421 agccttgaaa agccgacttc acttgttatg gaaacagttg ttcccgatga cgttgcgaca
      481 gttgacggct ttaccgaaat ggacgagagc aaaatggaag aatttctttc aaagatgggc
      541 tttgcaatgg atatggacga ccttgcattc tgtcagaagt attttaagga tacggaaaag
      601 cgtaatccta caattacaga acttcgtatg attgatacat attggtctga tcactgccgt
      661 catactacgt tttccactaa gataaatgat gttaagataa ataacggcaa atatgccgat
      721 gtcattaagg ctgcatatga tgaatataag gctgcaaaag ctgatttgta tgcacctgac
      781 agagatactt gccttatgaa tatggcaaca atcatagtta agcagcttaa aaagcaaggt
      841 cttttaaaag aaattgatga atcggaagaa atcaatgctt gttcaatcgt tgttgatgtt
      901 gacgtaaacg gcaaggacga gccatggcta attatgttta aaaacgaaac tcataaccac
      961 ccgacagaaa tcgagccttt cggcggtgcg gcaacttgtc ttggcggtgc tattcgtgac
     1021 ccgctttcag gaagaagtta tgtatatcag gctatgcgtg taaccggtag cggtgatccg
     1081 agagtatcac taaaggatac acatgccgga aagcttatgc agagaaagat tacaacaggt
     1141 gcgtcggcag gttacagttc atacggtaac caaatcggtc ttgcaacagg acaggttcag
     1201 gaaatatatg atgaaggcta cgttgcaaag agaatggaaa tcggtgcggt tataggtgcg
     1261 gcaccaaaga agaacg
//
LOCUS       DBMDVO010000493         1273 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50361_length_1273_cov_1.137372, whole genome shotgun sequence.
ACCESSION   DBMDVO010000493 DBMDVO010000000
VERSION     DBMDVO010000493.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50361_length_1273_cov_1.137372"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="ACZPE2_06840"
     CDS             complement(<1..799)
                     /locus_tag="ACZPE2_06840"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR1319600.1"
                     /translation="MKVKMHTLGCKVNQYETESITEDFLKNGYEITLSDDDADIFVVN
                     SCTVTSESDRKTRQTVRRLKHNHPNSIVILTGCMPQAFPEMAQGLEEADIVIGNKNNA
                     EILPKLRRYLSSHERMIDIEQHCTGEKFYKTSINAFSERTRAIVKIEDGCDRFCSYCI
                     IPKARGRVRSKPIEDIVKETEALAASGFAEVVLVGINLSAYGKDTGERFYDAVKAAAD
                     VDGIKRVRLGSLEPDHLTDEVLCNLAKIDKLCPQFHISLQSGSNSTLK"
     gene            931..1227
                     /locus_tag="ACZPE2_06845"
     CDS             931..1227
                     /locus_tag="ACZPE2_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1319601.1"
                     /translation="MAKTREYAFVYLFGGIMYGLMEVIWRGHTHWTMTLTGGTALLIL
                     HIINIKMSNCNIFLKCLIGASAITLLEFTVGVIINIILKWNVWDYSNRPLNILG"
BASE COUNT          350 a          260 c          249 g          414 t
ORIGIN      
        1 ctttaagggt agaattgctt ccgctctgaa gagagatatg aaattgagga caaagcttat
       61 ctatttttgc aagattgcag agaacctcat cggtaaggtg gtcaggttcg agtgagccga
      121 gacggactct ctttatgcca tcaacatcgg cggcagcctt aacggcgtca taaaaccttt
      181 ctccagtatc cttaccatac gcagaaagat tgattcccac aagtacaacc tccgcaaagc
      241 cggaagccgc taaagcctct gtctctttaa caatatcttc aatcggcttt gagcggaccc
      301 tgccccttgc cttcggtata atgcaatacg aacaaaacct gtcacagccg tcttctattt
      361 ttactattgc tcttgtacgt tcggaaaaag catttatact cgttttgtaa aacttttctc
      421 cggtacaatg ctgctcaata tcaatcattc tttcatgtga agataagtag cggcggagct
      481 tcggaagtat ctctgcattg tttttattgc cgataactat atcagcttcc tcaagtccct
      541 gagccatttc cggaaacgcc tgcggcatac agccggtaag tataacgatg ctgttcggat
      601 gattatgttt cagtctgcga acagtttggc gcgttttacg gtcgctttct gatgtaaccg
      661 tacaggaatt aaccacaaat atgtcggcat cgtcatcaga taaagttatt tcatatccgt
      721 tttttaaaaa atcctcggtt atcgattcgg tttcatactg attaaccttg cagccgagag
      781 tgtgcatttt gactttcatt ttagctccta aaaatccaat ttaagctagt aacgtactgt
      841 attttattat tgtaacataa tagtaagttt ttttccatac tcacggtata taaaacgcct
      901 aagctcacaa aatattttcg gtgattttta atggcaaaga cacgtgaata tgcatttgtc
      961 tacctgttcg gcggtattat gtacggcctt atggaagtaa tatggcgcgg acacacacac
     1021 tggacaatga ctctgacagg aggtacagcc cttttaatac tgcatataat caatatcaag
     1081 atgagtaact gcaatatttt tttgaaatgc ctgataggtg catctgcaat aacattgctg
     1141 gagtttacgg taggcgttat tataaatatt atattaaaat ggaatgtttg ggattattca
     1201 aataggccat tgaatatttt gggataggta tgccctcttt ttttcttttt ttggtactta
     1261 ctatgttttc cgg
//
LOCUS       DBMDVO010000494         1273 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50366_length_1273_cov_1.056314, whole genome shotgun sequence.
ACCESSION   DBMDVO010000494 DBMDVO010000000
VERSION     DBMDVO010000494.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50366_length_1273_cov_1.056314"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="ACZPE2_06850"
     CDS             <1..367
                     /locus_tag="ACZPE2_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319602.1"
                     /translation="VSLYGEKTAPEFETAGRRRIGKALELIYLNYRSHVSVDDAARVS
                     GYSKSHFCRNFKLVTGMSFHSYLESFRITNACALLRSTDYSVGKISESVGYSDTLSFC
                     RAFKSIMGMTPMEYRKSKT"
     gene            complement(476..625)
                     /locus_tag="ACZPE2_06855"
     CDS             complement(476..625)
                     /locus_tag="ACZPE2_06855"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR1319603.1"
                     /translation="MPYIAIRSFPKDGKTKKKVIQKIEEIFVNEWGCPPEAVTISLEE
                     ATPEK"
     gene            complement(645..1184)
                     /gene="rbr"
                     /locus_tag="ACZPE2_06860"
     CDS             complement(645..1184)
                     /gene="rbr"
                     /locus_tag="ACZPE2_06860"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1319604.1"
                     /translation="MANKYTGTKTEKNLEAAFAGESQARNKYTYFASKAKKEGYEQIA
                     ALFLKTADNEKEHAKMWFRELSGIGDTKENLAAAADGENYEWTDMYEGFAKTAEEEGF
                     FELAAKFRLVGEIEKHHEERYRALLANVETAKVFEKSEVKVWECRNCGHIVVGTKAPD
                     VCPTCAHPQSYFEVHAENY"
BASE COUNT          268 a          365 c          269 g          371 t
ORIGIN      
        1 cgtttcatta tacggcgaaa aaaccgcccc ggagtttgag acagccggca ggcgccgtat
       61 cggaaaggct cttgagctga tctacctaaa ctaccgctcc catgtgtcgg ttgacgatgc
      121 cgcccgcgta tcgggctatt ccaagagcca tttttgcaga aatttcaagc ttgtgaccgg
      181 catgagcttt cacagttatc ttgaaagctt ccgcattacc aacgcatgcg cgcttcttcg
      241 ctcgaccgat tattccgtag gcaaaatttc cgaaagcgtg ggctacagcg acacgctctc
      301 gttctgccgc gcctttaaga gcatcatggg catgacgccg atggaatacc gcaaaagcaa
      361 aacataaaag agggtgtgct tcagagcaca ccctcttttt ttagttgcgg atttttccgt
      421 ccaatatcac aacattgtcc atattcgggc ggatctcatt tccgacgacc tcaccctatt
      481 tttccggagt agcctcttcg agcgagatag tgaccgcctc cggcggacag ccccactcat
      541 ttacaaatat ctcttctatc ttttggatga ctttcttttt cgttttgccg tccttaggaa
      601 acgacctgat cgcaatatac ggcatccttt atctcctttt atcatcaata gttttcggcg
      661 tgcacctcaa aataactctg cggatgggca caggtcgggc aaacatcagg cgccttggtg
      721 ccgacaacga tgtgtccgca gttgcggcat tcccacacct tgacctcgct cttttcaaag
      781 acctttgcgg tctcaacgtt tgccaaaagc gctctgtagc gctcctcgtg gtgcttttcg
      841 atctccccga caaggcggaa ttttgccgcc agctcgaaaa atccctcttc ctccgcggtt
      901 tttgcaaatc cctcgtacat atcggtccat tcatagtttt ccccgtccgc cgcggcagca
      961 aggttttctt tcgtatcgcc aattccggaa agctcgcgga accacatttt agcgtgctcc
     1021 ttttcgttat cggcagtctt taaaaacagt gcggcgatct gctcatatcc ctcttttttt
     1081 gccttgctcg caaaatatgt atatttgttg cgcgcctgcg attcaccggc aaatgccgct
     1141 tcaaggtttt tctccgtctt agttccggta tacttatttg ccatattgat tacccctttc
     1201 gttttcagcc cggcacatct gccggttttt ttacagtata taacttccgt ccgcccttgt
     1261 caagcgaaat tgc
//
LOCUS       DBMDVO010000495         1272 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50427_length_1272_cov_0.871904, whole genome shotgun sequence.
ACCESSION   DBMDVO010000495 DBMDVO010000000
VERSION     DBMDVO010000495.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50427_length_1272_cov_0.871904"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="ACZPE2_06865"
     CDS             <1..772
                     /locus_tag="ACZPE2_06865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR1319605.1"
                     /translation="LTRLRENGKFVDKWMGLFHERDFVNYKTVLSFSKDGKMMWTKPE
                     PYFSAYRDIEKKSQMCEVECVRSDMGRGDELMLITRSNSKRINSLLSFSRDEGKTWSR
                     PVAAPASLNGERHKAEYLPDGRLFITFRSIERDLRKNLKHSENKLRGWYSEGWVALVA
                     DYNDLKNGGEGDYRIKVAHTYLDSQSEPCVAANADTGYCGNVILPDGTAVICSYGIFS
                     VEEKKDGVYSTDKGRKKRRTYIISKRINVNDVEKLLGR"
     gene            complement(912..>1272)
                     /locus_tag="ACZPE2_06870"
     CDS             complement(912..>1272)
                     /locus_tag="ACZPE2_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR1319606.1"
                     /translation="YANARICSILRKMKEEGIEPRDCTEEELMLLNAPEERELIRHLS
                     ALTDEIITAAKDYDPARITRYAVTLATLFHKFYNACRVGVDDEKLRAARLYLCCRVKD
                     VMGIILTEFKITVPESM"
BASE COUNT          342 a          278 c          329 g          323 t
ORIGIN      
        1 attgacaaga cttcgagaaa acggaaaatt cgtcgataaa tggatgggac ttttccatga
       61 gcgcgatttt gtaaattata aaaccgtact gtctttttca aaggacggaa aaatgatgtg
      121 gacaaagccc gaaccgtatt tttccgcgta tagggacatt gagaaaaagt cgcagatgtg
      181 cgaggtcgag tgtgtccgca gcgatatggg caggggcgac gagcttatgc tcataacgcg
      241 cagcaattca aaaagaataa attcgctttt gagcttttcc cgtgacgagg gaaaaacgtg
      301 gtcaagaccc gttgcggctc ccgcgtcatt aaacggcgaa agacacaagg cggagtatct
      361 gccggacggc agactgttta taaccttccg ctcgatagag cgtgatttgc gtaaaaatct
      421 gaaacacagc gaaaataagc tccgcggatg gtattccgag ggctgggttg cgctcgtggc
      481 ggattacaac gacctgaaaa acggcggcga gggagattac cgcatcaagg tcgcgcatac
      541 atatcttgac tcgcagtctg agccttgcgt tgcggcaaat gccgacacgg gctactgcgg
      601 aaacgtcatt ttgcccgacg gtactgcggt tatctgttct tacggtatct tttccgttga
      661 ggaaaaaaag gacggtgttt actctaccga taagggcagg aaaaagcgca gaacgtatat
      721 tatttcaaaa agaataaacg tgaatgatgt cgaaaaatta ctcggcagat aaaaacagcc
      781 gaatgttaaa ccggagcttg caaatcacga attttgattt ccgacggaat tgaaaatacg
      841 tataaaaaac cggaaagcga gagcttatga acgctcagct ttccggcttt attatgcttt
      901 tgtgtttttg attacatact ttcgggaacc gtgattttaa attccgtaag tattatgccc
      961 ataacatctt tgaccctgca acacagataa agtcttgcgg cacgtagctt ctcgtcgtca
     1021 acgcccactc tgcaggcgtt gtagaacttg tggaagagtg tggcaagcgt tacggcatag
     1081 cgtgttattc ttgccgggtc atagtccttg gcggcggtga ttatctcgtc cgtcagagcc
     1141 gagaggtggc gtataagctc gcgctcctcg ggcgcattta agagcataag ctcctcttcg
     1201 gtgcagtcgc gcggttcaat gccctcctct ttcatcttgc ggagtatgct gcatattctc
     1261 gcgtttgcgt ac
//
LOCUS       DBMDVO010000496         1271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50479_length_1271_cov_1.076923, whole genome shotgun sequence.
ACCESSION   DBMDVO010000496 DBMDVO010000000
VERSION     DBMDVO010000496.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50479_length_1271_cov_1.076923"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>860
                     /locus_tag="ACZPE2_06875"
     CDS             <1..>860
                     /locus_tag="ACZPE2_06875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1319607.1"
                     /translation="TLGITLYEVLKTADQYEVLKRTSGKLIQFTAMMDRLMSLSETEG
                     LPELYDSIIEETGYVGYLRSNPEKFDDRMQNINELKSNLVRYEEDNEDATLNDYLEEI
                     ALLTDIDNYNAGEDAVVLMTLHSAKGLEFPVVFMPGLEEGIFPGIQSMYNETEVEEER
                     RLAYVGITRAKEKLYITKAKSRMLYGSTNYFHQSRFISEIPENLLNIRQETGFRAMAG
                     LDRGAKHSPYVASAREHHIDRGFSGSGKASQKVTTGDIDYKVGDTVIHKVFGEGVITK
                     TTSMGNDLLL"
     assembly_gap    861..870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1111..1269)
                     /locus_tag="ACZPE2_06880"
     CDS             complement(1111..1269)
                     /locus_tag="ACZPE2_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1319608.1"
                     /translation="MLPVIGITLPFFSAGGSSNLCVYLGIGIVMSIYRHNCDTGAVNI
                     RVYDSLKK"
BASE COUNT          391 a          239 c          287 g          344 t
ORIGIN      
        1 actctcggta ttacgctata tgaggtttta aaaaccgccg atcagtacga ggtgttaaag
       61 cgtacctccg gaaagctgat tcaatttact gcaatgatgg atagactgat gtctctctcc
      121 gaaacagagg gcttgccgga gctatatgac agtattatag aagaaaccgg ttatgtaggc
      181 tatcttcggt ccaatcccga aaagttcgac gaccgtatgc aaaatataaa cgagcttaaa
      241 tcaaaccttg tcagatacga agaagataat gaagatgcaa ccctcaacga ttatctcgaa
      301 gaaatagctt tacttaccga tattgacaat tataacgcgg gtgaggatgc cgtcgttctt
      361 atgacgcttc attcggcaaa ggggcttgag tttccggtgg tatttatgcc cggacttgaa
      421 gagggaatat tccccgggat acagagtatg tataatgaaa ctgaagttga agaagaacgc
      481 aggcttgcat acgtaggaat aacaagggct aaagaaaagc tttatattac gaaggctaaa
      541 agcagaatgt tgtacggctc tactaactat ttccatcaat ccagatttat ctccgaaatc
      601 cctgagaatt tactgaatat caggcaggag acgggattca gggcaatggc gggactcgac
      661 aggggcgcaa aacactcgcc gtatgttgct tctgcaagag aacaccatat agatcgcggc
      721 ttcagcggtt ccggaaaagc ttctcagaaa gtcacaacgg gagatattga ttataaagtc
      781 ggtgacacgg ttattcacaa ggtgtttggg gaaggcgtta ttacaaaaac aacatctatg
      841 ggaaacgatt tactgctcga nnnnnnnnnn ttagttgcga aaaagaggct tttttgtcga
      901 aaaaatattg accttttctt tttaatggat tataataaac ttgctacctg aaaaacggta
      961 gtccgcattt tgcgcaaaag acagcattta gtttttgagg ggctggactg gttgtttttt
     1021 gcgctcactc aaaagagcac ggacaaaaca tgagtttgtc cgtgctcttt tgctttattt
     1081 agttttattt ggtgctgtat aaacagcaga ctattttttt aatgaatcat atactcttat
     1141 atttacggca cccgtatcac agttgtgtct gtaaatgctc ataactatac ctatacctaa
     1201 atagacacaa aggttggaag aaccgccggc actgaaaaac ggaagcgtta taccgatgac
     1261 gggtagcagc a
//
LOCUS       DBMDVO010000497         1271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50489_length_1271_cov_0.945299, whole genome shotgun sequence.
ACCESSION   DBMDVO010000497 DBMDVO010000000
VERSION     DBMDVO010000497.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50489_length_1271_cov_0.945299"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(225..698)
                     /locus_tag="ACZPE2_06885"
     CDS             complement(225..698)
                     /locus_tag="ACZPE2_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319609.1"
                     /translation="MKRIYEKTDPFAGIGETWNHENFEPDLIIQFNWINEYVGKVQRD
                     GIKPEIIKDVYVDMIKMFRRDHPNAKLMMVSRSDQTEFIKILNSAIEEYGKDNDTSWI
                     GILTYDKSVLVNPGHPHPEGQANIAKAFIPQIEKFMGWTAEKLPFTFCVDRVELR"
     gene            complement(712..984)
                     /locus_tag="ACZPE2_06890"
     CDS             complement(712..984)
                     /locus_tag="ACZPE2_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319610.1"
                     /translation="MKKIFTFFLAAAVVLSSFTAVGAADSAAAYVFIESNGKTAKGNV
                     TFNVSKGKTAESLMLNGEEVKEGDGEVYYSESVVSKGVDGTVKIEK"
     gene            complement(1007..1144)
                     /locus_tag="ACZPE2_06895"
     CDS             complement(1007..1144)
                     /locus_tag="ACZPE2_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319611.1"
                     /translation="MKKIYHPPEAKVVLISADDVIMASGIRLTDVSLGVLDIDAVEIY
                     K"
BASE COUNT          323 a          324 c          224 g          400 t
ORIGIN      
        1 tataaccgga ctatctttgc gtcatccatc agtgttcacc tcactttcaa acttaccctt
       61 tcacctatat acacaacttt tgaactcaaa tctcacatat tttttcaaat atttttctat
      121 cccataatac cacaatgttc tacaatttgg gcatgaaaag atgccgaagt tgccttcggc
      181 tcttttcgtt atccagtacc ggttaagtgc gtgatttagt gaaatcaccg gagttctacc
      241 ctatcaacac aaaaggtaaa agggagtttt tccgccgtcc agcccataaa cttctctatc
      301 tgcggaatga acgcttttgc aatgtttgcc tgcccctccg ggtgcggatg acccggattt
      361 acgagcacgg acttatcata ggtcaaaatt ccgatccacg acgtatcgtt atcctttccg
      421 tattcttcaa tagccgaatt taagatcttt ataaactccg tctggtcgga gcgcgaaacc
      481 atcatgagct ttgcgttcgg gtggtcgcgc ctaaacattt ttatcatatc gacgtagaca
      541 tcttttatta tctcaggctt gataccgtca cgctgaacct tgccgacata ttcgttaatc
      601 cagttaaact ggatgattaa gtcaggctcg aagttttcgt gattccacgt ctcgccgatt
      661 ccggcaaacg ggtctgtctt ttcatatatt cttttcatat tcccctccgt cttacttttc
      721 aatttttact gtgccgtcaa cgccctttga aacgacagat tctgaatagt acacctcacc
      781 gtcgccctct ttcacttcct cgccgttcag catcagcgac tcggcagtct tccctttaga
      841 gacgttaaaa gttacatttc cttttgctgt tttgccgttt gattctataa agacgtatgc
      901 cgcggcggaa tcagcggcac cgactgcggt aaacgacgat aatacgaccg ccgccgccag
      961 aaaaaaagta aatattttct tcatcttttt accctccgga tatatttcac ttatagatct
     1021 cgaccgcgtc aatgtcgagc acgccgagtg acacgtctgt caggcggatt ccgcttgcca
     1081 ttatgacatc gtccgctgaa atcagaacca ccttcgcctc aggcggatga tatattttct
     1141 tcacattaat gccccctttt gatataatca tatcataaaa caaataggat taatgttata
     1201 tatttatcaa gaaatgaaac ttatttaaga aaagctgccg ttatgcggca gcttttcctg
     1261 taattcatac t
//
LOCUS       DBMDVO010000498         1270 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50535_length_1270_cov_1.078700, whole genome shotgun sequence.
ACCESSION   DBMDVO010000498 DBMDVO010000000
VERSION     DBMDVO010000498.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50535_length_1270_cov_1.078700"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="ACZPE2_06900"
     CDS             <1..238
                     /locus_tag="ACZPE2_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319612.1"
                     /translation="CIMFSLRNFLMTGFKDAVGKMADYRVILNANGYFEKGVLTESDL
                     AELQILIDRKNNPPKALDSESDEETKTTEQENTK"
     gene            235..495
                     /locus_tag="ACZPE2_06905"
     CDS             235..495
                     /locus_tag="ACZPE2_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319613.1"
                     /translation="MIQTIENIAAVITSVIAIGSIIFAAYRWYLRQNKQDCEIKRIKE
                     ENALICYALSACLDGLIQLGANHTVPIAKEKLDKHLNINAHS"
     gene            507..728
                     /locus_tag="ACZPE2_06910"
     CDS             507..728
                     /locus_tag="ACZPE2_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HIR1319614.1"
                     /translation="MKMKIKKKTKIWVKAALIRAAKTFAQTAAATIGTAALLSDVSWT
                     AVLSAAFLAAVLSLLTSLGGLPEVGENEK"
     gene            718..>1270
                     /locus_tag="ACZPE2_06915"
     CDS             718..>1270
                     /locus_tag="ACZPE2_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319615.1"
                     /translation="MKNKMKSSDEYLLPKELIPDKCNQYSTNMCMAFASCGVLQVLNF
                     KKTGEHKKFSPTYMFERYRKNGGKRGIFESELKTGLEAYGTCFYENCPDYSQSEDSAA
                     LEYVKEHAAELDAIAKPFRARLSKIEGRNRAELFAAIKTAIKERQEPILGAINTESNT
                     NHAVCFVGWDGDKAAYRTSDGFEV"
BASE COUNT          409 a          261 c          318 g          282 t
ORIGIN      
        1 atgtataatg tttagtttga gaaatttttt aatgacggga tttaaggacg ctgtaggtaa
       61 aatggctgac taccgggtaa tacttaacgc caacggctat tttgaaaagg gagttttaac
      121 cgaatctgat ttagcagagc ttcaaatctt gattgacagg aaaaacaatc cgccgaaagc
      181 tttggattcg gaatcggacg aagagacgaa aacgaccgaa caggagaaca caaaatgata
      241 cagacaatcg aaaacatagc ggcggtgatt acatcggtaa tcgcgatagg ctcaataatc
      301 tttgcggcgt accggtggta tcttcggcag aacaagcagg actgcgagat aaagcgaata
      361 aaagaggaaa acgcccttat ctgttatgct ctttcggcgt gccttgacgg ccttattcag
      421 ctcggagcga accacaccgt gcctatcgcc aaagaaaagc ttgacaaaca tttaaacata
      481 aatgcacaca gctaaggagg tattttatga aaatgaaaat taaaaagaaa acaaaaatct
      541 gggttaaagc ggcgctaatc cgtgcagcaa aaacgtttgc gcaaaccgcc gcggcaacaa
      601 tcggaactgc tgccctttta agcgatgtaa gctggacagc cgttttatcg gcggcatttc
      661 ttgcggcggt actgtcgctt ctgacaagcc tcggcggact tccggaggtc ggcgaaaatg
      721 aaaaataaaa tgaaaagcag cgacgaatac ttgcttccga aggagcttat ccccgacaaa
      781 tgcaatcaat atagcacaaa tatgtgtatg gcgtttgcct cctgcggagt tttgcaagtg
      841 ctgaatttta agaaaacagg agaacacaaa aagttttcgc caacatatat gtttgagaga
      901 taccgaaaaa acggtggcaa gcgtgggatt tttgaaagcg agctaaaaac tgggcttgaa
      961 gcttacggca cttgttttta cgaaaattgc cccgactatt cgcaatcgga ggacagcgcg
     1021 gcgcttgagt atgtaaaaga acacgcggca gaacttgacg cgattgcaaa accgtttcgg
     1081 gcaagacttt ccaaaatcga gggaagaaac cgtgcggagc ttttcgcggc gataaaaacc
     1141 gccataaagg aacggcaaga gccgattttg ggagcgatta acacagagtc aaacacaaac
     1201 cacgccgttt gttttgtcgg ctgggacggt gacaaagctg cctaccgcac aagtgacggg
     1261 tttgaggtaa
//
LOCUS       DBMDVO010000499         1268 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50621_length_1268_cov_1.124250, whole genome shotgun sequence.
ACCESSION   DBMDVO010000499 DBMDVO010000000
VERSION     DBMDVO010000499.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50621_length_1268_cov_1.124250"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="ACZPE2_06920"
     CDS             complement(<1..169)
                     /locus_tag="ACZPE2_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1319616.1"
                     /translation="MERLKLNNTNKGIILITLSAFCFALMGMFVRLSGDLPAIQKSFF
                     RNIVALVFSVAV"
     gene            286..561
                     /locus_tag="ACZPE2_06925"
     CDS             286..561
                     /locus_tag="ACZPE2_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319617.1"
                     /translation="MSKINYNGPDVFKPLSPWAYFGLSILFSLPVVGFIFLIIFSVSD
                     ANINRRNFARSYWCIYVIIAVAAVVAVASGVTLGSLENMMAAVRPIA"
     gene            complement(661..>1268)
                     /locus_tag="ACZPE2_06930"
     CDS             complement(661..>1268)
                     /locus_tag="ACZPE2_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319618.1"
                     /translation="KLTLLLDRMYPVFTDLVDLYGFENYDKLFSEYFAAYVPCYEEIY
                     GNKAFTTDDMFSALEGNLGTYMDEYAYNCEVLYGKGKEYADYYLGENKAVLSDDDEKY
                     NAGYTITADTSLVREYDDDEVKAYTSSLTSGQKAAYEHFGISANDISAVAEVKTVKVL
                     SGVGDKDAIAQKNVEFAEHPEIITLVKIGHQWYVDFTADID"
BASE COUNT          330 a          267 c          272 g          399 t
ORIGIN      
        1 gaacggccac ggagaaaacg agcgcgacaa tatttctgaa aaagctcttt tgtattgccg
       61 gcaagtcacc tgaaaggcgc acaaacattc ccatcagtgc aaagcaaaac gccgagagtg
      121 ttatcaggat aattccctta tttgtattat ttaactttaa tctttccata acttataggg
      181 tatcacttct gaatttttaa tcaagatgta tttattacaa atttcttgct ttttcgatcc
      241 tgttatttta caatgaaatt aatctgaaac agggaggaat aaaaaatgtc aaaaatcaat
      301 tacaacggac ccgatgtctt taaaccgctc agtccctggg cttatttcgg tttgtcaatt
      361 ctgttttcgc tcccggtggt gggatttatt ttccttatca tcttcagcgt gagcgacgcc
      421 aatatcaatc gcaggaactt cgcgcgttcg tattggtgta tctatgttat aatagccgtt
      481 gcggcggttg ttgcggtagc ctccggcgtt acactcggtt ctctcgaaaa tatgatggcg
      541 gcggttcgtc cgatagcctg acgaactgtg ccgtattcgg tacagcaata aaaatcgaca
      601 ataaaagcga ctgcttttgc agtcgctttt tatatgttat tgaattttta aaaacgaagg
      661 tcagtctata tcggcagtaa agtcaacata ccattggtga cctattttaa cgagtgttat
      721 tatttcgggg tgctctgcga attcaacgtt tttctgtgct attgcgtctt tgtcgccgac
      781 gccggagagc acttttaccg ttttgacttc agctaccgcg gagatatcgt ttgcggaaat
      841 tccgaaatgc tcataggcgg ccttttgacc gcttgtaagc gatgatgtgt aagccttgac
      901 ttcgtcatca tcgtattcac gtacgagaga cgtgtcggcg gttatggtat aacccgcgtt
      961 gtatttttca tcatcgtcag agagaacagc cttgttttcc ccgagataat aatcggcata
     1021 ttctttgccc ttgccgtaaa gcacctcaca gttataagcg tattcatcca tatatgtgcc
     1081 gaggttccct tcaagtgcag agaacatatc atcggttgta aatgccttgt tgccgtatat
     1141 ttcctcgtag cagggaacat atgccgcaaa atattccgaa aacagcttgt cataattttc
     1201 aaatccgtag agatcgacaa ggtctgtaaa tacggggtac attctgtcga gtagaagtgt
     1261 gagctttt
//
LOCUS       DBMDVO010000500         1265 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50741_length_1265_cov_1.616838, whole genome shotgun sequence.
ACCESSION   DBMDVO010000500 DBMDVO010000000
VERSION     DBMDVO010000500.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50741_length_1265_cov_1.616838"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="ACZPE2_06935"
     CDS             complement(<1..1225)
                     /locus_tag="ACZPE2_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR1319619.1"
                     /translation="MILKALEMQGFKSFPDKTVLEFNKGITAVVGPNGSGKSNISDAV
                     RWVLGEQSTKTLRGSKMEDVIFSGTDVRKAKGFAEVTLRLDNTDRSLNKDSDEVSVTR
                     RYYRSGDSEYLVNGESARLRDVNELFMDTGLGRDGYSIVGQGKIADMVSPKASERRDM
                     LEEAAGISHFRYRRGDAIKRLAQAEENLVRLRDILSELESRVGPLKAQSEKAQKFIVL
                     AGERKNLEIGIWLNTIDKTGEKMRDQEHKIEIAEASHKEAQDELSKIGEMIDKAADGT
                     RDINIKLEEIRNSASGFEEKLSDIDSQIKVAENSILHNNETIERINRDKAAENETEQN
                     IDAAVSAAIECIQKAEEQIADATRQMDELSKQEETYRLSSSEFSDRAAALSGEISALS
                     VRLADCRVTAETANSS"
BASE COUNT          243 a          361 c          278 g          383 t
ORIGIN      
        1 tggaggaatt tgcggtttcc gccgtaactc ggcaatccgc aagccttacg gaaagtgccg
       61 atatttcacc cgaaagagct gccgccctat cggaaaactc ggagctgctc aggcgataag
      121 tctcctcctg ctttgagagc tcgtccatct gccttgttgc gtcggctatt tgctcctctg
      181 ccttttgtat gcattctatt gcagcggaaa cggcggcgtc aatattttgc tccgtctcgt
      241 tctccgcagc cttgtcacgg tttatacgct ctattgtctc gttgttatgt aaaatcgagt
      301 tttcggcaac ctttatctgc gaatctatat ccgaaagctt ttcctcgaag cccgaagccg
      361 aatttctgat ttcctccaat ttaatgttta tgtctcttgt gccgtccgcc gccttgtcaa
      421 tcatctcgcc gatttttgaa agctcatcct gagcctcctt gtgcgacgcc tcggcaattt
      481 ctattttatg ctcctgatcg cgcatttttt cgccggtctt gtctatcgtg ttgagccaaa
      541 tacctatttc gagatttttt cgctcaccgg caagcactat gaatttctgt gccttttcgc
      601 tctgcgcctt taaaggacct actctgctct caagctccga gagtatgtcg cgaagcctta
      661 cgagattttc ctccgcctgc gcgagtcttt taatcgcgtc accgcgtctg taacgaaaat
      721 gtgaaattcc cgccgcttcc tcaagcatat cgcggcgctc gctcgccttg ggcgacacca
      781 tatcggcgat tttgccctgc ccgacaatgg agtaaccgtc cctgccgagg cccgtatcca
      841 taaacagctc gtttacgtcg cggagacgcg cggattcgcc gttcacgaga tattcgctgt
      901 cgcccgaacg gtaataacgc cttgtgactg acacctcgtc gctgtcttta ttaagcgaac
      961 ggtcggtgtt gtcaaggcgc agcgttacct ccgcaaagcc ctttgccttg cggacgtccg
     1021 tccccgaaaa aataacgtcc tccatttttg agccgcgaag cgtttttgtg gactgctcgc
     1081 cgagaaccca gcgcacggcg tcggagatgt tgctctttcc gcttccgttg ggtcctacta
     1141 cggctgtaat gcccttatta aattcaagta ccgttttgtc ggggaaggat ttaaacccct
     1201 gcatttccag cgcttttaaa atcattcttt aaattccttt aaatatcttc tgttatcttt
     1261 atatc
//
LOCUS       DBMDVO010000501         1265 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50781_length_1265_cov_0.905498, whole genome shotgun sequence.
ACCESSION   DBMDVO010000501 DBMDVO010000000
VERSION     DBMDVO010000501.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50781_length_1265_cov_0.905498"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            47..820
                     /locus_tag="ACZPE2_06940"
     CDS             47..820
                     /locus_tag="ACZPE2_06940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR1319620.1"
                     /translation="MEKLRAKKGFICDMDGVIYHGNKLLDGVKEFVDWLYKNDKHFLF
                     LTNSSENSPSDLRQKLARMGLDVDESHFYTSALATARFIKSQCEHAGAYVIGGPGLLN
                     ALYQEGISMNNVNPDYVIVGECRTYNYESLERAVQLVRNGARLIGTNPDITGPIEGGI
                     APACGSLISPVELASGKNAYFVGKPNPLMMRTGLKMLGVHSAEAAMIGDRMDTDIIAG
                     IETGLDTVLVLSGVTTKNDIETFPYRPSYILNGVGDIPG"
     gene            917..1262
                     /gene="rnpB"
                     /locus_tag="ACZPE2_06945"
     ncRNA           917..1262
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPE2_06945"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          358 a          284 c          338 g          285 t
ORIGIN      
        1 tgcatggcgg acatcagtgt acttaggggc gaatgaggag caaactatgg aaaaactgag
       61 agcaaaaaaa ggctttatct gcgatatgga cggcgtcata tatcacggaa acaagttgct
      121 tgacggagtt aaggagtttg tggactggct ttataaaaat gataagcact ttctcttttt
      181 gacaaactca agcgaaaatt caccgtcgga tctgagacag aagcttgctc gcatggggct
      241 tgatgttgac gaaagtcatt tctacacaag cgcactggca acagcgagat tcattaaaag
      301 ccagtgtgaa catgcaggag catatgttat cggcggacct ggtcttttga acgctctcta
      361 tcaagaaggc atctccatga acaacgtaaa tccggactac gtaattgtcg gcgaatgccg
      421 cacatataat tacgaaagtt tagagcgcgc ggttcagctt gtaagaaacg gggcaagact
      481 gatcggaaca aacccggata ttaccgggcc gatcgaaggc ggaattgctc cggcttgcgg
      541 aagccttatt tctcctgtag agcttgcatc cggcaagaac gcatattttg tcggcaaacc
      601 gaacccgctg atgatgagaa ccgggcttaa gatgctcggc gttcactcgg cagaagcggc
      661 gatgataggc gacagaatgg atactgacat tatagccgga attgagaccg gcctcgacac
      721 cgtacttgta ctctcgggcg ttacaacaaa aaacgatatt gaaaccttcc cctaccgtcc
      781 gagctacatc ttaaacggtg taggcgatat tccaggctaa aacatatgcg gcaaaagctg
      841 tgggcatttc gcccatagct tttgttattt aaatttccct ttacaaaagg gggcattgtg
      901 tgctataatg tttaaatcag tatgacagac gatcgcgggg cacatttttg tgcaatgagg
      961 aaagtccggg ctccggggca aaggatgccg ggtaactccc ggtgaaggcg actttaagga
     1021 aagtgcaaca gaaaatatac cgcctctttt tgaggtaagg gcgaaatggc gaggtaagag
     1081 ctcaccggct tcggggtaac ccgaaggcct tgtaaacccc atccggagca acgccggcat
     1141 ggcatcgcag gctaacagac ggcccgtctc tgcgatggcc ggcggcttta gccgcagaga
     1201 gatctgcggc atagatagat gaccgtctga tacagaaccc ggcttacagt catgctgata
     1261 aaaag
//
LOCUS       DBMDVO010000502         1263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_50883_length_1263_cov_0.888124, whole genome shotgun sequence.
ACCESSION   DBMDVO010000502 DBMDVO010000000
VERSION     DBMDVO010000502.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50883_length_1263_cov_0.888124"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="ACZPE2_06950"
     CDS             <1..550
                     /locus_tag="ACZPE2_06950"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1319621.1"
                     /translation="GIGKVNAAMCTQILIDLFNVDAVINTGVAGAVAEGITVGDVVIS
                     SDALQHDMDCSPLGDPIGVIPRMPESIFKADERLVKLAFDASSKVISGKTLKGRVVSG
                     DQFIASKEVKDKLVKNFNGSCAEMEGAAIAHICYMNSVPYVIIRNISDSADGSADVSF
                     VEFCKEAVIHSGKIVLEMMKNI"
     assembly_gap    703..712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <713..>1263
                     /locus_tag="ACZPE2_06955"
     CDS             <713..>1263
                     /locus_tag="ACZPE2_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016202.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR1319622.1"
                     /translation="PTEEERLAEQKRLEEEAAAAEAERQRLEEEAKKNPENLDYKYTI
                     STYSTKYSQNAGNANRNFNMELACKAIDGTILAPGQEFSYNDTLLSKRNPKNDYKPAG
                     VFSGGRVTTGIGGGICQVSTTLCDAALYSKMTITQRRCHSMRVGYVPAGMDATVSWGS
                     VDFRFRNDLTQPVKIQASASNGV"
BASE COUNT          399 a          265 c          282 g          307 t
ORIGIN      
        1 cggaataggc aaagtaaatg ccgcaatgtg tacacagatt ttaattgatt tattcaatgt
       61 tgatgcagta atcaacacag gcgttgccgg tgcggttgcc gaaggaatca ccgtaggcga
      121 tgttgttatt tcatcggatg cacttcagca cgatatggac tgctcaccat taggcgaccc
      181 gatcggcgtt attccaagaa tgccggaaag cattttcaag gctgatgaaa gacttgttaa
      241 gcttgctttt gacgcttcat caaaggttat cagcggcaaa accttaaaag gacgagttgt
      301 aagtggagat cagtttatcg ccagcaaaga ggttaaggat aaacttgtta aaaatttcaa
      361 cggaagctgt gctgaaatgg aaggtgccgc aatagcacat atatgctata tgaacagcgt
      421 tccttatgtt ataatcagaa acatttccga ttctgccgac ggcagtgcag acgtatcatt
      481 tgttgaattc tgcaaagagg ctgttattca ttcaggaaaa atagttcttg aaatgatgaa
      541 aaatatctaa acctatacta ttacaaatac agtattacag ggggaacatt attatgaacg
      601 gattaattgg ggctcttatc attacaaccg cactcagcgg tacaacacct gcagcacttc
      661 cggaggatac ttcgaattac ataccgcata ttgaatccaa cgnnnnnnnn nnccctacgg
      721 aagaagaacg tcttgcagaa cagaaacgcc ttgaagaaga ggctgctgcc gcagaggcag
      781 aaagacagcg tcttgaagaa gaggcaaaga aaaaccctga aaatctcgac tataaatata
      841 ccatttccac atattcgacc aaatatagcc agaatgccgg aaatgccaac agaaacttca
      901 atatggagct tgcctgcaag gcaatcgatg gaacgatact tgctcccggt caggaatttt
      961 catacaacga cacactactc agcaagcgta atcccaaaaa cgattataaa cctgccggag
     1021 tattcagtgg cggtcgtgta acaacaggta tcggcggcgg tatatgtcag gtatcaacaa
     1081 cactttgtga tgcggctctg tattcaaaaa tgacaataac acagagaaga tgccattcaa
     1141 tgcgtgtcgg ctatgttcct gccggaatgg atgccacagt ttcatggggc tcggttgact
     1201 tccgattcag aaacgacctt actcagccgg taaaaataca ggcatcggcc tctaacggcg
     1261 tta
//
LOCUS       DBMDVO010000503         1258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51132_length_1258_cov_1.554019, whole genome shotgun sequence.
ACCESSION   DBMDVO010000503 DBMDVO010000000
VERSION     DBMDVO010000503.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51132_length_1258_cov_1.554019"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="ACZPE2_06960"
     CDS             complement(<1..929)
                     /locus_tag="ACZPE2_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1319623.1"
                     /translation="MAIYENIKLDKGLYGTSKGFLAELESIDPSENYKGTSLEGLDAF
                     ERQLKRFDIKVSGNMSSPVDKFFATTDSAALFPEYISRAVRTGLESADVLSEIIAAKT
                     VIDGLDYRPITSVLTDDDKALKETAEGTAIPETSVKTQENLVKLKKRGRMLCASYEAI
                     RFQKLDLFTVTLKQIGAYISRALFKDAVSVLSLGDGNKNPAATLKVSTAGKLTYSDLV
                     SFWNGFDPYELNTVIASPDIAAAILNMPEMRDAAAGLNFHATGKAVTPLGANMLKSSA
                     AQAGTLIGLDKNCALEMVEAGGILTEYDKLIDR"
     gene            complement(948..>1258)
                     /locus_tag="ACZPE2_06965"
     CDS             complement(948..>1258)
                     /locus_tag="ACZPE2_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319624.1"
                     /translation="FTADGDANRQLTFLKSENERLKGLAKAGQLYRTEKQTELVKAFV
                     AAFPSLGDKLCKKMAECLETDELCAAAKAVSKNNFTLSSPQLTPAKKSETNSNSQFRL
                     "
BASE COUNT          252 a          306 c          339 g          361 t
ORIGIN      
        1 tggcggtcga taagcttgtc gtattcggtg agaataccgc ctgcttcaac catttcgagg
       61 gcgcagtttt tatcaagacc tataagcgtg cctgcctgcg ctgcgctgct tttcagcata
      121 ttagcgccga gcggcgttac ggctttaccg gtcgcgtgga aattaagccc cgccgcagcg
      181 tcgcgcattt ccggcatatt gagtatggct gctgcgatgt cgggtgaggc tataacggtg
      241 ttgagctcat acgggtcaaa gccgttccag aacgaaacga ggtcggaata tgtgagcttg
      301 cccgcagttg aaaccttgag ggtggccgca gggttcttgt ttccgtcgcc gagcgataaa
      361 acgcttaccg cgtccttaaa gagcgcacgg gaaatatatg cgcctatctg ctttaatgtg
      421 acggtgaaca ggtcaagctt ctggaagcgt atagcctcgt atgacgcgca taacattctg
      481 ccgcgctttt tgagctttac gaggttctcc tgagttttaa ccgaggtttc gggtatggcg
      541 gtaccctcgg ctgtctcctt cagagccttg tcgtcgtctg tgagaaccga ggttatcggg
      601 cggtagtcaa ggccgtcgat aacggtcttt gccgcaatga tttccgagag aacgtctgcg
      661 ctttcaagtc ccgttctcac ggctcttgaa atatattcgg ggaagagtgc ggcactgtcg
      721 gtggttgcga agaatttatc gacggggctt gacatattgc cgctgacctt tatgtcaaag
      781 cgcttgagct gacgctcaaa tgcgtcaagt ccctcaagag aggtgccctt gtagttttct
      841 gaggggtcaa tgctctcaag ctccgcgagg aagccctttg atgttccgta aagtcccttg
      901 tcgagcttga tgttttcgta aattgccatt tgttttatcc tttcttttta cagtctgaac
      961 tgcgaatttg aatttgtttc ggatttttta gccggtgtga gctgcggaga gctcaaggta
     1021 aaattatttt tactcacggc tttcgctgcg gcgcaaagct catctgtttc aagacactcc
     1081 gccatttttt tacacagctt gtcaccgagt gacggaaacg cggcgacaaa cgcttttaca
     1141 agctccgtct gcttttcggt gcggtacagc tgtccggctt ttgcaagacc tttcagacgt
     1201 tcgttttcac tttttaagaa cgtcagctgc cggtttgcgt cgccatctgc ggtgaaac
//
LOCUS       DBMDVO010000504         1258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51172_length_1258_cov_0.626621, whole genome shotgun sequence.
ACCESSION   DBMDVO010000504 DBMDVO010000000
VERSION     DBMDVO010000504.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51172_length_1258_cov_0.626621"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            82..>1258
                     /locus_tag="ACZPE2_06970"
     CDS             82..>1258
                     /locus_tag="ACZPE2_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1319625.1"
                     /translation="MSNKNYKFETLQLHVGQEEPDIASGARAVPIYQTTSYVFKNSDE
                     AEARFALKDAGNIYGRLTNSTQDVFEKRIAALEGGVAALATASGAAAIDYTIRSLAVA
                     GDHVVASNTIYGGTYNLLEHTLPDYGITTTFVDPDDISNFENAIQDNTKLLYIETLGN
                     PNSNVADIEEIAKVVHKHKIPLVVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGTA
                     IGGVIVDSGKFDWIGSGKFPSLVEPNPSYHGVSFAKDVGAAAFVTKIRAILLRDTGAT
                     ISPFHAFLFLQGLETLSLRVERHTENALKVVEYLNNHPQVEKVNHPSLASGEQKRLYD
                     KYFPNGGGSIFTFEIKGDDTTAKKFIDHLEIFSLLANVADVKSLVIHPASTTHSQLSD
                     "
BASE COUNT          350 a          302 c          283 g          323 t
ORIGIN      
        1 ggagaaacag ccgaatgtgt acctgttgac ctttaatttt aacaaaacat catttaaaaa
       61 ttattgaaag aggtacacaa aatgagtaac aaaaattata aattcgaaac cttgcagctc
      121 cacgtaggcc aggaagagcc ggatatagca tcgggcgcaa gagccgttcc gatatatcag
      181 acaacatcat atgtttttaa aaactccgac gaagcagagg ctcgtttcgc tcttaaggat
      241 gccggcaata tttacggcag acttacaaac tccactcagg acgtatttga aaagagaata
      301 gctgctcttg agggcggcgt tgctgcgctt gccacggcat cgggtgctgc ggctattgat
      361 tacaccatcc gctctcttgc agtcgccggt gatcacgttg tcgcttcaaa caccatctac
      421 ggcggcacgt ataatcttct tgagcatacg cttcccgatt acggcattac aactaccttt
      481 gtagaccccg acgatataag caattttgaa aacgccattc aggataatac aaagcttctg
      541 tatatagaga ctctcggtaa cccaaattca aatgttgctg acatagagga gatagcaaag
      601 gtagtgcata agcataagat tccgcttgtt gttgacaata cctttgcaac accttatctt
      661 gtaagaccca tagagtacgg tgcggatata gttgttcatt cggctacaaa gtttatcggc
      721 ggccacggta cggcgatagg cggagtgatt gttgacagcg gtaagtttga ctggatcggc
      781 agcggcaaat tcccgtcact tgttgaaccg aacccgagct accacggcgt aagcttcgca
      841 aaggatgtcg gagctgcggc ttttgtcacc aagattcgcg caatacttct ccgcgatacc
      901 ggagctacaa tttctccgtt ccacgccttc cttttccttc agggacttga gacgctctct
      961 cttcgcgttg agcgccatac ggaaaatgca ttaaaggtag ttgaatacct caacaatcat
     1021 ccgcaggttg aaaaggtaaa tcatccgtct cttgcaagcg gcgagcagaa gcgtctttac
     1081 gataaatatt tcccgaacgg cggcggctcg atatttacct ttgaaataaa gggcgatgat
     1141 acaaccgcaa agaagtttat agaccatctt gagatattct ctcttcttgc gaatgttgcc
     1201 gatgtgaaat cgcttgttat tcacccggcc tcaaccactc attcgcagct cagcgacg
//
LOCUS       DBMDVO010000505         1256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51282_length_1256_cov_0.802597, whole genome shotgun sequence.
ACCESSION   DBMDVO010000505 DBMDVO010000000
VERSION     DBMDVO010000505.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51282_length_1256_cov_0.802597"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="folP"
                     /locus_tag="ACZPE2_06975"
     CDS             complement(<1..710)
                     /gene="folP"
                     /locus_tag="ACZPE2_06975"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR1319626.1"
                     /translation="MFFFRGKNFSFELNTRTYVMGILNVTPDSFYDGGKWNSPGAALS
                     HALQMQNDGADIIDIGAQSTRPGHKELSEKEELSIIKEYIDAIIPKLSVPTSVDTFYP
                     AVAEYCLMHGVAVVNDVSGRFNPDIASLVKNYNAGWIVMHTGGGDADRMTDYEENVVG
                     NVNAFFDYMLEKSENFGIKREQICLDIGIGFGKDYEDNLTLIKNIKLLKRQDVMLLTA
                     LSNKRVIKAASHSDAENC"
     gene            complement(712..>1256)
                     /gene="folE"
                     /locus_tag="ACZPE2_06980"
     CDS             complement(712..>1256)
                     /gene="folE"
                     /locus_tag="ACZPE2_06980"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR1319627.1"
                     /translation="KAVREILIAIGEDPDREGLVETPKRVANMYEEIFAGLDSDPHRL
                     LKVFDEGDNDEMVVVRDIPMYSMCEHHLLPFIGKAHIAYIPADGKVIGLSKLARIVSD
                     FAKRPQLQERLTSQIADFLEENLSPKGVAVVVEAEHLCMTMRGARAAGATTFTSALRG
                     SMRKDARTRAEVMSLLRGAK"
BASE COUNT          335 a          314 c          227 g          380 t
ORIGIN      
        1 aaacagtttt ccgcgtcgga gtgtgatgct gctttaatta cacgtttatt tgaaagagcg
       61 gtaagtaaca taacatcctg cctcttcaac agttttatat tttttataag agtaagatta
      121 tcctcataat ctttaccgaa tccgatgcct atatcaagac atatctgttc tctttttatg
      181 ccgaagtttt cactcttttc caacatatag tcaaaaaaag cattgacatt tccgacaaca
      241 ttttcttcgt aatcggtcat cctgtcggca tcaccgccgc ctgtatgcat gactatccaa
      301 ccggcattgt aatttttcac taaggaagca atatccggat tgaatcttcc gcttacgtca
      361 tttacaacag ccactccgtg cattaagcag tattcagcta ccgccggata aaaagtgtca
      421 acagaagtcg gtaccgagag cttcggaatt atcgcatcaa tatactcctt aataattgaa
      481 agttcctctt tctccgaaag ctctttgtgc cccggtcttg tggattgcgc acctatatct
      541 attatatcgg ctccgtcgtt ttgcatttgt aaagcgtggc taagtgctgc accgggcgaa
      601 ttccatttgc cgccgtcata aaacgaatcc ggagttacat ttaaaatgcc cattacatat
      661 gtacgtgtat ttaactcaaa gctgaagttt tttcctcgaa aaaagaacac tttattttgc
      721 tcctctcaaa agcgacatta cttctgcgcg cgttctggca tcttttcgca ttgaaccacg
      781 cagcgccgac gtaaaggtag tggctcctgc cgctctcgct ccgcgcattg tcatacaaag
      841 gtgctctgcc tcaacaacca ccgcaacgcc cttcggagaa agattttcct cgagaaagtc
      901 ggcaatctgc gaagtaagac gttcttgcag ctgtggcctt ttagcaaaat ccgaaacaat
      961 ccttgcaagc tttgaaagtc ctatcacctt tccgtctgcc ggaatatatg cgatatgagc
     1021 tttgccgata aaaggaagta aatggtgctc acacatagaa tacatgggta tatccctaac
     1081 tacaaccatt tcatcattat cgccctcatc aaacaccttt agcagcctat gaggatcgct
     1141 gtcaagtcct gcaaatatct cttcatacat attagctaca cgtttaggtg tttcaacaag
     1201 gccttccctg tcggggtcct cacctattgc tatcagaatt tctctgacag cttttt
//
LOCUS       DBMDVO010000506         1253 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51457_length_1253_cov_0.729167, whole genome shotgun sequence.
ACCESSION   DBMDVO010000506 DBMDVO010000000
VERSION     DBMDVO010000506.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51457_length_1253_cov_0.729167"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="ACZPE2_06985"
     CDS             complement(<1..344)
                     /locus_tag="ACZPE2_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1319628.1"
                     /translation="MTNYIDNGIIKVGVKSFAATLTSIVSKKSGYEFLWQGNPTVWNG
                     QSPILFPIIGKLLNDECIINGKTYSIIRHGLARHREFEPVVLTENEIVFLQKENADTL
                     KCYPYKYELYIT"
     assembly_gap    350..359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(473..>1253)
                     /locus_tag="ACZPE2_06990"
     CDS             complement(473..>1253)
                     /locus_tag="ACZPE2_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1319629.1"
                     /translation="MMKLIPFIIGILAGYVAAAIFTAIGIKTNNTALQIIDFSVFSNM
                     KLFAVPDFTFIEALKGTKEITGQYVATIAVAYVPVAFVVFAEHIADHKNLSSIINKDL
                     LEEPGLHRTLLGDGVGSMVGAVFGGCPNTTYGESVGCVAITGNASVVTILATAIMAII
                     ISFFGPFVTFLASIPNCVMGGVCITLYGFIAVSGLKMIQKVNLDENRNLFVVAVILIA
                     GIGGLTINFGSVTLTSIACALILGIITNSILPKDKEISAAE"
BASE COUNT          373 a          245 c          271 g          354 t
ORIGIN      
        1 aaagtgatat aaagctcata tttgtaagga tagcatttca gcgtatctgc attttccttt
       61 tgtaagaaca caatctcgtt ttctgtcaat actaccggtt caaactctct gtgccttgca
      121 agaccgtgac ggattatgct gtatgttttc ccgttgataa tacattcatc atttaacagc
      181 ttaccgatta ttggaaacag aataggggac tgaccgttcc acaccgtcgg attgccttgc
      241 cataaaaact catatccgct ttttttcgaa acgatacttg taagggtggc agcgaagctt
      301 ttaacgccga ctttgataat accgttgtca atataatttg tcatataatn nnnnnnnnnt
      361 caactccttt ttgagtgtta attaatatta tcattactga ttgaaaaagt ccataatata
      421 taaaaagacg ctgcaaaaat acagcgtctt tttaggagcg ttttaaatta acttattccg
      481 cagcggatat ttccttatcc tttggaagga ttgagttggt aattataccg aggataagcg
      541 cacaagcgat ggaagtgagc gttacgctgc cgaagtttat tgtgagtccg cctatgccgg
      601 ctatgagaat gactgctaca acaaaaaggt tgcggttttc gtcaaggttt accttctgta
      661 tcatcttaag accgcttacg gcaatgaaac cgtaaagagt aatgcaaacg ccgcccatta
      721 cacagttggg tatggaggca agaaatgtaa caaacgggcc gaagaatgaa atgataatcg
      781 ccattatagc agtagcaaga attgtaacaa cagaagcgtt gccggtaatt gcgacgcaac
      841 caaccgattc accatatgtt gtgttcgggc agccgccgaa aacagcacct accattgaac
      901 ctacgccgtc accgagaaga gttctgtgaa ggccgggctc ctcaagaagg tctttgttaa
      961 taatggatga aaggtttttg tggtcggcga tatgctcggc aaaaacaaca aatgcaaccg
     1021 gaacataagc tacggcgatg gtggctacat actggccggt tatttctttt gttcctttaa
     1081 gagcctcaat aaatgtgaag tccggaacag caaagagctt catatttgaa aatacactga
     1141 aatcaattat ttgaagagcg gtattgtttg tttttatgcc gatagcggta aatatggcag
     1201 cggcaacata gcctgcgaga atgcctatga tgaaggggat gagcttcatc att
//
LOCUS       DBMDVO010000507         1252 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51518_length_1252_cov_0.867941, whole genome shotgun sequence.
ACCESSION   DBMDVO010000507 DBMDVO010000000
VERSION     DBMDVO010000507.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51518_length_1252_cov_0.867941"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="leuB"
                     /locus_tag="ACZPE2_06995"
     CDS             complement(<1..736)
                     /gene="leuB"
                     /locus_tag="ACZPE2_06995"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1319630.1"
                     /translation="MGNYNITVLKGDGIGPDIVAEAMNVLEAVGKKYNHKFNFNEKLV
                     GGAALDACGVPLPDDTLEACKNCDSILLGAVGGPKWDNLPSNMRPEAGALLRLRKELG
                     LYANLRPAIIHKALSSASPIKPEIIGDSLDILVVRELTGGIYFGERGHREGKYGEEAY
                     DVEAYSEFEVKRIAKTAFEAAMKRNKRVTSIDKSNVLESSKLWRKAVIEVAKDYPEVE
                     LDHMYVDNGAMQLVRNPKQFDVIVTSN"
     gene            complement(815..>1252)
                     /locus_tag="ACZPE2_07000"
     CDS             complement(815..>1252)
                     /locus_tag="ACZPE2_07000"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1319631.1"
                     /translation="IPARYLNVSSGEELAKYCMIDIDKDFVNNVHKGDIIVAEKNFGC
                     GSSREHAPLAIKCASVSCVIASTFARIFYRNAINIGLPILECDEAARDIKAGDEVSVD
                     FNTGVITNVTTGKSYKAEPFPEFMQKIMAAGGLVSYTSSKISE"
BASE COUNT          311 a          307 c          215 g          419 t
ORIGIN      
        1 tgtttgacgt aactataaca tcaaactgct ttggatttcg tacaagctgc atggcgccat
       61 tatcaacata catatggtca agctctactt cgggataatc cttagcaacc tctataacag
      121 cttttctcca gagcttactg ctttcaagaa cattggattt atcaatgctt gttactctct
      181 tatttctctt cattgccgcc tcgaaggccg ttttggcaat tctttttacc tcaaattcgc
      241 tgtatgcctc aacatcataa gcctcttcgc cgtatttgcc ttctctgtga cctctttctc
      301 caaaataaat tcctcctgta agctctctta caacaagaat atcaaggctg tcaccgatta
      361 tttccggctt aatcgggctt gctgagctga gtgccttatg tataatagcc ggtctgagat
      421 ttgcataaag accaagctct tttctgagtc tgagcaatgc tccggcttcg ggacgcatat
      481 ttgaaggaag gttatcccat ttaggaccgc ctaccgcgcc gagcaaaatc gaatcgcagt
      541 ttttgcaggc ctcaagtgta tcatcgggaa gaggcactcc acaagcatcc agtgccgctc
      601 cgccgacaag cttttcatta aaattgaatt tatgattata tttctttcct actgcctcaa
      661 gaacattcat cgcctccgca actatatccg ggcctattcc gtcgccttta agtaccgtaa
      721 tattataatt tcccatgtca gttcctccaa tcttatttac tattttattt tatcacttgt
      781 cctgtttttt cctccggcaa gttttattta gtatttattc acttatttta ctgcttgtat
      841 agctaacgag gccgccggca gccattatct tctgcataaa ttcagggaac ggctctgcct
      901 tatagctttt tcctgttgtg acatttgtta taacgcctgt gttgaaatca accgaaacct
      961 catctccggc cttaatgtcc cttgcggctt cgtcacattc aagaatagga agtccgatat
     1021 tgattgcatt tctgtaaaat attcttgcaa aggttgatgc aattacacag gatacactgg
     1081 cacacttgat tgcaagcgga gcatgctctc ttgatgagcc acagccgaaa tttttctctg
     1141 caacaattat atcaccttta tgtacattat taacgaaatc cttatctata tctatcatgc
     1201 agtactttgc aagctcttca ccacttgata cattgaggta tcttgcggga at
//
LOCUS       DBMDVO010000508         1251 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51544_length_1251_cov_1.743478, whole genome shotgun sequence.
ACCESSION   DBMDVO010000508 DBMDVO010000000
VERSION     DBMDVO010000508.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51544_length_1251_cov_1.743478"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="ACZPE2_07005"
     CDS             <1..822
                     /locus_tag="ACZPE2_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319632.1"
                     /translation="KGKKALDSFTLTLEKGVYGILGPNGAGKSTLINCVTGIIKPDSG
                     SIAFTDGDSHDYMSHLGFLPQNQDFYPNFTARELILYSMALKNVKVDNPKAYADGILK
                     RVNLYDDRNKKVGAHSGGMKQRLGIAQALVGEPKVVIFDEPTAGLDPKERIRFRNVIS
                     SLANDKIVILATHIVTDIAFIAKNVVLIDGGKMISCASQSELCNEIYGKVWEVLTDYE
                     TGMELMRTKRVSNVISEDDRFNVRVVSDEKPHHDAVNVQPALEDVCIYHFGEIGE"
     gene            824..>1251
                     /locus_tag="ACZPE2_07010"
     CDS             824..>1251
                     /locus_tag="ACZPE2_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319633.1"
                     /translation="MLKYELKKYILKPSVIICLAVLLLVNLVKVLEIYCQGSGRQILF
                     SGKGRIEISKDVLYEKYGGEITQEKIESLKAELAVAEQKLEEYGIIEEPIENTYTGYP
                     YGDVNLIKDIISSYEYALLYTNTSNELSDRAAEKAAFYEG"
BASE COUNT          390 a          255 c          299 g          307 t
ORIGIN      
        1 aaaggcaaaa aagccctcga cagctttacc ctcacccttg aaaaaggcgt gtacggcatc
       61 ttaggtccca acggtgcggg caaatccacg ttgataaact gcgtaaccgg tataataaag
      121 cctgacagtg gcagtattgc tttcaccgac ggcgacagcc acgattatat gtcgcacctc
      181 ggctttttac cgcagaatca ggacttttat cctaatttta cggcaagaga gcttatcctc
      241 tactctatgg cactgaaaaa tgtaaaggtc gataatccca aagcctatgc cgacgggatt
      301 ttaaagcgtg taaatctgta tgacgaccgc aacaaaaagg ttggcgcaca ttcgggcggt
      361 atgaagcaaa ggctcggcat agcgcaggca cttgtaggcg agccaaaggt ggttatattt
      421 gacgagccga cagcaggact tgaccccaag gagcgtatac gcttcagaaa tgtaatctca
      481 tcgcttgcga atgataagat agtaatttta gcaactcata tcgttaccga tattgctttt
      541 attgccaaga atgttgtact tatcgatggc ggaaagatga taagctgtgc ctcacaaagt
      601 gagctttgca atgaaattta cggcaaggtc tgggaggtgc tcaccgacta cgaaacaggt
      661 atggagctta tgcggacaaa gcgcgtcagc aatgttataa gcgaggacga caggttcaat
      721 gtccgtgttg taagtgacga gaagccacac cacgacgccg ttaatgttca gcctgccctt
      781 gaagatgtgt gcatttatca cttcggagag ataggtgaat gatatgctga aatacgagct
      841 taaaaagtac atactgaagc cgtctgtaat aatctgcctt gctgttttat tacttgtaaa
      901 ccttgtcaaa gtgctggaga tttattgtca gggttcgggc agacaaattc tgttttcggg
      961 aaaaggcagg attgaaataa gtaaagatgt tctttatgaa aaatacggcg gcgagataac
     1021 acaggaaaaa atcgaaagcc ttaaagcaga actggctgtt gccgaacaaa agctcgaaga
     1081 atacggtatc atagaagaac cgatcgaaaa tacatacacg ggttatccgt acggagatgt
     1141 caacttaatc aaagatatta tttcttcata cgaatacgca ttgctttaca ctaatacatc
     1201 aaatgagctg tcagacagag cggcggaaaa agccgcattt tatgaaggca g
//
LOCUS       DBMDVO010000509         1250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51604_length_1250_cov_1.342907, whole genome shotgun sequence.
ACCESSION   DBMDVO010000509 DBMDVO010000000
VERSION     DBMDVO010000509.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51604_length_1250_cov_1.342907"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            314..>1250
                     /locus_tag="ACZPE2_07015"
     CDS             314..>1250
                     /locus_tag="ACZPE2_07015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1319634.1"
                     /translation="MEITPKLSVTEDKIRLLTVKTDMFKTACLTLNVLVPMGEYAAEY
                     AVLSSYLVHSSKKYDSLIKLNSRLEELYGALLSGSISRHGENYRIQLSITCIDDSLTF
                     DGKSVTGASLELLLSLLTKPSADENGFSPEQLSTEIRLTAEDIESEINDKRSYAMTRM
                     LETMCADEIYGLTKNEILENVKKVTPQSLLAAWKFMLKNGIIQLNAIGNISEDDCKKK
                     LREAFSDVGERTPYEPETVFVEEAEDLTELTEELTMNQSKLVLGFRGGMTDENDDFYS
                     RRIMTDVFGGGPYSRLFMNEREKLSLCYYCSARLIR"
BASE COUNT          359 a          257 c          300 g          334 t
ORIGIN      
        1 gccgatttaa tacactttat cacgcgtttg tttatttttt aacaatgtct gcttgaacat
       61 cataagccgc acgtgtttaa tgtgattatt aacttcaaaa agccgatttt gtttgccgcc
      121 gcgaaaaaaa taaaaatact gcggattttt gggattacgg taattaaatt ttttataaag
      181 ctgtggaatt ttcgccactt atatgataca ataataaata attataggct ttaaaagccg
      241 tgccttgccg cgagaaaaac agcactcggg gcgaggtgct ttcaaaaaac tatttcggag
      301 gcgttctcgc aatatggaaa ttactccaaa gctttctgtt accgaagata aaataaggct
      361 tttgaccgta aaaaccgata tgttcaaaac ggcgtgcctt acgcttaacg tattggtgcc
      421 gatgggtgaa tatgcggcag aatatgccgt gctgtcatcg tatttggtgc attcgtcaaa
      481 aaaatacgat tcgcttataa agctgaactc acgcttggag gagctttacg gcgcactttt
      541 gtcgggcagc atttcaagac acggcgagaa ttatcgtata cagctttcga taacctgcat
      601 agacgacagt ctcacctttg acggcaagag cgtcacgggc gcgtcgctgg agttgctttt
      661 gtcgcttctt acaaaaccgt cggcggacga aaacggattt tcaccggagc agctgtcaac
      721 ggaaatacgt cttaccgcag aggatattga gagtgaaatc aacgataaac gttcatatgc
      781 gatgactcgt atgctcgaga cgatgtgtgc cgacgaaatc tacggactta ccaaaaatga
      841 aattctcgaa aacgtgaaaa aggtcactcc gcaatcgctg cttgccgcgt ggaaatttat
      901 gctgaaaaac ggcataattc agctgaacgc cataggcaat atttcggagg acgattgtaa
      961 aaagaaatta cgcgaggctt tttcggacgt cggcgagaga acgccgtatg agccggagac
     1021 cgtttttgtc gaagaggcgg aggatttaac cgagcttacc gaggagctta ccatgaatca
     1081 gagcaagctt gttctcggct tcagaggcgg tatgaccgat gaaaacgacg acttttattc
     1141 gcgccggatt atgacggacg ttttcggcgg cggcccgtat tcgagacttt ttatgaatga
     1201 acgcgaaaag ctcagccttt gctattattg ctcggcaagg cttataagag
//
LOCUS       DBMDVO010000510         1250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51616_length_1250_cov_1.153177, whole genome shotgun sequence.
ACCESSION   DBMDVO010000510 DBMDVO010000000
VERSION     DBMDVO010000510.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51616_length_1250_cov_1.153177"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="ACZPE2_07020"
     CDS             <1..1119
                     /locus_tag="ACZPE2_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HIR1319635.1"
                     /translation="SFTELADEQKSEKEEVYLYADFLSKLFAETDNFSKYVLNAVLED
                     ENAYILKYGHKKLGSHYDEALNMELDTLNELAAVRSDDVKKSLRLENESLPGWTTEKT
                     DVKSIYLFRIKNISKTGYGIWAKYHVFIIKNGDIVPVKYPDTQKLSDFSGYERERSEV
                     IDNTKALLKGEPCNNVLLYGDAGSGKSSTVKAIANEFKDDGLRLIEVKKNQLYSLPDI
                     MDRISGNPLKFIIFIDDLSFTKNDEDFGALKAILEGSVNGRAKNIAIYATSNRRHLVK
                     ESFADRDGDDVHIQDTMQELTSLSARFGLQITFSRPDKNLYSGIVVELAKKYGINMPE
                     DKLIMKAEAHALRAGGRSPRTARQFIEYLAYKQDAEKE"
BASE COUNT          416 a          248 c          296 g          290 t
ORIGIN      
        1 tcgttcaccg agcttgccga tgaacaaaag agcgaaaaag aagaggttta cttatatgct
       61 gattttctct cgaagctgtt tgcggaaacc gataattttt caaaatatgt tttaaacgcc
      121 gttcttgagg acgaaaacgc ttacatatta aagtacggtc acaaaaagct cggcagccat
      181 tatgacgagg ctctgaatat ggagcttgac acgcttaacg aactggcggc cgtgcggtcg
      241 gacgatgtta aaaagagcct gcgcctcgaa aatgagtctc tacccggctg gactaccgag
      301 aaaaccgatg taaaaagcat ttatctcttc agaataaaaa acatatccaa aaccggatac
      361 ggcatatggg caaaatacca cgtctttatc attaaaaacg gtgatattgt gcctgttaaa
      421 taccccgaca cgcaaaaact ttcggatttt tccggttacg agcgcgagcg cagcgaggta
      481 attgataaca caaaggctct tttaaaggga gaaccgtgca ataatgttct tttatacggc
      541 gatgccggaa gcggtaagag cagtaccgta aaagcaatag cgaacgaatt taaggatgac
      601 ggtttgagac ttatcgaggt taagaaaaat cagctctatt ccctgccgga cataatggac
      661 agaatatcgg gcaatccgct taaattcata atatttatag atgatttgag cttcaccaaa
      721 aacgatgagg acttcggtgc gctgaaggct attttggagg gcagcgtaaa cggcagggcc
      781 aaaaacatag cgatatacgc aacatcaaac cgccgccatc tcgtcaaaga gagctttgcc
      841 gaccgtgacg gagacgatgt tcatattcag gatacgatgc aggagcttac aagcctttcg
      901 gcaagattcg gcttgcaaat cacgttttca aggccggaca aaaatctgta ttcgggcatt
      961 gttgttgaac ttgcaaaaaa atacggcata aatatgccgg aggacaagct tataatgaaa
     1021 gcggaggctc acgctttgag agccggcggc agaagtccga ggaccgcaag gcaatttata
     1081 gaatatctcg catacaagca ggatgcggaa aaggaataag ctgttattaa tttgtttgta
     1141 taaattcagt ataaaaagat tggagaagaa aatggagatc aaaaaaagat acgattggga
     1201 aaatcccgcc gttatcaaaa gaaacaaaga ggacggccac gttatcgcat
//
LOCUS       DBMDVO010000511         1250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51630_length_1250_cov_0.970409, whole genome shotgun sequence.
ACCESSION   DBMDVO010000511 DBMDVO010000000
VERSION     DBMDVO010000511.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51630_length_1250_cov_0.970409"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(216..395)
                     /locus_tag="ACZPE2_07025"
     CDS             complement(216..395)
                     /locus_tag="ACZPE2_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319636.1"
                     /translation="MKKVLKVIGIIAAVAGAIFGVYVIVKKYVDSKKVVIGNDAENYV
                     SCSCCDDNFVSETVA"
     gene            complement(538..795)
                     /gene="rpsT"
                     /locus_tag="ACZPE2_07030"
     CDS             complement(538..795)
                     /gene="rpsT"
                     /locus_tag="ACZPE2_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1319637.1"
                     /translation="MPNIKSAKKRVIVNNKRADRNKSRNTALKTAIKKANAAIESNAA
                     DKEVLVKAAVKKIDQASAKGLIHMNNAARKKSALVCKLNKA"
     gene            1000..>1250
                     /locus_tag="ACZPE2_07035"
     CDS             1000..>1250
                     /locus_tag="ACZPE2_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319638.1"
                     /translation="MIFGTDLAIERREITNSGKNDGVTVKKRRIKSASVTEIEITTDA
                     GAEKLGKPVGTYVTVELPPFSSEFDDADSRMFAVRDESK"
BASE COUNT          399 a          240 c          256 g          355 t
ORIGIN      
        1 tcattggttt cctgccttcg gtataaaaag atttttacgg ctgagaaact gtaaagcact
       61 ttaaaatatt atatttttga tacaagaaaa tgaaaacccc tcagataagc cggagctcat
      121 ctgaggggtc aattattatt gcacaaaaaa tattgttttg acgcaaagct tccttataaa
      181 cactcagcaa tcgggcgttt ataatacgtt tttatttacg caacggtttc ggaaacgaag
      241 ttgtcgtcgc agcaggagca tgaaacataa ttttcagcgt cattgccgat aactactttt
      301 ttgctgtcaa catatttttt cactattaca taaacgccga agatagcgcc tgcaacagcg
      361 gcgatgatac cgataacttt aagaactttt ttcataggta aaatgcctcc cttaattttt
      421 gtaaaactat gtgtttatta tatacgtaaa aaaggcaaaa gtcaatgaat aaaatgtaaa
      481 tttgaccgaa aaagcaaaac accccggtct aaagaccgag gtgcattaaa aacaacatta
      541 agccttgttg agtttgcaaa cgagggctga ctttttgcga gcggcgttat tcatatgaat
      601 aagacccttt gcagaagcct ggtcgatttt cttaactgca gctttaacaa gaacttcttt
      661 gtcggcagcg tttgactcaa tagcggcatt agcctttttg atagctgtct tgagagcggt
      721 gtttcttgac ttgttgcggt ctgctctctt gttgttaacg attacacgtt ttttagctga
      781 tttaatattc ggcatagttt aacctccttt gcgtatcaca ttagcggaat atataatacc
      841 attctttccg aaaaaaagca agtttttttt tcataaacag cctataaaat ttatgccggt
      901 gcaaataata tatcgtaaag cgcgcccgaa attcgcaaaa tgcggcaaaa cgggctgcag
      961 cacacaaaca tttatcgaaa gaaaggcagc agtatttata tgattttcgg aaccgatctc
     1021 gcaattgagc gcagagagat aacaaacagc ggcaaaaatg acggcgtgac ggtaaaaaag
     1081 cgaaggataa aaagcgcgtc ggtcaccgaa atagaaatta caacagatgc cggtgccgaa
     1141 aagctcggca aacccgtcgg cacatatgtc acggtggagc tgccgccgtt ttcatcggaa
     1201 tttgatgatg cagacagcag aatgtttgcc gtgagggatg aaagtaaaaa
//
LOCUS       DBMDVO010000512         1245 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51900_length_1245_cov_0.873252, whole genome shotgun sequence.
ACCESSION   DBMDVO010000512 DBMDVO010000000
VERSION     DBMDVO010000512.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51900_length_1245_cov_0.873252"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..631
                     /locus_tag="ACZPE2_07040"
     CDS             83..631
                     /locus_tag="ACZPE2_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HIR1319639.1"
                     /translation="MLIKNGKPWDALFDIGLWYLLLIGLVLFGVGGSVAAGLATAGKW
                     MSIVGAVGILVTGGRNKKGFGKITGGLGSLYGITSYLSDVLSYSRLLALGLATGVVAK
                     VVNTLGSLAGNGIGGLIVFIIVFLIGNVFNIAINALGAFVHSCRLQYVEFFGKFYTGG
                     GRPFEPFAGKTKFVKITNKEEK"
     gene            633..1118
                     /locus_tag="ACZPE2_07045"
     CDS             633..1118
                     /locus_tag="ACZPE2_07045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIR1319640.1"
                     /translation="MTESIFSGEFLACLGAAMAAIAGIGSGIGVGVAGEAAAGVVSED
                     PKKFGKVLLLQALPGTQGIYGLLIAFIILVKIGLLGGDGMVDLTVAQGASIFAAAIPI
                     GLVGIFSGIAQGKAAAAGIMLVGKNPSELSKGMLFAAMVETYAVLALLISFLMLNSIK
                     L"
BASE COUNT          336 a          235 c          305 g          369 t
ORIGIN      
        1 atctttcgat tgcgcagcat ccactggcac gttataataa taccataaag ttaattttgg
       61 cattccaaag gaggattttt atatgcttat caaaaacggc aagccctggg atgcgttgtt
      121 tgatataggt ctttggtatc tgcttcttat cggccttgtg cttttcggag taggcggatc
      181 ggttgcggca ggacttgcaa cagcaggtaa atggatgtcg atagtcggtg cggtcggaat
      241 acttgttacc ggcggacgta acaaaaaagg ctttggaaag atcacaggcg gtcttggtag
      301 cctttacgga ataacaagtt acctttcgga tgttctttca tattcaagac ttcttgctct
      361 cggtcttgcg accggagttg tagcaaaggt tgtaaataca ctcggttcac ttgccggaaa
      421 cggtatcggc ggacttatag tatttataat cgtattcctg ataggtaacg tatttaatat
      481 tgcgataaat gctctcggag cattcgtaca ttcttgcaga cttcagtatg ttgagttttt
      541 cggaaaattc tatacaggcg gcggcagacc gtttgaacct tttgccggaa aaacaaaatt
      601 tgtgaagatt acaaataagg aggaaaaata atatgacaga gagtatattc agtggtgaat
      661 ttttagcatg tttaggcgct gcaatggcag ctatagcagg tatcggaagc ggtatcggcg
      721 taggtgttgc cggtgaggca gcagcaggtg tagtttccga ggatccaaaa aaattcggta
      781 aggttcttct tcttcaggct cttcccggaa cacagggtat ctacggactt cttattgcat
      841 tcatcattct tgtaaaaatc ggtcttctcg gcggtgacgg tatggttgac cttactgttg
      901 cacagggtgc ttcaatattt gcggcagcta ttcccatcgg tcttgtcggc atcttctcag
      961 gtattgcaca gggcaaggca gcagcagcag gtatcatgct tgtaggcaaa aatccttcag
     1021 agctttcaaa gggtatgctt ttcgcagcta tggttgaaac atatgccgta cttgcactcc
     1081 ttatttcatt ccttatgctt aacagcatca agttataatt aatagaagag agggaggata
     1141 ctcctatgaa tgatggcaaa attattattg acaaaattat agccgatgct gaagaagcag
     1201 ttaataaaac cattgcagag gcaaaggctg ccgctgatga aatac
//
LOCUS       DBMDVO010000513         1244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51953_length_1244_cov_1.067367, whole genome shotgun sequence.
ACCESSION   DBMDVO010000513 DBMDVO010000000
VERSION     DBMDVO010000513.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51953_length_1244_cov_1.067367"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1244
                     /gene="rlmD"
                     /locus_tag="ACZPE2_07050"
     CDS             <1..>1244
                     /gene="rlmD"
                     /locus_tag="ACZPE2_07050"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1319641.1"
                     /translation="KKKSISNNKSGVKSGNCPLYKKCGGCQLQNLTYEQQLRYKQVKC
                     IRLLGKFCRVGEIIGMENPYHYRNKVQAAFALDRHSNIISGVYQSSSHKIVPVTVCMT
                     EDEKADEIIGTIRRLLKNFKLRPYNEDTGRGFLRHVLVKRGFKSGQIMVVLVTGTRDF
                     PKKKDFVATLLKIHPEITTVVQNVNNQKTSMVLGNRSEVLFGDGYITEQLGDFSFRIS
                     PKAFYQINPIQTEVLYNTTLEFAGLTGKETVIDAYCGTGTIGIFASPKAKKVVGVELN
                     PDAVKDARVNAKLNSAENTEFYNADAGEFLADAASSNEKYDVVIMDPPRSGSTVKFLK
                     SVAKLAPKTVVYVSCNPETLARDLMFLVRNGYKVKKIQPVDMFPHTNHVETVVLLSHK
                     KADSYIHIDVEFGEGEGKIPVD"
BASE COUNT          373 a          271 c          311 g          289 t
ORIGIN      
        1 aagaaaaagt caatcagcaa caataaaagc ggcgttaaaa gcggcaattg ccctctttac
       61 aaaaaatgcg gcggatgcca gctccaaaat ttaacatatg agcagcagtt acgatacaag
      121 caggtaaagt gcataaggct gctcggaaaa ttctgccgcg tcggcgagat cataggtatg
      181 gaaaacccct atcactatcg aaacaaggtt caggcggctt tcgctcttga cagacacagc
      241 aatataatat cgggagtcta tcagtcatcg tcgcacaaaa tagttcccgt caccgtttgt
      301 atgaccgagg acgaaaaagc tgatgagata ataggcacga ttcgccggct tttgaaaaac
      361 tttaagcttc gcccttataa tgaggatacg ggcagggggt ttttgcgcca tgtgcttgta
      421 aagcgcggct ttaaaagcgg ccaaataatg gtggtgctcg tcaccggaac aagggacttc
      481 ccgaagaaaa aggactttgt tgcgacactt ttgaaaatac atcccgaaat taccaccgta
      541 gtgcaaaacg taaacaatca aaaaacaagt atggttctcg gcaacaggag cgaggtgctt
      601 ttcggcgacg gatatataac ggagcagctc ggcgattttt cgtttagaat atctccgaag
      661 gctttttatc agataaatcc catacagacc gaggtgctgt ataatacgac gcttgaattt
      721 gccgggctta ccggtaagga aaccgttatt gacgcatatt gcggcaccgg aacgataggc
      781 attttcgctt cgccgaaggc taaaaaagtg gtgggtgtgg agcttaatcc cgacgccgta
      841 aaggacgcac gcgtaaacgc aaagctcaac tccgccgaaa atacggagtt ttacaatgcg
      901 gacgccggcg agttcttggc ggatgccgct tcttcaaacg agaaatacga tgttgttata
      961 atggaccctc cgcgcagcgg aagcacggtg aagttcttga aatcggttgc aaagctcgca
     1021 ccgaaaaccg ttgtgtatgt gtcctgtaac cccgaaactc ttgcaagaga tttgatgttt
     1081 cttgtcagaa acggctataa ggtcaaaaaa atacagcccg tcgatatgtt tccgcataca
     1141 aatcatgtgg agacggtagt tcttttgtcc cacaaaaaag ccgacagtta tatccacatt
     1201 gatgtggagt ttggagaggg cgagggcaag attccggtag atag
//
LOCUS       DBMDVO010000514         1243 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_51990_length_1243_cov_1.355517, whole genome shotgun sequence.
ACCESSION   DBMDVO010000514 DBMDVO010000000
VERSION     DBMDVO010000514.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51990_length_1243_cov_1.355517"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1243)
                     /locus_tag="ACZPE2_07055"
     CDS             complement(<1..>1243)
                     /locus_tag="ACZPE2_07055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1319642.1"
                     /translation="KQIKEDKRTDIVLLSGDTTNNGEIEAHEEFIEMLRDLKKSGKRV
                     YVLTATHDYQDDGLTDSFVGNEKVKIPAAKREQLYDMYKEFGPDEAIAVHRDSMSYVV
                     QLADGYRLFALNDDRNLSGKSGFSDECFEWIKAQAEDARKNDQFILAMTHHPLIAPSP
                     IYELIGKNDMLGDYETRRNELADLGIQFILTGHTHVHDIDVITSDRGNTLYDIATAAT
                     VGYPAPIRTIVFDPDVKMVSTTTDLITETVDFDLEGKTLQEYLKYQLIGMVKDMIKAA
                     GTDIPTLADMATAMSIKKKLIYKIGWLIKPFAKKLNALTIGKVAKLTRAETGLKPEDY
                     ADIADKSVVDFICDLVVNLYGGEDLYSVDDNEYKITVGLLHIVDSVFAALHIKPRKLI
                     KVADSFTDFAEPLLHNSGIPSY"
BASE COUNT          307 a          296 c          275 g          365 t
ORIGIN      
        1 atatgacggt atgcccgaat tatgtaaaag cggttccgca aaatcggtaa acgaatcagc
       61 aacctttata agcttgcgcg gctttatatg aagagcggca aaaaccgaat ctacaatgtg
      121 aagaagccct acggttattt tatattcgtt atcatcaaca ctgtaaaggt cttctccgcc
      181 gtacaaatta acaacaaggt cacagataaa gtcaacaacg cttttatcgg caatatcggc
      241 ataatcctcg ggcttcagcc ctgtttcggc acgggtcagc tttgcgactt tacctattgt
      301 aagagcatta agctttttgg caaacggttt tataagccag ccgattttat aaatgagttt
      361 tttctttatg ctcattgccg tcgccatatc ggcaagcgtc ggaatatccg ttccggcggc
      421 ttttatcata tcctttacca taccgataag ctgatatttg agatactcct gaagggtttt
      481 gccctccaaa tcgaagtcca ccgtttcggt tatgaggtcg gtggtggttg aaaccatttt
      541 tacatccggg tcaaaaacaa tggtgcgtat cggtgcggga taacctacgg ttgcggcggt
      601 tgctatatcg taaagcgtat ttccgcggtc gctcgttatc acgtctatat cgtgtacgtg
      661 agtgtgaccc gtaagaataa actgtatgcc taggtcggca agctcgtttc tgcgcgtctc
      721 gtagtcgccg agcatatcgt ttttgcctat cagctcataa atcggcgacg gggctataag
      781 cggatggtga gtcatagcca aaataaactg gtcgtttttg cgtgcatcct ccgcctgagc
      841 ctttatccac tcaaagcact catccgaaaa gccgcttttt cccgagagat tgcggtcgtc
      901 attaagtgca aaaagcctgt aaccgtcggc aagctgcact acatacgaca tactgtctcg
      961 atgaacagca atcgcttcat cggggccgaa ctctttatac atatcataga gctgctcacg
     1021 ctttgcggca gggattttca ccttttcatt gcctacaaag ctgtctgtca agccgtcatc
     1081 ctgatagtcg tgggttgccg taagaacata aacgcgcttt ccgctctttt ttaaatcgcg
     1141 gagcatttcg ataaattcct cgtgtgcctc aatttcaccg ttgttggtgg tatcgcccga
     1201 aagcaaaacg atatcggttc gtttatcttc ttttatctgc tta
//
LOCUS       DBMDVO010000515         1242 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52051_length_1242_cov_1.273444, whole genome shotgun sequence.
ACCESSION   DBMDVO010000515 DBMDVO010000000
VERSION     DBMDVO010000515.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52051_length_1242_cov_1.273444"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="ACZPE2_07060"
     CDS             complement(<1..989)
                     /locus_tag="ACZPE2_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012360.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319643.1"
                     /translation="MALKTGALIIAADNKKREKPIYMCEALSLPLISYVKSYAEKADA
                     EKTAIIIESDSSGVEAIKGDSRVFVSPDAENDSDFLLAADGFADEFDYVYVLYGNVPL
                     MNGSSLKNALSLCVNEGNEAAAVFSRQPNGEDVTGAYVFSSKKLMTLIKNGASRSAEE
                     LFRACDKKARFQTDCRCETSAVYDMCSLHEISETVRLREIEHQLDCGVNIPCFDGVMI
                     SPNVKIGEGTLILPGTILRGNVTIGKNCTVGPNTLLHNTTVGDDAYLNSVQSFDARIM
                     SGVNIGPFVHIRPNSVIGEKVHLGNFVEVKNSNIDTGTKVSHLTYVGDSDVGK"
BASE COUNT          316 a          310 c          247 g          369 t
ORIGIN      
        1 ctcttgccca cgtcgctgtc gccgacatac gtaaggtgcg aaacctttgt gccggtatct
       61 atattggaat ttttcacctc gacgaagttg ccgagatgca ccttttcgcc tatcaccgaa
      121 ttgggtctga tatgcacaaa cgggcctata tttacgccgc tcattatcct tgcgtcaaag
      181 ctttgaacgc tgtttaaata cgcatcatcg ccgacagttg tattgtgaag aagcgtattg
      241 ggccctacgg tgcagttttt gcctatcgtt acgtttccgc gtaaaatcgt gccgggcaaa
      301 ataagcgtac cctcgccgat ttttacgttc ggggaaatca taactccgtc aaagcacggg
      361 atattcaccc cgcagtcaag ctgatgctct atctctctta agcgaacagt ctcgctgatt
      421 tcgtgaagtg agcacatatc atagacggca ctcgtttcgc atctgcaatc ggtttggaac
      481 cgtgcttttt tatcacaggc gcgaaaaagc tcctcggcag agcgagatgc gccgttttta
      541 atgagtgtca tgagtttttt tgaagagaac acatacgctc cggtcacgtc ctcaccatta
      601 ggctgtcttg aaaagactgc ggccgcctca tttccctcgt tcacgcagag agaaagcgcg
      661 tttttaagag agctgccgtt cataagcggt acatttccat agagaacata tacatagtca
      721 aattcgtctg caaagccgtc ggctgcaagc agaaaatcgc tgtcattttc ggcatccggc
      781 gacacaaaca cccggctgtc gccctttatt gcttcaacac cggagctatc actttcaatt
      841 ataattgccg ttttttccgc atcagccttt tcggcatacg acttcacata cgaaatgagc
      901 ggcaaagaca aagcctcaca catataaatg ggtttttcgc gttttttatt atctgcggct
      961 ataatcagcg cacctgtttt aagagccata agagcactct cccaatatat tttgtattct
     1021 gccgccgtat ctgcccgata cctttgcagc aacaatatta ttgtatcaca aaatgtcttt
     1081 tcttacaata aaaacatccg cctcattgcg cacaacaaat caacacttcg gcccgacttg
     1141 ttagttttta ccaatataag cagtttaatt ttgtagtgtt ttttcgaaaa aagctctttt
     1201 ctttttttat tttctctgat atattataca caggaaggat ac
//
LOCUS       DBMDVO010000516         1241 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52106_length_1241_cov_1.437719, whole genome shotgun sequence.
ACCESSION   DBMDVO010000516 DBMDVO010000000
VERSION     DBMDVO010000516.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52106_length_1241_cov_1.437719"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            33..303
                     /locus_tag="ACZPE2_07065"
                     /pseudo
     CDS             33..303
                     /locus_tag="ACZPE2_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015280.6"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
     assembly_gap    304..313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <314..799
                     /locus_tag="ACZPE2_07070"
     CDS             <314..799
                     /locus_tag="ACZPE2_07070"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1319644.1"
                     /translation="GLNILIDNPAELGADLVAGAVGAMARYEMPAFVIDLGTATKIYA
                     VDEHGGYHGGTIAPGVEISMKALSGQASLLPSFSLASPPHACATNTIECLQSGIIFGT
                     ADMIDGMLDRFAKQLGDPKTIVATGGLSSYVIGYCRHNIIHDDNLLLYGLKAIYDKNS
                     N"
     gene            895..>1241
                     /locus_tag="ACZPE2_07075"
     CDS             895..>1241
                     /locus_tag="ACZPE2_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1319645.1"
                     /translation="MSKTTNKARMQKTVRLVELALLTAIIAVMTFTPIGYLKLGPLEL
                     TLIMVPVIIGAVTLGPAAGAFLGLVFGASSFIQCFSLSAFGAILISISVFKTLVVCVV
                     PRVLAGWLTGLIF"
BASE COUNT          303 a          264 c          286 g          378 t
ORIGIN      
        1 ggaactgcac attttgtgct tttgcttaat ttatgatact gacgatcgat atcggtaata
       61 ctaatattac cttcggagtg tttgacggcg ataagctgag ctttgtttcg cgacttgcta
      121 ccgagcgtaa cagaactgac gatcagtttg ccgttgaatt tcttgccgtg tttaaaatgc
      181 atgatttgga gccggctgaa attaccggag ctgttttaag ctctgttgtt cctgagctta
      241 ccgggaaaat taaaacagcg gttaataagg tgtgcggcat tgagtctgtt ataatagccc
      301 ccgnnnnnnn nnngggctta atattttaat agataatccg gcagagctcg gtgccgacct
      361 tgtagcaggt gcggtaggag ctatggcgag atatgaaatg cccgcatttg tcattgacct
      421 cggcacagcc acaaaaatat atgcagttga tgagcatggc ggctatcacg gcggcacaat
      481 agcaccgggt gttgaaatct ctatgaaagc actttcgggg caggcttctc tcttaccgtc
      541 attttcactt gcttctccgc cgcatgcctg tgccactaat acgatagagt gtttgcagtc
      601 gggcatcatt ttcggcaccg cagatatgat tgacggtatg cttgaccgtt ttgcaaaaca
      661 gctcggtgat ccgaaaacca ttgttgcaac aggtgggctt tcctcctatg taataggata
      721 ttgccgtcac aatataattc atgatgacaa tcttctttta tacgggctta aagccattta
      781 tgataaaaac agtaattaat cgtaattaaa aattaaatat cgtatccagt ttttacatag
      841 tttattattt tgcgttgcat ccgctctgcg gagcagcagc aaggaggatt atatatgtca
      901 aaaaccacta acaaggcaag gatgcaaaaa accgttcgct tagtcgagct ggctctcctt
      961 accgcaatca tcgccgttat gaccttcacg ccgataggct atttgaagct cgggccgctt
     1021 gaactgacac ttattatggt acctgttatc ataggcgcag ttactttagg gcctgccgca
     1081 ggagcctttt tggggctcgt tttcggtgca tcgagcttta ttcaatgctt ttctctgagc
     1141 gcattcggcg cgatacttat ttctataagc gtatttaaaa cgcttgttgt ttgcgtagtt
     1201 cctcgcgtgc ttgcaggttg gctcacaggt ctcattttta a
//
LOCUS       DBMDVO010000517         1240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52206_length_1240_cov_0.730465, whole genome shotgun sequence.
ACCESSION   DBMDVO010000517 DBMDVO010000000
VERSION     DBMDVO010000517.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52206_length_1240_cov_0.730465"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1240
                     /locus_tag="ACZPE2_07080"
     CDS             <1..>1240
                     /locus_tag="ACZPE2_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319646.1"
                     /translation="LKSNIAVALIQFFIGALAFVYVPAKVLFKKVAADSASGKIESYI
                     VKNDYSFYLTDNMQYLRYLLIAAFLILGIIMGIVTFKFMTGKKTMNVYYSLGIKRTHL
                     FTAKYLSGLTLMAVAIFLPILADVIMNTVKLGMSSKLLFSALFYFFGLFSLSALSYSL
                     TAMVFSAVGTVFEGVVFSGIIVSMPAIILYCVQSFIIRLVPGTPFGVVFSLSGYSGSE
                     ESIFNKLPWCTPFTYLSKGLYQNMYANAKGKIAGYETGKLVDWTTPDFRSILLWFGIT
                     VVLFFCAMIVYKKRKAEIGGFIGKNNVLNFLCTFTIGFYGFVLAYSFSSTYIGKWPSL
                     AISCLIFAVIYFVLDIIFIRNLKLFAKGLYKLPIHLAAALLIFAFFATGYFGAASKTP
                     KIEKISNIKITSGYTSYSDDA"
BASE COUNT          331 a          222 c          260 g          427 t
ORIGIN      
        1 gtcttaaaag caacattgcc gtagcactta tacagttttt tataggtgcg ttggcatttg
       61 tatatgtacc ggctaaggtg ctgtttaaaa aggtcgccgc tgactctgcg tcaggcaaaa
      121 ttgaaagcta tatcgtaaaa aacgattact ctttttattt gaccgataat atgcagtatc
      181 ttcgttattt acttattgct gcttttttga tacttggtat tataatggga atagtgacgt
      241 ttaagtttat gaccggaaag aagactatga atgtttatta cagtctcggc ataaagcgga
      301 cacatctttt tacagccaag tatctttccg gacttacgct tatggcggtt gcgatttttt
      361 tgccgatatt ggcagatgta ataatgaata ccgtaaagct cggaatgtcc tctaaactct
      421 tgttttccgc acttttttat ttctttggtt tattttcttt gtcagccctt tcatattcgc
      481 ttacggcgat ggttttcagc gcagtcggga cagtttttga gggcgtggta ttttccggta
      541 ttatagtgtc tatgcccgca attattcttt attgcgttca gtcttttatc ataagacttg
      601 tgccgggaac gccgtttggc gtggtttttt cgctgtcggg atattcgggc agcgaagaat
      661 caatatttaa taagcttccg tggtgtacac cgtttaccta tctttccaaa ggcttatatc
      721 aaaatatgta tgccaatgca aaggggaaaa tagccggata cgaaacaggg aaattagttg
      781 attggaccac tccggatttc agaagtatat tattatggtt cggaataacg gttgtattgt
      841 ttttctgcgc tatgattgtt tacaaaaaac gcaaagcgga aataggcggc tttatcggca
      901 aaaacaatgt gcttaatttc ctgtgcacct ttacaatcgg attttacgga tttgtattgg
      961 catacagctt ttcgtcgaca tatatcggca aatggccgtc gcttgcaatc tcttgcttga
     1021 tatttgcagt tatatatttt gtgttggata ttatatttat aagaaaccta aagctttttg
     1081 ccaaaggtct atataagctt ccgatacatc ttgcagcggc tctgttgata tttgcattct
     1141 tcgctaccgg atatttcgga gcagcgagca aaacaccgaa aattgaaaaa ataagcaata
     1201 taaaaataac atcggggtac acaagctact ctgatgatgc
//
LOCUS       DBMDVO010000518         1239 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52242_length_1239_cov_1.050088, whole genome shotgun sequence.
ACCESSION   DBMDVO010000518 DBMDVO010000000
VERSION     DBMDVO010000518.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52242_length_1239_cov_1.050088"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="thrC"
                     /locus_tag="ACZPE2_07085"
     CDS             <1..1176
                     /gene="thrC"
                     /locus_tag="ACZPE2_07085"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1319647.1"
                     /translation="GPTCAFKDMALQLLPYLLSVSASKTLKDTKIVILVATSGDTGKA
                     ALEGFKDVDNTQILVFYPVDGVSPMQKLQMTTQEGNNVSVCAIKGNFDDAQSGVKSIF
                     TNSEIKKKFAENHLAFSSANSINWGRLVPQIVYYVSAYCDMVENGSLKLGEEMNVVVP
                     TGNFGNILAAYYAKRMGVPIGKLICASNSNNVLTDFLKTGTYDKNRNFYCMASPSMDI
                     LISSNLERLLFHLSGENDAEVREDMKLLANSGKYTVGEQLFEKIKELFRAGYCDDTLT
                     KATIKENFDKYHYLCDTHTAVAVKVYEDYVSESGDKTPTVIASTANPYKFSASVLSAL
                     TSDVQSTDEFSMVDELHTLTGEPVPPQLATLKDKKVRFGDVTTKDDMANVVFKMLNI"
BASE COUNT          374 a          231 c          266 g          368 t
ORIGIN      
        1 ggtcccacat gcgcttttaa ggatatggct ttgcaacttt taccgtatct tctttccgta
       61 tctgcttcca agactctcaa ggatactaag attgttattc ttgtcgcaac ctcgggtgac
      121 acaggaaaag cagcacttga gggctttaaa gatgtggata atactcaaat acttgtcttt
      181 taccctgttg acggtgtgag tcctatgcaa aagcttcaga tgactacaca agagggtaat
      241 aatgtaagtg tgtgtgctat caaaggcaat ttcgatgatg cacaaagcgg agttaagtct
      301 atatttacaa attcggaaat taaaaagaaa tttgcagaaa accatcttgc gttttcatcg
      361 gctaactcaa ttaactgggg caggcttgtt cctcagatag tatattatgt atcggcatat
      421 tgtgatatgg tcgagaacgg ttcgctaaag ctcggtgagg aaatgaatgt tgtagttcct
      481 accggaaatt tcggcaacat acttgccgct tattatgcca agagaatggg cgtgccgata
      541 ggtaagctta tttgtgcatc caactctaat aatgtactga cagatttcct taaaacaggt
      601 acatacgaca aaaatcgtaa tttctattgc atggcatctc cgtcgatgga tatacttatt
      661 tcaagtaatc ttgaaaggct tttgttccac ctttcgggtg aaaatgatgc tgaagtaaga
      721 gaagatatga aattgcttgc aaacagcggt aaatatactg taggggaaca gctctttgaa
      781 aaaatcaaag aattattcag agccggctat tgtgatgata ccttgacaaa ggcgactatc
      841 aaagaaaact ttgataaata tcattatctt tgcgatacgc acacggctgt cgctgtcaaa
      901 gtttatgagg attatgtcag cgaaagcggc gataaaacgc ctacggtaat agcgtctact
      961 gcaaatccgt ataaattctc cgcaagtgtt ttgtcagctc ttacaagcga tgttcaatct
     1021 accgacgaat tctcaatggt agatgaattg cacacgctta caggcgaacc cgtaccgccg
     1081 cagcttgcta cgcttaaaga taagaaagta agattcggtg atgtaacgac taaagacgat
     1141 atggcaaatg tagtattcaa aatgctcaac atttaatctg ttcatttccg gatttttgtt
     1201 gtctgaacgg agctttggta tgtcattatt tgctatcgg
//
LOCUS       DBMDVO010000519         1237 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52317_length_1237_cov_1.498239, whole genome shotgun sequence.
ACCESSION   DBMDVO010000519 DBMDVO010000000
VERSION     DBMDVO010000519.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52317_length_1237_cov_1.498239"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="ACZPE2_07090"
     CDS             <1..754
                     /locus_tag="ACZPE2_07090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1319648.1"
                     /translation="CVRRFWKKRPIKDIVEVIGVIAGTAAYYAFLSETVGYLLQHLNA
                     ESYKGSAFLSGIALFLAALFMLFGTLAVKNFSDDSMKKLINFAFAMICTVILANAVVA
                     IVKIPFGRMRYRAMNTAGGASIGGFANFTRWYVRNGQMDKAQMMTLFGTTDACKSFPS
                     GHTCAAGMSYGLIMLADSLGIKSKGKRAALWICPILFTGIVAVSRIVVGAHFFSDVLM
                     GGTISFLSVMLFREIFILKGANLKAVFAKSKD"
     gene            846..1016
                     /locus_tag="ACZPE2_07095"
     CDS             846..1016
                     /locus_tag="ACZPE2_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1319649.1"
                     /translation="MAYKISDDCISCGACAAECPVEAISEGDGKYEIDADKCIDCGTC
                     ADTCPVGAPAQE"
BASE COUNT          332 a          241 c          297 g          367 t
ORIGIN      
        1 gtgtgttcgc agattttgga aaaaacgccc gataaaggat attgttgaag ttataggcgt
       61 catagcgggt actgcggcat attacgcatt tttaagtgag accgtaggct atcttttaca
      121 acacctgaat gcggagagct acaagggctc ggcttttttg tcggggattg cgctgttttt
      181 ggcggcgctt tttatgctgt tcggaaccct tgccgtaaag aatttctccg atgattcaat
      241 gaaaaagctt ataaactttg cgtttgcaat gatttgcacc gtaatactcg caaatgccgt
      301 tgttgcgata gttaaaatcc cgttcggccg tatgcgttac cgcgctatga ataccgccgg
      361 cggtgcttca ataggcggat ttgcgaattt cacaagatgg tatgtgagaa acggtcagat
      421 ggataaggcg caaatgatga cgctcttcgg cacgacagac gcgtgcaagt ctttcccgag
      481 cggtcacacc tgcgccgcgg gaatgagcta cgggcttata atgctcgcgg attctctcgg
      541 tataaagagt aagggcaaaa gagccgcact ttggatatgc ccgatattgt tcacgggaat
      601 tgttgccgta agcagaatag ttgtcggcgc tcacttcttt agcgacgtgc ttatgggcgg
      661 tactatttct tttctctcgg ttatgctttt ccgcgaaata ttcattttaa agggcgcaaa
      721 tctcaaagcc gtttttgcaa aaagcaaaga ctgataaaaa attttttaca ttatcaaata
      781 tggtattgaa attcaaaaaa tatatgttac aatgtgttta aacataataa aggaggtcat
      841 gtaaaatggc atataagatt tcggatgact gcatttcatg cggcgcttgt gctgctgagt
      901 gtccggttga ggctatcagc gaaggcgacg gcaagtatga aatagatgca gataagtgca
      961 ttgactgtgg tacttgtgca gatacatgcc cggtaggcgc tcccgcacag gaataattta
     1021 aagaaataaa ggtaagcgag gatattttcc ccgcttactt ttttatgccc cgaaaatgcg
     1081 gcgaagcaca catttttgct cgtttcggct tcggcggtat cgtcgggaaa atatattccg
     1141 ccgtttttcg ataaaaacat tgacttttta tgattttcaa attatattta tctcatacca
     1201 attagggagt gcatactttt gtacttccca acaatat
//
LOCUS       DBMDVO010000520         1237 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52330_length_1237_cov_1.190141, whole genome shotgun sequence.
ACCESSION   DBMDVO010000520 DBMDVO010000000
VERSION     DBMDVO010000520.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52330_length_1237_cov_1.190141"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="ACZPE2_07100"
     CDS             <1..1056
                     /locus_tag="ACZPE2_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013300.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR1319650.1"
                     /translation="YLSPWDRHEKTYGSGAAYNTYYKNQLRELLTNYGDIFCVWLDGA
                     CGEGPNGKVQKYDWNGYYALVRELQPNAVICVSGPDVRWIGNEAGVCRSSEWSVVPAW
                     LSKNDYIAEKSQKVDDEKFRKKHNEMTLDLGSRKAIKGETAFIWYPAEVDVSIRPGWF
                     YHENEDAKVKSLKKLYDIYIKSVGGNAALLLNIPPDKRGKIAKTDELTLDSFGRLLKR
                     RFPKNLASDAKATSSSEIDNEHLAKNIIEDDDSLYWQAASDDEEPEIVVDFGKPVNFD
                     NLVLQENIATGQQIESFKVYYEKNGRWKKLCKGTVIGYKKICLLRRVKTARRIKIVIT
                     SYRVKATLLKAEAYLSE"
BASE COUNT          369 a          274 c          308 g          286 t
ORIGIN      
        1 tatctgtccc cgtgggacag acacgaaaaa acctacggca gcggagcggc atataatact
       61 tattacaaaa accagctccg tgagcttctt acaaactacg gcgacatctt ttgcgtgtgg
      121 cttgacggcg cgtgcggcga gggccctaac ggtaaggtcc agaaatacga ttggaacggt
      181 tactatgccc ttgtccgaga gcttcagccg aatgcggtta tctgcgtcag cggacccgat
      241 gttcgctgga taggcaacga agcgggcgtg tgccgctcat ccgagtggag cgttgtgccg
      301 gcatggcttt ccaaaaacga ctatatcgcc gaaaaatcgc aaaaggttga tgacgagaag
      361 ttccgtaaaa aacacaacga gatgactctt gacctcggtt cgcgcaaggc gataaagggc
      421 gaaaccgcct ttatatggta tcccgccgaa gtagacgtct caattcgtcc gggctggttt
      481 taccacgaaa acgaggacgc aaaggtcaaa tctttaaaga aactctacga tatttatata
      541 aagtcggtag gcggaaacgc cgcactgctt cttaacattc cgcccgataa acgcggtaag
      601 atagcaaaga cggatgagct gacgcttgac agcttcggca gacttttaaa acgccgcttt
      661 ccgaaaaatc ttgcttctga cgctaaggcg acctcgtcaa gcgaaattga caacgagcat
      721 ctcgccaaaa acataataga ggacgacgac tcgctttatt ggcaggccgc gtcggacgat
      781 gaagagccgg aaatcgtagt ggatttcgga aaacccgtaa acttcgataa tcttgtgctt
      841 caggaaaata ttgcaacggg tcagcagata gagtcgttta aggtttacta cgagaaaaac
      901 ggcagatgga aaaaactttg taaaggcacg gttataggtt acaagaaaat ttgtctttta
      961 aggcgtgtga aaaccgcacg cagaataaaa atcgtgataa cctcatacag agtaaaggct
     1021 acactgctga aagccgaggc gtatctttcc gaatagaata gtggcgtaag gcctcggagt
     1081 ttaattgaat aatcaagctt gtaaaacggc actgtctgtg ccgttttatt ttatgctttg
     1141 cgccttcgac agcgaaaaac agctttgcaa agcagctttt aaaaatgagc ccgcaaaatg
     1201 ctgtggattt taaagattat gatttgacat atccgat
//
LOCUS       DBMDVO010000521         1236 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52371_length_1236_cov_1.521586, whole genome shotgun sequence.
ACCESSION   DBMDVO010000521 DBMDVO010000000
VERSION     DBMDVO010000521.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52371_length_1236_cov_1.521586"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="ACZPE2_07105"
     CDS             complement(<1..1117)
                     /locus_tag="ACZPE2_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009982878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1319651.1"
                     /translation="MAETKKALVERKAKTMDGNNAAAHVSYAFTEVAGIYPITPSSPM
                     ADYVDQWSAQGLKNIFGTTVKVAEMQSEAGAAGTVHGSLAAGALTTTYTASQGLLLMI
                     PNMYKIAGELLPCVFHVSARCVASHALNIFGDHSDVYACRQTGFAMLAETNPQEVMDL
                     GPVAHLAAIEGRVPFINFFDGFRTSHEIQKINVWDYKDLKDMVDMDAVKAFRERALNP
                     EHPTMRGSHENGDIFFQHREACNKYYDAVPAIVEKYMDKINEKIGTDYKLFNYYGAPD
                     AERVIVAMGSVCDVAEEVIDYLTAKGEKVGLVKVRLYRPFSAAKFVEAIPATAKKIAV
                     LDRTKEPGSIGEPLFLDTIAALDEMGRTGLKVSGGRYG"
BASE COUNT          320 a          296 c          297 g          323 t
ORIGIN      
        1 gaccgtagcg gccgccggaa accttaagac ctgttctgcc catctcgtca agagctgcga
       61 ttgtgtcaag gaagagcggc tcgcctattg aaccgggctc cttagttctg tcaagaacag
      121 cgattttctt agcagtagcc ggaatagcct caacaaattt tgcagccgag aacggtctgt
      181 aaagacgaac cttgacaaga cctacttttt cgccctttgc ggtgaggtaa tctataactt
      241 cttccgcaac gtcgcaaaca gagcccatag ctacgataac tctctctgca tcgggagcgc
      301 cgtagtagtt aaagagcttg tagtctgtgc cgatcttctc gttgatcttg tccatatact
      361 tttcaacgat agcgggaaca gcgtcataat atttattgca agcctctctg tgctggaaga
      421 agatatcgcc gttttcgtgc gaaccgcgca ttgtcggatg ctcggggttg agagcacgct
      481 cacggaacgc cttaacagcg tccatatcta ccatatcctt gaggtctttg taatcccata
      541 cattgatttt ctgtatctcg tgagaggtac ggaaaccgtc gaagaagttg atgaacggaa
      601 ctctgccctc aatagctgca aggtgagcta ccggtcccaa gtccataacc tcctgcggat
      661 ttgtctcggc aagcattgca aagccggtct gacggcaagc ataaacgtcc gagtggtcac
      721 cgaagatgtt aagtgcatga gaagcaacgc agcgagccga tacgtggaat acgcacggaa
      781 gaagctcacc ggcaatctta tacatattcg ggatcataag aagaagtccc tgtgaagcgg
      841 tgtaagtcgt agtgagagcg cctgcagcga gagaaccgtg aacggttccg gcagcgcctg
      901 cctcggactg catctcggca accttaactg tggttccgaa aatgttttta agtccctgtg
      961 ccgaccactg gtcaacatag tcagccatag ggcttgacgg ggtaatcggg taaatacccg
     1021 ctacttcggt aaacgcataa gatacgtgag cagcggcatt attaccgtcc atagtcttag
     1081 ctttgcgctc gactaaggcc tttttggttt ctgccatttg atatttcctc cttgaatgga
     1141 tatttaacag aatggatgta ttgtacatac tctgtaattt taacactttt atgaaattat
     1201 gtaaacctca ttttaattga aaaaaagggt aaattc
//
LOCUS       DBMDVO010000522         1232 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52631_length_1232_cov_1.016799, whole genome shotgun sequence.
ACCESSION   DBMDVO010000522 DBMDVO010000000
VERSION     DBMDVO010000522.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52631_length_1232_cov_1.016799"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="ACZPE2_07110"
     CDS             <1..735
                     /locus_tag="ACZPE2_07110"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1319652.1"
                     /translation="RQIELLEDGEKVMQETRRWDDNKGYSYAMRSKEDAQDYKYFPEP
                     DLPPIEISDEYIENVKNTLPELPEAKKARYMSQFGLPEYDTGIITSDVNLVKLFEHTV
                     EICNSPKDAANWIMGELMKMLNDTGTLSENMSFNPDSLGKLINMIKAGKINRGTAKSV
                     FEKIFTEDVDPEKYVEENNLGLVTDLSAIRPVIEQVIADNEKSVSEYKAGKTQAMGYI
                     MGQAMRALKGKAPAQEVQKILKEILG"
     gene            735..>1232
                     /locus_tag="ACZPE2_07115"
     CDS             735..>1232
                     /locus_tag="ACZPE2_07115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1319653.1"
                     /translation="MNTKYLFLDIDGTLVGFDGVMPSSASEALKKAHENGHKLVICTG
                     RQLSQVYPWLLKEIPFDGLILSGGAMVINDGKVVAHHFISQSSLKKLTDYFENSNTAY
                     YLQTQNFLVSKKWCVDRTLSLFNKLGYTKEDLLTLFGETVIDDNAGSRSDIEKLIYYD
                     SGVDFS"
BASE COUNT          378 a          281 c          290 g          283 t
ORIGIN      
        1 agacaaatcg agcttcttga ggacggcgaa aaggtaatgc aggaaacgcg ccgttgggat
       61 gataacaagg gatatagcta tgcaatgcgt tccaaggagg acgctcagga ttataaatac
      121 ttccccgagc ccgaccttcc gccgattgaa atctcggacg aatatataga aaatgtcaaa
      181 aacactctgc ccgagcttcc tgaagcaaaa aaagctcgct atatgtcaca gttcggtctt
      241 cccgaatacg acaccggaat cataacctcg gacgtaaatc tcgtaaagct ctttgagcat
      301 accgttgaaa tctgcaattc gcctaaggat gcggcaaact ggataatggg cgagcttatg
      361 aaaatgctca atgacaccgg caccctgtca gaaaatatgt cgtttaatcc cgattcgctc
      421 ggcaaactca taaatatgat aaaggccggt aaaatcaacc gcggcacggc aaaatcggta
      481 ttcgaaaaaa tattcaccga ggatgtcgat cccgaaaaat atgttgaaga aaacaacctc
      541 ggtcttgtca ccgacctttc ggctattcgc cccgtaattg agcaggtaat agccgacaac
      601 gaaaagagcg taagcgagta taaagcaggc aaaacccagg caatggggta catcatgggt
      661 caggcaatgc gcgctctcaa gggcaaggca ccggctcaag aggttcaaaa aatactcaag
      721 gaaattttag gctgatgaat accaaatatc tttttcttga catagacggc acgctcgtag
      781 gttttgacgg cgttatgccc tcgtcggcgt cggaggcgct gaaaaaagct cacgaaaacg
      841 gtcacaaact cgtaatctgc accggcagac agctctcgca ggtttatccg tggcttttaa
      901 aggagattcc ctttgacggt ttgattttat cgggcggagc tatggttata aatgacggca
      961 aggtggtagc tcatcacttt ataagtcaaa gcagtctcaa aaagctgacc gactacttcg
     1021 agaacagcaa caccgcatat tatctgcaaa ctcagaattt tctcgtatcc aaaaaatggt
     1081 gcgtcgaccg cacgctttcg ctctttaata agcttggata caccaaggag gatttactga
     1141 cgctcttcgg ggaaacggta atcgatgata acgccggaag caggagcgat attgaaaagc
     1201 tgatatatta cgattcgggt gttgattttt cc
//
LOCUS       DBMDVO010000523         1231 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52675_length_1231_cov_1.169027, whole genome shotgun sequence.
ACCESSION   DBMDVO010000523 DBMDVO010000000
VERSION     DBMDVO010000523.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52675_length_1231_cov_1.169027"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    complement(155..364)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(412..918)
                     /locus_tag="ACZPE2_07120"
     CDS             complement(412..918)
                     /locus_tag="ACZPE2_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319654.1"
                     /translation="METKKKKAAVTVLGIFVTALFIWLVIVGCNLYKNRWHKINFNVN
                     SITVVRTEGDTPYDGIYNVTVKGTASAWFYDFNKYQFDLVPASSGGYYPTFYQKTDTL
                     VVTNKDKTPFEFSFTTKENMDEDCVDTVSRFIYGAENITVNGSPDTSHDIRLFLSEYR
                     DKITVVNK"
     gene            complement(998..>1231)
                     /locus_tag="ACZPE2_07125"
     CDS             complement(998..>1231)
                     /locus_tag="ACZPE2_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1319655.1"
                     /translation="SILSVLGTFPETPTFKAAGLSRYADEKKLSAENPCLGNMKLFVN
                     SDAHRLSDIPEPQNELELPECSAEALIDYIKNR"
BASE COUNT          337 a          281 c          239 g          374 t
ORIGIN      
        1 tcttgacgct cggcatgaaa atacttatgc tcgagccatg tcttgtaaat acggtcttca
       61 acatttttcg gatcgtactt ttttgcaagc tccttgctca ttatttatcc tcctgtaaaa
      121 cataaattac aaaataaaaa cgcctcatac aaaagtatga ggcgaaaatt accgcggtac
      181 cactcatttt gccgtaaaaa acggccgctc tgtgccttta acggcggcaa accgtctttg
      241 cttaatgtag tttcggcaaa gacgctcaaa agtgacgacg gaaaacgccg aataccgctt
      301 cgcaccgaac agcggctctc ttaaaatgcg catcgctttc cttaagcttt ttcaacgcgt
      361 ttgaactatt tataatattt tatcattccg tacccgttat gtcaaacaaa actatttgtt
      421 cacgacggtt attttatctc tgtattccga caagaacagc cttatgtcgt ggcttgtgtc
      481 gggagaaccg tttacggtaa tattttcggc tccgtaaatg aaacggctaa ccgtatccac
      541 gcagtcctca tccatatttt cttttgttgt aaatgaaaac tcgaacggag ttttatcctt
      601 attggttaca accagggtat cggtcttttg atagaaggtg ggataatatc cgcccgacga
      661 agccggcaca aggtcgaact gatatttatt gaaatcataa aaccatgcgc ttgccgttcc
      721 ctttacggtt acattgtata ttccgtcgta aggagtgtca ccctcggttc tgacgacggt
      781 tatgctgttg acattaaaat ttattttatg ccatctgttt ttatagaggt tgcagcccac
      841 gataacaagc catataaaca aagctgtaac aaagatgccg agtacggtaa cggctgcctt
      901 tttctttttt gtttccatta tatataagac catccttatt ttcgtgtaat actcaatatt
      961 ataatcaaat tcggcaaaaa agcaacattt aaattattta acgatttttt atatagtcta
     1021 tcagagcttc cgcgctgcac tccggaagct caagctcgtt ttgcggctcc gggatatccg
     1081 aaagacggtg cgcgtcggaa tttacaaaaa gcttcatatt gccaaggcac ggattttccg
     1141 ccgaaagctt tttttcgtct gcataccgcg acaagcccgc cgccttgaag gtcggagtct
     1201 ccggaaaggt gccgagcacc gataaaatgc t
//
LOCUS       DBMDVO010000524         1231 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52679_length_1231_cov_1.061062, whole genome shotgun sequence.
ACCESSION   DBMDVO010000524 DBMDVO010000000
VERSION     DBMDVO010000524.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52679_length_1231_cov_1.061062"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="ACZPE2_07130"
     CDS             complement(<1..468)
                     /locus_tag="ACZPE2_07130"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR1319656.1"
                     /translation="MRKLIRIIAVILLIVGIGFFAYPIALRVVFNRQANQAIDQLEQL
                     KTDEFQTDDSVTDECDLPYAQLRKAMFDYNERLYVSGQSGLIDQLSYEKPDFLLSDYG
                     IDSDILAYITIPSIDVKLPIYNGASKENMAKGAAYLANTSLPVGGDNTNCVIAA"
     gene            complement(452..>1231)
                     /locus_tag="ACZPE2_07135"
     CDS             complement(452..>1231)
                     /locus_tag="ACZPE2_07135"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1319657.1"
                     /translation="SNYLYEKNSSKAITEHSENVNKLSPEIIAEEKEAIMRYNKSLFE
                     NTVVLTDPFDPDAYPLTDGEYSELLAFDDEMAYIEIPAINVYLPIYHGTAEETLLIGV
                     GHLENTSLPAGGLTTHAVLSAHCGLPSARLFTDLHLLKQDSIFRIHVLDETLTYQVYD
                     IETVEPDDRSSLFIQEGEDLVTLITCTPYGKNTHRLLVHGRRIEEKDEALIEEPKIEK
                     KMTWEDYTKLAAIGITAVFVLLLLGSAIKDLIRRKKHEKAD"
BASE COUNT          326 a          292 c          255 g          358 t
ORIGIN      
        1 ggcggcaatt acacaattgg tattgtctcc tccgacagga agtgaggtgt tggcaagata
       61 tgccgctccc ttagccatat tttccttgct tgcgccgtta taaatcggaa gtttaacatc
      121 gattgaggga attgtaatgt aagcaagtat atcactgtca attccgtaat cggagagtaa
      181 aaaatccggt ttttcatacg acagctggtc gatcagtccg ctttgcccgc ttacatacag
      241 cctctcatta taatcgaaca tcgccttgcg cagttgggca tacggaaggt cgcattcatc
      301 tgtaaccgaa tcatccgtct gaaattcgtc tgttttaagc tgttctaatt ggtcaatcgc
      361 ttgattcgcc tgcctattaa agacaaccct cagggcaata ggataagcaa aaaagccaat
      421 gccgacaatc aacaagataa cagctattat tctaatcagc tttctcatgc ttcttcctcc
      481 gtatcaagtc tttaatcgca cttccaagca gcaacagaac aaacaccgcc gtaataccga
      541 tggccgcaag ctttgtatag tcctcccatg tcattttctt ttcgattttc ggttcttcga
      601 tcagagcttc atccttttcc tcaattcgtc tgccgtgtac cagcaaacga tgggtgttct
      661 taccataggg agtgcaggtg atcagagtca caagatcctc gccctcctgt ataaaaagag
      721 aggacctgtc gtcaggttcg actgtctcga tatcgtaaac ctgataggtt aacgtctcgt
      781 ctagaacgtg aatacgaaaa atactgtctt gttttagcaa atgcagatct gtgaaaagac
      841 gtgcagacgg caaaccgcag tgcgccgata aaacggcatg tgtagttaat ccccctgcag
      901 gcagcgatgt gttttccaaa tgccctacac cgatcaacaa tgtttcttcc gctgttccgt
      961 gataaattgg cagatacaca ttaatcgcgg gaatctcaat atatgccatt tcatcatcaa
     1021 aagccaaaag ctcactatac tcgccgtcgg ttagggggta agcgtccggg tcaaacgggt
     1081 cagtcagtac cacggtgttt tcaaataggc ttttgttata gcgcattata gcctcttttt
     1141 cctccgcgat aatttcagga cttaatttat tgacgttttc cgaatgctcg gtaatagctt
     1201 tgctgctgtt tttctcataa agatagttgg a
//
LOCUS       DBMDVO010000525         1230 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52751_length_1230_cov_0.844110, whole genome shotgun sequence.
ACCESSION   DBMDVO010000525 DBMDVO010000000
VERSION     DBMDVO010000525.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52751_length_1230_cov_0.844110"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="ACZPE2_07140"
     CDS             complement(<1..109)
                     /locus_tag="ACZPE2_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1319658.1"
                     /translation="MFKLNIQLFAHKKGVGSTKNGRDSESKRLGVKRADG"
     gene            complement(97..390)
                     /locus_tag="ACZPE2_07145"
     CDS             complement(97..390)
                     /locus_tag="ACZPE2_07145"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016233.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1319659.1"
                     /translation="MIYSFEIKGHSGYAASGEDIVCAAISSTVWMTVNTLENILHLDV
                     EYKTSDADVFLEVSDRDIGSAQVLLEGFRQFLCNLSAEYEKYLTVKEVQKCLN"
     gene            complement(423..731)
                     /gene="rplU"
                     /locus_tag="ACZPE2_07150"
     CDS             complement(423..731)
                     /gene="rplU"
                     /locus_tag="ACZPE2_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1319660.1"
                     /translation="MYAVIETGGKQYQVKAGDVVFVEKIDTDAETFTFDKVIALGGEG
                     DFKVGAPYVDGATVIAKVLKQTKGKKVTVYRYKSKKGYHKKKGHRQPFTKVEIQTINA
                     "
     misc_feature    complement(745..820)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(852..>1230)
                     /locus_tag="ACZPE2_07155"
     CDS             complement(852..>1230)
                     /locus_tag="ACZPE2_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013250.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR1319661.1"
                     /translation="YNLITNFGKVMDPLADKLIVFAVILCLVKQSVFPVWCAMIILAR
                     DFLVTGMRVIAVSEGISVAASIWGKVKTTVQMIAMVIAILFGQSEIAALYIAAQTAIY
                     LATAATVISGISYIIDYRKIFEK"
BASE COUNT          318 a          315 c          229 g          368 t
ORIGIN      
        1 gaccgtcagc tctcttgaca ccaagtcttt tagactcgga atctctgccg ttctttgtgc
       61 tgccgacacc ttttttgtgt gcaaaaagct gtatgtttaa tttaaacatt tctgtacctc
      121 cttcaccgtg agatattttt cgtattccgc cgaaagattg cataaaaact gcctgaagcc
      181 ttccaaaagg acctgtgcac tgcctatgtc tctgtccgaa acttccaaaa acacgtccgc
      241 atcgctcgtt ttgtattcga cgtcaagatg cagaatattt tcgagtgtat tcaccgtcat
      301 ccaaacggtt gatgatattg ccgcgcaaac gatgtcttct ccggaagcgg catatccact
      361 gtgccctttg atttcgaagg aataaatcat tccttccgac aaataaaacg tcgcctcagt
      421 cattatgcat tgattgtctg gatttcaacc ttagtgaaag gctgtctgtg accttttttc
      481 ttgtggtagc ccttcttgga cttataacgg taaaccgtta ccttcttgcc cttggtctgc
      541 ttaagtacct ttgcgattac ggttgcgccg tcaacataag gagctccaac cttgaaatca
      601 ccttcgccgc cgagtgcgat aaccttgtca aaagtaaagg tctctgcgtc tgtatcgatc
      661 ttttcaacga atacaacgtc accggcctta acctggtact gctttccgcc tgtctcgatt
      721 actgcgtaca ttctttgcac ctccctgtat attcagactc gctatcgcgg gcggccttaa
      781 aagcacttga aaaaagcccg gtctatgcgg ttaccataat ataataccac aaaatgtacg
      841 ttctgtcaac cttatttttc aaatattttt ctataatcaa ttatatagct gattccggat
      901 ataaccgtcg ccgccgttgc aagatagatt gccgtctgcg ccgctatata caatgctgca
      961 atctcacttt gtccgaataa aattgcgata accattgcaa tcatctgaac ggtcgtctta
     1021 accttgcccc atatgcttgc cgcaaccgag attccttcgg acacggcgat gaccctcatt
     1081 cccgtaacca gaaagtcacg tgcaagaatg atcattgcac accataccgg aaaaacggat
     1141 tgttttacaa ggcatagtat aacggcaaaa acgatcagct tatcagccaa cggatccatt
     1201 actttgccga aatttgtgat aagattatat
//
LOCUS       DBMDVO010000526         1229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52776_length_1229_cov_1.464539, whole genome shotgun sequence.
ACCESSION   DBMDVO010000526 DBMDVO010000000
VERSION     DBMDVO010000526.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52776_length_1229_cov_1.464539"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="ACZPE2_07160"
     CDS             <1..832
                     /locus_tag="ACZPE2_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012312.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319662.1"
                     /translation="DTFPYDFLLGAAIMIIGMLVILFFVKEPDSRLKLKADMAEAADE
                     KAKRKAEKEAKKAEKERMKAEKLNPGERRSLVFMLMGLFFLFCGTNAISTFFALFAAE
                     ILHKTTAQATIMTTAFAAASALAALPAGWLGKKIGRKKTILGGLFIFVLAFAAYLITH
                     ILGIDALSGALIWVALIVGGAANMFITVNTLPLVLEIGGIDKVGTYTGYYYTATFSAQ
                     IATPIIYGIVRMFTGTYLSLFYYCPIAFILSILCILVVKHGEAIPQEIIDKVKEENED
                     "
     gene            complement(891..>1229)
                     /locus_tag="ACZPE2_07165"
     CDS             complement(891..>1229)
                     /locus_tag="ACZPE2_07165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1319663.1"
                     /translation="GESSCVKGVYDIIDEMVYNLCDNAVKYNRPGGSVNITVENKTIT
                     VADTGIGIPLSSQPRVFERFYRVDKSHSKEIGGTGLGLSIVKHAAIYHGAKIDLESVE
                     NVGTKIKISF"
BASE COUNT          323 a          299 c          264 g          343 t
ORIGIN      
        1 tgataccttc ccctatgatt tcctgctcgg cgcggcaatt atgataatag gtatgcttgt
       61 catactcttc ttcgtaaaag agcccgacag ccgattaaag cttaaagccg atatggcaga
      121 ggctgccgac gaaaaggcaa aacgcaaagc cgaaaaagag gctaaaaagg cagaaaaaga
      181 aagaatgaag gcggagaaat taaaccccgg cgaaagacga agccttgtat ttatgcttat
      241 gggtctgttc ttcctcttct gcggaacaaa cgcaatatcc actttctttg ctctttttgc
      301 ggctgaaatt cttcataaga caacggcgca ggcaaccata atgaccaccg cttttgccgc
      361 cgcttccgct ctcgccgctc ttccggcagg ctggctcggc aagaaaatcg gcagaaaaaa
      421 gaccatactc ggaggtctgt ttatatttgt tctcgcgttt gcggcttacc ttatcacaca
      481 tatactcggc atcgacgctt tgagcggtgc tcttatttgg gtagctctta tagtaggcgg
      541 cgcggcaaat atgtttataa ccgttaacac gcttcccctt gtgcttgaaa tcggcggcat
      601 tgacaaagta ggcacataca ccggctacta ttacaccgct acattctcgg cacagatagc
      661 tacgcccata atctacggca tagttcgtat gtttacaggc acatatctgt cgctgttcta
      721 ttactgcccg atagcattta tactctcaat actttgcata cttgttgtaa agcacggtga
      781 ggctattcct caggaaatca tagataaggt caaggaagaa aacgaagact gattaaatcc
      841 gcaatattaa agcagctgta aaatctcggg ttttacagct gcttttttgt ctaaaagctg
      901 attttaattt ttgttccgac gttttcgacg ctttcaaggt cgatttttgc gccgtgatat
      961 attgcggcgt gcttgactat cgaaagcccc agccccgtgc cgcctatctc cttggaatgg
     1021 cttttatcca cacggtaaaa gcgctcaaat accctcggct gagaggatag ggggattcct
     1081 attcccgtat ctgcaaccgt aatcgtttta ttctctacgg taatattgac gcttccgccc
     1141 gggcgattgt atttgacagc attgtcacat agattataaa ccatctcgtc gattatatca
     1201 taaactcctt tgacgcacga cgattcgcc
//
LOCUS       DBMDVO010000527         1228 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52844_length_1228_cov_1.230701, whole genome shotgun sequence.
ACCESSION   DBMDVO010000527 DBMDVO010000000
VERSION     DBMDVO010000527.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52844_length_1228_cov_1.230701"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="ACZPE2_07170"
     CDS             <1..920
                     /locus_tag="ACZPE2_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319664.1"
                     /translation="GLNAFITAQGFAKTGMLTVIIGAAANIVLDPLFIFVFNMGVRGA
                     AVATVISQGVSCVWAVCFLLSKKTGLKIRLKNMRLSLKIIMPCIALGLATFIMQASES
                     VISVCFNSSLLKYGGDIAVGAMTILTSVMQFAMLPLQGIGQGAQPIMSYNYGARNPDR
                     VKKTYRLLLIVNMIYAVILWAAIMLFPKAFAGIFSSDSELVGFAAKALRIYLGGMFLM
                     GAQIACQMAFVSIGNAPCSIIVAVVRKFVLLIPLIYIMPHIISDKAIGVYTAEPIADV
                     LAVTFTLILFAFKFRSAMKKLEKPEAAEK"
     gene            complement(1034..>1228)
                     /locus_tag="ACZPE2_07175"
     CDS             complement(1034..>1228)
                     /locus_tag="ACZPE2_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1319665.1"
                     /translation="MMITHNIKSALKLGTRTIMLECGKSVLDLKGDERNNMTVQDLLG
                     LYRQKSGKELDNDRMLLTGE"
BASE COUNT          301 a          263 c          302 g          362 t
ORIGIN      
        1 tgggcttaaa cgcgtttata acggctcagg gttttgcaaa aacaggtatg ctcaccgtta
       61 taataggcgc ggcggcaaat atagtgcttg acccgctgtt tatatttgtt ttcaatatgg
      121 gagtgcgcgg agcagctgtt gccacggtaa tttctcaggg cgtatcgtgc gtttgggcgg
      181 tgtgcttttt actgtcgaaa aagaccggac ttaaaattcg gctcaaaaat atgcgcctgt
      241 cgcttaaaat cataatgccg tgtattgcct tgggacttgc gacatttatt atgcaggcga
      301 gcgagagcgt aatttccgtg tgcttcaatt cttctctttt aaaatacggc ggcgatatag
      361 cggtcggtgc catgacaata ctgacaagcg taatgcagtt tgcaatgctt cccttacagg
      421 gcatagggca gggtgcccag cctataatga gctacaatta cggcgcgcga aatccggacc
      481 gtgtaaaaaa gacctaccgt cttttgctta ttgtcaatat gatttacgcc gtgattttgt
      541 gggcggcgat aatgctgttc ccaaaggcgt ttgccggaat tttcagttcg gattcggagc
      601 ttgtcgggtt tgccgcaaag gcactcagaa tttacttagg agggatgttc cttatgggcg
      661 cgcagatagc ctgccagatg gcgtttgtgt caataggcaa tgctccgtgc tctataatag
      721 tagccgttgt gcgcaaattc gttttgctga taccgctcat ctatataatg ccgcatataa
      781 tatccgataa ggccattggt gtttataccg cagagccgat agccgatgtt ttggccgtaa
      841 cctttacgct catactgttt gcgttcaaat tcagaagcgc aatgaaaaag cttgaaaagc
      901 ccgaagcagc cgaaaaatga cattgaaaaa agacacgatg aaaatcgtgt ctttttgttt
      961 aacgcatttg ttttacgggg tcgttaaatc ggaattgtgc cgactgaaat atacgcctgg
     1021 ttactccgaa acatcattct ccggtcagca gcattctgtc attgtcaagc tcttttcccg
     1081 acttttgacg gtagagtccc aaaaggtcct gaacggtcat attgtttctt tcgtcaccct
     1141 ttaggtcgag aaccgatttg ccgcattcaa gcataattgt gcgtgtgccg agcttgagcg
     1201 cggattttat attatgcgta atcatcat
//
LOCUS       DBMDVO010000528         1228 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52846_length_1228_cov_1.194321, whole genome shotgun sequence.
ACCESSION   DBMDVO010000528 DBMDVO010000000
VERSION     DBMDVO010000528.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52846_length_1228_cov_1.194321"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="ACZPE2_07180"
     CDS             complement(<1..777)
                     /locus_tag="ACZPE2_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1319666.1"
                     /translation="MNTPNAPWLKFYGDIPKTLEYPQISMYEAVVNAGKTHSQVNAYE
                     FQGKGTTYAKFIEKIDRTAKAYKALGIKEGETVTICMPNAPQGVDSFYALNRIGAVPA
                     MIHPLSAAGEITFYLKDTDSKAILVLDMFYEKVEQAVKDLGRPVKIIVARIQDELKFP
                     LNILYPLTLKKKPPELPDSENVIRWNDFIAAGKNETLPETFPSADKTAAILFSGGTTG
                     TSKGILLSNLNMNALGMEVAAAAGFTMEGLRMLSVMPLFHG"
     gene            complement(812..>1228)
                     /locus_tag="ACZPE2_07185"
     CDS             complement(812..>1228)
                     /locus_tag="ACZPE2_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1319667.1"
                     /translation="SPYVVAGNTAEVLLKLLMLEIYKLNPSPFGNTERQNKSMQKIRE
                     YIDAHYGEEITVEKLAESAYLSPPYLSHSFKSFSGFSPKQYLTAVRLVNANKLLTTTN
                     LSINEICRKAGFSDINNFIRTFKSFYGTTPKKFRSI"
BASE COUNT          274 a          268 c          306 g          380 t
ORIGIN      
        1 gccgtggaac aggggcataa ccgagagcat acgaagaccc tccatggtaa agccggccgc
       61 cgcggcaacc tccataccga gcgcgttcat attgagattt gagaggagta tacccttgct
      121 tgttccggtt gtgccgccgc tgaaaagtat ggccgccgtt ttgtcggccg agggaaatgt
      181 ttcgggcagc gtttcgtttt tacctgccgc gataaagtcg ttccaacgaa tgacgttttc
      241 gctgtcgggc agctccggag gctttttctt cagtgtgagc ggatacaaaa tgttaagggg
      301 aaactttaat tcatcctgta ttctcgctac gattatttta acgggtctgc cgagatcttt
      361 taccgcctgc tcaaccttct catagaacat atcgagaacg agtattgcct tactgtcggt
      421 gtctttaagg taaaacgtga tttcgcctgc cgcagagagc ggatgtatca tagccggaac
      481 ggcgcctatg cggttgagcg catagaagct gtcaactcct tgcggcgcat tgggcataca
      541 aatggttacg gtttctcctt cttttattcc gagagctttg taagcctttg cggttctgtc
      601 gattttttct atgaattttg cataagtcgt tcccttaccc tgaaattcat aggcatttac
      661 ctgcgaatgg gtcttgcctg cgtttacaac ggcttcatac attgagattt gggggtattc
      721 gagagttttc ggaatatccc cgtagaattt cagccatggt gcattaggtg tattcatatt
      781 aaatcctttc cgtatttttt attatttggt gttatatgct gcggaacttt ttaggtgtgg
      841 ttccgtagaa gcttttgaat gtgcgtataa aattatttat atccgaaaag ccggccttgc
      901 ggcaaatttc gtttatgctg agattggtag tcgtcaaaag cttgtttgcg tttacaagac
      961 gtaccgccgt gagatactgc ttcggactga agcccgagaa gctcttaaac gagtgactca
     1021 gatacggtgg ggacaaataa gcgctttcgg cgagcttttc gactgtgatt tcttcgccgt
     1081 agtgcgcgtc tatatactca cgtattttct gcatcgactt gttttggcgc tctgtatttc
     1141 cgaacggtga cgggttaagc ttatatattt caagcataag cagctttaaa agcacttctg
     1201 cggtattgcc ggcaacaacg tagggact
//
LOCUS       DBMDVO010000529         1227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_52897_length_1227_cov_1.151865, whole genome shotgun sequence.
ACCESSION   DBMDVO010000529 DBMDVO010000000
VERSION     DBMDVO010000529.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52897_length_1227_cov_1.151865"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="ACZPE2_07190"
     CDS             complement(<1..538)
                     /locus_tag="ACZPE2_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1319668.1"
                     /translation="MTAVCNKIKSIVAFAVICVILSLPLDAFASGDSDFVYYIKNGKV
                     CVSGYRGVSDTILVPEKLKGKTVAAIDDYAFSSNGTVEFIGLPDSVTTVGKNAFSDCT
                     ALSYVSFGANLNEIGDNAFSGCYSLKKAELKKGVTYIGKGAFKGCSSLSFAAIPDGVK
                     FIGEKAFFGCSALEKVTLP"
     gene            1037..>1227
                     /locus_tag="ACZPE2_07195"
     CDS             1037..>1227
                     /locus_tag="ACZPE2_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319669.1"
                     /translation="MTKDEYLITDPPLKALTVFAMPMILGSFFQQVYNMADSIIVGQF
                     VGSSALAAVGACAALTNVF"
BASE COUNT          344 a          272 c          239 g          372 t
ORIGIN      
        1 cgggcaacgt aactttttct aacgcagagc aaccgaaaaa ggctttctca ccgataaatt
       61 tcactccgtc gggaattgcg gcgaaagaaa gcgacgaaca ccctttaaag gctcccttgc
      121 ctatatatgt aactcctttt ttaagctctg ctttttttaa ggaataacag cccgaaaagg
      181 cattgtcgcc tatctcgttt aaattcgcgc cgaagctgac atatgacaga gccgtgcagt
      241 cggaaaaagc gtttttgccg actgtggtga cagaatcggg taagccgata aattccacgg
      301 ttccgtttga cgaaaatgcg taatcgtcaa tcgccgccac cgttttgccc ttaagttttt
      361 cgggtaccaa aatcgtgtcg ctcactccgc ggtagccgct tacgcaaacc tttccgttct
      421 taatataata cacaaagtcg ctgtcacccg aggcaaaagc atcgagaggc agcgacaata
      481 taacgcaaat aaccgcaaat gcaactattg actttatttt attacaaaca gctgtcattt
      541 cattcctccg ccccttttat gagcttgatt gtatcacctt tttcggcttt tttcggaatt
      601 tgtgctgatt tatcacacaa actcatttcc gccgctttat attcttttta caaaacatcg
      661 cattcaaggt aacaaacggc gtcataaaat aatatgcggc taaatgccga ctgaaacagc
      721 acaccggata ttggatgtgt gctgttttct gttctgattt atatttaaaa cgacactttt
      781 tactgcttat taaatatcaa cggtcaggct attcaggact ataactcatt gacatttatt
      841 tatgccgatg atatacttct aatgttcgct cagggagcga ttgttcagta aagacgtaag
      901 ccgaacaggg tgcagactga gaaagctgta tcttgtttgg ctttttaaat ataacattgc
      961 ttttgtgcta caattctaag aaaacggata cataaatcaa aaggataacg caagtgattt
     1021 tcaggagaat attaaaatga caaaggacga atatttaata accgatccgc cgctcaaggc
     1081 tttgactgtt tttgcgatgc ctatgatttt aggcagcttc tttcaacaag tatacaatat
     1141 ggctgattca attatcgtcg gtcaatttgt cggctcctct gcccttgcgg ccgtcggggc
     1201 ttgtgccgct cttacaaatg tttttat
//
LOCUS       DBMDVO010000530         1225 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53009_length_1225_cov_1.253559, whole genome shotgun sequence.
ACCESSION   DBMDVO010000530 DBMDVO010000000
VERSION     DBMDVO010000530.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53009_length_1225_cov_1.253559"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..626)
                     /locus_tag="ACZPE2_07200"
     CDS             complement(156..626)
                     /locus_tag="ACZPE2_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1319670.1"
                     /translation="MNIRRAEEKDIPRIIELLKQVLQIHADIRPDIFISGTTKYTTDE
                     LVKMINDDKKPIYVAVNGSGICVGYAFCQLQEQSVSNNTVPFKSLYIDDLCVDKEQRG
                     KHIGEALFDYVKKEAKRMDCYEVTLNVWTGNTPAEKFYEKMGLKTKKRMMEYIL"
     gene            complement(693..>1225)
                     /locus_tag="ACZPE2_07205"
     CDS             complement(693..>1225)
                     /locus_tag="ACZPE2_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015338.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR1319671.1"
                     /translation="KFSAVEKNTIWLSETPEKESKYTYTDKDGNHCEAGCYRICSYVV
                     LLNKQNGKNIIFLNTHLDNASEQAADFGANVVMNKLNELKEKYNNTDCTVLTGDFNET
                     QDGTAYKLVASKLNDGTNRAKKTATYQEWCYRSTGNEPIDFIFTDGKAVDYTVLNDLN
                     NGYVSDHYGVYSGINF"
BASE COUNT          292 a          273 c          227 g          433 t
ORIGIN      
        1 aaagtctact actttatttt tctattttgc aatctctatt gaaaaagtac cattgttatt
       61 aaacgccggc ttaaagcaaa gcagctccac agcctttgcg tatactccgt atttcaacac
      121 tttattcacc tgcgattgag ataattttcg ttcggttaca gtatgtattc catcattctt
      181 ttctttgttt tcagccccat tttttcatag aatttttctg ccggagtgtt ccctgtccat
      241 acattcagtg ttacttcgta gcagtccatc ctttttgcct cttttttgac gtaatcaaac
      301 agagcctcac cgatatgctt tcctctttgt tccttgtcta cgcacaaatc gtcgatatac
      361 agcgacttaa acggtactgt attgttagaa acagactgtt cctgtaactg acaaaaggca
      421 tagcccacac agatgccact gccgtttacg gcaacataaa tcggcttttt gtcatcattt
      481 atcattttca ccaactcgtc agtcgtgtat ttggtggtgc cggatataaa aatatccggg
      541 cgtatgtcgg cgtgtatttg gagcacctgc ttcaataact cgataatcct cggtatatcc
      601 ttttcttccg ctcttctgat attcatactg tattagctcc taatgaataa attttcgaat
      661 tcattatata aagattattc ttgtcagtca atttaaaagt taatacctga atatactccg
      721 tagtggtcgg acacataccc gttgttcagg tcgttcagca cggtataatc tactgctttt
      781 ccatcggtaa atataaaatc gataggctca ttaccggtag agcgatagca ccactcttga
      841 tatgtggctg tcttttttgc tctgttcgtg ccgtcgttca gcttggacgc aacgagctta
      901 taagccgttc cgtcttgggt ttcgttgaaa tcaccggtca aaacggtgca gtcggtattg
      961 ttgtattttt cttttagctc attgagtttg ttcataacta catttgcacc gaaatcagct
     1021 gcctgctccg atgcgttatc cagatgagtg ttcaaaaaaa tgatgttttt gccgttctgc
     1081 ttattcagca gaacaacata gctgcaaatt ctgtagcatc cggcttcaca gtgattaccg
     1141 tctttgtcgg tgtaggtgta tttgctttcc ttttcgggag tttcgctgag ccagattgtg
     1201 ttcttttcca cagcggaaaa cttgg
//
LOCUS       DBMDVO010000531         1223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53120_length_1223_cov_1.147059, whole genome shotgun sequence.
ACCESSION   DBMDVO010000531 DBMDVO010000000
VERSION     DBMDVO010000531.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53120_length_1223_cov_1.147059"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="ACZPE2_07210"
     CDS             complement(<1..1083)
                     /locus_tag="ACZPE2_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR1319672.1"
                     /translation="MKKQKIPNKKVIALAGNPNVGKSTVFNALTGMHQHTGNWTGKTV
                     DSAEGRLLYKEREYVLVDLPGTYSLCSHSEEEENAGKFLQSQRYDAVIVVCDAVCLER
                     NLNLVLQAMEITGNIIVCINLLDEAKKKGIEIDTEKLEKRLGVPTVGICARTKQGFPE
                     LLAAVQKMSADKKAPIFPVKYSESTEKAIEILCDALRKNTNLPLNERYMALRLLTEPK
                     KNAEEFKEIFGDDKLKGCDISDALERAYRVCGGRENIEDDIPESIVKTAENAVDGVVK
                     TNQNKKHDFDRKLDKLFTSKLTGIPIMLLLLLLIFWITAVGANYPSELLQRASGFLTQ
                     KLMLLLTNAGVSVWLREMLVNGMFKVL"
BASE COUNT          286 a          293 c          244 g          400 t
ORIGIN      
        1 taagacctta aacattccgt ttacaagcat ttcgcgcagc cacacagaaa cgccggcatt
       61 tgtcaaaagg agcataagct tttgtgtgag aaaaccgctt gcacgctgta aaagctcgct
      121 cgggtaattg gcacccaccg cggtaatcca aaatatgagc agtaaaagca gcaacattat
      181 cggtattccc gtaagcttac ttgtgaaaag cttatctaat ttgcggtcaa agtcatgctt
      241 cttgttttga tttgttttta caacgccgtc aactgcattt tcggctgttt taactatgct
      301 ttcgggaata tcgtcttcta tgttttctct tccgccgcac acacggtacg ctctttccag
      361 cgcgtccgaa atatcgcagc cttttaattt gtcgtcgccg aatatctcct taaattcttc
      421 cgcgtttttt ttcggttctg ttaaaagacg cagagccatg tatctttcat tcagcggaag
      481 atttgtattt tttcttaatg cgtcgcagag aatttctatt gccttttcgg tactttcact
      541 gtattttacg gggaaaatcg gtgctttttt atctgccgac attttttgaa ccgcggccaa
      601 aagctccgga aacccctgct ttgtacgtgc acaaatgccg actgtcggaa caccgagtcg
      661 tttttcgagc ttttccgtgt caatttctat gccctttttc ttcgcctcat ctaacaggtt
      721 tatacaaaca attatattac ccgttatttc catggcttgc aggacgagat ttaaattgcg
      781 ctcaaggcag accgcgtcgc atacaacgat tacggcgtca taccgttgag attgcaaaaa
      841 tttacctgca ttttcctctt cttcggagtg tgagcaaaga gaatatgtac ccggcagatc
      901 gaccaaaacg tattctcttt ctttataaag aagcctgccc tctgcggagt ctaccgtctt
      961 gcccgtccaa ttaccggtgt gctggtgcat acctgtgagc gcgttaaata ccgtgctttt
     1021 tccgacgttc ggattgccgg caagtgctat tactttttta ttgggaattt tttgtttttt
     1081 caaattatca ccctatttaa gaagtatctg ctctgcgtcg gactttcgta tcgcaatgta
     1141 tgcaccgcgt atcatgtaga cttttatgtc gccggatatg ctgtcgagaa cacaaccgat
     1201 ttcggtgtcc cttataactc cga
//
LOCUS       DBMDVO010000532         1223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53124_length_1223_cov_1.094474, whole genome shotgun sequence.
ACCESSION   DBMDVO010000532 DBMDVO010000000
VERSION     DBMDVO010000532.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53124_length_1223_cov_1.094474"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(590..1207)
                     /locus_tag="ACZPE2_07215"
     CDS             complement(590..1207)
                     /locus_tag="ACZPE2_07215"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIR1319673.1"
                     /translation="MFSYEKMLQYPVNIKNPNPKYAQIVLEQYGGPDGELGASLRYLS
                     QRFSMPYPELKGLLTDIGTEELGHLEMIGAIVYQLTRNLTQEQIKQGGFDAYFVNHTT
                     GVYPAGANGVPFNAGAIASKGDVITNLHENMAAEQKARTTYDNILRLVDDPDVRDVIK
                     FLREREVVHYQRFGEGLRIATDKMNEKNFYAFNPSFDTVCNKKTR"
BASE COUNT          327 a          290 c          229 g          377 t
ORIGIN      
        1 ttcgaaagat tcttctttct tcggctgtct tagcagactc aaacaggcga attccccata
       61 atgccttacc atgacgtttg ataatattgt acgctgaatc cgtcactgcc catcctacag
      121 aaccggttcc catatccaat ccaatatagt actcttcaaa ttttttcata aaatcctcct
      181 ttttctcttg acatgtcgta gtttatgctt tattattgat gtcagttaag taccatacgt
      241 attaactaca tcataagttc aaataaaaat ttattctaac cgttgctttg gcaacatcac
      301 agtgtgtgag ttgagacctg cttttgcagg tctttttatt tttcccggat taagcctgaa
      361 atgtcatcac cacagtgccg cgcaaattaa aaactgcact attgtcattt tatcataaaa
      421 aataacgggt gtaaaccgca cattgtgttc gaacgcaaca aatgtccgta tttatgtaca
      481 tattaaagac cgtccctgat taggaacggt ctttgcttta tattttttac gaacgtgacc
      541 gcgccgtttg cagaaaaacc gctccaaggc agagcgttaa caagcaaaat cagcgcgttt
      601 ttttgttaca taccgtatca aagctcggat taaaggcgta gaaatttttc tcgttcattt
      661 tgtccgtcgc aattctgaga ccctcgccga aacgctgata atgaaccacc tcgcgctcgc
      721 gcaggaattt tataacatct ctcacatccg gatcgtcaac aagtctcaaa atattgtcat
      781 aggtagttct tgccttttgc tcggccgcca tattctcatg caaatttgtt atgacgtctc
      841 cctttgaagc tatggcaccc gcattgaacg gcacgccgtt cgcccctgcc gggtacaccc
      901 ccgttgtatg atttacaaag tatgcgtcaa agccgccttg cttaatttgc tcttgagtaa
      961 gattacgagt aagctgataa actatggcac ctatcatttc gaggtgaccg agctcctcgg
     1021 taccgatgtc ggtgagaagt cctttaagct caggatacgg catcgaaaaa cgctgagaga
     1081 ggtagcgaag cgaagcaccg agctcaccgt cgggtccgcc gtactgctct aatactatct
     1141 gagcgtattt gggatttgga ttttttatat ttaccggata ttgcagcatt ttttcatacg
     1201 aaaacattat ttaacacact cct
//
LOCUS       DBMDVO010000533         1221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53225_length_1221_cov_1.131250, whole genome shotgun sequence.
ACCESSION   DBMDVO010000533 DBMDVO010000000
VERSION     DBMDVO010000533.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53225_length_1221_cov_1.131250"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="mreB"
                     /locus_tag="ACZPE2_07220"
     CDS             <1..965
                     /gene="mreB"
                     /locus_tag="ACZPE2_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1319674.1"
                     /translation="FVKGKGIVIREPSVVAVDVSAYPQKVVAVGNKAKQMIGRTPGSI
                     TAVRPLKDGVIADFEITSDMLKEFIKMAISSSPFSKARVLICIPSGVTEVERKAVHDA
                     ARSAGAKYVSLIEEPMAAAIGAGLPVSEAIGNMVVDIGGGTSEVAVISFGDIVTAQSA
                     RVAGDNFDESIIAYIRKKHNLLIGERTAEDIKIKIGSAYPYDGEGAMDVKGRNLLDGL
                     PKNIEITSEEVREALSDPVNQILDAVRSTLEKTPPELSADIIDRGIMLTGGGALLRGL
                     DKLISIETKIPVHVAENPLDCVVDGCGVCLESNTLGITAKKNYD"
     gene            1025..>1221
                     /locus_tag="ACZPE2_07225"
     CDS             1025..>1221
                     /locus_tag="ACZPE2_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319675.1"
                     /translation="MKKFFKSTAFKILLAIALVLLAAIAAAASAADGTTPVSKVTSFV
                     FSPLQKASSYLVDKLDGFKGS"
BASE COUNT          314 a          310 c          311 g          286 t
ORIGIN      
        1 ttttcgttaa aggtaaggga atagtaatcc gtgaaccatc cgttgttgcg gtcgatgtca
       61 gcgcttatcc gcaaaaggtt gtcgcagtcg gcaacaaggc taagcaaatg ataggccgta
      121 cacccggctc aataaccgcg gtcagacctc ttaaagacgg tgttatcgcc gactttgaga
      181 taacctcgga tatgcttaag gaattcataa aaatggccat aagctcatcg ccgttttcaa
      241 aggcgagagt tcttatctgt atcccgtccg gagttacaga ggtagagaga aaggccgttc
      301 atgacgcggc acgctcggca ggcgcaaaat atgtaagtct tatagaagag cccatggcag
      361 ccgcaatagg cgcgggtctt cccgtttcgg aggccatagg caatatggta gtcgatatag
      421 gcggcggtac aagcgaggtt gccgtcattt ccttcggcga catagttacc gctcagtccg
      481 caagggttgc cggcgataat ttcgacgagt ctattatcgc ttacatcaga aagaagcaca
      541 acctccttat aggcgagcgc accgcagagg atataaaaat aaaaatcggc tccgcctacc
      601 cctacgacgg cgagggcgcc atggacgtca agggcagaaa cctgcttgac ggcctcccca
      661 aaaacatcga aattacttcc gaagaagtcc gcgaggctct ttcggacccc gtaaatcaga
      721 tcctcgacgc agttcgctcc actcttgaga aaactccccc ggagctttcg gcggatatta
      781 tcgaccgcgg cattatgctc acaggcggcg gcgcattatt aagaggactt gacaagctca
      841 tttccattga aacaaaaatc cccgtccacg ttgcggaaaa tccgcttgac tgcgttgttg
      901 acggctgcgg cgtctgcctt gagagcaaca ctctgggcat taccgccaaa aagaattacg
      961 actgataaaa aggcgggcgg aattgttttt cgcccgcaaa ttttaactgt gaccggagta
     1021 cgatatgaag aaatttttta agagtacggc ttttaaaata ctgcttgcga tcgcgcttgt
     1081 attgcttgct gccattgctg ccgcggcttc tgccgcagac ggcacaacac ccgtttcaaa
     1141 ggtgacgtcg tttgtatttt ctccgctcca gaaggcctcg tcgtatcttg tagacaagct
     1201 cgacggcttt aagggaagct t
//
LOCUS       DBMDVO010000534         1221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53240_length_1221_cov_0.908929, whole genome shotgun sequence.
ACCESSION   DBMDVO010000534 DBMDVO010000000
VERSION     DBMDVO010000534.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53240_length_1221_cov_0.908929"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="ACZPE2_07230"
     CDS             complement(<1..1185)
                     /locus_tag="ACZPE2_07230"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR1319676.1"
                     /translation="MRKLELPVGNDVVVSFTHDFSLENEEYRLEVNEKISVYSSGEAG
                     KVFALQTLKQLLFEYGRTIPFIAIKDKPKYKIRGFMLDVGRYFYPVDDVKLFIRKMSL
                     HKLNFLHLHLTEDQGWRVEIYKYPLLTQIGSVRKKTNFNHRQHKGYYTKAQIKSIVKY
                     AHDFGISVMPEFDIPGHSRSALACYNYLGCFERNLPVADHWGVKHDVLCAGKESTYKF
                     VEDIVDELCELFPDKYFHIGGDEVPKHRWHLCPHCQAMMKKLGLNNEDELQCCFMNRI
                     NDYCAGKNKQAFMWSWDLKDDKLLSENLGFTKCGDINTGGRPFIDTSSKAYYIDLPYG
                     YISLKDTANHKLYDGNCLGAEATLWTEYVPNMKKADKMTYPRLGAMCETVWRGENSYE
                     QFH"
BASE COUNT          350 a          300 c          197 g          374 t
ORIGIN      
        1 atgaaactgc tcatatgaat tttctccgcg ccaaacagtt tcacacatag ctccaagcct
       61 aggataagtc attttgtccg cttttttcat attgggaaca tattccgtcc ataacgttgc
      121 ttccgcaccg aggcaatttc cgtcgtataa tttatggttg gcggtatctt tgagacttat
      181 gtagccgtaa ggcaaatcta tataatatgc ttttgaactt gtatcgataa acggtctgcc
      241 gcccgtgttt atgtccccgc atttcgtaaa gcccaaattt tcggaaagca gcttatcatc
      301 cttcaaatcc caactccaca taaacgcctg cttattcttg cctgcgcaat aatcattaat
      361 tctgttcata aaacagcact gaagctcatc ctcgttattt aagccaagct tcttcatcat
      421 tgcctgacaa tgcgggcaaa gatgccatct gtgtttcggc acctcgtcgc cgcctatatg
      481 aaaatactta tccggaaaaa gctcacaaag ttcatccact atatcctcga caaatttata
      541 tgtgctttct ttccctgcac acaaaacatc atgctttact ccccaatggt ctgcgaccgg
      601 caaatttctt tcaaaacagc cgaggtaatt ataacaagcc agcgcacttc tgctgtgccc
      661 cggaatatca aattcaggca taaccgaaat accgaaatca tgtgcatatt tgactatact
      721 tttaatctga gcttttgtgt aatagccctt atgttgccta tgattaaaat ttgtcttttt
      781 ccttacagac ccgatttgcg tcagcaacgg atatttgtaa atctcaactc gccatccctg
      841 gtcctccgtc aaatgcaaat gcagaaaatt aagcttgtgc agagacattt ttctaataaa
      901 cagcttgaca tcgtcaacgg gatagaaata tctcccgaca tcaagcataa agcctctgat
      961 tttatactta ggcttatcct ttatcgcaat aaacggaatc gttcggccgt attcaaacag
     1021 tagttgcttg agtgtttgca gagcaaacac ttttcctgcc tccccactcg aatacacgga
     1081 gattttctca tttacttcaa gtctgtattc ttcattttca agcgaaaaat catgcgtaaa
     1141 actaacaaca acatcatttc cgaccggcag ttcgagttta cgtataaaat cataaaaatc
     1201 atctttaaca gaaccgttga c
//
LOCUS       DBMDVO010000535         1220 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53314_length_1220_cov_0.759607, whole genome shotgun sequence.
ACCESSION   DBMDVO010000535 DBMDVO010000000
VERSION     DBMDVO010000535.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53314_length_1220_cov_0.759607"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="ACZPE2_07235"
     CDS             complement(<1..227)
                     /locus_tag="ACZPE2_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR1319677.1"
                     /translation="MICQLVKRNLKLHVACLNHKRKKELREFGATTRELMELAEWLQK
                     NECEIVAMESTGSYWKPLYNIMESYGLHAIVV"
     gene            309..551
                     /locus_tag="ACZPE2_07240"
                     /pseudo
     CDS             309..551
                     /locus_tag="ACZPE2_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
     gene            581..>1220
                     /gene="mreB"
                     /locus_tag="ACZPE2_07245"
     CDS             581..>1220
                     /gene="mreB"
                     /locus_tag="ACZPE2_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1319678.1"
                     /translation="MFKMFGMGETIGIDLGTANVLVYVKGQGIVLNEPSVVAVDRDTN
                     TILAVGEEARRMLGRTPGNIAAIRPLKQGVISDYELTEKMIKYFIDKAFIRKTLVKPT
                     VTVCIPSGVTEVEKRAVEDAVHQAGARHVVLIEEPVAAAIGAGIDISKACGSMIVDIG
                     GGTTDIAVLSLGGTVVSQSIKIAGDDFDEAIIRFIRKKHNVLIGERTAEQLKI"
BASE COUNT          373 a          239 c          311 g          297 t
ORIGIN      
        1 actacaatcg catgaagacc ataggattcc atgatgttat acaatggttt ccagtaagaa
       61 ccggtgcttt ccatcgcaac gatctcacat tcattttttt gcagccattc tgccagttcc
      121 atcagttccc gggtggtcgc accgaattcc cggagttctt tcttgcgttt atggttcaga
      181 caggcaacat gaagcttaag gttccgctta acgagctgac atattatgag cctaaaaaag
      241 agcctaagtc gaatgcttca agaaacattt ccacaaaagt gcgtgccgga aagagccagt
      301 atattgcgtc agaaatagac tgccgtggcc agaatgtaga ggaagggctc ggaaacatag
      361 acaagtatct tgatgacgca ttccttgccg gacttaagac ggttacaata atccacggta
      421 aaggaacagg cgtgcttcgt gccgctgtac agaaatatct taggacaaat cctcatgtaa
      481 gaaccttccg tcccggagta tacggagaag gcgaaatggg cgttactatt gtggagctta
      541 aaaattcgta ataaaacaaa acataataag gagaataatt atgtttaaaa tgtttggaat
      601 gggtgaaacc atcggcattg acctcggaac ggcgaatgtg cttgtgtatg tcaagggaca
      661 gggaattgtg ctcaatgagc cgtctgtggt tgccgttgac agagatacaa acacaatact
      721 tgccgtaggc gaagaggcaa gacgtatgct tggaagaacg ccgggaaaca ttgcggcaat
      781 aagaccgctt aaacagggcg ttatttccga ttatgagctt acggaaaaga tgattaaata
      841 ttttatagat aaggcgttta taagaaaaac gcttgttaag ccgaccgtta cggtatgtat
      901 accgagcggc gttacagagg tggaaaagcg tgccgttgag gacgctgtcc atcaggcagg
      961 agcaagacat gttgtgctta tagaagagcc tgttgcggcg gctatcggtg caggaataga
     1021 catatcaaag gcctgcggaa gcatgatagt cgatattggc ggaggaacaa cggatatagc
     1081 tgtgctttct ctcggcggta cggttgtcag ccagtcgata aaaattgccg gtgacgactt
     1141 tgacgaggct attatacgct ttatcagaaa aaaacataat gtgcttatag gcgaaagaac
     1201 agccgaacag cttaaaataa
//
LOCUS       DBMDVO010000536         1219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53363_length_1219_cov_0.929338, whole genome shotgun sequence.
ACCESSION   DBMDVO010000536 DBMDVO010000000
VERSION     DBMDVO010000536.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53363_length_1219_cov_0.929338"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            354..980
                     /locus_tag="ACZPE2_07250"
     CDS             354..980
                     /locus_tag="ACZPE2_07250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1319679.1"
                     /translation="MSAENSPGKCEYTIVIDAGHGGADGGAVASDGISEKVINLEIAY
                     KLNYILRAYGLNTVMTRTDDESIHDSNAKTLREQKVSDIHKRMSIMESDENNIFVSIH
                     QNKFSNSSLWGTQVFYSPNTTDSAVLANCIQNSVCSLLQNDNKRVIKKSGSNIYLLYY
                     AKRTAVLVECGFVSNPQENQLLENSDYQRKMAFSIAFGIMEYINTKDV"
     gene            983..>1219
                     /locus_tag="ACZPE2_07255"
     CDS             983..>1219
                     /locus_tag="ACZPE2_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1319680.1"
                     /translation="MASKLKTVFVCSKCGYESAKWFGQCPGCHEWDTMNEEVKAPQTV
                     TAKRTYSDNFHGKVYKLNDIVTDTEHRYDTGLHEL"
BASE COUNT          410 a          193 c          260 g          356 t
ORIGIN      
        1 ccaatatggt taagggctaa tattttctga tattaaagat aaagagcaag ccaagaggct
       61 tgctcttttt tttgttgaaa acaaaagtgt gttatgatac aatttataat gatgatttta
      121 gcgtgtaaga acacctttgc atgatgaaca aaacaatcgc atatatagga cgttttaatt
      181 gaaaacggca aaaaatatga tatactgata gatataataa tcagcacgaa aagctcataa
      241 aatttaacgg gtgatgtttt atgagcaaaa gggtgtttcc catatgtata tttattttat
      301 gctttttttg tgtaggcttt tatgtttttg aatttctacg tgccgcaagt aatgtgtcgg
      361 ctgaaaattc accgggaaaa tgtgaataca caatcgtgat tgacgccggg cacggcggtg
      421 ccgacggcgg agcagttgcg agtgacggca tatcggaaaa ggtaatcaat ttggaaatcg
      481 catataagct gaattatatc ctcagagcat acgggcttaa taccgttatg acaagaacag
      541 acgacgagtc gatacacgac agtaacgcaa aaactctgcg tgagcagaaa gtttccgata
      601 tacataagcg tatgtcgata atggaatcag acgaaaacaa tatcttcgtc agtatccatc
      661 aaaataaatt ttccaacagt tcgctttggg gtactcaggt tttttattcg cctaacacta
      721 ccgatagtgc agttttggct aattgcatac aaaattctgt ttgcagtctt ttgcaaaatg
      781 ataataagcg tgttattaag aaatcaggaa gtaatattta tttgctgtat tacgcaaaaa
      841 gaactgctgt gcttgttgag tgcggatttg tttcaaatcc tcaagaaaac caattgcttg
      901 aaaactcgga ttatcaaaga aaaatggctt tttccattgc ttttggaata atggaatata
      961 taaatacaaa ggatgtgtaa tagtggcatc aaagttaaaa acggtttttg tgtgttcaaa
     1021 atgcgggtat gagtctgcaa aatggttcgg acagtgccct ggatgccatg agtgggacac
     1081 aatgaacgag gaagtgaaag ctccgcaaac cgttacggca aaaagaacct attcggacaa
     1141 tttccatgga aaagtatata aattaaacga tattgttact gatactgagc atagatacga
     1201 cacgggactg cacgaactt
//
LOCUS       DBMDVO010000537         1215 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53596_length_1215_cov_0.929982, whole genome shotgun sequence.
ACCESSION   DBMDVO010000537 DBMDVO010000000
VERSION     DBMDVO010000537.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53596_length_1215_cov_0.929982"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="ACZPE2_07260"
     CDS             complement(<1..751)
                     /locus_tag="ACZPE2_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319681.1"
                     /translation="MKEKKKIGSKIFMALMMIFFYAPIVYIIIFSLNSSRSLTHLDGF
                     SLRWYEKMFSDRTMMQSIVYTIVIAVLATIISTIVGTITSIGLSKSKPILKKTVEQIN
                     NLPIMNPEIVTAIGLLMFFSALGAKKGFATLLLAHIMFCIPYVMLSVMPKLRSLDANL
                     ADAAMDLGATPFQALTKVIVPQIMPGIVSGALIAFTMSFDDFIISYFVTGNGVSNISI
                     LVYTMSKRVNPSINALSTLVIFIITVALIIVN"
     gene            complement(754..>1215)
                     /locus_tag="ACZPE2_07265"
     CDS             complement(754..>1215)
                     /locus_tag="ACZPE2_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319682.1"
                     /translation="NTILGLFGIGTVKIIYTNFAVILGMVYNFVPFMILQIYSSLEKM
                     DKSYIEAASDLGANKYQCFWRITFPMSLPGVISGITLVFLPAVSSFFIPKLLGGGQYV
                     LIGNVIETQFLTAGNWSFGSAISLIMAIIIMISLYITRKLDTDPAAAKGGA"
BASE COUNT          369 a          250 c          230 g          366 t
ORIGIN      
        1 catttacaat aataagagcc actgtaataa taaatattac cagtgttgaa agtgcgttaa
       61 tcgaaggatt aacacgttta ctcattgtat acacaagtat tgatatgttg cttactccgt
      121 ttcccgttac gaaatatgaa attatgaaat cgtcaaagga catagtaaat gctatcagtg
      181 ctccggaaac tataccgggc attatctgcg gaacaataac ctttgtcaat gcctggaaag
      241 gcgttgctcc caaatccatt gccgcatcgg caaggtttgc atccagtgac ctgagcttcg
      301 gcattaccga aagcattaca tacggtatgc agaacattat atgtgccaaa agcagtgtag
      361 caaagccttt ttttgcaccc aaagcactga aaaacataag aaggcctatt gccgttacga
      421 tttccggatt cataatagga aggttattta tctgttcaac cgtcttttta agtatcggct
      481 ttgactttga cagacctatt gatgtaatcg ttccgactat tgttgatatt atggttgcaa
      541 gaaccgcaat aactatcgta taaacaatcg actgcatcat tgtcctgtcg ctaaacatct
      601 tttcgtacca tctaagtgaa aatccgtcaa gatgtgtgag tgaacgggag ctgttgagcg
      661 aaaatataat tatatacaca ataggtgcat agaaaaatat catcattaag gccatgaata
      721 tttttgagcc tatctttttc ttttccttca taattatgct cctcccttcg cagccgccgg
      781 atcagtgtcc agctttcttg ttatatagag cgaaatcata attatgattg ccatgataag
      841 cgatattgca cttccgaagc tccagtttcc ggctgtaagg aactgtgttt ctattacgtt
      901 tccgataagc acatactgac cgccgccgag cagcttcggt ataaagaagc ttgatactgc
      961 cggaagaaaa acaagcgtta ttccacttat tactccggga agactcatcg gaaaggttat
     1021 tctccagaag cactggtatt tgtttgctcc gaggtcgctt gccgcctcga tatagctctt
     1081 gtccattttt tcaagtgaag aataaatctg caaaatcata aacggaacaa agttataaac
     1141 catgccgagt ataacggcaa aattagtata tattattttt accgtaccta ttccgaaaag
     1201 accgagtatt gtatt
//
LOCUS       DBMDVO010000538         1211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53800_length_1211_cov_1.317117, whole genome shotgun sequence.
ACCESSION   DBMDVO010000538 DBMDVO010000000
VERSION     DBMDVO010000538.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53800_length_1211_cov_1.317117"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="ACZPE2_07270"
     CDS             <1..1106
                     /locus_tag="ACZPE2_07270"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1319683.1"
                     /translation="NGVITSDKRIVASLPTIQYLIKNGAKVILCSHLGRPKGQVNPEF
                     SMKPVAARLSELLGLKVKMADDVVGPSAQALAASLKDGEVMLLENVRFEPGETKNDPE
                     LSKAFASLADLYVNDAFGSAHRAHSSTAGVAAYLPAACGYLIQKEIKFIGGALENPKR
                     PLVAILGGAKVSDKIGVITNLIDKCDTLIIGGGMAYTFMKALGHSIGDSLLEADKVDL
                     AKEMMDKAKAKGIKFLIPVDNKVGKEYKPDTEAMVVNSDEIPDGWMGLDIGPKTQQLF
                     ADAVKDAGTVIWNGPMGVSEWDNFAAGTIAVATAIADSNAISIIGGGDSAAAIQKLGF
                     ADKMSHISTGGGASLEYLEGKELPGIAALNDKK"
BASE COUNT          343 a          251 c          285 g          332 t
ORIGIN      
        1 aaaacggcgt tataacaagc gacaagagaa tagtagcttc tcttcccaca attcagtatc
       61 ttataaagaa cggagccaag gttatccttt gctctcatct cggcagaccg aaaggacagg
      121 taaatcctga gttttctatg aagcccgttg cagctcgtct ctctgagctt ctcggcctga
      181 aagttaaaat ggctgacgat gttgtcggcc cgagcgctca agctcttgcc gcctcgctta
      241 aagacggcga agtaatgctt cttgaaaatg ttcgttttga acccggcgag actaaaaatg
      301 atcccgagct ttcaaaggct tttgcatctc ttgcagatct ttatgtaaat gatgctttcg
      361 gttctgcgca cagagctcat tcttcaactg ccggcgttgc cgcatatctt cccgcagcat
      421 gcggatatct tattcagaaa gaaattaagt ttataggcgg agctttggaa aatccgaaga
      481 gaccgcttgt tgcaattctc ggcggagcaa aggtttctga taaaatcggc gtaatcacaa
      541 atttaattga caagtgtgat actcttatca tcggcggcgg tatggcttat acatttatga
      601 aagctctcgg tcacagtata ggtgattctc ttctcgaggc tgataaagtt gaccttgcta
      661 aggaaatgat ggataaggct aaagctaaag gtattaaatt ccttattccc gttgataata
      721 aggtgggtaa agagtataaa cccgataccg aagcaatggt tgttaattca gacgaaattc
      781 ccgacggttg gatgggactt gacataggac ctaagactca gcagctcttt gccgacgctg
      841 ttaaagatgc cggtaccgta atttggaacg gacctatggg cgtttctgag tgggacaact
      901 ttgcagccgg aacaatagcg gttgctaccg ccattgctga tagtaacgcc atttcgatta
      961 taggtggcgg agactctgcg gcagccatac agaagcttgg ctttgctgat aagatgtctc
     1021 atatatcgac cggcggcggt gcttcgcttg aatatcttga aggcaaagaa cttcccggta
     1081 ttgcagctct taacgacaag aaataataaa aatacaggtg agtattttta ctcacctgtt
     1141 aattatgata aaggagaaat aattatgaat aaagcacttc gtaaagctgc tatcgcaggt
     1201 aactggaaaa t
//
LOCUS       DBMDVO010000539         1209 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53926_length_1209_cov_1.316787, whole genome shotgun sequence.
ACCESSION   DBMDVO010000539 DBMDVO010000000
VERSION     DBMDVO010000539.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53926_length_1209_cov_1.316787"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="ACZPE2_07275"
     CDS             complement(<1..204)
                     /locus_tag="ACZPE2_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1319684.1"
                     /translation="MNVYDFDNTIYDGESGFDIFMFYLKKDPKEIAKLIPRFGEAFIR
                     YKRGAIKTDEVIEQYGDMLTDYCV"
     gene            complement(320..1174)
                     /locus_tag="ACZPE2_07280"
     CDS             complement(320..1174)
                     /locus_tag="ACZPE2_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319685.1"
                     /translation="MAETKSCCNDKVSGKIREAVCIDTNRVYDSCADKDCLADLRVYF
                     PPQYQQTIDRATNVRCRGCEILNVFIDVERVPFNRGFYSVDITFFFKVTLDAYSVAAA
                     PPETVCGFTTFSKKCILYGSEGKVRVYSSEFRADSGDRQIIPQNTNPRAKIQVADPIC
                     LEARLCRPCDCCDCLGDSCIPVPSCVKCAFGDEFPDDFGHRPVEKAVAVTIGIFTIVQ
                     LERDVQMLIPAYDFCIPDKECSCDTDDPCEAFRKIQFPVDEFFPPNETKLQTGVSTAT
                     CCGCERSD"
BASE COUNT          294 a          304 c          284 g          327 t
ORIGIN      
        1 cacgcagtag tcggtgagca tatcgccgta ttgttcgata acctcgtcgg tttttattgc
       61 acctcttttg tagcgtatga acgcctcgcc gaaacgcggt atgagcttgg caatttcttt
      121 cggatccttt ttaagataga acataaaaat gtcaaatccg ctctcaccgt catatatggt
      181 gttgtcaaaa tcgtatacgt tcacagcttt tccctcttta cacgaaaaat acatcatgtt
      241 tataacaatg gaattttagc attacaaatc taaaaagtca aggaagccga accatacagg
      301 atcggcttct ttgttttgct taatcgctgc gttcacatcc gcagcacgtc gcggtgctta
      361 cgccggtctg aagcttagtc tcgttgggcg ggaaaaattc gtccacgggg aactgtattt
      421 tcctgaaggc ctcgcacgga tcgtccgtgt cacagctgca ttccttatcg ggtatgcaga
      481 agtcgtatgc cggtatcagc atctggacgt cgcgctcaag ctgaactatc gtgaaaatac
      541 ctattgtaac cgcaacggct ttttcgacgg gacggtggcc gaaatcgtcc gggaattcgt
      601 cgccgaacgc gcatttcacg caggacggga caggaatgca cgagtcaccg agacagtcgc
      661 agcagtcgca cgggcggcag agccttgctt caaggcaaat aggatcggcg acctgtattt
      721 ttgctctcgg atttgtgttt tgcggaatga tttgcctgtc gcccgagtcc gcacggaact
      781 ctgaggagta tactcttacc ttaccctcgc tgccgtagag tatgcatttt tttgaaaatg
      841 tggtaaatcc gcacaccgtt tcgggcggag ccgccgcaac ggaatatgcg tcaagcgtta
      901 ccttgaagaa aaatgtgatg tcaaccgagt aaaagccgcg gttaaacggc actctctcca
      961 cgtcgataaa gacgttaagt atctcacagc ccctgcatct tacattagtc gccctgtcta
     1021 ttgtttgctg gtactgcggc ggaaaataca ccctcaagtc cgcaagacaa tccttatccg
     1081 cacaagagtc gtaaactcgg ttagtgtcta tgcacacggc ttcgcgaatt ttgccggata
     1141 ccttgtcgtt acagcatgat tttgtttctg ccataaaaat ccctcctgta attatttcgg
     1201 cgaccgaat
//
LOCUS       DBMDVO010000540         1208 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_53976_length_1208_cov_1.712737, whole genome shotgun sequence.
ACCESSION   DBMDVO010000540 DBMDVO010000000
VERSION     DBMDVO010000540.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53976_length_1208_cov_1.712737"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="map"
                     /locus_tag="ACZPE2_07285"
     CDS             complement(<1..712)
                     /gene="map"
                     /locus_tag="ACZPE2_07285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR1319686.1"
                     /translation="MIVLKTAKEIELMKEACRISAEALKVAGEAVKPGVTTWEIDHIA
                     YEYIKKHGAEPNFLHLYGFPATACISINDEIIHGIPSKKRVIREGDIVSIDLGAKIHG
                     YNGDNAATFAAGKVSDEAKRLCDTTKESLYKGIEAAVAGGRLGDVGHAVQSYCEDRGY
                     GVVREYTGHGVGTKLHEDPSVPNFGTPGRGVRLLPGMVIAIEPMINEGTAAIKQMPDG
                     WTVKTKDGKLSAHFEHTVA"
     gene            complement(715..>1208)
                     /locus_tag="ACZPE2_07290"
     CDS             complement(715..>1208)
                     /locus_tag="ACZPE2_07290"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HIR1319687.1"
                     /translation="KAKSYIDAGKLVPDEVVIGIIEDRLKAEDCKNGFILDGFPRTIP
                     QAEALDKMGVRIDRVLEIYVPDEKITARLSGRRVCLKCGATYHTEYKKPKTEGVCDVC
                     GEKLVQRKDDMPETVLDRLKTYHEQTEPLKGYYEKKGILRVVEGQEDVADTTALTFKA
                     LED"
BASE COUNT          238 a          337 c          292 g          341 t
ORIGIN      
        1 tcgccacggt gtgctcgaaa tgagctgata gctttccgtc ttttgttttg acggtccaac
       61 cgtcgggcat ctgtttgatt gccgccgtgc cctcatttat cataggctca atggcaatta
      121 ccatgcccgg caacaggcgc acacccctgc cgggagtgcc gaaattcggt acgctgggat
      181 cctcatggag ttttgtacct acgccgtgac cggtatactc tctcacgacg ccgtagcctc
      241 tgtcctcgca atagctttgc accgcgtggc ctacatcacc gagtctgccg ccagcgacag
      301 ccgcttcgat gcccttatac aggctctctt ttgtggtgtc gcacagccgc ttcgcttcgt
      361 cggaaacctt tccggcagcg aaagtagcgg cattatcgcc gttgtagccg tgaattttgg
      421 cgccgaggtc aatactgact atgtcgccct cacggataac acgctttttg ctaggaatac
      481 cgtgaatgat ctcatcattt atggaaatgc atgcggtggc agggaatccg tacagatgca
      541 agaaattcgg ctcagcacca tgtttcttta tatactcata cgcgatgtgg tcaatctccc
      601 aggtagtaac gcccggttta accgcctcgc ctgccacttt taatgcctcc gctgaaatac
      661 ggcaagcctc tttcataagc tcgatttctt tcgcggtttt cagcactatc atgcttaatc
      721 ctccaatgct ttgaaggtga gagctgtggt atcggccaca tcttcctgac cttccactac
      781 gcggagaatg cctttcttct cgtagtaacc cttcaacggt tcggtttgct catgataggt
      841 tttaagcctg tcgagtaccg tctcgggcat atcgtccttt ctctggacga gcttttcgcc
      901 gcagacgtcg caaacgcctt ccgtctttgg ctttttgtac tcggtatgat aagttgcgcc
      961 gcacttgagg cagactcttc tgcctgaaag tcttgcggtt atcttttcgt ccggtacgta
     1021 aatttcgagg acgcggtcta ttctgacacc catcttatcg agagcttcgg cctgaggaat
     1081 agtcctcggg aagccgtcaa ggatgaagcc gtttttgcag tcctcggcct taagtctgtc
     1141 ttcgataata ccgataacga cctcgtcggg gacgagcttg ccggcatcga tataggactt
     1201 ggctttaa
//
LOCUS       DBMDVO010000541         1208 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54004_length_1208_cov_0.928636, whole genome shotgun sequence.
ACCESSION   DBMDVO010000541 DBMDVO010000000
VERSION     DBMDVO010000541.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54004_length_1208_cov_0.928636"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="ACZPE2_07295"
     CDS             <1..395
                     /locus_tag="ACZPE2_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1319688.1"
                     /translation="LSYLRKNARLYNFPNENIIECDVLSGCPDLPKADLIISNPPYIK
                     SEELPSLQREVGFEPKTALDGGKDGLMFYRAICDRWLPCCKKGGEILLECGDSQGEDI
                     RTIFDGKASSSEIMYDFNHIDRVVKIIV"
     gene            425..1099
                     /locus_tag="ACZPE2_07300"
     CDS             425..1099
                     /locus_tag="ACZPE2_07300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1319689.1"
                     /translation="MLFSILRGAYTPMEILVSVFALAFVIFCTMPVHEYAHALVASKL
                     GDNTPRLSGRLTLNPMAHIDWIGALMIVLVGFGYAKPVPINPRNFKNPKKGMALTAVA
                     GPIANILMAMVFLLFENIFSLFSPYVIVKAFILFFRFAATINVGLAVFNLIPIPPLDG
                     SRILQLLIPDKYYFKFMRYERYIIIAVFVLIFLGVLDKPLAFLENALYYGLDYVIGFP
                     FRALSK"
     gene            1111..>1208
                     /locus_tag="ACZPE2_07305"
     CDS             1111..>1208
                     /locus_tag="ACZPE2_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1319690.1"
                     /translation="MDQISYKLDVFEGPMDLLLSLIAKHKLNIYDIP"
BASE COUNT          331 a          253 c          266 g          358 t
ORIGIN      
        1 cactgtcata tctccgcaaa aatgcgaggc tttacaattt tccgaacgaa aatataatcg
       61 agtgcgatgt gctttcgggc tgtcccgatt taccaaaggc ggaccttata atatcaaatc
      121 cgccgtatat aaaatcggag gaacttccct ctttgcaaag ggaggtcgga tttgagccga
      181 aaacggctct tgacggcggc aaagacgggc ttatgtttta cagagccata tgtgatagat
      241 ggctgccgtg ctgcaaaaaa ggcggggaga tacttttgga atgcggtgac tcgcagggcg
      301 aggacatcag aactattttt gacggtaagg cttcatcgtc ggaaattatg tacgatttta
      361 atcatattga cagggtagtt aaaataattg tataattaca tttctgcaaa aaggagaaaa
      421 gcaaatgctc tttagtatcc tcagaggcgc ttatacgccc atggaaatat tggtaagtgt
      481 ttttgcgctt gcttttgtaa tcttctgcac catgcccgtg catgaatatg cccatgcgct
      541 cgttgcgtca aagctcggcg acaatacgcc gcgtctgtcg ggcagactca ccttgaatcc
      601 tatggcgcat atcgactgga taggcgcgct tatgatagta cttgtcggtt tcggctatgc
      661 aaagcccgtt ccgataaatc cgagaaactt taaaaatccc aaaaagggaa tggcacttac
      721 agccgttgca ggccctatcg caaatatttt aatggccatg gtgtttttgc tttttgaaaa
      781 tatcttttct ctcttcagcc cctatgttat agtgaaggca tttattctgt tcttccgatt
      841 tgccgccact ataaacgtag gcctggctgt gtttaacctt ataccgatac cgccgcttga
      901 cggctcaaga atattacagc tgcttatccc cgataagtat tattttaaat ttatgcgtta
      961 cgagagatat ataatcatag ccgtatttgt gcttatcttt ttaggtgtgc ttgataagcc
     1021 gctggcattt cttgaaaacg cattgtatta cggactcgat tacgtaatag gatttccgtt
     1081 cagagcatta tcaaaataaa ggacgtaaaa atggaccaaa tatcatataa gctcgacgtg
     1141 ttcgaggggc ctatggacct gcttttgagc cttatcgcca aacacaaact taatatatat
     1201 gatattcc
//
LOCUS       DBMDVO010000542         1207 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54075_length_1207_cov_0.707052, whole genome shotgun sequence.
ACCESSION   DBMDVO010000542 DBMDVO010000000
VERSION     DBMDVO010000542.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54075_length_1207_cov_0.707052"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1207)
                     /locus_tag="ACZPE2_07310"
     CDS             complement(<1..>1207)
                     /locus_tag="ACZPE2_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1319691.1"
                     /translation="YKMGLINGTRTKSSNIVGQTMKLNPHGDATIYETMVRLTEGNGS
                     LLLPFVDSKGNFGKQYSRDMAYAAARYTEAKLAGICNEVFGDIDKNAVDFVDNFDATM
                     KEPVLLPTAFPNILVNPNQGIAVGMASSFCSFNLAEVCEATIAYMSDPDCNILDYIKA
                     PDFPHGGEFIYNENELSNIFETGRGSFKVRAKYRFDKKNSCIEIYEIPYSTNIETIID
                     KVILLVKSGKIRDITDIRDETDLKGLKIAIDIKRSADPEMIMTKLFSLTPLCDSFSCN
                     FNLLIDGRPQTLGIKGILKEWVRFRMDCIKRRTKYDLDKKNTKLHLLQGLSKILLDID
                     KAISIIRNTEVDAMVVPNLMSGFEIDEQQAEFVAEIKLRNLNREYILKRTDEIKSLRE
                     DIETLKGIYE"
BASE COUNT          298 a          246 c          263 g          400 t
ORIGIN      
        1 tttcgtatat tcccttaagc gtttcaatat cttctctgag gctttttatt tcgtctgtgc
       61 gctttaaaat atattcacgg ttaagatttc tgagctttat ttccgcaacg aattccgcct
      121 gctgttcgtc aatttcaaaa ccggacataa ggttgggaac aaccattgca tcaacctctg
      181 tgtttcggat tatcgaaatc gccttgtcta tatcaagaag aattttggaa agtccctgta
      241 ataaatggag ttttgtgttt ttcttgtcca gatcgtattt tgtgcggcgt tttatgcagt
      301 ccatacggaa acgaacccat tccttgagta tgcctttaat gcctaatgtc tgcggtctgc
      361 cgtcgataag cagattgaag ttacagctaa aggaatcgca aagcggcgta agcgaaaaca
      421 gctttgtcat aatcatttcc ggatcggctg aacgctttat gtctattgct attttaaggc
      481 ctttgaggtc ggtttcgtca cgaatgtcgg ttatgtcacg aattttgccg gatttaacga
      541 gaagtataac cttgtcgatt atcgtttcaa tattggtaga atagggaatt tcatatattt
      601 ctatacagct gtttttcttg tcaaagcggt attttgcacg aaccttaaag ctgcctctgc
      661 ctgtttcgaa tatattggaa agctcgttct cgttatatat aaattctcct ccgtgaggaa
      721 aatcgggagc ttttatatag tcgagaatgt tgcagtcggg atctgacata taggctattg
      781 ttgcctcaca tacctctgca aggttgaagc tgcaaaagga gcttgccatg cctacggcaa
      841 taccttggtt cggattgacg agaatgttcg gaaatgcggt cggaagaaga acaggctcct
      901 tcattgttgc atcaaagtta tcaacaaagt caacggcgtt tttgtctatg tcgccgaata
      961 cctcattgca tattccggca agctttgcct cggtatatct ggcggcggca taagccatat
     1021 cccttgaata ctgctttccg aagtttccct ttgaatcaac gaatggcaga agaagagagc
     1081 cgttgccttc ggtaagacgt accattgttt cgtatatggt ggcgtcacca tgcggattga
     1141 gcttcattgt ctgacctaca atgtttgagg actttgttct tgttccgttt ataagtccca
     1201 ttttgta
//
LOCUS       DBMDVO010000543         1203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54325_length_1203_cov_0.907441, whole genome shotgun sequence.
ACCESSION   DBMDVO010000543 DBMDVO010000000
VERSION     DBMDVO010000543.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54325_length_1203_cov_0.907441"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1203)
                     /gene="rsxC"
                     /locus_tag="ACZPE2_07315"
     CDS             complement(<1..>1203)
                     /gene="rsxC"
                     /locus_tag="ACZPE2_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1319692.1"
                     /translation="DKAIEVILPKENAEMFYPMSQHIGAPCEPIVAVGDYVKAGQKIA
                     ETGAFMSSPIFSSVSGTVKDIRPMLVPGGATVKSIVIENDGKFEMADGIGTKLDYENM
                     SNAEICAAIKAAGVVGLGGAGFPTHIKLAPPPDKKIDYIIINAAECEPYLTCDYRIML
                     EQPEKIIRGLKIMLKLHPNAKGVIGIENNKPEAIKVMSELVKDIPNIEVAELLMKYPQ
                     GAEKQLIYSITKREVPSTTPPSLPADVGCIVDNVATVMAIGNAIYESKPLMDRVITVS
                     GGAAKNPGNYQIKIGMKISDLMEAIGGFSVEPAKFIAGGPMMGPSMYTLDVASVKTTS
                     GLLCFTKEVAYIPPERGCIRCGKCVENCPMGLMPFELNRLAIKGLAEEFGNNHGMDCI
                     ECGSCSFE"
BASE COUNT          324 a          273 c          262 g          344 t
ORIGIN      
        1 actcgaatga acatgaaccg cattcgatac agtccatacc atggttgttg ccgaactctt
       61 cagcaagtcc tttaatagca agtctgttga gctcgaaggg cataagaccc attggacagt
      121 tctctacgca cttaccgcaa cggatacatc ctctttcggg aggaatataa gctacttctt
      181 tagtaaagca aagaagacct gaagttgttt taacagaagc aacatcaaga gtatacattg
      241 aaggacccat catagggccg ccggcaatga atttagcagg ctcaactgag aatccgccga
      301 ttgcttccat aagatcagaa atcttcatac cgatcttgat ctgatagttt ccggggttct
      361 ttgcagcacc gcctgaaact gtgataactc tgtccataag aggtttactc tcatagatag
      421 cattaccgat agccataact gtagctacgt tatctacaat acatccaacg tcagcaggaa
      481 gtgacggagg agtagttgaa ggtacttctc tctttgtgat tgagtagatg agctgtttct
      541 cagcaccctg aggatacttc ataagaagct ccgcaacttc gatgttggga atatccttaa
      601 cgagctcgct cattaccttg atagcttcgg gcttgttgtt ctcgataccg ataacaccct
      661 tagcattggg atgaagtttg agcatgatct taagacctct gatgatcttt tcgggctgct
      721 caagcattat acgatagtcg catgtaagat aaggctcaca ttcggctgca ttaataataa
      781 tgtagtcgat ctttttgtcc ggcggaggag caagtttgat atgtgtcggg aatccggcac
      841 caccgagacc tacaactccg gcagccttga ttgctgcaca gatttctgcg ttggacatgt
      901 tttcataatc cagttttgtt ccgataccgt ctgccatttc aaatttaccg tcattttcaa
      961 taacgataga ttttactgtt gcaccgccag gtacaagcat aggacgaata tcctttactg
     1021 ttcccgaaac gcttgagaaa atcggtgatg acatgaatgc accagtttca gcgattttct
     1081 gtcctgcctt tacatagtcg ccaactgcaa cgatgggctc acagggagca ccgatgtgct
     1141 gggacatagg ataaaacatt tcggcatttt cttttggaag aatcacttcg atggctttgt
     1201 ctg
//
LOCUS       DBMDVO010000544         1200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54514_length_1200_cov_0.947225, whole genome shotgun sequence.
ACCESSION   DBMDVO010000544 DBMDVO010000000
VERSION     DBMDVO010000544.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54514_length_1200_cov_0.947225"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(20..>1200)
                     /locus_tag="ACZPE2_07320"
     CDS             complement(20..>1200)
                     /locus_tag="ACZPE2_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1319693.1"
                     /translation="TWYYVENGVLNWNFSGAVLYGKTLYYVNGGRITWDYNGTADYNG
                     VKYIFVGSIAQNGIYKSKYTDYNLVYADGKTGWYDYGDNTYYIGSDGRPLCGNQYIDG
                     KRYFFNANGAKASLFGADFSKHQGTIDWASVKQSGVEFVILRAAVRGYGSSGNLVTDS
                     QIAANIKGALSQNIDVGIYVFSQAVTTEEAVEEAERALDIIKGYDIKLPIYFDSEYSG
                     APNRTGRADGLTKAERTSLAIAFCETVRKAGYKAGVYASKSFFYDNLDYAALQSRGYE
                     IWLAHYISSVTDFKYPYNIWQYTSKGSIGGVQSEYADLDIAYYDYANDSDMSERGKNV
                     MVTASSDDFLSFVNTEEKITRYIKTGLASDKEEALRAALLVTNQNASKALIDAINKLS
                     "
BASE COUNT          311 a          303 c          214 g          372 t
ORIGIN      
        1 atatttaatt atgttttatt cagctcaatt tatttattgc gtcaataagg gcttttgaag
       61 cgttttgatt tgttacgagc aaagcggctc ttaacgcctc ctccttatcc gaggcaagcc
      121 ccgtttttat gtaacgtgtt atcttttcct cggtgttaac aaacgacagg aaatcgtcgg
      181 acgatgccgt taccataaca ttttttcctc tctccgacat atcgctgtca ttcgcgtaat
      241 cgtagtaagc tatgtcgaga tcggcatatt cgctttggac gccgcctatg cttcccttgc
      301 ttgtatactg ccatatatta taaggatatt tgaaatccgt cacggacgaa atataatgcg
      361 caagccaaat ttcgtatccc ctgctttgca aagcggcata atcaagatta tcataaaaga
      421 atgatttgct cgcatatacg cctgccttat agcctgcctt tctgactgtt tcgcaaaaag
      481 ctatcgccaa agaagtacgc tccgcttttg taaggccgtc cgctctgccc gtgcggtttg
      541 gagctcccga atactcggaa tcgaaatata tgggcaattt aatatcgtag cccttaatta
      601 tatcaagcgc tctttccgct tcctccacag cttcttcggt cgtaacagcc tggctaaaca
      661 cataaattcc gacgtcgata ttctgagaca atgctccttt aatgtttgcc gctatttgag
      721 aatccgtcac caaattaccc gaagaaccat agccgcgcac cgctgctctg agaattacaa
      781 attccacacc cgactgctta acacttgccc aatctattgt accctgatgc tttgaaaaat
      841 ccgctccgaa aagcgatgcc tttgcaccgt ttgcattaaa gaaatatctt ttgccgtcta
      901 tatattggtt tccgcacaac ggtctgccgt cacttcctat ataatatgta ttgtcaccgt
      961 aatcatacca acccgtttta ccgtcggcgt aaacgagatt ataatcggtg tacttagact
     1021 tatatattcc gttttgagca atgctcccga caaaaatata tttgacgccg ttgtaatccg
     1081 ccgtaccgtt ataatcccat gttatcctgc cgccgtttac atagtagagt gttttgccat
     1141 ataaaaccgc tccgctgaaa ttccaattca aaacgccgtt ttctacgtaa taccaagtat
//
LOCUS       DBMDVO010000545         1200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54519_length_1200_cov_0.909008, whole genome shotgun sequence.
ACCESSION   DBMDVO010000545 DBMDVO010000000
VERSION     DBMDVO010000545.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54519_length_1200_cov_0.909008"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(369..767)
                     /gene="rpsI"
                     /locus_tag="ACZPE2_07325"
     CDS             complement(369..767)
                     /gene="rpsI"
                     /locus_tag="ACZPE2_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1319694.1"
                     /translation="MYETNPYFYGTGRRKKSVARVRVYAGSGKITINNRDIDDYFGLE
                     TLKLIVRQPLALTGTADKFDIVCNVQGGGVTGQAGAIRHGLSRALLQYDENLRPALKK
                     AGFLTRDPRMKERKKYGLKGARRAPQFSKR"
     gene            complement(787..>1200)
                     /gene="rplM"
                     /locus_tag="ACZPE2_07330"
     CDS             complement(787..>1200)
                     /gene="rplM"
                     /locus_tag="ACZPE2_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1319695.1"
                     /translation="PKAADISRNWYVLDADGKTLGSVAAEAATLLRGKHKPTFAPHAD
                     CGDHVIVINAGKIVLTGNKLEKKMYYHHTGYIGNMKKIQYKTLMAEKPEFALKKAVKG
                     MLPDNSLGRKQLTRLRIFKDGEHTHEAQKPEVKEF"
BASE COUNT          293 a          275 c          273 g          359 t
ORIGIN      
        1 aggctgtttt tttgttaaat aaaagacaat ttcccgacaa cgctttttgt ctcgccatcg
       61 tacaggacaa aaaccgcaag ctctttttat cgcggaggca attcattgta cattgcctca
      121 aaaagcgcgg ttttcaaaat acgctccttt gtatctcccg tcataaattc acttccaaat
      181 agtagtagcc gttcggttac ccgtcgaggc ttttttggga aaattttaaa acgcagcgaa
      241 ccgaacgcaa aatccttttc tcgggctatg caaagaagcc gttatggtat cgctatataa
      301 aaaaaagcca cggaaaaccg ttgatttccc gtggcttttg aagtatttga ttgaaaaatc
      361 gtaaccgatt atctctttga gaactgcggt gcgcgacgag cgcctttgag accgtatttc
      421 tttctctctt tcattctcgg gtcacgagtc aggaagccgg ccttcttgag tgccggacga
      481 agattttcgt cgtactgaag aagagctctt gaaagaccgt ggcgaattgc gcctgcctgg
      541 cctgttacac cgccgccctg aacgttgcag acgatgtcga atttgtcagc tgtgccggtg
      601 agagcgagag gctgacgaac tataagttta agtgtttcaa ggccgaaata atcgtcgata
      661 tcacgattgt ttatcgttat tttgcctgag cctgcataaa cgcgaactct tgcaaccgac
      721 ttctttcttc tgcctgtgcc gtaaaaataa ggattagtct cgtacattta tttttgcctc
      781 cctttctcag aattctttaa cttcgggctt ctgagcctca tgtgtgtgtt cgccgtcttt
      841 gaagatacga agtcttgtaa gctgctttct gccgagtgag ttatcgggaa gcataccctt
      901 aactgccttt ttaagagcaa attcgggctt ttcagccata agggtcttat actgaatctt
      961 cttcatgtta ccgatataac cggtgtgatg atagtacatt ttcttttcaa gcttgttgcc
     1021 tgtaagaact attttgccgg cgttgattac aattacgtga tcgccgcagt cagcgtgagg
     1081 agcaaatgtc ggcttgtgct tgccgcgaag aagcgtagca gcctctgcag caacggaacc
     1141 gagggttttg ccgtcagcat caagaacata ccaattacgg ctgatgtcgg cagccttagg
//
LOCUS       DBMDVO010000546         1198 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54635_length_1198_cov_1.110301, whole genome shotgun sequence.
ACCESSION   DBMDVO010000546 DBMDVO010000000
VERSION     DBMDVO010000546.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54635_length_1198_cov_1.110301"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="ACZPE2_07335"
     CDS             complement(<1..883)
                     /locus_tag="ACZPE2_07335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR1319696.1"
                     /translation="MNLFKRQSYLRKLYFHPGEREALAKLFSDAMNREDSAQRFSALI
                     SGLDNESRNTVSDIIHRMGMIADGNKSLQDVYTQREQEEFVRMNDEFSSKIVKLNDNL
                     YYYNGYYLPVNQFDSSVFFTRYGIDKLTTLDSVRNKHIIDAGGYVGDTALLFSSYTDK
                     SIHVFEASPSNMDIIRETIRLNQLENIVPVSKALGEKSGTATFSLGERNSCNSLVERP
                     GYNYPNHIEVSVITLDDYVRENNLEVGLIKVDIEGGEQLLLKGAVETIRTQHPILLIS
                     IYHSANDFFEIKPMIEKM"
BASE COUNT          302 a          285 c          250 g          361 t
ORIGIN      
        1 acattttctc tatcataggc ttaatctcaa agaaatcgtt tgccgaatga taaatgctga
       61 tgagaagtat cgggtgctga gtgcggatgg tttcgacagc gcccttgagc agcagctgct
      121 cacctccctc gatgtcaacc tttataagac cgacctcgag attgttttcc cgtacataat
      181 catcaagagt aataacggaa acttcgatat gattcggata attataaccg ggacgttcaa
      241 caagggaatt gcaggaattg cgctcgccca gactgaacgt tgccgtgccg cttttctcgc
      301 ccagcgcctt gcttacaggc actatgttct ctaactgatt cagccttatc gtttcacgga
      361 taatatccat atttgaagga gaagcctcaa atacatgaat gctcttatcc gtatatgacg
      421 agaacagaag cgccgtgtca ccgacatatc cgcctgcgtc gattatatgc ttatttctca
      481 cgctgtccag cgtggtcagc ttatctattc cgtatcttgt gaagaatacg ctgctgtcaa
      541 actggttaac ggggagataa taaccgttgt aataataaag attatcgttc aactttacta
      601 tctttgagct gaattcatca ttcatacgaa cgaattcttc ctgctcacgc tgtgtataga
      661 catcctgcaa ggacttgttg ccgtccgcta tcattcccat acgatgaatt atatcggaaa
      721 cggtatttct gctctcgttg tcaagtccgc ttataagtgc gctgaatcgt tgtgcgctgt
      781 cctctctgtt catagcgtca ctgaacaatt ttgccagagc ttcacgttca ccgggatgaa
      841 aatacaattt tctgagatat gactgccttt tgaacagatt catctcacgg cggatcgtat
      901 ttgccgtatt ctctgcatca agcaaagact gtgcgtattc gcccgtcttt gaggttttgt
      961 caaagcctgc ggttacgctg tccaaaagtg acttaatatc agtcgaaatg ctttcgttat
     1021 ctgccgataa gccgttaaat gtatccgcat actcgcctgt tttcgaggtt ttatcaaagc
     1081 ctgcggttac gctgtccgaa agtgatttaa tatcagccga aatgctttcg ttatctgccg
     1141 ataagccgtc aagcacagac acatattcgc cagtctttga ggtcttgtca aagcctgc
//
LOCUS       DBMDVO010000547         1195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54816_length_1195_cov_1.299817, whole genome shotgun sequence.
ACCESSION   DBMDVO010000547 DBMDVO010000000
VERSION     DBMDVO010000547.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54816_length_1195_cov_1.299817"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..>1195
                     /locus_tag="ACZPE2_07340"
     CDS             22..>1195
                     /locus_tag="ACZPE2_07340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR1319697.1"
                     /translation="MLDSSRRFWRTSEIKRLIDLMALHKLNVFHWHLTDDQGWRIEID
                     KYPMLTQKGSHRRRRDRKTNDGKRYYTKEQIRDIVEYCRKRFIKVIPEFDVPGHTVAA
                     IASYPELSCFGQKRNVASSWGVKHDILCAGKDSTLKFVYEVTDEIMQLFPDKIIHIGG
                     DDAVKMRWSICPHCQKRIKDESLKDEQELYTWFMNKIASHIEKNGGKAIIRSCDGSDK
                     EKPLDKNIIWQVCDKDMNGKVVSREGKMGRAFINSSSPHYYLNLPYSMINLKKTYEYA
                     PEPVYGELLGTEAVIWTEHISSIKSLDFMVFPRIAAIAETAWSAKDDRSYERFLNSLP
                     EYYDLLNIYEVRYATLKQANPSKLRKAAYGVAWRNKTVNFHRLYDLAEDEKTRSLAK"
BASE COUNT          360 a          242 c          309 g          284 t
ORIGIN      
        1 ctctatcctt acaggggctt tatgcttgac tcaagccgcc gtttttggcg gacaagcgag
       61 ataaaacgtc ttatagacct tatggcactt cacaagctga atgtttttca ttggcatctt
      121 accgatgacc agggctggcg tattgaaatc gataaatatc cgatgcttac gcaaaagggc
      181 tcgcaccgcc gccgtcgcga cagaaaaaca aatgacggca aaagatatta taccaaggag
      241 cagatacgcg atattgtaga gtattgccgc aagcgcttta taaaggttat tccggaattt
      301 gacgtgccgg ggcatacggt ggcggcaata gcttcgtacc cggagctctc ctgcttcggg
      361 caaaagcgga atgtggcgtc gagctggggc gttaagcacg acattttgtg tgccggtaag
      421 gacagcacgc ttaaattcgt atacgaagtt acggatgaga taatgcagct tttccccgat
      481 aagattattc atataggcgg cgatgacgcc gtgaaaatga gatggagcat atgtccgcac
      541 tgccaaaaac gcataaagga cgagtctctc aaagacgagc aagagctata cacttggttt
      601 atgaacaaga tagcgtctca tattgagaaa aacggcggaa aggcgataat acgcagctgc
      661 gacgggagcg acaaagaaaa accgcttgat aaaaatataa tttggcaggt ttgcgataaa
      721 gatatgaacg gcaaagtcgt aagccgagaa ggcaaaatgg gcagagcttt tataaattcg
      781 tcctcgccgc attattatct taatttgccg tacagtatga taaatctcaa aaagacgtat
      841 gaatatgccc cggagcctgt ttacggcgag cttctgggga cagaagcggt tatttggaca
      901 gagcacataa gcagtataaa atcgcttgat tttatggtgt tcccacgtat tgcggcgata
      961 gccgagactg cgtggtcggc taaggacgac agaagctatg agagattttt gaacagcctg
     1021 ccggaatact atgatctttt gaatatatat gaggttcgct atgctacgtt aaagcaggca
     1081 aatccgagta agctcagaaa agcggcatac ggtgttgcgt ggagaaacaa gaccgttaat
     1141 ttccataggc tgtatgacct tgcggaggat gaaaaaacaa ggtctcttgc caaaa
//
LOCUS       DBMDVO010000548         1195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54830_length_1195_cov_1.122486, whole genome shotgun sequence.
ACCESSION   DBMDVO010000548 DBMDVO010000000
VERSION     DBMDVO010000548.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54830_length_1195_cov_1.122486"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="ACZPE2_07345"
     CDS             complement(<1..>1195)
                     /locus_tag="ACZPE2_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1319698.1"
                     /translation="KVYVDIENYVVGAINLIDMRVPGSKEFLDSVDTAEAYLDREFYG
                     KYCKKEDVNAVCIGHTHIDVAWLWTLAQTREKVLRSFSTVLELMKKYPEYKFMSSQAQ
                     LYKFLKEESPELYAEVKEMVRLGRWEVEGAMWVEADCNLSSGESLVRQILYGKSFFKN
                     EFGVDSKVLWLPDVFGYSAALPQILRKSGVDKFVTSKIGWSETDKMPYDTFFWKGIDG
                     TDIFTYFMTAQDKVRGKKPATNVTYNAKLNPCQLIGGYDRYQQKDINNEVMITFGYGD
                     GGGGPIRKDLEYYDVLKKGISGVPCAKMEFAGSFLERIKKRAEKNPKTPCWQGELYLE
                     YHRGTYTSMAQNKKLNRRSEFLYENAEMLSVTAQKLLGKEYPREALHGGWETILLNQF
                     HDIIPG"
BASE COUNT          261 a          304 c          273 g          357 t
ORIGIN      
        1 gccgggtatt atatcgtgga actgatttaa aagtattgtc tcccagccac cgtgaagagc
       61 ctccctcggg tactctttgc cgagcagctt ttgcgccgtc actgacagca tttcggcatt
      121 ttcataaagg aattcgcttc ggcggttcag ctttttattc tgcgccatgg aggtataggt
      181 tccgcggtga tattcaagat acagttcgcc ttgccaacaa ggagttttcg gatttttttc
      241 ggctctcttt ttaattcgct cgaggaatga gccggcaaat tccattttag cgcacggtac
      301 tcccgaaatt ccctttttca gcacatcgta atattcaagg tcttttctta taggtccgcc
      361 gccgccgtcg ccgtaaccga aggttatcat cacttcgttg tttatatcct tttgctgata
      421 gcggtcgtat ccgcctatga gctggcaagg gttgagtttt gcgttgtaag tgacgtttgt
      481 cgccggcttt ttacctctga ccttatcctg cgcggtcata aaatacgtga atatatcggt
      541 accgtctatc cccttccaga agaaggtgtc atacggcatt ttatcggtct cgctccagcc
      601 gattttggag gtcacgaatt tatcaacgcc gctcttacgg aggatttgcg gcagtgccgc
      661 ggaataaccg aatacatcgg ggagccaaag cacctttgaa tcaacgccga attcgttttt
      721 gaagaagctc ttgccgtata gtatctgcct tacaaggctc tcgcccgacg acaggttgca
      781 gtccgcctcg acccacatgg cgccctcgac ctcccatctg ccgaggcgta ccatttcttt
      841 tacctcggcg taaagctcag gactttcctc tttcaggaat ttatagagct gcgcctgact
      901 cgacataaac ttgtattcgg gatatttttt cataagttca agtacggtcg aaaaagagcg
      961 cagcaccttt tccctcgtct gtgcaagagt ccagagccaa gctacgtcta tatgcgtatg
     1021 gcctatgcag acggcgttta cgtcttcttt tttgcagtat ttgccgtaga attccctgtc
     1081 gagatatgct tcggcagtat cgacggagtc aagaaattct tttgaaccgg gcacgcgcat
     1141 atcaatgaga tttattgcgc cgacaacgta gttttcaata tcgacataga ccttt
//
LOCUS       DBMDVO010000549         1195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54836_length_1195_cov_1.071298, whole genome shotgun sequence.
ACCESSION   DBMDVO010000549 DBMDVO010000000
VERSION     DBMDVO010000549.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54836_length_1195_cov_1.071298"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="ACZPE2_07350"
     CDS             complement(<1..888)
                     /locus_tag="ACZPE2_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1319699.1"
                     /translation="MIYFDNAATTYPKPPEVIKTMSRATVRYGANPGRGGHKMSYLTS
                     EAVYETRKRVAEFFGTDAPENVIFTKNCTEALNIVIKGLCVPGCRFICSSLDHNAVIR
                     PLEALRRSGTADYDIAPVGKTDDETVADFKRLFRSNTRAVICTGASNVFGERLPTAKL
                     ARLAHENGALFILDAAQTAGIVNIDMRRDEIDFLCTAGHKGLYGPMGTGLLVINSDEH
                     LYSLIEGGTGSFSAVLSQPEIYPDRFESGTLNVPGILSLGEGIRFVSDRGVSRIYKSE
                     QGHIADIYKALKNIPNVRLY"
     gene            complement(885..1106)
                     /locus_tag="ACZPE2_07355"
     CDS             complement(885..1106)
                     /locus_tag="ACZPE2_07355"
                     /inference="COORDINATES: protein motif:HMM:NF023251.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Se/S carrier-like protein"
                     /protein_id="HIR1319700.1"
                     /translation="MNRLIIKVGSVTFALKARDILSARNIRSFVRKTPNPSKGEGCGY
                     SLVVPNATESVIGVLRLSGIEILETQWEK"
BASE COUNT          311 a          300 c          257 g          327 t
ORIGIN      
        1 atacaggcga acgttcggaa tattttttaa tgctttatat atatccgcta tatgcccttg
       61 ttcgctttta taaatccgcg agacgccgcg atccgaaaca aagcgtatcc cctcgccgag
      121 agagagtatt cccggtacgt taagagttcc gctttcaaat cggtcgggat agatttcggg
      181 ctgtgaaagg actgctgaaa agctgcccgt accgccctca ataagagaat agagatgttc
      241 gtcggaattt atcacaagaa gccctgtgcc cataggaccg taaaggccct tatgccctgc
      301 cgtacacaaa aaatcaattt catcacgtct catatctata ttgacaatac ctgccgtctg
      361 tgcagcgtca agtataaaca aggctccgtt ttcatgggca agccttgcga gctttgccgt
      421 agggagtctc tcgccgaaaa cgtttgacgc gccggtacaa attacagccc ttgtattact
      481 tctgaacagc cttttgaaat cagccacggt ttcatcatcg gttttgccga caggggcaat
      541 atcatagtcg gctgttccgc tacgccttaa cgcctcaagc ggccttatta cggcgttatg
      601 gtcaagactc gagcatataa aacggcaccc cggcacacaa agacccttta taacgatatt
      661 cagagcctcg gtgcaattct ttgtaaatat tacgttttcg ggagcatccg taccgaaaaa
      721 ttcggcaacc cttttacggg tttcgtatac cgcttcggag gtgagatatg acattttgtg
      781 accgcctctg cccggatttg caccgtaccg aaccgttgca cggctcattg tttttataac
      841 ctcgggaggt ttgggataag tggtggcggc attatcaaaa tatatcattt ttcccactgc
      901 gtttcgagta tttctatgcc tgagagtctt agtaccccta tgaccgactc cgtggcattc
      961 ggaacgacaa gagaatagcc gcacccctcg ccctttgacg gattgggagt ttttctcaca
     1021 aaagaacgga tattccgcgc cgataaaata tcacgcgcct ttaaagcaaa ggtgacagag
     1081 ccgaccttta ttatcagcct attcatatat atttcattcc cctcggataa agaaattaaa
     1141 cacacttgtc ttttacatat tatgcgtttt accgcgccgc ggtgaaacat aaaat
//
LOCUS       DBMDVO010000550         1193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_54951_length_1193_cov_1.047619, whole genome shotgun sequence.
ACCESSION   DBMDVO010000550 DBMDVO010000000
VERSION     DBMDVO010000550.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54951_length_1193_cov_1.047619"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="ACZPE2_07360"
     CDS             complement(<1..611)
                     /locus_tag="ACZPE2_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HIR1319701.1"
                     /translation="MTNKKIISPANEAFSYMGRIDFSKPDSPLFIYAGSMVSAKFTGT
                     SVGMMIFPHLIYKVTWIGVIVDGIQYKFELNHSDTPVYIPIIDGLDEGEHAITIYKRT
                     AGSHHYFNFCGLMIDENAEVRAENHEYDMNIEVYGDSVSAGEVVEAVNYCEHTDPSHI
                     SQFDNSWFAYSLQLARKLNARINCNAQGGISLINGAGYFDNYIG"
     gene            705..>1193
                     /locus_tag="ACZPE2_07365"
     CDS             705..>1193
                     /locus_tag="ACZPE2_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1319702.1"
                     /translation="MKKRILAVLIATALAVSVTGCAGNTTSSSDSTSASSVAESSDTS
                     TQTSADASSEDESKDESSQEESKDLEANYTKYTLDGDLTQHMIDMSRLNEGNKVRLAN
                     VIKKLKNGEEVTVGFIGGSITQGTSAGNDLCYAKLTADWLQQTYSNAKINYVNAGIGA
                     TGS"
BASE COUNT          348 a          279 c          257 g          309 t
ORIGIN      
        1 ccgatataat tatcaaaata tcctgcgccg ttgataagcg aaattccgcc ctgtgcattg
       61 cagttgatac gagcgttcag ctttcttgca agctggagag aatatgcaaa ccagctgttg
      121 tcgaactgac tgatatgtga ggggtctgta tgctcgcaat agtttacagc ctctactacc
      181 tcacctgccg aaacgctgtc gccgtaaacc tcgatattca tatcatattc gtggttttcc
      241 gctcttacct ccgcattctc atctatcata agtccgcaga agttaaaata atggtgtgag
      301 ccggctgttc ttttatatat ggtaatggca tgctctccct cgtcaagtcc gtcgattatc
      361 ggtatatata ccggagtgtc ggaatggttc agttcgaatt tgtactgaat gccgtcaacg
      421 ataacgccta tccaggtaac cttatatata aggtgcggaa aaatcatcat acctaccgaa
      481 gtgccggtaa actttgcgct gaccatactg cccgcgtaaa taaacaacgg gctgtccggc
      541 tttgaaaagt caattcttcc catgtatgag aacgcctcat ttgcagggct tattatcttc
      601 ttgttagtca tttatttaat tcctcgctta aatttatcat agtgccgaaa ttaaatcggt
      661 ttatatttat tttaacatat cagcggaata ttttcaaccg aattatgaag aaaagaatac
      721 ttgcggtact tatcgcaacg gcgcttgccg tatccgtaac aggctgtgcg ggcaatacga
      781 caagcagcag cgacagcacc tccgcttcat cggttgcgga aagctcggac acaagtacac
      841 agacatctgc agacgcatct tcagaagatg aaagcaaaga cgaaagctca caggaggaat
      901 ccaaggactt ggaagcaaac tatacaaagt acacccttga cggcgacctt actcagcata
      961 tgatagatat gtcacgtctt aacgagggca acaaggtaag acttgcaaac gtaataaaga
     1021 aactgaaaaa cggcgaagaa gtaacagtag gcttcatcgg cggctccatc acacagggta
     1081 caagtgccgg caacgacctt tgctatgcaa agctcacggc tgactggttg cagcagacct
     1141 attcgaatgc caaaatcaat tatgtcaacg caggaatcgg cgctacaggc tca
//
LOCUS       DBMDVO010000551         1192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55002_length_1192_cov_1.235564, whole genome shotgun sequence.
ACCESSION   DBMDVO010000551 DBMDVO010000000
VERSION     DBMDVO010000551.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55002_length_1192_cov_1.235564"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1192
                     /gene="guaA"
                     /locus_tag="ACZPE2_07370"
     CDS             <1..>1192
                     /gene="guaA"
                     /locus_tag="ACZPE2_07370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1319703.1"
                     /translation="LGVPILGICYGCQLISWMVGGNVETAEQSEYGKIDLTVTKKQSE
                     IFADVDETSVVWMSHTDRVKVMPSGFEITAKTEACPIAAYENEEKKIYGVQFHPEVTH
                     TQFGFKILHNFLYNVCGCHGDWKMDSFIDDTVKSLREKIGDKKVILGLSGGVDSSVAA
                     GLLSRAVGKQLTCVFVDQGLMRKNEGDFVEKTFTSLFDMNFVRVNCQDHFINALKGVS
                     DPEQKRKIIGTEFYKVFWDEIRKQAEKGFFAQGTIYPYCIESGKGDADKIKTHHNKVK
                     MPDDVEFIDVIEPLSSLFKDEVRAVGEKLGIPHELVWRQPFPGPGLGVRIIGEITKEK
                     IEVLQNADWVLRDEMAKNGYEKNLAQFFCVLPGVNTVGVMGDHRTYDNLVAIRAVTTD
                     DFMTA"
BASE COUNT          336 a          266 c          313 g          277 t
ORIGIN      
        1 ctcggcgtgc ccattctggg catatgctac ggctgtcagc ttatttcgtg gatggtcggc
       61 ggcaatgttg agactgcgga acagtccgaa tacggcaaaa tcgaccttac ggttacaaaa
      121 aaacagtctg aaatttttgc cgacgttgat gaaacgagcg tagtttggat gagccatacg
      181 gacagagtta aagtgatgcc gtcaggtttt gaaataaccg caaaaacgga agcctgcccg
      241 atagcagctt atgaaaacga agaaaagaaa atatacggcg tgcagttcca ccccgaggta
      301 actcacacgc agttcggatt taaaattctt cacaattttc tgtataatgt ttgcggctgt
      361 cacggcgact ggaagatgga cagctttatt gacgataccg taaagagcct tcgcgaaaaa
      421 atcggagaca aaaaggttat tctcggcctt tcgggcggtg tcgattcctc ggtcgcggcg
      481 ggacttctct ctcgcgccgt cggcaaacag ctcacctgcg tttttgttga ccagggactt
      541 atgcgtaaga acgagggcga tttcgtcgaa aagacattca cttcgctgtt cgatatgaat
      601 ttcgtgcgcg taaactgtca ggaccatttt ataaatgctc ttaaaggagt atccgacccg
      661 gagcagaaga gaaaaatcat aggcacggaa ttttacaagg tgttctggga tgagataagg
      721 aaacaggccg agaagggatt ttttgcacag ggcaccatat acccctactg catagagtcc
      781 ggcaagggcg atgccgataa aatcaaaacg catcacaata aggtcaaaat gcccgacgac
      841 gttgaattca tcgacgttat tgagcctctt tcaagcctgt ttaaggatga ggtcagagca
      901 gtaggcgaaa agctcggaat tccgcacgag cttgtctggc gtcagccgtt cccgggaccc
      961 ggactcggtg tcagaattat cggcgagata acaaaggaaa agattgaagt tctccaaaat
     1021 gccgattggg tgctgcgcga tgaaatggcg aagaacggtt atgaaaaaaa cctcgcacag
     1081 ttcttctgtg tgcttccggg agtcaatacg gtaggcgtta tgggcgatca ccgcacatac
     1141 gataaccttg tggcaatccg cgccgtaaca accgatgact ttatgacagc cg
//
LOCUS       DBMDVO010000552         1190 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55160_length_1190_cov_0.864096, whole genome shotgun sequence.
ACCESSION   DBMDVO010000552 DBMDVO010000000
VERSION     DBMDVO010000552.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55160_length_1190_cov_0.864096"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /gene="scpB"
                     /locus_tag="ACZPE2_07375"
     CDS             complement(<1..547)
                     /gene="scpB"
                     /locus_tag="ACZPE2_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1319704.1"
                     /translation="MPVNAKNLTSAAEAVLFSVGEPLEIGKLAEVLSIDEETCRRTLL
                     NLSASLDERSSGICLLRLGDKYQLSSRTEYADLIRSVLEVKKNAPLSNAAFEVLAVIA
                     YNQPVTKAFVEQVRGVDCSGVISTLCQKRLVEEKGRLELPGRPLVYGTTPEFLRCFGV
                     ESLDELPELPKNEPLAEENHEE"
     gene            complement(551..>1190)
                     /locus_tag="ACZPE2_07380"
     CDS             complement(551..>1190)
                     /locus_tag="ACZPE2_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014654.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1319705.1"
                     /translation="VELVDQYLDYVRRMQDEDMYVASEFLEMAARLVYLKTVSLLPVH
                     EEADELKRELTGELIEYRDCKLMAEKLSQRTDGFDHFVREPMKIETDPTYTRVHDGAE
                     LLRAYLAAAGRKLRNLPPPIEAFREIVAKKIVSVGSKIRSIYKKFASVGSKRKFTDLF
                     SDAQSRSDMVATFLAVLELAKDKKVMVSGDGGDTSIELLSADFGDTVSEEWE"
BASE COUNT          295 a          317 c          263 g          315 t
ORIGIN      
        1 tttcttcgtg attttcttct gcgagaggct cattttttgg gagttccggc aattcatcaa
       61 gagattccac accgaaacag cgtaagaatt ccggagtcgt tccgtataca agcggtctgc
      121 cgggaagctc gagtctgcct ttttcctcaa caagtctctt ttggcagagt gttgatatga
      181 caccggaaca atcaacgccc ctcacctgct ccacaaaagc cttggtaacg ggctgattgt
      241 acgctataac agcaagaacc tcaaacgcag cgtttgaaag cggtgcgttc tttttgacct
      301 caagcacaga acgtataaga tcggcatact cggttcgcga cgacagctga tacttgtctc
      361 cgagccttaa aaggcatata ccgcttgaac gttcgtcgag agacgcggac aaattgagaa
      421 gcgtcctgcg gcaggtctcc tcatcgattg agagcacctc ggcaagctta cctatttcaa
      481 gaggttcacc cactgaaaac agaaccgcct cagcggcaga tgtaagattt tttgcgttta
      541 caggcatttt ttattcccac tcctcagaaa cggtatctcc gaaatcagcc gacaaaagtt
      601 ctatgctcgt atcgcctccg tcgccgctga ccataacttt tttatccttg gcaagctcaa
      661 gtacggcaag aaacgtggcc accatatccg aacgcgactg tgcgtccgaa aaaaggtcgg
      721 taaatttgcg tttactgccg acgcttgcga attttttata tatagaacga attttcgaac
      781 cgaccgacac tatttttttt gccactattt cacgaaatgc ctcaataggc ggcggcagat
      841 tgcgaagctt tctgcctgcg gcggcaagat atgctcttaa aagctctgct ccgtcgtgta
      901 ctcttgtata tgtggggtcg gtttctatct tcatcggctc tcgcacaaaa tggtcaaagc
      961 cgtctgtacg ctgagatagc ttttcagcca tgagcttgca gtctcggtac tcgataagct
     1021 caccggtaag ctcccttttc agctcgtccg cctcctcgtg aaccggcaag agagacaccg
     1081 tctttaaata gacgagcctt gccgccatct caaggaattc gcttgccaca tacatatctt
     1141 catcctgcat acggcggaca taatcgagat attggtcaac aagctccact
//
LOCUS       DBMDVO010000553         1189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55183_length_1189_cov_1.304228, whole genome shotgun sequence.
ACCESSION   DBMDVO010000553 DBMDVO010000000
VERSION     DBMDVO010000553.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55183_length_1189_cov_1.304228"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1189)
                     /locus_tag="ACZPE2_07385"
     CDS             complement(<1..>1189)
                     /locus_tag="ACZPE2_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1319706.1"
                     /translation="GPAGLSAAYYLAQMGYKPTVFEKNPIPGGMMTYGIPSYKLEKDV
                     IAAEIDIIKALGAEIKCGVEVGKDVTIDELKKQGYKAFYIAIGCQGGKRPGVKNDDAK
                     GTAIAVEYLRHCFEHREDSFSGNVVVVGGGNVAIDCARNAHRLGATDVKMFCLESRDT
                     MPASNEEILEAEEENVGINPSWGPKEVTVNDKGEVTGIIFKKCLRTIDPETGKFSPVY
                     DENETVEIKADKIVFAIGQAIEWGNLLEGSKVKFWHGNYPVADKFTYETDDPDIFVGG
                     DVFTGPRFVIDAIAAGHEAAESLHRHVRPDASMTIGRDRRNFTPLNKDDLTYPSYDTA
                     GRQEAGMDESIDYKMSYKDAHLDLTEEQVKTETGRCLGCGASYVDPHKCIGCGLCTTK
                     CEFD"
BASE COUNT          250 a          315 c          299 g          325 t
ORIGIN      
        1 gtcaaattca cacttcgtgg tgcaaagacc gcagcctata cacttgtgcg ggtcaacata
       61 cgatgcgccg catccgagac agcggccagt ttcggtttta acctgctcct ctgtcaaatc
      121 gagatgtgcg tctttatagg acatcttgta gtcgatgctt tcatccatac cggcttcctg
      181 acgtcctgcc gtatcataag acggatacgt aaggtcgtcc ttgttgagcg gtgtgaaatt
      241 gcgacggtca cggcctatgg tcattgaagc gtcggggcgt acatggcgat gtaagctctc
      301 agccgcctcg tgaccggctg caatggcatc tattacaaaa cgcggaccgg taaatacgtc
      361 gccgcccacg aatatgtcgg gatcgtccgt ctcatatgtg aatttgtctg caaccgggta
      421 attgccgtgc cagaatttaa cctttgagcc ctcaagcagg ttgccccatt caattgcctg
      481 acctatggca aaaacgattt tgtctgcctt tatttcaaca gtctcgtttt cgtcatatac
      541 gggagagaat ttgccagttt ctgggtctat cgtgcgaagg cactttttaa agatgatgcc
      601 ggtaacctct cctttgtcgt ttacggtgac ctccttcggg ccccaagagg gatttatgcc
      661 gacgttttct tcttcggcct caagtatttc ctcgttgctc gcaggcatgg tgtcgcgcga
      721 ttcaaggcag aacatcttaa cgtccgttgc gccgagacgg tgagcattgc gcgcgcagtc
      781 tatcgcaacg ttaccgccgc cgactaccac aacattaccg gagaatgaat cctcgcggtg
      841 ttcaaagcag tggcggagat attctacggc aatagcggtg ccctttgcat catcgttttt
      901 aacgccggga cgtttgccgc cctgacagcc tatggcgatg taaaatgcct tgtagccctg
      961 ttttttgagc tcatctatgg tgacgtcctt gccgacttca acgccgcact tgatctctgc
     1021 accgagagcc ttgattatgt cgatttcggc agcgataaca tccttttcga gcttgtagct
     1081 cggaataccg tatgtcatca taccgccggg aatgggattt ttttcaaata ccgtcggctt
     1141 ataacccatc tgggcgagat aatatgcggc gctgagtcct gcggggccg
//
LOCUS       DBMDVO010000554         1188 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55237_length_1188_cov_1.326587, whole genome shotgun sequence.
ACCESSION   DBMDVO010000554 DBMDVO010000000
VERSION     DBMDVO010000554.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55237_length_1188_cov_1.326587"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(22..180)
                     /locus_tag="ACZPE2_07390"
     CDS             complement(22..180)
                     /locus_tag="ACZPE2_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1319707.1"
                     /translation="MENKGVKCDVCECVHNTAGCGCDRSVIEVTHDCSCTNQQIQDPH
                     FCKSYEKK"
     gene            complement(307..1089)
                     /locus_tag="ACZPE2_07395"
     CDS             complement(307..1089)
                     /locus_tag="ACZPE2_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1319708.1"
                     /translation="MKCIILTKRGLTAITAVTLSALLIVFGICGRKSALSSAAAEEGK
                     RLLPIYCVETGEKKVAVTFDAAWGADDTERLIEILKNHNAKATFFVVGEWAEKYPDSV
                     KAFYASGHEIGNHSASHNMYSSLTESEIIKDITLCNEAVKKAIGVTPVLLRAPSGDYT
                     NDTITAATKLNMKTIQWSVDSLDWKGLDTDEIVKRVTDKTECGSIILFHNDIKNTPDA
                     LDRILTDLEKKGYSFVTVSELIYDGDYKIDSAGKQIKNAAAN"
BASE COUNT          270 a          275 c          259 g          384 t
ORIGIN      
        1 ttttttcttt aattcgcgtg cttatttctt ttcgtagctt ttgcagaaat gcgggtcctg
       61 aatctgctga tttgtgcatg agcagtcatg agttacctcg ataacgcttc tgtcgcagcc
      121 gcagccggcg gtgttgtgta cgcattcgca tacatcgcat tttacaccct tattttccat
      181 aatgtcacct cctgtaagat gatattatat tttgcgcagt tataaattct ttatacggga
      241 aagagacaag taaaaacgta tttttcggat aaaatttcca atcgaatttt ttatacggcg
      301 gagcgttcaa tttgcggcgg catttttgat ttgcttgccg gctgaatcta ttttgtaatc
      361 gccgtcgtat ataagctcgc ttaccgtaac aaacgaatag ccttttttct caagatccgt
      421 gagaattcgg tcgagcgcgt cgggcgtgtt ttttatatcg ttgtgaaaga gtataattga
      481 accgcactcg gtcttatccg ttacgcgctt tactatttca tcggtgtcaa gtcccttcca
      541 gtcaagcgaa tcgacggacc attgtattgt tttcatattg agctttgtcg ccgcggttat
      601 cgtgtcattt gtataatccc ccgacggagc gcgcaaaaga acgggtgtaa ctcctatggc
      661 tttttttacc gcttcgttac agagtgttat gtcttttatg atttcgctct ctgtaagact
      721 cgaatacata ttgtgcgatg cagagtgatt tcctatctcg tgtccggagg cataaaacgc
      781 ctttacgctg tcgggatatt tttccgccca ttccccgacg acgaaaaacg tagccttggc
      841 attgtgattt tttaaaattt ctatgagcct ttccgtgtcg tcggctcccc aagcagcgtc
      901 aaaggtgact gcgacctttt tttcgcccgt ctcaacgcag tagatgggca gaagccgttt
      961 accctcctcg gcagcggcag agctgagtgc gctttttctg ccgcagattc cgaatacgat
     1021 aagcagcgcg gataatgtga cagcggttat cgccgtaagc cctctcttag tcaaaattat
     1081 acatttcaaa aaaacacccc ctcatactaa atgtatgagg gggacaagtt tttaagactt
     1141 tattcggcta ccggctcgct gtctttttca cggagctcac gaagctct
//
LOCUS       DBMDVO010000555         1186 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55381_length_1186_cov_1.051613, whole genome shotgun sequence.
ACCESSION   DBMDVO010000555 DBMDVO010000000
VERSION     DBMDVO010000555.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55381_length_1186_cov_1.051613"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            210..641
                     /locus_tag="ACZPE2_07400"
     CDS             210..641
                     /locus_tag="ACZPE2_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1319709.1"
                     /translation="MDKVIVVYWSQTGNTEMMANAIGEGAKEAGKEAVVVTPDKASLD
                     DLKAAKAFALGCPAMGAEVLEEAEMEPFVCDVEKFVGGKSIVLFGSYGWGDGQWMRDW
                     VDRMSAAGANILGNEGIMAHETPDEAVLDECKAVGKQLASL"
     gene            complement(802..>1186)
                     /locus_tag="ACZPE2_07405"
     CDS             complement(802..>1186)
                     /locus_tag="ACZPE2_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1319710.1"
                     /translation="CEYCFAHDGVCVQKDDMALVFDELNTADMVVFAAPIYWFDITAQ
                     LKTVIDRLYAHGKIGFNFNKTALLLDAGADGGVFTAAITQYKAMTAFLKWEDKEIITI
                     GGMMGKGDMEKAAKLADVRALGASL"
BASE COUNT          352 a          235 c          260 g          339 t
ORIGIN      
        1 ttgtatacat atattcagtg attgaaaaaa tattcgtttt cgctttttat gaccgcattg
       61 gtcaatttat atgaaagtag gttgacatga tatatatgtt agtatatact aactcatgta
      121 agttagttat acctaataac gacttatctt ccggataccg aagcaacggt attcaaagtt
      181 aataatacat aacaggagga atttttaaaa tggacaaagt aattgttgtt tactggtcac
      241 agacaggaaa cacagaaatg atggcaaatg ctatcggcga aggagcaaaa gaggcaggta
      301 aggaagcagt tgttgtaaca cctgataaag cttctcttga tgatcttaaa gctgctaagg
      361 ctttcgcact cggctgccct gcaatgggag cagaggttct tgaggaggct gaaatggagc
      421 ccttcgtatg tgatgtagaa aaattcgtag gcggcaaaag tatcgttctc ttcggctcat
      481 atggctgggg cgacggacag tggatgcgtg actgggtaga cagaatgagc gcagccggtg
      541 caaacattct cggaaacgaa ggaattatgg cacacgaaac acccgatgag gctgttcttg
      601 acgaatgcaa agcagttgga aaacagcttg catcactctg atatagttag caatattata
      661 tgggcaatat gcccgatttt tccaaactaa acctaaaacc ttataaacct tatacctgat
      721 gtaaactctc tctaagtata aacctctcct tattaacccc caagattttt taaggagagg
      781 tttttcgatt aatatatttg cttaaagact tgctcccaat gcacgtacat cagcaagctt
      841 tgcggctttt tccatatcgc ctttacccat catgccgcct atagtgataa tttctttgtc
      901 ctcccatttt aagaaggctg tcattgcctt gtactgtgtt attgcggcag taaacacacc
      961 gccgtctgca cctgcgtcaa gaaggagtgc tgttttgttg aagttgaagc cgattttgcc
     1021 atgtgcgtaa agtcggtcga taactgtctt aagctgtgca gtaatgtcga accagtagat
     1081 tggtgcggca aaaacaacca tgtcggcagt gttaagctcg tcaaaaacaa gagccatatc
     1141 gtccttctga acgcatacgc cgtcatgtgc aaagcaatat tcgcat
//
LOCUS       DBMDVO010000556         1182 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55584_length_1182_cov_1.349676, whole genome shotgun sequence.
ACCESSION   DBMDVO010000556 DBMDVO010000000
VERSION     DBMDVO010000556.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55584_length_1182_cov_1.349676"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ACZPE2_07410"
     CDS             complement(<1..449)
                     /locus_tag="ACZPE2_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319711.1"
                     /translation="MEEKTLHEHLVAKTDGKAEPDNILIAGSIRITVIFPNLIRVEYG
                     SEKSFCDEATQSVWFRNHGKVDFTYEITKTKIIIKTEKAEFCINRRNGTAIYAKIGDM
                     ESSCDNSENLKGTARTLDQSHGAIPLKDGVISCDGAAVFDDSKSLLM"
     gene            complement(454..>1182)
                     /locus_tag="ACZPE2_07415"
     CDS             complement(454..>1182)
                     /locus_tag="ACZPE2_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319712.1"
                     /translation="SYGDTVSVDLEPHEIRIFKFTNENTAPLKLTEAKFIDEKTVEFR
                     FNSHIAVKASTFTLGGVALKKELRANYSDVRVYLPAEGENLQKLDIDIDVKDIYGNVL
                     SEKVPVTYFKNGCIPISYGVSGRGDFALRLTLSAVPTDGMILLGGKDMSIFVANGKLV
                     FDVKGIKAKSDTIIADKDDVKVYALRERNGMIKLYIDGKLDCSGYDVRNAGADIAAGE
                     IKCGASVKNIEIFNRAFSFDEVKD"
BASE COUNT          297 a          288 c          225 g          372 t
ORIGIN      
        1 cacataagca gcgacttgct gtcatcaaat acggcagctc cgtcacacga gataactccg
       61 tctttgagcg gaatcgcacc gtgtgactga tcaagagtgc gtgccgtgcc ctttaaattc
      121 tcactgttat cacagctcga ctccatatct ccgattttgg catatatcgc cgtaccgttt
      181 ctgcggttta tgcaaaactc ggctttttcg gtttttatta tgattttggt ctttgttatt
      241 tcatatgtaa aatcgacctt accgtgattg cggaaccaaa cactttgcgt cgcttcatcg
      301 caaaaggatt tttcacttcc gtactctacc cttataagat tcggaaatat gaccgttatt
      361 cttatgctgc cggctataag aatattgtcc ggctcggcct taccgtccgt tttggcaaca
      421 aggtgttcat gaagcgtttt ttcttccata atatcagtcc tttacctcat caaagctgaa
      481 agcacggtta aaaatctcaa tgtttttaac acttgcaccg catttgattt cacctgcggc
      541 aatatctgcg cctgcatttc tcacatcata gccggagcaa tcgagcttac cgtctatata
      601 gagcttaatc ataccgtttc tttcacgcaa ggcgtatacc tttacatcat ctttgtcggc
      661 aattatcgta tcggattttg ccttaattcc cttaacgtcg aaaacaagct tcccgtttgc
      721 gacaaaaatg ctcatatctt tgccgccgag aagtatcata ccgtctgtgg gaacagcaga
      781 aagcgtcagc ctgagagcaa agtcaccgcg ccccgaaacg ccgtaggata taggtataca
      841 cccgtttttg aaatatgtta cgggtacttt ctcggaaaga acgtttccgt aaatatcttt
      901 tacatctatg tctatatcaa gcttttgtaa attttcgccc tcggcaggca aatacactct
      961 gacatcgctg taattagccc gaagctcttt tttaagagcc acgccgccaa gtgtaaaggt
     1021 gctcgctttt acagcgatat gactgttaaa tctgaattca acggtctttt cgtcgatgaa
     1081 ttttgcctct gtaagcttta acggagctgt attttcgttt gtaaacttga aaattcgtat
     1141 ttcatgcggc tccaaatcca ccgaaacggt gtcaccgtat ga
//
LOCUS       DBMDVO010000557         1181 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55647_length_1181_cov_1.703704, whole genome shotgun sequence.
ACCESSION   DBMDVO010000557 DBMDVO010000000
VERSION     DBMDVO010000557.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55647_length_1181_cov_1.703704"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(67..1032)
                     /locus_tag="ACZPE2_07420"
     CDS             complement(67..1032)
                     /locus_tag="ACZPE2_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1319713.1"
                     /translation="MKKFLAVLLSLVMAFSFCLPAFAEDTTASGGGIGDILGGITLPD
                     VDISGAADTIKQLVGIIINSNGKMDEAVIAEIITLIGKIAVSGKIDVETVLKILNALT
                     KGGNSGGSTPNPDAPAGGSVRSALDALTDDQRDYINGLIDIYLTTDFDNNDYKDKDGN
                     DAYNPAKVTALINAMSRMDYADIQKVLDELHSSKNAKGEDINPALSDEAYEVLSAEVA
                     RQEEVSKNNAATAGPTEATTSAIDGVKGFIGKAFETIKGLFGGGNKGGDSPTTTKKST
                     TSKGGSSTTKGKIPQTGDVALFSVAGVAVAAGIALVLTKKKKEDK"
BASE COUNT          300 a          271 c          253 g          357 t
ORIGIN      
        1 acatcaggca aaaaaaatca agcggctttg cctttacaaa gccgcttgta ataattacag
       61 ggttaattat ttatcctctt ttttcttctt tgtgagaaca agagcaatac ccgctgccac
      121 agctacgcct gctaccgaga agagagcaac gtcaccggtc tgcggaatct taccttttgt
      181 ggttgaagag ccgcccttag aagttgttga cttcttggtt gttgtgggtg aatctccgcc
      241 tttgtttccg ccgccgaaga ggcccttaat tgtctcaaaa gctttaccga tgaagccctt
      301 aacgccgtcg atagcacttg tggtagcctc tgtcgggcct gcagttgctg cattgttctt
      361 ggaaacctct tcctgacgag cgacctcggc cgaaagaacc tcataagcct catctgacaa
      421 agcgggattg atgtcttcgc ccttagcatt cttggaagaa tgaagttcat caagaacctt
      481 ctggatatcg gcataatcca ttctgctcat agcattgata agagccgtaa cctttgccgg
      541 gttgtaagca tcgtttccgt ctttatcttt ataatcattg ttatcaaaat cagtagtgag
      601 atatatatca ataagaccgt tgatataatc tctctggtca tcggtaagtg cgtcaagagc
      661 acttcttacg gaaccgcctg ccggagcatc ggggttcgga gtgcttccgc cggaattgcc
      721 gcccttggta agagcgttaa gtattttaag gacagtctca acatcaattt ttccacttac
      781 ggcaatctta ccgataagag taatgatctc tgcgataaca gcctcgtcca tcttgccgtt
      841 gctgttgatg atgatgccga caagctgctt aatggtatcg gcagctccgc ttatatcaac
      901 atcgggaaga gtgataccgc cgagtatgtc gccgattccg ccgccgcttg cagtagtgtc
      961 ttcagcaaaa gccgggaggc agaatgaaaa tgccatcact aaggaaagca acactgctaa
     1021 gaactttttc ataattaaat ccctcttatt tcaattattt gagttgtcac tcattattta
     1081 atgatatttt actatatgtt tttgcggttg tcaactagaa aaagagaata tttttccgta
     1141 attttggtgt ttttttaata attccacact ttgcggcgtc a
//
LOCUS       DBMDVO010000558         1181 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55667_length_1181_cov_1.261111, whole genome shotgun sequence.
ACCESSION   DBMDVO010000558 DBMDVO010000000
VERSION     DBMDVO010000558.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55667_length_1181_cov_1.261111"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            155..280
                     /locus_tag="ACZPE2_07425"
     CDS             155..280
                     /locus_tag="ACZPE2_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319714.1"
                     /translation="MFLFCKSDVADPWYSGDFDKAYNDIEKGCNALLNFFLFENS"
     gene            417..>1181
                     /locus_tag="ACZPE2_07430"
     CDS             417..>1181
                     /locus_tag="ACZPE2_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319715.1"
                     /translation="MDLLKDNVGKLYRKFFIASFGSALIACIYGLVDMAMVGQYHGPD
                     GSAAMAVISPIWNIIYSFGLLCGIGGSVIYAVARGERDDVKEANGYFTSAFILGVLWA
                     LILWVLVWVCEEPLLRLFGANDALMPLCKEYMVAPKLTVPVYVFINLLSSFLRNDGDP
                     GRATKAVIFGGIFNVFGDYFFVFTLDMGIKGAGIATAMGASMSVLVMLTHFISKKNTL
                     KFVKPLRILNSFKMISVNGFSSFIVDVAMGIATMLFN"
BASE COUNT          318 a          215 c          268 g          380 t
ORIGIN      
        1 tgctgcagag catcctccgg gcgatgcagg atattttgaa ataagtttat tcatttacaa
       61 tcacctctta tgtttacatt atataattag attgcaaatt tgtcaatata gtatttaata
      121 attttaaata ctatttttag atgtaaaagg atatttgttt ctattttgca aatccgacgt
      181 tgccgacccg tggtattcgg gcgactttga caaagcatac aatgacatag aaaagggctg
      241 taatgctctt ctcaatttct tcctctttga aaattcttga ctttgattac aaaagttgat
      301 acaatacaat agctcactcg gagggctggt tatcgtatgg aaataacaag ccggataagg
      361 tgcaggctga aatgcccgtt ccatatccgg tcttttttaa tattcaagga gtaggtatgg
      421 atcttttaaa ggataatgtc ggtaagcttt accgtaaatt ttttatagct tcattcggaa
      481 gtgcactgat agcgtgcatt tacggacttg tggatatggc tatggtaggg cagtatcacg
      541 gaccggacgg ctctgccgca atggcggtaa tttccccaat ttggaatatt atatacagct
      601 ttgggctttt gtgcggtata ggcggctcgg tgatttatgc cgtcgcaagg ggagaacgcg
      661 atgacgtaaa agaggcaaac ggatatttta cctctgcatt tatactcgga gttttatggg
      721 cgttgatact ttgggtcttg gtgtgggtct gcgaggaacc gcttctcagg cttttcggcg
      781 caaatgacgc acttatgccg ctttgcaaag aatatatggt agccccaaag cttactgttc
      841 ccgtatatgt atttataaat ctgctctcgt cgtttctccg taatgacggt gaccccggca
      901 gagccacaaa agccgttatt ttcggcggta tattcaatgt gttcggagat tatttctttg
      961 tgttcacatt ggatatggga attaaaggcg cgggaattgc aacagcaatg ggagcttcaa
     1021 tgtccgttct tgtgatgctt acgcatttta tcagcaaaaa gaatacgttg aaattcgtaa
     1081 agcctctaag aatacttaac agctttaaaa tgataagcgt caacggattt tcttcattta
     1141 tagttgacgt ggcaatgggc atagccacaa tgctgtttaa c
//
LOCUS       DBMDVO010000559         1176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_55964_length_1176_cov_1.109767, whole genome shotgun sequence.
ACCESSION   DBMDVO010000559 DBMDVO010000000
VERSION     DBMDVO010000559.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55964_length_1176_cov_1.109767"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="ACZPE2_07435"
     CDS             <1..468
                     /locus_tag="ACZPE2_07435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1319716.1"
                     /translation="DLKVSVKAPVGCPQKMIDDFVLSHEKWINEHIEKQKNRLSLEKT
                     LDEKRVSELRALAAAELPRRVEYYSEKMGLYPASVKITSAATRFGSCSGKNSICFSYR
                     LMLYPPEAVDYVVVHELAHIKEKNHSARFYALVERYMPDYREREKLLKKTPKL"
     gene            complement(494..>1176)
                     /locus_tag="ACZPE2_07440"
     CDS             complement(494..>1176)
                     /locus_tag="ACZPE2_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014560.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR1319717.1"
                     /translation="KKVELLSYTGDAEKNVQRSALISYTNASTEDIDRILSDDTVADK
                     ITYSLVHSSRPRALEAADFTLRFNDVSLSGITHFTRHRMQSIEIPELIRADRNRHIVP
                     ESVKKDPELLLKYETAFKENAAEYERLRTLGKSEESLVYYLLSGNTLDIVTTMNAREL
                     LTFMKLRTCSRAQWEIRAHAVEALELLRKAAPGIFKYYGPSCFISSCPEGRFCCGRQE
                     EMRKTFSM"
BASE COUNT          296 a          259 c          301 g          320 t
ORIGIN      
        1 gatttaaaag taagcgtaaa ggcgcctgtc ggttgcccgc aaaaaatgat agacgacttt
       61 gttttatcgc atgaaaagtg gataaacgag catatagaaa agcaaaagaa ccgtctttct
      121 cttgaaaaaa cgcttgacga aaagagagtg tcggagcttc gcgcacttgc cgccgcggaa
      181 ttgccgcgcc gcgtggaata ttacagcgag aaaatgggac tttacccggc aagcgtaaaa
      241 ataacctccg cagcgacaag attcggttcg tgcagcggga aaaattcaat atgcttttca
      301 tacaggctta tgctttatcc gccggaagcc gttgattacg ttgttgtgca cgagcttgcg
      361 cacattaagg aaaagaacca ttcggcaagg ttttacgccc tcgtggaaag gtatatgccc
      421 gattacaggg agagggaaaa attgctcaaa aaaacaccga agctttgatt tacaaggctt
      481 cggtattctt attttacatt gaaaatgtct tgcgcatttc ttcctgtctg ccgcaacaga
      541 atctgccctc gggacaggat gaaataaagc agctcggacc gtagtatttg aaaatccccg
      601 gagcggcttt tctcaaaagc tcgagtgcct ctaccgcatg cgctctgatt tcccactgag
      661 ctcgtgagca ggtgcgcagc ttcataaatg tcagtaattc gcgagcattc atggttgtga
      721 ctatatcgag cgtgttgccg ctgagaaggt agtacacgag gctttcctca ctcttgccga
      781 gtgtgcgaag tctctcatat tcggcggcgt tttccttgaa tgcggtttcg tatttaagaa
      841 gcagctcggg gtcttttttg accgattcgg ggacgatgtg ccgatttctg tctgctctta
      901 tcagttcggg aatttctata ctctgcattc tgtggcgtgt gaagtgagtt attccggaga
      961 gtgaaacgtc attgaaacga agtgtgaaat ccgccgcctc gagcgctctc ggccttgaag
     1021 agtgcacaag actgtatgtt attttgtcgg caaccgtgtc gtccgataaa atacggtcta
     1081 tatcctcggt tgaagcgttg gtgtaagaga tgagcgcgct tctctgaaca tttttttcgg
     1141 cgtcgcccgt gtatgagaga agctcgactt ttttgc
//
LOCUS       DBMDVO010000560         1175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56013_length_1175_cov_1.477654, whole genome shotgun sequence.
ACCESSION   DBMDVO010000560 DBMDVO010000000
VERSION     DBMDVO010000560.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56013_length_1175_cov_1.477654"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="ACZPE2_07445"
     CDS             complement(<1..879)
                     /locus_tag="ACZPE2_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1319718.1"
                     /translation="MENAVSLFGSFDENIRLIESEFSVTVLNRGSDLKISGEAENVAK
                     ATTAIERLLALINKGEALSDQNVRYVISLVKDGEEDKLMSLSGDCVCITSKGKPVKPK
                     TLGQKKYVEAIRKNTIVLGAGPAGTGKTYLAVAMAVTAFRAKEVNRIILTRPAVEAGE
                     KLGFLPGDLQQKVDPYLRPLYDALFDMLGAEAFARYQERGSIEVAPLAYMRGRTLDDS
                     FIILDEAQNTTREQMKMFLTRLGFNSKMVVTGDVTQIDLPDGKRSGLTDAMRILRNVP
                     DISINTFTEKDVVRHKL"
     gene            complement(979..>1175)
                     /locus_tag="ACZPE2_07450"
     CDS             complement(979..>1175)
                     /locus_tag="ACZPE2_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319719.1"
                     /translation="DFLLEDESCRELLSAKKYTDLCRSELKNCKIISSDIQKNGSRLS
                     GAYKCEKQIGVESEIFVEKN"
BASE COUNT          295 a          292 c          234 g          354 t
ORIGIN      
        1 taatttatgc cttactacgt ccttttcggt gaacgtattt attgaaatat ccggaacatt
       61 tctgagtatt ctcatagcgt cggtaagccc cgaccttttc ccatccggta aatctatttg
      121 ggtaacatcg ccggttacta ccattttaga gttgaagccg agacgggtca aaaacatttt
      181 catctgctcg cgagtggtgt tttgcgcctc gtcaagtatt ataaagctgt cgtcaagagt
      241 tctgccgcgc atatacgcaa gcggtgccac ctcaatagag ccgcgctcct gataacgggc
      301 gaacgcctct gccccgagca tatcgaaaag cgcgtcatac agcggtctta aataagggtc
      361 aaccttttgc tgcaaatcgc ccggcaaaaa tccgagcttt tcgcctgcct caaccgccgg
      421 acgtgtcaag ataatgcgat tgacctcctt tgcgcgaaag gcagtgacag ccatggcgac
      481 ggcaagatat gttttacccg tgccggcagg acccgcaccc aaaactattg tattttttcg
      541 tattgcctcg acatactttt tctgaccgag cgttttcggt tttacgggtt tgcccttaga
      601 tgttatgcag acgcaatcgc ccgataagga cataagcttg tcctcttcgc cgtccttaac
      661 gagagatatg acatatcgca cgttttgatc ggaaagagct tcgcccttat ttatgagggc
      721 taaaaggcgc tctatggcgg ttgtggcctt agccacattt tccgcctcgc cgcttatttt
      781 aaggtcggag ccgcgattta aaaccgtaac cgaaaattca ctttctataa gtcttatatt
      841 ctcgtcaaag ctgccgaata agcttacggc attttccata cggtcaatac tgaggacctg
      901 ctcaaacact gaaaaaccac cttagataat tttctgtcga tatttaatat atagtatcac
      961 aatttttgtg attagtcact aatttttttc aacaaaaatc tcgctctcaa cgcctatttg
     1021 tttttcacat ttgtatgctc cgctgagtct tgaaccgttt ttttgtatat cagaggaaat
     1081 aatcttacaa tttttcaatt cgctcctgca aaggtctgtg tatttttttg ccgacaaaag
     1141 ctcccgacag ctttcatcct ccaacaaaaa atctc
//
LOCUS       DBMDVO010000561         1173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56193_length_1173_cov_0.848881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000561 DBMDVO010000000
VERSION     DBMDVO010000561.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56193_length_1173_cov_0.848881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            18..176
                     /locus_tag="ACZPE2_07455"
     CDS             18..176
                     /locus_tag="ACZPE2_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020940.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HIR1319720.1"
                     /translation="MSANVISATTQKLSDEIYGSMLLGIPNSQFDKATAFLKNFENIK
                     VEEVSADD"
     gene            238..>742
                     /locus_tag="ACZPE2_07460"
     CDS             238..>742
                     /locus_tag="ACZPE2_07460"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1319721.1"
                     /translation="MAIWETFYVTVLSTALSLLIGLPLGVLLVAGEKGGVLPLPKPVL
                     SILNVIINLLRSIPFLILMIMVFPLSRALIGTTVGTTATIVPLVVAAFPFVARLVESS
                     LREVDPNIIEAAQSMGAKPMQIICKVMIPESIPSLLQNVTIALTTILGYSAMSGIIGG
                     GGLGKVAI"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <753..882
                     /locus_tag="ACZPE2_07465"
     CDS             <753..882
                     /locus_tag="ACZPE2_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319722.1"
                     /translation="YGYYRYKYLVMYAAVILLIILVQIFQSVGTRLALKSDKRLKK"
BASE COUNT          309 a          270 c          276 g          308 t
ORIGIN      
        1 ttgccaccga agatggcatc agcgcaaatg taatatccgc gacaacgcag aagctttcgg
       61 acgaaattta cggcagtatg ctgctcggta ttccgaacag tcaatttgac aaggcaaccg
      121 cttttcttaa aaactttgaa aacattaagg ttgaggaggt gagcgcagat gactgacgga
      181 ctttttacgg cggagtcagt agcagagctt ttgaccgcgt ttaaaacgca gttccccatg
      241 gcgatatggg agacatttta cgttacggtg ctgtcaaccg cactctctct tttaatcggg
      301 cttccgctcg gcgttttgct tgtagccggt gaaaaaggcg gcgtacttcc cctgccgaag
      361 ccggtgctgt cgatactcaa tgtcataata aatcttctaa gatccattcc gtttttgata
      421 cttatgataa tggtatttcc cctctcgcgt gcgctgatag gcactaccgt aggaacaaca
      481 gccacaatag tgccgcttgt tgttgccgcg ttcccgtttg ttgcaaggct cgttgagagc
      541 agtctgcgcg aagtagaccc gaatataatt gaggcggcgc agagtatggg tgcaaagccc
      601 atgcagataa tctgcaaggt gatgatcccc gaaagtatac cctctctttt gcagaatgtc
      661 acaatagctc tcactacaat actcggctat tctgcaatga gcggtatcat aggcggcggc
      721 ggactcggta aagtcgcaat tgnnnnnnnn nnttacggct attaccgtta taaatatctt
      781 gttatgtatg ctgcggtaat actgcttata atacttgttc agatattcca gagcgtcggc
      841 acacgcctgg ctttaaagag cgataaaagg ctgaaaaaat aaaatatcga agtttcatca
      901 cttcaattac gacaaaaggc aaccaaaaca ataaatttcg gttgcctttt tactttgcgg
      961 taaaaaaatc gcacgacaag gaattacttc tttgccgtgc gattttcatt tgttatttac
     1021 agccggtggc ttttatcaat acattccgcc gccctgtgca gccatagcgg cagccgcagc
     1081 agcatcagcc ttcggatccg gaatatcagc tacgagagac tctgtggtga gtaccatagc
     1141 ggcaacggac gaagcgttct gaagagcaga acg
//
LOCUS       DBMDVO010000562         1171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56276_length_1171_cov_1.317757, whole genome shotgun sequence.
ACCESSION   DBMDVO010000562 DBMDVO010000000
VERSION     DBMDVO010000562.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56276_length_1171_cov_1.317757"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="ACZPE2_07470"
     CDS             complement(<1..498)
                     /locus_tag="ACZPE2_07470"
                     /inference="COORDINATES: protein motif:HMM:NF020118.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HIR1319723.1"
                     /translation="MNKEFFEAVQMLEKEKGIPAEYLYEKIQNAIIVALRKDYNGKDV
                     VSCEIDPENYEMNVFVRKNVVDEIDDEDTDITVADAQQYKPGAEAGDIIEIMLDTKDF
                     GRIAAQTAKHVIRQGIRDAEKGQIMMEFQSKQQELVTAKVLSVDPITGNATLEIGKAE
                     AILPKG"
     gene            complement(559..1035)
                     /locus_tag="ACZPE2_07475"
     CDS             complement(559..1035)
                     /locus_tag="ACZPE2_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1319724.1"
                     /translation="MAEKLTGTVGTVWNIAQPLAEELGLILWDVRFQKEGAEWYLRIF
                     IDKQDGGIGIEDCVNMSHALDEPLDEADPIDRSYSLQVSSPGIERELTRDFHFTRYLG
                     SRVIVKFIRPVNGKRVYKCTLKDYDAGLITVEFDEGGGMSFEKKEASFVKLDDFDM"
BASE COUNT          273 a          301 c          238 g          359 t
ORIGIN      
        1 acccttcgga agtattgcct ctgcctttcc gatttcaaga gtggcatttc cggtaatcgg
       61 gtctacactg agaaccttag cggtgacaag ctcctgctgc tttgactgga actccatcat
      121 tatctgaccc ttttcggcgt cgcgtatacc ctgacggata acgtgcttcg cggtctgagc
      181 cgctattctg ccgaagtcct tcgtgtcgag cattatctcg ataatgtcgc ctgcctcggc
      241 gccgggctta tactgctgag catcggctac ggttatgtcg gtatcctcgt cgtcgatttc
      301 gtcaaccacg tttttgcgaa caaatacgtt catttcataa ttttcgggat caatctcaca
      361 cgaaactacg tccttgccgt tataatcctt gcggagcgca acgattatgg cgttctgtat
      421 cttctcatag agatactcgg caggaattcc tttttctttt tcaagcatct gcacagcttc
      481 aaaaaattcc ttattcattt taataaccat tcctttcaag gtagtgccgt gttttttcgt
      541 cacggcgatt attgtctttt acatatcaaa gtcgtcaagc tttacaaacg aagcttcctt
      601 cttttcaaag gacattccgc cgccctcatc gaattcaacc gtgataagac ctgcgtcgta
      661 atcctttaat gtgcatttgt aaactctctt gccgtttacg gggcgtatga atttaactat
      721 cacgcggctg ccgagatatc tcgtaaaatg aaaatccctt gtaagttcgc gctcaatgcc
      781 gggcgaagag acctgaagag aatagcttcg gtctattggg tcagcctcgt caagcggctc
      841 gtctaaagcg tggctcatat ttacgcagtc ttcaattcct atgccgccgt cctgtttatc
      901 tatgaatatg cgaagatacc actctgcacc ctctttttgg aagcggacgt cccacaaaat
      961 gagcccgagt tcctcggcaa gcggctgtgc gatattccag accgttccga cggtaccggt
     1021 caatttttct gccattcgat atatcctttc ataaaaaaag tcttaacaaa actaagagag
     1081 cgggtcgccc cactctcaat catacgaata ttcttactgt gttataatac catatatata
     1141 cggtttgcaa gctttttttc aaaggtcgtc g
//
LOCUS       DBMDVO010000563         1169 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56406_length_1169_cov_0.902622, whole genome shotgun sequence.
ACCESSION   DBMDVO010000563 DBMDVO010000000
VERSION     DBMDVO010000563.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56406_length_1169_cov_0.902622"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(75..>1169)
                     /gene="murG"
                     /locus_tag="ACZPE2_07480"
     CDS             complement(75..>1169)
                     /gene="murG"
                     /locus_tag="ACZPE2_07480"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1319725.1"
                     /translation="GGHINPALASAGELRRRHPDADILFIGTKEHMESKLVPAAGFAF
                     KTIEITGFQRKFSAKNIAKNIKTLFLMISSSREVKKIIKEFKPDVVVGFGGYVSGPVV
                     RTACKLGVKTAIHEQNAYPGVTNKALAKTVDKVMLTVKKAEEHLECKNEPVVTGLPVR
                     SEIIEADREFSRAKLGVSPDSIMVLSMGGSLGAKAINENMTALIAERWQNKNLFFMHS
                     TGKYGKWVPERLKELGVDENKASNVVIREYIDDMDVCLAASDLVIGRAGASSLSEIEA
                     VGRASILIPSPNVAENHQYHNAMALVENDAARVIEEKDLTSEKLISEFDSLVADRETL
                     VRLGRNAKKMAVENSSAMICDIIEKLAEKK"
BASE COUNT          274 a          290 c          246 g          359 t
ORIGIN      
        1 atacacaccc cttaaagaaa aaggtaaccc tttttcatat tatgcagggc tgtatgtttt
       61 ggaaacgaca gctgttattt tttctccgct aacttttcta ttatatcaca aatcatcgca
      121 gaagagtttt caaccgccat tttttttgca ttcctgccga gtcttacaag agtctcgcgg
      181 tctgcaacaa gtgagtcaaa ctcggatatg agtttttcgg aggttaaatc cttttcctca
      241 ataacgcgcg cggcatcgtt ttccacaaga gccatggcgt tatggtattg atggttttcg
      301 gcaacattcg gtgagggaat aagtattgac gctctgccga ctgcttcgat ctcggaaagc
      361 gacgacgcac cggctctgcc gataacaagg tcacttgccg caagacagac atccatatca
      421 tctatatatt cgcgtattac cacgttgctt gctttgttct cgtccacgcc gagctctttt
      481 agcctttcag gaacccattt accgtatttt ccggtagaat gcataaagaa aagattttta
      541 ttttgccaac gctcggctat caaggcagtc atattttcat ttatcgcctt tgcacccaga
      601 ctgccgccca tcgagagtac cattatgctg tcgggcgaga caccgagctt tgcacgtgaa
      661 aattccctgt ccgcttctat gatttcgctt cttaccggaa gccccgtaac tacaggctca
      721 tttttacatt caaggtgttc ctctgccttt ttaaccgtga gcataacctt gtccaccgtt
      781 ttggcgagag ccttgtttgt aacgcctgga taggcgtttt gctcatgaat ggctgtcttt
      841 acgccgagct tacaggccgt tctgacaacg ggaccgctga catatccgcc gaagcctact
      901 acaacgtccg gtttaaattc tttgattatt tttttgacct ctctcgatga gcttatcata
      961 agaaagagag ttttaatatt tttagctata tttttagcgg aaaactttct ctggaagccg
     1021 gtaatttcaa tggttttaaa ggcgaacccc gccgcaggta cgagctttga ctccatatgc
     1081 tcctttgtac ctataaaaag tatgtcggca tccggatggc ggcgtctgag ctcgcctgcg
     1141 gacgcaagcg ccggatttat atgtccgcc
//
LOCUS       DBMDVO010000564         1165 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56646_length_1165_cov_1.077068, whole genome shotgun sequence.
ACCESSION   DBMDVO010000564 DBMDVO010000000
VERSION     DBMDVO010000564.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56646_length_1165_cov_1.077068"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            57..1127
                     /gene="hrcA"
                     /locus_tag="ACZPE2_07485"
     CDS             57..1127
                     /gene="hrcA"
                     /locus_tag="ACZPE2_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1319726.1"
                     /translation="MLKRKKASDMEMTERKKKILAAVVERYILSGEPVGSKALVDLPG
                     LSVSSATVRNDMADLVTAGYLEQPHTSAGRIPSSEGYRYYVDNLMGRYELSDKEKRII
                     DSKLEGVPSETKSVLRKAGLVLSELTGCAAVSTTPADSQAVIRRVELVPIGTKTAMVV
                     MLTSSGILKSRVCRTNSEITLDIVETFYNITKQHFIGKPAASLSIAKIQTVALSLGDK
                     SFAMTPLLVTLADLAALTEHTELLLEGQSNLLNYREFESNAYELMEFLRRSEPLSQVF
                     KKYTEKKGTEDTPNVLIGRENLFRELQNSSMIFGEYKVGGRDSGTIGIIGPTRLDYSR
                     LIPSLKYLTDIVSRILSHNIDD"
BASE COUNT          320 a          260 c          288 g          297 t
ORIGIN      
        1 ttgtttcggg gataatatat gtatagttta gcactcagag ataaagagtg ctaacagtgc
       61 taaaaagaaa gaaggcgagc gatatggaaa tgacagagcg aaagaaaaag attcttgccg
      121 ccgttgtaga gagatatata ttgtccggtg aacctgtcgg ctcaaaggct cttgtggatt
      181 tgcccgggct ttccgtttcg tccgctaccg ttcgcaacga tatggccgac cttgtcacgg
      241 caggttatct tgagcagcct cacacttcgg caggcagaat tccgtcaagt gagggctatc
      301 gctattatgt cgataacctt atgggcagat atgagctttc cgataaagaa aaacgcatta
      361 ttgactcaaa gcttgagggc gttccgtccg agacaaagag cgtactcaga aaagccggac
      421 ttgttttatc ggagcttacg ggctgcgctg ctgtttccac aacgcctgcc gacagtcagg
      481 cggttatacg ccgcgtcgag cttgtcccca taggcacaaa aaccgcgatg gtcgttatgc
      541 tgacttcgtc gggtatactc aaaagccgtg tgtgcagaac aaattccgag ataacccttg
      601 atatagtcga gacgttttat aatataacaa aacaacattt tatcggtaag ccggcggcga
      661 gtctcagcat cgcaaaaatc caaacggtgg cactgtcgct cggtgataaa tcgtttgcga
      721 tgactccgct tcttgtaaca cttgcagatt tggcagcttt gacggagcat accgaactgt
      781 tgcttgaagg tcagtcaaat cttctgaact atcgcgagtt tgagtcaaac gcatacgagc
      841 ttatggagtt tctccgccgc tcggagcctt tgtcgcaggt gttcaaaaag tacaccgaga
      901 agaaggggac ggaggataca ccgaatgttt taataggccg cgaaaacctg tttcgcgagc
      961 ttcagaattc aagtatgata ttcggtgaat ataaggtcgg cggcagagac agcggcacta
     1021 tcggaattat aggccccacg aggcttgatt acagccgcct tattccgagc cttaagtatt
     1081 taaccgatat tgtcagcaga atactctcac acaatattga cgattagctt ttaattcggc
     1141 aacgaattta acataacctg ttcat
//
LOCUS       DBMDVO010000565         1160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56981_length_1160_cov_1.056657, whole genome shotgun sequence.
ACCESSION   DBMDVO010000565 DBMDVO010000000
VERSION     DBMDVO010000565.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56981_length_1160_cov_1.056657"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1160)
                     /locus_tag="ACZPE2_07490"
     CDS             complement(<1..>1160)
                     /locus_tag="ACZPE2_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319727.1"
                     /translation="IERLQCSGKEFAEQCGLSEATVSRYKAGSRIPKPGSEDLQKLCR
                     GIHALAEQKGMDIAYQTVLQELTDHAEIPPFDYASLQKKFGQLCAAFSVNLADMAKKL
                     PYDASYLSRIKSGKRKPAAPQKFASDIALYISAYYHTENHKRIAAELIGISPDHLKTP
                     KAYATRLGFWLTNSENAEQEKPSENPVNRFLSSLDAFDLNAYIKSIRFDELKVPSLPF
                     QLPTSKTYYGTEQMKNGELDFLKATALSKSKHSVFMCSDMPMEDMAADLEFSKKYMFG
                     LAAMLKKGLHLNVVHNLNRPFDELMLGLECWIPLYMTGQITPYYLKGTHNSVFGHLLN
                     VSGAAALSGECIAGFHNKGKYELTNSKDELAYYQERSACILKKAHSLMDIYR"
BASE COUNT          259 a          267 c          319 g          315 t
ORIGIN      
        1 tcgcggtaga tatccattaa cgaatgtgcc tttttcaaga tacacgcgct gcgctcttga
       61 taataggcaa gctcatcctt gctgtttgtc agctcgtatt tgcccttgtt gtgaaacccg
      121 gcaatgcatt cgccggataa ggcggcggca cccgatacat ttagcaaatg tccgaaaacg
      181 gaattgtgcg ttcctttcag ataatacggc gtgatttgcc cggtcatata taacggtatc
      241 cagcattcca agcctaacat cagctcgtca aacggacggt tgaggttgtg gacgacgttt
      301 aagtgcaagc cttttttcag catagcggcg agtccgaaca tatatttttt tgaaaactcc
      361 aaatcggcgg ccatgtcctc catcggcata tcgctgcaca taaaaacgct gtgcttggat
      421 ttggaaagcg cggtggcctt gagaaaatca agctcaccgt ttttcatttg ctccgtccca
      481 taataggttt tgcttgtcgg cagctgaaac ggaagtgacg gcacctttaa ttcgtcgaag
      541 cgaatggatt tgatgtaggc gtttaagtcg aaggcgtcga gagaagacag aaagcggttg
      601 acgggatttt cggacggttt ctcttgttcc gcgttttcgc tgtttgtgag ccaaaagcca
      661 agccttgtgg cataggcctt gggcgttttg agatgatcgg gcgatatgcc gatcagttcg
      721 gcagcgattc ttttgtgatt ttcggtgtga tagtaggccg aaatgtaaag cgcgatatcc
      781 gacgcgaact tctgcggcgc ggcaggtttt cgcttgccgc ttttgatgcg ggacagatac
      841 gaggcgtcat acggcagttt tttggccatg tcggcgaggt tgaccgaaaa agcggcacac
      901 agctgtccga atttcttttg cagagacgca tagtcaaacg gcggaatctc cgcatggtcg
      961 gttagctcct gcaaaacggt ttgataggct atgtccatgc ctttttgctc ggcaagggca
     1021 tggatgccgc ggcagagctt ttgtaaatcc tcagagccgg gctttggaat acgcgaaccg
     1081 gctttgtagc ggctgacggt cgcttcggat aagccgcatt gttcggcaaa ctctttgccg
     1141 gagcattgca agcgttcgat
//
LOCUS       DBMDVO010000566         1160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_56984_length_1160_cov_1.018886, whole genome shotgun sequence.
ACCESSION   DBMDVO010000566 DBMDVO010000000
VERSION     DBMDVO010000566.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56984_length_1160_cov_1.018886"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>333)
                     /locus_tag="ACZPE2_07495"
     CDS             complement(<1..>333)
                     /locus_tag="ACZPE2_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319728.1"
                     /translation="AVPVQDENGIPYSPRKQGAGLVQLQNAVKTDVFLLNSDNTKPKA
                     EIGYNESGNFSFDFKAVSIGDDTVQYEPTITVLTEDTVSENGVVYMAQKARKLSDDEV
                     SVTIPKKIT"
     assembly_gap    334..343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<344..>1160)
                     /locus_tag="ACZPE2_07500"
     CDS             complement(<344..>1160)
                     /locus_tag="ACZPE2_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR1319729.1"
                     /translation="AAGNEYSSTYENAAGNDLPKATEPDNGVVASPSTYEAALSIASM
                     NNIETTSIYLLANGRKIRYNDPAEKESDRLISLSGTFEYVDCGIGAATDFSGKNLKEK
                     IALIQRAGEENGEVLTFAQKESNAKNVGAIAAIIYDNVDGALINMSTDNKIPCVFISK
                     ADGEYLCGQPDKKLSVSEDYVDTFKDNYSGKMSYFSSWGVTSDLKLKPEITAPGGDIY
                     STLPNGLYGNMSGTSMASPHMSGAAAVMQQYISENRDGINMTAEQRTSLFNALM"
BASE COUNT          278 a          265 c          235 g          372 t
ORIGIN      
        1 gtgatttttt tcggaattgt tacggaaacc tcatcgtccg aaagctttct tgccttttgc
       61 gccatataaa cgacaccgtt ttccgaaacc gtatcttccg tcaaaacggt tattgttggc
      121 tcatattgaa ccgtatcatc gccaatagat actgctttga aatcaaagct gaaatttccg
      181 ctttcattat atccgatttc tgctttcggt tttgtattat ccgaatttaa aagaaaaaca
      241 tcggttttta ccgcattttg cagctgaacc agacccgcac cttgttttct cggtgaatac
      301 ggaataccgt tttcgtcctg aacgggaact gccnnnnnnn nnntcatcag cgcattaaaa
      361 agactcgttc tttgctcggc tgtcatattt attccgtcac gattttccga aatgtattgc
      421 tgcataaccg ctgccgcacc cgacatatgc ggcgatgcca ttgatgtacc gctcatattc
      481 ccgtaaagtc cgttcggaag cgttgagtaa atatctccgc cgggggcagt gatttcgggt
      541 ttcaatttta agtcggaagt aacaccccaa gaagaaaaat aactcatttt tccgctgtaa
      601 ttatctttga atgtgtcaac ataatcttcg cttactgaaa gttttttatc gggctgtccg
      661 caaagatatt cgccgtcggc tttgctgata aatacgcaag gtattttgtt atcggtggac
      721 atatttataa gcgcaccgtc aacattatca taaatgatgg cggcaatcgc accgacattt
      781 tttgcattgc tttccttttg agcaaatgtc agaacttccc cgttttcctc accggcacgc
      841 tgaatcagag caattttttc tttgaggttt ttgcccgaaa aatctgttgc agcaccgatg
      901 ccgcaatcga catattcaaa tgtgccggaa agagatataa gccggtcgct ttctttttct
      961 gccggatcat tataacgaat ttttctgccg tttgcaagca gatagattga tgtcgtttcg
     1021 atattgttca tactcgcaat cgaaagagcg gcttcatagg ttgatggtga ggcgaccaca
     1081 ccgttatcgg gttctgtcgc tttcggcaaa tcgtttccgg ctgcattctc atatgttgag
     1141 ctgtattcgt ttccggctgc
//
LOCUS       DBMDVO010000567         1159 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57025_length_1159_cov_1.300567, whole genome shotgun sequence.
ACCESSION   DBMDVO010000567 DBMDVO010000000
VERSION     DBMDVO010000567.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57025_length_1159_cov_1.300567"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1159)
                     /locus_tag="ACZPE2_07505"
     CDS             complement(<1..>1159)
                     /locus_tag="ACZPE2_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HIR1319730.1"
                     /translation="HYDLDDSAAHMIRDNNKCILCRRCVAACDAQGISVIGANARGFD
                     THISSAFDRDLATVSCISCGQCIVNCPTGAIVEKDDTGKVLEAINDPDKFVIVNTAPS
                     IRATLGEAFGMKIGTNVEGKMVAALRRLGFDKVFDTDFAADLTIIEEANELVDRVKNG
                     GVLPMITSCSPGWIKYCEHYYPELIPHLSTCKSPQQMFGATMKTWYAKKLNMDPSKMV
                     VVGVMPCTAKKFETKRDGEAASGYPDVDISITTRELARMIESAGIYFKHLPDEEFDNP
                     FGESTGAATIFGATGGVMEAALRTAVKLITGAEAPSPEFNDVRGMKNIKEAQYEVGGL
                     DVKVAVASGTKNAGYLMDKIKDGTGDYTFIEIMGCPGGCINGGGQPIQHAVV"
BASE COUNT          255 a          320 c          277 g          307 t
ORIGIN      
        1 gtacaacggc gtgctgtata ggctgaccgc cgccgtttat gcagccgccg gggcagccca
       61 ttatctcaat aaaggtgtag tcgcccgtac cgtcctttat cttatccata agatagcctg
      121 cgtttttggt gccgcttgca acagcaacct ttacgtcaag accgccgacc tcatactgcg
      181 cctcttttat atttttcatt ccgcgaacat cattaaattc cggcgacgga gcctccgcac
      241 ccgttataag cttaaccgcg gtacgaagag ccgcctccat aactccgccc gttgcgccga
      301 aaatagttgc ggcaccggtg ctctcgccga acggattgtc gaattcctca tcgggcaagt
      361 gtttaaagta aattcccgcg ctctcaatca tacgggcaag ctctcttgtc gtaatcgaga
      421 tgtcaacgtc aggatagccg cttgccgcct cgccgtcacg cttcgtctcg aatttctttg
      481 ccgtgcacgg cataacgccg acgaccacca tcttggaggg gtccatattg agcttctttg
      541 cataccaggt tttcatggtt gcgccgaaca tctgctgtgg tgatttgcag gtggagagat
      601 gcggtataag ctcgggataa tagtgctcgc agtatttaat ccagccgggt gagcaggagg
      661 taatcatcgg aagaactccg ccgtttttaa ctctgtcaac aagctcattg gcctcttcta
      721 taatggtaag atcggctgca aaatcggtgt caaaaacctt gtcgaatccg agtctgcgaa
      781 gagcggcaac cattttaccc tcaacgtttg tgcctatctt cataccgaat gcctcgccga
      841 gagttgccct gattgacggt gcggtgttga cgattacaaa cttatccgga tcgtttatgg
      901 cctcaagcac cttgcccgtg tcgtcctttt cgactattgc gcccgtggga caatttacaa
      961 tacattggcc gcacgatata catgacaccg ttgcgaggtc tctgtcaaaa gccgagctta
     1021 tatgcgtatc aaagcctctc gcgtttgcac cgataacgct tattccctgc gcatcgcagg
     1081 cggcgacgca tctgcggcag agtatgcact tgttgttgtc tcttatcata tgagcggccg
     1141 aatcatctaa atcgtaatg
//
LOCUS       DBMDVO010000568         1159 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57064_length_1159_cov_0.763705, whole genome shotgun sequence.
ACCESSION   DBMDVO010000568 DBMDVO010000000
VERSION     DBMDVO010000568.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57064_length_1159_cov_0.763705"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="ACZPE2_07510"
     CDS             complement(<1..785)
                     /locus_tag="ACZPE2_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319731.1"
                     /translation="MICAACPRACHTDREYDKPSHGFCKMPYNAVIARAALHMWEEPV
                     ISGENGSGAIFFSGCSLRCVFCQNYDISHDGFGKSISEERFIDIMKELVSKGAHNINL
                     VNPTHFVPFIRGALEKYKPPVPVVYNTGGYDRVESIKSLDGLVDIYLPDLKYYDADVS
                     AKYSAAPDYFEKASEAVLEMRRQTETDMFKDGLMQRGLIIRHLVLPGNTAQSVKILKW
                     ISEKLPCDTFVSIMSQYTPCGEAFKYKDLRRKIVSAEYDKVINT"
BASE COUNT          332 a          271 c          251 g          305 t
ORIGIN      
        1 gtgtttatga ccttgtcgta ttcggcggag acgatttttc tcctcaaatc cttatactta
       61 aatgcctcgc cgcacggtgt atattggctc attatgctga caaaggtatc gcacggcagc
      121 ttttcgctta tccatttaag tatttttacg gactgagccg tattgcccgg caatacgaga
      181 tggcggatta taagtcccct ctgcatcaaa ccgtctttaa acatatcggt ttcggtctga
      241 cgtctcatct cgagtacggc ttcggaggcc ttttcaaaat agtcgggagc cgcagaatac
      301 ttcgccgaaa catccgcgtc ataatatttt aagtcaggca aataaatgtc cacgaggccg
      361 tcaagtgatt ttatactctc caccctgtca tagccgccgg tattgtaaac cacgggcacg
      421 ggcggtttat acttttcaag cgcaccgcgg atgaacggca caaaatgagt gggatttacg
      481 agatttatat tatgcgcgcc cttcgacaca agctctttca taatatcaat aaagcgctcc
      541 tcggatatac ttttgccgaa gccatcgtgg cttatgtcgt aattttgaca gaaaacgcat
      601 ctgagtgagc atccggaaaa aaatattgct ccggaaccgt tttcgccgct tataacaggt
      661 tcctcccaca tatgaagcgc cgctctcgct ataacggcat tatacggcat tttacagaag
      721 ccgtgcgagg gcttgtcata ctccctgtct gtatggcagg cacgcggaca ggcggcacat
      781 atcatattgt cacccctttt aataatgtga ataaacagaa gctttaaacg tcgatattac
      841 gaatcatatt tttaacgctg ccgcgaggca gacaattttc cgcataatcg ccgtcacata
      901 ctgccgacag tatggtatca caattcggca caaaaaacaa tgtataagca aacgtccgct
      961 taatcggaca tcggacgaat tattatagag ataagaaagg agttttgacg atgcgtaaaa
     1021 taggcttttg ggcagaaaac gagcaatcca aagagcagaa aaacgaaacg acgcaattca
     1081 aaaccgaaaa aacggaagct aaagaatccg tggtaagagt ttactttccg gcgagaggct
     1141 tcgcttgctc atattataa
//
LOCUS       DBMDVO010000569         1158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57081_length_1158_cov_1.683065, whole genome shotgun sequence.
ACCESSION   DBMDVO010000569 DBMDVO010000000
VERSION     DBMDVO010000569.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57081_length_1158_cov_1.683065"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1158)
                     /locus_tag="ACZPE2_07515"
     CDS             complement(<1..>1158)
                     /locus_tag="ACZPE2_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1319732.1"
                     /translation="GNIYGIDNVKSEIGLPDGLTLKKGELTDGEYTLAVGQSRSNEIT
                     AYIEKASKPGDTTDPVNPGNPETDGSLCPLWLTLSILSGGTLAVLGIKKKCGKRIFSL
                     FLCAVISLTALPTDVFADEVIMPSASGSVTASKEITVNGAKKTITAEVTYNENKPSEE
                     DEVTVTFDAGDGTPVAPITVKKGETISRLPESFGAGKAFMGWFTDTAFTTEFFTNTPV
                     NEDMTVYASFVESANDFQSGVRTTYYVEDCAEYQPITLICDEEITADNVWEYITLFAY
                     TGDTPENFVVTSNEKDEYTLVPETAYTAGCMYRMTAGEGVTFKGLDDTVNEYTFKIYK
                     EQTNIIEVRDNIKYLDKAKLFATDKTDEYMVQADYFEENSLAVGDVLCIDN"
BASE COUNT          276 a          302 c          236 g          344 t
ORIGIN      
        1 cgttatcaat gcaaagcaca tcgcccacag caagagaatt ttcttcaaaa taatccgcct
       61 gaaccatata ctcatcggtt ttatccgtag cgaacagctt ggctttatcg agatacttga
      121 tattgtccct gacctcgatt atattagtct gctctttgta tatcttgaat gtatactcgt
      181 ttaccgtatc gtcaaggccc ttgaacgtta ctccctcgcc tgctgtcata cggtacatac
      241 agccggctgt gtatgcggtt tcgggaacga gtgtatattc atccttctcg tttgatgtta
      301 caacgaaatt ttccggagta tcacccgtat acgcaaataa ggtgatgtac tcccatacat
      361 tatctgccgt tatttcttcg tcacatataa gcgtgatcgg ctgatattcc gcacagtcct
      421 ccacatagta ggtggttctc acacccgatt ggaaatcatt ggcagattcc acaaacgagg
      481 catatacggt catatcctcg tttaccggag tattggtgaa aaattcggtc gtgaatgccg
      541 tatcggtaaa ccagcccata aacgccttgc cagcaccgaa tgattcggga agtctgctta
      601 tcgtttcgcc ctttttaaca gttatcggag ctacaggcgt accgtcaccc gcatcaaagg
      661 tcactgtgac ctcatcttct tccgatggct tattctcgtt atatgtaacc tctgcggtta
      721 ttgtcttttt agcaccgttt acggttatct ccttacttgc cgtaacgctt ccgcttgcac
      781 tcggcattat gacctcgtct gcaaatacat ctgtcggaag cgcagtcagc gagataacag
      841 cgcataagaa cagtgaaaaa atcctttttc cgcacttctt tttaataccg aggaccgcaa
      901 gcgtaccgcc cgataaaata ctcagcgtca gccacagcgg acacagagaa ccgtccgttt
      961 cgggattgcc gggatttaca gggtcggtag tatcgccggg ctttgaggct ttctctatat
     1021 aagccgttat ctcatttgac ctgctctgtc ctaccgcaag tgtatattca ccgtcggtaa
     1081 gctcgccctt cttcagtgta agtccgtccg gcaatccgat ttcagacttt acattatcga
     1141 tcccgtaaat attgccgt
//
LOCUS       DBMDVO010000570         1157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57149_length_1157_cov_1.313447, whole genome shotgun sequence.
ACCESSION   DBMDVO010000570 DBMDVO010000000
VERSION     DBMDVO010000570.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57149_length_1157_cov_1.313447"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="ACZPE2_07520"
     CDS             complement(<1..1087)
                     /locus_tag="ACZPE2_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319733.1"
                     /translation="MAAAVKKEVKIPVLAANLIRSPKQAEEQLEEGIQDFVSLGRTHI
                     ADPHWVNKVKAGKEDEIKRCICCLYCMESMQKNAYKGTHARCSVNPKLGKESEHPIAN
                     GNGRTVVIVGAGPAGLMSAEVLAKRGFKPVVLEKNAFPGGQLQLANKPPLKEKINWCI
                     EDLVTNATHYGAEIKYNVIADESIIKSYNPYAVIIATGGKAIKPKSIKGSDKDNVFTT
                     TDILDGSVVLKNKKVAVIGSGMTGLETSEMLAENGNDITVVEMADSIAPGTWMQHIDD
                     CMPRLKAHGVKFIVGHKLTEITDRGIELNKVDTENILLVDADYVVLSLGVRPENKLYD
                     DINGKLKNVYLIGDAEKTGRIADATSQA"
BASE COUNT          286 a          281 c          224 g          366 t
ORIGIN      
        1 acgcctgaga ggttgcatct gcaattcggc ctgttttttc cgcatcacca atgagataaa
       61 catttttaag tttgccgttt atatcatcgt aaagcttatt ttcgggtctt acgccgagag
      121 acagaacaac atagtccgca tcgaccaaaa gtatattctc cgtatccacc ttattaagct
      181 ctattcctct gtcggtaatt tccgtaagct tatgacctac aataaactta acgccgtgtg
      241 ctttaagtct cggcatacaa tcatcaatgt gttgcatcca agtgccggga gctatcgaat
      301 cagccatttc aaccacggta atatcattgc cgttttcagc caacatttca gatgtttcaa
      361 ggcccgtcat gccggagcct atgactgcaa ccttcttatt ttttagcact accgaaccat
      421 caagaatatc ggttgtcgtg aaaacgttat ctttatcgga gcccttaatt gatttaggct
      481 taattgcttt accgcctgta gctattataa cagcatatgg attgtatgac tttataatac
      541 tttcgtcggc aattacatta tatttaattt cggcaccgta atgtgttgcg tttgtaacca
      601 agtcctcaat gcaccaattg attttttcct taagaggcgg cttattagcg agttgtagtt
      661 gcccaccggg gaaagcgttc ttttcaagaa ctaccggttt gaatcctcgc ttagctaaaa
      721 cttccgcgct cattagaccg gcaggaccgg ctccgacaat tacaacagtt ctgccgttgc
      781 cgttggcaat tggatgctcc gactccttgc ccaatttagg attcacagaa caacgggcat
      841 gtgttccttt atatgcgttt ttctgcatac tttccataca atacaggcag cagatgcatc
      901 ttttgatttc atcctctttg cccgccttaa ccttgtttac ccagtgcgga tcggctatgt
      961 gagttctgcc gaggctgaca aaatcttgaa taccttcctc gagctgttct tccgcttgct
     1021 taggagagcg aattaggtta gcagccaata ccgggatttt tacttccttt tttactgctg
     1081 cggccatata ttttctccag ccgggctcaa aagaggtagg ctccaaccaa taattatatg
     1141 tatcataacc ggcacag
//
LOCUS       DBMDVO010000571         1157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57163_length_1157_cov_1.144886, whole genome shotgun sequence.
ACCESSION   DBMDVO010000571 DBMDVO010000000
VERSION     DBMDVO010000571.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57163_length_1157_cov_1.144886"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="ACZPE2_07525"
     CDS             <1..574
                     /locus_tag="ACZPE2_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1319734.1"
                     /translation="VDSNCQRENLTLMEKARSYRMKADALSHQGKSLGQNVPKSEDNR
                     TTAKIGNEAGDSYKTVQRLIRLTYLVPELQGFVDSGKMKMLPAYELSFLNEEAQRDVI
                     DRIDETESFPSHAQARRMRTAFEEGKLDYDTVTEIINELKPNQVEKLKIPMDDIRKYV
                     PKSMTPAEMQAYLLKLVKQDYDRQHNRDAR"
     gene            584..844
                     /locus_tag="ACZPE2_07530"
     CDS             584..844
                     /locus_tag="ACZPE2_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319735.1"
                     /translation="MNKFIVGFGAAPQEYVVNGVRYVVEPRFQKPDYKEPKENTVLNN
                     RIGNYLTSDFADLPSVGASNIISTEYDCTAAGKEDNNAAEKE"
     gene            825..>1157
                     /locus_tag="ACZPE2_07535"
     CDS             825..>1157
                     /locus_tag="ACZPE2_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1319736.1"
                     /translation="MQPKKNNKIGITALYCRLSRDDGMDGESNSIVNQKTLLSQKAKE
                     KGLTDIKFYVDDGYTGTNFNRPGFGQLISDIEMGYIYAVMVKDLSRLGRDYVSVGNYT
                     DVYFPDHDI"
BASE COUNT          375 a          239 c          272 g          271 t
ORIGIN      
        1 ggtagattcc aattgtcagc gtgagaatct aacgcttatg gaaaaggctc gttcttaccg
       61 tatgaaagca gacgcattat ctcatcaagg caaatctttg ggacagaatg tcccgaagtc
      121 cgaggacaat cgaactacgg caaaaatagg taatgaagcc ggcgacagct ataaaaccgt
      181 tcaacgccta attcgtttaa cctatctcgt tcctgaactt caagggtttg tagatagcgg
      241 caagatgaaa atgctcccgg cttatgaatt gtcttttcta aatgaagaag cacaaagaga
      301 tgtcattgac cgcattgatg aaacggaaag ctttccatcc cacgcacagg ctcgccgtat
      361 gcgcaccgct tttgaagaag gcaagcttga ctatgatacc gtaaccgaaa ttataaacga
      421 gctgaaaccg aaccaagtgg aaaagctcaa aatcccaatg gacgatatac gcaagtatgt
      481 gccgaaatcc atgacacccg ctgaaatgca ggcatacctt ctcaagctcg taaaacagga
      541 ttacgaccgt cagcataacc gcgatgcgag ataaggagag cttatgaata agtttattgt
      601 aggcttcggc gccgcaccgc aggagtatgt tgttaacgga gtaagatatg tggttgagcc
      661 gagatttcaa aagcccgact acaaagaacc taaagagaat actgtgctga ataaccgtat
      721 cggaaattat ctcacaagtg atttcgcaga tttgccgtct gtcggtgcaa gtaatataat
      781 aagcaccgaa tatgattgta cggctgccgg gaaggaggat aataatgcag ccgaaaaaga
      841 ataacaaaat cgggataacg gcgctttact gccgtttgtc ccgtgatgac ggaatggacg
      901 gtgaaagcaa ttcaatcgtc aatcagaaaa cgctgctttc gcaaaaggca aaggaaaaag
      961 gattgaccga tataaaattc tatgtggatg acggatacac aggaacaaat ttcaaccgac
     1021 cgggattcgg gcagttaata tcggatattg aaatgggata tatctacgcc gtgatggtaa
     1081 aagacctatc ccgtctcgga cgagattatg tttcggtcgg taattatacc gatgtgtatt
     1141 ttccagatca cgacatt
//
LOCUS       DBMDVO010000572         1156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57227_length_1156_cov_1.203791, whole genome shotgun sequence.
ACCESSION   DBMDVO010000572 DBMDVO010000000
VERSION     DBMDVO010000572.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57227_length_1156_cov_1.203791"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1156)
                     /locus_tag="ACZPE2_07540"
     CDS             complement(<1..>1156)
                     /locus_tag="ACZPE2_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like domain-containing protein"
                     /protein_id="HIR1319737.1"
                     /translation="GNLLITLSNGTSLNLGNIKGAKGDQGIQGEKGDKGDQGIQGEKG
                     DKGDQGEKGLNGADGKDGTNGTDGVGVASSEINSLGELVIKYSNGTTVNLGRVVGNDG
                     AQGVQGEKGDKGDQGIQGEKGEKGEKGDKGDAGINGTNGADGVGVASSEINALGELVI
                     KYSNGTTVNLGKVVGNDGAQGVQGEKGDKGDKGDQGIQGEKGDKGDQGVQGEKGDKGD
                     AGINGADGINGTNGADGVGITDVTINADDELVLSFSNGNVINLGNIKGSKGDKGDKGD
                     SGLNGKDGVGIQNVDVTADGALTVTLTNGTVLNLGNIKGADGLGITKAEINTSGELVL
                     TYSNGDVKNLGKVSGENGKDGANGADGLGIKSLTLSPDGELVVTMSDNSKI"
BASE COUNT          187 a          352 c          247 g          370 t
ORIGIN      
        1 gatcttggaa ttatcggaca ttgtaaccac aagctcgccg tccggggaga gtgtgagcga
       61 cttaataccg agtccgtcgg cgccgttcgc accgtcctta ccgttctcac cgctaacctt
      121 gccgagattt ttgacgtcgc cgtttgaata ggtcaaaaca agctcaccgg aggtattgat
      181 ttccgccttg gttatgccta agccgtcggc acccttgatg ttaccgaggt taagaaccgt
      241 gccgtttgta agcgttacgg tcaatgcgcc gtcagccgtc acatcaacat tctgtatacc
      301 cacgccgtct tttccgttaa gtccgctgtc gcctttatct cccttgtcgc cctttgagcc
      361 tttgatattg ccgaggttta taacattgcc gtttgagaac gaaagcacaa gctcgtcatc
      421 ggcatttatt gtaacatctg taatgcctac accgtcagct ccgtttgtac cgttgatacc
      481 gtcagcaccg ttgatacctg cgtcgccctt atcgcctttt tctccttgaa cgccttggtc
      541 gcccttgtcg cctttttcgc cctgaatgcc ttggtcgccc ttgtctcctt tatcgccttt
      601 ttcgccttgt actccctgtg cgccgtcgtt gccgactacc ttgccgagat ttaccgttgt
      661 tccgttgctg tattttataa caagctcacc gagagcattt atttcgctgc ttgcaacgcc
      721 tacgccgtca gctccgtttg taccgttgat acctgcgtcg cccttgtctc ctttttcgcc
      781 cttttcgcct ttttcgccct gaatgccttg gtcacctttg tcaccttttt cgccttgaac
      841 tccctgtgcg ccgtcgttgc caaccactct gccgagattc acagttgttc cgttgctgta
      901 ttttataaca agctcaccga gagaatttat ttcgctgctt gcaacgccta cgccgtcggt
      961 gccgttcgtg ccgtctttac cgtctgcacc gttgaggcct ttttcgcctt ggtcgccctt
     1021 gtcacccttt tcgccctgaa tgccttggtc gcccttgtca cccttttcgc cttgaatgcc
     1081 ttggtcgccc tttgcgccct ttatgtttcc gagattaagt gacgttccgt tcgaaagagt
     1141 aattaaaaga tttccg
//
LOCUS       DBMDVO010000573         1155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57278_length_1155_cov_1.890892, whole genome shotgun sequence.
ACCESSION   DBMDVO010000573 DBMDVO010000000
VERSION     DBMDVO010000573.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57278_length_1155_cov_1.890892"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZPE2_07545"
     CDS             complement(<1..476)
                     /locus_tag="ACZPE2_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319738.1"
                     /translation="MQNSSASSAAGRLNSSLRTVASVPSGEISDKDEFLKGIWVDENG
                     FVASFSENGRTAVFDEQTDEQTRKSVKITAQDKDTISISCEDKNYTAYHADSEKGAEL
                     CENLLTAASGDWSMCEYGYAQTVEIRKFTFLTSLGNDFSGDKITVGLEGVKLRNSL"
BASE COUNT          264 a          257 c          273 g          361 t
ORIGIN      
        1 aataacgagt tgcgtagctt tacaccctca agtcctacgg taatcttatc gcctgagaaa
       61 tcgtttccga gcgatgtcag aaaggtgaat tttctgattt ctaccgtttg cgcatagccg
      121 tattcgcaca ttgaccaatc tcccgaagcg gcggtcagga gattttcgca aagctctgcg
      181 cctttctcgc tgtcggcgtg gtatgcggta taattcttat cctcgcagga aatgcttatc
      241 gtatctttgt cctgtgccgt aattttaacg gattttcggg tctgctcgtc tgtctgctcg
      301 tcaaataccg ctgtcctgcc gttttctgaa aagctcgcca caaagccgtt ttcatcgacc
      361 catatacctt tcagaaattc gtctttatcc gatatttcgc ctgacggcac agatgccacg
      421 gtgcgcagtg agctgtttaa tctgcctgcg gctgatgacg ctgagctgtt ttgcaaagag
      481 gcgtctgaat ttgctctgcc tgctgtgcag gaggacagta atatcgctgt gattaatgct
      541 gtagctgtta ttgaaaataa aaaccgattc tttctcatat tgattccttt ctgatatttg
      601 tctgatattt acagtaatgc agtgtcttga tttcattaac cgccgtgaag cttttctgta
      661 cataatttta ccataaaatt atgattatta caataggaca agcaacataa aaacgaaatt
      721 acaggcaatc gaaaaatctc tatcgttgat aaaaatgggg atgtcgagtg ttgcttgctc
      781 tgttggtttt gcatctctgc aaagtcagct gtttgtgccg ttgcatctgt gcattgacag
      841 ccatttgtgc cgttgcatcg tgcaacggct ttgtgtggct gtctttatgc atccttgcat
      901 aacggctttg tgtggctgtc attatgcgtc cttgcatagt ggctttgtgt ggctgactgc
      961 aagcatcctt gcttggactt ggggcggtag ccccacgctc agccccggca tggatgccgg
     1021 gcgtgtatgc caaagggctt tgcaaggacg caaagccaac caagcatata cagcctttca
     1081 cgggggaaag gggtttgggg atagggattg aggataagca cttttttgaa aaacatactt
     1141 tttctacaag ctgac
//
LOCUS       DBMDVO010000574         1155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57287_length_1155_cov_1.525617, whole genome shotgun sequence.
ACCESSION   DBMDVO010000574 DBMDVO010000000
VERSION     DBMDVO010000574.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57287_length_1155_cov_1.525617"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="ACZPE2_07550"
     CDS             <1..759
                     /locus_tag="ACZPE2_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319739.1"
                     /translation="MKYFELSGLFEKRKIAVVDSGWMGTVQKTLTALVNGRTDENIIG
                     YYFGLYRREDDNYKAFLFDVTDAYKYVPTFCNNLFECFCSAPHGMTTGYRECDGKIIP
                     VLSDANAEMVKMAEIQTKTAVRFTEYACENENYIPICKMRDISAQLLKALMYKPDKQE
                     AEILGAFPFSDDVTEAKIQHLAGNCKGSISNILLPVRIIRKKKGKSIYPDKYIYWLYG
                     SIVLTGCKPAWVYRLSVREWERLRLLRERRKLCR"
     gene            750..>1155
                     /locus_tag="ACZPE2_07555"
     CDS             750..>1155
                     /locus_tag="ACZPE2_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1319740.1"
                     /translation="MPLISVIMGVHNSETDMLLSAVGSVLSQSVSDIELIICDDGSEN
                     GIGKTLDSIKDSRVKLISFKENKGLAAALNACIDVASGKYIARQDDDDISLPGRFEKQ
                     TEYLENHSDIDFIGTNCELYSSGEGVYGERIMP"
BASE COUNT          361 a          207 c          301 g          286 t
ORIGIN      
        1 atgaaatatt ttgagctaag cggtttattc gaaaaaagaa aaatcgccgt tgttgacagc
       61 ggctggatgg gtactgtgca gaaaacgctg acagcgcttg taaacggcag aacggacgaa
      121 aatattatcg gatattattt tgggctttac agacgtgagg acgataatta caaggcgttc
      181 ctgtttgatg tgaccgatgc gtataaatat gtgccgacct tctgtaataa tctgttcgag
      241 tgcttttgca gtgcgccgca cggtatgact acagggtaca gggaatgcga cggaaaaatc
      301 attcccgtgc tgtccgatgc aaacgcagaa atggtaaaaa tggctgaaat acagacgaaa
      361 acagctgtaa gatttacgga atatgcgtgt gaaaatgaaa attatatccc gatatgcaaa
      421 atgagagata tatccgccca acttctaaag gctcttatgt acaagcccga taaacaggag
      481 gcggaaattc tgggagcgtt cccgttcagc gatgatgtga ccgaagcgaa gattcagcac
      541 cttgcgggaa actgtaaagg cagtatttca aatattcttc tgcctgtcag aattatcaga
      601 aagaaaaaag gaaaaagcat ttatcccgat aaatatattt actggctgta cggcagtatt
      661 gtactgacag ggtgtaagcc tgcatgggtt tacaggctca gtgtgcggga gtgggaaagg
      721 ctaagactgc tcagggagag aagaaaatta tgccgttaat atcagtaata atgggagtgc
      781 ataacagcga aaccgatatg ctcttaagtg ctgtcggctc ggttttgtcg caatcggtat
      841 ccgatattga gcttataata tgtgatgacg ggtctgaaaa cggcataggt aaaacgcttg
      901 acagtattaa agacagccgt gtgaagctga tttccttcaa ggaaaacaaa ggacttgcgg
      961 cggctctgaa tgcctgtatt gatgttgcaa gcggaaaata tatcgcaagg caggacgatg
     1021 acgacatcag ccttccggga aggtttgaaa aacagaccga gtaccttgaa aatcacagtg
     1081 atattgattt catcggaaca aattgcgagc tttattcgtc gggagagggc gtatacggcg
     1141 agcgtataat gccta
//
LOCUS       DBMDVO010000575         1155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57297_length_1155_cov_1.249526, whole genome shotgun sequence.
ACCESSION   DBMDVO010000575 DBMDVO010000000
VERSION     DBMDVO010000575.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57297_length_1155_cov_1.249526"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="ACZPE2_07560"
     CDS             complement(<1..826)
                     /locus_tag="ACZPE2_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR1319741.1"
                     /translation="MKITVIGGGGVRSMFLAKSIAQKAEKLRIDELCFMDNNKEKLRI
                     YGGMASHVANLLCPKMKFMLTTDSIEAVKDADYVITTIRVGEDEMRAKDERIALNHGV
                     LGQETTGASGLSFAMRSVPALAEYCELIKKFSKPNVKVFNFTNPAGVVSQTLRDMGYD
                     FTYGICDAPSGMLRTFALMYGESPNDITGECYGLNHLSYFKSIKLKGREIMPELIEND
                     EAYKKSDMRYFEKELLRDRGCVLNEYLYYFYYREKAVENILKADQTRGEQIRDINKS"
BASE COUNT          308 a          239 c          213 g          395 t
ORIGIN      
        1 tggatttatt tatatctcgt atttgttcgc cacgggtttg gtcggctttg agtatgtttt
       61 caacagcttt ttcacgatag taaaaataat agagatattc gtttaaaacg cagcctctgt
      121 ctcttagaag ctccttttcg aaataacgca tatcactctt tttgtatgcc tcgtcatttt
      181 cgatcagttc cggcattatt tctctgccct ttagctttat actcttgaaa tatgacaggt
      241 gattaaggcc gtaacattcg cccgttatgt cattgggact ttcgccgtac atgagtgcaa
      301 atgtgcgaag cattcccgat ggagcgtcgc atattccgta tgtaaaatca tatcccatat
      361 cacgcagggt ttggctcact acaccggcag gatttgtaaa attaaaaact tttacattgg
      421 gttttgaaaa ttttttgata agctcgcaat attcggcaag agccggtacg ctcctcattg
      481 caaaagaaag accgctcgct cccgttgttt cttgaccgag gacaccgtga ttgagtgcga
      541 ttctttcgtc ctttgctctc atttcatctt cgcccacacg gattgtggtt ataacatagt
      601 cggcatcctt tactgcctca atgctgtccg tcgtcagcat aaatttcatt ttaggacaaa
      661 ggagatttgc aacatgactc gccataccgc cgtaaattct taatttttct ttgttgttat
      721 ccataaagca aagctcgtct atccgcagct tttctgcttt ttgtgctatg ctttttgcga
      781 ggaacattga gcggactccg ccccctccga tgactgttat tttcatatac atatacctcc
      841 ggctgctgtt ctatacacat aattatagta ttgttgtgcc accaataaaa gaatataata
      901 cgacaataaa ttaaatattg tgtcgtatta tataaagttt ttattgaaat aaatttcgat
      961 ttgtattata ttgtttgtat aaacagagag gactttaata tgattttatc atttaattat
     1021 aacggctgcc gaattcagct tttcaaaata ttttcagata cgctcgattc cgtggttccg
     1081 atgcattctc acgcaaaaaa cgggtacgag attcattata ttgccggagg caaaggtgta
     1141 ctcgaaacag aaaaa
//
LOCUS       DBMDVO010000576         1154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57360_length_1154_cov_1.226021, whole genome shotgun sequence.
ACCESSION   DBMDVO010000576 DBMDVO010000000
VERSION     DBMDVO010000576.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57360_length_1154_cov_1.226021"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="ACZPE2_07565"
     CDS             complement(<1..440)
                     /locus_tag="ACZPE2_07565"
                     /inference="COORDINATES: protein motif:HMM:NF021093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1319742.1"
                     /translation="MKSKTEKLIRRFEISLAAAFVITSIFSFVSFAASCGDVRRDVLR
                     MHVIANSDSDEDQILKLKVRDAVLNEGKEIFDGSLTSANAEKLLIPQKERLEETAREV
                     IRKNGFDYDVRLEIGKDFFSTRTYDDRITLPAGEYEAVRVIIGEG"
     gene            complement(542..>1154)
                     /locus_tag="ACZPE2_07570"
     CDS             complement(542..>1154)
                     /locus_tag="ACZPE2_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1319743.1"
                     /translation="LTSSCCPAFVDYVKKFYPKLTENISHNLSPMAAIAKYIKETDET
                     AKVIFIGPCTAKKAEAKQERVAQYVDCVLTFEELQALIDSRDIELTELPEDVLDNASY
                     YGRIFARSGGVSEAVGQAIKEQNIDFTVNPLPCDGIEECKTALLKASRGILKNNFIEG
                     MACVGGCIGGAGCLTHEAKDKNEIDKYGKEALEKSIGDALSQL"
BASE COUNT          287 a          297 c          235 g          335 t
ORIGIN      
        1 ccctctccta ttatgacgcg cacggcctca tattcaccgg caggcaaagt tatacgatca
       61 tcataagttc gcgtcgaaaa aaagtccttg cctatttcga gccttacatc ataatcgaag
      121 ccgtttttac gtattacctc gcgcgccgtc tcttcaagcc gctccttttg cggaataagc
      181 agcttttcgg cattcgcact tgtcaaagaa ccgtcaaata tctccttgcc ctcattgagc
      241 acggcatcgc gcaccttaag cttcagtatt tggtcctcgt cgctgtcgga attagctatt
      301 acatgcatac gcaaaacgtc gcgtcttacg tcgccgcacg acgcggcaaa tgacacaaag
      361 ctgaaaatcg acgttatgac aaatgctgcg gctaacgata tttcaaatct tcgaataagc
      421 ttttctgttt ttgatttcat atgtttttca tcctttcgta aggtaattat tggaagaaaa
      481 atcatattta tacaaaaaaa tgaacgcagg ggtttttccc tgcgttcctg aagctcgatg
      541 tttaaagctg actcaatgcg tcgcctatgc tcttctcaag agcttcttta ccgtatttgt
      601 ctatctcgtt tttatctttt gcctcatggg taaggcagcc tgcgccgcct atacatccgc
      661 cgacacacgc cataccctca ataaagttat ttttaaggat tccgcgcgac gctttcaaaa
      721 gagccgtttt gcactcttct atgccgtcgc acggaagcgg attaacggtg aaatctatat
      781 tctgctcctt aattgcctgg ccgacagcct ccgaaacgcc gccgcttcgt gcaaatattc
      841 tgccgtaata ggaagcattg tcaagaacat cctcaggaag ctcggtaagc tctatatcac
      901 gcgagtcaat aagagcctga agctcctcaa aggtgagaac gcagtcgaca tactgtgcga
      961 ctctttcctg cttcgcctcg gctttttttg ccgtacaagg gcctataaat ataacctttg
     1021 cggtctcgtc ggtctctttt atatattttg caatcgccgc catgggagac agattgtgtg
     1081 aaatgttctc ggtaagcttc ggatagaact tctttacata gtcaacaaac gccggacagc
     1141 aagagcttgt gagg
//
LOCUS       DBMDVO010000577         1154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57363_length_1154_cov_1.164292, whole genome shotgun sequence.
ACCESSION   DBMDVO010000577 DBMDVO010000000
VERSION     DBMDVO010000577.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57363_length_1154_cov_1.164292"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1154
                     /locus_tag="ACZPE2_07575"
     CDS             <1..>1154
                     /locus_tag="ACZPE2_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013400.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH39 family glycosyl hydrolase"
                     /protein_id="HIR1319744.1"
                     /translation="MTELNIDLTKTAGKIKPMNAVNNGPVGGEARDSGNFMLYKEAEI
                     PYARNHDAAFFSDYGGEFTVDVHNIFRNFDADENDPSSYIFEPTDKYIKMTAAAGTET
                     FYRLGSKIEHGFKYGTYPPKDFLKWAKICEHIIRHYNEGWADGFDYNITYWEIWNEPE
                     ATTFDGSHCCWQGTEEQFIEFYEVTAKYLKKNFPNLKIGGPAFCSPWRNNFKRNFLKA
                     VKERDIPLDFYSYHCYANAPIGVCNAVLEGERALSEYGLEDTFTILSEWNYIKGWSGE
                     DWRYSLIREKSIKGAAFIAGSMSVCQNTSLGMLMYYDARPCGMNGLFKDYTYEPLKGY
                     YSFLMFRDLRRLGVHIPTERCVDDIYSCAATDGRNSAIMLTHFNDEESTP"
BASE COUNT          335 a          222 c          286 g          311 t
ORIGIN      
        1 tatgacagag cttaatattg atttaacaaa aaccgcagga aagataaagc cgatgaatgc
       61 cgtgaataat ggtcctgtcg gaggagaagc acgtgacagc ggcaatttta tgttgtataa
      121 agaagccgaa atcccttacg caaggaacca tgacgccgca tttttcagtg attacggcgg
      181 tgaatttacg gttgatgttc acaatatttt ccgcaatttt gatgccgatg aaaacgatcc
      241 ttcatcatat atatttgaac ctacggataa atatattaag atgaccgcgg ctgccggaac
      301 ggagaccttt tatcgtctgg gatcgaaaat tgagcacggt tttaaatacg gaacttaccc
      361 gccgaaggac ttcctgaaat gggcgaaaat ttgcgaacat attatccgtc attacaacga
      421 aggatgggca gacggatttg attataacat aacatattgg gaaatctgga atgagcctga
      481 agccacaacg ttcgacggat cgcattgttg ctggcagggc acggaagaac agtttattga
      541 gttttatgag gttacggcaa aatatctgaa aaagaatttt ccgaacctga aaatcggcgg
      601 tccggcattt tgctctccgt ggagaaataa ctttaagcgc aatttcctta aagccgtaaa
      661 ggaaagagac attcctcttg acttttattc ataccactgc tatgcaaacg ctccgatcgg
      721 agtctgcaat gcggtgcttg agggtgaaag agctctttcg gagtacggcc ttgaagatac
      781 atttacgata cttagtgaat ggaattacat taaaggctgg tcgggcgagg attggcggta
      841 ttcgcttatc cgggaaaaga gcattaaagg tgctgcattt attgccggga gtatgagcgt
      901 ttgtcagaat acaagtcttg gaatgcttat gtattatgat gctcgtcctt gcggaatgaa
      961 cggcttgttt aaggattata catatgagcc gctcaagggg tattattcct ttttgatgtt
     1021 ccgcgaccta agaaggcttg gtgtgcatat tccgacggaa cggtgtgttg acgacattta
     1081 cagctgtgcc gcaaccgacg ggcgtaacag cgcaataatg cttacccact ttaatgatga
     1141 ggaaagcacc cctg
//
LOCUS       DBMDVO010000578         1153 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57452_length_1153_cov_0.940114, whole genome shotgun sequence.
ACCESSION   DBMDVO010000578 DBMDVO010000000
VERSION     DBMDVO010000578.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57452_length_1153_cov_0.940114"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(53..607)
                     /gene="frr"
                     /locus_tag="ACZPE2_07580"
     CDS             complement(53..607)
                     /gene="frr"
                     /locus_tag="ACZPE2_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1319745.1"
                     /translation="MQEVYDFANEKMTKSVKSLHNEYVSMRAGKASVSLLDKVVVDYY
                     GCPTPVQQMAAVSVSEGRNLVIQPWDVSTINAIEKAIQASDLGVNPMNDGKVIRLNFP
                     PLTEEKRKLLAKEVGKYAEEAKVAVRSIRRDAMEKLKKLNKDKAITEDELHDGEDEVQ
                     KITDKYVKEIDDMAKKKEADIMEI"
     gene            complement(633..>1153)
                     /locus_tag="ACZPE2_07585"
     CDS             complement(633..>1153)
                     /locus_tag="ACZPE2_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR1319746.1"
                     /translation="GNMDRTRADHIGMLATVMNALALADTLEQLDVPVRVQTAIEMPR
                     VAEPYIRNRAVRHLEKGRVVIFGCGTGNPFFSTDTAAALRAAEINADVIFKATNVDGV
                     YDKDPNRFADAVKYDELTHTEVLAKGLKVMDSTAASLCRDNNIPILVFNLNDPENIIR
                     AAAGEKIGTLVK"
BASE COUNT          262 a          285 c          277 g          329 t
ORIGIN      
        1 gcataccgca aggcgcaaga aacgccgtat tgcggaaaaa cagcccgaaa aatcagattt
       61 ccataatgtc ggcttccttt ttctttgcca tatcgtcaat ttctttaacg tatttgtcgg
      121 ttatcttctg aacttcgtcc tcaccgtcgt ggagctcgtc ttcggttata gctttatctt
      181 tgttgagctt ttttaatttt tccatagcgt cacggcgaat ggagcgaacg gcaacctttg
      241 cctcctcggc atatttgcct acctctttgg caaggagctt tctcttttcc tcggtgagag
      301 gcgggaagtt aaggcggata accttgccgt cattcatcgg attaactccg aggtctgacg
      361 cctgtatagc cttttcaatg gcatttatag tgcttacgtc ccacggctga atgacaagat
      421 ttctgccctc ggaaaccgag acggcagcca tctgctgaac gggagtgggg caaccgtaat
      481 aatctacgac taccttatcg agcagcgaaa cggaagcttt tccggcgcgc atcgaaacat
      541 attcgttatg aagagactta acgctcttcg tcattttttc attggcaaaa tcataaacct
      601 cttgcatggt ttttcctcct gaaaatatat tgttatttga ctaacgtgcc tattttttca
      661 cccgctgcgg cgcgaatgat gttttcggga tcgttgagat tgaaaacaag tatcggaata
      721 ttgttgtcgc ggcaaagcga agccgcggtg ctgtccatta ccttgagtcc ctttgcgaga
      781 acctcggtgt gagtgagctc atcatatttt acggcgtcgg caaagcggtt ggggtcctta
      841 tcgtaaacgc cgtcaacatt tgtcgcctta aatataacgt cggcgtttat ctcagcagcg
      901 cgaagagccg ccgcagtatc ggttgagaag aacgggttgc cggttccgca gccgaatatt
      961 acaactctgc ccttttcaag gtgacgaacc gctctgttgc ggatataagg ctctgccaca
     1021 cggggcattt cgatggccgt ctgaactctg accggaacgt cgagctgctc aagagtgtcg
     1081 gcaagagcaa gcgcattcat aaccgttgcg agcataccta tatggtcggc tctggttctg
     1141 tccatattac cgc
//
LOCUS       DBMDVO010000579         1152 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57503_length_1152_cov_1.012369, whole genome shotgun sequence.
ACCESSION   DBMDVO010000579 DBMDVO010000000
VERSION     DBMDVO010000579.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57503_length_1152_cov_1.012369"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="ACZPE2_07590"
     CDS             <1..460
                     /locus_tag="ACZPE2_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1319747.1"
                     /translation="REASKFSGAVGVDSDDLFQEGMIGLLSAARSYKNDCGASFRTYA
                     SVCIRNRIISAVKKASGGKSVRRDLLVPLETELLSVPELSYDDGLVVKEECDRLFGFI
                     ETQLSERERSILKLFLSGLSYGEIAQKLGSSSKSVDNALQRVRRKLKKYN"
     gene            570..851
                     /locus_tag="ACZPE2_07595"
     CDS             570..851
                     /locus_tag="ACZPE2_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR1319748.1"
                     /translation="MYEDKTLICKECGKEFVFTAGEQEFYAEKGFVNEPQRCKACRDA
                     RKNAAKGDRQMYTATCARCGGEAKVPFQPKDDRPVYCSECFAKMKEEQA"
     gene            965..>1152
                     /locus_tag="ACZPE2_07600"
     CDS             965..>1152
                     /locus_tag="ACZPE2_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319749.1"
                     /translation="MKKTVSVLLVAIMLCTFSFCAFAVDNTASVNIVETVSNEYTVYL
                     GFIDNGNEVCGGSMNLVY"
BASE COUNT          323 a          200 c          274 g          355 t
ORIGIN      
        1 tcgtgaggct tctaagtttt ccggagctgt cggcgtcgat tccgacgatc tttttcagga
       61 gggtatgata gggctcttgt ctgccgcgcg ttcctataaa aatgattgtg gcgcgtcatt
      121 cagaacttat gcctcggttt gcatacgtaa cagaatcatt tcggctgtaa aaaaagcgtc
      181 aggcggcaaa agtgtgcgtc gtgacctttt ggttcctttg gaaacagagc ttttatctgt
      241 gccggagctt tcttatgatg acggtcttgt tgttaaagaa gaatgtgaca ggctttttgg
      301 ctttatcgaa acacagcttt ccgaaagaga acgcagcatt ttaaaattat tccttagcgg
      361 gttaagctat ggcgaaatag cccaaaaact cggttcttca tccaaatccg ttgacaatgc
      421 gcttcaaagg gttaggcgaa agctgaagaa gtataactaa ggcggtaatt aatacttcat
      481 caaaagccct tttctcatta ggagagcatt tttgcggcgg tgcaattccg cttagggtaa
      541 aaataatttt tttcaagcga ggtagcaaaa tgtacgaaga caaaaccctt atttgtaagg
      601 aatgcggtaa agagttcgtt tttacagcag gtgagcagga gttctatgct gaaaaaggct
      661 ttgtaaacga gccccagaga tgcaaggctt gccgcgatgc aagaaagaac gctgctaaag
      721 gcgatagaca gatgtacaca gcaacttgtg cacgttgtgg cggcgaggca aaggttcctt
      781 tccagcccaa agatgatcgt cctgtttatt gtagcgagtg ctttgcaaag atgaaagaag
      841 agcaggcata attttaacat aaattaaata tatatgtttc gctgagtctg ttcgttaatt
      901 cgggcagact tcttcttttt gtattttttt gaaaaattta tgctattata aatgtggtga
      961 tataatgaaa aagactgttt ctgttttgct tgttgcaatt atgctttgta cattttcttt
     1021 ttgtgccttt gcggtggata ataccgcatc cgtaaatata gttgaaaccg taagtaacga
     1081 atatacggtt tatcttggat ttatcgataa cggtaacgag gtatgcggcg gcagtatgaa
     1141 tttagtatat aa
//
LOCUS       DBMDVO010000580         1149 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57710_length_1149_cov_0.733779, whole genome shotgun sequence.
ACCESSION   DBMDVO010000580 DBMDVO010000000
VERSION     DBMDVO010000580.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57710_length_1149_cov_0.733779"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ACZPE2_07605"
     CDS             complement(<1..543)
                     /locus_tag="ACZPE2_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1319750.1"
                     /translation="MQAHETTYEKLLENVKKYMPDTALVERAYKYAEEAHRSQKRNSG
                     ESYIVHPVGVAIILSELEMDSECIAAGLLHDVIEDVEKDNYNTVKNEFGQVIADLVEG
                     VTKLDKIPFVSNEQRQIENVRKMFFAMASDIRVIIIKLADRLHNMRTLMYMPEEKQRR
                     IAKETLDVYAPLAHRLGLQRI"
     gene            complement(571..1089)
                     /locus_tag="ACZPE2_07610"
     CDS             complement(571..1089)
                     /locus_tag="ACZPE2_07610"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR1319751.1"
                     /translation="MDLKEKFRHVKDFPKEGIDFIDITTVLGDGEAFKVAIDALAEKA
                     KATGCNVIVGSEARGFIVGAPVAYKLGIGFVPVRKKGKLPYETISGTYDLEYGTDTLE
                     MHVDAVKKGDKALIVDDLIATGGTVGCIADMIEKLGAEVSGTLFLVELTELDGRKKLE
                     EKGIKVDSVVEI"
BASE COUNT          247 a          312 c          241 g          349 t
ORIGIN      
        1 tatgcgctga agaccgagcc tgtgagcaag cggagcgtat acgtcgagcg tttcttttgc
       61 gatgcgtctt tgcttttcct cgggcatata catgagcgtg cgcatattat gcagcctgtc
      121 cgcaagcttt attattatga ccctgatgtc ggacgccatg gcgaaaaaca tcttcctgac
      181 gttttctatc tggcgctgtt cgtttgatac aaacggaatt ttgtcaagct ttgtaacgcc
      241 ctcgacaagg tcggcaataa cctggccgaa ctcgtttttc acggtattgt aattgtcctt
      301 ttcgacatcc tctatgacat cgtgcaacaa ccccgccgcg atgcattcgg agtccatttc
      361 aagctcggat aaaattatcg ccacgccaac cggatgaacg atatatgact cgccggaatt
      421 gcgcttctgt gacctgtgcg cctcctcggc atatttatag gctctttcga caagcgccgt
      481 gtccggcata tattttttca cattctcaag cagtttttca taggttgttt cgtgtgcctg
      541 catagtttgc tacgcccctt ttgaaagcaa ttatatctca actactgaat ctactttaat
      601 tcccttttcc tcaagctttt tgcggccgtc aagctcagta agctcgacca agaagagtgt
      661 cccggaaacc tcggctccga gcttttctat catatcggca atacaaccga ccgtgccgcc
      721 cgtcgctata aggtcgtcaa ctatcaatgc cttatcgcct tttttcacgg cgtcaacatg
      781 catctcgagc gtgtccgttc cgtattcaag gtcatatgtt ccgctgatgg tctcataagg
      841 aagcttacct ttttttctga ccgggacgaa cccgataccg agcttgtatg caaccggtgc
      901 gccgacgata aagccgcgcg cttcgcttcc gacgatgacg ttgcaacccg tcgccttcgc
      961 tttttcggcc aatgcgtcga ttgctacctt aaaagcctcg ccgtcgccca aaaccgttgt
     1021 aatgtcaata aagtcgattc cctccttcgg gaaatccttt acatgacgga atttttcttt
     1081 taaatccatt gtttcttcct cctacaatat gtcttttatt ataagctgcg gtctgccgtt
     1141 atagtcgtt
//
LOCUS       DBMDVO010000581         1147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57850_length_1147_cov_0.724665, whole genome shotgun sequence.
ACCESSION   DBMDVO010000581 DBMDVO010000000
VERSION     DBMDVO010000581.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57850_length_1147_cov_0.724665"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(211..633)
                     /locus_tag="ACZPE2_07615"
                     /pseudo
     CDS             complement(211..633)
                     /locus_tag="ACZPE2_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(663..>1147)
                     /locus_tag="ACZPE2_07620"
     CDS             complement(663..>1147)
                     /locus_tag="ACZPE2_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319752.1"
                     /translation="YIGILIIVCAVSFTVLCFLVVKNHRGRFKSKGTRIFLRVLSIII
                     CIMLCLAGITIDGILYTDTVKKATSDDKRHTIIIDSEPGSGEWNMTVYRRYSPFLMSA
                     RGNGKKVVDMADENGDIDVVWTDGGCLVSYKAYKTDATSVDDTETVTLRFFYNEKSGK
                     "
BASE COUNT          330 a          254 c          199 g          364 t
ORIGIN      
        1 cattacgtgg aattagttgt ccgtcctcat cataacataa ccaattatca tactcccgca
       61 aaaaatgtat ccacagttca cttttatggt tggttatcca gtttctgtat tcattcaact
      121 ttttcacata tgcttttaat acgatcgctt tttcttcatt cgtactcata cgctttttct
      181 ccttgtttct ttgaacttac tcccttatat tatttttttc ttttctatca taaggaatgt
      241 tcctgtgccg ataagaacca tacctattat tttaactgcg gtcagctttt ctccgagaaa
      301 aatcagggca agtatcattg taagtaccgt acttgactta tcgattggag ttaccttgtt
      361 tacgtcgccg agacgcaaag ccttaaaata gcaaagccaa gaagcccctg tggacagtcc
      421 cgataaaatc aaaaatataa acgttttgac gcttatatcc tttatttcgg atgctgatcc
      481 gacaataaaa accatcagcc acgaaaacaa cagcacaact attgtgcgca gagccgtggc
      541 aagatttgag ttgacgttcc gtaagccgat tttagcaaga attgccgtta ttccggcaaa
      601 tccggcagat aaaaatgcta aaacgagcca cataaaaaca cctcttataa gcagctgtat
      661 aattatttgc ctgatttttc attgtaaaaa aatcgcagcg ttacggtttc ggtgtcatca
      721 acgcttgttg catctgtttt ataagccttg taacttacaa gacagccgcc gtcggtccat
      781 acaacgtcta tatctccgtt ctcgtctgcc atatcaacga ctttttttcc gtttccgcga
      841 gcggacataa gaaaagggga gtatcgtctg taaacggtca tattccattc acccgagccc
      901 ggttcgctgt ctattataat agtatggcgc ttgtcatccg atgtggcttt ttttacggta
      961 tcggtgtata atattccgtc aattgtaata ccggcaaggc aaagcattat gcaaattatt
     1021 atcgaaagca ctcttaaaaa aatccgtgtt cctttgcttt taaaacggcc tcggtgattt
     1081 ttaacaacaa gaaagcataa gaccgtgaag ctgacggcac atactataat aagtataccg
     1141 atataag
//
LOCUS       DBMDVO010000582         1146 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57893_length_1146_cov_1.098565, whole genome shotgun sequence.
ACCESSION   DBMDVO010000582 DBMDVO010000000
VERSION     DBMDVO010000582.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57893_length_1146_cov_1.098565"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="ACZPE2_07625"
     CDS             complement(<1..716)
                     /locus_tag="ACZPE2_07625"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR1319753.1"
                     /translation="MLHIGCHLSSSGGFYKMGETALKIGADTFQFFTRNPRGSKAKEI
                     DPDDAKKLLELMEENRFPCILAHAPYTLNPCSANEDTRQFALDTMADDLRRMEFTPNQ
                     MYNFHPGSHTGLGVENGIELISCQLNRILTKGQTTTVLLETMAGKGTEVGKSFDELRE
                     ILDRIELNEKVGVCLDTCHVFDAGYDIVNSLDEVLTDFDRIIGLEKLRAIHINDSKNP
                     LGSHKDRHECIGKGYIGLDA"
BASE COUNT          297 a          262 c          242 g          345 t
ORIGIN      
        1 atggcgtcaa gcccaatata gcctttacct atgcattcgt gtctatcctt atgactgccg
       61 agcggatttt tactgtcatt tatatgtatg gctcgaagct tttcaaggcc tattattctg
      121 tcaaaatccg taagcacctc gtcaagagag tttacaatat cgtagccggc gtcaaaaacg
      181 tggcacgtgt caaggcacac gccgactttt tcattcaatt cgatacggtc gagtatttct
      241 cgaagctcat caaagctttt gccgacctct gtgccctttc ctgccatggt ttccaaaagc
      301 acggtggtag tctgcccctt tgtcaatatc ctgttaagct gacacgatat cagctcaata
      361 ccgttttcaa ctccgagacc tgtatgactg ccggggtgaa aattgtacat ctgattcgga
      421 gtgaattcca tcctcctaag gtcgtcggcc attgtatcaa gcgcaaattg cctcgtgtct
      481 tcgtttgcgg agcacgggtt caaggtgtaa ggagcgtgtg ccaaaataca gggaaaacga
      541 ttttcctcca taagctcaag aagctttttt gcgtcatccg ggtctatttc ctttgctttg
      601 cttccgcgcg gattgcgcgt gaaaaactga aacgtgtcag cacctatttt caaagcggtt
      661 tcacccattt tgtaaaagcc tcccgatgat gagaggtggc agcctatatg gagcatagct
      721 ttgcaccgtc cttaattctt tgttgaattt aattttatca taaattgccc tacgacagca
      781 agagcaacac gaagtgcggc ggcgaaagcc gcaatttagc tgttcattgt tctccgaaac
      841 ggtcaaaatc tttgatttgg tttttgtttc gtgaggttat tttaacataa aattctgcac
      901 tgtaagaata taaaaagtcg agcagccaag actttacctt taccgctcga ctttgctttt
      961 cggttcgctt cttgcccgct tatacaagca catacatata tatagcgaga aagtgaagaa
     1021 cagagcctat tattataaag aaatgaaaaa ccgtgtggaa ataacgcttt ttgctgccta
     1081 tgccgtagag aatgactccg agtgtatatg ctatactgcc tgaaatcagg agtataactc
     1141 ctgttc
//
LOCUS       DBMDVO010000583         1145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57979_length_1145_cov_0.893678, whole genome shotgun sequence.
ACCESSION   DBMDVO010000583 DBMDVO010000000
VERSION     DBMDVO010000583.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57979_length_1145_cov_0.893678"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="ACZPE2_07630"
     CDS             <1..637
                     /locus_tag="ACZPE2_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1319754.1"
                     /translation="LLRITKVAVPSILQQSFVSVGNVFIQGLINSFGAAVVAGFAAAM
                     KLNTFTITTLVTLSNGISSYTAQNIGAHNTERVKKGFGSGLKIALLVAIPFFIAFFIF
                     PKYMMMIFVTHNNTDVITAGVQFLKIVSPFYFLATVKIIVDGILRGSGAMLSFMVSTF
                     TDLILRVIFAFILTPHIGQLGIWWSWPIGWIVASLASLALYLSGVWKKAKV"
     gene            complement(705..>1145)
                     /gene="spo0A"
                     /locus_tag="ACZPE2_07635"
     CDS             complement(705..>1145)
                     /gene="spo0A"
                     /locus_tag="ACZPE2_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HIR1319755.1"
                     /translation="MNMMAERITQLTGWRDNSKFGKLHPADSSADLEVVVSDIMHQIG
                     VPAHIKGYQYLREAIILSINDSEMMNSVTKVLYPTVAKTFSTTSSRVERAIRHAIEVA
                     WDRGDVDVLSSYFGYTIQNSRGKPTNSEFIAMISDKLRLSMKIS"
BASE COUNT          331 a          234 c          222 g          358 t
ORIGIN      
        1 gttgctgaga attacaaagg ttgccgtacc cagcatattg cagcaaagct tcgtctcggt
       61 aggcaatgta ttcatacaag gccttattaa tagtttcggt gctgccgttg ttgcaggttt
      121 cgctgcagca atgaagctga atacatttac aataacaaca cttgtcacat taagcaacgg
      181 catttcaagc tacacagcgc aaaatatcgg agcacataat accgaaagag taaagaaagg
      241 gttcggatcg ggcctaaaaa tcgcattatt ggttgctatt ccgtttttta tagcattttt
      301 catttttccg aaatatatga tgatgatatt tgtcacacac aataataccg atgttataac
      361 agccggcgtc cagtttttaa aaatcgtatc accgttctat ttcttagcaa cagttaaaat
      421 aattgttgac ggcatattac gtggatccgg agctatgcta agctttatgg tttcaacctt
      481 caccgacctc atacttcgcg ttatattcgc atttattctc acaccgcata tcgggcaact
      541 cggaatttgg tggtcatggc cgataggttg gatagtcgca agcttagctt ctcttgcgct
      601 gtatctctca ggcgtgtgga aaaaagcaaa ggtttaatca aatatttata acaaaaaatg
      661 accgctcggc aaattaatcc gggcggtcat tttaattaac tgttttatga tattttcata
      721 ctgagtctta atttatcgct tatcatggca ataaactcag agttagtggg ctttccgcga
      781 gagttttgta ttgtatagcc gaagtaagaa ctaagaacgt caacgtcgcc tctatcccat
      841 gcaacttcta ttgcgtggcg tatggcacgt tcaactcttg aagaagttgt tgaaaaagtt
      901 ttagcaacag ttggatacaa aacttttgtt acagagttca tcatttcgct gtcgtttatt
      961 gataaaataa ttgcctctcg caaatactga taacctttta tatgagccgg aacaccaatt
     1021 tgatgcataa tatcgctgac aacgacttcc aaatcagcag aactgtcagc agggtgaagt
     1081 ttcccgaact tcgaattgtc tctccagcct gtcaactgag ttattctttc agccatcata
     1141 ttcat
//
LOCUS       DBMDVO010000584         1145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_57980_length_1145_cov_0.887931, whole genome shotgun sequence.
ACCESSION   DBMDVO010000584 DBMDVO010000000
VERSION     DBMDVO010000584.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57980_length_1145_cov_0.887931"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="ACZPE2_07640"
     CDS             complement(<1..>1145)
                     /locus_tag="ACZPE2_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR1319756.1"
                     /translation="ETDTQPTDAGYYTIKAYVEESDFYNYVEETKQFIIKDKNEIKVF
                     DFPDEWYFGEDEKIPNGSAKFGLVNFEYSVDGGESWTTEQPKSAGSYLIKAYVDGSDN
                     YSDAEETKSFTIKQAVNEITEFWEINDWTYGDSVIIINTNKAKFGEPQLVFSADGENW
                     TTEIPKNAGQYIAKAYVEETNDYPYAEKTQNFEVLKATPTVTELPVAEKVKRNRQLST
                     SAISGGEVTGVDTWFTDGVLSGIWSWKNGSEKMKSTGDFTRMAVFTPDSLNYNPVEAE
                     INVHVYMPTTGGSSYTLPKVENDTTKPEENKKIILTVGSKNAIVFGESVVNDVAPVIV
                     NERTMLPARFVAEALGAKVLWDSNTRTVSIIGEKAIAITIGSDTAYV"
BASE COUNT          254 a          286 c          202 g          403 t
ORIGIN      
        1 tttacatacg ccgtatcact gccgattgta atggctatcg ccttttcgcc gataattgaa
       61 actgttcttg tattgctgtc ccaaagcacc tttgcaccga gtgcctctgc aacaaatctt
      121 gccggcaaca ttgttctttc gtttactata accggcgcta catcgtttac aacgctttca
      181 ccgaatacaa tagcattttt acttccgacc gtgagtatta ttttcttgtt ttcttcaggc
      241 ttagttgtat cattttcaac cttcggcaga gtatatgatg agccgcctgt tgtcggcata
      301 taaacatgta cgtttatttc tgcctcaacc ggattatagt ttaagctgtc cggcgtgaac
      361 actgccattc ttgtgaaatc gcctgttgat ttcatctttt cactgccatt cttccatgac
      421 catattccgg aaagaacgcc atccgtaaac catgtatcaa cacctgttac ctcaccgccg
      481 cttatagccg aagttgaaag ctgtctgttt ctctttacct tttcggcaac aggaagttcc
      541 gttactgtcg gtgttgcctt aagcacctcg aaattctgtg tcttttccgc atagggatag
      601 tcatttgttt cctctacata tgcctttgcg atatactgac ctgcattttt gggtatttca
      661 gttgtccagt tttcgccgtc agcggaaaat acaagctgag gctctccaaa tttcgcctta
      721 ttggtattta ttataataac cgaatcaccg taagtccagt catttatctc ccagaactcc
      781 gttatctcgt ttactgcctg cttaatagta aagctctttg tctcctctgc atcgctataa
      841 ttgtcgcttc cgtcaacata cgcttttata aggtagcttc cggctgactt cggctgttcc
      901 gttgtccagc tttcgccgcc gtcaacggaa tattcgaaat taacaagacc gaattttgcc
      961 gagccgttag gtatcttttc atcttcgccg aagtaccatt catcgggaaa atcaaagact
     1021 tttatttcgt ttttgtcctt tattataaac tgctttgttt cttccacata gttataaaaa
     1081 tcgctttcct ctacatacgc ctttattgtg taatatcctg cgtctgtcgg ctgtgtatcc
     1141 gtttc
//
LOCUS       DBMDVO010000585         1144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58027_length_1144_cov_1.318313, whole genome shotgun sequence.
ACCESSION   DBMDVO010000585 DBMDVO010000000
VERSION     DBMDVO010000585.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58027_length_1144_cov_1.318313"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="cdaA"
                     /locus_tag="ACZPE2_07645"
     CDS             complement(<1..874)
                     /gene="cdaA"
                     /locus_tag="ACZPE2_07645"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1319757.1"
                     /translation="MLQLLNFKDFWSEIGRAVMSFNSISDVLDVILVTFVIYSAIKLI
                     RETKAVQLAKGFILLLIVYALVNLFGMQASNYILSLVFNNLLLVLVIIFTPEIRHALE
                     SVGRSSVSKFNFFGFRSGAEEKRREEMQKMVNAVCRASSDMSDKHIGALMVFEQETIL
                     GDIISSGTVIDAKVSPEMIGNIFYPGAPLHDGAAVFRDGRICAAGCILPLTPNHDISS
                     ELGTRHRASIGMSEQSDAVIVVVSEETGAISVAYKGRLRRGISDGDLREILMEHFVKP
                     EEDEGNSKIKKWLKG"
BASE COUNT          330 a          286 c          219 g          309 t
ORIGIN      
        1 ttcctttcaa ccatttctta attttcgaat ttccttcgtc ctcttccggc ttaacaaaat
       61 gctccataag tatctctcga aggtcaccgt ccgaaatgcc tctgcggagc ctgcccttat
      121 atgccacgga aatggctccg gtctcctcgg atacaacgac tataacagca tcgctttgct
      181 cgctcatacc gatggatgct ctgtgacgtg ttccgagctc gctgctgata tcgtgattcg
      241 gtgtcagcgg aagtatacag cctgcagcgc aaattctgcc gtcgcggaaa accgcggcac
      301 cgtcgtggag cggtgcgccc ggataaaaaa tgtttcctat catttcggga gaaaccttgg
      361 catcgataac cgtacccgac gaaataatgt cgcccaaaat agtctcctgc tcaaaaacca
      421 taagcgcgcc tatatgctta tcgctcatat cggagcttgc gcggcatacg gcattgacca
      481 ttttctgcat ctcttcacgt cgtttttctt cagcacccga acggaatccg aaaaaattga
      541 atttcgacac cgagcttctg ccgacgctct cgagcgcatg acgaatttcg ggagtaaaga
      601 tgataacaag caccaacaaa agattattga aaacaagcga caaaatatag ttactcgcct
      661 gcataccgaa caaattgaca agagcatata ctattaggag caaaataaat cccttggcaa
      721 gctgaaccgc ctttgtttca cgtatcagct ttattgcgct gtatataacg aaggtaacaa
      781 gaatgacatc aagcacatcc gaaatgctgt taaagctcat aaccgctctg cctatttcgc
      841 tccaaaaatc tttgaaattc aaaagctgca acatacttta cacctgtttc aataaaaaaa
      901 tacaatatat cgtaattaaa agctatcatc tgccgccctt gcggcaaata aaataatcat
      961 aaataatcac gatatattgt atcacaatca actgtttttt gcaattttta ctacggaagt
     1021 taacaataaa ttcacatcct gcttttttgt gaatattgac ggtgctattc tgactgtacc
     1081 ttcgcgaaga gttccgtacg ttttgtgagc cgacggtgcg caatggagac ctgctctgac
     1141 ggca
//
LOCUS       DBMDVO010000586         1144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58068_length_1144_cov_0.686481, whole genome shotgun sequence.
ACCESSION   DBMDVO010000586 DBMDVO010000000
VERSION     DBMDVO010000586.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58068_length_1144_cov_0.686481"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="ACZPE2_07650"
     CDS             complement(<1..>1144)
                     /locus_tag="ACZPE2_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR1319758.1"
                     /translation="QKPESGKIYTALYCDGVLKSIKEIDSDDIQFDARGVYETTVTMD
                     VPKVGGNYTAKVFVWDDSMRPIGDVYETEYNVESPFALPNVFSDNMMLQADEDITVWG
                     TGVPNDTVTVTLKEKDAETAYEANCTIAEDGTWEVTLDAKECGGDYTMTVKCGEETRR
                     YTNIIFGDVYLLAGQSNMEAWLSWIDSQNYAGEKERAENSNIRTIDLLSKGGNGSSNP
                     SDNLPEIEGNAWAPMTFSNANTTSAIGYYFAQELNKETGRPIGYIHAAVGGTRIDRWL
                     ENDYVEGISSPWSLYNNRVYPLRKFKLSGVLYYQGESDGPEDADSLDSSKGMSADQYS
                     TIMAALIDNYRELFNNENLPFYYAQLARYSNQNFENIRAAQVWALDK"
BASE COUNT          297 a          290 c          182 g          375 t
ORIGIN      
        1 ccttatccaa cgcccatacc tgagccgcac ggatattttc aaaattctga tttgaatatc
       61 ttgcaagttg cgcataatag aacggaagat tttcgttatt aaacagttct ctgtaattgt
      121 caataagtgc cgccattatc gtactgtatt gatctgcact cattcccttg ctactatcca
      181 agctgtctgc atcctcaggt ccatcggact cgccctgata atacagaact cctgacagtt
      241 taaactttct taaaggataa acacggttat tgtataatga ccacggcgaa gaaattcctt
      301 caacataatc attttcaagc catctgtcta tccttgtacc tcctacagca gcatgaatat
      361 aacctatcgg tctgcctgtt tctttattaa gttcctgtgc aaaataatat cctattgcag
      421 aagttgtatt tgcatttgag aatgtcatcg gtgcccatgc atttccttca atctccggaa
      481 gattatcgct aggattcgaa gaaccgtttc cgcccttgct taacaaatca atagttctga
      541 tattagaatt ttcggctctt tccttttctc ctgcataatt ctggctgtca atccaactca
      601 accatgcttc catatttgac tgaccggcaa gcagataaac atcgccaaag ataatatttg
      661 tatatctcct tgtttcttcg ccgcatttaa ccgtcattgt atagtcaccg ccgcattcct
      721 ttgcatcaag tgtaacctcc caagtgccgt cctcagcaat tgtgcagttt gcttcatatg
      781 ctgtttctgc gtccttttcc ttaagagtaa cggttacagt atcgttcgga acacctgtac
      841 cccagacagt tatatcctca tctgcctgaa gcatcatatt atcggaaaat acattcggca
      901 aagcaaatgg gctttcaaca ttatattctg tttcatatac gtcacctatc ggtctcatgc
      961 tgtcgtccca tacaaatacc ttcgcagtat aatttccgcc taccttcggt acgtccattg
     1021 taaccgtagt ttcatacacg cctcttgcgt caaactgaat atcatcgctg tctatttctt
     1081 ttatcgactt aagaacacca tcacagtaaa gcgccgtata aatttttccg ctttcaggct
     1141 tctg
//
LOCUS       DBMDVO010000587         1142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58199_length_1142_cov_1.103746, whole genome shotgun sequence.
ACCESSION   DBMDVO010000587 DBMDVO010000000
VERSION     DBMDVO010000587.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58199_length_1142_cov_1.103746"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="mnmL"
                     /locus_tag="ACZPE2_07655"
                     /gene_synonym="ytqA"
     CDS             complement(<1..591)
                     /gene="mnmL"
                     /locus_tag="ACZPE2_07655"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393234.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR1319759.1"
                     /translation="MRRYRSLNDYLKDTFGEKVYKLALDGGMTCPNRDGTKGTGGCIF
                     CSEGGSGDFAERYTGNIETQITDAISRISGKTKAEKFIAYFQSYTNTYAPRPYLEKLF
                     CTAVEDERIAALSIGTRPDCLPPDIIGLLDELNKIKPVFVELGLQTSNEATGKLIRRG
                     YPLCVYDEAVYKLKSIGVNVVTHMIIGLPHETTEDMK"
     gene            complement(588..>1142)
                     /locus_tag="ACZPE2_07660"
     CDS             complement(588..>1142)
                     /locus_tag="ACZPE2_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319760.1"
                     /translation="RLTEADFLHDDFHSLGDAVLAEFKDGKIIFSNDSRLPSQEELSK
                     PLPGITDGEFELSGRVVLLKSYPGLDYSELRIKHDNVCAVVNVTYHTGSAPCAGGSFG
                     LPEFCDECHKNSIDVYLAGLRRTDDIYETSKQIYEHGAEPIYSVSVPAAVSKLRAAYN
                     SSLKNIDTLISNDIYYESLPQEEK"
BASE COUNT          275 a          287 c          260 g          320 t
ORIGIN      
        1 tttcatatcc tcggtcgttt catgcggcaa gcctattatc atatgcgtca caacattcac
       61 acctatgctt ttaagcttat atacggcttc gtcgtaaaca cacagcggat agccccttcg
      121 tataagtttt cccgttgcct cattcgaggt ctgaagccca agctcgacaa agacaggctt
      181 tattttgttc agttcgtcca aaagaccgat tatatccggc ggcaggcaat cgggtcttgt
      241 gcctattgac agagccgcta tgcgttcgtc ctcgacagcc gtacaaaaca gcttttcgag
      301 atacggacgc ggtgcatagg tgtttgtgta gctttgaaaa taggctataa atttctccgc
      361 tttagtcttg ccgcttatac gtgatatagc atcggtgatt tgtgtctcta tattgccggt
      421 atatctttcc gcgaagtctc cgctgccgcc ctcgctgcaa aatatacagc cgcctgttcc
      481 ttttgtcccg tcgcggttcg gacaggtcat accgccgtca agggctaatt tatacacctt
      541 ttcgccgaag gtatccttaa gataatcgtt taacgaacgg tagcgtctca tttttcttcc
      601 tgcggcaatg attcgtaata aatatcattt gaaatcagcg tatcaatatt tttgagagac
      661 gaattatatg ccgcgcgaag ctttgaaacg gcagccggca cgctgacaga atagataggc
      721 tctgcaccgt gctcgtaaat ttgctttgac gtctcgtata tgtcatctgt ccttctgagc
      781 ccggcaagat atacgtctat actgtttttg tggcactcgt cgcaaaattc gggaagcccg
      841 aaggagccgc ccgcgcaggg tgccgagccc gtatgatatg taacattaac aaccgcacag
      901 acattatcat gctttatgcg aagctcggaa tagtcaagcc ccggatatga ttttaagagc
      961 accactcttc cggacagctc gaactctccg tccgttatac cgggaagagg ctttgaaagc
     1021 tcctcctgag acggcagacg cgaatcattt gagaatataa ttttaccgtc cttgaattct
     1081 gccaaaacgg catcgccgag cgaatggaaa tcgtcgtgaa ggaagtcggc ttcggtgagc
     1141 ct
//
LOCUS       DBMDVO010000588         1141 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58299_length_1141_cov_0.892308, whole genome shotgun sequence.
ACCESSION   DBMDVO010000588 DBMDVO010000000
VERSION     DBMDVO010000588.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58299_length_1141_cov_0.892308"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="ACZPE2_07665"
     CDS             <1..300
                     /locus_tag="ACZPE2_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1319761.1"
                     /translation="AIGSGLAAVAGVLLCSAYPTLSSQTGAMPGIKAFVAAVLGGIGS
                     IPGAVIGGVLIGVIEILSRSYISSQMADAIVFAVLIIVLLVKPTGILGKKYIEKV"
     gene            314..>1141
                     /locus_tag="ACZPE2_07670"
     CDS             314..>1141
                     /locus_tag="ACZPE2_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1319762.1"
                     /translation="MASTKSKLIKKSTRSDLITYAMVLVVFAIVEILKSTGNISSLLR
                     GLLVPLCMYSILAVSLNLVVGYLGELSLGHAGFMCVGAYASSIFSVVTQNTLTNAWIR
                     FPLAILVGGLCAAIFGVLIGIPVLRLKGDYLAIVTLAFGEIIKNILGTIYVGYDSHGI
                     HASFTSQSSLNLEADGTVLINGPLGITGTPNDSTFVIGIVLLLITLFVILNFIHSRAG
                     RAVMSLRDNRIAAESVGISVTKYKLIAFSLSAFFAGIAGVLYSHNLSVLAATSKNFGY
                     "
BASE COUNT          260 a          268 c          275 g          338 t
ORIGIN      
        1 gccataggct cggggctggc ggccgttgca ggcgtgctcc tttgctcggc ttatcccaca
       61 ctttcgtcgc agacaggcgc aatgcccggc attaaggcat tcgtagctgc cgttctcggc
      121 ggtatcggtt ctattccggg cgccgtaatc ggcggtgtgc ttataggcgt tatcgaaata
      181 ctcagccgct cttatatttc ttcgcagatg gctgacgcta tcgtgtttgc cgtacttata
      241 atagtgcttc ttgttaagcc gacgggcatt ctcggcaaga agtatattga aaaggtttaa
      301 ggagggtaca aagatggcat caacaaagtc aaaactgatc aaaaaaagta cccgaagcga
      361 cctcataacc tacgctatgg ttttagttgt atttgccata gtcgaaatac tcaagtccac
      421 gggaaatata tcgagccttt tgaggggcct tttggttcct ctgtgtatgt actccatact
      481 tgccgtttct cttaaccttg tagtcggtta tctcggtgag ctttcgctcg gccatgcggg
      541 atttatgtgc gtcggtgcgt atgcaagctc gattttctcg gtagttacgc aaaacactct
      601 tacaaatgca tggattcgtt ttccgctcgc catacttgtc ggcggacttt gcgcggcaat
      661 attcggcgtg cttataggca tacccgtttt aagattaaag ggcgactatc ttgcaatagt
      721 aacgcttgcg ttcggcgaga taattaagaa catactcgga acaatatacg tcggatatga
      781 cagtcacggt attcacgcat cctttacgag tcagtcgtcg ttgaatcttg aggctgacgg
      841 tactgttctt ataaacgggc cgctcggtat aaccggaaca cccaacgatt caacatttgt
      901 aataggtata gttcttcttc ttatcacgct gtttgtaatt ctgaacttta ttcattcgcg
      961 cgccggaaga gcggtaatgt ctctgcgtga caatcgtata gccgccgaat cggtgggtat
     1021 aagcgtgact aaatataagc ttatcgcctt ttcgctttcc gcattttttg caggcatagc
     1081 cggcgttttg tattcgcata acctcagcgt gcttgccgca acatcaaaga atttcggcta
     1141 c
//
LOCUS       DBMDVO010000589         1138 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58498_length_1138_cov_1.005786, whole genome shotgun sequence.
ACCESSION   DBMDVO010000589 DBMDVO010000000
VERSION     DBMDVO010000589.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58498_length_1138_cov_1.005786"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(69..722)
                     /locus_tag="ACZPE2_07675"
     CDS             complement(69..722)
                     /locus_tag="ACZPE2_07675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR1319763.1"
                     /translation="MFVFVLINYKELVNIDVREIVSAAPSLLPAVLSVLAIYCVKGLV
                     LVIPASLVYISVGMAFSPLTAVLVNIAGILVEFTASYLFGLFLGGDYINKMMQKQKYG
                     KKILEMQENKKDASVFLMRFITVFPLDFVSLFLGSSKYNFGKYILFSFLGLAPRVILF
                     TILGDKIYDYIPMKLIMTICLFAIPVAVIAIIIKAAVDKKRKKNESNENTADKAEMK"
     gene            complement(940..>1138)
                     /locus_tag="ACZPE2_07680"
     CDS             complement(940..>1138)
                     /locus_tag="ACZPE2_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR1319764.1"
                     /translation="IKISEATDKLPPNFVRLHKSFIVNFDYIAAIYPYAARLRDGTEI
                     SIPQKKYKTVKEMFISYVDAG"
BASE COUNT          355 a          255 c          202 g          326 t
ORIGIN      
        1 gtttcttatc aacgcttttt aaatatatcg gatattatac ttccgcaagc gcaatttgtc
       61 aaatcatatc atttcatctc ggctttatcg gccgtatttt cgttactttc gttctttttg
      121 cgctttttgt caaccgcggc tttaattatt atcgctataa ccgcaacagg aatggcaaaa
      181 agacatatgg tcattatgag cttcattggg atataatcat atattttgtc accgagaatc
      241 gtaaacaata ttactctcgg agcaagcccc aagaacgaaa acaaaatgta ctttccgaaa
      301 ttatacttac tgctgccgag aaacaggctt acaaaatcaa gcggaaacac ggttataaag
      361 cgcataagga aaacggacgc gtccttttta ttttcctgca tttcaagtat ctttttaccg
      421 tacttttgct tttgcatcat cttatttata taatcgccgc cgaggaaaag cccgaagagg
      481 tatgacgccg taaattcaac aagtattccg gcgatattca caagcaccgc cgtcaaaggt
      541 gaaaatgcca taccgaccga aatatagacg agtgacgccg gaataacaag cacaagcccc
      601 tttacacaat atatcgcaag aaccgacaat acggccggca aaagcgacgg agccgccgaa
      661 acaatttcgc gtacatctat atttacaagc tctttatagt ttataagcac aaaaacaaac
      721 acggccaacg ccaccgctat ttttgatatc agctttatca gttctttttt attttctttc
      781 agatgccgct tttcgccttc ttttttatag ctttaaacca agcggaagcg atgaccctcg
      841 cgcccacgcg gaaacaacag tagtatacca cagaaaaacg atttggcaaa caaaaagaaa
      901 aaatatgtac agaatttttt acagcggcgg ctgaacagct tacccggcat cgacatatga
      961 aataaacatt tccttaacgg tcttatactt tttttgagga atcgaaattt cggttccgtc
     1021 acgaagtctt gcggcatacg gatatatcgc ggcaatataa tcaaaattga caatgaaact
     1081 cttgtgcagt cgaacgaaat tcggcggcaa tttatcggtc gcttcgctta ttttaata
//
LOCUS       DBMDVO010000590         1138 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58506_length_1138_cov_0.843780, whole genome shotgun sequence.
ACCESSION   DBMDVO010000590 DBMDVO010000000
VERSION     DBMDVO010000590.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58506_length_1138_cov_0.843780"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..687
                     /locus_tag="ACZPE2_07685"
     CDS             145..687
                     /locus_tag="ACZPE2_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1319765.1"
                     /translation="MDMTEKTLKQDYKFRGRIVNMRVDEALMPNGTTASREVVEHNGG
                     VCVAPLTDDGELIFVRQFRYPYSEVLLELPAGKLEKGEDPFLAGKRELKEETGATARS
                     YISLGNLYPSPGYCGEIIHMYLAKGLTFGEQHLDKDEFLEVVKIPLQKAFEMVMNNQI
                     TDSKTQVGIVKAYYLENGKY"
     gene            752..>1138
                     /locus_tag="ACZPE2_07690"
     CDS             752..>1138
                     /locus_tag="ACZPE2_07690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1319766.1"
                     /translation="MKLILASGSPRRSEILKNAGYEFVVKPTSADESLPGGILPEDAV
                     KLLSERKADAAKIECSDVVLSADTVVALGDRILGKPSDKAEAFSMLRSLSGKTHSVYT
                     GVTIKSAEKKRVFAVKTDVLFYPLTDD"
BASE COUNT          328 a          251 c          293 g          266 t
ORIGIN      
        1 tcatttgaac cgtgattttg tcatcagcgg atatttaccg gcgaatcatt tttatcaact
       61 gctttctttt tattctcgcg ctctgcccga aatcatatcg ggtataacac tatcaatata
      121 tattccgaac ggagtggaaa tattatggat atgaccgaaa aaaccttaaa acaggattac
      181 aaatttcgcg gacgcattgt aaatatgcgc gttgacgagg cgttgatgcc caacggaaca
      241 acggcttcac gtgaggtagt ggagcacaac ggcggggttt gcgttgcgcc gcttacagat
      301 gacggggagc tgatcttcgt aagacagttt cgctacccgt attccgaggt gcttttggag
      361 ctgccggcag gtaagcttga aaaaggcgag gacccgttcc tcgcaggcaa gcgcgaatta
      421 aaagaagaga ccggcgctac tgcacgcagc tatatttcgc tcgggaattt gtatccgtca
      481 cccggctatt gcggagaaat aatacatatg taccttgcaa aaggactcac cttcggcgag
      541 cagcatcttg ataaggacga gttccttgag gttgtgaaaa tccctctgca aaaggcgttt
      601 gaaatggtta tgaataacca aataaccgac agcaagacgc aggtcggaat agtaaaagcc
      661 tattatctcg aaaacggaaa gtattgacga aacgaaaggc attgaccaaa gcgctttctg
      721 taaacaacaa taaaattccg gaggataaaa aatgaagctg atacttgcgt cgggctctcc
      781 gagacgctcc gaaatactta aaaacgcagg atatgaattt gtcgtaaagc cgacgtctgc
      841 ggacgagtct ctgcccggcg gcatactccc tgaggacgcg gtaaagctct tatcggaaag
      901 aaaggccgac gccgcgaaaa tcgagtgtag cgatgtggta ctttccgccg ataccgtggt
      961 ggctctcgga gacaggattc tcggaaagcc gagcgataag gccgaggcat tttcgatgct
     1021 gcgaagcttg tcgggaaaaa cgcacagcgt atatacgggc gtaacgataa agagcgccga
     1081 gaaaaaacgt gtgttcgcgg taaaaaccga cgttttgttt tatcctctta ccgatgat
//
LOCUS       DBMDVO010000591         1137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58550_length_1137_cov_1.100386, whole genome shotgun sequence.
ACCESSION   DBMDVO010000591 DBMDVO010000000
VERSION     DBMDVO010000591.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58550_length_1137_cov_1.100386"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="ACZPE2_07695"
     CDS             <1..932
                     /locus_tag="ACZPE2_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1319767.1"
                     /translation="TVYRTLTGGFYLEAGIYLEVNLVAESKVFKLRLEAPLIPEIKWP
                     LFSMGDKDLVICLNDITTPILFSSNGTYWGEINVNDLPAVSAQTLDITTGVINYNASV
                     PWSKLSMNVSGEGFRMLYDRRGRFIRYEQPYSMSGFYKNSTAEGVGQIHYTGTSLQFT
                     KTADPEMSLTKSVKVIWYDESRVKEEDVGKIFTVNFYREVDGVKTLVETRKVPAGSLA
                     GSYNTNNAPDSWMYIDCEWKDQDPYFIAVDHDNYEIVYTATRAQTLMEMEYYDTVNKE
                     WVVELWAVNAGDVLKVPDNSSDEYMKLTSWHAT"
     gene            957..>1137
                     /locus_tag="ACZPE2_07700"
     CDS             957..>1137
                     /locus_tag="ACZPE2_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319768.1"
                     /translation="MYKYAGDKLRAIDSGCRKDYYTSGQPTDKPLYKVTGNTQRAASE
                     NFYFNHHNDYQNVALH"
BASE COUNT          350 a          251 c          278 g          258 t
ORIGIN      
        1 gcacggtata ccggacgctg acgggcggat tctatcttga agcgggaatt tatctcgaag
       61 taaatcttgt cgcagaatca aaggtattca aattgcggct tgaagcaccg ctgataccag
      121 aaatcaagtg gccgctgttc tcaatgggcg acaaggatct tgttatatgc cttaacgata
      181 tcacaactcc gatattattc agctcaaacg gcacttactg gggagagatc aacgtgaatg
      241 atcttccggc agtgtccgca cagacgcttg atattacaac aggcgtgata aattacaatg
      301 cgtctgtacc gtggagcaag ctgtcgatga atgtttcggg cgaaggcttc agaatgctct
      361 atgacaggag aggaagattt atccgttacg agcagccata cagtatgtca ggcttctaca
      421 agaacagtac agccgaaggt gtcggtcaga tacactatac aggcacgtca cttcagttca
      481 caaagaccgc cgaccccgaa atgagcctta caaagtcggt aaaggttatc tggtacgatg
      541 agtcgagagt taaggaagag gacgtaggca agatattcac tgtcaatttc tacagagagg
      601 ttgacggagt taaaactctt gttgaaacaa gaaaagtacc tgcgggcagt cttgcaggct
      661 cttacaatac aaataacgct ccggacagct ggatgtacat tgactgcgag tggaaggacc
      721 aagacccgta cttcatagcc gttgaccacg ataattacga aatagtatac accgcaacaa
      781 gagcgcagac tcttatggag atggaatact atgataccgt aaacaaagag tgggtagtag
      841 aactgtgggc agtgaatgcc ggcgatgttc ttaaagtacc cgataactcc agtgacgagt
      901 atatgaagct gacctcatgg cacgcaacct aaggaatacc gttcggtttt gaaaagatgt
      961 ataagtatgc cggtgataag ctcagagcga tagactccgg atgccgcaag gattactata
     1021 caagcggtca gcctacagac aaaccgcttt acaaggttac aggcaacacc cagagagcgg
     1081 cttccgaaaa cttctatttc aatcatcaca acgactatca gaacgttgca ctccact
//
LOCUS       DBMDVO010000592         1133 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58814_length_1133_cov_1.052326, whole genome shotgun sequence.
ACCESSION   DBMDVO010000592 DBMDVO010000000
VERSION     DBMDVO010000592.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58814_length_1133_cov_1.052326"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="ACZPE2_07705"
     CDS             complement(<1..242)
                     /locus_tag="ACZPE2_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319769.1"
                     /translation="MKELTKRKKIIGTIETVLAAAVIIIAVVTAPKLSAQKEEETSAA
                     ASETAAATQAATETEHEKTEPTTSSVGIGVQPIGEYS"
     gene            complement(269..958)
                     /locus_tag="ACZPE2_07710"
     CDS             complement(269..958)
                     /locus_tag="ACZPE2_07710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1319770.1"
                     /translation="MIYITGDTHGEVERLVPSKLRALKEGDTLIVCGDFGFIWDGSKK
                     EQKILKQLGKRKYNICFIDGTHENFSLLNGYGVSEWNGGKVHKIYDNLYHLMRGQIYN
                     IDGKSVFTMGGGESPDIDIRSENDGWSRDEIPSQEELLEGAKNLEKHGFSVDIVVTHE
                     PPTRIKGFLQLKDYDMLRVTALNAYFEELSDSCKFSKWFFGSMHVDKYISGTHIAVFK
                     NIVNAETGEKV"
BASE COUNT          263 a          280 c          236 g          354 t
ORIGIN      
        1 gaatactcgc ctatcggctg aacgccgatt cctacggatg aggttgtagg ctcggttttt
       61 tcatgctcgg tttcggtggc cgcctgtgta gctgccgcgg tctcgctcgc cgcagcggag
      121 gtttcctcct ccttttgggc agagagcttc ggcgccgtaa cgaccgcgat tattataaca
      181 gccgccgcca gcaccgtttc tattgtaccg attatttttt tacgtttggt aagctctttc
      241 attcggtctt acttcttcct ccgaaaaatt aaaccttttc tccggtctcg gcatttacta
      301 tgtttttaaa cacggctata tgcgtacccg atatatattt atccacgtgc atactgccga
      361 agaaccattt tgaaaatttg cacgaatcgg aaagctcctc aaaatacgcg ttgagagctg
      421 taacacgcag catatcgtaa tccttgagct gtaaaaagcc cttgattcgc gtaggcggct
      481 cgtgggtaac aactatatca acggaaaagc cgtgcttttc aagatttttt gcaccctcca
      541 aaagctcctc ctgcgaaggt atttcgtcac gcgaccagcc gtcgttctcg gagcgtatgt
      601 caatatcggg gctttcgccg ccgcccattg taaacacgct tttgccgtca atattatata
      661 tttgaccgcg cataaggtgg tagagattat cgtaaatctt atgcaccttg ccgccgttcc
      721 actcgctgac tccgtagccg ttcagaagtg aaaagttctc gtgggtaccg tcaataaagc
      781 agatattgta ttttctcttg ccgagctgct ttaatatttt ttgttctttt tttgaaccgt
      841 cccatataaa accgaagtct ccgcaaacta taagcgtatc cccctcttta agggcacgga
      901 gctttgacgg gacaagtctt tcgacctcgc cgtgtgtatc accggtaata tatatcaaga
      961 ttttcacatc cttaatacaa ttataattga atttcagagc gtattttgct atactactta
     1021 tgatacactt tttcctaatt cttttcaagg gggattttgt aattgaaacg tgtcgcatca
     1081 ataatggtaa ccgcagttat acttagctgc tcactcgttt taccggcgag tgc
//
LOCUS       DBMDVO010000593         1133 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58836_length_1133_cov_0.786822, whole genome shotgun sequence.
ACCESSION   DBMDVO010000593 DBMDVO010000000
VERSION     DBMDVO010000593.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58836_length_1133_cov_0.786822"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="ACZPE2_07715"
     CDS             complement(<1..177)
                     /locus_tag="ACZPE2_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1319771.1"
                     /translation="MKNDNLLEIKDLHVSAGEKEILHGVNLTVGKDETHVLMGPNGTG
                     KSTLGYAVTGNPEYT"
     gene            complement(174..644)
                     /locus_tag="ACZPE2_07720"
     CDS             complement(174..644)
                     /locus_tag="ACZPE2_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1319772.1"
                     /translation="MMISSKGRYALRVMLDLAEQGSNKYISLKDIAERQDISRKYLES
                     IMTELSKNELVESAIGKTGGYKLKRAPENYKVGEILYAAEGELTPVSCLAKDGKKCDR
                     GDSCYTYPFWLGLEEHIKEYVNSYTLKDVLELKNKQKNSCCKCDSHNENEDDVI"
     gene            complement(918..>1133)
                     /locus_tag="ACZPE2_07725"
     CDS             complement(918..>1133)
                     /locus_tag="ACZPE2_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319773.1"
                     /translation="AKVRSTLATSKFEEFDKELVSDTGDYAKKVNKSVIKWTSKNQEK
                     LLKTYAVNSKYNSKTTSTTSSNASTLY"
BASE COUNT          298 a          263 c          208 g          364 t
ORIGIN      
        1 ggtatattcc ggattacccg ttacggcgta gccgagcgtg gacttacctg ttccgttcgg
       61 acccataaga acgtgtgttt catcttttcc gaccgtaaga tttacaccgt gaagtatctc
      121 tttttcacct gcactgacgt gaaggtcttt aatttcaagc agattgtcgt ttttcatatt
      181 acatcatcct cgttttcgtt gtgggaatca catttacagc atgagttctt ttgtttattt
      241 ttaagctcga gaacatcttt aagcgtatag ctgttaacat attcttttat atgctcctca
      301 agtccgagcc aaaacggata agtataacag gaatctcccc tgtcacactt ttttccgtct
      361 tttgcaagac aagatacggg agtaagctcg ccctcggcgg catataggat ttcaccgacc
      421 ttgtaattct ccggtgcacg ctttaattta tatccgccgg tttttccgat ggcactttcg
      481 acaagctcgt ttttagacag ctcggtcata atgctctcaa gatattttcg ggaaatgtcc
      541 tgtctctccg cgatatcttt aaggcttata tatttattgc tgccctgctc tgccaaatcc
      601 agcataaccc gcagagcata tctgcccttt gaagatatca tcataaaaag cactcctttc
      661 cacgccgttt cacaacagcg aaacagccga ttacagcaca aaagacagta aattgtttta
      721 atctcctatc tttccactgg caattatact tcccaactcc cactttgtca ataggaatta
      781 acgctgttta caaaaatgaa catattatat aaaaagtgta tttacacgca aaaaggcagc
      841 ccgcaaaagc ggactgccga aaagtttttt taaattacgc tcaacgaaca cgataaatcg
      901 tgcaaaacgt ctcactctta atagagggtg cttgcgtttg agcttgtagt agatgtagtc
      961 ttgctgttat actttgagtt aacggcatag gttttgagaa gcttctcctg gttcttagag
     1021 gtccacttaa tcacagactt gttaaccttt tttgcatagt ctcccgtatc ggaaacaagc
     1081 tctttatcaa attcctcgaa cttagaggtt gcaagagtgc ttctgacctt tgc
//
LOCUS       DBMDVO010000594         1131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_58953_length_1131_cov_1.019417, whole genome shotgun sequence.
ACCESSION   DBMDVO010000594 DBMDVO010000000
VERSION     DBMDVO010000594.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58953_length_1131_cov_1.019417"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    468..477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <478..>1131
                     /locus_tag="ACZPE2_07730"
     CDS             <478..>1131
                     /locus_tag="ACZPE2_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6TM ABC transporter family protein"
                     /protein_id="HIR1319774.1"
                     /translation="LTTDYVNIESFEGREKQKRAEAFYNARFSSGADFVEQCAHFLVC
                     VIGVIASSVLLYKVNYFMILLILLTCVCEFFLLKILKTKQSETTDNYSKLSGKFEYFY
                     KLSKNAEASKDIKLYGFSDYLVKTAADFIYQIEHINAKYTKQSAAISGVRALLNLVRE
                     LVAYAYLTYLVLKNRLSVSEFIFCFGIITGFSNWIMNLVFSFMEISRCCTDCALYREF
                     "
BASE COUNT          294 a          219 c          250 g          358 t
ORIGIN      
        1 ctttgagctt agcagcgaag tcgttgttag ggctgttcgg cgaaatttct taaccggcaa
       61 agcagtttac gtgtcggcaa ctaatcgatt ttcaggtgaa atatcggtca ttaccgattc
      121 agagcacata gcagagtaat atctataata cttaatgaaa gaagagaagc aatgaaggat
      181 aatttcaaat atatgctttc caattggctg aaatgggata aaaaaagcct caaattcttt
      241 ttcctttggg taccggcatt agtgttgcag ccgttagtta cggcgtatat accgaaagca
      301 atgattgacg ctattgataa tggcgttacc gttggtgata tgacagtcat cgttgcgctt
      361 ttgagcctcc ttttaacgct gacaatatgg cttgacccgt ttatgaagga gcttgtcaga
      421 ggcggcgcaa gaatagttcg tatgcgctat gccgttgcag cgttccgnnn nnnnnnnctt
      481 acaaccgatt atgtcaatat tgaaagcttt gaaggcagag aaaagcaaaa gcgggccgaa
      541 gcattttata atgcgcgctt ttcctccggt gcggattttg tggagcagtg cgctcatttt
      601 ctcgtttgcg ttatcggcgt tattgcctcc tccgttctcc tttacaaagt caattacttt
      661 atgattttgt tgatactcct tacctgtgta tgtgagttct ttctccttaa aatccttaag
      721 acgaagcaga gcgaaacaac cgataattac tcaaagctgt cgggtaaatt cgagtatttc
      781 tacaagcttt ccaagaacgc cgaagcttca aaggatatta agctgtacgg cttttcggat
      841 tatttggtaa agactgcagc ggattttatt tatcagattg aacacattaa tgcaaaatac
      901 accaagcaga gtgctgcaat cagcggcgtg agagccttgc tgaatcttgt tcgtgagctg
      961 gtcgcttatg catatctcac atacctggtg cttaaaaacc gtctttccgt ttcagaattc
     1021 attttctgct ttggcattat tacgggtttt tctaactgga taatgaatct tgtttttagc
     1081 ttcatggaaa tttctcgttg ctgtacggac tgtgcgttgt accgtgaatt t
//
LOCUS       DBMDVO010000595         1129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59082_length_1129_cov_1.056420, whole genome shotgun sequence.
ACCESSION   DBMDVO010000595 DBMDVO010000000
VERSION     DBMDVO010000595.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59082_length_1129_cov_1.056420"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /locus_tag="ACZPE2_07735"
     CDS             <1..>1129
                     /locus_tag="ACZPE2_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319775.1"
                     /translation="KYSIPDYDTELKYRPVIVGFGPAGMFAGLVLAEKGLKPIILERG
                     KAVDERTADVENFWNGGKFNPESNVQFGEGGAGTFSDGKLTTRIKDNRCSYVTESFVD
                     AGAPEEILYDAKPHIGTDKLKGVVKNIREKIIELGGEVRFESRLTDIIVENGAVSAVE
                     INNAEKLKTENVIIAVGHSARDTFKMLYENNVFLEQKPFAAGVRIEHKQSDINYAQYG
                     DKAESLPAADYMLTYTTKKGRGVYSFCMCPGGYVVAAASEECRLVVNGMSEYARDSEN
                     ANSALLVQVYPEDFGSDSPLAGIEFQRRIEEKAFEAGGKNYYAPVQRYEDFAKGQITK
                     AEGVVKHTYRPNTVFADLNNVLPDFICEALKEAIPNMGKRLK"
BASE COUNT          373 a          185 c          294 g          277 t
ORIGIN      
        1 aaatacagta ttccagatta tgatactgag ctcaagtaca gacctgttat tgtcggattt
       61 ggccctgccg gaatgtttgc cgggcttgtg cttgccgaga aaggcttgaa accgataata
      121 cttgagagag gaaaggctgt tgatgaaaga accgcagatg tagaaaactt ttggaatggc
      181 ggaaaattta atccggaaag caatgttcag tttggtgaag gcggcgccgg aacattctcc
      241 gacggaaagc ttacgacaag aattaaggac aacagatgca gctatgttac ggaaagcttt
      301 gttgatgccg gagcgccgga ggaaatatta tatgatgcaa agccgcatat aggtacggat
      361 aagctaaaag gtgttgttaa aaatatccgt gaaaagataa ttgagcttgg tggtgaagtc
      421 agatttgaaa gcagacttac cgatataatt gttgaaaacg gagctgtttc ggcagttgaa
      481 ataaataacg ctgaaaagct taagacagaa aatgttataa tagcggtagg tcacagtgca
      541 agagatactt tcaaaatgct ttatgaaaac aatgtatttt tggagcagaa gccatttgcc
      601 gccggagtaa gaatagaaca caaacagtct gatataaact atgcacaata tggcgacaaa
      661 gccgaaagcc ttcctgcggc tgattatatg cttacatata cgacaaagaa gggcagagga
      721 gtatattcgt tttgtatgtg tcccggcgga tatgttgttg cggctgcatc ggaagaatgc
      781 agacttgttg taaacggaat gagtgaatat gcccgtgaca gcgaaaacgc aaacagtgca
      841 ttgcttgtgc aggtatatcc ggaagatttc ggctcggaca gtccgcttgc cggaattgag
      901 tttcagagaa gaatagaaga aaaggcattt gaagccggtg gaaaaaatta ttatgcacct
      961 gttcagagat atgaggattt cgcaaaagga cagataacaa aggccgaagg tgtcgttaaa
     1021 catacttaca gaccgaatac ggtttttgcc gatctgaata atgtattgcc ggactttata
     1081 tgcgaggcat tgaaagaggc tatacccaat atgggaaaaa ggctaaagg
//
LOCUS       DBMDVO010000596         1128 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59148_length_1128_cov_1.076923, whole genome shotgun sequence.
ACCESSION   DBMDVO010000596 DBMDVO010000000
VERSION     DBMDVO010000596.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59148_length_1128_cov_1.076923"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="ACZPE2_07740"
     CDS             complement(<1..229)
                     /locus_tag="ACZPE2_07740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1319776.1"
                     /translation="MTNETKPDFSEWLVASDIDGTLNNKFRKLPQRNYDAIKDFVVNK
                     KGHFTLASGRNVATMRGPFMGLPITSTPAVIL"
     gene            complement(219..>1128)
                     /locus_tag="ACZPE2_07745"
     CDS             complement(219..>1128)
                     /locus_tag="ACZPE2_07745"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1319777.1"
                     /translation="FADSEFDGVRIDKYLSVVLPDLSRSFIQKAIDCGNVSVNGKTVA
                     KNYKLKTDERIEYIPLEPVKLEVKAQNIPLDIVYEDDDLLVVNKPKGMVVHPAPGNYE
                     NTLVNALLFHCKDSLSGINGVLRPGIVHRIDKDTSGLLIVAKNDFSHRALAEQIKAHS
                     FTREYRAVTIGHLKESSGRVVAPIGRNPKDRKKMAVTDKNSKNAVTNYEVLREYRGYS
                     LLKLRLETGRTHQIRVHMAYLGHPLAGDFVYGTPRTKEELALNGQCLHAGLIGFIHPR
                     TNEYVEFTSDLPLYFKKFIGGLTVDE"
BASE COUNT          324 a          259 c          195 g          350 t
ORIGIN      
        1 ttaatataac agccggtgtt gatgtaatcg gcagtcccat aaaagggcct cgcattgttg
       61 caacatttct gccggacgcc aaagtgaaat gccccttttt attgacaaca aaatctttta
      121 ttgcatcata atttcgctgc ggaagttttc tgaatttatt attaagggtc ccgtcaatat
      181 cagaagctac aagccactct gaaaaatcgg gtttagtttc attcgtcaac agtcaagccc
      241 cctataaact ttttaaagta caatggcaaa tcagatgtaa actccacata ttcatttgtt
      301 ctcggatgaa taaaaccgat aagcccggcg tgcaggcatt gtccgttaag agcaagctct
      361 tcttttgttc tcggagttcc ataaacaaaa tcgcctgcta acggatggcc taaataagcc
      421 atatgtacgc gtatctggtg cgtcctacct gtttcaagcc taagctttaa cagagaatat
      481 cccctgtatt ctcgcaatac ctcataatta gtgacggcat tctttgaatt cttatcagtt
      541 accgccattt ttttgcgatc cttcggattt cttccgattg gtgccacaac tctgccggac
      601 gactctttta aatggcctat tgtaacagct ctatactcac gcgtaaaact gtgagcctta
      661 atttgctccg ccaaagccct gtgagaaaaa tcattttttg caactataag caatccgctt
      721 gtatctttat cgattctatg aacaataccc ggtcttaaaa cgccgttaat tcccgaaaga
      781 ctgtctttgc aatgaaaaag caaagcattg acaagtgtat tctcataatt ccccggtgcc
      841 ggatgtacaa ccataccttt tggcttgttt accacaagca aatcatcatc ttcatataca
      901 atatcaagag gtatgttttg ggctttaact tcaagcttta caggctctaa cggaatatat
      961 tcaattcttt cgtcggtttt aagcttataa ttcttggcta cagtctttcc gtttacactt
     1021 acattcccac agtcaatggc tttttgaata aaagagcgag acaaatcggg taacaccacg
     1081 gataagtatt tatcaatacg tacaccgtca aattcactgt cggcaaaa
//
LOCUS       DBMDVO010000597         1126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59284_length_1126_cov_1.400000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000597 DBMDVO010000000
VERSION     DBMDVO010000597.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59284_length_1126_cov_1.400000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(260..>1126)
                     /locus_tag="ACZPE2_07750"
     CDS             complement(260..>1126)
                     /locus_tag="ACZPE2_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1319778.1"
                     /translation="QITHGMAEHSNRYGRFAVELCKNGYAVFICDLLGHGNSVSDDSE
                     LGYFGENGVESLVDDMKQLNDIARREYPGLPVYLFGHSMGSFLSRAYTAKYPDTIDGT
                     IWCGTSGANPAAGLGAALARAIEKRSGDHHRSDFLNSLAFGKYNKRTENETQFDWLSK
                     DKEEVKKYIDDDYCGFVFTANGFETLFSLLKQISGKDWYRAVPNDKPIFLIAGENDPV
                     GEYGKGVNEVFMTLKKTGHSSVEMKLYPGDRHELLNEIDRDAVTKDITDWLNGQTRSL
                     HVENAAEAVSEK"
BASE COUNT          282 a          270 c          243 g          331 t
ORIGIN      
        1 ttttcatttt tgccgcacct attttgccgc ggtataattt tgcacaacgg cagagtattt
       61 gcgaattcat caaatcattt ccgtaaaggc ttttgataaa aagcattctt atacctccgt
      121 cagcacaaaa actatactgt aattttactt tttacgacag aaaaaatcaa taccaaaaac
      181 cgcggaattt caaaattgga tacatcaaca agtatcataa aataaaataa tgccgtttgt
      241 ctaaaatgac aagtgcgtct tacttttcgg aaacggcctc ggcggcattt tcaacgtgca
      301 ggcttcttgt ctgcccgttg agccaatctg tgatgtcttt tgttacggcg tcacggtcaa
      361 tttcattaag cagctcatgt ctgtcgccgg ggtaaagctt catttcgacc gaactatgcc
      421 cggtcttttt caatgtcatg aatacctcgt ttacaccctt accgtattcg ccgacagggt
      481 cgttttcgcc ggcaattaaa aatatcggct tatcattcgg aaccgcgcgg taccagtcct
      541 tgcccgagat ttgcttcaaa agcgaaaaca gagtttcaaa gccgtttgcg gtaaagacaa
      601 agccgcagta gtcgtcatct atgtattttt taacctcttc cttgtccttc gaaagccagt
      661 cgaattgtgt ttcgttttcg gtgcgcttgt tgtattttcc gaaggcgagc gaattgagaa
      721 agtccgaacg atgatggtcg ccgctgcgct tttcaattgc tcttgccaag gctgcaccaa
      781 gacctgcggc agggttggca ccgcttgttc cgcaccatat cgtaccgtct atggtatcgg
      841 gatattttgc agtatatgcg cgtgagagga acgaacccat gctgtgtccg aaaaggtaca
      901 cagggagtcc cggatattcg cgtcttgcta tgtcgtttaa ttgcttcata tcgtcaacaa
      961 ggctttcaac gccgttttca ccgaagtagc cgagttcgct gtcgtcgctg acggaattgc
     1021 cgtgccccaa aaggtcgcat ataaatacgg cataaccgtt tttacaaagc tcaacggcaa
     1081 atcttccgta gcggttagag tgctcagcca ttccatgcgt tatctg
//
LOCUS       DBMDVO010000598         1126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59290_length_1126_cov_1.259512, whole genome shotgun sequence.
ACCESSION   DBMDVO010000598 DBMDVO010000000
VERSION     DBMDVO010000598.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59290_length_1126_cov_1.259512"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..>1126
                     /locus_tag="ACZPE2_07755"
     CDS             54..>1126
                     /locus_tag="ACZPE2_07755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR1319779.1"
                     /translation="MNIIPKPQKITFSGKYVPKGSVNESFDADMGKEEYSLKINRDGV
                     FLKGGSERALHYADITYKQILREDGNMLPECEIRDKPVFSYRGFMVDVCRHFFTVEEI
                     KKIIDAAAMLKFNYFHFHLSDDQGFRAEIKKHPELSLVGGSREGSHFGKKENDDSVYS
                     HFYTRAQLKEIAEYCKERYIEVIPEIDIPGHASAILQAYPELSCNKEQVKAKTSQGIF
                     KELLCAGNPATLKLIYDILDELCDIFPGKYFHLGGDEAPKDKWKKCPKCQSKIRELGL
                     KNEEELHCSLVNRAAEYLEKKGRRVICWNEAANGGILDKNITLAYWLDKAGVSVKRAN
                     EGCPVIIEKFKPYYADYPYGMYPL"
BASE COUNT          349 a          230 c          273 g          274 t
ORIGIN      
        1 ttatagctat tcttattttg aaaacaaaac tcaatgagaa acgggtgaat tttatgaata
       61 taattccgaa accgcaaaaa ataacgtttt ccggcaaata tgtcccgaaa ggcagcgtaa
      121 acgagagttt tgacgccgat atgggaaaag aggaatactc acttaaaata aaccgtgacg
      181 gcgttttctt aaagggcggc agtgagcgcg ccttacacta tgccgatatt acgtataagc
      241 aaattctgcg agaggacggg aatatgcttc ccgaatgtga aataagggat aagcccgtat
      301 tttcttatcg cggctttatg gtggacgtct gtcgtcattt ctttaccgtg gaggaaataa
      361 aaaagataat cgatgcggcg gcaatgctca aattcaatta ttttcatttt catctttccg
      421 acgatcaggg cttcagagcc gaaataaaaa agcacccgga gctttctttg gtaggcggta
      481 gccgcgaggg ctcacatttc ggtaaaaaag aaaatgacga cagcgtttac agtcattttt
      541 atactcgggc acagctgaaa gaaatagccg aatactgtaa agagcgatat atcgaggtta
      601 ttcccgaaat agatattccc ggccacgcaa gcgccatact tcaggcgtat cccgagctat
      661 cgtgcaataa agagcaggtt aaggcaaaaa caagccaggg tatattcaag gagttgctct
      721 gcgccggaaa tcccgcgaca ttaaagctga tttatgacat tttagatgag ctttgtgata
      781 ttttccccgg caagtacttc catctcggcg gcgatgaagc cccgaaggat aagtggaaaa
      841 agtgccccaa atgtcagtca aaaatacgcg aactggggct taaaaacgaa gaggagctgc
      901 actgcagtct tgtgaacaga gccgcagagt atctcgaaaa aaagggcagg cgtgtcattt
      961 gctggaacga agcggcaaac ggcggtattc tcgataaaaa cataacgctt gcatattggc
     1021 ttgataaagc gggcgtctcg gtaaagcgcg caaatgaggg ctgccccgta attatcgaaa
     1081 agtttaagcc gtattatgcc gattatccgt acggtatgta tccgct
//
LOCUS       DBMDVO010000599         1126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59302_length_1126_cov_1.094634, whole genome shotgun sequence.
ACCESSION   DBMDVO010000599 DBMDVO010000000
VERSION     DBMDVO010000599.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59302_length_1126_cov_1.094634"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            264..917
                     /locus_tag="ACZPE2_07760"
     CDS             264..917
                     /locus_tag="ACZPE2_07760"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HIR1319780.1"
                     /translation="MDKEQILTKIQDCGIVAVVRAESTDKAIRITDACIEGGVAAIEL
                     TFTVPHADEVIEELAKRYTPDEIILGAGTVLDATTARIAMLSGAQYIVSPALDLDTLH
                     LCNRYRVPMMPGIMSVREGLLAMENGADILKVFPADLYGPKIIKDIRGPIPYAKMMPT
                     GGVNADNVGEWIKAGAVAVGAGSSLTAGAKTGDYKQITETGKKFVANIKAARAEMSK"
     gene            947..>1126
                     /locus_tag="ACZPE2_07765"
     CDS             947..>1126
                     /locus_tag="ACZPE2_07765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HIR1319781.1"
                     /translation="MEKTVMVETSARHVHVTEETLEILFGKGYQLTKKKDLSQPGQFA
                     CEERVQVIGPKNSFPA"
BASE COUNT          355 a          254 c          271 g          246 t
ORIGIN      
        1 acttttcggc gcccaatctc gcatatcaaa aatatgcttc ggggcatcaa aaagccgtaa
       61 tatacgaaat cttttccctc tgaaatcaag tggtaaaatc ctacgcatat cagggctttt
      121 cggtgcccaa tctcgcatat caaaaatatg cttcggggca tcaaaaaagc cgtaatatac
      181 gaaatctttt tcatctgaaa ataaaggctg tgcgtaagga gcaagactaa attttaaaat
      241 aaatatatcc ggagatgtta ctaatggata aagaacaaat acttacaaaa attcaggact
      301 gcggtatagt tgcggtagtc cgcgccgaga gcacggacaa agcgattcgt attaccgatg
      361 cttgcatcga gggcggagtt gcagccatag agctgacgtt taccgttcca cacgcagacg
      421 aggtcataga ggaacttgca aagcgctaca caccggacga gataatcctc ggcgcaggca
      481 cggtgctcga tgcaacaacc gctcgtatag caatgctgtc gggtgctcag tatatagtaa
      541 gccccgctct tgaccttgac acgcttcatc tgtgcaaccg ttacagagtt ccgatgatgc
      601 cgggaattat gtcggtacgc gagggcttgc ttgccatgga aaacggagcg gatatactaa
      661 aggtatttcc tgccgacctt tacggaccga aaatcataaa ggacattcgc ggacctattc
      721 cctatgccaa aatgatgccc acgggcggtg ttaacgccga caatgtaggc gaatggataa
      781 aagcgggcgc agtggcagta ggtgcaggtt catcgctcac ggcaggagct aagaccggcg
      841 actataaaca gataaccgaa accggcaaga aattcgttgc aaacatcaag gctgcaagag
      901 ccgaaatgag caaataatca aatcttaaat aaaggggtaa attaaaatgg aaaaaaccgt
      961 aatggtggaa acctccgcaa gacacgttca cgttacagaa gaaacgcttg aaatactctt
     1021 cggtaagggc tatcagctta caaagaagaa ggatctctca cagccgggtc agttcgcctg
     1081 tgaagagaga gtccaggtta taggacctaa aaacagcttt ccggct
//
LOCUS       DBMDVO010000600         1124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59491_length_1124_cov_0.510264, whole genome shotgun sequence.
ACCESSION   DBMDVO010000600 DBMDVO010000000
VERSION     DBMDVO010000600.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59491_length_1124_cov_0.510264"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            179..>566
                     /locus_tag="ACZPE2_07770"
     CDS             179..>566
                     /locus_tag="ACZPE2_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319782.1"
                     /translation="MSDYDVLYIDKSVVETGGFNASAVEEYVSNGGSVFLDNDVYNVF
                     DKDFIGAEDFVTINSCPVDMIYPENSDKGIKKIQSLLSDFSMLYRNYADYNDVLVNQN
                     YGVGVVPSTAECIVSKDNVGIYTVNQY"
     assembly_gap    567..576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <577..>1124
                     /locus_tag="ACZPE2_07775"
     CDS             <577..>1124
                     /locus_tag="ACZPE2_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319783.1"
                     /translation="SVNNLSPDDTGEYLSATTVGANKLLRDYFAEFVSLKNYGYAVAH
                     VLGSFAKPVASWELHYEDITGMDNGSAEIFEEMCERYGQVPSFTLARNPYIWFRRAES
                     VTYALNNNGQFAMDPYENAYSSGTHFVTAKEWLSLDYDDNTISYFEDSHDYIKRAYPC
                     PTDFNSDGKMDLICGSADGKLY"
BASE COUNT          326 a          190 c          259 g          339 t
ORIGIN      
        1 gtctttatca ggcactactg aaactaagac ataaagagta ttaattacat tatctgtact
       61 gtctttaatc ttaatttgaa catatgtctg tccgacagtt ttacctgtta ctgtaccgtt
      121 tgaatcaacc gttgcaacat tgaccgccga taaaatcgat gcgtcaaata tcggtgattt
      181 gtcagattat gatgttttgt acattgataa aagcgttgtg gaaacaggcg gttttaatgc
      241 aagtgcagtt gaggaatatg tatcaaacgg cggaagtgtt tttcttgaca atgatgtgta
      301 taacgtattt gataaggact ttatcggtgc ggaggacttt gtaacgatta attcttgtcc
      361 ggttgatatg atttatcccg agaacagcga taaaggaata aagaaaattc agtcgctttt
      421 gtctgatttt tcgatgttgt acagaaatta tgccgattat aatgatgtgc ttgtaaatca
      481 aaattacgga gtcggcgttg ttccgtcaac agcggaatgt attgtgtcaa aggataatgt
      541 gggaatttat acggtaaatc agtacgnnnn nnnnnntccg taaacaatct ttctcccgat
      601 gataccggcg aatacctttc agcaacaacg gtcggtgcaa ataaattgct cagagattat
      661 tttgcggaat ttgtttcgct taaaaattac ggatatgccg ttgcacacgt tttgggcagc
      721 tttgcaaagc ctgttgcctc gtgggagctt cactatgagg atattacggg tatggataac
      781 ggctcggcgg aaatttttga ggaaatgtgt gaaagatacg gtcaagtacc gtcattcacg
      841 cttgcaagaa atccgtatat ttggttcaga cgtgccgaaa gtgtgacgta tgcactgaat
      901 aataacggtc aatttgcaat ggatccgtat gaaaatgcgt attcatcggg aacgcacttt
      961 gtaaccgcaa aggaatggct gtcacttgat tatgacgata acacaatcag ctattttgag
     1021 gacagccacg attatataaa acgtgcttat ccgtgtccga cggactttaa cagtgacggc
     1081 aagatggact tgatttgcgg aagtgcggac ggaaaactgt acta
//
LOCUS       DBMDVO010000601         1123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59513_length_1123_cov_1.449119, whole genome shotgun sequence.
ACCESSION   DBMDVO010000601 DBMDVO010000000
VERSION     DBMDVO010000601.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59513_length_1123_cov_1.449119"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /gene="thrC"
                     /locus_tag="ACZPE2_07780"
     CDS             <1..>1123
                     /gene="thrC"
                     /locus_tag="ACZPE2_07780"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1319784.1"
                     /translation="LDIKYDYKYIASRVTAKSLSESRDYSMWRYREFLPVEEDTKNTP
                     LRVGYSPFYEVKKLAEMLDIGRLWIKDDGINPTASLKDRASAMAVAKAYEAKADTIAC
                     SSTGNAASSLAGNAAAAGFKTYIFVPSRAPKGKVAQLMIFGANVISVQGSYEDTFMIS
                     AEAIDRWGWYNRNAAINPYLVEGKKTVSLEIAEQLGFKMPDYVAVSVGDGCTIAGVWK
                     GFKDLYETGIIDRLPRLISVQAAGCCPINRAIESNSPLQPMEENTIADSIAVGVPRNP
                     DKAMNAIRESNGIVVNVTDEEILGAMRLLGRTCGLFGEPAGVTGAAGIKKARQLNLIP
                     ADATVVGIISGNGLKDVNNAIKAAGEPISIPPKMDDLLNA"
BASE COUNT          323 a          256 c          267 g          277 t
ORIGIN      
        1 cttgatatta aatacgatta taaatacatt gcatcaagag ttacggcaaa atcactttcc
       61 gaaagccgtg actactcaat gtggcgatac agagaatttc ttcccgttga ggaggataca
      121 aaaaatacac ctttaagagt aggctattca ccgttttatg aggttaaaaa gcttgccgaa
      181 atgctcgaca tcggcagact ttggataaag gacgatggaa tcaatcctac cgcaagcctt
      241 aaggacagag cctccgcaat ggctgttgcc aaggcatatg aggcaaaggc agatactatc
      301 gcctgctcat caacaggcaa tgcggcatca tctcttgccg gcaatgctgc tgccgcaggt
      361 tttaaaacat atattttcgt tccctcgaga gcacctaagg gcaaggttgc acagcttatg
      421 attttcggtg caaacgtaat ctccgttcag ggttcatatg aagatacatt tatgatttcc
      481 gcagaggcta ttgaccgttg gggctggtac aacagaaatg cggcaatcaa cccgtatctt
      541 gtggaaggca aaaagaccgt ttctctcgaa atagccgaac agctcggttt taaaatgccg
      601 gactatgttg ctgtttccgt aggcgacggc tgtacaatcg ccggcgtttg gaagggcttt
      661 aaagaccttt acgaaacagg cattatcgac agacttccaa gacttatcag tgttcaggcg
      721 gccggctgtt gccctataaa cagagccatt gagtcaaact caccgcttca gcctatggag
      781 gaaaacacta tcgccgattc aatagcagtc ggtgttccca gaaatcccga taaggcaatg
      841 aacgcaataa gagaatccaa cggcattgtt gtaaacgtaa cagatgagga aatactcgga
      901 gctatgcgtc ttttgggccg tacttgcggt cttttcggcg agcctgccgg tgtgacggga
      961 gctgccggaa taaagaaggc aagacagctt aaccttattc ctgccgatgc aactgttgtc
     1021 ggaattatat ccggtaacgg tcttaaggac gtaaacaatg cgataaaagc cgccggagag
     1081 cctatatcaa ttccgcctaa aatggacgat cttcttaacg ctt
//
LOCUS       DBMDVO010000602         1120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59745_length_1120_cov_1.120707, whole genome shotgun sequence.
ACCESSION   DBMDVO010000602 DBMDVO010000000
VERSION     DBMDVO010000602.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59745_length_1120_cov_1.120707"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(164..901)
                     /locus_tag="ACZPE2_07785"
     CDS             complement(164..901)
                     /locus_tag="ACZPE2_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1319785.1"
                     /translation="MIKTKMAERLKSNQMSDTFTNAIFLTLSGGFQDAYTYLCRGKVF
                     ANAQTGNIVLMSTHFFEGEFGLILHYLIPVISFIFGICVAEIQHIFFKNYKKIHWRQI
                     VLAFEIVILLTVGFIPINLNILANALVSFVAAMQVQTFRKLRGNAYASTMCIGNMRSG
                     AEALCGYFKTRKKILLIKAIEYFSIIFMFAVGAGFGSLAADRWGIRSIWFSCAFLFIS
                     FIIMFIRLEIEEEKEEELEKAVENEFK"
     gene            1026..>1120
                     /locus_tag="ACZPE2_07790"
     CDS             1026..>1120
                     /locus_tag="ACZPE2_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1319786.1"
                     /translation="MLVNYEYYKIFYCVAKYQNFTKAAVELDNNQP"
BASE COUNT          373 a          205 c          188 g          354 t
ORIGIN      
        1 tgctctgaat cacatacttg acaatgtgct tcttgattgc aacacatgca atttaaaatc
       61 aatttgcgac gaggttgagg gtatgaaaga actgcatatg aaaaacgctg caaagaaaca
      121 gtaaaaaagc aggcaaggcc taaaagcttt gcctgcttaa attttatttg aattcgtttt
      181 caacagcctt ttcaagttct tcttcttttt cttcttcaat ttcaaggcgg ataaacataa
      241 tgataaagct tataaacaaa aatgcacatg aaaaccaaat cgagcgtata ccccatctgt
      301 ctgccgcaag actaccgaat ccggcaccta cagcaaacat gaatataatg ctgaaatatt
      361 caattgcctt tataagcagt atttttttac gtgttttaaa atagccgcac aaagcttccg
      421 cgccgcttct catattgccg atgcacatcg tgcttgcata ggcgtttccg cgcaacttgc
      481 ggaatgtttg aacctgcatc gccgctacaa aagaaacaag agcattagcg agtatgttta
      541 agttaatcgg gataaaaccg acggtcaaaa gtataactat ttcaaatgct aaaactattt
      601 ggcgccaatg tatctttttg taatttttaa agaaaatatg ctgtatttcg gcaacgcata
      661 tcccgaatat aaaagaaatt accggtataa gataatgtaa tattaacccg aattctccct
      721 cgaaaaaatg tgtgctcata agcacaatat ttccggtttg agcgtttgcg aaaacctttc
      781 cgcggcaaag atatgtataa gcgtcctgaa atcctccgga aagagtcaga aaaattgcat
      841 ttgtaaaggt gtctgacatt tgattagatt ttaatctttc tgccattttg gttttaatca
      901 tattttacct gtcctctaac aaagtttgac aatcccaatc tttattatta taatacatac
      961 atatctatat ttaaaatata ttgttgatat ggctaatatg taatttttat attgagagga
     1021 attaaatgtt ggtaaattac gaatattaca aaatttttta ttgcgttgcg aaatatcaga
     1081 atttcacaaa agctgcggtt gagcttgata ataatcagcc
//
LOCUS       DBMDVO010000603         1118 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59887_length_1118_cov_1.007866, whole genome shotgun sequence.
ACCESSION   DBMDVO010000603 DBMDVO010000000
VERSION     DBMDVO010000603.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59887_length_1118_cov_1.007866"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            73..>1118
                     /locus_tag="ACZPE2_07795"
     CDS             73..>1118
                     /locus_tag="ACZPE2_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR1319787.1"
                     /translation="MRGTKKFIGIIISLSILFLCFFSFGADAADGFSVFSTGATAHTG
                     ETVTISIKNTEASLGSYSVYVNYDKTKLQYKSSSLGDAGSALPQASCTHIESDSQVRY
                     AGATSSQNNVQVKAGTVMTITFLVIGNTSGSTPVGTPISVYAAAYDSSAKSISVTSQG
                     NSVLIMPSSGNNSGQQVQNNATEAPVQQNNSGDSSVSSSDATLKSMRVYGITDAGETT
                     VDITLSPEFSSDTLFYNASVMSDVARIQVDAVPNNEKAQVSIPMGYLKMDIGSNTTKI
                     TVTAEDGVTTMTYILTTVKSESGTSIIAPVETEPYTEGLSDEPMTEVTAAEPETQNNA
                     IASSADNIKNAVKN"
BASE COUNT          321 a          252 c          261 g          284 t
ORIGIN      
        1 cgttgggtag tacggcttgt gtattatccg aatgtttgca aatttgttaa tatgattctt
       61 tcggaggacc ctatgagagg cactaaaaaa tttatcggta ttataatttc gctttcgata
      121 ctttttttgt gctttttttc tttcggcgcc gatgcggcgg acgggttctc cgttttttca
      181 accggagcca cggcgcatac aggcgagaca gtcacaataa gcattaaaaa taccgaagcc
      241 tccctcggct cgtattccgt gtatgtaaat tatgacaaaa ccaagctgca gtataagtca
      301 agctcactcg gtgatgccgg cagcgcgttg ccgcaggctt catgcacaca tatcgaaagt
      361 gattctcagg tgcgatatgc cggtgcaacc tcctcacaga acaatgtgca ggtaaaggca
      421 ggcaccgtta tgacgataac ttttcttgtt atcggaaata catcgggctc tactcccgta
      481 ggtacaccta tttcagtgta cgcggcagcc tatgattcaa gtgctaaaag cataagtgtt
      541 acgtctcaag gcaattccgt gctcataatg ccatcatccg gtaataattc cggtcagcag
      601 gtgcagaaca acgcaacgga agctcccgta cagcaaaata acagcggcga tagctccgta
      661 agctcaagtg atgcaacgct taaatcaatg cgtgtttacg gtataactga tgcaggcgaa
      721 acaacagtag acattacgct ttctccggag ttttcgtcgg atacgctttt ttataatgcg
      781 agcgtaatga gcgacgtagc gagaattcag gtagacgccg tgccgaataa tgaaaaggca
      841 caggtaagta tacctatggg ctatcttaaa atggatatag gaagcaacac cacaaagatt
      901 accgttaccg cagaggacgg cgttacaact atgacgtata ttttgactac ggttaaatcc
      961 gagtcgggta cgtcgataat agcacctgtt gagacagaac cttataccga ggggctttcc
     1021 gatgagccga tgacggaagt aaccgccgca gagccggaaa cacaaaataa cgcgatagct
     1081 tcttctgctg ataatatcaa aaatgccgtt aaaaacca
//
LOCUS       DBMDVO010000604         1117 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59931_length_1117_cov_1.362205, whole genome shotgun sequence.
ACCESSION   DBMDVO010000604 DBMDVO010000000
VERSION     DBMDVO010000604.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59931_length_1117_cov_1.362205"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="ACZPE2_07800"
     CDS             complement(<1..>1117)
                     /locus_tag="ACZPE2_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1319788.1"
                     /translation="AWKESEHRKPLILQGARQVGKTYSILEFGRTHYENVAYFNFETN
                     PKLNETFEENISPDYLIPILSHIAGQTIVKEKTLIVFDEVQLCERALTSLKYFCENAP
                     DYHIIAAGSLLGVAASREKFSFPVGKVDMKTLYPMDMEEFMLALGEDDLVEQIKKCFQ
                     TDTPLPSALHDAAMQLYRQYLVVGGMPECVMQFVETKDYILVRHTQDTILASYLNDMS
                     KYNNLNEIKKTRLAYDNITVQLSKKNTRFQYKLIKKGGRASEFENAITWLCLSGIVSQ
                     VYKVEQIKKPLENYRDIDAFKIYVSDLGLLCAKKDLAANDVLYMVEEINDFKGGMAEN
                     YVNVQLTMNGYHTYYWESARGAEIDFIIQRNGQLIPI"
BASE COUNT          284 a          258 c          243 g          332 t
ORIGIN      
        1 tcaatgggaa tgagctgacc gttacgctga ataataaaat caatttcagc tcctcgcgcg
       61 gactcccaat agtaggtgtg gtagccgttc atggtaagct gcacattgac ataattctct
      121 gccataccgc ccttgaagtc gttaatttct tcgaccatat agaggacgtc attggcggct
      181 aaatcttttt tggcgcaaag caatcctaag tcggacacat aaatcttgaa cgcatcaata
      241 tcgcggtagt tttcaagcgg ctttttgatt tgctcgacct tgtagacctg tgatacgatg
      301 ccggacagac aaagccatgt aatcgcattt tcaaattcgg aagcccgtcc gccttttttt
      361 atcagtttat attggaaacg ggtattcttt ttggagagtt gaacggtaat gttgtcgtag
      421 gcaagtctgg ttttcttgat ttcatttaag ttattatact tgctcatatc gttgagatag
      481 cttgcaagta tcgtatcctg cgtatggcgt acaaggatat agtcctttgt ttcaacgaat
      541 tgcatcacgc actccggcat accgccgact acaagatatt gacggtaaag ctgcatggct
      601 gcgtcatgca aggcagacgg caaaggcgta tcagtttgga agcacttttt aatctgctct
      661 accaaatcgt cctcgccgag cgccagcata aattcctcca tatccatagg ataaagcgtt
      721 ttcatatcga cctttcctac aggaaaagag aatttctctc tgctcgccgc aacgccgagc
      781 aagctgcctg ccgcgatgat gtgataatcc ggagcattct cgcaaaaata tttgagcgaa
      841 gtcaaagctc tttcgcagag ctgcacctcg tcgaatacaa tcagcgtttt ttccttcacg
      901 atggtctgcc ctgcgatatg ggacaaaatc ggtatcagat aatcggggct gatgttttcc
      961 tcaaaggttt cgttcagctt tggattcgtt tcaaagttaa agtatgccac attttcgtaa
     1021 tgggtgcgtc caaactccaa aatggaatag gtctttccaa cctgtcttgc accctgcaaa
     1081 ataaggggct tacggtgttc actttctttc cacgctt
//
LOCUS       DBMDVO010000605         1117 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_59941_length_1117_cov_1.187992, whole genome shotgun sequence.
ACCESSION   DBMDVO010000605 DBMDVO010000000
VERSION     DBMDVO010000605.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59941_length_1117_cov_1.187992"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(121..>1117)
                     /locus_tag="ACZPE2_07805"
     CDS             complement(121..>1117)
                     /locus_tag="ACZPE2_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1319789.1"
                     /translation="RADLEVCYCGDIAAAVGVKNTTTGDTLCDENNPVILESMEFPEP
                     VISLAIEPKTKAGQEKMAIALAKLAEEDPTFRTYTNEETGQTIIAGMGELHLEIIVDR
                     LLREFKVEANVGAPQVAYKETITTNADVDTKYARQSGGKGQYGHVKLHVYPNESGKGY
                     EFINAIVGGSIPKEYIPAVDAGIQGALQSGVLAGYQTVDVKVELYDGSYHEVDSSEMA
                     FKIAGSMAIKEACRKANPVILEPIMKVVVTVPEEYMGDVIGDLSSRRGEIQGMEDRNG
                     VKQINAFVPLSEMFGYSNDLRSKSQGRGQYVMEPSHYIQVPKSIADGIISKRAKD"
BASE COUNT          277 a          269 c          236 g          335 t
ORIGIN      
        1 caaatttctg ctttgccatt ggaattttcc tcctttaatt atgtgtattc catacactta
       61 gtattttatc agattatttc tcaaaaaaca agtctttttt gctctttcag aaaaatttta
      121 ttagtcctta gctctcttgc tgatgattcc atctgcaatt gacttgggaa cctgaatgta
      181 gtggctgggt tccattacat actgaccgcg accctgtgac ttagaacgca gatcgtttga
      241 gtaaccgaac atttctgaca gaggaacgaa agcattgatc tgcttaacgc cgtttctgtc
      301 ttccataccc tgtatctcac cacgacgtga gcttaaatcg ccgataacgt cgcccatgta
      361 ttcttcggga actgttacaa ctaccttcat gataggttca aggataacgg ggttagcctt
      421 gcggcatgct tccttgatag ccattgaacc tgcgatcttg aatgccattt cagaagagtc
      481 gacctcgtgg tatgaaccgt cataaagctc aaccttaacg tctactgtct gatagcctgc
      541 aagaacaccg gactgtaaag caccctgaat acctgcgtca actgcgggga tatattcctt
      601 aggaatagaa ccgccgacaa ttgcgttaat gaactcatag cccttaccgg actcgttcgg
      661 gtaaacgtgg agcttaacgt gaccgtactg acccttacca cctgactgac gtgcatactt
      721 tgtatcaacg tctgcattag tggtgattgt ctctttatat gcaacctgag gagcaccgac
      781 attagcctca actttaaatt cacggagaag tctgtctacg atgatttcga ggtgaagctc
      841 gcccattccg gcgatgattg tctgacctgt ttcctcattt gtataggttc tgaaagtggg
      901 gtcttcctct gcaagctttg caagagcaat agccatcttt tcctgacctg ccttggtctt
      961 gggttcgata gcgagcgaga taacaggctc ggggaattcc atagactcaa ggattacggg
     1021 attgttttca tcacacagcg tatcacctgt tgtagtgttc ttaacaccta cagcagctgc
     1081 aatatcgccg cagtagcaaa cttcgaggtc tgctctg
//
LOCUS       DBMDVO010000606         1116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60016_length_1116_cov_1.172414, whole genome shotgun sequence.
ACCESSION   DBMDVO010000606 DBMDVO010000000
VERSION     DBMDVO010000606.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60016_length_1116_cov_1.172414"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="ACZPE2_07810"
     CDS             complement(<1..349)
                     /locus_tag="ACZPE2_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR1319790.1"
                     /translation="MENVNIKINGMPLSVPNGISILEAARYAGIEIPTLCFLKDINEI
                     GACRICMVEVKGARNLVAACVFPVAEGMEIFTNTEKVRRSRKTTLELILSTHDKKCLT
                     CVRSGNCELQKLCK"
     gene            complement(362..>1116)
                     /locus_tag="ACZPE2_07815"
     CDS             complement(362..>1116)
                     /locus_tag="ACZPE2_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR1319791.1"
                     /translation="SKGTKVFAVNGKINNTGLVEVPMGTTLRTIIEDIGGGIPNGKKF
                     KAAQTGGPSGGCIPASLMDTPIDYDNLTAIGCMMGSGGLIVMDEDDCMVDIAKFFLNF
                     TVDESCGKCTPCRVGTKRLLEMLEKITSGNATLRDLDKLEELCHYIKANSLCGLGQTA
                     PNPVLATLKFFRDEYVAHVADKKCPAGVCKALLSYEILEDRCRGCTACARKCPVGAIS
                     GNVKEPHVINKSLCVKCGVCMQTCKFGAIVKR"
BASE COUNT          267 a          293 c          245 g          311 t
ORIGIN      
        1 ccttgcagag cttttgcagc tcacagtttc cgctgcgcac acaagttaaa cattttttat
       61 catgagttga aagtataagc tcaagagtgg tctttctcga acgtctgacc ttctcggtat
      121 tggtgaatat ttccataccc tcggccacgg ggaaaacaca cgccgcaaca aggtttcttg
      181 cgcccttaac ctctaccata catatgcggc aggcgcctat ctcgtttata tcctttaaga
      241 aacaaagcgt cgggatttca attccggcgt atctcgccgc ctcaagtatt gaaattccgt
      301 ttggcacact caggggcatg ccgttaattt ttatattgac attttccatt tggcacactc
      361 cttatctttt aactattgcg ccgaatttgc aggtctgcat acaaactccg cacttgacgc
      421 aaagagattt atttataacg tgaggctctt ttacatttcc gcttattgcc cctacgggac
      481 attttctcgc acaggcggtg cagccgcggc atctgtcctc aagtatctca taagacaaaa
      541 gcgccttaca tacgcctgcc ggacatttct tgtcagccac gtgagccaca tactcgtcac
      601 ggaagaattt aagagtggcg agcaccggat tcggagccgt ctggccgagt ccgcagaggg
      661 aatttgcctt gatgtaatgg cagagctcct caagcttgtc aaggtcccta agcgttgcgt
      721 tgcccgatgt tatcttttcg agcatttcaa gcaggcgttt tgtgccgacg cgacacggcg
      781 tacatttgcc gcatgattcg tctacggtaa agttaaggaa gaatttggca atatccacca
      841 tacagtcgtc ctcatccatt actatgagtc ctccggagcc catcatacag cctatcgccg
      901 taagattgtc atagtcaatg ggagtatcca taagcgaagc cggtatgcat ccgccggagg
      961 ggccgcctgt ctgagcagcc ttaaacttct tgccgtttgg aataccgcca ccgatatcct
     1021 cgatgatggt acgaagtgtc gtacccatag gaacttctac aagacctgta ttgttaatct
     1081 taccattgac agcgaatacc tttgtacctt tggact
//
LOCUS       DBMDVO010000607         1116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60040_length_1116_cov_0.839409, whole genome shotgun sequence.
ACCESSION   DBMDVO010000607 DBMDVO010000000
VERSION     DBMDVO010000607.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60040_length_1116_cov_0.839409"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /gene="rsmG"
                     /locus_tag="ACZPE2_07820"
     CDS             <1..537
                     /gene="rsmG"
                     /locus_tag="ACZPE2_07820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1319792.1"
                     /translation="HFIDSVLPFSMIDIKENSSFIDVGTGAGFPSIPLMIVRPDLKGI
                     LLEALNKRCVFLEKACELTGVDAKVIHGRAEDYAKEKREAFDFATARAVAAMPVLCEY
                     CIPYVKTGGRFIALKSVNEDETLCEKAVNVLGGKIAEIKDYTITNGDNRRLFIIDKVS
                     QTPTKYPRNPSMIKKKPL"
     gene            644..>1116
                     /locus_tag="ACZPE2_07825"
     CDS             644..>1116
                     /locus_tag="ACZPE2_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1319793.1"
                     /translation="MGLFTKDKKITGQVITVNVREIDLNPNQPRKYFDKAELESLAIS
                     IKENGLLQPLTVRKKSGGRYELIAGERRLRACILAGINEVRVIIEDRNEEESSVLAII
                     ENIQRCDLNCIEEAVAMQRLMDHYGYTQEELARKLGKAQSTIANKIRLLKLSDKL"
BASE COUNT          390 a          173 c          278 g          275 t
ORIGIN      
        1 catttcatag acagcgtgtt gccgttcagt atgattgaca ttaaagaaaa cagcagtttt
       61 attgatgtcg gcacaggagc gggatttccg tccattccgc ttatgatagt aagaccggat
      121 cttaaaggca tactgcttga ggcactgaat aagagatgtg tattccttga aaaagcgtgc
      181 gagcttaccg gagttgatgc gaaggtgatt cacggcagag cagaggacta cgcaaaagaa
      241 aagcgtgaag cgtttgattt tgcaacagca agggctgttg cggctatgcc ggtactttgt
      301 gagtattgta taccttatgt aaaaacgggc ggcaggttca ttgctcttaa gtctgtaaat
      361 gaggatgaaa ccctgtgcga aaaggcagtc aacgtgcttg gaggaaagat agctgaaata
      421 aaagactata caataacaaa tggcgacaac agacgactat ttattataga taaagtaagt
      481 cagactccga caaaatatcc gagaaatcct tcgatgatta agaagaaacc actgtaatac
      541 agtgaaaata taaaacattc cgtcgttttt agcagataaa cggcggattt tttttattgc
      601 ccgaggaata agtttgatat aaaattaggt tgggggtgaa ataatggggt tatttactaa
      661 ggataagaaa attacgggac aagttattac agtgaatgtc agagaaatag acttaaaccc
      721 aaatcagccg agaaagtatt ttgataaagc agaattagaa agccttgcga taagcataaa
      781 agagaacggt ctgttacagc cgcttacagt gagaaaaaaa tctggcggca gatatgagct
      841 tatagcggga gaaaggcggc taagagcatg catacttgcc ggtataaatg aggtcagggt
      901 aattattgag gacagaaatg aagaagaatc ttcggtgctt gcaataatag aaaacataca
      961 aaggtgcgat cttaattgca ttgaggaagc tgttgcaatg caaaggctaa tggatcatta
     1021 cgggtatact caggaagaac ttgcaagaaa gctcggaaaa gcgcagagta cgatagcaaa
     1081 taagataagg cttctgaagt tatcagacaa gctgtt
//
LOCUS       DBMDVO010000608         1115 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60096_length_1115_cov_1.138067, whole genome shotgun sequence.
ACCESSION   DBMDVO010000608 DBMDVO010000000
VERSION     DBMDVO010000608.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60096_length_1115_cov_1.138067"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="ACZPE2_07830"
     CDS             complement(<1..312)
                     /locus_tag="ACZPE2_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319794.1"
                     /translation="MKYSKEVYEKTKSVLDSRRIKAETDAEKRRVEFAKVHPELLVIE
                     DELTKTGLEAVKAVIGGSGDVKKTVEELMKTNLQLQKDRENLLLSAGVDKNYLKPHYT
                     CE"
     gene            complement(313..>1115)
                     /locus_tag="ACZPE2_07835"
     CDS             complement(313..>1115)
                     /locus_tag="ACZPE2_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1319795.1"
                     /translation="NGTAFTSETVEPVKPDDSGVNAQNKSEPMASCVAVPEKRPLPEI
                     KIAKPTLEQISKRMEESGDVLRLMSESQSILGRTIGFDMQSTLLMLYDTYGLSVEVIF
                     MLLEFCVHQSRSSTAYVAKLGKVWAEKEINTVELAADYIEKSTEAEKTFSVLRELTGM
                     STPRPTQKQTEYILSWRALGFEAPIIARAYDEMAERTGKISFNYMDKILLNWHTAGYK
                     TPEDVENGERLFREKKQAVKQKGTSFSIDEAISDAGMGVVKYKKKGVK"
BASE COUNT          247 a          296 c          229 g          343 t
ORIGIN      
        1 ttcacaggta taatgaggtt ttagataatt tttatctacc cccgcgctca gaagcaggtt
       61 ttctcgatcc ttttgaagct gcaaattagt cttcataagc tcttcgaccg tctttttaac
      121 gtcgccgctt ccgccgataa cggcttttac ggcttcaagc ccggttttgg tcaattcatc
      181 ctctatcaca agcaattccg gatggacttt cgcaaactca acgcgccttt tttccgcatc
      241 ggtttccgct tttatacggc gtgagtcaag aacggatttt gtcttttcat aaacttcctt
      301 tgaatacttc aatcacttca cgcctttctt tttatatttc acaacgccca ttcctgcatc
      361 ggatattgcc tcatctatgc tgaatgacgt ccccttttgc tttaccgcct gtttcttttc
      421 acggaaaaga cgttcgccgt tttccacgtc ctccggcgtt ttgtacccgg ccgtatgcca
      481 attcaggaga atcttatcca tataattaaa cgatatttta ccggtgcgct cagccatctc
      541 gtcatatgct cttgcgatta tcggagcttc aaacccgaga gcgcgccacg acaaaatgta
      601 ttccgtctgc ttttgagtgg gccgcggagt tgacataccc gtaagctcac gcagaacgga
      661 aaaggttttt tcggcttcgg tgctcttttc tatgtaatcg gcggcaagct ccaccgtgtt
      721 tatttctttt tccgcccaaa ccttgccgag ctttgcaaca taagccgttg acgagcgcga
      781 ctgatgcaca caaaattcaa gcagcataaa tataacttcg acgctgaggc cgtatgtatc
      841 ataaagcatc aaaagcgtgc tctgcatatc aaatcctatt gttctgccga gaatgctctg
      901 cgattcggac ataagacgaa gaacgtcccc gctttcctcc atacgctttg aaatttgctc
      961 gagcgtgggc tttgcaattt taatttcggg cagtggccgc ttctcgggaa cggcaacaca
     1021 cgaagccatc ggttccgatt tgttttgcgc atttacgccg ctgtcgtcgg gctttaccgg
     1081 ctcaactgtt tcggaagtaa aagcggtacc gtttt
//
LOCUS       DBMDVO010000609         1114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60184_length_1114_cov_0.995064, whole genome shotgun sequence.
ACCESSION   DBMDVO010000609 DBMDVO010000000
VERSION     DBMDVO010000609.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60184_length_1114_cov_0.995064"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="ACZPE2_07840"
     CDS             <1..679
                     /locus_tag="ACZPE2_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319796.1"
                     /translation="PTDPSGTTNPSGTTNPSGTTNPSGTTNPGGDHEDPCVRMGMKGN
                     VYRGKNLFPGAKLDIDLGEIKAEAGKDGAYSVDVIKDGFHVVTITDENGEVAGKTTFS
                     VSVSDTATETTVIKLDDGSQIAVVPKGTNTLWLDFVVNDDGTITIVPGTPVEEPTSEV
                     PVTKPSDSGNPIMNNPVISKTGELIRENPLAAAAVFLCGLSLIFFIILRKKRKDDEEE
                     ENTKPVC"
     gene            837..>1114
                     /locus_tag="ACZPE2_07845"
     CDS             837..>1114
                     /locus_tag="ACZPE2_07845"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine
                     O-acetyltransferase/O-succinyltransferase family protein"
                     /protein_id="HIR1319797.1"
                     /translation="MPIKIDNQLPAHHSLELENIFVMTEDRAVKQDIRPLKIIILNLM
                     PTKIETETQLLRLLSNSPLQLEIDLLQVKSHISKNVSREHMLKFYKTY"
BASE COUNT          342 a          249 c          247 g          276 t
ORIGIN      
        1 tcctacagat ccctcaggca caacaaatcc ctcaggtact accaatcctt cgggcaccac
       61 taatccctcg ggcactacaa atcccggcgg agatcacgaa gatccttgtg ttcgtatggg
      121 catgaagggt aatgtgtatc gcggtaaaaa ccttttcccg ggcgctaagc tcgacattga
      181 cctcggcgaa atcaaggctg aggcaggaaa agacggtgca tacagcgttg acgttattaa
      241 agacggtttc catgttgtaa caatcaccga cgaaaacggt gaagtagccg gcaagactac
      301 tttctcggta agcgtaagtg atacggcaac agaaacaacc gttattaaac ttgatgacgg
      361 ctcgcagatt gcagttgttc cgaagggcac caatacctta tggctcgact ttgtcgttaa
      421 cgacgacggt actattacta ttgttcccgg cacacccgta gaggagccga caagcgaagt
      481 tccggtaacc aaaccgagcg acagcggcaa cccgataatg aataacccgg taatatccaa
      541 aaccggtgaa cttatccgtg aaaatccgct cgccgcagct gctgttttcc tttgtggatt
      601 gtcacttata ttcttcataa ttcttcgtaa aaaacgtaag gacgacgagg aagaggaaaa
      661 cacaaaaccc gtttgctgat ataaacggta ataaataagt ttaaaatacc ccgactgcgg
      721 tcggggtatt tttatgtgta tcggcattgc aaacgatgat gtgttatgtt ataatacttt
      781 gtcagaactt aaacgggtaa aagaccgagg ttcgtcttct tgaaagaggt ggctgtatgc
      841 ccataaaaat agataatcag cttccggcgc atcatagtct ggagcttgaa aatatatttg
      901 ttatgacgga ggacagagcc gtcaagcagg acattcgtcc gcttaaaatt ataatactga
      961 atttaatgcc tacaaaaatc gagaccgaaa ctcagctttt aaggcttctc agtaattctc
     1021 cgcttcagct tgaaatagac ttgctgcagg taaaaagcca tatttcaaaa aatgtgtcaa
     1081 gggagcatat gctcaaattc tacaaaacct atga
//
LOCUS       DBMDVO010000610         1113 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60250_length_1113_cov_1.085968, whole genome shotgun sequence.
ACCESSION   DBMDVO010000610 DBMDVO010000000
VERSION     DBMDVO010000610.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60250_length_1113_cov_1.085968"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..573)
                     /locus_tag="ACZPE2_07850"
     CDS             complement(31..573)
                     /locus_tag="ACZPE2_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014615.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1319798.1"
                     /translation="MKFIDLLGKERLFFDGAMGTMLQKNGLSAGEIPETWNITHRDTV
                     YAIHKAYADAGCNIIKSNTFGANALKFKGTDYTVEEIVTSAIDIAKTAVSGKDKTFVA
                     LDLGPTGKLLKPYGELPFETAYELYKQQVIAGKKAGAGALRSQKEGGAGPSGSGAAGT
                     AGFGYAQGAVPGGVRRKEIR"
     gene            complement(577..>1113)
                     /locus_tag="ACZPE2_07855"
     CDS             complement(577..>1113)
                     /locus_tag="ACZPE2_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014961.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR1319799.1"
                     /translation="TSIQPRSLYRECKLEYLSDSAVKLDGIEFHSEKLVKHLRNSDRI
                     LLFAATLGMQSDILVRRYAGVDTARAAVMQAATAAAVESFCDDVCENIAKEEEKRGYY
                     LRPRFSPGYADLSLESQKEFFKLLDCTKRIGLTLSENCMMIPTKSVTAFIGLTKDKDC
                     NFNACAACGNKNCEFRRG"
BASE COUNT          253 a          309 c          237 g          314 t
ORIGIN      
        1 ataaggtact gcacctcgtc catgccggtc tcatctaatc tcttttctcc gaactccacc
       61 aggaactgca ccttgggcat atccaaatcc cgcagttcct gctgcacccg atcctgaagg
      121 ccctgcgccg ccttctttct ggcttcggag agctcctgcg cccgcttttt tgcccgcgat
      181 aacctgctgc ttgtaaagct cgtatgccgt ctcaaacggt aattcgccgt acggctttaa
      241 gagctttccc gtaggtccca aatcaagagc gacaaaggtt ttatccttgc cggacacggc
      301 cgttttagct atatctatcg cggaagtaac aatttcctcc accgtgtaat cggtaccctt
      361 gaatttcagc gcatttgcac cgaaggtgtt tgattttatt atattgcagc cggcgtccgc
      421 atacgccttg tgaatggcat aaacggtatc ccgatgagta atattccatg tttcgggaat
      481 ttcgccggca gaaagtccgt ttttttggag cattgtaccc atggctccgt caaaaaacaa
      541 tctctcttta cctaaaaggt cgataaattt catagcttaa cctcttctga attcgcagtt
      601 tttatttccg catgcggcgc aggcgttaaa attacagtcc ttatccttgg taagccctat
      661 aaacgccgta acggactttg tgggtatcat catacaattc tccgacaggg taagccctat
      721 gcgcttggtg cagtcaagca gcttaaaaaa ctccttttgg ctctcaagcg acaaatcggc
      781 ataaccgggg ctgaagcgcg gtcttaaata atagcctcgc ttctcctctt cctttgctat
      841 attttcgcag acgtcatcgc aaaagctttc gacagccgcc gccgttgccg cttgcataac
      901 cgccgctcgc gcagtgtcaa ctcccgcgta ccgtcttacg agaatatcgc tctgcatacc
      961 gagcgttgcg gcaaaaagca atattctgtc ggaatttctc aaatgcttca caagcttttc
     1021 ggagtgaaat tcgataccgt caagctttac ggcactgtcg gacaggtatt cgagtttgca
     1081 ctctctgtac agacttctcg gctgaatcga cgt
//
LOCUS       DBMDVO010000611         1111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60409_length_1111_cov_0.943564, whole genome shotgun sequence.
ACCESSION   DBMDVO010000611 DBMDVO010000000
VERSION     DBMDVO010000611.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60409_length_1111_cov_0.943564"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="ACZPE2_07860"
     CDS             complement(<1..367)
                     /locus_tag="ACZPE2_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1319800.1"
                     /translation="MSKLVIVESPHKATMIKKYLGKSYDVTASVGHIRDLPAAKLSVD
                     IKNDFAPKYAIVKGKEKLVKELKEKAANADSTERAVAMLSALPCGLISMSRDIEGLPE
                     TSLNLGILKTEESEISAEFC"
     gene            complement(409..>1111)
                     /locus_tag="ACZPE2_07865"
     CDS             complement(409..>1111)
                     /locus_tag="ACZPE2_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1319801.1"
                     /translation="DYLRDNAALRREISKNGAVITEYFPFTPASRTTFPMRNRIISGM
                     SLGTIVVEAGERSGSLITARLALSEGRDVFAVPGEVITSAFTGTNRLIHDGAKPVFTA
                     LDVLEEYYYTYPDKIDLSGADKPLGEALKNMPIEEVSVPKAVHSAVRQKSKTAQKKAA
                     ENKTPQKSAPRELPDSLTENAKKLYSVLRTEPRHIDDLSEEAKLSSADTLAALTELEL
                     YSLVALSEGKHYYKV"
BASE COUNT          241 a          296 c          256 g          318 t
ORIGIN      
        1 tacaaaattc cgccgaaatt tcactttctt cggtcttcaa tatgccgagg ttgagcgagg
       61 tttccggaag accctctatg tcacggctca ttgagataag tccgcacggg agggctgaca
      121 gcatagcaac tgccctttcg gtagagtcgg catttgcggc tttttccttt aattccttga
      181 caagcttctc cttgcccttg actattgcgt atttcggtgc aaaatcgttt ttgatgtcaa
      241 ccgagagctt tgccgccggc aaatccctta tgtgcccgac ggacgcggtt acatcgtaac
      301 tttttccgag atattttttt atcattgtcg ccttgtgcgg cgactccact ataacaagct
      361 ttgacataaa caaactcctg tttctcgata ataacggctt ttgccgtatc agaccttata
      421 ataatgcttt ccctcagaga gggcgacgag cgaatacagc tcgagttctg taagagcggc
      481 aagtgtatcg gcggaggaga gcttcgcttc ctcgctcaaa tcatcaatat gccgcggctc
      541 ggttcttaag accgaataca gttttttcgc attttccgta agcgagtcgg gaagctccct
      601 cggcgcgctc ttctgcggag tcttattctc cgccgctttt ttctgtgccg tctttgattt
      661 ttgccttacg gcactatgaa cggctttcgg aacggagact tcctcaatcg gcatattttt
      721 aagagcctca ccgagcggct tatccgctcc gcttaaatca atcttatccg ggtaagtata
      781 ataatattct tccaaaacgt caagtgccgt aaacacgggc tttgctccgt cgtgtatcag
      841 gcggttggtt ccggtgaacg cagaggttat gacttcgccc gggacggcaa acacgtctct
      901 gccctccgag agagcaagcc ttgccgtaat aagcgagccg ctcctctcgc cggcctcaac
      961 cactatcgtg ccgaggctca tccccgaaat tatcctgttt ctcatcggga atgttgttct
     1021 cgacgcagga gtaaacggga aatactccgt tataaccgca ccgttttttg atatttcacg
     1081 ccttagagcg gcattgtcgc gcaaatagtc g
//
LOCUS       DBMDVO010000612         1111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60414_length_1111_cov_0.885149, whole genome shotgun sequence.
ACCESSION   DBMDVO010000612 DBMDVO010000000
VERSION     DBMDVO010000612.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60414_length_1111_cov_0.885149"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="ACZPE2_07870"
     CDS             complement(<1..597)
                     /locus_tag="ACZPE2_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1319802.1"
                     /translation="MKVYHGNILTVDKNDTVAEYLVENCGRIVYVGDSLPEAFKDAKT
                     IELREKALVPSFADTHIHFASFATFYAGLNVMEAKSNSEILEMLQSHVRKSKEKIITG
                     FGASPYSVSDGHLVRRTELDRVCPDKPVFIVKYDGHACVINTALLNKIRDKIKGLRGF
                     HEDTGEMNQEAFFAVSDYVTNSVSPLHLIKNMQKAADYM"
     gene            854..>1111
                     /locus_tag="ACZPE2_07875"
     CDS             854..>1111
                     /locus_tag="ACZPE2_07875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1319803.1"
                     /translation="MLKQKEMPAVGEKIAVIKTDKGDIKVRLFPEEAPKAVENFVTHA
                     ENGYYDGLIFHRVIKDFMIQGGDPKGTGTGGESIWGKPFADE"
BASE COUNT          316 a          225 c          246 g          324 t
ORIGIN      
        1 catatagtcg gctgcctttt gcatattctt tataaggtgc aacggtgaaa cagaatttgt
       61 aacataatcc gaaacggcaa aaaacgcttc ttgattcatt tcacctgtat cctcgtgaaa
      121 gccgcgaaga ccttttattt tatcacggat tttatttaaa agagcagtgt ttataacgca
      181 ggcgtgaccg tcgtatttaa ctataaacac aggcttgtcg gggcagacgc ggtcaagctc
      241 ggtgcgcctg acaagatgac cgtcggacac cgaatacgga gaggctccga agcctgtaat
      301 tattttttcc ttactttttc ggacgtgaga ctgcagcatt tcaagtattt ccgagttgct
      361 ttttgcctcc ataacgttaa gaccggcata aaaagtggca aagctcgcaa aatgtatatg
      421 agtatcggca aatgacggta ccaacgcctt ttcacgaagc tctatcgtct ttgcgtcttt
      481 aaaagcctcc ggcagactgt caccgacgta aactatcctg ccgcaatttt caacaagata
      541 ttcggcgaca gtatcgtttt tatctaccgt aagtatgttt ccgtgataaa ctttcatatt
      601 tttcactcct aaataaaaaa gaagcgagct gtacaactcg cttctttgct ttaaattatt
      661 atattatcgc aaaccgtatt tgtcaacaat tattctgttt tgcacacaat accgctttgg
      721 tgtttatcac ttttcgggga attgttcatt gccgatttcg tgtttgtgtg atattattaa
      781 attgatgatg tatccgattt gtcgcagaga aaaccgggtg caatttataa gtgtattatg
      841 ggagttggaa caaatgttaa agcaaaaaga aatgccggct gtcggcgaaa aaatagcagt
      901 tatcaagacc gacaagggcg atattaaggt gagacttttc ccggaggaag cacccaaagc
      961 ggttgaaaac tttgttacgc acgctgaaaa cgggtactat gacggactta tattccacag
     1021 agtaattaag gattttatga ttcagggcgg tgaccccaag ggtaccggaa cgggcggcga
     1081 gagcatttgg ggcaagccgt ttgccgatga a
//
LOCUS       DBMDVO010000613         1107 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60657_length_1107_cov_1.265408, whole genome shotgun sequence.
ACCESSION   DBMDVO010000613 DBMDVO010000000
VERSION     DBMDVO010000613.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60657_length_1107_cov_1.265408"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="ACZPE2_07880"
     CDS             complement(<1..514)
                     /locus_tag="ACZPE2_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1319804.1"
                     /translation="MAEKKTRLDVAVFERGLTDSRAKAGALIMAGQVFVNGQKALKSG
                     QNVKEDDQIEVRGEKMPFVSRGGYKLDKAVKAFDLSLQDKICMDIGASTGGFTDCMLQ
                     NGAAKVFSVDVGYGQLAWKLRTDERVVNMERTNFRYLTIDDIGTKLDFASVDVSFISL
                     KIILPVLFELL"
     gene            complement(489..>1107)
                     /locus_tag="ACZPE2_07885"
     CDS             complement(489..>1107)
                     /locus_tag="ACZPE2_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1319805.1"
                     /translation="EDGISHQGILDAAFLNGIPGITVYSPSTYTELKHAFIRAIYHSN
                     GVVAIRYPRGTEKPVPESNDLSFEPFAVYGREDSDTFIVTYGRLYSYACSAADELISR
                     GYNVSVLKLNRIKPISEDAVKKVLSCKKVFFFEEGIKAGGIGSVFSEMMTEKGFKGNF
                     NLTAVDNEFVKHAAVSALLSEYSLDENGMIKTILEYDDGGKKDEA"
BASE COUNT          287 a          286 c          223 g          311 t
ORIGIN      
        1 ttaagagctc aaaaaggacg ggcaaaatga ttttaagcga tataaatgag acatccacac
       61 tcgcaaaatc aagctttgtg ccgatatcgt caattgtaag gtatcggaag tttgtgcgtt
      121 ccatattcac aacgcgttca tcggtgcgaa gcttccatgc gagctgaccg tatcccacat
      181 caaccgaaaa cacctttgcc gctccgtttt gcagcataca atctgtaaat ccgcccgttg
      241 acgcgcctat atccatacat attttatctt gaagtgaaag atcaaatgcc ttaaccgcct
      301 tgtcgagctt atagcctccg cggctgacaa acggcatttt ttcgccgcga acctcgattt
      361 gatcgtcttc tttcacattt tgacccgact taagagcctt ttgcccgttt acaaagacct
      421 gacccgccat aattagcgcg cctgccttcg cacggctgtc cgtaagtccg cgttcaaaaa
      481 cggctacgtc aagcctcgtc tttttttccg ccatcgtcat actccaaaat tgtttttatc
      541 ataccgtttt cgtcaagcga atactcgctt aaaagcgcgg aaaccgctgc gtgctttaca
      601 aattcattgt ctaccgccgt taaattgaaa ttgcctttaa agcctttttc cgtcatcatt
      661 tctgagaata ccgagcctat accgccggct ttgatgccct cttcaaagaa aaacactttt
      721 ttacatgaga gaaccttttt aacagcgtcc tccgagatag gctttatgcg attaagcttt
      781 aaaacagaga cgttatatcc gcggcttata agctcgtctg ccgcggagca ggcataggaa
      841 taaagtctgc cgtatgtgac aatgaacgta tccgagtctt ctctgccgta aaccgcaaac
      901 ggttcaaacg aaagatcgtt cgactccggt acgggctttt cggtaccgcg cggatagcgt
      961 atggctacca caccgttact gtggtatatc gcccttataa aagcgtgttt taattccgtg
     1021 tatgtagacg gtgaataaac cgttataccg ggtattccgt ttaaaaatgc tgcatccaaa
     1081 ataccttggt gcgaaatgcc gtcctcg
//
LOCUS       DBMDVO010000614         1104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60923_length_1104_cov_1.074776, whole genome shotgun sequence.
ACCESSION   DBMDVO010000614 DBMDVO010000000
VERSION     DBMDVO010000614.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60923_length_1104_cov_1.074776"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="ACZPE2_07890"
     CDS             complement(<1..273)
                     /locus_tag="ACZPE2_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1319806.1"
                     /translation="MLDLIISTLTQGFIYALLSFGVYITYKILDFPDLTVDGSFPLGA
                     AVTAVLLVKGVNPFLALLISFLVGALAGFVTGFIHVRLKVRDLLSGI"
     gene            complement(288..>1104)
                     /locus_tag="ACZPE2_07895"
     CDS             complement(288..>1104)
                     /locus_tag="ACZPE2_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1319807.1"
                     /translation="LLGLKEAGLTEGEDYTIDYQNASFDNATATQIANNFSSKNVALM
                     CAIATPSATACYAAAEDKNIPVVFNAITDPGEAGLTTGNITGVSDKLPVDPQLELIRK
                     LQPDAKTIGIIYTTSEPNSVSAIAEYKEKAGNYGFTIEAIGVADQASVTQAADTLINK
                     KVDCITNLTDNNVVGVLPSILEKTNAAGIPVYGSEIEQVKKGCVASAGIDYVELGKMA
                     GKLAAQILKGEAKASDIPYETVTEYSTYINSDAASAMGITVPSDVAAKAIECK"
BASE COUNT          283 a          250 c          258 g          313 t
ORIGIN      
        1 aatacccgaa agcaggtcgc gtaccttcaa acggacgtgg ataaatcccg tgacaaatcc
       61 ggcaagagct ccgacaagaa aagatattaa aagagcgaga aacggattta cgccctttac
      121 aagtaaaaca gccgttacgg cagctcccaa agggaagctg ccgtctacgg tgagatcggg
      181 aaaatccaat attttatatg taatatatac gccgaaggaa agaagagcgt atataaagcc
      241 ttgagttaac gtgcttataa taagatccag cattctttta cactacctta tttacattct
      301 attgcctttg cggctacatc cgacggaacg gtaattccca tagcgcttgc tgcgtcggaa
      361 tttatatatg tgctgtactc ggtcactgtt tcgtacggaa tgtcggaagc cttagcttct
      421 ccctttaaaa tttgagcggc aagcttacct gccattttac cgagctccac ataatcgata
      481 ccggcagacg ccacacagcc ttttttaacc tgctcaattt cgctaccgta tacgggaata
      541 cctgcggcat tggttttttc aagtattgac ggaagaacgc ctacaacatt attgtcggta
      601 aggtttgtga tgcaatcaac cttcttattt ataagagtat cggcagcctg tgtaacggac
      661 gcctgatcgg caacgcctat ggcttcgata gtaaatccgt agttgcctgc tttttcttta
      721 tactcggcga ttgctgatac cgaattcggt tcgcttgttg tgtagatgat accgattgtt
      781 tttgcatcag gctggagttt tcttataagc tcaagctgcg ggtcaacggg aagcttatcc
      841 gaaacgcccg tgatgttgcc tgttgtaaga cctgcttcac cggggtctgt tatggcgttg
      901 aatacaaccg gaatgttctt atcctctgcc gcagcgtaac aagctgttgc cgagggggtt
      961 gctatggcgc acataagtgc gacgtttttg gaagagaagt tattggcaat ctgtgttgcg
     1021 gtggcattgt caaacgaagc attctgataa tctattgtgt aatcctcacc ctctgtgagt
     1081 ccggcctcct taagtccgag aagg
//
LOCUS       DBMDVO010000615         1103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_60970_length_1103_cov_1.393214, whole genome shotgun sequence.
ACCESSION   DBMDVO010000615 DBMDVO010000000
VERSION     DBMDVO010000615.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60970_length_1103_cov_1.393214"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            298..570
                     /locus_tag="ACZPE2_07900"
     CDS             298..570
                     /locus_tag="ACZPE2_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319808.1"
                     /translation="MDFKTVPIKLSDEIKEVMENRGIREEDIREVLEYAETTGKKLYI
                     EGEEHYLAKKRLNKFTPNVEYIIADGEVEVVNLYSYIISFADPKGE"
     gene            583..771
                     /locus_tag="ACZPE2_07905"
     CDS             583..771
                     /locus_tag="ACZPE2_07905"
                     /inference="COORDINATES: protein motif:HMM:NF048774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7479 domain-containing protein"
                     /protein_id="HIR1319809.1"
                     /translation="MNWKCFNCKVDMEEVDDIAITYNDMDLPDAEGYRCPNCGLAFLD
                     AQFVVGELASAEEMLTGK"
     gene            793..>1103
                     /locus_tag="ACZPE2_07910"
     CDS             793..>1103
                     /locus_tag="ACZPE2_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013986.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR1319810.1"
                     /translation="MKLFAGVDIGSTTSKCIIIDESGSVLTFQVLYTEFDRNLSGEKV
                     LKAAMDAIGVTEQDIAYIISTGYGRKAFLKANDNIPEIIAHGIGTHVVAPAARTIIDI
                     GG"
BASE COUNT          318 a          211 c          296 g          278 t
ORIGIN      
        1 gcttgatgtg atggtgttct caatatcgta ccagtgcgct gcgggtataa taagcatgac
       61 ggcggaggag tacgaagagt tgatagattt cttccttaaa aagtgcctta cgtgaaagcg
      121 agtcaaaaat aaataagttt gataaaacat atactattga cattttcgtg gcaatagtat
      181 atatttatat aggaaattta cactgtaagt tttcaaagag cacagtattt ttccgacgct
      241 tcccgctttc gcgggggcgg gtcgggatga aaaaataaaa atggaggtat gactgcgatg
      301 gattttaaaa ccgttcccat caagctgagc gacgagatca aggaggtcat ggaaaaccga
      361 gggatccgtg aagaggacat cagagaggtc cttgaatacg ccgagaccac tgggaagaag
      421 ctctatatcg agggcgagga gcactatctt gcaaagaaga ggctgaacaa gttcactccc
      481 aacgtggagt atattattgc tgacggtgag gttgaggtcg ttaatctcta tagctatatt
      541 ataagtttcg ctgatccaaa gggagaataa ggagggctta aaatgaattg gaagtgcttt
      601 aactgcaagg ttgatatgga agaagtggac gatatcgcga taacctataa cgatatggac
      661 ctgccagatg ccgagggcta cagatgccct aattgcggcc tcgcttttct ggacgcccag
      721 ttcgtagtcg gtgagcttgc ctctgccgaa gagatgctta ccggtaagta aatatataaa
      781 gagggttttg ctatgaagct ttttgccggt gtggatatcg gctctaccac gtccaaatgc
      841 atcatcattg atgagagtgg cagtgtcctc acgttccagg tattgtacac tgagtttgac
      901 agaaatctga gcggcgaaaa ggtgctaaaa gccgcgatgg acgccattgg tgtgacggag
      961 caggatattg cttatatcat atccaccggg tacggccgca aggcgtttct caaggctaac
     1021 gacaatattc cagagattat tgcccacggc atcggtacac acgtagttgc tccggcggcg
     1081 cgcacaataa tagatatcgg agg
//
LOCUS       DBMDVO010000616         1102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61041_length_1102_cov_1.410589, whole genome shotgun sequence.
ACCESSION   DBMDVO010000616 DBMDVO010000000
VERSION     DBMDVO010000616.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61041_length_1102_cov_1.410589"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="ACZPE2_07915"
     CDS             complement(<1..1073)
                     /locus_tag="ACZPE2_07915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1319811.1"
                     /translation="MIFVVMSLIAVAVWAVLLEWLCSVSKYYKAKKNWLRSTLVWYVI
                     RFIGKLFSPLKRLREKPVRLRRRVLLALIGYIIGNTVLTGVAAMCRGFWFVLLIAVLL
                     LYNILTVSLMWKYVLSLDRIIAASENAKNGEPPENIYSENMPEPLKTLALNLSLTQEE
                     MQKAINEAVKGERMKTELITNVSHDLKTPLTSIISYVDLLKKCDIDDISAQKYINVLD
                     EKSIRLKRLIEDLVEASKASSGAVTFNKMNVNLHELAVQAVGEMSDAFEERNLDAVLN
                     EPTEPIVIYADSQKTWRIIDNLLSNVKKYALPGTRVYIDVGRDSSYGTITIKNTSREK
                     LNIDPDELTQRFVRGDESRTMEG"
BASE COUNT          270 a          285 c          237 g          310 t
ORIGIN      
        1 ctgccctcca tggtacgtga ctcgtctccg cgtacaaagc gctgagtcag ctcatcgggg
       61 tctatattca gcttttcgcg tgaagtgttt tttatcgtta ttgtaccgta tgagctgtcc
      121 ctgccgacgt ctatatatac tctcgtaccg gggagagcat actttttgac gttgctcaaa
      181 agattgtcta tgattcgcca cgtcttttgg ctgtcggcgt atataacgat cggctccgtc
      241 ggctcgttga gcaccgcatc gagatttctc tcctcaaaag cgtcggacat ttcaccgacc
      301 gcctgaaccg caagctcatg gagattaaca ttcatcttgt taaacgtcac ggctcccgat
      361 gacgcctttg acgcctcgac aaggtcctcg ataagtcttt tgagtcttat ggatttttca
      421 tcgagcacgt ttatatattt ttgtgccgaa atatcgtcta tgtcgcactt tttgagcaaa
      481 tcgacatatg agattatcga agtgagcggc gttttaaggt cgtgcgacac atttgttata
      541 agctcggttt tcattctttc gccctttacc gcctcgttta tcgccttttg catctcctcc
      601 tgcgtaaggc tgaggttgag cgccagcgtt tttaagggct caggcatatt ttccgaatag
      661 atgttttcgg gaggctctcc gttttttgcg ttctccgacg ccgctattat gcggtcaagc
      721 gacagcacat atttccacat aagcgaaaca gtcagaatat tatacagcag cagcaccgct
      781 ataagaagaa cgaaccaaaa tcctctgcac atagccgcaa cgccggtcag cacggtgttg
      841 ccgattatat agcctataag cgcaagcagt acccggcgcc ttaagcgcac cggcttttca
      901 cgaagccttt taagcggcga aaataatttc ccgataaacc ttataacata ccaaacaagc
      961 gttgatcgaa gccagttctt tttcgccttg taatatttag aaacagaaca gagccactca
     1021 agcagtacag cccacaccgc aacggcaata agcgacataa ccacaaatat cataagcgct
     1081 ttgccgtcgg catagtaatc ga
//
LOCUS       DBMDVO010000617         1101 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61122_length_1101_cov_1.263000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000617 DBMDVO010000000
VERSION     DBMDVO010000617.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61122_length_1101_cov_1.263000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            6..368
                     /locus_tag="ACZPE2_07920"
     CDS             6..368
                     /locus_tag="ACZPE2_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1319812.1"
                     /translation="MDKKILLVDDAAFMRMLIRNTLTTNGYTNILEAADGEIAVSTYS
                     AEKPDLVIMDITMPNKTGIEALKEIKAMDPGAKVVMCSAMGQEAMVVEAIKLGALDFI
                     VKPFKAERILQTVKKILD"
     gene            368..841
                     /gene="fliO"
                     /locus_tag="ACZPE2_07925"
     CDS             368..841
                     /gene="fliO"
                     /locus_tag="ACZPE2_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HIR1319813.1"
                     /translation="MEYVQLVCALIGVLALIFLFFWILRKVNKGILSVSGKRLKVLDR
                     ASLGGEKSVVVVSVAGKCMVLGVTAGRIDKIEDLSITEEEYMSELYPEGSGKQDNFFA
                     AFSDAMAKNIKNMRGKKASRDEEPDCGDELPDVRKTDDGTNSDDKNSDERNTESK"
     gene            838..>1101
                     /locus_tag="ACZPE2_07930"
     CDS             838..>1101
                     /locus_tag="ACZPE2_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HIR1319814.1"
                     /translation="MTRAFGLTIRKMFIDNKRIFIRFIVSLTVCIALIGVLCSVTAFA
                     ADNNASGNAGTTTSIGDTASANTSGGTQSADSSVMKDLIGVDGS"
BASE COUNT          316 a          226 c          302 g          257 t
ORIGIN      
        1 cacttatgga taagaagata ttacttgtag acgatgcggc attcatgcgt atgcttatcc
       61 gcaacacact tacgacaaac ggctacacaa atatacttga ggcagctgac ggcgaaatag
      121 cggtttcgac atattccgca gagaagcccg accttgttat aatggacata acaatgccca
      181 acaagaccgg tatcgaggct ctcaaggaaa tcaaggctat ggatcccggc gctaaagtcg
      241 taatgtgcag tgctatgggt caggaagcta tggtagtaga agctatcaag ctcggcgcac
      301 ttgacttcat cgtaaagccg ttcaaggctg aaagaatact tcagaccgtt aagaaaatac
      361 ttgattgatg gaatacgtac agcttgtatg tgcgcttatc ggcgtacttg cactgatttt
      421 tctgttcttc tggatactga gaaaggtaaa caaggggata ctgagcgtaa gcggaaaacg
      481 gctcaaggtg cttgaccgtg cctcgctcgg cggtgaaaaa tcggtggttg tcgtgagcgt
      541 tgcaggaaag tgtatggtgc ttggcgtaac tgccggcagg atagacaaga tcgaggatct
      601 gtcgattacg gaagaagaat atatgtctga gctttatcct gagggaagcg gaaagcagga
      661 taacttcttc gccgccttca gtgacgcaat ggctaaaaac ataaagaata tgagagggaa
      721 gaaagccagc cgtgacgaag aaccggattg cggcgatgag cttcccgatg ttcgcaaaac
      781 cgatgacgga acaaattccg acgacaaaaa ttctgacgag aggaacacgg agtcgaaatg
      841 acaagagctt ttggtcttac gattagaaaa atgtttatcg acaacaagcg gatttttatc
      901 cgctttatcg tgtctttaac ggtatgtatc gcacttatcg gtgtgctgtg cagtgtaacg
      961 gcttttgcgg cggataacaa tgcgtcaggc aacgcaggta cgacaacaag tatcggcgat
     1021 acggcttcgg caaacacctc gggcggtacg cagtcggcgg attcgtcggt gatgaaggat
     1081 cttatcgggg tagacggctc g
//
LOCUS       DBMDVO010000618         1099 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61298_length_1099_cov_0.844689, whole genome shotgun sequence.
ACCESSION   DBMDVO010000618 DBMDVO010000000
VERSION     DBMDVO010000618.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61298_length_1099_cov_0.844689"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="ACZPE2_07935"
     CDS             complement(<1..683)
                     /locus_tag="ACZPE2_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319815.1"
                     /translation="MAKRCPFCQKEMPDEANFCLNCFSALGTEASPESCGAKNGNHDE
                     KPTHKHTRKHISKPKAENALPLVNKKARRKIFTAAILACTFLLCGILAGLTRKQIRTA
                     ALGEPETMAALVTDENGEAVTDENGDNVYEYVEVPQEEKGFFGKLFGSFMHNKDDAEG
                     TDNTYAENGSQGEAVPGKAEGSVYSTGNTGSGTVGSNTGSSKTEENITDNSPPSSEGQ
                     KAPWPIWDWT"
     gene            complement(708..>1099)
                     /locus_tag="ACZPE2_07940"
     CDS             complement(708..>1099)
                     /locus_tag="ACZPE2_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1319816.1"
                     /translation="NDGKVPIENYIQSNSDSFINIDLSKFWKELINKSGMIKTDIINK
                     SDFSYVYFYDVINGRKIPSRDKIIRLALALGLSLDECQTALKFCGRSQLYPRIKRDSI
                     IIHGINRKLSIYEVSDKLLSFGEEDLK"
BASE COUNT          234 a          263 c          242 g          360 t
ORIGIN      
        1 gtccagtccc atattggcca tggtgccttc tgcccctccg atgacggcgg agaattatct
       61 gtaatgttct cttcggtttt gctgctcccc gtattgctgc cgacggttcc cgaacccgta
      121 tttccggtcg aataaacgga gccctcggct ttgcccggca ccgcttcgcc ttggctgccg
      181 ttttcggcgt atgtattgtc ggtgccttcg gcgtcgtctt tgttatgcat aaaactgccg
      241 aaaagctttc cgaaaaagcc tttttcctct tgcggtactt caacatattc gtaaacattg
      301 tcgccgtttt catcggtgac ggcttccccg ttttcgtcgg taacgagagc cgccatggtt
      361 tcgggctcac ccaaagctgc tgtgcgtatt tgttttcttg tgagtccggc aagaataccg
      421 cacagcagaa aggtgcatgc aagaatcgcc gccgtaaata tttttcgccg cgcttttttg
      481 ttgacgagcg gtaaagcatt ttccgcttta ggcttgctta tatgctttct agtatgcttg
      541 tgtgtcggct tttcatcgtg attgccgttt tttgcgccgc agctttccgg tgatgcctct
      601 gtgccgagtg ccgagaaaca gttcaggcag aaattagcct catcaggcat ttctttctga
      661 caaaacggac atctttttgc cattttttcc ccgtccttta aaaaatgtta ttttaaatct
      721 tcttctccaa atgatagaag cttgtccgaa acctcgtata tgctgagttt tcggttgatg
      781 ccgtggatta taatactgtc gcgctttatc cgtgggtaaa gctgtgatct tccgcaaaat
      841 ttaagggcag tttgacactc gtcaagcgaa agaccgagtg ccaaggcaag acgaattatc
      901 ttatcgcgcg acggaatttt tctgccgtta atcacatcgt aaaaatacac gtagctgaag
      961 tccgatttgt tgataatatc ggtttttatc attcccgatt tatttatcag ttctttccaa
     1021 aatttactca aatcgatatt gatgaaagag tctgaattgc tttgaatata gttttctatc
     1081 ggaaccttac cgtcgttta
//
LOCUS       DBMDVO010000619         1098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61335_length_1098_cov_1.225677, whole genome shotgun sequence.
ACCESSION   DBMDVO010000619 DBMDVO010000000
VERSION     DBMDVO010000619.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61335_length_1098_cov_1.225677"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="ACZPE2_07945"
     CDS             complement(<1..334)
                     /locus_tag="ACZPE2_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1319817.1"
                     /translation="MHIKSYCAAHRHGLFSPQSNTGRAVLSLAFALVLTVVGLISIFD
                     TDKTVSEEENRKLAAKPKFSFTALFDKSYTSDFDTYYADTFPFRDEFLGLNRKISEIL
                     TGTKGSGDI"
     gene            complement(352..>1098)
                     /locus_tag="ACZPE2_07950"
     CDS             complement(352..>1098)
                     /locus_tag="ACZPE2_07950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1319818.1"
                     /translation="SSAGAWIGIISYTLQIYFDFSGYSDMAIGLGHMFGFSFPENFNY
                     PYYAVSVSDFWRRWHITLSSFFRDYLYIPLGGNRKKWALNMLIVWLLTGLWHGASWNF
                     VLWGLYYFVFLMLEKMYIGKFLEKLPRAVGHIYSLIVIIIGWVFFYFDDLSKIKYFFK
                     AAFGAANGFVTLTEKTVIVNYAVLLIIGAVCAMPLLPRIKNFACSLSKTRSGKVTVDV
                     LSVLFVIFSLAISTASLVGNSYNPFLYFRF"
BASE COUNT          345 a          254 c          232 g          267 t
ORIGIN      
        1 caatgtctcc cgagcctttg gtccccgtga gtatttccga tattttcctg ttgagtccga
       61 gaaattcatc tctgaaagga aatgtatcgg cgtaatatgt atcaaaatcc gaagtatagc
      121 tcttatcaaa aagtgccgtg aacgaaaact tgggctttgc cgcaagcttt ctgttttcct
      181 cttcggatac ggttttatcg gtatcgaata tcgatataag tcctacaacg gtcaaaacaa
      241 gcgcaaaagc gaggcttaaa accgccctgc ccgtgtttga ctgcggactg aataagccgt
      301 gtctgtgcgc ggcacagtag ctttttatat gcaaaaaagg tcttcccttc gttaaaatct
      361 gaaataaagg aacggattat agctgttccc gacaagcgac gccgttgaaa ttgcaagcga
      421 aaaaatcaca aacaaaaccg ataatacgtc cacggtaact tttccgcttc gcgtctttga
      481 aagagagcag gcaaaatttt tgatgcgcgg caaaagcggc atagcacaca cggcacctat
      541 tataagcagc accgcataat ttacaattac ggtcttttcc gtaagcgtaa caaatccgtt
      601 tgcggcgccg aacgccgctt tgaaaaagta tttaatcttg gataagtcgt cgaaatagaa
      661 gaacacccat ccgataatta taacaatcag cgaataaata tgcccgactg ctcgcggcaa
      721 tttctcgaga aactttccga tatacatttt ttcaagcatc aggaacacaa aataataaag
      781 tccccaaagg acaaagttcc agcttgcacc gtgccaaaga ccggtgagaa gccaaactat
      841 aagcatattc agtgcccatt ttttacgatt accgccgagc ggtatgtaaa ggtaatctct
      901 gaaaaacgac gacagcgtta tatgccagcg gcgccaaaaa tcagaaacag acacggcgta
      961 atacgggtaa ttgaaattct cggggaacga aaagccgaac atatgcccga ggcctattgc
     1021 catatcggaa tagcccgaaa aatcgaagta gatttgcagg gtgtatgaga ttatacctat
     1081 ccacgctccc gcgcttga
//
LOCUS       DBMDVO010000620         1097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61418_length_1097_cov_1.171687, whole genome shotgun sequence.
ACCESSION   DBMDVO010000620 DBMDVO010000000
VERSION     DBMDVO010000620.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61418_length_1097_cov_1.171687"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="ACZPE2_07955"
     CDS             <1..252
                     /locus_tag="ACZPE2_07955"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HIR1319819.1"
                     /translation="IKRCEGKVDAQETAIGYIPYAKDINIEGLEGEVSLESLEKILDV
                     DKNLWEEEANGIEEFFKKFGDKLPKELKESLETLKANLK"
     gene            401..>1097
                     /locus_tag="ACZPE2_07960"
     CDS             401..>1097
                     /locus_tag="ACZPE2_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1319820.1"
                     /translation="MASPIGKAKSLKFKKDGTFRIMQIADTQDTNITSKNTVEFIGKA
                     LDSENPDLVVFTGDQIKGYGFSFATGDRDKKVAEAIRNIVTPVAERNIPFTFVFGNHD
                     DQAFGISKEKQMIVYKSFPTCLAEPGDPSISGYGNHYINILSSDGKKILFNIYLIDSL
                     SASLDGHCSAVSEEQIAWYKSVRDYLKDKTGDYVPSLLFQHIPVPEMWNVLKEVPKSH
                     KPHAVGYRSHYGKY"
BASE COUNT          343 a          202 c          244 g          308 t
ORIGIN      
        1 attaaacgct gtgaaggtaa agttgatgct caggaaacag ctatcggcta tattccttat
       61 gcaaaagaca ttaacattga aggccttgaa ggtgaagttt cactcgaatc gcttgaaaag
      121 attcttgatg ttgataagaa tctttgggaa gaggaagcaa acggtattga agagttcttc
      181 aagaagtttg gcgataagct tcccaaggag cttaaagaga gtctcgaaac tcttaaggca
      241 aaccttaaat aagttaaaat ttttcaatca ccgcacagtt attgctgtgc ggtgattttt
      301 tatgttcata tagataaagt gtgttctgct gtgagttttg acttttattt tgcgcgatta
      361 tgtgttatac ttttgacata aaaaagaaag ttggtgcgtt atggcttctc cgataggcaa
      421 agcgaaaagc ttaaaattta agaaagacgg cactttccgc attatgcaga tagccgacac
      481 tcaagataca aacatcacgt caaaaaacac ggtggaattt atcggtaaag cgcttgatag
      541 cgaaaacccc gaccttgtag tgtttacggg tgaccagata aaaggctacg gcttcagttt
      601 tgccacgggt gatcgcgaca aaaaggttgc cgaagcaata cgtaatatcg taacaccggt
      661 tgccgaaaga aacattccgt ttacatttgt attcggtaat catgatgatc aggctttcgg
      721 tatttccaag gaaaagcaga tgatagtata caagagcttt cctacctgtc ttgcagaacc
      781 cggcgatcct tctatcagcg gatacggtaa ccattacata aatattctaa gctcggacgg
      841 caaaaaaata ttatttaata tatatcttat agactctctt tctgcgagtc ttgacggcca
      901 ttgcagtgcg gtttccgagg agcagattgc ttggtacaag agtgtcagag attatttaaa
      961 agacaaaaca ggggattacg tgccgtcttt attgttccag catataccgg tgcccgaaat
     1021 gtggaacgtg ctgaaagaag ttccgaaatc acataagccg cacgctgtcg gatacaggtc
     1081 tcattacgga aaatact
//
LOCUS       DBMDVO010000621         1095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61553_length_1095_cov_1.792757, whole genome shotgun sequence.
ACCESSION   DBMDVO010000621 DBMDVO010000000
VERSION     DBMDVO010000621.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61553_length_1095_cov_1.792757"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="ACZPE2_07965"
     CDS             complement(<1..967)
                     /locus_tag="ACZPE2_07965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1319821.1"
                     /translation="MKRAVSIMTATVIFISIFSVGAFAANTPAEDFPPMQVEINAKAA
                     LLMEAETGKVLMASDPHEKLPPASVTKIMSLLLVAEAIDDGKISLTDEVTVSTEASKM
                     GGSQIWLKENEVMTVDDLLKATAVYSANDACTALSEYVGGSTSAFVRMMNERAKELGM
                     NDTNFENCTGLDDTVTQHYTSCYDIALMSRELLKHKMITNYTTIWMDTLRGGKTQLVN
                     TNKLIRFYNGATGLKTGTTDKAGCCVSATAERDGMSLIAVVLGSDNSKDRFSAARAML
                     DWGFANYSKVTVSVDSDKITSVRVLGGTEDYITPFLSKEMTVVLKK"
BASE COUNT          276 a          281 c          204 g          334 t
ORIGIN      
        1 cttttttcag caccacggtc atttcctttg acaaaaacgg tgtaatataa tcctcggttc
       61 cccccagtac tctgacactc gtaattttgt cgctatctac ggatacagtc actttggagt
      121 aattggcaaa gccccaatca agcattgctc ttgcggcgga aaagcgatct ttgctgttat
      181 cacttccgag gactaccgct atcagcgaca ttccgtcgcg ttccgccgtc gccgacacac
      241 agcatccggc tttatctgtt gttccggttt taagacccgt tgcaccgtta tagaagcgaa
      301 tgagcttatt agtgttgaca agctgcgttt taccgccgcg aagagtgtcc atccatattg
      361 tcgtataatt cgttatcatt ttgtgcttta acaattcccg tgacatcaaa gctatatcat
      421 aacagcttgt gtaatgctgt gttaccgtgt catccaagcc ggtgcaattt tcaaaatttg
      481 tgtcgttcat tccgagctct tttgctcttt cattcatcat tcgcacaaaa gctgatgtac
      541 ttccgccgac atattccgaa agtgccgtgc aggcatcgtt ggcactgtat accgccgtcg
      601 cctttaacaa atcgtcaacg gtcataacct cgttctcttt aagccaaatt tgcgagccgc
      661 ccatttttga tgcctctgtg ctcacggtga cctcatccgt aagtgatatt tttccatcat
      721 caatcgcctc tgccactaac agcagagaca taattttagt aacggacgcc ggaggcagtt
      781 tttcgtgcgg atcagatgcc attaaaacct taccggtctc agcctccatt agtaaagcgg
      841 ccttggcatt tatttcaacc tgcataggcg gaaaatcttc ggcaggcgta tttgccgcaa
      901 acgcgcctac cgaaaaaatc gaaataaaaa ttacggttgc cgtcattata gatactgccc
      961 ttttcatagt ttacaccata cccctttcaa gcggttactt gcccatagta aaaaattcct
     1021 tgcttatatt tcgccgcacg atgtttttca aaaaatttaa tttattttcc cgaaaaacat
     1081 tcaataataa atatg
//
LOCUS       DBMDVO010000622         1095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61589_length_1095_cov_0.991952, whole genome shotgun sequence.
ACCESSION   DBMDVO010000622 DBMDVO010000000
VERSION     DBMDVO010000622.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61589_length_1095_cov_0.991952"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(268..>1095)
                     /locus_tag="ACZPE2_07970"
     CDS             complement(268..>1095)
                     /locus_tag="ACZPE2_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1319822.1"
                     /translation="IAKTIEKKIDSELKKMLETDREAYEKFFKAFGMQIKFGVYSDYG
                     IHKDSLKDLLLFYSSSEKKPVTLKEYVSRMKDGQKDIYYAAGETTDKIDMLPQVDSVK
                     DKGYEILYLTDYVDEFALRALSEYDGKTFVNVCTEDVDLGTEEEKEALKKENEDNADM
                     LKEMKEALGENVHEVRFTDKLKDHPVCLSSEGAISLEMQKVLNAMPDGKDAKATVVLE
                     INANHPIAEKLKSLYKDNKDSLCDYAKILYAQARLIGGMSVDDPVELSNLVCNLMTK"
BASE COUNT          281 a          250 c          209 g          355 t
ORIGIN      
        1 caaataagga cctgcgttcc acgagcattt ttcagccaaa caaatagttt cattccaata
       61 tttatgtact tgggtcatgc aataaatttc agtcatatat taccctaata aaaatttcta
      121 tttatcatat tataagtgta gcacataatc ataatagaaa caatactgct tcggcaagtt
      181 atcatgtatt ctggcccgct gacagaagtg taaagggaca cttccttatg gagtgtccct
      241 ttctgcggcg actttaatat tccgacatca ctttgtcata agattgcaaa caagatttga
      301 aagctccacc gggtcgtcaa ccgacatacc gcctataaga cgcgcctgag cataaagaat
      361 ctttgcgtaa tcacaaagac tgtctttatt gtccttataa agggacttga gcttttcggc
      421 tattgggtga tttgcattta tttcaagcac taccgtagcc ttggcgtcct taccgtcggg
      481 catcgcattc agcacctttt gcatttcgag agaaatagca ccctcgctcg ataggcagac
      541 gggatggtcc ttaagcttat cagtaaatct aacctcatga acattttctc cgagagcctc
      601 tttcatttct ttgagcatat cggcgttatc ctcgttttcc tttttgaggg cttctttctc
      661 ctcttcggtt ccgaggtcaa catcttccgt gcaaacgtta acaaaggtct ttccgtcata
      721 ttcgctgagt gcgcgaaggg caaattcatc gacataatcg gtgagataga gtatttcata
      781 gcccttgtcc ttaaccgaat ccacctgcgg aagcatatct attttgtcgg ttgtttcgcc
      841 tgctgcgtaa taaatgtcct tttggccgtc cttcatacgg gatacatatt cctttaaggt
      901 tacgggcttc ttttcgctag acgaatagaa caagagcaaa tccttgaggc tgtccttgtg
      961 aataccgtaa tcgctgtata cgccgaattt aatctgcata ccgaacgctt taaagaattt
     1021 ttcgtaagcc tcacggtcgg tttcgagcat ttttttaagc tcggaatcta tcttcttttc
     1081 tatggttttt gcaat
//
LOCUS       DBMDVO010000623         1095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61608_length_1095_cov_0.646881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000623 DBMDVO010000000
VERSION     DBMDVO010000623.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61608_length_1095_cov_0.646881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            9..524
                     /locus_tag="ACZPE2_07975"
     CDS             9..524
                     /locus_tag="ACZPE2_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HIR1319823.1"
                     /translation="MNTKNIKAAAQTLNAGDTVYLSGIVYTSRDAAHKRIFELINKGA
                     ALPYDINGAVQYFAGPTPTPKGLAIGSCGPTTSSRMDVFSPKLLDMGLGAMIGKGPRS
                     DEVCKAIVRNGSVYFCAVGGAGALASKCIKSCEVIAFSELGCESVKKLEFCEFPLTVA
                     IDCHGGNLFKQ"
     gene            544..>1095
                     /locus_tag="ACZPE2_07980"
     CDS             544..>1095
                     /locus_tag="ACZPE2_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1319824.1"
                     /translation="MDYNNFLNIRKNIIEKEFSRMNDMQKKAVFRIKGPLLILAGAGS
                     GKTTVLVNRISNLLKYGDAYNTEKSSRTPDEHDVELMQRYLAGDSSVYHEIRDVLSYD
                     APMPWQILAITFTNKAANELKERLVKMLGDNAADIWACTFHSACLRILRRNGDKLGFS
                     SHFTIYDTDDSKRVMKECQKLLGV"
BASE COUNT          328 a          219 c          252 g          296 t
ORIGIN      
        1 tttaccacat taatacaaag aatatcaagg cggctgcgca aacccttaac gccggcgata
       61 cggtatattt gagcggaatc gtgtatacgt cgcgtgacgc tgcccacaaa aggatatttg
      121 aacttataaa taaaggagca gctcttcctt atgatataaa tggggctgta cagtattttg
      181 caggtcctac tccgactccg aagggattgg caataggcag ctgcgggcct actacttcaa
      241 gcagaatgga tgttttttct ccaaaacttt tagatatggg ccttggagcg atgataggca
      301 aaggaccgcg ttccgacgag gtttgcaaag ctatcgtacg caacggctct gtatactttt
      361 gcgccgtagg cggtgccggt gcacttgcgt caaaatgcat aaagagctgt gaagttattg
      421 cattttcgga gcttggctgc gaatctgtca aaaagcttga attctgcgag tttccgctta
      481 ccgttgccat tgactgtcat ggaggaaatc tttttaaaca ataatatcgg agatataaaa
      541 cagatggatt ataacaactt tttgaatata agaaaaaaca taatcgagaa agagttttct
      601 cgaatgaatg atatgcaaaa aaaagcggtc tttagaataa aaggaccgct tttaattctc
      661 gcaggtgcag gttcgggaaa aacaaccgtg cttgttaatc gaatttcaaa tctgctcaaa
      721 tacggcgatg cttacaatac ggaaaaatct tcaaggactc cggatgagca cgatgttgag
      781 ctaatgcagc gctatcttgc aggtgacagc tctgtgtacc atgaaatacg tgatgtatta
      841 tcctatgacg ctcccatgcc gtggcaaatt ctcgctataa catttaccaa taaagccgca
      901 aacgagctta aagaacgcct cgttaaaatg ttaggggata atgctgcgga tatttgggca
      961 tgcacgtttc attctgcttg tttgcgtatt ctgagaagaa acggcgacaa actcgggttc
     1021 tcatctcatt ttacaatcta tgacaccgac gacagcaagc gcgtaatgaa agaatgtcag
     1081 aaattgctag gtgtt
//
LOCUS       DBMDVO010000624         1092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61813_length_1092_cov_1.051463, whole genome shotgun sequence.
ACCESSION   DBMDVO010000624 DBMDVO010000000
VERSION     DBMDVO010000624.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61813_length_1092_cov_1.051463"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>417
                     /locus_tag="ACZPE2_07985"
     CDS             <1..>417
                     /locus_tag="ACZPE2_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR1319825.1"
                     /translation="VDVKKQFPDAGYNKTRTLFWSDGWTGYSWNTELFPDYRGFLNFL
                     HEQGLKVTLNLHPALGIRSFENCYDAAARAMGLDPAEKKPIEFDITDTQFINTYFDTA
                     HHPYEDEGVDFWWIDWQQGKKSKLPGLDPLWALNHYH"
     assembly_gap    418..427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <428..>1092
                     /locus_tag="ACZPE2_07990"
     CDS             <428..>1092
                     /locus_tag="ACZPE2_07990"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1319826.1"
                     /translation="GKRPLILSRYAGIGSHRYPLGFSGDTFMSWRVLKFQPYFTSTAS
                     NCGYTWWSHDIGGHCFGRHDDEMYVRWVQLGTFSPIMRLHSSNDPLSGKEPWNYGWEA
                     EMTAERYLRLRHELIPYLYSMNYRTHKFGRALCEPLYYDNPECEGAYKCRNGYMFGSE
                     LLVCPVTSKIDKKTRRAETRVWLPKGRWTDVFTGKIYRGSKTVRIHSDLNTMPVFARE
                     GAI"
BASE COUNT          280 a          244 c          285 g          273 t
ORIGIN      
        1 gtagatgtta aaaagcaatt tccggatgca ggatacaata aaacgagaac gctgttttgg
       61 tcagacggat ggacaggata cagctggaac accgagcttt tccccgatta caggggcttt
      121 ttgaatttcc ttcatgagca ggggcttaaa gttacgctga atttgcatcc tgctctcggc
      181 atacgttcgt ttgaaaactg ctacgacgcg gcggcgaggg ctatggggct tgacccagcc
      241 gagaaaaagc cgatagaatt tgatataacc gatacacagt ttataaacac atattttgat
      301 acggcacacc atccgtatga ggatgaggga gtcgattttt ggtggataga ttggcagcag
      361 ggcaaaaaga gcaagcttcc gggtcttgac cctttatggg cgctgaatca ctatcatnnn
      421 nnnnnnnggg caagcgtcct ctcattctct cgcgttatgc cggcataggc tctcacagat
      481 atcccctcgg attttcggga gatacattta tgagctggcg agttcttaaa tttcagccgt
      541 attttacatc taccgcgtcg aactgcggat atacctggtg gagccatgat ataggcggcc
      601 attgcttcgg caggcacgat gatgagatgt acgtgcgctg ggtgcaatta ggcacttttt
      661 cgccgattat gcgtttgcat tcaagcaatg acccgctctc cggcaaggaa ccgtggaatt
      721 acggctggga agccgaaatg acagcggaac gttatttgag gcttcgtcat gagctgatcc
      781 cgtatctcta ctctatgaat taccgtactc ataaattcgg aagagcactc tgtgaaccgc
      841 tttactacga taacccggag tgcgagggtg cttacaaatg ccgtaacgga tatatgttcg
      901 gcagcgagct tttggtttgc cccgtcacgt caaagataga taaaaagacg cgccgcgccg
      961 aaacacgtgt atggctgcct aagggacgtt ggactgacgt cttcacgggc aaaatatatc
     1021 gtggttcaaa gacggtcaga atacacagcg accttaatac gatgcctgtt tttgcacgcg
     1081 agggagcgat aa
//
LOCUS       DBMDVO010000625         1090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61942_length_1090_cov_1.106168, whole genome shotgun sequence.
ACCESSION   DBMDVO010000625 DBMDVO010000000
VERSION     DBMDVO010000625.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61942_length_1090_cov_1.106168"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="ACZPE2_07995"
     CDS             complement(<1..385)
                     /locus_tag="ACZPE2_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1319827.1"
                     /translation="MAKNDSVSLSVVKRLPRYYRFLGEIKKNGTVRISSGELSKRMGF
                     TASQIRQDLNCFGGFGQQGYGYNVEQLYKEIGSILGVDNHLKTILIGAGNLGHAIASH
                     MSFEARGFNLIGIFDKNEALAGKLVR"
     gene            complement(399..>1090)
                     /locus_tag="ACZPE2_08000"
     CDS             complement(399..>1090)
                     /locus_tag="ACZPE2_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR1319828.1"
                     /translation="RENTEGAYAVGSKGINVDDDLAFDFVVATSEGSERIAKMAYEYA
                     RKNHKDRVSIINKANVIKTTDGKFLNICKNIGKEYPEITTDEWYIDITTAKLIDEKRR
                     KDFKVFVLPNLYGDIITDEAAEFQGGVGTAGSANLGKRYAMFEAIHGSAPRMISEGRG
                     QYADPCSMLRATVMLLSHIGKQDKADLLEKALDYCMFTDKKYVITGREDGATCSEFGD
                     YVMETVQKLSK"
BASE COUNT          296 a          249 c          203 g          342 t
ORIGIN      
        1 tacgtacaag cttgcctgca agtgcttcgt ttttatcaaa aataccgatg aggttaaacc
       61 cgcgtgcttc aaaagacata tgagaagcta tggcgtgacc gagatttcct gcacctatta
      121 gtatagtctt aagatgattg tcaacaccga gaatagaacc tatttctttg tacagctgtt
      181 caacattata tccgtatcct tgctgaccga agccaccgaa gcaattaaga tcctggcgaa
      241 tttgtgaagc ggtaaaaccc attctttttg ataactcacc ggatgatata cgaactgttc
      301 cgttcttttt tatttcacct aaaaagcgat aatatctcgg aagtctttta acgacagata
      361 acgataccga atcatttttt gccatttctc aattcacctc actttgaaag cttttgaacg
      421 gtttccatta cataatcgcc gaattcgctg cacgttgcac cgtcttctcg tccggtaatt
      481 acatattttt tatcggtgaa catacagtaa tccagagcct tttcaagaag atcggcttta
      541 tcttgcttcc cgatatgaga cagcaacata acagtagcac gaagcatgga gcaaggatcg
      601 gcatattgac ctcttccttc ggaaatcata cgcggagcag aaccgtgaat agcttcgaac
      661 attgcatatc ttttcccgag attagcactt ccggccgtgc cgacgccgcc ttggaattcg
      721 gcagcttcgt cggttataat atcgccgtat agattcggga gaacaaaaac cttaaagtct
      781 ttgcgtcgct tttcatcaat aagctttgct gtggttatgt cgatatacca ttcatcagta
      841 gtgatttcag ggtactcctt gccgatattt ttgcatatgt tgaggaattt accgtctgtc
      901 gttttaataa cattagcttt gttgataatt gatacgcgat ctttgtgatt cttacgagca
      961 tactcatacg ccattttggc aattctttcc gaaccttcac ttgttgctac aacaaaatca
     1021 aatgcaagat cgtcgtcaac atttattccc tttgaaccta cggcataagc accctcggta
     1081 ttttcacgga
//
LOCUS       DBMDVO010000626         1090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_61944_length_1090_cov_1.073812, whole genome shotgun sequence.
ACCESSION   DBMDVO010000626 DBMDVO010000000
VERSION     DBMDVO010000626.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61944_length_1090_cov_1.073812"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="ACZPE2_08005"
     CDS             <1..208
                     /locus_tag="ACZPE2_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319829.1"
                     /translation="AASFQETTRVLTDAAIKGKTDPLIGLKENVIIGKLLPSGTGMKC
                     YSEVELEKTYSDDTNPDEPANEPA"
     gene            353..778
                     /gene="rpsL"
                     /locus_tag="ACZPE2_08010"
     CDS             353..778
                     /gene="rpsL"
                     /locus_tag="ACZPE2_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1319830.1"
                     /translation="MPTFNQLVRSGRQTVEKKSKAPALGKGLNSKKNVMTNNASPQKR
                     GVCTVVKTDTPKKPNSALRKLARVRLTNGIEVSAYIPGVGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTLDTQGVTGRMQSRSKYGAKRPKAAKKA"
     gene            935..>1090
                     /locus_tag="ACZPE2_08015"
     CDS             935..>1090
                     /locus_tag="ACZPE2_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295349.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR1319831.1"
                     /translation="MPRRGQIAKRDVLPDPLYNSKLVTRLINSVMYDGKKGVAQKIVY
                     DAFNIIAE"
BASE COUNT          322 a          225 c          257 g          286 t
ORIGIN      
        1 ggcggcttcc ttccaggaaa ccacaagagt tctcaccgat gcagctatta agggcaaaac
       61 cgatcctctt atcggcctta aagaaaatgt tattatcggt aagcttcttc cgtccggcac
      121 gggtatgaag tgttacagcg aggtcgagct cgaaaagact tattcggatg atactaatcc
      181 cgatgagcct gcaaatgagc ccgcgtaaaa gaaaatttaa ataaaagctt gacaatcaaa
      241 ggttttaaat gttaaaatct ttgattgtgt gagcaaaaca aacacttttt taacggaagt
      301 gtttgttttg ctgacacatt gtgtcatatt ttcagaagga ggtgcaatga atttgccgac
      361 atttaaccag cttgttcgca gtggcagaca gacagttgag aagaaatcga aggctccggc
      421 tctcggtaag ggtcttaact cgaagaagaa cgttatgact aacaacgctt ctccgcagaa
      481 gagaggagtt tgcacggttg taaagaccga cactcctaag aagcctaact cagctctccg
      541 taagctcgcc agagttcgtc ttacaaacgg aatcgaagta tcagcttata ttcccggcgt
      601 aggtcacaat cttcaggagc actcagttgt tcttataaga ggcgggcgtg ttaaggatct
      661 ccccggtgtt cgttaccata ttgtccgcgg tacacttgat actcagggcg ttacgggcag
      721 aatgcagagc cgttcaaagt acggtgctaa gagacccaag gcagctaaaa aggcgtaatt
      781 tcgccgacta tttaaagaca gaatctactt gcaaggtttt aagtccgtgc agggtgtata
      841 tatatttctt atattatgcc tcttgcattg acaccacggg gttttgtcac cgagtaccta
      901 tgatatcatt ataattatga aggagggaag cgaagtgcca agaagaggcc agatagcaaa
      961 acgtgatgtt ttgccggatc cgctttacaa ttcaaagctt gtaaccaggc ttatcaacag
     1021 cgttatgtat gacggtaaaa agggcgtagc ccagaaaatc gtttatgacg catttaacat
     1081 tatcgctgag
//
LOCUS       DBMDVO010000627         1089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62043_length_1089_cov_0.921053, whole genome shotgun sequence.
ACCESSION   DBMDVO010000627 DBMDVO010000000
VERSION     DBMDVO010000627.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62043_length_1089_cov_0.921053"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="ACZPE2_08020"
     CDS             complement(<1..806)
                     /locus_tag="ACZPE2_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1319832.1"
                     /translation="MLDKMLKSPSVWEKLKDADLPIVCYGTGNGADKIFDEFERLGIK
                     ISAVMASDGFVRDRSFRGFKVKSLSDCENEFGDFYCVICFGSQLKSVTDNIKSVAAKH
                     KTFVPSVSVYGNGIANRDFFERNKSRICNIYSLLADEKSKDVFFKFVNFEYSGDLDIL
                     LSCESDKEEVFSDILRLGKNENYLDLGAYNGDTIKEFLHFSGNEYGSITAVEPAPRNF
                     KKLMEYTKSMSNITLVNKGIWSGYLTMGFSGKGGRNSYLLNAAQTKTDTVT"
BASE COUNT          317 a          210 c          198 g          364 t
ORIGIN      
        1 gtaacagtat ctgtctttgt ttgtgcggca tttaaaagat atgaatttct gccgcctttt
       61 cccgaaaaac ccatagtcaa atatccgctc caaatgccct tgttcacaag agttatattg
      121 ctcatacttt ttgtgtattc cataagcttt ttaaaatttc gcggagcagg ttcgacagct
      181 gttatactgc cgtattcgtt gccggagaaa tgcaaaaatt cctttattgt gtcgccgtta
      241 taagcaccga gatcaaggta attttcgttt ttgccgagcc ttaatatatc gctaaacact
      301 tcttctttat cgctctcaca ggatagaagt atgtctaagt ctccgctgta ttcaaaattg
      361 acaaacttaa aaaacacatc tttggatttt tcgtctgcaa gcagcgaata aatgttacag
      421 atgcggcttt tgttgcgttc aaaaaagtct ctgtttgcta tgccgtttcc gtaaaccgac
      481 acgctcggta caaaagtttt gtgctttgcc gcaacgcttt taatgttatc ggtgaccgat
      541 ttaagctgag aaccgaagca tattacacag taaaaatcgc cgaattcatt ttcgcagtca
      601 gagagacttt taactttaaa tccgcggaag cttctgtccc tgacaaatcc gtcacttgcc
      661 ataacggcag atatttttat acccagtctt tcaaattcgt caaatatttt atcggcgccg
      721 tttccggttc cgtagcaaac tatcggaagg tcggcgtctt ttaatttttc ccatacagaa
      781 ggagatttca gcattttatc aagcattata accactcttt ttatttctgc aaaagttcgg
      841 tattaattgt aacatataac gtgagttttc acaaatgttg ttatttactt tgataaaaaa
      901 agatattata atataagtga tgaattctaa ggaggtatta ttatgaacga tattaactct
      961 ctttataaat tatggtgcga aaatgccgtg aatgataaag accttattcc ggagcttaaa
     1021 tcagttgagg gaaatcagga tgagattctc gacagatttt ataaaaatct tgaattcggt
     1081 acggcaggt
//
LOCUS       DBMDVO010000628         1087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62189_length_1087_cov_0.959432, whole genome shotgun sequence.
ACCESSION   DBMDVO010000628 DBMDVO010000000
VERSION     DBMDVO010000628.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62189_length_1087_cov_0.959432"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            15..>1087
                     /locus_tag="ACZPE2_08025"
     CDS             15..>1087
                     /locus_tag="ACZPE2_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1319833.1"
                     /translation="MKYRENLRNVAIIAHVDHGKTTLVDQLLRQSGTFRDNEVVQERV
                     MDSNDIERERGITILSKNTSVHYKGIKINIVDTPGHADFGGEVERILTMVDGVLLLVD
                     AFEGCMPQTRFVLKKALALNKKVVVVVNKIDRPGARPAEVIDEVLDLFIELGANDDQL
                     EFPVIYASAKDGYASHNPDDRSGDMRPLFEEIISEIQPPEGDVDDPLQCLFSSLDYDD
                     YIGRIGVGRVQRGRITVNDKVVLCRTDGTQENVKISRLYQFEGLKRVEVNEAAAGDII
                     CVSGIADLNIGETICDPECVEPLPFVKIDEPTLSMNFMVNDSPFAGREGKFVTSRNLR
                     DRLFKEVETNVSMKVEETDSTDC"
BASE COUNT          314 a          191 c          269 g          313 t
ORIGIN      
        1 tgagggtgaa tagtttgaaa tacagagaaa atctcagaaa cgtagccatt atagctcacg
       61 ttgaccacgg taaaactaca cttgtggatc agctcttaag acagagcggt acatttaggg
      121 ataatgaggt ggttcaggaa cgcgttatgg attcaaatga tattgaacgc gagcgcggaa
      181 ttaccattct ttccaaaaat acatctgttc attataaagg aataaaaatc aatattgtcg
      241 atactcccgg tcatgccgat ttcggcggtg aggttgaacg tatactcaca atggttgacg
      301 gtgttctttt gcttgttgat gcctttgagg gctgtatgcc gcagacaagg tttgttttaa
      361 aaaaggcact tgcacttaat aaaaaagtag tagttgttgt taataagatt gacaggccgg
      421 gggctcgtcc tgccgaagtt atagacgagg tccttgattt gtttattgaa ctcggcgcga
      481 atgacgatca gcttgagttc ccggttatat atgcttctgc taaagatgga tatgcatcgc
      541 ataatcctga tgacagaagc ggtgatatgc gaccgctttt tgaagaaatt atcagtgaaa
      601 ttcagccgcc tgagggcgat gttgacgatc cgcttcagtg tcttttctca agcttggatt
      661 atgatgatta tatcggacgt ataggtgtag gcagagtcca gcgcggcaga ataacggtta
      721 atgataaagt cgtgctctgc agaaccgacg gtacgcagga gaatgtaaaa atctctcgcc
      781 tttatcagtt tgaaggcctt aaaagagttg aagttaatga ggctgctgca ggagatatta
      841 tttgtgtttc cggtatagcg gacctcaata tcggggaaac tatatgtgac cccgaatgtg
      901 tagaaccatt gccttttgtt aaaatagacg agcctactct ttcaatgaac tttatggtaa
      961 atgacagtcc gtttgcagga agagaaggaa aatttgtcac ttctcgcaat cttcgtgaca
     1021 gattgtttaa agaagttgaa actaatgtca gcatgaaggt tgaggagact gattccacag
     1081 actgctt
//
LOCUS       DBMDVO010000629         1082 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62563_length_1082_cov_1.405708, whole genome shotgun sequence.
ACCESSION   DBMDVO010000629 DBMDVO010000000
VERSION     DBMDVO010000629.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62563_length_1082_cov_1.405708"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            188..>1082
                     /locus_tag="ACZPE2_08030"
     CDS             188..>1082
                     /locus_tag="ACZPE2_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319834.1"
                     /translation="MSNLKTDVLIIGAGPSGIFTALEMLKNGSKKKIMIVEKGLPVEK
                     RHCPKAKTGKCVNCKPDCNITTGFSGAGAFSDGKLSLSCEVGGNLPDLIGHEFAQQMI
                     EYTDGIYLSFGADKKVEGVGHDEKIKAIRLRAIQAGLKLVDCPIRHLGTEMAQELYGK
                     IEKHLTDNGVEIIFKTECVDLIVEDGRAKGAVFFPCGKPESEKFSVSADNVVIATGRK
                     GADWLEDMCKKHNIEHLPGTVDIGVRVEVRNEVMEDVNEALYESKLIGYPEPFTNKVR
                     TFCQNPGGFVSQENYDNNSLAV"
BASE COUNT          311 a          227 c          287 g          257 t
ORIGIN      
        1 acctaactga taaaacctat gcaaaattca tatctgcgcc gcacaacgct cagctttgcg
       61 cggcgttttt ttgtccgctt gacattacgg ctgagtcgtg ctaatataat atgtaattag
      121 tagtaactga ttaccgtaaa tagccgaaaa acgtaagtcg gtatttacgg tttttttatg
      181 taaaggaatg agtaatttga aaacggacgt attgatcatc ggcgcaggcc cctcgggtat
      241 ttttaccgct cttgagatgc tgaaaaacgg ttcaaagaaa aaaataatga ttgttgaaaa
      301 aggcttgccc gtggaaaaga ggcattgtcc caaagccaag acgggtaaat gcgtaaactg
      361 taagcccgac tgcaatatca ctacgggctt ttccggtgcg ggtgcattct cagacggcaa
      421 gctttcgctc agctgcgagg tcggcggaaa tttgccggac cttatcgggc atgagttcgc
      481 tcagcagatg atagagtata cggacggaat atatctttcg ttcggtgccg ataaaaaggt
      541 cgagggcgta ggtcacgacg aaaaaatcaa ggcgataaga ctgcgtgcga tacaggcagg
      601 attgaagctt gttgattgtc ccatacgtca tctcggcacg gaaatggcac aggagcttta
      661 cgggaaaatc gaaaagcatc ttaccgacaa cggcgttgag attatcttta aaacggaatg
      721 cgtggatttg attgtcgagg acggcagagc aaaaggcgcg gtattctttc cgtgcggtaa
      781 gccggagagc gaaaaatttt ccgtttccgc cgacaacgta gtcatagcaa cgggcagaaa
      841 gggtgccgac tggcttgagg atatgtgcaa aaagcataat atcgagcatc tccccggaac
      901 cgttgacatc ggcgtcagag tcgaggtcag aaacgaggta atggaggacg taaatgaggc
      961 tctgtatgag tcaaagctta taggctatcc cgaaccgttc acaaataagg tgcgtacatt
     1021 ctgccaaaat cccggcggct ttgtcagcca ggagaattac gacaacaact cgcttgccgt
     1081 gg
//
LOCUS       DBMDVO010000630         1082 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62569_length_1082_cov_1.354740, whole genome shotgun sequence.
ACCESSION   DBMDVO010000630 DBMDVO010000000
VERSION     DBMDVO010000630.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62569_length_1082_cov_1.354740"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="ACZPE2_08035"
     CDS             complement(<1..312)
                     /locus_tag="ACZPE2_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319835.1"
                     /translation="MNRKNAGAVFGTLCGLTALTAMSAEIFYDFSINTKSSIHMSKLN
                     NLVQKVTNTSGSEEDASEKYSGLTGTPEMKKWYNEHGEDVYIMSDSLRLHGKIFKNNS
                     SK"
     gene            540..>1082
                     /locus_tag="ACZPE2_08040"
     CDS             540..>1082
                     /locus_tag="ACZPE2_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1319836.1"
                     /translation="MKITYINHSGFLVETENCYYVFDYYKGEMPRLDKSKEAIVFCSH
                     FHQDHFNPKIFEILDDMGMNYQAVLAKDIGKKKYPAGVKVTTAYHDQTYILDNGTKVS
                     TLLSTDSGVAFIVETSEGLIYHAGDLNDWHWEGETEDDNRQMASTYRAEIDKIKDAHF
                     DAAFVPLDPRHEEHYADGMLY"
BASE COUNT          299 a          216 c          232 g          335 t
ORIGIN      
        1 ttttgaactg ttgtttttaa atatcttacc gtgcagacga agagagtcgg acataatgta
       61 aacatcctca ccgtgctcat tgtaccattt tttcatttct ggcgtaccgg tgagtccgct
      121 gtatttttcg ctcgcgtctt cttcgctgcc ggatgtgttc gtgacctttt gaacgagatt
      181 gttgagttta ctcatatgta tagatgattt tgtgtttatc gagaaatcat agaatatttc
      241 agccgacatt gcggtcaatg ccgtcagtcc gcagagagtg ccgaaaaccg cacctgcatt
      301 ttttctgttc atagaaattc ctccgtcatt ttttaaaatt ataccatact cacgaatttt
      361 gtttcaagtc ttttgctatt tatatatttt atacggcaat tagaactgtt ttttattaaa
      421 aaagcaaaga ggctcaagta gaccgtttca agcgtttagc ttcgtatcga aaatgttgtg
      481 cggtattgat tccgatttat cgctttgata taataagaca agagtaatag aggtggatta
      541 tgaaaataac atacattaac cacagcggct ttttggttga aaccgagaac tgctattatg
      601 tttttgatta ttacaaaggc gagatgccga ggcttgacaa aagtaaggaa gccatagtct
      661 tttgcagtca cttccatcaa gaccatttca acccgaaaat ctttgaaatt cttgatgata
      721 tgggcatgaa ttatcaggct gttttggcga aggacatcgg caaaaagaag tatcctgccg
      781 gtgtgaaagt tacgactgct taccacgatc aaacctacat tcttgacaac ggcacaaagg
      841 ttagtacgct tctttcaacc gacagcggtg ttgcctttat cgtcgagaca agcgagggct
      901 taatttacca cgcaggtgat ttgaacgatt ggcattggga gggtgagacg gaagatgaca
      961 atcgccaaat ggcctccacg taccgtgccg agattgacaa aatcaaggac gctcattttg
     1021 atgccgcatt cgttcccctt gacccaaggc atgaggaaca ctatgccgac ggtatgcttt
     1081 at
//
LOCUS       DBMDVO010000631         1080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62769_length_1080_cov_0.913177, whole genome shotgun sequence.
ACCESSION   DBMDVO010000631 DBMDVO010000000
VERSION     DBMDVO010000631.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62769_length_1080_cov_0.913177"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="ACZPE2_08045"
     CDS             complement(<1..455)
                     /locus_tag="ACZPE2_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319837.1"
                     /translation="MNSNKKIVIAAIVMLLAVFCVMSACKKNIANSGDGEVVVVTDEN
                     GVPITDENGEAVTVVLDTTIVDVTNENGELVYDEDGNVKTSVVYISKEVGIPVTDENG
                     KAVTNARGEVLTTMIIVPPTTGGPVVTELPVTDGNGNVVTKPGGETVTYT"
     gene            701..>1080
                     /locus_tag="ACZPE2_08050"
     CDS             701..>1080
                     /locus_tag="ACZPE2_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013499.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR1319838.1"
                     /translation="MAYRTHNCNELTFDDLNKTVSLAGWVDTIRDHGGVAFIDLRDQY
                     GVTQIVINNDELLEGVKKESVISVTGKVVKRDEDTVNKKIATGELEVRVNEVEILGPC
                     SETLPFDVETSTDTREDVRLTYRFL"
BASE COUNT          298 a          260 c          236 g          286 t
ORIGIN      
        1 gtgtatgtga ccgtttcgcc gcccggcttt gtaacgacat tcccgtttcc gtcggttacg
       61 ggaagctcgg taacgacggg tccgcccgtg gtaggcggca cgattatcat tgtggtcagc
      121 acttcgccgc gcgcattggt aaccgccttg ccgttctcgt ccgtaacggg gataccgact
      181 tcctttgaaa tatagacaac agaggtcttt acattaccgt cctcatcata gacaagctcg
      241 ccgttttcgt ttgttacatc aactatggtg gtatcaagca caacggttac cgcttcgccg
      301 ttttcgtcgg ttatcggcac gccgttttca tccgtgacta ccacaacctc gccgtcgccg
      361 gaatttgcga tgttcttttt gcaagccgac attacgcaaa atacggcaag cagcataacg
      421 atggcagcta tgactatctt tttatttgaa ttcatcgttt taaacctccg tgtcagatga
      481 agcacttcaa aagcacataa acatatacgt tcgtattgta tcatattgca taagcttttt
      541 caattcaaaa atcataatat ttgttacaat attatgtaat taaatactat acattatccg
      601 aacattatga tataatacga tactgtataa ttaggttttt tatgcagtac ggtgtccgct
      661 gcggcaacgc tgcaaaaatc tcggaaggat gtataaatca atggcttaca gaacacacaa
      721 ctgcaacgag cttacctttg acgatttaaa caaaaccgtc agccttgcag gatgggtaga
      781 caccattcgc gatcacggcg gagtagcatt catcgacctt cgtgaccaat acggcgttac
      841 ccaaatagta ataaacaacg acgagctttt agagggcgta aaaaaggaaa gcgttatctc
      901 ggttacggga aaagtcgtaa agcgcgacga ggacaccgtt aacaaaaaga ttgccacggg
      961 cgagcttgag gtgcgtgtaa acgaggtcga aattctcggc ccgtgctccg aaacactccc
     1021 ctttgacgtt gagacctcaa ccgacacaag agaggacgta agactgacat accgtttcct
//
LOCUS       DBMDVO010000632         1079 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62837_length_1079_cov_0.838446, whole genome shotgun sequence.
ACCESSION   DBMDVO010000632 DBMDVO010000000
VERSION     DBMDVO010000632.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62837_length_1079_cov_0.838446"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="ACZPE2_08055"
     CDS             <1..1065
                     /locus_tag="ACZPE2_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HIR1319839.1"
                     /translation="FDELNIPKPSVTPKNGYTTWYNYYSKINEKIVSDDLEALSKVKS
                     DIDIFQIDDGYQSATGDWLSIDNKKFPNGMKSVADKIHSKGMLAGLWLAPFGAEFTSK
                     TATQHHDWLIRKKNGHPVTCGINWGGFYALDIEVPEVKNYIKHFFDVILNDWGFDLVK
                     LDFLYAAAIIPNHGKSRGQLMCEAMDFLRECVGEKMILGCGVPLMPAFGKVDYCRIGA
                     DMGLRWKVPFFSNREFISTYHTLGNSIFRRQLDGRAFLNDPDVFLLRDENIHCTFEQR
                     KIIATVNKVFGSVLFTSDNVGKYSDEQMSVLLDTFKKSKIDVKSAEFLNENKRIMKFV
                     YTQDGIEHTFKFNIDKGTIV"
BASE COUNT          338 a          222 c          232 g          287 t
ORIGIN      
        1 tttgacgagc ttaatatacc caagccttct gtgaccccca aaaacggata taccacatgg
       61 tataactatt acagcaaaat taatgaaaaa atagtatctg acgacctcga ggcgttatca
      121 aaggtgaaat ccgatatcga tatatttcag atagacgacg gatatcaatc ggcaaccggt
      181 gattggctct ctatagacaa taagaaattc cctaacggta tgaaatctgt tgccgacaag
      241 attcattcaa aaggaatgct tgcaggtctt tggttggctc ctttcggcgc agagttcact
      301 tctaaaaccg ctacgcagca ccacgattgg ctcattcgca agaaaaacgg tcatccggta
      361 acttgcggaa ttaactgggg aggcttttac gccctggaca ttgaagttcc ggaggttaaa
      421 aactatatca aacacttttt cgatgtcatt ctgaatgatt ggggatttga cttagttaag
      481 cttgactttt tatatgctgc cgcaataata ccgaatcacg gcaaaagccg aggccagctt
      541 atgtgtgagg ctatggattt tctgcgcgaa tgtgtcggcg aaaaaatgat actcggttgc
      601 ggcgttccat tgatgccggc attcggcaaa gtagattatt gccgtatcgg tgcggatatg
      661 gggcttagat ggaaagttcc gttcttttct aacagagaat tcatctcaac gtaccacacc
      721 ctcggcaatt ccatattccg ccggcagctt gacggcagag catttctcaa cgacccggac
      781 gttttcctct tgagagacga aaacatacat tgcacgtttg agcaacgcaa gataatagca
      841 actgtaaaca aggttttcgg cagcgtttta tttacatctg ataatgtcgg aaaatatagc
      901 gatgagcaaa tgtcagtact tctcgacaca tttaaaaaga gcaaaatcga tgttaaaagc
      961 gccgagtttt taaatgagaa caaacgaata atgaaattcg tatacacaca ggacggtatt
     1021 gaacacacct ttaaatttaa tattgataaa ggcacaatag tataaaaagg aatccgcag
//
LOCUS       DBMDVO010000633         1078 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62896_length_1078_cov_0.993859, whole genome shotgun sequence.
ACCESSION   DBMDVO010000633 DBMDVO010000000
VERSION     DBMDVO010000633.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62896_length_1078_cov_0.993859"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="ACZPE2_08060"
     CDS             complement(<1..324)
                     /locus_tag="ACZPE2_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1319840.1"
                     /translation="MSFIAGTGKTNVDILYAGLKRLPQLGEELYSDGFKLCLGGGVPG
                     TMVNLGRLNIPVRTATWLGLDMFSDFASNEYRDNSVEITNLYEGGVLPLNITSAMITP
                     EDRTFV"
     gene            540..>738
                     /locus_tag="ACZPE2_08065"
     CDS             540..>738
                     /locus_tag="ACZPE2_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319841.1"
                     /translation="MDLKPLIDKKDEAAKYMIDEITHICKDMDTRSPGTKGEKEACEY
                     MAKVLKEDCGCERADVESFKEN"
     assembly_gap    739..748
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <749..>1078
                     /locus_tag="ACZPE2_08070"
     CDS             <749..>1078
                     /locus_tag="ACZPE2_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319842.1"
                     /translation="LLAIALYFVAPIISLILIVAGLAIVFLQFGLYKKCVDRFFPELT
                     GHNVTAVKKCTGEVKRRIFFNGHPDAAWEWPVNYALGGIGFEGHAVICALGAVYYMVI
                     SIIYMAQ"
BASE COUNT          307 a          225 c          229 g          307 t
ORIGIN      
        1 tacaaatgta cggtcctccg gagttatcat tgccgacgtt atgttaagcg gaagtacacc
       61 gccttcataa aggtttgtga tttccacgga gttgtcgcgg tactcattgg acgcaaaatc
      121 cgaaaacata tcaagaccga gccacgtggc ggttctaacc ggaatgttca gtctgccgag
      181 attcaccatt gtaccgggga ctcctccgcc gagacacagc ttgaaaccgt ctgaataaag
      241 ctcttcgccc aactgcggca gccttttaag acccgcataa agaatatcga cgtttgtttt
      301 accggtaccg gctataaaag acattcgttt ttcatctcac ttgctttgaa aatatatttt
      361 attttgttat catacaacat tttgtctcat aagtcgaggc ataaaatgca taaattgaca
      421 gatcacaaca ttgtattgta ctacgtgaat ataaaaaatc gtttcaaagg taatttttat
      481 tgactttgca cagtttttat aataaaatat tagatatact tttgagaagg agagttcata
      541 tggatttaaa accgcttatt gacaaaaaag acgaagcagc aaagtacatg attgacgaaa
      601 tcacgcatat ctgtaaggat atggataccc gcagccccgg cactaaggga gagaaagagg
      661 cttgcgagta tatggcaaaa gttcttaaag aggattgcgg ctgcgaacgt gcggacgttg
      721 aatcgtttaa agaaaaccnn nnnnnnnntc ttttggcaat cgcgctttac tttgtggcac
      781 cgattatttc gcttattctc attgttgcag gtcttgcaat agtattttta cagttcggcc
      841 tttacaaaaa atgcgttgac cgtttcttcc ccgagcttac cggtcacaat gttaccgcag
      901 ttaaaaagtg caccggcgaa gtaaaacgcc gcatattctt taacggacat ccggacgctg
      961 cttgggagtg gcctgtaaac tatgcgctcg gcggcatagg ctttgagggt catgccgtaa
     1021 tctgcgccct cggcgcagtt tattatatgg taatatccat tatttatatg gcccaaaa
//
LOCUS       DBMDVO010000634         1077 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_62948_length_1077_cov_1.399590, whole genome shotgun sequence.
ACCESSION   DBMDVO010000634 DBMDVO010000000
VERSION     DBMDVO010000634.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62948_length_1077_cov_1.399590"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="ACZPE2_08075"
     CDS             <1..293
                     /locus_tag="ACZPE2_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1319843.1"
                     /translation="GPRGSRVNKIVELLGGEKIDIVNYSEDPKQFVAAALAPANVLSV
                     EIPDSEVKSCVVTVPDNQLSLAIGNKGQNARLAARLTGWKIDIKPESGFYEG"
     gene            321..590
                     /gene="rnpM"
                     /locus_tag="ACZPE2_08080"
                     /gene_synonym="ylxR"
     CDS             321..590
                     /gene="rnpM"
                     /locus_tag="ACZPE2_08080"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1319844.1"
                     /translation="MKQKKIPLRKCLGCGCQKPKQELVRVVRSAEGEVSLDLTGKKAG
                     RGAYICKSAQCLKAARKAKRIDRAFSVTVPDDVYDEMEKELSENE"
     gene            583..900
                     /locus_tag="ACZPE2_08085"
     CDS             583..900
                     /locus_tag="ACZPE2_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013417.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR1319845.1"
                     /translation="MNDRALGLLGIARRASALSLGHDAALSSVKEGKASVCILASDSS
                     ERLKDEFISLCEKTGGKTPALTLGCTMNDLGRCLGAKMTAVLTVNDGGFARKITELIR
                     EGK"
BASE COUNT          321 a          237 c          300 g          219 t
ORIGIN      
        1 taggtccgag aggttcgaga gtcaacaaga tagtagagct gctcggcggc gaaaagatag
       61 acatagtcaa ttacagcgaa gaccccaagc agtttgttgc ggctgctctc gctcccgcaa
      121 acgtacttag cgtagagata cccgattcgg aggttaaatc ctgcgtagtt accgtaccgg
      181 acaatcagct ctcgctcgca ataggcaaca agggtcagaa cgcacgtctc gcggcgcgcc
      241 ttaccggctg gaagatagat ataaaacccg aaagcggatt ttacgaaggc tgagtctttt
      301 gatgtttaag ggagaataaa atgaagcaga aaaaaatacc tctgagaaaa tgtctcggat
      361 gcggctgcca aaagcctaag caggagcttg tgagagtggt cagaagcgcc gagggcgagg
      421 tctcgcttga cctcaccggc aaaaaggcgg gcaggggagc gtatatatgc aaaagcgcac
      481 agtgcctcaa ggcggcgcgc aaagcaaagc ggatagaccg cgcgttctcg gtgacggtac
      541 ccgacgatgt ttatgacgag atggaaaagg agctttcgga aaatgaatga cagggcatta
      601 gggctgctcg gtatagcaag gcgggcttcg gctttgagcc tcggtcatga cgcggcactg
      661 tcctccgtaa aagaaggcaa agcctcagtg tgcattctgg cctccgattc gtccgaaaga
      721 ctcaaggacg aatttatctc cctttgcgaa aagaccggcg gcaaaactcc ggcattaacc
      781 cttggctgta caatgaacga tttaggcagg tgcctcggcg ccaagatgac cgcggtatta
      841 acggtcaacg acggcggctt cgcacgtaaa ataacagaat tgatccggga gggaaagtaa
      901 tatatgacaa aaaaatataa gatacatgaa gcagctaaag attttgatat tcagagcaag
      961 gatttgatag agcttgtctc aaaatacttt gacggcgaga aaaaatatca gaccgcgctc
     1021 gaagagaaag aacttgattt ggtttttgat ttgctctcaa agcagaatgc gccgaag
//
LOCUS       DBMDVO010000635         1075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63117_length_1075_cov_0.903491, whole genome shotgun sequence.
ACCESSION   DBMDVO010000635 DBMDVO010000000
VERSION     DBMDVO010000635.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63117_length_1075_cov_0.903491"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1075
                     /locus_tag="ACZPE2_08090"
     CDS             <1..>1075
                     /locus_tag="ACZPE2_08090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1319846.1"
                     /translation="MPDGEYTVNDTVAKIKDKKGNETELTLLQKWPIRNPRPIKKRLP
                     ATKPLVTGQRVIDTVFPVAKGGAAAIPGGFGTGKTMTQHQLAKWCDADIIIYVGCGER
                     GNEMTQVLTEFSDITDPKTGRPLLDRTMLIANTSNMPVAAREASIYTGITIAEYYRDM
                     GYDTALMADSTSRWAEALREISGRLEEMPADEGFPAYLPSRLSEFYERSGYTENLNGT
                     EGSITLIGAVSPQGSDFSEPVTQNTKRFTRCFWALDKSLAYARHYPAINWNLSYSEYT
                     GDLANWYRDNVSPDFMKYREELCSLLLEENSLMEIVKLIGADVLPDDQKLVIEIARVI
                     RVGFLQQNAFHADDTFVPLEKQFK"
BASE COUNT          296 a          267 c          264 g          248 t
ORIGIN      
        1 aatgcccgac ggcgaataca ccgtaaacga tacggttgca aaaatcaagg acaaaaaggg
       61 taacgaaacg gagcttacgc ttttacagaa gtggcccata cgaaatccga gaccgattaa
      121 aaagagactc cccgccacta aaccgcttgt cacgggtcag cgtgtaatcg acacggtttt
      181 cccggtagca aagggcggtg cggcggcaat ccccggaggc ttcggtacag gtaaaacaat
      241 gactcagcat cagcttgcca aatggtgcga tgccgatatc ataatttatg tcggctgcgg
      301 cgagcgcggt aacgagatga ctcaggttct taccgagttt tcggatatta ccgaccccaa
      361 gacgggcaga ccgcttcttg acagaacaat gcttatagca aatacctcta acatgcccgt
      421 tgcggcacgt gaggcttcaa tttacacagg cattacaata gctgagtatt atcgcgatat
      481 gggctatgat acggctctta tggcagactc gacatcacgt tgggctgagg cgctcagaga
      541 aatttccgga cgacttgagg aaatgcccgc cgacgaaggc ttcccggcat atcttccgtc
      601 gagactttcc gaattctacg agcgttcagg ttataccgaa aaccttaacg gcactgaggg
      661 ctcaataacg cttatcggcg cggtatcgcc tcagggctcc gacttttccg agcctgtaac
      721 tcagaataca aagcgtttta caagatgctt ctgggctctt gataaatcgc tcgcttatgc
      781 gagacactat ccggcaataa actggaacct ctcttacagc gagtatacgg gtgaccttgc
      841 taactggtac agagataacg tctcacccga ctttatgaag taccgcgaag agctttgctc
      901 gcttttactt gaggaaaact cacttatgga gatagtaaag ctgataggtg cggacgtgct
      961 tcccgacgat cagaagcttg taattgaaat tgcaagagta atcagagtcg gattccttca
     1021 acaaaacgca ttccatgccg acgatacctt cgttccgctt gagaagcagt ttaag
//
LOCUS       DBMDVO010000636         1074 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63204_length_1074_cov_0.770812, whole genome shotgun sequence.
ACCESSION   DBMDVO010000636 DBMDVO010000000
VERSION     DBMDVO010000636.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63204_length_1074_cov_0.770812"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="ACZPE2_08095"
     CDS             <1..561
                     /locus_tag="ACZPE2_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017944.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR1319847.1"
                     /translation="EKLYKSGYTDIFCVPTHIMCGIEYDKACRMIEKFNDKMKIRISR
                     PLVTETSDYYNIVDIFKHRLTDKNRLYIFMGHGSDHFANAAYPALDYHFKNADMNNVF
                     VGTVEGFPDLETVISHAMKTDIKNVTLLPFMLVCGDHAKNDMAVEWKEEFENNGFNVN
                     CDLTGLGEIKEIQDMYAEHLRNVWGQ"
     gene            888..>1074
                     /locus_tag="ACZPE2_08100"
     CDS             888..>1074
                     /locus_tag="ACZPE2_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1319848.1"
                     /translation="MNNFIFENSTKVYFGKGSVKEFLAGSIKEYGNNVMLAYGGGSIK
                     KNGVYDEINSILKAEGKN"
BASE COUNT          383 a          186 c          205 g          300 t
ORIGIN      
        1 gaaaaattat ataaaagcgg ttacacagat attttttgtg tccctactca cattatgtgc
       61 ggtattgaat acgacaaagc ttgcagaatg attgagaaat ttaacgacaa aatgaaaatc
      121 agaatttctc gtccgcttgt taccgaaacg agtgactatt acaacattgt tgatattttc
      181 aaacacagac ttaccgacaa aaacagactt tacattttta tgggacacgg cagcgaccat
      241 tttgcgaatg ccgcctaccc tgctcttgat tatcacttta aaaatgccga tatgaacaac
      301 gtatttgtcg gtactgttga gggttttccc gaccttgaaa cagtcatctc ccacgcaatg
      361 aaaaccgata taaaaaatgt aacactactt ccgtttatgc ttgtatgcgg tgaccacgcg
      421 aaaaatgata tggctgtcga atggaaagaa gaatttgaaa ataacggttt taatgtaaac
      481 tgtgacctta ccggtcttgg cgaaattaaa gaaatacaag atatgtacgc cgagcattta
      541 agaaatgttt ggggacagta ataaaagagc ctgtcgacaa aatgtcgaca gctccaaaaa
      601 tcacatcata gatgtaattt ttgagtattt tcagagaaaa aatcataaag ttcctccgaa
      661 gtcgaaactt tagattttaa gacatttctg ccgcacatgt cgccagaaat gatttttaat
      721 gaggttaaca cctcaaactt cccgaaataa agtagacaaa accgtaatgg ttttgtctac
      781 aattttaagc acaaaccttt tggtttacac tgtccaacaa atagatactt ctgtttaaca
      841 aaaggttatg ttacaataaa tttaaagaaa gggtacggtg attgtttatg aataatttta
      901 tatttgaaaa cagcacaaag gtatatttcg gcaaaggcag cgtaaaggag tttcttgccg
      961 gaagtataaa ggaatacgga aacaatgtaa tgcttgcata cggcggcggt tcaatcaaaa
     1021 agaacggtgt atatgatgag ataaacagca tattaaaagc cgagggcaaa aacg
//
LOCUS       DBMDVO010000637         1073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63226_length_1073_cov_1.734568, whole genome shotgun sequence.
ACCESSION   DBMDVO010000637 DBMDVO010000000
VERSION     DBMDVO010000637.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63226_length_1073_cov_1.734568"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="ACZPE2_08105"
     CDS             <1..606
                     /locus_tag="ACZPE2_08105"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HIR1319849.1"
                     /translation="PQGVPISAIIFGGRRAATTPLVYQSFSWQHGTYVGSAVSSETTA
                     AATGAVGVLRHDPMAMKPFCGYHMADYWAHWLDMGKKLGDKAPKIFNVNWFKQDENGN
                     FIWPGFGDNMRVLDWIIKRCEGTIDAEKTPIGYLPKKGDINLKGIEDEVTSEVEDKLL
                     AVDRDLWKKEVEEMKEFYKQFGDKLPDGLKQELADLEARLG"
     gene            complement(792..>1073)
                     /locus_tag="ACZPE2_08110"
     CDS             complement(792..>1073)
                     /locus_tag="ACZPE2_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1319850.1"
                     /translation="IVLPMLKPALAVQAIFSFVASWNNYFLPSLIIDTSTKKTMPIMI
                     AQLRSADFLKFDMGQVYMFIFIAIIPVIIVYFCLSKFIIAGVSLGGVKE"
BASE COUNT          322 a          242 c          253 g          256 t
ORIGIN      
        1 ccccagggtg ttccgatctc agctatcatc ttcggcggca gacgtgcagc tacaactcct
       61 ctggtttacc agtcattcag ctggcagcac ggcacatatg taggctctgc agtttcttct
      121 gaaacaacag cagctgctac aggtgcagta ggcgtactcc gtcacgatcc tatggctatg
      181 aagcccttct gcggctacca catggctgat tactgggcac actggcttga tatgggcaag
      241 aagctcggcg acaaggctcc taagatcttc aacgttaact ggttcaagca ggacgagaac
      301 ggcaacttca tctggcccgg tttcggcgac aacatgagag ttctcgactg gatcatcaag
      361 agatgtgaag gtacaatcga cgctgaaaag acacctatcg gctaccttcc taagaagggt
      421 gacatcaacc tcaagggtat cgaggatgag gttacatcag aagttgaaga caagctgctc
      481 gcagttgaca gagacctctg gaagaaggaa gtagaagaga tgaaggaatt ctacaagcag
      541 ttcggtgaca agctccccga cggtttaaag caggaactcg ctgatctcga agcaagactt
      601 ggctaatcat aaccgaataa actccgatcc cctgttaagt gattaacagg ggattttctt
      661 atgcttcata cgctcctagc tgatatgaca gggtaaatac cgcactgttg caacggcatt
      721 atatacggca aaacggcacc ggaaaatttc cgatgccgtt tctgtataaa atatattaac
      781 ccaaaattgt cttattcctt tacaccaccg agtgaaacac cggcgatgat gaatttcgat
      841 aagcagaaat aaacgatgat aacaggtatg attgctatga aaatgaacat ataaacctga
      901 cccatatcaa actttaagaa gtctgctgaa cgaagctgtg caatcatgat aggcattgtc
      961 ttctttgttg atgtatcaat gatcaacgaa ggaaggaagt agttgttcca tgatgcaacg
     1021 aagctgaata ttgcctgtac tgcaagagca ggcttcagca taggaagaac gat
//
LOCUS       DBMDVO010000638         1069 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63559_length_1069_cov_1.185950, whole genome shotgun sequence.
ACCESSION   DBMDVO010000638 DBMDVO010000000
VERSION     DBMDVO010000638.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63559_length_1069_cov_1.185950"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="ACZPE2_08115"
     CDS             <1..336
                     /locus_tag="ACZPE2_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR1319851.1"
                     /translation="KLSLQAKNTIPITAQCTVFAESEVISLVGEGKPMDEIAAGIQLA
                     VAKRCFVMAKKAGATDSITLTGGCAKNEGLKRAIEHVLKIKVVDLPIDPQLMGALGAA
                     EYARQKGMV"
     gene            492..>1069
                     /locus_tag="ACZPE2_08120"
     CDS             492..>1069
                     /locus_tag="ACZPE2_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015555.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319852.1"
                     /translation="MKLYDKLIGQIKNLLPENAKRFSYSEPTLKIKKDNSILLLKETA
                     YELGGSQKPCVSTVAVSDSIKFDNCTYLYGKDIPEIRSDCCFGKIVFLEIKDIGEEQS
                     AFDKIKELERLKYSFCPEEFMTRASAFNMREQIRVSKKAVKNGVTFSDYGSSLIKAYL
                     EFPVVKSVQIIFITDFDCFNELGMICDKVRQTT"
BASE COUNT          345 a          184 c          234 g          306 t
ORIGIN      
        1 aaattgtctc tccaagcaaa aaatactatt cctataacgg cacagtgtac tgtatttgcc
       61 gaatcagagg taatttctct tgtaggcgag ggaaaaccca tggatgagat agcggcgggt
      121 attcaactcg cagttgccaa acgttgtttc gttatggcaa aaaaagcagg tgctaccgac
      181 agtataacgc ttaccggcgg ttgtgctaaa aacgaaggtc tcaagcgtgc tattgagcac
      241 gttctgaaaa ttaaggttgt tgaccttcca atcgaccctc agcttatggg cgcgcttggt
      301 gctgccgagt atgcaaggca aaaggggatg gtataaatga aagctttaat tataatttta
      361 attgttcttg cagcgctttt tgtgctcaca ttttgcatct attttttcaa tctcgatatg
      421 aaacttgttc gtaagatata tgatgcgctc ggcaaacatt atgattcgct tgaaaaggac
      481 aaaaaacttt aatgaaactt tatgacaagc ttatcgggca aataaaaaac cttttgcctg
      541 aaaacgccaa aaggttttct tatagtgaac cgacgcttaa aataaaaaaa gacaattcaa
      601 tacttttatt gaaagaaacg gcgtatgaac tcggcggaag tcaaaagccg tgtgtaagca
      661 cggtcgcggt gagcgacagt ataaagtttg acaattgcac atatctttac ggaaaagata
      721 ttccggaaat aaggtctgat tgctgtttcg gaaaaatagt ttttcttgaa atcaaagata
      781 ttggtgaaga acaatcggct tttgataaga taaaagaact cgaacgtctt aaatatagct
      841 tttgccctga ggaatttatg acaagagcat ctgcatttaa tatgcgggag cagataagag
      901 tcagcaaaaa agcggtaaaa aacggtgtga cattctcgga ctacggctca tcgcttatta
      961 aggcttatct tgaattccct gtggtgaaga gtgtgcagat aatatttatt accgatttcg
     1021 actgttttaa tgaactcggt atgatttgtg ataaagtaag gcaaacaac
//
LOCUS       DBMDVO010000639         1069 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63578_length_1069_cov_1.042355, whole genome shotgun sequence.
ACCESSION   DBMDVO010000639 DBMDVO010000000
VERSION     DBMDVO010000639.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63578_length_1069_cov_1.042355"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="ACZPE2_08125"
     CDS             complement(<1..487)
                     /locus_tag="ACZPE2_08125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1319853.1"
                     /translation="MSRIINKLIRKISGSELAQKQNASIIKNGKPNEKIIPFLRQTAA
                     DGCVLLKNNGALPINGKDNVALFGRVQNDSFFVGNGSGGDVCAPYKISFADGIEKNGK
                     IHINEDLRKIYNDWCGKRKNIPDPGFWGHWPRFYPEMPLKDNIVRSASEKSNKAVVFI
                     GR"
     gene            633..>1069
                     /locus_tag="ACZPE2_08130"
     CDS             633..>1069
                     /locus_tag="ACZPE2_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1319854.1"
                     /translation="MPHVSVLLKPASSACNMNCKYCFYKDEANCRETAFCGMMSLSDA
                     EEIIKSAAEYASGSCTFLFQGGEPTLAGLDFYKGFAEIEKKYTKKGLIFHHSIQTNGL
                     LIDDDWAKFFKKNNYLVGLSLDGPAQINDLNRVDVSGKGTFNRV"
BASE COUNT          269 a          237 c          231 g          332 t
ORIGIN      
        1 ttctgcctat aaataccacg gccttattgc tcttttcgga tgcacttctt acaatgttgt
       61 ctttaagcgg catttccgga taaaatctcg gccaatgtcc ccaaaacccc ggatccggta
      121 tgtttttgcg cttgccgcac caatcattat aaatttttct gagatcttcg tttatatgta
      181 tttttccgtt tttttctatg ccgtcggcaa agctgatttt ataaggcgcg catacgtctc
      241 caccagagcc gttgccgaca aaaaagctgt cgttttgcac tctgccgaac agcgcgacat
      301 tatccttgcc gtttatgggc aaagccccgt tgtttttcag caatacgcag ccgtccgccg
      361 ctgtctgtcg caaaaacggg attattttct cattcggctt gccgtttttt attatgctcg
      421 cattttgctt ttgcgcaagt tcactgccgc tgattttacg aatcagctta tttataatcc
      481 tgctcatttt acagctcctt ttttattttt atgtcataat atcacatact gattgttttg
      541 tcatcacgtt gtcatcccga agagaaaaat ttgagtttcc gagacgagtg tgctaatata
      601 ataaatatta cataaagctg cggaggtaga aaatgccaca tgtaagtgtt cttttaaagc
      661 cggcttcttc ggcgtgcaat atgaattgca aatactgttt ttataaggat gaggcaaatt
      721 gccgcgaaac tgctttttgc ggtatgatgt cgctgtcgga cgccgaggag ataattaaaa
      781 gtgccgcgga atacgcaagc ggcagctgta cgtttttgtt tcagggcgga gagcctacgc
      841 ttgcggggct tgatttttac aagggctttg cggaaatcga aaagaaatat acaaaaaagg
      901 ggctcatatt ccaccacagc atacagacca acggcctttt gattgatgac gactgggcaa
      961 aattctttaa aaagaacaac tatcttgtag gtttgtcgct tgacggcccg gcacagataa
     1021 acgacctcaa ccgcgtcgat gtgagcggca agggtacatt taacagggt
//
LOCUS       DBMDVO010000640         1068 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63683_length_1068_cov_1.033092, whole genome shotgun sequence.
ACCESSION   DBMDVO010000640 DBMDVO010000000
VERSION     DBMDVO010000640.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63683_length_1068_cov_1.033092"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1068
                     /locus_tag="ACZPE2_08135"
     CDS             <1..>1068
                     /locus_tag="ACZPE2_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR1319855.1"
                     /translation="KEETPARQFTIGINDDVTHLSLPEKPAPITAAEGTIECKFWGLG
                     GDGTVGANKDSIKIIGDHTDKYVQAYFQYDSKKTGGVTISHLRFGDKPIKSSYYINKA
                     DFVACHNPSYIEKGFRMVEDVKPGGVFLINCQWDEKELSEKLNAETKRYIAKNNIQLY
                     TVNAIDIAAKIGLGKRTNTVLQSAFFSLAKVLPAEEAIGYMKEKAQKFMKKGIEIVKM
                     NEAAIDAGASAYVKIDVPASWADAEDAPKAVTEHGPAKLVKMVDSVMKPVGNMDGDRL
                     PVSAFSEHVDGTFEQGASAYEKRGVAVMVPEWDGATCAQCNKCSYVCPHATIRPYALT
                     AEEAANAPAVATIVDKKGKGK"
BASE COUNT          284 a          272 c          274 g          238 t
ORIGIN      
        1 ttaaggagga gactcccgca cgtcagttca caatcggcat caacgatgat gttactcacc
       61 tttcacttcc cgaaaagccg gctcccatta ccgcggcaga gggtactatc gagtgcaagt
      121 tctggggtct cggcggcgac ggtacagtcg gtgcaaacaa agactctatc aagatcatcg
      181 gtgaccatac agataagtat gttcaggcat atttccagta tgactcaaag aagaccggcg
      241 gtgttacaat ttctcacctt cgcttcggcg acaagcccat taagagctca tactatataa
      301 acaaggccga cttcgttgcc tgccataacc cctcttacat agagaagggc ttcagaatgg
      361 tagaggacgt aaagccgggc ggcgtattcc tcatcaactg ccagtgggat gaaaaggagc
      421 tttccgaaaa actcaacgca gagactaaga gatacatcgc aaagaacaac attcagctct
      481 acaccgttaa cgctatcgac atagccgcta aaataggtct cggtaagcgt accaacaccg
      541 ttttgcagtc tgcattcttc tcgcttgcta aggttcttcc cgctgaggaa gcaatcggct
      601 acatgaaaga aaaagctcag aagtttatga agaagggcat tgagatagtt aagatgaacg
      661 aggcggctat cgacgcaggt gcttccgctt acgttaagat tgatgttccg gcttcttggg
      721 ctgacgcaga ggatgccccc aaagctgtta ccgagcacgg tccggcgaag cttgtcaaga
      781 tggttgacag cgtcatgaag cccgtaggca atatggacgg cgaccgtctc cccgtatccg
      841 cattctccga gcacgttgac ggcaccttcg agcagggcgc ttcggcttac gaaaaacgcg
      901 gcgttgccgt tatggttccc gagtgggacg gtgcgacttg tgctcagtgt aacaagtgct
      961 cttatgtctg cccgcacgct acaatccgtc cgtatgctct taccgctgag gaagcagcta
     1021 atgctccggc agttgcaaca atagttgaca agaaaggtaa aggcaagg
//
LOCUS       DBMDVO010000641         1067 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_63782_length_1067_cov_0.854037, whole genome shotgun sequence.
ACCESSION   DBMDVO010000641 DBMDVO010000000
VERSION     DBMDVO010000641.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63782_length_1067_cov_0.854037"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            128..>1067
                     /locus_tag="ACZPE2_08140"
     CDS             128..>1067
                     /locus_tag="ACZPE2_08140"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1319856.1"
                     /translation="MATKKLLIVESPAKAGTIKKYLGKDYTVMASMGHIIDLPKSQMG
                     IDFENDYTPKYITIRGKGQLLTQLKKEAKKADVVYLATDPDREGEAISWHLAEALKID
                     PESNCRVTFNEITKTAVKAAIKQPRKIDMDLVDAQQARRVLDRIVGYTISPILWEKVK
                     RGLSAGRVQSVATKLICDREEEIENFTPQEYWSIETVLNDPKSKKDFNAKYYGEKGKE
                     VKLNNAEETKKVSDAIANADFVIEKVKRGQQKRNPQPPFTTSTLQQDASRKYNFQAAK
                     TMQIAQTLYEGINLGGKIGTIGLITYMRTDSLRIADD"
BASE COUNT          403 a          179 c          251 g          234 t
ORIGIN      
        1 tcaagggaac tgaaaagaaa cgtaagcgaa ataaatacaa agcttataat gcttgaagtc
       61 aagggattgg ttaagaaact tcccggaagt aaatatcaac tgaaattata gatacggagg
      121 aaattatatg gcaacgaaaa agctgttaat agtggagtca ccggcgaaag cggggacaat
      181 aaaaaaatat cttggcaagg actatacagt tatggcgtct atggggcata taatcgacct
      241 tccgaaaagt caaatgggta tagactttga aaatgattac acaccgaaat atataacaat
      301 acgcggtaaa ggacagctgc ttacacaact gaaaaaagag gctaaaaaag cagatgtagt
      361 ttatctcgca actgacccgg accgcgaggg tgaggcaata agctggcatt tggcggaggc
      421 acttaaaata gaccccgagt caaattgccg tgtaacgttt aacgaaatta caaaaacagc
      481 cgttaaggcg gcaataaaac agccgagaaa gattgatatg gatcttgttg acgcacaaca
      541 ggcaagacgt gtgcttgaca gaattgtggg ttatacaatc agtcctattc tttgggaaaa
      601 ggttaaaaga ggacttagtg ccggtcgtgt acagtctgtt gcgacaaagc ttatatgtga
      661 cagggaagaa gaaatcgaaa actttactcc tcaggaatat tggtcaatcg aaacggtttt
      721 gaatgatccg aaaagtaaaa aggattttaa tgcaaaatat tacggtgaaa agggcaaaga
      781 agtaaagcta aacaatgccg aagaaacaaa gaaagtttcg gacgcaatag caaatgccga
      841 ttttgttata gaaaaggtta aaagaggtca gcaaaagcgt aatcctcaac cgccgtttac
      901 gacaagtact ctgcagcagg acgcgtcaag aaagtataat ttccaagcgg caaaaacaat
      961 gcagattgca caaactcttt acgaaggtat taacctcggt ggaaagatag gaacgatagg
     1021 tcttataacg tatatgagaa cagattcgct aagaattgcc gacgacg
//
LOCUS       DBMDVO010000642         1064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64029_length_1064_cov_1.104881, whole genome shotgun sequence.
ACCESSION   DBMDVO010000642 DBMDVO010000000
VERSION     DBMDVO010000642.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64029_length_1064_cov_1.104881"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(76..>1064)
                     /locus_tag="ACZPE2_08145"
     CDS             complement(76..>1064)
                     /locus_tag="ACZPE2_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1319857.1"
                     /translation="KYLLCNGPFHVSKWTEKTSVKLVKNNEYNGESSVSPSSVTFYIN
                     NDDSEVADKMNSVTYDVAFLSSSQFSSIENKKDLNSVAVQNVTYSFIFNQNDNNYRNK
                     NMRLAFCRSCDTSSLFDSQSDISKADGIVPPSCKIGGEDYRNGNAAELLSYDETLAKK
                     NFNDALLELGSSSVETTILCTEQYEPFVKQMVQKLQKTLGVKFVSTVKVVSESDLEAA
                     VNDGNYSVAFYPFKAKSSFAGEIIEGFDKNNIFGYESDVLSQKIRNIKNNSGSLEGER
                     SAVADAENYLVSEAVIMPVFYENSYFVTSKKTSGIYFYSSADNVCFINAKKS"
BASE COUNT          274 a          240 c          221 g          329 t
ORIGIN      
        1 tgtgagcgtc caagatattg ctatatagat tttttgcgat acgccgtatt tgtttttcat
       61 tttgaatacc gtctcttacg atttttttgc atttatgaaa cagacgttat ccgcagagga
      121 ataaaagtat attccgcttg tcttttttga tgttacaaaa tagctgttct cataaaacac
      181 gggcattatt accgcctcgg aaacaagata gttctccgca tcggcaacgg cggaacgctc
      241 accctcaagg ctgcccgaat tatttttgat attacggatt ttctgtgata aaacatcgga
      301 ctcatatccg aatatattgt ttttgtcaaa gccctctata atttcgcccg caaaagacga
      361 tttcgccttg aacgggtaaa acgccacgct gtaattgccg tcgtttaccg ctgcctcaag
      421 gtcggattcc gaaacgacct ttaccgtgga gacgaatttt actccgaggg ttttctgaag
      481 cttttgcacc atctgcttca cgaacggctc atactgctcg gtgcaaagta ttgtagtttc
      541 gaccgacgat gagccgagtt ccaaaagtgc gtcgttgaaa ttcttttttg caagcgtctc
      601 gtcataagac aaaagctctg cggcatttcc gtttcgataa tcctcgccgc ctatcttaca
      661 tgacggcggc actatgccgt ctgcctttga aatatcgctt tgcgagtcaa acagcgacga
      721 agtatcgcac gagcggcaaa atgcaagccg catattttta ttgcggtaat tattgtcgtt
      781 ttgattgaat ataaaggaat aggttacgtt ttgaaccgca accgaattca aatccttttt
      841 gttttctatt gacgagaact gcgatgacga gaggaaagcc acgtcatatg taaccgaatt
      901 catcttatcg gcgacttccg aatcatcgtt gtttatatag aacgtcacgg acgacggcga
      961 aaccgaggat tctccgttgt actcgttatt tttaaccagc tttactgagg tcttttccgt
     1021 ccattttgac acgtgaaacg gtccgttgca caaaagatat ttcg
//
LOCUS       DBMDVO010000643         1061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64244_length_1061_cov_1.417708, whole genome shotgun sequence.
ACCESSION   DBMDVO010000643 DBMDVO010000000
VERSION     DBMDVO010000643.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64244_length_1061_cov_1.417708"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            422..1015
                     /locus_tag="ACZPE2_08150"
     CDS             422..1015
                     /locus_tag="ACZPE2_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1319858.1"
                     /translation="MSEKAVDSRVRRTKRLLKQGLTELMAQKSIKKITVRELSELVDI
                     NRGTFYLHYKDIYDLVEQIENELFQEFESILMSYTIDDISRRPKQLLTDVCKFLDENR
                     DICSALLGDNGDINFAQKLRGFIRKKCFNDISAAYNISDVSEFEFLYSYFESGFIGVA
                     RYWLQHPETGKSAEDIADIIERIFVFGVKDYIKGEKR"
BASE COUNT          328 a          206 c          205 g          322 t
ORIGIN      
        1 gcttttcggc gtcgtctttt gctttcttta taattttgtc ggcctcggac gcagcgttct
       61 tttcgttctc cgccgcctcg ttttccgtgg ccataacctt tttcagcatt tctgccgcca
      121 tacgcttcac ctcatatcag attttcattt attatatgca ttttcataaa aagcagcttt
      181 agaagaagta taccataaaa aaactgtttt gtaatgattt ttataaaaaa tatattattt
      241 ttgtaaaaat attatcaatt ttagttccct atattatcaa catcagccta ttatacagat
      301 tttttcaaaa aagtgttgaa taatgaactt atttatatta taataacgtg tgtctattaa
      361 atcaataaac aaactatgtt tgtttgtcta aaatcagcaa aggaaagggc gtttaagcaa
      421 aatgtcagag aaagcagttg acagcagagt acgcagaacc aaaaggcttt taaagcaggg
      481 cctcaccgag cttatggctc aaaaaagcat taaaaaaata acggtacggg agctttcgga
      541 gcttgtagat ataaaccgcg gtacgtttta tctgcattac aaagacatat atgaccttgt
      601 ggagcaaata gaaaacgaac tgtttcagga atttgagtct atacttatga gctacaccat
      661 agacgatatt tcccgccgtc cgaagcagct tttgaccgac gtgtgcaaat ttctcgatga
      721 aaaccgcgat atttgctcgg ctctcctcgg agacaacggc gacataaatt tcgctcagaa
      781 gctccgcggc ttcatacgta aaaaatgctt taacgacata tccgcggcat ataatataag
      841 cgatgtatcc gaatttgaat ttctctattc gtattttgag tcaggcttta taggcgttgc
      901 gcgctactgg cttcagcatc cggagacagg caagagtgcc gaggacatag ccgatataat
      961 cgagagaata ttcgtattcg gcgtaaaaga ctatatcaag ggagaaaagc gataagatga
     1021 ccgagctttt aataaggctt tttgtgggca aaaacgcgtc c
//
LOCUS       DBMDVO010000644         1059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64418_length_1059_cov_1.155532, whole genome shotgun sequence.
ACCESSION   DBMDVO010000644 DBMDVO010000000
VERSION     DBMDVO010000644.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64418_length_1059_cov_1.155532"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="ACZPE2_08155"
     CDS             <1..959
                     /locus_tag="ACZPE2_08155"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1319859.1"
                     /translation="IAIIQVKNGTITLPQAFLIIMLAADFFLPLRLLGSFFHVAMNGM
                     AASDKLFKLLDTKEDEHGAEIPENLDGDIEIKDLSFSYDGEKTVLNDISATFKKHELI
                     SIVGESGCGKSTLASLLCGTTKGYIGSITIGSVEIKDIDEKTLMNNITAVNFNSYIFA
                     GTVRENMLIADKSAGDEKMIEALKMVNLWSFLSEQDGLDTKLNQQGSNFSGGQRQRLA
                     IARALLHNTPIYVFDEVTSNIDAESENDIMTVIHNMAKIKTVILISHRLENVVGSDKI
                     LLLDKGKIEESGTHSELMSLNKKYKLMYSTQAELEKYAKEEA"
BASE COUNT          354 a          201 c          216 g          288 t
ORIGIN      
        1 taattgcaat cattcaggta aaaaacggca caatcacttt acctcaggca tttttaatca
       61 ttatgcttgc ggctgatttc tttttaccgc tcaggcttct cggttcattt ttccatgtgg
      121 ctatgaacgg tatggcggca agcgacaaac tctttaagtt gcttgataca aaagaggacg
      181 aacacggagc ggagattcct gaaaatcttg acggggatat cgaaattaaa gacctttcct
      241 tttcttatga cggcgaaaaa accgtactta acgatatttc ggcaaccttt aaaaagcacg
      301 agcttataag catagtaggc gaatcgggtt gtggcaaaag cacgcttgct tccctgcttt
      361 gcggaacaac taaaggttac atcggcagta taacaatcgg cagtgttgaa ataaaagata
      421 ttgatgaaaa aacacttatg aacaacatca ctgccgtaaa ctttaattcc tacatttttg
      481 caggaacggt tagggaaaat atgctcatag ccgacaaaag tgccggtgac gaaaagatga
      541 ttgaagcatt gaaaatggta aacctttggt catttttaag tgagcaagac ggacttgata
      601 ctaaacttaa tcaacaaggc agcaattttt cgggcggaca aaggcaaagg cttgccattg
      661 caagagcctt gcttcacaac acaccgatat atgtttttga tgaagtaact tcaaacatag
      721 acgccgaaag cgaaaacgac attatgaccg ttattcacaa tatggctaaa atcaaaaccg
      781 ttattcttat ctcccacagg cttgaaaatg tggtaggcag tgacaaaatt cttctgcttg
      841 ataaaggcaa aattgaggaa agcggtactc attctgaact tatgtctctt aataaaaaat
      901 acaagctgat gtattccact caagcagaac ttgaaaaata tgcaaaggag gaagcgtaat
      961 gaaaagaaaa caatcaggtt tcaaaattat gatgcggttg ataaaacttg ttaagccgct
     1021 tgctttcttc atggtacttg cagttatact cggcgttac
//
LOCUS       DBMDVO010000645         1059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64430_length_1059_cov_1.046973, whole genome shotgun sequence.
ACCESSION   DBMDVO010000645 DBMDVO010000000
VERSION     DBMDVO010000645.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64430_length_1059_cov_1.046973"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(104..643)
                     /locus_tag="ACZPE2_08160"
     CDS             complement(104..643)
                     /locus_tag="ACZPE2_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1319860.1"
                     /translation="MKHLKRNVTIAAVLLLVCAAVYLNWSYNNKWGTADPAMVKAEDA
                     AMKAANEAYEAANTKSSTGYFAEARLNRQVSRDEAIKLLEDAVKSDTASQETIDSAMT
                     SISAMANYSMKESQLENKLLAKDFSDCVVYMSEDSITVAVPAPKDGLSEAAVARITET
                     VTSETSYTAAQLNVIEVPS"
     gene            complement(645..980)
                     /locus_tag="ACZPE2_08165"
     CDS             complement(645..980)
                     /locus_tag="ACZPE2_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319861.1"
                     /translation="MGDKLKAQLNKFKYPLLALVLGLIIMLLPIGESSSRSVSSQTDE
                     QRLAAVLQRCDGVGNVTALLSDTGAVIVCDGADDAAVRLSIIKAVEAFTGFSSDSIQV
                     IKTAPNIGG"
BASE COUNT          235 a          290 c          245 g          289 t
ORIGIN      
        1 tgcctccgtt ttgtctacac catgggtgca aaaattctgt catagtattg taccacaaca
       61 caagtcaaaa ataaagaaaa aagacgggaa tttcccgtct tttttacgat ggaacctcta
      121 taacattgag ctgagcggca gtatacgagg tttcagaggt tacagtctcg gttattctcg
      181 caacggcagc ttcgctcagc ccatctttag gtgcgggaac tgcaacggtt atggagtcct
      241 cgctcatata cacaacgcag tcggaaaagt ccttggcaag cagtttgttt tcaagctgcg
      301 attccttcat cgaatagttt gccatagcgg atattgacgt catggcggag tctatcgtct
      361 cctgagaagc cgtgtcggat ttcaccgcat cctcgagcag ctttattgcc tcatctcgcg
      421 atacctgacg attgagcctg gcttcggcaa agtagccggt tgagcttttt gtgtttgccg
      481 cttcatacgc ttcgtttgcc gccttcatcg ccgcgtcctc ggcctttacc atggcagggt
      541 ctgccgttcc ccacttgttg ttgtacgacc agttgagata caccgccgca catacgagca
      601 gcagtaccgc cgcaatagtc acgtttcttt taagatgctt cattttaccc tcctatattc
      661 ggcgcagttt ttattacctg aatactgtcg cttgaaaagc ccgtgaaagc ttcaacagcc
      721 ttgatgattg acaggcgtac cgcggcatca tccgcgccgt cgcacacgat aacggcaccg
      781 gtatcggaca aaagcgcagt cacattgccg acaccgtcgc acctttgcag aaccgccgca
      841 agccgctgct catccgtctg agagcttacg ctccggctcg agctttcgcc tatcggtagg
      901 agcattatta tcagtccgag cacgagcgcg agcagcggat atttaaactt attcagttga
      961 gctttgagtt tgtcacccat tttcaccgtc gctccaatcc tgattttcta cgcttatgcc
     1021 gagctgcgtc tgaatgcagt cggaaagttt agccctctg
//
LOCUS       DBMDVO010000646         1059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64431_length_1059_cov_1.021921, whole genome shotgun sequence.
ACCESSION   DBMDVO010000646 DBMDVO010000000
VERSION     DBMDVO010000646.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64431_length_1059_cov_1.021921"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /gene="mnmA"
                     /locus_tag="ACZPE2_08170"
     CDS             <1..751
                     /gene="mnmA"
                     /locus_tag="ACZPE2_08170"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1319862.1"
                     /translation="IKFSKLLKKAIELKMDLIATGHYARIEKSADRYLLKKALDESKD
                     QSYVLYSLTQDELSRTLFPLGKYRKSEVREIAEENGFINAKKHDSQDICFVPDGNYSA
                     FIEKYTGEKFPEGDFVDKSGKKLGTHKGIIRYTVGQRRGLGLALPQSMYVCEKDVKNN
                     RVVLGFNEDLFSKEVSVGDIVLSACDSLEKPEHLSAKIRYNQKEQPATVIQTDKDKLK
                     IIFDEPQRAVTKGQAAVVYDGDTVIGGGTIL"
BASE COUNT          358 a          235 c          239 g          227 t
ORIGIN      
        1 catcaaattt tccaagctct taaaaaaagc cattgagctt aaaatggacc tcatagcgac
       61 cggtcactac gcgagaatcg aaaaaagcgc agacagatac cttttaaaaa aggcgcttga
      121 cgaaagcaag gatcaaagct acgttttgta ttcgctgact caagacgagc tcagccgcac
      181 gctgtttccg ctcgggaaat accgcaaatc cgaggttcgc gaaatagccg aggaaaacgg
      241 ctttataaac gcaaaaaagc atgacagcca agatatatgc ttcgtccccg acggaaatta
      301 cagcgccttc atagaaaaat acaccggaga aaaattcccg gagggcgatt ttgttgacaa
      361 gtccggcaag aagctcggca cgcacaaggg aataatcaga tacaccgtgg gtcagcgccg
      421 cggtctcggt ctggcactgc cgcagtctat gtatgtatgc gaaaaggacg ttaaaaacaa
      481 cagggtcgtt ttaggattta acgaagatct gttttcaaaa gaggtctccg tcggagacat
      541 agtactcagt gcctgtgatt ctctcgaaaa gcccgaacac ctcagcgcga aaatacgcta
      601 caatcaaaag gaacagcccg caacggtaat tcaaaccgat aaagacaagt tgaaaatcat
      661 ttttgacgag ccgcagagag ctgttacaaa gggtcaggca gcggttgtat acgacggaga
      721 cacggttata ggcggcggaa ctattttata acctccgtac aaattgaaag cttaaatatg
      781 ataaaaataa gaaacttaaa aaaacaggat attccctccg ccgaatatat atgcttaata
      841 acggcggcaa aagaaattaa ggacacccca aaaaaagctc ttcgcaccct tttaatgtat
      901 aaccgctgtt atacgagaac ccaaaacagc agctgctttg tcgcggaaga tgaaaacggc
      961 aaagttgtgg ggtacattct ctgtgccgag agcttgccga aatatctcaa aggttttttc
     1021 ggcacggagc tgcaagagat attcaaaata agctttata
//
LOCUS       DBMDVO010000647         1059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64437_length_1059_cov_0.965553, whole genome shotgun sequence.
ACCESSION   DBMDVO010000647 DBMDVO010000000
VERSION     DBMDVO010000647.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64437_length_1059_cov_0.965553"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="ACZPE2_08175"
     CDS             <1..421
                     /locus_tag="ACZPE2_08175"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1319863.1"
                     /translation="SAAYGSLDVLTAVFKQLKDYGFHISIDDFGSGYSSLNMLKDLPA
                     DVLKIDRAFLAESNSNKRANDILGYVIRIAGSLGMHTICEGIETDDQAKLLGGLGCEM
                     AQGFYFAKPMPSDSFEEILRGNSKTINIPSNNPEEDK"
     gene            418..1035
                     /locus_tag="ACZPE2_08180"
     CDS             418..1035
                     /locus_tag="ACZPE2_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319864.1"
                     /translation="MSISNESLPIIAGIITNTARSMTTVMQYIYTVSDSDFYNINIKD
                     VFRIALMDVTETSRLENLGIRIKTPENEAMFETAEFGRVQHLIMYSLAVRLPFIARQT
                     EDFPLSDKQLKQVYELMIKNGADNFGEIIYESYEGNFKVRKQKNPLPSYSSDWFRRYV
                     YTYMPKFGEINNRNLYFLGCVEAMFPLYYSAMTAQLKKVMFLLDK"
BASE COUNT          318 a          202 c          250 g          289 t
ORIGIN      
        1 gagtgcggct tatgggtcgc tcgatgtgct tacggctgtg ttcaagcaac tcaaggatta
       61 cggattccat atttcaatcg atgatttcgg ctcgggatat tcttcgctca atatgctgaa
      121 agatcttcct gctgatgtgc tgaagataga cagggcgttc cttgcggaat caaacagcaa
      181 caagagagcc aacgatatac tgggttatgt tatcagaata gcaggctcac ttggtatgca
      241 tacaatatgc gagggtattg aaaccgatga tcaggcaaag ctgctcggcg gtcttggctg
      301 tgagatggca caaggtttct actttgcaaa acctatgccg agtgattcct ttgaagaaat
      361 tcttagaggc aacagtaaga ctatcaatat tccgagtaat aatcccgagg aggataaatg
      421 agcatttcaa atgagtcact gccgataata gcgggaatta tcactaatac ggcaaggtca
      481 atgactacgg taatgcagta tatctatact gtgtccgaca gtgattttta caatatcaat
      541 ataaaagatg tctttcgtat cgcactgatg gacgttaccg aaacatcacg gcttgaaaat
      601 ctcggaataa ggataaaaac acccgaaaac gaggcaatgt tcgaaacggc tgagttcggc
      661 agggtacagc atcttattat gtacagcctt gcggtaaggc tgccgtttat cgcacgtcag
      721 acagaagatt ttccgctgtc ggataaacag cttaaacagg tgtatgagct gatgataaag
      781 aacggtgcgg ataatttcgg agagattatc tatgaaagct atgagggcaa ttttaaagtc
      841 agaaagcaga aaaatccttt gccgtcatat tcatcggact ggttcagacg ttatgtttac
      901 acatatatgc cgaagttcgg tgagataaat aaccgtaacc tttatttcct cggctgtgtt
      961 gaggctatgt ttccgctgta ttattcggcg atgacggcac agcttaaaaa ggttatgttc
     1021 ctgcttgata agtgaattaa aatcaaatcc cacaaacgt
//
LOCUS       DBMDVO010000648         1059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64442_length_1059_cov_0.912317, whole genome shotgun sequence.
ACCESSION   DBMDVO010000648 DBMDVO010000000
VERSION     DBMDVO010000648.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64442_length_1059_cov_0.912317"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="ACZPE2_08185"
     CDS             complement(<1..239)
                     /locus_tag="ACZPE2_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319865.1"
                     /translation="MDGQGYTVNLKDTYTIVGKSNITLRNVIFDMKDYVASNNSMFVF
                     WNGITFTLDNGAVIRNYTATGDTVFGSGCVFMLNGG"
     gene            complement(258..>1059)
                     /locus_tag="ACZPE2_08190"
     CDS             complement(258..>1059)
                     /locus_tag="ACZPE2_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319866.1"
                     /translation="TIILTKATEAKRETAEDNYFNNRVFTVEGNGSTLSIEKSFPMWG
                     DKGHITFKNVTVEMDNFCLGNSSKMTFDEGSVLTTPAAGVTGKVISVQGGGSFTPTIT
                     MKNGSAIKNAKSTVGVISLDVGVATLNMEDGVTVTGVEAQRLVDCYQQSILNIAGATI
                     KDNTLKDGVVYAVAKTINVTNPSALTVENNGTNRIIAAATTNTLTVNVTGDWDSALTI
                     DNKNYYVKSSIVAKTDAGLYESLNDAITATNNKDTVIYLLKDCDLNKL"
BASE COUNT          271 a          244 c          194 g          350 t
ORIGIN      
        1 ccgccgttaa gcatgaatac gcaaccactt ccgaaaacag tatctcctgt tgctgtgtaa
       61 tttctgatta ctgctccgtt gtcaagtgtg aacgtaattc cgttccagaa aacaaacatc
      121 gaattattgc ttgcaacgta atctttcatg tcaaatataa catttctaag agtaatattc
      181 gacttcccga caatcgtgta tgtatcttta aggtttaccg tgtaaccctg accgtccaaa
      241 atatagcttc tgtcgattca gagcttgtta aggtcacaat ccttaagaag ataaataacc
      301 gtatctttgt tatttgtagc agttatagca tcattaagcg attcatagag ccccgcatcg
      361 gtctttgcaa cgatgctgct cttaacataa taatttttat tatcgatcgt taatgcgctg
      421 tcccagtctc cggtaacatt tacggttaaa gtatttgtgg tcgctgcagc aattattctg
      481 ttggtgccgt tattttcaac agttaatgcc gaaggattgg ttacattaat tgtcttcgca
      541 acagcatata caactccgtc cttcaatgta ttatccttta ttgttgctcc ggcaatattc
      601 aaaattgatt gctgatagca gtctacaagg cgctgtgctt caacacctgt aactgtaaca
      661 ccgtcctcca tgttaagtgt tgcgacacca acatcgagac ttattacgcc aactgtgctt
      721 ttcgcgtttt taattgcact tccgttcttc atcgttattg tgggagtaaa actacctccg
      781 ccctgaaccg atattacctt gccggtaaca cctgccgcag gtgtggtgag aaccgagccc
      841 tcatcaaacg tcatcttaga gctgttaccg agacagaaat tatccatctc aacagtgaca
      901 tttttaaacg ttatatgccc tttgtcgccc cacatcggga agcttttttc aattgaaagc
      961 gtgcttccgt ttccctcaac agtgaagact ctgttgttga aatagttatc ctcagccgtt
     1021 tctctttttg cctctgttgc ttttgtgagg ataattgtg
//
LOCUS       DBMDVO010000649         1057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64596_length_1057_cov_1.100418, whole genome shotgun sequence.
ACCESSION   DBMDVO010000649 DBMDVO010000000
VERSION     DBMDVO010000649.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64596_length_1057_cov_1.100418"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            563..>1057
                     /locus_tag="ACZPE2_08195"
     CDS             563..>1057
                     /locus_tag="ACZPE2_08195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1319867.1"
                     /translation="MISCFKGRFRLTSPRGDRVLNGIKGYHHGIDLVGLDDTTVYSIV
                     DGKVRTGADNSAGNYVCVTIEDGRRVYYFHLKSFKVKTGDTVKKGQAVGIMGNTGHSF
                     GAHTHLELRVKGTAYKSLDISEFTGIPNEVGVYEYKEDGVKQNKYSYDDTVDAMIQDG
                     VTDVK"
BASE COUNT          300 a          214 c          283 g          260 t
ORIGIN      
        1 gcctacatca tcggcgaagg gctggtggat gcggcgagaa tcaaggcaca gacaggcgcg
       61 gcggatacag aagccgctca cgagaccggc aaataagcat aaaaaagccg ctcaaggcga
      121 acatgagcgg tattgacata acgggaaaac tgatgtataa tagaatctgc cgggagcaat
      181 tccggcaggg cgagatctca ttctgaacct gaaaaagatg tttctgtttc gagttctttg
      241 gctcttcctt tcttcaaaag aaaggtagac ggcggttgtg ccattcccga aagggggtgg
      301 ttgttatgca gaaatacata agattcatta tctttgctat tctcatagca atagctttta
      361 cattaaaagt aaaataaccg ccccaagcat cactttgtag cggttatttt taactgatat
      421 atcgtggcgc aaccgtttga ggtcttgccc ttacattact attatatgct ttttcctcaa
      481 aaagtcaata cctttatccg aaattttcgc cgtgagtttt tacttgcggc gatatttttt
      541 gaaaagatag gaggaaaaaa gaatgattag ttgcttcaaa ggacgtttcc gtttaaccag
      601 tccgcgtggc gaccgagttc taaacggtat taagggctac catcacggca ttgaccttgt
      661 cggtttagac gacacgacgg tgtacagcat tgtcgatgga aaagtccgca caggggcaga
      721 caacagtgcc gggaactatg tgtgtgtgac catcgaggac ggcaggaggg tatattattt
      781 tcatttgaaa agcttcaagg tcaagaccgg cgataccgtc aaaaagggac aggcggttgg
      841 aatcatgggc aacaccgggc acagctttgg ggcgcatacg catttggagc tgcgtgtcaa
      901 aggcacggcc tacaagagtc tcgatatttc cgagtttacc gggataccga atgaggtcgg
      961 tgtgtatgag tacaaggagg acggcgtgaa gcagaacaaa tacagctatg acgataccgt
     1021 ggatgccatg atacaggacg gtgtgacgga tgtgaag
//
LOCUS       DBMDVO010000650         1056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64694_length_1056_cov_1.245026, whole genome shotgun sequence.
ACCESSION   DBMDVO010000650 DBMDVO010000000
VERSION     DBMDVO010000650.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64694_length_1056_cov_1.245026"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /gene="grpE"
                     /locus_tag="ACZPE2_08200"
     CDS             <1..568
                     /gene="grpE"
                     /locus_tag="ACZPE2_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1319868.1"
                     /translation="IMKGLKTMAKKKETEPKGTDKKNEKINGEKTDNVEIEVEKEVSE
                     AESLASQLKDVNDRLLRTLAEYDNFRKRSQKEKEAAYSDSKALVLAELLPVMDNFERA
                     AENKNASPEDYMKGIDMIYNQFSEVFKKLGVESFGEKGDSFDPNLHNAVMHEDDENEK
                     ENVITDVFSKGYKLGDKILRPAMVKVAN"
     gene            599..>1056
                     /locus_tag="ACZPE2_08205"
     CDS             599..>1056
                     /locus_tag="ACZPE2_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1319869.1"
                     /translation="MAKVIGIDLGTTNSCVAVIEGGEPVVIANAEGARTTPSVVAFSK
                     TGERMVGQVAKRQAITNPERTVSSIKRHMGSDYKVTIDDKSYTPQEISAMILQKLKGD
                     AESYLGGKVTEAVITVPAYFTDSQRQATKDAGKIAGLDVKRIINEPTAAA"
BASE COUNT          345 a          219 c          265 g          227 t
ORIGIN      
        1 cataatgaaa gggctgaaaa caatggcaaa gaaaaaagaa acagagccta aaggcactga
       61 taaaaagaac gaaaaaatca acggcgaaaa gaccgataac gtagaaatcg aggtcgaaaa
      121 agaggtgtcc gaggccgaga gcttagcttc tcagcttaaa gacgttaacg acagactttt
      181 acgtacactt gccgagtatg ataatttccg taagcgctct caaaaagaaa aagaggcggc
      241 gtacagcgat tcaaaggcac ttgttttggc ggagctttta cccgtgatgg ataattttga
      301 acgcgccgca gaaaacaaaa acgcatcgcc cgaggattat atgaagggga ttgatatgat
      361 ttacaatcag ttctccgagg tgtttaaaaa gctcggcgtt gagtcgttcg gcgaaaaggg
      421 cgattccttt gaccccaatc tccacaatgc ggttatgcac gaggacgacg aaaacgaaaa
      481 ggaaaacgtc attaccgacg tattttcaaa aggctataag cttggcgaca aaatattgcg
      541 tcccgcaatg gtcaaggttg ccaattaaat aataaataat gttttggagg aataaattat
      601 ggctaaggta ataggtattg acttaggtac tacaaattca tgcgtagcgg ttatcgaggg
      661 cggcgagcct gttgttatcg caaacgccga gggtgcaaga accactccgt cggttgtcgc
      721 attctcaaag accggcgaga gaatggtcgg ccaggttgca aagcgtcagg caatcacaaa
      781 tcccgagaga accgtttctt cgataaagag acatatgggc tcggattata aggttaccat
      841 agacgataaa agctacactc cgcaggaaat ttccgcaatg attcttcaga agctcaaggg
      901 cgacgcagag agctatctcg gcggcaaggt tacagaggct gtcattacgg ttcccgcata
      961 tttcaccgat tcacagcgtc aggctactaa ggacgcaggc aaaatcgcag gccttgatgt
     1021 taagagaata atcaacgagc cgacagccgc agcttt
//
LOCUS       DBMDVO010000651         1054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_64889_length_1054_cov_0.799580, whole genome shotgun sequence.
ACCESSION   DBMDVO010000651 DBMDVO010000000
VERSION     DBMDVO010000651.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64889_length_1054_cov_0.799580"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="ACZPE2_08210"
     CDS             complement(<1..932)
                     /locus_tag="ACZPE2_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HIR1319870.1"
                     /translation="MKKRVGIIGGGASGLVAAIGAARNGAEVTVIEHMDRVGKKILST
                     GNGRCNILNFDAVAENYFEACEFVRPAVEKYTPKKTEAIFRDMGLLLMSEQEGRAYPI
                     SQTAASVVCVLSSAAERAGVKVFTDTPVKSIKKSQGGFVINNDKSFNFAVIACGGAAA
                     PKQGTDGSSFELFKALGIRVTELSPALTGMCVKDMPFSLKGLRQYCKCTVFSNEKEYV
                     ESGEVQFNDYGLSGIPIMQLSRFIGKGKATVTLDCLPSLTKNEITAFLQSHKFREPSG
                     DVLCGILPRALANFVLRECNVKKDEPFASLSSDKA"
BASE COUNT          270 a          283 c          203 g          298 t
ORIGIN      
        1 gctttatcac ttgacaggct tgcgaacggc tcgtcctttt ttacgttaca ttcgcgaagg
       61 acaaaatttg cgagtgctct cggtagaatt ccgcacagca catcacccga cggttcacgg
      121 aacttgtggg attgcaaaaa agctgtaatc tcgttttttg taagcgacgg caaacaatcg
      181 agcgtaaccg tagctttacc tttgcctata aagcgcgaga gctgcattat aggtataccc
      241 gaaagtccgt aatcgttaaa ttggacctca ccggactcaa catattcttt ttcgtttgaa
      301 aaaacagtgc atttgcagta ttgcctcagt cctttaagtg aaaagggcat atcctttaca
      361 cacattccgg taagtgccgg agagagctct gtcacacgta ttccgagggc tttaaacaac
      421 tcaaacgagc tgccgtccgt cccctgctta ggtgctgcgg cacctccaca ggcaataacc
      481 gcaaaattga agctcttatc attatttatt acaaatccgc cttgtgattt cttaatgctt
      541 ttgacaggtg tatctgtaaa cacctttaca cctgctcttt cggcagcaga tgaaagcaca
      601 cacaccaccg aggcggcagt ctgtgaaatc gggtatgctc tgccctcctg ctcactcatc
      661 aaaagcaaac ccatatcgcg aaaaatcgct tctgttttct tcggagtata tttttctacc
      721 gccgggcgca caaattcgca tgcctcaaag taattctcgg caactgcatc aaaattcaag
      781 atattacacc tgccgttgcc tgtagaaagg atcttttttc ccacgcggtc catatgttct
      841 ataacagtca cttccgcccc gtttcgggct gctcctatag cagccaccag cccggacgcg
      901 ccaccgccaa taattcctac tcttttcttc atatttttta actcctgttt taacttgtat
      961 acgactccag ataatggtat acgtccatca tatctgaaaa ccagatgcct tccatcagtc
     1021 gggctctttc actttccgga aaatcagtta ccgg
//
LOCUS       DBMDVO010000652         1050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65190_length_1050_cov_1.390938, whole genome shotgun sequence.
ACCESSION   DBMDVO010000652 DBMDVO010000000
VERSION     DBMDVO010000652.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65190_length_1050_cov_1.390938"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            163..>1050
                     /locus_tag="ACZPE2_08215"
     CDS             163..>1050
                     /locus_tag="ACZPE2_08215"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR1319871.1"
                     /translation="MLKKEGEVRIPSGCAIAAITDRTGERFDGTDIIDCIALMHDRSN
                     GLGGGFAAYGIYPDYKDLFAFHIFYDDNDARIKTEEFLFRHFDIENFEQIPTRKVKSI
                     HHAPLIWRYFGAVPASKIKKNDFDEDEYTARCVIKINNEIDGAFVFSSGKNMGAFKAV
                     GYPEDVGEYYMLENYKAHTWTAHGRFPTNTPGWWGGAHPFTLLDWSIVHNGEISSYDA
                     NRRYVEQFGYRCNLQTDTEVITYLFDLLVRRQGLRLDTAAQILAAPEWDVIDRMPEEK
                     KKVYTVLRTAYSSALINGPF"
BASE COUNT          304 a          224 c          251 g          271 t
ORIGIN      
        1 aatctgcttt ttctgtgtaa ttgttgaata acaagggcta atccacttga aaaaaattaa
       61 aatccgatat aaaatatttc ccatatttat atcaaagtga aagttgctaa cgcaaaactt
      121 tcattttgca atatgcaata aattaaaacc ggagatgttt ttatgctcaa aaaagaaggc
      181 gaagtgcgta taccgtccgg ctgtgccatt gcggcgataa ccgacagaac gggcgaacgc
      241 tttgacggca cggatataat agactgcatt gcgctgatgc atgaccgctc aaacggtctc
      301 ggcggcggct ttgcggctta cgggatttat cccgactata aggatttgtt tgctttccat
      361 attttctatg atgacaatga tgcacgaatt aaaaccgagg agtttttatt cagacacttt
      421 gacatagaga attttgagca gatacctacg cgtaaggtaa agtcaataca ccacgcgccg
      481 cttatatggc gttatttcgg agccgtaccg gccagtaaaa tcaaaaagaa cgactttgac
      541 gaggacgagt acacggctcg ctgcgttatc aaaataaata acgaaataga cggtgcgttc
      601 gttttttcaa gcggaaaaaa tatgggcgcg tttaaagccg tcggctaccc cgaggatgtc
      661 ggcgaatatt atatgcttga aaactacaag gctcacacgt ggaccgctca cggcagattt
      721 cccacgaaca ctcccggctg gtggggcggc gcgcacccgt ttacgctgct tgactggtca
      781 atagtccata acggcgagat ttcaagctat gacgcaaatc gacgttacgt tgagcaattc
      841 ggttatcgct gtaatttgca gaccgatacc gaggttataa catatctttt tgatttgctt
      901 gtaaggcgtc aaggcttaag acttgatacg gcggcgcaga tattggcggc acccgaatgg
      961 gatgttatag accgtatgcc cgaggaaaag aagaaggtat acaccgttct caggactgcg
     1021 tattcaagcg cgctgataaa cgggccgttc
//
LOCUS       DBMDVO010000653         1047 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65414_length_1047_cov_1.217759, whole genome shotgun sequence.
ACCESSION   DBMDVO010000653 DBMDVO010000000
VERSION     DBMDVO010000653.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65414_length_1047_cov_1.217759"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="ACZPE2_08220"
     CDS             complement(<1..501)
                     /locus_tag="ACZPE2_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1319872.1"
                     /translation="MAKRRIGILTSGGDCPGLNATIRGAAKACYSMFGEDNVEIVGIS
                     NGFHGLIHNNCRIMKPEDFSGILTQGGTILGTKRTPYKMMQVIEADNIDKVAEMKATY
                     KQQKLDCILTLGGNGTHKTANLLSEEGLNIIGLPKTIDNDIWGTDVTFGFHTAVDIAT
                     EVVDRIH"
     gene            complement(624..>1047)
                     /locus_tag="ACZPE2_08225"
     CDS             complement(624..>1047)
                     /locus_tag="ACZPE2_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR1319873.1"
                     /translation="NDIASFISSLSGEKLCRILSSTESCSVETKLPKFSYEYSDTLNN
                     ALSQLGMPTAFDENNADFSGIGKSTVGNISIGKVIHKTKIEVNEKGTKAGAAALVEMV
                     DNACAINEKQVILDRPFMYMILDNETMLPLFAGIYTGV"
BASE COUNT          242 a          239 c          229 g          337 t
ORIGIN      
        1 gtgtattctg tcaacgacct cagtcgcaat atctactgcg gtatggaagc cgaatgtaac
       61 atctgttccc catatatcgt tatctattgt cttgggaagt ccgattatat tcagaccctc
      121 ttcggaaaga agatttgcgg tcttatgagt tccgttgccg ccgagcgtca gtatgcagtc
      181 gagcttctgc tgtttatagg tggctttcat ttcagcgacc ttgtctatgt tgtcggcttc
      241 gattacctgc atcatcttat acggtgtacg ctttgtgccg agtatcgttc cgccctgagt
      301 gagtatgccg gaaaaatcct ccggcttcat tatacggcag ttattatgga tcagaccgtg
      361 gaaaccgttg gatatgccga ctatctcaac gttgtcctcg ccgaacatgg agtaacaagc
      421 ttttgccgca cctcttatag tagcgttaag tccggggcag tcgcctccgc tcgtcagtat
      481 acctattctg cgctttgcca tttgtacatc cagcctttcc tgaaagattt gttgttttct
      541 gctaattaaa ttatagcata catactccaa aagtcaagaa aatattatag ctcggattat
      601 gcagattata caatttctgt gcgttatacg cctgtatata tgcctgcgaa cagtggcagc
      661 atcgtttcgt tgtcgagtat catatacata aacgggcggt ccaggattac ctgcttttcg
      721 ttaatagcac acgcattgtc aaccatttct acaagagcgg cggcacctgc cttagtacct
      781 ttctcgttta cctcgatttt agtcttgtga atgactttgc cgatggatat attgcctaca
      841 gttgatttgc cgatacccga aaaatccgca ttgttttcgt caaatgctgt cggcatacca
      901 agctgtgaca aagcgttgtt cagtgtgtct gaatattcat agctgaattt cgggagcttt
      961 gtttctacgc tgcagctttc ggtactgctt aatatcctgc agagcttttc tccggataag
     1021 gaggatatga aggaggctat atcatta
//
LOCUS       DBMDVO010000654         1047 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65433_length_1047_cov_0.906977, whole genome shotgun sequence.
ACCESSION   DBMDVO010000654 DBMDVO010000000
VERSION     DBMDVO010000654.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65433_length_1047_cov_0.906977"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1047)
                     /locus_tag="ACZPE2_08230"
     CDS             complement(<1..>1047)
                     /locus_tag="ACZPE2_08230"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017682.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1319874.1"
                     /translation="KDIQDRYAKLYDCRNSYLLINGTSGGLIAAILASVPAGKKLLMA
                     KNCHKSIFNALTLGNIEPVYLHPEIDEEYGITGAINPEEVKRLLAETPEAEAVILPSP
                     NYYGICSDIEAIAEVVHNAGKVLIVDQAHGAHLHFFHKYGAGDDMPESAEMSGADIVV
                     GSTHKTLASMTQSAVLNLCSDRVDRYLLEDKLQAVQSTSPSYVLMASLDINAEILEKH
                     GQQLMNKWRENLDRFYIEAANIKGLNIMKTDMMDDTKINLDMGAYGITGGQLEKLLME
                     KNIYTELNTGDILMCMTGIGNTRSDLEKLLKALEEIAAEYGTAEKSKTQRNVVIPKGG
                     RMHPVPKYKEFVKL"
BASE COUNT          222 a          257 c          224 g          344 t
ORIGIN      
        1 tccagtttca caaattcctt gtatttaggt accgggtgca ttctgccgcc ctttggtatc
       61 actacatttc tctgtgtttt tgatttttct gcagttccgt actccgctgc aatctcctcc
      121 agagctttaa gcagtttttc aaggtcgctt cgtgtattgc ctatgcctgt catacacata
      181 agtatatccc ctgtattgag ctctgtgtat atgttttttt ccataagcag tttttcaagc
      241 tgtccgcctg tgatgccgta agcgcccata tcgagattga ttttggtatc atccatcata
      301 tcggttttca taatgttaag cccctttatg tttgctgcct ctatgtaaaa ccggtcgagg
      361 ttttcacgcc acttattcat aagctgctgc ccgtgttttt cgagaatttc ggcgtttatg
      421 tcaagcgacg ccatcagcac atatgaaggg ctggtactct gcacggcctg cagcttatcc
      481 tcaagcagat atctgtccac cctgtcactg cacaggttta gcactgccga ctgggtcatt
      541 gaagcaagag ttttgtgagt gcttccgaca actatatcgg ctccgctcat ttcggctgat
      601 tccggcatat catcacctgc tccgtatttg tggaagaagt gaagatgcgc gccgtgagcc
      661 tgatctacta tgaggacctt gcccgcattg tgaacaacct ctgcaatagc ctctatatcg
      721 ctgcatatcc cgtaatagtt cggacttggc agtatcacag cttctgcttc gggggtttca
      781 gcgagcaacc gttttacttc ttcgggattt atcgctcctg ttatgccgta ttcctcatcg
      841 atttccggat gaagatagac aggttcaata ttaccgagcg tcagcgcatt aaatattgat
      901 ttatggcagt tttttgccat aaggagtttt ttgccggccg gtacagatgc aagtattgca
      961 gctataaggc cgccgctggt gccgttaatc agaagatatg aatttctgca atcatacagc
     1021 ttcgcatatc tgtcctgtat atccttt
//
LOCUS       DBMDVO010000655         1046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65502_length_1046_cov_1.017989, whole genome shotgun sequence.
ACCESSION   DBMDVO010000655 DBMDVO010000000
VERSION     DBMDVO010000655.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65502_length_1046_cov_1.017989"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="ACZPE2_08235"
     CDS             complement(<1..541)
                     /locus_tag="ACZPE2_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlgB N-terminal domain-containing protein"
                     /protein_id="HIR1319875.1"
                     /translation="MAINVPKEYEVPLYLFHQGKNAEAYKLFGSHFAEKDGVSGVVFR
                     VWAPKAADVSVVGDFNHWNREESYMKKISDGGIWELFIPGLKKFDNYKYSIKTERGQI
                     KLKADPYGYHMETRPNTASKVYDISGYNWKDSKWMDEKKTKDVYRSPMNIYEVHLNSW
                     FTKTDEEELFSYMQLAEKLV"
     gene            complement(670..>1046)
                     /locus_tag="ACZPE2_08240"
     CDS             complement(670..>1046)
                     /locus_tag="ACZPE2_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1319876.1"
                     /translation="EWDFSRYTPDYVIIALGQNDANPDPKRIYEKEYREKWKTVYKNM
                     VLDLNEKYGGKARFIVITTILMHEPTWDDALDEIVNELAMDNVKRFKFRRNGAATPGH
                     PRIPEQTEMADELYGFITGWEN"
BASE COUNT          253 a          242 c          204 g          347 t
ORIGIN      
        1 gaacgagctt ttccgcaagc tgcatatagc tgaacagctc ctcctcatcg gtctttgtaa
       61 accaggagtt gaggtgtacc tcgtatatat tcataggaga acggtatacg tccttcgtct
      121 ttttctcatc catccacttt gaatctttcc agttgtagcc ggaaatatca tacacctttg
      181 aagcggtatt aggacgtgtt tccatatgat agccgtaggg atcggccttc agctttatct
      241 gtcctctttc ggtctttatg ctgtatttgt aattatcaaa cttcttaagt ccggggataa
      301 aaagctccca gataccgccg tcgctgattt ttttcatata gctttcttca cggttccagt
      361 gattgaaatc gcctacgacg ctgacatcag ccgcctttgg tgcccatact ctgaaaacaa
      421 caccgctcac gccgtctttt tcggcaaaat gagagccgaa cagcttatat gcttcggcat
      481 tctttccctg atggaaaaga taaaggggaa cttcatattc cttaggcaca ttaatagcca
      541 taacagatac tcctaacatt ttattttact tttttaataa tagcacaatt cccaaaagtt
      601 ttcaatagat ttgtgccgat tttatgaatt gtgccttgtt tcaacctgtc tttttgtgca
      661 gattgacaat cagttttccc atccggttat aaagccgtac agctcgtcag ccatctctgt
      721 ctgctcgggg atacggggat gtccgggcgt tgccgcaccg tttcttctga atttaaaacg
      781 ttttacatta tccattgcaa gttcgttcac tatttcgtca agagcatcgt cccatgtagg
      841 ctcgtgcata agtatagtgg ttataacgat aaatcttgcc ttgccgccgt atttttcgtt
      901 aaggtcaagc accatatttt tatatacggt cttccacttt tcacggtatt ctttttcgta
      961 aatacgctta gggtcggggt ttgcgtcatt ctgacccaat gcgattataa cataatcggg
     1021 agtgtagcgt gaaaaatccc attcgg
//
LOCUS       DBMDVO010000656         1046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65506_length_1046_cov_0.977778, whole genome shotgun sequence.
ACCESSION   DBMDVO010000656 DBMDVO010000000
VERSION     DBMDVO010000656.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65506_length_1046_cov_0.977778"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="ACZPE2_08245"
     CDS             complement(<1..202)
                     /locus_tag="ACZPE2_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319877.1"
                     /translation="MCLTVNWHRGGHKLKKKRHELILRLITENAVTTQDELLELLKKN
                     GYNVTQATVSRDIKELKLIKTPV"
     gene            complement(165..>1046)
                     /locus_tag="ACZPE2_08250"
     CDS             complement(165..>1046)
                     /locus_tag="ACZPE2_08250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1319878.1"
                     /translation="TVKQVTDLKIALLPNLTRSHALSVTDDVCKYLEKYNAEYYFETE
                     TAEKIGHKIKAVGLPEAELWSLCDVVIAIGGDGSLIHAARKAVKYKKPILGVNAGNLA
                     FMAGIEKNELELLKELIEGNYTIDKRMMLDVTVKNGSCEKTLDCCLNDVVIARGEQIK
                     LVKLNVECDGQQINDYYADGIIISTPTGSTAYSLSAGGPVVDPKIESILLTPICTHSL
                     FARSLIFGNDSVLSVKIPENTSDKIRISCDGDDSVELRPGDCVRVGKSKYYADFIRIK
                     NESFIDVLNSKLAQRRA"
BASE COUNT          251 a          243 c          219 g          333 t
ORIGIN      
        1 ttacaggtgt ttttatgagc tttaattcct ttatatcacg cgaaacggta gcctgtgtta
       61 cattgtatcc gttcttttta agaagctcca agagctcgtc ctgcgtagta acggcgtttt
      121 cggttataag ccttaaaata agctcatgtc ttttcttttt caacttatgc cctcctctgt
      181 gccaatttac tgttaagcac atcaataaac gattcgtttt taatcctgat gaagtccgcg
      241 tagtattttg acttgcctac tcttacgcag tctcccggtc tcagctctac ggaatcgtcg
      301 ccgtcacatg aaattctgat tttatcggac gtgttttcgg gaattttcac cgagagcacg
      361 gagtcgttgc cgaagataag cgaccttgca aaaagtgaat gtgtgcagat aggcgttaaa
      421 agtatactct cgattttcgg gtctacaaca gggccgcctg ccgacagcga atacgccgtg
      481 gagcctgtgg gtgtggaaat tatgataccg tcggcgtaat aatcgttgat ttgttgcccg
      541 tcacactcaa cattaagctt aacgagcttt atctgctcgc ctcttgcgat tactacgtcg
      601 ttaaggcagc aatcgagcgt tttttcacat gagccgtttt taacggtgac gtcgagcatc
      661 attcgtttat ctattgtata attgccctct ataagctcct ttaaaagctc gagctcgttt
      721 ttttctatac ccgccataaa ggcgagattg cctgcattga cgccgagtat cggcttttta
      781 tatttaaccg ctttacgtgc cgcgtggata agcgagccgt caccgcctat ggcgataaca
      841 acgtcgcaaa gtgaccaaag ctctgcttcg ggcaaaccga cggctttaat tttatgacct
      901 attttttccg cggtttccgt ttcaaaatag tattcggcat tgtatttttc aaggtattta
      961 catacatcgt ccgtaaccga aagcgcatga gaacgcgtta aattcggcaa caatgcaatt
     1021 ttcaaatcag tcacctgctt tactgt
//
LOCUS       DBMDVO010000657         1042 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65829_length_1042_cov_1.018066, whole genome shotgun sequence.
ACCESSION   DBMDVO010000657 DBMDVO010000000
VERSION     DBMDVO010000657.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65829_length_1042_cov_1.018066"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="ACZPE2_08255"
     CDS             complement(<1..880)
                     /locus_tag="ACZPE2_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR1319879.1"
                     /translation="MKKIISVILALITAASLASFGVLASDSAEGILGDGNRDGKINVK
                     DIVIAIRAAIGQTMDNIDLDALDINRDGNVDVKDIIILIRHSTGCKQSYLIGYPMSSV
                     PSAKPAIKVVSGVEFITYTSSITYKGQKDEYAYTAQNDGLVRIDISELKSSVHVDLYV
                     FDRLGEDVTRRLNCSNNSGVSIQNAKAGDTYRIQVRYNTEFGDYVLTIGQPKPVIDVT
                     SFKEVRDSIQYNEQCNIYMFTPSVDGLYRFDLNNMQSDFHANIYLYDRLGYTVSSQLY
                     CSNNNGITGTDLKAGEE"
BASE COUNT          238 a          227 c          279 g          298 t
ORIGIN      
        1 actcttctcc cgccttaaga tcggttccgg ttattccgtt attatttgag cagtacagtt
       61 gtgatgatac tgtatatccg agacggtcat acagataaat atttgcatga aagtcgctct
      121 gcatattatt caggtcgaaa cggtacagac cgtcaacgga gggtgtgaac atgtaaatat
      181 tgcattgttc attgtactgt atgctgtcgc gtacttcctt gaaggaggtt acgtcgatta
      241 cgggcttggg ctgaccgatc gtgagaacgt agtctccgaa ttcagtgttg tatctgacct
      301 gaatccggta agtatcaccg gcttttgcat tctgaatgct gacaccggag ttgttcgaac
      361 agttaagccg acgagtcaca tcctcaccga gacggtcgaa tacatacaaa tcaacatgca
      421 cgctgctttt cagctccgat atgtcgatcc gcacaagtcc gtcgttctgt gcggtgtagg
      481 cgtattcatc cttttgtccc ttgtaggtta tgctgctcgt ataggtgata aactccacac
      541 ctgagacgac cttaattgcc ggctttgcgg agggaaccga agacatcggg taaccgatga
      601 ggtagctttg cttgcatccg gtcgagtggc ggatgaggat gatgatatcc tttacatcga
      661 cattaccgtc gcggtttatg tcgagcgcgt cgagatcgat gttgtccatt gtctgaccga
      721 tcgcggcgcg gattgcgatg acgatatcct ttacgttgat cttaccgtcg cggtttccgt
      781 cgccgagaat tccctctgcg gaatcgcttg cgaggactcc gaacgatgcg agggaagcgg
      841 cggtgatcag ggcaagaatt acggaaatga ttttcttcat ttgaaaatgc tttgcctttc
      901 tttaattatg tgtcgggaag ttcggaagaa gtaaactttc cctgaaaaat gcacgcggct
      961 gatttatgcg gtgagggagg atatgggctg tcctaccgga gcgttctggg tgtagttgtt
     1021 tgcatagcgg acaagaatga tg
//
LOCUS       DBMDVO010000658         1040 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_65972_length_1040_cov_1.043663, whole genome shotgun sequence.
ACCESSION   DBMDVO010000658 DBMDVO010000000
VERSION     DBMDVO010000658.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65972_length_1040_cov_1.043663"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="ACZPE2_08260"
     CDS             complement(<1..998)
                     /locus_tag="ACZPE2_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR1319880.1"
                     /translation="MEQPIECDITLPEYLPDIQRVLKCSLTAHISSVQKTGDRVTADG
                     TGRLSVLYMSDTGKLQCLEQGVPFSRFFENRAVEECECCVRAKTEYVNCRAVSGRRLD
                     IHGSISITFSCYSCERKKLICACSGGGVETKSEKNEVCNMLGCSEKGFTVSEIYEIGD
                     AKPAISQIVRSYASAVIEDTKAVSGKILIKGELTVKTLYIAETNDNELQTTEHTMPIN
                     QIVEVEKADDESINIIKLVISDLAVSAKTDAAGALRLLDAGAEIRAYTETYKSEEMSM
                     VTDAYSVNYEIESKLTPTEIRRLDDSFTDTHLCRGRLDLSGTDTERVLDMSVAGIE"
BASE COUNT          222 a          267 c          224 g          327 t
ORIGIN      
        1 tactctatgc cggcgactga catatctaaa actctctcgg tatcggtacc cgataaatcg
       61 agcctgcccc tgcaaagatg agtgtcggta aagctgtcgt caagccgccg tatctccgtc
      121 ggtgtcagct ttgattcaat ttcgtaattt accgaatatg cgtccgtcac catgctcatt
      181 tcttcgcttt tgtatgtttc ggtgtatgcg cgtatttcgg ctcctgcgtc caaaaggcga
      241 agtgctcctg cggcgtctgt cttggcagat acggctaaat ccgaaatcac aagctttatt
      301 atattgatgc tttcgtcatc tgctttttcg acttcaacta tttgatttat cggcatggtg
      361 tgctcggttg tttgcagctc gttgtcattt gtctccgcaa tatagagagt ttttacggta
      421 agctcgcctt taataagaat ttttccagat accgcctttg tatcctctat tacggcactt
      481 gcgtaggaac gcactatctg cgatatcgcc ggctttgcat ctccgatttc gtatatttcg
      541 ctgacggtga agcccttttc cgaacagccg agcatattgc acacttcatt cttttcgctt
      601 ttggtttcga ccccgccgcc gctgcatgcg caaataagtt ttttacgctc gcacgaatag
      661 catgaaaagg ttatgcttat actgccgtga atatcaagtc gcctgccgga aacggcacgg
      721 cagttgacat attccgtttt tgccctgacg caacattcgc attcctcaac cgctctgttt
      781 tcaaaaaaac gagaaaaggg aacgccctgc tcaaggcatt gcagctttcc ggtatcgctc
      841 atgtatagta ccgagagccg acccgttccg tctgccgtta ccctgtcacc ggttttctga
      901 acgctgctta tatgtgcagt cagcgaacat tttaatactc tttgaatatc ggggaggtat
      961 tcgggcagcg ttatatcaca ctcgataggt tgctccaaag cttcccgaca ggctgttttt
     1021 ctttttgaaa cttcattcgt
//
LOCUS       DBMDVO010000659         1039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66051_length_1039_cov_0.858209, whole genome shotgun sequence.
ACCESSION   DBMDVO010000659 DBMDVO010000000
VERSION     DBMDVO010000659.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66051_length_1039_cov_0.858209"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="ACZPE2_08265"
     CDS             <1..1010
                     /locus_tag="ACZPE2_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIR1319881.1"
                     /translation="EYNLERMDQLLEGTTMKLRPHYKSNKCAEIAKLQIKRGAAGITC
                     AKLGEAEDLANAGIENILIANQVVQPSKVKRVAELAKDHYIIICVDDKDNIKALSDAA
                     VEAGSQIHVLVEYEVGMKRCGVDTYDEFIELAELIDKSEGLIFEGIQAYGGHMAHEHS
                     LETRIAETNRIENEVRELKALLEAKGLKVKEVGGGSTGTIEQKAKDTVYTDIQAGSYI
                     FMDNAYGQLHLGFKNALFVLTTVISIKKDRVVTDTGVKSLGMDQGDPVFLGVPEGSPI
                     SMSEEHGAVYCEHNFKINDKLRYIPGHCCTTVNIFDELYVVDGDEVVDCWKITARGKA
                     Y"
BASE COUNT          335 a          170 c          260 g          274 t
ORIGIN      
        1 tcgagtataa ccttgaacgt atggatcagc ttcttgaagg aacaacaatg aagctcagac
       61 cacattataa atcaaataaa tgtgcagaaa ttgcaaagct tcagattaaa agaggagcag
      121 ccggcataac ctgtgcaaag ctcggtgagg cagaggattt agccaatgca ggcattgaaa
      181 atattcttat tgccaatcag gttgttcagc cgtctaaggt caaacgtgtt gcagagcttg
      241 ccaaagacca ctatataata atctgtgtgg atgataaaga caatattaag gcactttccg
      301 atgcggcagt ggaggccggt tctcaaattc atgtgcttgt tgagtatgaa gtgggaatga
      361 aacgctgcgg agtagataca tatgatgaat ttatagagct tgcggagctt atagataaat
      421 ccgaaggact tatatttgaa ggtatacagg catacggcgg acatatggca catgagcatt
      481 ctcttgaaac aagaattgcc gaaacaaacc gtatcgaaaa tgaggtaaga gagcttaagg
      541 cacttcttga ggctaagggt cttaaggtta aagaagtcgg tggaggaagt acaggcacaa
      601 tagagcagaa ggcaaaggat actgtttata cggatattca ggccggaagt tatattttta
      661 tggataatgc ttatggacag cttcatctcg gatttaaaaa tgcgcttttt gtgcttacaa
      721 cggttatcag cataaagaag gacagagttg ttacagatac aggtgttaaa tcactcggta
      781 tggaccaggg cgatccggtt ttcctcggtg ttcccgaagg cagcccgatt tccatgagcg
      841 aggagcatgg tgcggtatac tgtgagcata attttaagat aaatgataaa ctcagatata
      901 ttcccggaca ttgctgtaca acagtgaata tatttgacga gctttatgtt gttgacggcg
      961 atgaggttgt tgactgctgg aagataacag caagaggcaa ggcttattaa tattacagaa
     1021 aaagctaatt gaagatttt
//
LOCUS       DBMDVO010000660         1037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66169_length_1037_cov_1.441239, whole genome shotgun sequence.
ACCESSION   DBMDVO010000660 DBMDVO010000000
VERSION     DBMDVO010000660.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66169_length_1037_cov_1.441239"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="ACZPE2_08270"
     CDS             <1..720
                     /locus_tag="ACZPE2_08270"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR1319882.1"
                     /translation="DKTIRFTVKSSGGGGGGGGGGGGGGGTSRYTVSFESNGGSKVSN
                     QTVTRNSVMKEPTAPTKENFDFDGWYSDKELKTEYDFSAKVTKSFTLYAKWTEKDNSV
                     NQIILTIGKNAARVFGKTKANDVAPKIEKDRTMLPARFVAENLGAKVEWDGEKQLVTI
                     TGKNLKTDKNVTILITIGSSAAKVNGKEIKLDSPAFIENDRTYTPVRFISEYLGASVE
                     WVEKDQKVIITKPEIKRAETK"
     gene            864..1025
                     /gene="mobC"
                     /locus_tag="ACZPE2_08275"
     CDS             864..1025
                     /gene="mobC"
                     /locus_tag="ACZPE2_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017524.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HIR1319883.1"
                     /translation="MTNRIKENIDYDILCERYSGDILDELLKIYNELNHIGNNINQIA
                     HTANAERHI"
BASE COUNT          347 a          214 c          250 g          226 t
ORIGIN      
        1 gataagacaa tccgctttac tgttaaatcg tccggcggcg gtggcggcgg tggcggcggt
       61 ggcggcggtg gcggcggcac ttcacgctac accgtatcgt ttgaatcgaa cggcggcagc
      121 aaggtttcaa atcagaccgt tacaagaaac agcgttatga aagagccgac ggcgccgaca
      181 aaggaaaact ttgattttga cggttggtac tcggataagg aactcaaaac cgagtatgat
      241 ttttccgcaa aggtaacaaa gagcttcaca ctctatgcaa aatggacgga aaaggataat
      301 tcggtaaatc agattatctt aacaatcggc aaaaatgctg cacgggtatt cggaaaaacg
      361 aaagcaaacg acgttgctcc gaaaatcgaa aaagaccgca caatgcttcc ggcaaggttt
      421 gttgccgaaa acctcggagc aaaggtcgaa tgggacggcg aaaaacagct tgtaaccatt
      481 acgggcaaaa accttaaaac cgataaaaac gtaactattc ttattaccat cggctcatcg
      541 gcagcgaagg taaacggcaa ggaaatcaag cttgattctc ctgcgtttat cgaaaacgac
      601 cgcacctaca cacccgtaag attcatttcg gagtatttgg gcgcaagcgt cgaatgggtt
      661 gaaaaggatc agaaggtaat tatcacaaag cccgaaatca aaagggctga aacaaagtaa
      721 cactaaataa agctttccga aaaggaaatt ggtgacggca cagcaggccg cccggtatag
      781 aggcaaaacg cctttattgc cgggcggctt ttttgtgcct ttttatatga tattacacaa
      841 tcacagcaaa cgagatagat gcgatgacga atcggataaa agaaaatatc gattatgaca
      901 ttctttgcga gaggtatagc ggcgatatac ttgatgagct tttaaagatt tataatgagc
      961 tgaaccacat cggcaacaat atcaatcaga tcgcccatac tgcaaatgct gaaagacata
     1021 tttaagatga tagaatt
//
LOCUS       DBMDVO010000661         1036 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66257_length_1036_cov_1.216043, whole genome shotgun sequence.
ACCESSION   DBMDVO010000661 DBMDVO010000000
VERSION     DBMDVO010000661.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66257_length_1036_cov_1.216043"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="ACZPE2_08280"
     CDS             complement(<1..232)
                     /locus_tag="ACZPE2_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HIR1319884.1"
                     /translation="MDNGLNELQEKIQYRFKNISLLERAVTHSSYANEKHLTTGDNER
                     LEFLGDSVLGFITAEYLFSHHRNFPEGELTRLR"
     gene            complement(251..>1036)
                     /gene="trmFO"
                     /locus_tag="ACZPE2_08285"
     CDS             complement(251..>1036)
                     /gene="trmFO"
                     /locus_tag="ACZPE2_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR1319885.1"
                     /translation="QSRYDRGGSDDYINCPMNKEQYEAFYEAIINAESAETHSFDKRH
                     DVYEGCMPIEVLASRGKDTMRFGPLKPVGLRDPRTGHRPWANLQLRRENSGGTMYNLV
                     GFQTNLKFGEQKRVFSMFPALHDAEFLRYGVMHRNTFINSPRLLNASYSLRSDKRIFF
                     AGQITGVEGYMESASSGIMAGLNAARYLLGKEPLVLPNTTVTGALARYISDETVTNFQ
                     PMGANFGMLPPLPEKIRDKSERYTAIAERGMRDLEEYLKSLGL"
BASE COUNT          240 a          267 c          236 g          293 t
ORIGIN      
        1 agcgtaggcg cgtcagctcg ccctcgggaa aatttctgtg gtgcgaaaaa agatactccg
       61 ccgttataaa gccgagaacc gagtcgccca gaaactcaag tcgctcattg tcgcccgtag
      121 taagatgctt ttcgtttgca tacgacgaat gtgtgaccgc tctttccaaa agcgatatat
      181 ttttaaatct atattgtatt ttttcctgta attcgttaag tccgttatcc attttaaagt
      241 cctttcactc tcacagcccg agacttttaa gatattcctc gaggtctctc atgcctcttt
      301 cggcgattgc cgtataacgc tcgctcttgt cccttatctt ttcggggagc ggcggaagca
      361 taccgaaatt ggcccccata ggctgaaaat tcgtcacggt ttcatcgctt atatatctcg
      421 cgagagcacc cgtgacggtg gtattcggaa gcacaagcgg ctcttttccc aaaaggtacc
      481 ttgcggcgtt cagtcccgcc ataattccgg aggaggccga ttccatatag ccctctactc
      541 cggttatctg accggcaaaa aatattcttt tgtccgagcg cagagaatat gaggcgttta
      601 aaagccgcgg tgaatttata aacgtgtttc tgtgcataac tccgtagcgg agaaattccg
      661 catcgtgaag tgccggaaac atggaaaaca ctcttttttg ctcgccgaat ttgagatttg
      721 tttgaaatcc gacaagatta tacatcgtac caccgctgtt ttcacggcga agctgaagat
      781 ttgcccacgg tctgtgtccc gttctcgggt cgcgaagccc tacgggcttt aacggcccga
      841 agcgcatggt gtcttttccg cgtgatgcca aaacctcaat aggcatacag ccctcataca
      901 catcgtgccg cttgtcaaag gagtgcgttt cggcactctc ggcatttata attgcttcgt
      961 aaaacgcttc atactgctct ttattcatgg ggcagttaat gtaatcgtcg ctgccgccgc
     1021 ggtcatatct cgactg
//
LOCUS       DBMDVO010000662         1033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66496_length_1033_cov_1.287554, whole genome shotgun sequence.
ACCESSION   DBMDVO010000662 DBMDVO010000000
VERSION     DBMDVO010000662.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66496_length_1033_cov_1.287554"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="ACZPE2_08290"
     CDS             complement(<1..410)
                     /locus_tag="ACZPE2_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025802.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HIR1319886.1"
                     /translation="MDYRGQHCPICGKEFCDGDDIVVCPECGTPYHRECYKTAGHCVN
                     EERHGENFEWKPETAPHAKEKTPNVGQNANDDGHKVVFCPVCGRENPAEEPNCLNCGA
                     RLYNNQNGGKAFIPPVELPNMDKQPFGKVVNISP"
     gene            complement(462..>1033)
                     /locus_tag="ACZPE2_08295"
     CDS             complement(462..>1033)
                     /locus_tag="ACZPE2_08295"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1319887.1"
                     /translation="RNPMRSERITSLSRYVMADVINPSVTAGTQWLERSLDDSANKRI
                     SIAEGFLATDAILELYINIVSGLVVYPKMIEKHLNDELPFMATENILMEAVKKGGNRQ
                     TLHEEIRRHSMAAGAVVKVEGKPNDLVERIAADPMFSLTLDEIKAQLKPENFIGRAPQ
                     QVTEFIEGDVKPVLEKYKDLLGLEVEIKV"
BASE COUNT          213 a          285 c          238 g          297 t
ORIGIN      
        1 atgggcgaaa tattaactac cttgccgaac ggctgtttat ccatattcgg aagctcaacg
       61 ggcggaataa acgccttgcc gccgttttga ttgttataga gccttgctcc gcaattaagg
      121 cagttcggct cctccgccgg attctctctg ccgcacaccg ggcaaaaaac caccttgtga
      181 ccgtcatcgt ttgcattttg accgacattc ggggtcttct cttttgcatg gggcgcagtc
      241 tccggcttcc actcgaagtt ctcgccgtgt cgctcttcgt ttacgcaatg ccctgcggtc
      301 ttatagcatt ctctgtggta cggcgttccg cattccgggc agacgactat atcgtcgccg
      361 tcgcaaaact ccttaccgca tatgggacaa tgctgtcctc tgtaatccat ctaaaatcac
      421 ccttgtgaaa gtattttact ttctcccgct tacaacttat gtcagacctt gatttcaacc
      481 tcaagtccca aaaggtcctt gtatttttcg agaaccggct ttacgtcgcc ctcgataaat
      541 tccgttacct gctgcggtgc tctgccgatg aagttttcgg gctttaactg tgccttgatt
      601 tcgtcaagcg tgagcgagaa catcgggtcg gcagcaattc tttcaactaa gtcgttgggc
      661 ttgccttcaa cctttaccac cgcacctgcc gccatggagt gacggcgaat ttcctcgtgg
      721 agagtctggc ggttgccgcc ctttttgacc gcttccataa ggatattttc cgtagccata
      781 aacggaagct catcgtttaa atgcttttct atcatcttgg gatatacgac gagtccgctg
      841 acaatgttga tgtaaagctc caaaatagcg tctgtcgcaa ggaagccctc ggctatgctt
      901 attcgcttgt ttgccgagtc gtcaagagat ctctcaagcc actgcgtgcc ggccgtaacc
      961 gacgggttta tgacatctgc cataacgtag cgcgaaagag acgtgattct ctcgcttctc
     1021 atgggattgc gct
//
LOCUS       DBMDVO010000663         1032 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66596_length_1032_cov_1.127820, whole genome shotgun sequence.
ACCESSION   DBMDVO010000663 DBMDVO010000000
VERSION     DBMDVO010000663.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66596_length_1032_cov_1.127820"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="ACZPE2_08300"
     CDS             <1..717
                     /locus_tag="ACZPE2_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1319888.1"
                     /translation="VSFKIIGVTDMMSSFGGDSEKMLENMNSLTGGAAVTSAMIMVPS
                     TVATQIMGGEARYDTVYIMAKDENDLESAGNAAANRIKARHNNMDRDCYTVTNMATYI
                     DLLDTVINVFTIFIAAVSAISLVVGGIGVMNIMLVSITERTREIGIRKALGAKTSTIL
                     FQFLTESIILCLIGGLIGLLLGVSGAALVSKIMNVPLEVKFSTIALAIGFSTAIGVIF
                     GVYPAKRAAKMPPIEALRRD"
BASE COUNT          291 a          205 c          239 g          297 t
ORIGIN      
        1 gtgagcttta agattatcgg agttaccgat atgatgagct cgttcggcgg cgacagcgaa
       61 aagatgctgg aaaatatgaa ttctcttacc ggcggtgcag cggtgacgag tgccatgata
      121 atggttccgt ctaccgtagc cactcaaata atgggcggcg aggctcgata cgataccgtt
      181 tatattatgg caaaggatga aaacgatctt gaaagtgccg gcaatgcggc agcaaaccgt
      241 ataaaggcaa gacataacaa catggaccgc gattgctata ccgtaaccaa tatggcaacc
      301 tatattgacc ttttagatac ggtcataaac gtatttacca tattcatagc ggcggtcagc
      361 gcaatatctc tcgtagtcgg cggaataggc gttatgaata ttatgctcgt ctccataacg
      421 gagcgaacgc gcgaaatagg catacgaaag gctctcggag ccaaaacgtc cacgatattg
      481 ttccagtttc tcacggagtc cataattctg tgcctaatag gcggacttat agggcttttg
      541 ctgggtgttt cgggagcggc actggtttcg aaaattatga atgttccgct tgaggtgaaa
      601 ttctcgacaa ttgcgcttgc cataggattt tcaacggcga taggtgtgat attcggcgtt
      661 tatcccgcaa agcgagccgc aaaaatgccg cctatcgagg cacttcgaag agactgattt
      721 ttttaaacgg agttgtgggc agagatgacc cacagccccg tttttttgtt tgtacttaaa
      781 aattgtctct cgatgggtca tatcctgcaa aacggtattg tatatttatt tttgtctaaa
      841 acttcgcgac aagatcgtta ctttacattt ttgagagcgt aaaatccgct taaaaccaaa
      901 gtttttaaca ttatgaacac ggttttgaac attttgattg tcaaaaactt ccgtggcata
      961 atatgtcaaa actgcataat tgtattgttc gaaataaatt tattgcatat ttttttgaat
     1021 tacaaggttc gc
//
LOCUS       DBMDVO010000664         1032 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66621_length_1032_cov_0.825994, whole genome shotgun sequence.
ACCESSION   DBMDVO010000664 DBMDVO010000000
VERSION     DBMDVO010000664.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66621_length_1032_cov_0.825994"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="ACZPE2_08305"
     CDS             complement(<1..339)
                     /locus_tag="ACZPE2_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319889.1"
                     /translation="MENGEKLFDTFGEYLEKCGDVEFDFFCDDLYCDHTVVWNSFNNR
                     LTEKAKEKWNKLLGSKYEVTYSDDKYRIRQINIPNENLRKSCLDFAYAQAGYIPASEY
                     DELFCSENRIC"
     gene            complement(355..>1032)
                     /locus_tag="ACZPE2_08310"
     CDS             complement(355..>1032)
                     /locus_tag="ACZPE2_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319890.1"
                     /translation="ESGITLYLNDEDTEVRAEILPDVGECIQDVYIYDVYDAEKRKVV
                     KDKPNYTYNCSAVQLAYIIDTDVFAEEYGFDFPTIVTTTGTEIKHDDGVLTLMIEYSC
                     LDRLETISEQDIGLSLYDVYDKDEKIVIGYAHSGKHSKEKDEGLSATGWSHTFGSEII
                     PHLCVKTAFVDGEVTPPELDNPHIYDGLSVRFVGAKVNPLLWFKAYRTDVNKDLLDSI
                     SAIQVKK"
BASE COUNT          259 a          259 c          184 g          330 t
ORIGIN      
        1 gcaaatgcgg ttttcgctac aaaacagctc atcgtactct gatgccggaa tatatcctgc
       61 ctgtgcatac gcaaaatcaa ggcaggattt acgcagattt tcattgggta tgtttatctg
      121 tctgatacga tacttatcgt cggagtaagt aacttcatat tttgagccga gcagtttgtt
      181 ccatttttcc tttgcttttt ctgtcagcct gttattgaag ctgttccata ctacagtatg
      241 gtcacaatac aagtcatcgc agaaaaaatc aaattccaca tcaccgcatt tttcaagata
      301 ttcaccgaat gtatcaaaca gcttttctcc attttccata atcaaattct ccttttattt
      361 tttcacctgt atcgcactta tgctgtcaag caggtccttg ttaacgtccg tcctgtaagc
      421 cttaaaccaa agcaaaggat ttacttttgc tccgacaaaa cgcaccgata agccgtcata
      481 aatatgcgga ttatcaagct ccggaggcgt tacctctccg tctacaaacg cagttttgac
      541 gcacaaatga ggtattatct cacttccgaa tgtatggctc cagcctgtcg ctgataagcc
      601 ctcgtccttt tccttgctgt gtttgccgga gtgagcatag cctattacta tcttctcgtc
      661 tttgtcgtat acgtcataca gtgataatcc tatgtcctgc tccgagatag tttcaagcct
      721 gtcaagacag ctgtattcta tcatcagcgt cagaacacca tcatcgtgct ttatctccgt
      781 acctgtggtt gttacgatag tcggaaaatc aaagccgtat tcctctgcaa acacgtccgt
      841 atcaattatg tacgcaagct gtaccgccga gcagttgtat gtataatttg gcttatcttt
      901 aaccaccttg cgtttttctg cgtcataaac gtcatagata tacacatcct gtatgcactc
      961 gcctacatcg ggaagtatct ccgctctgac ctcggtatcc tcgtcattca gatacagcgt
     1021 tatacccgat tc
//
LOCUS       DBMDVO010000665         1031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66710_length_1031_cov_0.807527, whole genome shotgun sequence.
ACCESSION   DBMDVO010000665 DBMDVO010000000
VERSION     DBMDVO010000665.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66710_length_1031_cov_0.807527"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="ACZPE2_08315"
     CDS             <1..767
                     /locus_tag="ACZPE2_08315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1319891.1"
                     /translation="DVCAMIIKFKRPAAIFCGIILMVFGLLFLLYAVFGMNGDEEIRA
                     KKTIAQHDTSVDPEKPMVALTYDDGPYTPVTGRILESLKAVGGRATFFVVGSRIDGRE
                     EITKKITEYGCEIGNHTYGHVVLTKTDNENALRELAKNDEVIFDTVGIKPSVVRPPCG
                     CYNDFIRQNEKRPLVMWTIDTRDWSHQNKDRTVNTVLSSVKDGDIVLMHDLFVPTAEA
                     SEILIPELVNRGFQLVTVSELINYRGEINGTVLNNS"
     gene            837..>1031
                     /locus_tag="ACZPE2_08320"
     CDS             837..>1031
                     /locus_tag="ACZPE2_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319892.1"
                     /translation="MKKILSILLAAALIVCTFAACGNKKTDTSSDLAYVKDKGTLVIG
                     ITLFAPMDYYEEGNTTDLKGF"
BASE COUNT          316 a          209 c          244 g          262 t
ORIGIN      
        1 cggatgtgtg cgccatgata ataaaattca aaaggcctgc agcgattttt tgcggaatta
       61 ttctgatggt attcggactg ttgtttttgc tttatgccgt tttcggaatg aacggtgacg
      121 aagaaataag agcaaaaaag actatagctc agcacgatac gtccgttgat ccggaaaagc
      181 cgatggttgc cctgacgtat gacgacggtc cgtacactcc cgtcaccgga agaattctcg
      241 aaagcctcaa ggcggtgggc ggcagagcaa ctttttttgt tgtcggcagc agaatagacg
      301 gtcgcgagga aataacaaag aaaataacag aatacggctg cgaaataggc aatcacactt
      361 acggacatgt cgtgcttacc aaaacagaca acgaaaacgc tttacgcgag cttgcaaaaa
      421 atgatgaggt gatttttgat accgtcggaa taaagccaag cgtcgtaaga cctccgtgcg
      481 gctgttataa tgattttata agacaaaacg agaaaagacc gctcgtcatg tggacgattg
      541 acacaagaga ctggagtcat caaaacaagg accgcacggt aaatacggtg ctatcaagcg
      601 tcaaggacgg agatatcgtt ttgatgcacg atttatttgt ccctacggcg gaagcgtcgg
      661 aaatactgat tcccgagctt gtgaatcgcg gctttcagct tgtcaccgtg agtgaactca
      721 tcaattatcg cggcgagata aacgggacag ttttaaataa cagttgacaa accgagttca
      781 ataagttatc atattagcat attaacactt taccacggta aaacacggag aacgttatga
      841 aaaagatttt aagtattttg cttgccgctg ccttgattgt atgcacattt gccgcttgcg
      901 gcaacaaaaa aaccgatact tcttcggacc ttgcatacgt taaagataaa ggaacgcttg
      961 ttatcggcat tactcttttt gctcctatgg attattatga agagggtaat accacagacc
     1021 ttaagggctt t
//
LOCUS       DBMDVO010000666         1031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66711_length_1031_cov_0.795699, whole genome shotgun sequence.
ACCESSION   DBMDVO010000666 DBMDVO010000000
VERSION     DBMDVO010000666.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66711_length_1031_cov_0.795699"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            233..>1031
                     /gene="proS"
                     /locus_tag="ACZPE2_08325"
     CDS             233..>1031
                     /gene="proS"
                     /locus_tag="ACZPE2_08325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1319893.1"
                     /translation="MKLDKLVGERFKEKPSDCVIDSHALMLRGGYMKGVANGIYSQYT
                     PLNRICRKIEDILRDEMDKVGGQEVKFPVVLPASLWDESGRYESVGSELMRFTDRNNA
                     RLVLGMTHEEASVQLVREYANSYTKYPFMIYQIQTKFRDEARPRAGLIRVREFTMKDA
                     YSFHTSQEDLEEYYAEVYEAYNRIFARAGLPEVVAVKSDSGMMGGSVSHEYMLLTPVG
                     EDTIAYCSDCDYRANMEAAASITENKKDGDDEELKLVETPNIHTIEDI"
BASE COUNT          263 a          219 c          291 g          258 t
ORIGIN      
        1 acagtagata gtatttgtcg cttcagagag cgggcggtcg gtgaaagccc tgcggcgtta
       61 ttatcgaacc cgtctttgag ctccgcgcag aacgcggcgg gcgtcccgtt acagacaaat
      121 gagagcggat tttccgaata caggtggtac cgcaggctat tttgttagtt tgtcctgaat
      181 gaagctatag cttcgttcgg gatttatttt ttgttgaacc ggaggattat gtatgaaact
      241 tgataagctc gtaggcgaga gattcaaaga aaagccgtcc gactgcgtta ttgacagcca
      301 tgcgctgatg ctgcgcggcg gatatatgaa gggcgtcgct aacggtattt attcgcagta
      361 tacaccgctt aaccgtatat gccgtaaaat cgaggatata cttcgcgacg agatggataa
      421 ggtaggcggc caggaggtta aattcccggt agtgcttccc gcaagtcttt gggacgaatc
      481 gggcagatat gagagcgtcg gcagcgagct tatgcgcttt accgaccgta ataacgctcg
      541 tcttgtcctc ggtatgactc atgaggaggc gtcggtgcag cttgttcgcg agtatgcaaa
      601 ttcatataca aaatacccgt ttatgatata tcagattcag acgaagttcc gcgacgaggc
      661 aagaccgcgc gcagggctta tccgtgttcg tgaatttacc atgaaggacg catattcctt
      721 ccatacttcg caggaggatc ttgaggagta ctacgctgag gtctacgaag catataaccg
      781 catttttgca cgtgccggtt tgccggaggt agttgccgta aagtcggatt ccggtatgat
      841 gggcggcagc gtttcgcatg aatatatgct cttaactccg gtaggcgagg atacaatagc
      901 ctattgctcc gattgtgatt acagagcgaa catggaggca gcggcgagca ttacggaaaa
      961 caaaaaggac ggtgatgacg aggagctgaa gcttgtcgaa acaccgaata tccatacaat
     1021 agaggatatt t
//
LOCUS       DBMDVO010000667         1030 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66793_length_1030_cov_0.919268, whole genome shotgun sequence.
ACCESSION   DBMDVO010000667 DBMDVO010000000
VERSION     DBMDVO010000667.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66793_length_1030_cov_0.919268"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="ACZPE2_08330"
     CDS             <1..210
                     /locus_tag="ACZPE2_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319894.1"
                     /translation="FFTTLVGVLIYGALFTFVNPLAAAIIVVVIGLCVTCYPPQFYAA
                     RVIERSILKDMKEKQPKVEYIGEEE"
     gene            210..>1030
                     /locus_tag="ACZPE2_08335"
     CDS             210..>1030
                     /locus_tag="ACZPE2_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1319895.1"
                     /translation="MVLGEYDVAVIGGGHAGCEAALAAARLGMKTVMFSISLDAIGNL
                     PCNPSIGGTAKGHLVRELDALGGEMGKAADVSFIQSKMLNKGKGPAVHSLRAQIDRKK
                     YHVEMKKRVENQENLQVKQAEIVNVITDEDNNVTEVVTHTGVEYKVKAVIIASGTYLK
                     GKILIGSYSRESGPDGMFPANELSENLRRLGIELKRFKTGTPARVHADTLDYSKMELE
                     EGDEKIVPFSFETENVGKNLVPCYLVYTNEKTHQIIHDNLGRSAMYGGMVEGIGPR"
BASE COUNT          320 a          175 c          262 g          273 t
ORIGIN      
        1 ttttttacaa cgcttgtagg agtacttata tacggtgcgt tgtttacatt cgttaatccg
       61 cttgcagcgg caattatagt agtagttatc ggactttgcg ttacttgcta tccgccgcaa
      121 ttttatgccg caagagtaat tgaacgctca atattaaagg atatgaaaga aaaacagcct
      181 aaggttgaat atatcgggga ggaagaataa tggttttagg tgaatatgat gttgccgtaa
      241 tcggcggcgg tcacgcgggc tgtgaggcgg ctttggcggc ggcaagattg ggtatgaaaa
      301 cagttatgtt ttcaataagc cttgacgcaa taggaaatct cccttgtaat ccaagtatag
      361 gcggtacggc gaaaggacac cttgttcgtg agcttgacgc actcggcggt gaaatgggta
      421 aagcggcgga cgtttcgttt atacagtcga aaatgcttaa taaaggcaaa ggccctgccg
      481 tacattcgct tagagcacag attgacagaa aaaagtatca cgttgaaatg aaaaaacgtg
      541 ttgaaaatca agagaattta caggttaaac aagcggaaat cgtaaatgta attaccgatg
      601 aagataataa tgtaacggaa gttgtaacac atacaggcgt tgaatataag gtaaaagcgg
      661 ttattattgc cagcggaact tatttaaagg gtaaaattct tatcggaagt tattcgcgtg
      721 aaagcggtcc tgacggaatg ttccctgcaa atgaactttc ggaaaatctt cgcagattgg
      781 gtattgaatt aaagcgattt aaaacaggta ctccagcaag agtgcacgcg gatacacttg
      841 actacagtaa gatggagctt gaggagggtg acgaaaaaat cgtgccgttc tcgtttgaaa
      901 cggagaatgt cggcaaaaat cttgttcctt gctatcttgt gtatacaaat gaaaaaactc
      961 accaaattat acacgacaat ctcggtcgtt cggcaatgta cggcggtatg gttgagggta
     1021 taggtccgcg
//
LOCUS       DBMDVO010000668         1028 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_66944_length_1028_cov_0.941748, whole genome shotgun sequence.
ACCESSION   DBMDVO010000668 DBMDVO010000000
VERSION     DBMDVO010000668.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66944_length_1028_cov_0.941748"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="ACZPE2_08340"
     CDS             complement(<1..278)
                     /locus_tag="ACZPE2_08340"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF014043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR1319896.1"
                     /translation="MRNCPNADIDVLRHILKYCSNIAELISRFGNDIEVFKADLAFRD
                     SVSMNILQIGELTGHLSEEYRQSTKSHMPWPAIKAMRNLFAHNYGQMN"
     gene            complement(262..594)
                     /locus_tag="ACZPE2_08345"
     CDS             complement(262..594)
                     /locus_tag="ACZPE2_08345"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1319897.1"
                     /translation="MSDPVYTVEQIADIVAPIAAKYDVSRLYLFGSYARGDADEKSDI
                     DLRVDGGQLGNLFALGGFYADLEEALKKSLDLVTTDTLRQNKSDIMTRNLIRNMRKDE
                     KLIYEKLS"
     gene            670..981
                     /locus_tag="ACZPE2_08350"
     CDS             670..981
                     /locus_tag="ACZPE2_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319898.1"
                     /translation="MEEITVVIPVNFTDAGRLFGLFEIRNAVEAVLICVPLAALLMRI
                     LPFGLLVRIGIVSAVTVIIGGFALIGIGNMSLLEFLKQYIRFKRQAKILTYKGGVKNE
                     P"
BASE COUNT          288 a          212 c          227 g          301 t
ORIGIN      
        1 aaattcatct gaccgtaatt gtgcgcgaaa agatttcgca tagcctttat agcgggccat
       61 ggcatatggc tctttgtgct ttgcctgtat tcttcggaaa gatgaccggt aagctcgcct
      121 atctgaagta tattcatact gaccgaatct ctgaaagcaa ggtcggcttt aaaaacctct
      181 atgtcgtttc cgaaacggga aataagctcg gctatattgc tgcagtattt aagaatatga
      241 cgaagaacat ctatatccgc attaggacag tttctcataa atcagctttt catcctttcg
      301 catattccgt attaaattcc gcgtcataat atcgctttta ttctggcgca gcgtgtcggt
      361 ggtaacaagg tcgagggatt ttttaagcgc ttcttcaaga tcggcataaa atccgccgag
      421 agcaaacagg ttaccgagct gtccgccgtc tacgcgcaga tctatgtcgc ttttttcatc
      481 tgcgtcgccg cgggcatacg agccgaataa atatagcctt gaaacatcat attttgccgc
      541 tattggtgca acaatatccg ctatttgctc taccgtataa accgggtcgc tcataataca
      601 taccaccttt cattttcagt atatcacaaa caaaaaaaat cttcaagtat aagtatagga
      661 ggaaatttaa tggaagaaat aaccgtagta atacccgtaa attttaccga cgccgggcgg
      721 ctgttcgggc tttttgaaat acgcaatgct gtagaggcag tgcttatctg cgtgcctttg
      781 gcagcgcttt taatgcgcat attgccgttc ggtcttttag tgcgtatagg aattgtaagc
      841 gccgtcaccg ttattatagg cgggtttgcg ctaataggca tcgggaatat gagccttttg
      901 gagtttttaa agcagtatat ccgctttaaa cggcaggcga aaattttaac atataaaggt
      961 ggtgtaaaaa atgaacctta aaaatattct gtttggaaac ggagataaaa atacggagga
     1021 cggcacgg
//
LOCUS       DBMDVO010000669         1027 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67039_length_1027_cov_0.911447, whole genome shotgun sequence.
ACCESSION   DBMDVO010000669 DBMDVO010000000
VERSION     DBMDVO010000669.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67039_length_1027_cov_0.911447"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..100)
                     /locus_tag="ACZPE2_08355"
                     /pseudo
     CDS             complement(1..100)
                     /locus_tag="ACZPE2_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            125..415
                     /locus_tag="ACZPE2_08360"
     CDS             125..415
                     /locus_tag="ACZPE2_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016154.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HIR1319899.1"
                     /translation="MQYAIVETDGTLSVLLKKENQPATKSELKINTPKSSLPCIVISD
                     GRIIKTDFAECNTDSDKIKAILKDKHLNEEDVMLMTLDKDGNINILKKDKEK"
     gene            412..792
                     /locus_tag="ACZPE2_08365"
     CDS             412..792
                     /locus_tag="ACZPE2_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HIR1319900.1"
                     /translation="MKRLIIAVCMLVTGIAISIGGYVNLKSICRNMSCSVNEVIRAAE
                     NGDADETKRKCDDTEKMWDKKSPLLGVYTNHSEMDELMMLMKQIRQLSTDRDYDELIE
                     KCRESIYRFEHIAETETPSFGNVF"
     gene            complement(789..>1027)
                     /locus_tag="ACZPE2_08370"
     CDS             complement(789..>1027)
                     /locus_tag="ACZPE2_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1319901.1"
                     /translation="IVGGLSTLIFIFNPAVIILGGGIMNEDYIISEIDKRIHSKDIRS
                     FKTVQIKKAMLKNRAGMLGAAYLAKKNFDKLPKN"
BASE COUNT          343 a          203 c          233 g          248 t
ORIGIN      
        1 cttctatacc atcgagaacg gcagcatagt agtgggtcac cagattaggt acgatgacac
       61 ctatcacgcg cggtgcttcc ttgcggaggc tctgcgcaaa aaagatattt ttgatatatc
      121 ggaggtgcaa tatgccatag ttgagacgga cggcacgtta agcgttctgc tcaaaaaaga
      181 aaatcagccc gccacaaaga gcgagctgaa aataaatact ccgaaaagct ctcttccgtg
      241 tatagtcata agcgacggaa gaataatcaa aaccgatttt gccgaatgta ataccgacag
      301 cgacaaaata aaagctatcc taaaggacaa gcatctaaac gaagaagatg taatgcttat
      361 gacacttgac aaggacggca acataaatat attaaaaaag gacaaggaaa aatgaaaaga
      421 ctgataattg ccgtatgtat gctcgttacg ggaatagcga tttcgatagg cgggtacgta
      481 aatctgaaaa gtatttgccg caatatgagc tgctcggtaa acgaggtcat aagagcggcg
      541 gaaaacggtg acgccgacga gacgaaaaga aaatgcgacg atacggaaaa aatgtgggat
      601 aaaaaaagtc cgctgctcgg cgtttacacc aatcacagcg aaatggatga gcttatgatg
      661 cttatgaagc agatacgaca gctcagtaca gatagagatt acgatgagct tattgaaaaa
      721 tgccgcgaga gcatttaccg tttcgagcac atagcagaaa cggaaacgcc ctcgttcgga
      781 aatgtttttt agtttttcgg cagcttatcg aaatttttct ttgcaagata tgccgctccg
      841 agcattcctg cgcgattttt gagcatggct tttttgattt gcacggtttt aaagcttctt
      901 atatcctttg aatgaatacg cttatcaatc tcggagatta tataatcctc attcataata
      961 ccgccgccga gaattatgac agccggatta aatatgaaaa tgagcgtaga caaaccgccc
     1021 actattt
//
LOCUS       DBMDVO010000670         1027 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67051_length_1027_cov_0.747300, whole genome shotgun sequence.
ACCESSION   DBMDVO010000670 DBMDVO010000000
VERSION     DBMDVO010000670.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67051_length_1027_cov_0.747300"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="ACZPE2_08375"
     CDS             complement(<1..325)
                     /locus_tag="ACZPE2_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIR1319902.1"
                     /translation="MKIFKIIATLVGCVIAPVVSVFLSYSLDVMLTTQELKLFDFNTC
                     LEGLKVNQKQQQLFMIFTALLIGLIIFVVFVAMNNKYKADTITVTPKIKIPVPAGEGQ
                     NGSARF"
     gene            complement(392..>1027)
                     /locus_tag="ACZPE2_08380"
     CDS             complement(392..>1027)
                     /locus_tag="ACZPE2_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HIR1319903.1"
                     /translation="EVEKANIEGKNYTPTSEESEPLRFTFNMRNGANGSVYGNMCIND
                     DLLVHNISVKDRLDAETGNVVKFISLPSKQADNGQWWSVVRPSNDTVKEAINHYGISA
                     IGKIAVETVGNIKYKDLKEKAPELSFYRHQNVNYGKAVGEELSKAGIMWSGKVEKTGM
                     VITISADENDSYSKIITEMRERNKAQTAEQAQTAGKVDESAFFDMNAPEPF"
BASE COUNT          256 a          246 c          195 g          330 t
ORIGIN      
        1 taaaccgtgc cgagccgttc tgaccttcgc ctgcaggaac gggtatcttg attttcggcg
       61 tgaccgttat tgtatcagcc ttatacttgt tgttcatcgc aacaaacaca acaaagataa
      121 tcagacctat aagcagtgct gtaaatatca taaagagctg ctgttgtttc tgattgactt
      181 tcagaccttc aaggcaggtg ttgaagtcaa acagtttcag ctcctgtgtt gtcagcataa
      241 catcaagact gtatgataaa aatacagata caacaggtgc aatcacacat ccgacaagag
      301 ttgcaatgat tttgaaaatt ttcattttgc ctcctgtaat gtaaaagccg ggactgcttt
      361 cacaatcccg gtaaattaca tattatttgt ttcagaaagg ctcaggtgca ttcatatcaa
      421 aaaatgcact ttcgtcaacc tttcccgctg tctgtgcctg ctctgctgtc tgagccttgt
      481 tacgctcacg catttcagtt attatcttcg aataggaatc gttctcgtct gcggatattg
      541 tgatgaccat accggtcttt tcgaccttac cgctccacat aatacctgct tttgacaatt
      601 cttctccgac agccttgccg taattcacgt tctgatgacg gtaaaaagaa agctccggtg
      661 ctttttcctt caaatcttta tacttgatat tgccgacggt ttcaacggca attttgccga
      721 ttgcggatat gccgtaatgg tttatagctt ccttaaccgt atcgtttgat ggtcttacaa
      781 cgctccacca ctgaccgttg tcagcctgct ttgacggcag agaaataaac ttcacgacat
      841 tgccggtttc tgcatccagc ctgtccttaa cacttatgtt atgtacaagc agatcatcgt
      901 tgatgcacat atttccgtat acgctaccgt ttgcaccgtt tctcatattg aaagtaaatc
      961 tgagcggctc actttcctcg cttgtgggcg tgtaattctt accttcgata ttggcttttt
     1021 caacctc
//
LOCUS       DBMDVO010000671         1024 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67279_length_1024_cov_1.320693, whole genome shotgun sequence.
ACCESSION   DBMDVO010000671 DBMDVO010000000
VERSION     DBMDVO010000671.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67279_length_1024_cov_1.320693"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="ACZPE2_08385"
     CDS             complement(<1..420)
                     /locus_tag="ACZPE2_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HIR1319904.1"
                     /translation="MDGKKRVKLEELLPIIEEKLSAGGTVVLPITGTSMLPLLVAGRD
                     KVTLKSAVLPLEKDAIPFYRRNDGAFVLHRKVGEDENGYVMCGDNQWVREHGITDKNI
                     IGEVAFITRKGKTFSVDSRRYRLYVKLWKFLFPVRKYI"
     gene            complement(425..691)
                     /locus_tag="ACZPE2_08390"
     CDS             complement(425..691)
                     /locus_tag="ACZPE2_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR1319905.1"
                     /translation="MKIKDGFMLSRVAGSYVVVPVGAVQADFTGMITLNPVGAFLWGK
                     LEKETTRDELLKSVLSQYDTDEKTAAQDIDRYLEKLRKNGLLEE"
     gene            complement(716..>1024)
                     /locus_tag="ACZPE2_08395"
     CDS             complement(716..>1024)
                     /locus_tag="ACZPE2_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319906.1"
                     /translation="GTPFSGKNDESRNAAAPIRAVVFIERSQKNSIEKISPASAVPLF
                     MSQTVRPKTPQYMGVLLSLLDALLKEVPVFLLKCNMDPSAAHVSYDGIEEYYRQNPRA
                     "
BASE COUNT          229 a          286 c          214 g          295 t
ORIGIN      
        1 tatatatttc cttacgggaa acagaaattt ccaaagctta acatagaggc gatatctacg
       61 gctgtccacg gaaaaggttt ttcccttacg cgttataaac gcaacctcgc ctattatgtt
      121 cttatcggtt atgccgtgct ccctgaccca ttgattgtcg ccgcacataa cgtatccgtt
      181 ttcgtcctca ccgacttttc tgtgcagcac aaatgcgccg tcgtttcggc ggtaaaacgg
      241 aatagcatct ttttcaagcg gcaaaacggc acttttaagc gtaaccttgt ctctgccagc
      301 cacaagcagc ggcaacatgc tcgttcccgt aatcggtaaa acgaccgttc cgccggctga
      361 gagcttctcc tcgattatcg ggagcagctc ctctaattta actctttttt tgccgtccaa
      421 aatatcattc ctctaaaagt ccgtttttgc ggagcttttc aagatagcgg tcaatatcct
      481 gcgccgcggt cttttcgtct gtatcgtact gtgagagcac gcttttcaaa agctcgtcgc
      541 gcgtagtttc cttttcgagc ttgccccaca aaaatgcgcc gacgggattc agggttatca
      601 tgccagtgaa gtccgcctga acggctccga ccggcacaac cacatagctt ccggcaaccc
      661 tacttagcat aaagccgtct ttaattttca tataccagca ctgcctttct ttttcttaag
      721 ctctcggatt ttgtctgtaa tattcctcta ttccgtcata cgacacgtgc gccgccgacg
      781 ggtccatatt gcatttgagc aaaaagaccg gcacctcttt taaaagagcg tcgagcagcg
      841 ataacagcac acccatatac tgcggtgttt tcgggcgcac ggtctgtgac ataaacagcg
      901 gtacggcact cgccggactt attttttcaa tactgttctt ttggcttctc tctataaaca
      961 caacagccct tatcggggcc gcggcgtttc tgctctcgtc gtttttgccg ctgaacggtg
     1021 tacc
//
LOCUS       DBMDVO010000672         1023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67371_length_1023_cov_1.060738, whole genome shotgun sequence.
ACCESSION   DBMDVO010000672 DBMDVO010000000
VERSION     DBMDVO010000672.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67371_length_1023_cov_1.060738"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="ACZPE2_08400"
     CDS             complement(<1..947)
                     /locus_tag="ACZPE2_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR1319907.1"
                     /translation="MGKLSDKLAGFFGRLFPMSRNKTIKLNNEIRQTESEHFAQLCEM
                     LGFLENSLNTCTDICRDNAEQIKLCRQSLRTLEDNQVKFGESTASKLKEIKFDIENLK
                     NQNECIGEKLSNEISEKSAGISGELIGCISHHTEEILGCITDSDKRSSDSDKHIVELI
                     ENGISENNKHAGEIKAFNNSLSADIKSLSDSVTAGFDKTSKTGEYADTLNGLSAYNES
                     ISADIKSLSDSVTAGFDKTSKTGEYVSVLNGLSADNESISADIKSLSDSVTAGFDKTS
                     KTGEYVAVLDGLSADNESISADIKSLSDSVTAGFDKTSKT"
BASE COUNT          243 a          229 c          205 g          346 t
ORIGIN      
        1 gttttcgagg ttttatcaaa gcctgcggtt acgctgtccg aaagtgattt aatatcagcc
       61 gaaatgcttt cgttatctgc cgataagccg tcaagcacag ccacatattc gcccgtcttt
      121 gaggtcttgt caaagcctgc ggttacgctg tccgaaagcg acttaatatc agccgaaatg
      181 ctttcgttat ctgctgataa gccgttaagc acagacacat attcgcccgt ctttgaggtc
      241 ttgtcaaagc ctgcggttac gctgtcggaa agcgacttaa tatcagccga aatgctttcg
      301 ttatatgccg ataaaccgtt aagtgtatcc gcatattcgc ccgtctttga ggtcttgtcg
      361 aagcctgcgg taacgctgtc ggaaagtgat ttaatatcag cggaaagcga gttgttgaac
      421 gcttttattt ctcctgcgtg cttattgttt tcagatatgc cgttttcaat aagttcgaca
      481 atgtgcttat cgctatcgga agaacgcttg tcgctgtcgg ttatacaacc gagtatttcc
      541 tctgtgtgat gtgaaataca gccgataagt tcaccggata ttccggcaga tttctctgat
      601 atttcgttac tcagtttttc gccgatacac tcattttgat ttttcagatt ttcaatatca
      661 aattttattt ctttgagttt tgatgcggta gactcaccga atttaacctg attatcttcc
      721 aatgtacgca acgattgacg gcacagtttt atctgctccg cattatcacg gcatatatcc
      781 gtgcaagtat tcaggctgtt ttcaagaaaa ccgagcattt cacaaagctg tgcaaaatgc
      841 tcgctttctg tctgccttat ctcgttgttg agctttattg tcttgttgcg tgacatcgga
      901 aataatcttc cgaaaaatcc cgcaagttta tcggataatt tacccattat ttccaaatct
      961 tccagggagc attgttgttg ttccacattt tctcaagctg ttctttttct cttacagtat
     1021 cca
//
LOCUS       DBMDVO010000673         1021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67530_length_1021_cov_0.883696, whole genome shotgun sequence.
ACCESSION   DBMDVO010000673 DBMDVO010000000
VERSION     DBMDVO010000673.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67530_length_1021_cov_0.883696"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="ACZPE2_08405"
     CDS             <1..855
                     /locus_tag="ACZPE2_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1319908.1"
                     /translation="NNIPMSYKIILDSCGELTEEMALSGHFTNVPLTLEVGEYRVVDD
                     ETFNQAAFLNAVASTTACPKSACPSPEQFMKAYDCDCSRIYVFTLSEQLSGSYNSAML
                     AKKLYEEINPYVKIYVFNSRSASAGETIEAAKVMEFEKQELSFEEIVEKTEEYISSIS
                     TYFVLEDLSFLERNGRLTGVKKIAANLLHIVPIMAGSKEGTIYQVGQARGINKALTKL
                     INLICEECEKKKTQTLIIAQCNCMGRALDIRERILAVVPNLKIVINATRGVSSLYAGN
                     GGIIFAFD"
BASE COUNT          331 a          190 c          207 g          293 t
ORIGIN      
        1 aataatatac ctatgtcata taagattatt cttgacagct gcggcgaatt aaccgaagaa
       61 atggctttaa gcggacattt taccaacgta ccgcttactc tcgaagttgg tgaatataga
      121 gttgttgacg acgaaacatt caatcaggct gcgtttctca atgcggttgc ttcaacaacg
      181 gcctgcccga aatcggcttg tccttctccc gaacagttta tgaaagctta cgactgcgat
      241 tgctctcgta tttatgtttt tactctttcg gaacagctca gcggctcata taacagtgca
      301 atgcttgcaa aaaaacttta tgaagaaatc aatccatatg ttaaaatata tgttttcaac
      361 agtcgttcag catctgccgg cgaaacaata gaagctgcaa aggttatgga attcgaaaaa
      421 caggagcttt catttgaaga gatagttgaa aaaaccgaag aatatatttc aagcatatca
      481 acatattttg ttcttgagga tttaagcttt cttgaaagaa acggccgtct taccggtgtt
      541 aaaaagattg ccgcaaacct tctgcatatc gttccgataa tggcaggaag caaagaaggc
      601 actatttatc aggtgggtca ggcaagaggc ataaataagg cactcacaaa gctgataaat
      661 ctcatttgcg aggaatgtga gaaaaagaaa acgcagactc ttatcattgc acagtgcaac
      721 tgcatgggca gagctttgga tattcgtgaa agaatacttg ccgttgttcc caatcttaaa
      781 atagtaatca atgcaaccag aggcgtttcg agcctttatg ccggaaatgg tggcattata
      841 ttcgcatttg attaataata caaattttaa ctgccttttt tataaaataa ggcagttttt
      901 ttatgtcata gacgtttaca gtcaaaaaag gagcatttat atggaaaaca acaaatactg
      961 gcttcacaga atttcacacg aatgggacgt agcttataag cttttatcgg aaggctggct
     1021 c
//
LOCUS       DBMDVO010000674         1019 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67681_length_1019_cov_1.161220, whole genome shotgun sequence.
ACCESSION   DBMDVO010000674 DBMDVO010000000
VERSION     DBMDVO010000674.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67681_length_1019_cov_1.161220"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="ACZPE2_08410"
     CDS             complement(<1..995)
                     /locus_tag="ACZPE2_08410"
                     /inference="COORDINATES: protein motif:HMM:NF037374.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HIR1319909.1"
                     /translation="MGVYDVLIKLSDSANYELLPYAAKLEIRNASQDSFMIDGIPAIS
                     YYGDEFALTADTDGTVTYTVTGADFDAATSSVRITKPGKVTVTAISSKPGFADKTAER
                     TFTAYKKAISVKVSAIDKIYDGATDVSFGAPVFTGTVSGDSVTADITGKMLNSDAGEN
                     KIVYADVSLSGNWGDYYTLDADSVQTTVNVAKAKITGIKITASDKAYDGTRDANAKVT
                     AIYGVLPIDEGAVKVTGTAAFDNVNAGIRTVLFVAEEITGAKGANYEFESGTAKIATY
                     IAQINKLKVKIFVSAATNRTYSGDPEYVDVSANAGGKVFTDFTVLYEDENGNAKEP"
BASE COUNT          215 a          292 c          239 g          273 t
ORIGIN      
        1 ggctctttcg cgttgccgtt ttcgtcctca tacaaaacgg tgaagtcggt aaataccttg
       61 ccgcctgcgt tcgccgaaac gtcaacatac tcaggatcgc ccgaatatgt gcggttcgtg
      121 gccgccgata cgaatatctt aaccttaagc ttgttgatct gtgctatata ggttgcaatt
      181 tttgcagtgc cggactcaaa ttcgtagttt gcgccctttg cgccggtaat ctcctccgca
      241 acgaataaga cagtcctgat gccggcattc acattgtcaa acgccgcggt tcccgtgact
      301 ttgaccgctc cctcatcaat cgggagaacg ccgtagatcg cggtgacctt cgcgttagcg
      361 tcccttgttc cgtcatacgc cttgtcggat gccgtaatct tgataccggt gatctttgcc
      421 tttgccacgt tgaccgtcgt ctgaacagag tctgcgtcca gcgtgtagta atctccccag
      481 ttgccggaaa gcgaaacatc cgcataaact atcttgttct cgcccgcgtc ggaattaagc
      541 attttccccg taatgtccgc cgtcacgcta tcacctgaaa ccgttccggt gaataccggt
      601 gcgccgaagg aaacgtccgt cgctccgtca tagatcttgt ctatagccga tacctttacc
      661 gagatcgcct ttttataagc ggtgaatgtt ctctctgccg tcttatccgc aaagcccggc
      721 tttgaactta tcgccgttac agtcaccttt ccgggtttgg tgatcctaac gcttgatgtt
      781 gcggcatcaa agtccgcgcc ggtaacggtg taggtaaccg taccgtccgt gtccgctgtt
      841 aaagcgaact cgtcgccgta gtagcttatc gccgggatgc cgtcaatcat aaacgaatcc
      901 tgggacgcgt ttcttatctc aagcttagcc gcatagggca aaagctcata atttgccgag
      961 tcggaaagct tgatcaaaac atcataaact cccacatcaa cgggggtcac ctcgttgcc
//
LOCUS       DBMDVO010000675         1017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67857_length_1017_cov_1.250000, whole genome shotgun sequence.
ACCESSION   DBMDVO010000675 DBMDVO010000000
VERSION     DBMDVO010000675.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67857_length_1017_cov_1.250000"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1017
                     /locus_tag="ACZPE2_08415"
     CDS             <1..>1017
                     /locus_tag="ACZPE2_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1319910.1"
                     /translation="EYADKLLEGLKHVDYLPNIKAQQEHWIGKSRGAFVNFSLKDIPE
                     KLRIYTTRPDTLFGVTFMVIAPEHPIIDKYKDKIRNMDAIVDYRTECSKKTEFERTQL
                     VKDKTGVKIDGLTAINPVNGREIPVYISDYVMMGYGTGAIMAVPAHDDRDYDFAKKFG
                     IDIIEVIKGGNIDEAAYTGDGEMVNSGFLNGMDNKKDSIKKMIDYLTEKGIGEGGVQY
                     KMKDWAFNRQRYWGEPIPIIHCPKCGMVPVPYDELPLKLPNVENFEPGQDGESPLAKI
                     DSFVNCKCPVCGGDAKRETDTMPQWAGSSWYFLRYVDPHNDKTFADYDKLKYWMPVDW
                     YNGG"
BASE COUNT          288 a          242 c          264 g          223 t
ORIGIN      
        1 ccgagtatgc cgataagctc ctagagggcc tcaagcacgt tgactatctc cccaacatca
       61 aggctcagca ggagcattgg ataggcaaga gccgcggcgc gttcgtcaat ttctctctta
      121 aagatattcc cgaaaagctt aggatataca caactaggcc tgacactctc ttcggcgtta
      181 catttatggt aattgctccg gagcacccga taatcgacaa gtataaggat aaaatccgca
      241 atatggatgc gatagtcgat taccgcacag aatgcagtaa aaagacagag tttgagcgca
      301 ctcagctcgt caaggataag acgggcgtca aaattgacgg tcttaccgcg ataaaccccg
      361 taaacggcag ggaaataccg gtttatattt ccgactacgt aatgatgggc tacggcacgg
      421 gcgcaataat ggctgtgccg gctcacgatg accgcgacta tgatttcgcc aagaaattcg
      481 gtatagacat tatcgaagtc ataaagggcg gaaatatcga tgaggcggca tataccggcg
      541 acggcgaaat ggtaaattcc ggcttcctta acggtatgga caataaaaag gattccatca
      601 aaaagatgat agattatctc accgaaaaag gcatcggtga gggcggagtt cagtataaga
      661 tgaaggattg ggcgtttaac cgtcagcgct actggggcga gcctattccg atcatccact
      721 gtccgaagtg cggaatggtt cccgttccgt atgacgagct tccgctgaag cttccgaatg
      781 ttgaaaactt tgaacccggt caggacggcg agagccctct tgcaaagatt gactcgtttg
      841 taaactgcaa gtgccccgtc tgcggcggcg acgcaaagcg cgagacggac accatgcctc
      901 aatgggcagg ctcctcgtgg tacttcttaa gatatgttga cccgcacaac gataagacct
      961 ttgccgacta tgacaagctt aaatactgga tgccggtaga ctggtacaac ggcggta
//
LOCUS       DBMDVO010000676         1017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67876_length_1017_cov_1.034934, whole genome shotgun sequence.
ACCESSION   DBMDVO010000676 DBMDVO010000000
VERSION     DBMDVO010000676.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67876_length_1017_cov_1.034934"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="ACZPE2_08420"
     CDS             complement(<1..159)
                     /locus_tag="ACZPE2_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1319911.1"
                     /translation="MKANIAFVSLGCDKNRVDSEVMLGILRERGYTIVSDETEADIIV
                     VNTCCFIKD"
     gene            complement(183..>1017)
                     /locus_tag="ACZPE2_08425"
     CDS             complement(183..>1017)
                     /locus_tag="ACZPE2_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1319912.1"
                     /translation="LLIPVVTDPKKAAGALNWAVREMLQRYNDFAENSVRDIKGYNRL
                     KAEKGETDFMPQIVIIIDELADLMMAAPGEVEDSICRLAQMARAAGIHLIIATQRPSV
                     DVITGLIKANIPSRLAFAVSSGIDSRTILDSVGAERLLGKGDMLFNPVGMSKPVRIQG
                     AFVTDQEVESIVEFLKQHKSVEYNTAVLDEITSSVKTGGSEEHDEYYDRAIELVVNKE
                     KASVSMLQRQFRIGYNRAARLMDELEATGIVGPEDGSKPRKVLISKAEYNAAKNSEQN
                     E"
BASE COUNT          245 a          256 c          184 g          332 t
ORIGIN      
        1 gtctttaata aaacagcacg tatttacaac aataatatcc gcctctgttt catctgaaac
       61 gattgtatat cctctttctc tgagtatgcc gagcattact tcgctgtcta ccctgttttt
      121 atcacaccca agtgatacaa atgcaatatt tgctttcaaa ataatttacc tccgtaaaac
      181 gattattcgt tttgttcact gttttttgcg gcattgtatt ccgcctttga tataagaacc
      241 tttctcggct tactgccgtc ctctggtccg actattccgg ttgcctcaag ctcgtccata
      301 agtcttgccg cacggttgta gcctatcctg aactgtcttt gcagcatgga aaccgatgcc
      361 ttttctttat ttacaacaag ctctatcgct ctgtcataat attcgtcatg ctcttcactg
      421 ccgcctgttt taaccgatga tgttatttca tcaagcacgg cggtgttata ttccacagat
      481 ttatgttgct ttaaaaactc aacaatactt tcaacttcct ggtcagttac aaatgcaccc
      541 tgtattctga caggcttgct catgccgact ggattaaaga gcatatcgcc ctttccgaga
      601 agtctttctg ctccgaccga gtcaagtatt gttcttgagt caattcccga agaaaccgca
      661 aaagcaagtc ttgacggaat gttggccttg ataagacctg taataacgtc aaccgacggt
      721 ctttgtgtcg ctataataag atgtattcct gccgcacgag ccatctgtgc aagtcggcat
      781 atgctgtcct ctacttctcc gggagccgcc atcataaggt cggcaagctc gtcgataata
      841 attactatct gcggcataaa gtctgtttcg cctttttccg ctttcagtct gttatagccc
      901 tttatatccc ttacggaatt ttcggcaaaa tcattatatc tctggagcat ttcccttaca
      961 gcccagttaa gtgcaccagc cgcttttttc ggatcggtaa caacaggtat gagcaga
//
LOCUS       DBMDVO010000677         1016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67975_length_1016_cov_0.845902, whole genome shotgun sequence.
ACCESSION   DBMDVO010000677 DBMDVO010000000
VERSION     DBMDVO010000677.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67975_length_1016_cov_0.845902"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="ACZPE2_08430"
     CDS             complement(<1..529)
                     /locus_tag="ACZPE2_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319913.1"
                     /translation="MNNEYATVKNCYNYGNVGNGGETHTGAIIGWCRNSNNQTSRFAD
                     NYYLRRSAPAGIGSGSKTLSGSAAPAEKNKSAFASGEVCYLVNGKTSKDSDAIWRQNI
                     DNNITPYDDYPLFDSAIVYYRSDGTYSNYKEDVQVDISWGAMSFKYTDGKWNPDEHSY
                     DEGSWEPAADDANKIT"
     assembly_gap    614..623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<624..906)
                     /locus_tag="ACZPE2_08435"
     CDS             complement(<624..906)
                     /locus_tag="ACZPE2_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319914.1"
                     /translation="MYGKITLPEENIYYDSRYNSVGGIAAKMDGGSISGVTSDIDIVN
                     TDGQYKHVGGIIGEIQNTTTNVTKCMYKGEIHVINSDDCVGGIVGYSNQG"
BASE COUNT          235 a          244 c          202 g          325 t
ORIGIN      
        1 ccgtgatttt attcgcgtca tctgcagccg gctcccacga tccctcatca tatgaatgct
       61 catcagggtt ccatttgccg tctgtgtatt taaaactcat agctccccag cttatatcta
      121 cctgaacatc ttctttatag ttgctgtagg taccgtcact gcggtagtaa acaatagctg
      181 aatcaaataa tggataatca tcatatggtg ttatgttgtt gtcaatgttc tgtctccata
      241 tcgcatcgga atctttgctg gttttgccgt ttaccagata gcatacttca ccgctggcaa
      301 aagctgattt gtttttttct gcaggtgcag ctgagccgct taaggtctta cttccgctgc
      361 cgattcccgc aggtgcagac cttctgagat aatagttgtc ggcaaaacgc gaagtctgat
      421 tattactgtt tctgcaccat cctatgattg cgcctgtatg ggtttcacca ccgttgccta
      481 cgtttccgta attgtaacaa ttttttacag tagcatactc attgttcaca taacctaata
      541 tccctccgct gtacggatct gaacctgcag taccctttac acaggcaaca gtgccaaggt
      601 ttgcgcaata actnnnnnnn nnncgccctg gttgctgtat cctacgatgc cgccgacaca
      661 gtcgtctgag tttataacgt gtatctctcc tttatacata cactttgtga cattagtagt
      721 agtgttttga atctctccga taataccgcc gacgtgtttg tactgaccgt ctgtgttgac
      781 aatgtcaatg tccgaggtta cgccggaaat cgaacccccg tccattttag cggcgatacc
      841 gccgacagag ttataacggc tgtcgtaata tatattttcc tcaggaagtg taatcttacc
      901 gtacacattg aagttttcca gtgttgcatt tgatacagtt ccaaagaaac cgggtcttaa
      961 atgggcacct gtaattttaa gccctgaaat tgactttttc gtttttgtgc ccgaga
//
LOCUS       DBMDVO010000678         1016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_67981_length_1016_cov_0.786885, whole genome shotgun sequence.
ACCESSION   DBMDVO010000678 DBMDVO010000000
VERSION     DBMDVO010000678.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67981_length_1016_cov_0.786885"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(51..854)
                     /locus_tag="ACZPE2_08440"
     CDS             complement(51..854)
                     /locus_tag="ACZPE2_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319915.1"
                     /translation="MIKLPKMYTANSIKAMHEKLNLDAQTVMLLYDYFDAFSNFYEML
                     PLKDAYKIIKKQNDKLKLTEEDFIAFSEIARHEEHFYFILGSEDLDKNRKKSKPMERT
                     IINESLVLIDEIFYKMMVASQKGKPLFVPEKNKLLRYVDDSFIEENEYTTALYDFFVN
                     NMKLGESDAWDTVGDCILEIKNGENPLEEVLSYLDYRKLLPEEKNLSELISIISRLNN
                     FTRTPVNRGFTPVELNQLGRGLSADSVVFEEDDLIPQAVFNSNSESSKQ"
BASE COUNT          285 a          218 c          160 g          353 t
ORIGIN      
        1 gccgttgacg cacatcacag cgccaacggc ttgctttata attatcctgt ttactgtttt
       61 gacgattcgc tgtttgaatt aaaaacggct tgcggtatca gatcatcttc ctcaaaaact
      121 acactgtcgg cagacagacc tctgccgagt tgattaagct caacaggtgt aaatccacga
      181 tttaccggtg ttctagtaaa gttattcaaa cgtgatataa tgcttatcaa ctcacttaga
      241 tttttctcct ccggcaaaag tttgcgataa tcaagatagc tgagcacttc ttcaagagga
      301 ttttcaccgt tttttatttc cagaatacaa tcgccgacag tatcccatgc atctgactcg
      361 cctaatttca tattattcac aaagaaatca tacagagccg ttgtatactc attttcttct
      421 ataaaagaat catcaacata tctgagcagc ttatttttct ccggaacaaa tagtggcttg
      481 cctttctgag aagcgaccat cattttataa aatatttcgt caataagcac caacgattcg
      541 tttatgattg ttctttccat cggcttactc ttttttctgt tcttgtccag atcttcactg
      601 ccgagtataa aatagaaatg ctcctcgtgc cttgctatct cagaaaaagc aataaaatct
      661 tcttccgtca gctttaattt atcattttgt tttttgatta tcttatatgc gtctttcaaa
      721 ggtaacatct cataaaaatt tgaaaacgca tcaaaataat catataacag cattactgtt
      781 tgtgcatcaa ggttaagctt ctcgtgcatc gctttaattg aatttgctgt atacatcttc
      841 ggtaatttta tcatagtaat ctcctttatc agagtttttc tgattatacc actttttgtc
      901 ggtaagtgca agttatgtgc gcaatcaaaa cgggctttga agcaacacat actccaaagc
      961 ccgtcagtta ttgttatatt caattgtcga atcggttatc ggcttactgc ctcgct
//
LOCUS       DBMDVO010000679         1014 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68120_length_1014_cov_1.272727, whole genome shotgun sequence.
ACCESSION   DBMDVO010000679 DBMDVO010000000
VERSION     DBMDVO010000679.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68120_length_1014_cov_1.272727"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="ACZPE2_08445"
     CDS             complement(<1..798)
                     /locus_tag="ACZPE2_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1319916.1"
                     /translation="MIIQHLRLDAINGSLVELDGVKDASFDEIVNFKLDDGTQRTGRI
                     VKIDGEHITVQVFEGTRGISLQNASTSLTGKPMEISLSPEILGRVFDGLGRPIDGLGE
                     VYPEEKRDVNGAPINPVSREYPRNFIQTGISSIDALATLIRGQKLPIFSGSGMKHNEL
                     AVQIVRQSTISDTEDFAIVFAAMGVKNDVANYFRTSFEEANVMNRVVMFLNLSNDPII
                     ERIMTPRCALTAAEYLAFTLHKHILVILTDMTSYAEALREFSSSKGEI"
     gene            complement(813..>1014)
                     /locus_tag="ACZPE2_08450"
     CDS             complement(813..>1014)
                     /locus_tag="ACZPE2_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1319917.1"
                     /translation="KMMHVILHLYHRAKEVVARQIPLSKITATGLFDKFTKMKYDVPN
                     SNIEMLDDYIKETDAVMDGILK"
BASE COUNT          258 a          237 c          224 g          295 t
ORIGIN      
        1 aatctcgccc tttgacgagc tgaattcacg aagagcctcg gcatatgacg tcatatcggt
       61 caaaattaca agtatgtgct tatgaagagt aaatgcgaga tattccgccg cggtaagagc
      121 gcagcgcgga gtcataattc tctcgattat agggtcgttt gaaaggttta agaacataac
      181 aaccctgttc attacatttg cctcttcaaa tgaagtgcgg aagtaatttg caacgtcgtt
      241 tttaacgccc attgccgcaa aaactattgc gaaatcctcg gtatcggaaa ttgtggactg
      301 acgcactatc tgaacggcga gctcgttatg cttcataccg gagcctgaga atataggcaa
      361 tttctgaccg cgtataagcg ttgcgagagc gtctatcgac gaaataccgg tctgaataaa
      421 gttacgcgga tattcacgtg atacggggtt tatcggagca ccgttaacat cgcgtttctc
      481 ttcgggatag acctcgccga gaccatctat gggtctgccg agtccgtcga acactctgcc
      541 gagaatttcg ggcgaaagcg atatttccat gggctttccg gtaaggcttg tggaggcgtt
      601 ctgaagagaa atacctcttg taccctcaaa cacctgtacc gtgatatgct caccgtctat
      661 cttaactatt cttccggtgc gctgtgttcc gtcgtcaagc ttaaaattaa ctatctcgtc
      721 aaacgaagcg tcctttacgc cgtcaagctc aacaagtgag ccgttgatag catcaagtct
      781 taaatgttga attatcattg tgctcaccta ccttacttca gtattccgtc cataacggca
      841 tcggtttctt tgatgtaatc gtcaagcatt tctatgttgc tgttcggcac atcgtatttc
      901 atctttgtaa acttatcaaa cagtcccgta gccgttatct ttgaaagagg aatctgtctt
      961 gcgactactt cctttgctct gtggtacaaa tgcagtatga cgtgcatcat ctta
//
LOCUS       DBMDVO010000680         1013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68233_length_1013_cov_0.796053, whole genome shotgun sequence.
ACCESSION   DBMDVO010000680 DBMDVO010000000
VERSION     DBMDVO010000680.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68233_length_1013_cov_0.796053"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="ACZPE2_08455"
     CDS             <1..358
                     /locus_tag="ACZPE2_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1319918.1"
                     /translation="AISNLVGLLLPRAIINPVVRLSGIKNSTKCNQLTKEERKRLAHT
                     IKNLKLTVLRFRPISEAVITSGGVDVNELSPKTMESKLCRNLYFAGEVIDVDAYTGGF
                     NLQIAFSTGFAAGNAQ"
     gene            379..>1013
                     /gene="cmk"
                     /locus_tag="ACZPE2_08460"
     CDS             379..>1013
                     /gene="cmk"
                     /locus_tag="ACZPE2_08460"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1319919.1"
                     /translation="MINVAIDGPAGAGKSTISRAAADKLGFIYVDTGALYRSVGVYAL
                     RNGINTKSGEQLKKELPDIKVELVFKDGVQHVYLNGEDVSEEIRTPKASMAASDVSAV
                     PEVRSFLFDLQRDIAVKNNCIMDGRDIGTVVLPNADVKIFLTASPESRAMRRYKELKE
                     KGASVGYDEVLADLKERDYNDSHRKIAPLKPAKDSITVDTTLFTLEESIKK"
BASE COUNT          311 a          192 c          244 g          266 t
ORIGIN      
        1 ggctatttca aatctcgtgg ggcttttgtt accgagagcc ataattaatc ccgttgtgcg
       61 cctttccgga attaaaaact caacaaagtg caatcaactc acaaaggaag agagaaaaag
      121 gcttgctcac acaattaaga atttaaagct gactgttctc agattcagac ctatcagcga
      181 ggctgttata acctctggcg gagtagatgt taatgagctt tctcccaaaa ccatggagag
      241 taagctgtgc cgtaatttgt attttgccgg tgaggttatt gatgttgatg catatacagg
      301 gggattcaac ctccaaatcg ccttttccac cggatttgcc gcaggcaacg cgcaataatg
      361 tatacaggag ataaaaatat gataaatgtc gctattgacg gtcctgcagg tgccgggaaa
      421 agcactattt caagagctgc tgccgacaag ctgggcttca tatatgtaga taccggagcg
      481 ttgtatcgct ctgtaggtgt ttatgcgttg aggaacggaa taaacacaaa atcaggcgaa
      541 cagcttaaaa aggaattacc cgatataaag gttgagctcg tctttaaaga cggcgtccag
      601 cacgtttatt taaacggcga ggatgtttca gaagaaatac gtacaccaaa agcttctatg
      661 gcggcttccg acgtttctgc cgtgccggag gtcagaagct ttctttttga tttgcaaaga
      721 gatatagcgg taaagaataa ttgtattatg gacggcaggg atataggaac agttgtattg
      781 cctaatgcag atgttaaaat attcttaacc gcatcgccgg agtcacgtgc catgagacgt
      841 tataaggagc ttaaggaaaa aggtgcttcg gttggttatg atgaggtttt agcagacctt
      901 aaagaacgag attacaatga ttcacataga aaaattgccc cgttgaagcc cgcaaaagac
      961 agcattacgg tggatacgac gctttttacg cttgaggagt caataaaaaa aat
//
LOCUS       DBMDVO010000681         1012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68277_length_1012_cov_1.212953, whole genome shotgun sequence.
ACCESSION   DBMDVO010000681 DBMDVO010000000
VERSION     DBMDVO010000681.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68277_length_1012_cov_1.212953"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1012
                     /locus_tag="ACZPE2_08465"
     CDS             <1..>1012
                     /locus_tag="ACZPE2_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1319920.1"
                     /translation="SFDILYKEISLKGNKALFVMADGMCNNMQVIWQVIGPVLFAPNM
                     PNEPSRQADYIRDRITTGADQSQTYDLDEAITDVLSGLIVLFIDGVDFCLEFGVQGFP
                     KRSVDDAKTEVQERGSHEGFTESFKDNAALLRRRVRSPIMKCETLTVGTTSKTRICVC
                     YMSDRASQETVDNIKARLKKAKLDMVLGSGYIAPFLDTQSTSFFSGVGTTERPDVACA
                     EMAEGRVVLLVDGTPFALIVPYLFVENFQSMDDYNYRPYYAVFIRTLKFISFLISVFF
                     PGIYVAVCTFHQEVLPSSMLYDMAVQESMTPFPVMVEAIFIHFLYEVMREAGLRMPKA
                     VGH"
BASE COUNT          245 a          200 c          280 g          287 t
ORIGIN      
        1 agctttgata tattgtataa ggaaatatcg cttaagggca acaaggcact ttttgttatg
       61 gcggacggta tgtgcaataa tatgcaggtg atatggcagg ttataggtcc tgttttgttt
      121 gcgccgaata tgccgaatga gccgtcaagg caggctgatt atatccgtga ccggataacc
      181 acgggagccg accagtcgca gacatacgat ttggacgaag cgataaccga tgtattgtcg
      241 ggacttatag tcctttttat cgacggagtt gatttttgcc ttgaattcgg tgtgcagggc
      301 tttccgaaaa gaagcgttga tgatgcaaag accgaggtgc aggagagagg ctcgcatgag
      361 gggtttacgg agagcttcaa ggataatgcg gcacttttac gccgcagagt ccgttcgccg
      421 ataatgaaat gcgaaacgct gactgtcggt acaacaagca aaacgagaat ttgcgtctgc
      481 tatatgtcgg acagagcgtc acaagagaca gtggacaata ttaaggcgcg tcttaaaaag
      541 gcaaagctcg atatggtact cggcagcggt tatattgcac cgtttctcga tacacaaagc
      601 acctcgtttt tttcgggcgt cggtaccacc gaacgcccgg atgttgcctg tgctgaaatg
      661 gcggagggga gagttgtact gcttgttgac ggtacgccgt ttgcgctgat tgtaccgtat
      721 ttgtttgtcg aaaacttcca atccatggat gactataact accgtccgta ttatgccgtg
      781 tttataagga cgctgaaatt tatatctttt ttgataagtg tgttttttcc cggtatttat
      841 gttgcggtat gtaccttcca tcaggaggtt ttgccgtcgt caatgctgta tgatatggcg
      901 gtgcaggaga gtatgacgcc gtttcccgtt atggtagagg ctatcttcat tcacttttta
      961 tatgaggtta tgcgtgaagc ggggcttcgt atgccaaagg ccgtcggcca cg
//
LOCUS       DBMDVO010000682         1012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68286_length_1012_cov_1.145993, whole genome shotgun sequence.
ACCESSION   DBMDVO010000682 DBMDVO010000000
VERSION     DBMDVO010000682.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68286_length_1012_cov_1.145993"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..160)
                     /locus_tag="ACZPE2_08470"
                     /pseudo
     CDS             complement(1..160)
                     /locus_tag="ACZPE2_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            282..632
                     /locus_tag="ACZPE2_08475"
     CDS             282..632
                     /locus_tag="ACZPE2_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319921.1"
                     /translation="MNSNGNTIKSVGFIAANTGAADAADAKAAVEKGTALPSAWKAAS
                     TDYVSQANASSDAYFGAIVKNISHASQATDIDCIAYVCYNDGTADHYVWYSAAVTAKV
                     ASGYDAAVAAWKAQ"
     gene            750..872
                     /locus_tag="ACZPE2_08480"
     CDS             750..872
                     /locus_tag="ACZPE2_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1319922.1"
                     /translation="MKQVYQTPDFDVSAYELDDIITISIGTGPVEGGDSGWVEI"
BASE COUNT          260 a          239 c          220 g          293 t
ORIGIN      
        1 ccattgcata catgataaac ggaatactcg gtgggatgat aacaccaagt ccgcccgcaa
       61 ctgcaacgat tgcggttgca aaatcttttt tatagccaag ctcgatcaga agcgggatcg
      121 tcatacttcc gactgctgcc acagttgcag gtgcagagcc gtcttatgct gtagctaagt
      181 ataagactca ggttaagttc acaggcgata agacaactgc tccggatgat ctcttccagt
      241 tcagacttac ttcggttatc acagctgctg atttcgctgc tatgaactca aacggcaata
      301 caattaagag cgttggcttc attgctgcca acacaggcgc tgccgatgca gctgatgcta
      361 aggcagcagt tgaaaaaggc accgcacttc cgtcagcttg gaaggctgct tctaccgact
      421 atgtaagtca ggcaaacgct tcttccgatg catacttcgg cgcaatcgtc aagaatatct
      481 ctcatgcttc tcaggcaact gatattgact gcatcgctta tgtatgctac aatgacggta
      541 ctgccgatca ttatgtttgg tattccgcag ctgtaactgc taaagttgct tcaggatatg
      601 atgctgctgt tgctgcttgg aaagctcagt aattgaattt taccaagaaa ccacagcgaa
      661 actttactat cggttcggct gttcaaaact gaatattccg ttccgacatt aaattttatt
      721 attatatctt gaaaggagat taaatctcaa tgaaacaggt ttatcaaaca ccggatttcg
      781 atgtttctgc atacgagctt gatgatatca tcacaatctc cataggcacc ggccccgtag
      841 agggcggcga ctcgggttgg gttgagatct gacgctcacg tagcaatcga tccgatttat
      901 taacaatcat aagcagagca aggttcgcct tgctctgttt ttttgtgccc tgttttgcat
      961 tttgtgcacc attccgtttc tgtaccccat tctgatatcg tgtgcctatt ct
//
LOCUS       DBMDVO010000683         1011 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68372_length_1011_cov_1.325275, whole genome shotgun sequence.
ACCESSION   DBMDVO010000683 DBMDVO010000000
VERSION     DBMDVO010000683.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68372_length_1011_cov_1.325275"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="ACZPE2_08485"
     CDS             <1..552
                     /locus_tag="ACZPE2_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HIR1319923.1"
                     /translation="VDTPDEAKQIIKELIKDDKLIVSGGTMTLKETGIQDFLNENYSG
                     IYIDREKLSPDEVNDLYRKAFSADTYFASTNAITEYGELYNVDGTGNRVAAMIFGPKQ
                     VIIVAGLNKIVTDLHSAVDRLETIASPANCVRLGRNTPCAKTGRCAHCLSPDRICNDY
                     VIMAHQRDKDRIKVIIISDEYGY"
BASE COUNT          317 a          222 c          220 g          252 t
ORIGIN      
        1 gtcgatactc ccgacgaggc taagcagata ataaaggagc ttataaagga cgacaagctg
       61 atagtttccg gcggaacaat gacgctcaaa gaaaccggca ttcaggattt tctgaacgaa
      121 aactacagcg gtatctacat tgaccgtgaa aagctctctc ccgatgaggt aaacgacctt
      181 tacagaaagg ctttttctgc cgacacctac tttgcaagca ccaatgcgat aaccgaatac
      241 ggcgaattat acaacgttga cggcaccggc aaccgtgtag ccgctatgat attcggaccg
      301 aagcaggtaa tcattgttgc gggcttaaac aagattgtaa cagacctcca ctcagccgtt
      361 gaccgtcttg aaacgatagc atctcccgca aactgcgtaa ggctcggcag aaacaccccc
      421 tgtgcaaaga caggcagatg tgcacactgc ttaagccccg acaggatttg caacgattat
      481 gtcataatgg ctcatcagcg tgacaaggac agaataaaag taatcatcat ctccgacgaa
      541 tacggctact aaggcggcaa tgtgccgcct caaattccga cctttttata ggttgtggat
      601 atatgcagga tagtgaaacg gtcgggaata aacgatagca aagatttatt tttactaata
      661 gcaatgtgcc gcctcaaatt ccgaacttgc ggcgagtcgc cgctgactgt tccgaccttt
      721 ttataggttg cagatatatg caggatagtg gaacggtcgg gaataaacga tagtaaagat
      781 ttatttttac taatagcaat gtgccgcttc aatacccgct ttttatagtt ccggatatat
      841 gcaggatagt gaaacggtcg ggattgaacg atagtgataa attaaaatat taaacacaaa
      901 acggtgggct tgtgaggctc accgttttat tcatctttat aaatattaat ataaccatac
      961 tatcagatta ttctgctcga tgcttgccaa tataatataa aaccacaaac c
//
LOCUS       DBMDVO010000684         1009 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68555_length_1009_cov_1.301762, whole genome shotgun sequence.
ACCESSION   DBMDVO010000684 DBMDVO010000000
VERSION     DBMDVO010000684.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68555_length_1009_cov_1.301762"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            216..455
                     /locus_tag="ACZPE2_08490"
     CDS             216..455
                     /locus_tag="ACZPE2_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1319924.1"
                     /translation="MRLDKFLKVSRIIKRRTVANEVCDAKHVNVNGKPARASYDVKEG
                     DVIEITFGQKVTKYQVLNVTEYATKESASSMYKEL"
     gene            457..909
                     /locus_tag="ACZPE2_08495"
     CDS             457..909
                     /locus_tag="ACZPE2_08495"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1319925.1"
                     /translation="MGYIEDLRKLIGHKRIVLNGSLVIIENKDGKILMQKRTYPKGKW
                     GLPGGLMELGESTVETAVREVREETNLTVKNLKLLGVYSGKDHLCKAENGDEWYVVVT
                     AYTTKDFSGTLMINDGESEALEWFCPEEIPKNIPSTHRLVIDDYKNGL"
BASE COUNT          330 a          195 c          233 g          251 t
ORIGIN      
        1 caagggtttt ttcgtccgcc tcacgacctt tatagccgag atagcgaaga acttcctttg
       61 tgtttatatc cataagtgaa acatcctctg tgccgaaaaa aatcgttatt acattctttt
      121 ttataataat acaaaagcag taggaattca acaatttaat ttcttgactt aaggcggctc
      181 ttattttaaa attaagctgt aaagggagtg aagaaatgcg acttgacaaa tttttaaagg
      241 tttcgcgtat cataaaaagg cgtactgtgg caaacgaggt gtgcgacgca aagcatgtca
      301 acgtaaacgg caagccggca agagcatctt acgatgtaaa agagggcgat gttattgaaa
      361 tcaccttcgg gcaaaaggtc acaaaatatc aggtcttgaa tgtaaccgaa tacgcaacaa
      421 aggaaagcgc gtcgtctatg tataaggagc tttaaaatgg gctatatcga agatttacgc
      481 aagctgatag gtcacaaaag aattgttctc aacggcagcc ttgtaatcat agaaaacaaa
      541 gacggaaaaa ttttgatgca aaaaagaaca tacccgaagg gcaagtgggg gcttcccgga
      601 ggtcttatgg aattgggcga gtcaaccgtt gaaacagctg taagagaggt tcgggaggaa
      661 actaatctta ccgtcaaaaa tctcaagttg ctcggtgtgt attccggtaa ggatcatctt
      721 tgcaaagcgg aaaacggcga cgaatggtat gttgtcgtga ccgcctacac cacaaaggat
      781 ttcagcggga cgcttatgat aaacgacggc gaatcggagg ctctcgagtg gttctgtccg
      841 gaagagatac cgaaaaacat cccgtctact caccgccttg tgatagacga ttacaaaaac
      901 ggcttataaa aacaggcttt ttaaaaataa aaaaggacta agctcttgct tagtcctttt
      961 ttggagctga taggcagact cgaactgccg acctcatcct taccaagga
//
LOCUS       DBMDVO010000685         1006 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68873_length_1006_cov_0.870718, whole genome shotgun sequence.
ACCESSION   DBMDVO010000685 DBMDVO010000000
VERSION     DBMDVO010000685.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68873_length_1006_cov_0.870718"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            375..>1006
                     /locus_tag="ACZPE2_08500"
     CDS             375..>1006
                     /locus_tag="ACZPE2_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1319926.1"
                     /translation="MENQSVKRGFWHKLFGTTPNDGNLQPREAIAYSITGIGQNFICT
                     IIGSYLTIFMTDALLMADARIGKTTGATAVALLMLFTRVYDALNDPIMGSIVDRTRTK
                     WGKCRPYLKWMAVPIGLVTIACFLPVYNSTAKSFIIISVIYVIWSMVFTVADVPYWGL
                     STAMTNNTTIRGNLLTVARLCCTIGAGAVTVFVPVITSAVTAKYKDADGVL"
BASE COUNT          289 a          210 c          206 g          301 t
ORIGIN      
        1 cattccgtag ctgaggtcaa gccccacggc acctacgccg tagcctatgt accccggtaa
       61 ttttttcagt aaactgtttg attttttcac gccgtcatcc tccgatttca gacaaataca
      121 aataatatat actctatata ataaatgttt aagagaaata agtcaaatag aaatatgcgg
      181 tttgaccgcc aaaaagggca tatcatttcc gaatgtgaaa aaataacggt cacaaacgag
      241 ttttgtgtct ttaatcaaat aaagatgaaa aatttgttgt attcaaattg actattttaa
      301 gtttctggat tataatattt tttatgactt tttaagttgt ataaagttgt aaaaaaggaa
      361 aaggagttaa cttaatggaa aatcaatctg ttaagagagg tttttggcat aagctgttcg
      421 gcaccacgcc gaatgacggt aatttgcagc cgcgcgaagc tattgcgtac agtattaccg
      481 gtataggcca aaactttatc tgcaccatca tcggctcata tcttaccata tttatgaccg
      541 atgcactttt aatggcggac gccaggatcg gcaaaacgac aggcgccacc gcagtagcgc
      601 ttcttatgct cttcacgagg gtgtatgatg ctctcaatga cccgattatg ggctctattg
      661 ttgacagaac aagaaccaaa tggggcaaat gccgcccgta tcttaaatgg atggctgttc
      721 cgataggctt ggttaccata gcctgcttcc tgcctgtcta taacagcacg gcaaaatcat
      781 tcatcattat ttcggttatc tatgttattt ggtcaatggt atttaccgta gccgatgttc
      841 cttattgggg cctttcaacg gctatgacaa ataacacaac cattcgcggt aatcttctta
      901 ctgttgcgcg tctttgctgc accataggcg caggcgccgt aacggtattt gtacccgtaa
      961 ttacttcggc tgtaaccgca aaatataagg atgccgacgg tgtgct
//
LOCUS       DBMDVO010000686         1006 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_68879_length_1006_cov_0.847514, whole genome shotgun sequence.
ACCESSION   DBMDVO010000686 DBMDVO010000000
VERSION     DBMDVO010000686.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68879_length_1006_cov_0.847514"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="ACZPE2_08505"
     CDS             <1..204
                     /locus_tag="ACZPE2_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1319927.1"
                     /translation="TMCDNTNMTKGVDEALKSRYGNLIFESRISKSVMASNSTAEQKA
                     MTINTKLGGQYAELTRELIERCK"
     gene            206..>1006
                     /locus_tag="ACZPE2_08510"
     CDS             206..>1006
                     /locus_tag="ACZPE2_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1319928.1"
                     /translation="MAFDFTNIKAKGKLNGLSSFSSLPAEDDTVKITPIPIDMIDPYT
                     ESLHPFKSYTVDKIDELAESIKVNGLLQPIIVSRKSDGRYMIIAGHNRFRACQKNNMD
                     TIDAVIRNELTATQRKLMMVDTNLEQRHKLTAKERALAYKIKQECLKELGATNPTAVI
                     AKENGESRKSVQRYIAISRLTPELLDMVDTDRIKLSVGVTVSAVDTETQNSLSEYLIS
                     EGEKATVDEKQAKEIQLEATVKPLSVDDIAEILEKKPQKEKKQSDIIKI"
BASE COUNT          368 a          197 c          225 g          216 t
ORIGIN      
        1 acaatgtgcg ataacacgaa tatgacaaaa ggtgttgacg aagcactcaa gagcagatac
       61 ggcaatctta tttttgagag ccgtatatca aaaagtgtta tggcgagcaa cagcacagcg
      121 gagcagaaag ctatgacgat aaacacaaaa ctcggcggac agtacgctga gctgacgaga
      181 gaacttatag aaaggtgcaa ataatatggc atttgatttt acaaatataa aggcaaaggg
      241 aaagctcaac gggctttcct ctttttcttc tctgccggct gaggatgata cagttaagat
      301 aacacctatt ccgatagata tgattgatcc gtacacggaa agcttgcacc cgtttaaatc
      361 gtacacagtc gataaaattg acgaacttgc tgagagcata aaggtcaacg gcttattaca
      421 gccgattatc gtgagccgta aatctgacgg acgctatatg attatagccg gacacaaccg
      481 tttccgtgcg tgtcaaaaga ataatatgga cactatagac gctgttatca gaaatgagct
      541 tacagccaca cagcgaaagc tgatgatggt tgacacaaac ctcgaacagc gacacaaact
      601 tactgccaaa gaacgtgcgt tagcatataa aatcaagcag gaatgtctta aagaactcgg
      661 agcaacgaat cccacagcag tgattgcaaa ggagaacggc gaaagccgta aatcagtaca
      721 gagatacatt gcaatcagca ggctaacacc ggaactgctc gatatggttg acacagacag
      781 aattaaatta tctgtcggcg ttacggtatc agctgtagat acagaaactc agaatagctt
      841 atcggagtat cttatcagcg agggcgaaaa ggcaaccgta gatgaaaaac aggctaaaga
      901 aatacagctt gaagcaacag ttaaaccgtt atcggttgat gatattgcag aaatccttga
      961 aaaaaagcca cagaaagaaa aaaagcaaag cgatataatc aaaatt
//
LOCUS       DBMDVO010000687         1004 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_69023_length_1004_cov_1.034330, whole genome shotgun sequence.
ACCESSION   DBMDVO010000687 DBMDVO010000000
VERSION     DBMDVO010000687.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69023_length_1004_cov_1.034330"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="ACZPE2_08515"
     CDS             complement(<1..352)
                     /locus_tag="ACZPE2_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR1319929.1"
                     /translation="MTEENLQEALKKFEALVREQSERSDRIKAQKDSIDYDKLDKIVI
                     GVCGGDGIGPIITKESARVLEYMLEDDVKAGKVEFKVIDGLTIENRIAHMQAIPDDVL
                     EELKSCHVILKGPTT"
     gene            complement(366..>1004)
                     /locus_tag="ACZPE2_08520"
     CDS             complement(366..>1004)
                     /locus_tag="ACZPE2_08520"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987189.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1319930.1"
                     /translation="NMIEMPASEEDADKVEVIYGPNIKPFPKTEKLPESIEAKALLKV
                     GDDITTDHIMPAGAKILPYRSNIPYLSQFCFGVCDKEFPDRCKKEGKGIIIGGANYGQ
                     GSSREHAALVPLYLGIKAVITKSFARIHCANLINAGILPLNFKNPDDYDKISEGDLLS
                     LEKVKDEILSDKPVMLLNETTGEKFELKYDLSTRQKDIILAGGLLNYTRESL"
BASE COUNT          246 a          213 c          202 g          343 t
ORIGIN      
        1 tcgttgttgg gccttttaaa ataacgtgac atgatttaag ctcttccaaa acatcatcgg
       61 gaatagcctg catatgggca attctgtttt cgattgtaag tccgtcaatg actttaaact
      121 caacttttcc ggctttaaca tcatcttcaa gcatatactc aagaactcgt gccgattctt
      181 ttgttataat cggtccaatg ccgtctccgc cgcaaacgcc gatgactatt ttatcaagtt
      241 tatcatagtc aatactgtcc ttttgagctt taattctgtc tgaacgttcc gattgctcgc
      301 gaacgagtgc ttcaaacttt tttaaagctt cctgcaaatt ttcttcagtc atttttatcc
      361 tcctattata atgattctct tgtgtagttg agaagaccgc ctgcaaggat aatatccttt
      421 tggcgtgttg acaaatcata tttaagctcg aacttttcgc cggttgtttc gttaagcaac
      481 atcacgggtt tgtctgagag tatctcgtct tttacttttt caaggctgag aaggtcgccc
      541 tcggaaatct tgtcgtaatc atcggggttt ttaaagttga gaggcaatat accggcatta
      601 ataagatttg cacagtgaat tcttgcaaat gattttgtga taactgcctt tataccgaga
      661 taaagcggaa ccaaagccgc atgttcacga gaagagccct gaccgtagtt agcgccgccg
      721 attataattc ctttgccttc ttttttacat ctgtcgggga attccttgtc gcacactccg
      781 aagcagaact gcgaaaggta aggtatattt gagcgatatg gtagtatctt ggcacctgcc
      841 ggcataatat gatcggtggt tatatcatct ccgactttga gaagtgcttt agcttcaatg
      901 ctctccggta atttttcggt ttttgggaac ggtttgatgt ttggaccgta aataacctca
      961 accttgtctg cgtcttcttc tgaagcgggc atttctatca tatt
//
LOCUS       DBMDVO010000688         1002 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Fimenecus sp. isolate MAG1228
            NODE_69204_length_1002_cov_1.177580, whole genome shotgun sequence.
ACCESSION   DBMDVO010000688 DBMDVO010000000
VERSION     DBMDVO010000688.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508327
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Fimenecus sp. (human gut metagenome)
  ORGANISM  Candidatus Fimenecus sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Candidatus Fimenecus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.178x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:26:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,705
            CDSs (total)                      :: 1,687
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 6 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Fimenecus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69204_length_1002_cov_1.177580"
                     /isolate="MAG1228"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:3022888"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            155..>1002
                     /locus_tag="ACZPE2_08525"
     CDS             155..>1002
                     /locus_tag="ACZPE2_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR1319931.1"
                     /translation="MSRYGVKFGLYKNNKFNERLFPFDTIPRIIPKNEFEFLEKGLKQ
                     RVYALNLFLNDIYSDKKIIRDKIIPEEFIYTSPGFSAPCDKLTPPKKIYNHISGIDLV
                     QGKDMCWYVLEDNLRIPSGASYPMIARELCRRASPDTFQNNSVDDNRDYGGLLRRVLD
                     DVNTGGINVILTPGRYNSAFFEHSYLAERTKAVFAMPQDLFVEGNVLYYSEYSGKRQR
                     VGTVYRRISDEYIDPMTFYAGSLLGVPHIIDSYRAGNVAIVNAPGNGIADDKGVYYFV
                     PQMIKY"
BASE COUNT          295 a          219 c          208 g          280 t
ORIGIN      
        1 gtttttgacg cttttacata ttgcaaatcc gtagatttat tatataatta taaaaacaga
       61 cttttatccg gttttgtttt tgtacaaaac atacatcaag tgaggtgaga acttgtacgg
      121 acttatagat catcaggaag aaattaaccg tcttatgtca cgttacggcg taaaattcgg
      181 actttataaa aacaacaaat tcaatgaacg gcttttcccc tttgacacca taccgcgcat
      241 aataccgaaa aacgaatttg aatttcttga aaaaggcttg aagcaacgcg tttacgctct
      301 taatttgttt ttaaacgata tttattccga caaaaaaatc atacgcgata aaataattcc
      361 cgaagaattc atatatactt cgccgggctt ttccgcaccg tgcgacaagc tgactccgcc
      421 taaaaagata tataatcaca tttcgggtat agatttggta caaggcaagg atatgtgctg
      481 gtatgtactt gaggacaatt tacgcatacc ctccggcgcg tcatatccga tgatagcgcg
      541 cgagctttgc cgcagagcaa gtcccgacac gttccaaaac aattcggtag acgacaaccg
      601 cgactacggc gggcttttgc gccgtgttct cgatgatgta aacaccggcg gaatcaacgt
      661 gattttgacc cccggccgat acaactcagc tttctttgag cattcgtacc ttgcggagcg
      721 cacaaaggct gtatttgcta tgccgcagga ccttttcgtt gaaggcaacg tgctttatta
      781 cagtgaatat tccggtaagc gacagcgcgt aggcacggtt taccgccgaa tttcagatga
      841 atatattgac cctatgacat tttacgcagg ctctcttctc ggagtaccgc atatcataga
      901 ttcttacaga gccggaaatg ttgcgattgt aaatgcgccc ggaaacggca tagcggatga
      961 caagggcgta tattatttcg taccgcaaat gataaaatat ta
//
